title: Human CREM gene: evolutionary conservation, chromosomal localization, and inducibility of the transcript.
abstract: The CREM (cyclic AMP-responsive element modulator) gene encodes multiple regulators of the cyclic AMP transcriptional response. CREM expression has been linked with several key physiological aspects of neuroendocrine pathways. We investigated the conservation of CREM during evolution. Here, we show conservation of CREM sequences in the pig, humans, the chicken, the lemur, and Xenopus. We have also determined the chromosomal localization of the CREM and CREB genes both in the mouse and in humans. We cloned the full human CREM complementary DNA sequence and demonstrate that it has a high degree of sequence identity with the mouse gene. Finally, we show the conservation of CREM cyclic AMP transcriptional inducibility in humans and establish that the induced transcripts correspond to the mouse ICER products.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de Médecine, Strasbourg, France.
authors.forename: D
authors.initials: D
authors.lastname: Masquilier
authors.forename: N S
authors.initials: NS
authors.lastname: Foulkes
authors.forename: M G
authors.initials: MG
authors.lastname: Mattei
authors.forename: P
authors.initials: P
authors.lastname: Sassone-Corsi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D017124:Conserved Sequence; D051786:Cyclic AMP Response Element Modulator; D017362:Cyclic AMP Response Element-Binding Protein / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D005809:Genes, Regulator; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics*; D017385:Sequence Homology; D015398:Signal Transduction / Q000235:genetics
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D051786:Cyclic AMP Response Element Modulator