Endothelins activate phospholipase A2 in brain capillary endothelial cells.
Addition of endothelin-1 to cultured rat brain capillary endothelial cells induced a 2.7-fold activation of phospholipase A2, as evidenced from the release of [3H]arachidonic acid from prelabelled cells. Half maximum activation by endothelin-1 was observed at 1 nM. The action of endothelin-1 was not mimicked by low concentrations of endothelin-3 and it was largely suppressed by BQ-123, suggesting the involvement of an ETA receptor subtype. It is suggested that the activation of phospholipase A2 by endothelins plays a role in the development of delayed cerebral vasospasm following subarachnoid hemorrhage.
Vigne
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Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, Université de Nice-Sophia Antipolis, Valbonne, France.
Frelin
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D000818:Animals; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000187:drug effects / Q000201:enzymology; D065128:Endothelin Receptor Antagonists; D016232:Endothelins / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000201:enzymology*; D002546:Ischemic Attack, Transient / Q000378:metabolism; D010456:Peptides, Cyclic / Q000494:pharmacology; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D013345:Subarachnoid Hemorrhage / Q000378:metabolism
D065128:Endothelin Receptor Antagonists; D016232:Endothelins; D010456:Peptides, Cyclic; D010741:Phospholipases A; D054467:Phospholipases A2; C072247:cyclo(Trp-Asp-Pro-Val-Leu)