title: Evaluation of preanalytical variables associated with measurement of prothrombin fragment 1.2.
abstract: We have used a monoclonal antibody-based ELISA for plasma prothrombin fragment 1.2 (F1.2) to establish appropriate sample collection and storage conditions for this biomarker of thrombin generation. F1.2 concentrations were not altered by exogenous factor Xa, thrombin, or thromboplastin if blood was collected by routine venipuncture into tubes containing heparin as anticoagulant (but not citrate, acid-citrate-dextrose, EDTA, or oxalate) and if plasma antithrombin III concentration was > or = 30% of normal. Heparinized plasma F1.2 was stable for > or = 8 h at 20-25 degrees C, and if premixed with a stabilizing reagent, for > or = 4 years at -70 degrees C. Mean values for heparinized plasma F1.2 collected and stored by recommended procedures were increased in patients with thrombosis and conditions of increased thrombotic risk, and were sensitive to heparin and oral anticoagulant therapies. We conclude that plasma obtained by routine venipuncture into tubes with heparin as anticoagulant is an appropriate specimen for F1.2 measurements for most patients.
authors.affiliation: Division of Hematology/Oncology, Duke University Medical Center, Durham, NC 27710.
authors.forename: C S
authors.initials: CS
authors.lastname: Greenberg
authors.forename: M J
authors.initials: MJ
authors.lastname: Hursting
authors.forename: B G
authors.initials: BG
authors.lastname: Macik
authors.forename: T L
authors.initials: TL
authors.lastname: Ortel
authors.forename: W H
authors.initials: WH
authors.lastname: Kane
authors.forename: B M
authors.initials: BM
authors.lastname: Moore
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D000925:Anticoagulants / Q000627:therapeutic use; D000990:Antithrombin III / Q000378:metabolism; D020152:Antithrombin III Deficiency; D001800:Blood Specimen Collection / Q000379:methods; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D006493:Heparin; D006801:Humans; D008875:Middle Aged; D010446:Peptide Fragments / Q000032:analysis* / Q000378:metabolism; D010974:Platelet Aggregation; D010976:Platelet Count; D011516:Prothrombin / Q000032:analysis* / Q000378:metabolism; D012016:Reference Values; D013927:Thrombosis / Q000097:blood
chemical_list: D000911:Antibodies, Monoclonal; D000925:Anticoagulants; D010446:Peptide Fragments; C045879:prothrombin fragment 1.2; D000990:Antithrombin III; D011516:Prothrombin; D006493:Heparin