Identification and regional localization of a human IMP dehydrogenase-like locus (IMPDHL1) at 16p13.13.
Sequence-tagged sites (STSs) are versatile chromosomal markers for a variety of genome mapping efforts. In this report, we describe a randomly generated STS (323F4) from human chromosome 16 genomic DNA that has 90.0% sequence identity to the type I human inosine-5'-monophosphate dehydrogenase (IMPDH1) gene and 72% identity to the type II human inosine-5'-monophosphate dehydrogenase (IMPDH2) gene. Additional sequencing by primer walking has provided a total of 1380 bp of the human chromosome 16 sequence. The IMPDH-like sequence 323F4 was regionally localized by PCR analysis of a panel of somatic cell hybrids containing different portions of human chromosome 16 to 16p13.3-13.12, between the breakpoints found in hybrids CY196/CY197 and CY198. This regional mapping assignment was further refined to subband 16p13.13 by high-resolution fluorescence in situ hybridization using cosmid 323F4 as a probe. We conclude that a third, previously undescribed IMPDH locus, termed IMPDHL1, exists at human chromosome 16p13.13.
Doggett
N A
NA
Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
Callen
D F
DF
Chen
Z L
ZL
Moore
S
S
Tesmer
J G
JG
Duesing
L A
LA
Stallings
R L
RL
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D003360:Cosmids; D017931:DNA Primers; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D007168:IMP Dehydrogenase / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
D017931:DNA Primers; D007168:IMP Dehydrogenase