Effects of low-doses of X-rays on the expression of human cell surface CD2 antigen in CD2+ CHO cells.
Thioguanine resistant CHO cells (HPRT-) were stably cotransfected with pSV2-gpt and pi H3-CD2 vectors using the calcium phosphate coprecipitation technique. The effects of single low doses of ionizing radiation were studied in a CD2+ CHO clone. The CD2+ phenotype responsible for binding sheep erythrocytes and rosette formation, was not affected by X-rays doses in the range 2-6 cGy. However, after 10 cGy of X-irradiation, 50% of the cells lost the CD2+ phenotype. These results suggest that this CD2+ clone might be a very sensitive indicator of very low X-ray doses. The implications of the phenotypic changes, observed after very low doses of irradiation, are discussed.
Kitsiou
P
P
Institute of Biology, National Centre for Scientific Research Demokritos, Greece.
Sambani
C
C
Thomou
H
H
D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000528:radiation effects; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics* / Q000528:radiation effects; D000954:Antigens, Surface / Q000235:genetics* / Q000528:radiation effects*; D018801:CD2 Antigens; D016466:CHO Cells; D002999:Clone Cells / Q000528:radiation effects; D006224:Cricetinae; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase; D010641:Phenotype; D011829:Radiation Dosage; D011971:Receptors, Immunologic / Q000235:genetics* / Q000528:radiation effects; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D018801:CD2 Antigens; D011971:Receptors, Immunologic; D007041:Hypoxanthine Phosphoribosyltransferase