[A new autoantibody anti-Na which reacts with a cell cycle-dependent nuclear antigen].
By immunofluorescent techniques many autoantibodies have been detected in sera from patients with connective tissue diseases. A distinct antinuclear antibody reacting with a cellular component which showed a characteristic expression pattern is reported. The new antibody, termed anti-Na, stained approximately 20-30% of the HEp-2 cell with a variegated speckled pattern. In synchronized cell analysis, anti-Na showed fine speckles in G2/M phase cells, discrete speckles in dividing chromosomes, and specific cytoplasmic staining in anaphase and telophase cells. Although the preliminary study showed that 3% of SLE patients had anti-Na, the frequency of the anti-Na antibody in rheumatic diseases and their clinical significance remain to be clarified. Screening of a lambda gt11 cDNA expression library with anti-Na antibody yielded only one cDNA encoding a portion of this autoantigen. Deduced amino acid sequences of this cDNA showed a partial homology with myosin heavy chain of C. elegans, suggesting that the Na antigen is involved in contractibility related to myosin.
Okano
T
T
Hygienic Science Research and Development Center, Kitasato University, Tokyo.
Mimori
T
T
D000595:Amino Acid Sequence; D034961:Antigens, Nuclear; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D002453:Cell Cycle; D002478:Cells, Cultured; D003095:Collagen Diseases / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000276:immunology*; D018809:Proliferating Cell Nuclear Antigen; D017422:Sequence Analysis, DNA
D034961:Antigens, Nuclear; D001323:Autoantibodies; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen