Cloning and characterization of centromeric DNA from Neurospora crassa.
The centromere locus from linkage group VII of Neurospora crassa has been cloned, characterized, and physically mapped. The centromeric DNA is contained within a 450-kb region that is recombination deficient, A+T-rich, and contains repetitive sequences. Repetitive sequences from within this region hybridize to a family of repeats located at or near centromeres in all seven linkage groups of N. crassa. Genomic Southern blots and sequence analysis of these repeats revealed a unique centromere structure containing a divergent family of centromere-specific repeats. The predominantly transitional differences between copies of the centromere-specific sequence repeats and their high A+T content suggest that their divergence was mediated by repeat-induced point (RIP) mutations.
Centola
M
M
Department of Biological Sciences, University of California, Santa Barbara 93106.
Carbon
J
J
D001482:Base Composition; D001483:Base Sequence; D015139:Blotting, Southern; D002503:Centromere / Q000737:chemistry / Q000502:physiology*; D016386:Chromosome Walking; D018244:Chromosomes, Artificial, Yeast; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular / Q000379:methods; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases; C068375:endodeoxyribonuclease PacI