Lymphocyte adhesion to brain capillary endothelial cells in vitro.
The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1 beta (rhIL-1 beta), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries. Adhesion of lymphocytes to BBEC increased 4.1-fold after stimulation of the endothelial cells for 4 h with 5 or 10 ng/ml LPS. Lymphocyte adhesion increased after incubation of the BBEC for 4 h with IL-1 and was increased 3.7-fold using 100 ng/ml IL-1. BBEC pre-incubated with IL-6 for 4 h also showed an increase in adhesion of lymphocytes, and cells pretreated with 100 ng/ml IL-6 showed a 3-fold increase in lymphocyte adherence. Specific monoclonal antibodies directed against CD11a, CD18, and VLA-4 were able to block adherence of lymphocytes to stimulated BBEC. These results indicate that the in vitro activation of BBEC may serve as a model for the study of inflammation of the blood-brain barrier.
de Vries
H E
HE
Division of Pharmacology, Leiden/Amsterdam Center for Drug Research, University of Leiden, Sylvius Laboratories, The Netherlands.
Moor
A C
AC
Blom-Roosemalen
M C
MC
de Boer
A G
AG
Breimer
D D
DD
van Berkel
T J
TJ
Kuiper
J
J
D000818:Animals; D015703:Antigens, CD / Q000502:physiology; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply*; D018821:CD18 Antigens; D002196:Capillaries / Q000166:cytology; D002417:Cattle; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1; D008070:Lipopolysaccharides / Q000494:pharmacology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology*; D016029:Receptors, Very Late Antigen / Q000502:physiology
D015703:Antigens, CD; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D008070:Lipopolysaccharides; D016169:Lymphocyte Function-Associated Antigen-1; D016029:Receptors, Very Late Antigen; D018799:Intercellular Adhesion Molecule-1