title: Pollen-related allergy to peach and apple: an important role for profilin.
abstract: BACKGROUND
  Birch pollinosis is often accompanied by allergy to fruits such as peach and apple. Bet v I is of major importance as cross-reactive allergen for this combined allergy. We studied a group of patients with combined grass pollinosis and fruit allergy from an area virtually without birch trees.
  
  
  OBJECTIVE
  The aim of this study was to investigate the possible involvement of profilin and carbohydrate groups as cross-reactive structures in pollen and fruits.
  
  
  METHODS
  RAST inhibition was performed to measure cross-reactive IgE to pollen and fruits. The presence of IgE against profilin was determined in a RAST with purified grass profilin, and IgE against carbohydrate structures was determined in a RAST with proteinase K-digested grass pollen extract. The biologic activity of IgE in response to profilin was tested by in vitro histamine release and skin prick tests.
  
  
  RESULTS
  IgE against fruits was shown to be largely cross-reactive with grass pollen. The majority of the patients had IgE against profilin (12 of 16) and carbohydrate structures (9 of 10). Profilin was shown to have biologic activity, in both histamine release and skin prick tests.
  
  
  CONCLUSIONS
  Profilin is an important allergen for patients with combined grass pollen/fruit allergy in areas without birch trees.
authors.affiliation: Central Laboratory of The Netherlands, Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: R
authors.initials: R
authors.lastname: van Ree
authors.forename: M
authors.initials: M
authors.lastname: Fernández-Rivas
authors.forename: M
authors.initials: M
authors.lastname: Cuevas
authors.forename: M
authors.initials: M
authors.lastname: van Wijngaarden
authors.forename: R C
authors.initials: RC
authors.lastname: Aalberse
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D003285:Contractile Proteins; D003429:Cross Reactions; D005260:Female; D005638:Fruit / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology*; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D008297:Male; D008840:Microfilament Proteins / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology; D051304:Profilins; D011852:Radioallergosorbent Test
chemical_list: D000485:Allergens; D003285:Contractile Proteins; D008840:Microfilament Proteins; D010940:Plant Proteins; D051304:Profilins; D007073:Immunoglobulin E