Substance P receptor-immunoreactive neurons in the rat neostriatum are segregated into somatostatinergic and cholinergic aspiny neurons.
Immunochemical characteristics of neostriatal neurons producing substance P receptor (SPR) were examined in adult rats by double- and triple-immunofluorescence methods. In the neostriatum, SPR immunoreactivity was detected in large and medium-sized aspiny neurons. Virtually all SPR-immunoreactive neurons in the neostriatum contained somatostatin (SS) or choline acetyltransferase (ChAT), but not parvalbumin. All SS- and ChAT-immunoreactive neurons in the neostriatum showed SPR immunoreactivity. The co-existence of SS and ChAT was, however, not found in single neurons expressing SPR immunoreactivity. The present results indicate that neostriatal neurons immunoreactive for SPR are segregated into 2 groups: (1) medium-sized, spiny somatostatinergic, and (2) large, aspiny cholinergic neurons.
Kaneko
T
T
Department of Morphological Brain Science, Faculty of Medicine, Kyoto University, Japan.
Shigemoto
R
R
Nakanishi
S
S
Mizuno
N
N
D000818:Animals; D015415:Biomarkers; D002795:Choline O-Acetyltransferase / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D017072:Neostriatum / Q000166:cytology* / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D010275:Parasympathetic Nervous System / Q000166:cytology* / Q000201:enzymology / Q000502:physiology; D010320:Parvalbumins / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000276:immunology / Q000378:metabolism*; D013004:Somatostatin / Q000276:immunology / Q000502:physiology*
D015415:Biomarkers; D010320:Parvalbumins; D018040:Receptors, Neurokinin-1; D013004:Somatostatin; D002795:Choline O-Acetyltransferase