Oscillatory variations in the amount of protein extractable from murine erythroleukemia cells: stimulation by insulin.
The amount of protein extractable from murine erythroleukemia (MEL) cells varies in an oscillatory manner at high frequency and high amplitude as it does for several other cell lines. Moreover, the rhythm appears to be modulated in periodic fashion with respect to the mean, period and amplitude. The phenomenon thus seems to be universal and fundamental. Time series analyses support the view that several periodicities contribute to the observed protein rhythm. Insulin affects the dynamics as it does for both morphological and phosphorylation oscillations. Caution is necessary in the interpretation of 'specific activities' of cellular components and in electrophoretic studies wherein equal amounts of protein are applied to the wells in an effort to correct for random errors.
Ferreira
G M
GM
Department of Medical Biochemistry, Medical School, University of the Witwatersrand, Parktown, Johannesburg, South Africa.
Hammond
K D
KD
Gilbert
D A
DA
D000818:Animals; D001683:Biological Clocks / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D007328:Insulin / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D015203:Reproducibility of Results; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D007328:Insulin; D009363:Neoplasm Proteins