Soluble intercellular adhesion molecule-1 in arthritis.
The objective of this study is to determine whether soluble intercellular adhesion molecule-1 (sICAM-1) is found in rheumatoid arthritis (RA) and other inflammatory disorders and to identify which cells are responsible for sICAM-1 production. Synovial fluid, blood and cells isolated from RA synovial fluids, and synovial tissues from 59 patients were studied. In addition, normal peripheral blood was obtained. sICAM-1 was assayed by an enzyme-linked immunoabsorbent assay. Synovial fluids from patients with RA and other inflammatory arthritides had significantly higher sICAM-1 levels than did osteoarthritis (OA) synovial fluids. Synovial fluid sICAM-1 levels were significantly positively correlated with synovial fluid leukocyte counts. RA synovial tissue fibroblasts released low levels of sICAM-1. Neutrophils (PMNs) isolated from synovial fluids of RA patients spontaneously released sICAM-1. However, mononuclear cells isolated from RA synovial fluid produced the largest quantities of sICAM-1. Phytohemagglutinin but not lipopolysaccharide enhanced mononuclear sICAM-1 release. sICAM-1 was increased in synovial fluids from RA compared to OA. This sICAM-1 may be important in modulating the trafficking of inflammatory leukocytes into diseased RA synovial tissue and fluid.
Koch
A E
AE
Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611.
Shah
M R
MR
Harlow
L A
LA
Lovis
R M
RM
Pope
R M
RM
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood / Q000378:metabolism*; D012016:Reference Values; D012995:Solubility; D013582:Synovial Fluid / Q000737:chemistry / Q000166:cytology / Q000378:metabolism
D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1