Identification and characterization of an Onchocerca volvulus cDNA clone encoding a highly immunogenic calponin-like protein.
The identification and characterization of a recombinant cDNA clone, designated OV9M, expressing an antigen present in Onchocerca volvulus infective larvae and adult stages is described. Clone OV9M was identified by screening a lambda gt11 cDNA expression library derived from adult O. volvulus mRNA using pooled rabbit antisera raised against the third (L3) and fourth (L4) stage larvae of the parasite. The cDNA clone encodes an open reading frame of 238 amino acids corresponding to a 27-kDa polypeptide. This polypeptide contains a series of five highly conserved repeats of 25 amino acids that are similar to repeats found in calponin, a protein previously only identified in vertebrate smooth muscle. Extension of the 5' end of the cDNA clone revealed two additional repeats extending the sequence to 378 amino acids, encoding a 41.8-kDa protein. Affinity purified antibodies, which bound specifically to the glutathione S-transferase-OV9M fusion polypeptide, recognize a series of antigens in extracts of O.volvulus microfilariae, L3, L4 and adult stages. The apparent molecular weight of the native OV9M protein in the adult is 45 kDa. Similar proteins are present in extracts of other nematodes including Caenorhabditis elegans, and antibodies from other filarial infections are cross reactive with glutathione S-transferase-OV9M fusion polypeptide. Immunoelectron microscopy revealed that the antigen encoded by this clone is present in the longitudinal muscles of the various larval stages and adult worms. Antibodies to the OV9M protein are present in 40-60% of both patently infected and non-patent individuals residing in onchocerciasis endemic areas.
Irvine
M
M
Lindsley F. Kimball Research Institute, New York Blood Center, NY 10021.
Huima
T
T
Prince
A M
AM
Lustigman
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000378:metabolism; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics*; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008840:Microfilament Proteins; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017181:Onchocerca volvulus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D009855:Onchocerciasis / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D000096985:Calponins
D000947:Antigens, Helminth; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D008840:Microfilament Proteins; D000096985:Calponins