The presence of a calcium-sensitive p26-containing complex in bovine retina rod cells.
A protein with apparent M(r) 26 kDa (p26 [(1991) Biokimya 56, 225-228] or recoverin [(1991) Science 251, 915-918]) was suggested to activate GC in a calcium dependent manner in bovine retina rod cells [(1991) Science 251, 915-918; (1991) EMBO J, 10, 793-798]. However, according to our present data homogeneous p26 preparations do not activate the enzyme. At the same time we have revealed a complex of p26 with an unidentified protein, presumably RK, in bovine ROS. Calcium favours formation of the complex whereas EGTA addition (which corresponds to a low free Ca2+ concentration) leads to its dissociation.
Gorodovikova
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EN
Department of Enzymology, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
Philippov
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PP
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000502:physiology; D002417:Cattle; D005136:Eye Proteins / Q000032:analysis* / Q000502:physiology; D006162:Guanylate Cyclase / Q000378:metabolism; D051603:Recoverin; D017948:Retinal Rod Photoreceptor Cells / Q000737:chemistry*
D002135:Calcium-Binding Proteins; D005136:Eye Proteins; C067574:RCV1 protein, Bos taurus; D051603:Recoverin; D006162:Guanylate Cyclase; D002118:Calcium