title: Regulatory peptides in gastric endocrine cells of the rainbow trout Oncorhynchus mykiss: general distribution and colocalizations.
abstract: The endocrine cells of the rainbow trout (Oncorhynchus mykiss) stomach have been investigated using the immunocytochemical techniques of peroxidase-anti-peroxidase and avidin-biotin-peroxidase complexes on paraffin sections. 33 antisera were tested and eight immunoreactivities were detected: somatostatin-, glucagon- bombesin-, substance P-, serotonin-, met-enkephalin-, CCK/gastrin-, and chromogranin-like containing cells. All of them were present throughout the gastric mucosa except CCK/gastrin-like containing cells that were restricted to the pyloric epithelium. Somatostatin 25 and chromogranin immunoreactive cells are described for the first time in fish stomach. Serotonin immunoreactive cells were also positive for the Grimelius technique and some of them were immunoreactive to anti substance P or anti CCK/gastrin. Immunoreactivities for gastrin 17, gastrin 34 and CCK appeared in the same cells and the absorption controls showed that a molecule containing the carboxi-terminal pentapeptide of this family was present in trout stomach.
authors.affiliation: Department of Cytology and Histology, University of Navarra, Pamplona, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Barrenechea
authors.forename: J
authors.initials: J
authors.lastname: López
authors.forename: A
authors.initials: A
authors.lastname: Martínez
mesh_terms: D001078:APUD Cells / Q000378:metabolism*; D000818:Animals; D002766:Cholecystokinin / Q000032:analysis; D002864:Chromogranins / Q000032:analysis; D004744:Enkephalin, Methionine / Q000032:analysis; D005753:Gastric Mucosa / Q000737:chemistry* / Q000166:cytology; D005755:Gastrins / Q000032:analysis; D007124:Immunoenzyme Techniques; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010455:Peptides / Q000032:analysis*; D012701:Serotonin / Q000032:analysis
chemical_list: D002864:Chromogranins; D005755:Gastrins; D010455:Peptides; D012701:Serotonin; D004744:Enkephalin, Methionine; D002766:Cholecystokinin