A rapid diagnostic assay for eastern equine encephalomyelitis viral RNA.
Eastern equine encephalomyelitis virus (EEEV) has been a low-frequency, but serious human and veterinary health problem. Increased frequency of this mosquito-borne virus is anticipated as wetlands are maintained and re-established. Control of EEEV has depended on mosquito abatement in response to increasing frequency of EEEV in the environment. A coupled reverse transcription/polymerase chain reaction assay was designed to rapidly, sensitively, and specifically detect EEEV RNA. The assay successfully detected the viral RNA in a single-blind study of a set of field samples composed of either pooled mosquitoes or bird tissue. These results suggest that it would be practical to use this assay for deciding when and where to implement mosquito abatement.
Vodkin
M H
MH
Department of Veterinary Pathobiology, Purdue University, West Lafayette, Indiana.
McLaughlin
G L
GL
Day
J F
JF
Shope
R E
RE
Novak
R J
RJ
D000818:Animals; D001483:Base Sequence; D001717:Birds; D009033:Culicidae; D017931:DNA Primers / Q000737:chemistry; D004663:Encephalitis Virus, Eastern Equine / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D015183:Restriction Mapping; D016037:Single-Blind Method; D013997:Time Factors
D017931:DNA Primers; D012367:RNA, Viral