[Grouping of the genes of biosynthesis and resistance to beta-lactam antibiotics in cephamycin-C producing actinomycetes].
Three genes related to beta-lactam resistance have been found in the cluster of genes for cephamycin C biosynthesis in Nocardia lactamdurans. The cmcT gene encodes a hydrophobic protein located in the cytoplasmic membrane. The sequence of CMCT has a 21-31% identity in amino acids to proteins involved in antibiotic export from other antibiotic producing microorganisms. The pbp gene encodes a penicillin binding protein. Nocardia lactamdurans is rather sensitive to penicillins, but no to cephalosporins or cephamycin C. A third gene, bla, encodes a type A beta-lactamase. Both the beta-lactamase and the PBP protein, might form a system for the sensing and hydrolysis of penicillin intermediates which are released into the medium during the lysis of antibiotic producing cells.
Liras
P
P
Facultad de Biología, Universidad de León.
Coque
J J
JJ
García-Calzada
J
J
Pérez Llarena
F J
FJ
Cardoza
R E
RE
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics; D002513:Cephamycins / Q000096:biosynthesis*; D006602:Hexosyltransferases; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics; D009615:Nocardia / Q000235:genetics* / Q000378:metabolism; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D002513:Cephamycins; C090298:CmcT protein, Nocardia lactamdurans; D008565:Membrane Proteins; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D001618:beta-Lactamases; C024925:cephamycin C
Agrupación de los genes de biosíntesis y resistencia a antibióticos beta-lactámicos en actinomicetos productores de cefamicina C.