Mitochondrial free Ca2+ concentration in living cells.
Evidence has accrued during the past two decades that mitochondrial Ca2+ plays an important role in the regulation of numerous cell functions such as energy metabolism. This implies that mitochondrial Ca2+ transport systems might be able to relay the changes of cytosolic Ca2+ concentration ([Ca2+]c) into mitochondrial matrix for regulating biochemical activities. To substantiate this idea, measurements of intramitochondrial free Ca2+ concentration ([Ca2+]m) become essential. In this article, we review the results from recent studies attempting to measure [Ca2+]m in living cells. In addition, the significance of each study is discussed.
Sheu
S S
SS
Department of Pharmacology, University of Rochester, School of Medicine and Dentistry, New York 14642.
Jou
M J
MJ
D000331:Aequorin; D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000378:metabolism*; D002460:Cell Line; D007668:Kidney; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D000331:Aequorin; D002118:Calcium