title: Relationship between blood group-A antigen expression and malignant potential in hamster pancreatic cancers.
abstract: The loss of expression of the ABH blood group antigens is suggested to be associated with more aggressive behavior of cancers. We have compared the growth behaviors of two hamster pancreatic cancer cell lines with different blood group-A expressions. PC-1.0 cells, which expressed blood group-A antigen poorly, showed a faster growth in vitro and in vivo when implanted into the pancreas of homologous animals, whereas PC-1.2 cells, all of which express the antigen, had a slower growth rate both in vitro and in vivo. PC-1.0 also tended to metastasize, whereas PC-1.2 cells grew primarily locally. The allografts of both PC-1.2 cells (PC-1.2AG) and PC-1.0 cells (PC-1.0AG) and the metastases of PC-1.0 cells expressed blood group A antigen in a similar rate. There was no significant difference in the number of A-antigen positive cells (A+) between the PC-1.2AG and PC-1.0AG, although the expression of A antigen in PC-1.0AG showed a greater heterogeneity. The combined immunohistochemistry and autoradiography did not show any significant differences in the labeling index of A+ or A- cells between the two allografts. Thus, the results indicate that blood group A antigen expression is unrelated to malignancy in this model. The faster growth rate of PC-1.0 cells may be due to their shorter cell cycle.
authors.affiliation: Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-6805.
authors.forename: M
authors.initials: M
authors.lastname: Hirota
authors.forename: H
authors.initials: H
authors.lastname: Egami
authors.forename: M
authors.initials: M
authors.lastname: Mogaki
authors.forename: K
authors.initials: K
authors.lastname: Kazakoff
authors.forename: W G
authors.initials: WG
authors.lastname: Chaney
authors.forename: P M
authors.initials: PM
authors.lastname: Pour
mesh_terms: D000017:ABO Blood-Group System / Q000096:biosynthesis*; D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology; D000818:Animals; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D005700:Galactosyltransferases / Q000096:biosynthesis; D007124:Immunoenzyme Techniques; D007519:Isoantigens / Q000096:biosynthesis*; D008113:Liver Neoplasms / Q000556:secondary; D008647:Mesocricetus; D009362:Neoplasm Metastasis / Q000276:immunology; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000276:immunology* / Q000473:pathology
chemical_list: D000017:ABO Blood-Group System; D007519:Isoantigens; D005700:Galactosyltransferases; C047006:N-acetyllactosaminide alpha-1,3-galactosyltransferase