Switching capacity of Fc epsilon RII-positive and -negative murine B cells.
In previous studies, our laboratory demonstrated the utility of the low affinity IgE Fc receptor (Fc epsilon RII) in delineating a number of murine B cell subsets. In the spleen, the Fc epsilon RII is expressed on mature conventional B cells but is absent on marginal zone B cells. In the peritoneal cavity, the receptor is present on all conventional B cells, but is not expressed on fresh peritoneal Ly1/sister B cells. The studies in this report compared the ability of these B cell populations to isotype switch. Using a lipopolysaccharide (LPS)- and interleukin (IL)-4-driven system, sort-purified Fc epsilon RII-positive and -negative B cells from peritoneum and spleen were tested for switching to IgG1, IgE, and IgA. The results demonstrated that regardless of their source, Fc epsilon RII+ B cells produced significant levels of IgG1 and IgE. Similar results were obtained with Fc epsilon RII- (marginal zone) B cells obtained from spleen. In contrast, Fc epsilon RII- (Ly1/sister) peritoneal B cells were found to produce IgG1 and IgA, but were incapable of secreting significant levels of IgE. Further studies tested for LPS and IL-4-induced expression of Fc epsilon RII and Thy1 on the various B cell populations. These experiments demonstrated the induction of the Fc epsilon RII on all B cells, regardless of their initial resting levels. Additionally, Thy1 was found to be induced only on those B cell subsets capable of producing IgE. Taken together, the results demonstrate a correlation between IgE secretion and Thy1 expression, and no apparent correlation between the presence of the Fc epsilon RII and isotype commitment.
Foy
T M
TM
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242.
Waldschmidt
T J
TJ
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D017578:Immunoglobulin Class Switching; D007072:Immunoglobulin D / Q000032:analysis; D015847:Interleukin-4 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D017455:Receptors, IgE / Q000032:analysis* / Q000096:biosynthesis; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D007072:Immunoglobulin D; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D017455:Receptors, IgE; D018800:Thy-1 Antigens; D015847:Interleukin-4