title: Immunoglobulin-type domains of titin are stabilized by amino-terminal extension.
abstract: We have recently suggested that similarly folded titin modules located at different sarcomeric regions have distinct molecular properties and stability. Could our selection of module boundaries have potentially influenced our conclusions? To address this question we expressed amino-terminally extended versions of the same modules and determined, with the use of CD and Fluorescence techniques, key thermodynamic parameters characterizing their stability. We present here our results which confirm our previous observations and show that, while amino-terminal extension has a profound effect on the stability of individual modules, it does not affect at all their folding pattern or their relative stabilities. Moreover, our data suggest that the selection of module boundaries can be of critical importance for the structural analysis of modular proteins in general, especially when a well-defined intron-exon topography is absent and proteolytic methods are inconclusive.
authors.affiliation: European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: A S
authors.initials: AS
authors.lastname: Politou
authors.forename: M
authors.initials: M
authors.lastname: Gautel
authors.forename: C
authors.initials: C
authors.lastname: Joseph
authors.forename: A
authors.initials: A
authors.lastname: Pastore
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D064211:Connectin; D017124:Conserved Sequence; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000737:chemistry; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry / Q000502:physiology*; D011489:Protein Denaturation; D017510:Protein Folding; D011494:Protein Kinases; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013816:Thermodynamics
chemical_list: D064211:Connectin; D007136:Immunoglobulins; D009124:Muscle Proteins; D009842:Oligopeptides; D011494:Protein Kinases