title: Temporal changes in intracellular free calcium levels in the developing neuroepithelium during neurulation in the chick.
abstract: Intracellular free calcium ion (Ca2+) levels of the developing chick neuroepithelium during neural tube closure (Hamburger and Hamilton stages 3-11 of embryonic development) were determined using the hydrophobic acetoxymethyl ester of the fluorescent dye fura-2 (fura-2/AM). Temporal changes in the free Ca2+ level in neuroepithelial cells are correlated with the degree of folding of the neuroepithelium. The concentration of intracellular Ca2+ in the neuroepithelium reaches its highest level when apposing neural folds are actively making contact.
authors.affiliation: Department of Biology, Rutgers University, Camden, NJ 08102.
authors.forename: J V
authors.initials: JV
authors.lastname: Martin
authors.forename: R G
authors.initials: RG
authors.lastname: Nagele
authors.forename: H Y
authors.initials: HY
authors.lastname: Lee
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002490:Central Nervous System / Q000196:embryology* / Q000378:metabolism*; D002642:Chick Embryo / Q000378:metabolism*; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D013997:Time Factors
chemical_list: D002118:Calcium; D009638:Norepinephrine