title: Differentiation of vaccine and wild-type polioviruses using polymerase chain reaction and restriction enzyme analysis.
abstract: Genomic amplification followed by selective digestion of restriction enzymes was used to differentiate polioviruses. The method was based on conserved and variable components of the 5'-noncoding region. The differences between Sabin vaccine and wild-type viruses made it possible to identify rapidly an isolated poliovirus as vaccine-related or wild-type virus. A total of 60 isolates and strains were tested and all of them were correctly identified. This method is recommended as a sensitive, specific and rapid way to differentiate polioviruses in clinical isolates and environmental samples.
authors.affiliation: Robert Koch Institute of the Federal Health Office, Berlin, Federal Republic of Germany.
authors.forename: B
authors.initials: B
authors.lastname: Schweiger
authors.forename: E
authors.initials: E
authors.lastname: Schreier
authors.forename: B
authors.initials: B
authors.lastname: Böthig
authors.forename: J M
authors.initials: JM
authors.lastname: López-Pila
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D004279:DNA, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D017955:Poliovirus / Q000302:isolation & purification*; D011054:Poliovirus Vaccine, Inactivated / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral; D011054:Poliovirus Vaccine, Inactivated