Single-channel mechanism of beta-adrenergic enhancement of cardiac L-type calcium current.
The cardiac L-type Ca2+ channel current is enhanced by beta-adrenergic stimulants by phosphorylation mediated by an increase in cyclic AMP. Single-channel studies have revealed that the Ca2+ channels are not always allowed to open at test steps from large negative holding potentials and beta-agonists increase the ratio of current-containing sweeps (availability, Ps). The duration of available (Ts) and unavailable states (TF) are measurable by applying a large number of test pulses. beta-Agonists shorten TF and prolong TS, possibly by enhancing channel phosphorylation by activating cyclic AMP-dependent protein kinase and by inhibiting channel dephosphorylation by phosphorylating phosphatase inhibitor 1, respectively, if the available state reflects the phosphorylated state of the channel. The modulation of the slow gating process, the increase in PS, plays a primary role in increasing the L-type Ca2+ current at moderate increase in sympathetic activity. When a larger increase in cyclic AMP is resulted, moduration of burst-kinetics including mode 2 openings also produced by phosphorylation enhances further the Ca2+ current.
Ochi
R
R
Department of Physiology, Juntendo University School of Medicine, Tokyo, Japan.
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D015640:Ion Channel Gating; D009206:Myocardium / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D000318:Adrenergic beta-Agonists; D015220:Calcium Channels; D011943:Receptors, Adrenergic, beta; D000242:Cyclic AMP