title: Dynamic structures of intact chicken erythrocyte chromatins as studied by 1H-31P cross-polarization NMR.
abstract: The dynamic properties of DNA in intact chicken erythrocyte cells, nuclei, nondigested chromatins, digested soluble chromatins, H1, H5-depleted soluble chromatins and nucleosome cores were investigated by means of single-pulse and 1H-31P cross-polarization NMR. The temperature dependence of the phosphorus chemical shift anisotropy was identical for the former three in the presence of 3 mM MgCl2, suggesting that the local higher order structure is identical for these chromatins. The intrinsic phosphorus chemical shift anisotropy of the nucleosome cores was -159 ppm. The chemical shift anisotropy of DNA in the chromatins can be further averaged by the motion of the linker DNA. The spin-lattice relaxation time in the rotating frame of the proton spins (T1p) of the nondigested chromatins was measured at various locking fields. The result was analyzed on the assumption of the isotropic motion to get a rough value of the correlation time of the motion efficient for the relaxation, which was eventually ascribed to the segmental motion of the linker DNA with restricted amplitude. The 30 nm filament structure induced by NaCl was shown to be dynamically different from that induced by MgCl2. Side-by-side compaction of 30-nm filaments was suggested to be induced in the MgCl2 concentration range higher than 0.3 mM. Biological significance of the dynamic structure was discussed in connection with the results obtained.
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Yokohama National University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Akutsu
authors.forename: S
authors.initials: S
authors.lastname: Nishimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kyogoku
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D002645:Chickens; D002843:Chromatin / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis*; D004912:Erythrocytes / Q000737:chemistry / Q000648:ultrastructure*; D006859:Hydrogen; D015636:Magnesium Chloride / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009994:Osmolar Concentration; D010758:Phosphorus; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D002843:Chromatin; D015636:Magnesium Chloride; D010758:Phosphorus; D012965:Sodium Chloride; D006859:Hydrogen; D004247:DNA