title: Use of firefly luciferase gene for plasmid copy number determination.
abstract: A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimate plasmid copy number by densitometry is also described.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Coronado
authors.forename: M E
authors.initials: ME
authors.lastname: Vázquez
authors.forename: A
authors.initials: A
authors.lastname: Cebolla
authors.forename: A J
authors.initials: AJ
authors.lastname: Palomares
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000201:enzymology / Q000235:genetics*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005784:Gene Amplification; D015870:Gene Expression; D017344:Genes, Insect; D017930:Genes, Reporter; D005819:Genetic Markers; D005821:Genetic Techniques; D005822:Genetic Vectors; D008156:Luciferases / Q000235:genetics*; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D005819:Genetic Markers; D008156:Luciferases