Detection of antibodies to Babesia bovis using an ELISA test.
An enzyme-linked immunoassay (ELISA) is described for the detection of antibodies to Babesia bovis using a partially purified antigen. The optimum conditions required to perform the test were determined and 210 serum samples were comparatively tested using ELISA and indirect immunofluorescence (IFA) tests, finding a diagnostic coincidence of 96.1%, a relative sensitivity of 96%, and a relative specificity of 96.4%.
Blandino
T
T
Parasitology Department, National Center of Animal and Plant Health, San José de las Lajas, Cuba.
Alonso
M
M
Barrera
M
M
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D016793:Babesia bovis / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay
D000913:Antibodies, Protozoan