Role of adhesion glycoproteins CD18 and intercellular adhesion molecule-1 in complement-mediated reactions of rabbit skin.
1. The role of the adhesion glycoproteins CD18 and intercellular adhesion molecule-1 (ICAM-1) in inflammatory responses produced during a reversed passive Arthus (RPA) reaction and induced by zymosan and zymosan-activated plasma (ZAP) were studied in rabbit skin. 2. Oedema formation and haemorrhage were quantified by measuring accumulation of 125I-albumin and 111In-labelled red blood cells (111In-RBC) respectively. 3. Monoclonal antibody (mAb) R15.7 (anti-CD18), administered intravenously, abolished accumulation of 125I-albumin and 111In-RBC in dermal RPA reactions and in response to locally injected zymosan and ZAP. 4. When administered intravenously, the mAb RR1/1 (anti-ICAM-1) suppressed 125I-albumin and 111In-RBC accumulation in dermal RPA reactions and at sites treated with zymosan and ZAP. 5. Oedema formation in response to platelet-activating factor (PAF) and bradykinin (BK) either in the presence or absence of prostaglandin E2 (PGE2) were not affected by mAb R15.7 or by mAb RR1/1.1.1. 6. We conclude that oedema formation and haemorrhage associated with RPA reactions and in responses to zymosan and ZAP are completely CD18-dependent, and are mediated, at least in part, via ICAM-1. Responses to the neutrophil-independent oedema forming mediators, PAF and BK are not dependent upon CD18 or ICAM-1.
Norman
K E
KE
Department of Applied Pharmacology, National Heart & Lung Institute, London.
Argenbright
L W
LW
Williams
T J
TJ
Rossi
A G
AG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D001183:Arthus Reaction / Q000276:immunology*; D001920:Bradykinin / Q000494:pharmacology; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules / Q000276:immunology*; D015232:Dinoprostone / Q000494:pharmacology; D004285:Dogs; D004487:Edema / Q000209:etiology; D006470:Hemorrhage / Q000209:etiology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D051379:Mice; D010972:Platelet Activating Factor / Q000494:pharmacology; D011817:Rabbits; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology; D012867:Skin / Q000276:immunology*; D015054:Zymosan / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D010972:Platelet Activating Factor; D016028:Receptors, Leukocyte-Adhesion; D018799:Intercellular Adhesion Molecule-1; D015054:Zymosan; D015232:Dinoprostone; D001920:Bradykinin