title: Conjugation types of 7 beta-hydroxylated bile acids detected in healthy human urine.
abstract: Bile acids extracted from the urine of a healthy volunteer who excreted 7 beta-hydroxylated bile acids were fractionated to nonamidated, glycine-conjugated, taurine-conjugated, and sulfated bile acid fractions. The chemical conjugation types of the 7 beta-hydroxylated bile acids were then determined by treatment with several enzymes and by capillary column gas chromatography. Large amounts of 3 alpha,7 beta,12 alpha-trihydroxycholanoic acid were present as nonamidated and nonconjugated bile acids, while 3 beta,7 beta-dihydroxycholanoic acid formed nonamidated bile acid N-acetylglucosaminide. In addition, ursodeoxycholic acid formed both glycine-conjugated bile acid and glycine-conjugated bile acid N-acetylglucosaminide. Bile acid N-acetylglucosaminides were hydrolyzed by solvolysis.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Tottori University.
authors.forename: N
authors.initials: N
authors.lastname: Yamaga
authors.forename: H
authors.initials: H
authors.lastname: Kohara
mesh_terms: D001647:Bile Acids and Salts / Q000737:chemistry* / Q000378:metabolism / Q000652:urine*; D019826:Cholic Acid; D002793:Cholic Acids / Q000737:chemistry* / Q000652:urine; D002849:Chromatography, Gas; D006801:Humans; D014580:Ursodeoxycholic Acid / Q000737:chemistry* / Q000652:urine
chemical_list: D001647:Bile Acids and Salts; D002793:Cholic Acids; D014580:Ursodeoxycholic Acid; D019826:Cholic Acid