title: Effects of luteinizing hormone and follicle-stimulating hormone on the progesterone receptor induction in chicken granulosa cells in vivo.
abstract: Progesterone receptor (PR) is increased in the granulosa cells prior to ovulation. In order to investigate the factors that induce PR in granulosa cells, we examined whether the PR is induced in granulosa cells by the injection of chickens with ovine luteinizing hormone (oLH) or porcine follicle-stimulating hormone (pFSH) by Western blot analysis. In the oLH-stimulated birds, PR of 79 kDa and 110 kDa were recognized in the granulosa cells of the largest follicle, whereas a PR of 110 kDa was observed in those of the smaller follicles (the second and third largest follicles). No immunoreaction product was observed in the granulosa cells of both the largest and the smaller follicles in pFSH-stimulated birds. In the control birds that were injected with saline, no immunoprecipitate was recognized in the granulosa cells of the largest or smaller follicles. These results suggest that LH may be involved in the induction of PR in the granulosa cells prior to ovulation.
authors.affiliation: Graduate School for International Development and Cooperation, Faculty of Applied Biological Science, Hiroshima University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: T
authors.initials: T
authors.lastname: Okamoto
authors.forename: T
authors.initials: T
authors.lastname: Tamura
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology*; D006107:Granulosa Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000502:physiology*; D011980:Receptors, Progesterone / Q000096:biosynthesis*
chemical_list: D011980:Receptors, Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone