title: Peroxidase labeling of IgMs fragment of ABO blood group specific mouse monoclonal IgM.
abstract: A method for peroxidase labeling of the monomeric subunit (IgMs) of ABO blood group specific mouse monoclonal IgM is described. IgM was purified from a commercial monoclonal anti-B blood grouping reagent by a combination of salt precipitation, euglobulin precipitation, and gel filtration. IgM was mildly reduced with L-cysteine to yield SH-bearing IgMs. Finally, IgMs was conjugated to horseradish peroxidase, into which SH-reacting maleimide groups had been introduced using N-succinimidyl 6-maleimidohexanoate, through the selective reaction between SH of IgMs and maleimide groups of peroxidase.
authors.affiliation: Department of Legal Medicine, Miyazaki Medical College, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yukawa
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Seo
authors.forename: K
authors.initials: K
authors.lastname: Suetomi
authors.forename: K
authors.initials: K
authors.lastname: Takahama
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004355:Drug Stability; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting; D018796:Immunoconjugates / Q000737:chemistry / Q000302:isolation & purification; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000737:chemistry*; D066298:In Vitro Techniques; D008301:Maleimides / Q000737:chemistry; D051379:Mice; D011487:Protein Conformation
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D007075:Immunoglobulin M; D008301:Maleimides; C043592:maleimide; D006735:Horseradish Peroxidase