title: Ly-49A, a receptor for H-2Dd, has a functional carbohydrate recognition domain.
abstract: Ly-49A+ murine natural killer (NK) cells cannot lyse target cells that express H-2Dd. We demonstrate a functional requirement for carbohydrate recognition by Ly-49A. Treatment of H-2Dd+ target cells with tunicamycin prevents their binding to Ly-49A+ cells and renders them susceptible to lysis by Ly-49A+ NK cells. Fucoidan, a sulfated polysaccharide, binds to Ly-49A in a calcium-dependent manner, and this binding is inhibited by monosaccharides, particularly sulfated hexoses. The inactivation of Ly-49A+ NK cells by H-2Dd+ target cells is reversed in the presence of glucose 6-SO4. These results indicate that Ly-49A has a functional carbohydrate recognition domain and that target expression of carbohydrates alters their susceptibility to natural killing.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143.
authors.forename: B F
authors.initials: BF
authors.lastname: Daniels
authors.forename: M C
authors.initials: MC
authors.lastname: Nakamura
authors.forename: S D
authors.initials: SD
authors.lastname: Rosen
authors.forename: W M
authors.initials: WM
authors.lastname: Yokoyama
authors.forename: W E
authors.initials: WE
authors.lastname: Seaman
mesh_terms: D000818:Animals; D000950:Antigens, Ly; D016466:CHO Cells; D002118:Calcium / Q000494:pharmacology; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D002460:Cell Line; D006224:Cricetinae; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006183:H-2 Antigens / Q000378:metabolism*; D064532:Histocompatibility Antigen H-2D; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism*; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000378:metabolism / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D055630:Receptors, NK Cell Lectin-Like; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D000950:Antigens, Ly; D002241:Carbohydrates; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D011134:Polysaccharides; D011971:Receptors, Immunologic; D055630:Receptors, NK Cell Lectin-Like; D014415:Tunicamycin; C007789:fucoidan; D005947:Glucose; D002118:Calcium