Transient expression of the recombinant chick brain P2y1 purinoceptor and localization of the corresponding mRNA.
Extracellular ATP acts at specific cell surface purinoceptors to elicit a wide range of physiological responses. We have recently isolated a cDNA for a G-protein-coupled P2 purinoceptor (P2y1) from chick brain. It has been defined as a P2Y-like purinoceptor by the rank order of potency of P2 purinoceptor ligands, determined electrophysiologically in the Xenopus oocyte expression system. Here, we examine the ligand selectivity of this recombinant receptor, expressed transiently in COS-7 cells. The regional distribution of the P2y1 purinoceptor transcript within the one-day-post-hatch chick brain was also determined. It is widely expressed in the cerebellum and telencephalon and in specific nuclei of the mesencephalon and diencephalon, suggesting a neuronal localization of the P21 purinoceptor.
Webb
T E
TE
Molecular Neurobiology Unit, Royal Free Hospital School of Medicine, London, United Kingdom.
Simon
J
J
Bateson
A N
AN
Barnard
E A
EA
D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D001923:Brain Chemistry; D002460:Cell Line; D002645:Chickens / Q000378:metabolism*; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D008024:Ligands; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011983:Receptors, Purinergic / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*
D018076:DNA, Complementary; D008024:Ligands; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011983:Receptors, Purinergic; D011993:Recombinant Fusion Proteins; D000255:Adenosine Triphosphate