title: Development of a predictive model for optimal zona pellucida binding using insemination volume and sperm concentration.
abstract: OBJECTIVE
  To develop a predictive model under hemizona assay (HZA) conditions for human spermatozoa concentrations and insemination volume for optimum zona pellucida (ZP) binding.
  
  
  METHODS
  Analysis of 20 different insemination volumes for zona binding and sperm morphology under HZA conditions.
  
  
  METHODS
  Reproductive biology unit, tertiary medical center.
  
  
  METHODS
  Four proven fertile sperm donors.
  
  
  METHODS
  5-, 20-, 50-, 80-, and 100-microL droplets were analyzed with four different concentrations of 0.5 x 10(6), 1.0 x 10(6), 2.0 x 10(6), and 4.0 x 10(6) cells/mL to determine the number of sperm bound to each hemizona. Fifteen hemizonae were used for each insemination volume or microdroplet. Response surface regression model with volume and concentration as the regressor variables has been used.
  
  
  RESULTS
  The response surface of binding for the factors concentration and volume showed nonlinear association. A formula, indicating the optimal sperm insemination volume for maximum sperm binding to the ZP, Vmax = -(b1 + b5c)/2b6c, is described. The transformed data indicated 60 microL containing 4 x 10(6) sperm/mL to be optimal. Although morphology of zona spermatozoa is superior compared with seminal and postswim-up samples, no difference among the percentage of the normal morphology in different microdroplets could be demonstrated.
  
  
  CONCLUSIONS
  Optimal volume for the obtained concentration of spermatozoa from a patient can be calculated and therapeutically used for cases of severe oligozoospermic patients by microvolume inseminations in IVF practice.
authors.affiliation: Department of Obstetrics and Gynaecology, Akdeniz University Medical Faculty, Antalya, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Ozgür
authors.forename: D R
authors.initials: DR
authors.lastname: Franken
authors.forename: K
authors.initials: K
authors.lastname: Kaskar
authors.forename: C J
authors.initials: CJ
authors.lastname: Lombard
authors.forename: T F
authors.initials: TF
authors.lastname: Kruger
mesh_terms: D005260:Female; D006801:Humans; D007315:Insemination, Artificial; D008297:Male; D008962:Models, Theoretical; D011237:Predictive Value of Tests; D012044:Regression Analysis; D013076:Sperm Count; D013094:Spermatozoa / Q000378:metabolism*; D015044:Zona Pellucida / Q000378:metabolism*