title: Effect of preservatives on systemic delivery of insulin by ocular instillation in rabbits.
abstract: The effects of absorption promoters and ophthalmic preservatives on the systemic absorption of insulin through the ocular route were investigated in albino rabbits. Insulin absorption was evaluated by its hypoglycaemic response. Although ocular instillation of insulin alone did not decrease the serum glucose concentration, instillation of insulin with absorption promoters such as EDTA and saponin decreased it. The promoting effect depended on the concentration of the absorption promoters. Of ophthalmic preservatives investigated (benzalkonium chloride, paraben, 2-phenylethanol, benzyl alcohol and sorbic acid), benzalkonium, chloride and paraben showed promoting effects. The promoting effect for benzalkonium chloride was reversible and dependent on concentration of both benzalkonium chloride and insulin in the formulation.
authors.affiliation: School of Pharmaceutical Sciences, Nagasaki University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: C
authors.initials: C
authors.lastname: Tei
authors.forename: K
authors.initials: K
authors.lastname: Yamamura
authors.forename: K
authors.initials: K
authors.lastname: Nishida
authors.forename: J
authors.initials: J
authors.lastname: Nakamura
mesh_terms: D000042:Absorption; D000818:Animals; D001548:Benzalkonium Compounds / Q000008:administration & dosage / Q000494:pharmacology; D001682:Biological Availability; D001786:Blood Glucose / Q000378:metabolism; D005123:Eye / Q000378:metabolism*; D007328:Insulin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D008297:Male; D009883:Ophthalmic Solutions; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology*; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001548:Benzalkonium Compounds; D001786:Blood Glucose; D007328:Insulin; D009883:Ophthalmic Solutions; D011310:Preservatives, Pharmaceutical