Characterization of a promoter region supporting transcription of a novel human beta-galactoside alpha-2,6-sialyltransferase transcript in HepG2 cells.
In humans, two transcripts encoding beta-galactoside alpha-2,6-sialytransferase (EC 2.4.99.1.) have previously been described. One of the transcripts is widely expressed, whereas the other is restricted to mature B-cells. In this study we demonstrate the existence of a third transcript in the hepatoma cell-line HepG2. The expression of this transcript is controlled by a promoter region which efficiently supports transcription in HepG2 cells, and which harbours putative binding sites for liver-enriched and acute phase inducible transcription factors.
Aas-Eng
D A
DA
Department of Immunology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo.
Asheim
H C
HC
Deggerdal
A
A
Smeland
E
E
Funderud
S
S
D000210:Acute-Phase Reaction; D001483:Base Sequence; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D012799:Sialyltransferases / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D009363:Neoplasm Proteins; D014157:Transcription Factors; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase