The role of HSV-induced Fc- and C3b (i)-receptors in bacterial adherence.
Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in bacterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fluorescent quantitation of FITC-labelled bacteria, was developed. Opsonisation of Staphylococcus epidermidis with human serum, resulted in a marked increase in adherence to HSV-infected cells and revealed a role for C3b(i)R- and FcR-mediated adhesion. However, the enhanced adherence never exceeded the level of attachment to non-infected cells. Increased adherence of other pathogenic bacteria, including Escherichia coli, Streptococcus pneumoniae, Haemophilus influenzae and Pseudomonas aeruginosa was not observed, indicating that the HSV-receptors play a minor role in secondary infections. Bacterial adhesion factors such as the fimbriae of E. coli played a more dominant role in the adherence of bacteria to HSV-infected cells.
De Graaf-Miltenburg
L A
LA
Eijkman-Winkler Laboratory of Medical Microbiology, Utrecht University, The Netherlands.
Van Vliet
K E
KE
Ten Hagen
T L
TL
Verhoef
J
J
Van Strijp
J A
JA
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D001419:Bacteria / Q000276:immunology / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000276:immunology*; D001667:Binding, Competitive; D002460:Cell Line; D004926:Escherichia coli / Q000276:immunology / Q000378:metabolism; D010861:Fimbriae, Bacterial; D005434:Flow Cytometry; D006193:Haemophilus influenzae / Q000276:immunology / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D009895:Opsonin Proteins / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology / Q000378:metabolism; D017463:Receptors, Complement 3b / Q000502:physiology*; D011961:Receptors, Fc / Q000502:physiology*; D013212:Staphylococcus epidermidis / Q000276:immunology / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D007074:Immunoglobulin G; D009895:Opsonin Proteins; D017463:Receptors, Complement 3b; D011961:Receptors, Fc