A new method for direct analysis of polymerase chain reaction-amplified human papillomavirus using DNA enzyme immunoassay.
We describe use of a polymerase chain reaction in conjunction with a DNA enzyme immunoassay for the simultaneous detection of multiple types of human papillomavirus in a single microtiter plate. The method is specific, sensitive, rapid, and capable of detecting human papillomavirus-6, -11, -16, -18, and -33 in cervical biopsy specimens.
Sangiuolo
F
F
Department of Human Genetics, Tor Vergata University of Rome, Italy.
De Santis
L
L
Cavicchini
A
A
Angeloni
U
U
Romanini
C
C
Novelli
G
G
Dallapiccola
B
B
D001483:Base Sequence; D015344:DNA Probes, HPV; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
D015344:DNA Probes, HPV