title: Molecular cloning and sequence analysis of the monkey and human tissue kallikrein genes.
abstract: Cynomolgus monkey renal kallikrein cDNA and genomic human tissue kallikrein gene were cloned. The monkey gene encodes a 257 amino acid (aa) preprokallikrein and exhibits 95% and 92% homology to the human at nucleotide (nt) and aa level, respectively. The monkey gene encodes a 233-aa mature kallikrein versus a 238-aa in human. The human kallikrein gene and urinary kallikrein both contain a Lys-162 instead of the reported Glu-162. Human, monkey and rat renal/pancreatic kallikrein genes evolve with a N-glycosylation containing domain (aa 81-87) which is absent in porcine and is non-glycosylable in mice. Only human kallikrein evolves with an additional Thr-108 and with a N-glycosylation site at aa-141.
authors.affiliation: Amgen Inc., Amgen Center, Thousand Oaks, CA 91320.
authors.forename: F K
authors.initials: FK
authors.lastname: Lin
authors.forename: C H
authors.initials: CH
authors.lastname: Lin
authors.forename: C C
authors.initials: CC
authors.lastname: Chou
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: H S
authors.initials: HS
authors.lastname: Lu
authors.forename: W
authors.initials: W
authors.lastname: Bacheller
authors.forename: C
authors.initials: C
authors.lastname: Herrera
authors.forename: T
authors.initials: T
authors.lastname: Jones
authors.forename: J
authors.initials: J
authors.lastname: Chao
authors.forename: L
authors.initials: L
authors.lastname: Chao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D006801:Humans; D007610:Kallikreins / Q000737:chemistry / Q000235:genetics*; D007668:Kidney / Q000378:metabolism*; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007610:Kallikreins