Interleukin-4 induces in vitro migration in naive B cells through direct mechanisms.
Interleukin-4 (IL-4) mediated locomotor responses by murine B cells in vitro were examined in this paper. The IL-4 induced migration was found to act directly on purified, splenic B cells, without involvement of secondary mediators. It appeared that only a subpopulation of B cells was able to respond in migration assays. Flowcytometric analysis showed that the migrating cells had the characteristics of naive B cells: I-Alo, J11dhi, IgDhi. They also displayed high expression of the adhesion molecule L-selectin and made predominantly IgM antibodies. This is contrary to what has previously been observed regarding motile responses to chemotactic factors by T cells, which mostly affect memory or activated T cells. However, this is in accordance with other studies indicating that IL-4 is a cytokine that exerts its effect mainly on resting B cells.
Clinchy
B
B
Department of Immunology, Stockholm University, Sweden.
Möller
G
G
D000818:Animals; D000917:Antibody Formation / Q000502:physiology; D015703:Antigens, CD / Q000502:physiology; D016175:B-Lymphocyte Subsets / Q000502:physiology; D001402:B-Lymphocytes / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology; D002465:Cell Movement / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D015847:Interleukin-4 / Q000502:physiology*; D008213:Lymphocyte Activation / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D015847:Interleukin-4