title: Wheat acetyl-coenzyme A carboxylase: cDNA and protein structure.
abstract: cDNA fragments encoding part of wheat (Triticum aestivum) acetyl-CoA carboxylase (ACC; EC 6.4.1.2) were cloned by PCR using primers based on the alignment of several biotin-dependent carboxylases. A set of overlapping clones encoding the entire wheat ACC was then isolated by using these fragments as probes. The cDNA sequence contains a 2257-amino acid reading frame encoding a 251-kDa polypeptide. The amino acid sequence of the most highly conserved domain, corresponding to the biotin carboxylases of prokaryotes, is 52-55% identical to ACC of yeast, rat, and diatom. Identity with the available C-terminal amino acid sequence of maize ACC is 66%. The biotin attachment site has the typical eukaryotic EVMKM sequence. The cDNA does not encode an obvious chloroplast targeting sequence. Various cDNA fragments hybridize in Northern blots to a 7.9-kb mRNA. Southern analysis with cDNA probes revealed multiple hybridizing fragments in hexaploid wheat DNA. Some of the wheat cDNA probes also hybridize with ACC-specific DNA from other plants, indicating significant conservation among plant ACCs.
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
authors.forename: P
authors.initials: P
authors.lastname: Gornicki
authors.forename: J
authors.initials: J
authors.lastname: Podkowinski
authors.forename: L A
authors.initials: LA
authors.lastname: Scappino
authors.forename: J
authors.initials: J
authors.lastname: DiMaio
authors.forename: E
authors.initials: E
authors.lastname: Ward
authors.forename: R
authors.initials: R
authors.lastname: Haselkorn
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
chemical_list: D004247:DNA; D000103:Acetyl-CoA Carboxylase