title: Transmission of maternal blood cells to the fetus during pregnancy: detection in mouse neonatal spleen by immunofluorescence flow cytometry and polymerase chain reaction.
abstract: Mouse embryos were transferred to allogeneic pseudopregnant foster mothers and the cells of the resultant neonates were analyzed for the expression of maternal major histocompatibility complex (MHC) antigens to elucidate maternal cell transmission to the fetus during pregnancy. Expression of maternal-type MHC antigens was detected in the spleen but not in the liver or thymus of the neonates in analyses by immunofluorescence flow cytometry and polymerase chain reaction. These findings provide evidence, on the molecular basis, that maternal blood cells are transmitted to the fetus through the placenta.
authors.affiliation: Laboratory of Molecular Immunology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shimamura
authors.forename: S
authors.initials: S
authors.lastname: Ohta
authors.forename: R
authors.initials: R
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000954:Antigens, Surface / Q000032:analysis / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique; D006183:H-2 Antigens / Q000032:analysis / Q000235:genetics*; D007438:Introns; D008285:Major Histocompatibility Complex; D008431:Maternal-Fetal Exchange / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D011555:Pseudopregnancy; D013154:Spleen / Q000276:immunology*; D018800:Thy-1 Antigens
chemical_list: D000954:Antigens, Surface; D017931:DNA Primers; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens