title: Myocardial function and metabolism in pig hearts after relief from chronic partial coronary stenosis.
abstract: Metabolic behavior was compared during acute extracorporeal reperfusion after removal of a chronic 4-day partial coronary stenosis in eight pig hearts (RCS group) and during comparable extracorporeal perfusion in seven chronically prepared hearts (Sham group). Coronary stenosis in RCS hearts was induced in the left anterior descending (LAD) artery by partial inflation of a hydraulic occluder to restrict LAD peak phasic velocity by approximately 50%. Regional mechanical shortening was decreased in RCS compared with Sham hearts after 4 days of chronic coronary stenosis [diminished systolic shortening (P < 0.066) with systolic expansion (P < 0.015)] but was comparable to Sham hearts after relief from stenosis. At analogous workloads (left ventricular pressure and heart rate) during reperfusion, metabolic behavior was distinctive between groups. Specifically, compared with Sham hearts, myocardial O2 consumption was selectively increased in RCS hearts (+ 49 delta %, P < 0.026) as was fatty acid oxidation estimated from 14CO2 production from [U-14C]palmitate (+ 60 delta %, P < 0.061) and exogenous glucose utilization measured from the release of 3H2O from [5-3H]glucose (+ 517 delta %, P < 0.025). At the conclusion of the studies, triphenyltetrazolium chloride staining showed no gross evidence of macroinfarction in RCS or Sham hearts, and there was an essentially unremarkable histological survey of anterior myocardium for microscopic necrosis in either group. The level of O2 consumption and preservation of preferred fatty acid utilization indicate that metabolism remains or regains its aerobic pattern of activity in early recovery immediately after removal of chronic partial coronary stenosis.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Cardiology Section, University of Wisconsin Hospital and Clinics, Madison 53792-3248.
authors.forename: A J
authors.initials: AJ
authors.lastname: Liedtke
authors.forename: B
authors.initials: B
authors.lastname: Renstrom
authors.forename: S H
authors.initials: SH
authors.lastname: Nellis
authors.forename: R
authors.initials: R
authors.lastname: Subramanian
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis; D003327:Coronary Disease / Q000503:physiopathology*; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D006321:Heart / Q000502:physiology* / Q000503:physiopathology; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism*; D010101:Oxygen Consumption; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D012016:Reference Values; D013552:Swine; D013599:Systole
chemical_list: D010169:Palmitic Acids; D010725:Phosphocreatine; D002245:Carbon Dioxide; D019308:Palmitic Acid; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D005947:Glucose