Developmentally excised DNA sequences in Euplotes crassus capable of forming G quartets.
Tens of thousands of DNA segments are eliminated by DNA breakage and rejoining events during the formation of a new macronucleus in the hypotrichous ciliated protozoan Euplotes crassus. This study presents evidence for a class of eliminated sequences referred to as telomeric-repeat-like internal eliminated sequences (TelIESs). TelIESs are shorter (< 50 bp) than most previously characterized IESs and their DNA sequences resemble the telomeric repeat sequences of the organism. The TelIESs are excised during the developmental period of chromosome fragmentation/telomere addition, which is later than previously characterized IESs. Additional studies demonstrate that oligonucleotides representing the TelIESs are, like telomeric repeats, capable of forming G-quartet structures in vitro.
Klobutcher
L A
LA
Department of Biochemistry, University of Connecticut Health Center, Farmington 06030.
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D016812:Euplotes / Q000235:genetics*; D008745:Methylation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013463:Sulfuric Acid Esters; D016615:Telomere / Q000502:physiology
D017931:DNA Primers; D016054:DNA, Protozoan; D013463:Sulfuric Acid Esters; C007482:dimethyl sulfate