Aspartate aminotransferase and glutaminase activities in rat olfactory bulb and cochlear nucleus; comparisons with retina and with concentrations of substrate and product amino acids.
The quantitative distributions of aspartate aminotransferase and glutaminase were mapped in subregions of olfactory bulb and cochlear nucleus of rat, and were compared with similar data for retina and with the distributions of their substrate and product amino acids aspartate, glutamate, and glutamine. The distributions of both enzymes paralleled that of aspartate in the olfactory bulb and that of glutamate in the cochlear nucleus. In retina (excluding inner segments), there were similarities between aspartate aminotransferase and both glutamate and aspartate distributions. The distribution of gamma-aminobutyrate (GABA) was similar to those of both enzymes in olfactory bulb, to aspartate aminotransferase in cochlear nucleus, and to glutaminase in retina (excluding inner segments). The results are consistent with significant involvement of aspartate aminotransferase, especially the cytosolic isoenzyme, and glutaminase in accumulation of the neurotransmitter amino acids glutamate, aspartate, and GABA, although with preferential accumulation of different amino acids in different brain regions.
Godfrey
D A
DA
Department of Otolaryngology-Head and Neck Surgery, Medical College of Ohio, Toledo 43699-0008.
Ross
C D
CD
Parli
J A
JA
Carlson
L
L
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism; D017626:Cochlear Nucleus / Q000033:anatomy & histology / Q000201:enzymology*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D005972:Glutaminase / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D009830:Olfactory Bulb / Q000033:anatomy & histology / Q000201:enzymology*; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000033:anatomy & histology / Q000201:enzymology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000596:Amino Acids; D005971:Glutamates; D007527:Isoenzymes; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D008291:Malate Dehydrogenase; D001219:Aspartate Aminotransferases; D005972:Glutaminase