Cloning and nucleotide sequence of the gene coding for the major 25-kilodalton outer membrane protein of Brucella abortus.
The cloning and sequencing of the Brucella abortus major 25-kDa outer membrane protein (OMP) is reported. The 25-kDa (group 3) OMP has been proposed, on the basis of amino acid composition, to be the counterpart of OmpA (D. R. Verstraete, M. T. Creasy, N. T. Caveney, C. L. Baldwin, M. W. Blab, and A. J. Winter, Infect. Immun. 35:979-989, 1982). However, the amino acid sequence predicted from the cloned B. abortus gene did not reveal significant homology with either OmpA sequences from different members of the family Enterobacteriaceae or other known protein sequences.
de Wergifosse
P
P
Unit of Experimental Medicine, Catholic University of Louvain, Brussels, Belgium.
Lintermans
P
P
Limet
J N
JN
Cloeckaert
A
A
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002003:Brucella abortus / Q000737:chemistry*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment
D001425:Bacterial Outer Membrane Proteins