Structure of the human apolipoprotein D gene promoter region.
A human genomic clone of 18.2 kbp encompassing the apolipoprotein D (apoD) exon-1 and 2 and 10 kbp of upstream sequence was isolated and characterized. DNA sequencing and primer extension analysis revealed a transcriptional initiation site located 27 bp downstream of a consensus TATA box sequence. The exon 1 was 66 bp long. Computer analysis of DNA sequence from positions -557 to +129 revealed some putative transcriptional regulatory elements including a stretch of (pyrimidine/purine)26 located from nucleotide -263 to nucleotide -212, which could potentially form Z-DNA. Steroid hormone regulatory elements were identified which may be related to the modulation of apoD gene expression by androgens and estrogens in vitro.
Lambert
J
J
Département des Sciences Biologiques, Université du Québec, Montréal, Canada.
Provost
P R
PR
Marcel
Y L
YL
Rassart
E
E
D001053:Apolipoproteins / Q000235:genetics*; D053399:Apolipoproteins D; D001483:Base Sequence; D005091:Exons; D005809:Genes, Regulator; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D016385:TATA Box; D014158:Transcription, Genetic
D001053:Apolipoproteins; D053399:Apolipoproteins D; D009838:Oligodeoxyribonucleotides