title: Coenzyme A glutathione disulfide. A potent vasoconstrictor derived from the adrenal gland.
abstract: The adrenal gland is involved in the regulation of vascular tone by secretion of vasoactive agents such as catecholamines, neuropeptide Y, or endogenous ouabain. A further potent vasoconstrictor is isolated from bovine adrenal glands and is identified by chromatography, mass spectrometry, UV spectroscopy, and enzymatic cleavage as coenzyme A glutathione disulfide (CoASSG). CoASSG is found in chromaffin granules of adrenal glands and is released from adrenal medulla slices by carbachol. At a concentration of 10(-12) mol/L CoASSG increases renal vascular resistance. Intra-aortic injection of 5 x 10(-10) mol CoASSG increases blood pressure in the intact animal. Besides its vasopressor properties, this substance potentiates the effects of angiotensin II on vascular tone. It is concluded that CoASSG could play a role in blood pressure regulation not only by direct effects but also by modulation of the action of angiotensin II.
authors.affiliation: Medizinische Poliklinik, Universität Münster, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schlüter
authors.forename: M
authors.initials: M
authors.lastname: Meissner
authors.forename: M
authors.initials: M
authors.lastname: van der Giet
authors.forename: M
authors.initials: M
authors.lastname: Tepel
authors.forename: J
authors.initials: J
authors.lastname: Bachmann
authors.forename: I
authors.initials: I
authors.lastname: Gross
authors.forename: E
authors.initials: E
authors.lastname: Nordhoff
authors.forename: M
authors.initials: M
authors.lastname: Karas
authors.forename: C
authors.initials: C
authors.lastname: Spieker
authors.forename: H
authors.initials: H
authors.lastname: Witzel
mesh_terms: D000311:Adrenal Glands / Q000737:chemistry*; D000313:Adrenal Medulla / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002118:Calcium / Q000032:analysis; D002217:Carbachol / Q000494:pharmacology; D002417:Cattle; D002837:Chromaffin Granules / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003065:Coenzyme A / Q000302:isolation & purification* / Q000494:pharmacology; D006439:Hemodynamics; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects; D013058:Mass Spectrometry; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000166:cytology; D010477:Perfusion; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013268:Stimulation, Chemical; D014655:Vascular Resistance / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D014662:Vasoconstrictor Agents; C019305:coenzyme A-glutathione mixed disulfide; D002217:Carbachol; D003065:Coenzyme A; D002118:Calcium