title: Crystallization of Ulex europaeus lectin I.
abstract: The lectin I from Ulex europaeus (UEAI) has a strong affinity for the H-type 2 human blood group determinant. Single crystals of UEAI have been grown in the monoclinic crystal system. Initial crystals were obtained after 11 months from a solution of 10 mg/ml protein, 40% 2,4-methylpentanediol and 0.1 N acetate buffer at pH 5.2. The technique of washing and reseeding was used to generate large suitable crystals. The space group is C2 with a = 78.84 A, b = 69.85 A, c = 120.62 A, beta = 108.74 degrees and Z = 4; there is one molecular dimer per asymmetric unit and the solvent content is estimated to be 58%. The crystals diffract to at least 2.8 A d spacings and are stable in the X-ray beam for more than three days.
authors.affiliation: Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Vandonselaar
authors.forename: L T
authors.initials: LT
authors.lastname: Delbaere
mesh_terms: D002240:Carbohydrate Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D037102:Lectins / Q000737:chemistry*; D008969:Molecular Sequence Data; D037121:Plant Lectins
chemical_list: D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins