Spatial and temporal changes in signal transduction pathways during LTP.
Following LTP induction in freely moving rats, in situ hybridization revealed discrete changes in the expression of one isoform in each of four families of serine/threonine kinases constitutively expressed in the dentate gyrus of the hippocampus. Expression of the alpha isoform of CaMKII showed a transient increase over the soma and a more persistent increase over the dendritic field of dentate granule cells. Of the PKC isoforms, only gamma PKC was up-regulated substantially 2 hr after LTP induction, declining to control levels 48 hr later. An increase in the expression of mRNA for ERK2 and raf-B was seen at 24 hr only. These results show that, during the maintenance phase of LTP in the hippocampus, there are selective increases in the expression of serine/threonine kinases and that these increases have specific and characteristic temporal and spatial profiles.
Thomas
K L
KL
Neurobiology Division, Medical Research Council Centre, Cambridge, England.
Laroche
S
S
Errington
M L
ML
Bliss
T V
TV
Hunt
S P
SP
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D003712:Dendrites / Q000201:enzymology; D005786:Gene Expression Regulation; D017781:Genes, Immediate-Early; D006098:Granulocytes / Q000201:enzymology; D006624:Hippocampus / Q000201:enzymology / Q000502:physiology; D007527:Isoenzymes / Q000378:metabolism; D017774:Long-Term Potentiation; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013997:Time Factors
D007527:Isoenzymes; D012333:RNA, Messenger; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases