Restriction fragment length polymorphism (RFLP) of genomic DNA of Moraxella (Branhamella) catarrhalis isolates in a hospital.
Epidemiological typing, based on restriction fragment length polymorphism (RFLP) by pulsed-field gel electrophoresis (PFGE), was attempted for the 38 clinical isolates of Moraxella catarrhalis obtained at Shinshu University Hospital during the years 1987 and 1993. Digestion with SmaI or NotI generated well separable, 12 to 5 genomic DNA fragments ranging from 1,000 kb to 30 kb and the strains could be classified into 14 or 13 types, respectively. The electrophoretic profile differed with the strain in most of them and was hence useful to distinguish the each strain. Investigation for their RFLP have, however, suggested that majority of them, including the type strain ATCC25238, may have derived from a common ancestor.
Kawakami
Y
Y
Central Clinical Laboratories, Shinshu University Hospital, Nagano, Japan.
Ueno
I
I
Katsuyama
T
T
Furihata
K
K
Matsumoto
H
H
D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D001936:Moraxella catarrhalis / Q000145:classification*; D016870:Neisseriaceae Infections / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length