Studies on pharmacological activation of human immunoglobulin G by chemical modification and active subfragments. XIII. Effect of carboxamidemethylated Fc fragment (CM-Fc) on type II collagen-induced arthritis in DBA/1J mice.
The effect of carboxamidemethylated Fc fragment (CM-Fc) from human immunoglobulin G (IgG) on type II collagen (C II)-induced arthritis (CIA) in DBA/1J mice was studied. CM-Fc suppressed the development of CIA while Fc fragment did not. CM-Fc inhibited the increase in serum anti-C II IgG. CM-Fc suppressed C II-induced delayed type hypersensitivity (DTH) in DBA/1J mice. Moreover, the C II-induced proliferation of splenocytes from mice immunized with C II was suppressed by the administration of CM-Fc. Following an analysis of the lymphocyte population, a decreased percentage of B220+ cells and ratio of L3T4+/Ly-2+ cells was observed in lymph nodes of CM-Fc administered mice as compared with those of control group. These results suggest that CM-Fc inhibits CIA by regulating lymphocyte function.
Mimura
T
T
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Itoh
M
M
Gohgi
H
H
Suzuki
N
N
Takeuchi
K
K
Tsujikawa
K
K
Kohama
Y
Y
D000818:Animals; D001169:Arthritis, Experimental / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000276:immunology*; D003907:Dexamethasone / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D003907:Dexamethasone; D003094:Collagen