title: Polycyclic aromatic hydrocarbon-DNA adducts in white blood cell DNA and 1-hydroxypyrene in the urine from aluminum workers: relation with job category and synergistic effect of smoking.
abstract: We examined a group of 105 workers from a primary aluminum plant for the presence of polycyclic aromatic hydrocarbon (PAH)-DNA adducts in their WBC and 1-hydroxypyrene in their urine. Workers were recruited from five job categories with different PAH exposure: the anode factory; the bake oven; and the electrolysis and the pot-relining departments. Unexposed workers from the foundry department served as the control group. The exposure to PAH was measured by personal monitoring, and the average PAH concentrations in the work atmosphere ranged from 0.4 micrograms/m3 in the foundry to 150 micrograms/m3 in the pot-relining department. The average exposure to benzo(a)pyrene was under the Swedish exposure limit of 5 micrograms/m3. The internal dose of pyrene was measured utilizing the 1-hydroxypyrene concentration in pre- and postshift urine samples. Higher exposure to PAH in the work atmosphere was associated with increased concentrations of 1-hydroxypyrene in the urine. The average increase in concentration of 1-hydroxypyrene ranged from 0.2 mumol/mol creatinine in the control group to 5.9 mumol/mol creatinine in the pot-relining department; an accumulation of 1-hydroxypyrene over a 5-day working period was observed. A good correlation was found between PAH exposure and the concentration of 1-hydroxypyrene in the urine on a group level (rs = 0.90; P = 0.02). PAH-DNA adducts were determined by 32P-postlabeling analysis (nuclease P1 enrichment procedure).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Health Risk Analysis and Toxicology, University of Limburg, Maastricht, Netherlands.
authors.forename: F J
authors.initials: FJ
authors.lastname: van Schooten
authors.forename: F J
authors.initials: FJ
authors.lastname: Jongeneelen
authors.forename: M J
authors.initials: MJ
authors.lastname: Hillebrand
authors.forename: F E
authors.initials: FE
authors.lastname: van Leeuwen
authors.forename: A J
authors.initials: AJ
authors.lastname: de Looff
authors.forename: A P
authors.initials: AP
authors.lastname: Dijkmans
authors.forename: J G
authors.initials: JG
authors.lastname: van Rooij
authors.forename: L
authors.initials: L
authors.lastname: den Engelse
authors.forename: E
authors.initials: E
authors.lastname: Kriek
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000032:analysis; D000535:Aluminum; D001564:Benzo(a)pyrene; D002617:Chemical Industry; D003404:Creatinine / Q000652:urine; D004247:DNA / Q000097:blood*; D018736:DNA Adducts / Q000097:blood*; D017575:Gloves, Protective; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008397:Masks; D008875:Middle Aged; D009153:Mutagens / Q000032:analysis*; D016273:Occupational Exposure; D010761:Phosphorus Radioisotopes; D011083:Polycyclic Compounds / Q000097:blood*; D011721:Pyrenes / Q000032:analysis*; D012044:Regression Analysis; D012907:Smoking / Q000097:blood* / Q000652:urine
chemical_list: D000395:Air Pollutants, Occupational; D018736:DNA Adducts; D009153:Mutagens; D010761:Phosphorus Radioisotopes; D011083:Polycyclic Compounds; D011721:Pyrenes; D001564:Benzo(a)pyrene; D004247:DNA; D003404:Creatinine; D000535:Aluminum; C033146:1-hydroxypyrene