title: A rapid and simple method for the purification of transglutaminase from Streptoverticillium mobaraense.
abstract: Transglutaminase from Streptoverticillium mobaraense was partially purified by ion-exchange chromatography on a weak acid material and hydrophobic chromatography. The separation with a strong acid ion-exchanger produces homogeneous transglutaminase, in a single step and with high yields, directly from the centrifuged and filtered culture fluid of the micro-organism. The procedure reproduced several times could be also carried out on a larger scale with the optimized parameters of the laboratory isolations. The purified enzyme demonstrated good storage stability.
authors.affiliation: Fachbereich Chemische Technologie, Fachhochschule Darmstadt, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Gerber
authors.forename: U
authors.initials: U
authors.lastname: Jucknischke
authors.forename: S
authors.initials: S
authors.lastname: Putzien
authors.forename: H L
authors.initials: HL
authors.lastname: Fuchsbauer
mesh_terms: D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D013306:Streptomycetaceae / Q000201:enzymology*; D011503:Transglutaminases / Q000302:isolation & purification*
chemical_list: D011503:Transglutaminases