Biotinylated platelets: a new approach to the measurement of platelet life span.
The in vivo life span of dog platelets was determined by derivatizing whole blood with N-hydroxysuccinimido biotin, reinfusing the biotinylated blood and subsequently monitoring the survival of the biotinylated platelets with flow cytometry. We found that the biotinylated platelets had a mean life span of 6.0 +/- 1.1 d as determined by curve-fitting the platelet disappearance data to gamma functions. These data are in good agreement with literature values of platelet life span for canine platelets labelled with either 111Indium-oxine or 51Chromium. Biotinylated platelets were analysed after reinfusion and found to aggregate normally in response to the agonists adenosine diphosphate and phorbol myristate acetate. These experiments demonstrate that biotinylated platelets survive normally in vivo and that this labelling method can be used for determining platelet life spans.
Heilmann
E
E
Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Friese
P
P
Anderson
S
S
George
J N
JN
Hanson
S R
SR
Burstein
S A
SA
Dale
G L
GL
D000818:Animals; D001710:Biotin / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology*; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology; D004285:Dogs; D005260:Female; D005434:Flow Cytometry; D008297:Male; D015539:Platelet Activation; D010974:Platelet Aggregation
D001710:Biotin