title: Elongation factor 1 alpha is a component of the subcortical actin bundles of characean algae.
abstract: Antibodies to elongation factor 1 alpha (EF1 alpha), a known 50 kDa actin-bundling protein in Dictyostelium, identified a protein in a whole cell extract of the characean alga Nitella pseudoflabellata that had an apparent molecular weight of 51 kDa. Indirect immunofluorescence microscopy revealed labelling by the EF1 alpha antibodies of the subcortical actin bundles, even after the motile organelles of the endoplasm were removed by perfusion with ATP-containing solutions.
authors.affiliation: Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, Canberra.
authors.forename: D A
authors.initials: DA
authors.lastname: Collings
authors.forename: G O
authors.initials: GO
authors.lastname: Wasteneys
authors.forename: M
authors.initials: M
authors.lastname: Miyazaki
authors.forename: R E
authors.initials: RE
authors.lastname: Williamson
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D003595:Cytoplasmic Streaming; D056890:Eukaryota / Q000378:metabolism* / Q000648:ultrastructure; D046911:Macromolecular Substances; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D015388:Organelles / Q000737:chemistry; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D000199:Actins; D046911:Macromolecular Substances; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010940:Plant Proteins