Two Tcp-1-related but highly divergent gene families exist in oat encoding proteins of assumed chaperone function.
Tcp-1-related sequences have been isolated from a cDNA library of etiolated 6-day-old oat (Avena sativa) seedlings. This attempt was made to obtain cDNAs of a recently published 60 kDa plant chaperone that re-folds denatured phytochrome and which was biochemically characterised as a Tcp-1-related protein [(1993) Nature 363, 644-647]. The translation of the putative coding sequence from one full-length cDNA clone displays no specific homologies to amino acid sequences known from peptide sequencing of the oat 60 kDa chaperone. Antibodies raised against the 60 kDa chaperone and over-expressed protein from one full-length coding sequence for Tcp-1 from oat show no cross-reactivity, whereas a monoclonal antibody raised against mouse Tcp-1 protein recognizes both the 60 kDa protein purified from plant extracts and over-expressed protein from Tcp-1-related cDNA sequences.
Ehmann
B
B
Institut für Biologie II der Universität, Freiburg, Germany.
Krenz
M
M
Mummert
E
E
Schäfer
E
E
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins; D003429:Cross Reactions; D018076:DNA, Complementary; D002523:Edible Grain / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics* / Q000276:immunology
C085002:ASTCP-K19 protein, Avena sativa; D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D010940:Plant Proteins; D011506:Proteins; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins