A consensus Alu repeat probe for physical mapping.
Physical mapping of the human genome involves a variety of complex hybridization-based procedures, some of which rely upon the ability to separate human clones derived from human-rodent hybrid cell lines from those that contain background rodent-derived DNA sequences. The ability to block the repetitive element (Alu repeat) portion of inter-Alu PCR products derived from a variety of complex sources is also crucial for the isolation of unique DNA sequences. Here we report the construction and characterization of a new consensus Alu repeat probe (pPD39) designed for these purposes.
Batzer
M A
MA
Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California 94551.
Alegria-Hartman
M
M
Deininger
P L
PL
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D016384:Consensus Sequence; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics*; D005821:Genetic Techniques; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers; D015342:DNA Probes; D007202:Indicators and Reagents