Effects of IFN-beta on growth of human prostatic JCA-1 cells.
Addition of IFN-beta resulted in a dose-dependent reduction of growth, a drop in [3H]thymidine incorporation into DNA, and a concurrent 69% and 15% increase in the S and G2/M phases, of the human prostatic JCA-1 cells. No correlation existed between the antimitogenicity of IFN and increases in the double-stranded RNA-dependent protein kinase activity. Although IFN elicited a large increase in 2-5A synthetase activity, activation of the 2-5A-dependent RNase L could not be demonstrated in JCA-1 cells rendered permeable to 2-5A, implying that the 2-5A pathway is not involved in the anti-proliferative effects of IFN. Analysis of endogenous proteins phosphorylated in vitro show that some IFN-inducible phosphoproteins were dependent upon the presence of double-stranded DNA.
Nakajima
Y
Y
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
Konno
S
S
Perruccio
L
L
Chen
Y
Y
Wu
J M
JM
An
S
S
Chiao
J
J
Choudhury
M
M
Mallouh
C
C
Muraki
J
J
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002846:Chromatography, Affinity; D004247:DNA / Q000096:biosynthesis; D004790:Enzyme Induction; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008938:Mitosis / Q000187:drug effects; D011067:Poly dA-dT / Q000494:pharmacology; D011467:Prostate; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D016196:S Phase / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D019892:eIF-2 Kinase
D011067:Poly dA-dT; D016899:Interferon-beta; D004247:DNA; D017346:Protein Serine-Threonine Kinases; D019892:eIF-2 Kinase; D015088:2',5'-Oligoadenylate Synthetase; D013936:Thymidine