Evidence for the importance of iron in the alpha-oxidation of 3-methyl-substituted fatty acids in the intact cell.
Preincubation of isolated rat hepatocytes with desferrioxamine or o-phenanthroline, two iron-specific chelators, strongly suppressed the CO2-production from the alpha-oxidation of 3-methylmargaric acid, whereas the beta-oxidation of 2-methylpalmitic acid, palmitic acid, trihydroxycoprostanic acid and the conversion of formic acid to CO2 were not affected. When, after the initial preincubation with the chelators and prior to the addition of 3-methylmargaric acid, iron-saturated transferrin and Fe3+ were added, a partial restitution of the CO2-production rates was obtained. These facts provide further evidence for the importance of iron in the alpha-oxidation of 3-methyl-substituted fatty acids.
Croes
K
K
Katholieke Universiteit Leuven, Afdeling Farmacologie, Belgium.
Casteels
M
M
van Veldhoven
P P
PP
Mannaerts
G P
GP
D000818:Animals; D002478:Cells, Cultured; D002777:Cholestanols / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D002777:Cholestanols; D005227:Fatty Acids; D007502:Iron Chelating Agents; D010169:Palmitic Acids; C034839:2-methylhexadecanoic acid; D019308:Palmitic Acid; C033637:3-methylheptadecanoic acid; C022069:3,7,12-trihydroxycholestan-26-oic acid; D007501:Iron