IFN-alpha inhibits antigen-induced eosinophil and CD4+ T cell recruitment into tissue.
In a murine model of airway late phase reaction, we have previously shown that Ag-induced eosinophil recruitment into the tissue of sensitized mice is mediated by CD4+ T cells and IL-5. To determine whether IFN-alpha regulates Ag-induced eosinophil recruitment into tissue, we studied the effects of rIFN-alpha, endogenous IFN-alpha production induced by poly I.C, and anti-IFN-alpha mAb on the eosinophil infiltration of the trachea induced by Ag inhalation in mice. The i.p. administration of rIFN-alpha prevented Ag-induced eosinophil infiltration in the trachea of sensitized mice. In addition, the inhibitory effect of rIFN-alpha on Ag-induced eosinophil infiltration was not recovered by pretreatment with anti-IFN-gamma mAb. The administration of rIFN-alpha also inhibited Ag-induced CD4+ T cell infiltration in the trachea. However, the administration of rIFN-alpha did not significantly affect blood eosinophil counts nor the number of blood and splenic CD4+ T cells. On the other hand, pretreatment with anti-murine IFN-alpha mAb enhanced Ag-induced eosinophil and CD4+ T cell infiltration in the trachea. Finally, endogenous IFN-alpha/beta production induced by poly I.C also inhibited Ag-induced eosinophil infiltration in the trachea and this inhibition of the eosinophil infiltration was abrogated by pretreatment with anti-IFN-alpha/beta mAb. These results indicate that IFN-alpha suppresses Ag-induced eosinophil and CD4+ T cell recruitment into tissue.
Nakajima
H
H
Department of Internal Medicine, Chiba University School of Medicine, Japan.
Nakao
A
A
Watanabe
Y
Y
Yoshida
S
S
Iwamoto
I
I
D000336:Aerosols; D000818:Animals; D000941:Antigens; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement; D004804:Eosinophils / Q000276:immunology*; D007249:Inflammation / Q000276:immunology; D016898:Interferon-alpha / Q000494:pharmacology*; D016899:Interferon-beta / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D011070:Poly I-C / Q000494:pharmacology; D014132:Trachea / Q000276:immunology
D000336:Aerosols; D000941:Antigens; D016898:Interferon-alpha; D016899:Interferon-beta; D010047:Ovalbumin; D011070:Poly I-C