title: The HOX-11 (TCL-3) homeobox proto-oncogene encodes a nuclear protein that undergoes cell cycle-dependent regulation.
abstract: Molecular analysis of the t(10;14) chromosomal translocation found in pediatric patients with T-cell acute lymphoblastic leukemia has led to the identification of the HOX-11 (TCL-3) protooncogene. The HOX-11 cDNA contains an open reading frame encoding a homeoprotein with features of DNA-binding. The majority of the t(10;14) chromosomal translocation breakpoints have been mapped to the 5' end of the HOX-11 gene, supporting the notion that deregulation of the HOX-11 gene by the t(10;14) chromosomal translocation contributed importantly to leukemia formation. To further define the role of the HOX-11 homeoprotein, we have prepared rabbit antiserum against a trpE-HOX-11 fusion protein. The purified anti-HOX-11 IgG immuno-precipitated a protein with apparent relative molecular mass of 40 kD. Biochemical fractionation demonstrated that the protein is localized in the nucleus. Furthermore, the HOX-11 RNA and protein appeared to be modulated during the cell cycle, with the highest level of expression at G1/S phase boundary. Taken together, these data suggest that the HOX-11 gene product may function as a transcription factor for G1 progression in the cell cycle.
authors.affiliation: Northeastern Ontario Regional Cancer Centre, Sudbury, Canada.
authors.forename: N
authors.initials: N
authors.lastname: Zhang
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Gong
authors.forename: M
authors.initials: M
authors.lastname: Minden
authors.forename: M
authors.initials: M
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology*; D002467:Cell Nucleus / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D016193:G1 Phase; D005786:Gene Expression Regulation / Q000235:genetics* / Q000502:physiology; D005801:Genes, Homeobox / Q000235:genetics*; D018398:Homeodomain Proteins; D006801:Humans; D007106:Immune Sera; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D015513:Oncogene Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D011233:Precipitin Tests; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011519:Proto-Oncogenes / Q000235:genetics*; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics; D016196:S Phase; D014157:Transcription Factors / Q000502:physiology; D014178:Translocation, Genetic / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D007106:Immune Sera; C000715470:MAS1 protein, human; D009687:Nuclear Proteins; D015513:Oncogene Proteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012334:RNA, Neoplasm; D014157:Transcription Factors; C071250:TLX1 protein, human