title: Bovine leukocyte adhesion deficiency in Danish Holstein-Friesian cattle. I. PCR screening and allele frequency estimation.
abstract: A screening program for bovine leukocyte adhesion deficiency (BLAD) in Danish Holstein-Friesian cattle has been initiated. During the first months 1611 animals were tested by a PCR based assay. Of these animals 1256, 346, and 8 were assigned normal, BLAD carriers, and BLAD affected animals, respectively. One bull, born as a co-twin, showed weak reaction for the BLAD allele on DNA isolated from leukocytes, but a normal genotype on DNA isolated from semen. Chromosome analysis showed that this bull was a blood chimaera. Estimation of the BLAD allele frequency upon the PCR test results showed that around 450 Danish calves born in 1991 might have been affected with the recessive disorder.
authors.affiliation: Department of Animal Science and Animal Health, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
authors.forename: C B
authors.initials: CB
authors.lastname: Jørgensen
authors.forename: J S
authors.initials: JS
authors.lastname: Agerholm
authors.forename: J
authors.initials: J
authors.lastname: Pedersen
authors.forename: P D
authors.initials: PD
authors.lastname: Thomsen
mesh_terms: D000483:Alleles; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D001483:Base Sequence; D018845:CD11 Antigens; D018821:CD18 Antigens; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D003718:Denmark; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005260:Female; D005787:Gene Frequency / Q000235:genetics*; D005820:Genetic Testing / Q000662:veterinary; D005838:Genotype; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000662:veterinary*; D018370:Leukocyte-Adhesion Deficiency Syndrome; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D016028:Receptors, Leukocyte-Adhesion / Q000235:genetics
chemical_list: D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; D017931:DNA Primers; D016028:Receptors, Leukocyte-Adhesion; D004247:DNA