Simultaneous measurement of ciliary beating and intracellular calcium.
A novel system for measuring, simultaneously, ciliary beating and intracellular free calcium is presented. The advantages and dynamic nature of the system are demonstrated by measuring the effects of the calcium ionophore lonomycin and of extracellular ATP on ciliated rabbit trachea. The results are discussed with regard to the ciliary and calcium stimulation.
Korngreen
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Department of Chemistry, Ben-Gurion University of Negev, Beer-Sheva, Israel.
Priel
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D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002923:Cilia / Q000187:drug effects / Q000502:physiology*; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D015759:Ionomycin / Q000494:pharmacology; D007700:Kinetics; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D011817:Rabbits; D013997:Time Factors; D014132:Trachea / Q000502:physiology*
D005456:Fluorescent Dyes; C049925:fura-2-am; D015759:Ionomycin; D000255:Adenosine Triphosphate; D002118:Calcium; D016257:Fura-2