Ketoprofen and prednisolone do not modulate neutrophil CR1, CR3 and Fc gamma RIII expression in healthy volunteers.
We assessed the effect of ketoprofen and prednisolone on the complement receptors (CR1 and CR3) and Fc gamma RIII expression on polymorphonuclears in an ex vivo study, using a randomized, single-blind, placebo-controlled, parallel design. Twenty-four healthy, male, Caucasian volunteers received either oral ketoprofen 100 mg twice daily, or prednisolone 5 mg twice daily, or placebo twice daily for 7.5 days. CR1, CR3 and Fc gamma RIII on unstimulated and FMLP-, C5a-, LTB4-, and GM-CSF-stimulated neutrophils were assessed using specific monoclonal antibodies and flow cytometry. No statistically significant drug effect was found for CR1, CR3, and Fc gamma RIII expression on polymorphonuclears. An in vitro study also yielded negative results. These findings do not support the hypothesis that the effect of non-steroidal antiinflammatory drugs on neutrophils is due to CR1, CR3, or Fc gamma RIII modulation.
Laroche
F
F
ECLIMED-Institut de Recherche Thérapeutique et Pharmacologique Cliniques, René Descartes University, Cochin Hospital, Paris, France.
Kahan
A
A
Kahan
A
A
Letrait
M
M
Cohen
C
C
Jamin
P
P
Strauch
G
G
D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D015936:Complement C5a / Q000494:pharmacology; D003198:Computer Simulation; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007660:Ketoprofen / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D007975:Leukotriene B4 / Q000494:pharmacology; D016177:Macrophage-1 Antigen / Q000096:biosynthesis / Q000187:drug effects*; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D016011:Normal Distribution; D011239:Prednisolone / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D017463:Receptors, Complement 3b / Q000096:biosynthesis / Q000187:drug effects*; D017452:Receptors, IgG / Q000096:biosynthesis / Q000187:drug effects*; D016037:Single-Blind Method; D044465:White People
D000911:Antibodies, Monoclonal; D016177:Macrophage-1 Antigen; D017463:Receptors, Complement 3b; D017452:Receptors, IgG; D007975:Leukotriene B4; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015936:Complement C5a; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007660:Ketoprofen; D011239:Prednisolone