A simplified rapid method for restriction fragment length polymorphism analysis after bone marrow transplantation.
A rapid and simple protocol for restriction fragment length polymorphism (RFLP) analysis using digoxigenin labeled DNA probes is presented. The method described gives consistently good results even with minute cell numbers. The method has been used to evaluate chimerism after bone marrow transplantation but could also find other applications.
Schreiner
T
T
Red Cross Blood Center, University of Ulm, Germany.
Wiesneth
M
M
Maccari
B
B
Sawodny
B
B
Prochnow-Calzia
H
H
Kubanek
B
B
D015139:Blotting, Southern; D001853:Bone Marrow / Q000473:pathology*; D016026:Bone Marrow Transplantation / Q000473:pathology*; D002678:Chimera / Q000235:genetics*; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008722:Methods; D012150:Polymorphism, Restriction Fragment Length; D014019:Tissue Donors
D015342:DNA Probes; D004247:DNA