Involvement of human leukocyte antigen class I molecules in human immunodeficiency virus infection of CD4-positive cells.
We have studied the putative roles of human immunodeficiency virus (HIV)-associated and cell surface-expressed major histocompatibility complex class I (MHC-I) molecules in the course of the HIV life cycle by the combined use of MHC-I molecule-positive and MHC-I molecule-negative virus particles and MHC-I molecule-positive and MHC-I molecule-negative CD4+ human cells. We found (i) that several anti-MHC-I monoclonal antibodies neutralize cell infection by direct interaction with HIV-associated MHC-I antigens, (ii) that these HIV-associated MHC-I antigens are however dispensable for cell infection, and (iii) that the cell surface-expressed MHC-I molecules are unnecessary for productive infection of CD4+ human cells. These results clarify further the functions of MHC-I molecules during the HIV life cycle.
Benkirane
M
M
Centre de Tri des Molécules anti-HIV, Institut National de la Santé et de la Recherche Médicale U249, Montpellier, France.
Blanc-Zouaoui
D
D
Hirn
M
M
Devaux
C
C
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000382:microbiology*; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D015497:HIV-1 / Q000276:immunology / Q000502:physiology*; D015498:HIV-2 / Q000276:immunology / Q000502:physiology*; D006684:HLA-DR Antigens / Q000276:immunology / Q000502:physiology; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D009500:Neutralization Tests; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000187:drug effects / Q000276:immunology
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I