Direct mapping of seven genes encoding human type 2 cystatins to a single site located at 20p11.2.
The human type 2 cystatins are encoded by a multigene family of eight to nine members. Five functional genes (CST1-CST5) and two pseudogenes (CSTP1 and CSTP2) have previously been cloned. We have developed a panel of short genomic fragments that serve as gene-specific probes for these seven genes under hybridization conditions used for Southern blots or fluorescence in situ hybridization (FISH). Analysis of BssHII-digested genomic DNA using these probes was consistent with association of all seven genes with a 905-kb fragment, suggesting physical clustering at a single site. The cluster was assigned to chromosome 20 by screening a somatic cell hybrid panel with an STS for CST2. This assignment was confirmed for each gene by FISH using repeat-free gene-specific genomic probes ranging in size from 2.3 to 0.8 kb. All the loci mapped to 20p11.2.
Dickinson
D P
DP
Department of Basic Sciences, University of Texas-Houston Health Science Center 77225.
Zhao
Y
Y
Thiesse
M
M
Siciliano
M J
MJ
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002874:Chromosome Mapping; D002890:Chromosomes, Human, Pair 20; D006224:Cricetinae; D015891:Cystatins / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data
D015891:Cystatins; D017931:DNA Primers