Human erythrocyte membrane protein 4.2 is palmitoylated.
Protein 4.2 is a major protein of the human erythrocyte membrane. It has previously been shown to be N-myristoylated. After labeling of intact human erythrocytes with [3H]palmitic acid, radioactivity was found to be associated with protein 4.2 by immunoprecipitation of peripheral membrane proteins extracted at pH 11 from ghosts with anti-(4.2) sera, followed by SDS/PAGE and fluorography. The fatty acid linked to protein 4.2 was identified as palmitic acid after hydrolysis of protein and thin-layer chromatography of the fatty acid extracted in the organic phase. Protein 4.2 could be depalmitoylated with hydroxylamine, suggesting a thioester linkage. Depalmitoylated protein 4.2 showed significantly decreased binding to protein-4.2-depleted membranes, compared to native protein 4.2.
Das
A K
AK
Department of Chemistry, Bose Institute, Calcutta, India.
Bhattacharya
R
R
Kundu
M
M
Chakrabarti
P
P
Basu
J
J
D001798:Blood Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D015153:Blotting, Western; D003598:Cytoskeletal Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019811:Hydroxylamine; D006898:Hydroxylamines; D007457:Iodine Radioisotopes; D007700:Kinetics; D008565:Membrane Proteins / Q000097:blood / Q000737:chemistry*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000032:analysis* / Q000097:blood; D014316:Tritium
D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D006898:Hydroxylamines; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D010169:Palmitic Acids; C044791:erythrocyte membrane band 4.2 protein; D014316:Tritium; D019811:Hydroxylamine; D019308:Palmitic Acid