Molecular cloning and polymerase chain reaction-sequence-specific oligonucleotide detection of the allele encoding the novel allospecificity HLA-Cw6.2 (Cw*1502) in Spanish gypsies.
A proposed novel allospecificity, HLA-Cw6.2, has been reported to be commonly found (approximately 25%) in Spanish Gypsies. Full-length cDNAs of the allele (Cw*1502) coding for this antigen have been cloned in this study. A simple PCR-SSO method for its detection has been standardized and a correlation with the serologic Cw6.2 phenotype has been established. This specificity has been also detected in the homozygous typing cell RML. Although the primary structures of Cw*1502 and Cw*0601 are not closely related, they share specific motifs that may account for their serologic cross-reactivity. Two novel HLA-C alleles (Cw*12022 and Cw*0602) are also reported.
Vilches
C
C
Immunology Department, Puerta de Hierro Hospital, Madrid, Spain.
de Pablo
R
R
Herrero
M J
MJ
Moreno
M E
ME
Kreisler
M
M
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004247:DNA / Q000032:analysis; D015236:HLA-C Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D016130:Immunophenotyping; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D017434:Protein Structure, Tertiary; D006178:Romani People / Q000235:genetics*; D013030:Spain
D015236:HLA-C Antigens; C528994:HLA-C*06 antigen; D009841:Oligonucleotides; D004247:DNA