c-myc protein expression during cell cycle phases in differentiating HL-60 cells.
We examined c-myc protein expression in cell cycle phases during differentiation induction of HL-60 cells by flow cytometry using an indirect immunofluorescence method. In exponentially proliferating HL-60 cells, c-myc protein was expressed in a cell cycle dependent manner. During the differentiation induction of HL-60 cells with dimethylsulfoxide (DMSO), c-myc protein was rapidly down-regulated in the G1/0 specific phase prior to the appearance of differentiation associated markers. Our results indicate that c-myc protein functions in the G1/0 specific phase in cellular differentiation, and the rapid down-regulation of c-myc protein in G1/0 phase is closely associated with initial differentiation programs.
Kumakura
S
S
Third Division of Internal Medicine, Shimane Medical University, Izumo, Japan.
Ishikura
H
H
Tsumura
H
H
Hayashi
H
H
Endo
J
J
Tsunematsu
T
T
D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000187:drug effects
D009363:Neoplasm Proteins; D016271:Proto-Oncogene Proteins c-myc; D004121:Dimethyl Sulfoxide