Superoxide dismutase and catalase activity in psoriatic patients treated topically with ointment containing 2-chloroethyl-3-chloropropyl sulfide.
The purpose of this study was to determine dismutase and catalase activities in erythrocytes of psoriatic patients with psoriasis vulgaris topically treated with an ointment (in accordance with recommendations of the Helsinki Declaration), in which 2-chloroethyl-3-chloropropyl sulfide (CLEPS) is an active compound. SOD activity in hemolysates was determined according to the method of Misra and Fridovich [12] and calculated as units per g of hemoglobin. CAT activity in hemolysates was determined by Beers and Sizer method [2] and expressed in U/g Hb. SOD activity in the control group was 1.61 +/- 0.48 U/g Hb x 10(3). However, the activity of CAT was 5.72 +/- 1.17 U/g Hb x 10(4). Before treatment SOD activity was decreased by ca. 22.5% (1.25 +/- 0.53 U/g Hb x 10(3)) while that of CAT by about 7% (5.30 +/- 1.41 U/g Hb x 10(4)), in comparison with the normal control. After treatment with the ointment, activity of both enzymes increased by about 18% to 1.55 x 10(3) U/g Hb and by about 16.5% to 6.25 x 10(4) U/g Hb, respectively. The results of our investigations showed that the ointment (containing mustard gas derivative) applied on psoriatic skin, causes increased of SOD and CAT activity in erythrocytes after regression of psoriatic lesions and treatment termination.
Kopff
M
M
Department of Biochemistry, Military Medical Academy, Lódź, Poland.
Zakrzewska
I
I
Strzelczyk
M
M
Klem
J
J
Dubiecki
W
W
D000287:Administration, Topical; D000328:Adult; D000368:Aged; D002374:Catalase / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009151:Mustard Gas / Q000737:chemistry; D009824:Ointments; D011565:Psoriasis / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013440:Sulfides / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000097:blood*
D006454:Hemoglobins; D009824:Ointments; D013440:Sulfides; C055920:2-chloroethyl-3-chloropropylsulfide; D002374:Catalase; D013482:Superoxide Dismutase; D009151:Mustard Gas