[The effect of L-glutamic acid on the ADP-induced aggregation and Ca, Mg-ATpase activity of human thrombocytes].
In the study of L-glutaminic acid effects on human platelet functional activity it was established that 30-60-min treatment of platelet-rich plasma with glutamate in concentration 0.1-1.0 mM at 37 degrees C results in a marked platelet activation. The latter noticeably enhances ADP-induced aggregation persistent against antiaggregation adenosine concentrations. With increasing duration of the incubation a glutaminic acid minimal effective concentration lowers. Proaggregatory and anti-adenosine effects of L-glutaminic acid are inhibited by kainic acid. The experiments evidence that 30-min preincubation of platelet membranes with 1 mM L-glutaminic acid produces inhibition of cAMP-induced activation of Ca, Mg-ATPase returning the activity of the enzyme to baseline levels. No glutamate-related changes in calcium pump of platelets are reported.
Gurevich
V S
VS
Mikhaĭlova
I A
IA
Popov
Iu G
IuG
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D001782:Blood Donors; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000097:blood / Q000187:drug effects*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D006801:Humans; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects*; D013997:Time Factors
D005971:Glutamates; D018698:Glutamic Acid; D000244:Adenosine Diphosphate; D017301:Ca(2+) Mg(2+)-ATPase
Vliianie L-glutaminovoĭ kisloty na ADF-indutsirovannuiu agregatsiiu i aktivnost' Ca, Mg-ATFazy trombotsitov cheloveka.