title: Separation of lipophilic compounds by micellar electrokinetic chromatography with organic modifiers.
abstract: Separation of lipophilic compounds such as polyaromatic hydrocarbons (PAH) by micellar electrokinetic chromatography (MEKC) with organic modifiers was investigated. Dimethyl sulfoxide (DMSO) and acetone were used as organic modifiers, and sodium dodecyl sulfate (SDS) as a surfactant or a micelle forming reagent. By using 25 mM SDS (pH 7.0), containing 50% v/v DMSO, 8 PAHs were separated. Similarly, with 25 mM SDS, containing 30% acetone, 13 PAHs were successfully separated. For the calculation of thermodynamic quantities, critical micelle concentrations of SDS in buffers containing DMSO or acetone were measured.
authors.affiliation: Department of Industrial Chemistry, Osaka Prefectural College of Technology, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Otsuka
authors.forename: M
authors.initials: M
authors.lastname: Higashimori
authors.forename: R
authors.initials: R
authors.lastname: Koike
authors.forename: K
authors.initials: K
authors.lastname: Karuhaka
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: S
authors.initials: S
authors.lastname: Terabe
mesh_terms: D000096:Acetone; D002845:Chromatography / Q000379:methods*; D004121:Dimethyl Sulfoxide; D004563:Electrochemistry; D004586:Electrophoresis / Q000379:methods*; D008823:Micelles; D011083:Polycyclic Compounds / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D008823:Micelles; D011083:Polycyclic Compounds; D000096:Acetone; D012967:Sodium Dodecyl Sulfate; D004121:Dimethyl Sulfoxide