[The quantitative study of the areas of active rDNA location and of the other parameters of the interphase nucleolus detectable by silver staining during the cell differentiation of the rat trophoblast].
Different quantitative parameters of nucleolar silver staining have been studied in the cambial rat trophoblast cells on the 12th, 13th and 14th days of gestation. It has been shown that the number of Ag-positive granules in the nucleoli varied from 10 to 120. The number and the total area of silver stained granules in the nuclei increased progressively in the course of polyploidization, but was not doubled passing to the next ploidy level. Nevertheless, nucleolar area increased proportionally to the ploidy degree. The mean number and the total area of Ag-stained granules as well as the nucleolar area estimated for each ploidy level did not change significantly in the course of placenta development, suggesting an unchanged level of NOR activity at the studied stages of trophoblast cell differentiation. The data obtained on the interphase nucleoli differ from the data of the analysis of the metaphase Ag-NOR at the same period of placenta development, suggesting a diversity in the interphase and metaphase NOR organization. A proportion of cells with different number of nucleoli in the cambial rat trophoblast cells was maintained unchanged in the studied period of the placenta development, the majority (80-90%) of cells contained from 1 to 3 nucleoli. Such a proportion was similar in the cells of different levels of ploidy up to 16c. In this connection the association of NORs is suggested to be in relation with switching from the polyploid mitotic cycle to the endoreduplication leading to polyteny.
Zybina
T G
TG
Zybina
E V
EV
Shteĭn
G I
GI
Severova
E L
EL
Dyban
A P
AP
D000818:Animals; D002454:Cell Differentiation; D002466:Cell Nucleolus / Q000648:ultrastructure*; D004275:DNA, Ribosomal / Q000648:ultrastructure*; D005260:Female; D007399:Interphase; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D011003:Ploidies; D051381:Rats; D016622:Silver Staining; D013997:Time Factors; D014327:Trophoblasts / Q000648:ultrastructure*
D004275:DNA, Ribosomal
Kolichestvennoe issledovanie zon lokalizatsii aktivnosti rDNK i drugikh parametrov interfaznogo iadryshka, vyiavliaemykh metodom serebreniia v khode differentsirovki kletok trofoblasta krysy.