6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification.
Crowe
J
J
QIAGEN Inc., Chatsworth, CA.
Döbeli
H
H
Gentz
R
R
Hochuli
E
E
Stüber
D
D
Henco
K
K
D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006639:Histidine; D009532:Nickel / Q000378:metabolism; D009571:Nitrilotriacetic Acid; D009875:Operator Regions, Genetic; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification*
D002614:Chelating Agents; D011993:Recombinant Fusion Proteins; D006639:Histidine; D009532:Nickel; D009571:Nitrilotriacetic Acid