title: Glycosylation and phosphorylation of arylsulfatase A.
abstract: The glycosylation and phosphorylation of the lysosomal enzyme arylsulfatase A was analyzed by a combination of metabolic labeling, tryptic fragmentation, mass spectrometry, and radiosequencing. The results demonstrate that all three potential N-glycosylation sites at Asn residues 158, 184, and 350 are utilized in arylsufatase A and carry high mannose or hybride type oligosaccharides. Phosphorylation of mannose residues is restricted to oligosaccharides at the first and third N-glycosylation site (Asn-158 and Asn-350). Both are phosphorylated with comparable efficiency. An earlier study had shown that a mutant arylsulfatase A containing only the second N-glycosylation site at Asn-184 folds correctly and is phosphorylated (Gieselmann, V., Schmidt, B., and von Figura, K. (1992) J. Biol. Chem. 267, 13262-13266). The lack of phosphorylation at Asn-184 in wild type arylsulfatase A therefore indicates that in vivo the presence of oligosaccharides can interfere with phosphorylation of other sites or that phosphorylation occurs in an ordered manner whereby phosphorylation of one site can affect the phosphorylation of another site.
authors.affiliation: Georg-August-Universität, Zentrum für Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, Göttingen, Federal Republic of Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Sommerlade
authors.forename: T
authors.initials: T
authors.lastname: Selmer
authors.forename: A
authors.initials: A
authors.lastname: Ingendoh
authors.forename: V
authors.initials: V
authors.lastname: Gieselmann
authors.forename: K
authors.initials: K
authors.lastname: von Figura
authors.forename: K
authors.initials: K
authors.lastname: Neifer
authors.forename: B
authors.initials: B
authors.lastname: Schmidt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002553:Cerebroside-Sulfatase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D006031:Glycosylation; D006801:Humans; D006868:Hydrolysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010766:Phosphorylation; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002553:Cerebroside-Sulfatase