Isolation of NotI sites from chromosome 22q11.
Chromosome 22q11 contains a large number of interesting loci, including genes associated with cancer and developmental defects. The region is also the site of the lambda immunoglobulin variable and constant regions and the BCR, gamma-glutamyl transpeptidase, and GGT-like activity multigene families. Because of the complexities associated with mapping highly related gene families, we have examined the utility of mapping large areas of DNA using a defined approach. A total of 21 complete NotI sites from band q11 were cloned and ordered into six noncontiguous clusters of sites using a combination of somatic cell hybrid panels, NotI jumping and linking libraries, and fluorescence in situ hybridization. The largest cluster spanned an estimated 2 Mb of NotI fragments, the smallest 115 kb. Approximately 3.5 Mb of band q11 could be examined for rearrangements in NotI restriction enzyme fragments. A number of conserved sequences, two genes, and a minimum of two families of related sequences were identified adjacent to NotI sites.
ten Hoeve
J
J
Department of Pathology, Childrens Hospital of Los Angeles, California 90027.
Morris
C
C
Poustka
A
A
Groffen
J
J
Heisterkamp
N
N
D000818:Animals; D002503:Centromere / Q000648:ultrastructure; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D006224:Cricetinae; D015252:Deoxyribonucleases, Type II Site-Specific; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D005810:Multigene Family; D009857:Oncogenes; D015183:Restriction Mapping; D005723:gamma-Glutamyltransferase / Q000235:genetics
D005819:Genetic Markers; D007127:Immunoglobulin Constant Regions; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D005723:gamma-Glutamyltransferase; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases