DNA typing of HLA-B gene in Takayasu's arteritis.
Sixty-four patients with Takayasu's arteritis and 156 healthy individuals in the Japanese population were examined for HLA-B specificity at the DNA level by DNA typing using polymerase chain reaction (PCR)/sequence-specific oligonucleotide probe (SSOP) analysis and by subsequent sequencing analysis. The frequency of epitope combination group-B52 (EC-B52) corresponding precisely to HLA-B52 specificity and that of EC-B39.2 which is a newly-identified subtype of HLA-B39 specificity were increased in the patient group. These two disease-associated HLA-B alleles share an epitope composed of 63Glu and 67Ser. Because two HLA-B alleles, HLA-B51 and B39.1, which are similar but different at the epitope from HLA-B52 and B39.2, respectively, are not associated with Takayasu arteritis, 63Glu and 67Ser are supposed to be involved in the pathogenesis.
Yoshida
M
M
Department of Genetics, Kyushu University, Fukuoka, Japan.
Kimura
A
A
Katsuragi
K
K
Numano
F
F
Sasazuki
T
T
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D005260:Female; D005787:Gene Frequency; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D015235:HLA-B Antigens / Q000235:genetics*; D059946:HLA-B39 Antigen; D059950:HLA-B52 Antigen; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013625:Takayasu Arteritis / Q000235:genetics*
D015235:HLA-B Antigens; D059946:HLA-B39 Antigen; D059950:HLA-B52 Antigen