title: Uptake and metabolism of albumin by rodent incisor enamel in vivo and postmortem: implications for control of mineralization by albumin.
abstract: The distribution of albumin throughout enamel development in the rat mandibular incisor was investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and Western blotting employing an anti-rat albumin antibody. Intact albumin was detectable at all stages of enamel development but was most evident during late secretion/transition. Its concentration was subsequently reduced during the maturation stage. Albumin degradation products appeared during the transition/early maturation stage indicating that albumin breakdown preceded its removal. As albumin inhibits apatite crystal growth, its degradation and removal may be a necessary prerequisite for normal enamel crystal growth, perhaps reflecting a general mechanism for removal of residual endogenous matrix or adventitious crystal growth inhibitors. Additional studies revealed that the maturation stage was particularly susceptible to albumin influx postmortem. Albumin could therefore form part of the natural crystal growth control process, which, if not removed, could hamper maturation and lead to white spot hypoplasias.
authors.affiliation: Division of Oral Biology, Leeds Dental Institute, University of Leeds, UK.
authors.forename: C
authors.initials: C
authors.lastname: Robinson
authors.forename: S J
authors.initials: SJ
authors.lastname: Brookes
authors.forename: J
authors.initials: J
authors.lastname: Kirkham
authors.forename: R C
authors.initials: RC
authors.lastname: Shore
authors.forename: W A
authors.initials: WA
authors.lastname: Bonass
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000566:Amelogenesis / Q000502:physiology*; D000818:Animals; D001031:Apatites / Q000737:chemistry; D015153:Blotting, Western; D003460:Crystallization; D003720:Densitometry; D003743:Dental Enamel / Q000378:metabolism* / Q000473:pathology; D003744:Dental Enamel Hypoplasia / Q000209:etiology; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008297:Male; D008334:Mandible; D011180:Postmortem Changes; D051381:Rats; D017208:Rats, Wistar; D014074:Tooth Calcification / Q000502:physiology*
chemical_list: D000418:Albumins; D001031:Apatites