Regulation of adrenergic receptors in the rat kidney.
Chronic daily application of (+/-)-isoprenaline induced a selective-down regulation of beta-adrenoceptors in the kidney: the concentration of [3H]dihydroalprenolol binding sites was significantly lowered by isoprenaline treatment while [3H]prazosin and [3H]rauwolscine binding, representing alpha 1- and alpha 2-adrenoceptors, respectively, was not markedly altered. Since the proportion of high- and low-affinity sites for the non-selective alpha- but relatively beta 1-selective agonist (-)-noradrenaline remained constant and since in [3H]dihydroalprenolol competition experiments the high- and low-affinity site ratio fitted well to the beta 1/beta 2 relation, determined independently by employing ICI 118551 as a beta 2-selective ligand, a parallel decrease of both beta 1- and beta 2-adrenoceptor density can be concluded.
Lübbecke
F
F
Center of Internal Medicine (Medizinische Klinik II), Justus-Liebig-University, Giessen, Germany.
Schneider
D
D
Schütterle
G
G
D000818:Animals; D001667:Binding, Competitive; D001835:Body Weight / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology; D007668:Kidney / Q000502:physiology* / Q000648:ultrastructure*; D007700:Kinetics; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D011941:Receptors, Adrenergic / Q000032:analysis / Q000187:drug effects / Q000502:physiology*
D011941:Receptors, Adrenergic; D007545:Isoproterenol