HIV-1 gp41 enhances major histocompatibility complex class I and ICAM-1 expression on H9 and U937 cells.
Based on our findings that HIV-1 (human immunodeficiency virus type 1) soluble gp41 (sgp41; amino acids 539-684) bound to human T, B, and monocyte cells and enhanced major histocompatibility complex (MHC) class I and II antigen expression on Raji cells, we examined the effect of HIV-1 sgp41 on the surface expression of MHC I and II, ICAM-1, and CD4 molecules on human H9 and U937 cells. Flow cytometry (FACS) analysis demonstrated that sgp41 selectively enhanced MHC class I expression by about 75% on H9 cells and by about 85% on U937 cells, while the ICAM-1 expression was increased by about 70% only on H9 cells and remained unaltered on U937 cells; other molecules, such as MHC class II and CD4, remained unaltered. By comparison, alpha-, beta-, and omega-interferons, but not gamma-interferon, showed similar effects as sgp41 on the expression of MHC class I and ICAM-1 on H9 and U937 cells. The results suggest that HIV-1 gp41 may have a biological function that is involved in the regulation of human MHC class I and ICAM-1 expression.
Chen
Y H
YH
Ludwig Boltzmann Institute for AIDS Research, Innsbruck, Austria.
Böck
G
G
Vornhagen
R
R
Steindl
F
F
Katinger
H
H
Dierich
M P
MP
D015703:Antigens, CD / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D005434:Flow Cytometry; D015700:HIV Envelope Protein gp41 / Q000494:pharmacology*; D015497:HIV-1; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D016898:Interferon-alpha / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D008285:Major Histocompatibility Complex / Q000187:drug effects*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism*; D011994:Recombinant Proteins
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D015700:HIV Envelope Protein gp41; D015395:Histocompatibility Antigens Class I; D016898:Interferon-alpha; D011991:Receptors, Virus; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D016899:Interferon-beta; D007371:Interferon-gamma