Detection of hepatitis C virus RNA by in situ hybridization: a critical appraisal.
Despite the several papers that have appeared in the literature or have been communicated at scientific meetings, the detection of hepatitis C virus RNA by in situ hybridization seems a difficult goal to achieve. There have been conflicting reports on the type and proportion of hepatitis C virus-infected cells, the intracellular distribution of viral RNA and the topographical association with cell damage. As a consequence, some of the findings should probably be considered as non-specific and all protocols and data critically reviewed before a firm conclusion be made.
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Department of Gastroenterology, Ospedale Molinette, Turin, Italy.
D000818:Animals; D016174:Hepacivirus / Q000235:genetics*; D017403:In Situ Hybridization / Q000379:methods*; D002679:Pan troglodytes; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000302:isolation & purification*
D012367:RNA, Viral