Expression and distribution of transforming growth factor-beta and decorin during fracture healing.
To study interactions between TGF-beta and decorin, we experimentally induced femoral fracture in rats and immunohistologically examined changes in the distribution of TGF-beta and decorin in the femur 3, 7 and 14 days after fracture. In the areas where endochondral ossification occurred, decorin was detected during differentiation of mesenchymal cells into proliferating chondrocytes, while no TGB-beta was observed during same period. Once chondrocytes had hypertrophied, decorin was no longer observed, while the matrix around the hypertrophic chondrocytes was positively stained for TGB-beta. The disappearance of decorin, almost simultaneously accompanied by the appearance of the active type TGB-beta, suggests that decorin regulates the actions of TGB-beta.
Matsumoto
K
K
Department of Orthopaedics, Faculty of Medicine, Kagoshima University, Japan.
Matsunaga
S
S
Imamura
T
T
Ishidou
Y
Y
Yoshida
H
H
Sakou
T
T
D000818:Animals; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005264:Femoral Fractures / Q000378:metabolism* / Q000503:physiopathology; D017102:Fracture Healing / Q000502:physiology; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D016212:Transforming Growth Factor beta