Reversed-phase high-performance liquid chromatographic determination of taxol in mouse plasma.
A simple and selective high-performance liquid chromatographic technique was developed for the determination of taxol in mouse plasma. This technique employs a single extraction step followed by isocratic chromatography on a C18 analytical column and ultraviolet detection at 227 nm, and is suitable for the analysis of microsamples (50 microliters plasma). A series of aromatic and aliphatic benzamides were synthesized as internal standards; N-octylbenzamide was evaluated most extensively. The calibration curve is linear within the range 0.15-10 nmol/ml. The intra-day and inter-day coefficients of variation were below 7%. The specificity of the assay was confirmed by comparing the chromatographic properties of samples with authentic taxol, using a diode-array detector. The assay was used to conduct pharmacokinetic studies of taxol in mice after administration in different formulations.
Sharma
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Department of Pharmaceutics, University at Buffalo, State University of New York, Amherst 14260-1200.
Conway
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WD
Straubinger
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RM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D008081:Liposomes / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D017239:Paclitaxel / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D014677:Pharmaceutical Vehicles; D011092:Polyethylene Glycols
D008081:Liposomes; D014677:Pharmaceutical Vehicles; C022131:cremophor; D011092:Polyethylene Glycols; D017239:Paclitaxel