Isolation and characterisation of five new anonymous X-specific probes. One of them detecting a TaqI RFLP.
The authors isolated five single-copy X-specific probes from an X-enriched library. These probes were regionally localized on the X chromosome by using somatic hybrid cell lines obtained from patients carrying different X-autosome translocations. Three clones were located between Xq23 and Xq26, the two others were mapped between Xp21 and Xp11.2. Analyses with different restriction enzymes indicated that one of them detects a TaqI polymorphism with a polymorphism information content (PIC) of 0.28.
Belhadj-Kacem
N
N
INSERM U12, Hôpital des Enfants-Malades, Paris, France.
Heuertz
S
S
Hors-Cayla
M C
MC
Saidi-Mehtar
N
N
Sefiani
A
A
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D015342:DNA Probes / Q000302:isolation & purification*; D005260:Female; D015723:Gene Library; D006801:Humans; D008297:Male; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D014178:Translocation, Genetic; D014960:X Chromosome / Q000737:chemistry*
D015342:DNA Probes