Genotyping and association analysis of HLA-B61 in Japanese.
The distribution of HLA-B61-related alleles, B*4002-B*4006, was examined in the Japanese population by using PCR-SSO and PCR-RFLP methods. About half of the B61-positive individuals possessed B*4002 and the remaining half possessed B*4006. In addition, these two major B61 alleles were separately associated with different HLA-C alleles: B*4002 exhibited a strong linkage disequilibrium with Cw10, whereas B*4006 was strongly associated with C blank and DR9. Amino acid residues that contribute to the serologic epitopes of the B61 group and their relationships with other HLA-B locus antigens are discussed.
Lin
L
L
Department of Transfusion Medicine and Immunohematology Faculty of Medicine, University of Tokyo, Japan.
Tokunaga
K
K
Ogawa
A
A
Ishikawa
Y
Y
Kashiwase
K
K
Akaza
T
T
Kuwata
S
S
Tadokoro
K
K
Juji
T
T
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D016384:Consensus Sequence; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017386:Sequence Homology, Amino Acid
D015235:HLA-B Antigens; C079077:HLA-B61 antigen; D015345:Oligonucleotide Probes; D004247:DNA