B-lymphoid to granulocytic switch during hematopoiesis in a transgenic mouse strain.
B-lymphoid and myeloid development are markedly perturbed in a unique transgenic mouse strain, max 41. Pro-B, pre-B, and B lymphocytes were severely reduced but granulocytes were greatly elevated. This phenotype could be adoptively transferred with bone marrow cells. It was not alleviated by bcl-2 or myc transgenes that promote lymphocyte survival or proliferation. Bitransgenic myc/max 41 mice developed pre-B cell lymphoma. An accompanying massive granulocytosis unexpectedly proved to be clonally derived from the pre-B lymphoma cells. These observations suggest that B lymphopoiesis in max 41 mice has been diverted to granulocyte production. Since neither cell type expressed the transgene, this novel lymphomyeloid deviation probably reflects insertional alteration of a hematopoietic regulatory gene.
Lindeman
G J
GJ
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Melbourne, Australia.
Adams
J M
JM
Cory
S
S
Harris
A W
AW
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D015152:Blotting, Northern; D015139:Blotting, Southern; D016026:Bone Marrow Transplantation / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D005314:Embryonic and Fetal Development; D005434:Flow Cytometry; D006098:Granulocytes / Q000502:physiology*; D006411:Hematopoiesis, Extramedullary / Q000235:genetics* / Q000502:physiology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic