title: Evaluation of a 22 kDa Dirofilaria immitis antigen for the immunodiagnosis of human pulmonary dirofilariosis.
abstract: Human pulmonary dirofilariosis usually appears as a solitary pulmonary nodule. A 22 kDa antigen from Dirofilaria immitis adult worms specifically recognized by three sera from individuals diagnosed with pulmonary dirofilariosis was identified by enzyme-linked-immunoelectrotransfer blot. This antigen was purified by elution from acrylamide gels and evaluated in enzyme-linked immunoassay using sera from 92 patients with pulmonary dirofilariosis and other pulmonary and parasitic diseases. Its use in the diagnosis of human pulmonary dirofilariosis should be considered.
authors.affiliation: Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Perera
authors.forename: A
authors.initials: A
authors.lastname: Muro
authors.forename: M
authors.initials: M
authors.lastname: Cordero
authors.forename: E
authors.initials: E
authors.lastname: Villar
authors.forename: F
authors.initials: F
authors.lastname: Simón
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D004183:Dirofilaria immitis / Q000276:immunology*; D004184:Dirofilariasis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008174:Lung Diseases, Parasitic / Q000175:diagnosis*; D012698:Serologic Tests / Q000379:methods
chemical_list: D000947:Antigens, Helminth