Characterization of the gene encoding dihydroflavonol 4-reductase in tomato.
A cDNA clone (DFR) encoding dihydroflavonol 4-reductase was identified from tomato hypocotyls. Nucleotide and amino acid sequence comparisons to Petunia hybrida, Antirrhinum majus and Zea mays DFR sequences confirmed that the cDNA encodes the structural DFR gene. In tomato, the DFR sequence appeared to be present as a single gene and mapped to a region on chromosome 2 near two loci affecting anthocyanin pigmentation, are and aw. DFR was expressed in both leaf and hypocotyl tissue. Sequencing data from two DFR cDNA clones indicated there are alternative polyadenylation sites on DFR.
Bongue-Bartelsman
M
M
Department of Vegetable Crops, University of California-Davis 95616-8746.
O'Neill
S D
SD
Tong
Y
Y
Yoder
J I
JI
D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis / Q000235:genetics; D017343:Genes, Plant; D008040:Genetic Linkage; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014675:Vegetables / Q000201:enzymology* / Q000235:genetics*
D007527:Isoenzymes; D004247:DNA; D000429:Alcohol Oxidoreductases; C056570:dihydroflavanol 4-reductase