Screening cosmid libraries with oligonucleotides corresponding to splice-site consensus sequences.
To facilitate the identification of genes within genomic DNA, we have developed a method based on the use of short oligonucleotides designed from the consensus sequences of splice sites. We describe here the hybridization and washing conditions under which such oligonucleotides can be used to screen cosmid libraries. We confirm the presence of genes within cosmids identified by screening with one oligonucleotide by showing that DNA isolated from such cosmids will hybridize to another splice-site oligonucleotide.
Melmer
G
G
Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
Buchwald
M
M
D000818:Animals; D016384:Consensus Sequence; D003360:Cosmids / Q000235:genetics*; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005821:Genetic Techniques; D006801:Humans; D006822:Hybrid Cells; D009841:Oligonucleotides / Q000235:genetics*; D012326:RNA Splicing
D009841:Oligonucleotides; D004247:DNA