IgG monoclonal antibodies that inhibit osteoinductivity of human bone matrix-derived proteins (hBMP/NCP).
Monoclonal hBMP/NCP (human bone morphogenetic protein and associated noncollagenous proteins) antibodies of the IgG class were produced. In vitro, 12 of 19 hBMP/NCP antibodies showed functional inhibition of hBMP/NCP-induced chondroneogenesis in a neonatal muscle tissue assay. Inducing factors were characterized by their inhibiting antibodies with immunoblotting. Several peptide factors seem to be involved in the cascade of induced chondro- and osteogenesis.
Kübler
N
N
Department of Oral and Maxillofacial Surgery, University of Würzburg, Germany.
Reuther
J
J
Kirchner
T
T
Pfaff
M
M
Müller-Hermelink
H K
HK
Albert
R
R
Sebald
W
W
D000818:Animals; D000911:Antibodies, Monoclonal; D001857:Bone Matrix / Q000737:chemistry* / Q000502:physiology; D019485:Bone Morphogenetic Proteins; D002356:Cartilage / Q000254:growth & development; D016326:Extracellular Matrix Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry; D006131:Growth Inhibitors / Q000737:chemistry / Q000276:immunology*; D006133:Growth Substances / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis / Q000187:drug effects*; D011506:Proteins / Q000037:antagonists & inhibitors / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D019485:Bone Morphogenetic Proteins; D016326:Extracellular Matrix Proteins; D006131:Growth Inhibitors; D006133:Growth Substances; D007074:Immunoglobulin G; D011506:Proteins