Detergent-induced reversible denaturation of the photosystem II reaction center: implications for pigment-protein interactions.
Incubation of the D1-D2-cytochrome b559 complex with Triton X-100 modified the protein secondary structure, caused significant spectral modifications, and reduced the formation of light-induced spin-polarized triplet electron paramagnetic resonance (EPR) signal. After 24 h of incubation, the absorption spectrum shifted from 675.5 to 671.5 nm and the fluorescence spectrum shifted from 682 to 672 nm. These shifts were accompanied by an increase in the chlorophyll fluorescence yield and by decreases in the intensity of the circular dichroism in the red region and the secondary electron transport activity. The intensity of the light-induced triplet EPR signal was also markedly reduced in the same experimental conditions. Substitution of dodecyl beta-maltoside for Triton X-100 reversed all the above-mentioned parameters to the values exhibited by the native D1-D2-Cyt b559 complex, including the characteristic triplet EPR signal. We concluded that all observed changes were due to the destruction of P680 with Triton X-100 and to the reestablishment of P680 in the presence of dodecyl beta-maltoside. The easier but certainly not the only possible explanation to all these phenomena is to consider a dimeric structure for P680, at least in its ground state, where interactions take place within the two dimeric chromophores and with the apoprotein. Such a dimeric structure would be very sensitive to small modifications of the P680 domain, which convert the dimer absorbing at 680 into two chlorophyll monomers absorbing near 670 nm. The dodecyl beta-maltoside reestablished the structure of the native P680 domain.
Montoya
G
G
Estación Experimental de Aula Dei, CSIC, Zaragoza, Spain.
Cases
R
R
Rodríguez
R
R
Aured
M
M
Picorel
R
R
D002338:Carotenoids / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D002942:Circular Dichroism; D003573:Cytochrome b Group / Q000737:chemistry / Q000187:drug effects; D004578:Electron Spin Resonance Spectroscopy; D045342:Light-Harvesting Protein Complexes; D017830:Octoxynol / Q000494:pharmacology*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000187:drug effects*; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000737:chemistry / Q000187:drug effects; D011489:Protein Denaturation; D017433:Protein Structure, Secondary / Q000187:drug effects*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D014364:Tryptophan / Q000737:chemistry; D014675:Vegetables / Q000737:chemistry
D003573:Cytochrome b Group; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; C070970:photosystem II, chlorophyll-binding protein, CP-47; D002734:Chlorophyll; D002338:Carotenoids; D014364:Tryptophan; D017830:Octoxynol; C019313:cytochrome b559