Transcriptional regulation of the PCNA promoter by p53.
We have examined the ability of p53 to affect transcription from the PCNA promoter in a number of cell types. In HeLa cells, p53 activates the PCNA promoter whereas in CV1, CHO, L929, and Saos-2 cells the same promoter is strongly repressed. By using stepwise deletions of the PCNA promoter, we have identified two potential regions of the promoter which are important for the ability of p53 to activate transcription in HeLa cells.
Jackson
P
P
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra.
Ridgway
P
P
Rayner
J
J
Noble
J
J
Braithwaite
A
A
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D006224:Cricetinae; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D007739:L Cells; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics*; D010957:Plasmids; D018809:Proliferating Cell Nuclear Antigen; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53; D015500:Chloramphenicol O-Acetyltransferase