Comparative analysis of the vagal stimulation of gastric acid secretion in rodent isolated stomach preparations.
1. Electrical field stimulation produced a tetrodotoxin-sensitive, frequency-dependent, release of acid from isolated, lumen-perfused, stomach preparations from mouse, immature rat and guinea-pig. 2. In the guinea-pig and mouse preparations, the frequency-dependent response was abolished by hexamethonium, acetylcholine (ACh) muscarinic (M) and histamine H2-receptor blockade, consistent with the hypothesis that the vagal ACh acts indirectly by stimulating the release of endogenous histamine. 3. In contrast, in the rat preparation the frequency-dependent response was partially refractory to all of these inhibitors. However, a combination of H2- and ACh M-receptor blockade did abolish the effect. 4. We conclude that vagal-stimulated acid secretion in the rat, unlike the other two species, behaves as though there is a direct innervation of the oxyntic cells by either cholinergic or noncholinergic neurones.
Welsh
N J
NJ
Department of Analytical Pharmacology, King's College School of Medicine & Dentistry, Rayne Institute, London.
Shankley
N P
NP
Black
J W
JW
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002766:Cholecystokinin / Q000037:antagonists & inhibitors; D018680:Cholinergic Antagonists; D004558:Electric Stimulation; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D006168:Guinea Pigs; D006584:Hexamethonium Compounds / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D008297:Male; D008536:Meglumine / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors; D013045:Species Specificity; D013779:Tetrodotoxin / Q000494:pharmacology; D014630:Vagus Nerve / Q000502:physiology*
D018680:Cholinergic Antagonists; D006584:Hexamethonium Compounds; D006635:Histamine H2 Antagonists; D007211:Indoles; D011949:Receptors, Cholecystokinin; C065599:PD 134308; D013779:Tetrodotoxin; D008536:Meglumine; D001285:Atropine; D002766:Cholecystokinin