Hybridization of clinical Escherichia coli isolates from calves and piglets in New York State with gene probes for enterotoxins (STaP, STb, LT), Shiga-like toxins (SLT-1, SLT-II) and adhesion factors (K88, K99, F41, 987P).
Six hundred and sixty-six bovine and fifty-seven swine clinical isolates of E. coli from New York state were examined for the presence of enterotoxins (STaP, STb, LT, SLT-I, and SLT-II) and adhesins (K88, K99, F41, and 987P) using colony hybridization techniques. Three hundred and sixty-seven of the bovine isolates (45.2%) hybridized with at least one gene probe. Of these, two hundred and twenty-three (33.2%) hybridized with F41, one hundred twelve (16.7%) with K99, eighty-two (12.2%) with 987P, ninety-six (14.3%) with STaP, seven (1.1%) with STb, and none (0.0%) with LT and K88. A total of thirty-three (4.7%) of the isolates hybridized with SLT-I, and one (0.1%) with SLT-II. The major pathotypes among the 666 isolates from bovine were K99/F41/StaP (9.8%), K99/F41 (2.5%), p87P/F41 (2.1%) and 987P/K99/F41/StaP (1.4%). Of the swine clinical isolates, twenty-two hybridized with at least one gene probe. The major pathotypes among the isolates from piglets were K88/K99/F41/StaP (5.3%) and K88/F41 (5.3%).
Shin
S J
SJ
Diagnostic Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, NY.
Chang
Y F
YF
Timour
M
M
Lauderdale
T L
TL
Lein
D H
DH
D018830:Adhesins, Escherichia coli; D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases; D015342:DNA Probes; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005798:Genes, Bacterial; D009518:New York; D010957:Plasmids; D015183:Restriction Mapping; D013552:Swine; D013553:Swine Diseases
D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D015342:DNA Probes; D004768:Enterotoxins