CD11b/CD18 expression, adherence, and chemotaxis of granulocyte in adult respiratory distress syndrome.
The accumulation of granulocytes in the pulmonary microvasculature is generally thought a cardinal event in the pathology of adult respiratory distress syndrome (ARDS). However, the mechanism by which granulocytes are sequestered in the pulmonary vascular bed remains largely unknown. Because the CD11b/CD18 membrane receptors mediate various adhesion-dependent functions, their expression was investigated in granulocytes from patients during the course of ARDS development in relation to adherence and chemotaxis. CD11b expression of ARDS resting granulocytes was increased within 24 h of ARDS onset by a factor of two in comparison with control patients (p < 0.05) and remained significantly increased 72 to 120 h later. In contrast, the stimulated expression was significantly decreased only within 24 h of ARDS onset. Adherence was not modified within 8 h of the onset of ARDS, but was increased at Days 1, 3, and 5. The time course of granulocyte chemotaxis shows a decreased chemotaxis capacity during the first 3 d of ARDS, followed by normalization at Day 5. The dynamic changes observed in the various functions studied indicate a possible relationship between the modulation of the CD11b expression and a hyperadhesive state of granulocytes in ARDS. These sticky granulocytes may potentially contribute to the microvascular injury.
Laurent
T
T
Institut de Physiopathologie Clinique, CHUV, Lausanne, Switzerland.
Markert
M
M
Von Fliedner
V
V
Feihl
F
F
Schaller
M D
MD
Tagan
M C
MC
Chiolero
R
R
Perret
C
C
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D018845:CD11 Antigens; D018821:CD18 Antigens; D016022:Case-Control Studies; D002448:Cell Adhesion; D002634:Chemotaxis, Leukocyte / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D006098:Granulocytes / Q000276:immunology / Q000473:pathology*; D006801:Humans; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine; D011652:Pulmonary Circulation; D016028:Receptors, Leukocyte-Adhesion / Q000235:genetics / Q000276:immunology; D012128:Respiratory Distress Syndrome / Q000097:blood* / Q000209:etiology / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D013997:Time Factors
D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; D016028:Receptors, Leukocyte-Adhesion; D009240:N-Formylmethionine Leucyl-Phenylalanine