Biochemical characteristics of primary and passaged cultures of primate brain microvessel endothelial cells.
Rhesus macaque monkey brain microvessel endothelial cells (BMECs) were isolated and grown in culture in an effort to establish an appropriate primate in vitro model of the endothelial component of the blood-brain barrier. The presence of Factor VIII antigen, alkaline phosphatase, gamma-glutamyl transpeptidase, lactate dehydrogenase, total protein, and the passive permeability properties was documented for both primary and passaged cultures. Primate BMECs were shown to exhibit similar morphological and biochemical properties described for other BMEC culture systems derived from other species. In addition, the passaged primate BMECs were particularly notable for the changes in enzyme activities and total protein that parallel age-dependent changes in brain capillary endothelia. This study provides further support for the possible application of BMEC culture systems in investigations of blood-brain barrier functions under normal, aging, and diseased conditions.
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Department of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas Lawrence 66045.
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D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000098:blood supply*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005169:Factor VIII / Q000378:metabolism; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008253:Macaca mulatta; D008833:Microcirculation; D009419:Nerve Tissue Proteins / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D013045:Species Specificity; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D009419:Nerve Tissue Proteins; D005169:Factor VIII; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D007931:Leucyl Aminopeptidase; D007703:Peptidyl-Dipeptidase A