[Lipid peroxidation parameters and activities of lysosomal enzymes in patients with diabetes mellitus].
The processes of lipid peroxidation and activities of lysosomal enzymes were studied in 56 patients with type I and II diabetes mellitus. The rate of lipid peroxidation of red cell membranes was assessed from the activities of enzymatic (NADPH-dependent) and nonenzymatic (ascorbate-dependent) lipid peroxidation, from accumulation of acylhydroperoxides, intermolecular joints, and from spontaneous red cell hemolysis. Activities of lysosomal enzymes (cathepsins, acid DNAse, and beta-galactosidase) were measured in leukoconcentrate. The activity of enzymatic system of lipid peroxidation and acylhydroperoxide content in red cell membranes were found increased. In parallel with this, a deficiency in leukocytic lysosomes of beta-galactosidase and DNAse was revealed. The detected metabolic disturbances may be regarded as one of the pathogenetic mechanisms of development of diabetic angiopathies. A relationship was revealed between changes in lipid peroxidation parameters and activities of lysosomal enzymes, on the one hand, and diabetes mellitus type and duration, on the other.
Chugunova
L G
LG
Dubinina
I I
II
D000293:Adolescent; D000328:Adult; D000368:Aged; D002403:Cathepsins / Q000097:blood; D003851:Deoxyribonucleases / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000201:enzymology*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology*; D004910:Erythrocyte Membrane / Q000502:physiology; D006801:Humans; D007962:Leukocytes / Q000201:enzymology / Q000648:ultrastructure; D015227:Lipid Peroxidation / Q000502:physiology*; D008247:Lysosomes / Q000201:enzymology*; D008875:Middle Aged; D001616:beta-Galactosidase / Q000097:blood
D003851:Deoxyribonucleases; D001616:beta-Galactosidase; D002403:Cathepsins
Pokazateli perekisnogo okisleniia lipidov i aktivnost' lizosomal'nykh fermentov u bol'nykh sakharnym diabetom.