FGF-2: apical ectodermal ridge growth signal for chick limb development.
The apical ectodermal ridge permits growth and elongation of amniote limb buds; removal causes rapid changes in mesodermal gene expression, patterned cell death, and truncation of the limb. Ectopic fibroblast growth factor (FGF)-2 supplied to the chick apical bud mesoderm after ridge removal will sustain normal gene expression and cell viability, and allow relatively normal limb development. A bioassay for FGFs demonstrated that FGF-2 was the only detectable FGF in chick limb bud extracts. By distribution and bioactivity, FGF-2 is the prime candidate for the chick limb bud apical ridge growth signal.
Fallon
J F
JF
Anatomy Department, University of Wisconsin, Madison 53706.
López
A
A
Ros
M A
MA
Savage
M P
MP
Olwin
B B
BB
Simandl
B K
BK
D000818:Animals; D001681:Biological Assay; D016923:Cell Death; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000235:genetics; D004475:Ectoderm / Q000737:chemistry / Q000502:physiology*; D005121:Extremities / Q000196:embryology*; D005346:Fibroblast Growth Factors / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D015870:Gene Expression; D005801:Genes, Homeobox; D018398:Homeodomain Proteins; D006801:Humans; D051957:MSX1 Transcription Factor; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D009132:Muscles / Q000166:cytology; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor; D011994:Recombinant Proteins; D014157:Transcription Factors; D005346:Fibroblast Growth Factors