Interaction of toxic trace metals and mechanisms of detoxification in the planktonic diatoms Ditylum brightwellii and Thalassiosira pseudonana.
Effects of cadmium (10 nM), copper (80 nM) and zinc (150 nM) additions were studied in the marine diatom Ditylum brightwellii and the riverine diatom Thalassiosira pseudonana. Defense against oxidative stress via cellular thiol (SH) pools and superoxide dismutase (SOD) activation, detoxification via phytochelatins and cell damage were monitored in metal-exposed exponential-phase cells and controls, grown in estuarine medium. Total SH and reduced+oxidized glutathione (GSH+GSSG) in T. pseudonana were much higher than in D. brightwellii. In T. pseudonana, total SH and GSH decreased at 322 nM Zn, and GSH increased at 80 nM Cu but decreased at 119 nM Cu. GSH:GSSG ratios were low, while phytochelatins were not detectable in metal-exposed D. brightwellii. Cd-exposed T. pseudonana made more phytochelatins than Cu-exposed cells, and in different proportions. At 322 nM Zn, SOD activity decreased in T. pseudonana. Zn caused a major, and Cu a minor increase of SOD activity in D. brightwellii; inhibition of photosynthesis was observed in Cu-exposed D. brightwellii, probably due to oxidative damage. The C:N ratios were higher and protein contents lower in Cu-exposed cells of both species, which might indicate excretion due to a loss of cell membrane integrity. From these results, it is hypothesized that T. pseudonana has evolved an effective detoxification mechanism as a result of a more severe exposure to toxic metals in rivers and estuaries. In contrast, D. brightwellii, a marine-estuarine species, cannot adjust well to metal exposure. Its poor defense against metal toxicity was marked by low SH-contents.
Rijstenbil
J W
JW
Netherlands Institute of Ecology, Centre for Estuarine and Coastal Ecology, Yerseke, The Netherlands.
Sandee
A
A
Van Drie
J
J
Wijnholds
J A
JA
D000975:Antioxidants; D017377:Diatoms / Q000187:drug effects* / Q000378:metabolism; D008658:Inactivation, Metabolic; D008670:Metals / Q000378:metabolism / Q000633:toxicity*; D018384:Oxidative Stress; D010788:Photosynthesis / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000502:physiology; D013482:Superoxide Dismutase / Q000502:physiology
D000975:Antioxidants; D008670:Metals; D013438:Sulfhydryl Compounds; D013482:Superoxide Dismutase