Determination of 6-monoacetylmorphine and morphine in plasma, whole blood and urine using high-performance liquid chromatography with electrochemical detection.
6-Monoacetylmorphine and morphine were determined simultaneously in plasma, whole blood and urine, after solid-phase extraction, by high-performance liquid chromatography using amperometric detection at 600 mV oxidation potential. The recoveries ranged from 92 to 99%. The reproducibility study indicated that the coefficients of variation were less than 11% for morphine and 12.4% for 6-monoacetylmorphine. The determination limits were 1 ng/ml for morphine and 4 ng/ml for 6-monoacetylmorphine. The method had a good selectivity towards opiate and nonopiate analgesics and other drugs. The stability of the analytes in methanol (standard solutions), in samples (plasma, whole blood and urine) at -20 degrees C and at 20 degrees C, and in samples after enzymatic hydrolysis at 37 degrees C, was also studied. For sample containing 6-monoacetylmorphine, inadequate storage or hydrolysis could lead to overestimation of morphine or its conjugates. The technique described can be applied for the study of the pharmacokinetics of heroin; it is also available for forensic toxicology to distinguish heroin use from medical prescription of morphine and other opiate drugs.
Rop
P P
PP
Laboratoire Interrégional de Police Scientifique, Marseille, France.
Grimaldi
F
F
Burle
J
J
De Saint Leger
M N
MN
Viala
A
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D000432:Methanol; D009020:Morphine / Q000097:blood* / Q000652:urine*; D009022:Morphine Derivatives / Q000097:blood* / Q000652:urine*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D009022:Morphine Derivatives; D009020:Morphine; D005966:Glucuronidase; C026979:6-O-monoacetylmorphine; D000432:Methanol