Restriction fragment length polymorphism of DQB and DRB class II genes of the ovine major histocompatibility complex.
The ovine major histocompatibility complex (MhcOvar) class II region was investigated by Southern blot hybridizations using ovine probes specific for the second exons of Ovar-DRB and Ovar-DQB genes. Multiple bands were revealed when genomic DNA was digested with each of five restriction enzymes (BamHI, EcoRI, HindIII, PvuII and TaqI), and successively hybridized with the two radiolabelled ovine probes. Restriction fragment length polymorphisms (RFLPs) were analysed in 89 sheep originating from six inbred families and the inheritance of the fragment patterns was determined. Forty-one fragments were recorded with the DQB probe; 32 were detected with the DRB probe. They constituted 9 DQB and 10 DRB allelic patterns. Twelve DQB-DRB haplotypes were resolved in this study.
Grain
F
F
Service de Zootechnie, Ecole Vétérinaire de Lyon, Marcy l'Etoile, France.
Nain
M C
MC
Labonne
M P
MP
Lantier
F
F
Lechopier
P
P
Gebuhrer
L
L
Asso
J
J
Maddox
J
J
Betuel
H
H
D000483:Alleles; D000818:Animals; D001345:Autoradiography / Q000662:veterinary; D015139:Blotting, Southern / Q000662:veterinary; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D015342:DNA Probes; D005260:Female; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep / Q000235:genetics*
D015342:DNA Probes; D000949:Histocompatibility Antigens Class II; D004247:DNA