Differential-scanning-calorimetric study of the irreversible thermal denaturation of 8 kDa cytotoxin from the sea anemone Radianthus macrodactylus.
A differential-scanning-calorimetric study of the thermal denaturation of a sea-anemone (Radianthus macrodactylus) 8 kDa cytolytic toxin was carried out. The calorimetric traces were found to be irreversible and scan-rate-dependent under the experimental conditions employed. Scan-rate-dependent thermograms were explained in terms of a two-state kinetic model N k -->D, where k is a first-order kinetic constant that changes with temperature as given by the Arrhenius equation, N is the native state of the toxin, and D the denatured one.
Zhadan
G G
GG
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, Spain.
Shnyrov
V L
VL
D000818:Animals; D002152:Calorimetry, Differential Scanning; D003064:Cnidarian Venoms / Q000737:chemistry*; D003603:Cytotoxins / Q000737:chemistry*; D006358:Hot Temperature; D007700:Kinetics; D011489:Protein Denaturation; D012615:Sea Anemones / Q000737:chemistry*; D013816:Thermodynamics
D003064:Cnidarian Venoms; D003603:Cytotoxins