A simple method for genetic differentiation of the AIK-C vaccine strain from wild strains of measles virus.
Restriction fragment length polymorphism (RFLP) analysis was used to differentiate the AIK-C vaccine strain from the wild circulating measles viruses. Virus genomes were amplified by using reverse transcriptase-polymerase chain reaction (RT-PCR) in the part of the H-gene. The PCR product of the AIK-C strain was cut into 2 fragments after digestions with Hpall, BstPl and Avall, respectively. Otherwise, those of 17 wild-type strains isolated during 1950s-1991 were cleaved into 3 fragments only when digested with Avall. Comparison with wild strain sequences suggest that the above Hpall site and BstPl site are characteristic of the AIK-C strain. This molecular approach is also applied for the discrimination whether some symptoms after vaccination were related to the vaccine strain or not.
Mori
T
T
Department of Virology, Kitasato Institute, Tokyo, Japan.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D007223:Infant; D008458:Measles Vaccine / Q000302:isolation & purification*; D008459:Measles virus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014709:Vero Cells
D004279:DNA, Viral; D008458:Measles Vaccine