A sensitive, specific immunobioassay for quantitation of human interleukin 6.
Picogram quantities of human interleukin 6 (h-IL-6) were detected by a two-step method. A microtiter plate coated with anti-h IL-6 monoclonal antibody was used to capture the IL-6 present in biological samples. An IL-6- dependent B cell line (7TD1) that proliferates in response to IL-6 was added to the captured IL-6. The lower limit of detection for this immunobioassay with serum or cell culture supernatants was 5 pg/ml. The specificity of the assay was achieved by the antibody used in the first step. The sensitivity was provided by the IL-6-dependent cell line. The method also allows for the removal of inhibitors, metabolites, antagonists or activating agents used to induce IL-6. This immunobioassay has the advantage over other current methods in that it measures immunoactive as well as biologically active IL-6.
Krakauer
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Applied Research Division, U.S. Army Medical Research, Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011.
D000911:Antibodies, Monoclonal / Q000276:immunology; D016477:Artifacts; D001402:B-Lymphocytes / Q000187:drug effects*; D001681:Biological Assay / Q000379:methods*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000097:blood; D006801:Humans; D006825:Hybridomas / Q000187:drug effects; D015850:Interleukin-6 / Q000032:analysis* / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008213:Lymphocyte Activation; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D015850:Interleukin-6; D011994:Recombinant Proteins