Properties of human albumin microparticles prepared by a chilled cross-linking technique.
Examination of conditions needed to form albumin microparticles (in the 1-5 microns diam. size range) by glutaraldehyde cross-linking, at low temperature (-15 degrees C), suggested that the particles formed very rapidly over a short time scale and at low concentrations of cross-linking reagent. Detailed analysis showed that the particles increased in size with time of reaction and with an increase in glutaraldehyde concentration. Evaluation of the particle matrix using a dilute trypsin digestion process suggested that the above factors may influence the internal composition since the particles dissolved less rapidly than might be anticipated from a consideration of particle diameter alone.
Oner
L
L
Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago 60607.
Groves
M J
MJ
D000418:Albumins / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008863:Microspheres; D009391:Nephelometry and Turbidimetry; D010316:Particle Size; D014357:Trypsin / Q000737:chemistry
D000418:Albumins; D003432:Cross-Linking Reagents; D014357:Trypsin; D005976:Glutaral