Investigation of hospital-acquired infections due to Alcaligenes denitrificans subsp. xylosoxydans by DNA restriction fragment length polymorphism.
We demonstrate that DNA restriction fragment length polymorphism determined by pulsed-field gel electrophoresis is very useful in the investigation of the epidemiology of hospital-acquired infections caused by Alcaligenes denitrificans subsp. xylosoxydans. This approach showed that hospital-acquired infections caused by this opportunistic pathogen over a 6-month period in 10 patients hospitalized in an intensive care unit and a surgical unit were not a true outbreak. In addition, this molecular typing method established that the respiratory therapy equipment was the source of the contamination of two patients.
Cheron
M
M
Laboratoire de Bactériologie-Virologie, Hôpital Boucicaut, Paris, France.
Abachin
E
E
Guerot
E
E
el-Bez
M
M
Simonet
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000421:Alcaligenes / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D003428:Cross Infection / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology; D006801:Humans; D007362:Intensive Care Units; D008297:Male; D008875:Middle Aged; D009894:Opportunistic Infections / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology; D010297:Paris / Q000453:epidemiology; D011014:Pneumonia / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D012138:Respiratory Therapy / Q000009:adverse effects / Q000295:instrumentation
D004269:DNA, Bacterial