Hypothermic preservation of hepatocytes on gelatin gels.
Hypothermic preservation of hepatocytes on gelatin gels allows hepatocytes to be stored for at least 9 days. The procedure is easy, inexpensive and does not require specialised equipment. The cells retain their morphology and are released as separate spherical entities by dissolving the gelatin layer at 37 degrees. The recovered cells have an intact plasma membrane as judged by lactate dehydrogenase activity, attachment efficiency and subsequent monolayer formation in culture. Functional tests show that the cells recover quickly from the storage conditions. Rates of protein synthesis are maintained over a 6 day period and remain at 62% of the initial level on day 9. The liver specific, hormonal induction of the enzyme tyrosine aminotransferase is apparent throughout the culture period. In addition a phenotypic marker enzyme, gamma-glutamyl transpeptidase, remained at basal levels.
Evans
P J
PJ
School of Pure and Applied Biology, University of Wales College of Cardiff.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002448:Cell Adhesion; D015925:Cryopreservation / Q000379:methods*; D003907:Dexamethasone / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005780:Gelatin; D005782:Gels; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D014176:Protein Biosynthesis; D014444:Tyrosine Transaminase / Q000096:biosynthesis; D005723:gamma-Glutamyltransferase / Q000096:biosynthesis
D005782:Gels; D003994:Bucladesine; D003907:Dexamethasone; D005780:Gelatin; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D014444:Tyrosine Transaminase