title: Proliferating cell nuclear antigen in archival surgical specimens of malignant lymphoma and metastatic carcinoma: immunohistochemical and flow cytometric analysis.
abstract: Immunohistochemical and flow cytometric multiparameter analysis of proliferating cell nuclear antigen (PCNA) was performed on fifteen formalin fixed, paraffin embedded lymph nodes with malignant lymphoma (eleven non-Hodgkin's lymphomas, four Hodgkin's lymphomas), and fifteen lymph nodes with metastatic carcinomas. A general concordance between PCNA measurement by both methods has been observed: the percentage of positively stained cells in tissue sections correlated well with the percentage of cells expressing this antigen in cell suspensions (r = 0.76). Both diploid and aneuploid tumors expressed PCNA, and a correlation between PCNA and the percent cells in S-phase was evident in both: in PCNA-positive tumors the mean percent of cells in S-phase was 16.5%, and in PCNA-negative tumors, 5.9%. The data indicate that PCNA can be detected in formalin-fixed tissues by either classic immunohistochemical analysis or by flow cytometry.
authors.affiliation: Cancer Research Institute, New York Medical College, Valhalla.
authors.forename: W
authors.initials: W
authors.lastname: Gorczyca
authors.forename: A
authors.initials: A
authors.lastname: Kram
authors.forename: T
authors.initials: T
authors.lastname: Tuziak
authors.forename: Z
authors.initials: Z
authors.lastname: Darzynkiewicz
authors.forename: M R
authors.initials: MR
authors.lastname: Melamed
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D002277:Carcinoma / Q000276:immunology* / Q000556:secondary; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007822:Laryngeal Neoplasms / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000276:immunology*; D009687:Nuclear Proteins / Q000032:analysis*; D011003:Ploidies; D018809:Proliferating Cell Nuclear Antigen; D016196:S Phase / Q000502:physiology
chemical_list: D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen