Effects of ageing on microglia in the normal rat brain: immunohistochemical observations.
The numbers of ramified microglia stained by mouse monoclonal antibodies (OX-6 and X-42) in various regions of the brain were compared in the young (8 weeks) and aged (12 months) rats. OX-6-positive microglia increased in number with ageing, but OX-42-positive microglia did not. In both young and aged rats, the number of OX-6-positive microglia in white matter was higher than that in grey matter, but the number of OX-42-positive microglia in white matter was the same as that in grey matter. OX-42 stains microglia in both activated and resting states, whereas OX-6 stains mainly microglia in the activated state. Our findings suggest that microglia become activated with age-related changes, especially in white matter.
Ogura
K
K
Department of Neurology, Jichi Medical School, Tochigi, Japan.
Ogawa
M
M
Yoshida
M
M
D000375:Aging / Q000473:pathology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001921:Brain / Q000166:cytology* / Q000254:growth & development*; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development; D007150:Immunohistochemistry; D008262:Macrophage Activation / Q000502:physiology; D008285:Major Histocompatibility Complex / Q000276:immunology; D008297:Male; D051379:Mice; D017628:Microglia / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal