title: Transformation of Chlorobium limicola by a plasmid that confers the ability to utilize thiosulfate.
abstract: A modified transformation method for Chlorobium limicola confirms the role of an endogenous plasmid in thiosulfate metabolism. The plasmid was present in two forma specialis thiosulfatophilum (Tio+) strains and absent in one non-thiosulfate-utilizing (Tio-) strain. The plasmid (size, 14 kb) was transferred by transformation from Tio- to Tio+. The chromosomal restriction patterns, analyzed by pulsed-field gel electrophoresis, were used to distinguish between the different strains.
authors.affiliation: Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Méndez-Alvarez
authors.forename: V
authors.initials: V
authors.lastname: Pavón
authors.forename: I
authors.initials: I
authors.lastname: Esteve
authors.forename: R
authors.initials: R
authors.lastname: Guerrero
authors.forename: N
authors.initials: N
authors.lastname: Gaju
mesh_terms: D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D010957:Plasmids / Q000235:genetics*; D013885:Thiosulfates / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D013885:Thiosulfates; D015252:Deoxyribonucleases, Type II Site-Specific