cDNA approaches to isolation of the mouse mutant weaver gene.
The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a substracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a substrated cDNA library.
Hodes
M E
ME
Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis 46202.
Dlouhy
S R
SR
Wei
J J
JJ
Wang
Y
Y
Sangameswaran
L
L
Lazar
V
V
Triarhou
L C
LC
Ghetti
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002531:Cerebellum / Q000002:abnormalities; D006224:Cricetinae; D018076:DNA, Complementary; D015723:Gene Library; D005808:Genes, Recessive; D006579:Heterozygote; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008818:Mice, Neurologic Mutants / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013378:Substantia Nigra / Q000002:abnormalities; D013737:Testis / Q000002:abnormalities
D018076:DNA, Complementary