title: Relevance of sequence statistics for the properties of extremophilic proteins.
abstract: The amino acid composition of proteins from mesophilic and extremophilic organisms is commonly assumed to reflect the mechanisms of molecular adaptation to extremes of physical conditions. In this context, halophilic behaviour has been attributed to significantly increased numbers of aspartic and glutamic acid residues. However, extending the analysis to a statistically relevant set of related proteins, dihydrofolate reductase from Halobacterium volcanii, as an example, shows that the increase in negative charge is found to be less significant than other exchanges of amino acids (e.g., Ala, Asn, Arg, Lys, Phe, Ser). Thus, the high water binding capacity of negatively charged residues cannot be unambiguously correlated with the anomalous stability of halophilic proteins. A similar caveat holds for generalizations regarding the thermal stability of proteins. In this case, D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima was compared with a number of mesophilic and moderately thermophilic homologs. Again, 'traffic rules of stabilization', in terms of amino acid changes in going from mesophilic to thermophilic proteins, cannot be given.
authors.affiliation: Institute for Biophysics and Physical Biochemistry, University of Regensburg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Böhm
authors.forename: R
authors.initials: R
authors.lastname: Jaenicke
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis; D004563:Electrochemistry; D004795:Enzyme Stability; D005971:Glutamates / Q000032:analysis; D018698:Glutamic Acid; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology; D006217:Halobacterium / Q000201:enzymology; D006358:Hot Temperature; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics; D013824:Thermus / Q000201:enzymology; D014867:Water / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005971:Glutamates; D011506:Proteins; D014867:Water; D001224:Aspartic Acid; D018698:Glutamic Acid; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D013762:Tetrahydrofolate Dehydrogenase