Histamine-induced microvascular leakage in pial venules: differences between the SJL/J and BALB/c inbred strains of mice.
The actions of histamine on pial venule leaky site formation were measured intravitally in two inbred strains of mice (BALB/c and SJL/J). Pial venules were visualized using a cranial window microscopy technique, and microvascular leaky site formation was assessed visually using a fluorescein-dextran indicator. SJL/J mice were found to be sensitive to histamine-induced leakage, whereas the BALB/c strain was refractory. Exposure to pertussis toxin enhanced the sensitivity to histamine in the SJL/J strain, but little effect was observed for BALB/c mice. However, the employment of a polymerase chain reaction (PCR) technique for the detection of mRNA for histamine H1 receptor identified receptor-specific message in isolated cerebrovascular endothelium from both strains of mice. The lack of pial responsiveness in the BALB/c mice remains unexplained. Mast cells in the dura mater were found to be more numerous in SJL/J mice than in BALB/c mice. This observation supports previous observations of strain-specific differences in CNS inflammation. The results support the concept that genetically controlled differences in vascular sensitivity and localization of CNS-associated mast cells may play important roles in the generation of vasogenic edema and inflammation in CNS trauma and disease.
Yong
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Department of Medical Physiology, Texas A&M University, College Station.
Bebo
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Sapatino
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Welsh
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Orr
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Linthicum
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D000818:Animals; D001160:Arterioles / Q000033:anatomy & histology / Q000187:drug effects; D001483:Base Sequence; D001812:Blood-Brain Barrier / Q000187:drug effects*; D002550:Cerebral Veins / Q000187:drug effects / Q000502:physiology*; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D037342:Pertussis Toxin; D012333:RNA, Messenger / Q000096:biosynthesis; D011969:Receptors, Histamine H1 / Q000096:biosynthesis; D013045:Species Specificity; D014699:Venules / Q000033:anatomy & histology / Q000187:drug effects; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D006634:Histamine H1 Antagonists; D012333:RNA, Messenger; D011969:Receptors, Histamine H1; D010566:Virulence Factors, Bordetella; D006632:Histamine; D037342:Pertussis Toxin