title: An African swine fever virus gene with similarity to the T-lymphocyte surface antigen CD2 mediates hemadsorption.
abstract: An open reading frame, LMW8-DR, in the African swine fever virus (ASFV) genome possesses striking similarity to the lymphocyte membrane antigen CD2. All characterized CD2 domains, including the amino-terminal signal sequence, IgV, hinge, IgC2, stalk, transmembrane, and proline-rich carboxy cytoplasmic domains, are highly conserved in the ASFV gene. Critical residues for the binding of the lymphocyte function-associated antigen (LFA-3) and CD59 and for T-cell activation are also partially conserved. LMW8-DR is actively transcribed in ASFV-infected swine macrophages and Vero cells at late times in the infection cycle and Vero and COS cells transiently expressing the LMW8-DR open reading frame hemadsorbed swine red blood cells. The structural and functional similarities of LMW8-DR to CD2, a protein that is involved in cell-cell adhesion and immune response modulation, suggest a possible role in the pathogenesis of ASFV infection.
authors.affiliation: Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, New York 11944.
authors.forename: M V
authors.initials: MV
authors.lastname: Borca
authors.forename: G F
authors.initials: GF
authors.lastname: Kutish
authors.forename: C L
authors.initials: CL
authors.lastname: Afonso
authors.forename: P
authors.initials: P
authors.lastname: Irusta
authors.forename: C
authors.initials: C
authors.lastname: Carrillo
authors.forename: A
authors.initials: A
authors.lastname: Brun
authors.forename: M
authors.initials: M
authors.lastname: Sussman
authors.forename: D L
authors.initials: DL
authors.lastname: Rock
mesh_terms: D000358:African Swine Fever Virus / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry*; D001483:Base Sequence; D018801:CD2 Antigens; D002478:Cells, Cultured; D017124:Conserved Sequence / Q000235:genetics; D005814:Genes, Viral / Q000502:physiology*; D006382:Hemadsorption / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011971:Receptors, Immunologic / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; C086096:LMW8-DR protein, African swine fever virus; D011971:Receptors, Immunologic; D014764:Viral Proteins