Effect of fat feeding on human intestinal apolipoprotein B mRNA levels and editing.
Our purpose was to assess the effect of a fat-rich meal on intestinal apolipoprotein B (apoB) mRNA levels and editing. We obtained jejunal biopsies from eight healthy adults in the fasting state and 3 h after a meal containing 1 g/kg of fat. In the fasting state, 93% of the apoB mRNA contained the editing sequence resulting in apoB-48 production. Feeding induced no significant changes in apoB mRNA levels or editing. Our data are consistent with the concept that the significant increase in apoB-48 within triglyceride-rich lipoproteins in postprandial plasma is not due to alterations in apoB gene expression or editing.
Lopez-Miranda
J
J
Lipid Metabolism Laboratory, Tufts University, Boston, MA 02111.
Kam
N
N
Osada
J
J
Rodriguez
C
C
Fernandez
P
P
Contois
J
J
Schaefer
E J
EJ
Ordovas
J M
JM
D001055:Apolipoproteins B / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D004041:Dietary Fats / Q000494:pharmacology*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008969:Molecular Sequence Data; D017393:RNA Editing; D012333:RNA, Messenger / Q000032:analysis*
D001055:Apolipoproteins B; D004041:Dietary Fats; D008055:Lipids; D012333:RNA, Messenger