title: Cross-talk of the receptors for bradykinin, serotonin, and ATP shown by single cell Ca2+ responses indicating different modes of Ca2+ activation in a neuroblastoma x glioma hybrid cell line.
abstract: Modes of Ca2+ activation by bradykinin, serotonin, and ATP and the possible receptor cross-talk were investigated in mouse neuroblastoma x rat glioma hybrid cells (108CC15) by monitoring fura-2 fluorescence in single cells. A transient rise of cytosolic Ca2+ activity was induced by short pulses of the hormones. Brief exposure of cells to ionomycin, which depletes intracellular Ca2+ stores, reduced the size of subsequent responses to bradykinin or ATP, but not to serotonin. Superfusion of the cells with Ca(2+)-free medium abolished the Ca2+ response to serotonin, whereas the responses to bradykinin and to ATP were only slightly reduced. This indicates that ATP, like bradykinin, induces the release of Ca2+ from intracellular stores. Serotonin, in contrast, activates Ca2+ entry from the extracellular space. To investigate whether ATP releases Ca2+ from the same stores as bradykinin, we examined the interaction of the hormones by applying them consecutively. When ATP was applied after bradykinin, the nucleotide did not evoke any response, irrespective of the presence or absence of extracellular Ca2+. The application of ATP before that of bradykinin reduced the size of a following bradykinin-induced Ca2+ response in Ca(2+)-free medium, but not in Ca(2+)-containing medium. This suggests that bradykinin may interact with the ATP-activated mechanism by cross-desensitization. Possibly, bradykinin receptors are coupled to additional Ca2+ stores not accessible to ATP that are refilled by extracellular Ca2+. Cyclic AMP and cyclic GMP apparently do not affect the Ca2+ responses to bradykinin and serotonin, as shown by the lack of influence of preincubation of the cells with forskolin or sodium nitroprusside.
authors.affiliation: Physiologisch-chemisches Institut, Universität Tübingen, F.R.G.
authors.forename: G
authors.initials: G
authors.lastname: Reetz
authors.forename: G
authors.initials: G
authors.lastname: Reiser
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005910:Glioma; D006822:Hybrid Cells / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D015759:Ionomycin / Q000494:pharmacology; D051379:Mice; D009447:Neuroblastoma; D018377:Neurotransmitter Agents / Q000494:pharmacology; D009712:Nucleotides, Cyclic / Q000494:pharmacology; D051381:Rats; D018002:Receptors, Bradykinin / Q000378:metabolism*; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D018377:Neurotransmitter Agents; D009712:Nucleotides, Cyclic; D018002:Receptors, Bradykinin; D018048:Receptors, Purinergic P2; D011985:Receptors, Serotonin; D012701:Serotonin; D015759:Ionomycin; D001920:Bradykinin; D002118:Calcium