title: Type IV prepilin peptidase gene of Neisseria gonorrhoeae MS11: presence of a related gene in other piliated and nonpiliated Neisseria strains.
abstract: The assembly of type IV pili in Neisseria gonorrhoeae is a complex process likely to require the products of many genes. One of these is the enzyme prepilin peptidase, which cleaves and then N methylates the precursor pilin subunits prior to their assembly into pili. We have used a PCR amplification strategy to clone the N. gonorrhoeae prepilin peptidase gene, pilDNg. A single copy of the gene is shown to be present in the chromosome. Its product promotes correct cleavage of the gonococcal prepillin in Escherichia coli cells carrying both the prepilin peptidase gene and the pilin structural gene. PilDNg also cleaves prePulG, a type IV pilin-like protein of Klebsiella oxytoca. Moreover, PilDNg complements a mutation in the gene coding for the prepilin peptidase-like protein of K. oxytoca, pulO, partially restoring PulG-PulO-dependent extracellular secretion of the enzyme pullulanase. Finally, we show that genes homologous to pilDNg are present and expressed in a variety of species in the genus Neisseria, including some commensal strains.
authors.affiliation: Unité des Neisseria, Centre National de la Recherche Scientifique URA 1149, Institut Pasteur, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Dupuy
authors.forename: A P
authors.initials: AP
authors.lastname: Pugsley
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D010450:Endopeptidases; D004926:Escherichia coli; D010861:Fimbriae, Bacterial; D015723:Gene Library; D005798:Genes, Bacterial; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D009343:Neisseria / Q000201:enzymology / Q000235:genetics*; D009344:Neisseria gonorrhoeae / Q000201:enzymology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D007527:Isoenzymes; D010450:Endopeptidases; C067127:prepilin peptidase protein, Bacteria