title: Liquid chromatographic determination of magnolol in urine collected from volunteers after a single dose of saiboku-to, an oriental herbal medicine for bronchial asthma.
abstract: Saiboku-To is an anti-asthmatic herbal remedy which consists of ten herbal extracts. To investigate the clinical relationship between the effects and chemical components of Saiboku-To, a simple and sensitive high-performance liquid chromatographic method (HPLC) for determination of magnolol, one of the major urinary products, was developed. Organic solvent extraction of urinary magnolol was conducted by diatomaceous earth column rapid-flow fractionation using ethanol/dichloromethane (8/92, v/v). Recovery rates of magnolol were more than 99% with coefficient of variations less than 6% in the concentration range 9.7-970 ng mL-1. Subsequent HPLC determination of magnolol was achieved using a conventional silica-gel column, a mobile phase mixture of acetic acid/diethyl ether/n-hexane (0.2/17.0/82.8, v/v), and a UV-absorption detector set at 290 nm. Calibration was on the basis of peak height ratio between magnolol and flavone as an internal standard. The method was used to demonstrate excretion profiles of magnolol in healthy and asthmatic subjects following single administration of Saiboku-To.
authors.affiliation: Division of Clinical Pharmacology, Tokyo College of Pharmacy, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Homma
authors.forename: K
authors.initials: K
authors.lastname: Oka
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Niitsuma
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000188:drug therapy / Q000652:urine*; D001713:Biphenyl Compounds / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000627:therapeutic use; D006634:Histamine H1 Antagonists / Q000008:administration & dosage* / Q000627:therapeutic use; D006801:Humans; D017705:Lignans; D008297:Male; D020835:Medicine, Kampo; D008875:Middle Aged
chemical_list: D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D006634:Histamine H1 Antagonists; D017705:Lignans; C045086:saiboku-to; C005498:magnolol