Molecular cloning of rat bone morphogenetic protein (BMP) type IA receptor and its expression during ectopic bone formation induced by BMP.
A cDNA for the rat bone morphogenetic protein (BMP) type IA receptor (BMPR-IA) was isolated from a dental pulp cell cDNA library. The rat BMPR-IA cDNA encodes a protein of 532 amino acids with a single transmembrane domain and a putative serine/threonine kinase domain. The overall amino acid sequence identity between the rat and human BMPR-IA was 97%. Reverse transcriptase-polymerase chain reaction analysis revealed that BMPR-IA mRNA was highly expressed in the BMP-induced bone forming tissues throughout the stages tested.
Takeda
K
K
Department of Biochemistry, Tokyo Medical and Dental University, Japan.
Oida
S
S
Ichijo
H
H
Iimura
T
T
Maruoka
Y
Y
Amagasa
T
T
Sasaki
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001846:Bone Development / Q000187:drug effects; D052004:Bone Morphogenetic Protein Receptors; D052005:Bone Morphogenetic Protein Receptors, Type I; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones; D002828:Choristoma / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000302:isolation & purification; D003782:Dental Pulp / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D015723:Gene Library; D006133:Growth Substances / Q000096:biosynthesis*; D006801:Humans; D008969:Molecular Sequence Data; D009135:Muscular Diseases / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D017978:Receptors, Growth Factor; D017386:Sequence Homology, Amino Acid
D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D006133:Growth Substances; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; C498213:Bmpr1a protein, rat; D052004:Bone Morphogenetic Protein Receptors; D052005:Bone Morphogenetic Protein Receptors, Type I