Insulin-like growth factor I induced reduction in cysteine proteinase activity in freshly isolated proximal tubule cells of the rat.
The potential effects of insulin-like growth factor I (IGF-I) on lysosomal cysteine proteinases (cathepsin B, H and L+B activities) were investigated in the freshly isolated proximal tubule cells of rats. IGF-I significantly inhibited these enzyme activities after an incubation time of 80 min. This effect was associated with a dose-dependent increase in cellular protein content. The study suggests that, besides the established enhanced protein synthesis, IGF-I-induced cellular hypertrophy is mediated by a suppression of the proteolytic enzyme activity in proximal tubular cells.
Ling
H
H
Department of Nephrology, University of Würzburg, Germany.
Vamvakas
S
S
Schaefer
L
L
Schaefer
R M
RM
Teschner
M
M
Schramm
L
L
Heidland
A
A
D000818:Animals; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000187:drug effects* / Q000378:metabolism*; D006984:Hypertrophy / Q000201:enzymology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000201:enzymology* / Q000473:pathology; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D003546:Cysteine Endopeptidases