Prenatal diagnosis of congenital human cytomegalovirus infection.
Fifteen fetuses at risk of congenital human cytomegalovirus (HCMV) infection underwent prenatal diagnosis at 16-30 weeks' gestation by a combination of amniocentesis and fetal blood sampling. HCMV was isolated from the amniotic fluid in six patients, but HCMV-specific IgM was detected in only three of them. Two of the nine neonates, who were delivered following a negative prenatal diagnosis, had congenital HCMV infection diagnosed by virus isolation in the urine. The interval from infection to prenatal testing was 3 and 4 weeks in the two false-negative cases and > or = 7 weeks in the true-positive cases. Although timely testing for HCMV infection allows the option of termination of pregnancy, it may be flawed by false-negative results.
Nicolini
U
U
First Department of Obstetrics and Gynaecology, University of Milano, Italy.
Kustermann
A
A
Tassis
B
B
Fogliani
R
R
Galimberti
A
A
Percivalle
E
E
Grazia Revello
M
M
Gerna
G
G
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000821:virology; D000914:Antibodies, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000097:blood / Q000151:congenital* / Q000175:diagnosis*; D005188:False Negative Reactions; D005260:Female; D005312:Fetal Blood / Q000201:enzymology / Q000276:immunology; D005315:Fetal Diseases / Q000097:blood / Q000175:diagnosis*; D005865:Gestational Age; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007231:Infant, Newborn; D011247:Pregnancy; D011296:Prenatal Diagnosis; D005723:gamma-Glutamyltransferase / Q000097:blood
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D005723:gamma-Glutamyltransferase