Correlation between [125I]calmodulin binding and lipid fluidity in synaptic plasma membranes: effects of ethanol and other short-chain alcohols.
Ethanol inhibits [125I]calmodulin binding to synaptic plasma membranes from rat brain, and this inhibition is correlated in a concentration-dependent manner with the increase of membrane fluidity, as determined by diphenylhexatriene fluorescence polarization. Moreover, several short-chain alcohols that increase membrane fluidity are also effective inhibitors of [125I]calmodulin binding. These data support the notion that ethanol inhibits calmodulin binding by increasing lipid fluidity of the synaptic membranes.
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Department of Pharmacology and Molecular Biology, Chicago Medical School, IL 60064-3095.
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D000818:Animals; D001665:Binding Sites; D002147:Calmodulin / Q000187:drug effects*; D000431:Ethanol / Q000494:pharmacology*; D050356:Lipid Metabolism; D008297:Male; D008560:Membrane Fluidity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013570:Synaptic Membranes / Q000187:drug effects*
D002147:Calmodulin; D000431:Ethanol