[The effect of metabolic inhibitors on resistance of mannose-specific contacts of Escherichia coli K12 and human neutrophils].
Human neutrophil aggregation induced by Escherichia coli K12 cells has been studied. D-mannose has been found to inhibit the process in the dose-dependent way causing the full blockage at 10 mM concentration. The process of disaggregation induced by the concentration is significantly higher at 10 degrees C than at 37 degrees C. The inhibitors of cellular oxygenation such as N-ethylmaleimide, nordihydroguaiaretic acid, trifluoperazine, and colchicine did not affect the aggregation process. Cellular aggregation was concomitant with production of H2O2, and this reaction was also blocked by D-mannose and depended on the number of bacteria present. The nordihydroguaiaretic acid and N-ethylmaleimide inhibited production of H2O2, while sodium azide enhanced the process. The results show the mannose-specific contact of bacteria and neutrophils to depend on the functional activity of phagocytes including their ability to produce H2O2.
Timoshenko
A V
AV
Fomichev
A Iu
AIu
Cherenkevich
S N
SN
D002449:Cell Aggregation; D003078:Colchicine / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008358:Mannose / Q000378:metabolism*; D009637:Masoprocol / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D014268:Trifluoperazine / Q000494:pharmacology
D014268:Trifluoperazine; D009637:Masoprocol; D006861:Hydrogen Peroxide; D005033:Ethylmaleimide; D008358:Mannose; D003078:Colchicine
Vliianie metabolicheskikh ingibitorov na ustoĭchivost' mannozospetsificheskikh kontaktov Escherichia coli K12 i neĭtrofilov cheloveka.