Identification and characterization of phosphoenolpyruvate:fructose phosphotransferase systems in three Streptomyces species.
Streptomyces lividans, S. coelicolor and S. griseofuscus were examined for the presence of the enzymes of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). All three species were shown to possess Enzyme I, HPr and fructose-specific Enzyme II (IIFru) activities. In S. lividans and S. coelicolor, all three PTS enzymes were fructose-inducible, but in S. griseofuscus the system was expressed constitutively. These organisms apparently lack the HPr(Ser) kinase and HPr(Ser-P) phosphatase that characterize low-GC Gram-positive bacteria.
Titgemeyer
F
F
Department of Biology, University of California at San Diego, La Jolla 92093-0116.
Walkenhorst
J
J
Reizer
J
J
Stuiver
M H
MH
Cui
X
X
Saier
M H
MH
D001412:Bacillus subtilis / Q000201:enzymology; D005632:Fructose / Q000378:metabolism; D005816:Genetic Complementation Test; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010766:Phosphorylation; D013045:Species Specificity; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D005632:Fructose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System