title: Therapeutic effect of intradermal injections with difucosyl lactosamine (dimeric Lex) on patients with rheumatoid arthritis.
abstract: As reported by us, a new myeloid cell population with an oncofetal membrane marker, dimeric Lex (di-Lex; III3FucV3 FucnLc6), was found in the epiphyseal bone marrow adjacent to the involved joints of patients with severe rheumatoid arthritis (RA). Patients with RA received intradermal (id) injections of di-Lex incorporated in liposome or of high molecular weight glycoprotein, or tumor associated carbohydrate antigen (TCA), containing the same carbohydrate epitope as di-Lex. The epiphyseal myeloid cells were reduced or sometimes eliminated during id injection. In random trials of id injection, observation under clinical and laboratory conditions showed improvement in 63% (17/27) of the patients treated for 6 months with appropriate doses of di-Lex (III3FucnLc4), and in 72% (31/43) of those treated with an identical protocol for TCA. However, id injection with monomeric Lex had no effect.
authors.affiliation: Department of Orthopedic Surgery, Osaka University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ochi
authors.forename: S
authors.initials: S
authors.lastname: Hakomori
authors.forename: M
authors.initials: M
authors.lastname: Fujimoto
authors.forename: M
authors.initials: M
authors.lastname: Okamura
authors.forename: H
authors.initials: H
authors.lastname: Owaki
authors.forename: S
authors.initials: S
authors.lastname: Wakitani
authors.forename: Y
authors.initials: Y
authors.lastname: Shimaoka
authors.forename: K
authors.initials: K
authors.lastname: Hayashida
authors.forename: T
authors.initials: T
authors.lastname: Tomita
authors.forename: S
authors.initials: S
authors.lastname: Kawamura
mesh_terms: D000328:Adult; D000368:Aged; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000008:administration & dosage; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000473:pathology; D001853:Bone Marrow / Q000473:pathology; D002240:Carbohydrate Sequence; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D005260:Female; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000008:administration & dosage; D006801:Humans; D007279:Injections, Subcutaneous; D016256:Lewis X Antigen / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use*; D008081:Liposomes; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D013997:Time Factors
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D004337:Drug Carriers; D006023:Glycoproteins; D016256:Lewis X Antigen; D008081:Liposomes; C069719:sialosyl dimeric Le(x) antigen