[Establishment of monoclonal antibody mNI-11 which induces homotypic cell aggregation of LPS-treated U937 cells].
A mouse monoclonal antibody (mAb), designated as mNI-11, was produced. The reactivity of mNI-11 was assessed by immunofluorescence assay with various cells. Myelomonocytic cell line, U937, and lipopolysaccharide (LPS)-treated U937 (LPS-U937 cells) were found to bind strongly to the mAb. EBV-B cell line and peripheral blood mononuclear cells (PBMCs) were found to bind moderately to it. This mAb strongly induced homotypic cell aggregation of LPS-U937 cells. The mAbs to CD18 and CD54 completely inhibited the LPS-U937 cell aggregation induced by mNI-11. The surface antigens of the U937 and LPS-U937 cells recognized by mNI-11 had a molecular size of 95-97 kDa as determined by immunoblotting analysis.
Ikewaki
N
N
Department of Infections, School of Nursing, Kitasato University, Kanagawa, Japan.
Sasahara
T
T
Tamauchi
H
H
Inoko
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD; D000954:Antigens, Surface / Q000276:immunology; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D002449:Cell Aggregation; D002478:Cells, Cultured; D018799:Intercellular Adhesion Molecule-1; D008070:Lipopolysaccharides; D051379:Mice; D008970:Molecular Weight; D009000:Monocytes / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D008070:Lipopolysaccharides; D018799:Intercellular Adhesion Molecule-1