title: The role of Igh-1 disparate congenic mouse T lymphocytes in the pathogenesis of herpetic stromal keratitis.
abstract: The corneal destruction associated with herpes simplex keratitis (HSK) is primarily the result of the host's immune response to herpes simplex virus type-1 (HSV-1) infection. We examined the role of T cells and T cell subsets in the pathogenesis of HSK. Naive and immune T cells and HSV-1 immune CD4+ and CD8+ subsets from Igh-1 disparate BALB/c congenic mice were adoptively transferred into athymic BALB/c nude mice, which normally do not develop HSK. The results demonstrated that while the transfer of naive T cells from either HSK-susceptible C.AL-20 (Igh-1d) or HSK-resistant C.B-17 (Igh-1b) mice had little influence on HSK development, transfer of either CD3+ or CD4+ HSV-1 immune T cells from C.AL-20 mice resulted in the development of severe HSK in all of the recipients. Transfer of the same cell populations from C.B-17 mice resulted in the development of only a mild keratitis in 50% of the recipients. Transfer of CD8+ cells from either donor strain did not result in stromal disease in any recipient mouse. These results clearly demonstrate the pivotal role of CD4+ T cells in the development of necrotizing herpes stromal keratitis, and further demonstrate that CD8+ T cells are not essential in HSK development in the BALB/c system.
authors.affiliation: Hilles Immunology Laboratory, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston 02114.
authors.forename: Y A
authors.initials: YA
authors.lastname: Akova
authors.forename: J
authors.initials: J
authors.lastname: Dutt
authors.forename: A
authors.initials: A
authors.lastname: Rodriguez
authors.forename: N
authors.initials: N
authors.lastname: Jabbur
authors.forename: C S
authors.initials: CS
authors.lastname: Foster
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology; D003319:Corneal Stroma / Q000382:microbiology; D005434:Flow Cytometry; D018259:Herpesvirus 1, Human / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D016130:Immunophenotyping; D016219:Immunotherapy, Adoptive; D015994:Incidence; D016849:Keratitis, Herpetic / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D016827:CD8 Antigens; D007143:Immunoglobulin Heavy Chains