Identity of non-malignant human urothelial cell lines classified as transformation grade I (TGrI) and II (TGrII).
Restriction fragment length polymorphism (RFLP) and marker chromosome or isozyme analysis were used to study the identity of 7 human urothelial cell lines selected according to their HLA-A,B phenotype. The results confirmed that the cell lines Hu961b, Hu1125, Hu1694, Hu1752, Hu609 and HCV-29 were genuine cell lines of different origin, while Hu1734 and HCV-29 were of the same origin. Hu609 was recorded to be derived from a female. However, the presence of a Y chromosome indicated either false labelling or cross-contamination. Analysis of a series of frozen stocks of Hu609 cultures showed that "malignant transformation" of these cultures was due to cross-contamination.
Christensen
B
B
Fibiger Institute, Danish Cancer Society, Copenhagen.
Hansen
C
C
Kieler
J
J
Schmidt
J
J
D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002874:Chromosome Mapping; D004848:Epithelium / Q000276:immunology / Q000502:physiology; D005819:Genetic Markers; D005914:Globins / Q000235:genetics; D015234:HLA-A Antigens / Q000032:analysis; D015235:HLA-B Antigens / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D017403:In Situ Hybridization; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D001743:Urinary Bladder / Q000276:immunology / Q000502:physiology*; D014998:Y Chromosome
D005819:Genetic Markers; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D005914:Globins