title: Distinct effects of thrombospondin-1 and CISP/thrombospondin-2 on adrenocortical cell spreading.
abstract: Corticotropin-induced secreted protein (CISP) is a trimeric protein secreted by bovine adrenocortical cells in response to ACTH, that is likely to represent the bovine form of thrombospondin-2 (TSP2). This study was aimed at delineating the respective effects of CISP/TSP2 and TSP1 (thrombospondin-1) on adrenocortical cell attachment and spreading. TSP1 and CISP/TSP2 were found to slightly reduce the attachment of adrenocortical cells to plastic in the presence of serum but exhibited a pronounced differential effect on cell spreading. CISP/TSP2 inhibited adrenocortical cell spreading in a dose-dependent manner (maximal effect with 40 micrograms/ml) whereas TSP1 (up to 100 micrograms/ml) did not influence this process. The inhibition of spreading was observed whether plates were coated with CISP/TSP2 alone or with a mixture of CISP/TSP2 and fibronectin. We suggest that the inhibition of in vitro adrenocortical cell spreading by CISP/TSP2 is indicative of an implication of this protein in the migration of adrenocortical cells in vivo.
authors.affiliation: INSERM Unité 244, CEA, Département de Biologie Moléculaire et Structurale, Centre d'Etudes Nucléaires, Grenoble, France.
authors.forename: S
authors.initials: S
authors.lastname: Pellerin
authors.forename: B
authors.initials: B
authors.lastname: Lafeuillade
authors.forename: E M
authors.initials: EM
authors.lastname: Chambaz
authors.forename: J J
authors.initials: JJ
authors.lastname: Feige
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D001769:Blood; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D003470:Culture Media; D005353:Fibronectins / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D010969:Plastics; D019699:Thrombospondins
chemical_list: D002135:Calcium-Binding Proteins; D015815:Cell Adhesion Molecules; D003470:Culture Media; D005353:Fibronectins; D008562:Membrane Glycoproteins; D010969:Plastics; D019699:Thrombospondins; C079864:corticotropin-induced secreted protein, Bos taurus