Juvenile hormone action to suppress gene transcription and influence message stability.
Proteins normally expressed in high abundance only at larval-pupal metamorphosis in Trichoplusia ni were examined in a comparative analysis of the role and level of hormonal control of their expression. Some related proteins in the hemocyanin-superfamily (i.e., an acidic protein [AJHSP1] and two basic proteins [BJHSP1, BJHSP2]) were shown by nuclear run-on analysis to be specifically transcriptionally suppressed by juvenile hormone (JH), while transcription of another member of that family which is also metamorphosis-associated (arylphorin) was not specifically sensitive to JH. The stability of the mRNA for those members transcriptionally down-regulated by JH appeared to decrease under high JH conditions. While each protein was resorbed to some extent by the prepupal fat body, only the two basic proteins were quantitatively cleared from prepupal hemolymph. The JH-sensitive proteins studied appear to be encoded in single copy genes not immediately juxtaposed in the genome. These and previous studies now permit a more comprehensive understanding of the different combinations of mechanisms involving transcription, mRNA stability, translation, and protein clearance that operate to regulate these metamorphosis-associated proteins.
Jones
G
G
School of Biological Sciences, University of Kentucky, Lexington 40506.
Venkataraman
V
V
Manczak
M
M
Schelling
D
D
D000818:Animals; D002219:Carbamates / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005216:Fat Body / Q000254:growth & development / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D017344:Genes, Insect; D006023:Glycoproteins; D006433:Hemocyanins / Q000235:genetics; D006458:Hemolymph / Q000737:chemistry; D007301:Insect Hormones / Q000096:biosynthesis / Q000235:genetics; D019476:Insect Proteins; D007605:Juvenile Hormones / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D007814:Larva; D008675:Metamorphosis, Biological / Q000235:genetics; D009036:Moths / Q000235:genetics* / Q000254:growth & development; D005810:Multigene Family; D048448:Phenylcarbamates; D011061:Poly A / Q000096:biosynthesis; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D002219:Carbamates; D006023:Glycoproteins; D007301:Insect Hormones; D019476:Insect Proteins; D007605:Juvenile Hormones; D048448:Phenylcarbamates; D011506:Proteins; D012333:RNA, Messenger; C083992:basic juvenile hormone suppressible protein-1, insect; C083993:basic juvenile-hormone suppressible protein-2, insect; C041137:storage proteins, Insecta; D011061:Poly A; D006433:Hemocyanins; C052034:fenoxycarb