title: No evidence of mycoplasmas in peripheral blood mononuclear cell fraction of HIV-infected patients.
abstract: In this study, the prevalence of mycoplasmas in peripheral blood mononuclear cells (PBMC) from HIV-infected individuals was investigated using a mycoplasma genus-specific PCR assay. No mycoplasmas were detected in the PBMC samples from any of the 25 HIV-infected individuals (CDC 2, n = 8; CDC 3, n = 2; CDC 4, n = 15) or ten HIV-seronegative controls. As an internal control, HIV specific sequences were detected in the samples from all HIV-seropositives. These negative results do not support a suggested role of mycoplasmas as co-factor in the progression of HIV infection towards AIDS.
authors.affiliation: Dept. of Microbiology, University of Nijmegen, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: van Kuppeveld
authors.forename: W
authors.initials: W
authors.lastname: Melchers
authors.forename: J
authors.initials: J
authors.lastname: Kissing
authors.forename: J
authors.initials: J
authors.lastname: van der Logt
authors.forename: J
authors.initials: J
authors.lastname: Galama
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000097:blood / Q000150:complications / Q000453:epidemiology*; D001483:Base Sequence; D016022:Case-Control Studies; D004269:DNA, Bacterial; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D009175:Mycoplasma Infections / Q000097:blood* / Q000150:complications / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012307:Risk Factors
chemical_list: D004269:DNA, Bacterial