Regulated expression of Brachyury(T), Nkx1.1 and Pax genes in embryoid bodies.
Embryonic stem cells (ES) can be exploited to analyze in vitro mechanisms of cellular differentiation. We have utilized ES-derived embryoid body formation in an attempt to study cell types resulting from in vitro differentiation. To this end, a variety of molecular markers, preferably those which have been associated with regulatory events during mouse embryogenesis, was employed. Specifically, Brachyury (T), Pax-3 and Pax-6 genes as well as Nkx-1.1 were used. We could demonstrate that the expression of these genes in vitro was regulated by growth factors such as activin A or bFGF. Implications of these findings and the possible applications for identifying new genes are discussed.
Yamada
G
G
Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Göttingen, Germany.
Kioussi
C
C
Schubert
F R
FR
Eto
Y
Y
Chowdhury
K
K
Pituello
F
F
Gruss
P
P
D000818:Animals; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D004625:Embryo, Nonmammalian; D005314:Embryonic and Fetal Development; D005136:Eye Proteins; D005326:Fetal Proteins / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects; D005801:Genes, Homeobox; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D018398:Homeodomain Proteins; D017404:In Situ Hybridization, Fluorescence; D008648:Mesoderm / Q000187:drug effects / Q000502:physiology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000502:physiology; D000072041:PAX3 Transcription Factor; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D014981:Xenopus; D029867:Xenopus Proteins; D000098908:Brachyury Protein
D004268:DNA-Binding Proteins; D005136:Eye Proteins; D005326:Fetal Proteins; D005904:Glial Fibrillary Acidic Protein; D018398:Homeodomain Proteins; D009414:Nerve Growth Factors; D000072041:PAX3 Transcription Factor; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D029867:Xenopus Proteins; D000098908:Brachyury Protein; C489411:Pax3 protein, Xenopus; C000605732:Pax6 protein, mouse; C068686:Pax3 protein, mouse