[Effects of temperature and duration of storage of citrated blood on chemiluminescence of its phagocytes].
Relationship between chemiluminescent response of whole citrate capillary blood of normal subjects, patients with osteoarthrosis deformans (n = 21) and with systemic lupus erythematosus (n = 37) and the temperature and duration of storage of blood samples was under study. Luminol-dependent chemiluminescence was induced by barium sulfate microcrystals and recorded as a curve. Chemiluminescence intensity was found to increase if the samples were stored longer than 1 h, this increase being reliably higher in the patients with systemic lupus erythematosus than in normal subjects or patients with osteoarthrosis. Capacity of citrate blood phagocytes to chemiluminescent response is partially preserved after 24 h storage on the cold, but is commonly manifest only after an hour's adaptation to room temperature. The following phases are characteristic of the chemiluminescent response curve in the majority of cases: latent (up to 1.5 = 4 h), an abrupt rise, and slow decrease.
Mul'diiarov
P Ia
PIa
Volkova
Z I
ZI
D001793:Blood Preservation; D001800:Blood Specimen Collection; D002951:Citrates; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol; D008180:Lupus Erythematosus, Systemic / Q000097:blood; D010003:Osteoarthritis / Q000097:blood; D010586:Phagocytes; D013696:Temperature; D013997:Time Factors
D002951:Citrates; D008165:Luminol
Vliianie temperatury i srokov khraneniia tsitratnoĭ krovi na khemiliuminestsentsiiu ee fagotsitov.