Application of radioreceptor assays for systematic toxicological analysis--1. Procedures for radioreceptor assays for antihistaminics, anticholinergics and benzodiazepines.
Radioreceptor assays can be a useful tool for systematic toxicological analysis in that they can be applied for the detection of an entire pharmacological class of drugs. In the present paper procedures for radioreceptor assays for benzodiazepines, anticholinergics and antihistaminics have been described in detail. The development of the assay for antihistaminics in urine is given in order to illustrate the prerequisites for these types of assays with regard to the incubation conditions. In part 2 the applicability of the three assays for systematic toxicological analysis will be evaluated on the basis of testing a large number of urine samples after administration of a selected number of drugs to healthy volunteers and patients.
Ensing
K
K
Department of Analytical Chemistry and Toxicology, University Centre for Pharmacy, Groningen, The Netherlands.
Bosman
I J
IJ
Egberts
A C
AC
Franke
J P
JP
de Zeeuw
R A
RA
D000818:Animals; D001569:Benzodiazepines / Q000008:administration & dosage / Q000652:urine*; D001665:Binding Sites; D005189:False Positive Reactions; D006633:Histamine Antagonists / Q000008:administration & dosage / Q000652:urine*; D006634:Histamine H1 Antagonists / Q000008:administration & dosage / Q000652:urine; D006635:Histamine H2 Antagonists / Q000008:administration & dosage / Q000652:urine; D006801:Humans; D010276:Parasympatholytics / Q000008:administration & dosage / Q000652:urine*; D011869:Radioligand Assay / Q000379:methods*; D014116:Toxicology / Q000379:methods*
D006633:Histamine Antagonists; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D010276:Parasympatholytics; D001569:Benzodiazepines