Experimental evidence of a dual endocrine control of biosynthesis in the main nidamental glands of Sepia officinalis L. by factors from the central nervous system and the ovary.
1. A rapid, reliable and quantitative in vitro bioassay was developed to study the endocrine control of the biosynthesis of the egg capsule: incorporation of 14C-labelled D-glucose in polysaccharides and glycoproteins increased in dispersed-cell suspensions of main nidamental glands from maturing females. 2. Brain, optic lobes (OL) and ovary extracts from mature and maturing females stimulated the incorporation of 14C-labelled D-glucose in polysaccharidic and glycoproteic fractions of a nidamental cell suspension, whereas optic gland (OG) had no effect. 3. These results bring the first experimental evidence that one of the spawning events (egg-capsule edification) is controlled by the central nervous system and the ovary in a cephalopod.
Henry
J
J
Laboratoire de Biologie et Biotechnologies Marines, Université de Caen, France.
Boucaud-Camou
E
E
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D004702:Endocrine Glands / Q000502:physiology*; D005071:Evoked Potentials / Q000187:drug effects; D005260:Female; D005612:Freeze Drying; D006023:Glycoproteins / Q000378:metabolism; D008974:Mollusca / Q000502:physiology*; D010053:Ovary / Q000166:cytology / Q000502:physiology*; D011134:Polysaccharides / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology; D014818:Vitellogenesis
D006023:Glycoproteins; D011134:Polysaccharides; D014020:Tissue Extracts