Structure and function of the mouse surfactant protein B gene.
The mouse surfactant protein B (SP-B) gene was isolated from a DBA/2J EMBL3 genomic library, and the nucleotide sequence and transcriptional start site was determined. The SP-B gene was comprised of 11 exons and 10 introns and spanned 9,882 bases. Repetitive elements of the B1, B2, and R families and several other motifs were located within nontranslated regions of the gene. The 5' flanking region of the gene was utilized to express a bacterial chloramphenicol acetyl transferase reporter gene in mouse and human pulmonary adenocarcinoma cell lines. Transcriptionally active regions of the promoter were identified that determined lung epithelial cell-specific gene expression. Regions of the gene promoter shared between the mouse and human SP-B gene were used to locate putative transcription factor binding sites for thyroid transcription factor-1 and hepatocyte nuclear factor-3. The 5' flanking region of the murine SP-B contains cell-specific cis-active elements that regulate SP-B expression in the respiratory epithelium.
Bruno
M A
MA
Children's Hospital Research Foundation, Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio 45229.
Bohinski
R J
RJ
Carter
J E
JE
Foss
K A
KA
Whitsett
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D018076:DNA, Complementary / Q000737:chemistry; D015723:Gene Library; D017930:Genes, Reporter; D006801:Humans; D008099:Liver / Q000737:chemistry; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011510:Proteolipids / Q000737:chemistry / Q000235:genetics*; D011663:Pulmonary Surfactants / Q000737:chemistry*; D015183:Restriction Mapping; D017385:Sequence Homology; D014162:Transfection
D018076:DNA, Complementary; D011510:Proteolipids; D011663:Pulmonary Surfactants; D004247:DNA