Monoclonal antibody anti-type I and anti-zebrin II labelling in siluriform fishes: the role of shared lineage versus shared function in polypeptide co-distributions.
Two monoclonal antibodies (mabs), the newly generated mab anti-type I and the previously described mab anti-zebrin II, were reacted with brainstem sections of two ostariophysan siluriforms, the gymnotoid Rhamphichthys rostratus and the siluroid Ictalurus punctatus. Mab anti-type I recognizes a 47 kDa polypeptide present in the dendrites and soma of projection neurons. Mab anti-zebrin II recognizes a 36 kDa polypeptide present throughout the neuronal cytoplasm, including the axon. Strongly type I immunopositive cells include: all cerebellar Purkinje cells; pyramidal cells of the nucleus medialis, electrosensory lateral line lobe and tectum; pacemaker relay cells; Mauthner neurons; lateral line ganglion cells; cells of the inferior olive; and large neurons of the reticular formation and lateral reticular nucleus. Weakly reactive type I cells include: neurons in the torus semicircularis, medial and efferent octavolateralis nuclei, the magnocellular and lateral tegmental nuclei; and the motor neurons of the Vth, VIIth and Xth cranial nerves. Most type I positive cells are brainstem projection neurons. Zebrin II expression is restricted to subsets of two cell types which also express the type I antigen - Purkinje cells and acousticolateralis pyramidal cells. Both of these neuronal types develop from the region of the rhombic lip. While the mutual expression of the type I antigen can be explained by the shared function of projection neurons, the common expression of the zebrin II antigen is most likely due to a shared embryological and/or phylogenetic lineage.
Hoggatt
A M
AM
Muncie Center for Medical Education, Indiana University School of Medicine, Ball State University 47306.
Lannoo
M J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal; D001933:Brain Stem / Q000166:cytology / Q000378:metabolism*; D005399:Fishes / Q000378:metabolism*; D007059:Ictaluridae; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D010802:Phylogeny; D011689:Purkinje Cells / Q000378:metabolism; D017966:Pyramidal Cells / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins; D010455:Peptides; C092565:zebrin I; C071871:zebrin II