title: Effect of vitamin C on antioxidants, lipid peroxidation, and GSH system in the normal guinea pig heart.
abstract: Male guinea pigs were fed during 5 weeks with diets differing only in vitamin C content: low (33 mg/kg diet), medium (660 mg/kg), and high (13,200 mg/kg). Heart vitamin C was strongly dependent on dietary vitamin C and heart vitamin E showed a trend to increase as a function of the vitamin C level in the diet. The low vitamin C diet decreased body weight gain, food intake, and heart malondialdehyde without changing lipid peroxidation, whereas the high vitamin C increased oxidized glutathione and glutathione peroxidase and decreased body growth. A tendency to show higher levels of all the first-line antioxidants reduced glutathione, uric acid, superoxide dismutase, catalase, and glutathione peroxidase at extreme (high or low) dietary levels of vitamin C was observed. The guinea pig heart showed capacity for enzymatic but not for non-enzymatic in vitro lipid peroxidation. It is concluded that dietary vitamin C supplementation is able to increase the global antioxidant capacity of the heart tissue.
authors.affiliation: Department of Animal Biology-II (Animal Physiology), Faculty of Biology, Complutense University, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Rojas
authors.forename: S
authors.initials: S
authors.lastname: Cadenas
authors.forename: R
authors.initials: R
authors.lastname: Pérez-Campo
authors.forename: M
authors.initials: M
authors.lastname: López-Torres
authors.forename: G
authors.initials: G
authors.lastname: Barja
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004032:Diet; D004435:Eating / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D014527:Uric Acid; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid