Post-junctional excitatory adenosine A1 receptors in the rat vas deferens.
1. At concentrations between 1 nM and 1 microM, the A1-selective agonists N6-cyclopentyladenosine (CPA) and (R)-N6-phenylisopropyladenosine (R-PIA) each enhanced contractions of the rat vas deferens induced by ATP (10 microM), and this enhancement was blocked by an A1-selective concentration (1 nM) of the antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX). 2. No such enhancement was observed with the non-selective agonists adenosine and 5'-N-ethylcarboxamidoadenosine (NECA) at concentrations between 1 nM and 100 microM, which instead inhibited the contractions. 3. These results show that in addition to the previously demonstrated inhibitory A1 and A2 adenosine receptors, the rat vas deferens also possesses post-junctional excitatory A1 adenosine receptors.
Hourani
S M
SM
Receptors and Cellular Regulation Research Group, School of Biological Sciences, University of Surrey, Guildford, England.
Jones
D A
DA
D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects*; D009924:Organ Culture Techniques; D010660:Phenylisopropyladenosine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D018047:Receptors, Purinergic P1 / Q000187:drug effects* / Q000378:metabolism; D014649:Vas Deferens / Q000187:drug effects* / Q000378:metabolism
D018047:Receptors, Purinergic P1; D010660:Phenylisopropyladenosine; C048599:N(6)-cyclopentyladenosine; D000241:Adenosine