title: Expression and regulation of the bovine vesicular monoamine transporter gene.
abstract: In monoaminergic cells, the neurotransmitter is accumulated into secretory or synaptic vesicles by a tetrabenazine- and reserpine-sensitive transporter, catalyzing an H+/monoamine antiport. The major vesicular monoamine transporter from bovine chromaffin cells was cloned, using sequences common to adrenal medulla and brain rat vesicular monoamine transporters. Its identity was confirmed by peptide sequences, determined from the purified protein. Surprisingly, the bovine adrenal medulla sequence, bVMAT2, is more related to the transporter from human and rat brain than to that from rat adrenal medulla. PCR amplification showed that bVMAT2 is expressed in both adrenal medulla and brain, in contrast with the situation reported in rats, where distinct genes appear to be expressed in brain (SVAT or MAT, now renamed rVMAT2) and in the adrenal medulla (CGAT, now renamed rVMAT1). In bovine chromaffin cells, long-term depolarization by KCl resulted in an increase in the level of bVMAT2 mRNA, in agreement with the previously observed increase in the transporter binding sites, suggesting that a coupling between stimulation, secretion and synthesis changes the composition of the secretory granule membrane.
authors.affiliation: Laboratoire de Neurobiologie, CNRS Unité Associée 295, Ecole Normale Supérieure, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Krejci
authors.forename: B
authors.initials: B
authors.lastname: Gasnier
authors.forename: D
authors.initials: D
authors.lastname: Botton
authors.forename: M F
authors.initials: MF
authors.lastname: Isambert
authors.forename: C
authors.initials: C
authors.lastname: Sagné
authors.forename: J
authors.initials: J
authors.lastname: Gagnon
authors.forename: J
authors.initials: J
authors.lastname: Massoulié
authors.forename: J P
authors.initials: JP
authors.lastname: Henry
mesh_terms: D000313:Adrenal Medulla / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D015152:Blotting, Northern; D001923:Brain Chemistry; D002417:Cattle; D002838:Chromaffin System / Q000737:chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins
chemical_list: D018076:DNA, Complementary; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D012333:RNA, Messenger; C493451:Slc18a1 protein, rat; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins