Nine polymorphic markers characterized by polymerase chain reaction techniques form two linkage groups on rat chromosome 8.
Five genes and four anonymous polymorphic markers, forming two linkage groups, were mapped in F2 intercross progeny of F344/N x LEW/N rats using polymerase chain reaction (PCR) techniques. Both linkage groups were assigned to rat chromosome 8 because they contained genetic loci previously mapped to this chromosome. The first group was comprised of markers for three anonymous loci and two gene loci, thymus cell antigen-1 (Thy1) and tropoelastin (Eln). The second group was comprised of markers for one anonymous locus and three gene loci, cellular retinol binding protein II (Rbp2), matrin F/G (Matr1), and acyl-peptide hydrolase (Apeh). Seven markers (identified by simple sequence repeat associated length polymorphisms) were characterized in an additional 13 inbred rat strains (ACI/N, BN/SsN, BUF/N, LER/N, LOU/MN, MNR/N, MR/N, SHR/N, SR/Jr, SS/Jr, WBB1/N, WBB2/N, and WKY/N). Two to six alleles were detected for each marker. The reported markers should facilitate genetic mapping and monitoring of inbred rat strains.
Mathern
P
P
Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.
Goldmuntz
E A
EA
Du
Y
Y
Zha
H
H
Cash
J M
JM
Crofford
L J
LJ
Wilder
R L
RL
Remmers
E F
EF
D000483:Alleles; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017931:DNA Primers; D008040:Genetic Linkage; D005819:Genetic Markers; D020783:Matrix Metalloproteinase 7; D008562:Membrane Glycoproteins / Q000235:genetics; D008666:Metalloendopeptidases / Q000235:genetics; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012177:Retinol-Binding Proteins / Q000235:genetics; D054840:Retinol-Binding Proteins, Cellular; D013045:Species Specificity; D018800:Thy-1 Antigens; D014333:Tropoelastin / Q000235:genetics
D000954:Antigens, Surface; D017931:DNA Primers; D005819:Genetic Markers; D008562:Membrane Glycoproteins; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D018800:Thy-1 Antigens; D014333:Tropoelastin; D010447:Peptide Hydrolases; D008666:Metalloendopeptidases; D020783:Matrix Metalloproteinase 7