title: nm23-H1 protein immunoreactivity in cancers of the gallbladder, extrahepatic bile ducts and ampulla of Vater.
abstract: Immunohistochemical expression of the nm23-H1 protein was investigated in carcinoma of the gallbladder (n = 13), common bile duct (n = 5) and ampulla of Vater (n = 7) using the monoclonal antibody, NM 301 (Molecular Oncology Inc.). This was compared with cases of chronic cholecystitis (n = 11) and preneoplastic lesions of the gallbladder (n = 4) and ampulla (n = 3). Absent or weak nm23-H1 protein immunoreactivity was found in most (67%) of the poorly differentiated adenocarcinomas of the gallbladder and in half of the cases of moderately differentiated adenocarcinomas. The difference in nm23-H1 protein immunostaining between gallbladder carcinoma and chronic cholecystitis was statistically significant (p = 0.0022; X2 test). Significant correlation was found between prolonged patient survival periods and nm23-H1 immunoreactivity in gallbladder carcinomas (R = 0.687, p = 0.01, linear regression analysis; r = 0.652, p = 0.016, Spearman's rank test). Most of the gallbladder dysplasias and adenomas had no reduction in nm23-H1 protein immunoreactivity. All common bile duct carcinomas, most (67%) ampullary carcinomas in situ and some (43%) ampullary carcinomas had moderate to strong nm23-H1 immunostaining. In summary, low nm23-H1 protein immunoreactivity was found more frequently in gallbladder carcinomas than in chronic cholecystitis and was associated with reduced patient survival. The differences in nm23-H1 protein immunoreactivity in common bile duct and ampullary carcinomas suggest that these tumors have a different molecular origin to gallbladder cancers.
authors.affiliation: Department of Pathology, University of Melbourne, Victoria.
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
authors.forename: A
authors.initials: A
authors.lastname: Pirdas-Zivcic
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry; D000236:Adenoma / Q000737:chemistry; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014670:Ampulla of Vater / Q000473:pathology; D001650:Bile Duct Neoplasms / Q000737:chemistry*; D017734:Bile Ducts, Extrahepatic / Q000473:pathology; D002278:Carcinoma in Situ / Q000737:chemistry; D002764:Cholecystitis / Q000378:metabolism; D003138:Common Bile Duct Neoplasms / Q000737:chemistry*; D005260:Female; D005706:Gallbladder Neoplasms / Q000737:chemistry* / Q000401:mortality; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins / Q000032:analysis*; D009701:Nucleoside-Diphosphate Kinase; D016019:Survival Analysis; D014157:Transcription Factors / Q000032:analysis*
chemical_list: D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins