Postnatal development of the suprachiasmatic nucleus in the rat. Morpho-functional characteristics and time course of tyrosine hydroxylase immunopositive fibers.
According to earlier data, the suprachiasmatic nucleus of neonatal rats is highly innervated by serotonin- and tyrosine hydroxylase-immunopositive fibers [Ugrumov M. V. (1992) Zool. Sci. (Tokyo) 9, 37-45], while the latter were no longer observed in adults. This study has attempted to evaluate the timing of the innervation of the rat suprachiasmatic nucleus by tyrosine hydroxylase-immunopositive fibers, as well as to specify some morpho-functional characteristics of these fibers. According to our semi-quantitative light microscopic immunocytochemical data, few tyrosine hydroxylase-immunopositive fibers were observed in the suprachiasmatic nucleus as early as the second postnatal day. They highly increased in number neonatally reaching a maximum at the 10th postnatal day, and then decreased dramatically in adulthood. These data suggest either the provisional character of the tyrosine hydroxylase-immunopositive fibers themselves or the transient expression of tyrosine hydroxylase within permanent fibers. The tyrosine hydroxylase immunopositivity in the fibers points to their catecholaminergic nature, while the overlapping in the distribution of tyrosine hydroxylase- and serotonin-immunopositive fibers might also suggest the transient expression of tyrosine hydroxylase in serotoninergic neurons. In order to check this hypothesis, the neurotoxins of catecholamine- and serotoninergic neurons, 6-hydroxydopamine and 5,7-dihydroxytryptamine, were intraventricularly injected at the second postnatal day, while their effects were specified by the semi-quantitative immunocytochemistry eight days later. 6-Hydroxydopamine did not modify the content of tyrosine hydroxylase-immunopositive fibers in the suprachiasmatic nucleus. Conversely, the treatment with 5,7-dihydroxytryptamine resulted in a significant increase in the number of the tyrosine hydroxylase-immunopositive fibers, while reducing the amount of the serotoninergic ones.(ABSTRACT TRUNCATED AT 250 WORDS)
Beltramo
M
M
Département de Neurobiologie des Signaux Intercellulaires, CNRS URA 1488, Université P. et M. Curie, Paris, France.
Calas
A
A
Chernigovskaya
E
E
Borisova
N
N
Polenova
O
O
Tillet
Y
Y
Thibault
J
J
Ugrumov
M
M
D015116:5,7-Dihydroxytryptamine / Q000494:pharmacology; D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development* / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D007267:Injections; D008297:Male; D009412:Nerve Fibers / Q000378:metabolism; D016627:Oxidopamine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000254:growth & development* / Q000378:metabolism*; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D015116:5,7-Dihydroxytryptamine; D012701:Serotonin; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase