Characterisation of Staphylococcus intermedius isolates from canine pyoderma and from healthy carriers by SDS-PAGE of exoproteins, immunoblotting and restriction endonuclease digest analysis.
Ten Staphylococcus intermedius isolates from cases of canine pyoderma and 10 from healthy carriers were examined by SDS-PAGE of exoproteins, immunoblotting and restriction endonuclease digest analysis. Similarities between banding patterns of the isolates were calculated as Dice coefficients for all three methods. For SDS-PAGE and immunoblotting, no significant differentiation was found between the pyoderma and "healthy" groups. Analysis of DNA digested with BglII indicated that S. intermedius is genetically heterogeneous; Dice coefficients for the pyoderma group were distinct from those for the healthy group (p < 0.001), and cluster analysis confirmed that the pyoderma isolates (9) formed a group separate from the majority (6 of 9) of the normal isolates.
Allaker
R P
RP
Dermatology Unit, Royal Veterinary College, North Mimms, Hertfordshire.
Garrett
N
N
Kent
L
L
Noble
W C
WC
Lloyd
D H
DH
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000032:analysis; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D012150:Polymorphism, Restriction Fragment Length; D011711:Pyoderma / Q000382:microbiology / Q000662:veterinary*; D013207:Staphylococcal Skin Infections / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000737:chemistry / Q000145:classification* / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial