[A new variant of the simian T-lymphotropic retrovirus type I (STLV-IF) in the Sukhumi colony of hamadryas baboons].
Polymerase chain reaction (PCR) was developed for the detection of simian T-lymphotropic virus type 1 (STLV-1) infection of P. hamadryas and direct sequencing using oligo-nucleotide primer pairs specific for the tax and env regions of the related human T-lymphotropic virus type 1 (HTLV-1). Excellent specificity was shown in the detection of STLV-1 provirus in infected baboons by PCR using HTLV-1-derived primers. The nucleotide sequences of env 467bp and tax 159bp of the proviral genome (env position 5700-6137, tax position 7373-7498 HTLV-1, according to Seiki et al., 1983) derived from STLV-1-infected P. hamadryas were analysed using PCR and direct sequencing techniques. Two STLV-1 isolates from different sources (Sukhumi main-SuTLV-1 and forest stocks-STLV-1F) were compared. Two variants of STLV-1 among P. hamadryas with different level of homology to HTLV-1 were wound (83.8% and 95.2%, respectively). A possible role of nucleotide changes in env and tax sequenced fragments and oncogenicity of STLV-1 variants is discussed.
Chikobaeva
M G
MG
Schatzl
H
H
Rose
D
D
Bush
U
U
Iakovleva
L A
LA
Deinhardt
F
F
Helm
K
K
Lapin
B A
BA
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D006800:Deltaretrovirus Infections / Q000382:microbiology / Q000662:veterinary; D014644:Genetic Variation / Q000235:genetics*; D005846:Georgia (Republic); D008198:Lymph Nodes / Q000382:microbiology; D008214:Lymphocytes / Q000382:microbiology; D008969:Molecular Sequence Data; D010215:Papio / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D017422:Sequence Analysis, DNA; D015303:Simian T-lymphotropic virus 1 / Q000235:genetics*
D004279:DNA, Viral
Novyĭ variant obez'ian'ego T-limfotropnogo retrovirusa pervogo tipa (STLV-1F) v Sukhumskoĭ kolonii pavianov gamadrilov.