Antigen retrieval using pH 3.5 glycine-HCl buffer or urea solution for immunohistochemical localization of Ki-67.
A new antibody (MIB-1) has been described, permitting the demonstration of Ki-67 proliferation antigen in paraffin sections. However, satisfactory results were obtained only after subjecting tissue sections to microwave based antigen retrieval in citrate buffer solution. Other buffer solutions produce equivalent or better results and also permit use of the original Ki-67 antibody, which hitherto has been considered ineffective for paraffin sections.
Shi
S R
SR
Department of Pathology, University of Southern California School of Medicine, Los Angeles 90033.
Chaiwun
B
B
Young
L
L
Imam
A
A
Cote
R J
RJ
Taylor
C R
CR
D002021:Buffers; D002467:Cell Nucleus / Q000737:chemistry; D002951:Citrates; D005557:Formaldehyde; D005998:Glycine / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry / Q000379:methods; D019394:Ki-67 Antigen; D008198:Lymph Nodes / Q000737:chemistry / Q000166:cytology; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000473:pathology; D008872:Microwaves; D009363:Neoplasm Proteins / Q000032:analysis* / Q000528:radiation effects; D009687:Nuclear Proteins / Q000032:analysis* / Q000528:radiation effects; D016612:Paraffin Embedding; D012996:Solutions; D016707:Tissue Fixation / Q000379:methods; D014508:Urea
D002021:Buffers; D002951:Citrates; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012996:Solutions; D005557:Formaldehyde; D014508:Urea; D006851:Hydrochloric Acid; D005998:Glycine