Effect of low temperatures on the transfer of phospholipids with various acyl-chain lengths to the plasma membrane of leek cells.
The intracellular transport of lipids with very-long-chain acyl moieties (C20-C24 fatty acid-containing lipids) to the plasma membrane of leek cells follows the vesicular ER-Golgi apparatus-plasma membrane pathway. Here we report on the effect of low temperatures on the vesicular transport of lipids and especially C20-C24 fatty acid-containing lipids to the plasma membrane of leek cells. These lipids, normally transported through the ER-Golgi apparatus-plasma membrane pathway at 24 degrees C, accumulated in the ER and the Golgi apparatus at 12 degrees C with a related deficit in the plasma membrane. As lipids with long chain acyl moieties (C16 and C18 fatty acid-containing lipids) were still transferred to the plasma membrane at 12 degrees C, the data demonstrate a specific effect of low temperatures on the vesicular transfer of C20-C24 fatty acid-containing lipids to the plasma membrane of leek cells. Therefore, evidence is provided for distinct pathways and/or mechanisms transferring lipids to the plasma membrane of leek cells which differ in their sensitivity to low temperatures.
Moreau
P
P
URA 1811 CNRS, IBGC, University of Bordeaux II, France.
Sturbois
B
B
Morré
D J
DJ
Cassagne
C
C
D002462:Cell Membrane / Q000378:metabolism; D003080:Cold Temperature; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D006056:Golgi Apparatus / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D010944:Plants / Q000378:metabolism* / Q000648:ultrastructure
D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids