title: [Analogs of 3'-dephospho-CoASH with a phosphodiester bond as substrates of the S-acetylation reaction, catalyzed by acetyl-CoA-synthetase fom rabbit myocardium].
abstract: A number of earlier unknown 3'-dephospho-CoASH analogues with the pyrophosphate fragment replaced by an ester or phosphodiester bond were synthesized and tested in S-acetylation reaction, catalyzed by acetyl-CoA synthetase (EC 6.2.1.1) from rabbit myocardium. 3'-Dephospho-CoASH analogues with a phosphodiester bond, e.g. (Ia), had a lower affinity and diminished kinetic parameters than 3'-dephospho-CoASH (Km = 1 and 0.2 mM, respectively). The adenine substitution in (Ia) by guanine or hypoxanthine (but not cytosine) residue resulted in a loss of substrate properties. 3'-Dephospho-CoASH with an ester bond were not capable of accepting acetate under conditions used and only slightly inhibited the enzymic activity.
authors.forename: A I
authors.initials: AI
authors.lastname: Biriukov
authors.forename: Iu N
authors.initials: IuN
authors.lastname: Zhukov
authors.forename: V M
authors.initials: VM
authors.lastname: Kopelevich
authors.forename: L N
authors.initials: LN
authors.lastname: Bulanova
authors.forename: V I
authors.initials: VI
authors.lastname: Gunar
mesh_terms: D000106:Acetate-CoA Ligase / Q000378:metabolism*; D000107:Acetylation; D000818:Animals; D002384:Catalysis; D003065:Coenzyme A / Q000737:chemistry / Q000378:metabolism*; D004952:Esters / Q000737:chemistry; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D011817:Rabbits; D013379:Substrate Specificity
chemical_list: D004952:Esters; D009943:Organophosphorus Compounds; C015787:dephosphocoenzyme A; D000106:Acetate-CoA Ligase; D003065:Coenzyme A
vernacular_title: Analogi 3'-defosfo-CoASH s fosfodiéfirnoĭ sviaz'iu kak substraty reaktsii S-atsetilirovaniia, kataliziruemoĭ atsetil-CoA-sintetazoĭ miokarda krolika.