title: Mast cells are one major source of interleukin-4 in atopic dermatitis.
abstract: Several reports have shown the presence of T-helper lymphocytes with Th2 characteristics in the skin lesions of atopic dermatitis (AD). However, Th2 cells themselves require an exogenous pulse of IL-4 to initiate their differentiation and synthesis of IL-4. As mast cells have recently been shown to contain IL-4, this finding prompted us to investigate IL-4 in mast cells of AD lesions, to determine if they might provide the IL-4 pulse needed by the Th2 cells. In this study, we measured IL-4 immunoreactivity in mast cells of non-lesional and lesional skin sections from 20 patients with AD. Ten patients with nummular eczema (NE) without any atopic features or background, and five healthy subjects, served as the control groups. Mast cells were first identified using an enzyme--histochemical staining method for tryptase. Subsequently, the sections were photographed, the tryptase stain was removed, and IL-4 was demonstrated with a polyclonal antibody. The sections were photographed again, and the percentage of IL-4 positive mast cells was calculated. The percentage of mast cells exhibiting IL-4 immunoreactivity in the upper dermis in lesional vs. non-lesional skin was 66 +/- 18% vs. 37 +/- 18% in AD (P < 0.0001, paired t-test), but only 46 +/- 19% vs. 31 +/- 22% in NE. In the skin of healthy controls, only 23 +/- 25% of the mast cells were positive for IL-4. In addition, a significant difference was found between lesional skin of AD vs. NE patients (P < 0.008, unpaired t-test).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Dermatology, Kuopio University Hospital, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Horsmanheimo
authors.forename: I T
authors.initials: IT
authors.lastname: Harvima
authors.forename: A
authors.initials: A
authors.lastname: Järvikallio
authors.forename: R J
authors.initials: RJ
authors.lastname: Harvima
authors.forename: A
authors.initials: A
authors.lastname: Naukkarinen
authors.forename: M
authors.initials: M
authors.lastname: Horsmanheimo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000276:immunology*; D004485:Eczema / Q000276:immunology; D006801:Humans; D015847:Interleukin-4 / Q000096:biosynthesis*; D008407:Mast Cells / Q000378:metabolism*; D008875:Middle Aged
chemical_list: D015847:Interleukin-4