title: Immunoelectron-microscopic quantitation of differential levels of chlamydial proteins in a cell culture model of persistent Chlamydia trachomatis infection.
abstract: Electron immunolabeling techniques were used for quantitative evaluation of alterations in the steady-state levels of chlamydial antigens in persistent Chlamydia trachomatis cultures. Gamma interferon-mediated persistent chlamydial development correlated with an increase in the levels of the chlamydial heat shock protein (hsp60) and with a significant reduction in the levels of the major outer membrane protein.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin, Madison 53706.
authors.forename: W L
authors.initials: WL
authors.lastname: Beatty
authors.forename: R P
authors.initials: RP
authors.lastname: Morrison
authors.forename: G I
authors.initials: GI
authors.lastname: Byrne
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D002478:Cells, Cultured; D018834:Chaperonin 60; D002692:Chlamydia trachomatis / Q000737:chemistry* / Q000254:growth & development; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D016253:Microscopy, Immunoelectron; D018272:Porins
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis; D007371:Interferon-gamma