title: Nucleotide sequence of the coat protein gene of the Skierniewice isolate of plum pox virus (PPV).
abstract: The coat protein (CP) gene of the Skierniewice isolate of plum pox virus (PPV-S) has been amplified using the reverse transcription--polymerase chain reaction (RT-PCR), cloned and sequenced. The nucleotide sequence of the gene and the deduced amino-acid sequence of PPV-S CP were compared with those of other PPV strains. The nucleotide sequence showed very high homology to most of the published sequences. The motif: Asp-Ala-Gly (DAG), important for the aphid transmissibility, was present in the amino-acid sequence. Our isolate did not react in ELISA with monoclonal antibodies MAb06 supposed to be specific for PPV-D.
authors.affiliation: Department of Biopolymer Biochemistry, A. Mickiewicz University, Poznań, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Wypijewski
authors.forename: T
authors.initials: T
authors.lastname: Malinowski
authors.forename: W
authors.initials: W
authors.lastname: Musiał
authors.forename: J
authors.initials: J
authors.lastname: Augustyniak
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D005814:Genes, Viral; D015335:Molecular Probes; D008969:Molecular Sequence Data; D017801:Plum Pox Virus / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D015335:Molecular Probes