Determination of adenine nucleotides by fluorescence detection using high-performance liquid chromatography and post-column derivatisation with chloroacetaldehyde.
A novel rapid method for the analysis of adenine nucleotides in cells and tissues using post-column derivatisation with chloroacetaldehyde (CAA) followed by fluorescence detection is described. The CAA is incorporated in the eluent, but only reacts post-column when the temperature is elevated to 100 degrees C. Samples are chromatographed following neutralisation of acid extracts. Examples are given using both trichloroacetic acid extraction for cells in culture, and perchloric acid for murine liver.
Stratford
M R
MR
Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, Northwood, Middlesex, UK.
Dennis
M F
MF
D000079:Acetaldehyde / Q000031:analogs & derivatives; D000227:Adenine Nucleotides / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000737:chemistry; D051379:Mice; D010472:Perchlorates / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014238:Trichloroacetic Acid; D014407:Tumor Cells, Cultured
D000227:Adenine Nucleotides; D010472:Perchlorates; D014238:Trichloroacetic Acid; C004656:chloroacetaldehyde; D000079:Acetaldehyde