title: A fourth secreted aspartyl proteinase gene (SAP4) and a CARE2 repetitive element are located upstream of the SAP1 gene in Candida albicans.
abstract: Candida albicans secreted aspartyl proteinases (Sap), products of the SAP genes, which are presumed to act as virulence factors. In the C. albicans strain WO-1, the ability to secrete Sap1 is regulated with switch phenotype, another putative virulence factor. KpnI restriction fragment length polymorphisms differentiate between several distinct SAP1 alleles in laboratory and clinical strains. Both SAP1 alleles from strain WO-1 along with their 5'- and 3'-flanking regions were cloned and sequenced, as were both alleles from another strain, SS. The 5'-flanking regions were remarkably similar in all four of the sequenced alleles over approximately 1,500 nucleotides. S1 analysis revealed that both alleles of WO-1 are transcribed. Characterization of the one allele from strain WO-1 identified a 284-nucleotide insertion flanked by 8-bp direct repeats that shows homology to the CARE2 repetitive element and that is not present in the other alleles. Characterization of the SAP1 alleles also identified a fourth SAP gene (SAP4) that includes an extended leader sequence. SAP4 is positioned upstream, in tandem to SAP1, in all strains tested and may encode another closely related secreted aspartyl proteinase.
authors.affiliation: Department of Pharmaceutical Chemistry, University of California at San Francisco 94118.
authors.forename: S H
authors.initials: SH
authors.lastname: Miyasaki
authors.forename: T C
authors.initials: TC
authors.lastname: White
authors.forename: N
authors.initials: N
authors.lastname: Agabian
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics* / Q000472:pathogenicity; D004251:DNA Transposable Elements / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D004271:DNA, Fungal; D005656:Fungal Proteins; D016282:Aspartic Acid Endopeptidases; C086390:SAP1 protein, Candida albicans; C086392:SAP4 protein, Candida albicans