title: Poly(ADP-ribose) catabolism in mammalian cells.
abstract: Poly(ADP-ribose) catabolism is a complex situation involving many proteins and DNA. We have developed an in vitro turnover system where poly(ADP-ribose) metabolism is monitored in presence of different relative amounts of two principal enzymes poly(ADP-ribose) transferase and poly(ADP-ribose) glycohydrolase along with other proteins and DNA. Our current results reviewed here show that the quality of polymer, i.e. chain length and complexity, as well as preference for the nuclear substrate varies depending upon the availability of poly(ADP-ribose) glycohydrolase. These results are interpreted in the light of the recent data implicating poly(ADP-ribose) metabolism in DNA-repair.
authors.affiliation: Molecular Endocrinology Research Center, CHUL Research Center, Laurier, Ste-Foy, Québec, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Lagueux
authors.forename: G M
authors.initials: GM
authors.lastname: Shah
authors.forename: L
authors.initials: L
authors.lastname: Ménard
authors.forename: H
authors.initials: H
authors.lastname: Thomassin
authors.forename: C
authors.initials: C
authors.lastname: Duchaine
authors.forename: C
authors.initials: C
authors.lastname: Hengartner
authors.forename: G G
authors.initials: GG
authors.lastname: Poirier
mesh_terms: D004257:DNA Polymerase II / Q000378:metabolism*; D004260:DNA Repair; D010446:Peptide Fragments / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D011065:Poly(ADP-ribose) Polymerases; D004257:DNA Polymerase II