[Influence of cytokines on ICAM-1 expression and soluble ICAM-1 production by human endothelial cells].
The present study was designed to clarify (1) the effects of cytokines on intercellular adhesion molecule-1 (ICAM-1) expression on human endothelial cells (EC) and soluble ICAM-1 (sICAM-1) production by these cells and (2) the effects of purified sICAM-1 on leukocyte-EC adhesion. IL-1 beta and TNF-alpha induced a parallel increase of both the ICAM-1 expression on EC and sICAM-1 production by EC, while IFN-gamma induced only the dose dependent enhancement of ICAM-1 expression, but not the production of sICAM-1, IL-4 and IL-6 did not show any effects on the ICAM-1 expression nor on the sICAM-1 production. IL-4 and IL-6 abolished the expression enhancing effect by IL-1 beta, while TNF-alpha showed a synergism with IL-1 beta to promote ICAM-1 expression on EC. The sICAM-1 continued to accumulate in the culture supernatant even at 48 h after stimulation of EC by IL-1 beta, whereas the ICAM-1 expression on EC reached the maximum at 40 h and declined thereafter under the stimulation by IL-1 beta. IL-1 beta treatment of EC resulted in an increase in adhesion of leukocytes to the EC. The sICAM-1, purified from the cell-free supernatant obtained after a 48 h culture of EC in IL-1 beta by affinity column chromatography using monoclonal ICAM-1 antibody coupled to Sepharose beads, significantly inhibited leukocyte-EC adhesion.(ABSTRACT TRUNCATED AT 250 WORDS)
Hashimoto
M
M
Department of Clinical Immunology, Kyushu University, Beppu.
D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000378:metabolism* / Q000502:physiology; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D008214:Lymphocytes; D012995:Solubility
D015815:Cell Adhesion Molecules; D016207:Cytokines; D018799:Intercellular Adhesion Molecule-1