Regulatory effects of intrinsic IL-10 in IgG immune complex-induced lung injury.
IL-10 has regulatory effects in vitro on cytokine production by activated macrophages. In the IgG immune complex model of lung injury, exogenously administered IL-10 has been shown to suppress in vivo formation of TNF-alpha, up-regulation of vascular ICAM-1, neutrophil recruitment, and ensuing lung injury. In the current study, we sought to determine whether endogenous IL-10 is playing a regulatory role in the lung inflammatory response. On the basis of lung mRNA and ELISA measurements, IL-10 induction was found during development of inflammation in the IgG immune complex model of lung injury. Blocking of IL-10 by Ab resulted in a 52% increase in lung vascular permeability, a 56% increase in TNF-alpha activity in bronchoalveolar lavage fluids, and a 47 to 48% increase in bronchoalveolar lavage neutrophils and lung myeloperoxidase content. These findings suggest that IL-10 is an important natural regulator of lung inflammatory injury after deposition of IgG immune complexes.
Shanley
T P
TP
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109.
Schmal
H
H
Friedl
H P
HP
Jones
M L
ML
Ward
P A
PA
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007105:Immune Complex Diseases / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D016753:Interleukin-10 / Q000378:metabolism / Q000502:physiology*; D007958:Leukocyte Count; D008171:Lung Diseases / Q000276:immunology*; D008297:Male; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D007074:Immunoglobulin G; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009195:Peroxidase