[Quantitative determination of dose-response relations of taxol and cisplatin in ovarian cancer cells with a bioassay in comparison with cytomorphology].
The dose-response relations of the cytotoxic drugs taxol and cisplatin and their combination were analyzed in an ovarian cancer cell line (BG-1). The chemosensitivity profiles were obtained with 5 concentrations using the ATP cell viability assay (ATP-CVA). Furthermore, the cytomorphologic changes were analyzed. Our findings demonstrate that the ATP-CVA is able to determine the fraction of surviving cells and has proved to be an effective tool to measure cytotoxicity. At the same time we were able to analyze the cytomorphologic changes. The implications of these results for the practice of gynecologic oncology are discussed.
Köchli
O R
OR
Departement für Frauenheilkunde, Universitätsklinik Zürich, Schweiz.
Landolt
U
U
Schär
G
G
Sevin
B U
BU
Haller
U
U
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology
D017239:Paclitaxel; D002945:Cisplatin
Quantitative Erfassung der Dosis-Wirkungs-Beziehungen der Zytostatika Taxol und Cisplatin an Ovarialkarzinomzellen mit einem Bioassay im Vergleich zur Zytomorphologie.