Acquired thermotolerance and heat shock proteins in thermophiles from the three phylogenetic domains.
Thermophilic organisms from each of the three phylogenetic domains (Bacteria, Archaea, and Eucarya) acquired thermotolerance after heat shock. Bacillus caldolyticus grown at 60 degrees C and heat shocked at 69 degrees C for 10 min showed thermotolerance at 74 degrees C, Sulfolobus shibatae grown at 70 degrees C and heat shocked at 88 degrees C for 60 min showed thermotolerance at 95 degrees C, and Thermomyces lanuginosus grown at 50 degrees C and heat shocked at 55 degrees C for 60 min showed thermotolerance at 58 degrees C. Determinations of protein synthesis during heat shock revealed differences in the dominant heat shock proteins for each species. For B. caldolyticus, a 70-kDa protein dominated while for S. shibatae, a 55-kDa protein dominated and for T. lanuginosus, 31- to 33-kDa proteins dominated. Reagents that disrupted normal protein synthesis during heat shock prevented the enhanced thermotolerance.
Trent
J D
JD
Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, Illinois 60439.
Gabrielsen
M
M
Jensen
B
B
Neuhard
J
J
Olsen
J
J
D000222:Adaptation, Physiological; D001407:Bacillus / Q000502:physiology*; D003513:Cycloheximide / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D003904:Mitosporic Fungi / Q000502:physiology*; D014176:Protein Biosynthesis / Q000187:drug effects; D016944:Sulfolobus / Q000502:physiology*
D006360:Heat-Shock Proteins; D003513:Cycloheximide