title: Blood volume determination with sodium fluorescein and radioactive chromium--a clinical comparison of methods.
abstract: OBJECTIVE
  There exists no method so far for the determination of circulating blood volume as an important parameter of circulatory function widely usable under clinical conditions. Therefore, the present study was designed to investigate whether identical distribution spaces could be measured by two methods for blood volume determination using sodium fluorescein (SoF) and radioactively labelled red blood cells (51Cr*).
  
  
  METHODS
  Comparative study.
  
  
  METHODS
  Operating theatre, recovery room, or intensive care unit of a university hospital.
  
  
  METHODS
  35 patients undergoing abdominal, urological or vascular surgery.
  
  
  METHODS
  Simultaneous determinations of blood volume using SoF and 51Cr* in the intra- and postoperative period.
  
  
  RESULTS
  There were no significant differences between the calculated means of blood volume (4,445 vs. 4,407 ml), red cell volume (1,554 vs. 1,540 ml), and plasma volume (2,891 vs. 2,807 ml) for 51Cr*-vs. SoF-stained red blood cells. The coefficient of correlation between the two methods was r = 0.95. The mean percentage error was -0.6% between the two methods, the precision 5.6%.
  
  
  CONCLUSIONS
  SoF-stained erythrocytes allow a determination of the same distribution space as the well-established radioactive method using 51Cr*. Therefore, SoF-staining may replace 51Cr* labelling of red blood cells for the determination of blood volume in patients.
authors.affiliation: Abteilung für Anästhesiologie, Medizinische Hochschule, Hannover.
authors.forename: I
authors.initials: I
authors.lastname: Lauermann
authors.forename: G
authors.initials: G
authors.lastname: Wilhelm
authors.forename: E
authors.initials: E
authors.lastname: Kirchner
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001810:Blood Volume / Q000502:physiology*; D002860:Chromium Radioisotopes; D004909:Erythrocyte Indices; D004912:Erythrocytes; D005260:Female; D019793:Fluorescein; D005452:Fluoresceins; D006801:Humans; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D011237:Predictive Value of Tests; D012016:Reference Values
chemical_list: D002860:Chromium Radioisotopes; D005452:Fluoresceins; D019793:Fluorescein