Separation and characterization of the main methylated nucleobases from nuclear, cytoplasmic and poly (A)+ RNA by high-performance liquid chromatography and mass spectrometry.
We were able to detect nine methylated nucleobases (3-methyluracil, 1-, 2-, 3- and 7-methylguanine, 1-, 2-, 3- and 6-methyladenine) in RNA from rat and calf liver, baker's yeast, Torula and Euglena cells by using reversed-phase high-performance liquid chromatography and thermospray mass spectrometry. Total cellular, nuclear, cytoplasmic and poly (A)+ RNA from rat liver showed marked methylation, mainly of 1- and 3- methylguanine, and 3- and 2-methyladenine. These bases were especially abundant in nuclear RNA and, to a lesser extent, in poly (A)+ RNA. In contrast, 7-methylguanine and 6-methyladenine were poorly represented in poly (A)+ RNA.
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Laboratoire de Biochimie de la Différenciation, Institut Jacques Monod, CNRS-Université Paris VII, France.
Bertaux
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Valencia
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Becue
T
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Fournier
T
T
Biou
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Porquet
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D
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003454:Cryptococcus / Q000235:genetics; D003593:Cytoplasm / Q000737:chemistry; D005056:Euglena gracilis / Q000235:genetics; D013058:Mass Spectrometry / Q000379:methods*; D008745:Methylation; D009705:Nucleosides / Q000737:chemistry / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000737:chemistry*; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000235:genetics
D009705:Nucleosides; D012333:RNA, Messenger