Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum).
An Aspergillus niger (ficuum) genomic DNA lambda EMBL3 library was probed with a 354-bp DNA fragment obtained by polymerase chain reaction of A. niger DNA with oligonucleotides based on partial amino acid sequence of a pH 2.5 optimum acid phosphatase. A clone containing a 1605 bp segment (phyB) encoding the 479 amino acid enzyme was isolated and found to contain four exons. Global alignment revealed 23.5% homology to Aspergillus niger phytase (PhyA); four regions of extensive homology were identified. Some of these regions may contain catalytic sites for phosphatase function.
Ehrlich
K C
KC
Southern Regional Research Center, United States Department of Agriculture, New Orleans, LA 70179.
Montalbano
B G
BG
Mullaney
E J
EJ
Dischinger
H C
HC
Ullah
A H
AH
D010832:6-Phytase / Q000235:genetics*; D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005800:Genes, Fungal; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D017386:Sequence Homology, Amino Acid; D012984:Software
D004271:DNA, Fungal; D007527:Isoenzymes; D009838:Oligodeoxyribonucleotides; D010832:6-Phytase