Differential expression of protooncogenes in human germ cell tumors of the testis.
BACKGROUND
It has been suggested that tumorigenesis of the germ cell tumor of the testis includes abnormal and developmentlike differentiation of primordial germ cells to several mature type tumors.


METHODS
To clarify roles of protooncogenes in the unique tumorigenic mechanism in the human germ cell tumor, the authors examined the expression of 15 protooncogenes in human primary germ cell tumors of the testis with Northern blot analyses.


RESULTS
Fifteen (94%) of 16 seminomas and 5 (83%) of 6 embryonal carcinomas had a significant levels of N-myc expression, whereas they did not express two receptor type protooncogenes, c-erbB-1 and c-erbB-2. In contrast, some immature teratomas had a high level of c-erbB-1 expression, and an advanced case showed a significant level of c-erbB-2 expression. Immature teratomas did not show N-myc expression. Higher levels of c-mos expression were observed in several cases of seminomas and embryonal carcinomas. Expression of c-Ki-ras or N-ras was observed in all histologic subgroups and normal testes.


CONCLUSIONS
A significant level of N-myc expression may be essential for undifferentiated tumors including seminoma and embryonal carcinoma, whereas c-erbB-1 and possibly c-erbB-2 may have important roles in the differentiated tumors such as immature teratoma. These results suggest that some of the protooncogene expression may be switched critically during the differentiation from seminomas or embryonal carcinomas to the more differentiated-type tumor.
Shuin
T
T
Department of Urology, Yokohama City University School of Medicine, Japan.
Misaki
H
H
Kubota
Y
Y
Yao
M
M
Hosaka
M
M
D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D018236:Carcinoma, Embryonal / Q000235:genetics; D066246:ErbB Receptors / Q000032:analysis / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D016236:Genes, fms / Q000235:genetics; D016258:Genes, mos / Q000235:genetics; D016259:Genes, myc / Q000235:genetics; D011905:Genes, ras / Q000235:genetics; D018237:Germinoma / Q000235:genetics*; D006801:Humans; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics; D017231:Proto-Oncogene Proteins c-mos / Q000032:analysis / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis / Q000235:genetics; D019908:Proto-Oncogene Proteins c-raf; D011519:Proto-Oncogenes / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000235:genetics; D018239:Seminoma / Q000235:genetics; D013724:Teratoma / Q000235:genetics; D013736:Testicular Neoplasms / Q000235:genetics*; D013737:Testis / Q000737:chemistry
D014408:Biomarkers, Tumor; D066246:ErbB Receptors; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D017231:Proto-Oncogene Proteins c-mos; D016271:Proto-Oncogene Proteins c-myc; D019908:Proto-Oncogene Proteins c-raf; D018719:Erb-b2 Receptor Tyrosine Kinases; D016186:Receptor, Macrophage Colony-Stimulating Factor