Tissue distribution of bacteriochlorin a labelled with 99mTc-pertechnetate in hamster Greene melanoma.
Bacteriochlorin a (BCA), a new photosensitizer for photodynamic therapy, was labelled with 99mTc-pertechnetate following a method for the irreversible coupling of 99mTc-pertechnetate to proteins. Biodistribution studies were conducted in male Syrian Golden hamsters with hamster Greene melanoma implanted s.c. on both sides of the abdomen. After i.v. administration of 99mTc-pertechnetate-labelled BCA 17 tissue and fluid samples were analysed at time intervals ranging from 1 to 24 h. Technetium-labelled BCA showed a pronounced affinity for tissues belonging to the reticuloendothelial system. Peak activities, 1 h post-injection, were distributed as follows: lung, liver, spleen, urine > small intestine, kidney, blood, heart, stomach, large intestine > thyroid, tumour, bone, skin, muscle, eye >> brain. It is concluded that the technetium-labelled photosensitizer BCA does not accumulate selectively in neoplastic tissue.
Schuitmaker
J J
JJ
Department of Ophthalmology, State University of Leiden, The Netherlands.
Feitsma
R I
RI
Journée-De Korver
J G
JG
Dubbelman
T M
TM
Pauwels
E K
EK
D000818:Animals; D006224:Cricetinae; D007553:Isotope Labeling; D008297:Male; D008546:Melanoma, Experimental / Q000378:metabolism*; D009368:Neoplasm Transplantation; D011166:Porphyrins / Q000493:pharmacokinetics*; D013670:Sodium Pertechnetate Tc 99m; D014018:Tissue Distribution
D011166:Porphyrins; C065423:bacteriochlorin a; D013670:Sodium Pertechnetate Tc 99m