title: Characterization of a salmon insulin-like growth factor I promoter.
abstract: We have identified four transcription initiation sites in the salmon insulin-like growth factor I (IGF-I) gene. Use of the most upstream transcription start site generates a minor mRNA species with a 5' untranslated region (UTR) of approximately 450-550 nucleotides, whereas transcription starting at the downstream initiation sites results in more abundant IGF-I mRNAs with 5'-UTRs approximately 250, 245, and 165 nucleotides in length. No consensus TATA box-like elements are present immediately upstream of the most upstream start site identified, nor is this region particularly GC-rich. Transient expression assays, however, demonstrated orientation-dependent promoter activity in a 386-nucleotide-long fragment containing the major downstream transcription start sites. Additionally, reverse transcriptase polymerase chain reaction (RT-PCR) analyses demonstrated tissue and developmental stage-specific use of the various transcription start sites identified.
authors.affiliation: Department of Biosynthesis of Nucleic Acids, Ukrainian Academy of Sciences, Kiev.
authors.forename: A
authors.initials: A
authors.lastname: Koval
authors.forename: V
authors.initials: V
authors.lastname: Kulik
authors.forename: S
authors.initials: S
authors.lastname: Duguay
authors.forename: E
authors.initials: E
authors.lastname: Plisetskaya
authors.forename: M L
authors.initials: ML
authors.lastname: Adamo
authors.forename: C T
authors.initials: CT
authors.lastname: Roberts
authors.forename: D
authors.initials: D
authors.lastname: Leroith
authors.forename: V
authors.initials: V
authors.lastname: Kavsan
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D018076:DNA, Complementary; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008969:Molecular Sequence Data; D017684:Oncorhynchus kisutch; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I