Effect of region, temperature and neuronal blockade on sodium and 51Cr-EDTA transport across canine gastrointestinal mucosae in vitro.
Muscle-stripped segments of canine stomach, ileum and colon have been used to identify regional differences in the movement of sodium and 51Cr-EDTA, and the effect that temperature has on the flux of these probes. Flux measurements revealed that the rate of movement of both probes followed the pattern ileum > colon > stomach. Analysis of flux ratios at 25 degrees C and 37 degrees C revealed that sodium moves actively and 51Cr-EDTA moves passively across gastrointestinal epithelia. Blockade of tonic neuronal activity did not affect the in vitro movement of either probe across the ileum.
Mckay
D M
DM
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
Ramage
J R
JR
Rangachari
P K
PK
Perdue
M H
MH
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002860:Chromium Radioisotopes; D004285:Dogs; D004492:Edetic Acid / Q000493:pharmacokinetics*; D005753:Gastric Mucosa / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D017136:Ion Transport / Q000187:drug effects; D009420:Nervous System / Q000187:drug effects; D012964:Sodium / Q000378:metabolism*; D013696:Temperature; D013779:Tetrodotoxin / Q000494:pharmacology; D014018:Tissue Distribution
D002860:Chromium Radioisotopes; D013779:Tetrodotoxin; D004492:Edetic Acid; D012964:Sodium