Effects of various growth factors and histamine on cultured keloid fibroblasts.
OBJECTIVE
We investigated the effects of several growth factors on [3H]thymidine incorporation and procollagen type I carboxyterminal propeptide (P1CP) production, which reflects type I collagen metabolism, in keloid and normal fibroblasts.


RESULTS
Six fibroblast cell strains, derived from keloid or normal skin, exhibited similar growth responses to platelet-derived growth factor, transforming growth factor beta 1 (TGF-beta 1), gamma-interferon (gamma-IFN) and histamine. In contrast, keloid fibroblasts showed significantly greater growth response to epidermal growth factor (EGF) than normal fibroblasts. P1CP production was 4.4 times higher in 6 strains of keloid fibroblasts than in 6 controls. Treatment with gamma-IFN (100 U/ml) decreased P1CP production in both groups; the effect was significantly greater in keloid fibroblasts. TGF-beta 1 treatment upregulated P1CP production in both groups. Treatment with histamine increased P1CP production in keloid fibroblasts, although it did not change that in the controls.


CONCLUSIONS
EGF and histamine may play some role in the development of keloids.
Kikuchi
K
K
Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
Kadono
T
T
Takehara
K
K
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D006133:Growth Substances / Q000494:pharmacology*; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007627:Keloid / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D010446:Peptide Fragments / Q000096:biosynthesis*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011347:Procollagen / Q000096:biosynthesis*; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D006133:Growth Substances; D010446:Peptide Fragments; D010982:Platelet-Derived Growth Factor; D011347:Procollagen; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C042813:procollagen type I carboxy terminal peptide; D004815:Epidermal Growth Factor; D006632:Histamine; D007371:Interferon-gamma; D003094:Collagen