title: The NKB1 and HP-3E4 NK cells receptors are structurally distinct glycoproteins and independently recognize polymorphic HLA-B and HLA-C molecules.
abstract: NK cells lyse hematopoietic cells that lack expression of MHC class I molecules on the cell surface. Transfection of certain MHC class I negative cell lines with MHC class I genes renders these cells resistant to NK cell-mediated cytotoxicity. Recently, we described an NK cell receptor, NKB1, that inhibits NK cells from killing target cells expressing Bw4-reactive HLA-B molecules (-B*2705, -B*5101, -B*5801). In this study, we have demonstrated that another structurally distinct NK cell membrane glycoprotein, HP-3E4, is involved in the recognition of certain polymorphic HLA-C molecules (-Cw*0401 and -Cw*1503). NK cell clones co-expressing both the NKB1 and HP-3E4 receptors fail to lyse targets expressing HLA-Cw*0401 and -B*5801, but are able to kill the transfectants in the presence of mAbs against both receptors. These studies demonstrate that a single NK cell clone may possess multiple structurally distinct receptors for different polymorphic HLA class I molecules that function independently.
authors.affiliation: Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304.
authors.forename: L L
authors.initials: LL
authors.lastname: Lanier
authors.forename: J E
authors.initials: JE
authors.lastname: Gumperz
authors.forename: P
authors.initials: P
authors.lastname: Parham
authors.forename: I
authors.initials: I
authors.lastname: Melero
authors.forename: M
authors.initials: M
authors.lastname: López-Botet
authors.forename: J H
authors.initials: JH
authors.lastname: Phillips
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D006023:Glycoproteins / Q000276:immunology; D015235:HLA-B Antigens / Q000276:immunology*; D015236:HLA-C Antigens / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D011971:Receptors, Immunologic / Q000737:chemistry / Q000276:immunology*; D054340:Receptors, KIR; D054341:Receptors, KIR3DL1; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054341:Receptors, KIR3DL1