title: Protein tyrosine kinases and TNF alpha secretion in human monocytes.
abstract: The role of protein tyrosine kinases (PTK) in TNF alpha secretion by human monocytes was investigated in this report. We showed that an immunomodulator such as Nocardia lysozyme digest (NLD) and a particulate agonist, zymosan, stimulated an increase in tyrosine phosphorylation of several endogenous substrates including 53-56 kDa protein which was the predominant phosphoprotein. In addition, NLD and zymosan induced TNF alpha secretion which was impaired by a PTK inhibitor, tyrphostin. We suggest that a cascade of kinases including PTK is involved in NLD and zymosan signalling.
authors.affiliation: Laboratoire d'Immunologie, INSERM U 387, Hôpital de Sainte-Marguerite, Marseille, France.
authors.forename: H
authors.initials: H
authors.lastname: Lepidi
authors.forename: R
authors.initials: R
authors.lastname: Ciubotariu
authors.forename: P
authors.initials: P
authors.lastname: Bongrand
authors.forename: C
authors.initials: C
authors.lastname: Capo
authors.forename: R
authors.initials: R
authors.lastname: Barot-Ciorbaru
authors.forename: J L
authors.initials: JL
authors.lastname: Mege
mesh_terms: D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D009113:Muramidase / Q000494:pharmacology; D009615:Nocardia / Q000276:immunology; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology
chemical_list: D014409:Tumor Necrosis Factor-alpha; D014443:Tyrosine; D015054:Zymosan; D011505:Protein-Tyrosine Kinases; D009113:Muramidase