Modulation of phenotypic and functional properties of normal human mononuclear phagocytes by granulocyte-macrophage colony-stimulating factor.
In asthma, alveolar macrophages (AMs) are hyperreactive releasing large amounts of mediators and expressing high levels of surface markers. Granulocyte-macrophage colony-stimulating factor (GM-CSF) upregulates monocytes and AMs, and may be involved in the hyperreactivity of AMs. The effects of GM-CSF were tested on monocytes and AMs from normal subjects by examining the expression of factors thought to be upregulated in asthma. After various incubation times of GM-CSF, the expression of CD23 and beta 2-integrins (CD11a, CD11b, CD11c) was studied by FACS and the release of sCD23 was measured by ELISA. The priming and stimulatory effects of GM-CSF were tested on monocytes and AMs and the release of leukotriene B4 (LTB4) was measured by ELISA. GM-CSF induced the expression of CD11c and CD23 and the release of sCD23. GM-CSF primed and stimulated monocytes and AMs to release LTB4. The effects of GM-CSF may explain partly the hyperreactivity of AMs in asthma.
Rivier
A
A
CJF INSERM 92-10, Hôpital Arnaud-de-Villeneuve, Montpellier, France.
Chanez
P
P
Pène
J
J
Michel
F B
FB
Godard
P
P
Dugas
B
B
Bousquet
J
J
D000328:Adult; D000368:Aged; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002478:Cells, Cultured; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology*; D006801:Humans; D016130:Immunophenotyping; D016023:Integrins / Q000096:biosynthesis; D007975:Leukotriene B4 / Q000096:biosynthesis; D016676:Macrophages, Alveolar / Q000276:immunology*; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D017455:Receptors, IgE / Q000096:biosynthesis
D015815:Cell Adhesion Molecules; D016023:Integrins; D017455:Receptors, IgE; D007975:Leukotriene B4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor