title: Lymphocyte adhesion to brain capillary endothelial cells in vitro.
abstract: The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1 beta (rhIL-1 beta), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries. Adhesion of lymphocytes to BBEC increased 4.1-fold after stimulation of the endothelial cells for 4 h with 5 or 10 ng/ml LPS. Lymphocyte adhesion increased after incubation of the BBEC for 4 h with IL-1 and was increased 3.7-fold using 100 ng/ml IL-1. BBEC pre-incubated with IL-6 for 4 h also showed an increase in adhesion of lymphocytes, and cells pretreated with 100 ng/ml IL-6 showed a 3-fold increase in lymphocyte adherence. Specific monoclonal antibodies directed against CD11a, CD18, and VLA-4 were able to block adherence of lymphocytes to stimulated BBEC. These results indicate that the in vitro activation of BBEC may serve as a model for the study of inflammation of the blood-brain barrier.
authors.affiliation: Division of Pharmacology, Leiden/Amsterdam Center for Drug Research, University of Leiden, Sylvius Laboratories, The Netherlands.
authors.forename: H E
authors.initials: HE
authors.lastname: de Vries
authors.forename: A C
authors.initials: AC
authors.lastname: Moor
authors.forename: M C
authors.initials: MC
authors.lastname: Blom-Roosemalen
authors.forename: A G
authors.initials: AG
authors.lastname: de Boer
authors.forename: D D
authors.initials: DD
authors.lastname: Breimer
authors.forename: T J
authors.initials: TJ
authors.lastname: van Berkel
authors.forename: J
authors.initials: J
authors.lastname: Kuiper
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000502:physiology; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply*; D018821:CD18 Antigens; D002196:Capillaries / Q000166:cytology; D002417:Cattle; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1; D008070:Lipopolysaccharides / Q000494:pharmacology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology*; D016029:Receptors, Very Late Antigen / Q000502:physiology
chemical_list: D015703:Antigens, CD; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D008070:Lipopolysaccharides; D016169:Lymphocyte Function-Associated Antigen-1; D016029:Receptors, Very Late Antigen; D018799:Intercellular Adhesion Molecule-1