title: Hypoxic inhibition of breathing in fetal sheep: relationship to brain adenosine concentrations.
abstract: Because hypoxic inhibition of fetal breathing may be caused by a rise in central adenosine levels, the effects of O2 deficiency on fetal brain adenosine concentrations were determined at levels of hypoxia that inhibited fetal breathing. Under halothane anesthesia, the brains of fetal sheep (0.8 term) were implanted with guide cannulas exteriorized through a Silastic rubber window in the uterus and flank of the ewe. At least 4 days after surgery, a microdialysis probe was inserted into a cannula with the membrane tip placed in the rostral brain stem. During 1 h of isocapnic hypoxia, mean fetal arterial PO2 was reduced from 24.0 +/- 0.9 Torr (control) to 13 +/- 0.6 Torr and arterial pH fell progressively from 7.354 +/- 0.007 to 7.273 +/- 0.023. Hypoxia decreased the incidence of fetal breathing movements from 33 +/- 5.2 to 5 +/- 2.2 min/h, with a normal incidence (29 +/- 3.5 min/h) during the hour after arterial PO2 returned to control values. Adenosine concentrations in microdialysis perfusate under control conditions averaged approximately 35 nM, increased up to 2.3-fold during the hour of O2 deficiency, and fell toward control values when normoxia was restored. We conclude that fetal brain adenosine levels are increased at levels of O2 deficiency that inhibit fetal breathing, which are results consistent with a role for adenosine in hypoxic inhibition of fetal breathing.
authors.affiliation: Nicholas S. Assali Perinatal Research Laboratory, Department of Obstetrics and Gynecology, University of California, Los Angeles School of Medicine 90024.
authors.forename: B J
authors.initials: BJ
authors.lastname: Koos
authors.forename: B A
authors.initials: BA
authors.lastname: Mason
authors.forename: O
authors.initials: O
authors.lastname: Punla
authors.forename: A M
authors.initials: AM
authors.lastname: Adinolfi
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D001158:Arteries; D001921:Brain / Q000196:embryology*; D002319:Cardiovascular System / Q000503:physiopathology; D005260:Female; D005324:Fetal Movement; D005333:Fetus / Q000502:physiology*; D005740:Gases / Q000097:blood; D000860:Hypoxia / Q000503:physiopathology*; D009994:Osmolar Concentration; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D012119:Respiration; D012756:Sheep
chemical_list: D005740:Gases; D000241:Adenosine; D010100:Oxygen