title: Purification of a low molecular weight microtubule binding protein from sea urchin eggs.
abstract: A low molecular weight microtubule binding protein(SU-MAP34) was purified from sea urchin eggs. This protein bound strongly to the microtubule formed from purified echinoderm tubulin but showed no cross-linking of microtubules. Monospecific antibody against SU-MAP34 was produced and an immunoblotting analysis showed that this protein was not a breakdown product of a protein of a higher molecular mass. Whole cell staining and confocal laser scanning microscope observation showed that SU-MAP34 localized on the filamentous structure of mitotic apparatus and this structure was identified as the microtubule with double staining using anti-SU-MAP34 and anti-tubulin. An immunoblotting experiment showed an enrichment of SU-MAP34 in a microtubule protein fraction prepared using taxol from a crude extract of the cell.
authors.affiliation: Graduate School, Division of Science, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Maekawa
authors.forename: M
authors.initials: M
authors.lastname: Mishima
authors.forename: M
authors.initials: M
authors.lastname: Toriyama
authors.forename: H
authors.initials: H
authors.lastname: Sakai
mesh_terms: D000818:Animals; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D010063:Ovum / Q000737:chemistry*; D017239:Paclitaxel / Q000494:pharmacology; D012617:Sea Urchins; D014404:Tubulin / Q000378:metabolism
chemical_list: D008869:Microtubule-Associated Proteins; D014404:Tubulin; D017239:Paclitaxel