title: Characterization of the murF gene of the cyanobacterium Synechocystis sp. PCC 6803.
abstract: The murF gene encodes UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2, 6-diaminopimeloyl-D-Ala-D-Ala synthetase (EC 6.3.2.15), which catalyses the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein. An open reading frame identified as the murF gene was found in the genome of the cyanobacterium Synechocystis sp. PCC 6803. The murF gene encodes a polypeptide of 454 amino acid residues with a predicted molecular mass of 48 kDa. The murF gene is present as a single-copy gene in the genome of Synechocystis sp. PCC 6803. The amino acid sequence deduced from the murF gene is 39% identical to that of the product of the murF gene of Escherichia coli. The cyanobacterial murF gene complemented a temperature-sensitive mutation in a murF-deficient strain of E. coli, restoring the mutant's ability to synthesize a cell wall and to survive at high sublethal temperatures.
authors.affiliation: Department of Regulation Biology, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: M P
authors.initials: MP
authors.lastname: Malakhov
authors.forename: D A
authors.initials: DA
authors.lastname: Los
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: V E
authors.initials: VE
authors.lastname: Semenenko
authors.forename: N
authors.initials: N
authors.lastname: Murata
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016366:Open Reading Frames; D010453:Peptide Synthases / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D011994:Recombinant Proteins; D010453:Peptide Synthases; C060628:UDP-N-acetylmuramoylalanyl-D-glutamyllysine-D-alanyl-D-alanine ligase