Determination of L-glutamate and L-glutamine in pharmaceutical formulations by amperometric L-glutamate oxidase based enzyme sensors.
An amperometric biosensor for the direct determination of L-glutamate was developed by chemical bonding of L-glutamate oxidase (GAO) on a carboxylic Nylon membrane with polyazetidine prepolymer (PAP), and using a hydrogen peroxide electrode as indicating sensor. The biosensor is specific for L-glutamate and the peculiar analytical properties (linearity range, reproducibility, accuracy) were experimentally determined. Furthermore, the same basic biosensor was also modified to be used and characterized for the direct determination of L-glutamine. This L-glutamine biosensor was obtained by coimmobilizing, on two separate membranes, glutamic acid oxidase and glutaminase (GMN) on the same biosensor. The two sensors were then used for the determination of glutamate and L-glutamine contained in pharmaceutical formulations and the results were compared with those obtained by other analytical methods.
Botrè
F
F
Istituto di Merceologia, Rome, Italy.
Botrè
C
C
Lorenti
G
G
Mazzei
F
F
Porcelli
F
F
Scibona
G
G
D000594:Amino Acid Oxidoreductases / Q000737:chemistry*; D001384:Azetidines / Q000737:chemistry; D015374:Biosensing Techniques; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004800:Enzymes, Immobilized; D005971:Glutamates / Q000032:analysis*; D018698:Glutamic Acid; D005973:Glutamine / Q000032:analysis*; D008567:Membranes, Artificial; D009757:Nylons; D011108:Polymers; D015203:Reproducibility of Results; D013237:Stereoisomerism
D001384:Azetidines; D004800:Enzymes, Immobilized; D005971:Glutamates; D008567:Membranes, Artificial; D009757:Nylons; D011108:Polymers; D005973:Glutamine; C082735:azetidine; D018698:Glutamic Acid; D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase