Linkage between the APOB gene and serum ApoB levels in a large pedigree from the Bogalusa Heart Study.
Maximum likelihood linkage analyses were performed to test for linkage between serum apoB levels and several candidate gene markers including apolipoprotein B, lipoprotein lipase, hepatic lipase, cholesterol ester transfer protein, and apolipoprotein AI in a large pedigree. Parameters of general Mendelian inheritance derived from maximum likelihood segregation analysis of the serum apoB levels were used in the linkage analysis. The highest two-point lod score between the quantitative trait and a marker defined by a single restriction digest was 1.86 at recombination fraction (theta) = 0. This was observed for linkage between serum apoB levels and the presence or absence of a PvuII digestion site in the apoB gene. Linkage between serum apoB levels and polymorphisms of the apoB gene defined by the two restriction digests EcoR1 and PvuII was supported by a lod score of 3.30, while inclusion of VNTR typings led to a lod score of 2.33. None of the other candidate genes gave positive evidence of linkage.
Laing
A E
AE
Biostatistics Division, Howard University Cancer Center, Washington, D.C.
Amos
C I
CI
DeMeester
C
C
Diep
A
A
Xia
Y R
YR
Elston
R C
RC
Srinivasan
S R
SR
Berenson
G S
GS
Lusis
A J
AJ
D000293:Adolescent; D016632:Apolipoprotein A-I / Q000032:analysis / Q000235:genetics; D001053:Apolipoproteins / Q000032:analysis / Q000235:genetics; D001055:Apolipoproteins B / Q000032:analysis* / Q000235:genetics*; D002352:Carrier Proteins / Q000097:blood / Q000235:genetics; D053480:Cholesterol Ester Transfer Proteins; D002874:Chromosome Mapping; D003327:Coronary Disease / Q000097:blood* / Q000453:epidemiology / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006023:Glycoproteins; D006239:Haplotypes; D006579:Heterozygote; D006801:Humans; D016013:Likelihood Functions; D008071:Lipoprotein Lipase / Q000097:blood / Q000235:genetics; D008126:Lod Score; D008145:Louisiana / Q000453:epidemiology; D008957:Models, Genetic; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics
D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D001055:Apolipoproteins B; C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D005819:Genetic Markers; D006023:Glycoproteins; C020098:DNA modification methylase EcoRI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D008071:Lipoprotein Lipase; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific