title: Evaluation of chemiluminescent detection of bromodeoxyuridine-labeled DNA.
abstract: Colorimetric immunoenzymatic procedures using monoclonal antibodies to detect 5-bromo-2-deoxy-uridine-DNA in hybridization experiments are not sensitive enough. We have developed a chemiluminescent immunodot assay to improve the sensitivity of the immunoenzymatic procedure, and attained a suitable detection level for single-stranded DNA. This new chemiluminescent immunodot assay has also been used to detect our recently synthesized cytidine analogue by means of its corresponding monoclonal antibody.
authors.affiliation: Department of Molecular Biology, Centro Nacional de Investigaciones Científicas, Havana, Cuba.
authors.forename: L
authors.initials: L
authors.lastname: López-Canovas
authors.forename: A
authors.initials: A
authors.lastname: López-Brauet
authors.forename: M D
authors.initials: MD
authors.lastname: Del Castillo-Bilbao
authors.forename: C
authors.initials: C
authors.lastname: Rodríguez-Tanty
authors.forename: D
authors.initials: D
authors.lastname: Higginson-Clarke
authors.forename: C
authors.initials: C
authors.lastname: Pascual-Márquez
authors.forename: A
authors.initials: A
authors.lastname: Macías-Cabrera
authors.forename: A M
authors.initials: AM
authors.lastname: Riverón-Rojas
mesh_terms: D001973:Bromodeoxyuridine; D003124:Colorimetry; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis*; D005069:Evaluation Studies as Topic; D015151:Immunoblotting; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization
chemical_list: D004277:DNA, Single-Stranded; D004247:DNA; D001973:Bromodeoxyuridine