Effects of halothane on the synthesis of neurotransmitter amino acids in mouse brain.
The effects of halothane on the synthesis of the three major neuroactive amino acids (gamma-aminobutyric acid, aspartate and glutamate) and glutamine, which is closely related metabolically, were investigated in mouse brain using a labelled precursor ([13C]glucose) and a gas chromatography-mass spectrometry system. The ratios of newly synthesized amino acids were increased relative to baseline values when animals were exposed to 1% halothane, and decreased when they were exposed to 2% halothane. These findings suggest that halothane affects the synthesis of neurotransmitter amino acids in a concentration-dependent manner, without discrimination between excitatory and inhibitory amino acids.
Watanabe
K
K
Department of Anesthesia, Kyoto University Hospital, Japan.
Arai
T
T
Aoki
M
M
Mori
H
H
Mori
K
K
D000818:Animals; D001224:Aspartic Acid / Q000096:biosynthesis*; D001923:Brain Chemistry / Q000187:drug effects*; D005971:Glutamates / Q000096:biosynthesis*; D018698:Glutamic Acid; D005973:Glutamine / Q000096:biosynthesis*; D006221:Halothane / Q000494:pharmacology*; D008297:Male; D051379:Mice; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
D005971:Glutamates; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D006221:Halothane