title: A cloned lymphoid Thy1+ tumor line derived from murine yolk sac cells maintained in long-term cell culture in the absence of a thymic microenvironment expresses an unusual cell surface phenotype.
abstract: We have previously established conditions under which murine yolk sac cells can be maintained in vitro in the absence of thymus, and we reported that a spontaneously transformed Thy1.2+ yolk sac cell line was obtained after long term culture in vitro. We now provide further phenotypic characterization of a clone, YSA1, of this Thy1.2+ cell line showing that it expresses Thy1.2, CD3/TCR V beta 8, IL-2 receptor (IL2R), and heat stable antigen (HSAg), but does not express CD4/CD8 or TCR gamma delta. The YSA1 clone is an immature cell as indicated by the expression of IL2R and HSAg and by nonproduction of IL-2 upon stimulation by anti-CD3 antibody. The data show that yolk sac stem cells can differentiate into CD3/TCR expressing cells in the absence of thymus, but do not progress in vitro paralleling observations made on freshly-isolated yolk sac stem cells.
authors.affiliation: Center for Developmental Biology, University of Wisconsin, Madison.
authors.forename: C P
authors.initials: CP
authors.lastname: Liu
authors.forename: A
authors.initials: A
authors.lastname: Globerson
authors.forename: R
authors.initials: R
authors.lastname: Auerbach
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010641:Phenotype; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000502:physiology*; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000502:physiology*; D015017:Yolk Sac / Q000166:cytology*
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D015375:Receptors, Interleukin-2; D018800:Thy-1 Antigens