title: Cloning and sequencing of the gene for the lactose carrier of Citrobacter freundii.
abstract: The gene coding for the lactose carrier of Citrobacter freundii was cloned into the plasmid pBR322. The gene was sequenced and the amino acid sequence was found to be 70% identical to the lactose carrier of E. coli. All of the charged residues in the membrane spanning region were conserved. The sugar specificity is somewhat different from that of E. coli. The C. freundii carrier has less activity for lactose and more activity for o-nitrophenyl-galactose (ONPG) than the carrier of E. coli.
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115.
authors.forename: J I
authors.initials: JI
authors.lastname: Lee
authors.forename: N
authors.initials: N
authors.lastname: Okazaki
authors.forename: T
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: T H
authors.initials: TH
authors.lastname: Wilson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016971:Citrobacter freundii / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D015698:Genomic Library; D005838:Genotype; D007709:Klebsiella / Q000235:genetics; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D009598:Nitrophenylgalactosides / Q000378:metabolism; D010957:Plasmids; D017386:Sequence Homology, Amino Acid; D027981:Symporters
chemical_list: D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; D009598:Nitrophenylgalactosides; C059200:4-nitrophenylgalactoside; C021499:lactose permease