Changes in the ADP-ribosylation status of some hippocampal proteins are linked to kindling progression.
The N-methyl-D-aspartate (NMDA) receptors are a class of excitatory amino acid receptors in the brain which are important for the induction of kindling and kindling-like phenomena. Post hoc sodium nitroprusside-induced ADP ribosylation of some proteins (particularly a p43 and a p39 protein) in homogenates from stimulated hippocampus was reduced at preconvulsive stage II and stage V (tonic-clonic seizures) of dentate gyrus kindling compared with controls. This effect, which probably reflects enhanced endogenous ADP ribosylation, depends on the progressive activation of the NMDA receptors and on the generation of nitric oxide (NO). The early occurrence and the persistence of these modifications suggest they may be associated to the long-lasting changes in neuronal function induced by kindling.
Vezzani
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Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
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Rizzi
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Zinetti
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Fratelli
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D015763:2-Amino-5-phosphonovalerate / Q000031:analogs & derivatives / Q000494:pharmacology; D000246:Adenosine Diphosphate Ribose / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism*; D007276:Injections, Intraventricular; D007696:Kindling, Neurologic / Q000502:physiology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D009599:Nitroprusside / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000378:metabolism
D006454:Hemoglobins; D009419:Nerve Tissue Proteins; D016194:Receptors, N-Methyl-D-Aspartate; D018048:Receptors, Purinergic P2; C064540:CGP 39551; D009599:Nitroprusside; D000246:Adenosine Diphosphate Ribose; D009569:Nitric Oxide; D015763:2-Amino-5-phosphonovalerate; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester