Application of a recombinant baculovirus expressing hantavirus nucleocapsid protein as a diagnostic antigen in IFA test: cross reactivities among 3 serotypes of hantavirus which causes hemorrhagic fever with renal syndrome (HFRS).
Recombinant baculovirus-infected insect cells which expressed recombinant protein, analogous to the nucleocapsid protein (NP) of Seoul type hantavirus, strain SR-11 (rNP-SR-Sf9) were applied to the indirect immunofluorescent antibody (IFA) test. The rNP-SR-Sf9 reacted with anti NP MAb clones which recognized strain specific or hantavirus common epitopes in the IFA test. The recombinant antigen was detected by antibodies to 3 serotypes of hantaviruses (Hantaan 76-118, SR-11, Puumala). Antibody titers of a group of experimentally infected mouse and urban rat sera using rNP-SR-Sf9 and strain SR-11-infected Vero cell antigens were mutual correlative. These results indicated that the rNP-SR-Sf9 were an effective and safety substitute for Vero E6 cells in the IFA test for serosurveys of hantavirus infection.
Yoshimatsu
K
K
Institute of Immunological Science, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Arikawa
J
J
Kariwa
H
H
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D016367:Baculoviridae / Q000235:genetics*; D002213:Capsid / Q000032:analysis* / Q000096:biosynthesis; D002460:Cell Line; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006476:Orthohantavirus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006480:Hemorrhagic Fever with Renal Syndrome / Q000175:diagnosis*; D007674:Kidney Diseases / Q000382:microbiology*; D051379:Mice; D009036:Moths; D051381:Rats; D013577:Syndrome; D014709:Vero Cells
D000956:Antigens, Viral