[Gene expression of TGF alpha, TGF beta 1 and C-erbB2 oncogene in human pancreatic carcinoma cell lines].
Our previous studies have shown that 3 human pancreatic carcinoma cell lines (PC-1, PC-2, PC-3) established in our laboratory express EGF receptor gene, EGF and its mRNA. In this study, gene expression of TGF alpha (a ligand of EGF receptor), TGF beta 1 and c-erbB2 oncogene were studied. The results showed that all 3 human pancreatic carcinoma cell lines expressed TGF alpha mRNA. PC-2, PC-3 cell lines also express TGF beta 1 mRNA. C-erbB2 oncogene (a homology of EGF receptor) mRNA was strongly expressed in PC-1, weakly in PC-2 and was not detectable in PC-3. The presence of TGF alpha and c-erbB2 in human pancreatic carcinoma cell lines further verifies the occurrence of an autocrine growth regulation of this cancer.
Chen
J
J
Peking Union Medical College Hospital, Beijing.
D066246:ErbB Receptors / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D018719:Erb-b2 Receptor Tyrosine Kinases; D016211:Transforming Growth Factor alpha / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014407:Tumor Cells, Cultured
D066246:ErbB Receptors; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D016211:Transforming Growth Factor alpha; D016212:Transforming Growth Factor beta