Comparative RFLP mapping of an allotetraploid wild rice species (Oryza latifolia) and cultivated rice (O. sativa).
The purpose of this study was to construct a comparative RFLP map of an allotetraploid wild rice species, Oryza latifolia, and to study the relationship between the CCDD genome of O. latifolia and the AA genome of O. sativa. A set of RFLP markers, which had been previously mapped to the AA genome of cultivated rice, were used to construct the comparative map. Fifty-eight F2 progeny, which were derived from a single F1 plant, were used for segregation analysis. The comparative RFLP map contains 149 DNA markers, including 145 genomic DNA markers from cultivated rice, 3 cDNA markers from oat, and one known gene (waxy, from maize). Segregation patterns reflected the allotetraploid ancestry of O. latifolia, and the CC and DD genomes were readily distinguished by most probes tested. There is a high degree of conservation between the CCDD genome of O. latifolia and the AA genome of O. sativa based on our data, but some inversions and translocations were noted.
Huang
H
H
Botany Department, University of Georgia, Athens 30602.
Kochert
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G
D005075:Biological Evolution; D004247:DNA / Q000302:isolation & purification; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D017343:Genes, Plant; D016678:Genome; D012275:Oryza / Q000235:genetics*; D011003:Ploidies; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013045:Species Specificity
D015342:DNA Probes; D004247:DNA; D004262:DNA Restriction Enzymes