Cloning of a canine gene homologous to the human interleukin-8-encoding gene.
We cloned a 2.3-kb sequence homologous to the gene (hIL8) encoding human interleukin-8 (hIL-8) from the canine genome. The sequence may encode an 11.3-kDa protein consisting of 101 amino acids, sharing 75, 80 or 85% identity with the human, rabbit or porcine IL-8 proteins, respectively. Southern and Northern blot analyses indicated that the cloned sequence appeared to exist as a single copy in the canine genome, and that its expression was inducible with lipopolysaccharide in canine mononuclear cells, respectively. Thus, it seems very likely that the cloned gene is a canine homologue of hIL8.
Ishikawa
J
J
Department of Bioactive Molecules, National Institute of Health, Shinjuku, Tokyo, Japan.
Suzuki
S
S
Hotta
K
K
Hirota
Y
Y
Mizuno
S
S
Suzuki
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004285:Dogs / Q000235:genetics*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D016209:Interleukin-8; D012333:RNA, Messenger; D004247:DNA