Amniotic fluid enhances allogeneic cytotoxic T cell responses, whereas it suppresses mitogen-stimulated lymphocyte proliferation.
Mouse amniotic fluid has been shown to suppress T lymphocyte proliferation and suggested to be important in regulating immunity during pregnancy. In allogeneic pregnancy, cytotoxic T cells in pregnant lymphocytes against paternal transplantation antigen are impaired. We examined the effect of amniotic fluid to the alloreactive CTL responses. Although the amniotic fluid suppressed Con A or LPS stimulated lymphocyte proliferation as previously reported, the amniotic fluid taken from syngeneic C57BL/6 pregnant mice or allogeneic C57BL/6 x BALB/c pregnant mice enhanced the anti-H-2d or anti-H-2k CTL responses dose-dependently. We speculate that amniotic fluid contains not only immunosuppressive factors but also immunoenhancing factors which upregulate the allogeneic CTL responses.
Parvin
M
M
Department of Immunology, Nagoya University School of Medicine, Aichi, Japan.
Isobe
K
K
Zohreh
H
H
Goto
S
S
Nakashima
I
I
Tomoda
Y
Y
D000653:Amniotic Fluid / Q000276:immunology*; D000818:Animals; D002455:Cell Division; D003208:Concanavalin A; D005260:Female; D006183:H-2 Antigens / Q000276:immunology; D007519:Isoantigens / Q000276:immunology*; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D006183:H-2 Antigens; D007519:Isoantigens; D008070:Lipopolysaccharides; D003208:Concanavalin A