title: Investigation of an epidemic of invasive aspergillosis: utility of molecular typing with the use of random amplified polymorphic DNA probes.
abstract: When seven immunocompromised patients developed invasive aspergillosis during construction at a hospital, new methods were performed to compare fungal isolates and a case-control study was conducted to determine risks for infection. Typing of Aspergillus flavus with the use of restriction endonuclease analysis and restriction fragment length polymorphism using random amplified polymorphic DNA reactions to generate DNA probes revealed different patterns between isolates from two patients and a similar pattern among those from one patient, a health care worker, and an environmental source. Case patients were more likely than controls to have longer periods of hospitalization (median, 83 vs. 24 days; P < 0.01), neutropenia (median, 33 vs. 6 days; P < 0.05), and exposure to broad spectrum antimicrobials (median, 56 vs. 15 days; P = 0.08). No patients restricted to protected areas developed aspergillosis. Risk of exposure of immunocompromised patients to opportunistic organisms stirred up by construction activity may be decreased by admitting these patients to protected areas away from construction activity and by restricting traffic from construction sites to these areas. Although typing of A. flavus isolates did not reveal a single type or source of organism responsible for infection, this method may facilitate epidemiologic investigation of possible nosocomial sources and transmission in similar settings.
authors.affiliation: Epidemic Intelligence Service, Centers for Disease Control and Prevention, Atlanta, GA 30333.
authors.forename: J
authors.initials: J
authors.lastname: Buffington
authors.forename: R
authors.initials: R
authors.lastname: Reporter
authors.forename: B A
authors.initials: BA
authors.lastname: Lasker
authors.forename: M M
authors.initials: MM
authors.lastname: McNeil
authors.forename: J M
authors.initials: JM
authors.lastname: Lanson
authors.forename: L A
authors.initials: LA
authors.lastname: Ross
authors.forename: L
authors.initials: L
authors.lastname: Mascola
authors.forename: W R
authors.initials: WR
authors.lastname: Jarvis
mesh_terms: D000293:Adolescent; D000328:Adult; D001228:Aspergillosis / Q000453:epidemiology / Q000209:etiology*; D001230:Aspergillus / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D015342:DNA Probes; D005784:Gene Amplification; D006801:Humans; D007231:Infant, Newborn; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors
chemical_list: D015342:DNA Probes