Expression of mRNA and steady-state levels of protein isoforms of enoyl-ACP reductase from Brassica napus.
The expression of mRNA and the steady-state levels of two-component enzymes of plant fatty acid synthetase (FAS) were studied. Northern analysis of enoyl-ACP reductase (ER) and stearoyl-ACP desaturase (SD) gene expression showed that steady-state levels of both transcripts increase during lipid deposition in the seed reaching a maximum at 29 days after flowering (DAF). The steady-state level of ER message falls very quickly after reaching its maximum, whereas the SD message is longer-lived. The levels of these specific mRNAs in seed are 15-30 times greater than in leaf. Optimum mRNA expression precedes the maximum levels of synthesis of the two proteins, which in turn precede the maximum level of oil. The expression of isoenzymes of ER were examined by two-dimensional western blotting in both leaf and seed tissue. Four enzymes are expressed in both of these tissues; the two most abundant isoforms in seed material are also the most abundant in leaf tissue.
Fawcett
T
T
Department of Biological Sciences, University of Durham, UK.
Simon
W J
WJ
Swinhoe
R
R
Shanklin
J
J
Nishida
I
I
Christie
W W
WW
Slabas
A R
AR
D001937:Brassica / Q000201:enzymology* / Q000235:genetics; D050753:Enoyl-(Acyl-Carrier-Protein) Reductase (NADH); D064429:Fatty Acid Synthases / Q000096:biosynthesis* / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D018506:Gene Expression Regulation, Plant; D007526:Isoelectric Point; D007527:Isoenzymes / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D018749:RNA, Plant / Q000032:analysis / Q000096:biosynthesis; D012639:Seeds / Q000737:chemistry; D014158:Transcription, Genetic
D005227:Fatty Acids; D007527:Isoenzymes; D012333:RNA, Messenger; D018749:RNA, Plant; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; C018747:acyl-(acyl-carrier-protein)desaturase; D050753:Enoyl-(Acyl-Carrier-Protein) Reductase (NADH); D064429:Fatty Acid Synthases