Regeneration of the dopamine-cell mosaic in the retina of the goldfish.
A fundamental anatomical feature of retinal neurons is that they form planar mosaics. Each mosaic can be described by its density, pattern, and regularity (non-randomness). As part of ongoing studies to quantitatively describe the anatomy of regenerated retina in the goldfish, we determined the planimetric density and regularity of the mosaic of dopaminergic interplexiform cells in patches of regenerated retina and compared this to the mosaic generated de novo. In addition, we selectively ablated dopaminergic neurons with the neurotoxin 6-hydroxydopamine (6-OHDA) before inducing local regeneration and determined whether or not the absence of the extant dopaminergic neurons modulated the planimetric density or number of regenerated ones. The results showed that dopaminergic neurons are regenerated at higher planimetric densities and in less orderly arrays than normal. Furthermore, there was no statistical difference in the density or number of regenerated cells in normal retinas and retinas treated with 6-OHDA.
Hitchcock
P F
PF
W. K. Kellogg Eye Center, Department of Ophthalmology, School of Medicine, University of Michigan, Ann Arbor 48105.
Vanderyt
J T
JT
D000818:Animals; D001973:Bromodeoxyuridine; D002452:Cell Count; D002454:Cell Differentiation; D002455:Cell Division; D004261:DNA Replication; D004298:Dopamine / Q000378:metabolism*; D006054:Goldfish; D007395:Interneurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008856:Microscopy, Fluorescence; D016627:Oxidopamine / Q000494:pharmacology; D012038:Regeneration / Q000502:physiology*; D012160:Retina / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D001973:Bromodeoxyuridine; D004298:Dopamine