Resistance to degradation by nucleases of (2'S)-2'-deoxy-2'-C-methyloligonucleotides, novel potential antisense probes.
An oligonucleotide must fulfill two main requirements to become a potential antisense probe: effective hybridization properties with the complementary sequence and stability toward nucleases. In this article the degradation pattern of a new class of potential antisense fragment, (2'S)-2'-deoxy-2'-C-methyloligonucleotides, is analyzed. The results described here show that the modification introduced in these oligonucleotides confers an enhanced stability toward purified nucleases, human sera, and HeLa cell extracts.
Iribarren
A M
AM
Istituto di Richerche di Biologia Molecolare P. Angeletti, Pomezia, Rome, Italy.
Cicero
D O
DO
Neuner
P J
PJ
D001483:Base Sequence; D003851:Deoxyribonucleases / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry*
D016376:Oligonucleotides, Antisense; D003851:Deoxyribonucleases; D012260:Ribonucleases