title: Tumour necrosis factor-alpha induces an increase in susceptibility of human glioblastoma U87-MG cells to natural killer cell-mediated lysis.
abstract: The mechanism by which tumour necrosis factor (TNF)-alpha increases the susceptibility of U87-MG human glioblastoma cells to lysis by natural killer (NK) cells was studied. Treatment with TNF-alpha (100 units ml-1) for 48 h enhanced the susceptibility of tumour cells to lysis by NK cells. Increased susceptibility to lysis was associated with enhanced expression of intercellular adhesion molecule 1 (ICAM-1) and HLA class I antigen. Antisense ICAM-1 oligonucleotide inhibited lysis by NK cells of TNF-alpha-treated tumour cells. In contrast, acid treatment following TNF-alpha treatment increased lysis by NK cells. These findings indicate that TNF-alpha treatment of glioblastoma cells increased their susceptibility to lysis by NK cells, since ICAM-1 up-regulation would have more profound effects on NK susceptibility than would HLA class I antigen up-regulation.
authors.affiliation: Department of Neurosurgery, Utano National Hospital, Kyoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kondo
authors.forename: D
authors.initials: D
authors.lastname: Yin
authors.forename: J
authors.initials: J
authors.lastname: Takeuchi
authors.forename: T
authors.initials: T
authors.lastname: Morimura
authors.forename: S I
authors.initials: SI
authors.lastname: Miyatake
authors.forename: S
authors.initials: S
authors.lastname: Nakatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Oda
authors.forename: H
authors.initials: H
authors.lastname: Kikuchi
mesh_terms: D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000276:immunology; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005455:Fluorescent Antibody Technique; D005909:Glioblastoma / Q000276:immunology* / Q000473:pathology; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000276:immunology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007694:Killer Cells, Natural / Q000502:physiology*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D015395:Histocompatibility Antigens Class I; D016376:Oligonucleotides, Antisense; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1