title: Monoclonal antibodies that recognize the type-2 activin receptor, ACTR2.
abstract: Monoclonal antibodies (MAbs) were raised in mice against a bacterial fusion protein composed of the intracellular serine/threonine kinase domain of the type-2 activin receptor, ACTR2, fused to glutathione S-transferase. Three MAbs with high affinity toward the ACTR2 kinase domain were isolated, one of which recognized specifically ACTR2 expressed transiently in vascular endothelial cells. These reagents should be of use in the elucidation of mechanisms of transmembrane signaling by this member of the emerging receptor serine threonine kinase family.
authors.affiliation: Molecular Angiogenesis Group, Imperial Cancer Research Fund, Oxford, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: McCarthy
authors.forename: H
authors.initials: H
authors.lastname: Turley
authors.forename: K C
authors.initials: KC
authors.lastname: Gatter
authors.forename: R
authors.initials: R
authors.lastname: Bicknell
mesh_terms: D029404:Activin Receptors; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D002417:Cattle; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000276:immunology*; D017978:Receptors, Growth Factor / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013917:Thrombin / Q000378:metabolism; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D017978:Receptors, Growth Factor; D011993:Recombinant Fusion Proteins; D017346:Protein Serine-Threonine Kinases; D029404:Activin Receptors; D013917:Thrombin