Map-based cloning of a protein kinase gene conferring disease resistance in tomato.
The Pto gene in tomato confers resistance to races of Pseudomonas syringae pv. tomato that carry the avirulence gene avrPto. A yeast artificial chromosome clone that spans the Pto region was identified and used to probe a leaf complementary DNA (cDNA) library. A cDNA clone was isolated that represents a gene family, at least six members of which genetically cosegregate with Pto. When susceptible tomato plants were transformed with a cDNA from this family, they were resistant to the pathogen. Analysis of the amino acid sequence revealed similarity to serine-threonine protein kinases, suggesting a role for Pto in a signal transduction pathway.
Martin
G B
GB
Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY 14853-1902.
Brommonschenkel
S H
SH
Chunwongse
J
J
Frary
A
A
Ganal
M W
MW
Spivey
R
R
Wu
T
T
Earle
E D
ED
Tanksley
S D
SD
D000595:Amino Acid Sequence; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins; D012150:Polymorphism, Restriction Fragment Length; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011549:Pseudomonas / Q000472:pathogenicity; D015398:Signal Transduction; D014675:Vegetables / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D014774:Virulence
D018076:DNA, Complementary; D010940:Plant Proteins; C084354:Pto protein, Lycopersicon; D017346:Protein Serine-Threonine Kinases