Unique T cell differentiation markers: gangliosides with cholera toxin receptor activity on murine fetal thymocytes.
Cholera toxin B subunit receptors (CTBR; gangliosides GM1a and GM1b-GalNAc-Gal) and GM1b-type gangliosides were examined during T cell development in BALB/c mice by FACS or TLC immunostaining. Sixty-two percent of CD4-8- early fetal thymocytes express CTBR [58% low affinity (GM1a+), 4% high affinity (GM1a2+)] at 13 days gestation (Day 13); GM1a2+ was expressed preferentially on Thy-1+ fetal thymocytes after Day 13. GM1a2+ Thy-1+ cells increased from 4% on Day 13 to 95% on Day 17. Surface GM1a2+ decreased beginning on Day 15 while GM1b-GalNAc-Gal (and CD4 and CD8) increased. GM1b-type+ cells increased from 8% on Day 13 to 51% on Day 17 and then decreased to 16% at 4 weeks; few asialo GM1+ cells (< 5%) are seen during gestation. Thus, synthesis of GM1a- and GM1b-type series was active until Days 15 and 17, respectively, and then was suppressed; GM1b-GalNAc-Gal was synthesized from GM1b following Gestational Day 15. GM1a- and GM1b-type identify immature T cells in mice, while GM1b-GalNAc-Gal identifies mature T cells.
Noguchi
M
M
Department of Rheumatology, Juntendo University School of Medicine, Tokyo, Japan.
Suping
Z
Z
Taguchi
J
J
Hirano
T
T
Hashimoto
H
H
Hirose
S
S
Iwamori
M
M
Okumura
K
K
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry; D000954:Antigens, Surface / Q000378:metabolism; D002240:Carbohydrate Sequence; D002772:Cholera Toxin / Q000378:metabolism; D005333:Fetus / Q000276:immunology; D005677:G(M1) Ganglioside / Q000737:chemistry / Q000276:immunology*; D005732:Gangliosides / Q000737:chemistry / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018800:Thy-1 Antigens
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D005732:Gangliosides; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D018800:Thy-1 Antigens; C023552:choleragen receptor; D005677:G(M1) Ganglioside; D002772:Cholera Toxin