C-type natriuretic peptide increases cyclic GMP in rat cerebral microvessels in primary culture.
Effect of CNP on cGMP level in cultured rat cerebral microvessels was investigated. The cerebral microvessels were prepared from rat cerebral cortex by dispase and collagenase digestion and Percoll gradient centrifugation, and cultured. CNP increased cGMP level in a dose-dependent manner suggesting that CNP has a regulatory role in the cerebral microvessel function.
Kobayashi
H
H
Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Ueno
S
S
Tsutsui
M
M
Okazaki
M
M
Uezono
Y
Y
Yanagihara
N
N
Yuhi
T
T
Izumi
F
F
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation / Q000502:physiology*; D006152:Cyclic GMP / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D020098:Natriuretic Peptide, C-Type; D011506:Proteins / Q000494:pharmacology*; D051381:Rats
D011506:Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP