Purification and characterization of distinct type of mannose-sensitive fimbriae from Salmonella typhimurium.
The fimbriae (E4) of a virulent strain of Salmonella typhimurium were purified by ion exchange chromatography in an FPLC system. They had a channelled appearance under transmission electron microscope and showed a major structural subunit of 17-kDa on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The purified fimbriae were found to agglutinate guinea pig erythrocytes, but this effect was inhibited in presence of D-mannose. Immune sera raised against the Mono-Q purified fimbriae (E4) showed cross-reactivity with the type-1 fimbriae (F1) composed of 21-kDa fimbrin subunit, purified by a different method from the same strain.
Ghosh
S
S
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Mittal
A
A
Ganguly
N K
NK
D000818:Animals; D000907:Antibodies, Bacterial; D003429:Cross Reactions; D004305:Dose-Response Relationship, Drug; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D005947:Glucose / Q000494:pharmacology; D006168:Guinea Pigs; D006384:Hemagglutination / Q000187:drug effects*; D008353:Mannitol / Q000494:pharmacology; D008358:Mannose / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D013045:Species Specificity
D000907:Antibodies, Bacterial; D008353:Mannitol; D005947:Glucose; D008358:Mannose