Metallothionein mRNA in the testis and prostate of the rat detected by digoxigenin-labeled riboprobe.
Metallothionein (MT), a cysteine-rich heavy metal-binding protein, has been considered to play a role in the homeostatic control and detoxification of heavy metals, such as zinc, copper, and cadmium. In the present study, we have utilized a digoxigenin-labeled riboprobe to localize MT mRNA only by bright-field optics in the testis and prostate of the rat. In the rat testis, MT mRNA was found predominantly in primary spermatocytes and also in secondary spermatocytes and spermatids, but not in the spermatogonia, Sertoli cells, and Leydig cells. On the other hand, MT protein was present in these spermatogenic cells as well as in spermatozoa and Sertoli cells. In the prostate, MT mRNA was found predominantly in the epithelium of the dorsolateral lobes, but not in the ventral lobe, which is in agreement with the observed localization of MT protein. The utilization of both in situ hybridization and immunohistochemical staining on the same tissue specimens show MT gene expression in specific cell types in the male genital organs.
Tohyama
C
C
Environmental Health Sciences Division, National Institute for Environmental Studies, Ibaraki, Japan.
Nishimura
N
N
Suzuki
J S
JS
Karasawa
M
M
Nishimura
H
H
D000818:Animals; D001360:Avidin; D001710:Biotin; D018076:DNA, Complementary; D004076:Digoxigenin / Q000276:immunology*; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007202:Indicators and Reagents; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis*; D011467:Prostate / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000378:metabolism*
D018076:DNA, Complementary; D007202:Indicators and Reagents; D015347:RNA Probes; D012333:RNA, Messenger; D001360:Avidin; D001710:Biotin; D008668:Metallothionein; D006735:Horseradish Peroxidase; D004076:Digoxigenin