Cellular subsets and epithelial ICAM-1 and HLA-DR expression in human papillomavirus infection of the vulva.
Cryostat sections of 20 clinical condylomata of the vulva induced by human papillomavirus and 5 normal control biopsies were examined using immunohistochemistry. The results indicated that in vulvar papillomavirus infection the intraepithelial Langerhans' cells showed abnormal morphology and a significantly lower density than controls. CD1a positive Langerhans' cells were also observed in dermis of condylomata, suggesting an abnormal epithelial traffic of dendritic cells. T lymphocytes with a mean CD4/CD8 ratio of 0.25 and a mean density of 267 +/- 59 cells/mm2 of epithelial section were the main cellular infiltrate in vulvar papillomavirus infection. Most of the T cells were HLA-DR negative. Those condylomata with moderate to severe mononuclear infiltrate showed leucocyte function antigen 1 positive T cells forming small clusters in the lower epithelial half around the ICAM-1 positive keratinocytes. Vulvar warts also showed epithelial areas with overlapped ICAM-1 and HLA-DR expression. Scattered T gamma-delta and B lymphocytes, macrophages and NK cells were observed among the cells of the dermal infiltrate of vulvar condylomata.
Morelli
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AE
Department of Immunogenetics, Hospital de Clínicas, José de San Martín, School of Medicine, University of Buenos Aires, Argentina.
Belardi
G
G
DiPaola
G
G
Paredes
A
A
Fainboim
L
L
D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis; D016516:CD4-CD8 Ratio; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D003218:Condylomata Acuminata / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D004848:Epithelium / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006684:HLA-DR Antigens / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1; D007694:Killer Cells, Natural / Q000378:metabolism / Q000473:pathology; D007801:Langerhans Cells / Q000378:metabolism / Q000473:pathology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D016176:T-Lymphocyte Subsets / Q000378:metabolism / Q000473:pathology; D014845:Vulvar Diseases / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000821:virology
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D006684:HLA-DR Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1