Study on replicase (subunit) gene of papaya ringspot virus cloning, sequencing and construction of higher plant expression vector.
The ds-cDNA was synthesized using genomic RNA of papaya ringspot virus (PRSV) as template. The blunt-ended cDNA was cloned into the EcoRV site of vector pBluescript SK. From the recombinants, the NIb gene of PRSV was obtained, and its complete sequence was also determined. After the NIb gene was modified through PCR, a binary vector of PRSV-NIb gene was constructed for its expression in higher plants.
Liu
J
J
Laboratory of Plant Biotechnology, Chinese Academy of Sciences, Beijing.
Peng
X
X
Mang
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017800:Potyvirus / Q000235:genetics*; D018749:RNA, Plant / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
D018076:DNA, Complementary; D018749:RNA, Plant; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase; C061396:nuclear inclusion protein b, mosaic viruses; D012321:DNA-Directed RNA Polymerases