[Cholecalciferol Reference Standard (Control 921) of National Institute of Health Sciences].
The raw material for cholecalciferol was tested for preparation of the "Cholecalciferol Reference Standard (Control 921)". Analytical data obtained were as follows: melting point, 89.9 degrees C; UV and infrared spectra, the same as those for JP Cholecalciferol Reference Standard (Control 901), respectively; specific absorbance at 265 nm E1%1 cm = 472.2; optical rotation, [alpha]20D = 107.1 degrees; thin-layer chromatography and high-performance liquid chromatography (HPLC), no impurities were detected; assay, 99.97% by HPLC. Based on the above results, the raw material was authorized as the Japanese Pharmacopoeia Reference Standard (Control 921).
Izumi
W
W
Kitajima
A
A
Yoshii
K
K
Komatsu
H
H
Ishimitsu
S
S
Okada
S
S
D002762:Cholecalciferol / Q000302:isolation & purification / Q000592:standards*; D002851:Chromatography, High Pressure Liquid; D006077:Government Agencies; D006296:Health Services; D007564:Japan; D010602:Pharmacopoeias as Topic; D013055:Spectrophotometry, Infrared
D002762:Cholecalciferol