Changes in biliary (high-molecular-mass) and liver isoforms of alkaline phosphatase after baboon-to-human liver transplantation.
We report a case of hyperphosphatasemia in a 35-year-old patient with hepatitis B who underwent an orthotopic xenogeneic liver transplant. Marked increases in total alkaline phosphatase (ALP; EC 3.1.3.1) activity began 5 days posttransplantation (six times human normal) and increased to approximately 17 times normal at day 11. Increased ALP persisted for > 40 days and steadily increased to 75 times normal in the patient's last 30 days. Gel electrophoresis detected both liver (LALP) and biliary (high-molecular-mass, BALP) isoforms. LALP measured with ion-exchange columns revealed an activity time course pattern similar to that of total ALP. Results for BALP activity also obtained with ion-exchange columns exhibited broad variability, ranging from 2 to 428 times normal.
Mercer
D
D
Department of Pathology, University of Pittsburgh Medical Center, PA 15213.
Tang
M
M
Marino
I R
IR
Demetris
A
A
Fung
J
J
Starzl
T
T
Warty
V
V
D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001646:Bile / Q000201:enzymology*; D001663:Bilirubin / Q000097:blood; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D016031:Liver Transplantation; D008297:Male; D010215:Papio; D010710:Phosphates / Q000097:blood; D012016:Reference Values; D014183:Transplantation, Heterologous; D005723:gamma-Glutamyltransferase / Q000097:blood
D007527:Isoenzymes; D010710:Phosphates; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin