title: Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autolysin operon of Bacillus subtilis.
abstract: Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene constructed in the B. subtilis chromosome. The phosphorylated form of the DegU protein seems to be a regulator for expression of the sigD gene.
authors.affiliation: Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tokunaga
authors.forename: M H
authors.initials: MH
authors.lastname: Rashid
authors.forename: A
authors.initials: A
authors.lastname: Kuroda
authors.forename: J
authors.initials: J
authors.lastname: Sekiguchi
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics*; D009876:Operon; D011494:Protein Kinases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C066496:DegS protein, Bacteria; C066497:DegU protein, Bacteria; D012333:RNA, Messenger; D012808:Sigma Factor; D011494:Protein Kinases; D009238:N-Acetylmuramoyl-L-alanine Amidase