title: A deletion hybrid breakpoint map of the chromosomal region 13q14-q21 orders 19 genetic markers in 10 intervals.
abstract: A deletion hybrid breakpoint map of the chromosomal region 13q14-q21 has been constructed using 19 DNA markers and 13 cell lines with breakpoints in this chromosomal region. The cell lines define 10 distinct intervals in this region, which spans approximately 20 Mb. The markers include 6 RFLP markers, 11 microsatellites that provisionally had been mapped to the region 13q14-21, and 2 new polymorphic CA-repeats that were developed from an EMBL3 library of cell line ICD, containing 13pter-q14.3. The following order of markers was established: CEN-D13S320-(D13S118, D13S153)-RB1-D13S319-D13S25-(D13S31, D13S59, D13S133, D13S137)-D13S163-D13S119-(D13S26, D13S55)-(D13S131, D13S134, D13S135, D13S144, D13S152)-TEL.
authors.affiliation: Department of Medical Genetics, University of Groningen, The Netherlands.
authors.forename: R F
authors.initials: RF
authors.lastname: Kooy
authors.forename: E
authors.initials: E
authors.lastname: Verlind
authors.forename: R H
authors.initials: RH
authors.lastname: Houwen
authors.forename: D N
authors.initials: DN
authors.lastname: Shapiro
authors.forename: L A
authors.initials: LA
authors.lastname: Hawthorn
authors.forename: J K
authors.initials: JK
authors.lastname: Cowell
authors.forename: H
authors.initials: H
authors.lastname: Scheffer
authors.forename: C H
authors.initials: CH
authors.lastname: Buys
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping / Q000379:methods*; D002882:Chromosomes, Human, Pair 13; D004276:DNA, Satellite / Q000235:genetics; D006527:Hepatolenticular Degeneration / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D017384:Sequence Deletion
chemical_list: D004276:DNA, Satellite