Determination of a new antibronchospastic agent, MX2/120, in guinea pig plasma by high-performance liquid chromatography in a pharmacokinetic study.
7-[(2,2-Dimethyl)propyl)]-1-methylxanthine (I, Lab code MX2/120) is a new potent antibronchospastic agent. A rapid and simple HPLC assay for I in guinea pig plasma has been developed. Compound I was extracted from plasma with dichloromethane by a solid-phase extraction procedure, after adding 1,3-dimethyl-7-pentylxanthine at a concentration of 5 micrograms/ml as the internal standard (I.S.). The extraction residue was redissolved in water-acetonitrile and chromatographed on a RP-18 reversed-phase column. The eluate was monitored by spectrophotometric detection at 280 nm. The method showed good linearity over the range 0.1-20 micrograms/ml (r = 0.9998) and is precise (C.V. x Student's t-test = 1.84%) and accurate (mean recovery +/- limit of confidence = 100.25 +/- 0.34). The HPLC assay was successfully applied to the determination of the pharmacokinetic profile of I after intravenous and oral administration in guinea pigs. The main pharmacokinetic parameters are presented.
Agostini
O
O
Research Laboratories, Analytical Department Istituto Farmacobiologico Malesci S.p.A., Florence, Italy.
Toja
E
E
D000284:Administration, Oral; D000818:Animals; D001993:Bronchodilator Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006168:Guinea Pigs; D007262:Infusions, Intravenous; D008297:Male; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014970:Xanthines / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
C092650:7-((2,2-dimethyl)propyl)-1-methylxanthine; D001993:Bronchodilator Agents; D014970:Xanthines