Detection of gastrin mRNA in paraffin-embedded samples of normal antral mucosae using polymerase chain reaction technique.
Gastrin, a peptide hormone produced by the G cells of the gastric antrum, plays a major role in the regulation of digestive mucosal growth. Although some light has been shed on the peptidic aspects of this hormone's mode of action and the co-regulatory activity in which it is involved along with the other gastrointestinal hormones, little is known at present about the modes of expression of its mRNA at the tissue level. A few attempts have been made so far to detect the transcript, mostly using molecular hybridization techniques. Here it was proposed to detect gastrin mRNA using a RT-PCR technique on a series of paraffin-embedded samples of normal human antrum processed with various fixatives commonly used in histology. The transcript was detectable in all the 7-microns sections of the samples treated with either formalin or Carnoy's solution, whereas Bouin's solution, which is also used as a fixative in histology, was found to have inhibitory effects on the method described here.
Biagini
P
P
Regional Center for Blood Transfusion, Paoli-Calmettes Institute, Marseille, France.
Monges
G
G
Cantaloube
J F
JF
Parriaux
D
D
Hassoun
J
J
Chicheportiche
C
C
D000085:Acetates; D019342:Acetic Acid; D001483:Base Sequence; D015139:Blotting, Southern; D002725:Chloroform; D000431:Ethanol; D005404:Fixatives; D005557:Formaldehyde; D005753:Gastric Mucosa / Q000737:chemistry*; D005755:Gastrins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D010851:Picrates; D016133:Polymerase Chain Reaction; D011706:Pyloric Antrum / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics
D000085:Acetates; C033474:Carnoy's solution; D005404:Fixatives; D005755:Gastrins; D010851:Picrates; D012333:RNA, Messenger; D005557:Formaldehyde; C026239:Bouin's solution; D000431:Ethanol; D002725:Chloroform; D019342:Acetic Acid