Enhancement of CDP-DG:inositol transferase activity in src- and erbB2-transformed cells.
Phosphatidylinositol (PI) synthesis was activated in Rous sarcoma virus-infected NIH3T3 or activated erbB2-transformed NIH3T3 cells. The in vitro activity of CDP-DG:inositol transferase prepared from these cells was also higher than that from normal parent NIH3T3 cells, although phospholipase C and PI kinase activities were not significantly different among these cells. A tyrosine kinase inhibitor, erbstatin, inhibited the PI synthesis in cultured cells, suggesting that Src and ErbB2-associated tyrosine kinases are involved in activation of CDP-DG:inositol transferase in these cell lines.
Imoto
M
M
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Taniguchi
Y
Y
Fujiwara
H
H
Umezawa
K
K
D019870:1-Phosphatidylinositol 4-Kinase; D016475:3T3 Cells; D000818:Animals; D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D016391:Genes, src / Q000235:genetics*; D006873:Hydroquinones / Q000494:pharmacology; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D010716:Phosphatidylinositols / Q000096:biosynthesis; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D018719:Erb-b2 Receptor Tyrosine Kinases; D017855:Transferases (Other Substituted Phosphate Groups) / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism
D019870:1-Phosphatidylinositol 4-Kinase; D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; D006873:Hydroquinones; D009856:Oncogene Proteins, Viral; D010716:Phosphatidylinositols; D017853:Phosphotransferases (Alcohol Group Acceptor); D011505:Protein-Tyrosine Kinases; D018719:Erb-b2 Receptor Tyrosine Kinases; D017855:Transferases (Other Substituted Phosphate Groups); D010738:Type C Phospholipases; C048212:erbstatin