Improved discrimination of bovine class II DR beta-chains polymorphisms using immunoblotting.
An immunoblotting technique is reported that reveals electrophoretic variants in the beta-chains of class II antigens of the bovine major histocompatibility complex. One monoclonal antibody, mAb VPM57, reacted on immunoblots with an epitope present in approximately half of the haplotypes investigated. This reagent is especially useful in discriminating electrophoretic variants that have similar isoelectric points.
Knowles
G
G
Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, University of Edinburgh, UK.
Dutia
B M
BM
Glass
E J
EJ
MacCarthy-Morrogh
L
L
Spooner
R L
RL
Hopkins
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle / Q000235:genetics* / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006579:Heterozygote; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000302:isolation & purification; D006720:Homozygote; D015151:Immunoblotting / Q000379:methods / Q000662:veterinary*; D007525:Isoelectric Focusing; D011110:Polymorphism, Genetic
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II