Proliferation of human bone marrow cells in vitro: inhibition by Candida albicans components.
We studied the influence of human recombinant granulocyte-macrophage colony-stimulating factor (rhGM-CSF) and Candida albicans (CA) components, either alone or in combination, on the proliferation of human bone marrow cells in vitro (colony-forming assay). The number of colonies per 10(5) bone marrow cells after cultivation with rhGM-CSF [maximal (plateau) colony formation] was: 46.2 +/- 9.1 (n = 6); after cultivation with rhGM-CSF in combination with CA components the numbers of colonies were as follows: 0.05 mg protein/ml, 33.4 +/- 4.6 (n = 3); 0.10 mg protein/ml, 20.8 +/- 3.6 (n = 3). The mechanisms responsible for this inhibition of colony formation are still unknown. They may be of pathogenetic significance in CA infection.
Diezel
W
W
Department of Dermatology, University of Rostock Medical School, Germany.
Greiner
J
J
Geib
A
A
Kaben
U
U
D001853:Bone Marrow / Q000187:drug effects / Q000382:microbiology; D001854:Bone Marrow Cells; D002176:Candida albicans / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor