A frequent deletion of chromosome 5q21 in advanced small cell and non-small cell carcinoma of the lung.
We have examined the deletion of the long arm of chromosome 5 (5q) in 59 cases of advanced lung cancer [39 cases of small cell lung cancer (SCLC), 20 cases of non-SCLC] using 12 restriction fragment length polymorphism markers on 5q. Of 59 lung cancer cases, 48 (81%) exhibited deletion at any portion of the 5q locus (loci). Such a high frequency of 5q deletion has not been reported in surgically resectable non-SCLC. One SCLC case showed a 5q deletion only in metastatic sites but not in the primary cancer. These data suggest that the inactivation of putative tumor-suppressor gene(s) on 5q may be a late event in the progression of lung cancer. There was no significant difference in frequency of 5q deletion between SCLC and non-SCLC. Compared to non-SCLC, however, SCLC usually showed widespread deletion on 5q. While the most frequent target region was estimated to be about 3-5 megabases at 5q21 around the adenomatous polyposis coli (APC) gene locus, some cases showed more telomeric deletion (5q33-35), suggesting that there are at least two different tumor-suppressor genes on 5q associated with the progression of lung cancer.
Hosoe
S
S
Department of Medicine III, Osaka University Medical School, Japan.
Ueno
K
K
Shigedo
Y
Y
Tachibana
I
I
Osaki
T
T
Kumagai
T
T
Tanio
Y
Y
Kawase
I
I
Nakamura
Y
Y
Kishimoto
T
T
D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D018288:Carcinoma, Small Cell / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004273:DNA, Neoplasm / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D017491:Genes, APC; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D012150:Polymorphism, Restriction Fragment Length
D004273:DNA, Neoplasm; D005819:Genetic Markers