Association of Hsp47, Grp78, and Grp94 with procollagen supports the successive or coupled action of molecular chaperones.
Hsp47, Grp78, and Grp94 have been implicated with procollagen maturation events. In particular, Hsp47 has been shown to nascent procollagen alpha 1(I) chains in the course of synthesis and/or translocation into the endoplasmic reticulum (ER). Although, Hsp47 binding to gelatin and collagen has previously been suggested to be independent of ATP. Grp78 and Grp94 are known to dissociate from its substrates by an ATP-dependent release mechanism. The early association of Hsp47 with procollagen and its relatively late release suggested that other chaperones, Grp78 and Grp94, interact successively or concurrently with Hsp47. Herein, we examined how these events occur in cells metabolically stressed by depletion of ATP. In cells depleted of ATP, the release of Hsp47, Grp78, and Grp94 from maturing procollagen is delayed. Thus, in cells experiencing metabolic stress, newly synthesized procollagen unable to properly fold became stably bound to a complex of molecular chaperones. In that Hsp47, Grp78, and Grp94 could be recovered with nascent procollagen and as oligomers in ATP depleted cells suggests that these chaperones function in a series of coupled or successive reactions.
Ferreira
L R
LR
Department of Pathology, School of Dentistry, University of Maryland at Baltimore 21201.
Norris
K
K
Smith
T
T
Hebert
C
C
Sauk
J J
JJ
D015082:2,2'-Dipyridyl / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002352:Carrier Proteins / Q000502:physiology; D002478:Cells, Cultured; D002847:Chromatography, Agarose; D003847:Deoxyglucose / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology; D000091342:Endoplasmic Reticulum Chaperone BiP; D050958:HSP47 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology; D006360:Heat-Shock Proteins / Q000502:physiology; D006900:Hydroxylation / Q000187:drug effects; D007535:Isomerases / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008565:Membrane Proteins / Q000502:physiology; D051379:Mice; D018832:Molecular Chaperones / Q000502:physiology*; D011233:Precipitin Tests; D011347:Procollagen / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D019704:Protein Disulfide-Isomerases
D002352:Carrier Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D050958:HSP47 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D008565:Membrane Proteins; D018832:Molecular Chaperones; D011347:Procollagen; C494661:Serpinh1 protein, mouse; C018551:glucose-regulated proteins; D015082:2,2'-Dipyridyl; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000255:Adenosine Triphosphate; D011392:Proline; D003847:Deoxyglucose; D007535:Isomerases; D019704:Protein Disulfide-Isomerases; D008239:Lysine