A study of criteria permitting the use of plastinated specimens for light and electron microscopy.
Plastination permits the preservation of anatomical specimens in a physical state approaching that of the living condition. We studied the possibility of using silicone plastinated fragments of spleen and pancreas for optical and electron microscopy, and found that with an adequate fixation protocol, plastinated specimens can be used for both light microscopy and ultrastructural studies. Deplastination with sodium methoxide permitted production of clean sections. Artifacts produced by plastination/deplastination could be nearly eliminated by glutaraldehyde/formaldehyde fixation. The (Biodur) silicone S10 polymer is transparent and stable in an electron beam, and plastinated tissues can be contrasted or colored similar to tissues embedded in Epon 812. In addition to being very life-like, plastinated tissues are stable and easy to handle. They can also be used for electron and light microscopic studies. This technique may also allow retrospective epidemiological studies of archived pathology specimens.
Grondin
G
G
Department of Biology, Faculty of Sciences, University of Sherbrooke, Québec, Canada.
Grondin
G G
GG
Talbot
B G
BG
D000818:Animals; D004853:Epoxy Resins; D008027:Light; D008297:Male; D008853:Microscopy / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D010179:Pancreas / Q000648:ultrastructure*; D016612:Paraffin Embedding / Q000379:methods; D016613:Plastic Embedding / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012828:Silicones; D013154:Spleen / Q000648:ultrastructure*; D016707:Tissue Fixation / Q000379:methods
D004853:Epoxy Resins; D012828:Silicones; C004875:EPON