Estimation of the functional reserve of the human liver by urinary D-glucaric acid excretion after vitamin C administration.
The excretion of D-glucaric acid in the urine (uGA) correlates with the total liver content of hepatic cytochrome P-450, the metabolism of which depends on adenosine triphosphate (ATP) being produced by intrahepatic cellular mitochondria. Five cases of compensated liver cirrhosis group with less than 0.4 mg/kg/min of the maximum removal rate of indocyanine green (ICGR max), and 5 cases with normal hepatic function (control group), were monitored for uGA before and after P-450 activation induced by administration of 1 g of vitamin-C. Before vitamin-C administration, no differences in uGA excretion were observed comparing the cirrhosis with the control group. After administration of vitamin-C, the excretion of uGA was significantly lower in the cirrhosis group. The measurement of uGA is considered to represent vitamin-C induced activation by P-450, and is a new method for evaluation of the functional reserve of the liver.
Hanaue
H
H
Department of Surgery, Tokai University Oiso Hospital, Kanagawa, Japan.
Kanno
K
K
Mukai
M
M
Kubo
H
H
Tobita
K
K
Nakasaki
H
H
Tajima
T
T
Mitomi
T
T
Endo
R
R
D000255:Adenosine Triphosphate / Q000378:metabolism; D000368:Aged; D001205:Ascorbic Acid / Q000008:administration & dosage*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D005937:Glucaric Acid / Q000652:urine*; D006801:Humans; D007208:Indocyanine Green / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism; D008103:Liver Cirrhosis / Q000378:metabolism / Q000652:urine; D008111:Liver Function Tests / Q000379:methods*; D008297:Male; D008875:Middle Aged
D000255:Adenosine Triphosphate; D003577:Cytochrome P-450 Enzyme System; D007208:Indocyanine Green; D001205:Ascorbic Acid; D005937:Glucaric Acid