title: An extended panel of hamster-human hybrids for chromosome 2q.
abstract: A hamster-human hybrid containing only the q arm of chromosome 2 has been used to construct a panel of hybrids bearing reduced regions of chromosome 2 using the technique of irradiation fusion gene transfer. The human chromosome 2 carried the Ecogpt gene and all hybrids were selected using this marker. The integrated Ecogpt gene was localized to the region 2q33-34, resulting in the selective retention of this region in the hybrids. These data were combined with another previously constructed panel of hybrids containing regions of 2q, which were enriched for the region 2q36-37. The combined hybrid panel is useful for the mapping of new markers to defined regions of chromosome 2 and for the cloning of genes located on 2q by a positional strategy.
authors.affiliation: MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, UK.
authors.forename: M
authors.initials: M
authors.lastname: Hafezparast
authors.forename: C G
authors.initials: CG
authors.lastname: Cole
authors.forename: G P
authors.initials: GP
authors.lastname: Kaur
authors.forename: R S
authors.initials: RS
authors.lastname: Athwal
authors.forename: P A
authors.initials: PA
authors.lastname: Jeggo
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D006224:Cricetinae; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data
chemical_list: D005819:Genetic Markers