Enhanced staining of bacterial flagella using aged mordant in the silver stain.
Intensity of bacterial flagella staining using a modified silver stain was increased by aging the mordant for one week at room temperature. The use of aged mordant increased the apparent diameters of stained flagella and resulted in a darker stain. The mordant remained stable for at least four months at room temperature. The staining protocol presented allows application to liquid or solid cultures.
Finegan
S M
SM
Department of Biological Sciences, Philadelphia College of Pharmacy and Science, Pennsylvania 19104-4495.
Smith
R A
RA
D001412:Bacillus subtilis / Q000648:ultrastructure; D005407:Flagella / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000648:ultrastructure*; D006094:Gram-Positive Bacteria / Q000648:ultrastructure*; D008358:Mannose / Q000494:pharmacology; D011513:Proteus mirabilis / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000648:ultrastructure; D012706:Serratia marcescens / Q000648:ultrastructure; D016622:Silver Staining / Q000379:methods*
D005947:Glucose; D008358:Mannose