Effect of fatty acid binding proteins on developing human placental malate dehydrogenase activity.
Role of fatty acid binding proteins (FABPs) in modulating inhibition of human placental malate dehydrogenase by palmitoyl-CoA and oleate has been studied. Activity of human placental cytosolic malate dehydrogenase is detected throughout the gestation, showing a peak at midgestation (20-25 weeks). Inhibition (50%) of the enzyme activity is obtained by 20 microM palmitoyl-CoA or 35 microM oleate. FABPs enhance the activity of malate dehydrogenase in absence of palmitoyl-CoA or oleate and also protect against palmitoyl-CoA or oleate inhibition. Such a modulatory effect of FABP may be due to the binding of long chain fatty acyl-CoA or fatty acid rather than a direct effect of FABPs on the enzyme.
Bandyopadhyay
A K
AK
Department of Biochemistry, University College of Science, Calcutta, India.
Das
T
T
Sa
G
G
Mukherjea
M
M
D002352:Carrier Proteins / Q000494:pharmacology*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000494:pharmacology*; D005260:Female; D005865:Gestational Age; D006801:Humans; D008291:Malate Dehydrogenase / Q000378:metabolism*; D009363:Neoplasm Proteins; D010920:Placenta / Q000201:enzymology*; D010929:Placentation; D011247:Pregnancy; D025521:Tumor Suppressor Proteins
D002352:Carrier Proteins; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins; D008291:Malate Dehydrogenase