Effects of two neuronal antidiuretic molecules, neuroparsin and 5-hydroxytryptamine, on cytosolic free calcium monitored with indo-1 in epithelial and muscular cells of the African locust rectum.
Using the probe indo-1 in a microspectrofluorimetric study, it was demonstrated that the locust antidiuretic neurohormone, neuroparsin, enhanced cytosolic free Ca2+ concentrations, measured as percentages of the ratio F405/F480 (R), in epithelial cells of the African locust rectum. 5-hydroxytryptamine, whose antidiuretic effect was previously established, enhanced R in longitudinal muscular cells, and was able to increase R slightly in epithelial cells. The possibility of reciprocal Ca2+ movements between muscular and epithelial cells is discussed. Both of the neuronal molecules, which act via distinct transduction pathways (phosphoinositide turnover for neuroparsin, and Ca(2+)-dependent adenylate cyclase for 5-hydroxytryptamine), stimulated an increase in R by causing Ca2+ entry through dihydropyridine-sensitive Ca2+ channels. Cyclic nucleotides (cAMP and cGMP) lowered R in epithelial cells, the cGMP effect being interpreted as a feedback control on phosphoinositide turnover and resulting in the ability to re-establish cAMP production to levels incompatible with high PLC activity. In longitudinal muscular cells, the increase in R due to cAMP suggests the involvement of 5-hydroxytryptamine in stimulation of adenylate cyclase activity. Furthermore, results enabled the localization in epithelial cells of the transduction pathways mediating the actions of another antidiuretic factor extracted from the glandular lobes of the locust corpora cardiaca.
Fournier
B
B
Laboratoire de Neuroendocrinologie-U.R.A.C.N.R.S. 1138, Université de Bordeaux I, Talence, France.
Guérineau
N
N
Mollard
P
P
Girardie
J
J
D000818:Animals; D002118:Calcium / Q000032:analysis*; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D006110:Grasshoppers / Q000187:drug effects* / Q000648:ultrastructure; D007211:Indoles; D007301:Insect Hormones / Q000494:pharmacology*; D008297:Male; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology
D007211:Indoles; D007301:Insect Hormones; D018377:Neurotransmitter Agents; C058919:neuroparsin A; D012701:Serotonin; D000242:Cyclic AMP; D006152:Cyclic GMP; C048960:indo-1; D002118:Calcium