Transformation of Chlorobium limicola by a plasmid that confers the ability to utilize thiosulfate.
A modified transformation method for Chlorobium limicola confirms the role of an endogenous plasmid in thiosulfate metabolism. The plasmid was present in two forma specialis thiosulfatophilum (Tio+) strains and absent in one non-thiosulfate-utilizing (Tio-) strain. The plasmid (size, 14 kb) was transferred by transformation from Tio- to Tio+. The chromosomal restriction patterns, analyzed by pulsed-field gel electrophoresis, were used to distinguish between the different strains.
Méndez-Alvarez
S
S
Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Pavón
V
V
Esteve
I
I
Guerrero
R
R
Gaju
N
N
D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D010957:Plasmids / Q000235:genetics*; D013885:Thiosulfates / Q000378:metabolism*; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D013885:Thiosulfates; D015252:Deoxyribonucleases, Type II Site-Specific