Calbindin, tyrosine hydroxylase and opioid-like immunoreactivity in the intestinal nerve of Remak of the domestic fowl.
Four classes of neurons were identified in both juxta-jejunal and juxta-rectal ganglia of Remak's nerve of the domestic fowl using double-labeling immunohistochemistry. Neurons immunoreactive (IR) for tyrosine hydroxylase (TH) formed a mutually exclusive subpopulation from neurons displaying calbindin (CaBP)-IR. Between 48-72% of juxta-jejunal neurons labeled for TH whereas 36-57% of juxta-rectal neurons displayed TH-IR. CaBP-IR was present in 18-40% of juxta-jejunal neurons; this increased to 31-46% in juxta-rectal neurons. The majority of CaBP-IR neurons (78-85%) also displayed opioid (beta-EP)-IR. Within each ganglion a small percentage of neurons (4-18%) were non-IR with any of the three antibodies. This is the first report of an immunohistochemically identified subpopulation of non-catecholaminergic neurons within the juxta-jejunal ganglia of Remak's nerve. It is proposed that these perikarya are a major source of the CaBP-IR and opioid-IR nerve fibers found in the chicken gut.
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Department of Anatomy and Histology, Flinders University, Bedford Park, South Australia.
D000818:Animals; D064026:Calbindins; D002645:Chickens; D004723:Endorphins / Q000276:immunology / Q000378:metabolism*; D005725:Ganglia, Autonomic / Q000166:cytology; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007422:Intestines / Q000294:innervation*; D008297:Male; D064030:S100 Calcium Binding Protein G / Q000276:immunology / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology / Q000378:metabolism*
D064026:Calbindins; D004723:Endorphins; D064030:S100 Calcium Binding Protein G; D014446:Tyrosine 3-Monooxygenase