Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation.
Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG.
Ingelbrecht
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I
Laboratorium voor Genetica, Universiteit Gent, Belgium.
Van Houdt
H
H
Van Montagu
M
M
Depicker
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A
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D017796:Caulimovirus; D002467:Cell Nucleus / Q000378:metabolism; D017931:DNA Primers; D004269:DNA, Bacterial / Q000378:metabolism; D004275:DNA, Ribosomal / Q000378:metabolism; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008745:Methylation; D008969:Molecular Sequence Data; D018515:Plant Leaves; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012323:RNA Processing, Post-Transcriptional; D012231:Rhizobium; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C036483:T-DNA