title: PCNA/cyclin and P-glycoprotein as prognostic factors in locally advanced breast cancer. An immunohistochemical, retrospective study.
abstract: OBJECTIVE
  The aim of the present study was to determine, retrospectively, whether the immunohistochemical expression of two biologic markers of aggressively, P-glycoprotein (P-gp) and PCNA/cyclin (PCNA), could be related to response to chemotherapy and prognosis in locally advanced breast cancer.
  
  
  METHODS
  PC 10 Mab was used to determine the proliferation index (PCNA) and C-219 Mab to determine P-gp in 25 locally advanced breast carcinomas subjected to preoperative chemotherapy with MDR-related drugs.
  
  
  RESULTS
  P-gp and PCNA were expressed in 76% and 100% of the tumors, respectively. No case of high P-gp expression was associated with good chemosensitivity, and all P-gp-negative cases showed the best chemotherapeutic response. P-gp and PCNA were both highly expressed in patients who developed local-regional or distant metastases. No recurrence was associated with a negative or low P-gp score.
  
  
  CONCLUSIONS
  Statistical analysis showed that high P-gp expression was related to a poor response to chemotherapy and a short disease-free survival. A high PCNA score was not found to be significant for predicting chemosensitivity or survival.
authors.affiliation: Istituto Nazionale dei Tumori, Fondazione G. Pascale, Napoli, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Botti
authors.forename: G
authors.initials: G
authors.lastname: Chiappetta
authors.forename: G
authors.initials: G
authors.lastname: D'Aiuto
authors.forename: E
authors.initials: E
authors.lastname: de Angelis
authors.forename: A
authors.initials: A
authors.lastname: De Matteis
authors.forename: M
authors.initials: M
authors.lastname: Montella
authors.forename: A
authors.initials: A
authors.lastname: Picone
authors.forename: F
authors.initials: F
authors.lastname: Cascione
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000328:Adult; D000951:Antigens, Neoplasm / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D001943:Breast Neoplasms / Q000737:chemistry* / Q000188:drug therapy / Q000401:mortality / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen; D012189:Retrospective Studies; D016019:Survival Analysis
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000951:Antigens, Neoplasm; D015415:Biomarkers; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen