title: A novel fucosylated glycosphingolipid from the millipede, Parafontaria laminata armigera.
abstract: A novel fucosylated glycosphingolipid (GL-3a) was isolated and purified from whole tissues of the millipede, Parafontaria laminata armigera. Its chemical structure was characterized as Man beta 1-4(Fuc alpha 1-3)Glc beta 1-ceramide (I3 alpha Fuc,MlOse2Cer) by gas-liquid chromatography, permethylation study, partial acid hydrolysis, exoglycosidase degradation, TLC/enzyme-immunostaining, negative fast atom bombardment-mass spectrometry and proton nuclear magnetic resonance spectroscopy. This compound was unique in containing a fucose branch linked to the glucose residue of the disaccharide, mannosylglucose. The ceramide moiety was mainly composed of d17:1 (64.3%) and d18:1 (20.0%) sphingoids, and 22:0 (41.8%), 23:0 (16.4%) and 24:0 (15.8%) fatty acids.
authors.affiliation: Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sugita
authors.forename: C
authors.initials: C
authors.lastname: Hayata
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Takeda
authors.forename: T
authors.initials: T
authors.lastname: Hori
authors.forename: F
authors.initials: F
authors.lastname: Nakatani
mesh_terms: D000818:Animals; D001181:Arthropods / Q000737:chemistry*; D002240:Carbohydrate Sequence; D005643:Fucose / Q000737:chemistry*; D006028:Glycosphingolipids / Q000737:chemistry / Q000302:isolation & purification*; D008745:Methylation; D008969:Molecular Sequence Data; D014281:Trihexosylceramides / Q000737:chemistry
chemical_list: D006028:Glycosphingolipids; D014281:Trihexosylceramides; C090437:mannosyl-1-4-(fucosyl-1-3)glucosyl-1-ceramide; D005643:Fucose