Intrasplenic immunization with infected hepatocytes: a mouse model for studying protective immunity against malaria pre-erythrocytic stage.
Malaria liver forms are the target of antibody or T-cell-mediated immune mechanisms induced by previous or subsequent developmental stages of the parasite. The potential for vaccine development of antigens expressed exclusively in the liver stages has not been fully explored partly because of the lack of an experimental animal model. Here we show that protective immunity against sporozoite-induced infection with Plasmodium yoelii and P. berghei can be obtained by intrasplenic injection of a small number of liver stages of the parasites. The serum of the protected animals did not contain antibodies against sporozoites, liver or blood stage malaria parasites. Protective immunity was abolished by depletion of either CD4+ or CD8+ T cells from the vaccinated mice before challenge.
Rénia
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Michael Heidelberger Division of Immunology, Department of Pathology, New York University School of Medicine, New York.
Rodrigues
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MM
Nussenzweig
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V
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D007114:Immunization / Q000379:methods*; D008099:Liver / Q000469:parasitology*; D016031:Liver Transplantation / Q000276:immunology; D008288:Malaria / Q000517:prevention & control*; D017780:Malaria Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000469:parasitology; D010962:Plasmodium berghei / Q000276:immunology; D010967:Plasmodium yoelii / Q000254:growth & development / Q000276:immunology; D013154:Spleen / Q000469:parasitology
D000913:Antibodies, Protozoan; D016827:CD8 Antigens; D017780:Malaria Vaccines