[Design of heterologous mitochondria: import of cattle cytochrome P-450SCC precursors in plant mitochondria].
It has been shown that pre-P-450scc of bovine adrenal cortex mitochondria synthesized in a rabbit reticulocyte lysate cell-free system, is translocated into isolated soybean cotyledon mitochondria, thereby taking the mature form size. This finding is suggestive of the occurrence of a specific receptor and maturase for pre-P-450scc in plant mitochondria. Thus, plant mitochondria can be used as recipients for the mammalian cholesterol hydroxylase system in an attempt to study the mechanism of its formation and preservation.
Luzikov
V N
VN
Novikova
L A
LA
Spiridonova
V A
VA
Isaeva
L V
LV
Whelan
J
J
Hugosson
M
M
Glaser
E
E
D000302:Adrenal Cortex / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology / Q000378:metabolism*
D004792:Enzyme Precursors; D002786:Cholesterol Side-Chain Cleavage Enzyme
Konstruirovanie geterologicheskikh mitokhondriĭ: import predshestvennika tsitokhroma P-450SCC byka v rastitel'nye mitokhondrii.