title: HIV-1/HIV-2 seronegativity in HIV-1 subtype O infected patients.
abstract: Nine patients with atypical HIV-1 western blot profiles were diagnosed as having HIV-1 subtype O infection. All the patients were living in France; eight originated from Cameroon and one from France. Lymphocyte DNA amplification by the polymerase chain reaction was only positive when HIV-1 subtype O specific primers were used. Preliminary sequence analysis of amplified products and serological reactivity against a specific subtype O synthetic env peptide confirmed HIV-1 subtype O infection. HIV-1/HIV-2 enzyme-linked immunosorbent assays, especially those based on env peptides or on the sandwich format, can be negative in HIV-1 subtype O infection.
authors.affiliation: Virology Department, Bichat-Claude Bernard Hospital, Paris, France.
authors.forename: I
authors.initials: I
authors.lastname: Loussert-Ajaka
authors.forename: T D
authors.initials: TD
authors.lastname: Ly
authors.forename: M L
authors.initials: ML
authors.lastname: Chaix
authors.forename: D
authors.initials: D
authors.lastname: Ingrand
authors.forename: S
authors.initials: S
authors.lastname: Saragosti
authors.forename: A M
authors.initials: AM
authors.lastname: Couroucé
authors.forename: F
authors.initials: F
authors.lastname: Brun-Vézinet
authors.forename: F
authors.initials: F
authors.lastname: Simon
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis / Q000382:microbiology*; D000328:Adult; D015153:Blotting, Western; D002163:Cameroon; D004797:Enzyme-Linked Immunosorbent Assay; D005602:France; D005784:Gene Amplification; D018023:HIV Seronegativity; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000276:immunology; D015498:HIV-2 / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction