Cell proliferation kinetics in acetic acid-induced gastric ulcer evaluated by immunohistochemical staining of proliferating cell nuclear antigen.
A monoclonal antibody to proliferating cell nuclear antigen (PCNA) has been previously shown to be capable of identifying proliferating cells. We investigated proliferative activity in the healing process of acetic acid-induced gastric ulcer by immunohistochemical staining of PCNA and 5-bromo-2-deoxyuridine (BrdU), and the two methods were compared. Cell proliferative activity of regenerated mucosa around ulcers showed continuous acceleration for 42 days, and PCNA-labeled cells had stained nuclei as clearly discernible as those of BrdU-labeled cells. In addition, immunohistochemical staining of PCNA provided reproducible and quantifiable results without the requirement of pretreatment. We conclude that immunohistochemical staining of PCNA may represent a useful technique for analysis of proliferative activity during healing of gastric ulcers.
Kitajima
T
T
Third Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Okuhira
M
M
Tani
K
K
Nakano
T
T
Hiramatsu
A
A
Mizuno
T
T
Inoue
K
K
D000085:Acetates / Q000494:pharmacology*; D019342:Acetic Acid; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D007124:Immunoenzyme Techniques; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000473:pathology
D000085:Acetates; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D019342:Acetic Acid