Effects of the Anemonia sulcata toxin (ATX II) on intracellular sodium and contractility in rat and guinea-pig myocardium.
The effects of the Anemonia sulcata toxin ATX II on action potentials and contractility of isolated papillary muscles and single myocytes from rat and guinea-pig hearts have been studied. ATX II prolonged the action potential in both rat and guinea-pig papillary muscle. Although it produced a positive inotropic effect in guinea-pig papillary muscle, it failed to do so in rat papillary muscle. However, in single rat and guinea-pig ventricular cells, it both prolonged the action potential and had a positive inotropic effect. We suggest that ATX II does not cause a positive inotropic effect in rat papillary muscle, because it induces Ca2+ overload. In single cells the positive inotropic effect was reduced by approximately 50% when the contractions were triggered by voltage clamp pulses of constant duration rather than by action potentials. This suggests that the inotropic effect of ATX II is in part the result of the prolongation of the action potential. The intracellular Na+ activity (a(i)Na) in single ventricular cells was measured with the Na(+)-sensitive fluorescent dye SBFI. After exposure of the cells to ATX II, a(i)Na was increased by a maximum of 1.9 +/- 0.3 and 2.2 +/- 0.3 mM in rat and guinea-pig cells, respectively. It is suggested that the positive inotropic effect of ATX II is also in part the result of the rise in a(i)Na.
Hoey
A
A
Institute of Pharmacology, University of Essen, Germany.
Harrison
S M
SM
Boyett
M R
MR
Ravens
U
U
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002138:Calibration; D002316:Cardiotonic Agents / Q000633:toxicity*; D003064:Cnidarian Venoms / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects*; D009498:Neurotoxins / Q000008:administration & dosage / Q000633:toxicity*; D010210:Papillary Muscles / Q000187:drug effects; D018408:Patch-Clamp Techniques; D051381:Rats; D012615:Sea Anemones / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*
D002316:Cardiotonic Agents; D003064:Cnidarian Venoms; D009498:Neurotoxins; C028800:toxin II (Anemonia sulcata); D012964:Sodium; D002118:Calcium