Solid-phase extraction and reversed-phase high-performance liquid chromatographic determination of sulphur mustard in blood.
A reversed-phase high-performance liquid chromatographic method is reported for the analysis of sulphur mustard in blood with the aid of solid-phase extraction sample preparation. Sulphur mustard is extracted from blood samples (both in vitro and in vivo) of rats with a solution of 0.05 M sodium dodecyl sulphate and pre-concentrated over Sep-Pak C18 cartridges pre-coated with Tween-20. A Polygosil C18 column is used with acetonitrile-water (52:48, v/v) as mobile phase for separation and sulphur mustard was detected at 200 nm.
Dangi
R S
RS
Defence Research and Development Establishment, Gwalior, India.
Jeevaratnam
K
K
Sugendran
K
K
Malhotra
R C
RC
Raghuveeran
C D
CD
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009151:Mustard Gas / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D009151:Mustard Gas