title: Effect of low temperatures on the transfer of phospholipids with various acyl-chain lengths to the plasma membrane of leek cells.
abstract: The intracellular transport of lipids with very-long-chain acyl moieties (C20-C24 fatty acid-containing lipids) to the plasma membrane of leek cells follows the vesicular ER-Golgi apparatus-plasma membrane pathway. Here we report on the effect of low temperatures on the vesicular transport of lipids and especially C20-C24 fatty acid-containing lipids to the plasma membrane of leek cells. These lipids, normally transported through the ER-Golgi apparatus-plasma membrane pathway at 24 degrees C, accumulated in the ER and the Golgi apparatus at 12 degrees C with a related deficit in the plasma membrane. As lipids with long chain acyl moieties (C16 and C18 fatty acid-containing lipids) were still transferred to the plasma membrane at 12 degrees C, the data demonstrate a specific effect of low temperatures on the vesicular transfer of C20-C24 fatty acid-containing lipids to the plasma membrane of leek cells. Therefore, evidence is provided for distinct pathways and/or mechanisms transferring lipids to the plasma membrane of leek cells which differ in their sensitivity to low temperatures.
authors.affiliation: URA 1811 CNRS, IBGC, University of Bordeaux II, France.
authors.forename: P
authors.initials: P
authors.lastname: Moreau
authors.forename: B
authors.initials: B
authors.lastname: Sturbois
authors.forename: D J
authors.initials: DJ
authors.lastname: Morré
authors.forename: C
authors.initials: C
authors.lastname: Cassagne
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D003080:Cold Temperature; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D006056:Golgi Apparatus / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D010944:Plants / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids