title: Analysis of polymerase chain reaction product by capillary electrophoresis and its application to the detection of single base substitution in genes.
abstract: Capillary gel electrophoresis (CGE) was studied for the direct analysis of polymerase chain reaction (PCR) amplified samples. A low cross-linked polyacrylamide gel (3%T, 0.5%C) was used for CGE with treated and untreated silica capillaries. CGE showed high reproducibility and resolution in the separation of DNA fragments (ca. 100-1000 base pairs) produced by PCR. The CGE system was applied to the detection of an amplification refractory mutation system (ARMS) and PCR-restriction fragment length polymorphism (PCR-RFLP), which are detection methods of single base substitution in genes using PCR. With the CGE system, full automation of PCR product detection is feasible.
authors.affiliation: School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Arakawa
authors.forename: K
authors.initials: K
authors.lastname: Uetanaka
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: A
authors.initials: A
authors.lastname: Tsuji
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D004279:DNA, Viral / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D009154:Mutation; D010651:Phenylalanine Hydroxylase / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004279:DNA, Viral; D010651:Phenylalanine Hydroxylase; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases