High resolution localization of recombination hot spots using sperm typing.
We have applied sperm DNA typing to determine the distribution of crossover events within a one megabase region of the short arm of human chromosome 4 near the locus for Huntington disease. A total of 29 recombinants were detected among 602 sperm typed after whole genome amplification. These recombinants were typed for seven polymorphic markers. The 280 kilobase D4S10-D4S126 interval was found to undergo recombination at a 6-9-fold greater rate per unit of physical distance than the adjacent 720 kb D4S126-D4S127 interval. Sperm typing has the potential to dissect mammalian recombination hot spots to the point where DNA sequence analysis may reveal the molecular basis for hyperrecombination.
Hubert
R
R
Molecular Biology Program, University of Southern California, Los Angeles 90089-1340.
MacDonald
M
M
Gusella
J
J
Arnheim
N
N
D001483:Base Sequence; D002894:Chromosomes, Human, Pair 4; D003434:Crossing Over, Genetic; D004247:DNA / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D006816:Huntington Disease / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013094:Spermatozoa
D005819:Genetic Markers; D004247:DNA