title: Use of proton nmr spectroscopy to measure propofol metabolites in the urine of the female Caucasian patient.
abstract: 1. Six female Caucasian patients received i.v. doses of propofol (2.7 +/- 0.3 (SE) mg/kg) for induction of anaesthesia. Anaesthesia was maintained with nitrous oxide, oxygen and enflurane for periods up to 3 h. A total urine collection was made from each subject for 24 h after administration of propofol; samples were concentrated and analysed for propofol metabolites by nmr spectroscopy. 2. By 24 h, 50.9 +/- 4.0% of the dose of propofol was recovered as metabolites. The proportions of propofol metabolites, 1-quinol glucuronide (1-QG), 4-quinol glucuronide (4-QG), propofol glucuronide (PG) and 4-quinol sulphate (4-QS) recovered in urine (0-24 h) of the patients were 12 +/- 1, 8 +/- 2, 76 +/- 4 and 4 +/- 1% respectively. However, one patient showed a metabolite profile in which PG comprised 93% and 1-QG 7% of the total metabolites. 3. Nmr spectroscopy has been shown to be a satisfactory method for the quantification of propofol metabolites in urine without the necessity for reference samples.
authors.affiliation: Department of Anaesthesia, University Hospital of South Manchester Withington Hospital, West Didsbury, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: Sneyd
authors.forename: P J
authors.initials: PJ
authors.lastname: Simons
authors.forename: B
authors.initials: B
authors.lastname: Wright
mesh_terms: D000328:Adult; D000758:Anesthesia; D005260:Female; D005965:Glucuronates / Q000652:urine; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008875:Middle Aged; D015742:Propofol / Q000008:administration & dosage / Q000652:urine*; D013431:Sulfates / Q000652:urine
chemical_list: D005965:Glucuronates; D013431:Sulfates; D015742:Propofol