Ontogeny of small intestinal glutaminase and glutamine synthetase in the rat: response to dexamethasone.
The ontogeny of small intestinal glutaminase (GA) and glutamine synthetase (GS) was studied with relation to the maternal administration of dexamethasone (DEX). Pregnant Sprague-Dawley rats were administered 0.2 mg/kg of DEX or saline twice daily on days 19 and 20 of gestation. Activity of GA did not increase from the fetus to the 10-day-old rat; however, GS activity increased 7-fold. DEX induced doubling of GA activity and mRNA, but only a slight increase is GS activity and mRNA was observed in the fetus. GA and GS activities appear to be regulated by different mechanisms.
Meetze
W H
WH
Department of Pediatrics, University of Florida, Gainesville 32610.
Shenoy
V
V
Martin
G
G
Musy
P
P
Neu
J
J
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D000833:Animals, Suckling / Q000378:metabolism; D015152:Blotting, Northern; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D005333:Fetus / Q000201:enzymology*; D005865:Gestational Age; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism*; D005972:Glutaminase / Q000235:genetics / Q000378:metabolism*; D007421:Intestine, Small / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D003907:Dexamethasone; D005972:Glutaminase; D005974:Glutamate-Ammonia Ligase