title: Differential expression of glycine receptor subunits in the retina of the rat: a study using immunohistochemistry and in situ hybridization.
abstract: Immunohistochemistry and in situ hybridization were used to study the distribution of glycine receptor (GlyR) subunits and the GlyR-associated protein gephyrin in the rat retina. Monoclonal antibodies against the alpha and beta subunits of the GlyR and gephyrin showed a strong punctate labeling pattern in the inner plexiform layer. Glycine receptor mRNAs were found in the inner nuclear layer and the ganglion cell layer. The alpha 1 subunit mRNA is predominantly present in the outer half of the INL and on some but not all ganglion cells. GlyR alpha 2 subunit mRNA is predominantly present in the inner half of the INL and on nearly all cells in the ganglion cell layer. GlyR alpha 3-, GlyR beta-, and gephyrin-mRNAs are present in the entire INL and in cells in the ganglion cell layer. The differential expression of glycine receptor subunits indicates a functional diversity of glycine receptors in the retina.
authors.affiliation: Neuroanatomische Abteilung, Max-Planck-Institut für Hirnforschung, Frankfurt, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Greferath
authors.forename: J H
authors.initials: JH
authors.lastname: Brandstätter
authors.forename: H
authors.initials: H
authors.lastname: Wässle
authors.forename: J
authors.initials: J
authors.lastname: Kirsch
authors.forename: J
authors.initials: J
authors.lastname: Kuhse
authors.forename: U
authors.initials: U
authors.lastname: Grünert
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D017403:In Situ Hybridization; D007395:Interneurons / Q000737:chemistry; D008565:Membrane Proteins / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D018009:Receptors, Glycine / Q000032:analysis*; D012160:Retina / Q000737:chemistry*; D012165:Retinal Ganglion Cells / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D018009:Receptors, Glycine; C073441:gephyrin