C-terminal calcitonin gene-related peptide fragments and vasopressin but not somatostatin-28 induce miosis in monkeys.
The miotic effects of C-terminal calcitonin gene-related peptide (CGRP) fragments, somatostatin-28 and vasopressin have been evaluated with special attention being paid to possible interactions with cholecystokinin (CCK)A receptors. The peptides were injected intracamerally to anesthetized monkeys pretreated with indomethacin and atropine. CGRP-(32-37) induced a miosis with a potency 1000 times lower than that previously found with sulphated CCK-8. Two other fragments, CGRP-(30-37) and CGRP-(31-37), also had miotic properties. The CGRP-(32-37)-induced miosis was antagonized by the CCKA receptor antagonist loxiglumide. No contractile effect was elicited by 67 pmol-7.4 nmol somatostatin-28. Vasopressin (360 pmol) caused a small reduction in pupil size. Loxiglumide pretreatment did not affect the reduction in pupil size but a vasopressin receptor antagonist partly inhibited the response. The results indicate that CGRP-(32-37) is a miotic with low potency but high efficacy in the monkey eye, probably interacting with CCKA receptors, and that vasopressin is a mitotic with low potency and efficacy, probably acting via vasopressin receptors.
Almegård
B
B
Department of Physiology and Medical Biophysics, Uppsala University, Sweden.
Bill
A
A
D000704:Analysis of Variance; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D008252:Macaca fascicularis; D008297:Male; D015877:Miosis / Q000139:chemically induced*; D010446:Peptide Fragments / Q000494:pharmacology*; D011377:Proglumide / Q000031:analogs & derivatives / Q000494:pharmacology; D011680:Pupil / Q000187:drug effects*; D011949:Receptors, Cholecystokinin / Q000187:drug effects; D013004:Somatostatin / Q000494:pharmacology; D053561:Somatostatin-28; D013779:Tetrodotoxin / Q000494:pharmacology; D014667:Vasopressins / Q000494:pharmacology*
D010446:Peptide Fragments; D011949:Receptors, Cholecystokinin; C085123:calcitonin gene related peptide (32-37); D014667:Vasopressins; D013779:Tetrodotoxin; D013004:Somatostatin; D053561:Somatostatin-28; C053737:loxiglumide; D011377:Proglumide; D015740:Calcitonin Gene-Related Peptide