[Construction of human glioma cDNA library].
Total RNA from a human glioma was extracted by acid guanidinium-thiocyanate-chloroform one step method, and poly(A+)RNA was separated by affinity chromatography on oligo (dT) cellulose. The cDNA was synthesized by using poly (A+) RNA as template, the length of cDNA being 0.2-5 kb. The glioma cDNA inserts could be cloned into lambda gt11 vector, the resulting recombinant DNA was packaged in vitro. The cDNA library of 1.12 x 10(5) pfu/ml was obtained, and the cloning efficiency was 4.8 x 10(3)/ng cDNA. This library might contribute an important basis to study of the structure and function of human glioma genes.
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D001932:Brain Neoplasms / Q000235:genetics*; D018076:DNA, Complementary / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000096:biosynthesis; D015723:Gene Library; D005910:Glioma / Q000235:genetics*; D006801:Humans; D012334:RNA, Neoplasm / Q000302:isolation & purification
D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm