Bacterial oxidative stress substance spontaneously recyclizes to form 2-methylbutane-1,2,3,4-tetraol-1,2-cyclophospho-4-phosphate.
The cells of Corynebacterium (Brevibacterium) ammonia-genes cultivated in a medium supplemented with diquat or benzylviologen accumulate 2-methylbutane-1,2,3,4-tetraol-2,4- cyclopyrophosphate as revealed by 31P-NMR spectroscopy. On heating at 120 degrees C for 30 min the cells still maintain a substantial portion of this compound and acquire new cyclic phosphates characterized by 31P-NMR chemical shifts of +17.3 and +20 p.p.m. The +17.3 p.p.m. component was isolated from the preparation of the purified cyclopyrophosphate kept for some time at pH above 7 and it was shown to be 2-methylbutane-1,2,3,4-tetraol-1,2,- cyclophospho-4-phosphate on the grounds of two-dimensional NMR spectroscopy.
Ostrovsky
D
D
Bakh Institute of Biochemistry, Moscow, Russia.
Diomina
G
G
Shipanova
I
I
Sibeldina
L
L
Shashkov
A
A
D001594:Benzyl Viologen / Q000494:pharmacology*; D003352:Corynebacterium / Q000187:drug effects / Q000378:metabolism*; D004178:Diquat / Q000494:pharmacology*; D004896:Erythritol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D018384:Oxidative Stress
D001594:Benzyl Viologen; C073443:2-methyl-butan-1,2,3,4-tetraol-2,4-cyclopyrophosphate; D004178:Diquat; D004896:Erythritol