Analysis of proliferative activity in renal lesions induced by N-nitrosobis(2-oxopropyl)amine (BOP) in male Syrian golden hamsters.
Binding of a specific antibody to proliferating cell nuclear antigen (PCNA) and staining of argyrophilic proteins associated with nucleolar organizer regions (AgNORs) were investigated in proliferative lesions induced by N-nitrosobis(2-oxopropyl)amine (BOP) in the hamster kidney. Thirty male Syrian golden hamsters were given three weekly s.c. injections of BOP (10 mg/kg body wt.) and sacrificed for characterization of proliferative changes 30 weeks after the first BOP treatment. Morphologically, lesions of the tubular epithelia were classified either as tubular adenoma or dysplasia, the latter being further classified into small cluster, acidophilic cell, clear cell and cystic types. Immunohistochemistry for PCNA revealed significant increases of cell proliferation activity in adenomas and acidophilic cell types of dysplasia, along with significantly elevated mean numbers of AgNORs per nucleus. The results thus indicate that the acidophilic cell type of dysplasia may be of prime significance as the preneoplastic renal lesion induced by BOP.
Sato
M
M
Division of Pathology, National Institute of Health Sciences, Tokyo, Japan.
Furukawa
F
F
Nishikawa
A
A
Mitsumori
K
K
Imazawa
T
T
Takahashi
M
M
D000236:Adenoma / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D006224:Cricetinae; D007668:Kidney / Q000187:drug effects / Q000473:pathology*; D007680:Kidney Neoplasms / Q000139:chemically induced / Q000473:pathology*; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000473:pathology; D008297:Male; D008647:Mesocricetus; D008940:Mitotic Index / Q000187:drug effects; D009363:Neoplasm Proteins / Q000032:analysis; D009602:Nitrosamines / Q000633:toxicity*; D009687:Nuclear Proteins / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen
D002273:Carcinogens; D009363:Neoplasm Proteins; D009602:Nitrosamines; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; C013622:nitrosobis(2-oxopropyl)amine