title: The rat preprotachykinin-A promoter is regulated in PC12 cells by the synergistic action of multiple stimuli.
abstract: We demonstrate in PC12 cells that although nerve growth factor, forskolin or potassium-evoked depolarisation independently induced minimal or no expression from the rat preprotachykinin-A gene (rPPT) promoter linked to a reporter gene, exposure of the cells to various combinations of these stimuli specifically activated the rPPT promoter in transient transfection assays.
authors.affiliation: MRC Brain Metabolism Unit, Royal Edinburgh Hospital, UK.
authors.forename: C F
authors.initials: CF
authors.lastname: Morrison
authors.forename: J
authors.initials: J
authors.lastname: McAllister
authors.forename: V
authors.initials: V
authors.lastname: Lyons
authors.forename: K
authors.initials: K
authors.lastname: Chapman
authors.forename: J P
authors.initials: JP
authors.lastname: Quinn
mesh_terms: D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D004338:Drug Combinations; D004594:Electrophysiology; D005786:Gene Expression Regulation / Q000187:drug effects; D009414:Nerve Growth Factors / Q000494:pharmacology; D016716:PC12 Cells; D011188:Potassium / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000235:genetics*; D051381:Rats / Q000235:genetics*; D013268:Stimulation, Chemical; D015320:Tachykinins / Q000235:genetics*
chemical_list: D004338:Drug Combinations; D009414:Nerve Growth Factors; D011498:Protein Precursors; D015320:Tachykinins; C044206:preprotachykinin; D005576:Colforsin; D011188:Potassium