Crystallization of the reaction center from Rhodobacter sphaeroides in a new tetragonal form.
The reaction center from the nonsulfur purple bacterium Rhodobacter sphaeroides has been crystallized in a new form. The crystals grew in the presence of polyethylene glycol 4000, the detergent beta-octyl glucoside, and the amphiphiles heptane triol and benzamidine hydrochloride, using the sitting drop method. The space group of these crystals is tetragonal, P4(1)(4(3))2(1)2, and the cell constants area a = b = 141.5 A and c = 276.7 A with probably 2 proteins per asymmetric unit. A native data set has been set collected to a resolution of 2.8 A consisting of 56,332 unique reflections (50,731 with F > 2 sigma) with an Rsym of 9.5%. Analysis of the diffraction data is underway using molecular and isomorphous replacement.
Allen
J P
JP
Department of Chemistry and Biochemistry, Arizona State University, Tempe 85287-1604.
D001550:Benzamidines; D003460:Crystallization; D018360:Crystallography, X-Ray; D005233:Fatty Alcohols; D005960:Glucosides; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D011092:Polyethylene Glycols; D011487:Protein Conformation; D012242:Rhodobacter sphaeroides / Q000737:chemistry*
D001550:Benzamidines; D005233:Fatty Alcohols; D005960:Glucosides; D045322:Photosynthetic Reaction Center Complex Proteins; C068115:heptane-1,2,3-triol; C018619:octyl-beta-D-glucoside; D011092:Polyethylene Glycols; C032157:benzamidine