A rapid colorimetric assay for determination of leukocyte-mediated inhibition of mycelial growth of Candida albicans.
A staining method with crystal violet (CV) was demonstrated to be useful for a simple, quick and objective assessment of in vitro growth inhibitory activity of leukocytes against Candida albicans cells. Candida cells incubated with murine neutrophils or macrophages for 14 hr in microwells were stained with CV and, after washing with 0.25% sodium dodecyl sulfate (SDS), treated with isopropanol containing HCl (0.04 N) to extract Candida cell-bound CV. Then the absorbance at 590 nm of the isopropanol extract was photometrically measured. The results showed that the photometrical absorbance was proportional to the amount of 3H-glucose taken up by C. albicans cells, which reflected the number of viable Candida cells.
Abe
S
S
Department of Microbiology and Immunology, School of Medicine, Teikyo University, Tokyo, Japan.
Satoh
T
T
Tokuda
Y
Y
Tansho
S
S
Yamaguchi
H
H
D000818:Animals; D002176:Candida albicans / Q000254:growth & development / Q000276:immunology*; D003124:Colorimetry / Q000379:methods*; D005840:Gentian Violet; D005947:Glucose / Q000378:metabolism; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D009504:Neutrophils / Q000276:immunology*
D005947:Glucose; D005840:Gentian Violet