Semi-quantitative in situ hybridization and immunohistology for antigen expression of human herpesvirus-6 in various lymphoproliferative diseases.
Fifty patients with various hyperplastic and malignant lymphoproliferative diseases were investigated for evidence of human herpesvirus-6 infection. Virus DNA and antigen expression was investigated in lymph node biopsies by in situ hybridization and immunohistology and was correlated with data of immunophenotyping. Supplemental immunoglobulin- and T cell receptor gene rearrangement studies were used to support the classification of the proliferative lymphoid lesion. Elevated numbers of cells carrying HHV-6 DNA and/or antigens were found in cases of Hodgkin's lymphoma and follicular center cell non-Hodgkin's lymphoma as well as atypical polyclonal lymphoproliferation (APL), yet not in reactive lymphoid hyperplasia and in most other lymphomas. Immunophenotyping showed that virus -infected cells were primarily lympho-histiocytic elements, less frequently Hodgkin's- and Reed-Sternberg cells, and not malignant B lymphocytes as in follicular center cell lymphomas. This suggests that the virus is rather not the causative oncogen in these cases, yet does not exclude a cocarcinogenic effect of it during the development and ths course of uncontrolled lymphoproliferation.
Rojo
J
J
Banco de Sangre, Hospital General de Mexico, Universidad National Autonoma, D.F. Mexico.
Ferrer Argote
V E
VE
Klueppelberg
U
U
Krueger
G R
GR
Eidt
E
E
Ablashi
D V
DV
Luka
J
J
Tesch
H
H
D000956:Antigens, Viral / Q000032:analysis*; D003043:Cocarcinogenesis; D004279:DNA, Viral / Q000032:analysis; D006566:Herpesviridae Infections / Q000235:genetics / Q000473:pathology; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000235:genetics / Q000473:pathology / Q000821:virology; D006801:Humans; D006965:Hyperplasia; D016130:Immunophenotyping; D017403:In Situ Hybridization; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000473:pathology / Q000821:virology; D008232:Lymphoproliferative Disorders / Q000235:genetics / Q000473:pathology / Q000821:virology*; D009363:Neoplasm Proteins / Q000032:analysis; D011230:Precancerous Conditions / Q000473:pathology / Q000821:virology
D000956:Antigens, Viral; D004279:DNA, Viral; D009363:Neoplasm Proteins