title: Parallel up-regulation of CD11B and CD45 on neutrophilic leukocytes exposed to soluble factors of oral pathogens.
abstract: We tested culture supernatants from a battery of oral bacterial strains for their ability to influence the expression of CD11b and CD45 on the neutrophil plasma membrane. Several bacterial extracts stimulated the up-regulation of both CD11b and CD45 simultaneously. Two supernatants in particular (a clinical isolate of A. actinomycetemcomitans and F. nucleatum ATCC25586) potently stimulated the deployment of CD11b and CD45 from their intracellular storage site to the plasma membrane. Both supernatants inhibited superoxide release stimulated by exposure of neutrophils to formyl methionyl leucyl phenylalanine (FMLP) but had variable effects on superoxide release stimulated by phorbol myristate acetate (PMA). The ability of products of oral bacteria to modulate neutrophil plasma membrane antigen composition may regulate functional reactivity and thus be an important factor in the pathogenesis of periodontal infection and inflammation.
authors.affiliation: Department of Pediatric Dentistry, Indiana University School of Dentistry, Indianapolis.
authors.forename: Y
authors.initials: Y
authors.lastname: Cui
authors.forename: D
authors.initials: D
authors.lastname: English
authors.forename: S
authors.initials: S
authors.lastname: Neel
authors.forename: K
authors.initials: K
authors.lastname: Harvey
authors.forename: R
authors.initials: R
authors.lastname: Siddiqui
authors.forename: L
authors.initials: L
authors.lastname: Akard
authors.forename: J
authors.initials: J
authors.lastname: Jansen
authors.forename: C V
authors.initials: CV
authors.lastname: Hughes
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D001419:Bacteria / Q000276:immunology*; D018845:CD11 Antigens; D005434:Flow Cytometry; D006801:Humans; D017493:Leukocyte Common Antigens / Q000096:biosynthesis* / Q000235:genetics; D009055:Mouth / Q000382:microbiology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D015703:Antigens, CD; D018845:CD11 Antigens; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D017493:Leukocyte Common Antigens; D013755:Tetradecanoylphorbol Acetate