Pharmacologic characterization of [3H]dopamine and [3H]spiperone binding in mouse cerebellum.
1. [3H]Dopamine and [3H]spiperone binding to cerebellar homogenates was characterized utilizing dopaminergic agonists, antagonists and non-dopaminergic drugs. 2. The [3H]DA binding to low affinity binding sites reveals a heterogeneous population consisting of dopaminergic as well as serotonergic and noradrenergic sites. However, the high affinity binding of [3H]DA reflects dopaminergic sites, although a small contribution of serotonergic and noradrenergic binding sites cannot be excluded. 3. [3H]Spiperone also labels a heterogeneous population of binding sites which, however, are mainly dopaminergic.
Panagopoulos
N T
NT
Department of Biology, University of Patras, Greece.
Matsokis
N A
NA
D000818:Animals; D001667:Binding, Competitive; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism* / Q000494:pharmacology; D015259:Dopamine Agents / Q000378:metabolism / Q000494:pharmacology; D018492:Dopamine Antagonists; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D013134:Spiperone / Q000378:metabolism* / Q000494:pharmacology; D014316:Tritium
D015259:Dopamine Agents; D018492:Dopamine Antagonists; D014316:Tritium; D013134:Spiperone; D004298:Dopamine