Differential expression of tomato (Lycopersicon esculentum L.) genes encoding shikimate pathway isoenzymes. I. 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.
Tomato (Lycopersicon esculentum L. cv. UC82b) was found to contain two distinct 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase genes that are differentially expressed. The corresponding cDNAs were isolated and characterized. Both genes code for putative plastidic DAHP synthase isoforms. The deduced amino acid sequences are 79% identical. A comparison of the known Solanaceae DAHP synthases indicates two distinct conserved isoforms. The steady-state levels of transcripts of the two tomato genes differ in all organs analysed.
Görlach
J
J
Institute of Plant Sciences, Swiss Federal Institute of Technology, Zürich.
Beck
A
A
Henstrand
J M
JM
Handa
A K
AK
Herrmann
K M
KM
Schmid
J
J
Amrhein
N
N
D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000235:genetics*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D003600:Cytosol / Q000201:enzymology; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D015723:Gene Library; D017343:Genes, Plant; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D010944:Plants / Q000033:anatomy & histology / Q000235:genetics*; D018087:Plastids / Q000201:enzymology; D011061:Poly A / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012765:Shikimic Acid / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D011061:Poly A; D012765:Shikimic Acid; D010723:3-Deoxy-7-Phosphoheptulonate Synthase