[Effect of prednisolon, vincristine and mitoxantrone on glucocorticoid receptor in HL-60 cell line].
The effects of prednisolon, vincristine and Mitoxantrone on glucocorticoid receptor (GCR) content and affinity were examined in HL-60 cell line with a whole-cell assay in vitro. The fall in GCR number was observed following a short time exposure to prednisolon, vincristine or Mitoxantrone alone. The significant decrease occurred in a dose-dependent (10(-8)-10(-6) mol/L) and time-dependent manner. The treatment of HL-60 cells with 10(-6) mol/L led to a decrease in the affinity of whole cell GCR for [3H]Dex, but vincristine had no that effect. Synergia in GCR content was found during the treatment of HL-60 cells with prednisolon plus Mitoxantrone or prednisolon plus vincristine. The significance of these effects is discussed.
Cai
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S
Chen
J
J
Ye
S
S
Yu
F
F
D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D008942:Mitoxantrone / Q000494:pharmacology*; D011239:Prednisolone / Q000494:pharmacology*; D011965:Receptors, Glucocorticoid / Q000187:drug effects*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014750:Vincristine / Q000494:pharmacology*
D011965:Receptors, Glucocorticoid; D014750:Vincristine; D011239:Prednisolone; D008942:Mitoxantrone