title: Prenatal diagnosis of congenital human cytomegalovirus infection.
abstract: Fifteen fetuses at risk of congenital human cytomegalovirus (HCMV) infection underwent prenatal diagnosis at 16-30 weeks' gestation by a combination of amniocentesis and fetal blood sampling. HCMV was isolated from the amniotic fluid in six patients, but HCMV-specific IgM was detected in only three of them. Two of the nine neonates, who were delivered following a negative prenatal diagnosis, had congenital HCMV infection diagnosed by virus isolation in the urine. The interval from infection to prenatal testing was 3 and 4 weeks in the two false-negative cases and > or = 7 weeks in the true-positive cases. Although timely testing for HCMV infection allows the option of termination of pregnancy, it may be flawed by false-negative results.
authors.affiliation: First Department of Obstetrics and Gynaecology, University of Milano, Italy.
authors.forename: U
authors.initials: U
authors.lastname: Nicolini
authors.forename: A
authors.initials: A
authors.lastname: Kustermann
authors.forename: B
authors.initials: B
authors.lastname: Tassis
authors.forename: R
authors.initials: R
authors.lastname: Fogliani
authors.forename: A
authors.initials: A
authors.lastname: Galimberti
authors.forename: E
authors.initials: E
authors.lastname: Percivalle
authors.forename: M
authors.initials: M
authors.lastname: Grazia Revello
authors.forename: G
authors.initials: G
authors.lastname: Gerna
mesh_terms: D000649:Amniocentesis; D000653:Amniotic Fluid / Q000821:virology; D000914:Antibodies, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000097:blood / Q000151:congenital* / Q000175:diagnosis*; D005188:False Negative Reactions; D005260:Female; D005312:Fetal Blood / Q000201:enzymology / Q000276:immunology; D005315:Fetal Diseases / Q000097:blood / Q000175:diagnosis*; D005865:Gestational Age; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007231:Infant, Newborn; D011247:Pregnancy; D011296:Prenatal Diagnosis; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M; D005723:gamma-Glutamyltransferase