Stimulus parameters affecting tissue injury during microstimulation in the cochlear nucleus of the cat.
We investigated the effects of continuous microstimulation in the cats' posteroventral cochlear nucleus, using chronically implanted activated iridium microelectrodes. We examined 51 electrode sites (39 pulsed sites, and 12 unpulsed sites). Seven hours of continuous stimulation at 500 Hz often produced tissue injury near the tips of the pulsed microelectrodes. The damage took the form of a region of vacuolated tissue extending 200 microns or more from the site of the electrode tip. Electron microscope studies showed the vacuoles to be severely edematous segments of myelinated axons. The statistical correlation between the amount of damaged tissue and the charge per phase was large and highly significant (P < 0.0001). When the electrodes were pulsed for 7 h at 500 Hz with charge-balanced biphasic pulse pairs, the threshold for the damage was approximately 3 nC/phase. The damage threshold was not appreciably lower than the stimulation protocol was extended to 35 h (7 h/day for 5 days). In contrast, the threshold for exciting neurons near the microelectrode is approximately 1 nC/phase, as determined by the evoked response recorded in the inferior colliculus. There was little correlation between the severity of the tissue damage and the geometric charge density at the surface of the electrodes, between the damage and amplitude of the cathodic phase of the voltage transient induced across the stimulating electrodes by the stimulus current pulses, or between the damage and the stimulus pulse duration.
McCreery
D B
DB
Huntington Medical Research Institutes, Neurological Research Laboratory, Pasadena, CA 91105.
Yuen
T G
TG
Agnew
W F
WF
Bullara
L A
LA
D000818:Animals; D002415:Cats; D003054:Cochlear Implants / Q000009:adverse effects*; D017626:Cochlear Nucleus / Q000293:injuries* / Q000502:physiology* / Q000648:ultrastructure; D003638:Deafness / Q000628:therapy; D004558:Electric Stimulation / Q000009:adverse effects*; D004599:Electric Stimulation Therapy / Q000009:adverse effects; D004567:Electrodes, Implanted / Q000009:adverse effects; D004594:Electrophysiology; D005260:Female; D006801:Humans; D008839:Microelectrodes / Q000009:adverse effects; D008854:Microscopy, Electron