On the occurrence of somatic meiosis in embryogenic carrot cell cultures.
During the establishment of an embryogenic cell line from a carrot hypocotyl explant, processes closely resembling meiotic divisions are seen. A microdensitometric analysis revealed that the amount of cellular DNA diminished in the majority of cells to the haploid level. However, the diploid level was re-established in a matter of a few days. The genetic consequences of this segregation were studied by analyzing restriction fragment length polymorphisms (RFLP) and randomly amplified polymorphic DNAs (RAPD). The results showed that the great majority of embryos regenerated from segregants and that different segregants had different genetic constitutions.
Giorgetti
L
L
Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy.
Vergara
M R
MR
Evangelista
M
M
Lo Schiavo
F
F
Terzi
M
M
Nuti Ronchi
V
V
D001483:Base Sequence; D002460:Cell Line; D018744:DNA, Plant / Q000235:genetics; D018552:Daucus carota / Q000196:embryology* / Q000235:genetics*; D008540:Meiosis / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D018744:DNA, Plant