Molecular cloning of the human homeobox gene goosecoid (GSC) and mapping of the gene to human chromosome 14q32.1.
Goosecoid is a homeobox gene first isolated from a Xenopus dorsal lip cDNA library. Homologous genes have been isolated from mouse, zebrafish, and chick. In all species examined, the gene is expressed and plays an important role during the process of gastrulation in early embryonic development. We report here the cloning of the human goosecoid gene (GSC) from a genomic library and the sequence of its encoded protein. The genomic organization and protein sequence of the human gene are highly conserved with respect to those of its Xenopus and mouse counterparts: all three genes consist of three exons, with conserved exon-intron boundaries; the sequence of the homeodomain is 100% conserved in most vertebrates. Using somatic cell hybrid and chromosomal in situ hybridization, the gene was mapped to chromosome 14q32.1.
Blum
M
M
Molecular Biology Institute, University of California at Los Angeles 90024-1737.
De Robertis
E M
EM
Kojis
T
T
Heinzmann
C
C
Klisak
I
I
Geissert
D
D
Sparkes
R S
RS
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002645:Chickens / Q000235:genetics; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D005091:Exons; D005801:Genes, Homeobox; D015698:Genomic Library; D051956:Goosecoid Protein; D018398:Homeodomain Proteins; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D007438:Introns; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D012097:Repressor Proteins; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014981:Xenopus / Q000235:genetics; D015027:Zebrafish / Q000235:genetics
D004268:DNA-Binding Proteins; C497992:GSC protein, human; D051956:Goosecoid Protein; C497993:Gsc protein, mouse; D018398:Homeodomain Proteins; D012097:Repressor Proteins; D014157:Transcription Factors