Proliferation of human leukemic pre-B cells induced by human bone marrow stromal cells and murine fibroblasts.
Aim of the study was the establishment of a standardized in vitro culture system for studies on proliferation and differentiation of human leukemic pre-B cells. Coincubation with human stromal cells led to a significantly higher proliferation of the cytokine-sensitive leukemic pre-B cell line BLIN-1. Clones from the murine fibroblast cell line L929 provided identical results. Coincubation with the murine cells also resulted in a significantly higher numbers of viable cells in 5 of 8 patient samples with newly diagnosed B lineage ALL. The results show that in vitro bone marrow stromal cells can be substituted by murine fibroblasts and form the basis for a simpler and more reproducible assay system.
Buske
C
C
Dept. Internal Medicine, Georg-August-Universität, Göttingen, Germany.
Hiddemann
W
W
Wörmann
B
B
D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D003238:Connective Tissue / Q000502:physiology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004338:Drug Combinations; D006801:Humans; D015851:Interleukin-7 / Q000494:pharmacology; D007739:L Cells / Q000502:physiology*; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*; D011509:Proteoglycans / Q000494:pharmacology; D015203:Reproducibility of Results; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D004338:Drug Combinations; D015851:Interleukin-7; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen