Transcriptional regulation of estrogen receptor in breast carcinomas.
An important transcriptional regulatory element of the estrogen receptor (ER) gene that binds a protein expressed in ER-positive breast carcinomas has been identified. Using a transient expression assay, we identified a 75-bp region of the 5' untranslated leader of the ER gene which augments expression from the ER promoter. This region contains two binding sites for a protein, estrogen receptor factor 1 (ERF-1), which is expressed in ER-positive breast carcinomas. A concatenated ERF-1 binding site probe identified a 30,000-Da protein. Low-level ERF-1 expression was detected in normal human mammary epithelial cells. Abundant ERF-1 expression was also found in endometrial carcinoma cell lines that express the ER-positive phenotype. These results indicate that ERF-1 expression represents a common mechanism of ER regulation in hormonally responsive carcinomas.
deConinck
E C
EC
Department of Surgery, Stanford University, California.
McPherson
L A
LA
Weigel
R J
RJ
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics*; D004252:DNA Mutational Analysis; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000096:biosynthesis / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic
D015342:DNA Probes; D004268:DNA-Binding Proteins; D011960:Receptors, Estrogen; D014157:Transcription Factors