title: Giant cell tumor of bone: analysis of proliferative cells by double-labeling immunohistochemistry with anti-proliferating cell nuclear antigen antibody and culture procedure.
abstract: Giant cell tumor of bone (GCT) consists of multinucleated giant cells, fibroblast-like cells, and macrophage-like cells, but what constitutes the principal neoplastic cells and their histogenesis remains controversial. Proliferating cells in GCT have been immunophenotypically characterized with the cell cycle-related antigen proliferating cell nuclear antigen (PCNA). In seven cases, PCNA monoclonal antibody was applied by double-labeling immunohistochemical methods together with other cell-specific monoclonal antibodies. Many proliferating PCNA-positive cells were found in the vimentin-positive fibroblast-like cells, while almost all macrophage-like cells were negative for PCNA. The study of cultured cells showed that the fibroblast-like spindle-shaped cells were a neoplastic element. In addition, osteoclast-like cells were detected in co-culture systems of cells cultured from GCT with spleen cells. These studies support the concept that GCT is seen as a neoplasm of fibroblast-like cells with a strong reactive infiltration of cells from the mononuclear phagocyte system.
authors.affiliation: Department of Orthopaedic Surgery, Kumamoto University School of Medicine.
authors.forename: Y
authors.initials: Y
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Yonemura
authors.forename: K
authors.initials: K
authors.lastname: Nishida
authors.forename: K
authors.initials: K
authors.lastname: Takagi
mesh_terms: D000911:Antibodies, Monoclonal; D001859:Bone Neoplasms / Q000473:pathology*; D002455:Cell Division; D018212:Giant Cell Tumor of Bone / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D009687:Nuclear Proteins / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen