title: Dithranol (anthralin) and 10-butyryl dithranol (butantrone) do not morphologically transform cultured C3H 10T1/2 C18 mouse embryo fibroblasts.
abstract: The ability of dithranol and 10-butyryl dithranol to induce morphological cell transformation was studied in cultured C3H 10T1/2 C18 fibroblasts. The cells were incubated with different concentrations of the test compounds for 48 h and cultured for 5 weeks thereafter. At the end of the culture period the cultures were fixed, stained and examined for the presence of transformed foci. Dithranol and 10-butyryl dithranol did not increase the formation of transformed foci, while the positive control compound, 7,12-dimethylbenz(a)anthracene (DMBA), induced a high frequency of transformations significantly different from controls. Thus the in vitro cell transformation model with uninitiated C3H 10T1/2 C18 fibroblasts is not able to detect the weak tumorigenic action of dithranol and 10-butyryl dithranol which has been observed in mouse skin.
authors.affiliation: Department of Pharmacology and Toxicology, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Viluksela
authors.forename: F M
authors.initials: FM
authors.lastname: Debets
authors.forename: J C
authors.initials: JC
authors.lastname: van der Hoeven
authors.forename: P T
authors.initials: PT
authors.lastname: Männistö
mesh_terms: D000818:Animals; D000875:Anthralin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000187:drug effects / Q000473:pathology*; D005347:Fibroblasts / Q000187:drug effects*; D007509:Irritants / Q000494:pharmacology*; D051379:Mice
chemical_list: D007509:Irritants; C038464:butantrone; D000875:Anthralin