title: Characterization of Bacillus thuringiensis and related bacteria by ribosomal RNA gene restriction fragment length polymorphisms.
abstract: Ribosomal RNA gene restriction patterns have been determined for 43 strains of Bacillus thuringiensis representing 10 serovars and eight reference strains of B. anthracis, B. cereus and B. mycoides. Strains within a B. thuringiensis serovar produced highly related or identical ribotype patterns: in particular, 12 strains of serovar israelensis, five strains of serovar kurstaki, two strains of serovar galleriae and three strains of serovar aizawa produced ribotype patterns consistent with serotype designations. Moreover, variety tenebrionis (serotype 8a8b), a coleopteran pathogen, could be distinguished from the more common lepidopteran pathogens of this serotype (serovar morrisoni) by ribotyping. The correlation of ribotype patterns with serotype suggests a clonal population structure for B. thuringiensis.
authors.affiliation: Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh, UK.
authors.forename: F G
authors.initials: FG
authors.lastname: Priest
authors.forename: D A
authors.initials: DA
authors.lastname: Kaji
authors.forename: Y B
authors.initials: YB
authors.lastname: Rosato
authors.forename: V P
authors.initials: VP
authors.lastname: Canhos
mesh_terms: D001407:Bacillus / Q000145:classification / Q000235:genetics; D001413:Bacillus thuringiensis / Q000145:classification* / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D016000:Cluster Analysis; D004275:DNA, Ribosomal / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012703:Serotyping
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004275:DNA, Ribosomal; D004731:Endotoxins; D006460:Hemolysin Proteins; D012336:RNA, Ribosomal, 16S; C030956:insecticidal crystal protein, Bacillus Thuringiensis