Humoral response in rabbits immunized with calcium phosphate adjuvanted HIV-1 gp160 antigen.
Rabbits were immunized with either calcium phosphate adjuvanted purified HIV-1 gp160 or a fluid preparation. Circulating antibodies were detected by ELISA, RIPA and Western Blot tests. Sera of rabbits immunized with the adjuvanted preparation contained high levels of anti-gp160 antibodies, as well as antibodies recognizing p55, p25 and p18. On the contrary, rabbits immunized with the fluid preparation contained only anti-p18 antibodies. Neutralizing antibodies were also detected. It is concluded that the calcium phosphate adjuvant could be used for preparation of candidate anti-HIV vaccines, since it permits one to induce high levels of circulating antibodies, in the absence of untoward reactions as observed when aluminium adjuvants or water in oil emulsions are used.
Relyveld
E
E
Institut Pasteur, Unité des Vaccins Bactériens, Marnes-La-Coquette, France.
Chermann
J C
JC
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D015153:Blotting, Western; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000494:pharmacology*; D017024:Chemotherapy, Adjuvant; D004797:Enzyme-Linked Immunosorbent Assay; D015686:Gene Products, env / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology*; D019302:HIV Envelope Protein gp160; D007114:Immunization; D008297:Male; D011498:Protein Precursors / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology*; D011817:Rabbits
D002130:Calcium Phosphates; D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors