title: Characterization of the zona pellucida glycoproteins from bovine ovarian and fertilized eggs.
abstract: Bovine zona pellucida (ZP) glycoproteins from ovarian egg emerged as three bands with molecular mass of 78 kDa, 64 kDa and 21 kDa in SDS-PAGE under reducing conditions. Endo-beta-galactosidase (E beta G) digestion of the glycoproteins yielded five products with molecular mass of 76 kDa (E beta G-76), 68 kDa (E beta G-68), 63 kDa (E beta G-63), 47 kDa (E beta G-47) and 21 kDa (E beta G-21) under the same conditions. The N-terminal amino acid sequences of E beta G-76 and E beta G-21 were identical. This fact together with the results of diagonal SDS-PAGE indicated that E beta G-21 (N-terminal region) is linked to E beta G-63 (C-terminal region) through disulfide bond to form E beta G-76. Immunoblot analysis using anti-pig ZP protein antibodies revealed that bovine E beta G-76, E beta G-68 and E beta G-47 correspond to pig PZP2, PZP3 alpha and PZP3 beta glycoproteins, respectively. The E beta G-76 and E beta G-68 components were shown to be specifically cleaved during fertilization.
authors.affiliation: Graduate School of Science and Technology, Chiba University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Noguchi
authors.forename: N
authors.initials: N
authors.lastname: Yonezawa
authors.forename: T
authors.initials: T
authors.lastname: Katsumata
authors.forename: K
authors.initials: K
authors.lastname: Hashizume
authors.forename: M
authors.initials: M
authors.lastname: Kuwayama
authors.forename: S
authors.initials: S
authors.lastname: Hamano
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Nakano
mesh_terms: D000581:Amidohydrolases; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D005306:Fertilization; D006023:Glycoproteins / Q000032:analysis*; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D010063:Ovum / Q000737:chemistry*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D015044:Zona Pellucida / Q000737:chemistry*; D001616:beta-Galactosidase
chemical_list: D000596:Amino Acids; D006023:Glycoproteins; D009005:Monosaccharides; D001616:beta-Galactosidase; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase