Immunological differentiation of human tissue-nonspecific type alkaline phosphatases by a monoclonal antibody to the enzyme of human osteoblast-like cells.
Monoclonal antibodies against alkaline phosphatase [ALP; ortho-phosphoric monoester phosphohydrolase, alkaline optimum, EC 3.1.3.1.] of cultured human osteoblast-like cells (HBC) were raised in mice. Immuno-reactions of tissue-nonspecific type ALP from human bone, dental pulp, liver and kidney as well as intestinal and placental types to the monoclonal antibodies were compared by a dot immunoassay and ELISA. One clone was able to recognize antigenic differences among tissue-nonspecific type ALPs in addition to intestinal and placental ALPs; it reacted favorably with ALPs from HBC, human bone, kidney and dental pulp, but not with human liver enzyme. Similarly, the antibody immunoreacted with bone-derived ALP but not with liver-derived enzyme present in human serum. The present monoclonal antibody preparation can be utilized in basic studies as well as in clinical laboratory tests to distinguish minor heterogeneity among human ALPs.
Goseki
M
M
Department of Biochemistry, Tokyo Medical and Dental University.
Oida
S
S
Ogata
Y
Y
Sasaki
S
S
D000469:Alkaline Phosphatase / Q000032:analysis / Q000145:classification / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001842:Bone and Bones; D002478:Cells, Cultured; D003429:Cross Reactions; D003782:Dental Pulp; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007422:Intestines; D007668:Kidney; D008099:Liver; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity / Q000276:immunology; D010006:Osteoblasts / Q000737:chemistry / Q000201:enzymology* / Q000276:immunology*; D010920:Placenta; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase