Lipid determination from monophasic solvent mixtures: influence of uneven distribution of lipids after filtration and centrifugation.
Reasons for the variation in erythrocyte lipid extraction were investigated. We found that there was an uneven distribution of lipids after filtration (up to 10% accumulated in the filtrates) or centrifugation (up to 10% in the top fraction) of several apparently monophasic extraction systems. The uneven distribution was more pronounced in the systems containing relatively more water, or, generally speaking, with more nonpolar solvents. Ill-defined procedures using either filtration or centrifugation, therefore, could be the cause for variations in lipid extraction. A way to prevent the uneven distribution has been developed and is discussed.
Wang
W Q
WQ
Department of Cell Biology I, University Hospital of Lund, Sweden.
Gustafson
A
A
D000433:1-Propanol; D002498:Centrifugation; D002725:Chloroform; D004912:Erythrocytes / Q000737:chemistry*; D000431:Ethanol; D005374:Filtration; D006586:Hexanes; D006801:Humans; D008055:Lipids / Q000097:blood*; D000432:Methanol; D012997:Solvents
D006586:Hexanes; D008055:Lipids; D012997:Solvents; D000431:Ethanol; D002725:Chloroform; D000433:1-Propanol; D000432:Methanol