title: [Effect of prednisolon, vincristine and mitoxantrone on glucocorticoid receptor in HL-60 cell line].
abstract: The effects of prednisolon, vincristine and Mitoxantrone on glucocorticoid receptor (GCR) content and affinity were examined in HL-60 cell line with a whole-cell assay in vitro. The fall in GCR number was observed following a short time exposure to prednisolon, vincristine or Mitoxantrone alone. The significant decrease occurred in a dose-dependent (10(-8)-10(-6) mol/L) and time-dependent manner. The treatment of HL-60 cells with 10(-6) mol/L led to a decrease in the affinity of whole cell GCR for [3H]Dex, but vincristine had no that effect. Synergia in GCR content was found during the treatment of HL-60 cells with prednisolon plus Mitoxantrone or prednisolon plus vincristine. The significance of these effects is discussed.
authors.forename: S
authors.initials: S
authors.lastname: Cai
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Ye
authors.forename: F
authors.initials: F
authors.lastname: Yu
mesh_terms: D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D008942:Mitoxantrone / Q000494:pharmacology*; D011239:Prednisolone / Q000494:pharmacology*; D011965:Receptors, Glucocorticoid / Q000187:drug effects*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014750:Vincristine / Q000494:pharmacology*
chemical_list: D011965:Receptors, Glucocorticoid; D014750:Vincristine; D011239:Prednisolone; D008942:Mitoxantrone