Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen.
Cloning of a cDNA from Cry j II, the second major allergen from Japanese cedar (Cryptomeria japonica) pollen, is described. An isolated Cry j II cDNA contained an open reading frame coding for 514 amino acid residues. The mature Cry j II protein consisted of 388 amino acid residues (R46-S433). According to a homology analysis, no amino acid sequence homology was observed between Cry j II and Cry j I, another major allergen. But Cry j II showed homology with polygalacturonase (PG) derived from tomato (40% identity) at the amino acid level. The sequence information can potentially be used to devise an effective course of immunotherapy for Japanese cedar pollinosis.
Namba
M
M
Fujisaki Institute, Hayashibara Biochemical Laboratories Inc., Okayama, Japan.
Kurose
M
M
Torigoe
K
K
Hino
K
K
Taniguchi
Y
Y
Fukuda
S
S
Usui
M
M
Kurimoto
M
M
D000485:Allergens / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011058:Pollen; D011096:Polygalacturonase / Q000737:chemistry; D017385:Sequence Homology
D000485:Allergens; C065291:Cry j II protein, Cryptomeria japonica; D018076:DNA, Complementary; D010940:Plant Proteins; D011096:Polygalacturonase