title: Microdialysis sampling coupled on-line to fast microbore liquid chromatography.
abstract: A system which combines intravenous microdialysis sampling on-line with fast microbore liquid chromatographic analysis is described. Using a 14 mm x 1 mm I.D. ODS column, caffeine, theobromine and paraxanthine could be resolved in under 1 min and acetaminophen and its two main metabolites, the glucuronide and sulfate conjugates, could be resolved in 30 s. This fast separation allowed on-line microdialysis sampling to be conducted with a 1-min sampling interval. The system was evaluated by monitoring the pharmacokinetics following intravenous administration of acetaminophen and caffeine.
authors.affiliation: Department of Chemistry, University of Kansas, Lawrence 66045.
authors.forename: A
authors.initials: A
authors.lastname: Chen
authors.forename: C E
authors.initials: CE
authors.lastname: Lunte
mesh_terms: D000082:Acetaminophen / Q000302:isolation & purification / Q000493:pharmacokinetics; D000818:Animals; D002110:Caffeine / Q000302:isolation & purification / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013805:Theobromine / Q000302:isolation & purification; D013806:Theophylline / Q000302:isolation & purification
chemical_list: D000082:Acetaminophen; D002110:Caffeine; D013806:Theophylline; D013805:Theobromine; C021183:1,7-dimethylxanthine