title: Internal tracts of telomeric DNA act as silencers in Saccharomyces cerevisiae.
abstract: Telomeric position effect (TPE) refers to the ability of telomeres to repress the transcription of genes in their vicinity. Internal stretches of C1-3A DNA, the sequence found at Saccharomyces telomeres, also repressed transcription of nearby genes. This repression, hereafter called C1-3A-based silencing, was observed at several chromosomal loci, including on a circular chromosome. The magnitude of C1-3A-based silencing was increased by both proximity to a telomere and increased length of the C1-3A tracts. C1-3A-based silencing was affected by many of the same genes and conditions that influence TPE and acted in an orientation-independent manner. Thus, in yeast, an expanded array of a simple repetitive DNA, C1-3A, is sufficient to promote the assembly of a transcriptionally silent chromosomal domain.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
authors.forename: J B
authors.initials: JB
authors.lastname: Stavenhagen
authors.forename: V A
authors.initials: VA
authors.lastname: Zakian
mesh_terms: D004271:DNA, Fungal / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016615:Telomere / Q000235:genetics*; D015534:Trans-Activators; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal; D015534:Trans-Activators; D009964:Orotidine-5'-Phosphate Decarboxylase