Mitomycin C induced alterations in antioxidant enzyme levels in a model insect species, Spodoptera eridania.
1. An insect species, the southern armyworm Spodoptera eridania, was used as an in vivo model to examine mitomycin C's (MMC) pro-oxidant effect reflected in alterations of antioxidant enzymes. 2. Following a 2-day exposure to 0.01 and 0.05% w/w dietary concentrations, MMC only induced superoxide dismutase activity. All other enzyme activities were not affected, indicating oxidative stress was mild. 3. Following a 5-day exposure to 0.05% w/w dietary MMC, the activities of superoxide dismutase, glutathione-S-transferase and its peroxidase activity and DT-diaphorase were induced. GR activity was not altered. The high constitutive catalase activity was also not affected. These responses of S. eridania's antioxidant enzymes are analogous to those of mammalian systems in alleviating MMC-induced oxidative stress. 4. S. eridania emerges as an appropriate non-mammalian model for initial and cost-effective screening of drug-induced oxidative stress.
Batcabe
J P
JP
Department of Biochemistry/330, University of Nevada, Reno 89557-0014.
MacGill
R S
RS
Zaman
K
K
Ahmad
S
S
Pardini
R S
RS
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D005982:Glutathione Transferase / Q000187:drug effects; D016685:Mitomycin / Q000494:pharmacology*; D008954:Models, Biological; D015394:Molecular Structure; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D018411:Spodoptera / Q000187:drug effects* / Q000201:enzymology; D013482:Superoxide Dismutase / Q000187:drug effects
D000975:Antioxidants; D016685:Mitomycin; D013482:Superoxide Dismutase; D016660:NAD(P)H Dehydrogenase (Quinone); D005982:Glutathione Transferase