title: Effect of fat feeding on human intestinal apolipoprotein B mRNA levels and editing.
abstract: Our purpose was to assess the effect of a fat-rich meal on intestinal apolipoprotein B (apoB) mRNA levels and editing. We obtained jejunal biopsies from eight healthy adults in the fasting state and 3 h after a meal containing 1 g/kg of fat. In the fasting state, 93% of the apoB mRNA contained the editing sequence resulting in apoB-48 production. Feeding induced no significant changes in apoB mRNA levels or editing. Our data are consistent with the concept that the significant increase in apoB-48 within triglyceride-rich lipoproteins in postprandial plasma is not due to alterations in apoB gene expression or editing.
authors.affiliation: Lipid Metabolism Laboratory, Tufts University, Boston, MA 02111.
authors.forename: J
authors.initials: J
authors.lastname: Lopez-Miranda
authors.forename: N
authors.initials: N
authors.lastname: Kam
authors.forename: J
authors.initials: J
authors.lastname: Osada
authors.forename: C
authors.initials: C
authors.lastname: Rodriguez
authors.forename: P
authors.initials: P
authors.lastname: Fernandez
authors.forename: J
authors.initials: J
authors.lastname: Contois
authors.forename: E J
authors.initials: EJ
authors.lastname: Schaefer
authors.forename: J M
authors.initials: JM
authors.lastname: Ordovas
mesh_terms: D001055:Apolipoproteins B / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D004041:Dietary Fats / Q000494:pharmacology*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008969:Molecular Sequence Data; D017393:RNA Editing; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D001055:Apolipoproteins B; D004041:Dietary Fats; D008055:Lipids; D012333:RNA, Messenger