Characterization of the principal colouring components of annatto using high performance liquid chromatography with photodiode-array detection.
The principal colouring components of the natural food colouring material annatto, the 9'-cis- and all-trans- isomers of bixin and norbixin, have been prepared in pure form. A reverse-phase HPLC method utilizing photodiode-array detection has been developed to enable their chromatographic and spectroscopic characterization. One minor component, a di-cis-isomer of norbixin has also been identified and characterized.
Scotter
M J
MJ
Ministry of Agriculture, Fisheries and Food, Food Science Laboratory, Colney, Norwich, UK.
Thorpe
S A
SA
Reynolds
S L
SL
Wilson
L A
LA
Strutt
P R
PR
D002338:Carotenoids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005505:Food Coloring Agents / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
D005505:Food Coloring Agents; D010936:Plant Extracts; D002338:Carotenoids