Use of random fragments of chromosomal DNA to highlight restriction site heterogeneity for fingerprinting isolates of Salmonella typhimurium from hospitalized animals.
Random fragments of DNA were obtained from a cosmid library of Salmonella agona genomic DNA. From this library, 2 fragments were chosen and pooled to probe isolates of S typhimurium obtained during an episode of salmonellosis in a veterinary medical teaching hospital. Chromosomal DNA from the Salmonella isolates was digested with restriction endonucleases, and was probed with the random fragments of chromosomal DNA. This procedure resulted in a fingerprint pattern for each isolate. We found that the method permitted discrimination between isolates involved in the disease episode and S typhimurium obtained prior to the episode. We conclude that random fragments of chromosomal DNA are useful for fingerprinting isolates of S typhimurium. Analysis of plasmid DNA obtained from the isolates was not as useful. Some isolates found to be identical by restriction site analysis, had plasmids of different molecular weight. These results indicate that plasmid analysis may not be as useful a fingerprinting tool as previously reported.
Hansen
L M
LM
Department of Veterinary Microbiology and Immunology, Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis 95616.
Jang
S S
SS
Hirsh
D C
DC
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D003360:Cosmids; D016172:DNA Fingerprinting / Q000662:veterinary*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004372:Ducks; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses; D006762:Hospitals, Animal; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000302:isolation & purification
D015342:DNA Probes; D004269:DNA, Bacterial