title: Isolation of a new H3.3 histone variant cDNA of P. lividus sea urchin: sequence and embryonic expression.
abstract: A cDNA encoding a new H3 histone variant has been isolated from a Paracentrotus lividus sea urchin embryo cDNA library. The encoded protein is identical to the H3.3 histone subtype identified in other species, with the difference that E replaces D at position 81. The clone corresponds to a transcript of about 1.6 kb, not dependent on DNA replication, present in the unfertilized egg and at all stages of embryonic development. The coding part of the cDNA cross-reacts also with a 0.5 kb H3 late histone mRNA.
authors.affiliation: Department of Genetics, General and Molecular Biology, University of Naples Federico II, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Fucci
authors.forename: F
authors.initials: F
authors.lastname: Aniello
authors.forename: M
authors.initials: M
authors.lastname: Branno
authors.forename: E
authors.initials: E
authors.lastname: Biffali
authors.forename: G
authors.initials: G
authors.lastname: Geraci
mesh_terms: D000818:Animals; D001483:Base Sequence; D002970:Cleavage Stage, Ovum; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012617:Sea Urchins / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D006657:Histones; D012333:RNA, Messenger