Characterization of cDNAs species encoding the Tat protein of caprine arthritis encephalitis virus.
Two distinct species of caprine arthritis encephalitis virus (CAEV) tat cDNAs were isolated early after infection of a Himalayan tahr cell line. Sequence analyses predicted that one cDNA (pCEV/e1) represented a polycistronic transcript that encodes Tat and Rev as well as an N-terminally truncated transmembrane protein and a protein, designated X, whose function is unknown; whereas the other cDNA (pCEV/f1) encodes Tat and the env gene products. pCEV/e1 trans-activated a CAEV LTR-chloramphenicol acetyltransferase reporter gene in goat synovial membrane cells. This activity was shown to be encoded by the Tat open reading frame by analysis of a deletion mutant. Because the pCAEV/f1 insert was unstable in plasmid form, its Tat activity could not be convincingly demonstrated. The target sequences for Tat within the CAEV LTR were localized to the U3 region which, when placed in either orientation upstream of heterologous promoters, was able to confer responsiveness to Tat.
Kalinski
H
H
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Israel.
Mashiah
P
P
Rotem
D
D
Orzech
Y
Y
Sherman
L
L
Miki
T
T
Yaniv
A
A
Gazit
A
A
Tronick
S R
SR
D000595:Amino Acid Sequence; D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D015696:Gene Products, tat / Q000737:chemistry / Q000235:genetics*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D015533:Transcriptional Activation
D018076:DNA, Complementary; D015696:Gene Products, tat