Development of a rapid quantitative assay for HIV-1 plasma infectious viraemia-culture-PCR (CPID).
A simple and rapid assay for the quantification of infectious HIV-1 in plasma was developed using short-term culture and DNA PCR. This method, called culture PCR, allows detection and quantification of infectious HIV-1 viraemia within 48 hours, and measures the number of infectious cell-free HIV-1 particles, expressed as culture PCR infectious doses (CPID/ml). 42 HIV infected subjects were assessed by this method. The titres obtained by CPID closely correlated with CD4+ count and clinical status. CPID titres had significant correlation with infectious virus titre determined by conventional limiting dilution tissue-culture methods. This culture-PCR technique permits rapid assessment of infectious plasma viraemia, and is comparable to longer culture based assay methods.
Ariyoshi
K
K
Department of GU and Communicable Diseases, St Mary's Hospital Medical School, London, England.
Bloor
S
S
Bieniasz
P D
PD
Bourrelly
M
M
Foxall
R
R
Weber
J N
JN
D000386:AIDS-Related Complex / Q000097:blood / Q000175:diagnosis*; D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000175:diagnosis*; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D017931:DNA Primers; D004279:DNA, Viral / Q000097:blood* / Q000302:isolation & purification; D016655:HIV Core Protein p24 / Q000032:analysis; D006679:HIV Seropositivity / Q000097:blood / Q000175:diagnosis*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000166:cytology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D014766:Viremia / Q000097:blood / Q000175:diagnosis*
D017931:DNA Primers; D004279:DNA, Viral; D016655:HIV Core Protein p24