title: Lymphocyte subsets, sIL2-R and sICAM-1 in blood during allergen challenge tests in asthmatic children.
abstract: In order to study cell activation in peripheral blood on bronchial allergen provocation up to 24 h, we investigated 32 asthmatic children, sensitive to house-dust mites. Six healthy young adult volunteers served as controls. Lymphocyte subsets (CD3, CD19, CD4, CD8) and activation markers (CD25-T, HLADR-T, CD23) in peripheral blood as well as soluble IL2-R and soluble ICAM-1 in serum were evaluated. In terms of clinical reaction, 23 children exhibited a DAR, 6 an EAR, 6 a LAR and 3 children did not show a bronchoconstrictor response to allergen challenge with house-dust mite extract (NAR). In comparison to controls, asthmatic children showed a significantly higher expression of CD23 on B-lymphocytes (p < 0.05). Other subsets were in the same range in both groups. After provocation there was a significant increase of CD4/CD8-ratio only in asthmatic children. Serum levels of sIL2-R were significantly higher in asthmatic children compared to controls at baseline as well as at 12 and 24 h after provocation, without variation during observation period. No differences were noted for sICAM-1. Our results confirm the hypothesis that lymphocytes, as important cells in regulation of allergic immune response, are recruited into peripheral blood under allergen challenge conditions in sensitized asthmatic children.
authors.affiliation: University Childrens Hospital (KAVH), Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schmitt
authors.forename: B
authors.initials: B
authors.lastname: Niggemann
authors.forename: I
authors.initials: I
authors.lastname: Kleinau
authors.forename: S
authors.initials: S
authors.lastname: Nasert
authors.forename: A
authors.initials: A
authors.lastname: Kapp
authors.forename: U
authors.initials: U
authors.lastname: Wahn
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D001249:Asthma / Q000276:immunology*; D016516:CD4-CD8 Ratio; D015815:Cell Adhesion Molecules / Q000097:blood*; D002648:Child; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D015375:Receptors, Interleukin-2 / Q000032:analysis*
chemical_list: D000485:Allergens; D015815:Cell Adhesion Molecules; D015375:Receptors, Interleukin-2; D018799:Intercellular Adhesion Molecule-1