title: Chemical studies of phytoestrogens and related compounds in dietary supplements: flax and chaparral.
abstract: High-performance liquid chromatographic (HPLC) and mass spectrometric (MS) procedures were developed to determine lignans in flaxseed (Linum usitatissimum) and chaparral (Larrea tridentata). Flaxseed contains high levels of phytoestrogens. Chaparral has been associated with acute nonviral toxic hepatitis and contains lignans that are structurally similar to known estrogenic compounds. Both flaxseed and chaparral products have been marketed as dietary supplements. A mild enzyme hydrolysis procedure to prevent the formation of artifacts in the isolation step was used in the determination of secoisolariciresinol in flaxseed products. HPLC with ultraviolet spectral (UV) or MS detection was used as the determinative steps. HPLC procedures with UV detection and mass spectrometry were developed to characterize the phenolic components, including lignans and flavonoids, of chaparral and to direct fractionation studies for the bioassays.
authors.affiliation: Division of Natural Products, Food and Drug Administration, Washington, District of Columbia 20204.
authors.forename: W R
authors.initials: WR
authors.lastname: Obermeyer
authors.forename: S M
authors.initials: SM
authors.lastname: Musser
authors.forename: J M
authors.initials: JM
authors.lastname: Betz
authors.forename: R E
authors.initials: RE
authors.lastname: Casey
authors.forename: A E
authors.initials: AE
authors.lastname: Pohland
authors.forename: S W
authors.initials: SW
authors.lastname: Page
mesh_terms: D002072:Butylene Glycols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D005527:Food, Fortified / Q000032:analysis*; D007529:Isoflavones; D017705:Lignans / Q000032:analysis*; D008031:Lignin / Q000032:analysis; D013058:Mass Spectrometry; D048789:Phytoestrogens; D028321:Plant Preparations; D010944:Plants / Q000737:chemistry*
chemical_list: D002072:Butylene Glycols; D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; D017705:Lignans; D048789:Phytoestrogens; D028321:Plant Preparations; D008031:Lignin; C060283:secoisolariciresinol