Is the lipolytic response in porcine adipose tissue slices equivalent to the lipolytic response in isolated adipocytes?
1. In most cases, when isolated adipocytes and adipose tissue slices from the same animal were stimulated with various lipolytic agents (adrenergic agonists, theophylline, adenosine deaminase), the qualitative response was similar. 2. There were, however, numerous exceptions; e.g. quinterenol did not affect isolated adipocytes whereas it was a partial agonist for adipose slices from the same animal. 3. The adipocytes present in slices were larger than those isolated from slices by collagenase digestion. 4. Isolated adipocytes were not more sensitive than tissue slices to stimulation by lipolytic agents.
Mersmann
H J
HJ
USDA/ARS Children's Nutrition Research Center, Houston, TX 77030.
Shparber
A
A
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D004983:Ethanolamines / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D066298:In Vitro Techniques; D008066:Lipolysis; D008297:Male; D013552:Swine
D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D005230:Fatty Acids, Nonesterified; C100139:quinterenol