Sequence and characterization of the Escherichia coli genome between the ndk and gcpE genes.
The region of the chromosome immediately upstream of the Escherichia coli gene gcpE has been cloned and sequenced. This region contains two functional open reading frames, orf 384 and orf 337, encoding proteins of 43,082 and 36,189 Da, respectively. Sequencing analysis (this paper) and the isolation of a DNA fragment containing a functional promoter (Talukder, A.A., Yanai, S., and Yamada, M. (1994) Biosci. Biotech. Biochem. 58, 117-120) indicate that orf 337 is in an operon with gcpE. The gene orf 384 is immediately downstream of the gene ndk, which encodes nucleoside diphosphate kinase.
Baker
J
J
Department of Microbiology, Southern Illinois University, Carbondale 62901-6508.
Parker
J
J
D001426:Bacterial Proteins / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics; D016366:Open Reading Frames; D009876:Operon; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D009701:Nucleoside-Diphosphate Kinase