Robotic sample preparation and high-performance liquid chromatographic analysis of verlukast in human plasma.
A fully automated HPLC assay has been developed and validated for the quantitation of verlukast, a leukotriene D4 antagonist, in human plasma. An upgraded Zymate I robotic system was utilized to perform protein precipitation and on-line injection followed by reversed-phase HPLC with fluorescence detection. Inter-day accuracy and precision were 100.8 and 4.6%, respectively, for the low quality control standards (0.125 microgram/ml). The automated robotic method was shown to be more efficient and accurate than the manual method.
Hsieh
J Y
JY
Department of Drug Metabolism, Merck Research Laboratories, West Point, PA 19486.
Lin
C
C
Matuszewski
B K
BK
D001993:Bronchodilator Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D011422:Propionates / Q000097:blood*; D011786:Quality Control; D011804:Quinolines / Q000097:blood*; D015203:Reproducibility of Results; D012371:Robotics; D013048:Specimen Handling; D013050:Spectrometry, Fluorescence
D001993:Bronchodilator Agents; D011422:Propionates; D011804:Quinolines; C059141:verlukast