title: Characterization of two groEL genes in Streptomyces coelicolor A3(2).
abstract: Two Streptomyces coelicolor A3(2) groEL-like genes, groEL1 and groEL2, were cloned and characterized. Pulsed-field-gel electrophoresis located these genes, which were not adjacent, in the same segment of the chromosome. Nucleotide sequence analysis revealed that groEL1, but not groEL2, was preceded by a groES-like gene. Northern blots showed that heat shock induced the accumulation of transcripts corresponding to groES (0.7 kb), groES/EL1 (2.3 kb) and groEL2 (2.1 kb). Unique transcription start points and promoters were located for groES/EL1 and groEL2, having -10 and -35 hexamers similar to eubacterial vegetative promoters. Regions located 5' to the groES/EL1 or groEL2 structural genes contain 'GCACTCN9GAGTGC' motifs conserved upstream from the heat-shock genes of other bacteria.
authors.affiliation: Unité de Génie Microbiologique, Institut Pasteur, Paris, France.
authors.forename: A M
authors.initials: AM
authors.lastname: Duchêne
authors.forename: H M
authors.initials: HM
authors.lastname: Kieser
authors.forename: D A
authors.initials: DA
authors.lastname: Hopwood
authors.forename: C J
authors.initials: CJ
authors.lastname: Thompson
authors.forename: P
authors.initials: P
authors.lastname: Mazodier
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018834:Chaperonin 60; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D012333:RNA, Messenger