Ultraviolet irradiation of murine skin alters cluster formation between lymph node dendritic cells and specific T lymphocytes.
Exposure of murine skin to suberythemal doses of ultraviolet-B (UVB) radiation before contact sensitization alters the activity of antigen-presenting cells (APC) in the draining lymph nodes (DLN), decreases the contact hypersensitivity (CHS) response, and induces hapten-specific Ts cells. We determined whether in vivo UVB irradiation alters the ability of hapten-bearing APC from the DLN to form clusters with hapten-specific T lymphocytes. When APC from UV-irradiated, fluorescein isothiocyanate (FITC)-sensitized mice were mixed with a FITC-specific T-cell line, significantly fewer clusters formed compared with FITC+ APC from unirradiated mice. A higher percentage of clusters formed with APC from UV-irradiated mice were Mac-1+ and bound both CD4+ and CD8+ T cells, whereas FITC+ APC from nonirradiated mice bound CD4+ cells. These results suggest that UVB irradiation interferes with the induction of CHS by altering the functional interaction between APC and T cells, perhaps by altering the population of APC in the skin.
Muller
H K
HK
Department of Pathology, University of Tasmania, Hobart, Australia.
Bucana
C D
CD
Kripke
M L
ML
Cox
P A
PA
Saijo
S
S
Strickland
F M
FM
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000528:radiation effects; D016827:CD8 Antigens / Q000032:analysis; D002449:Cell Aggregation / Q000528:radiation effects*; D002460:Cell Line; D003713:Dendritic Cells / Q000528:radiation effects*; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology* / Q000528:radiation effects; D016177:Macrophage-1 Antigen / Q000032:analysis; D051379:Mice; D008809:Mice, Inbred C3H; D008854:Microscopy, Electron; D012867:Skin / Q000528:radiation effects; D016176:T-Lymphocyte Subsets / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D016827:CD8 Antigens; D016177:Macrophage-1 Antigen; D016650:Fluorescein-5-isothiocyanate