title: A refined guinea pig model for evaluating delayed-type hypersensitivity reactions caused by Q fever vaccines.
abstract: The previously established model for determining dermal granulomatous reactivity of Q fever vaccines uses footpad injections to sensitize Hartley guinea pigs. We describe a substantial refinement to that model by substituting an alternate injection route and replacing the model with the hairless Hartley guinea pig. The experimental design incorporates a matrix of various antigen and adjuvant combinations administered by subcutaneous (SC) and intradermal routes of administration. The two Coxiella burnetii antigens evaluated were phase-I whole-cell vaccine (WCV) and phase-I chloroform-methanol residue vaccine. The adjuvants used were complete Freund's (CFA), incomplete Freund's, and RIBI's De-Tox. Phase-I C. burnetii WCV in CFA administered SC provided the most efficacious regimen evaluated for sensitizing hairless guinea pigs. The establishment of this animal model allows evaluation of candidate Q fever vaccines in a more humane model than that previously available.
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21701.
authors.forename: D L
authors.initials: DL
authors.lastname: Ruble
authors.forename: J J
authors.initials: JJ
authors.lastname: Elliott
authors.forename: D M
authors.initials: DM
authors.lastname: Waag
authors.forename: G P
authors.initials: GP
authors.lastname: Jaax
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D002725:Chloroform; D016997:Coxiella burnetii / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007114:Immunization; D007700:Kinetics; D000432:Methanol; D011778:Q Fever; D012882:Skin Tests
chemical_list: D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D002725:Chloroform; D000432:Methanol