Nicotine-induced regulation of tyrosine hydroxylase activity in adrenal gland of transgenic mouse carrying human tyrosine hydroxylase gene.
We investigated the effect of subcutaneous injection of nicotine on in vitro tyrosine hydroxylase (TH) activity in adrenal gland and brain of the transgenic mice carrying an 11-kb fragment containing the entire human TH gene. Injection of 5 mg nicotine/kg (as free base) for 3 days caused a statistically significant increase in vitro TH activity in the adrenal gland, whereas brain TH activity was not affected at all. The adrenal gland of non-transgenic C57BL/6J mice treated in the same way as for transgenic mice tended to enhance TH activity, although not to a significant level. This observation might indicate the possibility that the machinery used by nicotine in regulating the properties or expression of TH in the adrenal gland should be similar between transgenic and non-transgenic mice.
Ota
A
A
Joint Research Division for Therapies against Intractable Diseases, School of Medicine, Fujita Health University, Aichi, Japan.
Ichinose
H
H
Kobayashi
K
K
Morita
S
S
Sawada
H
H
Mizuguchi
T
T
Nagatsu
T
T
D000311:Adrenal Glands / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D004759:Enterochromaffin Cells / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009538:Nicotine / Q000494:pharmacology*; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis* / Q000235:genetics*
D009538:Nicotine; D014446:Tyrosine 3-Monooxygenase