Localization of basic fibroblast growth factor in bovine adrenal chromaffin cells.
We have investigated basic fibroblast growth factor (FGF-2) localization in and release from isolated bovine adrenal chromaffin cells. In contrast to previous reports, we found no evidence of fibroblast growth factor (FGF) storage in catecholamine-containing chromaffin granules. Subcellular fractionation studies did not show enrichment of FGF-2 immunoreactivity in granules, and cholinergic stimulation failed to release FGF-2 into the medium. Our results suggest that adrenal chromaffin cells resemble other FGF-2-synthesizing cell types with respect to FGF storage and secretion.
Bieger
S C
SC
Department of Anatomy and Cell Biology, University of Heidelberg, Germany.
Henkel
A W
AW
Unsicker
K
K
D000313:Adrenal Medulla / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002417:Cattle; D002838:Chromaffin System / Q000166:cytology / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D013268:Stimulation, Chemical; D013347:Subcellular Fractions / Q000378:metabolism; D014018:Tissue Distribution
D016222:Fibroblast Growth Factor 2