A purine permease in Candida glabrata.
Competition experiments revealed that adenine and guanine were transported by a purine permease in both Candida glabrata 4 and a C. glabrata 4 cytosine permease negative mutant. The C. glabrata 4 cytosine permease negative mutant was isolated using 5-fluorocytosine selection. This mutant no longer transported cytosine, but transported adenine and guanine. A transport system for hypoxanthine was not detected. Hence, in addition to the cytosine permease, a purine permease exists in C. glabrata. This differs from the purine cytosine permeases in Saccharomyces cerevisiae and Candida albicans which transport adenine, cytosine, guanine and hypoxanthine.
Sen Gupta
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Yale Universtiy School of Medicine, Department of Genetics, New Haven, Connecticut 06510-8005.
Kerridge
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Chevallier
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MR
D000225:Adenine / Q000378:metabolism; D002175:Candida / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D003596:Cytosine / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D009154:Mutation; D033702:Nucleobase Transport Proteins; D011687:Purines / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013045:Species Specificity
D026901:Membrane Transport Proteins; D033702:Nucleobase Transport Proteins; D011687:Purines; C022483:purine permease; D003596:Cytosine; D000225:Adenine