Chromosomal localization of the human vesicular amine transporter genes.
The physiologic and behavioral effects of pharmacologic agents that interfere with the transport of monoamine neurotransmitters into vesicles suggest that vesicular amine transport may contribute to human neuropsychiatric disease. To determine whether an alteration in the genes that encode vesicular amine transport contributes to the inherited component of these disorders, we have isolated a human cDNA for the brain transporter and localized the human vesicular amine transporter genes. The human brain synaptic vesicle amine transporter (SVAT) shows unexpected conservation with rat SVAT in the regions that diverge extensively between rat SVAT and the rat adrenal chromaffin granule amine transporter (CGAT). Using the cloned sequences with a panel of mouse-human hybrids and in situ hybridization for regional localization, the adrenal CGAT gene (or VAT1) maps to human chromosome 8p21.3 and the brain SVAT gene (or VAT2) maps to chromosome 10q25. Both of these sites occur very close to if not within previously described deletions that produce severe but viable phenotypes.
Peter
D
D
Department of Neurology, UCLA School of Medicine 90024.
Finn
J P
JP
Klisak
I
I
Liu
Y
Y
Kojis
T
T
Heinzmann
C
C
Roghani
A
A
Sparkes
R S
RS
Edwards
R H
RH
D000311:Adrenal Glands / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002879:Chromosomes, Human, Pair 10; D002898:Chromosomes, Human, Pair 8; D017124:Conserved Sequence; D006023:Glycoproteins / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides; D018377:Neurotransmitter Agents / Q000096:biosynthesis*; D051381:Rats / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013572:Synaptic Vesicles / Q000378:metabolism; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins
D006023:Glycoproteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; C493449:SLC18A1 protein, human; C493452:SLC18A2 protein, human; C493451:Slc18a1 protein, rat; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins