Therapeutic effect of intradermal injections with difucosyl lactosamine (dimeric Lex) on patients with rheumatoid arthritis.
As reported by us, a new myeloid cell population with an oncofetal membrane marker, dimeric Lex (di-Lex; III3FucV3 FucnLc6), was found in the epiphyseal bone marrow adjacent to the involved joints of patients with severe rheumatoid arthritis (RA). Patients with RA received intradermal (id) injections of di-Lex incorporated in liposome or of high molecular weight glycoprotein, or tumor associated carbohydrate antigen (TCA), containing the same carbohydrate epitope as di-Lex. The epiphyseal myeloid cells were reduced or sometimes eliminated during id injection. In random trials of id injection, observation under clinical and laboratory conditions showed improvement in 63% (17/27) of the patients treated for 6 months with appropriate doses of di-Lex (III3FucnLc4), and in 72% (31/43) of those treated with an identical protocol for TCA. However, id injection with monomeric Lex had no effect.
Ochi
T
T
Department of Orthopedic Surgery, Osaka University Medical School, Japan.
Hakomori
S
S
Fujimoto
M
M
Okamura
M
M
Owaki
H
H
Wakitani
S
S
Shimaoka
Y
Y
Hayashida
K
K
Tomita
T
T
Kawamura
S
S
D000328:Adult; D000368:Aged; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000008:administration & dosage; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000473:pathology; D001853:Bone Marrow / Q000473:pathology; D002240:Carbohydrate Sequence; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D005260:Female; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000008:administration & dosage; D006801:Humans; D007279:Injections, Subcutaneous; D016256:Lewis X Antigen / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use*; D008081:Liposomes; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D013997:Time Factors
D015295:Antigens, Tumor-Associated, Carbohydrate; D004337:Drug Carriers; D006023:Glycoproteins; D016256:Lewis X Antigen; D008081:Liposomes; C069719:sialosyl dimeric Le(x) antigen