Modification of the radiosensitivity of human testicular cancer cells by simian virus 40 sequences.
CRL7800 cells are of human testicular cancer origin and are sensitive to killing by ionizing radiation. After transfection with a plasmid expressing the T-antigen (pSC), cells show enhanced growth and an increased resistance to ionizing radiation. Cell cycle analysis reveals perturbation of a cell cycle checkpoint which, after irradiation, results in an increase in G2-phase arrest in CRL7800VA cells. These experiments demonstrate the modulation of radiation sensitivity and cell cycle arrest of human tumor cells by the introduction of viral genes.
Jung
M
M
Department of Radiation Medicine, Georgetown University School of Medicine, Washington, D.C. 20007.
Dritschilo
A
A
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002470:Cell Survival / Q000528:radiation effects; D016195:G2 Phase; D006801:Humans; D008297:Male; D010957:Plasmids; D011836:Radiation Tolerance; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D013736:Testicular Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D014162:Transfection; D016662:Virus Integration
D000952:Antigens, Polyomavirus Transforming