Stimulation of in vitro ovulation and contraction of brook trout (Salvelinus fontinalis) follicles by adrenaline through alpha-adrenoreceptors.
The effects of adrenaline and adrenoreceptor antagonists on ovulation and follicle wall contraction were investigated in brook trout (Salvelinus fontinalis) follicles using in vitro incubation systems. Adrenaline significantly stimulated a dose-dependent increase in ovulation and follicle contraction at concentrations between 1.0 and 100 mumol l-1. The ovulatory and contractile effects of 10 mumol adrenaline l-1 could be blocked by the alpha 1-adrenoreceptor antagonists WB-4101 and benoxathian, and by the alpha 2-antagonist yohimbine. WB-4101 was the most potent blocker, significantly inhibiting ovulation and contraction at 1.0 mumol l-1. In contrast, the beta-antagonist propranolol (100-0.001 mumol l-1) was totally ineffective in blocking adrenaline-induced ovulation and follicle contraction. The results indicate that there is a strong correlation between the effects of adrenaline on ovulation and contraction. In addition, the antagonist studies indicate that adrenaline stimulates ovulation and follicle contraction of brook trout follicles through alpha-adrenoreceptors.
Goetz
F W
FW
University of Notre Dame, Department of Biological Sciences, ID 46556.
Bradley
J A
JA
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D004146:Dioxanes / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D010060:Ovulation / Q000187:drug effects* / Q000502:physiology; D010075:Oxathiins / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D013268:Stimulation, Chemical; D014337:Trout / Q000502:physiology*; D015016:Yohimbine / Q000494:pharmacology
D000317:Adrenergic alpha-Antagonists; D004146:Dioxanes; D010075:Oxathiins; D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine; C050877:benoxathian; D011433:Propranolol; C010654:(2-(2',6'-dimethoxy)phenoxyethylamino)methylbenzo-1,4-dioxane; D004837:Epinephrine