[Significance of DNA endonucleases in the pathogenesis of ischemic lesion of the brain during massive hemorrhage].
Experiments on dogs exposed to hemorrhagic shock demonstrated a direct relationship between the duration of arterial hypertension and activities of Ca(2+)-Mg(2+)-dependent DNA-endonucleases in the cellular nuclei of the cerebral cortex. Appreciable differences in the kinetics of accumulation of acid-soluble products of DNA chromatin in the cellular nuclei of the cerebral cortex were detected: chromatin DNA degradation was the most rapid in the nervous tissue of dogs exposed to 6 h hemorrhagic shock. Verapamil, a Ca blocker, injected to animals 30 min before massive hemorrhage in a dose of 0.1 mg/kg, reliably reduced the activities of Ca(2+)--Mg(2+)-dependent endonucleases of the cellular nuclei of the cerebral cortex by the 4th h of hemorrhagic shock. The detected physicochemical changes in the chromatin DNA of the cerebral cortex cellular nuclei and a progressive increase of activities of Ca(2+)--Mg(2+)-dependent endonucleases during a prolonged hemorrhagic shock are by their nature close to the phenomenon of genomic programmed cellular death.
Kozhura
V L
VL
Nosova
N V
NV
Novoderzhkina
I S
IS
Solov'eva
Zh V
ZhV
D000818:Animals; D016923:Cell Death / Q000235:genetics; D002467:Cell Nucleus / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology*; D002843:Chromatin / Q000201:enzymology / Q000235:genetics; D004247:DNA / Q000235:genetics; D004285:Dogs; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D005260:Female; D008297:Male; D012771:Shock, Hemorrhagic / Q000209:etiology / Q000378:metabolism*; D013997:Time Factors
D002843:Chromatin; D004247:DNA; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
Znachenie DNK-éndonukleoliza v patogeneze ishemicheskogo porazheniia mozga pri massivnoĭ krovopotere.