Induction, transcription, synthesis, and adsorption of interleukin-1 by dialyzer membranes.
This study was designed to dissect the direct effect of dialyzer membrane on interleukin-1 (IL-1) induction from those of complement activation, mechanical stimulation, acetate/bicarbonate and endotoxin diffusion, and cell type interactions. To this end, a suspension of P388D1 murine macrophages in a complement-free culture medium containing 10% heat-inactivated serum, a closed-loop system consisting of tubing alone or with a hollow-fiber cuprammonium cellulose (CU) or polyacrylonitrile (PAN) dialyzer, and a roller pump were used. The dialysate compartment was filled with the same medium and capped. Cell suspension was recirculated at 300 mL/min for 3 h. Cells and supernates were separated, and adhering proteins were eluted. All samples tested negative for endotoxin. IL-1 mRNA was greatest with CU, followed by PAN and tubing alone. IL-1 in the supernate was greater with CU than with either tubing alone or PAN (P < 0.005; analysis of variance), which showed comparable values. IL-1 eluted from loops was greatest with PAN dialyzers, followed by sets with CU dialyzers and tubing alone (P < 0.001; analysis of variance). Thus, both CU and PAN membranes directly induce IL-1. However, avid adsorption by PAN attenuates the rise in circulating IL-1.
Vaziri
N D
ND
Department of Medicine, University of California, Irvine.
Wang
J
J
Cesario
T
T
Yousefi
S
S
Valenzuela
R
R
Carandang
G
G
D000180:Acrylic Resins / Q000009:adverse effects; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D002460:Cell Line; D002482:Cellulose / Q000009:adverse effects / Q000031:analogs & derivatives; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics / Q000493:pharmacokinetics; D007688:Kidneys, Artificial / Q000009:adverse effects; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D006435:Renal Dialysis / Q000009:adverse effects; D014158:Transcription, Genetic
D000180:Acrylic Resins; D001672:Biocompatible Materials; D007375:Interleukin-1; D008567:Membranes, Artificial; D012333:RNA, Messenger; C010504:polyacrylonitrile; D002482:Cellulose; C009865:cuprammonium cellulose