Radioimmunolocalization of human brain tumor: fundamental studies with indium-111 labeled monoclonal antibody G-22.
Monoclonal antibody (MCA) G-22 reacts selectively with human glioblastoma. Whole immunoglobulin G (IgG) and the F(ab')2 fragment of G-22 were labeled with indium-111, and injected into athymic nude mice bearing xenografts of a human glioblastoma cell line (U-251-MG). Radiolabeled G-22 retained its antigen-binding activity allowing clear visualization of transplanted tumors. Although the 111In-labeled F(ab')2 fragment had a higher tumor-to-tissue ratio than whole IgG, tumor concentration of 111In-labeled G-22 MCA was higher in the latter and it provided much better images for a longer time when visualized by gamma-scintigraphy. These results suggest that 111In-labeled G-22 may be a useful agent for brain tumor imaging.
Wakabayashi
T
T
Department of Neurosurgery, School of Medicine, Nagoya University.
Yoshida
J
J
Mizuno
M
M
Sugita
K
K
Itoh
K
K
Tadokoro
M
M
Oshima
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D001932:Brain Neoplasms / Q000000981:diagnostic imaging*; D005260:Female; D005909:Glioblastoma / Q000000981:diagnostic imaging*; D006801:Humans; D007205:Indium Radioisotopes; D051379:Mice; D008819:Mice, Nude; D016719:Radioimmunodetection; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes