Epidermal growth factor reverses antiandrogen induced cryptorchidism and epididymal development.
Epidermal growth factor, an androgen responsive paracrine factor, administered to pregnant mice has been reported to result in persistent wolffian ducts in female offspring. This fact led us to investigate whether epidermal growth factor can reverse the undescended testes and epididymal abnormalities associated with time specific flutamide administration. Timed pregnant Sprague-Dawley rats were treated with flutamide (undescended testes 74% and epididymal anomalies 53%) or flutamide plus epidermal growth factor (undescended testes 24% and epididymal anomalies 9%). The decrease in undescended testes and epididymal abnormalities following epidermal growth factor treatment was significant at p < 0.01. We performed immunohistological studies to evaluate whether flutamide alters epidermal growth factor expression in the paratesticular tissues during the time of maximal androgenic activity. These investigations revealed that antiandrogens did not alter epidermal growth factor expression in the fetal testes or epididymides. This finding suggests that epidermal growth factor does not reverse epididymal abnormalities or undescended testes by direct stimulation of the wolffian ducts or fetal testis.
Cain
M P
MP
Department of Urology, Mayo Clinic, Rochester, Minnesota.
Kramer
S A
SA
Tindall
D J
DJ
Husmann
D A
DA
D000014:Abnormalities, Drug-Induced / Q000517:prevention & control; D000818:Animals; D003456:Cryptorchidism / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control*; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D004822:Epididymis / Q000002:abnormalities / Q000196:embryology* / Q000378:metabolism; D005260:Female; D005485:Flutamide; D007150:Immunohistochemistry; D008297:Male; D011247:Pregnancy; D051381:Rats; D013737:Testis / Q000196:embryology / Q000378:metabolism
D004815:Epidermal Growth Factor; D005485:Flutamide