The yeast two-hybrid system for studying protein-protein interactions.
A great number of investigators are currently employing the yeast two-hybrid system to study protein-protein interactions. Recent applications and newer configurations of the technique include yeast strains with improved selectivity and screens for false-positive clones.
Luban
J
J
Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
Goff
S P
SP
D000818:Animals; D001709:Biotechnology / Q000379:methods; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005189:False Positive Reactions; D005656:Fungal Proteins / Q000378:metabolism*; D005821:Genetic Techniques; D008322:Mammals; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D005656:Fungal Proteins; D011506:Proteins; D014157:Transcription Factors; D004247:DNA; D012321:DNA-Directed RNA Polymerases