title: Conformational specificity of the chaperonin GroEL for the compact folding intermediates of alpha-lactalbumin.
abstract: The chaperonin GroEL binds unfolded polypeptides, preventing aggregation, and then mediates their folding in an ATP-dependent process. To understand the structural features in non-native polypeptides recognized by GroEL, we have used alpha-lactalbumin (alpha LA) as a model substrate. alpha LA (14.2 kDa) is stabilized by four disulfide bonds and a bound Ca2+ ion, offering the possibility of trapping partially folded disulfide intermediates between the native and the fully unfolded state. The conformers of alpha LA with high affinity for GroEL are compact, containing up to three disulfide bonds, and have significant secondary structure, but lack stable tertiary structure and expose hydrophobic surfaces. Complex formation requires almost the complete alpha LA sequence and is strongly dependent on salts that stabilize hydrophobic interactions. Unfolding of alpha LA to an extended state as well as the burial of hydrophobic surface upon formation of ordered tertiary structure prevent the binding to GroEL. Interestingly, GroEL interacts only with a specific subset of the many partially folded disulfide intermediates of alpha LA and thus may influence in vitro the kinetics of the folding pathways that lead to disulfide bonds with native combinations. We conclude that the chaperonin interacts with the hydrophobic surfaces exposed by proteins in a flexible compact intermediate or molten globule state.
authors.affiliation: Howard Hughes Medical Institute, Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
authors.forename: M K
authors.initials: MK
authors.lastname: Hayer-Hartl
authors.forename: J J
authors.initials: JJ
authors.lastname: Ewbank
authors.forename: T E
authors.initials: TE
authors.lastname: Creighton
authors.forename: F U
authors.initials: FU
authors.lastname: Hartl
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D018834:Chaperonin 60; D004220:Disulfides / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D007460:Iodoacetamide / Q000494:pharmacology; D007768:Lactalbumin / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation; D017510:Protein Folding; D012492:Salts / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004220:Disulfides; D006360:Heat-Shock Proteins; D010446:Peptide Fragments; D012492:Salts; D007768:Lactalbumin; D007460:Iodoacetamide