Species identification of trichostrongyle nematodes by PCR-linked RFLP.
Restriction fragment length polymorphisms (RFLP) of the second internal transcribed spacer (ITS-2) of ribosomal DNA were examined for 6 species of ruminant nematodes; Trichostrongylus colubriformis, T. axei, T. vitrinus, Haemonchus contortus, Teladorsagia circumcincta and Cooperia oncophora. The ITS-2 of each species was amplified by PCR and restricted with the endonucleases DraI, HinfI, RsaI and VspI. Using this approach, the 6 species could be distinguished. No intraspecific variation in RFLP's has been detected so far in the ITS-2 of these species, suggesting that this technique has considerable potential for the identification of eggs of different nematode species in faecal samples.
Gasser
R B
RB
Department of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Chilton
N B
NB
Hoste
H
H
Stevenson
L A
LA
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004275:DNA, Ribosomal / Q000032:analysis* / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012418:Ruminants / Q000469:parasitology*; D014251:Trichostrongyloidea / Q000235:genetics / Q000302:isolation & purification*; D014252:Trichostrongyloidiasis / Q000469:parasitology / Q000662:veterinary*
D017931:DNA Primers; D004275:DNA, Ribosomal