An automated system for animal exposure to nitrogen dioxide.
We have designed an automated system for controlled exposure of rodents to nitrogen dioxide (NO2). The system consists of 1) two stainless-steel exposure chambers (3.96 m3), for control and treated animals, in which temperature, humidity, and air changes are consistent with current guidelines for rodent housing; 2) a cylinder containing 1% NO2 in N2; 3) a detector connected with a personal computer system that monitors the NO2 concentration, and regulates the NO2 influx, using a closed-loop feedback; and 4) a fan system to distribute the gas uniformly within the chambers. In a typical experiment rats were exposed to 15 ppm of NO2, and changes in the differential count of polymorphonuclear leukocytes, macrophages, and lymphocytes in bronchoalveolar lavage fluid were investigated.
Romano
M
M
Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
Pagani
P
P
Ferro
M
M
Erroi
A
A
Moggi
M
M
Barozzi
F
F
Dal Molin
U
U
Alongi
V
V
Zucchi
C
C
Salmona
M
M
D000818:Animals; D001273:Atmosphere Exposure Chambers; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D008168:Lung / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D009585:Nitrogen Dioxide / Q000633:toxicity*; D051381:Rats
D009585:Nitrogen Dioxide