Effect of infection by lactic dehydrogenase virus on expression of intercellular adhesion molecule-1 on vascular endothelial cells of pancreatic islets in streptozotocin-induced insulitis of CD-1 mice.
Streptozotocin (SZ)-induced insulitis, which is an animal model for insulin-dependent diabetes mellitus, was suppressed by lactic dehydrogenase virus (LDV) infection. There were no differences in degenerative and necrotic changes of islet cells between SZ-treated mice and SZ-treated mice with LDV infection during the pre-insulitis phase. The degree of insulitis was more severe and the plasma glucose levels were higher in SZ-treated mice than in SZ-treated mice with LDV infection. Severe degenerative and necrotic changes of cells with mononuclear cell infiltration into the islets were seen in the SZ-treated mice. Infiltration of these cells into islets was less in SZ-treated mice with LDV infection. During the pre-insulitis phase, there was slight expression of intercellular adhesion molecule-1 (ICAM-1) on the surface of vascular endothelial cells in and/or around islets in both groups. ICAM-1 expression on vascular endothelial cells increased in parallel to the degree of insulitis. The degree of this expression in SZ-treated mice was higher than in SZ-treated mice with LDV infection. These results suggest that expression of ICAM-1 on vascular endothelial cells in SZ-treated mice may be important for the development of insulitis. Also, decreased expression of ICAM-1 in the islets may be responsible for the inhibition of the development of insulitis seen in SZ-treated mice with LDV infection.
Hayashi
T
T
Laboratory of Veterinary Pathology, Yamaguchi University, Japan.
Hashimoto
S
S
Kawashima
A
A
D000818:Animals; D018174:Arterivirus Infections / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D001786:Blood Glucose / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1; D007515:Islets of Langerhans / Q000276:immunology / Q000473:pathology; D007772:Lactate dehydrogenase-elevating virus; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
D001786:Blood Glucose; D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1