Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD4 expression.
Lymphocyte chemoattractant factor (LCF) is a lymphocyte cell product that stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. In concert with its chemoattractant activity, LCF induces human T-lymphocyte expression of interleukin 2 receptor. Here we describe the molecular cloning of cDNA encoding human LCF. It is a novel interleukin with no significant homology to any previously described cytokine families. There is an absolute requirement for both autoaggregation of LCF monomers and for membrane-expressed CD4 molecules for LCF-induced migration in lymphocytes.
Cruikshank
W W
WW
Pulmonary Center, Boston University School of Medicine, MA 02118.
Center
D M
DM
Nisar
N
N
Wu
M
M
Natke
B
B
Theodore
A C
AC
Kornfeld
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D015704:CD4 Antigens / Q000096:biosynthesis*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D002460:Cell Line; D002630:Chemotactic Factors / Q000235:genetics / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D006825:Hybridomas; D019410:Interleukin-16; D008222:Lymphokines / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data
D015704:CD4 Antigens; D002630:Chemotactic Factors; D019410:Interleukin-16; D008222:Lymphokines; D004247:DNA