Actions of agonists and convulsant antagonists on a Drosophila melanogaster GABA receptor (Rdl) homo-oligomer expressed in Xenopus oocytes.
Cytoplasmic injection of Xenopus oocytes with cDNA-derived RNA (cRNA) encoding a wild-type Drosophila melanogaster GABA receptor subunit (Rdl) resulted in functional expression of a GABA receptor homo-oligomer. Membrane currents recorded in response to muscimol, trans (TACA)- and cis (CACA)-4-aminocrotonic acid reversed at membrane potentials close to ECl- and were insensitive to 1.0 x 10(-4) M bicuculline methiodide. An order of potency of GABA approximately muscimol approximately TACA > CACA > glycine was observed. Response of the expressed Drosophila Rdl receptor to GABA was reduced by t-butylbicyclophosphorothionate (TBPS), 4-n-propyl-4'-ethynylbicycloorthobenzoate (EBOB), picrotoxinin and the novel insecticide, fipronil (all at 1.0 x 10(-5) M).
Buckingham
S D
SD
Babraham Institute Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, UK.
Hosie
A M
AM
Roush
R L
RL
Sattelle
D B
DB
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D004594:Electrophysiology; D005260:Female; D015870:Gene Expression; D009865:Oocytes / Q000378:metabolism*; D018079:Receptors, GABA / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014981:Xenopus / Q000378:metabolism*
D000927:Anticonvulsants; D018079:Receptors, GABA