Determination of a complete set of coupling constants in 13C-labeled oligonucleotides.
Three experiments are introduced to determine a complete set of coupling constants in RNA oligomers. In the HCCH-E.COSY experiment, the vicinal proton-proton coupling constants can be measured with high accuracy. In the P-FIDS-CT-HSQC experiment, vicinal proton-phosphorus and carbon-phosphorus couplings are measured that depend on the phosphodiester backbone torsion angles beta and epsilon. In the refocussed HMBC experiment, vicinal carbon-proton couplings are measured that depend on the glycosidic torsion angle chi.
Schwalbe
H
H
Institut für Organische Chemie, Universität Frankfurt, Germany.
Marino
J P
JP
King
G C
GC
Wechselberger
R
R
Bermel
W
W
Griesinger
C
C
D001483:Base Sequence; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*
D002247:Carbon Isotopes; D009843:Oligoribonucleotides