title: Anaphylactic colonic hypersecretion in cow's milk sensitized guinea-pigs depends upon release of interleukin-1, prostaglandins and mast cell degranulation.
abstract: METHODS
  The effect of beta-lactoglobulin (beta-LGI) challenge on net water movements into the proximal colon and the role of Interleukin-1 (IL-1), prostaglandins and mast cell degranulation on the challenge-induced net water changes were assessed in vivo using isolated colonic loops in anaesthetized guinea-pigs immunized to bovine milk.
  
  
  RESULTS
  beta-lactoglobulin challenge infused into the colonic loop during 30 min reversed the net water flux into a net secretion during the period of antigen infusion. Doxantrazole, a mast cell stabilizing agent, administered 120 min before challenge infusion, suppressed challenge-induced hypersecretion. Similarly recombinant IL-1 receptor antagonist protein abolished the antigen-induced colonic secretory effect. Indomethacin, a prostaglandin synthesis inhibitor, administered 20 min prior to antigen infusion, significantly (P < 0.05) reduced, but did not abolish, the challenge-induced colonic secretory effect.
  
  
  CONCLUSIONS
  These results suggest that IL-1 plays an important role in antigen challenge-induced colonic hypersecretion which involves mast cell degranulation and prostaglandin release.
authors.affiliation: Department of Pharmacology, Institut National de la Recherche Agronomique, Toulouse, France.
authors.forename: V
authors.initials: V
authors.lastname: Theodorou
authors.forename: J
authors.initials: J
authors.lastname: Fioramonti
authors.forename: J L
authors.initials: JL
authors.lastname: Junien
authors.forename: L
authors.initials: L
authors.lastname: Bueno
mesh_terms: D000707:Anaphylaxis / Q000378:metabolism; D000818:Animals; D015550:Cell Degranulation / Q000502:physiology*; D003106:Colon / Q000187:drug effects / Q000378:metabolism*; D006168:Guinea Pigs; D007213:Indomethacin / Q000494:pharmacology*; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000502:physiology*; D007408:Intestinal Absorption / Q000187:drug effects*; D007782:Lactoglobulins / Q000009:adverse effects*; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D016269:Milk Hypersensitivity / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000494:pharmacology; D013892:Thioxanthenes / Q000494:pharmacology*; D013997:Time Factors; D014867:Water / Q000378:metabolism; D044004:Xanthones
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D007782:Lactoglobulins; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D013892:Thioxanthenes; D044004:Xanthones; D014867:Water; C010134:doxantrazole; D007213:Indomethacin