Characterization of absorption and scattering properties for various yeast strains by time-resolved spectroscopy.
An understanding of the optical properties of biological media and cells is essential to the development of noninvasive optical studies of tissues. Unicellular organisms offer a unique opportunity to investigate the factors affecting light propagation, since they can be manipulated in ways impossible for more complex biological samples. In this study, we examined optical absorption and scattering properties of strongly multiple scattering yeast suspensions by means of near-infrared (NIR) time-resolved spectroscopy (TRS) and a sample substitution method. We determined the critical parameters for photon migration by varying the cell organelle content, the cell ploidy, the cell size, and the concentration of suspended cells. The results indicate that the photon absorption is insensitive to cell differentiation and that the cell volume is the primary factor determining light-scattering property.
Beauvoit
B
B
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 190104.
Liu
H
H
Kang
K
K
Kaplan
P D
PD
Miwa
M
M
Chance
B
B
D002452:Cell Count; D048429:Cell Size; D007424:Intracellular Fluid; D008027:Light; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D011003:Ploidies; D012542:Scattering, Radiation; D013055:Spectrophotometry, Infrared / Q000379:methods*; D015003:Yeasts / Q000737:chemistry / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*