An African swine fever virus gene with similarity to the T-lymphocyte surface antigen CD2 mediates hemadsorption.
An open reading frame, LMW8-DR, in the African swine fever virus (ASFV) genome possesses striking similarity to the lymphocyte membrane antigen CD2. All characterized CD2 domains, including the amino-terminal signal sequence, IgV, hinge, IgC2, stalk, transmembrane, and proline-rich carboxy cytoplasmic domains, are highly conserved in the ASFV gene. Critical residues for the binding of the lymphocyte function-associated antigen (LFA-3) and CD59 and for T-cell activation are also partially conserved. LMW8-DR is actively transcribed in ASFV-infected swine macrophages and Vero cells at late times in the infection cycle and Vero and COS cells transiently expressing the LMW8-DR open reading frame hemadsorbed swine red blood cells. The structural and functional similarities of LMW8-DR to CD2, a protein that is involved in cell-cell adhesion and immune response modulation, suggest a possible role in the pathogenesis of ASFV infection.
Borca
M V
MV
Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, New York 11944.
Kutish
G F
GF
Afonso
C L
CL
Irusta
P
P
Carrillo
C
C
Brun
A
A
Sussman
M
M
Rock
D L
DL
D000358:African Swine Fever Virus / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry*; D001483:Base Sequence; D018801:CD2 Antigens; D002478:Cells, Cultured; D017124:Conserved Sequence / Q000235:genetics; D005814:Genes, Viral / Q000502:physiology*; D006382:Hemadsorption / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011971:Receptors, Immunologic / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; C086096:LMW8-DR protein, African swine fever virus; D011971:Receptors, Immunologic; D014764:Viral Proteins