High-performance liquid chromatographic analysis of human erythrocyte oxysterols as delta 4-3-ketone derivatives.
Following oxidation by cholesterol oxidase human erythrocyte oxysterols were analyzed as the corresponding hydroxy-delta 4-3-ketones by HPLC with effluent monitoring at 235 nm. Variable amounts of epimeric cholest-5-ene-3 beta,7-diols, 3 beta-hydroxycholest-5-en-7-one, cholest-5-ene-3 beta,19-diol, cholest-5-ene-3 beta,20-diol, cholest-5-ene-3 beta,25-diol and cholest-5-ene-3 beta,26-diol were detected in erythrocyte membranes of patients with sickle cell anemia or sickle cell trait. Only 3 beta-hydroxycholest-5-en-7-one was detected in erythrocyte membranes of healthy donors.
Teng
J I
JI
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555.
Smith
L L
LL
D000755:Anemia, Sickle Cell / Q000097:blood; D002783:Cholestenones / Q000097:blood*; D002789:Cholesterol Oxidase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D007659:Ketones; D012805:Sickle Cell Trait / Q000097:blood
D002783:Cholestenones; D007659:Ketones; D002789:Cholesterol Oxidase