title: Chemical synthesis, expression and mutagenesis of a gene encoding beta-cryptogein, an elicitin produced by Phytophthora cryptogea.
abstract: Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining residues. All of the highly necrotic beta-elicitins have a hydrophilic residue (usually lysine) at position 13, whereas in the less necrotic alpha-elicitins this residue is replaced by a valine. Here, we report the synthesis and expression of a gene encoding a highly necrotic elicitin, beta-cryptogein, and we show that the substitution of Lys-13 of this recombinant protein by a valine leads to a drastic alteration to the necrotic activity of the recombinant protein.
authors.affiliation: Laboratoire d'Etude des Protéines INRA, Jony-en-Josas, France.
authors.forename: M J
authors.initials: MJ
authors.lastname: O'Donohue
authors.forename: H
authors.initials: H
authors.lastname: Gousseau
authors.forename: J C
authors.initials: JC
authors.lastname: Huet
authors.forename: D
authors.initials: D
authors.lastname: Tepfer
authors.forename: J C
authors.initials: JC
authors.lastname: Pernollet
mesh_terms: D020418:Algal Proteins; D000595:Amino Acid Sequence; D001483:Base Sequence; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics*; D005813:Genes, Synthetic / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D010838:Phytophthora / Q000235:genetics*; D010947:Plants, Toxic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000187:drug effects
chemical_list: D020418:Algal Proteins; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; C061032:cryptogein protein, Phytophthora cryptogea