title: [Optimized methods for measuring coagulation factors II, IV, VII and X in cats].
abstract: Since the single factor activity of the cat differed in part distinctly from humans (factor II: 94%, factor V: 500%, factor VII: 125%, factor X: 62%) the method had to be optimized, above all to exclude an influence of a reactive increased factor V-activity on the results of the other coagulation factors. Dilution series of cat pool plasma (CPP) (n = 50 cats) showed, that a commercial human placenta thromboplastin was more suitable as activating reagent than rabbit brain thromboplastin. Using the required sample predilution (1:40), measurements with the latter reagent were more precise when measured on the relation between the imprecision and the time interval between different CPP-dilution steps. Reference curves were prepared for CPP using the optimized method and human placenta thromboplastin (50 microliters 1:40 prediluted sample and 50 microliters deficient plasma were incubated at 37 degrees C for 1 minute, addition of 200 microliters Ca-thromboplastin) and reference ranges were calculated using the values of 57 cats (factor II: 74-126%, factor V: 42-184%, factor VII 56-150%, factor X: 65-143%).
authors.affiliation: Klinik für kleine Haustiere der Tierärztlichen Hochschule Hannover.
authors.forename: R
authors.initials: R
authors.lastname: Mischke
authors.forename: A
authors.initials: A
authors.lastname: Deniz
authors.forename: I
authors.initials: I
authors.lastname: Nolte
mesh_terms: D000818:Animals; D001947:Breeding; D002415:Cats / Q000097:blood*; D005165:Factor V / Q000032:analysis*; D005167:Factor VII / Q000032:analysis*; D005170:Factor X / Q000032:analysis*; D005260:Female; D008297:Male; D011516:Prothrombin / Q000032:analysis*; D012016:Reference Values
chemical_list: D005165:Factor V; D005167:Factor VII; D011516:Prothrombin; D005170:Factor X
vernacular_title: Optimierte Methode zur Messung der Gerinnungsfaktoren II, V, VII und X bei der Katze.