title: Differential effect of transforming growth factor-beta 1 on the activation of human naive and memory CD4+ T lymphocytes.
abstract: Transforming growth factor-beta 1 (TGF-beta 1) can have stimulatory or inhibitory effects on cell growth. For several cell types, the effect of TGF-beta 1 was found to correlate with the differentiation stage of the cells and the presence of other cytokines. We have studied here the influence of TGF-beta 1 on CD4+ T cell activation in relation to the differentiation stage of the cells by evaluating the effect of TGF-beta 1 on the proliferative responses of purified CD4+CD45RA+ (unprimed) and CD4+CD45RO+ (primed) lymphocytes. Under certain conditions, TGF-beta 1 exerted a co-stimulatory effect on peripheral blood CD4+CD45RA+ T cells whereas the outgrowth of CD4+CD45RO+ T cells was suppressed in any activation system tested. The enhancement of proliferative responses by TGF-beta 1 in TCR/CD3 or CD2 stimulated cultures of CD45RA+ cells involved up-regulation of CD25 expression and was dependent on the presence of exogenous IL-2 or CD28 mAbs; IL-7 driven proliferative responses were suppressed by TGF-beta 1. These observations were confirmed in experiments with purified cord blood (CB) CD4+ T cells inasmuch as addition of TGF-beta 1 caused a 2- to 7-fold increase in IL-2 driven proliferative responses of these cells. Finally we show that, in contrast to the effect of TGF-beta 1 during primary stimulation of CD CD4+ T cells, TGF-beta 1 suppressed T cell proliferation for approximately 40% in secondary cultures of these cells.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratoire d'Immunologie Cellulaire et Tissulaire, CNRS URA 625, CHU Pitié-Salpétrière, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: de Jong
authors.forename: R A
authors.initials: RA
authors.lastname: van Lier
authors.forename: F W
authors.initials: FW
authors.lastname: Ruscetti
authors.forename: C
authors.initials: C
authors.lastname: Schmitt
authors.forename: P
authors.initials: P
authors.lastname: Debré
authors.forename: M D
authors.initials: MD
authors.lastname: Mossalayi
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000097:blood*; D008213:Lymphocyte Activation / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000502:physiology*
chemical_list: D016212:Transforming Growth Factor beta; D017493:Leukocyte Common Antigens