Rapid identification of the common apo E isoform genotype using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP).
Polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) was used to identify the common apoprotein E (apo E) isoform genotypes. DNA from the target sequence on apo E was amplified by PCR from peripheral blood genomic DNA. Non-radiolabelled natural PCR products were electrophoresed on a polyacrylamide gradient gel and automatically silver-stained. Bands compatible with each of the three common apo E isoforms were obtained. This method, which does not require restriction enzymes or DNA purification, is a rapid and useful means of detecting the apo E isoform genotype.
Aozaki
R
R
Genetic Research Laboratory, SRL, Tokyo, Japan.
Kawaguchi
R
R
Ogasa
U
U
Hikiji
K
K
Kubo
N
N
Sakurabayashi
I
I
D001057:Apolipoproteins E / Q000235:genetics*; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000032:analysis / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007536:Isomerism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D001057:Apolipoproteins E; D004277:DNA, Single-Stranded