title: Isolation of intergeneric hybrids between Bacillus subtilis and Zymomonas mobilis and the production of thermostable amylase by hybrids.
abstract: Stable hybrids were obtained by protoplast fusion between Bacillus subtilis and Zymomonas mobilis. All the hybrids were able to hydrolyse starch and possessed ampicillin- and tetracycline-resistant phenotypes. Two of the hybrids, BZ-1 and BZ-2, were hyperproducers of alpha-amylase. The enzyme produced by these hybrids exhibited increased thermostability. The results show that stable intergeneric gene transfer can be achieved through poly(ethylene glycol)-mediated protoplast fusion between two industrially important genera of bacteria.
authors.affiliation: NCIM, Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
authors.forename: D
authors.initials: D
authors.lastname: Gokhale
authors.forename: D
authors.initials: D
authors.lastname: Deobagkar
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D004795:Enzyme Stability; D006358:Hot Temperature; D006824:Hybridization, Genetic; D011523:Protoplasts / Q000502:physiology; D013213:Starch / Q000378:metabolism; D017355:Zymomonas / Q000201:enzymology / Q000235:genetics*; D000516:alpha-Amylases / Q000096:biosynthesis*
chemical_list: D013213:Starch; D000516:alpha-Amylases