Expression of glnA in the cyanobacterium Synechococcus sp. strain PCC 7942 is initiated from a single nif-like promoter under various nitrogen conditions.
The glnA mRNA, encoding glutamine synthetase, is differentially accumulated in the cyanobacterium Synechococcus sp. strain PCC 7942 in media containing different nitrogen sources. With the different nitrogen compounds, transcription of glnA initiated at a single site located -146 nucleotides upstream of the translation start site of the gene. A similarity of the nif-like promoter of the glnA gene of Anabaena sp. strain PCC 7120 and a binding-site sequence for the Synechococcus sp. strain PCC 7942 transcription regulator, NtcA, were found upstream of the transcription initiation site.
Cohen-Kupiec
R
R
Department of Botany, Tel Aviv University, Ramat-Aviv, Israel.
Gurevitz
M
M
Zilberstein
A
A
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002455:Cell Division; D000458:Cyanobacteria / Q000235:genetics* / Q000254:growth & development; D004268:DNA-Binding Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial; D005974:Glutamate-Ammonia Ligase / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D009566:Nitrates / Q000494:pharmacology; D017672:Nitrogen Compounds / Q000494:pharmacology*; D009586:Nitrogen Fixation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D009566:Nitrates; D017672:Nitrogen Compounds; D000644:Quaternary Ammonium Compounds; D012333:RNA, Messenger; D014157:Transcription Factors; C075617:ntcA protein, Synechococcus; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase