Histamine H2 receptors and histidine decarboxylase in normal and leukemic human monocytes and macrophages.
Spontaneous and all-trans-retinoic acid (RA)-induced differentiation of normal human monocytes and of leukemic THP-1 monocytes into macrophages resulted in a progressive loss of adenosine 3',5'-cyclic monophosphate production induced by histamine via typical H2 receptors (H2R). In THP-1 cells and in HL-60 human acute myelocytic leukemia cells, RA treatment increased the abundance of the 4.5-kb messenger RNA of the H2R gene fourfold, suggesting transcriptional control by a RA response element. Scatchard plots of [3H]tiotidine binding indicated the expression of H2R with similar affinity and binding capacity in THP-1 monocytes and macrophages, while the conversion of normal monocytes into macrophages decreased H2R density from 91.8 to 43.1 fmol/mg protein, with no change in affinity (Kd = 9.9 to 11.2 nM). In THP-1 macrophages, histamine inhibited 4 beta-phorbol 12-myristate 13-acetate (PMA)-induced H2O2 formation via the activation of H2 receptors. Expression of the H2R gene, histamine accumulation, and histidine decarboxylase activity were also demonstrated in normal human monocytes/macrophages and peripheral lymphocytes. Histamine and H2R may therefore affect, via intracrine, autocrine, and paracrine pathways, various immune and inflammatory responses of the lymphoid and myeloid progenitors and lineages in the bone marrow and peripheral tissues.
Mirossay
L
L
Institut National de la Santé et de la Recherche Médicale U55, Hôpital Saint-Antoine, Paris, France.
Chastre
E
E
Callebert
J
J
Launay
J M
JM
Housset
B
B
Zimber
A
A
Abita
J P
JP
Gespach
C
C
D000262:Adenylyl Cyclases / Q000378:metabolism; D001665:Binding Sites; D002927:Cimetidine / Q000031:analogs & derivatives / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis; D004789:Enzyme Activation; D015870:Gene Expression; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000378:metabolism; D006640:Histidine Decarboxylase / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D008264:Macrophages / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D011969:Receptors, Histamine H1 / Q000235:genetics / Q000378:metabolism*; D012016:Reference Values; D014407:Tumor Cells, Cultured
D006635:Histamine H2 Antagonists; D011969:Receptors, Histamine H1; D002927:Cimetidine; D006632:Histamine; D006861:Hydrogen Peroxide; D000242:Cyclic AMP; D006640:Histidine Decarboxylase; D000262:Adenylyl Cyclases; C023990:tiotidine