Free fatty acids, neutral glycerides, and phosphoglycerides in transient focal cerebral ischemia.
Cerebral ischemia is known to cause an increase in levels of free fatty acids (FFAs) and diacylglycerols (DGs), although the mechanism(s) leading to these changes is not well understood. In this study, we examined FFA and DG levels along with those of other lipids in rats during and after transient focal cerebral ischemia induced by temporary occlusion of the right middle cerebral artery (MCA) and both common carotid arteries. During the duration of ischemia (15-60 min), there was a time-dependent increase (two- to 10-fold) in FFA levels in the right MCA cortex, whereas levels of DG and other lipids were not altered appreciably. FFA levels in right MCA cortex returned to near control values after reperfusion. However, following a 60-min ischemic insult, there was a second phase of FFA level increase that was evident after 16 h. The FFAs accumulated during the ischemia period were different from those after reperfusion, suggesting differences in mechanisms for their release. During the second phase of FFA release, there were increases in levels of DGs and triacylglycerols (TGs) with unusually high proportions of 20:4(n-6) and 22:6(n-3). The increases in FFA, DG, and TG levels were marked by a decrease in content of phosphoglycerides (PGs). It is interesting that the increases in levels of FFAs and neutral glycerides accounted only for 10% of the total PGs depleted. The lipid changes during this reperfusion period correlated well with the development of cortical infarct.(ABSTRACT TRUNCATED AT 250 WORDS)
Zhang
J P
JP
Biochemistry Department, University of Missouri, Columbia 65212.
Sun
G Y
GY
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005989:Glycerides / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism*; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
D004075:Diglycerides; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D005994:Glycerophosphates; D014280:Triglycerides