title: A Macintosh program for result analysis in HLA genotyping by the modified PCR-RFLP method.
abstract: We describe in this report a computer program for easy interpretation of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) patterns generated during HLA Class II typing by the modified PCR-RFLP method. HLA-DQA1, HLA-DQB1, HLA-DRB1 and HLA-DPB1 typing result analysis are thus greatly simplified. The program also allows video capture and image storage, which are becoming a new standard in molecular biology laboratories.
authors.affiliation: Centre Régional de Transfusion Sanguine de Bordeaux, France.
authors.forename: P
authors.initials: P
authors.lastname: Merel
authors.forename: V
authors.initials: V
authors.lastname: Pigeonnier
authors.forename: F
authors.initials: F
authors.lastname: Comeau
authors.forename: G
authors.initials: G
authors.lastname: Vezon
mesh_terms: D005838:Genotype; D006680:HLA Antigens / Q000235:genetics*; D006682:HLA-DP Antigens / Q000235:genetics; D059868:HLA-DP beta-Chains; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012984:Software
chemical_list: D006680:HLA Antigens; D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II