title: Purification and characterization of vibrio cholerae O139 fimbriae.
abstract: A Vibrio cholerae O139 (strain Al-1841) isolated from a patient with a cholera-like disease in Bangladesh predominantly produced new curved, wavy fimbriae (Al-1841 fimbriae) and small numbers of previously reported V. cholerae non-O1 S7-like pili. The former was purified and characterized. The molecular mass of the Al-1841 fimbrial subunit was less than 2.5 kDa, and it was immunologically different from that of V. cholerae non-O1 S7 pili. This novel fimbrial antigen was detected in all 182 Gram-negative strains from five genera tested but was absent from the Gram-positive bacteria tested. The purified Al-1841 fimbriae did not agglutinate human or rabbit erythrocytes.
authors.affiliation: Department of Bacteriology, University of the Ryukyus, School of Medicine, Okinawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamashiro
authors.forename: N
authors.initials: N
authors.lastname: Nakasone
authors.forename: Y
authors.initials: Y
authors.lastname: Honma
authors.forename: M J
authors.initials: MJ
authors.lastname: Albert
authors.forename: M
authors.initials: M
authors.lastname: Iwanaga
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001459:Bangladesh / Q000453:epidemiology; D002771:Cholera / Q000453:epidemiology / Q000382:microbiology*; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D006384:Hemagglutination; D006801:Humans; D015151:Immunoblotting; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D014734:Vibrio cholerae / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial