Anti-complement activity of oleanolic acid: an inhibitor of C3-convertase of the classical complement pathway.
Oleanolic acid is a naturally occurring compound, isolated from Luffa cylindrica, which inhibits the in-vitro immunohaemolysis of antibody-coated sheep erythrocytes by guinea-pig serum. In further experiments this reduced immunohaemolysis was found to be due to inhibition of the C3-convertase of the classical complement pathway. The threshold concentration for inhibition of C3-convertase was 100 micrograms mL-1. However, higher concentrations of oleanolic acid showed constant inhibitory effects on immunohaemolysis. Oleanolic acid also exhibited weak inhibitory effects on individual components of the complement system.
Kapil
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Pharmacology Division, Regional Research Laboratory, Jammu Tawi, India.
Sharma
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D000818:Animals; D003176:Complement C3 / Q000737:chemistry / Q000302:isolation & purification; D050577:Complement C3-C5 Convertases / Q000037:antagonists & inhibitors*; D003169:Complement Inactivator Proteins / Q000494:pharmacology*; D003171:Complement Pathway, Classical / Q000187:drug effects*; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D006461:Hemolysis / Q000187:drug effects; D007052:Ibuprofen / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009828:Oleanolic Acid / Q000494:pharmacology*; D012756:Sheep / Q000276:immunology
D003176:Complement C3; D003169:Complement Inactivator Proteins; D009828:Oleanolic Acid; D050577:Complement C3-C5 Convertases; D007052:Ibuprofen