The concentration of LH-RH receptors in the nuclei of pancreatic cancer cells. Effect of (D-Trp6)LH-RH on tumor-bearing Syrian golden hamsters.
LH-RH analogs cause some inhibition of growth of pancreatic cancers. Syrian golden hamsters bearing chemically induced pancreatic cancers were treated with [D-Trp6]LH-RH for 3 d before sacrifice. LH-RH receptors were localized by electron-microscopic immunohistochemistry in the tumor cells of both treated and untreated hamsters. [D-Trp6]LH-RH treatment resulted in a marked increase in the concentration of LH-RH receptors in the nuclei. The dissociation constants (Kd) and the maximal binding capacity of the LH-RH receptors (Bmax), measured by radioreceptor assay, were higher in the nuclei of the pancreatic tumor cells of hamsters treated with [D-Trp6]LH-RH than in the untreated controls. Pancreatic cells of tumor-free hamsters did not show immunostaining for LH-RH receptors. A possible correlation between the increase in the concentration of the LH-RH receptors in the nuclei and the tumor growth-inhibiting activity of [D-Trp6]LH-RH is suggested.
Szende
B
B
Institute of Pathology and Experimental Cancer Research, Semmelweis University of Medicine, Budapest, Hungary.
Csikós
A
A
Szepeshazi
K
K
Neill
J D
JD
Mulchahey
J J
JJ
Halmos
G
G
Lapis
K
K
Schally
A V
AV
D000818:Animals; D002273:Carcinogens; D002467:Cell Nucleus / Q000378:metabolism; D006224:Cricetinae; D005260:Female; D007150:Immunohistochemistry; D008647:Mesocricetus; D016253:Microscopy, Immunoelectron; D009602:Nitrosamines; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000737:chemistry* / Q000648:ultrastructure; D011869:Radioligand Assay; D011966:Receptors, LHRH / Q000378:metabolism*; D017329:Triptorelin Pamoate / Q000494:pharmacology
D002273:Carcinogens; D009602:Nitrosamines; D011966:Receptors, LHRH; D017329:Triptorelin Pamoate; C013622:nitrosobis(2-oxopropyl)amine