Cloning and expression of a glutamate transporter from mouse brain.
A complementary DNA clone encoding a transporter for glutamate has been isolated from mouse brain, and its functional properties have been examined in Xenopus oocytes. The transporter displays high affinity for glutamate (Km = 72 microM) and is dependent on external Na+. The cDNA sequence predicts a protein of 543 amino acids with 6 potential transmembrane domains. Northern blot analysis indicates the mRNA encoding the glutamate transporter is expressed both in the brain and in peripheral tissues.
Tanaka
K
K
Laboratory for Neural Networks, Institute of Physical and Chemical Research, RIKEN, Saitama, Japan.
D000595:Amino Acid Sequence; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000502:physiology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006023:Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012964:Sodium / Q000502:physiology; D014981:Xenopus
D027322:Amino Acid Transport System X-AG; D018076:DNA, Complementary; D005971:Glutamates; D006023:Glycoproteins; D012333:RNA, Messenger; D018698:Glutamic Acid; D012964:Sodium; D012321:DNA-Directed RNA Polymerases