Comparison of protein metabolism and glucose uptake in turkey (Melegaris gallopavo) satellite cells and embryonic myoblasts in vitro.
Protein synthesis, protein degradation, and glucose uptake were compared in clonal-derived turkey myogenic satellite cells (clone D5-SC) and embryonic myoblasts (EM) and between satellite cell cultures from Nicholas (DN) and Merriam's (WM) turkeys. Protein synthesis rates were higher and degradation rates were lower in myotube cultures of D5-SC compared with EM (P < or = 0.05). Protein synthesis and degradation rates did not differ between cultures of DN and WM (P > or = 0.05). Glucose transport rates were significantly higher in EM than D5-SC cultures and did not differ between DN and WM cultures. Insulin-like growth factors and insulin stimulated protein synthesis, decreased protein degradation, and increased glucose uptake in all cell lines.
McFarland
D C
DC
Department of Animal and Range Sciences, South Dakota State University, Brookings 57007-0392.
Pesall
J E
JE
Gilkerson
K K
KK
Ferrin
N H
NH
Ye
W V
WV
Swenning
T A
TA
D000818:Animals; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D014422:Turkeys / Q000196:embryology / Q000378:metabolism*
D007328:Insulin; D011506:Proteins; D007334:Insulin-Like Growth Factor I; D005947:Glucose