Establishment and characterization of an HTLV-I cell line from a Taiwanese patient with HTLV-I-associated myelopathy.
We describe a Taiwanese woman with chronic progressive myelopathy, in whom Western blot analysis of the serum and cerebrospinal fluid (CSF) displayed positive reactions to human T-lymphotropic virus type I (HTLV-I) proteins, p19, p24, p28, p36, gp46 and p53. HTLV-I proviral genomes were detected in the peripheral blood mononuclear cells (PBMC) and CSF cells by nested polymerase chain reaction and Southern blot hybridization. HTLV-I was successfully isolated from PBMC stimulated with interleukin-2 (IL-2). The established cell line, named THAM-1, was an IL-2-independent T-cell line with CD2+, CD3+, CD4+, CD25+ and HLA-DR+. Retrovirus particles with type C morphology were observed in the THAM-1 cells by electron microscopy, and HTLV-I-related antigens were also demonstrated by immunocytochemical staining and Western blot assay. Southern blot analysis revealed that HTLV-I proviral genomes were integrated into the THAM-1 cellular DNA. In Northern blot analysis, two extra-species of RNA were detected in addition to three typical viral transcripts. For the first time, an HTLV-I-producing T cell line was established from a patient with HTLV-I-associated myelopathy in Taiwan, an HTLV-I non-endemic area.
Yang
Y C
YC
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei.
Hung
T P
TP
Wang
C H
CH
Lin
M T
MT
Hsu
T Y
TY
Chen
J Y
JY
Chen
Y C
YC
Yang
C S
CS
D000328:Adult; D015153:Blotting, Western; D002353:Carrier State; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D015485:Deltaretrovirus Antigens / Q000276:immunology; D005260:Female; D016679:Genome, Viral; D015481:HTLV-I Antibodies / Q000097:blood / Q000134:cerebrospinal fluid; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D015493:Paraparesis, Tropical Spastic / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*; D013601:T-Lymphocytes / Q000382:microbiology* / Q000473:pathology / Q000648:ultrastructure; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure; D016662:Virus Integration
D015485:Deltaretrovirus Antigens; D015481:HTLV-I Antibodies