title: Determination of a complete set of coupling constants in 13C-labeled oligonucleotides.
abstract: Three experiments are introduced to determine a complete set of coupling constants in RNA oligomers. In the HCCH-E.COSY experiment, the vicinal proton-proton coupling constants can be measured with high accuracy. In the P-FIDS-CT-HSQC experiment, vicinal proton-phosphorus and carbon-phosphorus couplings are measured that depend on the phosphodiester backbone torsion angles beta and epsilon. In the refocussed HMBC experiment, vicinal carbon-proton couplings are measured that depend on the glycosidic torsion angle chi.
authors.affiliation: Institut für Organische Chemie, Universität Frankfurt, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schwalbe
authors.forename: J P
authors.initials: JP
authors.lastname: Marino
authors.forename: G C
authors.initials: GC
authors.lastname: King
authors.forename: R
authors.initials: R
authors.lastname: Wechselberger
authors.forename: W
authors.initials: W
authors.lastname: Bermel
authors.forename: C
authors.initials: C
authors.lastname: Griesinger
mesh_terms: D001483:Base Sequence; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D009843:Oligoribonucleotides