Characterization of chromosome 9 deletions in transitional cell carcinoma by microsatellite assay.
A panel of 18 superficial or invasive transitional cell carcinomas (TCCs) was analyzed for chromosome 9 deletions by performing a high-density loss of heterozygosity (LOH) analysis. Twenty-five microsatellite loci were assayed by the polymerase chain reaction (PCR) and 7 restriction fragment length polymorphism (RFLP) loci were analyzed by Southern blotting. Concordant results were obtained with these methods, including direct comparisons at 2 loci. Chromosome 9 LOH was observed in 13 (72%) of 18 informative cases, including 10 superficial lesions. In contrast, LOH on chromosomes 10, 15, 20 and 21 was < or = 8%. Evidence for missing copies of chromosome 9 was observed in 7 of 13 LOH cases. Comparison of cases with subchromosomal LOH implicated the region between the D9S55 locus on 9p12 and the argininosuccinate synthetase (ASS) locus on 9q34.1 as the location of a tumor suppressor gene relevant to TCC.
Linnenbach
A J
AJ
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Pressler
L B
LB
Seng
B A
BA
Kimmel
B S
BS
Tomaszewski
J E
JE
Malkowicz
S B
SB
D001124:Argininosuccinate Synthase / Q000235:genetics; D001483:Base Sequence; D002295:Carcinoma, Transitional Cell / Q000235:genetics*; D002872:Chromosome Deletion; D002899:Chromosomes, Human, Pair 9; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
D017931:DNA Primers; D004273:DNA, Neoplasm; D004276:DNA, Satellite; D005819:Genetic Markers; D001124:Argininosuccinate Synthase