Nucleotide sequence of a gene for nitrite reductase from Arabidopsis thaliana.
A nitrite reductase (NiR) gene was recovered from Arabidopsis thaliana genomic library by the homology with a cDNA of spinach NiR and sequenced. Based on the comparison with the spinach cDNA, the Arabidopsis NiR gene was concluded to contain 4 exons [exon 1 of 376 bp (beginning with ATG start codon), exon 2 of 355 bp, exon 3 of 289 bp and exon 4 of 741 bp (ending at TGA stop codon)] and 3 introns (intron 1 of 196 bp, intron 2 of 81 bp and intron 3 of 77 bp). This conclusion was confirmed by the analysis using the RT-PCR method. The deduced amino acid sequence of the coding region of the Arabidopsis NiR gene had high similarities with those of NiR genes of other plants including spinach.
Tanaka
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T
Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Japan.
Ida
S
S
Irifune
K
K
Oeda
K
K
Morikawa
H
H
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018076:DNA, Complementary; D018744:DNA, Plant; D005091:Exons; D017343:Genes, Plant; D015698:Genomic Library; D007438:Introns; D008969:Molecular Sequence Data; D009572:Nitrite Reductases / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; D009572:Nitrite Reductases