The effect of propionate on lipid synthesis in rat lymphocytes.
1. The effect of propionate on lipid synthesis in lymphocytes cultured for 24 hr and incubated for 2 hr was investigated. 2. [1-14C]-propionate was incorporated mainly into phospholipids in both control and concanavalin A (Con A) stimulated cultured lymphocytes. 3. The content of free coenzyme A markedly decreased in 2 hr incubated lymphocytes when propionate was added to the medium at concentrations from 10 to 100 mmol/l. 4. Propionate at 40 mmol/l decreased the incorporation of [1-14C]-palmitate into phospholipids (86%), triacylglycerol (87%) and cholesterol ester (98%) and increased in cholesterol (133%) of cultured lymphocytes. 5. Addition of propionate into the culture medium at 2.5 and 5.0 mmol/l concentrations markedly increased the activity of hydrolases of various acylCoA derivatives. 6. The results suggest that propionate may reduce the content of acylCoA and so its esterification and this might be important for the regulation of lymphocytes proliferation.
Curi
R
R
Department of Physiology and Biophysics, São Paulo University, Butantan, Brazil.
Rosa
L F
LF
Yano
M
M
Bond
J A
JA
Homem de Bittencourt
P I
PI
Newsholme
E A
EA
D000818:Animals; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D003065:Coenzyme A / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000502:physiology; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D010168:Palmitates / Q000378:metabolism; D011422:Propionates / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D002250:Carbon Radioisotopes; D007657:Ketone Bodies; D008055:Lipids; D010168:Palmitates; D011422:Propionates; D006867:Hydrolases; D003065:Coenzyme A