Electron microscopic analysis of in vitro replication products of ors 8, a mammalian origin enriched sequence.
Electron microscopy was used to map the initiation site of ors 8 DNA replication in vitro in a system that is capable of initiating and supporting one round of semiconservative replication of cloned mammalian DNA origin-enriched sequences (ors). Using unique restriction sites in ors 8 plasmid DNA, we have mapped the replication bubble within the monkey DNA sequence. In addition to site-specific initiation within the ors, the results also indicate bidirectional replication.
Pearson
C E
CE
McGill Cancer Centre, Department of Medicine, McGill University, Montreal, Quebec, Canada.
Shihab-el-Deen
A
A
Price
G B
GB
Zannis-Hadjopoulos
M
M
D000818:Animals; D004247:DNA / Q000648:ultrastructure; D004261:DNA Replication / Q000235:genetics*; D000882:Haplorhini; D008854:Microscopy, Electron; D010957:Plasmids / Q000648:ultrastructure; D018741:Replication Origin / Q000235:genetics*
D004247:DNA