title: Vitamin E deficiency increases serum thromboxane A2, platelet arachidonate and lipid peroxidation in male Sprague-Dawley rats.
abstract: This study was designed to determine whether dietary linoleate and all-rac-alpha-tocopheryl acetate (vitamin E) interact to affect serum thromboxane A2 (TXA2) and prostacyclin (PGI2) status and therefore, thrombogenic potential. 6 groups of 12 weanling male Sprague-Dawley rats were fed semipurified diets containing 11 or 18% of energy from linoleate and 0, 100 or 5000 mg vitamin E/kg diet for 10 weeks. Platelet and serum alpha-tocopherol concentrations increased logarithmically with increasing dietary vitamin E. Serum TXA2, measured as TXB2, platelet arachidonate and thiobarbituric acid reactive substances were significantly greater in the vitamin E deficient groups than in groups receiving vitamin E (p < 0.05). Serum PGI2 levels, determined as 6-keto-PGF1 alpha, were not affected by diets. No interaction was found between dietary linoleate and vitamin E. However, vitamin E supplementation produced significantly less serum TXB2 than did vitamin E deficient diets (p < 0.05). Vitamin E deficiency may be prothrombogenic by increasing platelet arachidonate, lipid peroxidation and serum TXA2 levels while vitamin E supplementation at levels used in this study may decrease such effects.
authors.affiliation: Department of Food Science and Human Nutrition, Ames, Iowa 50011-1120.
authors.forename: H W
authors.initials: HW
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Hendrich
authors.forename: L R
authors.initials: LR
authors.lastname: Cook
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D016718:Arachidonic Acid / Q000097:blood*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D011464:Epoprostenol / Q000097:blood; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000008:administration & dosage; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013927:Thrombosis / Q000097:blood / Q000209:etiology / Q000517:prevention & control; D013928:Thromboxane A2 / Q000097:blood*; D013929:Thromboxane B2 / Q000097:blood; D014810:Vitamin E / Q000008:administration & dosage; D014811:Vitamin E Deficiency / Q000097:blood* / Q000150:complications
chemical_list: D008041:Linoleic Acids; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D016718:Arachidonic Acid; D013929:Thromboxane B2; D013928:Thromboxane A2; D015121:6-Ketoprostaglandin F1 alpha; D019787:Linoleic Acid; D011464:Epoprostenol