Adrenergic lipolysis in immobilized and perfused adipocytes.
The method of cellular immobilization and perfusion was applied to adipocytes. The lipolytic effect of isoprenaline, whose action is produced as a result of receptor-drug interaction, was followed. An agarose solution kept at at 37 degrees C was mixed 1:1 with the cell suspension. Thereafter, adipocytes were immobilized in the agarose threads. The lipolytic effect of 0.1 ml of isoprenaline (1 x 10(-4) mol/l), that was rapidly introduced to the cell perfusion inlet in a non-recirculating system, was monitored by assessing glycerol production. The immobilized and perfused adipocytes exhibited significant lipolytic activity. After reaching the maximum effect, 0.1 ml of propranolol (1 x 10(-3) mol/l) that was applied to the bioreactor inlet, abolished the isoprenaline effect. The present data demonstrate the potential applicability of immobilized perfused adipocytes for various kinds of studies.
Lincová
D
D
Institute of Pharmacology, First Medical Faculty, Charles University, Prague, Czech Republic.
Miseková
D
D
Farghali
H
H
Martínek
J
J
Hynie
S
S
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001709:Biotechnology / Q000379:methods; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods; D007545:Isoproterenol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D010477:Perfusion / Q000379:methods; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011921:Rats, Inbred WKY; D012685:Sepharose
D012685:Sepharose; D011433:Propranolol; D007545:Isoproterenol