Functional analysis of peripheral blood lymphocytes from healthy volunteers.
Functional analysis of peripheral blood lymphocytes (PBL) from 46 healthy donors is described. For quantitation of cytotoxic activity a fluorescence-based assay is performed with commercially available human tumor cell lines as targets. For evaluation of natural-killer cell activity, the cell line K562 (NK-cell sensitive) and for T-cell activity Raji cells (NK-cell resistant) are used as targets. Since we are not using autologous cells as targets we are measuring the function of non MHC-restricted cytotoxic T-lymphocytes. The results show a median NK-cell activity of 77.4% (given in % lysis) and a median T-cell activity of 21.4% in healthy individuals at an effector-/target-cell ratio (E/T ratio) of 40:1. For correlating immunological parameters such as functionality of PBL from tumor patients with the progression of tumor growth, normal values are of great importance.
Donnerstag
B
B
Dept. of Biological Chemistry, Johann Wolfgang Goethe University Medical Center, Frankfurt/Main, Germany.
Oltrogge
J B
JB
Henzel
K
K
Rüenauver
A
A
Baum
R P
RP
D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D007376:Interleukin-2