Polyclonals to beta-amyloid(1-42) identify most plaque and vascular deposits in Alzheimer cortex, but not striatum.
Alzheimer's beta-peptides (A beta) aggregation rates depend on A beta length. We made synthetic peptide antisera to A beta 34-40 and 37-42. Purified anti-34-40 preferentially recognizes beta 1-40, vascular amyloid and a subset of plaques while purified anti-37-42 recognizes A beta 1-42 and not A beta 1-40 in dot and Western blots and immunoprecipitates; 37-42 precipitates a small percentage of fibroblast secreted A beta and strongly stains all deposits identified by monoclonals to A beta on adjacent sections from frontal cortex, but not in dorsal striatum.
Mak
K
K
Department of Medicine, UCLA, USA.
Yang
F
F
Vinters
H V
HV
Frautschy
S A
SA
Cole
G M
GM
D000544:Alzheimer Disease / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000032:analysis*; D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D006224:Cricetinae; D005625:Frontal Lobe / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D017072:Neostriatum / Q000473:pathology*
D016229:Amyloid beta-Peptides; D000906:Antibodies