Immunohistochemical demonstration of expression of tyrosine hydroxylase in cerebellar Purkinje cells of the human and mouse.
We demonstrated the expression of tyrosine hydroxylase (TH) and phenylethanolamine-N-methyltransferase (PNMT) in cerebellar Purkinje cells of human and mouse brains by immunohistochemistry. At the age of 8 months, vermal Purkinje cells in the human cerebellum were immunoreactive with anti-TH or anti-human specific PNMT antiserum. We also found TH-positive Purkinje cells in the mouse brain. TH immunoreactivity first appeared in the Purkinje cells in the caudal vermis from postnatal day 8 (P8), and enlarged numbers between P13 and P15. After gradual reduction to P19, the TH-positive Purkinje cells again multiplied the number from the age of 4 weeks, and maintained in a large number up to old stage. At the age of 11 months, the other groups of TH-positive Purkinje cells appeared in the flocculus and paraflocculus of the mouse cerebellum.
Fujii
T
T
Department of Anatomy, School of Medicine, Fujita Health University, Aichi, Japan.
Sakai
M
M
Nagatsu
I
I
D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology / Q000254:growth & development*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D051379:Mice; D010625:Phenylethanolamine N-Methyltransferase / Q000276:immunology / Q000378:metabolism; D011689:Purkinje Cells / Q000201:enzymology*; D013045:Species Specificity; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology / Q000378:metabolism*
D014446:Tyrosine 3-Monooxygenase; D010625:Phenylethanolamine N-Methyltransferase