Human serum albumin conformational changes as induced by tenoxicam and modified by simultaneous diazepam binding.
The binding of tenoxicam to human serum albumin has been shown by affinity chromatography proton titration and equilibrium dialysis to be dependent on the neutral to basic conformational change of the protein. The influence of diazepam on the interaction was also investigated using the same techniques, suggesting that diazepam increases the association of tenoxicam to albumin. Affinity chromatography revealed that the reciprocal effect also occurs. Displacement studies indicated that diazepam causes a significant increase in the affinity of tenoxicam to its main binding site, albumin site I, which is different from the diazepam site (site II). Tenoxicam seemed to cause an allosteric change in the conformation of the protein during its own binding, as did warfarin. The mechanism of this effect was a pH-dependent conformational change of albumin induced by electrostatic forces within the protein. Diazepam induced a distant accommodation of the protein, an effect accompanied by an enhanced inhibition of the release of protons from albumin.
Brée
F
F
Département de Pharmacologie, Faculté de Médecine, Créteil, France.
Urien
S
S
Nguyen
P
P
Tillement
J P
JP
Steiner
A
A
Vallat-Molliet
C
C
Testa
B
B
Visy
J
J
Simonyi
M
M
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism* / Q000494:pharmacology*; D002846:Chromatography, Affinity; D003956:Dialysis; D003975:Diazepam / Q000378:metabolism* / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010894:Piroxicam / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D012709:Serum Albumin; D010894:Piroxicam; D003975:Diazepam; C032801:tenoxicam