title: Localization of PDGF A and PDGFR alpha mRNA in Xenopus embryos suggests signalling from neural ectoderm and pharyngeal endoderm to neural crest cells.
abstract: In situ hybridization analysis of Xenopus laevis embryos reveals that mRNA encoding the platelet-derived growth factor alpha receptor (PDGFR alpha) is expressed in cephalic neural crest masses prior to migration from the future neural tube and during their migration into the visceral arches. The analysis of fluorescently labeled neural crest tissue transplanted to unlabeled host embryos demonstrates that neural crest cells are the only detectable source of PDGFR alpha mRNA within visceral arches. Transcripts encoding PDGF A are present in neural ectoderm, otic vesicle and pharyngeal endoderm. Their location suggests that PDGF A provides a signal, first from the neural epithelium and later from the otic vesicle and pharyngeal endoderm, to cephalic neural crest cells during their migration in the arch region.
authors.affiliation: Department of Pharmacology, Cornell University Medical College, New York, NY 10021.
authors.forename: L
authors.initials: L
authors.lastname: Ho
authors.forename: K
authors.initials: K
authors.lastname: Symes
authors.forename: C
authors.initials: C
authors.lastname: Yordán
authors.forename: L J
authors.initials: LJ
authors.lastname: Gudas
authors.forename: M
authors.initials: M
authors.lastname: Mercola
mesh_terms: D000818:Animals; D004475:Ectoderm / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D004707:Endoderm / Q000378:metabolism; D017403:In Situ Hybridization; D009432:Neural Crest / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D010614:Pharynx / Q000196:embryology / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D014982:Xenopus laevis
chemical_list: D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D017479:Receptors, Platelet-Derived Growth Factor