Expression of proliferation-associated proteins (proliferating cell nuclear antigen and Ki-67 antigen) in Bowen's disease.
Bowen's disease and invasive squamous cell carcinoma arising in Bowen's disease (Bowen's carcinoma) were investigated immunohistochemically with regard to their expression of proliferating cell nuclear antigen (PCNA) and the Ki-67 antigen. Both proliferation-associated proteins were found in all layers of the tumours, and were present in nuclei of any size and shape. The nucleoli lacked PCNA immunoreactivity, but they expressed the Ki-67 antigen. The PCNA labelling index was 36.9 +/- 12.6% in Bowen's disease (n = 16), and 40.7 +/- 11.0% in Bowen's carcinoma (n = 3). The Ki-67 antigen labelling index was 43.6 +/- 16.7% in Bowen's disease (n = 16), and 51.7 +/- 11.6% in Bowen's carcinoma (n = 3). Dyskeratotic cells were positive for PCNA and the Ki-67 antigen, suggesting that these cells are not in a post-mitotic state. We conclude that in dyskeratotic cells of Bowen's disease the cell cycle is interrupted by nuclear disorganization.
Baum
H P
HP
Department of Dermatology, University of Saarland, Homburg/Saar, Germany.
Meurer
I
I
Unteregger
G
G
D000951:Antigens, Neoplasm / Q000032:analysis*; D001913:Bowen's Disease / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D002467:Cell Nucleus / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D012044:Regression Analysis; D012878:Skin Neoplasms / Q000276:immunology*
D000951:Antigens, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen