Effects of the synthetic glucocorticoid triamcinolone acetonide on vasoactive hydrolases of the human placenta in vitro.
Therapy with glucocorticoids during pregnancy is still debated. Previously reported effects of glucocorticoid application in rats resemble certain symptoms of preeclampsia. Therefore, we studied in vitro the effects of the synthetic glucocorticoid triamcinolone acetonide soluble (0.1-10 mM) on placental alpha-glutamyl amino-peptidase, microsomal alanyl aminopeptidase, dipeptidyl peptidase IV, acetylcholinesterase and butyrylcholinesterase in purified trophoblast monolayers and villous explants from first trimester (n = 5) and term placentae (n = 9) using bio- and histochemical methods. In term placentae quantitative histochemistry (microdensitometry) of trophoblast monolayers revealed an increase of alpha-glutamyl aminopeptidase and microsomal alanyl aminopeptidase activity up to 149% and 126% respectively, after treatment with supraphysiological doses. In trophoblast monolayers from first trimester alpha-glutamyl aminopeptidase activity was not affected, whereas microsomal alanyl aminopeptidase activity increased by 25%. Dipeptidyl peptidase IV staining was reduced to 26%. Biochemical measurements of alpha-glutamyl aminopeptidase and microsomal alanyl aminopeptidase activity in homogenates of cultured villi revealed effects similar to those found by microdensitometry in trophoblast monolayers. In contrast, dipeptidyl peptidase IV activity increased in explants of term placentae by 47%. Acetyl- and butyrylcholinesterase activities were reduced in term placental villi by 38% and 40%, respectively. The data indicate that glucocorticoids may affect the activity of hydrolases which are thought to be involved in local placental blood pressure modulation.
Hahn
T
T
Department of Anatomy, Free University of Berlin, Germany.
Graf
R
R
Oney
T
T
Desoye
G
G
D000110:Acetylcholinesterase / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism; D002091:Butyrylcholinesterase / Q000378:metabolism; D018826:CD13 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D005260:Female; D043384:Glutamyl Aminopeptidase; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D007743:Labor, Obstetric; D010920:Placenta / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014222:Triamcinolone Acetonide / Q000494:pharmacology*; D014327:Trophoblasts / Q000201:enzymology
D006867:Hydrolases; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D000626:Aminopeptidases; D018826:CD13 Antigens; D043384:Glutamyl Aminopeptidase; D018819:Dipeptidyl Peptidase 4; D014222:Triamcinolone Acetonide