title: Human CD8+ T lymphocyte clones specific for T cell receptor V beta families expressed on autologous CD4+ T cells.
abstract: CD8+ T cells control immune responses, and recent studies suggest that this regulation is, in part, specifically directed towards TCR structures expressed by CD4+ cells. To develop a system to study the role of the TCR in regulatory interactions, we isolated clones of CD4+ cells expressing identified TCR V beta chains. These CD4+ clones were used to stimulate and expand autologous CD8+ cells, which kill the inducing CD4+ clone as well as independently isolated autologous CD4+ clones sharing the same TCR V beta as the inducing cell but not CD4+ T cells expressing different V beta TCRs. This V beta-specific cytotoxicity is dependent on the state of activation of the target cells and is not inhibited by an anti-class I monoclonal antibody, W6/32. We envision that V beta-specific CD8+ T cells of this type may regulate immune responses by direct interaction with antigen-activated CD4+ cells.
authors.affiliation: Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
authors.forename: R
authors.initials: R
authors.lastname: Ware
authors.forename: H
authors.initials: H
authors.lastname: Jiang
authors.forename: N
authors.initials: N
authors.lastname: Braunstein
authors.forename: J
authors.initials: J
authors.lastname: Kent
authors.forename: E
authors.initials: E
authors.lastname: Wiener
authors.forename: B
authors.initials: B
authors.lastname: Pernis
authors.forename: L
authors.initials: L
authors.lastname: Chess
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008213:Lymphocyte Activation; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis / Q000276:immunology*; D016176:T-Lymphocyte Subsets
chemical_list: D011948:Receptors, Antigen, T-Cell