Hydrophilization of porous polystyrene-based continuous rod column.
Reactive porous rods of poly[(chloromethyl)styrene-co-divinylbenzene] have been prepared for the first time by copolymerization of the monomers in the presence of a porogenic diluent. A two-step modification process involving reaction with ethylenediamine followed by reaction with gamma-gluconolactone leads to a porous medium that has highly hydrophilic surface functionalities. Preliminary chromatographic evaluation with both small molecules such as alkylbenzenes in reversed-phase mode and large proteins in hydrophobic adsorption and ion-exchange modes demonstrates that the surface of the modified rods possesses a hydrophilicity that is comparable to that of the best hydrophilic HPLC packings.
Wang
Q C
QC
Department of Chemistry, Baker Laboratory, Cornell University, Ithaca, New York 14853-1301.
Svec
F
F
Fréchet
J M
JM
D002851:Chromatography, High Pressure Liquid; D011108:Polymers; D020058:Styrene; D013343:Styrenes / Q000737:chemistry*
D011108:Polymers; D013343:Styrenes; D020058:Styrene