Vitamin E supplementation at various levels alters cytokine production by thymocytes during retrovirus infection causing murine AIDS.
Female C57BL/6 mice were infected with LP-BM5 retrovirus, causing murine AIDS which is functionally similar to human AIDS. Retrovirus infection targets the thymus producing altered T-cell differentiation via the dysregulation of thymocyte cytokine production. Therefore the effects of dietary vitamin E at various levels were determined on cytokine production by ConA-stimulated thymocytes from uninfected (normal) and retrovirus-infected mice. Dietary supplementation, with a 15-, 150- and 450-fold increase of vitamin E in the diet modulated interleukin-2 (IL) production in both uninfected mice and retrovirus-infected mice. The 150- and 450-fold vitamin E supplementation significantly reduced IL-4 secretion by thymocytes from the uninfected, normal mice. Supplementation at all levels also significantly reduced IL-4 production by thymocytes, which was elevated by the retrovirus infection. Vitamin E significantly reduced IL-6 and interferon-gamma production increased during the progression to murine AIDS. The effects of dietary vitamin E on conA-induced proliferation of thymocytes were consistent with the finding on changes of IL-2 secretion. No effect of dietary vitamin E on thymus weight was observed in both uninfected and retrovirus-infected mice. These data indicate that dietary vitamin E supplementation at extremely high levels can modulate cytokine production by thymocytes. This could affect T-cell differentiation, especially during murine AIDS when cytokine production was partially normalized by vitamin E supplementation.
Wang
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Y
Department of Family and Community Medicine, University of Arizona, Tucson.
Watson
R R
RR
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage* / Q000032:analysis / Q000097:blood
D016207:Cytokines; D007376:Interleukin-2; D015850:Interleukin-6; D014810:Vitamin E; D015847:Interleukin-4; D007371:Interferon-gamma