title: Application of fluorescence in situ hybridization in defining a complex t(9;21;22) Ph formation.
abstract: We describe the application of fluorescence in situ hybridization (FISH) in a case of suspected chronic myelogenous leukemia (CML), cytogenetically characterized by a t(21;22) with no clear involvement of chromosome 9. The dual color FISH technique, performed using specific painting probes for chromosomes 9,21,22 and a BCR/ABL translocation probe, enabled us to confirm the diagnosis of CML by detecting the BCR/ABL rearrangement on chromosome 22q and the involvement of chromosome 9 in a variant translocation t(9;21;22).
authors.affiliation: Department of Human Biopathology, University La Sapienza, Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mancini
authors.forename: M
authors.initials: M
authors.lastname: Nanni
authors.forename: M
authors.initials: M
authors.lastname: Cedrone
authors.forename: M R
authors.initials: MR
authors.lastname: De Cuia
authors.forename: M B
authors.initials: MB
authors.lastname: Rondinelli
authors.forename: F
authors.initials: F
authors.lastname: Malagnino
authors.forename: G
authors.initials: G
authors.lastname: Alimena
mesh_terms: D000328:Adult; D002891:Chromosomes, Human, Pair 21 / Q000648:ultrastructure*; D002892:Chromosomes, Human, Pair 22 / Q000648:ultrastructure*; D002899:Chromosomes, Human, Pair 9 / Q000648:ultrastructure*; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D008297:Male; D010677:Philadelphia Chromosome; D014178:Translocation, Genetic
chemical_list: D016044:Fusion Proteins, bcr-abl