title: Glucose can fuel glutamate uptake in ischemic brain.
abstract: Astrocytes in culture can maintain glutamate uptake during hypoxia if glucose is available. To determine whether this capacity is shared by brain in situ, extracellular glutamate levels were measured in ischemic brain under conditions of continued glucose delivery. Microdialysis probes were placed bilaterally in caudate nuclei of rats and perfused with artificial cerebrospinal fluid (CSF) containing either 30 or 0 mM glucose. Global cerebral ischemia was induced by cardiac arrest. Dialysate collected from probes not perfused with glucose showed a 50-fold increase in glutamate levels over the 60 min following cardiac arrest. Addition of glucose to the perfusate reduced the glutamate rise to < 20% of the levels attained in the glucose-free probes. The glucose effect was negated by the addition of 0.5 mM of the glutamate uptake blocker threo-beta-hydroxyaspartate to the artificial CSF. These results show that oxygen is not required to maintain efficient uptake of extracellular glutamate in brain and suggest that elevations in extracellular glutamate levels during ischemia result from metabolic perturbations other than hypoxia.
authors.affiliation: Department of Neurology, Veterans Affairs Medical Center, San Francisco, California 94121.
authors.forename: R A
authors.initials: RA
authors.lastname: Swanson
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: S H
authors.initials: SH
authors.lastname: Graham
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D005947:Glucose / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D008297:Male; D017551:Microdialysis; D009994:Osmolar Concentration; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000596:Amino Acids; D005971:Glutamates; C011274:3-hydroxyaspartic acid; D001224:Aspartic Acid; D018698:Glutamic Acid; D005947:Glucose