The role of protein kinase C in inducing Alzheimer's A68 protein expression in the culture of human neuroblastoma cells.
By using the monoclonal antibody Alz50, we studied the role of protein kinase C (PKC) in inducing A68 protein expression in a human neuronal cell line. Both phorbol 12-myristate 13-acetate and H7 strongly induced A68 protein, while HA1004 and calphoslin C had no effect. The results suggest that the inhibitor and activator of PKC induce A68 protein expression by different mechanisms.
Zhang
L
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Lab. of Clinical Science, NIMH/NIH, Baltimore, MD 21224.
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000911:Antibodies, Monoclonal; D006801:Humans; D015151:Immunoblotting; D007120:Immunochemistry; D007546:Isoquinolines / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D013449:Sulfonamides; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D016875:tau Proteins
D000911:Antibodies, Monoclonal; D007546:Isoquinolines; C054369:MAPT protein, human; D009419:Nerve Tissue Proteins; D010879:Piperazines; D013449:Sulfonamides; D016875:tau Proteins; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate