Generation and molecular analyses of two rheumatoid synovial fluid-derived IgG rheumatoid factors.
OBJECTIVE
To study the Ig genes that encode IgG rheumatoid factor (IgG-RF) from rheumatoid synovial fluid.


METHODS
We used rheumatoid synovial fluid B cells to generate IgG-RF-secreting hybridomas. We then characterized their binding properties and determined their nucleotide sequences.


RESULTS
Two monospecific IgG-RFs were obtained. Sequence analysis of the RFs revealed a new V lambda gene family (designated V lambda 9) and extensive somatic diversification, including a duplication-insertion of 18 nucleotides (6 amino acid residues) into a hypervariable region.


CONCLUSIONS
The data provide further support for an antigen-driven response in the sustained production of potentially pathogenic IgG-RFs in rheumatoid synovium.
Lu
E W
EW
Department of Medicine, University of California, San Diego.
Deftos
M
M
Olee
T
T
Huang
D F
DF
Soto-Gil
R W
RW
Carson
D A
DA
Chen
P P
PP
D000595:Amino Acid Sequence; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001402:B-Lymphocytes / Q000378:metabolism; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012217:Rheumatoid Factor / Q000032:analysis* / Q000378:metabolism; D013582:Synovial Fluid / Q000276:immunology*
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D004247:DNA; D012217:Rheumatoid Factor