Blotting membrane micropreparation in capillary electrophoresis to evaluate enzyme purity and activity.
A micropreparation system for proteins and peptides analyzed by capillary electrophoresis that uses a blotting membrane and an improved micro-test-plate was developed. The system is useful for enzyme diagnosis and purification. In the system, enzyme is collected in a concentrated spot on these membranes, and the activities are measured enzymatically to evaluate the resolution of the system.
Konse
T
T
Analytical and Metabolic Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Takahashi
T
T
Nagashima
H
H
Iwaoka
T
T
D002197:Capillary Action; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D004798:Enzymes / Q000302:isolation & purification* / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D007202:Indicators and Reagents; D007700:Kinetics; D008567:Membranes, Artificial; D005723:gamma-Glutamyltransferase / Q000302:isolation & purification* / Q000378:metabolism*
D004798:Enzymes; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D006160:Guanosine Triphosphate; D005723:gamma-Glutamyltransferase