title: Synergistic interactions of CD4+ and CD8+ T cell subsets with human vascular endothelial cells in primary proliferative allogeneic responses.
abstract: The ability of subcultured human vascular endothelial cells (EC) to provide immune accessory functions for proliferative responses of highly purified allogeneic CD4+ and CD8+ T cells has been examined. CD4+ T cells proliferated in response to IFN-gamma-pretreated EC which expressed class II molecules, but not to untreated EC. CD8+ T cells proliferated to MHC class I molecules expressed on both untreated and IFN-gamma-treated EC. Combined populations of CD4+ and CD8+ T cells showed synergistic, rather than additive, responses to both untreated and IFN-gamma-treated EC. Furthermore, CD8+ T cells were able to induce MHC class II expression on endothelial cells and this induction could be inhibited by an anti-IFN-gamma mAb. The synergistic response obtained by co-culturing CD4+ and CD8+ T cells with vascular EC was completely inhibited by the same anti-IFN-gamma mAb. These studies suggest that CD4+ and CD8+ T cells recognise and proliferate to allogeneic MHC molecules expressed by EC. CD4+ and CD8+ responses are synergistic under the conditions tested and this synergism appears to be due to induction of MHC class II antigens on EC by IFN-gamma secreted from CD8+ T cells.
authors.affiliation: Vascular Biology Team, Clinical Research Centre, Harrow, UK.
authors.forename: C J
authors.initials: CJ
authors.lastname: Brooks
authors.forename: A
authors.initials: A
authors.lastname: Stackpoole
authors.forename: C O
authors.initials: CO
authors.lastname: Savage
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002450:Cell Communication; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000276:immunology*; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014471:Umbilical Veins
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma