Increased in vitro neutrophil adherence in a case of chronic idiopathic neutropenia.
In this report we describe a patient with persistent neutropenia whose neutrophils showed increased adhesion in a microplate assay. In three separate assays, from 12.5% to 13.7% of the patient's blood neutrophils exhibited spontaneous (unstimulated) adhesion to fetal bovine serum-coated microplate wells, much higher than adhesion of cells from healthy controls (1.9% +/- 2.5 SD, n = 20). The difference of spontaneous adhesion between the patient's and control neutrophils was even higher when cells from a skin-window exudate were examined (patient: 42.1-100% adhesion; control: 3.6% +/- 3.5 SD, n = 20). Over 80% inhibition of the increased adhesion was produced by the 60.3 anti-CD 18 monoclonal antibody, suggesting an involvement of beta 2-integrins.
Biasi
D
D
Istituto di Patologia Medica, University of Verona, Italy.
Bambara
L M
LM
Carletto
A
A
Caramaschi
P
P
Ortolani
R
R
Perona
G
G
Bellavite
P
P
D015703:Antigens, CD / Q000032:analysis; D018845:CD11 Antigens; D018821:CD18 Antigens; D002448:Cell Adhesion; D002908:Chronic Disease; D005122:Exudates and Transudates / Q000166:cytology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009503:Neutropenia / Q000097:blood / Q000276:immunology / Q000473:pathology*; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism
D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; D013481:Superoxides