title: Co-storage in large 'dense-core' vesicles of dopamine and cholecystokinin in rat striatum.
abstract: The subcellular localization of cholecystokinin in the striatum--an area where a high density of cholecystokinin containing terminals has been demonstrated--was studied using biochemical techniques. Cholecystokinin containing vesicles were partially purified using iso-osmotic Ficoll gradients. As judged from their size and their buoyant density in isopycnic gradients, cholecystokinin containing vesicles represent large 'dense-core' vesicles. Negative staining and subsequent immunolabelling for synaptophysin at the electron microscopical level, showed labelled vesicles of 50-70 nm. binding of dihydrotetrabenazine was detected in the cholecystokinin containing fractions. The results suggest that dopamine is co-stored with cholecystokinin in large dense vesicles in rat striatum.
authors.affiliation: Department of Medicine, University of Antwerp (UIA), Belgium.
authors.forename: I
authors.initials: I
authors.lastname: Llona
authors.forename: W G
authors.initials: WG
authors.lastname: Annaert
authors.forename: W
authors.initials: W
authors.lastname: Jacob
authors.forename: W P
authors.initials: WP
authors.lastname: De Potter
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002766:Cholecystokinin / Q000378:metabolism* / Q000502:physiology; D003342:Corpus Striatum / Q000378:metabolism* / Q000502:physiology; D004298:Dopamine / Q000378:metabolism* / Q000502:physiology; D005362:Ficoll; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D013395:Sucrose
chemical_list: D000906:Antibodies; D005362:Ficoll; D013395:Sucrose; D002766:Cholecystokinin; D004298:Dopamine