BR140, a novel zinc-finger protein with homology to the TAF250 subunit of TFIID.
The Br140 protein was initially identified due to its co-purification with an integrin. We have cloned and sequenced the cDNA corresponding to the Br140 protein. Analysis of the amino acid sequence indicates that Br140 has features characteristic of gene regulatory proteins, including zinc fingers. Furthermore, Br140 has a homology to a family of transcriptional co-activators, one of which is a characterized component of the general transcriptional machinery. Immunologic studies indicate that Br140 has a broad tissue distribution and is abundant in testes and spermatogonia. Br140 is primarily a nuclear protein. These results suggest that Br140 may be a part of the general transcriptional machinery.
Thompson
K A
KA
Cancer Research Center, La Jolla Cancer Research Foundation, CA 92037.
Wang
B
B
Argraves
W S
WS
Giancotti
F G
FG
Schranck
D P
DP
Ruoslahti
E
E
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D017124:Conserved Sequence; D004268:DNA-Binding Proteins; D004730:Endothelium, Vascular / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D046911:Macromolecular Substances; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D011061:Poly A / Q000032:analysis / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D019474:Transcription Factors, TFIII; D014471:Umbilical Veins; D016335:Zinc Fingers; D000092468:Bromodomain Containing Proteins
D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D011061:Poly A; D012333:RNA, Messenger; D014157:Transcription Factors; D019474:Transcription Factors, TFIII; C086011:BRPF1 protein, human; D000092468:Bromodomain Containing Proteins; C053256:transcription factor TFIIID