title: Analysis of lower aliphatic aldehydes in water by micellar electrokinetic chromatography with derivatization to 2,4-dinitrophenylhydrazones.
abstract: The analysis of lower aliphatic aldehydes in water following derivatization to 2,4-dinitrophenylhydrazones, was investigated by micellar electrokinetic chromatography (MEKC). The effects of the buffer pH and acetonitrile addition on the separation were investigated. Under optimized conditions the 2,4-dinitrophenylhydrazine (DNPH) reagent was completely separated from four DNPH derivatized aldehydes with high separation efficiency. DNPH-formaldehyde and DNPH-acetaldehyde were also completely separated from one another. Their relative standard deviation of migration times and peak areas were within 1.0% and 3.3%, respectively. The reproducibility of peak areas in this MEKC analysis was as good as that of high-performance liquid chromatography (HPLC) analysis with the same derivatization method. The recovery values from tap and river water were in the range of 97-102%. The detection limits of formaldehyde and acetaldehyde were 0.05 mg/L and 0.08 mg/L respectively.
authors.affiliation: Osaka National Research Institute, AIST, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takeda
authors.forename: S
authors.initials: S
authors.lastname: Wakida
authors.forename: M
authors.initials: M
authors.lastname: Yamane
authors.forename: K
authors.initials: K
authors.lastname: Higashi
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000032:analysis; D000171:Acrolein / Q000031:analogs & derivatives / Q000032:analysis; D000447:Aldehydes / Q000032:analysis* / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004586:Electrophoresis / Q000379:methods*; D005557:Formaldehyde / Q000031:analogs & derivatives / Q000032:analysis; D006835:Hydrazones / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D008823:Micelles; D014867:Water
chemical_list: C001072:2,4-dinitrophenylhydrazone; D000447:Aldehydes; D006835:Hydrazones; D008823:Micelles; D014867:Water; D005557:Formaldehyde; D000171:Acrolein; C005556:propionaldehyde; D000079:Acetaldehyde