Modulation of EGF receptor and CD15 (Lewisx) antigen on the cell surface of breast carcinoma cell lines induced by cytokines, retinoic acid, 12-O-tetradecanoylphorbol 13-acetate and 1,25(OH)2-vitamin D3.
Interleukin (IL) 1 alpha induced the up-regulation of cell-surfac-expressed epidermal growth factor (EGF) receptor on both MDA-MB-468 and BT-20 breast cancer cell lines. IL-1 beta and tumor necrosis factor alpha (TNF-alpha) increased the EGF receptor surface expression only on BT-20 breast carcinoma cells. 12-O-tetradecanoylphorbol 13-acetate (TPA) induced up-regulation of EGF receptor on MDA-MB-468 cells, and a marginal but significant increase was determined in BT-20 cells and in interferon gamma (IFN-gamma)-treated MDA-MB-468 cells. CD15 (Lewisx) antigen was down-regulated on MDA-MB-468 cells by TNF-alpha, TPA, IL-1 alpha, as well as by IFN-gamma and all-trans-retinoic acid. 1,25(OH)2-vitamin D3 up-regulated the CD15 antigen surface expression on MDA-MB-468 cells.
Sedlak
J
J
Cancer Research Institute, Bratislava, Slovakia.
Speiser
P
P
Hunakova
L
L
Duraj
J
J
Chorvath
B
B
D015703:Antigens, CD / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000276:immunology / Q000378:metabolism*; D002117:Calcitriol / Q000494:pharmacology*; D016207:Cytokines / Q000494:pharmacology*; D015536:Down-Regulation; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D016256:Lewis X Antigen; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015854:Up-Regulation
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D014408:Biomarkers, Tumor; D016207:Cytokines; D007375:Interleukin-1; D016256:Lewis X Antigen; D014409:Tumor Necrosis Factor-alpha; D014212:Tretinoin; D007371:Interferon-gamma; D066246:ErbB Receptors; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate