Corticotropin release inhibiting factor is encoded within prepro-TRH.
Corticotropin synthesis and secretion is under negative feedback regulation by glucocorticoids. In addition a hypothalamic factor inhibiting ACTH synthesis and secretion, named corticotropin release inhibiting factor (CRIF), has been postulated but not identified. Here we report that transient transfection of a rat prepro-TRH cDNA into the mouse anterior pituitary tumor cell line AtT-20 inhibits the synthesis and secretion of both basal and CRH-stimulated ACTH. Thus, CRIF appears to be encoded by the same precursor as TRH, suggesting a coordinated regulation of pituitary-adrenal and pituitary-thyroid functions.
Redei
E
E
Department of Pharmacology, University of Pennsylvania, Philadelphia 19104.
Hilderbrand
H
H
Aird
F
F
D000324:Adrenocorticotropic Hormone / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D051379:Mice; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D010911:Pituitary Neoplasms; D011498:Protein Precursors / Q000235:genetics*; D013973:Thyrotropin-Releasing Hormone / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D011498:Protein Precursors; C063596:prepro-thyrotropin-releasing hormone; D013973:Thyrotropin-Releasing Hormone; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone