title: Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase.
abstract: The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycosylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety was analysed by fast-atom bombardment mass spectrometry and gas chromatography-mass spectrometry. Taken together the data suggest the structure, Thr-Asp-ethanolamine-PO4-Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN-(sn-1-O- alkyl-2-O-acylglycerol-3-PO4-1-myo-D-inositol), which contains an additional ethanolamine phosphate group at an unknown position.
authors.affiliation: Department of Biochemistry, University of Dundee, U.K.
authors.forename: C A
authors.initials: CA
authors.lastname: Redman
authors.forename: J E
authors.initials: JE
authors.lastname: Thomas-Oates
authors.forename: S
authors.initials: S
authors.lastname: Ogata
authors.forename: Y
authors.initials: Y
authors.lastname: Ikehara
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D002240:Carbohydrate Sequence; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D017261:Glycosylphosphatidylinositols / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D017261:Glycosylphosphatidylinositols; D000469:Alkaline Phosphatase