Influence of staphylococcal lipoteichoic acid on the frustrated phagocytosis of neutrophils against opsonized corneocytes.
Stratum corneum (SC) exerts a proinflammatory effect in the presence of complement. When Staphylococcus aureus (S. aureus) invades the skin through damaged SC, neutrophils accumulate at the subcorneal portion of epidermis to phagocytize the S. aureus as noted in impetigo. Besides the phagocytosis of bacteria, neutrophils interact with opsonized SC in a form of frustrated phagocytosis, increasing a damage of the surrounding tissues. Based on our previous finding that staphylococcal protein A promotes the interaction between SC and neutrophils, we investigated whether lipoteichoic acid (LTA), another cell wall component of S. aureus, also shows similar properties. We found that LTA significantly promoted the binding of neutrophils to opsonized SC, resulting in an increase in SC-induced respiratory burst of neutrophils assessed by chemiluminescence (CL). The binding of neutrophils to the SC was almost completely inhibited by the blocking of CR3 with anti-CD11b antibody, suggesting that the binding between SC and neutrophils is mediated by interaction between C3bi and CR3 (Mac-1). Such enhanced interaction seems to function in the primary host defence mechanism against the invading S. aureus through the skin such as in impetigo.
Kato
T
T
Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
Terui
T
T
Zhen
Y X
YX
Tagami
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D015703:Antigens, CD / Q000276:immunology; D018845:CD11 Antigens; D002473:Cell Wall / Q000737:chemistry; D003179:Complement C3b / Q000502:physiology*; D003170:Complement Pathway, Alternative; D000078404:Epidermal Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D008163:Luminescent Measurements; D016177:Macrophage-1 Antigen / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects*; D011951:Receptors, Complement / Q000276:immunology / Q000502:physiology*; D016897:Respiratory Burst / Q000187:drug effects; D013207:Staphylococcal Skin Infections / Q000503:physiopathology; D013211:Staphylococcus aureus / Q000737:chemistry*; D013682:Teichoic Acids / Q000302:isolation & purification / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018845:CD11 Antigens; D008070:Lipopolysaccharides; D016177:Macrophage-1 Antigen; D009895:Opsonin Proteins; D011951:Receptors, Complement; D013682:Teichoic Acids; C009900:lipoteichoic acid; D003179:Complement C3b