Evaluation of a 22 kDa Dirofilaria immitis antigen for the immunodiagnosis of human pulmonary dirofilariosis.
Human pulmonary dirofilariosis usually appears as a solitary pulmonary nodule. A 22 kDa antigen from Dirofilaria immitis adult worms specifically recognized by three sera from individuals diagnosed with pulmonary dirofilariosis was identified by enzyme-linked-immunoelectrotransfer blot. This antigen was purified by elution from acrylamide gels and evaluated in enzyme-linked immunoassay using sera from 92 patients with pulmonary dirofilariosis and other pulmonary and parasitic diseases. Its use in the diagnosis of human pulmonary dirofilariosis should be considered.
Perera
L
L
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Spain.
Muro
A
A
Cordero
M
M
Villar
E
E
Simón
F
F
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D004183:Dirofilaria immitis / Q000276:immunology*; D004184:Dirofilariasis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008174:Lung Diseases, Parasitic / Q000175:diagnosis*; D012698:Serologic Tests / Q000379:methods
D000947:Antigens, Helminth