title: Differential localization of two isoforms of the regulatory subunit RII alpha of cAMP-dependent protein kinase in human sperm: biochemical and cytochemical study.
abstract: In the present study, immunogold labeling of ultrathin sections of ejaculated sperm was used to obtain insight into the ultrastructural localization and presumable function of type II cAMP-dependent protein kinase in sperm motion. In the flagellum, a human-specific isoform of the RII alpha subunit was located on the axonemal microtubule wall, whereas a different isoform of broader specificity was present in the cytoplasm at the periphery of the coarse fibers and fibrous sheath. This isoform was also found in the mitochondria. The human-specific RII alpha subunit is likely linked to microtubules by a unique binding protein of M(r) 72 kD. These findings are in agreement with the concept of a concerted mechanism involving phosphorylation of both the axonemal microtubules and the fibrous structures for the regulation of mammalian sperm motion.
authors.affiliation: Département de Biochimie, UFR Biomédicale des Saints-Pères, Université René Descartes, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Pariset
authors.forename: S
authors.initials: S
authors.lastname: Weinman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D015153:Blotting, Western; D002417:Cattle; D054754:Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000032:analysis*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D046911:Macromolecular Substances; D008297:Male; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D013077:Sperm Head / Q000201:enzymology / Q000648:ultrastructure; D013082:Sperm Tail / Q000201:enzymology / Q000648:ultrastructure; D013094:Spermatozoa / Q000201:enzymology* / Q000648:ultrastructure; D013737:Testis / Q000201:enzymology
chemical_list: D000906:Antibodies; D054754:Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit; D007527:Isoenzymes; D046911:Macromolecular Substances; C517819:PRKAR2A protein, human; D010455:Peptides; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases