Optimization of the loading limit for capillary zone electrophoresis of synthetic opioid and tachykinin peptides: a study of the interactions among the amount of peptide, resolution, saturation, injection volume and capillary diameter.
The upper loading limit in the capillary zone electrophoresis (CZE) of a mixture of fourteen synthetic opioid peptides and one tachykinin peptide was determined for capillaries of 50-, 75-, 100-, 130-, 150-, and 200-microns internal diameter (I.D.) to optimize the separation of these neuropeptides. The loading limit is an important parameter for preparative post-CZE studies. Electrophoretic resolution is illustrated for each capillary I.D. as the injection volume and amount of injected peptide increase. Loading limit is evaluated, based on the considerations of resolution, saturation, and current-stability. Among the capillaries studied, the capillary with 100 microns I.D. gives the optimal loading limit (39-78 pmol of each peptide).
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Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee, Memphis 38163.
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D004586:Electrophoresis; D004744:Enkephalin, Methionine / Q000032:analysis; D007202:Indicators and Reagents; D009294:Narcotics / Q000737:chemistry*; D009479:Neuropeptides / Q000737:chemistry*; D018847:Opioid Peptides / Q000737:chemistry; D015320:Tachykinins / Q000737:chemistry*
D007202:Indicators and Reagents; D009294:Narcotics; D009479:Neuropeptides; D018847:Opioid Peptides; D015320:Tachykinins; D004744:Enkephalin, Methionine