Differentiation effects of ciliary neurotrophic factor on human neuroblastoma cells.
In the human neuroblastoma cell line LA-N-2, recombinant rat ciliary neurotrophic factor (CNTF) induced neurite growth and cholinergic differentiation that were both half-maximally saturated at < 100 pM of the neurokine, but was not required for cell survival in serum-free conditions over a 13-day period. CNTF markedly stimulated choline acetyltransferase activity and acetylcholine synthesis, whereas high-affinity choline transport was only slightly enhanced and acetylcholinesterase activity was unchanged. Leukemia inhibitory factor had effects identical to CNTF on neurite growth and choline acetyltransferase activity, but interleukin 6 had no effect. Radioiodinated CNTF binding and affinity cross-linking studies were consistent with tripartite receptor activation as a mediator of the observed biological effects.
Lawrance
G
G
Department of Medicine, Queen's University, Kingston, Ontario, Canada.
Rylett
R J
RJ
Richardson
P M
PM
Dunn
R J
RJ
Dow
K E
KE
Riopelle
R J
RJ
D000110:Acetylcholinesterase / Q000378:metabolism; D001667:Binding, Competitive; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002794:Choline / Q000493:pharmacokinetics; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D020934:Ciliary Neurotrophic Factor; D003432:Cross-Linking Reagents; D006801:Humans; D009414:Nerve Growth Factors / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D020934:Ciliary Neurotrophic Factor; D003432:Cross-Linking Reagents; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; D002794:Choline