The rat preprotachykinin-A promoter is regulated in PC12 cells by the synergistic action of multiple stimuli.
We demonstrate in PC12 cells that although nerve growth factor, forskolin or potassium-evoked depolarisation independently induced minimal or no expression from the rat preprotachykinin-A gene (rPPT) promoter linked to a reporter gene, exposure of the cells to various combinations of these stimuli specifically activated the rPPT promoter in transient transfection assays.
Morrison
C F
CF
MRC Brain Metabolism Unit, Royal Edinburgh Hospital, UK.
McAllister
J
J
Lyons
V
V
Chapman
K
K
Quinn
J P
JP
D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D004338:Drug Combinations; D004594:Electrophysiology; D005786:Gene Expression Regulation / Q000187:drug effects; D009414:Nerve Growth Factors / Q000494:pharmacology; D016716:PC12 Cells; D011188:Potassium / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000235:genetics*; D051381:Rats / Q000235:genetics*; D013268:Stimulation, Chemical; D015320:Tachykinins / Q000235:genetics*
D004338:Drug Combinations; D009414:Nerve Growth Factors; D011498:Protein Precursors; D015320:Tachykinins; C044206:preprotachykinin; D005576:Colforsin; D011188:Potassium