title: Glutathione S-transferase and gamma-glutamyl transpeptidase activities in cultured rat hepatocytes treated with tocotrienol and tocopherol.
abstract: 1. The effect of tocotrienol and tocopherol on glutathione S-transferase (GST) and gamma-glutamyl transpeptidase (GGT) activities in cultured rat hepatocytes were investigated. 2. Tocotrienol and tocopherol significantly decreased GGT activities at 5 days in culture but tocotrienol also significantly decreased GGT activities at 1-2 days. 3. Tocotrienol and tocopherol treatment significantly decreased GST activities at 3 days compared to the control but tocotrienol also decreased GST activities at 1-3 days. 4. Tocotrienol showed a more pronounced effect at a dosage of greater than 50 microM tocotrienol at 1-3 days in culture compared to the control.
authors.affiliation: Jabatan Biokimia, Fakulti Perubatan, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala, Lumpur.
authors.forename: F B
authors.initials: FB
authors.lastname: Ong
authors.forename: W Z
authors.initials: WZ
authors.lastname: Wan Ngah
authors.forename: N A
authors.initials: NA
authors.lastname: Shamaan
authors.forename: A G
authors.initials: AG
authors.lastname: Md Top
authors.forename: A
authors.initials: A
authors.lastname: Marzuki
authors.forename: A K
authors.initials: AK
authors.lastname: Khalid
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
chemical_list: D002839:Chromans; D014810:Vitamin E; C013649:plastochromanol 8; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase