title: Induction of neutrophil respiratory burst by tumour necrosis factor-alpha; priming effect of solid-phase fibronectin and intervention of CD11b-CD18 integrins.
abstract: Human neutrophils, added to fibronectin (FN)-coated polystyrene wells and exposed to tumour necrosis factor-alpha (TNF-alpha), were found to exhibit a prolonged production of superoxide anion (O2-) after a lag period of approx 30 min. The O2- production, but not the cell adherence to FN, was completely inhibited by two MoAbs against CD18 and by a MoAb against CD11b, suggesting the involvement of CD11b-CD18 integrins in the neutrophil oxidative response. When neutrophils were induced to adhere to FN by incubation for 30 min on FN-coated surfaces and then washed to remove non-adherent cells, FN-anchored cells exhibited a rapid onset of O2- production in response to TNF-alpha. This suggests that FN primes neutrophils for the TNF-alpha-mediated respiratory burst. The O2- production by adherent neutrophils could be inhibited by anti-CD11b and anti-CD18 MoAbs only when the MoAbs were present both during the induction of adherence and during the subsequent exposure of FN-bound cells to TNF-alpha. The incapacity of MoAbs, added to neutrophils during the induction of adherence, to modify the characteristics of the subsequent neutrophil response to TNF-alpha suggests that the FN-mediated cell priming is independent of the interaction of CD11b-CD18 integrins with the FN substrate. The results are consistent with the intervention of three classes of cell receptors in the TNF-alpha-induced oxidative burst of neutrophils plated on FN: (i) neutrophil FN-binding sites, distinct from CD11b-CD18 and responsible for the cell priming; (ii) CD11b-CD18 integrins, absolutely required for permitting the cell triggering; and (iii) TNF-alpha receptors, responsible for switching on a rapid cell response in primed cells. The requirement of multiple classes of receptors for the full expression of the cell function can be envisaged as a natural precautionary measure to control the neutrophil responsiveness to TNF-alpha and, in turn, the TNF-alpha-dependent neutrophil-mediated oxidative injury at sites of inflammation.
authors.affiliation: Department of Internal Medicine, University of Genova Medical School, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Dapino
authors.forename: F
authors.initials: F
authors.lastname: Dallegri
authors.forename: L
authors.initials: L
authors.lastname: Ottonello
authors.forename: C
authors.initials: C
authors.lastname: Sacchetti
mesh_terms: D015703:Antigens, CD / Q000502:physiology*; D018821:CD18 Antigens; D002448:Cell Adhesion; D002478:Cells, Cultured; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D016023:Integrins / Q000502:physiology*; D016177:Macrophage-1 Antigen / Q000502:physiology; D008297:Male; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology; D016897:Respiratory Burst; D013481:Superoxides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D018821:CD18 Antigens; D005353:Fibronectins; D016023:Integrins; D016177:Macrophage-1 Antigen; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides