title: Genetic analysis of HIV-1 isolates from Brazil reveals presence of two distinct genetic subtypes.
abstract: The spread of the human immunodeficiency virus type 1 (HIV-1) is by now virtually worldwide. An understanding of the genetic, biological, and immunological differences among isolates collected in different geographic locales is crucial for the development of globally effective vaccines. Here we report the genetic characteristics of 21 HIV-1 isolates from Brazil. The isolates were initially characterized using a heteroduplex mobility assay. The majority (17 of 21) were related to North American/European reference isolates of genetic subtype B. Four isolates belonged to a more recently identified genotype, termed subtype F. The subtype F sequences from Brazil are distinguishable in both gag and env from five other genetic subtypes of HIV-1 currently recognized. Like many locales, Brazil harbors more than one HIV-1 subtype.
authors.affiliation: Henry M. Jackson Foundation Research Laboratory, Rockville, Maryland 20850.
authors.forename: J
authors.initials: J
authors.lastname: Louwagie
authors.forename: E L
authors.initials: EL
authors.lastname: Delwart
authors.forename: J I
authors.initials: JI
authors.lastname: Mullins
authors.forename: F E
authors.initials: FE
authors.lastname: McCutchan
authors.forename: G
authors.initials: G
authors.lastname: Eddy
authors.forename: D S
authors.initials: DS
authors.lastname: Burke
mesh_terms: D016915:AIDS Vaccines / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001938:Brazil; D015686:Gene Products, env / Q000235:genetics; D015683:Gene Products, gag / Q000235:genetics; D015752:Genes, env; D015750:Genes, gag; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015658:HIV Infections / Q000382:microbiology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D010446:Peptide Fragments / Q000235:genetics; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D016915:AIDS Vaccines; D015686:Gene Products, env; D015683:Gene Products, gag; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D009692:Nucleic Acid Heteroduplexes; D010446:Peptide Fragments