Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain.
Subtractive library construction and differential screening were used to identify a cDNA for a cell type-specific immediate early gene induced in rat PC12 pheochromocytoma cells. Sequencing identified the protein product of this gene as rat synaptotagmin IV (SytIV). Synaptotagmins are synaptic vesicle proteins thought to play a role in depolarization-induced, calcium-mediated exocytosis and neurotransmitter release. SytIV mRNA accumulation is transiently induced in PC12 cells by potassium depolarization, calcium ionophore, ATP, and forskolin. In contrast, growth factors and phorbol 12-myristate 13-acetate induce little or no SytIV mRNA accumulation. Kainic acid-induced seizures in rats are followed by accumulation of SytIV message in the hippocampus and piriform cortex. The SytIV gene may provide a direct link between depolarization-induced neuronal gene expression and subsequent modulation of synaptic structure and function.
Vician
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Department of Biological Chemistry, University of California, Los Angeles 90024.
Lim
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IK
Ferguson
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Tocco
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Baudry
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Herschman
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HR
D000310:Adrenal Gland Neoplasms; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D017931:DNA Primers; D015870:Gene Expression / Q000187:drug effects; D015723:Gene Library; D017781:Genes, Immediate-Early; D006624:Hippocampus / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D008564:Membrane Potentials; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D016716:PC12 Cells; D010673:Pheochromocytoma; D016133:Polymerase Chain Reaction; D011188:Potassium / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D050857:Synaptotagmins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D017931:DNA Primers; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; C494293:Syt4 protein, mouse; C494294:Syt4 protein, rat; D050857:Synaptotagmins; D005576:Colforsin; D013755:Tetradecanoylphorbol Acetate; D011188:Potassium; D007608:Kainic Acid