[Measurement of free intracellular Ca2+ concentration in cultivated hippocampal neurons].
The cascade of pathobiochemical reactions caused by cerebral ischemia is complex and not completely understood. One important factor seems to be the disturbed calcium homeostasis. Measurement of cytosolic-free Ca2+ concentration [Ca2+]i is possible with the Ca(2+)-sensitive dye fura-2. Using this technique it can be investigated if, and how, neuroprotective drugs influence the cellular calcium homeostasis. In hippocampal neurons K+ or glutamate, added to the buffer solution, evoked elevations of [Ca2+]i in a concentration-dependent manner. We demonstrated that the calcium antagonist nimodipine or the non-competitive NMDA receptor antagonist dizocilpine added to the buffer solution could inhibit concentration-dependently the elevation of [Ca2+]i. The measurement of [Ca2+]i can be used for studies on the mechanism of action as well as for screening purposes of neuroprotective drugs.
Hörster
D
D
Institut für Pharmakologie und Toxikologie im Fachbereich Pharmazie und Lebensmittelchemie, Philipps-Universität Marburg.
Krieglstein
J
J
D000818:Animals; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002478:Cells, Cultured; D016257:Fura-2; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006624:Hippocampus / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000494:pharmacology; D009553:Nimodipine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D013268:Stimulation, Chemical
D005971:Glutamates; D018377:Neurotransmitter Agents; D018698:Glutamic Acid; D009553:Nimodipine; D016202:N-Methylaspartate; D011188:Potassium; D002118:Calcium; D016257:Fura-2
Messung der freien intrazellulären Ca(2+)-Konzentration in kultivierten hippocampalen Neuronen.