Meconium-induced lung injury mediated by activation of alveolar macrophages.
To clarify the mechanism of meconium-induced cellular injury, we examined the effect of meconium on the alveolar macrophages (AM) and bronchial epithelial cells (AK-D). Meconium obtained from healthy newborns was added to culture medium of AM and/or AK-D, which were cultured for 1 hour. Superoxide anion production of AM and the expression of intercellular adhesion molecule-1 (ICAM-1) on AK-D stimulated by meconium or oxygen radicals were determined. As a result, superoxide anion production of AM significantly increased when AM was cultured with meconium. Expression of ICAM-1 on AK-D appeared when AK-D was stimulated by hydroxyl radical but did not when AK-D was cultured with meconium. These results suggest that meconium-induced lung injury may occur through an activation of alveolar macrophages and the macrophage-epithelial cell axis may be important for the pathogenesis of meconium aspiration syndrome.
Kojima
T
T
Department of Pediatrics, Kansai Medical University, Osaka, Japan.
Hattori
K
K
Fujiwara
T
T
Sasai-Takedatsu
M
M
Kobayashi
Y
Y
D000818:Animals; D001980:Bronchi / Q000473:pathology / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis; D002478:Cells, Cultured; D004848:Epithelium / Q000473:pathology / Q000502:physiology; D006801:Humans; D007231:Infant, Newborn; D018799:Intercellular Adhesion Molecule-1; D008163:Luminescent Measurements; D008168:Lung / Q000473:pathology*; D008262:Macrophage Activation; D016676:Macrophages, Alveolar / Q000473:pathology / Q000502:physiology*; D008470:Meconium; D011817:Rabbits; D013481:Superoxides / Q000378:metabolism
D015815:Cell Adhesion Molecules; D013481:Superoxides; D018799:Intercellular Adhesion Molecule-1