A late exclusion of bacteriophage T4 can be suppressed by Escherichia coli GroEL or Rho.
A litCon mutation in Escherichia coli TU6 results in exclusion of bacteriophage T4 during the late, morphogenetic stage of its development at low temperatures. DNA was synthesized continuously in the infected cells, but less than 10% of the DNA made by 90 min after infection was packaged into DNAase-resistant particles, few viable phage were formed, and the cells lysed poorly. The exclusion could be relieved by conditions leading to elevated levels, determined immunologically, of the E. coli Rho protein (believed to be involved in regulation of T4 transcription), or chromosomally encoded E. coli GroEL (a chaperone known to be involved in phage assembly), or by supplying GroEL in trans from a plasmid. The two suppressing proteins appeared to act independently of each other. GroEL-suppression restored packaging to normal levels, perhaps by preventing GP23 from activating the host Lit protein; in addition DNA synthesis was delayed and reduced and cell lysis enhanced, demonstrating involvement of GroEL in both these processes. Rho suppression was less efficient. Since both transcription-termination-proficient and transcription-termination-deficient Rho suppressed, the results raise the possibility that Rho has a role during T4 development not directly involving transcription regulation.
Linder
C H
CH
Department of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Uppsala, Sweden.
Carlson
K
K
Albertioni
F
F
Söderström
J
J
Påhlson
C
C
D001426:Bacterial Proteins / Q000502:physiology*; D001433:Bacteriolysis / Q000235:genetics; D017122:Bacteriophage T4 / Q000254:growth & development*; D002213:Capsid / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D036022:Capsid Proteins; D018834:Chaperonin 60; D018833:Chaperonins; D004279:DNA, Viral / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000502:physiology*; D009154:Mutation; D011506:Proteins / Q000502:physiology; D012234:Rho Factor / Q000502:physiology*; D013489:Suppression, Genetic; D014161:Transduction, Genetic
D001426:Bacterial Proteins; D036022:Capsid Proteins; D018834:Chaperonin 60; D004279:DNA, Viral; D006360:Heat-Shock Proteins; D011506:Proteins; D012234:Rho Factor; C064517:gp23 protein, Bacteriophage T4; D018833:Chaperonins