Structures of nodulation factors from the nitrogen-fixing soybean symbiont Rhizobium fredii USDA257.
We have isolated and characterized the extracellular Nod factors of Rhizobium fredii USDA257, a nitrogen-fixing symbiont of soybean [Glycine max (L.) Merr.] and several other legume species. These signals are produced upon exposure to the isoflavone genistein and consist of a series of substituted, beta 1,4-linked tri-, tetra-, and pentamers of N-acetylglucosamine. N-Vaccenic acid replaces acetate on the nonreducing residue, and the reducing residue contains alpha-linked 2-O-methylfucose on carbon 6. Small amounts of a fucose-containing tetramer also were present. The Nod factors elicit root-hair deformations on soybean and two other plants at concentrations ranging from 10(-6) to 10(-12) M.
Bec-Ferté
M P
MP
Laboratoire de Pharmacologie et de Toxicologie Fondamentales du Centre National de la Recherche Scientifique, Toulouse, France.
Krishnan
H B
HB
Promé
D
D
Savagnac
A
A
Pueppke
S G
SG
Promé
J C
JC
D000117:Acetylglucosamine / Q000737:chemistry*; D001681:Biological Assay; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000382:microbiology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D018517:Plant Roots / Q000033:anatomy & histology / Q000502:physiology; D010946:Plants, Medicinal; D012231:Rhizobium / Q000737:chemistry*; D013559:Symbiosis
D009844:Oligosaccharides; D000117:Acetylglucosamine