Isolation and sequence determination of porcine class II DRB alleles amplified by PCR.
The first domain exon of a porcine DRB gene was amplified by the polymerase chain reaction (PCR), and the nucleotide sequence was determined. In a material consisting of 10 unrelated animals, five different alleles were identified, all probably belonging to a single locus designated DRB1. In addition, a non-expressed locus, designated DRB2, was coamplified with DRB1. This pseudogene, containing a single base deletion, also exhibited some variation, but at a very restricted level compared with DRB1. In pairwise comparisons of DRB1 alleles, the number of amino acid substitutions ranged between 6 and 21 out of 83 positions compared.
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Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, Oslo.
Olsaker
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Lingaas
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Lie
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D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004247:DNA; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011544:Pseudogenes; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics / Q000276:immunology*
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II; D004247:DNA