title: Sequence specificity of tetrahydrobenzopsoralen photobinding to DNA.
abstract: The sequence specificity of photobinding to DNA of two tetrahydrobenzopsoralen derivatives has been investigated by testing the photoreactivity toward a number of self-complementary oligonucleotides. The thermodynamic constant for noncovalent binding to each DNA sequence was evaluated. The extent of photoreactivity was greatly dependent upon base composition. The two tetracyclic compounds show similar behavior in comparison to other bifunctional derivatives. Their overall rate constants were greatly enhanced in comparison to classical psoralens. However, their high efficiency of covalent binding is counterbalanced by low affinity for noncovalent interaction with DNA.
authors.affiliation: Department of Pharmaceutical Sciences, Padua University, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Mobilio
authors.forename: L
authors.initials: L
authors.lastname: Tondelli
authors.forename: M
authors.initials: M
authors.lastname: Capobianco
authors.forename: O
authors.initials: O
authors.lastname: Gia
mesh_terms: D000225:Adenine; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D011564:Furocoumarins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010777:Photochemistry; D013941:Thymine
chemical_list: D011564:Furocoumarins; C092624:4-hydroxymethyltetrahydrobenzopsoralen; C092623:4-methyltetrahydrobenzopsoralen; D004247:DNA; D000225:Adenine; D013941:Thymine