title: Lipid peroxidation and antioxidative vitamins under extreme endurance stress.
abstract: The aim of this study was to examine whether extreme endurance stress of trained athletes can influence lipid peroxidation and muscle enzymes. A randomized and placebo-controlled study was carried out on 24 trained long-distance runners who were substituted with alpha-tocopherol (400 I.U. d-1) and ascorbic acid (200 mg d-1) during 4.5 weeks prior to a marathon race. The serum concentrations of retinol, ascorbic acid, beta-carotene, alpha-tocopherol, malondialdehyde (TBARS) and uric acid as well as glutathione peroxidase (GSH Px) and catalase were measured 4.5 weeks before (A), immediately before (B), immediately after (C) and 24 h after (D) the course. After competition (C) TBARS serum concentrations of the athletes (n = 22) decreased in both groups (P < 0.0001). The ascorbic acid serum concentration increased significantly in the supplemented group from (A) to (B) (P < 0.01), from (B) to (C) (P < 0.001) and in the placebo group a significant increase from (B) to (C) (P < 0.01) was observed. The alpha-tocopherol serum concentration increased significantly in the supplemented group from (A) to (B) (P < 0.001) and from (B) to (C) (P < 0.05). The enzymes glutathione peroxidase (GSH Px) and catalase measured in erythrocytes as well as the serum selenium levels did not show significant differences at any time. A significant increase of CK concentration was observed from (C) to (D) in the supplemented group (P < 0.01) and in the placebo group (P < 0.001). The increase of CK serum concentration is remarkably lower in the supplemented group compared with the placebo group (P < 0.01). It is concluded that endurance training coupled with antioxidant vitamin supplementation reduces blood CK increase under exercise stress.
authors.affiliation: University Medical Hospital, Department of Sports and Performance Medicine, Albert-Ludwigs Universität, Freiburg, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Rokitzki
authors.forename: E
authors.initials: E
authors.lastname: Logemann
authors.forename: A N
authors.initials: AN
authors.lastname: Sagredos
authors.forename: M
authors.initials: M
authors.lastname: Murphy
authors.forename: W
authors.initials: W
authors.lastname: Wetzel-Roth
authors.forename: J
authors.initials: J
authors.lastname: Keul
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000097:blood / Q000494:pharmacology*; D001810:Blood Volume; D003402:Creatine Kinase / Q000097:blood; D005080:Exercise Test; D006461:Hemolysis; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D010807:Physical Endurance / Q000502:physiology*; D005082:Physical Exertion; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D003402:Creatine Kinase; D001205:Ascorbic Acid