Evidence for involvement of protein kinase C in expression of intracellular adhesion molecule-1 (ICAM-1) by human vascular endothelial cells.
Potent activators of protein kinase C (PKC), such as phorbol dibutyrate and octylindolactam V, stimulated expression of intercellular adhesion molecule 1 (ICAM-1) by human umbilical vein endothelial cells (HUVEC) in a concentration-dependent manner. Expression of PKC activator-induced ICAM-1 in HUVEC was inhibited by the PKC inhibitor, H-7. Furthermore, cytokine (TNF alpha, LPS)-induced ICAM-1 expression was inhibited by the potent PKC inhibitor, H-7, and not by the cAMP-dependent protein kinase (PKA) specific inhibitor, H-89. These data suggest that PKC is involved in cytokine- and inflammatory agent-induced upregulation of ICAM-1 expression in HUVEC.
Sung
C P
CP
Department of Cardiovascular Pharmacology and Renal Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pa 19406.
Arleth
A J
AJ
Nambi
P
P
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1; D007546:Isoquinolines / Q000494:pharmacology; D007769:Lactams / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D047428:Protein Kinase Inhibitors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015854:Up-Regulation
D015815:Cell Adhesion Molecules; D007211:Indoles; D007546:Isoquinolines; D007769:Lactams; D008070:Lipopolysaccharides; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D014409:Tumor Necrosis Factor-alpha; C086014:7-octylindolactam V; D018799:Intercellular Adhesion Molecule-1; D015240:Phorbol 12,13-Dibutyrate; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C