title: Crystallization and preliminary X-ray diffraction study of the nickel-binding protein NikA of Escherichia coli.
abstract: NikA, a periplasmic nickel-binding protein involved in nickel-repellent chemotaxis has been crystallized. The crystals are hexagonal with space group P6(2) (or its enantiomorph) with a = 160.3 A and c = 138.4 A and they diffract to at least 3.0 A resolution in the laboratory. NikA presents sequence homology with several periplasmic solute-binding proteins from Gram-negative bacteria.
authors.affiliation: Laboratoire de Cristallographie et Cristallogenèse des Protéines, Institut de Biologie Structurale J. P. Ebel CEA-CNRS, Grenoble, France.
authors.forename: M H
authors.initials: MH
authors.lastname: Charon
authors.forename: L F
authors.initials: LF
authors.lastname: Wu
authors.forename: C
authors.initials: C
authors.lastname: Piras
authors.forename: K
authors.initials: K
authors.lastname: de Pina
authors.forename: M A
authors.initials: MA
authors.lastname: Mandrand-Berthelot
authors.forename: J C
authors.initials: JC
authors.lastname: Fontecilla-Camps
mesh_terms: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D009532:Nickel / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C089849:nikA protein, E coli; D009532:Nickel