title: Localisation of alpha(2,3) and alpha(2,6) linked terminal sialic acid groups in human trabecular meshwork.
abstract: Sialic acid specific lectins were used to localise isomers of sialyl glycosides in human trabecular meshwork (TM) at the ultrastructural level. A lectin immunogold method demonstrated that sialic groups were concentrated on the endothelial surface of Schlemm's canal (SC) and in the adjacent juxta-canalicular tissue (JCT). One sialyl glycoside, alpha(2,6) linked N-acetyl neuraminic acid, was present mainly on the luminal aspect of the SC endothelium and in the cytoplasm of the JCT cells. Another, alpha(2,3) linked N-acetyl neuraminic acid, was localised predominantly to the extracellular fibrillar material of the JCT. The existence of a topographical segregation of these two sialyl glycosides within the TM supports the view that highly charged anionic molecules may be of significance in regulating aqueous outflow.
authors.affiliation: Department of Pathological Sciences, University of Manchester.
authors.forename: S A
authors.initials: SA
authors.lastname: Chapman
authors.forename: R E
authors.initials: RE
authors.lastname: Bonshek
authors.forename: R W
authors.initials: RW
authors.lastname: Stoddart
authors.forename: K R
authors.initials: KR
authors.lastname: Mackenzie
authors.forename: D
authors.initials: D
authors.lastname: McLeod
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001710:Biotin; D006801:Humans; D037102:Lectins; D008875:Middle Aged; D010835:Phytohemagglutinins; D037121:Plant Lectins; D054788:Ribosome Inactivating Proteins; D012794:Sialic Acids / Q000032:analysis*; D014129:Trabecular Meshwork / Q000737:chemistry*
chemical_list: D037102:Lectins; C042962:Limax flavus agglutinin; D010835:Phytohemagglutinins; D037121:Plant Lectins; C050600:Sambucus nigra lectins; D012794:Sialic Acids; C100156:leukoagglutinins, plants; D001710:Biotin; D054788:Ribosome Inactivating Proteins