title: Crystallographic and cryo EM analysis of virion-receptor interactions.
abstract: Cryoelectron microscopy has been used to determine the first structure of a virus when complexed with its glycoprotein cellular receptor. Human rhinovirus 16 (HRV16) complexed with the two amino-terminal, immunoglobulin-like domains of the intercellular adhesion molecule-1 (ICAM-1) shows that ICAM-1 binds into the 12 A deep "canyon" on the surface of the virus. This is consistent with the prediction that the viral receptor attachment site lies in a cavity inaccessible to the host's antibodies. The atomic structures of HRV14 and CD4, homologous to HRV16 and ICAM-1, showed excellent correspondence with observed density, thus establishing the virus-receptor interactions.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana.
authors.forename: M G
authors.initials: MG
authors.lastname: Rossmann
authors.forename: N H
authors.initials: NH
authors.lastname: Olson
authors.forename: P R
authors.initials: PR
authors.lastname: Kolatkar
authors.forename: M A
authors.initials: MA
authors.lastname: Oliveira
authors.forename: R H
authors.initials: RH
authors.lastname: Cheng
authors.forename: J M
authors.initials: JM
authors.lastname: Greve
authors.forename: A
authors.initials: A
authors.lastname: McClelland
authors.forename: T S
authors.initials: TS
authors.lastname: Baker
mesh_terms: D015815:Cell Adhesion Molecules / Q000648:ultrastructure*; D015925:Cryopreservation; D018360:Crystallography, X-Ray; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008958:Models, Molecular; D008961:Models, Structural; D011991:Receptors, Virus / Q000648:ultrastructure*; D012229:Rhinovirus / Q000648:ultrastructure*; D013329:Structure-Activity Relationship; D014771:Virion / Q000648:ultrastructure*
chemical_list: D015815:Cell Adhesion Molecules; D046911:Macromolecular Substances; D011991:Receptors, Virus; D018799:Intercellular Adhesion Molecule-1