Quantitative analysis of bencynonate in human plasma using a deuterated internal standard by gas chromatography-mass spectrometry with selected-ion monitoring.
A gas chromatographic-mass spectrometric method is described for the quantitative analysis of bencynonate in human plasma. Deuterated bencynonate served as the internal standard and selected-ion monitoring of the fragments of bencynonate and internal standard permitted the quantitation of bencynonate down to 25 pg/ml of plasma. The assay is linear for plasma bencynonate concentrations in the range 25 pg/ml-3 ng/ml. At 0.25 ng/ml the recovery and coefficient of variation are 54.3% and 19.1%, respectively. Application of the method to clinical studies gave data for the pharmacokinetics and relative bioavailability of bencynonate in man.
Liu
F
F
Institute of Pharmacology and Toxicology, Beijing, China.
Hu
X
X
Li
Q
Q
D000818:Animals; D001372:Aza Compounds / Q000097:blood* / Q000493:pharmacokinetics; D001682:Biological Availability; D001711:Biotransformation; D001643:Bridged Bicyclo Compounds / Q000097:blood* / Q000493:pharmacokinetics; D019086:Bridged Bicyclo Compounds, Heterocyclic; D018680:Cholinergic Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D051381:Rats
D001372:Aza Compounds; D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D018680:Cholinergic Antagonists; C092158:bencynonate; D003903:Deuterium