Molecular cloning and tissue expression of human mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase.
Mitochondrial 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase is a constituent of the HMG-CoA pathway responsible for ketone body synthesis and one of its main regulatory points. We report the isolation and characterization of a 2058-bp cDNA from human liver. This cDNA encodes a polypeptide of 508 residues and 56,635 Da. The homology with previously reported rat mitochondrial and human cytosolic HMG-CoA synthases is 88 and 66%, respectively. mRNA levels were high in liver and colon, low in testis, heart, skeletal muscle, and kidney, and faint in pancreas.
Mascaró
C
C
Unit of Biochemistry, School of Pharmacy, University of Barcelona, Spain.
Buesa
C
C
Ortiz
J A
JA
Haro
D
D
Hegardt
F G
FG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003106:Colon / Q000201:enzymology; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D015870:Gene Expression; D006801:Humans; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000235:genetics*; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D051381:Rats; D015183:Restriction Mapping
D018076:DNA, Complementary; D006904:Hydroxymethylglutaryl-CoA Synthase