A rapid semiautomatical enzyme linked immunoassay identifying intercellular adhesion molecule-1 (ICAM-1) on the alveolar macrophage surface.
Intercellular adhesion molecule-1 (ICAM-1), a member of the immunoglobulin gene superfamily, is a cytokine-inducible adhesion molecule, which plays a central role in leukocyte migration into sites of acute or chronic inflammation. In this article we describe a sandwich immunoenzymometric method which allows rapid, semiquantitative (in "enzyme immunoassay units", EU) identification of ICAM-1 on the surface of alveolar macrophages. We evaluated this method in two groups of patients with pulmonary sarcoidosis (n = 12) or bacterial pneumonia (n = 11) and a group of healthy volunteers (n = 6), comparing the results with those obtained by immunocytochemical staining. ICAM-1 expression on the sarcoid alveolar macrophages surface was significantly elevated, as compared with control alveolar macrophages (0.76 EU +/- 0.27 vs. 0.44 EU +/- 0.12, p < 0.01). ICAM-1 expression on the surface of alveolar macrophages from patients with pneumonia was not elevated (0.48 EU +/- 0.35). Stimulation with tumour necrosis factor-alpha (TNF-alpha) or interferon-gamma (100 kU/l) led to a significant induction of ICAM-1 on the surface of control alveolar macrophages (0.76 EU +/- 0.18, p < 0.005 for TNF-alpha, 0.64 EU +/- 0.10, p < 0.005 for interferon-gamma), whereas alveolar macrophages from both patient groups did not respond to cytokines even at high dosages. ICAM-1 expression on the surface of alveolar macrophages from patients with sarcoidosis correlated with the spontaneous release of TNF-alpha by macrophages (R = 0.77, p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Kamp
S
S
Klinik für Innere Medizin, Medizinische Universität zu Lübeck, Germany.
Kreft
B
B
Braun
J
J
Dalhoff
K
K
D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002462:Cell Membrane / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D007371:Interferon-gamma / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D018410:Pneumonia, Bacterial / Q000276:immunology*; D017565:Sarcoidosis, Pulmonary / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma