PCR-RFLP studies on chromosome 3p in formaldehyde-fixed, paraffin-embedded cervical cancer tissues.
Loss of heterozygosity (LOH) has been extensively studied on the short arm of chromosome 3, and functional proofs have been obtained defining a tumor-suppressor locus at 3p21-22. We examined 31 paraffin-embedded cervical cancer samples for LOH, using 5 PCR-primer pairs, located around 3p21. Allele loss was found in 19 out of the 27 informative samples (70%) while 13 out of 23 informative samples (56%) had LOH located at 3p21-22. More of the human papillomavirus (HPV)-positive samples had LOH compared to the HPV-negative samples, giving only a weak association between loss of allele and HPV integration. Modifications of the DNA in the formaldehyde-fixed samples were detected, and further studies will be required to clarify how such artifacts may affect restriction fragment length polymorphism (RFLP) studies on fixed tissues.
Karlsen
F
F
Department of Pathology, Norwegian Radium Hospital, Montebello, Oslo.
Rabbitts
P H
PH
Sundresan
V
V
Hagmar
B
B
D000483:Alleles; D001483:Base Sequence; D002893:Chromosomes, Human, Pair 3; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics*; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology*; D017353:Gene Deletion; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D016707:Tissue Fixation; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
D004273:DNA, Neoplasm; D005557:Formaldehyde