Radiation-induced genomic instability: delayed cytogenetic aberrations and apoptosis in primary human bone marrow cells.
Transmissible chromosomal instability, characterized by non-clonal cytogenetic aberrations with a high frequency of chromatid-type aberrations together with a lower frequency of chromosome-type aberrations, has been demonstrated in the clonal descendants of human haemopoietic stem cells after alpha- but not X-irradiation. Comparable cytogenetic abnormalities have also been demonstrated in non-clonal cultures of alpha-irradiated primary human bone marrow, but a different pattern of delayed aberrations, mainly of chromosome-type, was found after X-irradiation in non-clonal cultures. In clonal analyses, delayed apoptotic cell death was evident after both X- and alpha-irradiation. It is suggested that the type of radiation exposure, the type of cell and its genetically determined susceptibility are factors that may influence the expression of delayed effects of radiation.
Kadhim
M A
MA
MRC Radiobiology Unit, Chilton, Didcot, UK.
Lorimore
S A
SA
Townsend
K M
KM
Goodhead
D T
DT
Buckle
V J
VJ
Wright
E G
EG
D000512:Alpha Particles; D017209:Apoptosis / Q000528:radiation effects*; D001853:Bone Marrow / Q000528:radiation effects*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002842:Chromatids / Q000528:radiation effects; D002869:Chromosome Aberrations; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000528:radiation effects / Q000648:ultrastructure; D006801:Humans; D014965:X-Rays