Effect of putrescine on tissue transglutaminase activity in wounds: decreased breaking strength and increased matrix fucoprotein solubility.
Topical application of putrescine, a transglutaminase inhibitor, for 3 days directly to rat skin wounds produced a significant average decrease of 48 percent in wound breaking strength in test animals from 8 pairs studied between day 5 and day 10 after wounding. No external or systemic toxic effects of putrescine were seen with localized topical application of 50 mM putrescine for 3 days in any of the test rats (n = 12), and no systemic toxicity was seen in rabbits (n = 4) after topical exposure to 50 mM putrescine for 3 weeks. Quantitation of tritiated fucose incorporation in rat wound explants from 10 pairs of rats revealed that a significant overall decrease in radiolabeled glycoprotein production of 23 percent occurred when putrescine was present; in addition, the fraction of tritiated glycoprotein which was soluble in buffer was significantly increased, while that in the buffer-insoluble fraction decreased. This study suggests that putrescine inhibits tissue transglutaminase-mediated cross-linking of fucoprotein in the extracellular wound matrix and supports a role for this process in the generation of incisional wound strength.
Dolynchuk
K N
KN
Department of Surgery, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Bendor-Samuel
R
R
Bowness
J M
JM
D000818:Animals; D003094:Collagen; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis / Q000737:chemistry* / Q000187:drug effects; D011700:Putrescine / Q000494:pharmacology* / Q000633:toxicity; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects / Q000201:enzymology* / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D012995:Solubility; D013314:Stress, Mechanical; D013718:Tensile Strength; D013997:Time Factors; D011503:Transglutaminases / Q000037:antagonists & inhibitors*; D014316:Tritium; D014945:Wound Healing
D003432:Cross-Linking Reagents; D006023:Glycoproteins; C016465:fucoproteins; D014316:Tritium; D003094:Collagen; D011503:Transglutaminases; D011700:Putrescine