Identification and cloning of human chaperonin 10 homologue.
We have identified a heat-shock-inducible 10 kDa protein in the human hepatoma cell line HepG2. The total RNA extracted from the heat-shocked cells was amplified by reverse transcription PCR (polymerase chain reaction) using 21 5' and 18 3' oligonucleotides of rat cpn10 (chaperonin10) cDNA as primers. Sequencing of the above PCR fragment showed a very high homology between human, bovine and rat cpn10 cDNA. The predicted amino acid sequence revealed a 100% identity with the bovine homologue.
Monzini
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Italfarmaco S.p.A., Centro Ricerche, Milano, Italy.
Legname
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Marcucci
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F
Gromo
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G
Modena
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D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002417:Cattle / Q000235:genetics; D018835:Chaperonin 10; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D018835:Chaperonin 10; D017931:DNA Primers; D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D012333:RNA, Messenger