title: ICAM-1 expression on human brain microvascular endothelial cells.
abstract: There is evidence that leukocytes play an important role in mediating tissue injury during acute ischemic stroke. Endothelial cell adhesive molecules such as ICAM-1 are required for the migration of leukocytes into the brain. Using an Elisa, we compared the expression of ICAM-1 by human brain microvascular endothelial cells with human umbilical vein endothelial cells. There was constitutive surface expression of ICAM-1 on both brain and umbilical vein endothelial cells. With cytokine (IL-1 beta or TNF) or lipopolysaccaride stimulation, ICAM-1 surface expression increased to a greater extent on brain than on umbilical vein endothelial cells. Dexamethasone at doses up to 100 microM had no effect on inhibiting cytokine-mediated upregulation of ICAM-1 on human brain microvascular endothelial cells.
authors.affiliation: Neurology Service, Veterans Affairs Medical Center, Augusta, GA 30904.
authors.forename: D C
authors.initials: DC
authors.lastname: Hess
authors.forename: T
authors.initials: T
authors.lastname: Bhutwala
authors.forename: J C
authors.initials: JC
authors.lastname: Sheppard
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.forename: J
authors.initials: J
authors.lastname: Smith
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis*; D002560:Cerebrovascular Circulation; D002561:Cerebrovascular Disorders / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008833:Microcirculation; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins
chemical_list: D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1