Suspension culture of Catharanthus roseus crown gall cell induced by Agrobacterium C58.
In comparison with calli from leaf or stem, Catharanthus roseus crown gall cell cultured on MS basic medium was superior in growth, total indole alkaloids and ajmalicine contents. The effects of illumination, cultural temperature, sucrose level of the medium and exogenous L-Trp on the growth, total indole alkaloids and ajmalicine contents of C. roseus crown gall cell cultures were studied. The results will provide a theoretical basis for the attempt of using suspension cultures of C. roseus crown gall cells to produce indole alkaloids.
Wang
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Biochemistry and Molecular Biology Department of Nankai University, Tianjing, China.
Wang
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S
Tian
J
J
Li
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X
Zhu
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L
D000470:Alkaloids / Q000302:isolation & purification*; D002478:Cells, Cultured; D003470:Culture Media; D007211:Indoles / Q000302:isolation & purification*; D007218:Industrial Microbiology; D008027:Light; D010941:Plant Tumors; D012231:Rhizobium; D046948:Secologanin Tryptamine Alkaloids; D013696:Temperature; D015016:Yohimbine / Q000031:analogs & derivatives* / Q000302:isolation & purification
D000470:Alkaloids; D003470:Culture Media; D007211:Indoles; D046948:Secologanin Tryptamine Alkaloids; D015016:Yohimbine; C005709:raubasine