title: [Design of a chromosomal DNA probe species specific for Yersinia pestis].
abstract: A 14.8 kb DNA fragment from the chromosome of Yersinia pestis TWJ was cloned and the restriction map constructed. The fragment designated as T16 and its subfragments were tested in dot-hybridization with strains of Yersinia genus and other members of Enterobacteriaceae. A species-specific DNA probe (designated MK) was constructed on the basis of the T16 fragment. As judged from restriction analysis, blot-hybridization experiments and, partially, sequencing, significant homology exists between the MK DNA probe and this one developed by Bardarov et. al. (1990). A repeated sequence in two copies was discovered in the MK fragment.
authors.forename: O V
authors.initials: OV
authors.lastname: Norkina
authors.forename: A N
authors.initials: AN
authors.lastname: Kulichenko
authors.forename: G A
authors.initials: GA
authors.lastname: Shovadaeva
authors.forename: R Kh
authors.initials: RKh
authors.lastname: Boshnakov
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Aksenov
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Popov
authors.forename: A Ts
authors.initials: ATs
authors.lastname: Tomov
authors.forename: V K
authors.initials: VK
authors.lastname: K''esovskiĭ
authors.forename: I G
authors.initials: IG
authors.lastname: Drozdov
authors.forename: A L
authors.initials: AL
authors.lastname: Gintsburg
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D013045:Species Specificity; D015010:Yersinia pestis / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial
vernacular_title: Konstruirovanie vidospetsifichnogo dlia Yersinia pestis khromosomnogo DNA-zonda.