In situ detection of PCR-amplified HIV-1 nucleic acids in lymph nodes and peripheral blood in patients with asymptomatic HIV-1 infection and advanced-stage AIDS.
This study determined the in situ detection rate of polymerase chain reaction (PCR)-amplified human immunodeficiency virus type 1 (HIV-1) DNA and RNA in lymph nodes and peripheral blood CD4+ cells in six patients with asymptomatic HIV-1 infection and from six people who died of advanced AIDS. The lymph nodes of patients with asymptomatic infection showed expanded germinal centers where, on average, 20% of the CD21+ dendritic cells contained HIV-1 DNA. From 5 to 80% of the CD4+ cells in these lymph nodes contained HIV-1 DNA, as compared with 1-11% of the CD4+ peripheral blood mononuclear cells. The infection in most cells was latent in the asymptomatic group. In contrast, the lymph nodes of patients with advanced AIDS showed marked depletion of both dendritic and CD4+ cells. The majority of the remaining CD4+ cells in the lymph nodes and blood showed PCR-amplified viral DNA and cDNA sequences suggesting the presence of genomic and multiple spliced transcripts. It is concluded that asymptomatic HIV-1 infection is associated with a wide range of latent to active viral-positive CD4+ lymphocytes and dendritic cells in the lymph nodes. Progression to AIDS is characterized by active viral replication in many of the remaining CD4+ cells in the lymph nodes and blood.
Nuovo
G J
GJ
Department of Pathology, SUNY at Stony Brook 11794-8691.
Becker
J
J
Burk
M W
MW
Margiotta
M
M
Fuhrer
J
J
Steigbigel
R T
RT
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood; D015658:HIV Infections / Q000382:microbiology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008198:Lymph Nodes / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis* / Q000097:blood
D004279:DNA, Viral; D012367:RNA, Viral