Isolation of an insertion sequence (IS1051) from Xanthomonas campestris pv. dieffenbachiae with potential use for strain identification and characterization.
A new insertion sequence was isolated from Xanthomonas campestris pv. dieffenbachiae. Sequence analysis showed that this element is 1,158 bp long and has 15-bp inverted repeat ends containing two mismatches. Comparison of this sequence with sequences in data bases revealed significant homology with Escherichia coli IS5. IS1051, which detected multiple restriction fragment length polymorphisms, was used as a probe to characterize strains from the pathovar dieffenbachiae.
Berthier
Y
Y
Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, France.
Thierry
D
D
Lemattre
M
M
Guesdon
J L
JL
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010944:Plants / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D011548:Pseudomonadaceae / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D016959:Xanthomonas campestris / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial