title: Humoral and cellular immune responses to the fimbriae of Porphyromonas gingivalis and their synthetic peptides.
abstract: Subcutaneous injection of fimbriae from Porphyromonas gingivalis strain 381 in Freund's incomplete adjuvant (FIA) resulted in an excellent serum anti-fimbrial immunoglobulin G (IgG) response in guinea-pigs and BALB/c mice. Administration of P. gingivalis fimbriae also elicited distinct cellular immune responses to the fimbriae in terms of ear lobe reaction in BALB/c but not in BALB/c nu/nu mice, and of skin reaction in guinea-pigs. When the guinea-pigs were given a semi-synthetic adjuvant GM-53--sodium beta-N-acetylglycosaminyl-(1-->4)-N-acetylmuramyl-L-alanyl-D-isogl utaminyl- (L)-stearoyl-(D)-meso-2, 6-diaminopimelic acid-(D)-amide-D-alanine--and fimbriae in FIA by subcutaneous injection, more enhanced production of serum anti-fimbrial IgG and stronger cellular immune responses were induced in the guinea-pigs than in those given fimbriae alone. Synthetic peptide FP381(202-221), which corresponds to the amino-acid residue numbers 202-221 based on the amino-acid sequence of fimbrilin from P. gingivalis strain 381, elicited humoral and cellular immune responses in guinea-pigs immunised with the fimbriae or FP381(202-221). Furthermore, subcutaneous administration of synthetic peptide FP381(61-80) with GM-53 induced lesser degrees of humoral and cellular immune responses in guinea-pigs than did FP381(202-221). However, when the fimbriae or FP381(61-80) were administered with bovine serum albumin (BSA), markedly elevated levels of specific anti-BSA antibody were seen in the serum of BALB/c mice. These results clearly indicated that fimbriae from P. gingivalis 381 and their oligopeptide segments induced humoral and cellular immune responses and exhibited immuno-adjuvant activities in guinea-pigs and BALB/c mice.
authors.affiliation: Department of Oral Microbiology, Osaka University Faculty of Dentistry, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: H
authors.initials: H
authors.lastname: Ogo
authors.forename: H
authors.initials: H
authors.lastname: Uchida
authors.forename: S
authors.initials: S
authors.lastname: Hamada
mesh_terms: D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000276:immunology*; D006168:Guinea Pigs; D007111:Immunity, Cellular; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008969:Molecular Sequence Data; D016966:Porphyromonas gingivalis / Q000276:immunology* / Q000648:ultrastructure; D012867:Skin / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; C063055:fimbrillin; D036781:Fimbriae Proteins