The human myocardial two-dimensional gel protein database: update 1994.
An updated human heart protein two-dimensional electrophoresis (2-DE) database is presented. The database, which contains some 1388 protein spots characterised in terms of M(r) and pI, has been analysed further by Western immunoblotting and protein sequencing. From a total of 103 protein spots analysed, 49 have been identified by immunoblotting and 32 have been identified by protein sequencing. A further six proteins have tentatively been assigned by comparison with the human heart 2-DE protein database of Jungblut et al. (Electrophoresis) 1994, 15, 685-607). This database is being used in studies of alterations in protein expression in the diseased and transplanted human heart.
Corbett
J M
JM
Department of Cardiothoracic Surgery, National Heart and Lung Institute, Heart Science Centre, Middlesex, UK.
Wheeler
C H
CH
Baker
C S
CS
Yacoub
M H
MH
Dunn
M J
MJ
D000595:Amino Acid Sequence; D015153:Blotting, Western; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D006331:Heart Diseases / Q000378:metabolism; D016027:Heart Transplantation; D006352:Heart Ventricles; D006801:Humans; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry* / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification
D011506:Proteins