Restriction fragment length polymorphisms of 16S rRNA genes in the differentiation of fast-growing mycobacterial species.
DNA from several species of fast growing mycobacteria displayed a characteristic restriction fragment length polymorphism (RFLP) pattern when hybridizated to a Mycobacterium fortuitum 16S rRNA gene fragment. The resulting patterns were identical when comparing different strains belonging to the same species. The RFLP results were consistent with those obtained by DNA-DNA hybridization studies. Using this approach, we have been able to identify the number of copies for 16S rRNA genes in several fast-growing mycobacteria.
Domenech
P
P
Departamento de Medicina Preventiva, Facultad de Medicina, Universidad Autonoma, Madrid, Spain.
Menendez
M C
MC
Garcia
M J
MJ
D001483:Base Sequence; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics* / Q000254:growth & development; D016926:Mycobacterium chelonae / Q000235:genetics / Q000254:growth & development; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013997:Time Factors
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S