Effect of macrolide antibiotics on ciliary motility in rabbit airway epithelium in-vitro.
We have studied ciliary beat frequency (CBF) of rabbit cultured tracheal epithelium by a photoelectric method in-vitro. Addition of erythromycin and roxithromycin increased CBF in a dose-dependent fashion, whereas clarithromycin was without effect. The rank order potency of macrolide was roxithromycin > erythromycin >> clarithromycin. The roxithromycin-induced increase in CBF was not altered by propranolol, AA-861, or verapamil, but partially attenuated by indomethacin. Roxithromycin increased intracellular cAMP concentrations. These results suggest that certain macrolides can stimulate airway ciliary motility probably via prostaglandin- and cAMP-dependent regulatory pathways, which may affect mucociliary transport function in the respiratory tract.
Takeyama
K
K
First Department of Medicine, Tokyo Women's Medical College, Japan.
Tamaoki
J
J
Chiyotani
A
A
Tagaya
E
E
Konno
K
K
D000818:Animals; D002923:Cilia / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D004594:Electrophysiology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004917:Erythromycin / Q000494:pharmacology*; D066298:In Vitro Techniques; D011817:Rabbits; D015575:Roxithromycin / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*
D015575:Roxithromycin; D004917:Erythromycin; D000242:Cyclic AMP