Immunocytochemical localization of AMPA selective glutamate receptor subunits in the rat cochlea.
The localization of subunits of the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) selective glutamate receptor, termed Glutamate receptor (GluR) was examined in the rat cochlea using affinity purified polyclonal antibody to GluR subunits (GluR 1-4). GluR 2/3 and GluR 4 immunoreactive (IR) staining was observed in rat spiral ganglion cells, while GluR 1 IR was not. GluR 4 IR staining was also seen in puncta at inner and outer hair cell bases. These results suggest that GluR 2/3 and GluR 4 are components of excitatory amino acid synapses in the rat cochlea.
Kuriyama
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H
Kresge Hearing Research Institute, Department of Otolaryngology, University of Michigan, Ann Arbor 48109-0506.
Jenkins
O
O
Altschuler
R A
RA
D000818:Animals; D015153:Blotting, Western; D003051:Cochlea / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005629:Frozen Sections; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism; D007150:Immunohistochemistry; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000032:analysis* / Q000187:drug effects / Q000276:immunology
D018091:Receptors, AMPA