title: Apoptosis induced in mouse hepatitis virus-infected cells by a virus-specific CD8+ cytotoxic T-lymphocyte clone.
abstract: Morphological changes of mouse hepatitis virus-infected J774.1 cells cocultured with cloned mouse hepatitis virus-specific CD8+ cytotoxic T lymphocytes were examined by electron microscopy. Condensation and margination of chromatin, cellular shrinkage with severe vacuolar degeneration, and blebbing were observed. In addition, fragmentation of cellular DNA was observed, and a decrease in virus titer was accompanied by those changes. These findings show that the cloned cytotoxic T lymphocytes induce in the target cell an internal degradation program termed apoptosis, which results in virus clearance.
authors.affiliation: Laboratory of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shibata
authors.forename: S
authors.initials: S
authors.lastname: Kyuwa
authors.forename: S K
authors.initials: SK
authors.lastname: Lee
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
authors.forename: N
authors.initials: N
authors.lastname: Goto
mesh_terms: D000818:Animals; D017209:Apoptosis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D002448:Cell Adhesion; D002478:Cells, Cultured; D002999:Clone Cells; D004247:DNA / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D006517:Murine hepatitis virus / Q000276:immunology* / Q000472:pathogenicity; D014779:Virus Replication
chemical_list: D004247:DNA