[Foreign genes expression in Wistar rats in vivo].
pN2-LacZ or pN2-CMV-ProUK plasmid DNA was separately injected into rat quadricept muscles and beta-galactosidase or Pro-UK was detected after 2 days. The expression of foreign genes lasted for at least 3 months. pN2-LacZ plasmid was injected into the left ventricular wall of the rat, and the expression of LacZ gene was more efficient than in the quadricept muscle. pN2-LacZ was also introduced into the rat arterial wall by balloon catheter. A new method was also found to improve the efficiency of foreign gene expression in muscle by stitching medical stitch containing plasmid DNA onto the quadricept muscle.
Zhu
X J
XJ
Institute of Cardiovascular Research, Beijing Medical University.
Yao
A Q
AQ
Liu
B
B
D000818:Animals; D004792:Enzyme Precursors / Q000096:biosynthesis; D015870:Gene Expression; D018014:Gene Transfer Techniques; D008297:Male; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010957:Plasmids; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000096:biosynthesis; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000096:biosynthesis*
D004792:Enzyme Precursors; D011994:Recombinant Proteins; D001616:beta-Galactosidase; D014568:Urokinase-Type Plasminogen Activator; C067551:saruplase