The adjuvant effect of interleukin-12 in a vaccine against Leishmania major.
Protection induced by vaccination depends on the capacity of the vaccine to elicit an appropriate immune response. In leishmaniasis, protection requires leishmanial-specific CD4+ T helper (TH) cells. Vaccination of BALB/c mice with leishmanial antigens and interleukin-12 (IL-12) promoted the development of leishmanial-specific CD4+ TH1 cells. These mice were resistant to subsequent infection with Leishmania major. Thus, IL-12 is an effective adjuvant for the initiation of protective cell-mediated immunity against leishmaniasis and may be an important component in other vaccines that need to induce cell-mediated immunity.
Afonso
L C
LC
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
Scharton
T M
TM
Vieira
L Q
LQ
Wysocka
M
M
Trinchieri
G
G
Scott
P
P
D000276:Adjuvants, Immunologic; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12; D015847:Interleukin-4 / Q000096:biosynthesis; D007378:Interleukins / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D018320:Leishmania major / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000517:prevention & control*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D016052:Protozoan Vaccines / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000953:Antigens, Protozoan; D007378:Interleukins; D016052:Protozoan Vaccines; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma