Acrolein fixation for immunostaining tyrosine-hydroxylase in paraffin sections.
Routine histological fixatives barely preserve tyrosine-hydroxylase immunoreactivity in paraffin sections. fixation in 5% acrolein in phosphate buffer, pH 7.4, resulted in good preservation of the enzyme in the tissues investigated.
Iturriza
F C
FC
Center of Endocrine Studies, School of Medicine, National University of La Plata, Argentina.
Thibault
J
J
D000171:Acrolein; D000818:Animals; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D016612:Paraffin Embedding; D011817:Rabbits; D051381:Rats; D016707:Tissue Fixation / Q000379:methods*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*; D014649:Vas Deferens / Q000166:cytology / Q000201:enzymology
D000171:Acrolein; D014446:Tyrosine 3-Monooxygenase