[Design of a chromosomal DNA probe species specific for Yersinia pestis].
A 14.8 kb DNA fragment from the chromosome of Yersinia pestis TWJ was cloned and the restriction map constructed. The fragment designated as T16 and its subfragments were tested in dot-hybridization with strains of Yersinia genus and other members of Enterobacteriaceae. A species-specific DNA probe (designated MK) was constructed on the basis of the T16 fragment. As judged from restriction analysis, blot-hybridization experiments and, partially, sequencing, significant homology exists between the MK DNA probe and this one developed by Bardarov et. al. (1990). A repeated sequence in two copies was discovered in the MK fragment.
Norkina
O V
OV
Kulichenko
A N
AN
Shovadaeva
G A
GA
Boshnakov
R Kh
RKh
Aksenov
M Iu
MIu
Popov
Iu A
IuA
Tomov
A Ts
ATs
K''esovskiĭ
V K
VK
Drozdov
I G
IG
Gintsburg
A L
AL
D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D013045:Species Specificity; D015010:Yersinia pestis / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial
Konstruirovanie vidospetsifichnogo dlia Yersinia pestis khromosomnogo DNA-zonda.