The effect of fenclofenac on the regeneration of lymphocytes in rats following total body irradiation.
The effect of fenclofenac on the regeneration of white blood cells in Sprague-Dawley rats following total body irradiation was investigated. The lowest dose of total body irradiation that would suppress the peripheral blood lymphocyte count by at least 50% was found to be 1.0 Gray in a preliminary experiment. Fenclofenac in a dose of 150 mg/kg/day retarded the regeneration of lymphocytes following total body irradiation of 1 Gray: this effect was also seen in the CD4, CD8 and B-lymphocyte subsets. This suppressive effect in vivo may be due to interference with replicative growth of white blood cells since previous in vitro experiments have shown suppression of lymphocyte growth at a stage later than activation in the cell cycle. The suppressive effects on leucocyte regeneration in vivo suggest that fenclofenac may have promise as an immunosuppressive drug with fewer serious side effects than the currently available immunosuppressive drugs.
Spiers
E M
EM
Department of Pathology, University of Dundee, U.K.
Watson
N T
NT
Beck
J S
JS
Chapman
I V
IV
Dettmar
P W
PW
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects; D016516:CD4-CD8 Ratio / Q000187:drug effects; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D010648:Phenylacetates / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014916:Whole-Body Irradiation
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010648:Phenylacetates; C012970:fenclofenac