title: Detection of epidermodysplasia verruciformis-like human papillomavirus types in malignant and premalignant skin lesions of renal transplant recipients.
abstract: To evaluate the putative role of human papillomaviruses (HPV) in the development of skin cancer in renal transplant recipients, a series of skin biopsies from premalignant and malignant skin lesions was analysed using the polymerase chain reaction. Four different consensus primer pairs were used. HPV DNA was detected in five of 24 cases of squamous cell carcinoma, in one of three cases of Bowen's disease, in none of four basal cell carcinomas, in two of seven cases of actinic keratosis and in one of five cases of keratoacanthoma. Typing by direct sequencing of the amplified HPV DNA was possible in seven of nine cases, and revealed epidermodysplasia verruciformis (EV)-associated HPV types, or HPV types related to EV-associated types. Hence, HPV DNA could be detected in a significant proportion of (pre)malignant skin tumours in renal transplant recipients. The finding that some of the detected HPV types were as yet uncharacterized EV-related types, suggests that HPV DNA could be present in a higher percentage of lesions, and might be detected with refinement of the techniques.
authors.affiliation: Department of Dermatology, University Hospital Leiden, The Netherlands.
authors.forename: L M
authors.initials: LM
authors.lastname: Tieben
authors.forename: R J
authors.initials: RJ
authors.lastname: Berkhout
authors.forename: H L
authors.initials: HL
authors.lastname: Smits
authors.forename: J N
authors.initials: JN
authors.lastname: Bouwes Bavinck
authors.forename: B J
authors.initials: BJ
authors.lastname: Vermeer
authors.forename: J A
authors.initials: JA
authors.lastname: Bruijn
authors.forename: F J
authors.initials: FJ
authors.lastname: Van der Woude
authors.forename: J
authors.initials: J
authors.lastname: Ter Schegget
mesh_terms: D001483:Base Sequence; D001913:Bowen's Disease / Q000821:virology; D002280:Basal Cell Carcinoma / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000821:virology; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D004819:Epidermodysplasia Verruciformis / Q000821:virology*; D006801:Humans; D007636:Keratoacanthoma / Q000821:virology; D016030:Kidney Transplantation; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D010787:Photosensitivity Disorders / Q000821:virology; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000821:virology*; D012878:Skin Neoplasms / Q000821:virology*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral