Determination of chromosome size and number of rrn loci in Lactobacillus plantarum by pulsed-field gel electrophoresis.
The size of the Lactobacillus plantarum CCM 1904 chromosome was determined by pulse-field gel electrophoresis. It was found to be 3.3-3.4 Mb using SfiI or AscI restriction endonucleases, compared to 3-4 Mb found for the other L. plantarum strains tested. L. plantarum CCM 1904 5S rDNA was clonedl by polymerase chain reaction, sequenced, and used as a probe to characterize strains. At least five rrn loci were found. The pulsed-field gel electrophoresis macrorestriction patterns were strain-specific, while the rDNA restriction hybridization patterns were species-specific.
Chevallier
B
B
Laboratoire de Microbiologie et de Génétique de l'Université Louis-Pasteur, Strasbourg, France.
Hubert
J C
JC
Kammerer
B
B
D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D007778:Lactobacillus / Q000235:genetics*; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal