Effect of diflubenzuron on incorporation of [3H]-N-acetylglucosamine ([3H]NAGA) into chitin in the intact integument from the newly molted American cockroach Periplaneta americana.
1. Diflubenzuron and polyoxin D clearly inhibited the incorporation of [3H]-N-acetylglucosamine ([3H]NAGA) into chitin in the isolated integument from newly molted American cockroaches under the experimental condition. 2. Upon homogenization, or tissue slicing, such an inhibitory effect of diflubenzuron on chitin synthesis totally disappeared, while that of polyoxin D did not. 3. Mitochondrial poisons (oligomycin and FCCP), protein phosphorylation modulations (8-Br-cAMP, Na3VO4 and MnCl2), potassium ionophores (valinomycin), and calmodulin inhibitor (trifluoperazine) clearly inhibited [3H]NAGA incorporation. 4. Phospholipase A2 and calcium ion significantly enhanced the [3H]NAGA incorporation.
Nakagawa
Y
Y
Department of Environmental Toxicology, University of California, Davis 95616.
Matsumura
F
F
Hashino
Y
Y
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002686:Chitin / Q000096:biosynthesis*; D004132:Diflubenzuron / Q000494:pharmacology*; D008563:Membrane Lipids / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010526:Periplaneta / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011741:Pyrimidine Nucleosides / Q000494:pharmacology; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000378:metabolism
D000900:Anti-Bacterial Agents; D008563:Membrane Lipids; D010743:Phospholipids; D011480:Protease Inhibitors; D047428:Protein Kinase Inhibitors; D011500:Protein Synthesis Inhibitors; D011741:Pyrimidine Nucleosides; D014763:Viral Matrix Proteins; D002686:Chitin; D014537:Uridine Diphosphate N-Acetylglucosamine; C002458:polyoxorim; D011494:Protein Kinases; D004132:Diflubenzuron