Ultrastructural changes in rat kidney mitochondria in response to the oxygen- or calcium-paradox.
Isolated, perfused rat kidney released lactate dehydrogenase in response to the protocol of a standard oxygen-paradox or calcium-paradox. The results are comparable to those found with rat heart. The mitochondria of the proximal tubule cells at the end of the calcium-paradox were swollen, had mitochondrial bars and were subdivided with internal septa; after the oxygen-paradox they exhibited, in addition, many myelin figures. Such ultrastructural changes were characteristic of the mitochondria of skeletal and cardiac muscles damaged by a rise in [Ca2+]i. It is concluded (i) that typical calcium- and oxygen-paradoxes can be produced in kidney, (ii) that these are associated with a rise in [Ca2+]i and (iii) the proximal tubules, in particular, are damaged.
Morton
J W
JW
Department of Environmental and Evolutionary Biology, University of Liverpool, U.K.
McLoughlin
H
H
Duncan
C J
CJ
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
D007770:L-Lactate Dehydrogenase; D010100:Oxygen; D002118:Calcium