The polymorphic ADP-ribosyltransferase (NAD+) pseudogene 1 in humans interrupts an endogenous pol-like element on 13q34.
A recently constructed chromosome 13-enriched library was used to isolate the entire human ADP-ribosyltransferase (NAD+) pseudogene 1 gene (ADPRTP1) on 13q34. Recently, ADPRTP1 was shown to detect a restriction fragment length polymorphism that was associated with a predisposition to cancer. The complete sequence of the ADPRTP1 (3808 bases) was determined and found to resemble an intronless cDNA, including 137 bases of the 5' untranslated region and a short poly(A) tail. A previously uncharacterized, endogenous pol-like element (1.53 kb) in which the DNA sequences were interrupted by the polymorphic ADPRTP1 was identified. The solitary pol-related retroviral element may represent a multimember family of viral-like DNA sequences dispersed throughout the human genome. Altogether a 9.25-kb genomic region that also encompassed two Alu elements and the long-terminal repeat-like element of the "O"-LTR family was characterized.
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Department of Biochemistry and Molecular Biology, Georgetown University School of Medicine, Washington, DC.
Deaven
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Istock
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Smulson
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D000595:Amino Acid Sequence; D015139:Blotting, Southern; D002882:Chromosomes, Human, Pair 13; D015751:Genes, pol; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011544:Pseudogenes; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D011065:Poly(ADP-ribose) Polymerases