In vitro intercellular adhesion molecule-1 expression on brain endothelial cells in multiple sclerosis.
To investigate the origin of intercellular adhesion molecule-1 (ICAM-1) and its expression on brain endothelial cells, we studied the expression in vitro of ICAM-1 on human brain endothelial cells after incubation of T cells from patients with multiple sclerosis (MS) using a histochemical technique and flow cytometry. We determined soluble forms of ICAM-1 (ICAM-1) in the supernatants after mixtures of brain endothelial cells and T cells from patients with MS using an enzyme-liked immunosorbent assay. Flow cytometric analysis showed that a number of ICAM-1-positive cells were significantly increased after incubation of brain endothelial cells with T cells from patients with acute relapsing MS during an exacerbation as compared with those of controls (P < 0.01). Patients with acute relapsing MS during an exacerbation and chronic progressive MS exhibited higher levels of ICAM-1 in the supernatants of mixtures with brain endothelial cells and lymphocytes than those of controls (P < 0.001 and P < 0.01, respectively). These results suggest that lymphocytes from patients with acute relapsing MS during an exacerbation lead to an increased expression of ICAM-1 on the brain endothelial cells and add to evidence involving this adhesion molecule in the pathogenesis of MS.
Tsukada
N
N
Department of Health Medical Center and Neurology, Shinshu University, Matsumoto, Japan.
Matsuda
M
M
Miyagi
K
K
Yanagisawa
N
N
D000328:Adult; D001923:Brain Chemistry; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000737:chemistry*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1