title: Fate of allogeneic or syngeneic cells in intravenous or portal vein injection: possible explanation for the mechanism of tolerance induction by portal vein injection.
abstract: In this report we examine the fate of donor cells injected via different routes. When PKH-26-labeled C57BL/6 (B6) spleen cells were intravenously (i.v.) injected into BALB/c mice, the donor cells were rejected within 3 days. In contrast, when the same B6 spleen cells were portal venously (p.v.) injected, they were trapped in the recipient liver. When allogeneic or syngeneic whole bone marrow cells (BMC) or cells in a hemopoietic stem cell (HSC)-enriched fraction were either i.v. or p.v. injected, the cells accumulated in the liver. The cells trapped in the liver were found to be wheat germ agglutinin (WGA)-positive HSC. When B6 thymocytes were p.v. or i.v. injected into BALB/c mice, they were rapidly rejected. When BALB/c mice were i.v. preimmunized with unlabeled B6 spleen cells, BMC or thymocytes, the p.v. or i.v. injected PKH-26-labeled B6 spleen cells were rejected rapidly (within 2 days). In contrast, when BALB/c mice were p.v. preimmunized with B6 spleen cells or BMC, the p.v. or i.v. injected PKH-26-labeled B6 spleen cells were not rejected. The cells responsible for the tolerance induction were found to be HSC trapped in the liver. Delayed-type hypersensitivity assays revealed that the tolerance could be maintained for more than 49 days by p.v. injection plus i.v. injection (at intervals of 2 weeks) of HSC. These findings indicate that HSC trapped in the liver play a crucial role in the induction and maintenance of p.v. tolerance.
authors.affiliation: First Department of Pathology, Kansai Medical University, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: R
authors.initials: R
authors.lastname: Yasumizu
authors.forename: K
authors.initials: K
authors.lastname: Sugiura
authors.forename: F
authors.initials: F
authors.lastname: Hashimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Amoh
authors.forename: Z
authors.initials: Z
authors.lastname: Lian
authors.lastname: Cherry
authors.forename: N
authors.initials: N
authors.lastname: Nishio
authors.forename: S
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002465:Cell Movement / Q000276:immunology; D005260:Female; D005456:Fluorescent Dyes; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006968:Hypersensitivity, Delayed; D007108:Immune Tolerance / Q000276:immunology*; D007114:Immunization / Q000379:methods; D007275:Injections, Intravenous / Q000379:methods; D008099:Liver / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009930:Organic Chemicals; D011169:Portal Vein / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D014184:Transplantation, Homologous / Q000276:immunology*; D014185:Transplantation, Isogeneic / Q000276:immunology
chemical_list: D005456:Fluorescent Dyes; D009930:Organic Chemicals; C070080:PKH 26