title: Developmental competence of oocytes from the ovaries of repeat-breeding cows after in vitro fertilization.
abstract: The present study examined the developmental competence of oocytes from the ovaries of repeat-breeding cows after in vitro fertilization (IVF). The number of oocytes recovered from eight repeat-breeders averaged 45. Following IVF, the average number of blastocysts obtained was 5 (range from 1 to 7) per cow. Twenty-two frozen-thawed blastocysts were transferred to recipient animals and produced nine pregnancies (41%). Eight normal calves were subsequently born.
authors.affiliation: Department of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Hishinuma
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Kanagawa
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D002417:Cattle / Q000502:physiology*; D002455:Cell Division; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D010053:Ovary / Q000166:cytology / Q000502:physiology*; D010298:Parity