title: Lipid determination from monophasic solvent mixtures: influence of uneven distribution of lipids after filtration and centrifugation.
abstract: Reasons for the variation in erythrocyte lipid extraction were investigated. We found that there was an uneven distribution of lipids after filtration (up to 10% accumulated in the filtrates) or centrifugation (up to 10% in the top fraction) of several apparently monophasic extraction systems. The uneven distribution was more pronounced in the systems containing relatively more water, or, generally speaking, with more nonpolar solvents. Ill-defined procedures using either filtration or centrifugation, therefore, could be the cause for variations in lipid extraction. A way to prevent the uneven distribution has been developed and is discussed.
authors.affiliation: Department of Cell Biology I, University Hospital of Lund, Sweden.
authors.forename: W Q
authors.initials: WQ
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Gustafson
mesh_terms: D000433:1-Propanol; D002498:Centrifugation; D002725:Chloroform; D004912:Erythrocytes / Q000737:chemistry*; D000431:Ethanol; D005374:Filtration; D006586:Hexanes; D006801:Humans; D008055:Lipids / Q000097:blood*; D000432:Methanol; D012997:Solvents
chemical_list: D006586:Hexanes; D008055:Lipids; D012997:Solvents; D000431:Ethanol; D002725:Chloroform; D000433:1-Propanol; D000432:Methanol