Expression of CD11/CD18 and ICAM-1 on monocytes and lymphocytes of HIV-1-infected individuals.
The role of the leukocyte integrins in the infection of cells by HIV-1 or in the progression of AIDS-related disease is of continuing interest. Using a dual-labeling flow cytometric method, we determined the level of expression of the leukocyte integrins and intercellular adhesion molecule 1 (ICAM-1) on monocytes and lymphocytes from HIV-1-infected and uninfected individuals. The mean fluorescence of CD18 on lymphocytes and CD11c on monocytes of HIV-1-infected subjects was significantly higher than for the control group (P = .008 and .014, respectively). There was a trend toward higher fluorescence of CD11a on lymphocytes from HIV-1-infected subjects compared with cells from the control group (P = .089). Integrin expression on lymphocytes or monocytes from HIV-infected individuals did not correlate with their CD4 lymphocyte number. However, the mean fluorescence associated with ICAM-1 on monocytes and CD11a and CD18 expression on lymphocytes was related to clinical stage of disease.
Stent
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G
Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia.
Cameron
P U
PU
Crowe
S M
SM
D000163:Acquired Immunodeficiency Syndrome / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D015703:Antigens, CD / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D018845:CD11 Antigens; D016827:CD8 Antigens / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D005434:Flow Cytometry; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D009000:Monocytes / Q000737:chemistry* / Q000382:microbiology* / Q000473:pathology
D015703:Antigens, CD; D018845:CD11 Antigens; D016827:CD8 Antigens; D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1