Characterization and sequence of a 33-kDa enterohemolysin (Ehly 1)-associated protein in Escherichia coli.
The nucleotide sequence of the 2.1-kb EcoRI-AccI fragment of the enterohemolysin (Ehly)-associated plasmid, pEO21, has been determined. A third of this sequence encodes a 29.6-kDa protein, and coincides with the location of Tn1725 insertions which inactivate the production of Ehly. A protein of similar size (33-35 kDa) was found to be produced in large amounts by JM83[pEO21] and found to be immunologically related to the approximately 65-kDa protein made by the parental strain, C3888. DNA sequences coding for the 29.6-kDa protein of phi C3888, now designated Ehly 1, were found only in some enterohemolytic Escherichia coli indicating the existence of multiple, genetically different Ehlys.
Stroeher
U H
UH
Department of Microbiology and Immunology, University of Adelaide, Australia.
Bode
L
L
Beutin
L
L
Manning
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000378:metabolism*; D007106:Immune Sera; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009693:Nucleic Acid Hybridization; D011817:Rabbits; D015183:Restriction Mapping
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D007106:Immune Sera; C066219:enterohemolysin; C083758:enterohemolysin-associated protein, E coli