Detection of HIV-1 DNA in leukocytes using a commercially available assay.
Recently, an assay for detection of proviral HIV-1 DNA in leukocytes became commercially available. This assay (Amplicor HIV-1 test, Roche Diagnostic Systems) multiplies HIV-1DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV-1-infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false-positive results, we advise to repeat initially reactive samples if no other data confirm HIV-infection.
Zaaijer
H L
HL
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Cuypers
H T
HT
Reesink
H W
HW
ten Veen
J H
JH
Schoon-Visser
S C
SC
Lelie
P N
PN
D001782:Blood Donors; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D005189:False Positive Reactions; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000737:chemistry / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic