title: Removal of beta III isotype enhances taxol induced microtubule assembly.
abstract: The interaction of beta III-depleted tubulin with taxol was investigated. A monoclonal antibody against the beta III tubulin isotype was immobilized on a sepharose 4B column and used to remove the beta III tubulin isotype from unfractionated tubulin. The assembly of beta III-depleted tubulin in the presence of taxol was enhanced compared to unfractionated tubulin. The critical concentration of unfractionated tubulin in the presence of 10 microM taxol is 0.4 mg/ml, while the critical concentration of beta III-depleted tubulin is 0.16 mg/ml. At different concentration of taxol, the assembly of beta III-depleted tubulin is increased relative to that of unfractionated tubulin and reaches the maximum at about a 1:1 ratio of tubulin and taxol. The assembly of unfractionated tubulin and beta III-depleted tubulin has also been studied by electron microscopy. After 2 minutes at 37 degrees C, unfractionated tubulin assembly in the presence of 10 microM taxol results only in ribbon-like and ring structures; there are no visible microtubules. By 5 minutes, microtubules appear and increase in length. The assembly of beta III-depleted tubulin in the presence of 10 microM taxol occurs more quickly. In contrast to the case with unfractionated tubulin, beta III-depleted tubulin assembles within 2 minutes into microtubules which increase in length with time. At 30 minutes, microtubules assembled from beta III-depleted tubulin are shorter than the microtubules assembled from unfractionated tubulin. There is no visible difference between the microtubules assembled from unfractionated tubulin and beta III-depleted tubulin. Taxol-induced beta III-depleted tubulin assembly is more resistant to the inhibiting effect of podophyllotoxin and colchicine. It is also less sensitive to the inhibiting effect of cold temperature.
authors.affiliation: Department of biochemistry, University of Texas Health Science Center at San Antonio 78284-7760.
authors.forename: Q
authors.initials: Q
authors.lastname: Lu
authors.forename: R F
authors.initials: RF
authors.lastname: Luduena
mesh_terms: D000818:Animals; D001921:Brain; D002417:Cattle; D003078:Colchicine / Q000494:pharmacology; D003080:Cold Temperature; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D017239:Paclitaxel / Q000494:pharmacology*; D011034:Podophyllotoxin / Q000494:pharmacology; D014404:Tubulin / Q000187:drug effects* / Q000378:metabolism
chemical_list: D014404:Tubulin; D011034:Podophyllotoxin; D017239:Paclitaxel; D003078:Colchicine