Cuticle-catalyzed coupling between polyamino acids and N-acetyldopamine.
N-[2-14C]Acetyldopamine (NADA) was incubated in vitro with a series of homopolyamino acids or proteins in the presence of cell-free cuticle from locusts. The oxidized NADA was bound to the materials in varying degrees. The results indicate that lysine and histidine sidechains in the cuticular proteins might be the most likely candidates as participants in the crosslinking process in agreement with the results obtained by NMR.
Wybrandt
G B
GB
Laboratory of Biochemical Chemistry A, August Krogh Institute, University of Copenhagen, Denmark.
Andersen
S O
SO
D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002250:Carbon Radioisotopes; D004298:Dopamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006110:Grasshoppers / Q000737:chemistry*; D006639:Histidine; D066298:In Vitro Techniques; D008239:Lysine; D011506:Proteins / Q000737:chemistry*
D000596:Amino Acids; D002250:Carbon Radioisotopes; D011506:Proteins; C006374:N-acetyldopamine; D006639:Histidine; D008239:Lysine; D004298:Dopamine