title: Matrix-assisted laser desorption/ionization mass spectrometry of restriction enzyme-digested plasmid DNA using an active Nafion substrate.
abstract: Matrix-assisted laser desorption/ionization using a 3-hydroxypicolinic acid matrix from an active Nafion substrate has been used for detection of restriction enzyme-digested double-stranded plasmid DNA using time-of-flight mass spectrometry. DNA strands of up to 267 base pairs were detected with minimal sample purification, although only as species corresponding to single-stranded DNA.
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor 48109.
authors.forename: J
authors.initials: J
authors.lastname: Bai
authors.forename: Y H
authors.initials: YH
authors.lastname: Liu
authors.forename: D M
authors.initials: DM
authors.lastname: Lubman
authors.forename: D
authors.initials: D
authors.lastname: Siemieniak
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000737:chemistry; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D006801:Humans; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010848:Picolinic Acids; D010957:Plasmids / Q000737:chemistry*; D016133:Polymerase Chain Reaction
chemical_list: D005465:Fluorocarbon Polymers; D010848:Picolinic Acids; C040402:perfluorosulfonic acid; C049246:3-hydroxypicolinic acid; D004247:DNA; D004262:DNA Restriction Enzymes