A simple and rapid method for the purification of GroEL, an Escherichia coli homolog of the heat shock protein 60 family of molecular chaperonins.
GroEL, an Escherichia coli homolog of the heat shock protein 60 family of molecular chaperonins, has been implicated as a target of T cell-mediated immune responses in a broad spectrum of infections. In order to produce large quantities of native protein for raising and stimulating GroEL specific T cell lines, we have developed a simple and rapid two-step protocol for purifying native E. coli GroEL heat shock (or stress) protein which takes advantage of the inherent structural and functional properties of the protein. Based on a combination of gel exclusion chromatography, ATPase activity assay, isoelectric focusing, and circular dichroism analyses we conclude that our purification process yields native tetradecameric GroEL.
Khandekar
S S
SS
Procept, Inc., Cambridge, Massachusetts 02139.
Bettencourt
B M
BM
Kelley
K C
KC
Recny
M A
MA
D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000302:isolation & purification*; D018834:Chaperonin 60; D002942:Circular Dichroism; D004926:Escherichia coli / Q000737:chemistry*; D006360:Heat-Shock Proteins / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet
D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D000255:Adenosine Triphosphate