title: The two-hybrid system: an assay for protein-protein interactions.
abstract: The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. It can be used to identify proteins that bind to a protein of interest, or to delineate domains or residues critical for an interaction. Variations on this methodology have been developed to clone genes that encode DNA-binding proteins, to identify peptides that bind to a protein and, potentially, to screen for drugs.
authors.affiliation: Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook 11794.
authors.forename: S
authors.initials: S
authors.lastname: Fields
authors.forename: R
authors.initials: R
authors.lastname: Sternglanz
mesh_terms: D004268:DNA-Binding Proteins / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D011506:Proteins