Hybrid liver support system in a short term application on hepatectomized pigs.
A short term application of a hybrid liver support system in circuits with continuous plasma-separation was investigated in a model of hepatectomized pigs under general anesthesia. Primary pig hepatocytes were immobilized in a bioreactor with three independent capillary systems. An immune barrier is achieved by avoiding the direct contact of blood cells with the hepatocytes by a plasmaseparation step and by an outflow filtration within the reactor. In three groups (hepatectomized pigs and system with- or without hepatocytes as well as untreated pigs with system without hepatocytes), the short term metabolism of the reactors was positively demonstrated by investigating ammonia detoxification, phenylalanine- and lactate metabolism. Limitations of the presented model are discussed.
Gerlach
J
J
Chirurgische Klinik, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, Germany.
Trost
T
T
Ryan
C J
CJ
Meissler
M
M
Hole
O
O
Müller
C
C
Neuhaus
P
P
D000641:Ammonia / Q000378:metabolism / Q000506:poisoning; D000818:Animals; D001187:Artificial Organs; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D006440:Hemofiltration; D006498:Hepatectomy; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000098:blood supply / Q000166:cytology / Q000502:physiology; D008102:Liver Circulation / Q000502:physiology*; D008297:Male; D010649:Phenylalanine / Q000378:metabolism; D013552:Swine; D014508:Urea / Q000378:metabolism
D007773:Lactates; D010649:Phenylalanine; D000641:Ammonia; D014508:Urea