Synthesis, properties, and intratumoral evaluation of mitoxantrone-loaded casein microspheres in Lewis lung carcinoma.
Smooth, round, uniform bovine casein microspheres of 1-5 and 10-20 microns size were readily prepared by a steric stabilization technique previously developed in this laboratory for synthesis of albumin microspheres. The avid phagocytic uptake of casein and albumin microspheres was demonstrated with fluorescein-labelled microspheres using a macrophage-like mouse myelomonocytic leukaemia cell line. Post-synthesis loading of 25% mitoxantrone was achieved for casein microspheres containing 20% polyglutamic acid. Preliminary intratumoural chemotherapy experiments with a mouse Lewis lung carcinoma indicated that mitoxantrone and mitoxantrone-loaded casein-polyglutamic acid microspheres exhibited lower toxicity when administered intratumorally.
Knepp
W A
WA
Department of Material Science, University of Florida, Gainesville 32611.
Jayakrishnan
A
A
Quigg
J M
JM
Sitren
H S
HS
Bagnall
J J
JJ
Goldberg
E P
EP
D000818:Animals; D002364:Caseins / Q000737:chemistry*; D015552:Injections, Intralesional; D008175:Lung Neoplasms / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008863:Microspheres; D008942:Mitoxantrone / Q000008:administration & dosage*; D009368:Neoplasm Transplantation; D010587:Phagocytosis / Q000187:drug effects; D012710:Serum Albumin, Bovine; D014407:Tumor Cells, Cultured
D002364:Caseins; D012710:Serum Albumin, Bovine; D008942:Mitoxantrone