title: Soluble intracellular adhesion molecule 1 in bronchoalveolar lavage fluid of allergic subjects following segmental antigen challenge.
abstract: This study was undertaken to determine the relationship of soluble intercellular adhesion molecule 1 (sICAM-1) levels in bronchoalveolar lavage (BAL) fluid during allergic airway inflammation to those in the vascular compartment and to cellular components in the BAL fluids. A group of 11 allergic subjects underwent initial bronchoscopy during which a control BAL was performed and normal saline (NS) and specific antigen (Ag) were administered to two sublobar segments. A second bronchoscopy was performed 17 to 21 h later, and the NS and Ag segments were lavaged. Blood was drawn before each bronchoscopic procedure. The mean concentration of sICAM-1 in BAL fluid from NS-challenged segments was 59.2 +/- 7.6 ng/ml and was not different from that in unchallenged segments (51.5 +/- 5.6 ng/ml). In BAL fluid from Ag-challenged segments, mean concentrations of sICAM-1 increased significantly to 97.5 +/- 12.5 ng/ml. Segmental antigen challenge was associated with a small but statistically significant increase in sICAM-1 concentrations in serum. The concentrations of sICAM-1 in BAL fluid after antigen challenge exceeded levels that could be accounted for by passive transudation from the circulation, based upon the magnitude of increases in BAL albumin concentrations. The levels of sICAM-1 in BAL from Ag-challenged segments were correlated significantly with the total white cell, lymphocyte, neutrophil, and eosinophil counts in BAL fluids. These results are supportive of the notion that the local release of sICAM-1 may play a role in allergen-induced inflammatory processes in the airways.
authors.affiliation: Department of Environmental Health Sciences, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 21205.
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: M C
authors.initials: MC
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Proud
authors.forename: X Y
authors.initials: XY
authors.lastname: Yu
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
authors.forename: E W
authors.initials: EW
authors.lastname: Spannhake
mesh_terms: D000328:Adult; D000485:Allergens; D000704:Analysis of Variance; D015703:Antigens, CD / Q000032:analysis*; D001249:Asthma / Q000453:epidemiology / Q000276:immunology*; D001985:Bronchial Provocation Tests / Q000379:methods / Q000706:statistics & numerical data; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D001999:Bronchoscopy; D015815:Cell Adhesion Molecules / Q000032:analysis*; D005260:Female; D005336:Fiber Optic Technology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D008297:Male; D006255:Rhinitis, Allergic, Seasonal / Q000453:epidemiology / Q000276:immunology*; D012882:Skin Tests; D012995:Solubility
chemical_list: D000485:Allergens; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1