Effects of pertussis and cholera toxin on the interferon-gamma stimulated immunocytochemical staining of ICAM-1 and inositol phosphate formation in a human renal carcinoma cell line.
We have recently shown that interferon-gamma (IFN-gamma) stimulated immunocytochemical staining of the intercellular adhesion molecule ICAM-1 may be dependent on inositol phosphate formation in the human renal carcinoma cell line CaKi-1. In the present study we investigated the possible role of GTP-binding proteins (G-proteins) during IFN-gamma signalling. Preincubation of CaKi-1 cells for 24 h with increasing amounts of pertussis toxin (PT) or cholera toxin (CT), two regulators of G-protein activity, inhibited IFN-gamma induced ICAM-1 staining. Preincubation with PT or CT for 24 h also inhibited IFN-gamma induced inositol 1-monophosphate (Ins 1-P), inositol 1,4 bisphosphate (Ins 1,4-P2) and inositol 1,4,5 trisphosphate (Ins 1,4,5-P3) formation. Our findings suggest that IFN-gamma induced ICAM-1 staining and inositol phosphate formation in CaKi-1 cells is dependent on a PT and CT sensitive signalling pathway. This may reflect a role for G-proteins in the coupling of IFN-gamma receptor activation and phospholipase C catalyzed phosphoinositide hydrolysis.
Hansen
A B
AB
Department of Pathology, Herlev Hospital, University of Copenhagen, Denmark.
Bouchelouche
P N
PN
Andersen
C B
CB
D002292:Carcinoma, Renal Cell / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000187:drug effects*; D002772:Cholera Toxin / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007295:Inositol Phosphates / Q000096:biosynthesis*; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000502:physiology*; D007680:Kidney Neoplasms / Q000276:immunology; D037342:Pertussis Toxin; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D015815:Cell Adhesion Molecules; D007295:Inositol Phosphates; D010566:Virulence Factors, Bordetella; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma; D002772:Cholera Toxin; D037342:Pertussis Toxin