H-ras-1 restriction fragment length polymorphism in normal individuals and oral cancer patients in India.
Restriction fragment length polymorphism (RFLP) of the human H-ras-1 gene has been indicated as a marker for detection of individuals at high risk of cancer. We have investigated the association of RFLP at the H-ras-1 locus and susceptibility to oral cancer by Southern hybridization analysis in 77 primary oral tumors and 99 healthy donors. The frequency distribution of the BamHI fragments of H-ras-1 revealed homozygous or heterozygous alleles in the two subpopulations. The heterozygous genotype occurred more frequently in the normal subjects (53%) as compared to the cancer patients (36%). Four common alleles-C1 to C4, were noted predominantly in both groups, with rare alleles detected at a lower frequency. The common allele with 7.6 kb BamHI fragment was significantly higher in normals (10%) than in the tumor population (4%) (P < 0.05). However, a similar distribution of rare alleles in both groups indicated that the presence of rare alleles is not indicative of predisposition to oral cancer.
Bhoite
L T
LT
Cell & Developmental Pathology Division, Cancer Research Institute, Parel, Bombay, India.
Saranath
D
D
Nair
R
R
Deo
M G
MG
Sanghavi
V
V
Mehta
A
A
D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D004912:Erythrocytes / Q000502:physiology; D005260:Female; D005787:Gene Frequency; D011905:Genes, ras / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D007194:India; D008297:Male; D009061:Mouth Mucosa / Q000502:physiology*; D009062:Mouth Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D004273:DNA, Neoplasm