Recombinant CD40 ligand exerts potent biologic effects on T cells.
Murine and human CD40 ligand (CD40L) were recently cloned, expressed, and shown to possess potent activity on human and murine B cells, including stimulation of proliferation and Ig secretion in the presence of cytokines. In addition to its action on B lymphocytes, this report demonstrates that CD40L induced both CD4+ and CD8+ T cells isolated from murine lymphoid tissues to proliferate in the presence of submitogenic dosages of Con A, PHA, CD3 mAb, and TCR-alpha beta mAb. The presence of CD40L during suboptimal TCR stimulation resulted in increased expression of the activation Ags IL-2R alpha and CD69 and increased IL-2 production. Taken together, these results show that CD40L is a potent activator of murine T cells and suggest that CD40L is involved in the regulation of T cell function mediated through T:T cell interaction.
Fanslow
W C
WC
Immunex Research and Development Corporation, Seattle, WA 98101.
Clifford
K N
KN
Seaman
M
M
Alderson
M R
MR
Spriggs
M K
MK
Armitage
R J
RJ
Ramsdell
F
F
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D023201:CD40 Ligand; D016827:CD8 Antigens / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007378:Interleukins / Q000494:pharmacology; D008024:Ligands; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000008:administration & dosage; D011247:Pregnancy; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000037:antagonists & inhibitors; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D016827:CD8 Antigens; D016207:Cytokines; D007378:Interleukins; D008024:Ligands; D008562:Membrane Glycoproteins; D008934:Mitogens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011994:Recombinant Proteins; D023201:CD40 Ligand