Catabolite regulation of Bacillus subtilis acetate and acetoin utilization genes by CcpA.
The Bacillus subtilis acsA (acetyl coenzyme A synthetase) and acuABC (acetoin utilization) genes were previously identified in the region downstream from the ccpA gene, which encodes a protein required for catabolite repression of the amyE (alpha-amylase) gene. The acsA and acuABC genes are divergently transcribed, with only 20 bp separating the -35 sequences of their promoters. Expression of these genes was maximal in stationary phase and was repressed by the addition of glucose to the growth medium. Two sites resembling amyO, the cis-acting regulatory target site for amyE, were identified in the acsA and acuABC promoter regions. Glucose repression of acsA and acuABC transcription was dependent on both CcpA and the amyO-like sequences.
Grundy
F J
FJ
Department of Biochemistry and Molecular Biology, Albany Medical College, New York 12208.
Turinsky
A J
AJ
Henkin
T M
TM
D000106:Acetate-CoA Ligase / Q000235:genetics; D000085:Acetates / Q000378:metabolism*; D000093:Acetoin / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D004794:Enzyme Repression / Q000187:drug effects; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D005947:Glucose / Q000494:pharmacology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins / Q000235:genetics*
D000085:Acetates; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C069286:catabolite control proteins, bacteria; D000093:Acetoin; D000106:Acetate-CoA Ligase; D005947:Glucose