title: A role of heat shock proteins for homologous recombination in Escherichia coli.
abstract: Effects of mutations in heat shock protein genes on homologous recombination in Escherichia coli were examined by measuring intra-molecular recombinations in the plasmid, pIK43. Recombination frequencies in the groES, groEL and dnaK mutants were decreased 6 to 21% of those of the isogenic wild type strain, at a sub-lethal temperature. Thus, the heat shock proteins GroES, GroEL and DnaK apparently participate in homologous recombination in Escherichia coli.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ogata
authors.forename: T
authors.initials: T
authors.lastname: Miki
authors.forename: K
authors.initials: K
authors.lastname: Sekimizu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D018835:Chaperonin 10; D018834:Chaperonin 60; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D017930:Genes, Reporter; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*; D012808:Sigma Factor / Q000235:genetics; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32; C063842:dnaK protein, E coli