title: Lipid synthesis in mycobacteria: characterization of the biotin carboxyl carrier protein genes from Mycobacterium leprae and M. tuberculosis.
abstract: The causative agents of leprosy and tuberculosis, Mycobacterium leprae and Mycobacterium tuberculosis, have a lipid-rich cell envelope which contributes to virulence and antibiotic resistance. Acyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis, consists in mycobacteria of two subunits, one of which is biotinylated. Genes from M. leprae and M. tuberculosis encoding a biotinylated protein have been cloned and sequenced. Analysis of the derived protein sequences demonstrated the presence of biotin-binding sites and putative ATP-bicarbonate interactions sites, consistent with the proteins having a biotin carboxylase function as well as their being biotin carrier proteins.
authors.affiliation: Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, United Kingdom.
authors.forename: E
authors.initials: E
authors.lastname: Norman
authors.forename: K A
authors.initials: KA
authors.lastname: De Smet
authors.forename: N G
authors.initials: NG
authors.lastname: Stoker
authors.forename: C
authors.initials: C
authors.lastname: Ratledge
authors.forename: P R
authors.initials: PR
authors.lastname: Wheeler
authors.forename: J W
authors.initials: JW
authors.lastname: Dale
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001710:Biotin / Q000032:analysis; D002352:Carrier Proteins / Q000235:genetics*; D015342:DNA Probes; D054889:Fatty Acid Synthase, Type II; D005798:Genes, Bacterial / Q000235:genetics*; D008055:Lipids / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D015342:DNA Probes; D008055:Lipids; D001710:Biotin; D054889:Fatty Acid Synthase, Type II; D000103:Acetyl-CoA Carboxylase; C079633:biotin carboxyl carrier protein