Induction of gamma GT- and GST-P positive foci in the liver of rats treated with 2-nitropropane or propane 2-nitronate.
2-Nitropropane (2-NP) or its anionic form propane 2-nitronate (P2-N) were tested as initiators in a sequential model of rat hepatocarcinogenesis, at the end of which preneoplastic foci were histologically detected. Six intraperitoneal (i.p.) injections of 25, 50 or 100 mg 2-NP or P2-N/kg body weight resulted in the appearance of liver gamma-glutamyltranspeptidase (gamma GT)- and glutathione S-transferase (GST-P)-positive foci, whose number and size increased with the dose of initiator. 2-NP and P2-N were equally effective. The potency of the highest dose (6 x 100 mg/kg body wt) was comparable to that of a single injection of diethylnitrosamine (100 or 200 mg/kg body wt). This work provides a short-term (70 days) and convenient model for further studies on 2-NP carcinogenicity.
Astorg
P
P
Unité de Toxicologie Nutritionnelle, Institut National de la Recherche Agronomique, Dijon, France.
Bergès
R
R
Suschetet
M
M
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D002273:Carcinogens / Q000633:toxicity*; D005982:Glutathione Transferase / Q000032:analysis* / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000201:enzymology / Q000473:pathology; D008297:Male; D009595:Nitroparaffins / Q000633:toxicity*; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000201:enzymology / Q000473:pathology; D011407:Propane / Q000031:analogs & derivatives* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000633:toxicity; D005723:gamma-Glutamyltransferase / Q000032:analysis* / Q000096:biosynthesis
D014408:Biomarkers, Tumor; D002273:Carcinogens; D009595:Nitroparaffins; D012997:Solvents; C069720:propane-2-nitronate; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase; C016617:2-nitropropane; D011407:Propane