title: A novel AT-rich DNA binding protein that combines an HMG I-like DNA binding domain with a putative transcription domain.
abstract: There is growing evidence that AT-rich promoter elements play a role in transcription of plant genes. For the promoter of the nuclear gene for chloroplast glutamine synthetase from pea (GS2), the deletion of a 33-bp AT-rich sequence (box 1 native) from the 5' end of a GS2 promoter-beta-glucuronidase (GUS) fusion resulted in a 10-fold reduction in GUS activity. The box 1 native element was used in gel shift analysis and two distinct complexes were detected. One complex is related to the low-mobility complex reported previously for AT-rich elements from several other plant promoters. A multimer of the box 1 sequence was used to isolate a cDNA encoding an AT-rich DNA binding protein (ATBP-1). ATBP-1 is not a high-mobility group protein, but it is a novel protein that combines a high-mobility group I/Y-like DNA binding domain with a glutamine-rich putative transcriptional domain.
authors.affiliation: Department of Biology, New York University, New York 10003.
authors.forename: G
authors.initials: G
authors.lastname: Tjaden
authors.forename: G M
authors.initials: GM
authors.lastname: Coruzzi
mesh_terms: D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D007887:Fabaceae; D005966:Glucuronidase / Q000235:genetics; D005974:Glutamate-Ammonia Ligase / Q000235:genetics* / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D025741:HMGA1a Protein; D006609:High Mobility Group Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D014026:Nicotiana; D014158:Transcription, Genetic
chemical_list: C086324:ATBP-1 protein, tobacco; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D010940:Plant Proteins; D005973:Glutamine; D025741:HMGA1a Protein; D005966:Glucuronidase; D005974:Glutamate-Ammonia Ligase