title: Solid-phase extraction and reversed-phase high-performance liquid chromatographic determination of sulphur mustard in blood.
abstract: A reversed-phase high-performance liquid chromatographic method is reported for the analysis of sulphur mustard in blood with the aid of solid-phase extraction sample preparation. Sulphur mustard is extracted from blood samples (both in vitro and in vivo) of rats with a solution of 0.05 M sodium dodecyl sulphate and pre-concentrated over Sep-Pak C18 cartridges pre-coated with Tween-20. A Polygosil C18 column is used with acetonitrile-water (52:48, v/v) as mobile phase for separation and sulphur mustard was detected at 200 nm.
authors.affiliation: Defence Research and Development Establishment, Gwalior, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Dangi
authors.forename: K
authors.initials: K
authors.lastname: Jeevaratnam
authors.forename: K
authors.initials: K
authors.lastname: Sugendran
authors.forename: R C
authors.initials: RC
authors.lastname: Malhotra
authors.forename: C D
authors.initials: CD
authors.lastname: Raghuveeran
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009151:Mustard Gas / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009151:Mustard Gas