Photorepair of nonadjacent pyrimidine dimers by DNA photolyase.
Photolyases reverse the harmful effects of UV light on cells by converting pyrimidine dimers (Pyr[]Pyr) into two pyrimidine monomers by utilizing near-UV and visible light. Previous work has shown that photolyase repairs T[c,s]T and T[t,s]T in DNA as well as U[]U in RNA, all of which are formed by joining the two adjacent pyrimidines in a light-dependent reaction. In this report, we show that Pyr[]Pyr formed in nonadjacent pyrimidines are also substrates for DNA photolyase.
Kim
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Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27514-7260.
Sancar
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D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D011740:Pyrimidine Dimers / Q000378:metabolism*; D013379:Substrate Specificity; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D004255:Deoxyribodipyrimidine Photo-Lyase