A novel HLA haplotype containing a DRB5 gene associated with the DRB1*0103 allele.
A new hybrid haplotype, a DRB1*0103 allele associated with a DRB5*0101 allele, was found in a French Caucasoid family and has been described here. When these cells were typed by serology, contrarily to cells with the DR1 + 2s haplotype, they did not seem to be triplets. The reactivity of these cells with the DR2 allosera led to a false serological HLA-DR typing. RFLP analysis and DNA oligotyping after DR1-DRB1, DR2-DRB1 and DRB5 group-specific amplifications showed that there was no DR2-DRB1 product in these cells and demonstrated the segregation of a DR103 DR51 haplotype in the family.
Perrier
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P
Laboratoire d'Histocompatibilité, Centre Régional de Transfusion Sanguine, Vandoeuvre les Nancy, France.
Leotard
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B
Henry
M A
MA
D000483:Alleles; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D059847:HLA-DRB5 Chains; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D044465:White People / Q000235:genetics
D006680:HLA Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D059847:HLA-DRB5 Chains; D000949:Histocompatibility Antigens Class II