title: Hybrid scanning transmission electron/scanning tunneling microscope system for the preparation and investigation of biomolecules.
abstract: A hybrid scanning transmission electron/scanning tunneling microscope vacuum system is introduced, which allows freeze drying and metal coating of biological samples and their simultaneous observation by scanning transmission electron microscopy and scanning tunnelling microscopy (STM). Different metal coatings and STM tips were analysed to obtain the highest possible resolution for such a system. Bovine liver catalase was used as a test sample and the STM results are compared to a molecular scale model.
authors.affiliation: Maurice E. Müller Institute for Microscopic Structural Biology, Biozentrum, University of Basel, Switzerland.
authors.forename: H F
authors.initials: HF
authors.lastname: Knapp
authors.forename: R
authors.initials: R
authors.lastname: Wyss
authors.forename: R
authors.initials: R
authors.lastname: Häring
authors.forename: C
authors.initials: C
authors.lastname: Henn
authors.forename: R
authors.initials: R
authors.lastname: Guckenberger
authors.forename: A
authors.initials: A
authors.lastname: Engel
mesh_terms: D000818:Animals; D017122:Bacteriophage T4 / Q000648:ultrastructure; D002213:Capsid / Q000648:ultrastructure; D002374:Catalase / Q000648:ultrastructure*; D002417:Cattle; D005612:Freeze Drying; D008099:Liver / Q000201:enzymology; D017348:Microscopy, Electron, Scanning Transmission / Q000295:instrumentation / Q000379:methods*; D016252:Microscopy, Scanning Tunneling / Q000295:instrumentation / Q000379:methods*; D013048:Specimen Handling / Q000379:methods
chemical_list: D002374:Catalase