Frequent allelic deletion at a novel locus on chromosome 5 in human lung cancer.
Frequent allelic deletions in tumor cells are indicative of the inactivation of tumor suppressor genes. Recently, we isolated the single-copy sequence del-27 (I. Wieland, M. Böhm, and S. Bogatz, Proc. Natl. Acad. Sci. USA, 89: 9705-9709, 1992). Here we show that del-27 detects a restriction fragment length polymorphism that allows examination for loss of heterozygosity (LOH) in tumor specimens. LOH at the del-27 locus occurred in 57% (4 of 7) of the informative lung carcinomas independent of the histopathological differentiation grade. LOH for exon 11 of the APC gene occurred in 71% (5 of 7) of the informative cases but was not associated with LOH at the del-27 locus. The del-27 sequence was localized to chromosome 5p13-5q14, proximal to the MCC/APC region, using a somatic cell hybrid panel. Together with our previous finding that del-27 is deleted homozygously in a lung carcinoma cell line, these results suggest that del-27 is linked closely to a novel putative tumor suppressor gene.
Wieland
I
I
Institut für Zellbiologie (Tumorforschung), Universitätsklinikum Essen, Germany.
Böhm
M
M
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D017931:DNA Primers; D015247:Deoxyribonuclease HindIII; D017353:Gene Deletion; D017491:Genes, APC; D016147:Genes, Tumor Suppressor; D006801:Humans; D006822:Hybrid Cells; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D015247:Deoxyribonuclease HindIII