Flow injection analysis for glucose using an amperometric enzyme electrode based on lipid-modified glucose oxidase as the detector.
The concentration of glucose is determined by a combination of flow-injection analysis with amperometric enzyme sensor detection. The enzyme sensor is prepared by coating a glassy carbon electrode with a layer of lipid-modified glucose oxidase and Nafion: first, a benzene solution of the modified enzyme is placed on the glassy carbon electrode and dried, then a Nafion solution is placed on the electrode and dried. The sensor-based system exhibited a linear response for glucose concentration up to 10 mM with a sampling rate of 250 sample/h, and is stable for 12 weeks after 2000 glucose injections.
Mizutani
F
F
National Institute for Bioscience and Human-Technology, Ibaraki, Japan.
Yabuki
S
S
D001628:Beverages / Q000032:analysis; D015374:Biosensing Techniques; D004563:Electrochemistry; D017022:Flow Injection Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D008055:Lipids
D008055:Lipids; D005949:Glucose Oxidase; D005947:Glucose