title: Effect of somatolactin and related hormones on phosphate transport by flounder renal tubule primary cultures.
abstract: Winter flounder renal proximal tubule primary monolayer cultures mounted in Ussing chambers were used to determine the effect of salmon somatolactin (sSL) on transepithelial Pi and Ca2+ transport. sSL stimulated Pi reabsorption in a dose-dependent manner at physiological levels of the hormone (12.5 ng/ml). Net Pi transport was significantly altered by sSL (200 ng/ml) within 2 h after the initial exposure. Ca2+ fluxes were unchanged by the addition of 200 ng/ml sSL. The sSL-induced Pi reabsorption was abolished by 10 microM H-89, a highly specific protein kinase A inhibitor. Moreover the production and release of adenosine 3',5'-cyclic monophosphate were significantly increased after 1 and 2 h of exposure to sSL. The data indicate that sSL directly stimulates net renal Pi reabsorption by an adenosine 3',5'-cyclic monophosphate-dependent pathway. In addition to sSL, flounder SL and rat prolactin greatly, and salmon growth hormone (2.3 micrograms/ml) slightly, increased net Pi reabsorptive flux, whereas salmon prolactin had no effect.
authors.affiliation: Department of Physiology and Neurobiology, University of Connecticut, Storrs 06269.
authors.forename: M
authors.initials: M
authors.lastname: Lu
authors.forename: P
authors.initials: P
authors.lastname: Swanson
authors.forename: J L
authors.initials: JL
authors.lastname: Renfro
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D029941:Fish Proteins; D005432:Flounder; D006023:Glycoproteins / Q000235:genetics / Q000494:pharmacology*; D013006:Growth Hormone / Q000235:genetics / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects; D007546:Isoquinolines / Q000494:pharmacology; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism*; D010907:Pituitary Hormones / Q000235:genetics / Q000494:pharmacology*; D011388:Prolactin / Q000235:genetics / Q000494:pharmacology; D051381:Rats; D012474:Salmon; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013449:Sulfonamides
chemical_list: D029941:Fish Proteins; D006023:Glycoproteins; D007546:Isoquinolines; D010710:Phosphates; D010907:Pituitary Hormones; D013449:Sulfonamides; C064448:somatolactin protein, fish; D011388:Prolactin; D013006:Growth Hormone; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; D002118:Calcium