title: Non-homologous recombination within the major histocompatibility complex creates a transcribed hybrid sequence.
abstract: The P5-1 cDNA clone maps to the human MHC class I region (Vernet et al. 1993a). In this paper, we show that the P5-1 cDNA represents a chimeric transcript in which the first exon of an MHC class I gene has been spliced to an unrelated sequence. The corresponding gene P5-1 is composed of the 5' sequence of an MHC class I gene including the promoter region, the first exon, and the half of the first intron fused to an unrelated intron, followed by a large exon. Furthermore, the non-class I part of P5-1 is present within the MHC class I region in multiple copies, defining the P5 family. Another member of the P5 family is fused to a class I gene, although by a type of rearrangement different from P5-1. These two fusion events between members of HLA class I and P5 families reflect the existence of a duplication unit including two class I genes and a P5 sequence. These data shed light on the MHC class I evolution and on the creation and evolution of new genes.
authors.affiliation: CNRS UPR 8291, CHU Purpan, Toulouse, France.
authors.forename: P
authors.initials: P
authors.lastname: Avoustin
authors.forename: M T
authors.initials: MT
authors.lastname: Ribouchon
authors.forename: C
authors.initials: C
authors.lastname: Vernet
authors.forename: B
authors.initials: B
authors.lastname: N'Guyen
authors.forename: B
authors.initials: B
authors.lastname: Crouau-Roy
authors.forename: P
authors.initials: P
authors.lastname: Pontarotti
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005091:Exons; D015870:Gene Expression; D005805:Genes, MHC Class I / Q000235:genetics*; D006801:Humans; D007438:Introns; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D018076:DNA, Complementary; C080521:HCP5 long noncoding RNA, human; D011506:Proteins; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D011993:Recombinant Fusion Proteins