Isolation of the facA (acetyl-CoA synthetase) gene of Phycomyces blakesleeanus.
A 5.6 kb DNA fragment from the fungus Phycomyces blakesleeanus has been cloned and sequenced. The fragment contains a gene that probably codes for the enzyme acetyl-coenzyme A synthetase (facA). The amino acid sequence deduced for the P. blakesleeanus protein is highly homologous to those of acetyl-coA-synthetases from other organisms. When placed under the control of a constitutive promoter from Aspergillus nidulans, the cloned gene complemented a facA- mutation of this organism. In P. blakesleeanus, the expression of facA is induced by acetate.
Garre
V
V
Departamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, Spain.
Murillo
F J
FJ
Torres-Martínez
S
S
D000106:Acetate-CoA Ligase / Q000096:biosynthesis / Q000235:genetics*; D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004790:Enzyme Induction / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D010800:Phycomyces / Q000201:enzymology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000085:Acetates; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000106:Acetate-CoA Ligase