title: Adrenalectomy reduces atrial natriuretic peptide stimulated guanylate cyclase activity in rat paraventricular nucleus.
abstract: Atrial natriuretic peptide (ANP) or an Atriopeptin III analog (PL 058) stimulated cGMP formation in the membrane fraction of rat olfactory bulb, median eminence and paraventricular nucleus, in a dose-dependent manner. The effect of the Atriopeptin III analog was 20-40% greater than that of ANP. Bilateral adrenalectomy, with or without mineralo- or glucocorticoid-replacement, on ANP-stimulated cGMP formation was investigated in rat paraventricular nucleus. 11 days after bilateral adrenalectomy a reduced responsiveness to ANP- or PL 058-induced cGMP production was observed. This effect was prevented by deoxycorticosterone, but not by dexamethasone administration. Our results further support the presence of guanylate cyclase-coupled ANP receptors in brain localized target sites; and they provide evidence suggesting that guanylate cyclase-linked ANP binding sites in the PVN are susceptible to regulatory changes after adrenalectomy-induced activation of the hypothalamus-hypophyso-adrenocortical system.
authors.affiliation: Faculty of Pharmacy, Department of Biological Science, Universidad Central de Venezuela, Caracas.
authors.forename: A
authors.initials: A
authors.lastname: Israel
authors.forename: Y
authors.initials: Y
authors.lastname: Mathison
authors.forename: M
authors.initials: M
authors.lastname: Cierco
authors.forename: M R
authors.initials: MR
authors.lastname: Garrido
authors.forename: Y
authors.initials: Y
authors.lastname: Barbella
mesh_terms: D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D006162:Guanylate Cyclase / Q000096:biosynthesis*; D008297:Male; D008473:Median Eminence / Q000201:enzymology; D009830:Olfactory Bulb / Q000201:enzymology; D010286:Paraventricular Hypothalamic Nucleus / Q000201:enzymology*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C051535:PL 058; D010446:Peptide Fragments; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase