[Role of LFA-1/ICAM-1 in the activation of murine thymocytes stimulated by Con A].
The biological significance of the signals triggered by the interaction of cell surface expressed LFA-1 and ICAM-1 has been investigated in Con A stimulated murine thymocytes. Addition of anti-LFA-1 or/and anti-ICAM-1 McAb to the thymocyte culture in the presence of Con A, the cell proliferation was profoundly inhibited. Such inhibitory effect was more significant by the addition of anti-LFA-1 than by the addition of anti-ICAM-1 McAb. FACS analysis revealed that the proliferation of CD8+ T cells was more sensitive to the inhibitory effect of anti-LFA-1 McAb than did the proliferation of CD4+ T cells. The anti-LFA-1 McAb imposed inhibitory effect, however, could be reversed by the supplementation of IL-2 in the initiation of the culture. The proliferation of cultured Con A activated T blast cells could no longer be inhibited by the addition of anti-LFA-1 McAb. Furthermore, neither anti-LFA-1 nor anti-ICAM-1 McAb could block PMA+A23187 induced thymocyte proliferation. The results imply that the ligand-receptor pair of LFA-1-ICAM-1 molecules also takes part in the early events of Con A activated signal transduction pathway which leads to the proliferation of thymocytes.
Lu
L S
LS
Department of Immunology, Beijing Medical University.
Chen
W F
WF
D000818:Animals; D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002455:Cell Division; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D018799:Intercellular Adhesion Molecule-1; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015398:Signal Transduction; D013950:Thymus Gland / Q000166:cytology
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D003208:Concanavalin A; D018799:Intercellular Adhesion Molecule-1