title: Dual activation of the cardiac Ca2+ channel alpha 1C-subunit and its modulation by the beta-subunit.
abstract: Ca2+ channels are heteromultimeric proteins in which the alpha 1-subunit forms the voltage-dependent Ca(2+)-selective ionic channel. We reported recently that coexpression of the beta-subunit with the cardiac alpha 1-subunit (alpha 1C) facilitates channel opening without affecting either the amplitude or the time course of the gating currents (13). Here we present evidence for the existence of two modes of channel opening. Xenopus oocytes expressing the alpha 1C-subunit alone display two modes of activation as indicated by the double-exponential time course of macroscopic ionic currents and the two open-time distributions of single channels. Coexpression of the beta-subunit potentiates Ca2+ currents by a relative increase of the fast-activating component, an acceleration of the slow component, and a larger proportion of long openings. We propose that multiple modes of gating are encoded in the alpha 1-subunit and that the beta-subunit increases Ca2+ channel opening by favoring a willing mode of gating in which the final transitions leading to channel opening are facilitated. In addition, we show that the carboxy terminus of alpha 1C also modulates the channel-gating behavior.
authors.affiliation: Department of Molecular Physiology, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: A
authors.initials: A
authors.lastname: Neely
authors.forename: R
authors.initials: R
authors.lastname: Olcese
authors.forename: P
authors.initials: P
authors.lastname: Baldelli
authors.forename: X
authors.initials: X
authors.lastname: Wei
authors.forename: L
authors.initials: L
authors.lastname: Birnbaumer
authors.forename: E
authors.initials: E
authors.lastname: Stefani
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000737:chemistry / Q000502:physiology*; D018118:Chloride Channels / Q000502:physiology; D004095:Dihydropyridines / Q000494:pharmacology; D004553:Electric Conductivity; D005260:Female; D015640:Ion Channel Gating / Q000502:physiology; D007700:Kinetics; D046911:Macromolecular Substances; D009206:Myocardium / Q000737:chemistry; D009543:Nifedipine / Q000494:pharmacology; D015737:Nisoldipine / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism; D013329:Structure-Activity Relationship; D014982:Xenopus laevis
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D018118:Chloride Channels; D004095:Dihydropyridines; D046911:Macromolecular Substances; D015737:Nisoldipine; C038806:1,4-dihydropyridine; D009543:Nifedipine