Microdialysis sampling coupled on-line to fast microbore liquid chromatography.
A system which combines intravenous microdialysis sampling on-line with fast microbore liquid chromatographic analysis is described. Using a 14 mm x 1 mm I.D. ODS column, caffeine, theobromine and paraxanthine could be resolved in under 1 min and acetaminophen and its two main metabolites, the glucuronide and sulfate conjugates, could be resolved in 30 s. This fast separation allowed on-line microdialysis sampling to be conducted with a 1-min sampling interval. The system was evaluated by monitoring the pharmacokinetics following intravenous administration of acetaminophen and caffeine.
Chen
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Department of Chemistry, University of Kansas, Lawrence 66045.
Lunte
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CE
D000082:Acetaminophen / Q000302:isolation & purification / Q000493:pharmacokinetics; D000818:Animals; D002110:Caffeine / Q000302:isolation & purification / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013805:Theobromine / Q000302:isolation & purification; D013806:Theophylline / Q000302:isolation & purification
D000082:Acetaminophen; D002110:Caffeine; D013806:Theophylline; D013805:Theobromine; C021183:1,7-dimethylxanthine