[Thyrotropin receptor peptide antibody with thyroid stimulating activity induces an experimental hyperthyroidism in rabbits].
It has been presumed that N-terminal region of the TSH-R is important for TSH or TSH-R autoantibody binding to the receptor. We have synthesized human TSH-R peptide corresponding to the N-terminus of the extracellular domain, and immunized it into 8 rabbits. All these rabbits produced antibody against the peptide. Seven out of 8 antibodies showed thyroid stimulating antibody (TSAb) activity. We found that T3 and T4 concentrations in the sera of the immunized rabbits were significantly higher than those of the pre-immune sera, and increased time-dependently. These results suggest that anti-TSH-R peptide antibody with TSAb activity induced an increase in thyroid hormones in rabbits.
Ohmori
M
M
Third Department of Internal Medicine, University of Yamanashi Medical School.
Endo
T
T
Onaya
T
T
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D006801:Humans; D006980:Hyperthyroidism / Q000209:etiology* / Q000276:immunology; D018828:Immunoglobulins, Thyroid-Stimulating; D010455:Peptides / Q000276:immunology; D011817:Rabbits; D011989:Receptors, Thyrotropin / Q000276:immunology*; D013972:Thyrotropin / Q000276:immunology*
D001323:Autoantibodies; D018828:Immunoglobulins, Thyroid-Stimulating; D010455:Peptides; D011989:Receptors, Thyrotropin; D013972:Thyrotropin