Direct influence of melatonin on steroid, nonapeptide hormones, and cyclic nucleotide secretion by granulosa cells isolated from porcine ovaries.
The release of progesterone, estradiol-17 beta, oxytocin, arginine-vasopressin, cAMP, and cGMP by cultured granulosa cells isolated from porcine ovaries without and in the presence of melatonin (0.001, 0.01, 0.1, 1, 10, and 100 ng/ml medium) was analyzed. It was found that melatonin is able to inhibit progesterone and stimulate estradiol secretion. Melatonin treatments significantly inhibited oxytocin release. Some inhibition of vasopressin and cAMP and significant stimulation of cGMP also resulted from melatonin treatment. The present observations suggest a direct effect of melatonin on the steroid, nonapeptide hormone, and cyclic nucleotide release from porcine ovarian cells.
Sirotkin
A V
AV
Research Institute of Animal Production, Nitra, Slovakia.
D000818:Animals; D001120:Arginine / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006728:Hormones / Q000378:metabolism*; D008550:Melatonin / Q000494:pharmacology*; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D010121:Oxytocin / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D013552:Swine
D006728:Hormones; D009712:Nucleotides, Cyclic; D011374:Progesterone; D004958:Estradiol; D010121:Oxytocin; D001120:Arginine; D000242:Cyclic AMP; D006152:Cyclic GMP; D008550:Melatonin