title: Evidence for the expression of dynorphin gene in the prolactin-immunoreactive neurons of the rat lateral hypothalamus.
abstract: Dynorphin B (DYN B) immunoreactivity was recently reported in a population of prolactin (PRL)-immunoreactive neurons of the rat lateral hypothalamus (LH). By coupling immunocytochemistry and in situ hybridization using two synthetic oligonucleotide probes complementary to DYN mRNA, a hybridization signal was observed over the neurons of the LH exhibiting both DYN- and PRL-like immunoreactivities. Our results clearly demonstrate that these neurons contain the mRNA encoding preproDYN and are able to synthesize authentic DYN B in colocalization with a peptide related to PRL.
authors.affiliation: CNRS UA 561, Laboratoire d'Histologie, Embryologie, Cytogénétique, Faculté de Médecine, Besançon, France.
authors.forename: B
authors.initials: B
authors.lastname: Griffond
authors.forename: C
authors.initials: C
authors.lastname: Colard
authors.forename: A
authors.initials: A
authors.lastname: Deray
authors.forename: D
authors.initials: D
authors.lastname: Fellmann
authors.forename: C
authors.initials: C
authors.lastname: Bugnon
mesh_terms: D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D004399:Dynorphins / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics; D004723:Endorphins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000502:physiology*; D006651:Histocytochemistry; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D015345:Oligonucleotide Probes; D011388:Prolactin / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats
chemical_list: D004723:Endorphins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004399:Dynorphins; C036200:rimorphin; D011388:Prolactin