Restriction fragment length polymorphism analysis of the chicken B-F and B-L genes and their association with serologically defined B haplotypes.
Seven serologically defined chicken haplotypes have been analysed by restriction fragment length polymorphism (RFLP) with chicken cDNA probes specific for MHC class I and II. The results demonstrate an excellent correlation between the observed RFLP banding patterns in the investigated haplotypes and the serological B-typing. In future, RFLP analysis in addition to serological B-typing may sharpen the tools in the search for recombinant chromosomes separating B-F and B-L.
Juul-Madsen
H R
HR
Royal Veterinary and Agricultural University, Frederiksberg C., Denmark.
Hedemand
J E
JE
Salomonsen
J
J
Simonsen
M
M
D000818:Animals; D015139:Blotting, Southern / Q000662:veterinary; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D006239:Haplotypes; D006650:Histocompatibility Testing / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length