Unchanged exocytotic release of glutamic acid in cortex and neostriatum of the rat during aging.
The Ca(2+)-dependent release of glutamate induced by 4-aminopyridine in synaptosomes prepared both from the cerebral cortex and basal ganglia was unchanged in aged rats (27-30 months) when compared to adults rats (3 months). Consistent with the absence of changes in glutamate exocytosis during aging, the rise in the cytosolic free Ca2+ concentration, [Ca2+]c, induced by depolarization in synaptosomes from aged rats was similar to that found in control adult rats. The results suggest that during aging the nerve terminals from the cerebral cortex and basal ganglia maintain an intact ability to release glutamate by exocytosis.
Sánchez-Prieto
J
J
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Herrero
I
I
Miras-Portugal
M T
MT
Mora
F
F
D015761:4-Aminopyridine / Q000494:pharmacology; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D005089:Exocytosis; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D008297:Male; D009411:Nerve Endings / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000378:metabolism
D005971:Glutamates; D018698:Glutamic Acid; D015761:4-Aminopyridine