Effect of trimebutine maleate on the contractile response of the isolated ileum from diabetic rats.
1. Tension of the isolated ileum from diabetic rats induced by streptozotocin was measured isometrically to study the mode of action of trimebutine maleate (TMB). 2. The hyperreactivity of contractile response to KCl was observed in the isolated ileum from diabetic rats. TMB inhibited the contraction induced by KCl and acetylcholine (ACh) in normal solution. 3. In Ca2+ free solution, the hyperreactivity of contractile response to KCl was attenuated, and TMB did not inhibit the contraction induced by KCl. In contrast, TMB inhibited the contraction induced by ACh even in Ca2+ free solution. 4. These results suggest that the hyperreactivity of contractile response to KCl in the ileum from diabetic rats is due to the enhancement of Ca2+ influx through voltage-dependent Ca2+ channel and that TMB inhibits the hyperreactivity of contractile response through the inhibition of Ca2+ movement by the cell.
Uchida
M
M
Pharmacological Laboratories, Grelan Pharmaceutical Co. Ltd., Tokyo, Japan.
Iwata
T
T
Takagi
S
S
Sugiyama
Y
Y
Ishitani
K
K
Honda
H
H
Sakai
Y
Y
D000818:Animals; D002118:Calcium / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000503:physiopathology*; D004353:Drug Evaluation, Preclinical; D005767:Gastrointestinal Diseases / Q000188:drug therapy; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions; D014287:Trimebutine / Q000494:pharmacology*
D012996:Solutions; D014287:Trimebutine; D002118:Calcium