Cloning, sequence and structural features of a lipase from the antarctic facultative psychrophile Psychrobacter immobilis B10.
A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35,288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold.
Arpigny
J L
JL
Laboratoire de Biochimie, Université de Liège-Sart Tilman, Belgium.
Feller
G
G
Gerday
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D005798:Genes, Bacterial; D008049:Lipase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data
D008049:Lipase