title: Dual pathways regulate neurite outgrowth in enteric ganglia.
abstract: Primary cultures of guinea pig myenteric plexus ganglia were used to examine the ability of agents that activate adenylate cyclase or mimic intracellular adenosine 3',5'-cyclic monophosphate (cAMP) to stimulate morphological growth. Dose-dependent increases in neurite length and density were produced in enteric neuronal cultures by forskolin (212% of control), cholera toxin (356% of control), or the permeant cAMP analogues 8-bromoadenosine 3',5'-cyclic monophosphate and dibutyryl cAMP. (R)-p-adenosine 3',5'-cyclic monophosphorothioate, an inhibitor of cAMP-dependent kinases, blocked the growth-promoting effects of cAMP analogues but not of nerve growth factor (NGF). Activation of cAMP-dependent signaling pathways also increased production of mRNA for alpha-tubulin and microtubule-associated protein 2. Dual pathways, regulated by NGF and cAMP-dependent protein kinases, influence growth signaling in enteric ganglia.
authors.affiliation: Department of Surgery, University of Michigan, Ann Arbor 48109-0331.
authors.forename: D M
authors.initials: DM
authors.lastname: Simeone
authors.forename: G
authors.initials: G
authors.lastname: Romanchuk
authors.forename: M W
authors.initials: MW
authors.lastname: Mulholland
mesh_terms: D000818:Animals; D001483:Base Sequence; D000242:Cyclic AMP / Q000378:metabolism; D005724:Ganglia / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006168:Guinea Pigs; D007422:Intestines / Q000294:innervation*; D007425:Intracellular Membranes / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism; D016501:Neurites / Q000502:physiology*; D015345:Oligonucleotide Probes / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D013268:Stimulation, Chemical; D014404:Tubulin / Q000235:genetics
chemical_list: D008869:Microtubule-Associated Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014404:Tubulin; D000242:Cyclic AMP