Cloning and molecular analysis of the Salmonella enterica ansP gene, encoding an L-asparagine permease.
A gene (ansP), which encodes an L-asparagine permease, has been isolated from a cosmid library of Salmonella enterica during screening for recombinant clones which encode L-asparaginase. Nucleotide sequence analysis reveals that the gene product is a polypeptide of 497 amino acid residues, containing 12 putative transmembrane segments. The calculated molecular mass is 54 kDa, although maxicell analysis by SDS-PAGE gave an apparent molecular mass of 37 kDa. Comparison of the deduced amino acid sequence with sequence databases showed significant homology with a family of basic and aromatic amino acid permeases. Strains containing the cloned ansP gene demonstrated a many-fold increase in L-asparagine uptake in comparison with control strains.
Jennings
M P
MP
Division of Science and Technology, Griffith University, Nathan, Queensland, Australia.
Anderson
J K
JK
Beacham
I R
IR
D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems; D001216:Asparagine / Q000378:metabolism; D001426:Bacterial Proteins; D001483:Base Sequence; D001692:Biological Transport; D003001:Cloning, Molecular; D003360:Cosmids; D004926:Escherichia coli; D015723:Gene Library; D005798:Genes, Bacterial; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D012475:Salmonella / Q000201:enzymology* / Q000235:genetics*
D026905:Amino Acid Transport Systems; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; C092723:asparagine permease protein, Salmonella enterica; D001216:Asparagine