Isometric contractile function following nerve grafting: a study of graft storage.
In order to assess the effects of storage on nerve grafts, the isometric contractile function of the gastrocnemius muscle was assessed 14 months following sciatic nerve autografting in the rat. Three-centimeter sciatic nerve grafts were stored at either 5 degrees C or 22 degrees C for 6 h, 24 h, or 3 weeks in an organ transplant solution. Muscle mass and maximal force in the fresh control graft group returned to 47% and 36% of normal levels, respectively, which was similar to stored grafts. Storage at 5 degrees C was superior to 22 degrees C and there was no decrement in contractile function in grafts stored up to 3 weeks at 5 degrees C. These findings suggest that the storage of nerve grafts is a feasible technique that might be applied to nerve allografts, thus permitting elective reconstruction of large peripheral nerve gaps.
Evans
P J
PJ
Department of Surgery, University of Toronto, Ontario, Canada.
Awerbuck
D C
DC
Mackinnon
S E
SE
Wade
J A
JA
McKee
N H
NH
D000704:Analysis of Variance; D000818:Animals; D004558:Electric Stimulation; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D007537:Isometric Contraction; D008297:Male; D009132:Muscles / Q000294:innervation*; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D012016:Reference Values; D012584:Sciatic Nerve / Q000502:physiology / Q000637:transplantation*; D013997:Time Factors; D014182:Transplantation, Autologous