title: A human opsin-related gene that encodes a retinaldehyde-binding protein.
abstract: The ligand-binding property of a cytoplasmic membrane-bound protein from bovine retinal pigment epithelium (RPE) has been demonstrated. The putative RPE-retinal G protein coupled receptor (RGR) covalently binds both all-trans- and 11-cis-retinal after reduction by sodium borohydride. The 32-kDa receptor binds all-trans-retinal preferentially, rather than the 11-cis isomer. The amino acid sequence of the opsin-related protein in humans is 86% identical to that of bovine RGR, and a lysine residue, analogous to the retinaldehyde attachment site of rhodopsin, is conserved in the seventh transmembrane domain of RGR in both species. The human gene that encodes the novel retinaldehyde receptor spans 14.8 kb and is split into seven exons. The structure of the gene is distinct from that of the visual pigment genes. These findings support the notion that the rgr gene represents the earliest independent branch of the vertebrate opsin gene family. A second form of human RGR in retina is predicted by alternative splicing of its precursor mRNA. This RGR variant results from the alternative use of an internal acceptor splice site in the second intron of the human gene, and it contains an insertion of four amino acids in the connecting loop between the second and thrid transmembrane domains. Since RGR binds all-trans-retinal preferentially, one of its functions may be to catalyze isomerization of the chromophore by a retinochrome-like mechanism.
authors.affiliation: Doheny Eye Institute, University of Southern California, School of Medicine, Los Angeles 90033.
authors.forename: D
authors.initials: D
authors.lastname: Shen
authors.forename: M
authors.initials: M
authors.lastname: Jiang
authors.forename: W
authors.initials: W
authors.lastname: Hao
authors.forename: L
authors.initials: L
authors.lastname: Tao
authors.forename: M
authors.initials: M
authors.lastname: Salazar
authors.forename: H K
authors.initials: HK
authors.lastname: Fong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001894:Borohydrides / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D018076:DNA, Complementary / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010857:Pigment Epithelium of Eye; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D015183:Restriction Mapping; D012172:Retinaldehyde / Q000378:metabolism; D012243:Rhodopsin / Q000737:chemistry; D017299:Rod Opsins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013237:Stereoisomerism
chemical_list: C020932:11-cis-retinal-binding protein; D001894:Borohydrides; D002352:Carrier Proteins; D018076:DNA, Complementary; D005136:Eye Proteins; C089754:G protein-coupled receptor RGR; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D017299:Rod Opsins; C025364:sodium borohydride; D012243:Rhodopsin; D019204:GTP-Binding Proteins; D012172:Retinaldehyde