Cytochrome c-551 of the thermophilic bacterium PS3, DNA sequence and analysis of the mature cytochrome.
The structural gene for cytochrome c-551 was isolated from genomic DNA of the thermophilic bacterium PS3. The amino acid sequence of cytochrome c-551 as deduced from the DNA sequence consists of 111 amino acid residues and contains one heme c-binding site (-CASCH-) located approximately in the middle of the polypeptide. The N-terminus of isolated cytochrome c-551 was blocked, but treatment with Rhizopus lipase and molecular weight measurement of the mature and lipase-treated forms by ion spray mass spectroscopy suggest that the mature c-551 may have 93 or 94 amino acid residues with a diacylated glycerol-cysteine at the N-terminal region. The first 17 or 18 amino acid residues in the N-terminal region of the nascent polypeptide, rich in hydrophobic and basic amino acid residues, may be a signal peptide to translocate the major portion of cytochrome c-551 to the extracellular surface and to be processed. Similarity of amino acid sequence of this protein is discussed in relation to other c-type cytochromes of bacilli as well as bacterial small cytochromes c such as Pseudomonas aeruginosa cytochrome c-551 and cytochrome c6 of cyanobacteria.
Fujiwara
Y
Y
Department of Biochemistry, Jichi Medical School, Tochigi-ken, Japan.
Oka
M
M
Hamamoto
T
T
Sone
N
N
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000032:analysis / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D008049:Lipase; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D013824:Thermus / Q000201:enzymology*
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D004247:DNA; C017167:cytochrome C(551); D008049:Lipase