Random amplified polymorphic DNA genotyping of Mycobacterium malmoense.
The suitability of random amplified polymorphic DNA-PCR for the detection of differences between Mycobacterium malmoense strains was evaluated. With two of the 32 tested primers seven fingerprint patterns which proved excellent in distinguishing intraspecies variations of M. malmoense were obtained. The combination of the results obtained with the two primers permitted a clear separation of the strains. This technique is useful for analyzing species whose DNA sequences are not known. It can easily be adapted to test any mycobacterial species.
Kauppinen
J
J
Department of Clinical Microbiology, University of Kuopio, Finland.
Mäntyjärvi
R
R
Katila
M L
ML
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
D004269:DNA, Bacterial