title: Localisation of receptors for prolactin in ovine skin.
abstract: Although prolactin (PRL) receptors are found in many mammalian tissues, specific PRL binding to mammalian skin has not been demonstrated. In view of the temporal relationships observed between photoperiod, circulating PRL and pelage replacement in seasonally responsive mammals, we sought to provide evidence of PRL receptors in ovine skin. Cryosections of skin from New Zealand Wiltshire ewes were incubated with radiolabelled human GH (125I-hGH) and ovine PRL (125I-oPRL) in the presence and absence of excess unlabelled hormones (hGH, oPRL or ovine GH (oGH)). Binding was inhibited by unlabelled oPRL and hGH but not by oGH. In microautoradiographs, both radioligands were localised most strongly in the dermal papillae of wool follicles in the anagen (growth) phase of the hair cycle and in apocrine sweat glands. Higher levels of specific binding to dermal papilla cells, compared with the follicle epithelial matrix and the surrounding dermis, were confirmed by measurement of microautoradiograph silver grain density (respectively, 34.1 +/- 3.0, 11.4 +/- 1.0 and 5.5 +/- 0.5 grains per 100 microns2 (mean +/- S.E.M., n = 10)). Total binding for 125I-hGH and 125I-oPRL radioligands to follicle dermal papilla was not significantly different (34.1 +/- 3.0 vs 43.6 +/- 2.5 grains per 100 microns2, n = 10) but the level of non-specific binding of 123I-oPRL was higher than for 125I-hGH (18.9 +/- 1.4 vs 6.1 +/- 0.6 grains per 100 microns2, n = 10; P < 0.001). Binding assays of receptors in crude microsomal membranes extracted from ovine skin were used to ascertain binding capacity and specificity.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: New Zealand Pastoral Agriculture Research Institute, Ruakura Agricultural Research Centre, Hamilton.
authors.forename: V J
authors.initials: VJ
authors.lastname: Choy
authors.forename: A J
authors.initials: AJ
authors.lastname: Nixon
authors.forename: A J
authors.initials: AJ
authors.lastname: Pearson
mesh_terms: D000818:Animals; D001345:Autoradiography; D005260:Female; D013006:Growth Hormone / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D011869:Radioligand Assay; D011981:Receptors, Prolactin / Q000302:isolation & purification* / Q000378:metabolism; D012680:Sensitivity and Specificity; D012756:Sheep / Q000378:metabolism*; D012867:Skin / Q000737:chemistry*; D013545:Sweat Glands / Q000737:chemistry; D014935:Wool
chemical_list: D011981:Receptors, Prolactin; D013006:Growth Hormone