A rapid and simple method for the purification of transglutaminase from Streptoverticillium mobaraense.
Transglutaminase from Streptoverticillium mobaraense was partially purified by ion-exchange chromatography on a weak acid material and hydrophobic chromatography. The separation with a strong acid ion-exchanger produces homogeneous transglutaminase, in a single step and with high yields, directly from the centrifuged and filtered culture fluid of the micro-organism. The procedure reproduced several times could be also carried out on a larger scale with the optimized parameters of the laboratory isolations. The purified enzyme demonstrated good storage stability.
Gerber
U
U
Fachbereich Chemische Technologie, Fachhochschule Darmstadt, Germany.
Jucknischke
U
U
Putzien
S
S
Fuchsbauer
H L
HL
D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D013306:Streptomycetaceae / Q000201:enzymology*; D011503:Transglutaminases / Q000302:isolation & purification*
D011503:Transglutaminases