title: Effect of vitamin D deficiency and 1,25-dihydroxyvitamin D3 on rat heart metabolism.
abstract: The purpose of this study was to investigate whether vitamin D3 deficiency and 1,25-dihydroxyvitamin D3 treatment affect some aspects of heart metabolism in the rat. To this end, five experimental groups were studied: (1) the control group of the vitamin D3 supplemented rats (Group A); (2) rachitic rats (Group B); (3) rachitic rats treated with 1,25-dihydroxyvitamin D3 (Group C); (4) rats fed a vitamin D-deficient diet (Group D); (5) rats fed a vitamin D-deficient diet and treated with 1,25-dihydroxyvitamin D3 (Group E). The five groups were compared by checking in the heart some metabolic parameters, i.e. citrate content, and enzyme activities in cytosol and mitochondria. Citrate content was higher in the heart of treated animals when compared with the control. As regards the enzymatic activities in heart mitochondria, NAD(+)-dependent isocitrate dehydrogenase remarkably decreased in Group B rats and 1,25-dihydroxyvitamin D3 restored quite normal values. NADP(+)-dependent isocitrate dehydrogenase decreased in Group B and Group D animals, and 1,25-dihydroxyvitamin D3 treatment was effective in restoring control values. Cytochrome c oxidase activity did not change, while citrate synthase showed an increase in all the treated rats. As regards the cytosolic enzymes, fructose-6-phosphate kinase increased in the two groups of vitamin D-deplete rats in comparison with the control. Glyceraldehyde-3-phosphate dehydrogenase and 3-phosphoglycerate kinase showed a similar trend: an increase in all the treated animals. In heart homogenate, acylphosphatase and acid phosphatase activities were also determined. Acylphosphatase increased in the treated rats, while acid phosphatase decreased in the rats injected with 1,25-dihydroxyvitamin D3. These results support the hypothesis of a participation of 1,25-dihydroxyvitamin D3 in some aspects of heart metabolism.
authors.affiliation: Department of Biochemical Sciences, University of Florence, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Stio
authors.forename: B
authors.initials: B
authors.lastname: Lunghi
authors.forename: T
authors.initials: T
authors.lastname: Iantomasi
authors.forename: M T
authors.initials: MT
authors.lastname: Vincenzini
authors.forename: C
authors.initials: C
authors.lastname: Treves
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000494:pharmacology* / Q000627:therapeutic use; D002118:Calcium / Q000378:metabolism; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D006321:Heart / Q000187:drug effects*; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology; D009124:Muscle Proteins / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014808:Vitamin D Deficiency / Q000188:drug therapy / Q000378:metabolism*
chemical_list: D002951:Citrates; D009124:Muscle Proteins; D010758:Phosphorus; D019343:Citric Acid; D002117:Calcitriol; D002118:Calcium