[Cloning and identification of the gene controlling nitrogen metabolism in the photosynthetic purple bacteria Rhodobacter sphaeroides].
A recombinant plasmid has been selected from the genomic library of Rhodobacter sphaeroides that restores the properties of the wild type strain in the mutant Drn121. The latter possesses the derepressed synthesis of nitrogenase when grown in the light, inability of nitrogen fixation in the dark and growth on potassium nitrate as a single source of nitrogen, disruption of ammonium ions and methylamine transportation, decreased activity of glutamine synthetase. The gene complementing the drn121 mutation is localized within the EcoRI-HindIII fragment of Rhodobacter sphaeroides chromosome 2.25 kb in size. Analysis of the fragment nucleotide sequence has revealed the fragments with a high level of homology to regulatory genes ntrB (the 3'-end) and ntrC of Rhodobacter capsulatus. The plasmid pRCN102, containing the nifR3-ntrB-ntrC operon of Rhodobacter capsulatus, is able to complement the drn121 mutation while its derivatives having inactivated ntrN or ntrC genes are not. Hence, in Rhodobacter sphaeroides mutant Drn121 the mutation is localized in ntrC gene the product of which is involved not only in nitrogen fixation but also in nitrogen metabolism on the whole.
Glazer
V M
VM
Tevzadze
G G
GG
Babykin
M M
MM
Smirnova
V A
VA
Zinchenko
V V
VV
Shestakov
S V
SV
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism*; D009586:Nitrogen Fixation / Q000235:genetics; D009876:Operon; D010788:Photosynthesis; D010957:Plasmids; D012242:Rhodobacter sphaeroides / Q000235:genetics* / Q000378:metabolism
D004269:DNA, Bacterial; D009584:Nitrogen
Klonirovanie i identifikatsiia gena, kontroliruiushchego azotistyĭ metabolizm u fotosinteziruiushcheĭ purpurnoĭ bakterii Rhodobacter sphaeroides.