IFN-gamma induces IL-12 mRNA expression by a murine macrophage cell line, J774.
Interleukin (IL)-12 is a logical candidate for participation in the differentiation of T helper (Th) 1 cells. IL-12 is produced by macrophages and B cells and induces the production of interferon (IFN)-gamma from Th1 cells and NK cells. In this study, we show that the IFN-gamma itself is capable of inducing IL-12 mRNA expression in murine macrophage cell line J774. The mRNA was apparent by 12h after IFN-gamma treatment, although maximal induction required 36 to 48h. Furthermore, we investigated the signal transduction mechanism responsible for the mRNA expression. The IFN-gamma-inducible IL-12 mRNA was blocked by two protein tyrosine kinase (PTK) inhibitors, genistein and herbimycin A, suggesting that PTK is involved in the IFN-gamma-inducible IL-12 mRNA expression.
Yoshida
A
A
Department of Microbiology and Immunology, Hamamatsu University School of Medicine, Japan.
Koide
Y
Y
Uchijima
M
M
Yoshida
T O
TO
D000818:Animals; D001483:Base Sequence; D016227:Benzoquinones; D002460:Cell Line; D017931:DNA Primers; D015870:Gene Expression / Q000187:drug effects*; D019833:Genistein; D007371:Interferon-gamma / Q000494:pharmacology*; D018664:Interleukin-12; D007378:Interleukins / Q000096:biosynthesis*; D007529:Isoflavones / Q000494:pharmacology; D007700:Kinetics; D047029:Lactams, Macrocyclic; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011809:Quinones / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D011994:Recombinant Proteins; D017828:Rifabutin / Q000031:analogs & derivatives
D016227:Benzoquinones; D017931:DNA Primers; D007378:Interleukins; D007529:Isoflavones; D047029:Lactams, Macrocyclic; D011809:Quinones; D012333:RNA, Messenger; D011994:Recombinant Proteins; D018664:Interleukin-12; D017828:Rifabutin; C020754:herbimycin; D007371:Interferon-gamma; D019833:Genistein; D011505:Protein-Tyrosine Kinases