Testis and somatic Xrcc-1 DNA repair gene expression.
The human XRCC1 gene has been shown to be involved in DNA strand-break repair using the Chinese hamster ovary cell mutant EM9. The purpose of this study was to characterize the expression of Xrcc-1 to determine if there is tissue-specific expression and to provide a baseline of information for future studies that may involve altering Xrcc-1 expression in mice. Normal young adult male testis and enriched populations of pachytene spermatocytes and round spermatids displayed significantly higher levels of Xrcc-1 expression than other mouse tissues, although Xrcc-1 transcripts were found in low abundance in all tested tissues. Cultured mouse cell lines displayed levels of expression similar to male germ cells, which is a striking contrast to the levels of expression obtained in somatic tissues from the mouse. The relatively high levels of expression identified in male germ cells indicate Xrcc-1 may have an important role in male germ cell physiology.
Walter
C A
CA
Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio 78284-7762.
Lu
J
J
Bhakta
M
M
Zhou
Z Q
ZQ
Thompson
L H
LH
McCarrey
J R
JR
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D004260:DNA Repair / Q000235:genetics*; D015870:Gene Expression; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D013087:Spermatids / Q000648:ultrastructure; D013090:Spermatocytes / Q000648:ultrastructure; D013737:Testis / Q000378:metabolism*