title: Evidence for a polymorphism of HLA-G gene.
abstract: HLA-G gene polymorphism was analyzed by RFLP using seven restriction enzymes and an HLA-G locus-specific probe. Hybridization of 55 DNAs digested with three enzymes (Taq I, Pst I, and Bgl II) revealed two polymorphic bands in each case. RFLP patterns obtained with Taq I and Pst I corresponded to the same allelic polymorphism and differed from the Bgl II polymorphism. Combining both polymorphisms enabled determination of four alleles. Allelic frequencies were calculated: 40% of the subjects tested had allele 1, 36% had allele 2, 22% had allele 3, and 2% had allele 4. Analyzing the complete HLA class I phenotype revealed strong linkage disequilibrium with the HLA-A locus. The polymorphism described is located in the 3' flanking region of the gene. Moreover, extended HLA-A haplotypes were constructed by combining the HLA-G polymorphism with other class-I-sequence polymorphisms.
authors.affiliation: Histocompatibility Laboratory, Regional Center for Blood Transfusion, Rennes, France.
authors.forename: M
authors.initials: M
authors.lastname: Alizadeh
authors.forename: C
authors.initials: C
authors.lastname: Legras
authors.forename: G
authors.initials: G
authors.lastname: Semana
authors.forename: P
authors.initials: P
authors.lastname: Le Bouteiller
authors.forename: B
authors.initials: B
authors.lastname: Genetet
authors.forename: R
authors.initials: R
authors.lastname: Fauchet
mesh_terms: D001483:Base Sequence; D005787:Gene Frequency / Q000502:physiology; D005805:Genes, MHC Class I; D006680:HLA Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I