title: Open-chain peptides obtained by acidic hydrolytic cleavage of cyclosporin A.
abstract: Hydrolysis of cyclosporin A (CsA) was studied in order to clarify the still undefined point of attack of the acidic degradation. Among ether extractable and water-soluble products formed from CsA in HCl, two open-chain peptides were isolated by high-performance liquid chromatography which were identified as the deca- and nonapeptides deriving from CsA through the hydrolytic cleavage of amino acid residue 11 and both residues 11 and 10, respectively. Identification was carried out by fast atom bombardment tandem mass spectrometry.
authors.affiliation: Scientific Institute H. S. Raffaele, Milan, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Magni
authors.forename: C
authors.initials: C
authors.lastname: Arcelloni
authors.forename: R
authors.initials: R
authors.lastname: Paroni
authors.forename: I
authors.initials: I
authors.lastname: Fermo
authors.forename: P A
authors.initials: PA
authors.lastname: Bonini
authors.forename: M
authors.initials: M
authors.lastname: Del Puppo
authors.forename: A
authors.initials: A
authors.lastname: Manzocchi
authors.forename: M
authors.initials: M
authors.lastname: Galli Kienle
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009842:Oligopeptides; D016572:Cyclosporine