Evaluation of the potency of allergenic extracts by inhibition of IgG-antibody binding.
A method is described for the in vitro potency evaluation of allergenic extracts by using their capacity of binding to specific human IgG antibodies. The results obtained with this inhibition-type enzyme immunoassay are compared with the analyses by the customary method of IgE antibody binding. A large series of allergenic protein fractions from the pollen of the Gramineae, Olea europea, Parietaria judaica, and from the dust mite Dermatophagoides pteronyssinus as well as from cat dander and peanuts were examined for inhibition of specific antibodies of both IgG and IgE isotypes. Potency evaluation by inhibition assays for IgE- and IgG-binding showed a significant correlation for the points of 50% inhibition (r = 0.92, p = 0.0001), but not for the slopes of the inhibition curves, i.e. the respective antibody avidities. Evidence is provided that the strict relationship between IgE- and IgG-inhibition by allergens could not be explained by possible cross-contaminations of the anti IgE- or anti IgG-reagents employed in the immunoassays. It is concluded that the inhibition of IgG-antibody binding presents a fast, reliable and low-cost alternative for the potency control of allergens used in clinical practice.
Boluda
L
L
Research Group C.B.F. LETI, Tres Cantos (Madrid), Spain.
Gallego
M T
MT
Gonzalez Romano
M L
ML
Cambra
O
O
Berrens
L
L
D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D001667:Binding, Competitive; D001688:Biological Products / Q000276:immunology / Q000302:isolation & purification / Q000592:standards; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007074:Immunoglobulin G / Q000145:classification / Q000378:metabolism*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D011058:Pollen / Q000276:immunology
D000485:Allergens; D001688:Biological Products; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007073:Immunoglobulin E