title: Polymerase chain reaction based cDNA cloning of wheat profilin: a potential plant allergen.
abstract: Highly degenerate profilin-specific primers were used to amplify by polymerase chain reaction the profilin-coding sequence of wheat cDNA. Analyzing the sequencing data after subcloning revealed the existence of three isoforms. The high amino acid sequence identity with the birch pollen allergen Betv II suggests that wheat profilin may be a potential plant allergen, relevant in food allergy.
authors.affiliation: Abteilung Molekulare Genetik, Berufsgenossenschaftliches, Forschungsinstitut für Arbeitsmedizin, Ruhr-Universität, Bochum, Deutschland.
authors.forename: H P
authors.initials: HP
authors.lastname: Rihs
authors.forename: P
authors.initials: P
authors.lastname: Rozynek
authors.forename: K
authors.initials: K
authors.lastname: May-Taube
authors.forename: B
authors.initials: B
authors.lastname: Welticke
authors.forename: X
authors.initials: X
authors.lastname: Baur
mesh_terms: D000485:Allergens / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003285:Contractile Proteins; D018076:DNA, Complementary / Q000032:analysis; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051304:Profilins; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000235:genetics*
chemical_list: D000485:Allergens; D003285:Contractile Proteins; D018076:DNA, Complementary; D008840:Microfilament Proteins; D010940:Plant Proteins; D051304:Profilins