Nicotine increases diazepam binding inhibitor (DBI) mRNA in primary cultured neurons.
The effect of nicotine on the expression of diazepam binding inhibitor (DBI) mRNA in primary cultured cerebral cortical neurons was examined using Northern blot analysis. Nicotine exposure (0.001-10 microM) for 24 h increased the DBI mRNA level in a dose-dependent manner, whereas the beta-actin mRNA level showed no change. This effect of nicotine was faded out over 48 h of its exposure. Hexamethonium (100 microM) completely abolished the nicotine-induced increase in DBI mRNA expression. These results indicate that nicotine increases the expression of DBI mRNA in cerebral cortical neurons via the activation of nicotinic acetylcholine receptor.
Katsura
M
M
Department of Pharmacology, Kyoto Prefectural University of Medicine, Japan.
Ohkuma
S
S
Chen
D T
DT
Tsujimura
A
A
Kuriyama
K
K
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D017931:DNA Primers; D026261:Diazepam Binding Inhibitor; D004305:Dose-Response Relationship, Drug; D005333:Fetus; D015870:Gene Expression / Q000187:drug effects*; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009538:Nicotine / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*
D002352:Carrier Proteins; D017931:DNA Primers; D026261:Diazepam Binding Inhibitor; D006584:Hexamethonium Compounds; D012333:RNA, Messenger; D018738:Hexamethonium; D009538:Nicotine