Sequence of a 2.6-kb cryptic plasmid from a marine cyanobacterium Synechococcus sp.
We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb EcoRI fragment. This ORF encodes a putative protein which is 360 aa residues in length and is 37.8% homologous to the replication protein of plasmid pCA2.4 from Synechocystis sp. strain PCC 6803, 35.8% homologous to an ORF from the Nostoc plasmid pGL2, and 33.2% homologous to the ORF of a plasmid from Lactobacillus plantarum, pC30il. Highly conserved regions of amino acid sequence were also found between ORF-B and other bacterial plasmids.
Kawaguchi
R
R
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Nagaoka
T
T
Burgess
J G
JG
Takeyama
H
H
Matsunaga
T
T
D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014170:Transformation, Genetic
D004269:DNA, Bacterial