title: Differences in antigen expression within and between 10 isolates of Giardia duodenalis.
abstract: In this study indirect immunofluorescence was performed on both live and fixed trophozoites to determine the level of variability in surface antigen expression between 14 Giardia duodenalis isolates, using a monoclonal antibody raised against the Portland 1 isolate (ATCC No. 30888). Subsets of antigen positive cells were detected in 13 isolates ranging in number from < 1% to 50% of the total population. The differences in antigen expression between 10 uncloned isolates did not correlate with genetic differences determined using isoenzyme analysis. Examination of four clones of the Portland 1 isolate showed that all of the progeny expressed surface antigen at significantly different levels to the parent.
authors.affiliation: Institute for Molecular Genetics and Animal Disease, Murdoch University, Australia.
authors.forename: R M
authors.initials: RM
authors.lastname: Hopkins
authors.forename: R C
authors.initials: RC
authors.lastname: Thompson
authors.forename: R P
authors.initials: RP
authors.lastname: Hobbs
authors.forename: A J
authors.initials: AJ
authors.lastname: Lymbery
authors.forename: N
authors.initials: N
authors.lastname: Villa
authors.forename: T M
authors.initials: TM
authors.lastname: Smithyman
mesh_terms: D000818:Animals; D000940:Antigenic Variation / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis*; D002415:Cats; D016829:Giardia lamblia / Q000235:genetics / Q000276:immunology*; D006801:Humans; D013045:Species Specificity
chemical_list: D000953:Antigens, Protozoan