title: Presence of gelatinase A and metalloelastase type protease at the plasma membrane of human skin fibroblasts. Influence of cytokines and growth factors on cell-associated metalloendopeptidase levels.
abstract: Gelatinase A and elastase type proteinase (Homsy, et al., 1988) present at plasma membranes of human skin fibroblasts (HSF) were separated by anion exchange chromatography on a DEAE Tris acryl M column. Elastase type proteinase (HSFE1) was able to convert 72 kDa progelatinase A to a lower 66 kDa M.W. active enzyme. Several cytokines (IL-1 beta, IL4, IL6), interferon gamma (IFN gamma) and tumor growth factor beta (TGF-beta) were studied for their ability to modify the levels of those plasma membrane associated proteinases. Among these mediators, only IL-1 beta was found to enhance the amounts of HSF membrane-bound HSFE1 and Gelatinase A.
authors.affiliation: Laboratoire de biologie du tissu conjonctif, ER 74 CNRS, Faculté de Médecine, Université Paris XII, Créteil, France.
authors.forename: J Y
authors.initials: JY
authors.lastname: Beranger
authors.forename: G
authors.initials: G
authors.lastname: Godeau
authors.forename: C
authors.initials: C
authors.lastname: Frances
authors.forename: L
authors.initials: L
authors.lastname: Robert
authors.forename: W
authors.initials: W
authors.lastname: Hornebeck
mesh_terms: D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002478:Cells, Cultured; D002648:Child; D002852:Chromatography, Ion Exchange; D016207:Cytokines / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D018093:Gelatinases / Q000032:analysis*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000032:analysis*; D008970:Molecular Weight; D010196:Pancreatic Elastase / Q000032:analysis*; D012867:Skin / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D016207:Cytokines; D006133:Growth Substances; D007375:Interleukin-1; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D015847:Interleukin-4; D007371:Interferon-gamma; D010196:Pancreatic Elastase; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2