Vitamin E, glutathione S-transferase and gamma-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens.
1. The effects of alpha-tocopherol and gamma-tocotrienol on glutathione S-transferase (GST) and gamma-glutamyl transpeptidase (gamma-GT) activities in cultured hepatocytes prepared from rats treated with diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) were investigated. 2. Both the alpha-tocopherol and gamma-tocotrienol treated hepatocytes showed significantly higher (P < 0.05) GST activities than untreated hepatocytes prepared from the carcinogen treated rats in the first 3 days of culture. Treatment with alpha-tocopherol and gamma-tocotrienol generally resulted in a tendency to increase the GST activities above that in the untreated hepatocytes. 3. Treatment with high doses (125-250 microM) of alpha-tocopherol and low doses (12.5-25 microM) of gamma-tocotrienol generally resulted in a significant reduction in gamma-GT activities at 1-3 days. gamma-GT activities are reduced as the dose of alpha-tocopherol and gamma-tocotrienol are increased.
Ong
F B
FB
Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur.
Wan Ngah
W Z
WZ
Top
A G
AG
Khalid
B A
BA
Shamaan
N A
NA
D015073:2-Acetylaminofluorene / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002478:Cells, Cultured; D004052:Diethylnitrosamine / Q000633:toxicity; D004347:Drug Interactions; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D024508:Tocotrienols; D014810:Vitamin E / Q000031:analogs & derivatives / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D002273:Carcinogens; D024508:Tocotrienols; D014810:Vitamin E; D004052:Diethylnitrosamine; D015073:2-Acetylaminofluorene; C082032:tocotrienol, alpha; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase; D013936:Thymidine