Designation by restriction fragment length polymorphism of major histocompatibility complex class IV haplotypes in meat-type chickens.
Major histocompatibility complex (MHC) class IV haplotypes were identified in a population of meat-type chickens by restriction fragment length polymorphism (RFLP) analysis. Fourteen different haplotypes were designated on the basis of restriction patterns obtained from Southern blots of PvuII- or BglII-digested DNA, hybridized with the MHC class IV cDNA probe bg32.1. Digestion with each restriction enzyme yielded the same level of polymorphism among individuals. For each haplotype, 4-10 restriction fragments ranging from 0.8 to 8 kb were observed. Such a designation of meat-type chicken MHC class IV haplotypes enables a rapid recognition of previously defined haplotypes, is readily adjustable to additional, newly found restriction patterns and could prove useful in practical breeding programmes.
Landesman
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Department of Animal Sciences, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Uni
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Heller
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ED
D000818:Animals; D001345:Autoradiography / Q000662:veterinary; D015139:Blotting, Southern / Q000662:veterinary; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005260:Female; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000235:genetics; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008297:Male; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D006649:Histocompatibility Antigens; D004247:DNA