Use of antineutrophil cytoplasmic autoantibodies in diagnosing vasculitis in a Chinese patient population.
Antineutrophil cytoplasmic autoantibodies (ANCA) have been used as markers of systemic vasculitides, including microscopic polyarteritis (MPA) and Wegener's granulomatosis. The diagnostic potential of ANCA assays together with antibodies against the neutrophil enzymes myeloperoxidase (MPO) and proteinase 3 for detecting a systemic vasculitis was tested in a Chinese patient population. 672 sera were received for ANCA assay, and ANCA detected by indirect immunofluorescence was positive in 73 sera from 42 patients. Of the 42 patients, 3 had cytoplasmic ANCA, while 39 had a perinuclear pattern. There was no patient with Wegener's granulomatosis. Two cytoplasmic ANCA positive patients suffered from ulcerative colitis. Another cytoplasmic ANCA positive patient was a carrier of human immunodeficiency virus. Of the 39 perinuclear ANCA positive patients, 10 had MPA. Eight of them were tested for anti-MPO antibody, and all were positive. Other immune disorders that were perinuclear ANCA positive included: 13 patients with systemic lupus erythematosus, 3 with mixed connective tissue disease, 1 with Goodpasture's syndrome, 2 with inflammatory bowel disease, and 2 patients with IgA nephropathy. Anti-MPO antibody was not specific for MPA, and 7 out of the 13 patients with systemic lupus erythematosus were anti-MPO antibody positive. Our study suggests that ANCA and anti-MPO antibody are not specific for MPA in a Chinese population. They would alert the clinician of the possibility of vasculitis, but a clinicopathological correlation is essential in making the diagnosis.
Li
P K
PK
Department of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin.
Leung
J C
JC
Lai
F M
FM
Wang
A
A
Lui
S F
SF
Leung
C B
CB
Lai
K N
KN
D000293:Adolescent; D000328:Adult; D000368:Aged; D019867:Anti-Glomerular Basement Membrane Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D002681:China; D003093:Colitis, Ulcerative / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D005922:Glomerulonephritis, IGA / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D014890:Granulomatosis with Polyangiitis / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008947:Mixed Connective Tissue Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D053146:Myeloblastin; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism; D010488:Polyarteritis Nodosa / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D011237:Predictive Value of Tests; D012697:Serine Endopeptidases / Q000378:metabolism; D014657:Vasculitis / Q000097:blood / Q000175:diagnosis* / Q000208:ethnology / Q000276:immunology
D001323:Autoantibodies; D015415:Biomarkers; D009195:Peroxidase; D012697:Serine Endopeptidases; D053146:Myeloblastin