title: Roller and centrifugal pumps compared in vitro with regard to haemolysis, granulocyte and complement activation.
abstract: A Biomedicus centrifugal pump and a Polystan roller pump were compared in vitro with regard to differences in haemolysis, granulocyte and complement activation. Six circuits of tubing and oxygenators were connected to each pump. Heparinized fresh human blood was circulated for 72 hours in the systems. Blood samples were drawn at defined intervals. Haemolysis was assessed by determination of lactate dehydrogenase (LD) and potassium, and granulocyte activation by quantification of the granulocyte proteins calprotectin, lactoferrin and myeloperoxidase. Complement activation was assessed by measuring C3 activation products (C3b, iC3b and C3c), and the terminal C5b-9 complement complex (TCC). The results indicate more haemolysis and complement activation in the roller pump group, revealed by significantly higher concentrations of LD, potassium, C3 activation products and TCC. Calprotectin, lactoferrin and myeloperoxidase were all significantly increased in both groups, but the rise appeared earlier in the roller pump group. The concentrations of LD and potassium both correlated significantly with C3 activation products, indicating that complement activation may at least partly be responsible for the haemolysis.
authors.affiliation: Department of Cardiothoracic Surgery, Ullevål Hospital, Oslo, Norway.
authors.forename: O
authors.initials: O
authors.lastname: Moen
authors.forename: E
authors.initials: E
authors.lastname: Fosse
authors.forename: J
authors.initials: J
authors.lastname: Bråten
authors.forename: C
authors.initials: C
authors.lastname: Andersson
authors.forename: M K
authors.initials: MK
authors.lastname: Fagerhol
authors.forename: P
authors.initials: P
authors.lastname: Venge
authors.forename: K
authors.initials: K
authors.lastname: Høgåsen
authors.forename: T E
authors.initials: TE
authors.lastname: Mollnes
mesh_terms: D015816:Cell Adhesion Molecules, Neuronal / Q000097:blood; D003167:Complement Activation; D003176:Complement C3 / Q000032:analysis; D015938:Complement Membrane Attack Complex / Q000032:analysis; D004867:Equipment Design; D006098:Granulocytes / Q000502:physiology*; D006353:Heart-Assist Devices / Q000009:adverse effects; D006461:Hemolysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007781:Lactoferrin / Q000097:blood; D039841:Leukocyte L1 Antigen Complex; D009195:Peroxidase / Q000097:blood; D011188:Potassium / Q000097:blood
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D003176:Complement C3; D015938:Complement Membrane Attack Complex; D039841:Leukocyte L1 Antigen Complex; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase; D007781:Lactoferrin; D011188:Potassium