PRE3, highly homologous to the human major histocompatibility complex-linked LMP2 (RING12) gene, codes for a yeast proteasome subunit necessary for the peptidylglutamyl-peptide hydrolyzing activity.
20S proteasomes are multifunctional proteinase complexes ubiquitous in eucaryotes. We have cloned the yeast PRE3 gene by complementation of the pre3-2 mutation, which leads to a defect in the peptidylglutamyl-peptide hydrolyzing activity of the 20S proteasome. The PRE3 gene, a beta-type member of the proteasomal gene family, is essential for cellular life and codes for a 193-amino acid proteasomal subunit with a predicted molecular mass of 21.2 kDa. The Pre3 protein shows striking homology to the human proteasome subunits Hs delta and Lmp2 (Ring12). Lmp2 is encoded in the major histocompatibility complex class II region implicating proteasomes in antigen processing.
Enenkel
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Institut für Biochemie, Universität Stuttgart Pfaffenwaldring, Germany.
Lehmann
H
H
Kipper
J
J
Gückel
R
R
Hilt
W
W
Wolf
D H
DH
D000595:Amino Acid Sequence; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D010450:Endopeptidases; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006801:Humans; D006868:Hydrolysis; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D011506:Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
D005656:Fungal Proteins; D005971:Glutamates; D009097:Multienzyme Complexes; D010455:Peptides; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; C071313:LMP-2 protein; D018698:Glutamic Acid; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C086455:PRE3 protein, S cerevisiae; D046988:Proteasome Endopeptidase Complex