[Analysis of mdr-1 mRNA and P 170 glycoprotein in cytotoxic resistant sublines].
Multidrug resistant sublines KBV200 and HCTV2000 were established by continuous selection from human KB and HCT cell lines cultured in medium in the presence of vincristine. The mdr-1 mRNA in total RNA from the two sublines was detected by dot blotting with HRP-labelled mdr-1 cDNA probe and enhanced chemiluminescent autography and the P170 glycoprotein in their cells was detected by immunohistochemistry. The mdr-1 gene expression level of KBV200 and HCTV2000 was higher than their wild counterparts. It was shown that the drug resistant properties of the two sublines were related to mdr-1 gene expression. The non-radioactive technique for analysis of specific mRNA and protein could be generalized for clinical research of gene expression.
Wang
S W
SW
Institute of Hematology, Beijing Medical University, Beijing.
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism; D015342:DNA Probes; D004351:Drug Resistance / Q000235:genetics*; D006801:Humans; D007938:Leukemia / Q000235:genetics; D008163:Luminescent Measurements; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism; D009857:Oncogenes; D012333:RNA, Messenger / Q000032:analysis*; D014407:Tumor Cells, Cultured; D014750:Vincristine / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins; D015342:DNA Probes; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D014750:Vincristine