title: Lipopolysaccharides of Legionella erythra and Legionella oakridgensis.
abstract: The chemical composition of lipopolysaccharides from Legionella erythra and Legionella oakridgensis was analysed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed both lipopolysaccharides to have a smooth-type character. The polysaccharide part of both lipopolysaccharides contained D-mannose, D-glucose, D-glycero-D-mannoheptose, L-glycero-D-manno-heptose, 2-keto-3-deoxyoctonic acid, L-fucosamine, D-glucosamine, and glucosamine phosphate. In addition, L-rhamnose, glycerol phosphate, and glucose phosphate were identified in the polysaccharide part of L. erythra lipopolysaccharide. The main sugar identified in the lipid A part of both lipopolysaccharides, 2,3-diamino-2,3-dideoxy-D-glucose, was found to be substituted with a complex fatty acid composition including at least 16 different amide-linked 3-hydroxy fatty acids. Both lipopolysaccharides contained nonhydroxy fatty acids and the uncommon 27-oxo-octacosanoic acid, 29-oxotriacontanoic acid, and 27-hydroxyoctacosanoic acid. The lipopolysaccharide of L. oakridgensis also contained 29-hydroxytriacontanoic acid. The dioic long-chain acids heptacosane-1,27-dioic acid and nonacosane-1,29-dioic acid were only present in the lipopolysaccharide of L. erythra.
authors.affiliation: National Institute of Public Health, Oslo, Norway.
authors.forename: A
authors.initials: A
authors.lastname: Sonesson
authors.forename: E
authors.initials: E
authors.lastname: Jantzen
authors.forename: T
authors.initials: T
authors.lastname: Tangen
authors.forename: U
authors.initials: U
authors.lastname: Zähringer
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D005944:Glucosamine / Q000031:analogs & derivatives / Q000032:analysis; D007875:Legionella / Q000737:chemistry* / Q000145:classification; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: D002241:Carbohydrates; D005227:Fatty Acids; D008070:Lipopolysaccharides; C040138:2,3-diamino-2,3-dideoxyglucose; D005944:Glucosamine