[Detection of monoclonality in non-Hodgkin's B-cell lymphomas using PCR].
OBJECTIVE
To evaluate the reliability of a PCR technique for the detection of monoclonal B-cell non-Hodgkin's lymphoma (NHL) in a group of patients previously showing monoclonal gene rearrangement with Southern Blot techniques.


METHODS
A group of 22 cases of NHL were studied with immunocytochemical techniques by means of a complete monoclonal antibody panel after previous demonstration of monoclonal rearrangement with the JH probe. Specific Fr2 and Fr3 priming of the variable regions of the IgH genes was used. One positive and two negative controls were used on each PCR test.


RESULTS
Monoclonal lymphoma was detected in 18 cases (82%), seven of them with Fr2 and Fr3, whereas five cases had Fr2 and the remainders had only Fr3.


CONCLUSIONS
PCR seems a good alternative for detecting monoclonal lymphoproliferative syndromes instead of Southern Blot due to both its specificity and high sensitivity. The hypotheses to explain false negative results are discussed.
Robledo
M
M
Fundación Jiménez Díaz, Departamento de Genética, Madrid.
Martínez
B
B
Arranz
E
E
Marcos
B
B
Rivas
C
C
Benítez
J
J
D000911:Antibodies, Monoclonal / Q000235:genetics*; D000912:Antibodies, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D005819:Genetic Markers; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D004273:DNA, Neoplasm; D005819:Genetic Markers; D007143:Immunoglobulin Heavy Chains; D007144:Immunoglobulin J-Chains; D007135:Immunoglobulin Variable Region; D009363:Neoplasm Proteins
Detección de monoclonalidad en linfomas no Hodgkin B mediante PCR.