Diagnostic associations of p53 immunostaining in fine needle aspiration cytology of the breast.
Duplicate cytospin preparations were made from 46 symptomatic breast fine needle aspirates. One of each pair was assigned to benign or malignant categories by one experienced observer as part of the "triple approach" patient assessment. The other was immunostained with DO7, a monoclonal antibody to recombinant p53 protein, and rated by another observer as positive or negative for nuclear staining, unaware of the cytodiagnosis. Positive controls included carcinomas known to have mutant p53, while negative controls were of the reagent substitution type. Of the 26 aspirates with a benign cytodiagnosis (verified by the triple approach), 23 were p53 protein-negative and three positive. Of the 20 with a malignant cytodiagnosis (histologically confirmed), six were p53 protein-negative and 14 positive (exact P < 0.0001). As a diagnostic test this would give 70% sensitivity and 88% specificity.
Stephenson
T J
TJ
Department of Pathology, University of Sheffield Medical School, UK.
Royds
J A
JA
Silcocks
P B
PB
Brammer
H J
HJ
Shorthouse
A J
AJ
Underwood
J C
JC
D000911:Antibodies, Monoclonal; D001707:Biopsy, Needle; D001940:Breast / Q000737:chemistry* / Q000473:pathology*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D000911:Antibodies, Monoclonal; D016159:Tumor Suppressor Protein p53