Primary structure of mouse secretogranin III and its absence from deficient mice.
The rat brain- and pituitary-specific 1B1075 mRNA encodes a chromogranin/secretogranin-like protein, called secretogranin III (SgIII), that is a component of intracellular dense core vesicles. In order to study the function of this gene product in a mouse model system, we have isolated the murine homolog of the rat 1B1075 mRNA. This mRNA contains 2163 bp encoding a putative protein of 421 amino acids. Cleavage of the strong putative signal sequence would yield a mature protein of 51 kDa. The sequence of the encoded murine protein preserves the structural features that suggest SgIII is a member of the granin family, and allowed us to recognize and correct errors in our published rat sequence.
Dopazo
A
A
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Lovenberg
T W
TW
Danielson
P E
PE
Ottiger
H P
HP
Sutcliffe
J G
JG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001923:Brain Chemistry; D002864:Chromogranins; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D002864:Chromogranins; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger