OR-1, a member of the nuclear receptor superfamily that interacts with the 9-cis-retinoic acid receptor.
We have cloned a member of the nuclear receptor superfamily. The cDNA was isolated from a rat liver library and encodes a protein of 446 aa with a predicted mass of 50 kDa. This clone (OR-1) shows no striking homology to any known member of the steroid/thyroid hormone receptor superfamily. The most related receptor is the ecdysone receptor and the highest homologies represent < 10% in the amino-terminal domain, between 15-37% in the carboxyl-terminal domain and 50-62% in the DNA binding domain. The expression of OR-1 appears to be widespread in both fetal and adult rat tissues. Potential DNA response elements composed of a direct repeat of the hexameric motif AGGTCA spaced by 0-6 nt were tested in gel shift experiments. OR-1 was shown to interact with the 9-cis-retinoic acid receptor (retinoid X receptor, RXR) and the OR-1/RXR complex to bind to a direct repeat spaced by 4 nt (DR4). In transfection experiments, OR-1 appears to activate RXR-mediated function through the DR4. Therefore OR-1 might modulate 9-cis-retinoic acid signaling by interacting with RXR.
Teboul
M
M
Center for Biotechnology, Karolinska Institute, NOVUM, Huddinge, Sweden.
Enmark
E
E
Li
Q
Q
Wikström
A C
AC
Pelto-Huikko
M
M
Gustafsson
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000378:metabolism; D005333:Fetus; D015723:Gene Library; D017403:In Situ Hybridization; D007536:Isomerism; D008099:Liver / Q000378:metabolism*; D000071518:Liver X Receptors; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000096:biosynthesis* / Q000378:metabolism*; D014162:Transfection; D014212:Tretinoin / Q000378:metabolism
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D000071518:Liver X Receptors; C000605212:Nr1h2 protein, rat; D015345:Oligonucleotide Probes; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D018168:Receptors, Retinoic Acid; D014212:Tretinoin