Cisplatin blocks voltage-dependent calcium current in dissociated outer hair cells of guinea-pig cochlea.
The effect of the ototoxic molecule cisplatin (cis-DDP) on the voltage-dependent Ca2+ channel in dissociated outer hair cells (OHCs) of guinea-pig cochlea was investigated using a whole-cell patch-clamp technique. Cis-DDP had antagonistic effect on the Ca2+ channel and reversibly suppressed the Ca2+ current in a concentration-dependent manner. These results suggested that one of the ototoxic mechanisms of cis-DDP is involved in the inhibition of the Ca2+ channel in OHCs.
Yamamoto
T
T
Department of physiology, Kyushu University Faculty of Medicine, Fukuoka, Japan.
Kakehata
S
S
Saito
T
T
Saito
H
H
Akaike
N
N
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology*; D003051:Cochlea / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000494:pharmacology
D002121:Calcium Channel Blockers; D002945:Cisplatin; D011188:Potassium