Assignment of the dipeptidylpeptidase IV (DPP4) gene to pig chromosome 15q21.
A porcine 2-kb partial dipeptidylpeptidase IV (DPP4, EC 3.4.14.5) cDNA clone and a porcine 16-kb genomic fragment containing parts of the DPP4 gene were isolated, characterized, and used as probes to map the DPP4 gene to pig Chr (Chr) 15q21 by fluorescence in situ hybridization. A two-allele RFLP was revealed for the DPP4 gene. This polymorphism was utilized in a linkage test against the erythrocyte antigen G (EAG), previously assigned to Chr 15, and the microsatellite S0088, which is linked to EAG. The linkage analyses revealed significant evidence for linkage confirming the assignment of DPP4 to Chr 15.
Thomsen
P D
PD
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Qvist
H
H
Marklund
L
L
Andersson
L
L
Sjöström
H
H
Norén
O
O
D000818:Animals; D002874:Chromosome Mapping; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000235:genetics*; D004912:Erythrocytes / Q000276:immunology; D008040:Genetic Linkage; D017404:In Situ Hybridization, Fluorescence; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000235:genetics*
D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4