Bilateral seizure-related changes of extracellular glutamate concentration in hippocampi during development of amygdaloid kindling.
To monitor the seizure-related changes of extracellular hippocampal glutamate (Glu) concentration during the development of amygdaloid kindling, we used brain dialysates and an enzymatic cycling technique for Glu determination with a highly sensitive assay and high time resolution (1 min). The extracellular Glu level was transiently (for 3 min) enhanced after stimulus and returned rapidly to baseline. In partially kindled rats (stage 3), the extracellular Glu level during the first minute post stimulus was 2.5-3.5-fold that of baseline, while fully kindled rats exhibited about a 5-fold increase in Glu level. Amygdaloid kindling is accompanied by a progressive, transient, stimulus-induced enhancement of extracellular Glu levels during the first minute post stimulus in both hippocampi.
Ueda
Y
Y
Department of Psychiatry, Miyazaki Medical College, Japan.
Tsuru
N
N
D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D004567:Electrodes, Implanted; D005110:Extracellular Space / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006624:Hippocampus / Q000378:metabolism*; D007696:Kindling, Neurologic / Q000502:physiology*; D008297:Male; D017551:Microdialysis; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D012640:Seizures / Q000378:metabolism*
D005971:Glutamates; D016194:Receptors, N-Methyl-D-Aspartate; D009243:NAD; D018698:Glutamic Acid