Characterization of a Borna disease virus glycoprotein, gp18.
Borna disease virus is a nonsegmented negative-strand RNA virus that causes neurologic disease in a wide variety of animal hosts. Here we describe identification and characterization of the first glycoprotein in this viral system. The 18-kDa glycoprotein, gp18, has been purified from infected rat brain. Isolation and microsequencing of this protein allowed identification of a 16.2-kDa open reading frame in the viral antigenome. Lectin binding and endoglycosidase sensitivity assays indicate that gp18 is an unusual N-linked glycoprotein.
Kliche
S
S
Department of Neurology, University of California, Irvine 92717.
Briese
T
T
Henschen
A H
AH
Stitz
L
L
Lipkin
W I
WI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001891:Borna disease virus / Q000737:chemistry*; D006023:Glycoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D051381:Rats; D011917:Rats, Inbred Lew; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*
D006023:Glycoproteins; D014764:Viral Proteins; C089953:gp18 protein, Borna disease virus