P-glycoprotein expression in Crassostrea gigas and Mytilus edulis in polluted seawater.
1. DNA-DNA hybridization showed that mussels (Mytilus edulis) and oysters (Crassostrea gigas) possess a highly conserved region constituting one or more genes of the ATP-dependent transmembrane transporter family to which belong the mammalian tumor cell genes responsible for multi-drug resistance. 2. Monoclonal antibody C219 visualized two proteins (220 and 240 kDa) which varied quantitatively in mussels depending on the level of organic pollution at the sampling sites. 3. The authors consider whether certain pollutants in the marine environment are capable of inducing pollutant-resistant gene expression.
Minier
C
C
IFREMER, Nantes, France.
Akcha
F
F
Galgani
F
F
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D005796:Genes; D015151:Immunoblotting; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D010125:Ostreidae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012623:Seawater; D014874:Water Pollutants, Chemical / Q000032:analysis; D014877:Water Pollution, Chemical
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D009838:Oligodeoxyribonucleotides; D014874:Water Pollutants, Chemical