Tritium isotope effect in high-performance liquid chromatography of eicosanoids.
A significant difference in retention time between unlabeled and the corresponding multi-tritium-labeled eicosanoid has been observed in the high performance liquid chromatography (HPLC) analysis of 11 eicosanoids. Variations in retention time range from 3-7%, depending on the separation conditions as well as the number and position of the tritium substitution. Multi-tritium-labeled eicosanoids were eluted earlier than the corresponding unlabeled eicosanoid in reversed phase HPLC, whereas no isotope effect was seen with 14C- and 3H2-eicosanoids. Considerations must be given to this tritium isotope effect whenever both multi-tritium-labeled and unlabeled eicosanoids are used for HPLC cochromatography or recovery studies.
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Lipids Group, DuPont, NEN Products, Boston, MA 02118.
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Iles
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Rosenberger
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Hong
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Ahern
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D002851:Chromatography, High Pressure Liquid; D015777:Eicosanoids / Q000032:analysis*; D006859:Hydrogen; D015394:Molecular Structure; D014316:Tritium / Q000737:chemistry
D015777:Eicosanoids; D014316:Tritium; D006859:Hydrogen