Induction of Fos-like immunoreactivity in the rat hypothalamus by an endogenous feeding suppressant (2-buten-4-olide).
Central influence of 2-buten-4-olide (2-B4O), an endogenous feeding suppressant, was studied through immunohistochemical detection of Fos expression in the rat hypothalamus. Animals received 2-B4O (100 or 200 mg/kg i.p.) or saline 2 h before perfusion with 4% phosphate-buffered paraformaldehyde. The hypothalamus was removed and cut into vibratome sections. Immunohistochemical analysis revealed that injection of 2-B4O induced Fos-like immunoreactivity significantly in the paraventricular, dorsomedial and arcuate nuclei and the periventricular and lateral hypothalamic areas. These hypothalamic regions may control feeding under the influences of endogenous 2-B4O.
Hisano
S
S
Department of Anatomy, School of Medicine, University of Tokushima, Japan.
Uemura
N
N
Fukui
Y
Y
Miki
M
M
Zhang
R
R
D015107:4-Butyrolactone / Q000031:analogs & derivatives; D000818:Animals; D004435:Eating / Q000502:physiology*; D005663:Furans / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D005663:Furans; D016760:Proto-Oncogene Proteins c-fos; C004511:butenolide; D015107:4-Butyrolactone