Cloning of rat interleukin-13 (IL-13) cDNA and analysis of IL-13 gene expression in experimental glomerulonephritis.
Using a reverse transcriptase-polymerase chain reaction method, a cDNA for the complete coding region and part of the 5'-untranslated region of rat IL-13 was cloned from rat renal cortex RNA following the induction of anti-glomerular basement membrane antibody-induced glomerulonephritis. The coding region of IL-13 cDNA displays 74% and 87% sequence identity with the coding regions of human and mouse IL-13 cDNA, respectively. The deduced amino acid sequence of rat IL-13 reveals 63% and 79% homology with the human and mouse proteins, respectively. Using the rat IL-13 cDNA as a molecular probe, increased production of IL-13 mRNA in renal and splenic tissue is demonstrated in the first 48 hours of antibody-induced glomerulonephritis in the rat. The data suggest a role for IL-13 in the inflammatory response in vivo.
Lakkis
F G
FG
Renal Division, Emory University School of Medicine, Atlanta, Georgia.
Cruet
E N
EN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000302:isolation & purification; D005786:Gene Expression Regulation; D005921:Glomerulonephritis / Q000378:metabolism*; D006801:Humans; D018793:Interleukin-13; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics*; D007678:Kidney Glomerulus / Q000378:metabolism*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D018793:Interleukin-13; D007378:Interleukins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger