Inhibition of interferon-gamma-induced major histocompatibility complex class I expression by certain oligodeoxynucleotides.
We report that certain oligonucleotides are capable of inhibiting cell surface induction of the major histocompatibility complex class I (MHC-I) proteins by interferon-gamma in K562 cells. The inhibition by oligodeoxy-nucleotide I 5' GGG GTT GGT TGT GTT GGG TGT TGT GT-RNH2 is dose-dependent, with an EC50 24 hr after dosing of approximately 4 microM for 800 U/ml interferon-gamma. The reverse complement II 5' AC ACA ACA CCC AAC ACA ACC AAC CCC-RNH2 did not show activity. Oligodeoxynucleotide I inhibits induction of MHC-I by interferon-gamma, but does not inhibit induction by either interferon-alpha or interferon-beta. Four other oligodeoxynucleotides were also evaluated, and three showed activity against interferon-gamma at 25 microM.
Ramanathan
M
M
University of California, San Francisco, California.
Lantz
M
M
MacGregor
R D
RD
Huey
B
B
Tam
S
S
Li
Y
Y
Garovoy
M R
MR
Hunt
C A
CA
D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000037:antagonists & inhibitors*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011990:Receptors, Transferrin / Q000378:metabolism
D015815:Cell Adhesion Molecules; D015395:Histocompatibility Antigens Class I; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma