Developmental profile of P element transposition in Drosophila somatic cells.
Transposition of the P element during Drosophila ontogenesis was monitored. A modified P element was transposed by the P delta 2-3 transposase source. P elements inserted into the genome were cloned by the plasmid rescue at various developmental stages of the G1 hybrid to trace events in somatic cells. The transposed elements were directly counted by analyzing RFLP of genomic DNA fragments flanking the P elements. Transposition began from the late embryonic stage, but occurred rarely. Frequent transposition was observed from the late third instar to early pupal stage. From these results, transposition of the P element would appear to be affected by the developmental state of somatic host cells.
Togashi
S
S
Laboratory of Cell Biology, Mitsubishi Kasai Institute of Life Sciences, Tokyo, Japan.
Ueda
R
R
Takahisa
M
M
Kondo
K
K
Miyake
T
T
D000818:Animals; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D005260:Female; D005786:Gene Expression Regulation; D007814:Larva; D008297:Male; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D011679:Pupa; D019895:Transposases
D004251:DNA Transposable Elements; D009713:Nucleotidyltransferases; D019895:Transposases