Decreased CD4+CD45RA+ lymphocytes in peripheral blood of systemic lupus erythematosus can recover after separation from patient's sera.
Decrease in CD4+CD45RA+ lymphocytes in peripheral blood of systemic lupus erythematosus (SLE) patients has been reported. In this study, mononuclear cells were separated from peripheral blood of SLE patients, and the CD4+CD45RA+ cells were counted by flow cytometry just after separation and also 1 week after incubation in vitro. Furthermore, healthy lymphocytes were incubated with SLE patient's sera, and the Ca2+ level was measured to investigate the interaction of patient's sera with healthy lymphocytes. The CD4+CD45RA+ lymphocytes were found to be decreased in number on the first day just after separation of lymphocytes, but recovered to normal levels after 1 week culture without patient's sera. The intracellular Ca2+ level in normal lymphocytes increased 1 min after incubation with patient's sera, but not with healthy control sera. These results suggest that CD4+CD45RA+ cells are persistently activated in the peripheral blood of SLE patients, and that their sera contain some extrinsic factors which could activate the lymphocytes.
Nobutoh
T
T
Department of Dermatology, Kawasaki Medical School, Okayama, Japan.
Kohda
M
M
Ueki
H
H
D000328:Adult; D000368:Aged; D015704:CD4 Antigens / Q000032:analysis*; D002118:Calcium / Q000378:metabolism; D005260:Female; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000032:analysis*; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D013997:Time Factors
D015704:CD4 Antigens; D017493:Leukocyte Common Antigens; D002118:Calcium