Visualization of taurine, GABA and glutamate in developing feline cerebellum by immunohistochemistry.
The localization of taurine, GABA and glutamate in developing feline cerebellum was performed using antibodies raised against the amino acids conjugated to bovine serum albumin with glutaraldehyde. Distinct patterns of immunostaining were observed for each of the amino acids. Taurine-like immunoreactivity reached a peak at 4 weeks after birth, as did GABA-like immunoreactivity, whereas glutamate-like immunoreactivity was greatest in the mature cerebellum. Purkinje cells are all taurine-positive in cerebellum from neonatal animals, whereas in the mature cerebellum they appear to contain only GABA and glutamate, with virtually no taurine, in contrast to observations reported with rodent cerebellum. Ultrastructural studies and immunogold labelling visualized by electron microscopy show that the band of taurine-like immunoreactivity observed in newborn feline cerebellum is localized within dendrites, axons and glial processes. Granule cells migrating through this region also show prominent taurine-like immunoreactivity.
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Department of Developmental Biochemistry, Institute for Basic Research in Developmental Disabilities, Staten Island, NY 10314.
Imaki
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H
Xu
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Sturman
J A
JA
D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000648:ultrastructure; D002415:Cats; D002531:Cerebellum / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D003712:Dendrites / Q000648:ultrastructure; D005260:Female; D005971:Glutamates / Q000276:immunology / Q000378:metabolism*; D018698:Glutamic Acid; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009457:Neuroglia / Q000648:ultrastructure; D011689:Purkinje Cells / Q000378:metabolism; D013654:Taurine / Q000276:immunology / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000276:immunology / Q000378:metabolism*
D005971:Glutamates; D013654:Taurine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid