title: Comparative toxicity of amyloid beta-peptide in neuroblastoma cell lines: effects of albumin and physalaemin.
abstract: 1. Synthetic amyloid beta-peptide was toxic to NB41A3 neuroblastoma cells in serum-free culture as judged by decreasing cell numbers and release of the cytosolic enzyme, lactic dehydrogenase. 2. Without amyloid beta-peptide, bovine serum albumin increased the number of cells surviving in culture. 3. In the presence of amyloid beta-peptide, BSA appeared to potentiate the amyloid beta-peptide toxicity. 4. The toxic dose response for amyloid beta-peptide varied between different cell lines (NB41A3, NB2a and IMR32), in a range of 100-1000 nM amyloid beta-peptide. 5. Amyloid beta-peptide toxicity was inhibited by the concurrent treatment of the cells with the tachykinin physalaemin with an ED50 of 10(-6) M.
authors.affiliation: Department of Chemistry, Institute of Arctic Biology, University of Alaska Fairbanks.
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: J A
authors.initials: JA
authors.lastname: Valantas
authors.forename: S
authors.initials: S
authors.lastname: Vyas
authors.forename: L K
authors.initials: LK
authors.lastname: Duffy
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D016895:Culture Media, Serum-Free; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D010803:Physalaemin / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D016229:Amyloid beta-Peptides; D016895:Culture Media, Serum-Free; D010803:Physalaemin; D012710:Serum Albumin, Bovine; D007770:L-Lactate Dehydrogenase