Thick bone section preparation using a silicon-rubber-based sealant.
A method has been developed, using a silicon-rubber-based sealant, which allows 2-3-mm-thick specimens to be maintained in a protected fluid environment for a number of months, without risk of dehydration. Following this, the specimen can be retrieved, stained, embedded and sectioned further. For example, 2-mm-thick sections of fixed unstained bone are easily examined by means of epi-illuminated polarized light and fluorescence microscopies using either conventional or confocal optics. The method could easily be extended to other tissues, for example brain tissue.
Cox
H
H
Department of Orthopaedic and Accident Surgery, University of Liverpool, U.K.
Walker
C
C
Howard
C V
CV
D001842:Bone and Bones; D012826:Silicone Elastomers; D016707:Tissue Fixation / Q000379:methods*
D012826:Silicone Elastomers