Co-storage in large 'dense-core' vesicles of dopamine and cholecystokinin in rat striatum.
The subcellular localization of cholecystokinin in the striatum--an area where a high density of cholecystokinin containing terminals has been demonstrated--was studied using biochemical techniques. Cholecystokinin containing vesicles were partially purified using iso-osmotic Ficoll gradients. As judged from their size and their buoyant density in isopycnic gradients, cholecystokinin containing vesicles represent large 'dense-core' vesicles. Negative staining and subsequent immunolabelling for synaptophysin at the electron microscopical level, showed labelled vesicles of 50-70 nm. binding of dihydrotetrabenazine was detected in the cholecystokinin containing fractions. The results suggest that dopamine is co-stored with cholecystokinin in large dense vesicles in rat striatum.
Llona
I
I
Department of Medicine, University of Antwerp (UIA), Belgium.
Annaert
W G
WG
Jacob
W
W
De Potter
W P
WP
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002766:Cholecystokinin / Q000378:metabolism* / Q000502:physiology; D003342:Corpus Striatum / Q000378:metabolism* / Q000502:physiology; D004298:Dopamine / Q000378:metabolism* / Q000502:physiology; D005362:Ficoll; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D013395:Sucrose
D000906:Antibodies; D005362:Ficoll; D013395:Sucrose; D002766:Cholecystokinin; D004298:Dopamine