Interleukin-13 stimulates interleukin-6 production by human keratinocytes. Similarity with interleukin-4.
Interleukin-13 (IL-13) is a recently described human lymphokine which is produced by activated T-cells. Its effect on the production of IL-6 by normal keratinocytes and keratinocyte cell lines of human origin was studied and compared to that of IL-4. IL-13, similarly to IL-4, stimulated IL-6 expression by these cells in a dose- and time-dependent manner. Contamination with endotoxin was excluded by the use of polymyxin B and heat-inactivated cytokines. Further, we showed that IL-13, like IL-4, not only stimulated IL-6 production but also was able to induce overexpression of this cytokine in response to an inflammatory signal such as lipopolysaccharide (LPS). In a previous study, we demonstrated that IL-13, by inhibiting IL-6 and other cytokines produced by monocytes, exhibited an 'anti-inflammatory profile' comparable to that displayed by IL-4. In contrast, we show here that IL-13, by stimulating IL-6 production by keratinocytes, may favour the installation of an inflammatory process at a local level and, here again, it acted like IL-4. Therefore, according to the type of target cell these two 'TH2 type' cytokines induce similar opposing effects on IL-6 production and are likely to be important cytokines in the regulation of inflammation at both systemic and local levels.
Derocq
J M
JM
Sanofi Recherche, Immunology Department, Montpellier, France.
Segui
M
M
Poinot-Chazel
C
C
Minty
A
A
Caput
D
D
Ferrara
P
P
Casellas
P
P
D002460:Cell Line; D004731:Endotoxins / Q000494:pharmacology; D006801:Humans; D018793:Interleukin-13; D015847:Interleukin-4 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007378:Interleukins / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism
D004731:Endotoxins; D018793:Interleukin-13; D015850:Interleukin-6; D007378:Interleukins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D015847:Interleukin-4