Identification of fenestrated capillary segments in microvascular corrosion casts of the rat exocrine pancreas.
The capillary bed of the rat exocrine pancreas was studied by scanning and transmission electron microscopy of corrosion casts and tissue sections. Two types of capillaries were distinguished in corrosion casts. First, there were straight capillaries of relatively constant width (mean diameter 4.79 +/- 0.87 microns), which were characterized by numerous circular constrictions on their surface. About 37% of the capillaries belonged to this type. Second, there were undulating capillaries which showed smooth surfaced eccentric dilatations defined by similar surface constrictions. The bulging areas measured 8.43 +/- 1.33 microns, the constrictions next to the bulges figured for 6.45 +/- 1.53 microns. About 63% of the capillaries belonged to the second type. Two types of capillaries were also identified in tissue sections. First, there were capillaries with continuous endothelial lining (26% of capillary profiles; mean diameter 5.48 +/- 1.67 microns); 27% of their endothelial lining was provided with underlying pericytes. Second, there were capillaries with fenestrated endothelium (64% of capillary profiles; mean diameter 6.16 +/- 1.75 microns); 12% of their endothelial lining was accompanied by pericytes. According to frequency and dimension of these two types of capillaries, we conclude that bulged and undulating capillary casts correspond to fenestrated capillaries and straight capillary casts of constant width correspond to nonfenestrated capillaries. The frequency of crests on the surface of capillary casts correlates with the different frequency of pericyte processes on fenestrated and nonfenestrated capillaries. It is concluded that pericyte processes beneath the endothelium hold resistance against luminal pressure. Bulging areas of capillary casts correlate with fenestrated areas of endothelial lining, that is, areas which are not reinforced by pericyte processes.
Aharinejad
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First Department of Anatomy, University of Vienna, Austria.
Böck
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D000818:Animals; D002196:Capillaries / Q000648:ultrastructure*; D016319:Corrosion Casting; D004730:Endothelium, Vascular / Q000648:ultrastructure; D005260:Female; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010179:Pancreas / Q000098:blood supply* / Q000000981:diagnostic imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D014463:Ultrasonography