Chaperonin GroE and ADP facilitate the folding of various proteins and protect against heat inactivation.
In the presence of ADP, the molecular chaperones GroEL and GroES from Escherichia coli not only facilitated the refolding of various proteins, but also prevented their irreversible heat inactivation in vitro. Without nucleotides the refolding reactions were arrested by GroEL. Addition of GroES and ADP to the reaction mixture initiated the refolding reactions and the enzyme activities were regained efficiently. The presence of GroE (GroEL and GroES) and ADP also protected against heat inactivation of native enzymes at various temperatures. These findings suggest that in the presence of GroES, nucleotide binding is an important event in the mechanism of GroEL-facilitated protein folding.
Kawata
Y
Y
Department of Biotechnology, Faculty of Engineering, Tottori University, Japan.
Nosaka
K
K
Hongo
K
K
Mizobata
T
T
Nagai
J
J
D000244:Adenosine Diphosphate / Q000378:metabolism* / Q000494:pharmacology; D001236:Aspergillus oryzae / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D018835:Chaperonin 10; D018834:Chaperonin 60; D004795:Enzyme Stability; D004798:Enzymes / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism*; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000737:chemistry; D019791:Guanidine; D006146:Guanidines; D006360:Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D008291:Malate Dehydrogenase / Q000737:chemistry; D011489:Protein Denaturation; D017510:Protein Folding; D013210:Staphylococcus / Q000201:enzymology; D013816:Thermodynamics; D013824:Thermus / Q000201:enzymology; D000516:alpha-Amylases / Q000737:chemistry
D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D004798:Enzymes; D006146:Guanidines; D006360:Heat-Shock Proteins; D000244:Adenosine Diphosphate; D005948:Glucose Dehydrogenases; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D042843:Glucose 1-Dehydrogenase; D000516:alpha-Amylases; D019791:Guanidine