Presence of gelatinase A and metalloelastase type protease at the plasma membrane of human skin fibroblasts. Influence of cytokines and growth factors on cell-associated metalloendopeptidase levels.
Gelatinase A and elastase type proteinase (Homsy, et al., 1988) present at plasma membranes of human skin fibroblasts (HSF) were separated by anion exchange chromatography on a DEAE Tris acryl M column. Elastase type proteinase (HSFE1) was able to convert 72 kDa progelatinase A to a lower 66 kDa M.W. active enzyme. Several cytokines (IL-1 beta, IL4, IL6), interferon gamma (IFN gamma) and tumor growth factor beta (TGF-beta) were studied for their ability to modify the levels of those plasma membrane associated proteinases. Among these mediators, only IL-1 beta was found to enhance the amounts of HSF membrane-bound HSFE1 and Gelatinase A.
Beranger
J Y
JY
Laboratoire de biologie du tissu conjonctif, ER 74 CNRS, Faculté de Médecine, Université Paris XII, Créteil, France.
Godeau
G
G
Frances
C
C
Robert
L
L
Hornebeck
W
W
D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002478:Cells, Cultured; D002648:Child; D002852:Chromatography, Ion Exchange; D016207:Cytokines / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D018093:Gelatinases / Q000032:analysis*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000032:analysis*; D008970:Molecular Weight; D010196:Pancreatic Elastase / Q000032:analysis*; D012867:Skin / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D016207:Cytokines; D006133:Growth Substances; D007375:Interleukin-1; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D015847:Interleukin-4; D007371:Interferon-gamma; D010196:Pancreatic Elastase; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2