Characterization of a predominantly pistil-expressed gene encoding a gamma-thionin-like protein of Petunia inflata.
We isolated a cDNA clone from a pistil cDNA library of Petunia inflata which encodes a protein, PPT, with sequence similarity to gamma-thionins. Characterization of a genomic clone containing a PPT gene revealed the presence of a single intron. Northern analysis revealed that the PPT gene was predominantly expressed in the pistil during all stages of flower development. Since thionins have been implicated in plant defense against pathogens, PPT may play a role similar to that of other defense-related proteins found in the pistil, defending the pistil against pathogen infection.
Karunanandaa
B
B
Graduate Program in Plant Physiology, Pennsylvania State University, University Park 16802.
Singh
A
A
Kao
T H
TH
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D018547:Plant Stems / Q000737:chemistry; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C090429:PPT protein, Petunia inflata; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant