title: Expression of proliferation-associated proteins (proliferating cell nuclear antigen and Ki-67 antigen) in Bowen's disease.
abstract: Bowen's disease and invasive squamous cell carcinoma arising in Bowen's disease (Bowen's carcinoma) were investigated immunohistochemically with regard to their expression of proliferating cell nuclear antigen (PCNA) and the Ki-67 antigen. Both proliferation-associated proteins were found in all layers of the tumours, and were present in nuclei of any size and shape. The nucleoli lacked PCNA immunoreactivity, but they expressed the Ki-67 antigen. The PCNA labelling index was 36.9 +/- 12.6% in Bowen's disease (n = 16), and 40.7 +/- 11.0% in Bowen's carcinoma (n = 3). The Ki-67 antigen labelling index was 43.6 +/- 16.7% in Bowen's disease (n = 16), and 51.7 +/- 11.6% in Bowen's carcinoma (n = 3). Dyskeratotic cells were positive for PCNA and the Ki-67 antigen, suggesting that these cells are not in a post-mitotic state. We conclude that in dyskeratotic cells of Bowen's disease the cell cycle is interrupted by nuclear disorganization.
authors.affiliation: Department of Dermatology, University of Saarland, Homburg/Saar, Germany.
authors.forename: H P
authors.initials: HP
authors.lastname: Baum
authors.forename: I
authors.initials: I
authors.lastname: Meurer
authors.forename: G
authors.initials: G
authors.lastname: Unteregger
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D001913:Bowen's Disease / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D002467:Cell Nucleus / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D012044:Regression Analysis; D012878:Skin Neoplasms / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen