Brainstem neurons with projecting axons to both phrenic and abdominal motor nuclei: a double fluorescent labeling study in the cat.
The distribution of retrogradely doubly labeled brainstem neurons were analyzed in the cat after injection of two different fluorescent markers into the phrenic and abdominal motor nuclei. Diamidino Yellow (DY) was first injected either ipsilaterally or bilaterally into the ventral horn of lumbar spinal cord, and then Fast Blue (FB) into the right ventral horn of cervical spinal cord. Doubly labeled neurons were mainly found in the caudal ventrolateral medulla (retroambiguus region), in the dorsomedial and dorsolateral regions of the nucleus of the tractus solitarius (NTS) and in the raphe nuclei. In addition, doubly labeled neurons were found in the parabrachial and Kölliker-Fuse nuclei. Our results give anatomical evidence that pontine and medullary neurons are the source of a common pathway to both phrenic and abdominal motor nuclei. These neurons might be involved in strain efforts for expulsion such as vomiting or defecation.
Portillo
F
F
Département de Physiologie et Neurophysiologie, Faculté des Sciences et Techniques Saint Jérôme, Marseille, France.
Grélot
L
L
Milano
S
S
Bianchi
A L
AL
D000009:Abdominal Muscles / Q000294:innervation / Q000502:physiology*; D000578:Amidines; D000818:Animals; D001369:Axons / Q000502:physiology*; D001933:Brain Stem / Q000166:cytology / Q000502:physiology*; D002415:Cats; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D008297:Male; D009046:Motor Neurons / Q000166:cytology / Q000502:physiology*; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D010791:Phrenic Nerve / Q000166:cytology / Q000502:physiology*
D000578:Amidines; D005456:Fluorescent Dyes; C031455:diamidino compound 253-50; C037945:diamidino yellow