Association of chromosomal telomere DNA with nuclear matrix in HeLa cell.
Using Electron Spectroscopic Imaging (ESI), we visualized the in situ binding of nucleic acids to nuclear matrix and 3H-thymidine incorporation which indicates that a small partial DNA bound to nuclear matrix tightly. Furthermore we found that chromosomal telomere DNA could bind to nuclear matrix specifically by the dot and Southern hybridization. The result of the Southwestern blot suggests that telomere DNA has high affinity to lamin B, vimentin and some nuclear matrix proteins. Therefore, the nuclear matrix and lamina of HeLa cell are possibly associated with spatial organization and action of chromosome.
Wang
G S
GS
Department of Biology, Peking University, Beijing, PRC.
Luo
W J
WJ
Pan
W J
WJ
Ding
M X
MX
Zhai
Z H
ZH
D004247:DNA / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D034921:Lamin Type B; D034882:Lamins; D015530:Nuclear Matrix / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D016615:Telomere / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism
D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; D014746:Vimentin; D004247:DNA