[Group A streptococcal polysaccharide--an inhibitor of the functional activity of T-suppressors induced by Con A in a culture of mononuclear cells of human peripheral blood].
It was found that group A streptococcal polysaccharide (A-PS) had no mitogenic effect on the intact human blood mononuclear cells' culture (MNC) and on its proliferation stimulated with ConA. Using a double staining technique for simultaneously determining cell surface phenotype and degree of cell activation by it's ability to include the tetrazolium dye MTT (3-[4,5-dimethylthiazol-2-yl] -2,5-diphenyl tetrazolium bromide) it was established that A-PS decreased the percent of activated (MTT+) cells in the subpopulation of CD8+ and increased the percent of MTT+ cells among CD4+ lymphocytes in the intact MNC. In the MNC stimulated with ConA A-PS caused only one of these effects: it decreased the percent of MTT+ cells in the subpopulation of CD8+ lymphocytes. The correlation was established between the results obtained with MTT-technique and cell transfer test, because the MNC preincubated with ConA and A-PS lost the ability to suppresses MNC proliferation stimulated with PHA. The data obtained supported the assumption that A-PS as a carrier of determinants common with thymus epidermal antigens (factors) may act as it's functional analogue and thus promote the development of autoimmune process during the rheumatic fever.
Gnezitskaia
E V
EV
Bazanova
E A
EA
Nesterenko
V G
VG
Popova
L K
LK
Sanina
V Iu
VIu
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D003208:Concanavalin A / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D012016:Reference Values; D013297:Streptococcus pyogenes; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*; D013778:Tetrazolium Salts; D013844:Thiazoles
D004396:Coloring Agents; D011135:Polysaccharides, Bacterial; D013778:Tetrazolium Salts; D013844:Thiazoles; C036112:streptococcal polysaccharide group A; D003208:Concanavalin A; C022616:thiazolyl blue
Polisakharid streptokokka gruppy A--ingibitor funktsional'noĭ aktivnosti T-supressorov, indutsirovannykh KonA v kul'ture mononuklearnykh kletok perifericheskoĭ krovi cheloveka.