Effects of vasodilating agents on cochlear blood flow in mice.
The mouse has several distinct advantages as an experimental model for study of the cochlear blood flow (CBF) in the mammal, particularly for studies involving genetic manipulation, development and aging factors. In this investigation we evaluated the effects of selected vasodilating agents on CBF using laser-Doppler flowmetry. Sodium nitroprusside, hydralazine and pentoxifylline were applied topically on the round window (RW) or systemically by a subcutaneous injection. Topical application of sodium nitroprusside and hydralazine elevated CBF significantly, while systemic administrations of these two drugs failed to elevate CBF consistently. Pentoxifylline did not change CBF following topical application but increased CBF slightly following systemic administration. Age and gender did not influence the CBF response to topical application of sodium nitroprusside.
Nakashima
T
T
Kresge Hearing Research Institute, University of Michigan, Ann Arbor 48109-0506.
Nuttall
A L
AL
Miller
J M
JM
D000287:Administration, Topical; D000818:Animals; D003051:Cochlea / Q000098:blood supply / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006830:Hydralazine / Q000008:administration & dosage / Q000494:pharmacology; D007279:Injections, Subcutaneous; D017078:Laser-Doppler Flowmetry; D008297:Male; D051379:Mice; D009599:Nitroprusside / Q000008:administration & dosage / Q000494:pharmacology; D010431:Pentoxifylline / Q000008:administration & dosage / Q000494:pharmacology; D012039:Regional Blood Flow / Q000187:drug effects; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000494:pharmacology*
D014665:Vasodilator Agents; D009599:Nitroprusside; D006830:Hydralazine; D010431:Pentoxifylline