title: Rapid identification of the common apo E isoform genotype using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP).
abstract: Polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) was used to identify the common apoprotein E (apo E) isoform genotypes. DNA from the target sequence on apo E was amplified by PCR from peripheral blood genomic DNA. Non-radiolabelled natural PCR products were electrophoresed on a polyacrylamide gradient gel and automatically silver-stained. Bands compatible with each of the three common apo E isoforms were obtained. This method, which does not require restriction enzymes or DNA purification, is a rapid and useful means of detecting the apo E isoform genotype.
authors.affiliation: Genetic Research Laboratory, SRL, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Aozaki
authors.forename: R
authors.initials: R
authors.lastname: Kawaguchi
authors.forename: U
authors.initials: U
authors.lastname: Ogasa
authors.forename: K
authors.initials: K
authors.lastname: Hikiji
authors.forename: N
authors.initials: N
authors.lastname: Kubo
authors.forename: I
authors.initials: I
authors.lastname: Sakurabayashi
mesh_terms: D001057:Apolipoproteins E / Q000235:genetics*; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000032:analysis / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007536:Isomerism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D001057:Apolipoproteins E; D004277:DNA, Single-Stranded