Assignment of the norepinephrine transporter protein (NET1) locus to chromosome 16.
The norepinephrine transporter protein (NET) is the presynaptic reuptake site for norepinephrine and a site of action for several drugs with CNS effects, some of which are therapeutically useful and some of which are drugs of abuse. We used PCR with a somatic cell hybrid panel to obtain a provisional assignment to chromosome 16. We then typed a genetic polymorphism at the NET1 locus in three large multigenerational families and used linkage analysis to confirm the preliminary assignment and to refine the localization to 16q, near the HP locus. Finally, we typed the NET1 RFLP on the CEPH families and the additional linkage data localized NET1 to 16q13-q21, flanked by D16S71 (centromerically) and HP (telomerically).
Gelernter
J
J
West Haven VA Medical Center, Connecticut 06516.
Kruger
S
S
Pakstis
A J
AJ
Pacholczyk
T
T
Sparkes
R S
RS
Kidd
K K
KK
Amara
S
S
D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D018076:DNA, Complementary; D015252:Deoxyribonucleases, Type II Site-Specific; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D009638:Norepinephrine / Q000378:metabolism; D050484:Norepinephrine Plasma Membrane Transport Proteins; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D027981:Symporters
D002352:Carrier Proteins; D018076:DNA, Complementary; D005819:Genetic Markers; D050484:Norepinephrine Plasma Membrane Transport Proteins; C493227:SLC6A2 protein, human; D027981:Symporters; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases; D009638:Norepinephrine