[Erythrocyte preservative based on a solution of modified deionized gelatin with adenine and sodium phosphate].
The paper reports storage properties of a new erythro-preservative made of the solution of modified deionized gelatin with addition of adenine and disodium phosphate. 42-day storage of red cell suspension in the above preservative was assessed in comparison to Modejele preservative according to the following characteristics: free hemoglobin, the count of osmotically unstable red cells, red cell ATP concentration, red cell deformability index, zeta-potential, extracellular potassium and sodium concentrations, amount of microclots, acid-base condition of the suspensions, blood rheology. Control and test parameters showed the greatest difference by ATP concentrations: on storage day 42 ATP in the controls made up 58.9%, in the test series 77.8% against the baseline. Red cell deformability under storage in the new preservative also tended to a decrease. The rest test parameters did not differ much in the control and test series. Acute mouse, chronic rat, rabbit and dog experiments proved low toxicity of the preservative. The repeated introduction of the preparation brought about a slight shift to hypercoagulation.
Mel'nikova
V N
VN
Pleshakov
V T
VT
Selivanov
E A
EA
Degtereva
I N
IN
Kochetygov
N I
NI
Gerbut
K A
KA
Gorkun
A V
AV
Krivtsova
I M
IM
Rugal'
V I
VI
D000225:Adenine; D001793:Blood Preservation / Q000379:methods*; D004912:Erythrocytes / Q000187:drug effects*; D005780:Gelatin; D006801:Humans; D007477:Ions; D010710:Phosphates; D012996:Solutions
D007477:Ions; D010710:Phosphates; D012996:Solutions; D005780:Gelatin; D000225:Adenine; C018279:sodium phosphate
Konservant dlia éritrotsitov na osnove rastvora modifitsirovannogo deioinizirovannogo zhelatina s adeninom i natriia fosfatom.