Expression of intercellular adhesion molecule 1 (ICAM-1, CD54) in colonic epithelial cells.
The expression of intercellular adhesion molecule-1 (ICAM-1, CD54) was examined in 16 surgically removed colonic tumours and two colonic carcinoma cell lines. Immunohistochemistry showed a varying percentage of ICAM-1 positive colonic carcinoma cells in 9/16 tissue specimens, while normal colonic tissue (apart from a slight reactivity of endothelial cells) was not stained. The presence of the ICAM-1 molecule on the cell surface and the expression of ICAM-1 mRNA were investigated for two colonic carcinoma cell lines. It was possible to enhance the expression of ICAM-1 considerably by incubating the cells in the presence of inflammatory cytokines in HT-29 and CaCo-2 cells. The responsiveness to either interferon alpha (IFN-alpha), tumour necrosis factor alpha (TNF-alpha), or interleukin 1 beta (IL-1 beta) treatment was different in each cell line. Interestingly, ICAM-1 is shed by colonic carcinoma cells because soluble sICAM-1 was detected in the cell culture supernatants. In comparison with normal serum samples, the mean value of sICAM-1 in 63 samples of patients with colonic carcinoma and in 20 cases of active inflammatory bowel disease is raised about twofold. It remains to be clarified what part both forms of ICAM-1 play in the course of colonic cancer, ulcerative colitis, and Crohn's disease.
Dippold
W
W
I Medizinische Klinik und Poliklinik, Johannes-Gutenberg-Universität, Mainz, Germany.
Wittig
B
B
Schwaeble
W
W
Mayet
W
W
Meyer zum Büschenfelde
K H
KH
D001483:Base Sequence; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000097:blood; D003106:Colon / Q000737:chemistry*; D003110:Colonic Neoplasms / Q000097:blood / Q000737:chemistry*; D004848:Epithelium / Q000737:chemistry; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood*; D018799:Intercellular Adhesion Molecule-1; D007413:Intestinal Mucosa / Q000737:chemistry; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis* / Q000097:blood; D014407:Tumor Cells, Cultured
D015815:Cell Adhesion Molecules; D009363:Neoplasm Proteins; D018799:Intercellular Adhesion Molecule-1