[Electron microscopic observation on intracellular connective structures during healing of the tracheal mucous epithelia of guinea pigs--by means of tissue culture].
Healing processes of the tracheal epithelia after minor mechanical injury in vitro were morphologically investigated by electron microscopy. Actin filaments in the epithelia, at the injured site, were observed by phalliodin. The results are as follows: 1) The injured area was covered with one layer of epithelium within 48 hours and there were no mitotic figures. 2) The epithelial cells at the healing site were squamous and well developed. 3) Actin-filaments could be observed with well developed interdigitation. In conclusion these findings suggest that areas of minor injury heal by sliding of cells surrounding the injured area. The development of interdigitation is considered to play an important role in this process. It seems that actin observed at the site of interdigitation development plays some role in this healing process.
Hamai
Y
Y
Department of Otorhinolaryngology, Hiroshima University School of Medicine.
D000818:Animals; D046508:Culture Techniques; D004848:Epithelium / Q000293:injuries / Q000648:ultrastructure; D005110:Extracellular Space; D006168:Guinea Pigs; D008854:Microscopy, Electron; D009092:Mucous Membrane / Q000293:injuries / Q000648:ultrastructure; D014132:Trachea / Q000293:injuries* / Q000648:ultrastructure*; D014945:Wound Healing