Primary central nervous system malignant non-Hodgkin's lymphomas from HIV-infected and non-infected patients: expression of cellular surface proteins and Epstein-Barr viral markers.
The increased incidence of primary central nervous system malignant non-Hodgkin's lymphomas (PCNSL) in HIV- and non-HIV-infected patients and the demonstration of Epstein-Barr virus (EBV) in these tumours may indicate relationships between PCNSL and EBV. Consequently expression of EBV-induced antigens and cellular markers were studied in 11 HIV-infected and seven non-infected patients by in situ hybridization (ISH) and immunocytochemistry in monoclonal B cell PCNSL. In HIV-infected patients EBV genome was present in 9/11 cases, LMP in 11/11 cases and EBNA2 in 10/11 cases. The expression of adhesion and activation molecules was low or absent. In HIV non-infected patients, EBV genome was present in 5/7 cases, with LMP in 4/7 cases. EBNA2 was never detected. All these lymphomas expressed LFA1beta. Whatever the population, no lytic cycle EBV markers were detected. Compared with other types of EBV lymphomas, our results suggest a different EBV latency state in primary B cell lymphomas of the CNS from HIV-infected or non-infected patients.
Auperin
I
I
Department of Bacteriology and Virology, Saint-Louis Hospital, Paris VII University, France.
Mikolt
J
J
Oksenhendler
E
E
Thiebaut
J B
JB
Brunet
M
M
Dupont
B
B
Morinet
F
F
D000328:Adult; D000368:Aged; D015415:Biomarkers; D001932:Brain Neoplasms / Q000150:complications / Q000378:metabolism*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis / Q000276:immunology; D003001:Cloning, Molecular; D005260:Female; D015700:HIV Envelope Protein gp41 / Q000096:biosynthesis / Q000276:immunology; D015658:HIV Infections / Q000150:complications / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D016483:Lymphoma, AIDS-Related / Q000150:complications / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000150:complications / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008875:Middle Aged
D015415:Biomarkers; D015816:Cell Adhesion Molecules, Neuronal; D015700:HIV Envelope Protein gp41; D008565:Membrane Proteins