title: Serum enzyme activities in full-term asphyxiated and healthy newborns: enzyme kinetics during the first 144 hours of life.
abstract: Little is known about the kinetics of most serum enzymes during the first hours of life, and even less about the effect on such enzyme activities of perinatal hypoxia-ischaemia. It was the aim of the present study to evaluate the serum kinetics of seven differently located cell enzymes in healthy and asphyxiated newborns during the 1st week of life. The serum activities of cytoplasmic and mitochondrial [aspartate aminotransferase (ASAT), creatine kinase (CK), glutamate dehydrogenase (GLDH), lactate dehydrogenase (LDH), and hydroxybutyrate dehydrogenase (HBDH)] and membrane-bound (gamma-glutamyl-transferase and leucine arylaminidase) enzymes were prospectively measured in full-term asphyxiated (n = 49) and healthy (n = 87) newborns during the first 144 h of life. The blood samples were taken serially at five fixed times: 0 (cord), 12, 24, 72, and 144 h postpartum. The asphyxiated newborns had significantly increased serum activities of ASAT, LDH, and HBDH up to 72 h postpartum, whereas healthy newborns showed higher CK and GLDH activities. Only the activities of ASAT, LDH, and HBDH seemed to depend on the oxygen supply of the fetus or newborn. If other causes of increased serum enzyme activities, e.g. liver diseases, haemolytic disorders, tumours, or inborn errors of metabolism, are excluded, elevated serum activities of ASAT, LDH, and HBDH should draw one's attention to a perinatal hypoxic-ischaemic insult of the newborn.
authors.affiliation: Department of Paediatrics, Städtisches Klinikum Fulda, Teaching Hospital of the University of Marburg, FRG.
authors.forename: G M
authors.initials: GM
authors.lastname: Lackmann
authors.forename: U
authors.initials: U
authors.lastname: Töllner
authors.forename: R
authors.initials: R
authors.lastname: Mader
mesh_terms: D000375:Aging / Q000378:metabolism*; D001034:Apgar Score; D001219:Aspartate Aminotransferases / Q000097:blood; D001238:Asphyxia Neonatorum / Q000097:blood / Q000201:enzymology*; D004796:Clinical Enzyme Tests; D003402:Creatine Kinase / Q000097:blood; D004798:Enzymes / Q000097:blood*; D005969:Glutamate Dehydrogenase / Q000097:blood; D006801:Humans; D006884:Hydroxybutyrate Dehydrogenase / Q000097:blood; D007231:Infant, Newborn / Q000378:metabolism*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007931:Leucyl Aminopeptidase / Q000097:blood; D012016:Reference Values; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D004798:Enzymes; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D005969:Glutamate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase; D007931:Leucyl Aminopeptidase