Comparison of soybean and pea ENOD40 cDNA clones representing genes expressed during both early and late stages of nodule development.
A pea cDNA clone representing the homologue of the soybean pGmENOD40-1 was isolated and characterized. At the nucleotide level both clones share 55% homology. Strikingly, the homology between the polypeptides derived from the pea and soybean ENOD40 cDNA sequences is only 14%. Despite this low homology Southern analyses revealed that the isolated pea cDNA clone represents the single pea ENOD40. In situ hybridizations showed that at early stages of nodule development and in mature nodules the expression pattern of pea ENOD40 is comparable to that of soybean ENOD40. Although ENOD40 show similar expression patterns in these two nodules, it is questionable whether the putative polypeptides have a similar function, since the homology is very low.
Matvienko
M
M
Department of Molecular Biology, Agricultural University, Wageningen, Netherlands.
Van de Sande
K
K
Yang
W C
WC
van Kammen
A
A
Bisseling
T
T
Franssen
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D018532:Pisum sativum / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology; D062085:RNA, Long Noncoding; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D022661:RNA, Untranslated / Q000502:physiology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013025:Glycine max / Q000235:genetics*
D018076:DNA, Complementary; C405430:ENOD40 RNA; D010940:Plant Proteins; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D018749:RNA, Plant; D022661:RNA, Untranslated