title: Nine polymorphic markers characterized by polymerase chain reaction techniques form two linkage groups on rat chromosome 8.
abstract: Five genes and four anonymous polymorphic markers, forming two linkage groups, were mapped in F2 intercross progeny of F344/N x LEW/N rats using polymerase chain reaction (PCR) techniques. Both linkage groups were assigned to rat chromosome 8 because they contained genetic loci previously mapped to this chromosome. The first group was comprised of markers for three anonymous loci and two gene loci, thymus cell antigen-1 (Thy1) and tropoelastin (Eln). The second group was comprised of markers for one anonymous locus and three gene loci, cellular retinol binding protein II (Rbp2), matrin F/G (Matr1), and acyl-peptide hydrolase (Apeh). Seven markers (identified by simple sequence repeat associated length polymorphisms) were characterized in an additional 13 inbred rat strains (ACI/N, BN/SsN, BUF/N, LER/N, LOU/MN, MNR/N, MR/N, SHR/N, SR/Jr, SS/Jr, WBB1/N, WBB2/N, and WKY/N). Two to six alleles were detected for each marker. The reported markers should facilitate genetic mapping and monitoring of inbred rat strains.
authors.affiliation: Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: P
authors.initials: P
authors.lastname: Mathern
authors.forename: E A
authors.initials: EA
authors.lastname: Goldmuntz
authors.forename: Y
authors.initials: Y
authors.lastname: Du
authors.forename: H
authors.initials: H
authors.lastname: Zha
authors.forename: J M
authors.initials: JM
authors.lastname: Cash
authors.forename: L J
authors.initials: LJ
authors.lastname: Crofford
authors.forename: R L
authors.initials: RL
authors.lastname: Wilder
authors.forename: E F
authors.initials: EF
authors.lastname: Remmers
mesh_terms: D000483:Alleles; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017931:DNA Primers; D008040:Genetic Linkage; D005819:Genetic Markers; D020783:Matrix Metalloproteinase 7; D008562:Membrane Glycoproteins / Q000235:genetics; D008666:Metalloendopeptidases / Q000235:genetics; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012177:Retinol-Binding Proteins / Q000235:genetics; D054840:Retinol-Binding Proteins, Cellular; D013045:Species Specificity; D018800:Thy-1 Antigens; D014333:Tropoelastin / Q000235:genetics
chemical_list: D000954:Antigens, Surface; D017931:DNA Primers; D005819:Genetic Markers; D008562:Membrane Glycoproteins; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D018800:Thy-1 Antigens; D014333:Tropoelastin; D010447:Peptide Hydrolases; D008666:Metalloendopeptidases; D020783:Matrix Metalloproteinase 7