Characterization of the cit-1 gene from Neurospora crassa encoding the mitochondrial form of citrate synthase.
We have isolated the cDNA and corresponding genomic DNA encoding citrate synthase in Neurospora crassa. Analysis of the protein coding region of this gene, named cit-1, indicates that it specifies the mitochondrial form of citrate synthase. The predicted protein has 469 amino acids and a molecular mass of 52,002 Da. The gene is interrupted by four introns. Hybridization experiments show that a cit-1 probe binds to two different fragments of genomic DNA, which are located on different chromosomes. Neurospora crassa may have two isoforms of citrate synthase, one in the mitochondria and the other in microbodies.
Ferea
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Department of Biology, Sinsheimer Laboratories, University of California, Santa Cruz 95064.
Contreras
E T
ET
Oung
T
T
Bowman
E J
EJ
Bowman
B J
BJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002950:Citrate (si)-Synthase / Q000737:chemistry / Q000235:genetics*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D007438:Introns; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D002950:Citrate (si)-Synthase