Chromatographic analysis of selected tetracyclines from dosage forms and bulk drug substance using polymeric columns with acidic mobile phases.
The LC analysis of selected tetracyclines from dosage forms and bulk drug substance using polymeric columns has been studied. Mobile phases containing acetonitrile-0.02 M sodium perchlorate (pH 2.0) were used. The tetracyclines were detected by their absorbance at 280 nm. The columns included: a polystyrene-divinylbenzene (PS-DVB) column and a polymethacrylate column with octadecyl ligands (PM-C18). Performance of the two columns was compared and applicability of the described methods for compendial use has been evaluated. The tetracyclines investigated include: minocycline, oxytetracycline, tetracycline, demeclocycline, chlortetracycline, methacycline, doxycycline and meclocycline.
Bryan
P D
PD
Department of Medicinal Chemistry, College of Pharmacy, University of Georgia, Athens 30602-2352.
Stewart
J T
JT
D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004304:Dosage Forms; D006863:Hydrogen-Ion Concentration; D010472:Perchlorates / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D012015:Reference Standards; D012117:Resins, Synthetic; D017670:Sodium Compounds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013754:Tetracyclines / Q000032:analysis*
D000097:Acetonitriles; D004304:Dosage Forms; D010472:Perchlorates; D011109:Polymethacrylic Acids; D011137:Polystyrenes; D012117:Resins, Synthetic; D017670:Sodium Compounds; D013754:Tetracyclines; C030613:polymethacrylic acid; C003771:Amberlite XAD-2 resin; C031068:sodium perchlorate; C032159:acetonitrile