Dithranol (anthralin) and 10-butyryl dithranol (butantrone) do not morphologically transform cultured C3H 10T1/2 C18 mouse embryo fibroblasts.
The ability of dithranol and 10-butyryl dithranol to induce morphological cell transformation was studied in cultured C3H 10T1/2 C18 fibroblasts. The cells were incubated with different concentrations of the test compounds for 48 h and cultured for 5 weeks thereafter. At the end of the culture period the cultures were fixed, stained and examined for the presence of transformed foci. Dithranol and 10-butyryl dithranol did not increase the formation of transformed foci, while the positive control compound, 7,12-dimethylbenz(a)anthracene (DMBA), induced a high frequency of transformations significantly different from controls. Thus the in vitro cell transformation model with uninitiated C3H 10T1/2 C18 fibroblasts is not able to detect the weak tumorigenic action of dithranol and 10-butyryl dithranol which has been observed in mouse skin.
Viluksela
M
M
Department of Pharmacology and Toxicology, University of Helsinki, Finland.
Debets
F M
FM
van der Hoeven
J C
JC
Männistö
P T
PT
D000818:Animals; D000875:Anthralin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000187:drug effects / Q000473:pathology*; D005347:Fibroblasts / Q000187:drug effects*; D007509:Irritants / Q000494:pharmacology*; D051379:Mice
D007509:Irritants; C038464:butantrone; D000875:Anthralin