title: Chemiluminescence and immunoassays.
abstract: The principles of chemiluminescence and the application of chemiluminescent labels and substrates in immunoassays are reviewed. Various immunoassay formats and all known classes of chemiluminescent molecules, including 1,2-dioxetanes, luminol and derivatives, acridinium esters, oxalate esters and firefly luciferins are described as well as the many sensitizers and fluorescent enhancers. Recent promising developments are discussed.
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Utrecht University, The Netherlands.
authors.forename: H A
authors.initials: HA
authors.lastname: Rongen
authors.forename: R M
authors.initials: RM
authors.lastname: Hoetelmans
authors.forename: A
authors.initials: A
authors.lastname: Bult
authors.forename: W P
authors.initials: WP
authors.lastname: van Bennekom
mesh_terms: D000818:Animals; D006801:Humans; D007118:Immunoassay; D008163:Luminescent Measurements