Autoradiographic measurement of tyrosine hydroxylase immunoreactivity in rat brain using enhanced chemiluminescence: a general method for combined quantitative and cytochemical detection.
Autoradiographic measurements of peroxidase labelled tyrosine hydroxylase immunoreactivity in brain sections from rats with unilateral 6-hydroxydopamine lesions were obtained using enhanced chemiluminescence. The autoradiograms required only 5-60 s exposure and displayed a good signal-to-noise ratio. The autoradiograms were quantitated using densitometry and by comparison with a standard curve. Following acquisition of the autoradiograms the sections were stained with the chromogen diaminobenzidine (DAB). Distribution of the DAB closely followed the grain densities observed in the autoradiograms on both the lesioned and unlesioned side of the brain. This method represents a simple, rapid and inexpensive technique for performing both quantitative autoradiography and cytochemical studies in individual tissue sections of any peroxidase labelled immunoreactivity.
Norman
A B
AB
Department of Psychiatry, University of Cincinnati College of Medicine, OH 45267-0559.
Khosla
U M
UM
Klug
J M
JM
Thompson
H R
HR
D015100:3,3'-Diaminobenzidine; D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000166:cytology / Q000201:enzymology* / Q000473:pathology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods; D008163:Luminescent Measurements; D008297:Male; D016627:Oxidopamine / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D000911:Antibodies, Monoclonal; D015100:3,3'-Diaminobenzidine; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase