[Studies on the gap junctional intercellular communication of human nasopharyngeal carcinoma cells and effect of RII].
To evaluate the effects of gap junctional intercellular communication (GJIC) on the growth, invasion and metastasis of tumor, human nasopharyngeal carcinoma (HNPC) parent cell line (CNE-2Z) and its variants (L2, H2, L4) with different invasive and metastatic potential were examined in vitro. Only a few intermediate junction (IJ) but no gap junction (GJ) structures were observed under EM. The parent line cells showed marked GJIC, while its variants lacked this function by SLDT technique. It was further shown that L2 cell line (variant with high invasive potential) had lower concentration of cytosolic free calcium ([Ca2+]i) compared to H2, L4 cell lines (variants with medium and low invasive potential, respectively). It reflected that some correlation may exist between [Ca2+]i level and the invasive potential of HNPC cell lines. The effect of RII on GJIC of HNPC was also investigated. After 3-7 ds of RII (0.0001 mol/L) treatment, there was no change in the number of GJs. The GJIC function of CNE-2Z weakened and then disappeared finally with prolonged RII treatment. The level of [Ca2+]i in HNPC cells apparently fell after 6h of RII treatment, and rose to original level with persistent RII treatment. Whether the fluctuating of [Ca2+]i level relates the inhibitory effect of RII treatment. Treatment on the growth and invasion needs to be further studied.
Han
L Q
LQ
Department of Pathology, Chinese Academy of Medical Sciences, Beijing.
Gao
J
J
Dong
H Y
HY
D002118:Calcium / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002450:Cell Communication / Q000187:drug effects*; D017629:Gap Junctions / Q000502:physiology*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D014212:Tretinoin; D002118:Calcium