Evidence for the expression of dynorphin gene in the prolactin-immunoreactive neurons of the rat lateral hypothalamus.
Dynorphin B (DYN B) immunoreactivity was recently reported in a population of prolactin (PRL)-immunoreactive neurons of the rat lateral hypothalamus (LH). By coupling immunocytochemistry and in situ hybridization using two synthetic oligonucleotide probes complementary to DYN mRNA, a hybridization signal was observed over the neurons of the LH exhibiting both DYN- and PRL-like immunoreactivities. Our results clearly demonstrate that these neurons contain the mRNA encoding preproDYN and are able to synthesize authentic DYN B in colocalization with a peptide related to PRL.
Griffond
B
B
CNRS UA 561, Laboratoire d'Histologie, Embryologie, Cytogénétique, Faculté de Médecine, Besançon, France.
Colard
C
C
Deray
A
A
Fellmann
D
D
Bugnon
C
C
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D004399:Dynorphins / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics; D004723:Endorphins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000502:physiology*; D006651:Histocytochemistry; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D015345:Oligonucleotide Probes; D011388:Prolactin / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats
D004723:Endorphins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004399:Dynorphins; C036200:rimorphin; D011388:Prolactin