cDNA cloning and expression of bovine endothelin converting enzyme.
We have cloned cDNA encoding bovine endothelin converting enzyme (ECE). The predicted amino acid sequence of bovine ECE consisted of 758 amino acid residues. Northern blot analysis revealed that ECE mRNA was abundantly expressed in lung. Co-expression of the cloned cDNA of bovine ECE with human preproET-1 cDNA in CHO-K1 cells resulted in the production of mature ET-1 and this production was inhibited by phosphoramidon.
Ikura
T
T
Department of Pharmacology, Faculty of Medicine, Kyoto University, Japan.
Sawamura
T
T
Shiraki
T
T
Hosokawa
H
H
Kido
T
T
Hoshikawa
H
H
Shimada
K
K
Tanzawa
K
K
Kobayashi
S
S
Miwa
S
S
D000595:Amino Acid Sequence; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D016466:CHO Cells; D002417:Cattle; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000378:metabolism; D019332:Endothelin-1; D000072396:Endothelin-Converting Enzymes; D016232:Endothelins / Q000096:biosynthesis / Q000378:metabolism; D015870:Gene Expression; D006020:Glycopeptides / Q000494:pharmacology; D006801:Humans; D008168:Lung / Q000201:enzymology*; D008666:Metalloendopeptidases; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000096:biosynthesis / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D014162:Transfection
D018076:DNA, Complementary; D019332:Endothelin-1; D016232:Endothelins; D006020:Glycopeptides; D011498:Protein Precursors; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; D000072396:Endothelin-Converting Enzymes; C008890:phosphoramidon