Functional analysis of human bronchial mucosal T cells extracted with interleukin-2.
Functional analysis of T cells from the bronchial mucosa has been limited by difficulties in extracting T cells from this tissue. Because interleukin-2 (IL-2) is chemotactic for T cells, we determined whether this cytokine could be used to extract T cells from human bronchial wall (BW). Fresh tissue was obtained from 21 patients undergoing surgery for malignancy. Within the BW, 95% of T cells stained for the memory/activation marker CD45RO. When BW sections were incubated with IL-2 for 24 h, 88 to 91% of T cells emigrated into the culture medium. Compared with autologous blood T cells (also exposed to IL-2), these BW T cells expressed CD2 at a greater intensity and showed a fourfold reduction in cloning efficiency in response to phytohemagglutinin, and T-cell clones derived from the BW population displayed a tendency for higher interferon-gamma production. Furthermore, we were also able to extract and clone T cells from bronchoscopic biopsies in four subjects, suggesting that this method will provide a new avenue for examining T-cell function in airway inflammatory diseases.
Upham
J W
JW
Western Australian Research Institute for Child Health, Subiaco.
McMenamin
C
C
Schon-Hegrad
M A
MA
Robinson
B W
BW
Holt
P G
PG
D000368:Aged; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D001249:Asthma / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D001706:Biopsy; D001980:Bronchi / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D001999:Bronchoscopy; D018801:CD2 Antigens; D017252:CD3 Complex / Q000032:analysis; D002277:Carcinoma / Q000473:pathology*; D002469:Cell Separation / Q000379:methods*; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D005069:Evaluation Studies as Topic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2; D015847:Interleukin-4 / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000032:analysis / Q000145:classification; D008175:Lung Neoplasms / Q000473:pathology*; D008875:Middle Aged; D009092:Mucous Membrane; D010835:Phytohemagglutinins; D011971:Receptors, Immunologic / Q000032:analysis; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D018801:CD2 Antigens; D017252:CD3 Complex; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011971:Receptors, Immunologic; D015847:Interleukin-4; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens