title: Isolation and mapping of a cosmid clone containing the human NAT2 gene.
abstract: The NAT2 gene encodes for a polymorphic arylalkylamine N-acetyltransferase and thus accounts for the human N-acetylation polymorphism. By a NAT2-specific primer set we have screened a human chromosome 8-specific cosmid library. A positive cosmid clone was mapped by fluorescence in situ hybridization to 8p22. The polymerase chain reaction followed by restriction analysis of the PCR product was used to identify allele 2 to be contained in the cosmid clone.
authors.affiliation: Center for Human Genetics and Genetic Counselling, University of Bremen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Franke
authors.forename: I
authors.initials: I
authors.lastname: Klawitz
authors.forename: E
authors.initials: E
authors.lastname: Schnakenberg
authors.forename: B
authors.initials: B
authors.lastname: Rommel
authors.forename: W
authors.initials: W
authors.lastname: Van de Ven
authors.forename: J
authors.initials: J
authors.lastname: Bullerdiek
authors.forename: W
authors.initials: W
authors.lastname: Schloot
mesh_terms: D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D003360:Cosmids; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D017931:DNA Primers; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human