Relationship of a mouse Sertoli cell line (MSC-1) to normal Sertoli cells.
Recently, a mouse Sertoli cell line (MSC-1) was established from transgenic mice carrying a fusion gene composed of human Müllerian inhibitory substance transcriptional regulatory sequences linked to the SV40 T-antigen gene. This cell line contained a morphologically heterogeneous population of cells that expressed characteristic Sertoli cell mRNAs such as transferrin, the beta-subunit for inhibin, and sulfated glycoprotein-2 (SGP-2). In this study, we compared various characteristics of MSC-1 cells to primary cultures of Sertoli cells from immature rats or adult mice. Our observations indicated that: 1) These cells were ultrastructurally similar to mouse Sertoli cells in culture; 2) MSC-1 cells expressed mRNA for androgen-binding protein (ABP) and SGP-1, but not the receptor for FSH; 3) The expression of SGP-2 mRNA and secretion of SGP-2 increased approximately 2-fold when cells were cultured at 41 degrees C, the nonpermissive temperature for the SV40 virus, while SGP-1 and transferrin mRNA levels and secretion were unaffected; 4) Proliferation of these cells was serum-dose dependent and temperature dependent and could be inhibited by incubating MSC-1 cells at 41 degrees C; 5) Proliferation was also significantly reduced after cells were incubated in the presence of dibutyryl cAMP (dbcAMP) for 6 days; 6) Fifteen subcultures produced from single MSC-1 cells displayed similar levels of mRNA expression for transferrin or SGP-1. Together these data indicate that the MSC-1 cell line is composed of a single cell type displaying numerous characteristics of Sertoli cells. This cell line may provide a unique source of tissue for studying various aspects of Sertoli cell behavior.
McGuinness
M P
MP
Department of Biochemistry and Biophysics, Washington State University, Pullman 99164-4460.
Linder
C C
CC
Morales
C R
CR
Heckert
L L
LL
Pikus
J
J
Griswold
M D
MD
D000727:Androgen-Binding Protein / Q000235:genetics; D000818:Animals; D054304:Anti-Mullerian Hormone; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002455:Cell Division; D002460:Cell Line; D051152:Clusterin; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006131:Growth Inhibitors / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018832:Molecular Chaperones; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011962:Receptors, FSH / Q000235:genetics; D011993:Recombinant Fusion Proteins; D012045:Regulatory Sequences, Nucleic Acid; D049231:Saposins; D012708:Sertoli Cells / Q000378:metabolism* / Q000648:ultrastructure; D013735:Testicular Hormones / Q000235:genetics; D014168:Transferrin / Q000235:genetics / Q000378:metabolism
D000727:Androgen-Binding Protein; D000952:Antigens, Polyomavirus Transforming; C495243:Clu protein, mouse; D051152:Clusterin; D006023:Glycoproteins; D006131:Growth Inhibitors; D018832:Molecular Chaperones; C483345:PSAP protein, human; C483344:Psap protein, mouse; C483347:Psap protein, rat; D012333:RNA, Messenger; D011962:Receptors, FSH; D011993:Recombinant Fusion Proteins; D049231:Saposins; D013735:Testicular Hormones; D014168:Transferrin; D054304:Anti-Mullerian Hormone