Restriction enzyme fragment length polymorphisms of amplified herpes simplex virus type-1 DNA provide epidemiologic information.
Human herpes simplex type 1 (HSV-1) DNA of isolates from patients in a large teaching hospital was amplified by the polymerase chain reaction (PCR). The PCR products targeted approximately 2100 nt regions of relatively low G + C content. Comparison of restriction enzyme digests of amplified DNA showed variation useful for strain differentiation. Twelve nonrelated HSV-1 were differentiated from one another. In contrast, specimens epidemiologically related in an outbreak were indistinguishable from each other. Restriction endonuclease analysis of amplified HSV-1 sequences appears to be useful for molecular epidemiology and laboratory quality control to detect possible contamination by PCR products.
Haugen
T H
TH
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242.
Alden
B
B
Matthey
S
S
Nicholson
D
D
D001483:Base Sequence; D003596:Cytosine / Q000032:analysis; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D014644:Genetic Variation; D006147:Guanine / Q000032:analysis; D006561:Herpes Simplex / Q000453:epidemiology* / Q000382:microbiology; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D007484:Iowa / Q000453:epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D017931:DNA Primers; D004279:DNA, Viral; D006147:Guanine; D003596:Cytosine