title: Ontogeny of pituitary adenylate cyclase-activating polypeptide (PACAP) and its binding sites in the rat brain.
abstract: Endogenous levels of pituitary adenylate cyclase-activating polypeptide (PACAP) and its binding site densities were measured in eight brain regions in rats of different ages (2-240 days) by sandwich-enzyme immunoassay and autoradiography. PACAP levels were quite low at day 2, peaked in 30-60 days, and then remained constant in most regions. Such ontogenetic changes are similar to those of vasoactive intestinal polypeptide (VIP) and classical neurotransmitters. PACAP-binding sites were already dense at day 2 and varied only slightly up to day 240. These results suggest that PACAP may have modulatory effects on brain development.
authors.affiliation: Discovery Research Laboratories I, Takeda Chemical Industries, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Masuo
authors.forename: F
authors.initials: F
authors.lastname: Tokito
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: N
authors.initials: N
authors.lastname: Shimamoto
authors.forename: M
authors.initials: M
authors.lastname: Fujino
mesh_terms: D000818:Animals; D001345:Autoradiography; D001665:Binding Sites / Q000502:physiology; D001921:Brain / Q000033:anatomy & histology / Q000254:growth & development*; D001923:Brain Chemistry / Q000502:physiology*; D007124:Immunoenzyme Techniques; D008297:Male; D009479:Neuropeptides / Q000096:biosynthesis* / Q000276:immunology; D018377:Neurotransmitter Agents / Q000096:biosynthesis* / Q000276:immunology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats; D017208:Rats, Wistar; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D011979:Receptors, Pituitary Hormone / Q000096:biosynthesis*
chemical_list: C495403:Adcyap1 protein, rat; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D011979:Receptors, Pituitary Hormone