title: Dengue virus-specific memory T cell responses in human volunteers receiving a live attenuated dengue virus type 2 candidate vaccine.
abstract: A live attenuated dengue virus type 2 candidate vaccine (16681-PDK53) was evaluated in a phase I trial in 10 nonimmune adult volunteers. The dengue virus-specific memory T cell responses were analyzed as part of this study. Dengue virus-specific T cell proliferative responses were observed in all subjects after stimulating their peripheral blood mononuclear cells with live viruses or noninfectious viral antigens. The highest proliferative response was against dengue virus type 2, although cross-reactivity with other flaviviruses was detected to a lesser degree in some subjects. Dengue virus type 2-specific CD4+ and CD8+ cytotoxic T lymphocytes were generated in all vaccinees. This study investigated whether the candidate vaccine was efficacious in inducing dengue virus-specific CD4+ and CD8+ T cell memory after a single immunization in nonimmune recipients.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: T
authors.initials: T
authors.lastname: Dharakul
authors.forename: I
authors.initials: I
authors.lastname: Kurane
authors.forename: N
authors.initials: N
authors.lastname: Bhamarapravati
authors.forename: S
authors.initials: S
authors.lastname: Yoksan
authors.forename: D W
authors.initials: DW
authors.lastname: Vaughn
authors.forename: C H
authors.initials: CH
authors.lastname: Hoke
authors.forename: F A
authors.initials: FA
authors.lastname: Ennis
mesh_terms: D000328:Adult; D015704:CD4 Antigens; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens; D003715:Dengue / Q000276:immunology* / Q000517:prevention & control; D003716:Dengue Virus / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D009000:Monocytes / Q000276:immunology; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D014613:Vaccines, Attenuated; D014765:Viral Vaccines