title: Effect of infrared analyzer homogenization efficiency on repeatability of uncorrected fat A and fat B signals.
abstract: Poor repeatability by infrared milk analyzers may be caused by inefficient homogenization as a result of light scattering and the Christiansen effect. The objectives of this study were to identify instruments with good and poor homogenization efficiency and to determine if a difference exists in repeatability performance between instruments with good vs poor homogenization efficiency. Unhomogenized and homogenized portions of the same milk were tested 20 times consecutively on 22 instruments. An instrument was considered to have poor homogenization efficiency if the mean difference in the uncorrected signal between unhomogenized and homogenized portions of the same milk was > or = 1.43% of the fat test (i.e., > or = 0.05% at 3.5% fat). Instruments were evaluated for repeatability by calculating the sample standard deviation and the range of the latter 19 uncorrected readings for unhomogenized and homogenized milks. When repeatability was evaluated as a function of homogenization efficiency, there was a significant (p = 0.001) correlation between poor homogenization efficiency and poor repeatability when testing unhomogenized milk but not when testing homogenized milk. Improved homogenizer performance within infrared milk analyzers is needed to improve the repeatability of raw milk testing.
authors.affiliation: Cornell University, Northeast Dairy Foods Research Center, Ithaca, NY 14853.
authors.forename: E B
authors.initials: EB
authors.lastname: Smith
authors.forename: D M
authors.initials: DM
authors.lastname: Barbano
authors.forename: J M
authors.initials: JM
authors.lastname: Lynch
authors.forename: J R
authors.initials: JR
authors.lastname: Fleming
mesh_terms: D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000706:statistics & numerical data; D007259:Infrared Rays; D008055:Lipids / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012542:Scattering, Radiation
chemical_list: D008055:Lipids