Wild type Rhizobium etli, a bean symbiont, produces acetyl-fucosylated, N-methylated, and carbamoylated nodulation factors.
Phaseolus vulgaris (common bean) can be nodulated by different Rhizobium species. A new species has been recently proposed: Rhizobium etli. Following transcriptional activation of the bacterial nodulation genes using naringenin or bean seed exudate, we have isolated, purified, and characterized R. etli extracellular nodulation factors. They are chitopentameric compounds that are N-methyl-N-vaccenoylated at their non-reducing end. At position 6 of the reducing N-acetyl-D-glucosamine, they are 4-O-acetyl-L-fucosylated. Minor compounds bear a carbamate group on the terminal non-reducing saccharidic residue.
Poupot
R
R
Laboratoire de Pharmacologie et Toxicologie Fondamentales, Centre National de la Recherche Scientifique, Toulouse, France.
Martinez-Romero
E
E
Gautier
N
N
Promé
J C
JC
D000117:Acetylglucosamine / Q000032:analysis / Q000378:metabolism; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000382:microbiology; D005643:Fucose / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D005798:Genes, Bacterial; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal; D012231:Rhizobium / Q000235:genetics / Q000378:metabolism*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013559:Symbiosis
D008070:Lipopolysaccharides; C092533:Nod factor, Rhizobium etli; D009844:Oligosaccharides; D005643:Fucose; D000117:Acetylglucosamine