title: Hydroxyl radical generation by coal mine dust: possible implication to coal workers' pneumoconiosis (CWP).
abstract: Occupational exposure to coal mine dust causes coal workers' pneumoconiosis (CWP) and other pulmonary diseases by mechanisms that remain unclear. Because the hydroxyl radicals (.OH) may play an important role in the pathogenesis of CWP, we studied the potential role of bituminous coal mine dust samples for catalyzing the generation of .OH from hydrogen peroxide (H2O2). These coal mine dusts evaluated represented two geographic areas with diversity in CWP prevalence. Electron spin resonance (ESR), with the aid of spin trapping techniques, was used to measure the .OH radical generation. Bituminous coal mine dusts representing the Pittsburgh seam in the eastern United States and Blind Canyon seam in the mid-western United States were used together with a standard coal dust obtained from the National Institute of Standards and Technology, Gaithersburg, MD. All the coal mine dust samples generated varying levels of .OH radicals from H2O2 in the presence of a .OH spin trap 5,5-dimethyl-l-pyrroline-N-oxide (DMPO). .OH radical generation by the coal from H2O2 was effectively inhibited by deferoxamine and catalase, but only partially inhibited by superoxide dismutase. Metal chelators DETAPAC and EDTA enhanced the radical generation. These results indicated that the Fenton reaction is predominantly involved in the generation of .OH radicals from H2O2. The .OH-generating potential of all the coal dusts showed a positive correlation with the surface iron content of coal mine dusts. In addition, the potential to induce lipid peroxidation by the coal samples exhibited a good correlation with the available surface iron. Based on the results presented here, we propose that higher concentrations of surface iron in coal mine dust may be involved in the generation of increased levels of .OH radicals and may play an important role in the development of CWP in different coal mining areas.
authors.affiliation: National Institute for Occupational Safety and Health, Division of Respiratory Disease Studies, Morgantown, WV.
authors.forename: N S
authors.initials: NS
authors.lastname: Dalal
authors.forename: J
authors.initials: J
authors.lastname: Newman
authors.forename: D
authors.initials: D
authors.lastname: Pack
authors.forename: S
authors.initials: S
authors.lastname: Leonard
authors.forename: V
authors.initials: V
authors.lastname: Vallyathan
mesh_terms: D002614:Chelating Agents; D003031:Coal; D003032:Coal Mining; D003497:Cyclic N-Oxides; D004391:Dust; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry*; D015227:Lipid Peroxidation; D016273:Occupational Exposure; D011009:Pneumoconiosis / Q000209:etiology*; D013113:Spin Labels
chemical_list: D002614:Chelating Agents; D003031:Coal; D003497:Cyclic N-Oxides; D004391:Dust; D016166:Free Radical Scavengers; D013113:Spin Labels; D017665:Hydroxyl Radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D006861:Hydrogen Peroxide