Atrial natriuretic factor inhibits noradrenaline release in the presence of angiotensin II and III in the rat hypothalamus.
1. Atrial natriuretic factor effects on neuronal noradrenaline release evoked by angiotensin II or III and high potassium solution plus angiotensin II and III in the rat hypothalamus were studied. 2. Atrial natriuretic factor (10 nM) did not modify spontaneous noradrenaline release. On the other hand, the atrial factor diminished the increase of noradrenaline release induced by both angiotensin II (1 microM) or angiotensin III (1 microM). 3. Ten nanomolar ANF reduced the amine output induced by 100 nM KCl. Both angiotensins enhanced the 3H-noradrenaline secretion stimulated by high potassium solutions. When atrial natriuretic factor was added to the medium containing the depolarizing KCl solution plus angiotensin II or III (1 microM), the diminishing effects were greater than when the atrial factor was added to the depolarizing solution alone. 4. Our results suggest that atrial natriuretic factor effects on noradrenaline release, evoked by angiotensin II, III and KCl, may be involved in the regulation of the central catecholamine pathways and sympathetic activity.
Vatta
M S
MS
Catedra de Fisiopatologia and Programa de Sistemas Vasodepresores-Consejo Nacional de Investigaciones Cientificas y Tecnicas (PROSIVAD-CONICET), Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.
Papouchado
M L
ML
Bianciotti
L G
LG
Fernandez
B E
BE
D000804:Angiotensin II / Q000494:pharmacology*; D000805:Angiotensin III / Q000494:pharmacology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
C472610:atrial natriuretic peptide, rat; D000804:Angiotensin II; D000805:Angiotensin III; D009320:Atrial Natriuretic Factor; D011188:Potassium; D009638:Norepinephrine