Nucleotide sequence of the phosphotransacetylase gene of Escherichia coli strain K12.
The phosphotransacetylase gene (pta) from Escherichia coli strain K-12 1100 was identified in a cloned fragment of chromosomal DNA (Yamamoto-Otake, H., Matsuyama, A. and Nakano, A. (1990) Appl. Microbiol. Biotechnol. 33, 680-682). Overexpression in E. coli confirmed the presence of the pta gene within the cloned fragment. DNA sequence analysis of the cloned pta gene indicates that the predicted phosphotransacetylase polypeptide chain is 713 amino acids in length. The carboxyterminal region of the E. coli phosphotransacetylase shows 42.6% sequence identity with the corresponding enzyme from Methanosarcina thermophila (142 out of 333 residues in corresponding positions are identical). Several short regions of high sequence identity may be structurally or functionally important for enzymic activity.
Matsuyama
A
A
Kikkoman Corporation Research and Development Division, Chiba, Japan.
Yamamoto-Otake
H
H
Hewitt
J
J
MacGillivray
R T
RT
Nakano
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D017020:Methanosarcina / Q000235:genetics; D008969:Molecular Sequence Data; D010709:Phosphate Acetyltransferase / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D010709:Phosphate Acetyltransferase