title: V delta 5+ T cells of BALB/c mice recognize the murine heat shock protein 60 target cell specificity.
abstract: The purpose of this paper was to study the gamma delta T-cell receptor repertoire and target specificity following stimulation of peripheral T cells of BALB/c mice with autologous or bacterial ligands. The expression of V gamma and V delta chain families in T cells that had been expanded by stimulation in syngeneic mixed lymphocyte culture or with purified protein derivative (PPD) was determined by the semiquantitative DNA polymerase chain reaction (PCR) method. Responder T cells to either of these stimuli strongly expressed both V delta 5 and V delta 6 genes. However, addition of the ML 30 anti-murine heat shock protein (hsp) 60 monoclonal antibody (mAb) to the cell culture selectively inhibited only the expansion of V delta 5 T cells. A V delta 5 T-cell hybridoma (KMT-5), which recognized syngeneic splenic and fibrosarcoma Meth A cells but not allogeneic cells, was produced by cell fusion from autoreactive blast cells. Incubation of the KMT-5 hybridoma in the presence of ML 30 antibody blocked the stimulation of interleukin-2 (IL-2) secretion by syngeneic target cells. It was also found that the DNA of KMT-5 hybridoma and of the autoreactive gamma delta T cells contained the BALB/c invariant delta (BID) chain sequence. It is concluded from these results that BALB/c peripheral V delta 5 T cells recognize an autologous hsp 60 target specificity in a V delta-gene restricted manner. We also propose that T cells of this V gene family may be involved in the immune surveillance of certain tumours and intracellular infections.
authors.affiliation: Department of Immunology, Kyushu University, Fukuoka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: G
authors.initials: G
authors.lastname: Matsuzaki
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshikai
authors.forename: R
authors.initials: R
authors.lastname: Seki
authors.forename: J
authors.initials: J
authors.lastname: Ivanyi
authors.forename: K
authors.initials: K
authors.lastname: Nomoto
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D018834:Chaperonin 60; D004247:DNA / Q000737:chemistry; D005260:Female; D015870:Gene Expression / Q000276:immunology; D006360:Heat-Shock Proteins / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology
chemical_list: D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D007135:Immunoglobulin Variable Region; D016692:Receptors, Antigen, T-Cell, gamma-delta; D014373:Tuberculin; D004247:DNA