The duplicated gene copy of the ovine growth hormone gene contains a PvuII polymorphism in the second intron.
PvuII restriction fragment length polymorphism (RFLP) was found at the growth hormone locus in sheep carrying the GH2 allele where the gene is duplicated. By restriction analysis and using the polymerase chain reaction we demonstrated that this RFLP is due to a mutation at the PvuII site located in the second intron of the 3' copy of the GH2 allele.
Gootwine
E
E
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Sise
J A
JA
Penty
J M
JM
Montgomery
G W
GW
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D013006:Growth Hormone / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012756:Sheep
D017931:DNA Primers; D013006:Growth Hormone; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific