Glutathione S-transferase and gamma-glutamyl transpeptidase activities in cultured rat hepatocytes treated with tocotrienol and tocopherol.
1. The effect of tocotrienol and tocopherol on glutathione S-transferase (GST) and gamma-glutamyl transpeptidase (GGT) activities in cultured rat hepatocytes were investigated. 2. Tocotrienol and tocopherol significantly decreased GGT activities at 5 days in culture but tocotrienol also significantly decreased GGT activities at 1-2 days. 3. Tocotrienol and tocopherol treatment significantly decreased GST activities at 3 days compared to the control but tocotrienol also decreased GST activities at 1-3 days. 4. Tocotrienol showed a more pronounced effect at a dosage of greater than 50 microM tocotrienol at 1-3 days in culture compared to the control.
Ong
F B
FB
Jabatan Biokimia, Fakulti Perubatan, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala, Lumpur.
Wan Ngah
W Z
WZ
Shamaan
N A
NA
Md Top
A G
AG
Marzuki
A
A
Khalid
A K
AK
D000818:Animals; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D002839:Chromans; D014810:Vitamin E; C013649:plastochromanol 8; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase