Expression of the adhesion molecule ICAM-1 on alveolar macrophages and in serum in extrinsic allergic alveolitis.
ICAM-1 expression was studied on alveolar macrophages (AM) in extrinsic allergic alveolitis (EAA) using immunocytochemistry and cytometry. Compared to control donors, EAA patients had higher percentages of ICAM-1+ AM (75% vs. 30%) and the antigen density was more than sixfold higher (673 vs. 103 channels). Levels of soluble serum ICAM-1 were found increased in EAA patients with an average of 866.2 +/- 300 ng/ml versus 394.8 +/- 110 ng/ml in controls. These data are consistent with the concept that antigen contact upregulates ICAM-1 expression on AM in EAA followed by shedding and an increase in serum sICAM-1.
Pforte
A
A
Department of Internal Medicine, Klinikum Innenstadt, University of Munich, FRG.
Schiessler
A
A
Gais
P
P
von Kress
S
S
Beer
B
B
Riethmüller
G
G
Ziegler-Heitbrock
H W
HW
D000328:Adult; D000368:Aged; D000542:Alveolitis, Extrinsic Allergic / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D015815:Cell Adhesion Molecules / Q000032:analysis*; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D016676:Macrophages, Alveolar / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1