title: Comparison of virulence between Seoul virus strain SR-11 and Hantaan virus strain 76-118 of hantaviruses in newborn mice.
abstract: Virulence of hantavirus strain of SR-11 Seoul virus and Hantaan 76-118 (HTN) of Hantaan virus were compared. Infections of both strains were lethal in newborn mice. However, inoculum required to cause lethal infection was about 4,000 times higher for strain HTN (1.65 x 10(3) PFU/mouse/LD50) than for strain SR-11 (0.36 PFU). Thus, both strains were considered pathogenic to newborn mice but they possessed different levels of virulence. The assay system used for these strains in newborn mice proved to be useful in the study of hantavirus virulence. Growth curves of the two strains in CV-7 cell cultures were compared. Strain SR-11 was shown to have higher activity of virus replication and virus release into the culture fluids than strain HTN. The possibility of a relationship between replication activity and high levels of virulence in mice was suggested.
authors.affiliation: Institute of Immunological Science, Hokkaido University, Japan.
authors.forename: Y C
authors.initials: YC
authors.lastname: Yoo
authors.forename: K
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: R
authors.initials: R
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Tamura
authors.forename: I
authors.initials: I
authors.lastname: Azuma
authors.forename: J
authors.initials: J
authors.lastname: Arikawa
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D018063:Hantaan virus / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D006476:Orthohantavirus / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology / Q000401:mortality*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D016019:Survival Analysis; D014018:Tissue Distribution; D010948:Viral Plaque Assay; D014774:Virulence
chemical_list: D000914:Antibodies, Viral