Chromosome preparations of both direct and long-term cultures from the same fragment of chorionic villi.
A simplified method is described for processing both direct preparations and long-term cultures from the same fragment of chorionic villi. Enzyme separation of the outer trophoblast layers (used for direct preparations) from the inner mesenchymal core (used to initiate long-term cultures) facilitates the utilization of the same fragments for the two procedures, without jeopardizing the success of either method. This has proved useful in cases where the sample was so small that only one method of chromosome preparation may have been possible using other techniques.
Dawson
J M
JM
Regional Cytogenetics Unit, Birmingham Maternity Hospital, Edgbaston, U.K.
Griffiths
M J
MJ
D002478:Cells, Cultured; D002824:Chorionic Villi / Q000648:ultrastructure*; D015193:Chorionic Villi Sampling; D002869:Chromosome Aberrations; D002875:Chromosomes; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D011247:Pregnancy; D011296:Prenatal Diagnosis; D014327:Trophoblasts / Q000648:ultrastructure