A Zea mays GTP-binding protein of the ARF family complements an Escherichia coli mutant with a temperature-sensitive malonyl-coenzyme A:acyl carrier protein transacylase.
In an attempt to isolate a plant malonyl-coenzyme A:acyl carrier protein transacylase cDNA clone, by direct genetic selection in an Escherichia coli fabD mutant (LA2-89) with a maize cDNA expression library, a Zea mays cDNA clone encoding a GTP-binding protein of the ARF family was isolated. Complementation of a mutation affecting bacterial membrane lipid biosynthesis by a plant ARF protein, could indicate the existence of as yet unidentified bacterial equivalents of this ubiquitous eucaryotic GTP-binding protein.
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Department of Genetics, BioCentrum Amsterdam, Vrije Universiteit, Netherlands.
Brown
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Slabas
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Stuitje
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AR
D020727:ADP-Ribosylation Factors; D051080:Acyl-Carrier Protein S-Malonyltransferase; D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D054889:Fatty Acid Synthase, Type II; D019204:GTP-Binding Proteins / Q000235:genetics*; D005816:Genetic Complementation Test / Q000379:methods*; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D003313:Zea mays / Q000235:genetics*
D018076:DNA, Complementary; D029968:Escherichia coli Proteins; D000217:Acyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase; C494968:fabD protein, E coli; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors; D054889:Fatty Acid Synthase, Type II