Secretion of alpha 2-plasmin inhibitor is impaired by amino acid deletion in a small region of the molecule.
Alpha 2-plasmin inhibitor (alpha 2PI) deficiency Okinawa results from defective secretion of the inhibitor from the liver and appears to be a direct consequence of the deletion of Glu137 in the amino acid sequence of alpha 2PI. To examine the effects of replacing the amino acid occupying position 137 or deleting its neighboring amino acid on alpha 2PI secretion, we used oligonucleotide-directed mutagenesis of alpha 2PI cDNA to change the codon specifying Glu137 or delete a codon specifying its neighboring amino acid. The effects were determined by pulse-chase experiments and by enzyme-linked immunosorbent assay of media from transiently transfected COS-7 cells. Replacement of Glu137 with an amino acid other than Cys had little effect on alpha 2PI secretion. In contrast, deletion of an amino acid in a region spanning a sequence of less than 30 amino acids including positions 127 and 137 severely impaired the secretion. The results suggest that structural integrity of the region, rather than its component amino acids, is important for the intracellular transport and secretion of alpha 2PI.
Toyota
S
S
First Department of Internal Medicine, Tokyo Medical and Dental University.
Hirosawa
S
S
Aoki
N
N
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003545:Cysteine / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005971:Glutamates / Q000737:chemistry*; D018698:Glutamic Acid; D000882:Haplorhini; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D014162:Transfection; D000979:alpha-2-Antiplasmin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D018076:DNA, Complementary; D005971:Glutamates; D000979:alpha-2-Antiplasmin; D018698:Glutamic Acid; D003545:Cysteine