title: Light and electron microscopical immunohistochemical localization of the small proteoglycan core proteins decorin and biglycan in human knee joint cartilage.
abstract: The distribution of decorin and biglycan was investigated at the light and electron microscopical level in adult human articular cartilage. In general, the amount of decorin and biglycan was found to decrease with the depth of the layer of the cartilage. Decorin was found in the interterritorial matrix where most of the collagen is located. This fits in well with the assumption that decorin may modulate collagen metabolism. Biglycan was found next to the chondrocytes in the pericellular matrix and is assumed to be responsible for cellular activities. At the ultrastructural level, decorin was localized in the interterritorial matrix and in vesicles in chondrocytes. Biglycan was found, usually though not exclusively in the pericellular matrix. Both small proteoglycans were detected close to and on the collagen fibres and also associated with the more globular structures of the matrix between the fibrils. A double-staining approach revealed that the two molecules could be located along the same collagen fibril. However, staining for biglycan and decorin was not observed simultaneously within the same region of the fibre.
authors.affiliation: Zentrum Anatomie, Abteilung Histologie, Göttingen, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Miosge
authors.forename: K
authors.initials: K
authors.lastname: Flachsbart
authors.forename: W
authors.initials: W
authors.lastname: Goetz
authors.forename: W
authors.initials: W
authors.lastname: Schultz
authors.forename: H
authors.initials: H
authors.lastname: Kresse
authors.forename: R
authors.initials: R
authors.lastname: Herken
mesh_terms: D000328:Adult; D058578:Biglycan; D002358:Cartilage, Articular / Q000737:chemistry* / Q000648:ultrastructure; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007719:Knee Joint / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D011509:Proteoglycans / Q000032:analysis*
chemical_list: C546884:BGN protein, human; D058578:Biglycan; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans