title: Nicotine increases diazepam binding inhibitor (DBI) mRNA in primary cultured neurons.
abstract: The effect of nicotine on the expression of diazepam binding inhibitor (DBI) mRNA in primary cultured cerebral cortical neurons was examined using Northern blot analysis. Nicotine exposure (0.001-10 microM) for 24 h increased the DBI mRNA level in a dose-dependent manner, whereas the beta-actin mRNA level showed no change. This effect of nicotine was faded out over 48 h of its exposure. Hexamethonium (100 microM) completely abolished the nicotine-induced increase in DBI mRNA expression. These results indicate that nicotine increases the expression of DBI mRNA in cerebral cortical neurons via the activation of nicotinic acetylcholine receptor.
authors.affiliation: Department of Pharmacology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Katsura
authors.forename: S
authors.initials: S
authors.lastname: Ohkuma
authors.forename: D T
authors.initials: DT
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Tsujimura
authors.forename: K
authors.initials: K
authors.lastname: Kuriyama
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D017931:DNA Primers; D026261:Diazepam Binding Inhibitor; D004305:Dose-Response Relationship, Drug; D005333:Fetus; D015870:Gene Expression / Q000187:drug effects*; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009538:Nicotine / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D026261:Diazepam Binding Inhibitor; D006584:Hexamethonium Compounds; D012333:RNA, Messenger; D018738:Hexamethonium; D009538:Nicotine