Differentiation of Rickettsia japonica by restriction endonuclease fragment length polymorphism using products of polymerase chain reaction amplification with Rickettsia rickettsii 190-kilodalton surface antigen gene primers.
Restriction fragment length polymorphism of polymerase chain reaction (PCR) amplification products differentiated Rickettsia japonica, a causative agent of Oriental spotted fever, from other spotted fever group (SFG) rickettsiae. Primer pair Rr190. 70p and Rr190. 602n of R. rickettsii 190-kDa antigen gene sequence primed genomic DNAs obtained from R. japonica, type strain YH and strains NT, NK, YKI, and TKN. The products were cleaved by PstI but not by AfaI restriction endonuclease. The PstI digestion pattern of PCR-products amplified from all strains of R. japonica was identical and easily differentiated from that of other SFG rickettsiae. The present study demonstrated a genotypic difference between R. japonica and other pathogenic SFG rickettsiae.
Yan
Y
Y
Department of Virology, School of Medicine, University of Tokushima, Japan.
Uchiyama
T
T
Uchida
T
T
D000818:Animals; D000942:Antigens, Bacterial; D017931:DNA Primers; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; D004587:Electrophoresis, Agar Gel; D016680:Genome, Bacterial; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000175:diagnosis; D012284:Rickettsia rickettsii / Q000235:genetics / Q000302:isolation & purification*; D014709:Vero Cells
D000942:Antigens, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes