Selegiline enhances survival and neurite outgrowth of MPP(+)-treated dopaminergic neurons.
Selegiline was added to co-cultures of mesencephalon and neostriatum of C57BL/6 mouse embryos according to three schemes: (i) before and (ii) after cells were exposed to the toxin 1-methyl-4-phenylpyridinium (MPP+), (iii) in control cultures. In all schemes, selegiline enhanced the morphological differentiation of dopaminergic neurons and with delayed treatment, significantly increased their survival. These results indicate that selegiline exhibits trophic-like actions and can rescue MPP(+)-injured dopaminergic neurons in cultures.
Koutsilieri
E
E
Institute of Medical Chemistry, Veterinary University of Vienna, Austria.
O'Callaghan
J F
JF
Chen
T S
TS
Riederer
P
P
Rausch
W D
WD
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity; D000704:Analysis of Variance; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D051379:Mice; D008810:Mice, Inbred C57BL; D017072:Neostriatum / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D016501:Neurites / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D012642:Selegiline / Q000494:pharmacology*
D012642:Selegiline; D015655:1-Methyl-4-phenylpyridinium