The human MCP-3 gene (SCYA7): cloning, sequence analysis, and assignment to the C-C chemokine gene cluster on chromosome 17q11.2-q12.
Monocyte chemotactic proteins (MCPs) are chemokines involved in macrophage recruitment during inflammation and cancer. A full-size MCP-3 cDNA was used to isolate the functional human MCP-3 gene. Based on restriction analysis, subclones were selected and the MCP-3 gene sequence was completed. In addition to a dense region with direct and inverted repeats and palindromic sequences, a double microsatellite (CA)n-(GA)n' was found at the 5'-end of the MCP-3 gene, and an RFLP was detected. The gene was regionally mapped by fluorescence in situ hybridization to human chromosome 17, subbands q11.2-q12. This site contains the MCP-subset of C-C chemokines and can be distinguished from the syntenic MIP-1 alpha locus. SCYA7 was assigned as the locus symbol of the MCP-3 gene. Double-labeling experiments confirmed the regional assignment of the MCP-3 gene close to the ERBB2 locus on human chromosome 17.
Opdenakker
G
G
Rega Institute, University of Leuven, Belgium.
Fiten
P
P
Nys
G
G
Froyen
G
G
Van Roy
N
N
Speleman
F
F
Laureys
G
G
Van Damme
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D054410:Chemokine CCL7; D002630:Chemotactic Factors / Q000235:genetics*; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D016384:Consensus Sequence; D016207:Cytokines / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D018945:Monocyte Chemoattractant Proteins; D005810:Multigene Family; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D011518:Proto-Oncogene Proteins / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D054410:Chemokine CCL7; D002630:Chemotactic Factors; D016207:Cytokines; D004276:DNA, Satellite; D015245:Deoxyribonuclease BamHI; D066246:ErbB Receptors; D018945:Monocyte Chemoattractant Proteins; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; C515450:CCL7 protein, human