title: Spatial and temporal changes in signal transduction pathways during LTP.
abstract: Following LTP induction in freely moving rats, in situ hybridization revealed discrete changes in the expression of one isoform in each of four families of serine/threonine kinases constitutively expressed in the dentate gyrus of the hippocampus. Expression of the alpha isoform of CaMKII showed a transient increase over the soma and a more persistent increase over the dendritic field of dentate granule cells. Of the PKC isoforms, only gamma PKC was up-regulated substantially 2 hr after LTP induction, declining to control levels 48 hr later. An increase in the expression of mRNA for ERK2 and raf-B was seen at 24 hr only. These results show that, during the maintenance phase of LTP in the hippocampus, there are selective increases in the expression of serine/threonine kinases and that these increases have specific and characteristic temporal and spatial profiles.
authors.affiliation: Neurobiology Division, Medical Research Council Centre, Cambridge, England.
authors.forename: K L
authors.initials: KL
authors.lastname: Thomas
authors.forename: S
authors.initials: S
authors.lastname: Laroche
authors.forename: M L
authors.initials: ML
authors.lastname: Errington
authors.forename: T V
authors.initials: TV
authors.lastname: Bliss
authors.forename: S P
authors.initials: SP
authors.lastname: Hunt
mesh_terms: D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D003712:Dendrites / Q000201:enzymology; D005786:Gene Expression Regulation; D017781:Genes, Immediate-Early; D006098:Granulocytes / Q000201:enzymology; D006624:Hippocampus / Q000201:enzymology / Q000502:physiology; D007527:Isoenzymes / Q000378:metabolism; D017774:Long-Term Potentiation; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases