A protease inhibitor of the serpin family is a major protein in carp perimeningeal fluid: I. Protein purification and characterization.
Two isoforms of a protease inhibitor of the serpin family (p62) have been purified from bighead carp perimeningeal fluid. Both isoforms migrate with an apparent molecular mass of 62 kDa on reducing and nonreducing sodium dodecyl sulfate-polyacrylamide gels. Both proteins inhibited the activities of bovine trypsin, bovine chymotrypsin, and porcine pancreatic elastase. They also formed complexes with these proteases that were resistant to sodium dodecyl sulfate treatment. p62 exists in the extracts of all tissues examined, including brain, head kidney, kidney, liver, muscle, ovary, pituitary, and spleen. It is also present in serum, ovarian fluid, and milt as well as perimeningeal fluid. The protease inhibitor is a glycoprotein, and its carbohydrate moiety could be removed by endoglycosidase F. Because p62 resembles mammalian alpha 1-antitrypsin in many aspects, it is likely a fish equivalent of alpha 1-antitrypsin.
Huang
C J
CJ
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
Chen
C C
CC
Chen
H J
HJ
Huang
F L
FL
Chang
G D
GD
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D002347:Carps / Q000378:metabolism*; D002417:Cattle; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008578:Meninges / Q000378:metabolism*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D011480:Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D015843:Serpins / Q000378:metabolism*; D013552:Swine; D014018:Tissue Distribution; D014361:Trypsin Inhibitors / Q000494:pharmacology
D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D011480:Protease Inhibitors; D015843:Serpins; D014361:Trypsin Inhibitors; D006026:Glycoside Hydrolases; D002918:Chymotrypsin; D010196:Pancreatic Elastase