ABFI contributes to the chromatin organization of Saccharomyces cerevisiae ARS1 B-domain.
The involvement of the ABFI transcription factor in organizing the chromatin structure of the Saccharomyces cerevisiae ARS1 region has been previously postulated. We studied the ARS1 chromatin structure both on the chromosome and on plasmids carrying wild type or mutated ABFI binding sites, using a recently developed no-background technique for nucleosome mapping, coupled with high resolution micrococcal nuclease in vivo footprinting. We show that ABFI protein acts as a boundary element of chromatin structure, by limiting the invasion by nucleosomes toward the essential A-domain.
Venditti
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P
Centro di Studio per gli Acidi Nucleici, CNR, Rome, Italy.
Costanzo
G
G
Negri
R
R
Camilloni
G
G
D001483:Base Sequence; D002843:Chromatin / Q000235:genetics* / Q000648:ultrastructure; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D008836:Micrococcal Nuclease / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D018741:Replication Origin / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism*
C061886:ABF1 protein, S cerevisiae; D002843:Chromatin; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D008836:Micrococcal Nuclease