title: Insulin-like growth factor I induced reduction in cysteine proteinase activity in freshly isolated proximal tubule cells of the rat.
abstract: The potential effects of insulin-like growth factor I (IGF-I) on lysosomal cysteine proteinases (cathepsin B, H and L+B activities) were investigated in the freshly isolated proximal tubule cells of rats. IGF-I significantly inhibited these enzyme activities after an incubation time of 80 min. This effect was associated with a dose-dependent increase in cellular protein content. The study suggests that, besides the established enhanced protein synthesis, IGF-I-induced cellular hypertrophy is mediated by a suppression of the proteolytic enzyme activity in proximal tubular cells.
authors.affiliation: Department of Nephrology, University of Würzburg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Ling
authors.forename: S
authors.initials: S
authors.lastname: Vamvakas
authors.forename: L
authors.initials: L
authors.lastname: Schaefer
authors.forename: R M
authors.initials: RM
authors.lastname: Schaefer
authors.forename: M
authors.initials: M
authors.lastname: Teschner
authors.forename: L
authors.initials: L
authors.lastname: Schramm
authors.forename: A
authors.initials: A
authors.lastname: Heidland
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000187:drug effects* / Q000378:metabolism*; D006984:Hypertrophy / Q000201:enzymology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000201:enzymology* / Q000473:pathology; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D003546:Cysteine Endopeptidases