title: Conformational changes of DNA minicircles upon the binding of the archaebacterial histone-like protein MC1.
abstract: Binding of the archaebacterial histone-like protein MC1 to DNA minicircles has been examined by gel retardation and electron microscopy. MC1 preferentially binds to a 207-base pair relaxed DNA minicircle as compared with the linear fragment. Random binding is observed at very low ionic strength, and a slight increase in salt concentration highly favors the formation of a complex that corresponds to the binding of two MC1 molecules per DNA ring. Measurements of dissociation rates show that this complex is remarkably stable, and electron microscopy reveals that it is characterized by two diametrically opposed kinks. These results are discussed in regard to the mechanisms by which MC1 affects DNA structure.
authors.affiliation: Centre de Biophysique Moléculaire, Orléans, France.
authors.forename: F
authors.initials: F
authors.lastname: Toulmé
authors.forename: E
authors.initials: E
authors.lastname: Le Cam
authors.forename: C
authors.initials: C
authors.lastname: Teyssier
authors.forename: E
authors.initials: E
authors.lastname: Delain
authors.forename: P
authors.initials: P
authors.lastname: Sautière
authors.forename: J C
authors.initials: JC
authors.lastname: Maurizot
authors.forename: F
authors.initials: F
authors.lastname: Culard
mesh_terms: D019843:Archaeal Proteins; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D002850:Chromatography, Gel; D004270:DNA, Circular / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000378:metabolism; D007700:Kinetics; D015636:Magnesium Chloride / Q000494:pharmacology; D017020:Methanosarcina / Q000378:metabolism*; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D011485:Protein Binding; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D019843:Archaeal Proteins; D001426:Bacterial Proteins; D004270:DNA, Circular; C051211:HMb protein, Methanosarcina; D006657:Histones; D012261:Ribonucleoproteins; D015636:Magnesium Chloride; D012965:Sodium Chloride