Enzyme defects in glutamate-requiring strains of Schizosaccharomyces pombe.
Among the glutamate-requiring strains of Schizosaccharomyces pombe previously described [1], glu2 and glu3 strains were both shown to lack NAD-specific isocitrate dehydrogenase. glu4 strains were shown to lack glutamine:2-oxoglutarate aminotransferase (GOGAT), and to be defective in ammonia assimilation. The regulation of GOGAT activity in wild-type cells was investigated and was consistent with GOGAT and glutamine synthetase being involved in ammonium assimilation, particularly under conditions of nitrogen limitation.
Barel
I
I
Department of Genetics, University of Cambridge, UK.
MacDonald
D W
DW
D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005970:Glutamate Synthase / Q000235:genetics; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D007521:Isocitrate Dehydrogenase / Q000235:genetics; D009154:Mutation / Q000502:physiology; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D005971:Glutamates; D018698:Glutamic Acid; D007521:Isocitrate Dehydrogenase; D005970:Glutamate Synthase