[Study on monoclonal antibodies against porcine serum E receptor].
Three hybridoma cell lines producing monoclonal antibodies (McAbs) against porcine serum E receptor (PSER) were established by fusing mouse myeloma SP2/o cells with spleen cells of BALB/c mouse immunized with PSER. The ascites McAbs were purified by ammonium sulfate precipitation and DEAE-cellulose chromatography. The McAbs were found to belong to the IgG1 and IgG2b subclasses, respectively. These McAbs inhibited reverse E-rosette formation and enhanced or depressed lymphocyte proliferation induced by PHA or ConA. The specificity of these McAbs was proved by immuno-dot blot and Western blot assay. The potential application of these McAbs in the study of structure and biological function of the E-receptor is discussed.
Yang
G
G
Institute of Basic Medical Sciences, Beijing.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D018801:CD2 Antigens; D002455:Cell Division; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D008214:Lymphocytes; D051379:Mice; D011971:Receptors, Immunologic / Q000276:immunology*; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D007074:Immunoglobulin G; D011971:Receptors, Immunologic