[Establishment of the multidrug-resistant cell line K562/A02 and its drug-resistant properties].
A multidrug-resistant cell line K562/A02 was obtained from its parent K562 by long-term adriamycin (ADM) induction and cloning selection. The K562/A02 has high expression of mdr-1 P-glycoprotein (P170), strong resistance to its induction drug ADM and some other anticancer drugs such as daunorubicin, VP16, homoharringtonine and amsacrine. Its intracellular concentration of daunorubicin is much lower than that in its parent K562, which can be partially reversed by cyclosporin-A and verapamil.
Luan
F J
FJ
Institute of Hematology, CAMS, Tianjin.
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002352:Carrier Proteins / Q000032:analysis; D003630:Daunorubicin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D004351:Drug Resistance; D006248:Harringtonines / Q000494:pharmacology; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000187:drug effects
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D002352:Carrier Proteins; D006248:Harringtonines; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D004317:Doxorubicin; D003630:Daunorubicin