Cloning of human and rat p69 cDNA, a candidate autoimmune target in type 1 diabetes.
Triggering of autoimmunity in insulin-dependent diabetes was linked to dietary bovine serum albumin (BSA). Anti-BSA antibodies from diabetes-prone rats precipitate a protein, p69, from islet cell lysates. We have used these antibodies to identify rat p69 cDNAs. Human p69 cDNA was identified by crosshybridization. The p69 coding regions show 87% nucleotide and 89% amino acid homology. Recombinant p69 is recognized by autoantibody and T cells from diabetic children.
Miyazaki
I
I
Hospital for Sick Children, Department of Paediatrics, University of Toronto, Ontario, Canada.
Gaedigk
R
R
Hui
M F
MF
Cheung
R K
RK
Morkowski
J
J
Rajotte
R V
RV
Dosch
H M
HM
D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000235:genetics* / Q000276:immunology; D015551:Autoimmunity; D001483:Base Sequence; D002648:Child; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011913:Rats, Inbred BB; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012710:Serum Albumin, Bovine / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D001324:Autoantigens; D018076:DNA, Complementary; C087054:ICA1 protein, human; C490185:Ica1 protein, rat; D012333:RNA, Messenger; D012710:Serum Albumin, Bovine