[Chromosome fractionation in a saccharose density gradient for subsequent flow sorting].
The human chromosomes were obtained from lymphoblastoid cell line BOLD with normal karyotype, and separated on the basis of their size by velocity sedimentation at 190 g in sucrose gradient. Different chromosomal fractions were stained by applying fluorochromes with different DNA-base specificity: Hoechst 33258 and Olivomycine and were analysed using dual laser cell-sorter ATC-3000 (Brucker). Velocity sedimentation allowed to enrich certain fractions by individual chromosomes and thus speed up the sorting rate from 90 up to 200 chromosomes per second.
Shatrova
A N
AN
Aksenov
N D
ND
Teslenko
L V
LV
Zenin
V V
VV
D002458:Cell Fractionation / Q000379:methods*; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods; D002877:Chromosomes, Human / Q000648:ultrastructure*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007834:Lasers; D008677:Metaphase
Fraktsionirovanie khromosom v gradiente plotnosti sakharozy dlia posleduiushcheĭ protochnoĭ sortirovki.