title: Toxin production, adherence and protein expression by clinical Aeromonas spp. isolates in broth and human pooled ileostomy fluid.
abstract: The physiological behaviour of clinical Aeromonas spp. isolates was compared following culture in a conventional broth and human pooled ileostomy fluid (PIF). Protein expression was markedly affected by the growth medium, with an overall reduction in whole cell proteins in bacteria grown in ileostomy fluid. In addition, novel outer membrane proteins were produced in PIF but not in broth. The majority of A. hydrophila and A. sobria isolates produced toxin in both broth and PIF, whereas no cytotoxin positive A. caviae were found. Toxin titres were at least two doubling dilutions higher in 40% and 21% of A. hydrophila and A. sobria isolates, respectively, following culture in brain heart infusion broth compared with PIF. Bacterial adherence to Vero and A-549 cells was significantly more common in A. hydrophila (53%) and A. sobria (64%) than in A. caviae (15%) (P < 0.01). We observed increased adherence by 6 aeromonas strains previously classified as adherence-positive, but not by 6 non-adherers, in PIF compared with brain heart infusion broth. The influence of growth medium on the expression of potential virulence determinants by Aeromonas spp. provides a rationale for the use of human ileostomy fluid in future in vitro studies, in order to simulate the nutrient conditions found in vivo.
authors.affiliation: Department of Experimental and Clinical Microbiology, University of Sheffield Medical School.
authors.forename: M H
authors.initials: MH
authors.lastname: Wilcox
authors.forename: A
authors.initials: A
authors.lastname: Cook
authors.forename: I
authors.initials: I
authors.lastname: Geary
authors.forename: A
authors.initials: A
authors.lastname: Eley
mesh_terms: D000333:Aeromonas / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001826:Body Fluids / Q000382:microbiology; D002522:Chlorocebus aethiops; D003470:Culture Media / Q000378:metabolism; D003603:Cytotoxins / Q000096:biosynthesis*; D006801:Humans; D007081:Ileostomy; D013045:Species Specificity; D014407:Tumor Cells, Cultured; D014709:Vero Cells
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D003470:Culture Media; D003603:Cytotoxins