Evidence against involvement of APC mutation in papillary thyroid carcinoma.
Papillary thyroid carcinoma (PTC) is one of several tumours associated with familial adenomatous polyposis (FAP), an inherited tumour syndrome which appears to result from germ-line mutation of the APC tumour suppressor gene. Here we investigate the possibility that somatic mutation of APC might play a role in sporadic PTC. 16 cases of PTC together with matched normal tissue were examined by single-strand conformation polymorphism (SSCP) analysis, concentrating on the mutation cluster region (MCR) of the APC gene (codons 1286-1513). No evidence of mutation was observed in any sample. We conclude that APC mutation, at least in the MCR, is not a significant causal mechanism in sporadic PTC.
Curtis
L
L
Department of Pathology, University of Edinburgh, U.K.
Wyllie
A H
AH
Shaw
J J
JJ
Williams
G T
GT
Radulescu
A
A
DeMicco
C
C
Haugen
D R
DR
Varhaug
J E
JE
Lillehaug
J R
JR
Wynford-Thomas
D
D
D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D002291:Carcinoma, Papillary / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D005784:Gene Amplification; D017491:Genes, APC / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013964:Thyroid Neoplasms / Q000235:genetics*