Uptake and transport of copolymer biodegradable microspheres by rabbit Peyer's patch M cells.
In this study, we demonstrate the role of M cells in uptake of poly(D-L-lactic-co-glycolic acid) (PLGA) microspheres and transport into rabbit Peyer's patches. Microspheres 1 to 10 microns in diameter composed of 50:50 lactic acid:glycolic acid were instilled into intestinal segments containing jejunal or ileal Peyer's patches, and uptake by M cells was examined by electron microscopy. PLGA microspheres visualized as electron-lucent, spherical particles were taken up by M cells by pseudopod-like extensions of the M cell apical membrane and translocated to the pocket region containing mononuclear leukocytes within 60 min. These results indicate that PLGA microspheres can be directed to M cell apical surfaces for delivery to immunocompetent cells in gut-associated lymphoid tissues.
Ermak
T H
TH
Vaccine Delivery Research Section, OraVax Inc., Cambridge, MA 02139.
Dougherty
E P
EP
Bhagat
H R
HR
Kabok
Z
Z
Pappo
J
J
D000818:Animals; D001673:Biodegradation, Environmental; D001692:Biological Transport; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005260:Female; D019344:Lactic Acid; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008863:Microspheres; D010581:Peyer's Patches / Q000378:metabolism*; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000493:pharmacokinetics*; D011817:Rabbits
D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid