Molecular cloning and characterization of a cDNA showing tumor-suppressive activity in V-SRC-transformed 3Y1 rat fibroblasts.
Differential screening procedure was employed to isolate new genes specifically down-regulated in RSV-transformed rat 3Y1 cells (SR-3Y1) compared with parental 3Y1 cells. Among those, one clone termed N03 showed nearly complete down-regulation in v-src-, v-mos and SV40-transformed 3Y1 cells. Nucleotide sequence analysis showed that N03 protein is novel and is composed of 178 amino acid residues in length. Introduction of N03 cDNA into SR-3Y1 cells using an expression vector resulted in suppression of transformed phenotypes such as high growth rate, colony-forming ability in soft agar and tumorigenicity in nude mice.
Sakiyama
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Division of Biochemistry, Chiba Cancer Center Research Institute, Japan.
Ozaki
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Enomoto
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H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D016391:Genes, src / Q000235:genetics; D006801:Humans; D008168:Lung / Q000737:chemistry; D008175:Lung Neoplasms / Q000737:chemistry; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C490087:NBL1 protein, human; C085763:Nbl1 protein, rat; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger