title: Modulation of phenotypic and functional properties of normal human mononuclear phagocytes by granulocyte-macrophage colony-stimulating factor.
abstract: In asthma, alveolar macrophages (AMs) are hyperreactive releasing large amounts of mediators and expressing high levels of surface markers. Granulocyte-macrophage colony-stimulating factor (GM-CSF) upregulates monocytes and AMs, and may be involved in the hyperreactivity of AMs. The effects of GM-CSF were tested on monocytes and AMs from normal subjects by examining the expression of factors thought to be upregulated in asthma. After various incubation times of GM-CSF, the expression of CD23 and beta 2-integrins (CD11a, CD11b, CD11c) was studied by FACS and the release of sCD23 was measured by ELISA. The priming and stimulatory effects of GM-CSF were tested on monocytes and AMs and the release of leukotriene B4 (LTB4) was measured by ELISA. GM-CSF induced the expression of CD11c and CD23 and the release of sCD23. GM-CSF primed and stimulated monocytes and AMs to release LTB4. The effects of GM-CSF may explain partly the hyperreactivity of AMs in asthma.
authors.affiliation: CJF INSERM 92-10, Hôpital Arnaud-de-Villeneuve, Montpellier, France.
authors.forename: A
authors.initials: A
authors.lastname: Rivier
authors.forename: P
authors.initials: P
authors.lastname: Chanez
authors.forename: J
authors.initials: J
authors.lastname: Pène
authors.forename: F B
authors.initials: FB
authors.lastname: Michel
authors.forename: P
authors.initials: P
authors.lastname: Godard
authors.forename: B
authors.initials: B
authors.lastname: Dugas
authors.forename: J
authors.initials: J
authors.lastname: Bousquet
mesh_terms: D000328:Adult; D000368:Aged; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002478:Cells, Cultured; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology*; D006801:Humans; D016130:Immunophenotyping; D016023:Integrins / Q000096:biosynthesis; D007975:Leukotriene B4 / Q000096:biosynthesis; D016676:Macrophages, Alveolar / Q000276:immunology*; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D017455:Receptors, IgE / Q000096:biosynthesis
chemical_list: D015815:Cell Adhesion Molecules; D016023:Integrins; D017455:Receptors, IgE; D007975:Leukotriene B4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor