Differential expression of the two types of histone H2A genes in wheat.
Five histone H2A cDNA clones have been isolated from a wheat cDNA library. They were divided into two groups, termed type 1 and type 2, based on their deduced amino acid sequences and their gene expression patterns. Three type 1 clones had ORFs encoding proteins similar to angiosperm histone H2As known so far, whereas two type 2 clones encoded an identical protein, which was more similar to Norway spruce (gymnosperm) H2A than to the angiosperm H2As. The C-terminus of the type 2 H2A was shorter than that of the type 1 H2As and lacked the characteristic SPKK motif that is conserved in angiosperm H2As. Northern analysis revealed that the mRNA levels of the type 1 H2A genes were high in proliferating cells during germination and in various tissues of young seedlings, while the mRNA levels of the type 2 genes were high in non-proliferating cells in which the type 1 gene was poorly expressed. This result suggests that the expression of these two groups of H2A genes is differently regulated during development in wheat.
Huh
G H
GH
Department of Botany, Faculty of Science, Kyoto University, Japan.
Matsuura
Y
Y
Meshi
T
T
Iwabuchi
M
M
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D018744:DNA, Plant; D006657:Histones; D010940:Plant Proteins