[A study of DNA damage induced by asbestos fibers in vitro].
The results of our in vitro study showed that Rhodesia chrysotile, X. K. chrysotile, UICC chrysotile, crocidolite and amosite could cause damage to calf thymus DNA by inducing the production of 8-hydroxy-2'-deoxyguanosine (8-OH-dG). After adding H2O2 and/or FeSO4, the production of 8-OH-dG, induced by asbestos fibers was significantly increased. The fiber of UICC crocidolite has the most untoward effect, that of UICC amosite comes next, and the three chrysotile fibers are the weakest ones. The effect of asbestos fibers on the production of 8-OH-dG has some relationship with hydroxy free radical and the type of asbestos. It is suggested that asbestos fibers could induce DNA point mutation through A.T and G.C transversion by the production of 8-OH-dG, which is related with the carcinogenesis of asbestos.
Zhang
J
J
Hospital of Chuxiong, Yunnan.
Sun
C
C
Yao
Q
Q
Lian
X
X
Ke
F
F
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D017639:Asbestos, Amosite / Q000633:toxicity; D017638:Asbestos, Crocidolite / Q000633:toxicity; D017632:Asbestos, Serpentine / Q000633:toxicity; D002417:Cattle; D003043:Cocarcinogenesis; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000096:biosynthesis; D004357:Drug Synergism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D058085:Iron Compounds / Q000494:pharmacology
D017632:Asbestos, Serpentine; D058085:Iron Compounds; D017638:Asbestos, Crocidolite; D017639:Asbestos, Amosite; D001194:Asbestos; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D006861:Hydrogen Peroxide; D003849:Deoxyguanosine