[The degree of collagen crosslinks in medical collagen membranes determined by measuring their water absorption].
Collagen membranes were crosslinked by using three agents: glutaraldehyde, hexamethylene-diisocyanate, and UV irradiation. The increasing concentrations of above chemical agents or longer time of UV exposition resulted in the higher cross-links degree and in the decrease of collagen membranes swelling (measured as water absorption), their elasticity and mechanical resistance. According to American standards, the degree of collagen biomaterial cross-links is determined by measuring of the digestion time by pepsin. However, that method is very time-consuming. In our study, we have found that a simple, linear regression between logarithm of digestion time by pepsin exists and it was identical for all three cross-linking agents used. We have concluded that determination of water absorption can be an alternative, simple and fast method for examination of collagen membrane cross-links degree.
Braczko
M
M
Instytut Przemysłu Miesnego i Tłuszczowego w Warszawie.
Tederko
A
A
Grzybowski
J
J
D000042:Absorption; D000818:Animals; D001696:Biomechanical Phenomena; D002417:Cattle; D003094:Collagen / Q000737:chemistry* / Q000528:radiation effects; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004548:Elasticity; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D016014:Linear Models; D010434:Pepsin A / Q000737:chemistry; D012044:Regression Analysis; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry*
D003432:Cross-Linking Reagents; D014867:Water; D003094:Collagen; D010434:Pepsin A
Określanie stopnia usieciowania błon kolagenowych przeznaczonych do celów medycznych metoda badania ich wodochłonności.