title: Generation and molecular analyses of two rheumatoid synovial fluid-derived IgG rheumatoid factors.
abstract: OBJECTIVE
  To study the Ig genes that encode IgG rheumatoid factor (IgG-RF) from rheumatoid synovial fluid.
  
  
  METHODS
  We used rheumatoid synovial fluid B cells to generate IgG-RF-secreting hybridomas. We then characterized their binding properties and determined their nucleotide sequences.
  
  
  RESULTS
  Two monospecific IgG-RFs were obtained. Sequence analysis of the RFs revealed a new V lambda gene family (designated V lambda 9) and extensive somatic diversification, including a duplication-insertion of 18 nucleotides (6 amino acid residues) into a hypervariable region.
  
  
  CONCLUSIONS
  The data provide further support for an antigen-driven response in the sustained production of potentially pathogenic IgG-RFs in rheumatoid synovium.
authors.affiliation: Department of Medicine, University of California, San Diego.
authors.forename: E W
authors.initials: EW
authors.lastname: Lu
authors.forename: M
authors.initials: M
authors.lastname: Deftos
authors.forename: T
authors.initials: T
authors.lastname: Olee
authors.forename: D F
authors.initials: DF
authors.lastname: Huang
authors.forename: R W
authors.initials: RW
authors.lastname: Soto-Gil
authors.forename: D A
authors.initials: DA
authors.lastname: Carson
authors.forename: P P
authors.initials: PP
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001402:B-Lymphocytes / Q000378:metabolism; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012217:Rheumatoid Factor / Q000032:analysis* / Q000378:metabolism; D013582:Synovial Fluid / Q000276:immunology*
chemical_list: D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D004247:DNA; D012217:Rheumatoid Factor