Cu-ATSM, an intracellular-accessible superoxide dismutase (SOD)-like copper complex: evaluation in an ischemia-reperfusion injury model.
We have reported a stable superoxide dismutase (SOD)-like copper complex, Cu-ATSM, which shows high membrane permeability and distribution to the brain or heart. In this study, we evaluated the protective effects of Cu-ATSM on superoxide-mediated tissue damage caused by ischemia-reperfusion using an isolated perfused rat heart model. Lipid peroxidation levels in the Cu-ATSM treated group were lower than those in the non-treated group. Furthermore, released creatine phosphokinase into the perfusate, a marker of tissue damage, was reduced by Cu-ATSM treatment. These results indicated the possibility of Cu-ATSM being an effective SOD-like drug for the treatment of superoxide-mediated damage, such as ischemia-reperfusion injury.
Wada
K
K
Department of Radiopharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Fujibayashi
Y
Y
Tajima
N
N
Yokoyama
A
A
D000818:Animals; D056831:Coordination Complexes; D003326:Coronary Circulation / Q000187:drug effects; D003402:Creatine Kinase / Q000378:metabolism; D016166:Free Radical Scavengers; D006439:Hemodynamics / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D017202:Myocardial Ischemia / Q000188:drug therapy / Q000503:physiopathology*; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy / Q000503:physiopathology*; D009206:Myocardium / Q000201:enzymology; D009942:Organometallic Compounds / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000494:pharmacology*; D013481:Superoxides / Q000633:toxicity; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013882:Thiosemicarbazones / Q000494:pharmacology*
D056831:Coordination Complexes; D016166:Free Radical Scavengers; D009942:Organometallic Compounds; D017392:Thiobarbituric Acid Reactive Substances; D013882:Thiosemicarbazones; C089548:copper (II) diacetyl-di(N(4)-methylthiosemicarbazone); D013481:Superoxides; D013482:Superoxide Dismutase; D003402:Creatine Kinase