Genetic diversity among Borrelia burgdorferi isolates from wood rats and kangaroo rats in California.
Twenty-nine Borrelia burgdorferi isolates, obtained from dusky-footed wood rats (Neotoma fuscipes) and California kangaroo rats (Dipodomys californicus) in California, were analyzed genetically. Chromosomal DNA was examined by restriction endonuclease analysis (REA) and gene probe restriction fragment length polymorphism. Pulsed-field gel electrophoresis was used to analyze the plasmid profiles of the isolates. REA, the method with the greatest discrimination, disclosed 24 distinct restriction patterns among the 29 isolates. These restriction patterns were sorted into four restriction fragment length polymorphism groups on the basis of their gene hybridization patterns. Results of the REA and plasmid profile analysis supported this grouping. The degree of genetic diversity among Californian isolates demonstrated by our findings is greater than that previously reported among other groups of North American isolates and is similar or greater than the diversity reported among European isolates.
Zingg
B C
BC
Department of Entomological Sciences, University of California, Berkeley 94720.
Brown
R N
RN
Lane
R S
RS
LeFebvre
R B
RB
D000818:Animals; D015748:Borrelia burgdorferi Group / Q000235:genetics* / Q000302:isolation & purification*; D002140:California; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004173:Dipodomys / Q000382:microbiology*; D004199:Disease Vectors; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D010957:Plasmids / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D016205:Sigmodontinae / Q000382:microbiology*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes