title: Diversity of neuronal phenotypes expressed in monolayer cultures from immature rabbit retina.
abstract: We have used monolayer cultures prepared from early postnatal rabbit retinae (days 2-5) by the sandwich technique to study the capacity of immature neurons to express specific neuronal phenotypes in a homogeneous in vitro environment. Applying morphological, immunocytochemical, and autoradiographic criteria, we demonstrate that a variety of phenotypes could be distinguished after 7-14 days in vitro, and correlated with known retinal cell types. Bipolar cell-like neurons (approximately 4% of total cell number) were identified by cell type-specific monoclonal antibodies (115A10) and their characteristic bipolar morphology. Small subpopulations (about 1%) of GABA-immunoreactive neurons acquired elaborate morphologies strikingly similar to those of A- and B-type horizontal cells. Amongst putative amacrine cells several different subpopulations could be classified. GABA-immunoreactive amacrine-like neurons (6.5%), which also showed high affinity [3H]-GABA uptake, comprised cells of varying size and shape and could be subdivided into subpopulations with respect to their response to different glutamate receptor agonists (NMDA, kainic acid, quisqualic acid). In addition, a small percentage of [3H]-GABA accumulating cells with large dendritic fields showed tyrosine-hydroxylase immunoreactivity. Presumptive glycinergic amacrine cells (18.5%) were rather uniform in shape and had small dendritic fields. Release of [3H]-glycine from these neurons was evoked by kainic and quisqualic acid but not by NMDA. Small [3H]-glutamate accumulating neurons with few short processes were the most frequent cell type (73%). This cell type also exhibited opsin immunoreactivity and probably represented incompletely differentiated photoreceptor cells. Summing the numbers of characterized cells indicated that we were able to attribute a defined retinal phenotype to most, if not all of the cultured neurons. Thus, we have demonstrated that immature neuronal cells growing in monolayer cultures, in the absence of a structured environment, are capable of maintaining or producing specific morphological and functional properties corresponding to those expressed in vivo. These results stress the importance of intrinsic factors for the regulation of neuronal differentiation. On the other hand, morphological differentiation was far from perfect indicating the requirement for regulatory factors.
authors.affiliation: Max-Planck-Institut für Hirnforschung, Frankfurt, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Möckel
authors.forename: S
authors.initials: S
authors.lastname: Löhrke
authors.forename: H D
authors.initials: HD
authors.lastname: Hofmann
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D002454:Cell Differentiation; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D018698:Glutamic Acid / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D007395:Interneurons / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000145:classification / Q000166:cytology* / Q000502:physiology; D010641:Phenotype; D011817:Rabbits; D012160:Retina / Q000166:cytology* / Q000502:physiology; D012165:Retinal Ganglion Cells; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005998:Glycine