Isolation and mapping of a cosmid clone containing the human NAT2 gene.
The NAT2 gene encodes for a polymorphic arylalkylamine N-acetyltransferase and thus accounts for the human N-acetylation polymorphism. By a NAT2-specific primer set we have screened a human chromosome 8-specific cosmid library. A positive cosmid clone was mapped by fluorescence in situ hybridization to 8p22. The polymerase chain reaction followed by restriction analysis of the PCR product was used to identify allele 2 to be contained in the cosmid clone.
Franke
S
S
Center for Human Genetics and Genetic Counselling, University of Bremen, Germany.
Klawitz
I
I
Schnakenberg
E
E
Rommel
B
B
Van de Ven
W
W
Bullerdiek
J
J
Schloot
W
W
D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D003360:Cosmids; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length
D017931:DNA Primers; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human