title: MR lymphography with a lymphotropic T1-type MR contrast agent: Gd-DTPA-PGM.
abstract: A model system of a paramagnetic lymphotropic MR contrast agent (Gd-DTPA labeled polyglucose associated macrocomplex, PGM) for T1-weighted MR imaging of lymph nodes in rats and rabbits was evaluated. Pharmacokinetic (tissue accumulation) and MR imaging data (optimal dose and timing parameters) were obtained in normal rats (n = 88) after subcutaneous (SC) injection of paramagnetic, radiolabeled [111In]Gd-DTPA-PGM. A rabbit model of lymph node metastases (n = 8) was ultimately used to demonstrate the potential of MR imaging with Gd-DTPA-PGM for nodal tumor detection. Maximum concentrations of Gd-DTPA-PGM were found in popliteal and paraaortic lymph nodes within 24 h after SC administration, and highest lymph node SNR values were obtained by MR imaging at this time point. The optimum imaging dose was 6-12 mumol Gd/kg. Tumor-lymph node contrast increased from 0.0 +/- 1.2 precontrast to 19.2 +/- 6.5 (spoiled gradient echo sequence, TR 50/TE 7/flip angle 60 degrees) postcontrast and conspicuity of nodal metastases was improved. Gd-DTPA-PGM accumulates in lymph nodes after SC administration and significantly enhances lymph node signal intensity of normal animals but not metastatic lymph nodes.
authors.affiliation: Department of Radiology, Massachusetts General Hospital, Boston 02114.
authors.forename: L
authors.initials: L
authors.lastname: Harika
authors.forename: R
authors.initials: R
authors.lastname: Weissleder
authors.forename: K
authors.initials: K
authors.lastname: Poss
authors.forename: C
authors.initials: C
authors.lastname: Zimmer
authors.forename: M I
authors.initials: MI
authors.lastname: Papisov
authors.forename: T J
authors.initials: TJ
authors.lastname: Brady
mesh_terms: D000818:Animals; D003287:Contrast Media; D019786:Gadolinium DTPA; D007205:Indium Radioisotopes; D007279:Injections, Subcutaneous; D008198:Lymph Nodes / Q000033:anatomy & histology*; D008207:Lymphatic Metastasis / Q000175:diagnosis; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D009942:Organometallic Compounds; D004369:Pentetic Acid / Q000031:analogs & derivatives*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D003287:Contrast Media; D007205:Indium Radioisotopes; D009942:Organometallic Compounds; D004369:Pentetic Acid; D019786:Gadolinium DTPA