Influence of physiological concentration of progesterone on CD(4+)- and CD(8+)-activated lymphocytes with interleukin 2 and autologous plasma: difference between healthy donors and patients bearing malignant tumors.
The influence of progesterone at the physiological concentration on normal donor and malignant patient peripheral blood lymphocytes incubated with interleukin 2 (IL-2) was investigated. Analysis of the phenotypes of IL-2-activated lymphocytes revealed that there was a difference in the positive rates of CD4+ and CD8+ cells between normal donors and patients with malignancies. Activated lymphocytes of normal adults older than 35 years of age consisted of 60-100% of CD4+ cells and 0-30% of CD8+ cells. Donors younger than 35 years of age showed a positive rate of CD4+ cells, ranging from 0-100%, while CD8+ cells were between 0-60%. However, in patients with malignancies both CD4+ and CD(8+)-activated lymphocytes diffused from 0-100%. Activated lymphocytes of most patients were CD8+ cell dominant, which reversed the CD4+/CD8+ ratio. Almost all patients at clinical stage IV showed a deviation of the positive rates of CD4+ and CD8+ cells from the normal range. It is an open question whether the prognosis of the patients with normal positive rates of CD(4+)- and CD(8+)-activated lymphocytes is favorable or not.
Kimoto
Y
Y
Department of Oncologic Surgery, Osaka University, Japan.
Tong
F Z
FZ
Taguchi
T
T
D000328:Adult; D000368:Aged; D016516:CD4-CD8 Ratio / Q000187:drug effects*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D016827:CD8 Antigens; D002455:Cell Division / Q000187:drug effects*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009369:Neoplasms / Q000276:immunology*; D011374:Progesterone / Q000494:pharmacology* / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects*
D016827:CD8 Antigens; D007376:Interleukin-2; D011374:Progesterone