Characterization of the gene encoding the glutamic-acid-specific protease of Streptomyces griseus.
The complete gene sequence (sprE) for the glutamic-acid-specific serine protease (SGPE) of the gram-positive bacterium Streptomyces griseus is reported. The sprE gene encodes a 355 amino acid pre-pro-mature enzyme. The presence of a glutamic acid residue at the junction of the pro and mature segments of the protein suggests that the enzyme is self-processing. SGPE was found to have extensive homology with the S. griseus proteases A and B. However, there is an additional segment to the pro region of SGPE, lacking in proteases A and B, which has significant homology to the pro region of the alpha-lytic protease of the gram-negative bacterium Lysobacter enzymogenes. Expression of recombinant SGPE in Bacillus subtilis is also reported, and the enzyme is shown to be self-processing.
Sidhu
S S
SS
Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada.
Kalmar
G B
GB
Borgford
T J
TJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012697:Serine Endopeptidases / Q000235:genetics*; D013305:Streptomyces griseus / Q000201:enzymology* / Q000235:genetics; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D005971:Glutamates; D011994:Recombinant Proteins; D018698:Glutamic Acid; D012697:Serine Endopeptidases; C069136:glutamyl endopeptidase II