title: Necklace-like detachment of endothelial cell layer from arterial wall under low-calcium condition.
abstract: The diversified morphological manifestations in various tissues and organs obtained by administration of differing amounts of calcium chelating agents were reported in previous papers (Yamaguchi et al 1981 a & b; 1982; 1990; 1993). In our recent research described here, administration of a moderate dose of Na2EDTA over the short term demonstrated necklace-like detachment from the arterial wall without disruption of the endothelial cell chain. Intercellular spaces in the media just beneath the detached endothelial cell layer was stained strongly with colloidal iron staining. Electron microscopic observation revealed that the detached endothelial cells showed a lot of elongated anchoring villi from the basal surface, usually seen at the luminal surface, adhered to the degenerative and thin flattened internal elastic lamellae. The alteration of the colloidal iron staining of the vascular wall under the low-calcium condition is suggested to be induced by loosening of the molecular structure of glycosaminoglycans (GAGs) as well as glycoproteins (GPs), comprising the important component of the intercellular matrix and elastic lamellae, which would induce a change in their pasty or viscous character. This would be an accelerative factor for detachment of endothelial cells. Moreover, the lack of the waving of the internal elastic lamellae, trapping of endothelial cytoplasmic processes among them, would play the decisive role in the total detachment of the endothelial cell layer. On the other hand, the low-calcium condition did not adversely influence the joining of endothelial cells. The pathognomatic mechanism will be discussed, with a comparison made to the angiolytic changes provoked by a large amount of Na2EDTA.
authors.affiliation: Department of Pathology, School of Medicine, Keio University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Morisada
authors.forename: H
authors.initials: H
authors.lastname: Kaku
authors.forename: T
authors.initials: T
authors.lastname: Onodera
authors.forename: R
authors.initials: R
authors.lastname: Kurokawa
mesh_terms: D000818:Animals; D002118:Calcium / Q000172:deficiency*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000633:toxicity*; D004549:Elastin / Q000032:analysis; D004730:Endothelium, Vascular / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis; D006168:Guinea Pigs; D008297:Male; D008638:Mesenteric Arteries / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron
chemical_list: D006025:Glycosaminoglycans; D004549:Elastin; D004492:Edetic Acid; D002118:Calcium