Genomic structure of the candidate proto-oncogene BCL3.
We previously reported the identification of a novel candidate proto-oncogene involved in the translocation t(14;19)(q32;q13) found in some cases of human B-cell chronic lymphocytic leukemia. This gene, BCL3, is a member of the I kappa B family, whose encoded proteins regulate the NF-kappa B family of transcription factors. Here we describe the genomic structure of BCL3. The gene contains nine exons, spanning 11.5 kb. In comparison to other members of the I kappa B family, there is a remarkable conservation of the exon-intron boundaries in relation to the coding sequences, consistent with an origin from a common ancestral gene. BCL3 is unusual in containing two CpG islands, a 5' island encompassing the first exon, the other lying within the gene. Southern blot analysis using methylation-sensitive restriction enzymes revealed that while the 5' CpG is unmethylated in all tissues tested, the degree of methylation of the internal CpG island varies.
McKeithan
T W
TW
Department of Pathology, University of Chicago, Illinois 60637.
Ohno
H
H
Dickstein
J
J
Hume
E
E
D000595:Amino Acid Sequence; D000081242:B-Cell Lymphoma 3 Protein; D001483:Base Sequence; D004276:DNA, Satellite; D015226:Dinucleoside Phosphates / Q000235:genetics; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D012091:Repetitive Sequences, Nucleic Acid; D014157:Transcription Factors
D000081242:B-Cell Lymphoma 3 Protein; D004276:DNA, Satellite; D015226:Dinucleoside Phosphates; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; C000631344:BCL3 protein, human; C000715470:MAS1 protein, human; C015772:CpG dinucleotide