title: Delayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model.
abstract: A mouse model incorporating the epitheliotropic nature of human papillomavirus (HPV) infections has been used to study an immune response to HPV type 16 (HPV-16) E6 protein in vivo. Using a transplantation technique, a novel immortal keratinocyte cell line expressing the E6 protein has been grafted onto syngeneic mice to re-form a differentiated epithelium overlying a granulation tissue bed. By this approach the presentation of viral antigens to the immune system can be modelled in a way analogous to the natural infection. Here we report a delayed-type hypersensitivity (DTH) reaction in grafted mice challenged intradermally with a recombinant vaccinia virus expressing the HPV-16 E6 protein. The specificity of the response was confirmed by the absence of a DTH reaction to challenge with virus expressing either HPV-16 E7 or L1 protein.
authors.affiliation: Department of Pathology, University of Cambridge, U.K.
authors.forename: M A
authors.initials: MA
authors.lastname: Chambers
authors.forename: S N
authors.initials: SN
authors.lastname: Stacey
authors.forename: J R
authors.initials: JR
authors.lastname: Arrand
authors.forename: M A
authors.initials: MA
authors.lastname: Stanley
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D015603:Keratinocytes / Q000637:transplantation / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D012097:Repressor Proteins; D012680:Sensitivity and Specificity; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D000956:Antigens, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins; D012097:Repressor Proteins