title: Two chronic myelogenous leukaemia cell lines which represent different stages of erythroid differentiation.
abstract: We established two cell lines, YN-1 and Y-1K, from the peripheral blood of two chronic myelogenous leukaemia patients in blastic crisis. Characterization of the YN-1 and Y-1K cells revealed that these cells expressed erythroid lineage markers. However, there was a marked difference in the level of gamma-globin mRNA and haemoglobin in YN-1 and Y-1K cells. YN-1 contained approximately 1-5% benzidine-positive staining cells, whereas no benzidine-positive cells were observed in Y-1K cells. Haemoglobin production in YN-1 cells was markedly increased with various chemical inducers of erythroid differentiation, but was not in Y-1K cells. In contrast, Y-1K cells expressed CD34 stem cell antigen and CD41 megakaryocyte-specific antigen. These observations suggested that, although both cell lines were committed to the erythroid lineage, each cell line represented a distinct differentiation stage in the erythroid differentiation programme. Y-1K seemed to correspond to an early stage of cells in erythroid lineage, whereas YN-1 represented a more advanced stage in human erythropoiesis.
authors.affiliation: Second Department of Internal Medicine, Tohoku University, Sendai, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Endo
authors.forename: H
authors.initials: H
authors.lastname: Harigae
authors.forename: T
authors.initials: T
authors.lastname: Nagai
authors.forename: H
authors.initials: H
authors.lastname: Fujie
authors.forename: K
authors.initials: K
authors.lastname: Meguro
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Furuyama
authors.forename: J
authors.initials: J
authors.lastname: Kameoka
authors.forename: M
authors.initials: M
authors.lastname: Okuda
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D004305:Dose-Response Relationship, Drug; D004900:Erythroblasts / Q000648:ultrastructure; D015672:Erythroid Precursor Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015321:Gene Rearrangement; D005914:Globins / Q000032:analysis; D006427:Hemin / Q000378:metabolism; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D006454:Hemoglobins; D012333:RNA, Messenger; D006427:Hemin; D005914:Globins