A small chromosome of Leishmania (Viannia) braziliensis contains multicopy sequences which are complex specific.
Orthogonal Field Alternating Gel Electrophoresis (OFAGE) has been used to show a band of approximately 260 kb which is stained intensely with ethidium bromide in Leishmania (V.) braziliensis stock M2903. This small chromosome (sc-2903), as well as a 50 kb and a 200 kb chromosome seen in L. (L.) mexicana and L. (L.) amazonensis, respectively, are stably maintained and linear. When used as a hybridisation probe, sc-2903 showed homology to large chromosomal DNA bands and to a multiplicity of genomic fragments in all braziliensis stocks tested, indicating either different sequences, different copy numbers or both but no hybridisation to mexicana stocks. It is possible that these sequences are present in all members of the braziliensis complex and are not related to LD1 or any other previously published small chromosome sequences. However, at least one clone isolated from a sc-2903 library recognised genomic DNA of stocks belonging to the braziliensis, mexicana and donovani complexes. Our results suggest that the clone carries sequence(s) that are repeated and shared between stocks of different complexes but with a variable genomic distribution.
Eresh
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MRC Outstation of NIMR, Molteno Laboratories, Department of Pathology, University of Cambridge, UK.
Mendoza-Leòn
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Barker
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D000818:Animals; D015139:Blotting, Southern; D002875:Chromosomes; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D006801:Humans; D007621:Karyotyping; D007892:Leishmania braziliensis / Q000145:classification / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D016054:DNA, Protozoan