The promoter of coconut foliar decay-associated circular single-stranded DNA directs phloem-specific reporter gene expression in transgenic tobacco.
A full-length double-stranded DNA copy of the single-stranded circular DNA associated with coconut foliar decay virus (CFDV) was constructed. Full-length CFDV DNA and smaller fragments were transcriptionally fused to the beta-glucuronidase reporter gene and examined for promoter activity in vivo. In stably transformed tobacco plants, the CFDV DNA promoter confered a tissue-specific expression pattern in that the reporter gene was specifically expressed in the phloem tissue of the vascular system in stem, leaves and flower. These results are in agreement with the previously reported association of CFDV DNA with the phloem of its coconut host plant.
Rohde
W
W
MPI für Züchtungsforschung, Köln, Germany.
Becker
D
D
Randles
J W
JW
D001483:Base Sequence; D003059:Cocos / Q000821:virology*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D017791:Geminiviridae / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D018515:Plant Leaves / Q000235:genetics; D018547:Plant Stems / Q000235:genetics; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D005966:Glucuronidase