title: Automated column liquid chromatographic determination of amoxicillin and cefadroxil in bovine serum and muscle tissue using on-line dialysis for sample preparation.
abstract: A fully automated method is described for the determination of amoxicillin and cefadroxil in bovine serum and muscle tissue. The method is based on the on-line combination of dialysis and solid-phase extraction for sample preparation, and column liquid chromatography with ultraviolet detection. In order to enhance the UV detectability of the analytes, post-column addition of 0.1 M sodium hydroxide is performed. The method shows good linearity and repeatability for both analytes in serum as well as in muscle tissue; the limits of detection in these samples are 0.05 microgram/ml and 0.2 microgram/g, respectively. The method has a sample throughput of 30 samples per 24 h.
authors.affiliation: Department of Analytical Chemistry, Free University, Amsterdam, Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: Snippe
authors.forename: N C
authors.initials: NC
authors.lastname: van de Merbel
authors.forename: F P
authors.initials: FP
authors.lastname: Ruiter
authors.forename: O M
authors.initials: OM
authors.lastname: Steijger
authors.forename: H
authors.initials: H
authors.lastname: Lingeman
authors.forename: U A
authors.initials: UA
authors.lastname: Brinkman
mesh_terms: D000658:Amoxicillin / Q000032:analysis* / Q000097:blood; D000818:Animals; D002417:Cattle; D002434:Cefadroxil / Q000032:analysis* / Q000097:blood; D002853:Chromatography, Liquid; D006863:Hydrogen-Ion Concentration; D008460:Meat / Q000032:analysis; D017551:Microdialysis; D009132:Muscles / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002434:Cefadroxil; D000658:Amoxicillin