Cloning and expression of a gene encoding gelonin, a ribosome-inactivating protein from Gelonium multiflorum.
A cDNA copy of the gel gene, encoding gelonin (Gel), has been cloned from the seeds of the Asian plant, Gelonium multiflorum. Gel is a type-I ribosome-inactivating protein which has been produced in Escherichia coli as a secreted protein under the transcriptional control of the Salmonella typhimurium araB promoter and linked to the pectate lyase (pelB) leader sequence from Erwinia carotovora. Recombinant, soluble Gel (re-Gel) can be recovered from the E. coli culture supernatant at a yield of greater than 1 mg/ml, and it inhibits protein synthesis in vitro to the same extent as the native protein isolated from plant seeds.
Nolan
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XOMA Corporation, Santa Monica, CA 90404.
Garrison
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Better
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004926:Escherichia coli; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D011133:Polysaccharide-Lyases / Q000235:genetics; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D054789:Ribosome Inactivating Proteins, Type 1; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D010940:Plant Proteins; D011994:Recombinant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum; D004247:DNA; D011133:Polysaccharide-Lyases; C023911:pectate lyase