Expression of biologically active receptors for natriuretic peptides in the human uterus during pregnancy.
Human amnion cells secrete a large amount of brain natriuretic peptide (BNP). In the present study, to elucidate the possible roles of amniotic BNP during the course of human pregnancy, the tissue distributions of biologically active receptors of natriuretic peptides, atrial natriuretic peptide (ANP)-A receptor and ANP-B receptor in the human uterus during pregnancy were investigated by Northern blot analysis and an in vitro guanosine 3',5'-cyclic phosphate (cGMP) generation assay, the second messenger of natriuretic peptides, using crude membrane preparations. Both ANP-A and ANP-B receptor mRNAs were detected in the tissues of decidua vera, chorion laeve, myometrium and placenta. The in vitro cGMP generation assay revealed that the expression of ANP-A receptor was more prominent than that of ANP-B receptor in these tissues. On the other hand, in amniotic tissue only ANP-B receptor mRNA was weakly expressed but ANP-A receptor mRNA was not detected.
Itoh
H
H
Department of Gynecology and Obstetrics, Kyoto University Faculty of Medicine, Japan.
Sagawa
N
N
Hasegawa
M
M
Nanno
H
H
Kobayashi
F
F
Ihara
Y
Y
Mori
T
T
Komatsu
Y
Y
Suga
S
S
Yoshimasa
T
T
D009320:Atrial Natriuretic Factor / Q000378:metabolism* / Q000494:pharmacology; D001483:Base Sequence; D015152:Blotting, Northern; D002462:Cell Membrane / Q000201:enzymology; D017931:DNA Primers; D015342:DNA Probes; D005321:Extraembryonic Membranes / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006162:Guanylate Cyclase / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009215:Myometrium / Q000378:metabolism; D020097:Natriuretic Peptide, Brain; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D011506:Proteins / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000096:biosynthesis*; D014599:Uterus / Q000378:metabolism*
D017931:DNA Primers; D015342:DNA Probes; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger; D020097:Natriuretic Peptide, Brain; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor