HMG-X, a Xenopus gene encoding an HMG1 homolog, is abundantly expressed in the developing nervous system.
We used a PCR-based subtraction cloning procedure with Concanavalin A-treated and untreated animal caps from stage 9 Xenopus embryos to search for genes the expression of which is induced during neurogenesis. One of these genes was found to encode a homolog of mammalian HMG 1 and 2, hence named HMG-X. HMG-X mRNA was maternally transmitted, up-regulated in neuroectoderm-derived tissues throughout early development, and eventually down-regulated in all adult tissues examined except ovary. Our data suggest that we have identified a gene for a member of the HMG1/2 family that could have an important role in neurogenesis.
Kinoshita
M
M
Department of Viral Oncology, Cancer Institute, Tokyo, Japan.
Hatada
S
S
Asashima
M
M
Noda
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002490:Central Nervous System / Q000196:embryology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D015536:Down-Regulation; D004475:Ectoderm; D004627:Embryonic Induction; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation; D029867:Xenopus Proteins; D014982:Xenopus laevis
D002352:Carrier Proteins; D018076:DNA, Complementary; C089648:HMG-X protein, Xenopus laevis; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D029867:Xenopus Proteins