title: v-erbA stimulates quail myoblast differentiation in a T3 independent, cell-specific manner.
abstract: The v-erbA oncoprotein represents a mutated version of a thyroid hormone receptor, responsible for the induction of a differentiation arrest in chicken erythroid cells. We have studied the influence of v-erbA on proliferation and differentiation of avian myoblasts. Secondary quail myoblast cultures were infected either with an avian retrovirus carrying the v-erbA oncogene in association with the neomycin resistance gene, or with a control deleted v-erbA/neoR alpha retrovirus. We report here that v-erbA expression led to an increase in myoblast proliferation and to a surprising stimulation of quail myoblast terminal differentiation. In addition, these effects occurred in the presence or absence of T3, and v-erbA did not suppress T3 influence on myoblasts. Transient transfection assays demonstrated that, in contrast to its action in HeLa cells, v-erbA was unable to repress the transcriptional activation of a TRE-CAT reporter gene by liganded c-erbA alpha receptors in quail myoblasts. We also observed that the AP-1/c-erbA/v-erbA interactions are not functional in quail myoblasts. These data suggest that, in these cells, v-erbA action does not interfere with T3 induced mechanisms. They also demonstrate a cell specificity for the v-erbA pathway. Lastly, expression of c-erbA/v-erbA chimeric proteins and of the S61G v-erbA mutant indicates that the DNA binding domain of v-erbA, and more specifically serine 61, is directly involved in the enhancement of myoblast differentiation by the oncoprotein.
authors.affiliation: INRA, Unité d'Endocrinologie Cellulaire, Laboratoire de Différenciation Cellulaire et Croissance, Montpellier, France.
authors.forename: I
authors.initials: I
authors.lastname: Cassar-Malek
authors.forename: S
authors.initials: S
authors.lastname: Marchal
authors.forename: M
authors.initials: M
authors.lastname: Altabef
authors.forename: C
authors.initials: C
authors.lastname: Wrutniak
authors.forename: J
authors.initials: J
authors.lastname: Samarut
authors.forename: G
authors.initials: G
authors.lastname: Cabello
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003370:Coturnix; D004247:DNA / Q000378:metabolism; D006801:Humans; D009132:Muscles / Q000196:embryology*; D018774:Oncogene Proteins v-erbA; D016755:Proto-Oncogene Proteins c-jun / Q000502:physiology; D015682:Retroviridae Proteins, Oncogenic / Q000502:physiology*; D015533:Transcriptional Activation; D014284:Triiodothyronine / Q000502:physiology*; D016335:Zinc Fingers
chemical_list: D018774:Oncogene Proteins v-erbA; D016755:Proto-Oncogene Proteins c-jun; D015682:Retroviridae Proteins, Oncogenic; D014284:Triiodothyronine; D004247:DNA