Changes in homeobox-containing gene expression during ectopic bone formation induced by bone morphogenetic protein.
Expression of homeobox genes in relation to ectopic bone formation induced by bone morphogenetic protein (BMP) was investigated. Oligonucleotide primers corresponding to highly conserved regions of Hox cluster and Msx genes were designed to detect homeobox sequences by means of the polymerase chain reaction (PCR). Nine rat homologues of Hox cluster genes and two Msx genes were discovered in the BMP-implanted tissue, at earlier stage and later cartilage and bone formation stage, respectively. The PCR study provided evidence of dynamic changes in BMP-induced homeobox gene expression.
Iimura
T
T
Department of Biochemistry, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Oida
S
S
Takeda
K
K
Maruoka
Y
Y
Sasaki
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001846:Bone Development / Q000187:drug effects / Q000502:physiology*; D019485:Bone Morphogenetic Proteins; D002356:Cartilage / Q000187:drug effects / Q000473:pathology; D002828:Choristoma / Q000139:chemically induced; D003001:Cloning, Molecular; D003094:Collagen / Q000032:analysis; D017124:Conserved Sequence; D017931:DNA Primers; D004343:Drug Implants; D005786:Gene Expression Regulation / Q000187:drug effects*; D005801:Genes, Homeobox; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012689:Sequence Homology, Nucleic Acid
D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D004343:Drug Implants; D011506:Proteins; D003094:Collagen