Antibodies to HMG proteins in patients with drug-induced autoimmunity.
OBJECTIVE
To determine the prevalence of autoantibodies to high-mobility group (HMG) proteins in sera from patients with drug-induced lupus (DIL).


METHODS
Forty-two patients who developed autoantibodies and/or lupus after treatment with procainamide or other drugs were tested for HMG autoantibodies by immunoblotting and enzyme-linked immunosorbent assay (ELISA).


RESULTS
Twenty-eight of the 42 sera (67%) bound HMG-14 and/or HMG-17. In comparison, 9 of 42 (21%) bound HMG-1 and/or HMG-2. There was a good correlation between ELISA results and binding on immunoblots.


CONCLUSIONS
The high prevalence of antibodies to the nucleosomal core HMGs (HMG-14 and HMG-17) in DIL patients adds evidence implicating nucleosomes as immunogens in drug-induced autoimmunity.
Ayer
L M
LM
Department of Medicine, University of Calgary, Alberta, Canada.
Rubin
R L
RL
Dixon
G H
GH
Fritzler
M J
MJ
D000070:Acebutolol / Q000627:therapeutic use; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000097:blood / Q000139:chemically induced; D015551:Autoimmunity / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006609:High Mobility Group Proteins / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008177:Lupus Vulgaris / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy; D011342:Procainamide / Q000009:adverse effects; D012460:Sulfasalazine / Q000627:therapeutic use
D001323:Autoantibodies; D006609:High Mobility Group Proteins; D012460:Sulfasalazine; D000070:Acebutolol; D011342:Procainamide