title: Polyamines alter intestinal glucose transport.
abstract: Polyamines are required for the growth of all eukaryotic cells. Enterocytes respond to luminal nutrients with large increases in polyamine synthesis, even though they are mature, nonproliferating cells. The role of polyamines in these cells is unknown. The current experiments examined whether polyamines affected intestinal transport of glucose, since absorption is the primary activity of enterocytes and since polyamines are known to affect membrane function and stability. Glucose transport was examined in rabbit brush-border membrane vesicles (BBMV). BBMV from rabbits given 5% alpha-difluoromethylornithine (DFMO) in their drinking water 24 h before they were killed transported significantly less glucose than control vesicles [38% decrease in maximal transport rate (Jmax)]. Orogastric administration of spermine, spermidine, or putrescine to DFMO-treated animals 24 h before they were killed prevented the decrease. In rabbits receiving only orogastric spermine, glucose transport was significantly increased (64% increase in Jmax), whereas in vivo spermidine and putrescine decreased Jmax. This increase in Jmax caused by in vivo administration of spermine was not dependent on protein synthesis. Addition of polyamines whether in vivo or in vitro decreased Michaelis constant in vesicles from control and DFMO-treated animals. The change in glucose transport induced by DFMO or polyamines was not related to altered membrane lipid composition or fluidity.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology and Biophysics, University of Tennessee College of Medicine, Memphis 38163.
authors.forename: L R
authors.initials: LR
authors.lastname: Johnson
authors.forename: P D
authors.initials: PD
authors.lastname: Brockway
authors.forename: K
authors.initials: K
authors.lastname: Madsen
authors.forename: J A
authors.initials: JA
authors.lastname: Hardin
authors.forename: D G
authors.initials: DG
authors.lastname: Gall
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D000518:Eflornithine / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects*; D007700:Kinetics; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis; D008871:Microvilli / Q000737:chemistry / Q000502:physiology; D011073:Polyamines / Q000494:pharmacology*; D011392:Proline / Q000378:metabolism; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology
chemical_list: D008563:Membrane Lipids; D011073:Polyamines; D003513:Cycloheximide; D011392:Proline; D012964:Sodium; D005947:Glucose; D000518:Eflornithine