Quantitative analysis of c-erbB-2 protein in breast cancer tissue by enzyme immunoassay.
The introduction of c-erbB-2 protein to clinical medicine as a prognostic indicator and tumor marker is desired. The authors determined the concentration of c-erbB-2 protein in 81 breast cancer tissues by enzyme immunoassay. The concentration of c-erbB-2 protein in breast cancer tissues showed a broad spectrum. A significant correlation was observed between tissue c-erbB-2 protein concentration and estrogen receptor status or immunohistochemical determination. In patients with distant metastases, there was a close association between c-erbB-2 protein concentration in the primary tumor and serum c-erbB-2 protein level. Cases with a high tumor concentration of c-erbB-2 protein (> 1000 U/mg total protein; 18.5% of cases) had a poor prognosis. From the above, we concluded the measurement of tissue c-erbB-2 protein by enzyme immunoassay to be clinically useful in breast cancer.
Narita
T
T
Department of Surgery II, Nagoya University School of Medicine.
Funahashi
H
H
Satoh
Y
Y
Imai
T
T
Takagi
H
H
D014408:Biomarkers, Tumor / Q000032:analysis* / Q000097:blood; D001940:Breast / Q000737:chemistry / Q000473:pathology; D001943:Breast Neoplasms / Q000097:blood / Q000737:chemistry* / Q000473:pathology; D066246:ErbB Receptors / Q000032:analysis* / Q000097:blood; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000097:blood; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen / Q000032:analysis; D015996:Survival Rate
D014408:Biomarkers, Tumor; D066246:ErbB Receptors; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen