Chemiluminescence and immunoassays.
The principles of chemiluminescence and the application of chemiluminescent labels and substrates in immunoassays are reviewed. Various immunoassay formats and all known classes of chemiluminescent molecules, including 1,2-dioxetanes, luminol and derivatives, acridinium esters, oxalate esters and firefly luciferins are described as well as the many sensitizers and fluorescent enhancers. Recent promising developments are discussed.
Rongen
H A
HA
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Utrecht University, The Netherlands.
Hoetelmans
R M
RM
Bult
A
A
van Bennekom
W P
WP
D000818:Animals; D006801:Humans; D007118:Immunoassay; D008163:Luminescent Measurements