Mixed antiglobulin reaction and tray agglutination test for detection of sperm antibodies.
OBJECTIVE
To compare levels of sperm-agglutinating antibodies in male and female sera, cervical ovulatory mucus, and seminal plasma by two different tests.


METHODS
408 women and 404 men with long-lasting unexplained infertility and recurrent IVF failure were immunologically examined. Tray agglutination and mixed antiglobulin reaction tests were used for the comparison of sperm-agglutinating antibodies in sera, in ovulatory cervical mucus, and in seminal plasma.


RESULTS
High secretory IgA positivity predominated in seminal plasma in 16 (3.9%) of infertile men and in cervical ovulatory mucus in 114 (27.9%) of infertile women. Positivity in two sperm antibodies together, in IgG and IgA, and IgM and IgE was also observed. Immunological anti-sperm antibodies in sera indicate high levels of sperm antibodies in IgG and IgM.


CONCLUSIONS
Sperm immunoglobulin specification is important for therapeutic influence upon immunocompetent cells.
Ulcová-Gallová
Z
Z
Department of Obstetrics and Gynaecology, Charles University of Pilsen, Plzen, Czechoslovakia.
D000372:Agglutination Tests; D000906:Antibodies / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007247:Infertility, Female / Q000276:immunology*; D007248:Infertility, Male / Q000276:immunology*; D008297:Male; D013073:Sperm Agglutination / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E