Cloning and expression of the chicken CD18 cDNA.
The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. We describe here the isolation and characterization of the chicken beta 2 (CD18) subunit, common to the leukocyte integrin family. The deduced 748-amino-acid sequence reveals a transmembrane protein with 65% and 64% identity with its human and murine homologues, respectively. The chicken beta 2 can associate on the cell surface with the human alpha subunit of LFA-1 and yields a hybrid molecule capable of binding to purified ICAM-1 and ICAM-3.
Bilsland
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Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115.
Springer
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D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D000943:Antigens, Differentiation; D001483:Base Sequence; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data
D015703:Antigens, CD; D000943:Antigens, Differentiation; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; C483616:ICAM3 protein, human; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1