title: [Chromosome fractionation in a saccharose density gradient for subsequent flow sorting].
abstract: The human chromosomes were obtained from lymphoblastoid cell line BOLD with normal karyotype, and separated on the basis of their size by velocity sedimentation at 190 g in sucrose gradient. Different chromosomal fractions were stained by applying fluorochromes with different DNA-base specificity: Hoechst 33258 and Olivomycine and were analysed using dual laser cell-sorter ATC-3000 (Brucker). Velocity sedimentation allowed to enrich certain fractions by individual chromosomes and thus speed up the sorting rate from 90 up to 200 chromosomes per second.
authors.forename: A N
authors.initials: AN
authors.lastname: Shatrova
authors.forename: N D
authors.initials: ND
authors.lastname: Aksenov
authors.forename: L V
authors.initials: LV
authors.lastname: Teslenko
authors.forename: V V
authors.initials: VV
authors.lastname: Zenin
mesh_terms: D002458:Cell Fractionation / Q000379:methods*; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods; D002877:Chromosomes, Human / Q000648:ultrastructure*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007834:Lasers; D008677:Metaphase
vernacular_title: Fraktsionirovanie khromosom v gradiente plotnosti sakharozy dlia posleduiushcheĭ protochnoĭ sortirovki.