title: Isolation and sequence determination of cDNA encoding human T-protein of the glycine cleavage system.
abstract: We present the full-length cDNA sequence of human T-protein of the glycine cleavage system. Overlapping cDNA clones were isolated by screening human liver cDNA libraries with bovine T-protein cDNA. The 1209 base pair open reading frame encodes 403 amino acid precursor protein, and the deduced amino acid sequence of the mature peptide shows 90 and 68% homology to that of bovine and chicken counterpart, respectively. RNA blot analysis of human liver transcripts and Southern blot analysis of human genomic DNA confirm that human T-protein is encoded by a single gene.
authors.affiliation: Department of Pediatrics, Akita University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hayasaka
authors.forename: K
authors.initials: K
authors.lastname: Nanao
authors.forename: G
authors.initials: G
authors.lastname: Takada
authors.forename: K
authors.initials: K
authors.lastname: Okamura-Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Motokawa
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D050960:Aminomethyltransferase; D000818:Animals; D001483:Base Sequence; D004247:DNA; D006801:Humans; D019877:Hydroxymethyl and Formyl Transferases; D008099:Liver / Q000196:embryology / Q000201:enzymology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D014166:Transferases / Q000235:genetics*
chemical_list: D004247:DNA; D014166:Transferases; D019877:Hydroxymethyl and Formyl Transferases; D050960:Aminomethyltransferase