Sequencing of a tet(Q) gene isolated from Bacteroides fragilis 1126.
Recently, Tet Q, a tetracycline resistance determinant that confers resistance by a ribosome protection mechanism, was described and added to the two previously described classes, Tet M and Tet O. The first representative of this class, tetA(Q)1, was isolated from Bacteroides thetaiotaomicron DOT. We report the sequencing of a gene isolated from B. fragilis 1126 which also confers tetracycline resistance. Because of its high degree of identity (97%) with the tetA(Q)1 gene, we defined it as tetA(Q)2. MIC studies revealed that tetA(Q)2 provides a low level of resistance to tetracycline when cloned into Escherichia coli. The extensive homology between tetA(Q)1 and tetA(Q)2 supports the idea of a recent horizontal transfer of tet(Q) genes among Bacteroides spp.
Lépine
G
G
Periodontal Disease Research Center, College of Dentistry, University of Florida, Gainesville 32610.
Lacroix
J M
JM
Walker
C B
CB
Progulske-Fox
A
A
D000595:Amino Acid Sequence; D001441:Bacteroides fragilis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis; D012689:Sequence Homology, Nucleic Acid; D013753:Tetracycline Resistance / Q000235:genetics; D014169:Transformation, Bacterial
D004274:DNA, Recombinant