Structure of the mouse arylsulfatase A gene and cDNA.
The murine arylsulfatase A (ARSA) gene and cDNA have been cloned and sequenced. The gene is 3.8 kb long and contains eight exons. All intron/exon splice junctions conform to the GT/AG consensus sequence. The genomic structure is similar to that of the human gene. One major RNA species of 3.2 kb is transcribed. This RNA species has a 5' untranslated region of 638 nucleotides and terminates in a region around nucleotide 700 downstream of the termination codon. In addition, a rare mRNA species terminating at a polyadenylation signal 135 nucleotides downstream of the termination codon has been found. A larger transcript of 4 kb can be detected in liver. The size difference is due to initiation of transcription 5' of the cap site of the 3.2-kb mRNA species. The entire ARSA cDNA has been cloned by PCR from reverse-transcribed RNA. The coding sequence has 1518 nucleotides and predicts a protein of 506 amino acids. The nucleotide as well as the amino acid sequence is highly conserved among humans and mice.
Kreysing
J
J
Department of Biochemistry II, Georg August Universität Göttingen, Federal Republic of Germany.
Polten
A
A
Hess
B
B
von Figura
K
K
Menz
K
K
Steiner
F
F
Gieselmann
V
V
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002553:Cerebroside-Sulfatase / Q000235:genetics*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D006801:Humans; D007966:Leukodystrophy, Metachromatic / Q000235:genetics; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014158:Transcription, Genetic
D018076:DNA, Complementary; D012333:RNA, Messenger; D002553:Cerebroside-Sulfatase