The human T cell antigen receptor repertoire: skewed use of V beta gene families by CD8+ T cells.
The TCR repertoire of human CD8+ peripheral blood lymphocytes has been determined using MoAbs to the V beta 2, 3, 5.1, 5.2/5.3, 6.7, 8, 12 and 19(17)V beta gene families. The CD8T cell repertoire for V beta 2 and V beta 3 is shown to be skewed, with an excess of individuals having higher values than are consistent with a normal distribution. A significant majority of these individuals are over the age of 40. High values of V beta CD8+ cells were found for each V beta family studied except for 6.7a. Individual high values are stable for at least 12 months. In addition, the total percentage of CD4 and CD8 cells reacting with this panel of reagents was determined. There is a significant excess of V beta + CD4+ cells (33%) over CD8+V beta + cells (21.9%). Thus the human CD8 V beta repertoire differs from the human CD4 repertoire in a number of important ways.
Clarke
G R
GR
Department of Clinical Medicine, University of Leeds, St. James's University Hospital, UK.
Humphrey
C A
CA
Lancaster
F C
FC
Boylston
A W
AW
D000328:Adult; D000375:Aging / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D005810:Multigene Family; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis*; D013223:Statistics as Topic; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D016827:CD8 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta