Enantiomeric separation of drugs by affinity electrokinetic chromatography using dextran sulfate.
Dextran sulfate (sodium salt), which is a mixture of a linear alpha-(1,6)-linked D-glucose polymer having a sulfate group in the molecule, has been employed as a chiral selector in affinity electrokinetic chromatography (EKC) for the separation of enantiomers of drugs. Enantiomers of trimetoquinol hydrochloride and its positional isomer have been successfully separated by the method using a 3-5% dextran sulfate buffer solution of pH 5.5 within 20 min. The effects of buffer pH, concentration of dextran sulfate, and additives (such as an organic solvent, urea and a surfactant) on the enantioselectivity were investigated. The choice of pH and the concentration of dextran sulfate has been important for the improvement in selectivity.
Nishi
H
H
Analytical Chemistry Research Laboratory, Tanabe Seiyaku Company, Osaka, Japan.
Nakamura
K
K
Nakai
H
H
Sato
T
T
Terabe
S
S
D002021:Buffers; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D016264:Dextran Sulfate / Q000737:chemistry; D004563:Electrochemistry; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D013237:Stereoisomerism; D014213:Tretoquinol / Q000737:chemistry / Q000302:isolation & purification*
D002021:Buffers; D016264:Dextran Sulfate; D014213:Tretoquinol