neu is not involved in N-[4-(5-nitro-2-furyl)-2-thiazolyl]-formamide-induced bladder carcinoma or 2-amino-4-(5-nitro-2-furyl)thiazole transformation of rat bladder epithelial cells.
Enhanced c-erbB-2/neu expression has been linked with a poor prognosis in human bladder cancer. Previous reports have shown that a point mutation at nucleotide T2012 in the coding region of the transmembrane domain of the rat gene is sufficient to confer transformation potential on this gene. We examined the comparative levels of p185neu as well as the sequence around the hotspot (T2012) of the neu gene of rat bladder cells transformed by 2-amino-4-(5-nitro-2-furyl)thiazole (ANFT) or established in culture from N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT)-induced rat bladder tumors. We concluded that increased p185neu expression did not correlate significantly with tumorigenicity. No alterations in nucleotide sequences of the neu gene were observed in either in vitro model.
Mann
A M
AM
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-3135.
Asamoto
M
M
Masui
T
T
Macatee
T
T
Eklund
S H
SH
Cohen
S M
SM
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002273:Carcinogens; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D066246:ErbB Receptors / Q000235:genetics*; D005200:FANFT / Q000031:analogs & derivatives; D015870:Gene Expression; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051381:Rats; D018719:Erb-b2 Receptor Tyrosine Kinases; D001743:Urinary Bladder / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000235:genetics*
D002273:Carcinogens; D066246:ErbB Receptors; D005200:FANFT; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; C024885:ANFT