In vivo monitoring of norepinephrine and hydroxyl free radical generation by ferrous iron in the myocardium with a microdialysis technique.
1. We examined in vivo monitoring of norepinephrine and hydroxyl radical generation in rat myocardium with a microdialysis technique. For this purpose, we designed the microdialysis probe holding system which includes loose fixation of the tube and synchronization of the movement of the heart and the probe. 2. The hydroxyl free radical (.OH) reacts with salicylate and generates 2,3- and 2,5-dihydroxybenzoic acid (DHBA) which can be measured electrochemically in picomole quantity by high performance liquid chromatography (HPLC). 3. After probe implantation, norepinephrine concentration of dialysate decreased over the first 150 min and then reached an almost steady level. A positive linear correlation between the ferrous iron and .OH formation trapped as 2,3-DHBA (R2 = 0.960) and 2,5-DHBA (R2 = 0.982) was observed using the microdialysis technique. 4. The present results indicate that non-enzymatic oxidation in the extracellular fluid may play a key role in hydroxyl radical generation by ferrous iron.
Obata
T
T
Department of Pharmacology, Oita Medical University, Japan.
Hosokawa
H
H
Yamanaka
Y
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000494:pharmacology*; D005841:Gentisates; D062385:Hydroxybenzoates / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000494:pharmacology; D008297:Male; D017551:Microdialysis; D009206:Myocardium / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
D005296:Ferrous Compounds; D005841:Gentisates; D062385:Hydroxybenzoates; D007502:Iron Chelating Agents; D017665:Hydroxyl Radical; C009135:2,3-dihydroxybenzoic acid; C010925:2,5-dihydroxybenzoic acid; D009638:Norepinephrine