Prenatal determination of fetal rhesus D status by DNA amplification of peripheral blood of rhesus-negative mothers.
We have developed a sensitive PCR-based assay for the RhD gene and used it to detect circulating fetal cells from RhD-positive fetuses from peripheral blood of RhD-negative mothers. With further improvement in diagnostic accuracy, this assay may have implications in the management of RhD-sensitized pregnancies in women whose partners are heterozygous for the RhD gene. Further studies are required to determine the relationship between maternal anti-D levels and circulating fetal cell numbers.
Lo
Y M
YM
Nuffield Department of Pathology & Bacteriology, John Radcliffe Hospital, Oxford, United Kingdom.
Bowell
P J
PJ
Selinger
M
M
Mackenzie
I Z
IZ
Chamberlain
P
P
Gillmer
M D
MD
Elliott
P
P
Pratt
G
G
Littlewood
T J
TJ
Fleming
K A
KA
D000653:Amniotic Fluid / Q000166:cytology; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D005333:Fetus / Q000166:cytology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy / Q000097:blood*; D011296:Prenatal Diagnosis / Q000379:methods*; D012203:Rh Isoimmunization / Q000175:diagnosis*; D012204:Rh-Hr Blood-Group System / Q000235:genetics
D017931:DNA Primers; D012204:Rh-Hr Blood-Group System