Evaluation of an enzyme immunoassay kit for the detection of Clostridium difficile enterotoxin.
The Premier Clostridium difficile toxin A enzyme immunoassay (EIA) kit was evaluated for the detection of C. difficile enterotoxin in fecal samples. A total of 314 samples was tested by culture, cytotoxin detection and EIA kit. Compared to a combined culture/cytotoxin result the Premier EIA kit had a sensitivity of 88.3%, a specificity of 100%, a predictive value positive of 100% and a predictive value negative of 87.4%. Test results were available within 3 hrs providing a rapid and reliable means of detecting C. difficile enterotoxin.
Bowman
R A
RA
Department of Microbiology, University of Western Australia, Nedlands.
Arrow
S
S
Croese
L
L
Riley
T V
TV
D016360:Clostridioides difficile / Q000737:chemistry*; D004768:Enterotoxins / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D004768:Enterotoxins; D011933:Reagent Kits, Diagnostic