Immunohistochemical investigations on the pyloric glands of the ruin lizard (Podarcis sicula campestris de Betta).
Mucous cells and enteroendocrine cells of the pyloric region of the ruin lizard (Podarcis sicula campestris De Betta) have been examined by lectin histochemical and immunohistochemical methods. Binding to five plant lectins (Canavalia ensiformis, Con A; Triticum vulgare, wheat germ, WGL; Lotus tetragonolobus, winged pea, WPL; Glycine max, soybean, SBL; Arachis hypogaea, peanut, PNL) was performed to characterize glycoconjugates in the secretory products of superficial and glandular mucous cells. Lectin histochemistry revealed the presence of N-acetyl-D-galactosamine, D-galactose and N-acetyl-D-glucosamine in the pyloric superficial cells. Mucous glandular cells mainly contained neutral glycoproteins with terminal residues of galactose, N-acetyl-D-glucosamine and N-acetyl-D-galactosamine. These cells did not react with Con A after periodate oxidation-sodium borohydride reduction (Paradoxical Con A staining). In the pyloric glands three different types of endocrine cells were identified immunohistochemically: gastrin-, serotonin- and somatostatin-immunoreactive cells; VIP-, bombesin- or cholecystokinin-immunoreactive cells have not been found in the pyloric mucosa.
Ferri
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Institute of Zoology and Comparative Anatomy, University of Bari, Italy.
Liquori
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D000818:Animals; D001839:Bombesin / Q000032:analysis; D002766:Cholecystokinin / Q000032:analysis; D005260:Female; D005755:Gastrins / Q000032:analysis; D006001:Glycoconjugates / Q000032:analysis*; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D008116:Lizards / Q000378:metabolism*; D008297:Male; D011708:Pylorus / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D012701:Serotonin / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D005755:Gastrins; D006001:Glycoconjugates; D037102:Lectins; D012701:Serotonin; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D002766:Cholecystokinin; D001839:Bombesin