Two chronic myelogenous leukaemia cell lines which represent different stages of erythroid differentiation.
We established two cell lines, YN-1 and Y-1K, from the peripheral blood of two chronic myelogenous leukaemia patients in blastic crisis. Characterization of the YN-1 and Y-1K cells revealed that these cells expressed erythroid lineage markers. However, there was a marked difference in the level of gamma-globin mRNA and haemoglobin in YN-1 and Y-1K cells. YN-1 contained approximately 1-5% benzidine-positive staining cells, whereas no benzidine-positive cells were observed in Y-1K cells. Haemoglobin production in YN-1 cells was markedly increased with various chemical inducers of erythroid differentiation, but was not in Y-1K cells. In contrast, Y-1K cells expressed CD34 stem cell antigen and CD41 megakaryocyte-specific antigen. These observations suggested that, although both cell lines were committed to the erythroid lineage, each cell line represented a distinct differentiation stage in the erythroid differentiation programme. Y-1K seemed to correspond to an early stage of cells in erythroid lineage, whereas YN-1 represented a more advanced stage in human erythropoiesis.
Endo
K
K
Second Department of Internal Medicine, Tohoku University, Sendai, Japan.
Harigae
H
H
Nagai
T
T
Fujie
H
H
Meguro
K
K
Watanabe
N
N
Furuyama
K
K
Kameoka
J
J
Okuda
M
M
Hayashi
N
N
D000328:Adult; D002454:Cell Differentiation; D004305:Dose-Response Relationship, Drug; D004900:Erythroblasts / Q000648:ultrastructure; D015672:Erythroid Precursor Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015321:Gene Rearrangement; D005914:Globins / Q000032:analysis; D006427:Hemin / Q000378:metabolism; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D006454:Hemoglobins; D012333:RNA, Messenger; D006427:Hemin; D005914:Globins