title: A new method for direct analysis of polymerase chain reaction-amplified human papillomavirus using DNA enzyme immunoassay.
abstract: We describe use of a polymerase chain reaction in conjunction with a DNA enzyme immunoassay for the simultaneous detection of multiple types of human papillomavirus in a single microtiter plate. The method is specific, sensitive, rapid, and capable of detecting human papillomavirus-6, -11, -16, -18, and -33 in cervical biopsy specimens.
authors.affiliation: Department of Human Genetics, Tor Vergata University of Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Sangiuolo
authors.forename: L
authors.initials: L
authors.lastname: De Santis
authors.forename: A
authors.initials: A
authors.lastname: Cavicchini
authors.forename: U
authors.initials: U
authors.lastname: Angeloni
authors.forename: C
authors.initials: C
authors.lastname: Romanini
authors.forename: G
authors.initials: G
authors.lastname: Novelli
authors.forename: B
authors.initials: B
authors.lastname: Dallapiccola
mesh_terms: D001483:Base Sequence; D015344:DNA Probes, HPV; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D015344:DNA Probes, HPV