Assignment of the human gene for the alpha 1 subunit of the skeletal muscle DHP-sensitive Ca2+ channel (CACNL1A3) to chromosome 1q31-q32.
A human clone corresponding to the gene encoding the alpha 1 subunit of the skeletal muscle dihydropyridine-sensitive calcium channel (CACNL1A3) has been isolated and partially sequenced. Oligonucleotides based on this sequence were used in a polymerase chain reaction to amplify specifically the human gene in human-rodent somatic cell hybrids, allowing the assignment of CACNL1A3 to chromosome 1. A polymorphic dinucleotide repeat also was identified in the human clone and using PCR was typed on a subset of the CEPH families. Multipoint linkage analysis places the CACNL1A3 gene between D1S52 and D1S70, on chromosome 1q31-q32.
Gregg
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RG
Department of Anesthesiology, University of Wisconsin, Madison 53705.
Couch
F
F
Hogan
K
K
Powers
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015220:Calcium Channels / Q000187:drug effects / Q000235:genetics*; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA; D004095:Dihydropyridines / Q000494:pharmacology; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry*; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D015220:Calcium Channels; D004095:Dihydropyridines; C038806:1,4-dihydropyridine; D004247:DNA