Methionine enkephalin is hydrolyzed by aminopeptidase N on CD4+ and CD8+ spleen T cells.
Exogenous methionine enkephalin incubated with CD4+ or CD8+ T cells purified from murine spleen is metabolized primarily, if not exclusively, by aminopeptidase N (aminopeptidase M, EC 3.4.11.2), a membrane-anchored ectopeptidase. The enzyme activity is identified by its substrate specificity, sensitivity to inhibition by amastatin, and immunoreactivity with antibody to rat kidney aminopeptidase N. Activation of CD4+ T cells results in a small increase per cell in aminopeptidase N activity.
Miller
B C
BC
Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas 75235-9038.
Thiele
D L
DL
Hersh
L B
LB
Cottam
G L
GL
D000626:Aminopeptidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000906:Antibodies / Q000494:pharmacology; D018826:CD13 Antigens; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000201:enzymology; D016827:CD8 Antigens / Q000032:analysis; D002469:Cell Separation; D004744:Enkephalin, Methionine / Q000378:metabolism*; D006868:Hydrolysis; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009281:Naphthalenes / Q000378:metabolism; D010455:Peptides; D013154:Spleen / Q000166:cytology*; D013379:Substrate Specificity; D016176:T-Lymphocyte Subsets / Q000201:enzymology* / Q000276:immunology
D000900:Anti-Bacterial Agents; D000906:Antibodies; D017252:CD3 Complex; D016827:CD8 Antigens; D009281:Naphthalenes; D010455:Peptides; C072587:2-naphthylamide; D004744:Enkephalin, Methionine; C016977:amastatin; D000626:Aminopeptidases; D018826:CD13 Antigens