Sensitive method for the assay of sertindole in plasma by high-performance liquid chromatography and fluorimetric detection.
A simple and highly sensitive normal-phase HPLC method is described for determining sertindole concentrations in human plasma using fluorimetric detection. A short C8 column was used to extract sertindole and the internal standard from plasma; the column was rinsed with acetonitrile, and the analytes were recovered by elution with methanol. This uncommon selectivity between the two solvents allowed clean extraction and near- quantitative recovery of the analytes (> 89%). Separation was done on a 5-microns silica-gel column and detection was performed by fluorimetry, with emission at 340 nm and excitation at 260 nm. The detection and lower quantifiable limits were 0.01 and 0.025 ng/ml, respectively, with no interference from plasma or potential metabolites.
Tzeng
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Drug Metabolism Department, Abbott Laboratories, Abbott Park, IL 60064-3500.
Stamm
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Chu
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D014150:Antipsychotic Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D007093:Imidazoles / Q000097:blood*; D007211:Indoles / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D014150:Antipsychotic Agents; D007093:Imidazoles; D007211:Indoles; C066304:sertindole