Effects of CD45 on NF-kappa B. Implications for replication of HIV-1.
Increased levels of replication of the HIV type 1 are observed after the activation of infected T cells through the TCR. However, anti-CD45 antibodies inhibit these effects in cells from infected individuals. In this study, we examined interrelationships between CD45 and HIV-1 further. We measured effects on the HIV-1 LTR in T cell lines that were stimulated with antibodies against CD45 and in those that lacked the expression of CD45 on their surfaces. First, anti-CD45 antibodies did not affect basal but decreased activated levels of expression from the HIV-1 LTR. Second, T cells, which lack CD45 and cannot signal via the TCR, supported higher levels of viral replication and gene expression. This was due to the presence of active NF-kappa B complexes in the nucleus of CD45- T cells. Additionally, infected T cells displayed lower levels of CD45 on their surfaces. Thus, CD45 plays an active role in the physiology of T cells and in the replication of HIV-1.
Baur
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A
Howard Hughes Medical Institute, University of California at San Francisco 94143-0724.
Garber
S
S
Peterlin
B M
BM
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000382:microbiology*; D002467:Cell Nucleus / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D006678:HIV / Q000254:growth & development*; D015658:HIV Infections / Q000276:immunology*; D016325:HIV Long Terminal Repeat; D006801:Humans; D066298:In Vitro Techniques; D017493:Leukocyte Common Antigens / Q000502:physiology*; D016328:NF-kappa B / Q000502:physiology*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D016328:NF-kappa B; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D017493:Leukocyte Common Antigens; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1