Epidermal growth factor induces protein tyrosine phosphorylation and association of p190 with ras-GTP-ase activating protein in Caco-2 cells.
Epidermal growth factor (EGF) modulates several functions of human enterocytes. We report that this growth factor induces strong tyrosine phosphorylation stimulation of its receptor and several putative substrates of the receptor intrinsic kinase including c-erb B2 in proliferating human colon carcinoma cells (Caco-2). In addition EGF induces stable association of the GTP-ase activating protein of p21ras to the p190 protein and to a 62 mol.wt. tyrosine-phosphorylated protein. This association is probably consequent to EGF stimulation of protein tyrosine phosphorylation and could coordinate progression through cell cycle with polarity, cell-cell interactions and cell mobility.
Auricchio
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Dipartimento di Pediatria, Facoltà di Medicina e Chirurgia, Università di Napoli Federico II, Italia.
di Domenico
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Castoria
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Bilancio
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Migliaccio
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D000906:Antibodies; D015153:Blotting, Western; D004815:Epidermal Growth Factor / Q000276:immunology / Q000502:physiology*; D020690:GTPase-Activating Proteins; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D012097:Repressor Proteins; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000276:immunology / Q000378:metabolism*; D020703:ras GTPase-Activating Proteins; D020666:ras-GRF1
D000906:Antibodies; D004815:Epidermal Growth Factor; D020690:GTPase-Activating Proteins; D020662:Guanine Nucleotide Exchange Factors; D009687:Nuclear Proteins; D010750:Phosphoproteins; D011506:Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018719:Erb-b2 Receptor Tyrosine Kinases; D012097:Repressor Proteins; D014443:Tyrosine; D020703:ras GTPase-Activating Proteins; D020666:ras-GRF1; C074560:ARHGAP35 protein, human; C513039:RASGRF1 protein, human; C501009:HRAS protein, human