In vitro release of [3H]5-hydroxytryptamine from fetal and maternal brain by drugs of abuse.
Cortical synaptosomes were prepared from pregnant dams (GD-17) and rat fetuses (ED-17), loaded with [3H]5-HT and assayed to evaluate release mediated by cocaine (COC), fenfluramine (FEN) and 3,4-methylenedioxymethamphetamine (MDMA). COC and FEN elicited a high-affinity (10(-9) M) release response in fetal tissue which was not apparent in the dam. MDMA-induced release was similar in magnitude in both tissue types. Consequently, the release of 5-HT from developing neurons may be one mechanism by which COC and FEN elicit their teratogenetic effects in utero.
Kramer
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Department of Psychiatry, State University of New York at Stony Brook 11794.
Azmitia
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Whitaker-Azmitia
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D015104:3,4-Methylenedioxyamphetamine / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000196:embryology*; D001923:Brain Chemistry / Q000187:drug effects*; D003042:Cocaine / Q000493:pharmacokinetics / Q000494:pharmacology; D015198:Designer Drugs / Q000493:pharmacokinetics / Q000494:pharmacology; D005260:Female; D005277:Fenfluramine / Q000493:pharmacokinetics / Q000494:pharmacology; D066298:In Vitro Techniques; D008431:Maternal-Fetal Exchange; D018817:N-Methyl-3,4-methylenedioxyamphetamine; D011189:Potassium Chloride / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism*; D019966:Substance-Related Disorders / Q000378:metabolism*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
D015198:Designer Drugs; D005277:Fenfluramine; D012701:Serotonin; D015104:3,4-Methylenedioxyamphetamine; D011189:Potassium Chloride; D003042:Cocaine; D018817:N-Methyl-3,4-methylenedioxyamphetamine