Direct stereochemical resolution of SM-11044, a novel anti-asthmatic drug, and its stereoisomers using a chiral immobilized protein stationary phase.
A high-performance liquid chromatographic separation of SM-11044, a novel anti-asthmatic drug, and its antipode and stereoisomers was achieved using a chiral protein column that permits low levels of the antipode to be measured in the SM-11044. The influence of replacing H2O with 2H2O as the mobile phase and the effect of buffer ionic strength, pH, organic modifiers and temperature on the retention times and enantiomeric resolution are discussed.
Okamoto
M
M
Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., Osaka, Japan.
Ohgami
Y
Y
Nakazawa
H
H
D000318:Adrenergic beta-Agonists / Q000032:analysis*; D001993:Bronchodilator Agents / Q000032:analysis*; D002396:Catechols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D012694:Serine / Q000031:analogs & derivatives* / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013696:Temperature
D000318:Adrenergic beta-Agonists; D001993:Bronchodilator Agents; D002396:Catechols; C076952:SM 11044; D012694:Serine