title: PCR cloning using T-vectors.
authors.affiliation: Molecular Genetics Unit, MRC Centre, Cambridge, UK.
authors.forename: M K
authors.initials: MK
authors.lastname: Trower
authors.forename: G S
authors.initials: GS
authors.lastname: Elgar
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D019914:Taq Polymerase; D004253:DNA Nucleotidylexotransferase; D004259:DNA-Directed DNA Polymerase