Sequencing and analysis of the nucleocapsid (N) and polymerase (L) genes and the terminal extragenic domains of the vaccine strain of rinderpest virus.
The nucleocapsid (N) and polymerase (L) genes of the vaccine strain of rinderpest, and the 5' and 3' terminal domains of the genome have been sequenced. Together with previously published data, this completes the sequence of the entire genome of rinderpest virus. The viral genome is 15,881 bases in length, similar to that of measles virus and slightly longer than that of canine distemper virus. The L gene is identical in length to that of measles virus, encoding a 2183 amino acid protein with a calculated M(r) of 248,100. The L protein sequence of morbilliviruses is highly conserved, more than 75% of residues being identical or conserved in all three sequences currently available. The N protein was, as for the other sequenced genes where comparison is possible, essentially identical to that of the virulent parent. In addition, we have determined the terminal sequences of two virulent strains of rinderpest and compared the sequences of virulent and non-virulent strains.
Baron
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AFRC Institute for Animal Health, Pirbright Laboratory, Surrey, UK.
Barrett
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D012302:Rinderpest virus / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014758:Viral Core Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics*
D012367:RNA, Viral; D014758:Viral Core Proteins; D014765:Viral Vaccines; D012321:DNA-Directed RNA Polymerases