The Myb and Ahi-1 genes are physically very closely linked on mouse chromosome 10.
Ahi-1 has previously been identified as a common helper provirus integration site on mouse Chromosome (Chr) 10 in 16% of Abelson pre-B-cell lymphomas and shown to be closely linked to the Myb protooncogene. By using long-range restriction mapping, we have mapped the Myb and Ahi-1 regions within a 120-kbp DNA fragment. The Ahi-1 region is located approximately 35 kbp downstream of the Myb gene. A further confirmation of this finding was obtained by screening a mouse YAC library. The three positive clones obtained contained both the Myb and Ahi-1 gene sequences. To test whether provirus integration in the Ahi-1 region enhances the expression of Myb by a cis-acting mechanism, we have also examined Myb gene expression in A-MuLV-induced pre-B-lymphomas. Our data have revealed that there is no clear evidence for such activation in the tumors we have tested, indicating that provirus insertion in the Ahi-1 region is activating a novel gene, apparently involved in tumor formation.
Jiang
X
X
Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Quebec, Canada.
Villeneuve
L
L
Turmel
C
C
Kozak
C A
CA
Jolicoeur
P
P
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA; D016521:Electrophoresis, Gel, Pulsed-Field; D015972:Gene Expression Regulation, Neoplastic; D008040:Genetic Linkage; D006378:Helper Viruses / Q000235:genetics; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020598:Proto-Oncogene Proteins c-myb; D011533:Proviruses / Q000235:genetics; D015183:Restriction Mapping; D016662:Virus Integration / Q000235:genetics
D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D004247:DNA