Bauhinia purpurea lectin (BPA) binding of rat type I pneumocytes: alveolar epithelial alterations after radiation-induced lung injury.
In the rat lung, we found that the Bauhinia purpurea lectin (BPA) specifically binds to the type I alveolar epithelial cells and to the alveolar macrophages. Double label fluorescence employing FITC-coupled Maclura pomifera lectin (MPA) and bBPA-avidin-Texas Red showed that BPA binding was confined to type I cells. In addition, a minor staining of the luminal border of the terminal bronchiolar epithelium was found. The sequence of tissue injury following X-radiation was examined in rats. BPA is a suitable marker which indicates epithelial changes during the early stage of pneumonitis and the subsequent development of fibrosis.
Kasper
M
M
Department of Pathology, Technical University Dresden, Germany.
Schuh
D
D
Müller
M
M
D000818:Animals; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D004848:Epithelium / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D006651:Histocytochemistry; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D008168:Lung / Q000473:pathology / Q000528:radiation effects*; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D037121:Plant Lectins; D011650:Pulmonary Alveoli / Q000378:metabolism* / Q000473:pathology* / Q000528:radiation effects; D011658:Pulmonary Fibrosis / Q000378:metabolism / Q000473:pathology; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000473:pathology*; D017564:Radiation Pneumonitis / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344
C039340:Bauhinia purpurea lectin; D037102:Lectins; D037121:Plant Lectins; C034551:maclurin