title: Crystallization and preliminary X-ray analysis of gamma-glutamyltranspeptidase from Escherichia coli K-12.
abstract: gamma-Glutamyltranspeptidase (EC 2.3.2.2) from Escherichia coli K-12 has been purified and crystallized by means of vapor diffusion in hanging drops. Two kinds of crystals on cell dimensions were found for X-ray diffraction analysis, one from ammonium sulfate and the other from polyethylene glycol 6000 as precipitants. The crystals of the orthorhombic form grown in the presence of 15% polyethylene glycol and 20 mM sodium acetate buffer were chosen for further analysis. The crystals belonged to space group P2(1)2(1)2(1), with cell dimensions of a = 128.1, b = 129.9 and c = 79.2 A, and two molecules constitute an asymmetric unit. These crystals diffracted to 2.0 A resolution and were suitable for X-ray crystallographic studies.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kumagai
authors.forename: S
authors.initials: S
authors.lastname: Nohara
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: W
authors.initials: W
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: K
authors.initials: K
authors.lastname: Sakabe
authors.forename: K
authors.initials: K
authors.lastname: Fukuyama
authors.forename: N
authors.initials: N
authors.lastname: Sakabe
mesh_terms: D001426:Bacterial Proteins; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology; D011994:Recombinant Proteins; D005723:gamma-Glutamyltransferase / Q000648:ultrastructure*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D005723:gamma-Glutamyltransferase