Stability of a low molecular mass cytotoxin from the sea anemone Radianthus macrodactylus and membrane-toxin interactions.
Differential scanning calorimetry, intrinsic fluorescence and SDS-polyacrylamide-gel-electrophoresis have been applied for the study of the low molecular mass cytotoxin from the sea anemone, Radianthus macrodactylus when in solution and in complexes with phospholipids and erythrocyte membranes. The thermodynamic parameters of toxin denaturation in solution have been obtained. It is shown that one molecule of this toxin withdraws 10 +/- 2 dipalmitoylphosphatidylcholine molecules through cooperative transition. The effect of toxin on dog erythrocytes causes a change in the polypeptide contents of erythrocyte ghosts.
Zhadan
G
G
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino Moscow Region.
Kuznetsova
S
S
Opalikova
O
O
Monastyrnaya
M
M
Zykova
T
T
Emelyanenko
V
V
Villar
E
E
Shnyrov
V
V
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D003064:Cnidarian Venoms / Q000737:chemistry*; D003603:Cytotoxins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D004285:Dogs; D004355:Drug Stability; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000378:metabolism*; D008970:Molecular Weight; D011489:Protein Denaturation; D012615:Sea Anemones / Q000737:chemistry*; D013816:Thermodynamics
D000596:Amino Acids; D003064:Cnidarian Venoms; D003603:Cytotoxins; D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine