title: Modulation of cell surface EGF receptor and HLA expression on glioma cell lines induced with cytokines and differentiation promoters.
abstract: Alterations of cell surface expression of HLA (class I, class II DR, DP and DQ) and EGF-receptor on two malignant glioma cell lines (U-343MG and U-563MG) induced with cytokines (IFN-gamma, TNF-alpha, IL-1 alpha) and differentiation promoters (all-trans retinoic acid, phorbol ester TPA) were analyzed with the aid of flow cytometry. IFN-gamma induced a 10-15fold increase of HLA class I. TNF-alpha alone induced a two- to fivefold increase of HLA class I cell surface density and increased the IFN-gamma induced upregulation of HLA class I to approximately 20-24 times the antigen density of uninduced cells. TNF-alpha was able to increase HLA class II DR and DP cell surface expression on glioma lines, but it enhanced only the IFN-gamma-induced HLA class II DR upregulation. All-trans retinoic acid and TPA regulated in the opposite way the EGF-receptor cell surface expression on U-563MG cells.
authors.affiliation: Cancer Research Institute, Bratislava, Slovak Republic.
authors.forename: J
authors.initials: J
authors.lastname: Sedlák
authors.forename: L
authors.initials: L
authors.lastname: Hunáková
authors.forename: B
authors.initials: B
authors.lastname: Chorváth
authors.forename: M
authors.initials: M
authors.lastname: Grófová
mesh_terms: D000954:Antigens, Surface / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology*; D066246:ErbB Receptors / Q000187:drug effects* / Q000502:physiology*; D005910:Glioma / Q000737:chemistry* / Q000276:immunology*; D006680:HLA Antigens / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000954:Antigens, Surface; D016207:Cytokines; D006680:HLA Antigens; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D014212:Tretinoin; D007371:Interferon-gamma; D066246:ErbB Receptors; D013755:Tetradecanoylphorbol Acetate