PCNA independence of Ki-67 expression in HPV infection.
12 Infectious Wart lesions were stained using the streptavidin-biotin immunoperoxidase method for PCNA10, MIB-1 (Ki-67 equivalent antigen) and Human Papilloma Virus antigen to study the effect of HPV presence on epidermal proliferation. Using strict methods to avoid observer bias, Ki-67 antigen was found in a high proportion of nuclei in the suprabasal layers together with HPV antigens, in the absence of PCNA staining. This finding indicates that DNA synthesis related, Ki-67 antigen bearing structures can be raised in the human nucleus in the absence of induction of PCNA bearing structures, suggesting also structural independence between the antigen bearing molecules.
Boon
M E
ME
LCPL Laboratories, Leiden, The Netherlands.
Howard
C V
CV
van Velzen
D
D
D000951:Antigens, Neoplasm / Q000032:analysis; D004848:Epithelium / Q000382:microbiology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D027383:Papillomaviridae / Q000302:isolation & purification; D018809:Proliferating Cell Nuclear Antigen; D012867:Skin / Q000382:microbiology / Q000473:pathology; D014412:Tumor Virus Infections / Q000378:metabolism / Q000473:pathology*; D014860:Warts / Q000378:metabolism / Q000473:pathology*
D000951:Antigens, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen