Differential distribution of neurocalcin isoforms in rat spinal cord, dorsal root ganglia and muscle spindle.
The cellular distribution of neurocalcin isoforms in rat spinal cord, dorsal root ganglia and muscle spindle was examined using immunohistochemical techniques and two antibodies against different isoforms. In the study using the antibody against the purified neurocalcin from bovine brain, nerve fiber terminals and the cytoplasm of small-sized neurons in the posterior horn, some medium-sized neurons and numerous axons were strongly stained. In dorsal root ganglia, the cytoplasm of various-sized neurons, including about 40% of the total neurons, was also stained. Sensory nerve endings in muscle spindle were immunopositive. When the antibody against the expressed neurocalcin delta was applied, nuclei of the neurons were mainly stained in the spinal cord and dorsal root ganglion. The cytosol of glial cells but not axons in white matter showed neurocalcin delta-like immunoreactivity against the antibody. These findings suggest that each of the differently located isoforms may play a distinct role in Ca2+ signaling in sensory systems.
Okazaki
K
K
Department of Pharmacology, Nagoya University School of Medicine, Japan.
Iino
S
S
Inoue
S
S
Kobayashi
S
S
Hidaka
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D003429:Cross Reactions; D005727:Ganglia, Spinal / Q000737:chemistry*; D015151:Immunoblotting; D008297:Male; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry*; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D051596:Neurocalcin; D051381:Rats; D017208:Rats, Wistar; D044169:Receptors, Calcium-Sensing; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000737:chemistry*
D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D044169:Receptors, Calcium-Sensing; C496690:Vsnl1 protein, rat