title: Enantiomeric separation of drugs by affinity electrokinetic chromatography using dextran sulfate.
abstract: Dextran sulfate (sodium salt), which is a mixture of a linear alpha-(1,6)-linked D-glucose polymer having a sulfate group in the molecule, has been employed as a chiral selector in affinity electrokinetic chromatography (EKC) for the separation of enantiomers of drugs. Enantiomers of trimetoquinol hydrochloride and its positional isomer have been successfully separated by the method using a 3-5% dextran sulfate buffer solution of pH 5.5 within 20 min. The effects of buffer pH, concentration of dextran sulfate, and additives (such as an organic solvent, urea and a surfactant) on the enantioselectivity were investigated. The choice of pH and the concentration of dextran sulfate has been important for the improvement in selectivity.
authors.affiliation: Analytical Chemistry Research Laboratory, Tanabe Seiyaku Company, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nishi
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Nakai
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Terabe
mesh_terms: D002021:Buffers; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D016264:Dextran Sulfate / Q000737:chemistry; D004563:Electrochemistry; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D013237:Stereoisomerism; D014213:Tretoquinol / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D002021:Buffers; D016264:Dextran Sulfate; D014213:Tretoquinol