Mycoplasma pulmonis 46-kDa trypsin-resistant protein adheres to rat tracheal epithelial cells.
Results of competitive binding studies with radiolabeled and unlabeled Mycoplasma pulmonis and rat tracheal explant cultures indicated no effect of trypsin treatment on the ability of M. pulmonis to bind to explants. A trypsin-resistant 46-kDa membrane protein, which binds isolated rat tracheal epithelial cells in culture, was purified and used to produce specific antibodies that block adhesion of mycoplasmas to tracheal explants. These results suggest that M. pulmonis adheres to rat tracheal epithelium via a trypsin-resistant 46-kDa protein.
Beyers
T M
TM
Department of Pathology, University of Texas Southwestern Medical Center at Dallas 75235-9037.
Lai
W C
WC
Read
R W
RW
Pakes
S P
SP
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D001667:Binding, Competitive; D004848:Epithelium / Q000382:microbiology; D008297:Male; D008970:Molecular Weight; D009174:Mycoplasma / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D014132:Trachea / Q000382:microbiology*; D014357:Trypsin / Q000494:pharmacology
D000906:Antibodies; D001426:Bacterial Proteins; D014357:Trypsin