Differential expression of homeobox-containing genes Msx-1 and Msx-2 and homeoprotein Msx-2 expression during chick craniofacial development.
The expression pattern of chick Msx-1 and Msx-2 homeobox genes in craniofacial primordia was examined by in situ hybridization using cRNA probes. Both genes were expressed in the distal region of the facial primordia, where the distribution of Msx-2 expression was restricted distally within the Msx-1 expression domain. On the contrary, Msx-2 expression in the lateral choroid plexus and cranial skull was broader and more intensive than Msx-1 expression. Our findings suggest that these two genes cooperate to play differential roles in craniofacial development. Msx-2 protein was detected immunohistochemically, and its localization essentially corresponded to the mRNA expression pattern, substantiating the involvement of Msx-2 protein as a transcriptional regulator in developing limb and face.
Nishikawa
K
K
Department of Biochemistry, Okayama University Dental School, Japan.
Nakanishi
T
T
Aoki
C
C
Hattori
T
T
Takahashi
K
K
Taniguchi
S
S
D000818:Animals; D002642:Chick Embryo; D002831:Choroid Plexus / Q000196:embryology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D005314:Embryonic and Fetal Development; D005147:Facial Bones / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D005786:Gene Expression Regulation; D005801:Genes, Homeobox; D018398:Homeodomain Proteins; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051957:MSX1 Transcription Factor; D009928:Organ Specificity; D012886:Skull / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor; C084119:MSX2 protein; D014157:Transcription Factors