Postlabeling: a sensitive method for studying DNA adducts and their role in carcinogenesis.
The covalent binding of xenobiotics to DNA is an important trigger of the multistage process that leads to carcinogenesis. 32P-postlabeling represents a highly sensitive method for biomonitoring exposure to genotoxic agents and for cancer risk assessment; it is capable of detecting less than one DNA adduct per human genome. Recent improvements to the technique have shown that the resistance of adducted DNA to enzyme digestion may lead to an overestimation of the number of different adducts present in a sample.
Keith
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Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, Strasbourg, France.
Dirheimer
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D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D018736:DNA Adducts / Q000032:analysis*; D015894:Genome, Human; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D010761:Phosphorus Radioisotopes; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology; D011865:Radioisotope Dilution Technique; D012680:Sensitivity and Specificity; D015262:Xenobiotics
D018736:DNA Adducts; D010761:Phosphorus Radioisotopes; D015262:Xenobiotics