title: Detection of proliferating cell nuclear antigen in gliomas and adjacent resection margins.
abstract: We describe a technique for estimating the number of proliferating cells in gliomas and adjacent resection cavities after tumor removal. Proliferating cell nuclear antigen (PCNA) is a nuclear protein associated with the cell cycle. Anti-PCNA antibody staining provides a semiquantitative estimate of the number of proliferating cells found in fixed tissue embedded in paraffin. The extent of the staining of tumor cells of glioblastoma multiforme, anaplastic astrocytomas, low-grade gliomas, and other lesions of the brain with anti-PCNA antibody is correlated with the histological diagnosis. In addition, the labeling of the margins after resection of gliomas and other lesions with anti-PCNA antibody is also associated with the histological diagnosis of the lesion. This technique may be useful in estimating the "biological" extent of resection and in predicting the recurrence patterns of gliomas.
authors.affiliation: Department of Neurological Surgery, University of Washington School of Medicine, Seattle.
authors.forename: D K
authors.initials: DK
authors.lastname: Kim
authors.forename: J
authors.initials: J
authors.lastname: Hoyt
authors.forename: C
authors.initials: C
authors.lastname: Bacchi
authors.forename: G E
authors.initials: GE
authors.lastname: Keles
authors.forename: M
authors.initials: M
authors.lastname: Mass
authors.forename: M R
authors.initials: MR
authors.lastname: Mayberg
authors.forename: M S
authors.initials: MS
authors.lastname: Berger
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000473:pathology* / Q000601:surgery; D002455:Cell Division / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005910:Glioma / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000473:pathology / Q000601:surgery; D009687:Nuclear Proteins / Q000032:analysis*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen
chemical_list: D000951:Antigens, Neoplasm; D005904:Glial Fibrillary Acidic Protein; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen