title: Rapid changes in the expression of glial cell proteins caused by experimental retinal detachment.
abstract: We examined the expression of several proteins normally present in Müller's glia after the production of experimental retinal detachment in adult cats. Retinas were detached for one-half to seven days, after which the tissue was processed for correlative immunocytochemistry and biochemistry. Previous studies demonstrated that the intermediate filament proteins glial fibrillary acidic protein and vimentin, increase after long-term retinal detachment (30 to 60 days), whereas glutamine synthetase, carbonic anhydrase C, and cellular retinaldehyde-binding protein all decrease to barely detectable levels. Alterations in Müller cell protein expression are rapid and specific events that can be detected as early as two days after retinal detachment. By seven days, levels of protein expression are similar to those in the long-term retinal detachments. Within the first week after injury the Müller cell processes hypertrophy and begin forming glial scars, which indicates that early intervention may be required to halt or reverse the effects of detachment.
authors.affiliation: Neuroscience Research Institute, University of California, Santa Barbara 93106.
authors.forename: G P
authors.initials: GP
authors.lastname: Lewis
authors.forename: C J
authors.initials: CJ
authors.lastname: Guérin
authors.forename: D H
authors.initials: DH
authors.lastname: Anderson
authors.forename: B
authors.initials: B
authors.lastname: Matsumoto
authors.forename: S K
authors.initials: SK
authors.lastname: Fisher
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002256:Carbonic Anhydrases / Q000378:metabolism; D002415:Cats; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism* / Q000473:pathology; D012160:Retina / Q000378:metabolism / Q000473:pathology; D012163:Retinal Detachment / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D012177:Retinol-Binding Proteins / Q000378:metabolism
chemical_list: D007381:Intermediate Filament Proteins; D012177:Retinol-Binding Proteins; D002256:Carbonic Anhydrases; D005974:Glutamate-Ammonia Ligase