title: Potentiation of N-methyl-D-aspartate receptor-dependent afterdischarges in rat dentate gyrus following in vitro ethanol withdrawal.
abstract: The contribution of NMDA receptors to neuronal hyperexcitability following in vitro ethanol withdrawal was examined using afterdischarges (ADs) in rat dentate gyrus as a model system. ADs were evoked by high frequency stimuli and blocked by N-methyl-D-aspartate (NMDA) receptor antagonists. Ethanol (75 mM) inhibited ADs, and following in vitro ethanol withdrawal, the duration of afterdischarges was significantly enhanced (37 +/- 2%, n = 21). Therefore, in vitro ethanol exposure with subsequent withdrawal is associated with an enhancement of NMDA receptor-dependent afterdischarges. These results are consistent with the involvement of NMDA receptors in ethanol withdrawal hyperexcitability.
authors.affiliation: Department of Pharmacology, University of Nebraska Medical Center, Omaha 68198-6260.
authors.forename: R A
authors.initials: RA
authors.lastname: Morrisett
mesh_terms: D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D004594:Electrophysiology; D000431:Ethanol / Q000009:adverse effects* / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000503:physiopathology*; D007736:Kynurenic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000502:physiology*; D013375:Substance Withdrawal Syndrome / Q000503:physiopathology*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D000431:Ethanol; D015763:2-Amino-5-phosphonovalerate; D007736:Kynurenic Acid; D008274:Magnesium; C057013:7-chlorokynurenic acid