Quantitative determination of fluconazole-amphotericin B antagonism to Candida albicans by agar diffusion.
Fluconazole is a bis-triazole antifungal which partly antagonizes the activity of amphotericin B against Candida albicans (and other Candida species). This antagonism can easily be demonstrated by radial agar diffusion. Amphotericin B at reservoir concentrations of up to 1.2 mg l-1 showed a diffusion behaviour which was described by means of a simple mathematical analysis. Therefore quantitative determination of the antagonism was possible. Using a reference strain of Candida albicans, the critical concentration of amphotericin B was increased by fluconazole up to 2.4-fold.
Scheven
M
M
Medizinal-, Lebensmittel- und Veterinäruntersuchungsamt, Hygieneinstitut Standort Gera, FR Germany.
Senf
L
L
D000666:Amphotericin B / Q000008:administration & dosage / Q000037:antagonists & inhibitors*; D002176:Candida albicans / Q000187:drug effects*; D015725:Fluconazole / Q000008:administration & dosage / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D008954:Models, Biological
D000666:Amphotericin B; D015725:Fluconazole