title: Cloning and characterization of centromeric DNA from Neurospora crassa.
abstract: The centromere locus from linkage group VII of Neurospora crassa has been cloned, characterized, and physically mapped. The centromeric DNA is contained within a 450-kb region that is recombination deficient, A+T-rich, and contains repetitive sequences. Repetitive sequences from within this region hybridize to a family of repeats located at or near centromeres in all seven linkage groups of N. crassa. Genomic Southern blots and sequence analysis of these repeats revealed a unique centromere structure containing a divergent family of centromere-specific repeats. The predominantly transitional differences between copies of the centromere-specific sequence repeats and their high A+T content suggest that their divergence was mediated by repeat-induced point (RIP) mutations.
authors.affiliation: Department of Biological Sciences, University of California, Santa Barbara 93106.
authors.forename: M
authors.initials: M
authors.lastname: Centola
authors.forename: J
authors.initials: J
authors.lastname: Carbon
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D015139:Blotting, Southern; D002503:Centromere / Q000737:chemistry / Q000502:physiology*; D016386:Chromosome Walking; D018244:Chromosomes, Artificial, Yeast; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular / Q000379:methods; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases; C068375:endodeoxyribonuclease PacI