Identification and localization of the myotonic dystrophy gene product in skeletal and cardiac muscles.
We have raised a polyclonal antibody against a synthetic peptide chosen within the deduced sequence of the myotonic dystrophy gene product. This antibody binds to a protein whose molecular weight is in the range of 50-54 kDa in Western blotting of rat, rabbit and human muscles. Biochemical studies seem to indicate that this protein is a peripheral component of sarcoplasmic reticulum as well as of plasma membrane.
Salvatori
S
S
CNR, University of Padova, Italy.
Biral
D
D
Furlan
S
S
Marin
O
O
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018656:Muscle Fibers, Fast-Twitch / Q000201:enzymology; D018657:Muscle Fibers, Slow-Twitch / Q000201:enzymology; D009132:Muscles / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009223:Myotonic Dystrophy / Q000235:genetics*; D065636:Myotonin-Protein Kinase; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D017346:Protein Serine-Threonine Kinases / Q000032:analysis* / Q000235:genetics; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D011817:Rabbits; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000201:enzymology
D000906:Antibodies; C585517:DMPK protein, human; D010455:Peptides; D011506:Proteins; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases