title: Exercise-induced 31P-NMR metabolic response of human wrist flexor muscles during partial neuromuscular blockade.
abstract: The effects of a depolarizing (decamethonium, DECA) and a nondepolarizing neuromuscular blocking agent (vecuronium, VECU) on the phosphorus-31 nuclear magnetic resonance (31P-NMR)-detected metabolic response to muscle contractions were studied separately in six healthy untrained males. Subjects who showed splitting of the P(i) peak during graded rhythmic forearm exercise without the drugs were selected. It was found that both drugs abolished the P(i) peak splitting during exercise. Despite a similar reduction in phosphocreatine (PCr) during exercise with each drug, a smaller increase in P(i) was observed with DECA than with VECU (P < 0.05). End-exercise muscle pH was higher with DECA (6.93 +/- 0.07) than with VECU (6.79 +/- 0.11) (P < 0.05). The PCr and P(i) recovery was two- and threefold faster with DECA than with VECU, respectively (P < 0.05). On the basis of the concept that depolarizing and nondepolarizing agents have a preferential effect on fast- and slow-twitch muscle fibers, respectively, the present results support the hypothesis that the NMR-observed splitting of the P(i) peak reflects the metabolic differences between the two major fiber types of human skeletal muscle.
authors.affiliation: Copenhagen Muscle Research Center, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Mizuno
authors.forename: A
authors.initials: A
authors.lastname: Horn
authors.forename: N H
authors.initials: NH
authors.lastname: Secher
authors.forename: B
authors.initials: B
authors.lastname: Quistorff
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D003650:Decamethonium Compounds / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism*; D009466:Neuromuscular Blocking Agents / Q000494:pharmacology*; D010725:Phosphocreatine / Q000378:metabolism; D010758:Phosphorus; D005082:Physical Exertion; D012016:Reference Values; D014673:Vecuronium Bromide / Q000494:pharmacology; D014953:Wrist
chemical_list: D003650:Decamethonium Compounds; D009466:Neuromuscular Blocking Agents; D010725:Phosphocreatine; D010758:Phosphorus; D014673:Vecuronium Bromide; D000255:Adenosine Triphosphate; C033019:decamethonium