Aflatoxin M1 in human breast milk samples from Victoria, Australia and Thailand.
This study examined the exposure of infants to aflatoxin M1 (AFM1) and of lactating mothers to aflatoxin B1 (AFB1), using AFM1 in breast milk as a biomarker for exposure to AFB1. An enzyme-linked immunosorbent assay was modified for the analysis of AFM1 in breast milk samples from 73 women from Victoria for comparison with breast milk samples from Thailand (n = 11). The results were compared with those obtained by HPLC. AFM1 was detected in 11 samples from Victoria and five samples from Thailand at median concentrations of 0.071 ng/ml (range 0.028 to 1.031 ng/ml) and 0.664 ng/ml (range 0.039 to 1.736 ng/ml), respectively. Levels of AFM1 in Thai milk samples were significantly higher than those in milk samples from Victoria.
el-Nezami
H S
HS
RMIT University, Key Centre for Applied and Nutritional Toxicology, Melbourne, Victoria, Australia.
Nicoletti
G
G
Neal
G E
GE
Donohue
D C
DC
Ahokas
J T
JT
D016604:Aflatoxin B1 / Q000009:adverse effects* / Q000378:metabolism; D016607:Aflatoxin M1 / Q000032:analysis* / Q000378:metabolism; D001667:Binding, Competitive; D015415:Biomarkers / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007774:Lactation; D016014:Linear Models; D008895:Milk, Human / Q000737:chemistry* / Q000378:metabolism; D010047:Ovalbumin / Q000378:metabolism; D010865:Pilot Projects; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013785:Thailand; D014739:Victoria
D015415:Biomarkers; D016607:Aflatoxin M1; D010047:Ovalbumin; D016604:Aflatoxin B1