Characterization of convective solvent flow during iontophoresis.
During iontophoresis under neutral pH conditions, there is a net convective flow of volume (electroosmosis) from anode to cathode leading to the enhanced transport of dissolved polar (but uncharged) solutes in the same direction. The objective of this study was to address the following unresolved questions with respect to electroosmotic transport: [1] Whether the efficiency of electroosmotic transport is solute size-dependent and, if so, how severe is this dependence? [2] Is electroosmosis linearly related to current density in the same way that the iontophoretic flux of charged species appears to be? [3] Are positively charged permeants able to influence their own electrotransport across the skin (by modifying the net charge on the membrane and altering, as a result, the permselectivity) and, if so, why and to what extent? Electroosmosis was assessed from the iontophoreically driven fluxes of mannitol, sucrose and lactose across hairless mouse skin in vitro. It was found that:- (a) The electroosmotic transport rate of mannitol is similar to that of the disaccharides, sucrose and lactose, when examined under identical conditions. The dependence of electroosmotic flux upon molecular size requires study of solutes having a wider range of MW than those considered here. (b) Electroosmotic flow from anode-to-cathode increases with applied current density; similarly, convective flow in the opposite direction diminishes with increasing current density. Apparently, there is correlation between the net movement of solvent and the total flux of ions across the skin. (c) The permselectivity of skin can be 'neutralized' by driving, iontophoretically, a cationic, lipophilic peptide (specifically the leutinizing hormone releasing hormone (LHRH) analog, Nafarelin) into the membrane.(ABSTRACT TRUNCATED AT 250 WORDS)
Delgado-Charro
M B
MB
Department of Pharmacy, University of California, San Francisco 94143-0446.
Guy
R H
RH
D000818:Animals; D066298:In Vitro Techniques; D007478:Iontophoresis; D007785:Lactose / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D008970:Molecular Weight; D009995:Osmosis; D012867:Skin / Q000378:metabolism; D012997:Solvents; D013395:Sucrose / Q000378:metabolism
D012997:Solvents; D008353:Mannitol; D013395:Sucrose; D007785:Lactose