A monoclonal antibody specific for a 40 kDa polypeptide recognizes pathogenic strains of Entamoeba histolytica.
In vitro interaction of Entamoeba histolytica trophozoites with collagen induces the intracellular formation and release of electron-dense granules (EDGs). We determined that four polypeptides in EDGs total antigen by SDS-PAGE were absent in trophozoite extracts. A monoclonal antibody raised against these EDGs recognized a polypeptide of 40 kDa specific for the pathogenic strains of E. histolytica. In addition, this mAb recognized a 96 kDa peptide masked by carbohydrates.
León
G
G
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN, Mexico City, Mexico.
Tovar
R
R
Muñoz
M L
ML
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000032:analysis; D003094:Collagen / Q000032:analysis / Q000378:metabolism; D004583:Electrons; D004748:Entamoeba histolytica / Q000276:immunology / Q000472:pathogenicity*; D015800:Protozoan Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D003094:Collagen