Effects of thyroid hormones on chick embryo muscle cell culture.
The importance of thyroid hormones (TH) in the normal development of muscles has been repeatedly postulated. The effects of physiological TH doses on ornithine decarboxylase (ODC) and protein synthesis in muscle cells have been studied using cell cultures prepared from 11-day-old chick embryos. Triiodothyronine nuclear receptors in primary muscle cell culture were characterized on the basis of the specific binding analysis as a single receptor class with the equilibrium dissociation constant Kd = 1.2 +/- 0.4 x 10(-10) mol/l and binding capacity Bmax = 0.21 +/- 0.09 fmol/micrograms DNA. While the physiological amounts of both triiodothyronine (T3) and thyroxine (T4) stimulated ODC activity after 2 hr of treatment, only T3 had the same stimulatory effect after 4 hr of treatment. Twenty-four hour exposure of muscle cell culture to TH did not change ODC activity. The incorporation of [3H]leucine into proteins was elevated only after 120 hr incubation in the presence of T4. Application of T4 caused also an increase in the protein content after 24 hr.
Výboh
P
P
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji.
Lamosová
D
D
Vaneková
M
M
Juráni
M
M
D000818:Animals; D001667:Binding, Competitive; D002478:Cells, Cultured; D002642:Chick Embryo; D007553:Isotope Labeling; D007930:Leucine / Q000378:metabolism; D009124:Muscle Proteins / Q000096:biosynthesis; D009132:Muscles / Q000166:cytology / Q000187:drug effects* / Q000196:embryology / Q000201:enzymology; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011988:Receptors, Thyroid Hormone / Q000378:metabolism; D013974:Thyroxine / Q000378:metabolism / Q000494:pharmacology*; D014284:Triiodothyronine / Q000378:metabolism / Q000494:pharmacology*
D009124:Muscle Proteins; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D009955:Ornithine Decarboxylase; D007930:Leucine; D013974:Thyroxine