title: Detection of HIV-1 DNA in leukocytes using a commercially available assay.
abstract: Recently, an assay for detection of proviral HIV-1 DNA in leukocytes became commercially available. This assay (Amplicor HIV-1 test, Roche Diagnostic Systems) multiplies HIV-1DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV-1-infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false-positive results, we advise to repeat initially reactive samples if no other data confirm HIV-infection.
authors.affiliation: Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: H L
authors.initials: HL
authors.lastname: Zaaijer
authors.forename: H T
authors.initials: HT
authors.lastname: Cuypers
authors.forename: H W
authors.initials: HW
authors.lastname: Reesink
authors.forename: J H
authors.initials: JH
authors.lastname: ten Veen
authors.forename: S C
authors.initials: SC
authors.lastname: Schoon-Visser
authors.forename: P N
authors.initials: PN
authors.lastname: Lelie
mesh_terms: D001782:Blood Donors; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D005189:False Positive Reactions; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000737:chemistry / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic