title: Expression of the p53 and Wilms' tumor suppressor genes in the rat ovary: gonadotropin repression in vivo and immunohistochemical localization of nuclear p53 protein to apoptotic granulosa cells of atretic follicles.
abstract: We have previously demonstrated that the gonadotropin-mediated inhibition of apoptosis in ovarian granulosa cells is linked to changes in the expression of several cell death-related genes, including members of the bcl-2 gene family (bcl-2, bax, and bcl-x). Recently, the product of the p53 tumor suppressor gene, a protein reported to play a critical role in regulating cell proliferation and death, has been shown to directly modulate the transcriptional activity of the bcl-2 and bax genes. In addition, the actions of p53 may be amplified through a cooperative interaction with another tumor suppressor protein, the product of the Wilms' tumor suppressor gene (WT-1). Based on our identification of a potential role for bcl-2-related factors in regulating granulosa cell apoptosis and the reported function of p53 as a regulator of bcl-2 and bax gene transcription in extragonadal cells, the present studies were conducted to determine whether the p53 and WT-1 genes are expressed and gonadotropin regulated in the rat ovary and to investigate whether granulosa cell apoptosis is linked to elevated levels of tumor suppressor gene expression. Northern blot analysis of total RNA prepared from immature (27-day-old) rat ovaries revealed the presence of a single p53 messenger RNA (mRNA) transcript (2.0 kilobases) and multiple WT-1 messages (1.8, 3.5, and 7.5 kilobases). Subcutaneous injection of immature rats with 10 IU equine CG (eCG) reduced the levels of p53 and WT-1 mRNA to 71 +/- 9% (P < 0.05) and 46 +/- 3% (P < 0.05), respectively, of saline-treated control levels after 2 days. The inhibition of tumor suppressor gene expression by eCG treatment was associated with a marked reduction in the number of apoptotic granulosa cells and atretic follicles. Furthermore, immunohistochemical analysis revealed that p53 protein was localized exclusively to nuclei of apoptotic granulosa cells of atretic follicles, and that p53 immunostaining was reduced to undetectable levels after in vivo treatment with eCG. To further evaluate whether granulosa cell apoptosis is linked to increased expression of tumor suppressor genes, we analyzed levels of p53 and WT-1 mRNA in antral follicles induced to undergo atresia in vitro by serum-free culture.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Population Dynamics, Johns Hopkins University, Baltimore, Maryland 21205-2179.
authors.forename: K I
authors.initials: KI
authors.lastname: Tilly
authors.forename: S
authors.initials: S
authors.lastname: Banerjee
authors.forename: P P
authors.initials: PP
authors.lastname: Banerjee
authors.forename: J L
authors.initials: JL
authors.lastname: Tilly
mesh_terms: D000818:Animals; D017209:Apoptosis; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000737:chemistry; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005496:Follicular Atresia; D015870:Gene Expression; D016162:Genes, Wilms Tumor; D016158:Genes, p53; D006107:Granulosa Cells / Q000737:chemistry*; D007150:Immunohistochemistry; D009924:Organ Culture Techniques; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D006063:Chorionic Gonadotropin; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53