Fimbria-specific immune response in various inbred mice inoculated with Porphyromonas gingivalis 381.
We studied the genetic control of Porphyromonas gingivalis fimbriae response. Inbred mice with different H-2 haplotypes and/or different genetic backgrounds were inoculated with viable P. gingivalis 381 cells and tested for fimbria-specific T cell responses in vivo (delayed-type hypersensitivity). H-2d mice showed a strong footpad response, whereas H-2b mice showed a weak response to fimbriae from P. gingivalis. Similar evidence of genetic control was obtained with an enzyme-linked immunosorbent assay for the detection of IgG antibody in inbred mice (BALB/c, C3H/HeN and C57BL/6). Several immunoglobulin G (IgG) subclass responses were associated with H-2 in these B10 congenic mice. However, quantification of IgG antibody to fimbriae was not controlled by H-2 in B10 congenic mice. The results indicate that, in mice, the responsiveness to fimbriae of P. gingivalis can be controlled by several genes, including the H-2 complex. C3H/HeN mice were inoculated with the fimbriae intravenously, and the expression of surface antigens on spleen T cells was measured in a fluorescent antibody cell sorter. Stimulation by fimbriae resulted in a changed expression of surface antigens on T cells. Thus, the fimbriae can induce T cell activation.
Isogai
E
E
Department of Preventive Dentistry, Higashi Nippon Gakuen University, Ishikari-Tobetsu, Hokkaido, Japan.
Isogai
H
H
Takagi
S
S
Ishii
N
N
Fujii
N
N
Kimura
K
K
Hayashi
M
M
Yoshimura
F
F
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006183:H-2 Antigens / Q000235:genetics; D006239:Haplotypes; D006968:Hypersensitivity, Delayed; D007074:Immunoglobulin G / Q000032:analysis; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains / Q000276:immunology*; D016966:Porphyromonas gingivalis / Q000276:immunology*; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
D000907:Antibodies, Bacterial; D006183:H-2 Antigens; D007074:Immunoglobulin G