Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae.
Membrane-derived oligosaccharides (MDO) of Escherichia coli are representative members of a family of glucans found in the periplasmic space of Gram-negative bacteria. The two genes forming the mdoGH operon are necessary for the synthesis of MDO. The nucleotide sequence (4759 bp) and the transcriptional start of this operon were determined. Both gene products were further characterized by gene fusion analysis. MdoG is a 56 kDa periplasmic protein whose function remains to be determined. MdoH, whose presence was shown to be necessary for normal glucosyl transferase activity, is a 97 kDa protein spanning the cytoplasmic membrane. To our surprise, these proteins are not homologous to the periplasmic glucan biosynthetic enzymes previously characterized in the Rhizobiaceae family. However, a considerable homology (69% identical nucleotides out of 2816) was discovered between mdoGH and the two genes present at the hrpM locus of the phytopathogenic bacterium Pseudomonas syringae pv. syringae. Functions of these genes remain mysterious but they are known to be required for both the expression of disease symptoms on host plants and the development of the hypersensitive reaction on non-host plants (Mills and Mukhopadhyay, 1990). These results confirm the importance of periplasmic glucans for the physiological ecology of Gram-negative bacteria.
Loubens
I
I
Laboratoire de Chimie Biologique, UMR 111 du CNRS, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Debarbieux
L
L
Bohin
A
A
Lacroix
J M
JM
Bohin
J P
JP
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D007887:Fabaceae / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D005936:Glucans / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D009997:Osmotic Pressure; D033903:Periplasmic Proteins; D010946:Plants, Medicinal; D011549:Pseudomonas / Q000235:genetics / Q000472:pathogenicity; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D005936:Glucans; C089832:HrpM protein, Pseudomonas syringae; C089830:MdoG protein, E coli; D008565:Membrane Proteins; D033903:Periplasmic Proteins; C089831:opgH protein, E coli