title: Inverted repeat structure and homologous sequences in the LD1 amplicons of Leishmania spp.
abstract: In the parasitic trypanosomatids of the genus Leishmania, novel circular (CD) and linear (LD) multicopy genetic elements arise de novo either spontaneously or as a result of drug selection. We report that the LD1 minichromosomes of L. donovani, L. major and L. mexicana (ranging in size from 180 to 230 kb) have an inverted repeat structure and contain homologous sequences located at similar distances from the telomere; one half of the chromosome being the mirror image of the other. They must therefore have originated from a unique conserved source chromosome; the size polymorphism being generated by the point at which inversion occurs. The circular CD1 elements appear to be circularised segments of the LD1 elements. These observations lead to a unified concept of how minichromosomes LD1 and circular CD1 genetic elements emerge within the Leishmania and contribute to evolution of karyotype.
authors.affiliation: Instituut voor Moleculaire Biologie, Vrije Universiteit Brussel, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Navarro
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Muthui
authors.forename: G
authors.initials: G
authors.lastname: Ortiz
authors.forename: M
authors.initials: M
authors.lastname: Segovia
authors.forename: R
authors.initials: R
authors.lastname: Hamers
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002875:Chromosomes / Q000648:ultrastructure; D004270:DNA, Circular / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D005784:Gene Amplification; D007891:Leishmania / Q000145:classification / Q000235:genetics*; D007893:Leishmania donovani / Q000235:genetics; D018314:Leishmania infantum / Q000235:genetics; D018320:Leishmania major / Q000235:genetics; D007894:Leishmania mexicana / Q000235:genetics; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013045:Species Specificity
chemical_list: D004270:DNA, Circular; D016054:DNA, Protozoan