The accumulation of novobiocin by Escherichia coli and Staphylococcus aureus.
The accumulation of novobiocin by Escherichia coli was shown to be a nonsaturable process. Inhibitors or uncouplers of respiration had no effect on accumulation in cells grown in Isosensitest broth, whereas, bacteria grown in nutrient broth demonstrated an energy-dependent efflux mechanism. In contrast, accumulation of novobiocin by Staphylococcus aureus was found to have a Km of 74 microM and uncouplers and inhibitors of respiration reduced the accumulation suggesting that in S. aureus novobiocin is transported actively.
Barrett-Bee
K
K
Infection Research Department, Zeneca Pharmaceuticals, Cheshire, UK.
Pinder
P
P
D004926:Escherichia coli / Q000378:metabolism*; D009675:Novobiocin / Q000493:pharmacokinetics*; D013211:Staphylococcus aureus / Q000378:metabolism*
D009675:Novobiocin