Increase of cell viability and P-glycoprotein expression in Chinese hamster ovary cells after 24 hours' exposure to vincristine.
Cell viability and P-glycoprotein expression in Chinese hamster ovary cells have been measured following incubation in a range of vincristine concentrations for 24 h. An increase of 12% in cell viability was observed with increasing drug concentrations in a drug-sensitive cell line (E29) and a lesser increase of 5% in a multidrug-resistant cell line (VRA15). The increased cell viability corresponded to a rise of P-glycoprotein level. Slot blots showed that there was an increase in P-glycoprotein mRNA with all drug concentrations tested in E29, although there was only partial correlation of mRNA levels with P-glycoprotein levels viability.
Vickers
S E
SE
Biology Department, University of York, UK.
Warr
J R
JR
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000818:Animals; D016466:CHO Cells / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects*; D002352:Carrier Proteins / Q000378:metabolism / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004351:Drug Resistance / Q000502:physiology; D004354:Drug Screening Assays, Antitumor; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors; D014750:Vincristine / Q000494:pharmacology*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D014750:Vincristine