title: Carba prostacyclin enhances the capsaicin-induced cobalt-loading of rat sensory neurons grown in culture.
abstract: Sensory neurons isolated from the dorsal root ganglia of embryonic rats and grown in culture are distributed into two populations based on cross-sectional areas and sensitivity to capsaicin. Of the total neuronal population, about 48% of the neurons are labelled by cobalt in the presence of 1 microM capsaicin. The distribution of the small-sized neurons coincides with that of the capsaicin-cobalt-labelled neurons. When a lower concentration of capsaicin (100 nM) is used, pretreatment of sensory neurons with carba prostacyclin, a stable analog of prostaglandin I2, produces a three-fold enhancement in the number of neurons that are cobalt-labelled by capsaicin compared with that in the absence of carba prostacyclin.
authors.affiliation: Department of Pharmacology and Toxicology, School of Medicine, Indiana University, Indianapolis 46202-5120.
authors.forename: C M
authors.initials: CM
authors.lastname: Hingtgen
authors.forename: G D
authors.initials: GD
authors.lastname: Nicol
mesh_terms: D000818:Animals; D002211:Capsaicin / Q000494:pharmacology*; D002478:Cells, Cultured; D003035:Cobalt / Q000493:pharmacokinetics / Q000494:pharmacology*; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D009475:Neurons, Afferent / Q000187:drug effects* / Q000378:metabolism; D011465:Prostaglandins, Synthetic / Q000494:pharmacology*; D051381:Rats
chemical_list: D011465:Prostaglandins, Synthetic; D003035:Cobalt; C026678:carboprostacyclin; D011464:Epoprostenol; D002211:Capsaicin