title: Expression of MUC2-mucin in colorectal adenomas and carcinomas of different histological types.
abstract: The expression of mucin MUC2 was investigated in normal colonic tissue, in colonic adenomas and in carcinomas of the mucinous and non-mucinous type. The latter were subdivided into carcinomas originating from the adenoma-carcinoma sequence (ACS) and de novo (DN) carcinomas. The expression was assayed by immunohistochemistry with the monoclonal anti-MUC2 antibody CCP58 and by mRNA semiquantitation. MUC2 protein epitope CCP58 was strongly expressed in 21% of normal colonic tissues, in 40% of villous and in 48% of tubular adenomas. Mucinous carcinomas exhibited strong expression in 72%, ACS carcinomas in 21% and DN adenocarcinomas in none of the tumors investigated. Compared with the adjacent non-malignant tissue (transitional mucosa), CCP58 epitope expression in the tumor was higher in 74% of mucinous carcinomas, but equal or lower in 69% of ACS carcinomas and in 100% of de novo carcinomas. The alterations of MUC2 expression detected by immunohistochemistry in adenocarcinomas were confirmed on mRNA level. These data indicate that the MUC2 expression pattern is different in the 3 carcinoma types investigated. MUC2 over-expression occurs in the adenomatous tissue. It is always maintained in mucinous carcinomas, but frequently decreased in non-mucinous ACS carcinomas. DN carcinomas are most frequently associated with decreased expression of MUC2.
authors.affiliation: Klinikum Steglitz, Freien Universität Berlin, Department of Gastroenterology, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Blank
authors.forename: E
authors.initials: E
authors.lastname: Klussmann
authors.forename: S
authors.initials: S
authors.lastname: Krüger-Krasagakes
authors.forename: A
authors.initials: A
authors.lastname: Schmitt-Gräff
authors.forename: M
authors.initials: M
authors.lastname: Stolte
authors.forename: G
authors.initials: G
authors.lastname: Bornhoeft
authors.forename: H
authors.initials: H
authors.lastname: Stein
authors.forename: P X
authors.initials: PX
authors.lastname: Xing
authors.forename: I F
authors.initials: IF
authors.lastname: McKenzie
authors.forename: C P
authors.initials: CP
authors.lastname: Verstijnen
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000378:metabolism* / Q000473:pathology; D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D018253:Adenoma, Villous / Q000378:metabolism / Q000473:pathology; D018256:Adenomatous Polyps / Q000378:metabolism / Q000473:pathology; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D017931:DNA Primers; D003937:Diagnosis, Differential; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D055262:Mucin-2; D009077:Mucins / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D017931:DNA Primers; C524625:MUC2 protein, human; D055262:Mucin-2; D009077:Mucins; D009363:Neoplasm Proteins; D012333:RNA, Messenger