title: A novel structural form of the 2 micron plasmid of the yeast Saccharomyces cerevisiae.
abstract: DNA was isolated from cells of Saccharomyces cerevisiae incubated under conditions that enriched for DNA replication intermediates. A novel form of the 2 microns plasmid was detected, in which two monomeric or dimeric circles were joined by a linear double-stranded segment of variable length. We suggest that this molecule is a consequence of site-specific recombination within a dimeric DNA molecule during DNA replication. The existence of this molecule provides supporting physical evidence for a variant of the model of 2 mu plasmid amplification first proposed by Futcher (1986).
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
authors.forename: T D
authors.initials: TD
authors.lastname: Petes
authors.forename: D H
authors.initials: DH
authors.lastname: Williamson
mesh_terms: D004261:DNA Replication; D010957:Plasmids; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*