[Regulation of transcription of the interleukin-2 gene in B-lymphocytes].
Since most B cell clones immortalized with EBV virus can be induced to produce interleukin-2, a typical T cell cytokine, we studied the role of different elements of the IL-2 promoter in such clones by transfection. It was found, in particular, that the element TCEd, which binds the transcription factor NF-kB, is very active in all three B clones tested. This element has no activity in T cells of the Jurkat line. The NFATd element, which binds the transcription factor NFAT-1 and is very active in T cells, is only weakly active in one B clone and not at all in another. Different elements thus contribute to IL-2 promoter activity in different cells.
Mouzaki
A
A
Département de médecine, Hôpital cantonal universitaire de Genève.
Zubler
R H
RH
D001402:B-Lymphocytes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics; D004854:Herpesvirus 4, Human; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D008081:Liposomes / Q000494:pharmacology; D016328:NF-kappa B / Q000235:genetics; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins / Q000235:genetics; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D004268:DNA-Binding Proteins; D007376:Interleukin-2; D008081:Liposomes; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D010714:Phosphatidylethanolamines; D014157:Transcription Factors; C020888:1,2-dielaidoylphosphatidylethanolamine
Régulation de la transcription du gène de l'interleukin-2 dans les lymphocytes B.