title: IS204: an insertion sequence from Nocardia asteroides (mexicana) YP21.
abstract: An insertion sequence was found in Nocardia asteroides YP21. The element, designated IS204, is 1452 bp long and has 19/23 bp imperfect inverted repeats at its ends. The sequences of IS204 terminal inverted repeats have high homology with those of IS1096 from Mycobacterium smegmatis. An 8-bp duplication of target sequence was found at the insertion site. Sequence analysis revealed that IS204 contains an open reading frame of 1134 bp, which encodes a putative transposase similar to those found in IS1096 and in Tn4652 from Pseudomonas sp. EST1001. At least nine copies of IS204 are present in the genome of N asteroides YP21. The plasmid pCY 104::IS204 could be inserted with another copy of IS204 at a different site. The possible mechanisms are discussed.
authors.affiliation: Shanghai Institute of Plant Physiology, Academia Sinica, Peoples Republic of China.
authors.forename: W
authors.initials: W
authors.lastname: Yao
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Chiao
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics; D009616:Nocardia asteroides / Q000201:enzymology / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic; D019895:Transposases
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009713:Nucleotidyltransferases; D019895:Transposases