title: Growth dynamics of pancreatic islet cell populations during fetal and neonatal development of the rat.
abstract: Rats from 18 days fetal to 28 days neonatal ages were studied for the total population sizes and cell proliferation activities of insulin secreting B cells, glucagon secreting A cells, somatostatin secreting D cells, and pancreatic polypeptide secreting PP cells. Cell population sizes were assessed by morphometric quantitation of immunohistochemically stained cells by a linear scanning method and cell proliferation activities were estimated by [3H]-thymidine labeling indices of these cell populations. There was a continuous increase in population sizes for all 4 islet cell types, with the fastest increase occurring in the last 4 days of gestation. The accelerated growth of these islet cell populations during late gestation was accomplished by a high cell proliferative activity at 20-22 days of gestation and a large influx of undifferentiated epithelial cells differentiating into the specific islet cell populations during this period. There was a reduction of population growth and cell proliferation for all islet cell types during the first 3-4 days of life. Growth activities continued for all islet cell populations after the 4th postnatal day, with a renewed acceleration in growth activities for B and A cells at this time. After the 10th neonatal day, the cell proliferation and total population growth continued at slow rates for all 4 islet cell types. The contribution from undifferentiated epithelial cells into the specific islet cell populations was negligible for B and A cells but continued at a low rate for PP and D cells during the first 10 days after birth. For B and A cell populations, there was a possibility that some cell loss occurred during the first 10 days of neonatal life. These dynamic changes of the growth characteristics provide a basis for understanding the abnormal growth of the endocrine pancreas.
authors.affiliation: Department of Pediatrics, Case Western University, Cleveland, Ohio 44106.
authors.forename: H L
authors.initials: HL
authors.lastname: Kaung
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D016923:Cell Death; D002454:Cell Differentiation; D002455:Cell Division; D048429:Cell Size; D005314:Embryonic and Fetal Development / Q000502:physiology; D004847:Epithelial Cells; D005260:Female; D007515:Islets of Langerhans / Q000166:cytology* / Q000196:embryology*; D008297:Male; D009024:Morphogenesis; D010179:Pancreas / Q000166:cytology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley