Differentiation of an immortalized CNS neuronal cell line decreases their susceptibility to cytotoxic T cell lysis in vitro.
RN33B cells are a temperature-sensitive neuronal cell line derived from rat E12 medullary raphe nucleus (Whittemore and White (1993) Brain Research 615, 27-40). Undifferentiated RN33B cells express class I but not class II antigens of the major histocompatibility complex (MHC), and intercellular adhesion molecule-1 (ICAM-1), a ligand for lymphocyte function associated antigen-1 (LFA-1), expressed on cytotoxic T lymphocytes (CTLs). Treatment of undifferentiated RN33B cells with interferon-gamma (IFN-gamma) upregulated both class I MHC and ICAM-1. After neuronal differentiation, expression of class I MHC antigens or ICAM-1 was undetected, even after IFN-gamma treatment. The neuronally differentiated RN33B cells were also markedly less susceptible to lysis by alloantigen-specific CTLs. These data suggest that intrinsic to the differentiation of CNS neurons is a mechanism to escape CTL-mediated cell lysis.
White
L A
LA
Miami Project, University of Miami School of Medicine, FL 33136.
Keane
R W
RW
Whittemore
S R
SR
D000818:Animals; D001921:Brain / Q000166:cytology / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*
D015815:Cell Adhesion Molecules; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma