[Tyrosine hydroxylase and dopa decarboxylase expression in the neurons of a mediobasal hypothalamic transplant].
Specific antibodies were used for studying the expression of tyrosine hydroxylase (TH) and DOPA decarboxylase (DDC) in neurons of rat embryonic mediobasal hypothalamus (MBH), whose fragments were implanted into the third brain ventricle of the adult female Wistar rats. Two months after surgery, most immunopositive neurons expressed TH, smaller number of neurons expressed both TH and DDC, and few cells contained only DDC. As during development in situ, immunopositive neurons formed nuclei-like clusters located in the ventral region of the graft. This data suggest that MBH grafts contain a population of dopaminergic neurons and a population of TH-containing neurons that probably are not dopaminergic.
Fetisov
S O
SO
D000818:Animals; D016380:Brain Tissue Transplantation / Q000502:physiology*; D004296:Dopa Decarboxylase / Q000378:metabolism*; D005260:Female; D016332:Fetal Tissue Transplantation / Q000502:physiology*; D007033:Hypothalamus, Middle / Q000196:embryology / Q000201:enzymology* / Q000637:transplantation*; D007150:Immunohistochemistry; D009474:Neurons / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D014446:Tyrosine 3-Monooxygenase; D004296:Dopa Decarboxylase
Ekspressiia tirozingidroksilazy i dopadekarboksilazy v neĭronakh transplanta mediobazal'nogo gipotalamusa.