Immobilization of human thrombomodulin on biomaterials: evaluation of the activity of immobilized human thrombomodulin.
Thrombomodulin (TM) is a newly described endothelial cell associated protein that functions as a potent natural anticoagulant by converting thrombin from a procoagulant protease to an anticoagulant. In this study, the immobilization of hTM was investigated in detail using surface modified polymers. As the basis of immobilization, poly(acrylic acid) (PAAc) surface-grafted poly(ethylene) (PAAc-g-PE) film was used with the expectation of increasing the immobilization amount of hTM. The effect of the immobilization reaction on the hTM activities, and the comparison of the activities of the immobilized hTM with the free hTM, were studied.
Kishida
A
A
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
Ueno
Y
Y
Maruyama
I
I
Akashi
M
M
D000180:Acrylic Resins / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D001665:Binding Sites; D001672:Biocompatible Materials / Q000378:metabolism*; D001777:Blood Coagulation; D006801:Humans; D008969:Molecular Sequence Data; D011095:Polyethylenes / Q000378:metabolism*; D011486:Protein C / Q000378:metabolism; D018180:Thrombomodulin / Q000378:metabolism*
D000180:Acrylic Resins; D001672:Biocompatible Materials; D011095:Polyethylenes; D011486:Protein C; D018180:Thrombomodulin; C006903:carbopol 940