[The HPLC analysis of chiral beta-receptor blockers].
Investigations on direct separation by RP-HPLC of selected enantiomeric beta-adrenergic active agents are described. R- and S-1-phenylethylisocyanate (PEIC) as well as (1S)-(+)-campher-10-sulfonylchloride (CSC) are used for the derivatization of the compounds. Correlations between chromatographic data and various influences (pH, temperature, reaction time, concentration of reagens a.o.) have been discussed. The behaviour of derivatized and underivatized analytes on RP-columns is described in dependence on buffer concentration of the mobile phase. The investigated beta-adrenergic agents were partly separated after derivatization with PEIC, whereas this was not possible with CSC as derivatization reagent.
Jira
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T
Institut für Arzneimittelkontrolle, Ernst-Moritz-Arndt-Universität Greifswald.
Breyer
C
C
D000319:Adrenergic beta-Antagonists / Q000737:chemistry* / Q000302:isolation & purification; D002164:Camphor / Q000031:analogs & derivatives / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D017879:Isothiocyanates / Q000737:chemistry; D013237:Stereoisomerism; D013450:Sulfones / Q000737:chemistry; D013696:Temperature
D000319:Adrenergic beta-Antagonists; D007202:Indicators and Reagents; D017879:Isothiocyanates; D013450:Sulfones; C085561:camphor-10-sulfonyl chloride; C058305:phenethyl isothiocyanate; D002164:Camphor
Zur HPLC-Analytik chiraler beta-Rezeptorenblocker.