Production and characterization of a new monoclonal antibody, GR-ICOR-2, recognizing sarcomeric actin: analysis of the expression in the developing chick heart.
We produced and characterized a specific monoclonal antibody (mAB) designated GR-ICOR-2. This mAb recognizes sarcomeric actin molecules (43 kDa) and was used in an immunohistochemical analysis of staining patterns in Hamburger and Hamilton's stages 18, 22 and 25 (HH 18, 22 and 25) embryonic chick hearts. Staining showed a mainly cytoplasmic distrubition in three regions: the atrioventricular (AV) canal cushion tissue, the primitive ventricle, and conal crests. In addition, this mAb-cross-reacted with rabbit and human cardiac and skeletal muscle tissue; but not with smooth muscle tissue.
Fernández
J E
JE
Department of Morphological Sciences, School of Medicine, University of Granada, Spain.
Melguizo
C
C
Prados
J
J
Marchal
J A
JA
Alvarez
L
L
Aránega
A
A
D000199:Actins / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D002642:Chick Embryo; D003429:Cross Reactions; D006321:Heart / Q000196:embryology; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D011817:Rabbits; D012518:Sarcomeres / Q000378:metabolism*; D014018:Tissue Distribution
D000199:Actins; D000911:Antibodies, Monoclonal