[Diagnosis of tuberculosis with polymerase chain reaction].
A new simple method for the detection of pathogenic species Mycobacteriae in clinical samples by use of polymerase chain reaction (PCR) was described. This method is high sensitive, specific and fast. One pair of primers were synthetized for use in PCR with DNA-extracts and whole cells lysates from patient's sputum. Only strains belonging to species of the M. tuberculosis contained an amplifiable fragment of 240 base pairs. Using PCR we achieved the increasing of identification value pathogenic M. tuberculosis in sputum from 44.1% (Ziehl-Nielsen staining) and 55.9% (culturing) to 73.5%.
Ziraweckij
M I
MI
Lwowskiego Instytutu Naukowo-Badawczego Epidemiologii i Higieny.
Borys
W M
WM
Gładarewska
N M
NM
Kostyk
O P
OP
Jukalo
W E
WE
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D004269:DNA, Bacterial
Rozpoznanie zakazenia gruźlica za pomoca polimerazowej reakcji łańcuchowej.