Identification of Agrobacterium strains by PCR-RFLP analysis of pTi and chromosomal regions.
Chromosomes and Ti plasmids of 41 Agrobacterium strains, belonging to biovars 1, 2, 3, and Agrobacterium rubi species were characterized by the restriction fragment length polymorphism of PCR-amplified DNAs. Profiles that were obtained by the analysis of the amplified 16S rDNA confirmed the grouping of the strains according to their species. Higher polymorphism was detected in the intergenic spacer between the 16S rDNA and 23S rDNA genes, allowing efficient discrimination of strains. Identification of most strains was possible, and the genetic relatednesses of Agrobacterium strains could be estimated. The analysis of the plasmid Ti encoded regions between the tmr and nos genes, and the virA and virB2 genes, allowed fingerprinting of Ti plasmids. Genomic typing by the rapid PCR-RFLP method is thus shown to be useful for an independent identification of strains and of the conjugative Ti plasmids.
Ponsonnet
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Laboratoire d'Ecologie Microbienne des Sols. Centre National de la Recherche Scientifique, URA 1450, Université Claude Bernard Lyon 1, Villeurbanne, France.
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D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D017931:DNA Primers; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D017931:DNA Primers; C008666:nopaline; D001120:Arginine