Hepatic metabolism of spironolactone. Production of 3-hydroxy-thiomethyl metabolites.
Spironolactone (SP) is used clinically as a renal aldosterone antagonist and as an antiandrogen. It is known that the drug is extensively metabolized and that metabolites mediate its therapeutic actions, but hepatic metabolism of SP has not been comprehensively investigated. Hepatic disposition may also be important in the toxicity of SP, because the parent compound prevents the hepatocarcinogenic effects of its metabolite, canrenone (CAN). Using a combination of in vivo and in vitro approaches, we studied the metabolism of SP by guinea pig livers. The major compounds detected in livers in vivo following SP treatment were the known metabolites, 7 alpha-thiomethyl-spirolactone (TM) and CAN, and a previously uncharacterized compound whose mass spectral and UV absorption characteristics suggested that it was an A-ring-reduced derivative of TM. In vitro incubation of liver homogenates with SP also resulted in the formation of the unknown metabolite. A combination of MS and NMR spectroscopy was used to identify unequivocally the unknown metabolites as 3 alpha-hydroxy-TM. Another metabolite produced in vitro was identified as 3 beta-hydroxy-TM. It is possible that these two new metabolites of SP contribute to the pharmacological actions of the drug. In addition, production of 3 alpha-hydroxy-TM suggests a mechanism to account for the prevention of CAN-induced carcinogenicity by SP. TM may block the conversion of CAN to mutagenic 3-hydroxy-CAN metabolites by serving as a competitive substrate for hepatic 3-keto reductases.
Los
L E
LE
Department of Pharmacology and Toxicology, Philadelphia College of Pharmacy and Science, PA 19104.
Pitzenberger
S M
SM
Ramjit
H G
HG
Coddington
A B
AB
Colby
H D
HD
D000818:Animals; D002192:Canrenone / Q000378:metabolism; D006168:Guinea Pigs; D008099:Liver / Q000378:metabolism*; D008297:Male; D013148:Spironolactone / Q000378:metabolism*; D013237:Stereoisomerism
D013148:Spironolactone; D002192:Canrenone