title: Robotic sample preparation and high-performance liquid chromatographic analysis of verlukast in human plasma.
abstract: A fully automated HPLC assay has been developed and validated for the quantitation of verlukast, a leukotriene D4 antagonist, in human plasma. An upgraded Zymate I robotic system was utilized to perform protein precipitation and on-line injection followed by reversed-phase HPLC with fluorescence detection. Inter-day accuracy and precision were 100.8 and 4.6%, respectively, for the low quality control standards (0.125 microgram/ml). The automated robotic method was shown to be more efficient and accurate than the manual method.
authors.affiliation: Department of Drug Metabolism, Merck Research Laboratories, West Point, PA 19486.
authors.forename: J Y
authors.initials: JY
authors.lastname: Hsieh
authors.forename: C
authors.initials: C
authors.lastname: Lin
authors.forename: B K
authors.initials: BK
authors.lastname: Matuszewski
mesh_terms: D001993:Bronchodilator Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D011422:Propionates / Q000097:blood*; D011786:Quality Control; D011804:Quinolines / Q000097:blood*; D015203:Reproducibility of Results; D012371:Robotics; D013048:Specimen Handling; D013050:Spectrometry, Fluorescence
chemical_list: D001993:Bronchodilator Agents; D011422:Propionates; D011804:Quinolines; C059141:verlukast