The reductive acetyl coenzyme A pathway: sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum.
The methyltransferase (MeTr) from Clostridium thermoaceticum transfers the N5-methyl group of (6S)-methyltetrahydrofolate to the cobalt center of a corrinoid/iron-sulfur protein in the acetyl coenzyme A pathway. MeTr was purified to homogeneity and shown to lack metals. The acsE gene encoding MeTr was sequenced and actively expressed in Escherichia coli at a level of 9% of cell protein. Regions in the sequence of MeTr and the E. coli cobalamin-dependent methionine synthase were found to share significant homology, suggesting that they may represent tetrahydrofolate-binding domains.
Roberts
D L
DL
Department of Biochemistry, University of Nebraska, Lincoln 68583-0718.
Zhao
S
S
Doukov
T
T
Ragsdale
S W
SW
D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000235:genetics; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D008670:Metals / Q000032:analysis; D008780:Methyltransferases / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013763:Tetrahydrofolates / Q000378:metabolism*
D001426:Bacterial Proteins; C080269:C-Fe-SP protein, Moorella thermoacetica; D007506:Iron-Sulfur Proteins; D008670:Metals; D013763:Tetrahydrofolates; D000105:Acetyl Coenzyme A; D008780:Methyltransferases; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; C005984:5-methyltetrahydrofolate