Raphe pallidus stimulation increases gastric contractility via TRH projections to the dorsal vagal complex in rats.
The role of thyrotropin releasing hormone (TRH) in the dorsal vagal complex (DVC) in mediating the enhanced gastric contractility induced by glutamate (100 pmol) microinjected into the raphe pallidus (Rpa) was investigated in urethane-anesthetized rats acutely implanted with miniature strain gauge force transducers on the corpus of the stomach. Glutamate-induced stimulation of gastric contractility was dose-dependently inhibited by bilateral microinjection into the DVC of TRH antibody (0.17, 0.85 or 1.7 micrograms/100 nl/site) but not by vehicle. TRH antibody microinjected into the dorsal medullary reticular field had no effect. These data indicate that activation of Rpa neurons by glutamate increases gastric motor function through TRH release in the DVC.
Garrick
T
T
Department of Psychiatry, UCLA School of Medicine.
Prince
M
M
Yang
H
H
Ohning
G
G
Taché
Y
Y
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005769:Gastrointestinal Motility / Q000502:physiology*; D005917:Globus Pallidus / Q000502:physiology*; D005971:Glutamates / Q000008:administration & dosage / Q000494:pharmacology; D018698:Glutamic Acid; D008297:Male; D008845:Microinjections; D009119:Muscle Contraction / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D011903:Raphe Nuclei / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013973:Thyrotropin-Releasing Hormone / Q000276:immunology / Q000502:physiology*; D014630:Vagus Nerve / Q000502:physiology*
D005971:Glutamates; D018698:Glutamic Acid; D013973:Thyrotropin-Releasing Hormone; D002217:Carbachol