Effect of somatolactin and related hormones on phosphate transport by flounder renal tubule primary cultures.
Winter flounder renal proximal tubule primary monolayer cultures mounted in Ussing chambers were used to determine the effect of salmon somatolactin (sSL) on transepithelial Pi and Ca2+ transport. sSL stimulated Pi reabsorption in a dose-dependent manner at physiological levels of the hormone (12.5 ng/ml). Net Pi transport was significantly altered by sSL (200 ng/ml) within 2 h after the initial exposure. Ca2+ fluxes were unchanged by the addition of 200 ng/ml sSL. The sSL-induced Pi reabsorption was abolished by 10 microM H-89, a highly specific protein kinase A inhibitor. Moreover the production and release of adenosine 3',5'-cyclic monophosphate were significantly increased after 1 and 2 h of exposure to sSL. The data indicate that sSL directly stimulates net renal Pi reabsorption by an adenosine 3',5'-cyclic monophosphate-dependent pathway. In addition to sSL, flounder SL and rat prolactin greatly, and salmon growth hormone (2.3 micrograms/ml) slightly, increased net Pi reabsorptive flux, whereas salmon prolactin had no effect.
Lu
M
M
Department of Physiology and Neurobiology, University of Connecticut, Storrs 06269.
Swanson
P
P
Renfro
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D029941:Fish Proteins; D005432:Flounder; D006023:Glycoproteins / Q000235:genetics / Q000494:pharmacology*; D013006:Growth Hormone / Q000235:genetics / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects; D007546:Isoquinolines / Q000494:pharmacology; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism*; D010907:Pituitary Hormones / Q000235:genetics / Q000494:pharmacology*; D011388:Prolactin / Q000235:genetics / Q000494:pharmacology; D051381:Rats; D012474:Salmon; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013449:Sulfonamides
D029941:Fish Proteins; D006023:Glycoproteins; D007546:Isoquinolines; D010710:Phosphates; D010907:Pituitary Hormones; D013449:Sulfonamides; C064448:somatolactin protein, fish; D011388:Prolactin; D013006:Growth Hormone; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; D002118:Calcium