title: Carbon regulation of penicillin biosynthesis in Aspergillus nidulans: a minor effect of mutations in creB and creC.
abstract: Transcription of the Aspergillus nidulans ipnA gene is under carbon regulation. Loss-of-function mutations in creB or creC do not cause full derepression of ipnA transcript levels in sucrose-grown mycelia and do not elevate repressed penicillin levels, indicating that neither of these genes plays a major regulatory role in penicillin biosynthesis. However, these mutations reduce external pH acidification, accelerate sucrose degradation and result in extracellular accumulation of resulting D-glucose and D-fructose. These effects would explain the partial elevation of carbon-repressed ipnA transcript levels observed in strains carrying creB- or creC- mutations.
authors.affiliation: Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas del C.S.I.C., Madrid, Spain.
authors.forename: E A
authors.initials: EA
authors.lastname: Espeso
authors.forename: J M
authors.initials: JM
authors.lastname: Fernández-Cañón
authors.forename: M A
authors.initials: MA
authors.lastname: Peñalva
mesh_terms: D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004794:Enzyme Repression; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D010406:Penicillins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology
chemical_list: D010406:Penicillins; D012333:RNA, Messenger; D013395:Sucrose; D010088:Oxidoreductases; C038370:isopenicillin N synthetase