Ammonium assimilation in Nocardia asteroides.
Specific enzymes of ammonium assimilation were measured in cell-free extracts of Nocardia asteroides grown in a synthetic medium with glutamate as the nitrogen source. Cell-free extracts had active glutamine synthetase (GS) and glutamate synthase (GOGAT) and alanine dehydrogenase (ADH) but glutamate dehydrogenase (GDH) could not be detected in the enzyme preparation. This shows that GS/GOGAT is the major pathway of ammonium assimilation in N. asteroides.
Palaniappan
C
C
Department of Biology, Fisk University, Nashville, Tennessee.
Gunasekaran
M
M
D050788:Alanine Dehydrogenase; D000594:Amino Acid Oxidoreductases / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000032:analysis; D005970:Glutamate Synthase / Q000032:analysis; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D009616:Nocardia asteroides / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*
D005971:Glutamates; D000644:Quaternary Ammonium Compounds; D018698:Glutamic Acid; D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; D005947:Glucose