title: [Characteristics of Pseudomonas fluorescens lipopolysaccharide].
abstract: A lipopolysaccharide was isolated from Pseudomonas fluorescens IMV 1152 (biovar I). The fractions of the structural moieties of lipopolysaccharide macromolecule were extracted and studied separately. 3-hydroxydecanoic, 3-hydroxydodecanoic and 2-hydroxydodecanoic fatty acids were identified in lipid A, total quantity of those was more than 80%. In acid hydrolysate of lipid A the glucosamine and phosphoethanolamine were found. The rhamnose, arabinose, glucose, glucosamine and phosphorus were identified as the components of core oligosaccharide fractions. A trisaccharide repeating unit of the O-cpecific polysaccharide chain consists of the residues of aminosugars: 4-acet-amido-4,6-dideoxy-D-galactose, 2-acetamido-2,6-dideoxy-D-glucose and 2-acetamido-2,6-dideoxy-L-glucose. The studied lipopolysaccharide resembled the structure of lipid A and core oligosaccharide with lipopolysaccharide from type strain of Pseudomonas fluorescens IMV 4125 (ATCC 13525), whereas their O-chains were different.
authors.forename: S N
authors.initials: SN
authors.lastname: Veremeĭchenko
authors.forename: G M
authors.initials: GM
authors.lastname: Zdorovenko
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011551:Pseudomonas fluorescens / Q000737:chemistry*
chemical_list: D008070:Lipopolysaccharides
vernacular_title: Kharakteristika lipopolisakharida Pseudomonas fluorescens.