Oxidative damage of mitochondria induced by Fe(II)citrate or t-butyl hydroperoxide in the presence of Ca2+: effect of coenzyme Q redox state.
The role of coenzyme Q on the process of mitochondrial membrane damage associated with oxidative stress was studied in a suspension of uncoupled mitochondria exposed to Ca2+ in the presence of Fe(II)citrate or t-butyl hydroperoxide. Reduction of coenzyme Q by succinate was shown to inhibit both inner membrane lipid peroxidation and permeabilization induced by Fe(II)citrate. In contrast, the inner membrane permeabilization induced by Ca2+ alone or Ca2+ plus t-butyl hydroperoxide was potentiated by the presence of succinate. These results support our previous proposition that the mitochondrial damage associated with oxidative stress generated by these pro-oxidants in the presence of Ca2+ is mediated by different mechanisms.
Castilho
R F
RF
Departamento de Bioquímica, Universidade Estadual de Campinas, SP, Brazil.
Kowaltowski
A J
AJ
Meinicke
A R
AR
Vercesi
A E
AE
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D019343:Citric Acid; D005296:Ferrous Compounds / Q000494:pharmacology*; D005609:Free Radicals; D008930:Mitochondria, Liver / Q000187:drug effects*; D008933:Mitochondrial Swelling / Q000187:drug effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010545:Peroxides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013386:Succinates / Q000378:metabolism / Q000494:pharmacology; D019802:Succinic Acid; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014451:Ubiquinone / Q000378:metabolism*; D020122:tert-Butylhydroperoxide
D005296:Ferrous Compounds; D005609:Free Radicals; D010545:Peroxides; D013386:Succinates; D017392:Thiobarbituric Acid Reactive Substances; D014451:Ubiquinone; D002084:Butylated Hydroxytoluene; D019343:Citric Acid; C016600:ferrous citrate; D000968:Antimycin A; D020122:tert-Butylhydroperoxide; D019802:Succinic Acid; D002118:Calcium