title: Characterization of uvi15+, a stress-inducible gene from Schizosaccharomyces pombe.
abstract: The uvi15+ gene of Schizosaccharomyces pombe is a member of a group of stress-inducible genes transcription levels of which increase in response to DNA-damaging agents or heat shock. It encodes a polypeptide of calculated molecular mass 11641 Da, with no significant sequence similarity to other known heat shock proteins. The steady-state level of the uvi15+ gene product of about 12 kDa was increased by heat shock and canavanine, an amino acid analog. This gene also showed a transient increase in expression as cells moved into diauxic shift phase. Although deletion of the uvi15+ gene did not affect the mitotic growth or thermotolerance of cells, the mutant cells rapidly lost viability in stationary phase and under starvation conditions. These cells also showed a defect in sporulation ability. These results suggest that the uvi15+ gene encodes a stress response protein involved in the maintenance of cell viability during entry into stationary phase or under starvation conditions.
authors.affiliation: Department of Molecular Biology, Seoul National University, Korea.
authors.forename: J K
authors.initials: JK
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Kim
authors.forename: J
authors.initials: J
authors.lastname: Choe
authors.forename: R H
authors.initials: RH
authors.lastname: Seong
authors.forename: S H
authors.initials: SH
authors.lastname: Hong
authors.forename: S D
authors.initials: SD
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects; D005800:Genes, Fungal / Q000528:radiation effects*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012568:Schizosaccharomyces / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays