title: Limiting dilution analysis of antigen stimulated IL-4 and IFN-gamma production in human mononuclear cell populations.
abstract: Limiting dilution analysis (LDA) of fresh human mononuclear cell populations has previously been used to estimate the frequency of specific B cells, CTL, proliferative T cells, or cells capable of IL-2 production in various clinical situations. Such approaches evaluate the intensity of the response but provide little information concerning the balance between Th1- vs. Th2-like patterns of cytokine gene expression. Here, we describe development of an LDA method to obtain quantitative estimates of the frequency of antigen-specific IFN-gamma or IL-4 producing cells in human peripheral blood. The approach utilizes 3-4 day antigen-mediated restimulation of mononuclear cell populations freshly derived from grass pollen sensitive allergic rhinitis subjects. IFN-gamma and IL-4 production in culture supernatants are determined by ELISA and CT.h4S bioassay. Cytokine production elicited in this assay is CD4 dependent and antigen specific. As such, it provides a useful non-invasive approach for rapid evaluation of low frequency, antigen-induced cytokine production in the circulating repertoire. This method can readily be extended to analysis of other cytokines in other immunologic disorders or in infectious disease states, allowing longitudinal analysis of individuals and facilitating efforts to establish clear correlations between in vivo patterns of cytokine gene expression and disease exacerbation and remission.
authors.affiliation: Department of Immunology, University of Manitoba, Winnipeg, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: W P
authors.initials: WP
authors.lastname: Stefura
authors.forename: F E
authors.initials: FE
authors.lastname: Simons
authors.forename: F T
authors.initials: FT
authors.lastname: Jay
authors.forename: K T
authors.initials: KT
authors.lastname: HayGlass
mesh_terms: D000293:Adolescent; D000328:Adult; D000941:Antigens / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007158:Immunologic Techniques; D007201:Indicator Dilution Techniques; D007371:Interferon-gamma / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D011058:Pollen / Q000276:immunology
chemical_list: D000941:Antigens; D015847:Interleukin-4; D007371:Interferon-gamma