title: Hormonal modulation of atrial natriuretic factor receptors and effects on adrenal glomerulosa cells of female rats.
abstract: This study was done to determine if a decrease in the aldosterone-suppressant effect of atrial natriuretic factor (ANF) by progesterone and an increase by estrogen was caused by modulation of adrenal zona glomerulosa ANF receptors. Freshly dispersed glomerulosa cells from virgin, 13-15 day pregnant, ovariectomized (OVX) estradiol-17 beta-treated and OVX progesterone-treated rats were used. Competitive displacement of specifically bound [125I]ANF1-28 with unlabelled ANF1-28 yielded concentrations of guanylate cyclase-linked ANF-R1 plus ANF-R2 (clearance) receptors and the displacement with unlabelled ANF4-23 yielded ANF-R2 receptors; the difference between the two was treated as the concentration of ANF-R1 receptors. Pregnancy and progesterone decreased and estrogen increased the number of glomerulosa ANF-R1 receptors. ANF produced a significantly greater suppression of potassium-induced aldosterone secretion in cells from OVX estradiol-treated rats than in cells from OVX progesterone-treated animals. These data suggest that the inhibition of the aldosterone-suppressant activity of ANF by progesterone is the result of a downregulation of ANF-R1 receptors.
authors.affiliation: Royal Victoria Hospital, Department of Physiology, Montreal, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Mulay
authors.forename: S
authors.initials: S
authors.lastname: Omer
authors.forename: P
authors.initials: P
authors.lastname: Vaillancourt
authors.forename: S
authors.initials: S
authors.lastname: D'sylva
authors.forename: A
authors.initials: A
authors.lastname: Singh
authors.forename: D R
authors.initials: DR
authors.lastname: Varma
mesh_terms: D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D004347:Drug Interactions; D004357:Drug Synergism; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006162:Guanylate Cyclase / Q000187:drug effects / Q000502:physiology; D007457:Iodine Radioisotopes; D010052:Ovariectomy; D011188:Potassium / Q000494:pharmacology; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017461:Receptors, Atrial Natriuretic Factor / Q000187:drug effects* / Q000378:metabolism; D013268:Stimulation, Chemical; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology; D015384:Zona Glomerulosa / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D004967:Estrogens; D007457:Iodine Radioisotopes; D000450:Aldosterone; D011374:Progesterone; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; D011188:Potassium