Mitogenic effects of platelet-derived growth factor, fibroblast growth factor, transforming growth factor-beta, and heparin-binding serum factor for adult mouse Schwann cells.
Mitogenic effects of fetal calf serum (FCS), platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor-beta (TGF-beta), and forskolin to adult mouse Schwann cells were examined by bromodeoxyuridine (BrdU) incorporation and double immunofluorescence for S100 and BrdU. PDGF-BB, basic FGF, and TGF-beta 1 and beta 2 were all mitogenic for Schwann cells in media containing FCS. Forskolin suppressed the mitogenic activity of these factors. In serum-free media, PDGF-BB and bFGF were also mitogenic, but TGF-beta 1 and beta 2 were not. Heparin-binding fractions of FCS obtained by heparin-Sepharose chromatography synergized with TGF-beta 1 and beta 2 to produce a mitogenic response. Since PDGF-BB, acidic FGF, and basic FGF were not detected in these fractions by immunoabsorption and immunoblot assays, the presence of unidentified heparin-binding molecules in FCS bioactive for adult mouse Schwann cells is suggested.
Watabe
K
K
Division of Neuropathology, Jikei University School of Medicine, Tokyo, Japan.
Fukuda
T
T
Tanaka
J
J
Toyohara
K
K
Sakai
O
O
D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D016895:Culture Media, Serum-Free; D004357:Drug Synergism; D005312:Fetal Blood / Q000737:chemistry; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology; D006493:Heparin / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008934:Mitogens / Q000097:blood; D010525:Peripheral Nerves / Q000166:cytology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D001685:Biological Factors; D001798:Blood Proteins; D016895:Culture Media, Serum-Free; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D005576:Colforsin; D005346:Fibroblast Growth Factors; D006493:Heparin