Proliferating cell nuclear antigen (PCNA) expression in regenerating rat liver after partial hepatectomy.
Proliferating cell nuclear antigen (PCNA) is a nuclear protein maximally elevated in the S phase of proliferating and transformed cells and is recognized by the monoclonal antibody PC-10 in paraffin tissue sections. The liver regenerative process after partial hepatectomy in rats was estimated with the in vivo incorporation of [3H]thymidine into liver DNA and the liver thymidine kinase activity. The expression of PCNA in rat liver after partial hepatectomy was performed by immunohistochemical staining with PC-10 in paraffin embedded tissues, at different time intervals up to 240 hr. Proliferating cell nuclear antigen expression, [3H]thymidine incorporation into DNA, and liver thymidine kinase activity exhibited marked oscillations during the liver regenerative process. A close relationship was demonstrated among DNA synthesis, thymidine kinase activity, and PC-10 score. Our results suggest that PC-10 monoclonal antibody may be used as a worthwhile proliferation index in the evaluation of the rate of liver regeneration in rats.
Theocharis
S E
SE
Department of Experimental Pharmacology, Medical School, University of Athens, Greece.
Skopelitou
A S
AS
Margeli
A P
AP
Pavlaki
K J
KJ
Kittas
C
C
D000818:Animals; D002455:Cell Division / Q000502:physiology; D006498:Hepatectomy; D007124:Immunoenzyme Techniques; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D017208:Rats, Wistar; D013937:Thymidine Kinase / Q000378:metabolism
D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D013937:Thymidine Kinase