title: Identification of the ron gene product as the receptor for the human macrophage stimulating protein.
abstract: Macrophage-stimulating protein (MSP) is a member of the hepatocyte growth factor-scatter factor (HGF-SF) family. Labeled MSP bound to Madin-Darby canine kidney (MDCK) cells transfected with complementary DNA encoding Ron, a cell membrane protein tyrosine kinase. Cross-linking of 125I-labeled MSP to transfected cells (MDCK-RE7 cells) and immunoprecipitation by antibodies to Ron revealed a 220-kilodalton complex, a size consistent with that of MSP (80 kilodaltons) cross-linked to the beta chain of Ron (150 kilodaltons). The binding of 125I-labeled MSP to MDCK-RE7 cells was inhibited by unlabeled MSP, but not by HGF-SF. MSP caused phosphorylation of the beta chain of Ron and induced migration of MDCK-RE7 cells. These results establish the ron gene product as a specific cell-surface receptor for MSP.
authors.affiliation: Immunopathology Section, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702.
authors.forename: M H
authors.initials: MH
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Ronsin
authors.forename: M C
authors.initials: MC
authors.lastname: Gesnel
authors.forename: L
authors.initials: L
authors.lastname: Coupey
authors.forename: A
authors.initials: A
authors.lastname: Skeel
authors.forename: E J
authors.initials: EJ
authors.lastname: Leonard
authors.forename: R
authors.initials: R
authors.lastname: Breathnach
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D003432:Cross-Linking Reagents; D004285:Dogs; D006133:Growth Substances / Q000378:metabolism* / Q000494:pharmacology; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation; D010958:Plasminogen / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D003432:Cross-Linking Reagents; D006133:Growth Substances; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; C082332:macrophage stimulating protein; D017228:Hepatocyte Growth Factor; D010958:Plasminogen; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases