c-Ha-ras BamHI RFLP in human urothelial tumors and point mutations in hot codons.
High-molecular-weight DNAs from 30 bladder and renal cell carcinomas (RCC) were isolated and the c-Ha-ras gene BamHI RFLP was examined. Amplification of c-Ha-ras with normal localization with regard to the size of alleles was found only in one case. One of the normally localized c-Ha-ras allele termed RCC c-Ha-ras of a length of about 6.6 kbp was cloned and an oncogene-activating point mutation was identified using two restriction enzymes. After comparison of CfrI and Cfr10I cleavage maps of RCC c-Ha-ras to complete nucleotide sequences of EJ/T24 c-Ha-ras oncogene and its normal counterpart, a point mutation was identified within codon 11 or 12. The use of CfrI and Cfr10I is of value for clinical practice in identification of point mutations in c-Ha-ras PCR product in neoplasia accompanied by somatic mutation of c-Ha-ras. The correlation among c-Ha-ras allele, amplification/loss, presence of point mutation and progression of neoplasia is discussed.
Weismanová
E
E
Slovak Academy of Sciences, Department of Experimental Tumor Biology Bratislava.
Kalúz
S
S
Skorvaga
M
M
D000483:Alleles; D001483:Base Sequence; D015139:Blotting, Southern; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics*; D004273:DNA, Neoplasm / Q000302:isolation & purification; D015245:Deoxyribonuclease BamHI; D004926:Escherichia coli; D011905:Genes, ras; D015698:Genomic Library; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
D003062:Codon; D004273:DNA, Neoplasm; D015245:Deoxyribonuclease BamHI