title: Micellar capillary electrophoresis separation and thermo-optical absorbance detection of products from manual peptide sequencing.
abstract: Micellar capillary electrophoresis is optimized for separation of phenylthiohydantoin (PTH) amino acids produced in manual Edman degradation reaction for protein sequencing. There are also two major side-products produced by the Edman degradation reaction: diphenylthiourea and dimethylphenylthiourea. We report the complete separation of 19 PTH amino acids plus the two major side-reaction products in 10 min. Capillary electrophoresis is used to identify the five residues generated by manual Edman degradation sequencing of a pentapeptide.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.forename: K C
authors.initials: KC
authors.lastname: Waldron
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: N J
authors.initials: NJ
authors.lastname: Dovichi
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification / Q000592:standards; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D010669:Phenylthiohydantoin / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000592:standards; D010670:Phenylthiourea / Q000031:analogs & derivatives / Q000302:isolation & purification; D012015:Reference Standards; D013890:Thiourea / Q000031:analogs & derivatives / Q000302:isolation & purification; D013946:Thymopoietins / Q000737:chemistry
chemical_list: D000596:Amino Acids; D008823:Micelles; D010446:Peptide Fragments; D010455:Peptides; D013946:Thymopoietins; D010669:Phenylthiohydantoin; D010670:Phenylthiourea; C005355:U 10157; C041898:splenopentin; C032788:diphenylthiourea; D013890:Thiourea