Kidney tubule enzymes and extracellular DNA in urine as markers for nephrotoxicity in the guinea pig.
Guinea pigs were given a single intraperitoneal injection of 1.35 mg/kg body weight of mercuric chloride; then various kidney enzymes and extracellular DNA were assayed in urine. Dramatic increases of all studied markers were observed on the first day following treatment. Sequential collection of urines allowed for kinetic studies: membrane markers alkaline phosphatase and gamma-glutamyltransferase were first released, then cytosolic lactate dehydrogenase and mitochondrial glutamate dehydrogenase, finally extracellular DNA; DNA release is equated with cell death. The features of kidney damage revealed by comparative and quantitative studies of these noninvasive markers suggest that brush border erasure was more extensive than cell necrosis.
Bret
L
L
Département de Physiopathologie, Ecole Nationale Vétérinaire, Toulouse, France.
Hasim
M
M
Lefebvre
H
H
Fournié
G J
GJ
Braun
J P
JP
D000469:Alkaline Phosphatase / Q000652:urine; D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000652:urine; D016923:Cell Death; D004247:DNA / Q000652:urine; D004798:Enzymes / Q000652:urine*; D005969:Glutamate Dehydrogenase / Q000097:blood; D006168:Guinea Pigs; D007684:Kidney Tubules / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008627:Mercuric Chloride / Q000633:toxicity*; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000097:blood
D015415:Biomarkers; D004798:Enzymes; D008627:Mercuric Chloride; D004247:DNA; D007770:L-Lactate Dehydrogenase; D005969:Glutamate Dehydrogenase; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase