In vitro effects of prostaglandin E1 and indomethacin on mitomycin C-induced sister-chromatid exchanges in mitogen-stimulated human lymphocytes.
In this study, the individual and combined effects of prostaglandin E1 (PGE1) and Indomethacin on mitomycin C (MMC)-induced SCEs in human lymphocytes was investigated in vitro. All MMC-treated cultures showed a great increase of SCEs (approximately two-fold), indicating its ability to induce mutations. SCE data showed that MMC-induced SCEs were reduced significantly in the presence of PGE1 in pooled analysis of six experiments (60.55% reduction of SCEs at 10(-6) M, 34.13% reduction of SCEs at 10(-9) M). In contrast the presence of indomethacin in the medium during MMC treatment of cells failed to show a significant reduction of SCEs in pooled analysis (21.17%). However, individual analyses revealed only two of six donors with a significant SCE response. Thus, the findings suggest that PGE1 can modify the DNA damaging effect of carcinogens and thereby may prevent the initiation of the carcinogenic process.
Ekmekci
A
A
Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Ankara, Turkey.
Sayl
A
A
Dönmez
H
H
Bal
F
F
D000328:Adult; D000527:Alprostadil / Q000494:pharmacology*; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D004357:Drug Synergism; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D016685:Mitomycin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012854:Sister Chromatid Exchange
D016588:Anticarcinogenic Agents; D010835:Phytohemagglutinins; D016685:Mitomycin; D000527:Alprostadil; D007213:Indomethacin