Rapid assay of the anti-inflammatory activity of topical corticosteroids by inhibition of a UVA-induced neutrophil infiltration in hairless mouse skin. I. The assay and its sensitivity.
A single large dose of UVA induced an intense infiltration of neutrophils into the lower dermis of hairless mouse skin, peaking at 24 h. The ability of 15 name brand topical corticosteroids to suppress this infiltrate was determined. The rank order of suppression correlated with the accepted clinical category of anti-inflammatory potency. This is a rapid screening procedure for assaying the anti-inflammatory activity of new steroids and for optimizing the vehicle.
Woodbury
R A
RA
University of Pennsylvania School of Medicine, Department of Dermatology, Philadelphia.
Kligman
L H
LH
Woodbury
M J
MJ
Kligman
A M
AM
D000287:Administration, Topical; D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002465:Cell Movement; D004307:Dose-Response Relationship, Radiation; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D009504:Neutrophils / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D013997:Time Factors; D014466:Ultraviolet Rays
D000305:Adrenal Cortex Hormones; D000893:Anti-Inflammatory Agents