On-line fully automated solid-phase extraction-liquid chromatography analysis of 1,2-dihydro-4-(1,2-dihydro-2-oxo-1-pyridyl)-2,2-dimethyl-1-oxonaphthale ne-6- carbonitrile (UR-8225), a new potassium channel opener, in plasma samples.
A fully automated reversed-phase HPLC method for pharmacokinetic studies was developed for the determination in plasma samples of 1,2-dihydro-4-(1,2-dihydro-2-oxo-1-pyridyl)-2,2-dimethyl-1-oxonaphtha lene-6- carbonitrile (compound I), a new potassium channel opener. On-line solid-phase extraction was performed with disposable C18 cartridges. After clean up, the samples were eluted and transferred onto an RP-18 analytical column, where separation was performed with a mobile phase of acetonitrile-10 mM di-n-butylamine phosphate (28:72, v/v). Ultraviolet absorbance detection was used at 236 nm. The detector response was linear in the range 25-2500 ng/ml, and the lowest limit of quantitation was determined at 2.5 ng/ml. The inter-day variability was < 4% for samples at 1000 ng/ml and < 15% for samples at 10 ng/ml. This method was used for the pharmacokinetic study of compound I in rats at three different dosage levels.
Nieto
C
C
Research Centre, J. Uriach and Cia, S.A., Barcelona, Spain.
Ramis
J
J
Conte
L
L
Fernández
J M
JM
Forn
J
J
D000818:Animals; D000959:Antihypertensive Agents / Q000097:blood* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009281:Naphthalenes / Q000097:blood* / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects*; D011728:Pyridones / Q000097:blood* / Q000494:pharmacology; D051381:Rats; D015203:Reproducibility of Results
D000959:Antihypertensive Agents; D009281:Naphthalenes; D015221:Potassium Channels; D011728:Pyridones; C082407:UR 8225