Lack of relationship between gamma-glutamyl transpeptidase and tyrosine transport in cultured bovine adrenal chromaffin cells.
Effect of gamma-glutamyl transpeptidase (GGTP) inhibitor acivicin on tyrosine transport was investigated in cultured bovine adrenal chromaffin cells. Tyrosine transport into the cells was inhibited by acivicin at the low concentration range. This drug also caused the inhibition of GGTP in the cell lysates, but the concentration required for the inhibition of the enzyme was evidently different from that producing the inhibition of tyrosine transport into the cells. In addition, tyrosine transport was not affected by a GGTP activator hippurate even at the high concentration. These results seem to provide the evidence that GGTP may not be involved in the transport of tyrosine into the adrenal chromaffin cell.
Morita
K
K
Department of Pharmacology, Tokushima University School of Medicine, Japan.
Teraoka
K
K
Hamano
S
S
Oka
M
M
D000311:Adrenal Glands / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured; D006626:Hippurates / Q000494:pharmacology; D007555:Isoxazoles / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism*; D005723:gamma-Glutamyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D006626:Hippurates; D007555:Isoxazoles; D014443:Tyrosine; D005723:gamma-Glutamyltransferase; C006303:acivicin; C030514:hippuric acid