Cloning and gene map assignment of the Xiphophorus DNA ligase 1 gene.
Fishes represent the stem vertebrate condition and have maintained several gene arrangements common to mammalian genomes throughout the 450 Myr of divergence from a common ancestor. One such syntenic arrangement includes the GPI-PEPD enzyme association on Xiphophorus linkage group IV and human chromosome 19. Previously we assigned the Xiphophorus homologue of the human ERCC2 gene to linkage group U5 in tight association with the CKM locus. CKM is also tightly linked to the ERCC2 locus on human chromosome 19, leading to speculation that human chromosome 19 may have arisen by fusion of two ancestral linkage groups which have been maintained in fishes. To investigate this hypothesis further, we isolated and sequenced Xiphophorus fish genomic regions exhibiting considerable sequence similarity to the human DNA ligase 1 amino acid sequence. Comparison of the fish DNA ligase sequence with those of other species suggests several modes of amino acid conservation in this gene. A 2.2-kb restriction fragment containing part of an X. maculatus DNA ligase 1 exon was used in backcross hybrid mapping with 12 enzyme or RFLP loci. Significant linkage was observed between the nucleoside phosphorylase (NP2) and the DNA ligase (LIG1) loci on Xiphophorus linkage group VI. This assignment suggests that the association of four DNA repair-related genes on human chromosome 19 may be the result of chance chromosomal rearrangements.
Walter
R B
RB
Department of Biology, Southwest Texas State University, San Marcos 78666.
Rolig
R L
RL
Kozak
K A
KA
McEntire
B
B
Morizot
D C
DC
Nairn
R S
RS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D003532:Cyprinodontiformes / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis; D005091:Exons; D008040:Genetic Linkage; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C000607892:LIG1 protein, human; D004247:DNA; D011088:DNA Ligases; D000072481:DNA Ligase ATP