title: Casein-derived peptides can modulate the production of 5-hydroxyeicosatetraenoic acid in human neutrophils.
abstract: The effects of peptides derived from bovine casein hydrolysates were evaluated on oxygen-free radical release, 5-hydroxyeicosatetraenoic acid (5-HETE), and leukotriene B4 (LTB4) production by human polymorphonuclear leukocytes (PMNs). PMNs were activated by N-formyl-methionyl-leucyl-phenylalanine, phorbol myristate acetate, calcium ionophore (A23187), or opsonized zymosan. Tyrosyl-prolyl-phenylalanyl-proline (fragment 60-63 from bovine beta-casein) inhibited oxygen-free radical production and 5-HETE production but had no effect on the production of LTB4 and LTB4 isomers. Arginyl-tyrosyl-leucyl-glycyl-tyrosyl-leucine (fragment 90-95 from bovine alpha-casein) enhanced the production of oxygen-free radical and 5-HETE but had no effect on the production of LTB4 and isomers. Both peptides unsignificantly enhanced the release of arachidonic acid by A23187-stimulated PMNs previously supplemented with [14C]arachidonic acid. Casein-derived peptides then seemed to act on the enzymatic activities of endogenous peroxidases that reduced hydroperoxyeicosatetraenoic acid to 5-HETE. 5-HETE is recognized as an ubiquitous cell function mediator and modulation of PMN 5-HETE production by casein-derived peptides could be implicated in the manifestation of some adverse effects to cow's milk.
authors.affiliation: Institut national de la santé et de la recherche médicale, unité 58, Montpellier, France.
authors.forename: N
authors.initials: N
authors.lastname: Rabgaoui
authors.forename: M C
authors.initials: MC
authors.lastname: Guérin
authors.forename: J
authors.initials: J
authors.lastname: Torreilles
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002364:Caseins / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000378:metabolism; D004224:Diterpenes; D004723:Endorphins / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis*; D007975:Leukotriene B4 / Q000096:biosynthesis; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D013481:Superoxides / Q000097:blood; D013729:Terpenes / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D002364:Caseins; D003574:Cytochrome c Group; D004224:Diterpenes; D004723:Endorphins; D005609:Free Radicals; D006893:Hydroxyeicosatetraenoic Acids; D010446:Peptide Fragments; D013729:Terpenes; D013481:Superoxides; D007975:Leukotriene B4; D016718:Arachidonic Acid; C011309:mezerein; D000001:Calcimycin; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; C043158:beta-casomorphin 4; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate