title: Mesodermal patterning by a gradient of the vertebrate homeobox gene goosecoid.
abstract: Amphibian mesoderm arises from the marginal zone of the early gastrula and generates various tissues such as notochord, muscle, kidney, and blood. Small changes (twofold) in the amount of microinjected messenger RNA encoding the goosecoid (gsc) homeodomain protein resulted in marked changes in the differentiation of mesoderm in Xenopus laevis. At least three thresholds were observed, which were sufficient to specify four mesodermal cell states. Endogenous gsc messenger RNA was expressed in the marginal zone in a graded fashion that is congruent with a role for this gene in dorso-ventral patterning of mesoderm at the early gastrula stage.
authors.affiliation: Molecular Biology Institute, University of California, Los Angeles 90024-1737.
authors.forename: C
authors.initials: C
authors.lastname: Niehrs
authors.forename: H
authors.initials: H
authors.lastname: Steinbeisser
authors.forename: E M
authors.initials: EM
authors.lastname: De Robertis
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D046508:Culture Techniques; D004268:DNA-Binding Proteins / Q000235:genetics*; D005775:Gastrula / Q000166:cytology* / Q000378:metabolism; D015870:Gene Expression; D005801:Genes, Homeobox; D051956:Goosecoid Protein; D018398:Homeodomain Proteins; D008648:Mesoderm / Q000166:cytology*; D008845:Microinjections; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins; D014157:Transcription Factors; D014982:Xenopus laevis
chemical_list: D004268:DNA-Binding Proteins; D051956:Goosecoid Protein; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors