Assay of estrogen and progesterone receptors in small samples of breast cancer: I. Mathematical analysis of total binding data.
The quantitative determination of Estrogen and Progesterone receptors in breast cancer is performed using about 500 mg of tissue. Such an amount of tissue sample is available with ever increasing difficulty. In the present paper a mathematical method of analysis of the total (specific+nonspecific) binding data is given. In this way about 50% of tissue can be saved as only one set of test tubes is required; in fact, the method does not need the second set of tubes used to determine nonspecific binding in the conventional assay. Additional advantages from the statistical point of view are also presented.
Piantanelli
L
L
Gerontological Research Department, INRCA, Ancona.
Rossolini
G
G
Viticchi
C
C
Basso
A
A
D000818:Animals; D001943:Breast Neoplasms / Q000737:chemistry*; D002417:Cattle; D005260:Female; D006801:Humans; D008297:Male; D008956:Models, Chemical; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D014599:Uterus / Q000737:chemistry
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone