Brefeldin A affects West Nile virus replication in Vero cells but not C6/36 cells.
A fungal metabolite brefeldin A (BFA) was used to study virus-host interaction in glycoprotein processing in West Nile virus-infected Vero and C6/36 cells. The results indicated that as little as 1 microgram/ml of BFA resulted in complete breakdown in the Golgi organelle in infected Vero cells. This led to modifications of the glycoproteins which could not be efficiently used in infectious virion formation. In contrast, as much as 10 micrograms/ml of BFA in culture medium did not affect either glycoprotein formation or production of infectious particles in C6/36 cells. The results showed that in Vero cells, the transport of glycoproteins to the Golgi apparatus is important in West Nile virus infection. It also showed that BFA could be used as a tool to understand further the trafficking of glycoprotein from the ER to Golgi in flavivirus infection in Vero cells.
Sreenivasan
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Department of Microbiology, National University of Singapore, Kent Ridge, Singapore.
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D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D020126:Brefeldin A; D002460:Cell Line; D009033:Culicidae; D003517:Cyclopentanes / Q000494:pharmacology*; D006023:Glycoproteins / Q000187:drug effects / Q000378:metabolism; D014709:Vero Cells; D014764:Viral Proteins / Q000187:drug effects* / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects* / Q000502:physiology; D014902:West Nile virus / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D000998:Antiviral Agents; D003517:Cyclopentanes; D006023:Glycoproteins; D014764:Viral Proteins; D020126:Brefeldin A