Effect of ovarian hormones on the hypothalamic excitatory amino acids system during sexual maturation in female rats.
The present experiments were designed to study in female rats during sexual maturation: (1) the hypothalamic release of aspartate (Asp), glutamate (Glu) and glycine (Gly) which are the excitatory amino acids (EAAs) involved in NMDA neurotransmission and of taurine (Tau), a putative inhibitory amino acid of GnRH secretion; (2) the relationships between the effect of estrogen-progesterone (EP) on the release of these EAAs and the secretion of gonadotropins, and (3) the effect of hypothalamic NMDA receptor stimulation on EAAs release by the hypothalamus as well as the effect of EP on this release. The release of EAAs by the anterior preoptic and medial-basal hypothalamic areas (APOA-MBH) is significantly higher in peripubertal than in prepubertal rats (p < 0.01). EP treatment in prepubertal rats (16 days of age) decreased LH and FSH plasmatic levels and also the in vitro release of Asp, Glu, Gly and Tau. Contrary to the observations in prepubertal rats, in 30-day-old peripubertal rats the ovarian hormones significantly (p < 0.01) increased the levels of LH and FSH as well as the release to the medium of these amino acids.(ABSTRACT TRUNCATED AT 250 WORDS)
Carbone
S
S
Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Szwarcfarb
B
B
Otero-Losada
M E
ME
Losada
M
M
Moguilevsky
J A
JA
D000818:Animals; D004338:Drug Combinations; D004967:Estrogens / Q000494:pharmacology*; D018846:Excitatory Amino Acids / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007033:Hypothalamus, Middle / Q000187:drug effects*; D007986:Luteinizing Hormone / Q000097:blood; D011301:Preoptic Area / Q000187:drug effects*; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D012741:Sexual Maturation / Q000502:physiology*; D013654:Taurine / Q000378:metabolism
D004338:Drug Combinations; D004967:Estrogens; D018846:Excitatory Amino Acids; D013654:Taurine; D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone