The O-chain of Brucella abortus lipopolysaccharide induces SDS-resistant MHC class II molecules in mouse B cells.
LPS is the most important antigen of Brucella bacteria which are gram-negative facultative intracellular pathogens infecting a large proportion of animals and humans in the world. In order to get insights into the immune response mechanisms monitored by Brucella, its LPS was used as a model antigen. S-LPS, R-LPS, lipid A and O-chain purified from Brucella abortus were tested in their capacity of inducing SDS-resistant MHC class II molecules after incubation with murine B lymphoma cells. S-LPS and O-chain gave a significant response suggesting that O-chain might induce an association with class II itself or might act as a carrier for antigens to bind MHC class II molecules.
Escola
J M
JM
Centre d'Immunologie de Marseille-Luminy, France.
Moreno
E
E
Chavrier
P
P
Gorvel
J P
JP
D000818:Animals; D002003:Brucella abortus / Q000737:chemistry*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002645:Chickens; D004351:Drug Resistance; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D006358:Hot Temperature; D007163:Immunosorbent Techniques; D008050:Lipid A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology*; D016393:Lymphoma, B-Cell / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D009113:Muramidase / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014407:Tumor Cells, Cultured
D000949:Histocompatibility Antigens Class II; D008050:Lipid A; D008070:Lipopolysaccharides; D012967:Sodium Dodecyl Sulfate; D009113:Muramidase