title: Radiosensitive populations and recovery in X-ray-induced apoptosis in the developing cerebellum.
abstract: Sprague-Dawley rats received a single dose of 2 Gy X-rays at the age of 1 or 3 days and were killed at different intervals. Dying cells with the morphological characteristics of apoptosis appeared in the external and internal granular layers (EGL and IGL) and white matter (WM) of the cerebellum, mainly 3-6 h after irradiation, and decreased thereafter to reach normal values between 48 h and 5 days later. This process was curbed by the injection of cycloheximide at a dose of 1 microgram/g body weight. In addition, the number of mitoses in EGL rapidly decreased after irradiation and did not reach normal values until a few days later. Proliferating cell nuclear antigen (PCNA)-immunoreactive cells, which were chiefly found in EGL but also in IGL and WM, dramatically decreased in number from 3 to 48 h after irradiation. PCNA-immunoreactive cells reappeared and reached age-matched values in the following days. Hu (considered as an early neuronal marker) and vimentin immunocytochemistry disclosed that Hu-nonreactive cells in the upper level of EGL, Hu-immunoreactive cells in the inner level of EGL, Bergmann glia and many astrocytes in WM, as well as many non-typified cells in WM, were radiosensitive populations, whereas Purkinje cells were not. The present results indicate that irradiation at P1 or P3 blocks mitosis in EGL and kills sensitive cells mainly in the late G1 and S phases of the cell cycle, probably by apoptosis through a protein synthesis-mediated process. Radiosensitive cells are germinal cells and neuroblasts in EGL, Bergmann glia, astrocytes in WM, and non-typified cells, probably glial cell precursors, in WM. Surviving cells in EGL and PCNA-immunoreactive cells in other cortical layers and white matter reconstitute the cerebellum following a single dose of X-rays.
authors.affiliation: Unidad de Neuropatología, Hospital Príncipes de España, Universidad de Barcelona, Hospitalet de Llobregat.
authors.forename: I
authors.initials: I
authors.lastname: Ferrer
authors.forename: T
authors.initials: T
authors.lastname: Serrano
authors.forename: R
authors.initials: R
authors.lastname: Rivera
authors.forename: M
authors.initials: M
authors.lastname: Olivé
authors.forename: M J
authors.initials: MJ
authors.lastname: Zújar
authors.forename: F
authors.initials: F
authors.lastname: Graus
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D015415:Biomarkers; D002453:Cell Cycle / Q000528:radiation effects; D002526:Cerebellar Diseases / Q000209:etiology / Q000473:pathology; D002531:Cerebellum / Q000254:growth & development / Q000473:pathology* / Q000528:radiation effects*; D002540:Cerebral Cortex / Q000254:growth & development / Q000473:pathology / Q000528:radiation effects; D005260:Female; D008297:Male; D009687:Nuclear Proteins / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen