HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.
The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.
Olsen
J
J
Department of Medical Biochemistry, Panum Institute, University of Copenhagen, Denmark.
Laustsen
L
L
Troelsen
J
J
D000626:Aminopeptidases / Q000235:genetics*; D000818:Animals; D018826:CD13 Antigens; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D051537:Hepatocyte Nuclear Factor 1; D051538:Hepatocyte Nuclear Factor 1-alpha; D051539:Hepatocyte Nuclear Factor 1-beta; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D013552:Swine; D014157:Transcription Factors / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C496487:HNF1A protein, human; C496495:HNF1B protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D009687:Nuclear Proteins; D014157:Transcription Factors; D051537:Hepatocyte Nuclear Factor 1; D051539:Hepatocyte Nuclear Factor 1-beta; D000626:Aminopeptidases; D018826:CD13 Antigens