title: Properties of a new fibrin glue stable in liquid state.
abstract: A pasteurized preparation of fibrin glue composed of two separate stable, liquid components: highly purified human thrombin and fibrinogen concentrate is described. The components are mixed extemporaneously during application. Thrombin was prepared using a prothrombin complex concentrate as starting material which was activated by calcification and then heated in solution during 10 hours at 60 degrees C in the presence of stabilizers. The isolation of thrombin was carried out using a column of benzamidine-Sepharose 6B. The eluate contained thrombin with a high degree of purity (more than 95% assessed by SDS-PAGE) with a specific activity > 2,500 IU/mg protein. The purified liquid thrombin preparation remained stable for at least 6 months. The fibrinogen concentrate was prepared from cryoprecipitate after removal of factor VIII and then virally inactivated by pasteurization in the presence of glucose and sorbitol. After purification the concentrate containing a high level of fibrinogen was formulated with urea 0.5 M or arginine 5% before conditioning. Both components of the fibrin glue kept its biological properties for more than 6 months at +4 degrees C.
authors.affiliation: Centre National de Transfusion Sanguine, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Chabbat
authors.forename: M
authors.initials: M
authors.lastname: Tellier
authors.forename: P
authors.initials: P
authors.lastname: Porte
authors.forename: M
authors.initials: M
authors.lastname: Steinbuch
mesh_terms: D000268:Adhesiveness; D004586:Electrophoresis; D005079:Excipients / Q000737:chemistry; D015718:Fibrin Tissue Adhesive / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D013917:Thrombin / Q000737:chemistry
chemical_list: D005079:Excipients; D015718:Fibrin Tissue Adhesive; D005340:Fibrinogen; D013917:Thrombin