Heat shock response in Actinobacillus actinomycetemcomitans.
The heat shock response in Actinobacillus actinomycetemcomitans, a capnophilic Gram-negative bacterial species that is implicated in the development of certain forms of periodontitis, was characterized. Different strains of A. actinomycetemcomitans were grown at 37, 42 and 48 degrees C in the presence of 35S-methionine. The bacterial cells were lysed, run on SDS-PAGE and subsequently blotted on nitrocellulose paper. After autoradiography of the blots, several protein bands from the cultures at 42 degrees C showed an increased intensity; major bands were observed at 90, 70, and 60 kDa, but increased protein synthesis was also detected at 54, 28 and 17 kDa. Nitrocellulose blots were also incubated with a panel of monoclonal and polyclonal antibodies directed to epitopes on different heat shock proteins. Strong reactivity was found with several antibodies at the position corresponding to a molecular mass of 60 kDa. The protein is probably the GroEL homologue in A. actinomycetemcomitans, a member of the 'common bacterial antigen' family.
Løkensgard
I
I
Department of Oral Biology, Dental Faculty, University of Oslo, Norway.
Bakken
V
V
Schenck
K
K
D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology / Q000378:metabolism*; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000276:immunology; D018834:Chaperonin 60; D004591:Electrophoresis, Polyacrylamide Gel; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000502:physiology; D015151:Immunoblotting
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins