title: In vitro isolation and cultivation of a Babesia from an American woodland caribou (Rangifer tarandus caribou).
abstract: A Babesia species isolated from a captive caribou (Rangifer tarandus caribou) with clinical signs of babesiosis and a circulating parasitemia was cultured in vitro. Normal adult caribou erythrocytes supported the growth of the Babesia sp., as did erythrocytes from white-tailed deer (Odocoileus virginianus). Two basal media (M-199 and RPMI-1640) and a defined medium (HL-1) each supplemented with adult bovine serum were compared. The most favorable growth of the parasite occurred in HL-1 medium with 20% adult bovine serum. The morphology of this Babesia sp. isolate shared some characteristics with B. odocoilei and B. divergens.
authors.affiliation: Department of Veterinary Pathobiology, Texas A&M University, College Station 77843.
authors.forename: P J
authors.initials: PJ
authors.lastname: Holman
authors.forename: K
authors.initials: K
authors.lastname: Petrini
authors.forename: J
authors.initials: J
authors.lastname: Rhyan
authors.forename: G G
authors.initials: GG
authors.lastname: Wagner
mesh_terms: D000818:Animals; D001403:Babesia / Q000254:growth & development / Q000302:isolation & purification*; D001404:Babesiosis / Q000097:blood / Q000469:parasitology*; D002417:Cattle; D015925:Cryopreservation / Q000662:veterinary; D003470:Culture Media; D003670:Deer / Q000097:blood; D004912:Erythrocytes / Q000469:parasitology; D008297:Male; D012053:Reindeer / Q000469:parasitology*
chemical_list: D003470:Culture Media