Polymorphisms detected by random PCR distinguish between different chromosomal forms of Anopheles gambiae.
We have applied PCR amplification using random primers to distinguish between incipient species of the malaria vector Anopheles gambiae. Individuals belonging to three chromosomally characterized West African forms of this mosquito, which are important epidemiologically as they differ in vectorial capacity, were sampled both from laboratory stocks and from wild populations collected in three localities. The techniques used allowed for the unambiguous classification of the mosquitoes, providing a tool for rapid and efficient diagnosis, which previously relied on cytological examination of polytene chromosomes.
Favia
G
G
Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology--Hellas, Heraklion, Crete, Greece.
Dimopoulos
G
G
della Torre
A
A
Touré
Y T
YT
Coluzzi
M
M
Louis
C
C
D000354:Africa, Western; D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D006801:Humans; D007621:Karyotyping; D008288:Malaria / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic
D017931:DNA Primers; D004247:DNA