title: Lack of evidence for increased adherent growth in broth or human serum of clinically significant coagulase-negative staphylococci.
abstract: The relevance of adherent growth of coagulase-negative staphylococci (CNS) in vitro to their clinical significance is controversial. We have investigated the accumulation on polymer surfaces of clinically significant CNS strains, isolated from blood cultures and intravascular catheter tips, compared with controls. The adherent growth of significant CNS isolates in a microtitre tray assay was not greater than controls, and this finding was repeated when different combinations of target polymer surface, culture medium and incubation atmosphere type were investigated. The surface accumulation of CNS cultured in pooled human serum was poor compared with culture in broth. Whole cell and surface protein profiles of serum grown Staphylococcus epidermidis differed markedly from broth cultured cells. We recommend the use of human body fluids, such as serum, as useful culture media to assess whether potential virulence determinants are likely to be expressed in vivo. The microtitre tray assay of adherent growth is not helpful in determining the virulence of individual CNS isolates.
authors.affiliation: Department of Experimental and Clinical Microbiology, University of Sheffield Medical School, UK.
authors.forename: M H
authors.initials: MH
authors.lastname: Wilcox
authors.forename: F
authors.initials: F
authors.lastname: Schumacher-Perdreau
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D001769:Blood; D002473:Cell Wall / Q000737:chemistry; D003030:Coagulase; D003470:Culture Media; D006801:Humans; D007231:Infant, Newborn; D013212:Staphylococcus epidermidis / Q000201:enzymology / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D003030:Coagulase; D003470:Culture Media