Molecular cloning and sequence analysis of the monkey and human tissue kallikrein genes.
Cynomolgus monkey renal kallikrein cDNA and genomic human tissue kallikrein gene were cloned. The monkey gene encodes a 257 amino acid (aa) preprokallikrein and exhibits 95% and 92% homology to the human at nucleotide (nt) and aa level, respectively. The monkey gene encodes a 233-aa mature kallikrein versus a 238-aa in human. The human kallikrein gene and urinary kallikrein both contain a Lys-162 instead of the reported Glu-162. Human, monkey and rat renal/pancreatic kallikrein genes evolve with a N-glycosylation containing domain (aa 81-87) which is absent in porcine and is non-glycosylable in mice. Only human kallikrein evolves with an additional Thr-108 and with a N-glycosylation site at aa-141.
Lin
F K
FK
Amgen Inc., Amgen Center, Thousand Oaks, CA 91320.
Lin
C H
CH
Chou
C C
CC
Chen
K
K
Lu
H S
HS
Bacheller
W
W
Herrera
C
C
Jones
T
T
Chao
J
J
Chao
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D006801:Humans; D007610:Kallikreins / Q000737:chemistry / Q000235:genetics*; D007668:Kidney / Q000378:metabolism*; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D007610:Kallikreins