NF-kappa B-dependent and -independent pathways of HIV activation in a chronically infected T cell line.
J delta K cells were isolated as a chronically infected survivor cell line, following infection of Jurkat CD4+ T cells with dl-NF, a mutated strain of human immunodeficiency virus type 1 (HIV-1) containing a deletion of the long terminal repeat (LTR) NF-kappa B sites. J delta K cells exhibited very low levels of constitutive HIV production. HIV-1 expression was activated from J delta K cells by treatment with phorbol myristate acetate (PMA), sodium butyrate (NaB), or hexamethylene bisacetamide (HMBA), but not tumor necrosis factor alpha (TNF-alpha), confirming the role of NF-kappa B in mediating TNF-alpha induction of HIV transcription. The strong induction of HIV expression by NaB or HMBA in J delta K cells clearly demonstrates the existence of NF-kappa B-independent mechanisms of HIV activation in chronically infected cells. J delta K cells may provide a useful model for characterizing NF-kappa B-independent transcriptional activation of the HIV LTR.
Antoni
B A
BA
New Jersey Center for Advanced Biotechnology and Medicine (CABM), Department of Viral Pathogenesis, Piscataway.
Rabson
A B
AB
Kinter
A
A
Bodkin
M
M
Poli
G
G
D000081:Acetamides / Q000494:pharmacology; D001483:Base Sequence; D001665:Binding Sites; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D016325:HIV Long Terminal Repeat / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D000081:Acetamides; D002087:Butyrates; D016328:NF-kappa B; D012333:RNA, Messenger; D020148:Butyric Acid; C014026:hexamethylene bisacetamide