Effects of alpha-interferon and steroids on CD23 expression and release in B-cell chronic lymphocytic leukemia.
BACKGROUND
Since high CD23 expression and release have been reported in B-chronic lymphocytic leukemia (B-CLL), we investigated whether alpha-interferon or corticosteroids were able to modulate the expression and/or the release of this factor.


METHODS
CD23 expression was determined with FITC-labelled anti-CD23 monoclonal antibody, and sCD23 release with a sandwich enzyme immunoassay. Twenty-one patients affected by B-CLL (stage A or B) were studied before and after three different treatment regimens (alpha-interferon, corticosteroids, alpha-interferon+corticosteroids).


RESULTS
CD23 was highly expressed in the B-cells of all patients, and expression was not modified by any of the therapies, sCD23 release from leukemic cells was significantly greater (p < 0.00001) in untreated subjects than controls, and in vitro treatment with phorbol myristate acetate (PMA) led to a 10-fold increase (p < 0.0001) in sCD23 secretion. On the contrary, PMA did not increase sCD23 release in normal B cells. Treatment with corticosteroids (either alone or associated with alpha-interferon) reduced sCD23 secretion from leukemic cells, whereas alpha-interferon alone was not able to modify sCD23 release.


CONCLUSIONS
Our data support the hypothesis that CD23 plays a role in the maintenance and progression of B-CLL and that the pharmacological modulation of this receptor/lymphokine could be useful in the therapy of B-CLL.
Pozzato
G
G
Istituto di Patologia Medica, Cattedra di Medicina Interna II, Università di Trieste, Ospedale di Pordenone, Italy.
de Paoli
P
P
Franzin
F
F
Tulissi
P
P
Moretti
M
M
Basaglia
G
G
Santini
G F
GF
D000368:Aged; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000188:drug therapy* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D017455:Receptors, IgE / Q000096:biosynthesis / Q000187:drug effects* / Q000378:metabolism; D013256:Steroids / Q000494:pharmacology*
D016898:Interferon-alpha; D017455:Receptors, IgE; D013256:Steroids