[Riboflavin Reference Standard (Control 921) of National Institute of Health Sciences].
The raw material for riboflavin was tested for preparation of the "Riboflavin Reference Standard (Control 921)". Analytical data obtained were as follows: melting point, 283.8 degrees C (decomposition); specific absorbance, E1%1 cm = 852 (267 nm), 275 (373 nm), 325 (446 nm); infrared spectrum, the same as that of JP Riboflavin Reference Standard; optical rotation [alpha]20D = 138.5 degrees; thin-layer chromatography, no impurities were detected up to 6 micrograms; high-performance liquid chromatography, a small amount of 9 impurities were detected; loss on drying, 0.16%; assay, 100.3% by spectrophotometry. Based on the above results, the raw material was authorized as the Japanese Pharmacopoeia Reference Standard (Control 921).
Izumi
W
W
Kitajima
A
A
Yoshii
K
K
Komatsu
H
H
Ishimitsu
S
S
Okada
S
S
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006077:Government Agencies; D006296:Health Services; D007564:Japan; D010602:Pharmacopoeias as Topic; D012256:Riboflavin / Q000302:isolation & purification / Q000592:standards*; D013055:Spectrophotometry, Infrared
D012256:Riboflavin