title: Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily.
abstract: ATP-binding cassette (ABC) transporters share significant sequence identity within their ATP-binding domains. Degenerate oligonucleotides based on highly conserved portions of the ATP-binding domain genes were used to clone portions of two members of the ABC gene superfamily from Saccharomyces cerevisiae DNA. These genes were designated MDL1 and MDL2 (for multidrug resistance-like). Each MDL gene is predicted to encode a single set of transmembrane domains and a single ATP-binding domain, thus the MDL gene products are 'half-molecule' ABC proteins. The two genes were mapped to precise regions on chromosomes XII and XVI and show a considerable similarity to the mammalian P-glycoprotein/multidrug resistance (MDR) and peptide transporter (TAP) genes. Preliminary analysis of null mutants constructed by gene replacement has indicated that the MDL genes are not essential for viability of yeast. The sequences have been deposited in the GenBank data library under Accession Numbers L16958 (Locus YSCBCSA) and L16959 (Locus YSCBCSB).
authors.affiliation: Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201.
authors.forename: M
authors.initials: M
authors.lastname: Dean
authors.forename: R
authors.initials: R
authors.lastname: Allikmets
authors.forename: B
authors.initials: B
authors.lastname: Gerrard
authors.forename: C
authors.initials: C
authors.lastname: Stewart
authors.forename: A
authors.initials: A
authors.lastname: Kistler
authors.forename: B
authors.initials: B
authors.lastname: Shafer
authors.forename: S
authors.initials: S
authors.lastname: Michaelis
authors.forename: J
authors.initials: J
authors.lastname: Strathern
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics*; D016681:Genome, Fungal; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; C490069:MDL1 protein, S cerevisiae; C490070:MDL2 protein, S cerevisiae; D008562:Membrane Glycoproteins; D029701:Saccharomyces cerevisiae Proteins