title: New case of c-src gene transduction: the generation of virus PR2257.
abstract: PR2257 is a new replication-defective avian sarcoma virus which harbours in addition to the spliced version of the c-src gene also about 950 bp of no-coding cellular sequences located downstream from the c-src stop codon (Geryk et al., 1989). Comparison of the 950 bp region transduced by PR2257 with the chicken c-src cDNA (Dorai et al., 1991) and genomic sequences of the c-src 3' non-coding region from chicken and quail has shown that there are no additional introns. The c-src 3' non-coding region represents the largest c-src exon (No. 12) comprising about 2 kb. Absence of conserved open reading frames within this region in chicken and quail genomic DNAs excludes the possibility for coding a protein by these sequences. Also, the possibility was excluded that numerous endogenous virus-derived sequences identified in molecularly cloned PR2257 provirus played a role in the c-src transduction. After serial passaging of PR2257 virus in vivo a variant PR2257/16 was isolated. In PR2257/16, the size of the env gene was increased due to homologous recombination with a helper virus. In addition to mutations in the viral leader and the v-src coding region, a large deletion in transduced c-src 3' non-coding sequences was found in the PR2257/16 genome. The significance of genome modifications for selective advantage of this viral variant in vivo is discussed.
authors.affiliation: Unité de Recherche Associée 1443 du Centre National de la Recherche Scientifique, Institut Curie, Orsay, France.
authors.forename: P
authors.initials: P
authors.lastname: Dezelee
authors.forename: J V
authors.initials: JV
authors.lastname: Barnier
authors.forename: J
authors.initials: J
authors.lastname: Briest'anská
authors.forename: J
authors.initials: J
authors.lastname: Geryk
authors.forename: I
authors.initials: I
authors.lastname: Karakoz
authors.forename: A A
authors.initials: AA
authors.lastname: Michailik
authors.forename: J
authors.initials: J
authors.lastname: Nehyba
authors.forename: B A
authors.initials: BA
authors.lastname: Yatsula
authors.forename: A V
authors.initials: AV
authors.lastname: Rynditch
authors.forename: G
authors.initials: G
authors.lastname: Calothy
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D005814:Genes, Viral; D016391:Genes, src; D006801:Humans; D008969:Molecular Sequence Data; D014161:Transduction, Genetic