A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification.
A polymorphism was detected in the 3' untranslated region of the bovine gamma-S-crystallin gene by direct sequencing of polymerase chain reaction (PCR) products from genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give allele-specific amplification. The two allele-specific primers differ in length by 20 nucleotides so that the allelic products may be distinguished by simple agarose gel electrophoresis following a single PCR reaction. This provides a simple and rapid assay for this polymorphism.
Kemp
S J
SJ
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Maillard
J C
JC
Teale
A J
AJ
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003459:Crystallins / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D008297:Male; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic
D003459:Crystallins; D004277:DNA, Single-Stranded; D005819:Genetic Markers; D009841:Oligonucleotides