Inhibition, tissue distribution and hormonal specificity of a promoter-binding factor for the uteroglobin gene.
We had earlier reported a uteroglobin promoter-binding factor in nuclei from progesterone-stimulated rabbit endometrium that was inhibited by a factor in nuclei without progesterone stimulation or from non-target tissues (Rider, V., and Bullock, D.W., 1988. Biochem. Biophys. Res. Comm. 156, 1368-1375). In the course of purification of the inhibitory activity, the effect was shown to be due to contaminating genomic DNA. The inhibitor was destroyed by treatment with DNase I and resisted phenol-chloroform extraction. Fractionation of nuclear extracts on columns of DEAE-Sepharose separated the inhibitor and revealed the presence of binding activity in unstimulated or estrogen-treated endometrium, as well as in liver, lung and ovary. The tissue and hormonal specificity of the promoter binding factor is thus less restricted than recently reported.
Daniel
P B
PB
Centre for Molecular Biology, Lincoln University, Canterbury, New Zealand.
Molloy
C
C
Rayner
J C
JC
Hill
A M
AM
Bullock
D W
DW
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D011817:Rabbits; D011980:Receptors, Progesterone / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D014598:Uteroglobin / Q000235:genetics*
D004268:DNA-Binding Proteins; D011980:Receptors, Progesterone; D011374:Progesterone; D004958:Estradiol; D014598:Uteroglobin; D003850:Deoxyribonuclease I