title: The accessory colonization factor and toxin-coregulated pilus gene clusters are physically linked on the Vibrio cholerae 0395 chromosome.
abstract: A partial nucleotide sequence of the first gene in the Vibrio cholerae accessory colonization factor gene cluster was obtained by sequencing a cloned acfB::TnphoA fusion junction. Analysis of the sequence data revealed that the ATG start codon of the acfB structural gene is located 6 bp downstream of the tcpJ TGA stop codon. This establishes a large region in excess of 20 kbp of the Vibrio cholerae chromosome as a cluster of coordinately regulated virulence genes involved in intestinal colonization. The absence of a characteristic transcription termination site in the tcpJ-acfB intergenic region indicates that acfB may be encoded on the same messenger RNA as tcpJ. The deduced amino acid sequence for the N-terminal 143 amino acids of AcfB reveals a potential leader peptide, that lacks a consensus signal peptidase I recognition sequence. Computer algorithms fail to detect any significant similarities between the partial AcfB amino acid sequence and any entries in the SWISS-PROT database.
authors.affiliation: Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130-3932.
authors.forename: K D
authors.initials: KD
authors.lastname: Everiss
authors.forename: K J
authors.initials: KJ
authors.lastname: Hughes
authors.forename: K M
authors.initials: KM
authors.lastname: Peterson
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002772:Cholera Toxin / Q000378:metabolism; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial; D010861:Fimbriae, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008040:Genetic Linkage; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D005810:Multigene Family; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: C088551:AcfB protein, Vibrio cholerae; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D002772:Cholera Toxin; C092637:Tcpj protein, Vibrio cholerae