Postanoxic formation of aldehydic lipid peroxidation products in human renal tubular cells.
The levels of the aldehydic lipid peroxidation products 4-hydroxynonenal and malondialdehyde and the levels of adenine nucleotides were measured during anoxia/reoxygenation studies with isolated human renal tubular cells in vitro. Energy depletion of renal cells was demonstrated by the decrease of ATP level. ATP could be restored completely and rapidly during postanoxic reoxygenation. 4-Hydroxynonenal and malondialdehyde levels increased during reoxygenation. In parallel, the breakdown of physiological 4-hydroxynonenal levels was estimated. The 4-hydroxynonenal formation rate was estimated from accumulation and metabolic breakdown rates of this compound.
Grune
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Clinic of Physical Medicine, Medical Faculty (Charité), Humboldt University Berlin, Germany.
Sommerburg
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O
Petras
T
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Siems
W G
WG
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism*; D002292:Carcinoma, Renal Cell; D015687:Cell Hypoxia; D002851:Chromatography, High Pressure Liquid; D005609:Free Radicals; D006801:Humans; D007680:Kidney Neoplasms; D007684:Kidney Tubules / Q000378:metabolism*; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D010100:Oxygen / Q000008:administration & dosage
D000447:Aldehydes; D005609:Free Radicals; D008315:Malondialdehyde; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C027576:4-hydroxy-2-nonenal; D010100:Oxygen