title: Distribution of histamine H3 binding in forebrain of mouse and guinea pig.
abstract: To map the unknown distribution of histamine H3 binding sites, we compared autoradiographs of the binding of the H3 agonist N alpha-[3H]methylhistamine ([3H]NAMH) in the forebrain of mouse and guinea pig. Saturation binding revealed the presence of a single site with apparent affinity of 0.75 nM in the mouse and 1.2 nM in the guinea pig. Binding constants were nearly identical in male and female mice. The rank order of [3H]NAMH binding in mouse brain was striatum > insular cortex > neocortex, as previously observed in the rat. In the guinea pig, the rank order was insular cortex > striatum approximately neocortex. The Bmax of [3H]NAMH in insular cortex of both species was close to 60 fmol/mg. Thus, the guinea pig differs from the rodent in that the highest density of H3 binding was present in cortical rather than subcortical structures.
authors.affiliation: Montreal Neurological Institute, Que., Canada.
authors.forename: P
authors.initials: P
authors.lastname: Cumming
authors.forename: C
authors.initials: C
authors.lastname: Laliberté
authors.forename: A
authors.initials: A
authors.lastname: Gjedde
mesh_terms: D000818:Animals; D001345:Autoradiography; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D016548:Prosencephalon / Q000737:chemistry*; D011869:Radioligand Assay; D018100:Receptors, Histamine H3 / Q000032:analysis*; D012727:Sex Characteristics; D013045:Species Specificity
chemical_list: D018100:Receptors, Histamine H3