Choline acetyltransferase and NADPH-diaphorase localization in the goldfish habenulo-interpeduncular system.
The habenular nuclei are the major sources of projections to the interpeduncular nucleus. The habenular neurons of the goldfish are ChAT and NADPH-diaphorase positive. The localization of these two enzymes in the habenulae suggests the involvement of acetylcholine and nitric oxide (the product of NADPH-diaphorase activity in the nervous tissue) in the habenulo-interpeduncular connection. This finding is supported by the presence of ChAT and NADPH-diaphorase activity in the neuropilar area of the interpeduncular nucleus. This activity was depleted 12 days after habenular ablation. The overlap of ChAT and NADPH-diaphorase localization in goldfish habenular neurons is consistent with previous observations on the co-localization of these two enzymes in some brainstem neurons of other vertebrates. It is, however, not identical to the localization of NADPH-diaphorase in the habenulae and in the interpeduncular nucleus of the rat.
Villani
L
L
Department of Biology, University of Bologna, Italy.
Guarnieri
T
T
Zironi
I
I
D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000033:anatomy & histology / Q000201:enzymology*; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D013788:Thalamus / Q000033:anatomy & histology / Q000201:enzymology*
D009252:NADPH Dehydrogenase; D002795:Choline O-Acetyltransferase