title: Adhesion molecule expression and infiltrating maternal leucocyte phenotypes during blastocyst implantation in the pig.
abstract: During the implantation period (days 14 to 30 post coitum) in Large White pigs substantial numbers of endometrial sub-epithelial leucocytes were observed from about day 18 p.c. These were predominantly MHC Class II+, CD45+ and reactive for an accessory cell determinant carried by polymorphs and macrophages. Maternal lymphocytes of the CD2+, CD4-, CD8-, and a gamma delta TcR+ subtype identified by the mAb 86D, were also present. Few CD4+, CD8+, or double-positive lymphocytes were seen. CD2-CD4-CD8- gamma delta TcR+ "Null" cells were seen in stroma adjacent to endometrial glands, as were CD2+ lymphocytes. There was no apparent up-regulation of CD18, VLA-4 or CD25. E-selectin could not be detected on endothelium within uterine tissues or on trophectoderm, nor was a ligand for L-selectin detectable. The luminal aspect of the uterine epithelium was strongly reactive with anti-CD15 mAb, which was specifically blocked by lacto-N-fucopentaose III, indicating the presence of the LewisX determinant.
authors.affiliation: Department of Immunology, Babraham Institute, Cambridge, U.K.
authors.forename: A
authors.initials: A
authors.lastname: Whyte
authors.forename: R M
authors.initials: RM
authors.lastname: Binns
mesh_terms: D000818:Animals; D001755:Blastocyst; D018821:CD18 Antigens / Q000032:analysis; D018801:CD2 Antigens / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D004624:Embryo Transfer; D004717:Endometrium / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D004727:Endothelium / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D005260:Female; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000032:analysis; D007962:Leukocytes / Q000737:chemistry* / Q000166:cytology* / Q000502:physiology; D010641:Phenotype; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013552:Swine / Q000502:physiology*
chemical_list: D018821:CD18 Antigens; D018801:CD2 Antigens; D015704:CD4 Antigens; D016827:CD8 Antigens; D015815:Cell Adhesion Molecules; D015375:Receptors, Interleukin-2; D017493:Leukocyte Common Antigens