AZT-induction of micronuclei in human lymphocyte subpopulations.
Micronucleus (MN) induction by azidothymidine (AZT) in B and T lymphocytes was analyzed by a recently developed MAC (morphology-antibody-chromosome) method which allows the immunologic identification of different cell lineages. An increased frequency of MN in AZT-treated cultures compared with controls was observed. CD4 cells were found to be more sensitive to AZT damage. AZT-treated cultures showed a significant decrease in the proportion of CD4 interphasic cells. Furthermore, higher MN frequencies in isolated lymphocytes than in whole blood in both control and AZT-treated cultures were observed.
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Departamento de Citogenética, Instituto de Investigaciones Hematológicas Mariano R. Castex, Academia Nacional de Medicina, Buenos Aires, Argentina.
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Larripa
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Slavutsky
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D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D013601:T-Lymphocytes / Q000187:drug effects*; D015215:Zidovudine / Q000633:toxicity*
D015215:Zidovudine