Antiplatelet effects of clausine-D isolated from Clausena excavata.
Clausine-D inhibited concentration-dependently the aggregation and release reaction of washed rabbit platelets induced by arachidonic acid and collagen, without affecting those induced by U46619, PAF and thrombin. The IC50 values of clausine-D on arachidonic acid- and collagen-induced platelet aggregation were calculated to be 9.0 +/- 1.1 and 58.9 +/- 0.9 microM, respectively. Thromboxane B2 and prostaglandin D2 formation in platelets caused by arachidonic acid were also suppressed. Clausine-D inhibited increased intracellular concentration of calcium in platelets caused by arachidonic acid and collagen, and also abolished the generation of inositol monophosphate caused by arachidonic acid, but not that by collagen, U46619, PAF and thrombin. In human citrated platelet-rich plasma, clausine-D inhibited the secondary phase, but not the primary phase, of aggregation induced by epinephrine and ADP. These results indicate that the antiplatelet effect of clausine-D is due to inhibition of the formation of thromboxane A2.
Wu
C C
CC
Pharmacological Institute, College of Medicine, National Taiwan University, Taipei.
Ko
F N
FN
Wu
T S
TS
Teng
C M
CM
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000470:Alkaloids / Q000302:isolation & purification*; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D002227:Carbazoles / Q000302:isolation & purification*; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis*; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification*; D015230:Prostaglandin D2 / Q000378:metabolism; D011817:Rabbits; D013929:Thromboxane B2 / Q000378:metabolism
D000470:Alkaloids; D002227:Carbazoles; D010716:Phosphatidylinositols; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; C076711:clausine D; D016718:Arachidonic Acid; D013929:Thromboxane B2; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003094:Collagen; D015230:Prostaglandin D2; D004837:Epinephrine