Cloning and preliminary characterization of two satellite-like DNA sequences from the brown frog Rana graeca.
Two repetitive DNA sequences of about 0.29 Kb and 0.39 Kb have been isolated from Rana graeca genomic DNA by digestion with Asp718I and have been cloned in pTZ18R. Hybridization data obtained with DNA probes derived from these clones indicate that: i) both sequences are highly repetitive and species-specific; ii) the two sequences are partially homologous; iii) the 0.29 Kb sequence is present in the frog genome with the typical tandem organization of satellite DNA; iv) the 0.39 Kb sequence is presumably part of a longer repetitive sequence of satellite DNA.
Rosati
C
C
Istituto di Strutture Biologiche ed Ultrastruttura Cellulare, Facoltà di Medicina e Chirurgia, Università Federico II, Napoli.
Cardone
D
D
Feliciello
I
I
Chinali
G
G
D000818:Animals; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics* / Q000302:isolation & purification; D011898:Ranidae
D004276:DNA, Satellite