title: A histological comparison of periapical inflammatory and neural responses to two endodontic sealers in the ferret.
abstract: Previous studies have shown that there is a proliferation of nerves beneath the apices of pulpectomized teeth. This may be due to the inflammation induced after the procedure, resulting, perhaps, from the irritant nature of the materials used to fill the root canal. The experiment reported here was conducted to determine whether this inflammation was induced by the sealer rather than arising as a result of tissue damage and whether, if inflammation is eliminated or reduced, the neural changes are also reduced. In 12 young adult ferrets under general anaesthesia the pulps of the lower canine teeth were removed and replaced with gutta percha and sealer. A sealer of Grossman's formulation with eugenol as the liquid phase was used on one side and a calcium hydroxide sealer that contained no eugenol on the other. Three months later the animals were, again under general anaesthesia, perfused with a fixative mixture. The mandibles were removed and the presence and size of any periapical inflammatory lesions and the density of periapical innervation determined histologically. All 12 teeth sealed with the Grossman's sealer had inflammatory lesions at their apices. Three of the 12 teeth treated with the calcium hydroxide sealer showed similar lesions. In all the teeth with inflammatory lesions the normal arrangement of nerves in a periodontal 'plexus' was disrupted but there was no statistically significant difference between the overall innervation density in inflamed and non-inflamed periapical areas nor between areas beneath teeth sealed with Grossman's sealer and with calcium hydroxide. The incidence of periapical inflammation is related to the nature of endodontic sealer used.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, University of Alberta, Canada.
authors.forename: G R
authors.initials: GR
authors.lastname: Holland
mesh_terms: D000818:Animals; D002126:Calcium Hydroxide / Q000633:toxicity; D005289:Ferrets; D006171:Gutta-Percha; D009412:Nerve Fibers / Q000187:drug effects*; D010485:Periapical Periodontitis / Q000139:chemically induced* / Q000473:pathology; D010486:Periapical Tissue / Q000187:drug effects / Q000294:innervation* / Q000473:pathology; D011670:Pulpectomy; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012459:Salicylates; D016037:Single-Blind Method; D018709:Statistics, Nonparametric; D014092:Tooth Root / Q000187:drug effects / Q000294:innervation / Q000473:pathology; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity*
chemical_list: D012387:Root Canal Filling Materials; D012459:Salicylates; D015035:Zinc Oxide-Eugenol Cement; C045432:Grossman's sealer; C055601:Sealapex; D006171:Gutta-Percha; D002126:Calcium Hydroxide