Successful bone marrow transplantation by bone grafts in chimeric-resistant combination.
It has been shown that the bone marrow cells (BMCs) of DBA/2 mice do not readily take in bone marrow from C57BL/6 mice, even if more than 5 x 10(6) cells are injected. In this paper, we show that such chimerism resistance can be overcome by cografting DBA/2 bones from which the hematopoietic cells have been removed but in which the stromal cells remain. C57BL/6 mice reconstituted with BMCs plus bones of DBA/2 mice survive significantly longer than C57BL/6 mice reconstituted with DBA/2 BMC alone. In the former, not only the hematolymphoid cells but also the stromal cells are replaced by donor-type cells, whereas in the latter, the donor hematolymphoid cells are rejected. These findings indicate that the stromal cells in the engrafted bones play a crucial role in the prevention of graft rejection in bone marrow transplantation.
Hisha
H
H
First Department of Pathology, Kansai Medical University, Osaka, Japan.
Nishino
T
T
Kawamura
M
M
Adachi
S
S
Ikehara
S
S
D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000473:pathology*; D006183:H-2 Antigens / Q000276:immunology; D006410:Hematopoiesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011828:Radiation Chimera; D013997:Time Factors
D006183:H-2 Antigens