Application of high field localised in vivo 1H MRS to study biochemical changes in the thiamin deficient rat brain under glucose load.
In vivo, volume-selected 1H NMR spectroscopy employing the SPACE technique was used to monitor biochemical changes in the thiamin deficient rat brain in response to glucose loading. The concentrations of brain N-acetylaspartate, glutamate/glutamine/gamma-aminobutyric acid, lactate and glucose differed significantly from those of control animals. The results are consistent with a metabolic block at the reaction catalyzed by the thiamin dependent enzyme alpha-keto glutarate dehydrogenase soon after the onset of neurological symptoms of thiamin deficiency, and a further block at pyruvate dehydrogenase arising late in the course of thiamin deficiency.
Rose
S E
SE
Centre for Magnetic Resonance, University of Queensland, St. Lucia, Australia.
Nixon
P F
PF
Zelaya
F O
FO
Wholohan
B T
BT
Zimitat
C
C
Moxon
L N
LN
Crozier
S
S
Brereton
I M
IM
Doddrell
D M
DM
D000140:Acidosis, Lactic / Q000209:etiology; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000032:analysis; D001259:Ataxia / Q000209:etiology; D001923:Brain Chemistry; D005260:Female; D005947:Glucose / Q000032:analysis / Q000494:pharmacology* / Q000633:toxicity; D005971:Glutamates / Q000032:analysis; D018698:Glutamic Acid; D005973:Glutamine / Q000032:analysis; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D007773:Lactates / Q000032:analysis; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D011745:Pyrithiamine / Q000633:toxicity; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013832:Thiamine Deficiency / Q000150:complications / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D005971:Glutamates; D007773:Lactates; D011768:Pyruvate Dehydrogenase Complex; D005973:Glutamine; D001224:Aspartic Acid; D019344:Lactic Acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D011745:Pyrithiamine; C000179:N-acetylaspartate; D007655:Ketoglutarate Dehydrogenase Complex; D005947:Glucose