title: Salmonella typhimurium induces expression of P glycoprotein (multidrug resistance 1 gene product) in a promonocytic cell line chronically infected with human immunodeficiency virus type 1.
abstract: This investigation showed that phagocytosis of virulent Salmonella typhimurium by promonocytic leukemia cell line U1, which contains human immunodeficiency virus type 1 (HIV-1) provirus but produces minimal or no virus, and not by uninfected U937 cell line resulted in expression of a functional P glycoprotein. Anti-tumor necrosis factor-alpha (TNF alpha) monoclonal antibody failed to inhibit S. typhimurium-induced P glycoprotein expression. Furthermore, recombinant TNF alpha had no effect on the induction of P glycoprotein expression in U1 cells. These data demonstrate that phagocytosis of virulent S. typhimurium results in an induction of P glycoprotein in association with HIV-1 infection; however, TNF alpha does not appear to play a significant role. Thus, secondary microbial infection in HIV-1-positive persons may play a role in multidrug resistance against antiviral and other antimicrobial agents by an induction of P glycoprotein.
authors.affiliation: Division of Basic and Clinical Immunology, University of California, Irvine.
authors.forename: A
authors.initials: A
authors.lastname: Andreana
authors.forename: S
authors.initials: S
authors.lastname: Gollapudi
authors.forename: S
authors.initials: S
authors.lastname: Gupta
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D004352:Drug Resistance, Microbial / Q000235:genetics; D005786:Gene Expression Regulation; D015497:HIV-1 / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D010587:Phagocytosis; D012486:Salmonella typhimurium / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology; D014774:Virulence
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D014409:Tumor Necrosis Factor-alpha