Antibodies to 68, 52, and 48 kd proteins of Mycobacterium avium in serum samples from patients with Mycobacterium avium infection.
Mycobacterium avium complex (MAC) is responsible for the highest incidence of disseminated bacterial infection in patients with AIDS. Disease caused by this organism was originally thought to be rare in nonimmunocompromised individuals, but it is now being encountered more frequently. Relatively little is known about the components that play a role in the immunopathology of infection caused by MAC. To identify the immunoreactive antigens of this organism that are important targets of the humoral immune response in MAC infection, serum samples from MAC-infected and non-MAC-infected patients were analyzed by Western Blot analysis with sonic extracts of M. avium (MA) as the antigen. The MAC-infected population included patients who were HIV-negative as well as patients with AIDS, and the non-MAC-infected population included non-AIDS, HIV-positive patients and normal, purified protein derivative-negative individuals. The immunodominant antigens recognized by the MAC-infected patients were the 68, 52, and 48 kd proteins of MA. These antigens were also recognized by a few of the non-MAC-infected patients. However, significant differences were observed between the number of MAC-infected and non-MAC-infected patients reacting with these antigens. Sixty-nine percent of the MAC-infected patient serum samples were found to react with the 68 kd antigen, whereas only 24% of the serum samples from patients without MAC infection recognized this antigen (p < 0.001). Of the MAC-infected serum samples, 53.1% were found to react with the 52 kd antigen, whereas only 22% of the non-MAC-infected patient serum samples reacted with this antigen (p = 0.008).(ABSTRACT TRUNCATED AT 250 WORDS)
Rao
S P
SP
Department of Medicine, Pulmonary and Critical Care Division, University of California, San Diego.
Catanzaro
A
A
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000328:Adult; D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D018023:HIV Seronegativity / Q000276:immunology; D006679:HIV Seropositivity / Q000276:immunology; D006801:Humans; D008970:Molecular Weight; D009162:Mycobacterium avium / Q000378:metabolism*; D015270:Mycobacterium avium-intracellulare Infection / Q000097:blood / Q000150:complications / Q000276:immunology*; D012016:Reference Values
D000906:Antibodies; D001426:Bacterial Proteins