title: Cloning of an apamin binding protein of vascular smooth muscle.
abstract: The receptor for the bee venom derived neurotoxin, apamin, is widely believed to be an integral component of the small conductance calcium-activated potassium channel in many excitable cells. By affinity chromatography on immobilized apamin, a 78 kD apamin binding protein of the bovine brain synaptosomes was isolated. Antibodies were elicited against this protein and used to clone a cDNA from a porcine vascular smooth muscle expression library. This gene (Kcal 1.8) codes for a 438 amino protein with four potential transmembrane domains, one putative calcium binding site, a protein kinase C phosphorylation site, and a leucine zipper motif. Kcal 1.8 encoded protein has no significant sequence homologies with any known ion channels or receptors. Kcal 1.8 is likely to encode a protein associated with the small conductance calcium-activated potassium channel in vascular smooth muscle.
authors.affiliation: Department of Molecular Pharmacology, American Cyanamid Company, Lederle Laboratories, Pearl River, New York 10965.
authors.forename: P T
authors.initials: PT
authors.lastname: Sokol
authors.forename: W
authors.initials: W
authors.lastname: Hu
authors.forename: L
authors.initials: L
authors.lastname: Yi
authors.forename: J
authors.initials: J
authors.lastname: Toral
authors.forename: M
authors.initials: M
authors.lastname: Chandra
authors.forename: M R
authors.initials: MR
authors.lastname: Ziai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001030:Apamin / Q000378:metabolism*; D001665:Binding Sites; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002846:Chromatography, Affinity; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D006801:Humans; D016350:Leucine Zippers; D008545:Melanoma; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009447:Neuroblastoma; D011061:Poly A / Q000032:analysis / Q000096:biosynthesis; D015221:Potassium Channels / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D013552:Swine; D013574:Synaptosomes / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D015221:Potassium Channels; D012333:RNA, Messenger; D011994:Recombinant Proteins; C033799:apamin receptor; D001030:Apamin; D011061:Poly A; D002118:Calcium