Depletion of secretory granules from the feline parotid gland: action of NANC transmitters per se.
A parotid acinar degranulation of approximately 60 and 40% was observed in cats under pentobarbitone anaesthesia after a 90-min period of continuous stimulation of the parasympathetic auriculo-temporal nerve at 10 Hz in the absence and presence of atropine, respectively. Atropine completely abolished the large fluid response of the gland to the nerve stimulation. In the non-atropinized cats, bethanechol, infused into the carotid artery at a dose rate evoking a salivary flow similar to that in response to parasympathetic nerve stimulation, caused an acinar degranulation of approximately 25% and acinar vacuolation. Vasoactive intestinal peptide (VIP; 0.5 microgram kg-1 min-1 also infused into the carotid artery for 90 min) caused an acinar degranulation of the same magnitude as the parasympathomimetic drug but the peptide did not give rise to any fluid secretion or vacuole formation. The experiments were performed in the presence of alpha- and beta-adrenoceptor blockers. Thus, in parotid glands of the cat, producing no overt secretion of fluid, non-adrenergic, non-cholinergic (NANC) mechanisms may be at work causing exocytosis of the acinar granules. These mechanisms are also likely to contribute to the secretion of granules in response to parasympathetic nerve activity in the absence of blockade of the classical autonomic receptors.
Ekström
J
J
Department of Pharmacology, University of Göteborg, Sweden.
Asztély
A
A
Helander
H F
HF
Tobin
G
G
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000008:administration & dosage / Q000494:pharmacology; D001341:Autonomic Nervous System / Q000502:physiology*; D001626:Bethanechol Compounds / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D002415:Cats; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000648:ultrastructure*; D004558:Electric Stimulation; D005260:Female; D006651:Histocytochemistry; D007261:Infusions, Intra-Arterial; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D010275:Parasympathetic Nervous System / Q000502:physiology; D010306:Parotid Gland / Q000187:drug effects / Q000294:innervation / Q000648:ultrastructure*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D001626:Bethanechol Compounds; D018377:Neurotransmitter Agents; D014660:Vasoactive Intestinal Peptide; D001285:Atropine