[Expression of proliferating cell nuclear antigen following filtration surgery in rabbits].
We evaluated cell replication in wound healing following experimental filtration surgery. Cell replication was measured via immunohistochemistry for proliferating cell nuclear antigen, which is a marker of cell proliferation. Trabeculectomy was performed on albino rabbit eyes, with the filtering site harvested on postoperative days 1, 4, 7, and 14. Samples were fixed with 10% buffered formalin and prepared for paraffin section, and proliferating cell nuclear antigen was detected in filtering site tissue using the labeled streptavidin-biotin method. The number of immunoreactive cells increased at days 4 to 7 and was markedly decreased at day 14; the filtering site was obstructed at days 7 to 14. These findings may have clinical implications for the administration of antiproliferative agents after filtration surgery. Evaluation of cell replication following filtration surgery by detecting proliferating cell nuclear antigen is a simpler technique than autoradiography.
Watanabe
J
J
Department of Ophthalmology, School of Medicine, Niigata University, Japan.
Sawaguchi
S
S
Abe
H
H
Iwata
K
K
D000818:Animals; D002455:Cell Division; D005123:Eye / Q000166:cytology / Q000378:metabolism*; D005374:Filtration; D007150:Immunohistochemistry; D009687:Nuclear Proteins / Q000378:metabolism*; D013508:Ophthalmologic Surgical Procedures; D018809:Proliferating Cell Nuclear Antigen; D011817:Rabbits; D014130:Trabeculectomy; D014945:Wound Healing / Q000502:physiology
D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen