title: Radial glial cell transformation to astrocytes is bidirectional: regulation by a diffusible factor in embryonic forebrain.
abstract: During development of mammalian cerebral cortex, two classes of glial cells are thought to underlie the establishment of cell patterning. In the embryonic period, migration of young neurons is supported by a system of radial glial cells spanning the thickness of the cortical wall. In the neonatal period, neuronal function is assisted by the physiological support of a second class of astroglial cell, the astrocyte. Here, we show that expression of embryonic radial glial identity requires extrinsic soluble signals present in embryonic forebrain. Moreover, astrocytes reexpress features of radial glia in vitro in the presence of the embryonic cortical signals and in vivo after transplantation into embryonic neocortex. These findings suggest that the transformation of radial glia cells into astrocytes is regulated by availability of inducing signals rather than by changes in cell potential.
authors.affiliation: Laboratory of Developmental Neurobiology, Rockefeller University, New York, NY 10021.
authors.forename: K E
authors.initials: KE
authors.lastname: Hunter
authors.forename: M E
authors.initials: ME
authors.lastname: Hatten
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000502:physiology; D001685:Biological Factors / Q000502:physiology; D015153:Blotting, Western; D016380:Brain Tissue Transplantation / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004622:Embryo, Mammalian; D016332:Fetal Tissue Transplantation / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D008564:Membrane Potentials; D051379:Mice; D008959:Models, Neurological; D009457:Neuroglia / Q000166:cytology* / Q000502:physiology; D016548:Prosencephalon / Q000166:cytology / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D001685:Biological Factors; D005904:Glial Fibrillary Acidic Protein