Parvalbumin-containing neurons in the cerebral cortex of the lizard Podarcis hispanica: morphology, ultrastructure, and coexistence with GABA, somatostatin, and neuropeptide Y.
The morphology, fine structure, and degree of colocalization with GABA, somatostatin, and neuropeptide Y of parvalbumin-containing cells were studied with immunocytochemistry in the cerebral cortex of the lizard Podarcis hispanica. Parvalbumin-containing cells make up a morphologically heterogeneous population of spine-free neurons, displaying the morphological features of nonprincipal cells previously described in Golgi studies. Electron microscopically, parvalbumin-immunoreactive cell bodies are similar in all cortical areas and layers. The perisomatic input is moderate in number, and boutons with either round clear vesicles or flattened vesicles were observed making asymmetric or symmetric synaptic contacts, respectively. Parvalbumin-immunoreactive dendrites are smooth and almost completely covered with synaptic boutons of different types, most of which establish asymmetric contacts. Parvalbumin-immunoreactive boutons are concentrated around cell bodies of principal cells. They are large, containing abundant mitochondria and small pleomorphic vesicles, and establishing symmetric synaptic contacts with somata, proximal dendritic shafts, and axon initial segments of principal cells. Colocalization studies revealed that all the parvalbumin-containing cells are GABA-immunoreactive, representing only a fraction of the GABA-immunopositive cell population, and that parvalbumin- and peptide- (somatostatin and neuropeptide Y) containing cells show a negligible overlap. These results demonstrate that in the cerebral cortex of the lizard Podarcis hispanica, parvalbumin-containing cells represent a subset of nonprincipal GABAergic neurons largely involved in perisomatic inhibition, which are different from the peptide-containing cells, and suggest that they may include both axosomatic and axoaxonic cells.
Martínez-Guijarro
F J
FJ
Biología Celular, Universidad de Valencia, Burjasot, Spain.
Soriano
E
E
Del Río
J A
JA
Blasco-Ibáñez
J M
JM
López-García
C
C
D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D008116:Lizards / Q000033:anatomy & histology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000737:chemistry*; D009478:Neuropeptide Y / Q000032:analysis; D010320:Parvalbumins / Q000032:analysis*; D013004:Somatostatin / Q000032:analysis; D013569:Synapses / Q000737:chemistry; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D010320:Parvalbumins; D013004:Somatostatin; D005680:gamma-Aminobutyric Acid