title: Antibodies to HMG proteins in patients with drug-induced autoimmunity.
abstract: OBJECTIVE
  To determine the prevalence of autoantibodies to high-mobility group (HMG) proteins in sera from patients with drug-induced lupus (DIL).
  
  
  METHODS
  Forty-two patients who developed autoantibodies and/or lupus after treatment with procainamide or other drugs were tested for HMG autoantibodies by immunoblotting and enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Twenty-eight of the 42 sera (67%) bound HMG-14 and/or HMG-17. In comparison, 9 of 42 (21%) bound HMG-1 and/or HMG-2. There was a good correlation between ELISA results and binding on immunoblots.
  
  
  CONCLUSIONS
  The high prevalence of antibodies to the nucleosomal core HMGs (HMG-14 and HMG-17) in DIL patients adds evidence implicating nucleosomes as immunogens in drug-induced autoimmunity.
authors.affiliation: Department of Medicine, University of Calgary, Alberta, Canada.
authors.forename: L M
authors.initials: LM
authors.lastname: Ayer
authors.forename: R L
authors.initials: RL
authors.lastname: Rubin
authors.forename: G H
authors.initials: GH
authors.lastname: Dixon
authors.forename: M J
authors.initials: MJ
authors.lastname: Fritzler
mesh_terms: D000070:Acebutolol / Q000627:therapeutic use; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000097:blood / Q000139:chemically induced; D015551:Autoimmunity / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006609:High Mobility Group Proteins / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008177:Lupus Vulgaris / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy; D011342:Procainamide / Q000009:adverse effects; D012460:Sulfasalazine / Q000627:therapeutic use
chemical_list: D001323:Autoantibodies; D006609:High Mobility Group Proteins; D012460:Sulfasalazine; D000070:Acebutolol; D011342:Procainamide