Use of an operon fusion to induce expression and crystallisation of a Bacillus thuringiensis delta-endotoxin encoded by a cryptic gene.
A delta-endotoxin gene previously cloned from Bacillus thuringiensis subsp. galleriae has been shown by a combination of restriction mapping and DNA sequence analysis to be a cryIIB clone; in common with other cryIIB genes it was found to lack a functional promoter. Addition of a promoter resulted in expression of the gene in Bacillus thuringiensis but did not result in the formation of the crystalline inclusions normally associated with such toxins. Inclusion formation was only observed when the gene was incorporated into an operon containing a gene known to be involved in the crystallisation of another delta-endotoxin.
Crickmore
N
N
Department of Biochemistry, University of Cambridge, UK.
Wheeler
V C
VC
Ellar
D J
DJ
D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D018833:Chaperonins; D003001:Cloning, Molecular; D003460:Crystallization; D004269:DNA, Bacterial; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics*; D005786:Gene Expression Regulation; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D009876:Operon; D011506:Proteins / Q000502:physiology; D013045:Species Specificity
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; D011506:Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D018833:Chaperonins