Mediation of renal cyst formation by hepatocyte growth factor.
Hepatocyte growth factor (HGF) is a potent mitogen for renal tubular cells, and HGF and its receptor (c-met proto-oncogene product, Met) can induce lumen formation in epithelial cells. We measured the concentration of HGF in cyst fluids from patients with renal cystic diseases. The concentration of HGF was high in proximal cyst fluid (mean 2.45 vs 0.42 ng/mL in distal cysts). Cyclic AMP concentration was higher in distal than in proximal cysts (663 vs 6.0 pmol/L). mRNA of HGF and Met were co-expressed in cyst walls from cases with polycystic kidney disease. These findings suggest that HGF is a growth factor that may mediate human renal cyst genesis.
Horie
S
S
Department of Urology, Faculty of Medicine, University of Tokyo, Japan.
Higashihara
E
E
Nutahara
K
K
Mikami
Y
Y
Okubo
A
A
Kano
M
M
Kawabe
K
K
D001483:Base Sequence; D015139:Blotting, Southern; D003404:Creatinine / Q000032:analysis / Q000097:blood; D000242:Cyclic AMP / Q000302:isolation & purification; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D017228:Hepatocyte Growth Factor / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D016891:Polycystic Kidney, Autosomal Dominant / Q000737:chemistry* / Q000473:pathology; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics; D019859:Proto-Oncogene Proteins c-met; D012333:RNA, Messenger / Q000032:analysis; D011863:Radioimmunoassay; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis* / Q000235:genetics; D012964:Sodium / Q000032:analysis
D017931:DNA Primers; D018076:DNA, Complementary; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D017228:Hepatocyte Growth Factor; D012964:Sodium; D003404:Creatinine; D000242:Cyclic AMP; D019859:Proto-Oncogene Proteins c-met; D020794:Receptor Protein-Tyrosine Kinases