title: Identification and characterization of phosphoenolpyruvate:fructose phosphotransferase systems in three Streptomyces species.
abstract: Streptomyces lividans, S. coelicolor and S. griseofuscus were examined for the presence of the enzymes of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). All three species were shown to possess Enzyme I, HPr and fructose-specific Enzyme II (IIFru) activities. In S. lividans and S. coelicolor, all three PTS enzymes were fructose-inducible, but in S. griseofuscus the system was expressed constitutively. These organisms apparently lack the HPr(Ser) kinase and HPr(Ser-P) phosphatase that characterize low-GC Gram-positive bacteria.
authors.affiliation: Department of Biology, University of California at San Diego, La Jolla 92093-0116.
authors.forename: F
authors.initials: F
authors.lastname: Titgemeyer
authors.forename: J
authors.initials: J
authors.lastname: Walkenhorst
authors.forename: J
authors.initials: J
authors.lastname: Reizer
authors.forename: M H
authors.initials: MH
authors.lastname: Stuiver
authors.forename: X
authors.initials: X
authors.lastname: Cui
authors.forename: M H
authors.initials: MH
authors.lastname: Saier
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology; D005632:Fructose / Q000378:metabolism; D005816:Genetic Complementation Test; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010766:Phosphorylation; D013045:Species Specificity; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
chemical_list: D005632:Fructose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System