Intra-erythrocytic Plasmodium falciparum induces up-regulation of inter-cellular adhesion molecule-1 on human endothelial cells in vitro.
The stimulation of human vascular endothelial cells with P. falciparum-infected erythrocytes resulted in the non-transient up-regulation of ICAM-1 expression on human endothelial cells. The induction was independent from TNF as assured in various controls including the application of a TNF-neutralizing antibody. The possibility that TNF is produced by endothelial cells was investigated in a purified culture of human endothelial cells by TNF-ELISA and TNF-bio-assay both with negative results. This result may be evidence of the existence of TNF-independent mechanisms in the pathogenesis of human cerebral malaria.
Esslinger
C W
CW
Department of Parasitology-Mycology Medical and Molecular (DP3M) Faculty of Medicine, CNRS ERS 15, Grenoble, France.
Picot
S
S
Ambroise-Thomas
P
P
D000818:Animals; D015815:Cell Adhesion Molecules / Q000502:physiology*; D004730:Endothelium, Vascular / Q000737:chemistry / Q000166:cytology*; D004912:Erythrocytes / Q000469:parasitology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D008264:Macrophages / Q000378:metabolism; D010963:Plasmodium falciparum / Q000302:isolation & purification*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology; D015854:Up-Regulation
D015815:Cell Adhesion Molecules; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1