Co-localization of GABA- and tyrosine hydroxylase-like immunoreactivities in amacrine cells of the rabbit retina.
Previous studies have shown that many of the tyrosine hydroxylase-immunoreactive (TH-IR) amacrine cells in the retina of mammals also show GABA-like immunoreactivity (GABA-IR). However such co-localization has yet to be demonstrated in the rabbit retina. In this study the proportion of TH-IR amacrine cells that show GABA-IR has been determined in the retina of the cat, rat and rabbit following fixation with 4% paraformaldehyde plus varying concentrations of glutaraldehyde. In the retina of the cat and rat, most of the TH-IR amacrine cells showed GABA-IR following fixation using low concentrations (0.01 or 0.1%) of glutaraldehyde. However in the rabbit retina very few of the TH-IR amacrine cells showed GABA-IR unless the glutaraldehyde concentration in the fixative was 0.5% or greater. The results suggest that the dopaminergic amacrine cells in the rabbit retina contain only very low levels of GABA, which are close to the detection limit of the antibody.
Young
H M
HM
Department of Physiology and Pharmacology, University of Queensland, Australia.
D000818:Animals; D000941:Antigens / Q000032:analysis; D002415:Cats; D005976:Glutaral; D007150:Immunohistochemistry; D011817:Rabbits; D051381:Rats; D012160:Retina / Q000737:chemistry* / Q000166:cytology; D016707:Tissue Fixation; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis* / Q000276:immunology; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000276:immunology
D000941:Antigens; D005680:gamma-Aminobutyric Acid; D014446:Tyrosine 3-Monooxygenase; D005976:Glutaral