title: opaque-15, a maize mutation with properties of a defective opaque-2 modifier.
abstract: An opaque mutation was identified that reduces gamma-zein synthesis in maize endosperm. The mutation, opaque-15, causes a 2- to 3-fold reduction in gamma-zein mRNA and protein synthesis and reduces the proportion of the 27-kDa gamma-zein A gene transcript. Although the protein bodies in opaque-15 are similar in size and morphology compared to wild type, there are fewer of them in developing endosperm cells. The opaque-15 mutation maps near the telomere of chromosome 7L, coincident with an opaque-2 modifier locus. Based on its phenotype, opaque-15 appears to be a mutation of an opaque-2 modifier gene.
authors.affiliation: Department of Plant Sciences, University of Arizona, Tucson 85721.
authors.forename: J M
authors.initials: JM
authors.lastname: Dannenhoffer
authors.forename: D E
authors.initials: DE
authors.lastname: Bostwick
authors.forename: E
authors.initials: E
authors.lastname: Or
authors.forename: B A
authors.initials: BA
authors.lastname: Larkins
mesh_terms: D018744:DNA, Plant / Q000032:analysis / Q000378:metabolism; D006579:Heterozygote; D007150:Immunohistochemistry; D008830:Microbodies / Q000502:physiology / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D009154:Mutation; D010940:Plant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D018749:RNA, Plant / Q000032:analysis / Q000378:metabolism; D012639:Seeds / Q000502:physiology; D013045:Species Specificity; D003313:Zea mays / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D015028:Zein / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant; D015028:Zein