Characterization of pig-mink cell hybrids: assignment of the TK1 and UMPH2 genes to pig chromosome 12.
By fusion of thymidine kinase-deficient mink cells with pig leukocytes, a new type of cell hybrid was produced. It was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or chromosome fragmentation. With a set of subclones of two primary hybrid clones, the genes for thymidine kinase-1 (TK1) and uridine 5'-monophosphate hydrolase-2 (UMPH2) were assigned to pig Chromosome (Chr) 12. A cell line with a single pig Chr 8 on the background of mink chromosomes was established. This clone could serve as a source of DNA for building a chromosome-specific library of pig Chr 8. The data obtained suggest that pig-mink cell hybrids can be used for mapping of pig chromosomes.
Zhdanova
N S
NS
Developmental Genetics Laboratory, Russian Academy of Sciences, Siberian Department, Novosibirsk.
Astakhova
N M
NM
Kuznetsov
S B
SB
Schuler
L
L
Serov
O L
OL
D015720:5'-Nucleotidase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping / Q000379:methods; D003001:Cloning, Molecular; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D006822:Hybrid Cells; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000201:enzymology; D008297:Male; D008907:Mink; D016254:Mutagenesis, Insertional; D010710:Phosphates / Q000378:metabolism; D013552:Swine; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism
D007527:Isoenzymes; D010710:Phosphates; D013937:Thymidine Kinase; C092717:thymidine kinase 1; D015720:5'-Nucleotidase