Drought, heat and salt stress induce the expression of a citrus homologue of an atypical late-embryogenesis Lea5 gene.
In a search for genes that are induced in citrus cell suspension in response to salt stress, a cDNA clone with high homology to cotton Lea5 gene was isolated. Data base analysis of the protein deduced from the nucleotide sequence indicates that, like in cotton, the protein from citrus contains regions with significant hydropathic character. The gene, designated C-Lea5, is expressed in citrus leaves as well as cell suspension. The steady-state level of C-Lea5 is increased in cell suspension that is grown in the presence of 0.2 M NaCl. This phenomenon is also observed in leaves of citrus plants irrigated with NaCl and in citrus seedlings which are exposed to drought and heat stress. We suggest that the osmotic stress resulted from elevated level of salt is responsible for the increase in the level of C-Lea5.
Naot
D
D
Department of Fruit Tree Breeding and Genetics, Volcani Center, Bet Dagan, Israel.
Ben-Hayyim
G
G
Eshdat
Y
Y
Holland
D
D
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002957:Citrus / Q000235:genetics*; D003001:Cloning, Molecular; D003890:Desiccation; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D018749:RNA, Plant / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology
D000596:Amino Acids; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C066246:late embryogenesis abundant protein, plant; D012965:Sodium Chloride