Isolation of a new H3.3 histone variant cDNA of P. lividus sea urchin: sequence and embryonic expression.
A cDNA encoding a new H3 histone variant has been isolated from a Paracentrotus lividus sea urchin embryo cDNA library. The encoded protein is identical to the H3.3 histone subtype identified in other species, with the difference that E replaces D at position 81. The clone corresponds to a transcript of about 1.6 kb, not dependent on DNA replication, present in the unfertilized egg and at all stages of embryonic development. The coding part of the cDNA cross-reacts also with a 0.5 kb H3 late histone mRNA.
Fucci
L
L
Department of Genetics, General and Molecular Biology, University of Naples Federico II, Italy.
Aniello
F
F
Branno
M
M
Biffali
E
E
Geraci
G
G
D000818:Animals; D001483:Base Sequence; D002970:Cleavage Stage, Ovum; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012617:Sea Urchins / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D006657:Histones; D012333:RNA, Messenger