title: Trans-splicing of a Meloidogyne incognita mRNA encoding a putative esophageal gland protein.
abstract: A monoclonal antibody, 7A9, specific for antigens in the subventral esophageal glands of adult female Meloidogyne incognita and for antigens in the longitudinal muscles of second-stage juveniles, was used to isolate a clone from a M. incognita cDNA expression library. The corresponding genomic DNA was isolated by hybridization and the gene designated sec-1. DNA sequence analysis of sec-1 revealed the presence of 9 introns having structural similarities to introns from the free-living nematode Caenorhabditis elegans. The sec-1 message was also trans-spliced and the leader sequence differed in one position from the sequence of the C. elegans trans-spliced leader SL1. Sequences analogous to sec-1 were specific to the genus Meloidogyne, and regulation of sec-1 expression did not involve differences in overall rates of transcript accumulation. The deduced amino acid sequence of the protein encoded by sec-1 had some similarities to the rod portions of several myosin heavy chains.
authors.affiliation: Department of Plant Pathology, University of Georgia, Athens 30602-7274.
authors.forename: C
authors.initials: C
authors.lastname: Ray
authors.forename: A G
authors.initials: AG
authors.lastname: Abbott
authors.forename: R S
authors.initials: RS
authors.lastname: Hussey
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000235:genetics; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D004947:Esophagus / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D010944:Plants / Q000469:parasitology; D012326:RNA Splicing; D018510:RNA, Helminth / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D014431:Tylenchoidea / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D018510:RNA, Helminth; D012333:RNA, Messenger