Evaluation of chemiluminescent detection of bromodeoxyuridine-labeled DNA.
Colorimetric immunoenzymatic procedures using monoclonal antibodies to detect 5-bromo-2-deoxy-uridine-DNA in hybridization experiments are not sensitive enough. We have developed a chemiluminescent immunodot assay to improve the sensitivity of the immunoenzymatic procedure, and attained a suitable detection level for single-stranded DNA. This new chemiluminescent immunodot assay has also been used to detect our recently synthesized cytidine analogue by means of its corresponding monoclonal antibody.
López-Canovas
L
L
Department of Molecular Biology, Centro Nacional de Investigaciones Científicas, Havana, Cuba.
López-Brauet
A
A
Del Castillo-Bilbao
M D
MD
Rodríguez-Tanty
C
C
Higginson-Clarke
D
D
Pascual-Márquez
C
C
Macías-Cabrera
A
A
Riverón-Rojas
A M
AM
D001973:Bromodeoxyuridine; D003124:Colorimetry; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis*; D005069:Evaluation Studies as Topic; D015151:Immunoblotting; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization
D004277:DNA, Single-Stranded; D004247:DNA; D001973:Bromodeoxyuridine