Relation between platinum-DNA adducts and complete remission in adult acute nonlymphocytic leukemia.
The purpose of this study was to determine the relationship between platinum-DNA adducts in leukemic cells and the success of remission induction therapy in adult patients with acute nonlymphocytic leukemia (ANLL). Freshly isolated cells from pre-treatment bone marrow aspirates of 14 patients were incubated with cisplatin in vitro and the amount of platinum bound to DNA was determined using a competitive ELISA procedure and an antibody directed against platinum-modified DNA. Platinum-DNA adduct levels discriminated well between complete remission (CR) and remission failure (RF) patients, treated with mitoxantrone, cytosine arabinoside +/- carboplatin.
Armstrong
S G
SG
Ontario Cancer Treatment and Research Foundation, Hamilton, Canada.
Browman
G P
GP
Benger
A M
AM
Meyer
R M
RM
McKay
K L
KL
Singh
G
G
D000293:Adolescent; D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002945:Cisplatin / Q000378:metabolism*; D003561:Cytarabine / Q000008:administration & dosage; D018736:DNA Adducts / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D008942:Mitoxantrone / Q000008:administration & dosage; D009944:Organoplatinum Compounds / Q000032:analysis* / Q000378:metabolism; D011237:Predictive Value of Tests; D012008:Recurrence; D012074:Remission Induction
C065354:1,2-cyclohexanediamine platinum(II)-DNA complex; D018736:DNA Adducts; D009944:Organoplatinum Compounds; D003561:Cytarabine; D008942:Mitoxantrone; D002945:Cisplatin