Active transport of benzo[a]pyrene in apical membrane vesicles from normal human intestinal epithelium.
Transport of the carcinogen benzo[a]pyrene in apical membrane vesicles (AMV) from normal human intestine, was investigated. Benzo[a]pyrene transport was found in AMV throughout the small intestine, but was greatest in colon. Evidence suggesting involvement of P-Glycoprotein (P-Gp), included (1) comparable transport of P-Gp substrate doxorubicin, (2) transport stimulation by ATP and (3) transport suppression by the P-Gp inhibitor, verapamil.
Penny
J I
JI
Department of Surgery, Ninewells Hospital and Medical School, University of Dundee, Scotland, UK.
Campbell
F C
FC
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000032:analysis; D000626:Aminopeptidases / Q000032:analysis; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D018826:CD13 Antigens; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003106:Colon / Q000378:metabolism* / Q000648:ultrastructure; D004317:Doxorubicin / Q000378:metabolism; D005260:Female; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D014700:Verapamil / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D001564:Benzo(a)pyrene; D004317:Doxorubicin; D000255:Adenosine Triphosphate; D014700:Verapamil; D000469:Alkaline Phosphatase; D000626:Aminopeptidases; D018826:CD13 Antigens