title: Glucocorticoid induction of rat angiotensin II type 1A receptor gene promoter.
abstract: Promoter/luciferase reporter analysis indicated that the 5'-flanking region of rat angiotensin II type 1A receptor gene was functional, both in the rat aortic smooth muscle cells (RASMCs) and in Y1 cells derived from mouse adrenal cortex. In response to dexamethasone (Dex), transcriptional activity of promoter increased in transfected RASMCs, suggesting the functional cis-action of a glucocorticoid responsive elements (GRE). Furthermore, the expression of the rat AT1A gene was also up-regulated by Dex at the levels of both mRNA and protein in RASMCs.
authors.affiliation: Research Div., Green Cross Corp., Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Uno
authors.forename: D F
authors.initials: DF
authors.lastname: Guo
authors.forename: M
authors.initials: M
authors.lastname: Nakajima
authors.forename: H
authors.initials: H
authors.lastname: Ohi
authors.forename: T
authors.initials: T
authors.lastname: Imada
authors.forename: R
authors.initials: R
authors.lastname: Hiramatsu
authors.forename: H
authors.initials: H
authors.lastname: Nakakubo
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Inagami
mesh_terms: D000306:Adrenal Cortex Neoplasms; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D008156:Luciferases / Q000096:biosynthesis; D008297:Male; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011945:Receptors, Angiotensin / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D011994:Recombinant Proteins; D000804:Angiotensin II; D003907:Dexamethasone; D008156:Luciferases