title: Involvement of CD11b/CD18 in enhanced neutrophil adhesion by Fc gamma receptor stimulation.
abstract: Neutrophils showed a rapid and transient adhesion to immunoglobulin G (IgG)-coated plates compared with their adhesion to bovine serum albumin (BSA)-coated plates: the adhesion reached a peak after 15 min of incubation and then gradually returned to almost the basal state in 60 min. The addition of monomeric IgG or anti-Fc gamma RII monoclonal antibody (mAb) (IV.3) suppressed the increase in adhesion, whereas anti-Fc gamma RIII mAb (3G8) was hardly effective, indicating that the interaction of Fc gamma R, especially Fc gamma RII, with coated IgG is involved in the process. Adhesion was also blocked by cytochalasin B, suggesting that functional actin filament structures are crucial. Protein kinase inhibitors, erbstatin and genistein, inhibited the adhesion in a dose-dependent manner. The adhesion was inhibited by anti-CD11b (M1/70) and anti-CD18 (MHM23, TS1/18) mAbs. Moreover, neutrophils from a patient with complete leukocyte adhesion deficiency syndrome did not show increased adhesion to IgG-coated plates. The adhesion of neutrophils to fibrinogen- and BSA-coated plates was also increased when Fc gamma R was stimulated in the fluid phase with soluble aggregated IgG, which was also inhibited by anti-CD11b mAb. Stimulation of neutrophil Fc gamma R with soluble aggregated IgG enhanced the expression of CD11b in concert with the enhanced adhesion. These data collectively suggest that stimulation via Fc gamma R evokes a tyrosine kinase-dependent and actin filament-dependent intracellular signal that enhances the specific and nonspecific adhesive activity of neutrophils, presumably through the activation of CD11b/CD18.
authors.affiliation: Division of Pediatrics, South Kyoto National Hospital, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kusunoki
authors.forename: S
authors.initials: S
authors.lastname: Tsuruta
authors.forename: H
authors.initials: H
authors.lastname: Higashi
authors.forename: S
authors.initials: S
authors.lastname: Hosoi
authors.forename: D
authors.initials: D
authors.lastname: Hata
authors.forename: K
authors.initials: K
authors.lastname: Sugie
authors.forename: M
authors.initials: M
authors.lastname: Mayumi
authors.forename: H
authors.initials: H
authors.lastname: Mikawa
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000502:physiology*; D018845:CD11 Antigens; D018821:CD18 Antigens; D003571:Cytochalasin B / Q000494:pharmacology; D019833:Genistein; D006801:Humans; D006873:Hydroquinones / Q000494:pharmacology; D007074:Immunoglobulin G / Q000494:pharmacology; D066298:In Vitro Techniques; D007529:Isoflavones / Q000494:pharmacology; D007700:Kinetics; D051379:Mice / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism; D017452:Receptors, IgG / Q000276:immunology / Q000502:physiology*; D016028:Receptors, Leukocyte-Adhesion / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; D006873:Hydroquinones; D007074:Immunoglobulin G; D007529:Isoflavones; D047428:Protein Kinase Inhibitors; D017452:Receptors, IgG; D016028:Receptors, Leukocyte-Adhesion; D003571:Cytochalasin B; D019833:Genistein; D011494:Protein Kinases; C048212:erbstatin