[Detection of translocation (15;17) using cytogenetic and molecular techniques. Comparative study in 15 patients with acute promyelocytic leukemia].
OBJECTIVE
To analyze the reproducibility to detect the t (15; 17) with molecular and cytogenetic techniques.


METHODS
Fifteen cases of acute promyelocytic leukaemia were studied by means of cytogenetic techniques (GTG banding) and molecular ones (K/S probe and HindIII, EcoRI and BamHI restriction enzymes).


RESULTS
The t (15; 17) was detected with cytogenetic techniques in 60% cases and with molecular ones in 33% cases. Using both (cytogenetic and molecular) techniques the translocation was detected in 73% cases.


CONCLUSIONS
The use of both techniques may be very useful in order to get a better diagnostic and a closer control of the patient's clinical evolution.
Arranz
E
E
Departamento de Genética y Hematología, Fundación Jiménez Díaz, Madrid.
Benítez
J
J
Santón
A
A
Portero
J A
JA
Sánchez-Fayos
J
J
D002871:Chromosome Banding; D002884:Chromosomes, Human, Pair 15 / Q000648:ultrastructure*; D002886:Chromosomes, Human, Pair 17 / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000473:pathology; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D014178:Translocation, Genetic
D004273:DNA, Neoplasm
Detección de la translocación (15; 17) con técnicas citogenéticas y moleculares. Estudio comparativo en 15 pacientes con leucemia aguda promielocítica.