Use of firefly luciferase gene for plasmid copy number determination.
A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimate plasmid copy number by densitometry is also described.
Coronado
C
C
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Spain.
Vázquez
M E
ME
Cebolla
A
A
Palomares
A J
AJ
D000818:Animals; D001517:Coleoptera / Q000201:enzymology / Q000235:genetics*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005784:Gene Amplification; D015870:Gene Expression; D017344:Genes, Insect; D017930:Genes, Reporter; D005819:Genetic Markers; D005821:Genetic Techniques; D005822:Genetic Vectors; D008156:Luciferases / Q000235:genetics*; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*
D004251:DNA Transposable Elements; D005819:Genetic Markers; D008156:Luciferases