title: Differential regulation of tyrosine hydroxylase protein and activity in rabbit sympathetic neurones after long-term cold exposure: altered responses in ageing.
abstract: The aim of this study was to investigate the response of sympathetic neurones to prolonged neural stimulation, using cold exposure as a non-invasive experimental paradigm. We examined the effects of prolonged (8 days and 4 wk) cold exposure on tyrosine hydroxylase (TH) protein and activity and neuropeptide Y (NPY) levels in sympathetic neurones of the superior cervical ganglion (SCG), together with NPY levels in the ear artery from young and aged rabbits. The main findings were as follows. In young rabbits, TH levels and TH activity were differentially regulated in response to prolonged cold exposure. TH levels rose whilst TH activity tended to decline. Decentralization of SCG from young animals before cold exposure abolished the rise in TH levels. TH activity in SCG from young rabbits was reduced by decentralization whilst cold exposure resulted in an increase in TH activity. Thus, TH activity was induced in the SCG in the absence of pre-ganglionic input, demonstrating a non-synaptic regulatory mechanism. In old rabbits, cold-induced changes were either delayed or failed to occur, indicating that the responses of sympathetic neurones to cold stress are impaired in old age.
authors.affiliation: Department of Anatomy and Developmental Biology, Royal Free Hospital School of Medicine, London, UK.
authors.forename: T
authors.initials: T
authors.lastname: Andrews
authors.forename: J
authors.initials: J
authors.lastname: Lincoln
authors.forename: P
authors.initials: P
authors.lastname: Milner
authors.forename: G
authors.initials: G
authors.lastname: Burnstock
authors.forename: T
authors.initials: T
authors.lastname: Cowen
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001158:Arteries; D003080:Cold Temperature; D004797:Enzyme-Linked Immunosorbent Assay; D005728:Ganglia, Sympathetic / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009478:Neuropeptide Y / Q000097:blood / Q000378:metabolism; D011817:Rabbits; D013312:Stress, Physiological / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
chemical_list: D009478:Neuropeptide Y; D014446:Tyrosine 3-Monooxygenase