title: Evaluation of an enzyme immunoassay kit for the detection of Clostridium difficile enterotoxin.
abstract: The Premier Clostridium difficile toxin A enzyme immunoassay (EIA) kit was evaluated for the detection of C. difficile enterotoxin in fecal samples. A total of 314 samples was tested by culture, cytotoxin detection and EIA kit. Compared to a combined culture/cytotoxin result the Premier EIA kit had a sensitivity of 88.3%, a specificity of 100%, a predictive value positive of 100% and a predictive value negative of 87.4%. Test results were available within 3 hrs providing a rapid and reliable means of detecting C. difficile enterotoxin.
authors.affiliation: Department of Microbiology, University of Western Australia, Nedlands.
authors.forename: R A
authors.initials: RA
authors.lastname: Bowman
authors.forename: S
authors.initials: S
authors.lastname: Arrow
authors.forename: L
authors.initials: L
authors.lastname: Croese
authors.forename: T V
authors.initials: TV
authors.lastname: Riley
mesh_terms: D016360:Clostridioides difficile / Q000737:chemistry*; D004768:Enterotoxins / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D004768:Enterotoxins; D011933:Reagent Kits, Diagnostic