[The isolation and genetic characteristics of the new cell line of mouse myeloma spEBR-5 selected for ethidium bromide resistance and characterized by multiple drug resistance].
Stable mutant cells spEBR-5, resistant to ethidium bromide in concentration of 5 micrograms/ml, have been isolated by multistep selection in mouse myeloma cells sp2/0-Ag14. The spEBR-5 cells, selected with ethidium bromide, appeared to be cross resistant to unrelated drugs in connection with mdr/lb gene amplification and overexpression. Five specific chromosomal markers were detected in the karyotype of spEBR-5 cells as a result of chromosome structural rearrangement. No cytological manifestation of gene amplification such as HCR of chromosomes or DMs was found. A diffuse location of amplified sequences in chromosome(s) is suggested. The new mutant cell lines spEBR-5 can be used as a model for investigation of multidrug resistance mechanisms.
Berezkina
E V
EV
Lipskaia
L A
LA
Grinchuk
T M
TM
Kovaleva
Z V
ZV
Sorokina
E A
EA
Vasiukhin
V I
VI
Ignatova
T N
TN
D000818:Animals; D002469:Cell Separation; D002875:Chromosomes / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000037:antagonists & inhibitors*; D005784:Gene Amplification; D005819:Genetic Markers / Q000235:genetics; D007621:Karyotyping; D051379:Mice; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D005819:Genetic Markers; D012334:RNA, Neoplasm; D004996:Ethidium
Poluchenie i geneticheskaia kharakteristika novoĭ linii kletok mielomy myshi spEBR-5, otselektirovannykh na ustoĭchivosti k bromistomu étidiiu i kharakterizuiushchikhsia mnozhestvennoĭ lekarstvennoĭ ustoĭchivost'iu.