title: Neurotrophic effects of amnion explants co-cultured with rat mesencephalon.
abstract: We investigated amnion-derived neurotrophic effects on embryonic day 14 rat mesencephalic (E14M) explants in co-culture. E14M explants showed extensive neurite outgrowth directed toward amnion tissue. Tyrosine hydroxylase immunocytochemistry, western immunoblot and [3H]dopamine uptake studies revealed significant neurotrophic effects on E14M dopaminergic neurons over 14 days in culture. Thus, amnion tissue appears to have potent neurotrophic effects for embryonic mesencephalic dopaminergic neurons in vitro.
authors.affiliation: Department of Neurobiology and Anatomy, University of Rochester School of Medicine and Dentistry, NY 14642.
authors.forename: G M
authors.initials: GM
authors.lastname: Seigel
authors.forename: F
authors.initials: F
authors.lastname: Junn
authors.forename: D M
authors.initials: DM
authors.lastname: Gash
mesh_terms: D000650:Amnion / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D046508:Culture Techniques; D004298:Dopamine / Q000493:pharmacokinetics; D004622:Embryo, Mammalian / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000196:embryology* / Q000378:metabolism; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine