Convulsant doses of cocaine alter immediate early gene and opioid peptide expression in rat limbic forebrain.
Rats were treated with escalating doses of cocaine until they experienced a convulsion and were euthanized 1 or 3 h after the last injection. Quantitative in situ hybridization histochemistry revealed that c-fos and zif/268 mRNAs were induced at 1 h in many limbic structures and declined 3 h after cocaine-induced convulsions. Preprodynorphin and preproenkephalin signals increased in many of the same structures 3 h, but not 1 h, after cocaine-induced convulsions.
Helton
T E
TE
Department of Anatomy and Cell Biology, East Carolina University School of Medicine, Greenville, NC 27858-4354.
Daunais
J B
JB
McGinty
J F
JF
D000818:Animals; D003042:Cocaine / Q000494:pharmacology* / Q000633:toxicity; D003292:Convulsants / Q000494:pharmacology* / Q000633:toxicity; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D004399:Dynorphins / Q000096:biosynthesis / Q000235:genetics*; D051766:Early Growth Response Protein 1; D004745:Enkephalins / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D017874:Immediate-Early Proteins; D017403:In Situ Hybridization; D007696:Kindling, Neurologic; D008032:Limbic System / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D012640:Seizures / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
D003292:Convulsants; D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D004745:Enkephalins; D017874:Immediate-Early Proteins; D009419:Nerve Tissue Proteins; D011498:Protein Precursors; D016760:Proto-Oncogene Proteins c-fos; D014157:Transcription Factors; C036448:pre-prodynorphin; D004399:Dynorphins; C033660:preproenkephalin; D003042:Cocaine