title: Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates.
abstract: MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode +/- 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.6% with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.
authors.affiliation: Oregon Health Sciences University, Portland.
authors.forename: M A
authors.initials: MA
authors.lastname: Pfaller
authors.forename: Q
authors.initials: Q
authors.lastname: Vu
authors.forename: M
authors.initials: M
authors.lastname: Lancaster
authors.forename: A
authors.initials: A
authors.lastname: Espinel-Ingroff
authors.forename: A
authors.initials: A
authors.lastname: Fothergill
authors.forename: C
authors.initials: C
authors.lastname: Grant
authors.forename: M R
authors.initials: MR
authors.lastname: McGinnis
authors.forename: L
authors.initials: L
authors.lastname: Pasarell
authors.forename: M G
authors.initials: MG
authors.lastname: Rinaldi
authors.forename: L
authors.initials: L
authors.lastname: Steele-Moore
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D003124:Colorimetry / Q000379:methods*; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D015588:Observer Variation; D015203:Reproducibility of Results
chemical_list: D000666:Amphotericin B; D015725:Fluconazole