Variability occurs in the inverted repeat region of genomic DNA from bovine herpesvirus 1 respiratory, genital and bovine herpesvirus 5 encephalitic isolates.
Restriction fragment length polymorphisms (RFLPs) were detected within BHV1.1, BHV1.2, and BHV5 genomes using the restriction enzyme PstI. The genomic areas of these changes has not been previously reported. Using Southern blot hybridization with DNA probes representing the entire genome of BHV1.1, areas of genomic variation were located for a respiratory isolate (BHV1.1), four vaccine isolates (BHV1.1), a genital isolate (BHV1.2), and two encephalitic isolates (BHV5). The most frequently observed RFLPs of BHV1.1 and BHV1.2 occurred within the internal repeat region and the left terminus of the unique long region. When two separate isolates of the encephalitic BHV5 were compared, RFLPs were detected in the internal and right terminal repeat regions. These are the regions of each genome from which immediate early genes are transcribed. No genomic variation was observed throughout the unique long and unique short regions for all BHV1 and 5 isolates examined.
Whetstone
C A
CA
Virology Cattle Research, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010.
Seal
B S
BS
Miller
J M
JM
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D015342:DNA Probes; D004279:DNA, Viral / Q000737:chemistry; D004660:Encephalitis / Q000382:microbiology / Q000662:veterinary; D005260:Female; D014644:Genetic Variation; D005831:Genital Diseases, Female / Q000382:microbiology / Q000662:veterinary; D016679:Genome, Viral; D006564:Herpesviridae / Q000235:genetics*; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D007241:Infectious Bovine Rhinotracheitis / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes; D004279:DNA, Viral