title: Characterization of a cryptic extrachromosomal element isolated from the mollicute Spiroplasma taiwanense.
abstract: Characterization of an extrachromosomal element from an organism in the genus Spiroplasma is likely to be an essential step in the development of cloning vectors which replicate in these organisms. A restriction map for an 11-kb element, designated pCT-1, isolated from Spiroplasma taiwanese strain CT-1 (ATCC 43302) has been constructed using the restriction enzymes Bg/II, EcoRI, HincII, HindIII, HpaI, PstI, and XbaI. This element is distinct from any previously characterized spiroplasma virus or plasmid. pCT-1 has been cloned into the Escherichia coli vector pBR322 as a step in the development of a biphasic shuttle vector system.
authors.affiliation: Insect Biocontrol Laboratory, USDA, Beltsville, Maryland 20705.
authors.forename: G E
authors.initials: GE
authors.lastname: Gasparich
authors.forename: K J
authors.initials: KJ
authors.lastname: Hackett
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003465:Culex / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005111:Extrachromosomal Inheritance; D005822:Genetic Vectors; D015183:Restriction Mapping; D013149:Spiroplasma / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial