Significance of VLA-4 and LFA-1 expressions in neoplastic follicle formation and its deterioration in B-cell non-Hodgkin's lymphomas.
In an attempt to determine the roles of adhesion molecules in the formation and deterioration of neoplastic follicles, we used flow cytometry to investigate how strongly neoplastic B-cells express VLA-4 alpha and LFA-1 alpha on their surfaces. Neoplastic and normal B-cells were taken from 24 patients with B-cell non-Hodgkin's lymphomas (B-NHL) and 6 with B-cell chronic lymphocytic leukemia (B-CLL). The expression intensities of the adhesion molecules were graded as follows: (-), (+), (+2) and (+3). Normal B-cells expressed those molecules with an intensity of (+2). The data for VLA-4 alpha expression were as follows: follicular B-NHL [10/11; (+2) and 1/11; (+)], partially follicular [5/5; (+)], diffuse [8/8; (+)] and B-CLL [6/6; (-)]. Those for LFA-1 alpha were as follows: follicular B-NHL [7/11; (+2), 4/11; (+)], partially follicular [3/5; (+2), 2/5; (+)], diffuse [3/8; (+2), 5/8; (+)] and B-CLL [3/6; (+), 3/6; (-)]. These results suggest that VLA-4 molecules expressed on neoplastic B-cells may be involved closely in the formation and deterioration of neoplastic follicles, although the expression of LFA-1 molecules seems to play only a minor part in such events.
Kuriyama
Y
Y
Department of Internal Medicine, Tokyo Medical College, Japan.
Nakano
M
M
Kawanishi
Y
Y
Iwase
O
O
Kuge
S
S
Toyama
K
K
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping; D018799:Intercellular Adhesion Molecule-1; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology* / Q000473:pathology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis / Q000096:biosynthesis*; D016393:Lymphoma, B-Cell / Q000145:classification / Q000276:immunology* / Q000473:pathology; D008224:Lymphoma, Follicular / Q000276:immunology / Q000473:pathology; D016029:Receptors, Very Late Antigen / Q000032:analysis / Q000096:biosynthesis*; D012016:Reference Values
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D016029:Receptors, Very Late Antigen; D018799:Intercellular Adhesion Molecule-1