[The interaction of bifunctional monoclonal antibodies with antigens studied by a radioimmunological method].
The quadroma produced bifunctional antibodies (bAbs) with double specificity to alpha-endorphin (alpha-END) and horseradish peroxidase (HRP) were compared with the parental anti-alpha-END monoclonal antibodies (mAbs) in respect to their binding to alpha-END. bAbs were purified from quadroma culture medium by sequential HRP-sepharose and alpha-END-sepharose affinity chromatography. Using radioimmunological method the affinity of the individual anti-alpha-END combining sites of bAbs was shown to be identical to that of parental mAbs. Binding to the second antigen (HRP) didn't affect binding of bAbs to alpha-END.
Dergunova
N N
NN
Massino
Iu S
IuS
Kizim
E A
EA
Dmitriev
A D
AD
D000818:Animals; D018033:Antibodies, Bispecific / Q000032:analysis / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000378:metabolism*; D000915:Antibody Affinity; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D004723:Endorphins / Q000276:immunology; D006735:Horseradish Peroxidase / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D011863:Radioimmunoassay; D018822:alpha-Endorphin
D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal; D004723:Endorphins; D018822:alpha-Endorphin; D006735:Horseradish Peroxidase
Izuchenie vzaimodeĭstviia bifunktsional'nykh monoklonal'nykh antitel s antigenami radioimmunologicheskim metodom.