title: Interactions of ethanol and quinidine on contractility and myocyte action potential in the rat ventricle.
abstract: The combined effects of ethanol and quinidine on cardiac electromechanical coupling are unknown, but both drugs affect cardiac conduction and can cause myocardial depression. Isolated left ventricular papillary and ventricular myocytes were used to assess the combined effects of quinidine and ethanol on the electrophysiologic and mechanical properties of rat myocardium. The combination of quinidine (1-300 microM) and ethanol (120-240 mg/dL) depressed active papillary muscle tension within the clinically useful concentration range. In electrophysiologic studies of isolated ventricular myocytes, quinidine prolonged the action potential duration at 50% (APD50) and 90% (APD90) repolarization, the absolute refractory period, and the relative refractory period, but decreased the maximum rate of change of depolarization in phase 0 (Vmax). When cells were exposed to ethanol (240 mg/dL) and quinidine (1.5 microM) together, a significant decrease in the quinidine-induced prolongation of the absolute refractory and relative refractory periods was seen. Additional changes in action potential parameters from the quinidine values included slight reductions in Vmax and in APD50 and APD90, but these reductions were not consistently displayed, nor were they statistically significant.
authors.affiliation: College of Pharmacy, Department of Psychiatry, School of Medicine, University of Michigan, Ann Arbor 48109-01065.
authors.forename: S K
authors.initials: SK
authors.lastname: Guthrie
authors.forename: D W
authors.initials: DW
authors.lastname: Wilde
authors.forename: R A
authors.initials: RA
authors.lastname: Brown
authors.forename: A O
authors.initials: AO
authors.lastname: Savage
authors.forename: B
authors.initials: B
authors.lastname: Bleske
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004347:Drug Interactions; D004562:Electrocardiography / Q000187:drug effects; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D006329:Heart Conduction System / Q000187:drug effects; D006352:Heart Ventricles; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology; D010210:Papillary Muscles / Q000166:cytology / Q000187:drug effects; D011802:Quinidine / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012032:Refractory Period, Electrophysiological / Q000187:drug effects
chemical_list: D004338:Drug Combinations; D000431:Ethanol; D011802:Quinidine