Differential expression of PKC alpha and PKC beta isozymes in CD4+, CD8+ and CD4+/CD8+ double positive human T cells.
Using specific monoclonal and polyclonal antibodies, we have analyzed protein kinase C alpha and beta isozyme expression in human T cells from peripheral blood (PB) and from thymus. While the PKC beta isozyme was present in all T cell sub-types isolated from both PB and thymus, the alpha isozyme was found only in single positive CD4+ thymocytes, in PB-CD4+ lymphocytes and in PB-CD8+ T cells from several donors. It was absent from both, CD8+ and double positive CD4+/CD8+ thymocytes. These results show that PKC alpha and -beta are differentially regulated during intra-thymic development and suggest that PKC alpha plays a specific role in helper T cell function.
Tarantino
N
N
Laboratoire d'Immunologie Cellulaire et Tissulaire, CNRS URA 625, Hôpital de la Pitié Salpêtrière, Paris, France.
Debré
P
P
Körner
M
M
D015704:CD4 Antigens; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D016827:CD8 Antigens; D002478:Cells, Cultured; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis*; D011493:Protein Kinase C / Q000096:biosynthesis*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology
D015704:CD4 Antigens; D016827:CD8 Antigens; D007527:Isoenzymes; D011493:Protein Kinase C