title: Clinical evaluation of biotin-binding immunoglobulin in patients with Graves' disease.
abstract: Biotin-binding immunoglobulin (BBI) was recently identified in human serum and has been suggested to have a significant association with allergic and autoimmune disorders. Attempts were made to evaluate the clinical significance of BBI in autoimmune thyroid disorders. Prevalence of BBI was significantly higher in Graves' disease (47%) than in Hashimoto's disease (8%) and healthy controls (10%). The BBI consisted of heterogeneous subtypes with respect to binding of several immunoglobulin classes. Sera in Graves' disease showed predominantly IgG-binding BBI, whereas healthy subjects had IgM-binding BBI. Thyroid stimulating hormone receptor antibody (TRAb) level was significantly higher in the BBI non-detected group than in the detected group. There was no significant relationship between BBI prevalence and thyroid hormone concentrations, anti-thyroglobulin antibody (TGAb) or anti-thyroid microsomal antibody (McAb) titers. In addition, biotin levels in peripheral blood and red blood cells and biotinidase activity did not differ in the BBI detected and non-detected groups. The present results suggest that BBI is associated with autoimmune dysfunction in Graves' disease.
authors.affiliation: First Department of Internal Medicine, Gunma University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nagamine
authors.forename: K
authors.initials: K
authors.lastname: Takehara
authors.forename: T
authors.initials: T
authors.lastname: Fukui
authors.forename: M
authors.initials: M
authors.lastname: Mori
mesh_terms: D000328:Adult; D000581:Amidohydrolases / Q000097:blood; D001323:Autoantibodies / Q000097:blood; D001667:Binding, Competitive; D001710:Biotin / Q000097:blood; D043523:Biotinidase; D002352:Carrier Proteins / Q000097:blood*; D005260:Female; D006111:Graves Disease / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000097:blood*; D018828:Immunoglobulins, Thyroid-Stimulating; D008297:Male; D008875:Middle Aged; D013967:Thyroiditis, Autoimmune / Q000276:immunology; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
chemical_list: D001323:Autoantibodies; D002352:Carrier Proteins; D007136:Immunoglobulins; D018828:Immunoglobulins, Thyroid-Stimulating; C017071:biotin-binding proteins; D014284:Triiodothyronine; D001710:Biotin; D013972:Thyrotropin; D000581:Amidohydrolases; D043523:Biotinidase; D013974:Thyroxine