Detection of P-glycoprotein in solid tumors by flow cytometry.
P-glycoprotein (P-gp) detection by FCM (flow cytometry) was correlated with P-gp detection by immunohistochemical staining or Western blotting in K562 cell lines variably resistant to doxorubicin (DOX). A strong correlation was noted between the flow cytometric data and the degree of resistance assessed by thymidine incorporation assay (TIA). Of the human carcinomas tested, 37.5% were P-gp positive by flow cytometry. The flow cytometry data correlated strongly with the immunohistochemical staining (p < 0.001). Thirteen of the 15 P-gp positive tumors were resistant to DOX and vincristine (VCR) on TIA. Thus, P-gp detection by FCM correlates strongly with tumor sensitivity to DOX and VCR (p < 0.001).
Fujii
H
H
Second Department of Surgery, Fukui Medical School, Japan.
Tanigawa
N
N
Muraoka
R
R
Shimomatsuya
T
T
Tanaka
T
T
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000032:analysis*; D002455:Cell Division / Q000187:drug effects; D004317:Doxorubicin / Q000633:toxicity; D004351:Drug Resistance; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D008562:Membrane Glycoproteins / Q000032:analysis*; D009369:Neoplasms / Q000737:chemistry / Q000473:pathology*; D013274:Stomach Neoplasms / Q000737:chemistry / Q000473:pathology; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014750:Vincristine / Q000633:toxicity
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D014750:Vincristine; D004317:Doxorubicin; D013936:Thymidine