Developmental expression of a novel murine homeobox gene (Chx10): evidence for roles in determination of the neuroretina and inner nuclear layer.
Few potential regulatory proteins of vertebrate retinal development have been identified. We describe a 39 kDa murine polypeptide (Chx10) with a homeodomain 82% identical to that of the nematode protein ceh-10. In the developing mouse, the Chx10 transcript is expressed throughout the anterior optic vesicle and all neuroblasts of the optic cup. In the mature retina, the Chx10 protein is restricted to the inner nuclear layer, in which its expression decreases from the outer to the inner margin. Chx10 transcripts are also detected in regions of the developing thalamus, hindbrain, and ventral spinal cord. The data suggest that Chx10 plays critical roles in the formation of the neuroretina and in the development and maintenance of the inner nuclear layer.
Liu
I S
IS
Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
Chen
J D
JD
Ploder
L
L
Vidgen
D
D
van der Kooy
D
D
Kalnins
V I
VI
McInnes
R R
RR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology; D001931:Brain Mapping; D003001:Cloning, Molecular; D015870:Gene Expression; D005796:Genes; D005801:Genes, Homeobox; D018257:Helix-Loop-Helix Motifs; D018398:Homeodomain Proteins; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009046:Motor Neurons / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D012160:Retina / Q000196:embryology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology*
D018398:Homeodomain Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C088975:Vsx2 protein, mouse