title: Alterations in oncogene expression and radiosensitivity in the most frequently used SV40-transformed human skin fibroblasts.
abstract: In comparison with primary cell cultures, SV40-transformed human skin fibroblasts, either from healthy donors or from patients suffering from ataxia-telangiectasia (AT) or xeroderma pigmentosum, are more resistant to the cytotoxic action of low LET 60cobalt gamma-rays as well as to high LET alpha-particles. Resistance factors calculated from D10's lie between 1.4 and 2.0. Northern blot analysis reveals spontaneous overexpression of the oncogenes c-myc, Ki-ras and c-raf and of the tumour suppressor gene p53 as a consequence of SV40 transformation. For c-myc, the increased expression is due to gene amplification and gene rearrangement. An even further increase in the expression of c-myc has been found for AT cells (AT5BI-VA) after moderate doses of 60cobalt gamma-irradiation. A possible correlation between SV40-induced changes in gene expression and cellular radioresistance is discussed.
authors.affiliation: Kernforschungszentrum Karlsruhe, Institut für Genetik, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Lücke-Huhle
mesh_terms: D000512:Alpha Particles; D000576:Americium; D002472:Cell Transformation, Viral / Q000235:genetics*; D003037:Cobalt Radioisotopes; D004735:Energy Transfer; D005720:Gamma Rays; D005784:Gene Amplification; D015967:Gene Expression Regulation, Viral / Q000528:radiation effects*; D016259:Genes, myc; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D009857:Oncogenes; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019908:Proto-Oncogene Proteins c-raf; D013539:Simian virus 40 / Q000235:genetics*; D012867:Skin
chemical_list: D003037:Cobalt Radioisotopes; D011518:Proto-Oncogene Proteins; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf; D000576:Americium