A new high frequency polymorphism in the HER-2/neu oncogene in normal tissue and breast tumors.
The HER-2/neu (erbB-2) oncogene, if amplified and/or overexpressed in breast and ovarian cancers, is associated with a poor prognosis. Employing direct DNA sequencing, we have discovered and sequenced an 80 base pair intron from human placenta which contains an A to G polymorphism. This polymorphism lends itself to restriction fragment length polymorphism analysis of the PCR product spanning this intron. All three genotypes, homozygous A, heterozygous, and homozygous G appear in normal control populations and breast tumors. Also, no difference was seen between the polymorphic form found in five breast cancers and the corresponding normal tissue.
Briscoe
W T
WT
Cancer Treatment Research Foundation, Tulsa, OK 74137.
Ray
D B
DB
Airhart
J L
JL
Ratliff
A L
AL
Shockley
E A
EA
Whetsell
L
L
Schaefer
F V
FV
Williams
R M
RM
D001483:Base Sequence; D001940:Breast / Q000502:physiology*; D001943:Breast Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007438:Introns / Q000235:genetics; D008214:Lymphocytes / Q000737:chemistry; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases
D004273:DNA, Neoplasm; D015252:Deoxyribonucleases, Type II Site-Specific; D009856:Oncogene Proteins, Viral; D018719:Erb-b2 Receptor Tyrosine Kinases; C035801:CCSGG-specific type II deoxyribonucleases