title: Dynamic properties of nuclear lamins: lamin B is associated with sites of DNA replication.
abstract: The nuclear lamins form a fibrous structure, the nuclear lamina, at the periphery of the nucleus. Recent results suggest that lamins are also present as foci or spots in the nucleoplasm at various times during interphase of the cell cycle (Goldman, A. E., R. D. Moir, M. Montag-Lowy, M. Stewart, and R. D. Goldman. 1992. J. Cell Biol. 104:725-732; Bridger, J. M., I. R. Kill, M. O'Farrell, and C. J. Hutchison. 1993. J. Cell Sci. 104:297-306). In this report we demonstrate that during mid-late S-phase, nuclear foci detected with lamin B antibodies are coincident with sites of DNA replication as detected by the colocalization of sites of incorporation of bromodeoxyuridine (BrDU) or proliferating cell nuclear antigen (PCNA). The relationship between lamin B and BrDU is not maintained in the following G1 stage of the cell cycle. Furthermore, the nuclear staining patterns seen with antibodies directed against lamins A and C in mid-late S-phase do not coalign with the lamin B/BrDU-containing structures. These results imply that there is a role for lamin B in the organization of replicating chromatin during S phase.
authors.affiliation: Department of Cell, Molecular, and Structural Biology, Northwestern University Medical School, Chicago, Illinois 60611.
authors.forename: R D
authors.initials: RD
authors.lastname: Moir
authors.forename: M
authors.initials: M
authors.lastname: Montag-Lowy
authors.forename: R D
authors.initials: RD
authors.lastname: Goldman
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D002478:Cells, Cultured; D006224:Cricetinae; D004261:DNA Replication / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D007399:Interphase / Q000502:physiology; D034921:Lamin Type B; D034882:Lamins; D051379:Mice; D009687:Nuclear Proteins / Q000276:immunology / Q000302:isolation & purification*; D018809:Proliferating Cell Nuclear Antigen; D016196:S Phase / Q000502:physiology*
chemical_list: D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D001973:Bromodeoxyuridine