Lack of germ-line mutations in the p53 gene exons 4 to 8 in patients with late-onset second malignant neoplasms.
Previous studies have shown p53 germ-line mutations in some familial cancer aggregations with or without the Li-Fraumeni syndrome (LFS). Such mutations were also reported in children and young adults with second malignant neoplasms (SMN). This led us to screen for p53 germ-line mutations in a group of seven patients affected with SMN, but characterized by an older age of onset than in the previous reports. No mutation was found in exons 4 to 8 and their boundaries using the single-strand conformation polymorphism technique. Our results give strong evidence for genetic heterogeneity of SMN, probably related to the age of cancer onset.
Lalle
P
P
Molecular Oncology Laboratory, Centre Jean Perrin, Clermont-Ferrand, France.
Moyret
C
C
Bignon
Y J
YJ
D000328:Adult; D000368:Aged; D001483:Base Sequence; D005091:Exons; D016158:Genes, p53 / Q000235:genetics*; D018095:Germ-Line Mutation / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D016609:Neoplasms, Second Primary / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013997:Time Factors