title: Evaluation of in vivo and in vitro interactions of feline immunodeficiency virus and feline leukemia virus.
abstract: OBJECTIVE
  To determine the potential mechanisms for disease potentiation where feline immunodeficiency virus (FIV) infection of persistently feline leukemia virus (FeLV)-infected cats results in more severe FIV disease and increased mortality than FIV infection of specific pathogen-free cats.
  
  
  METHODS
  To determine whether pseudotype formation resulting in expanded cell tropism may be an important mechanism, cellular targets and tissue distribution of FIV and FeLV were determined by in situ hybridization and/or immunohistochemistry. To determine whether FeLV can transactivate the FIV long terminal repeat (LTR) resulting in increased FIV expression, in vitro transient expression assays were performed. To examine whether persistent FeLV infection can cause the deletion of a suppressive T-lymphocyte population, peripheral blood mononuclear cell (PBMC) cultures from persistently FeLV-infected cats were infected with FIV and monitored for FIV antigen levels.
  
  
  RESULTS
  Macrophages were the predominant target of FIV infection and were disseminated in a similar pattern in lymphoid and nonlymphoid tissues of both FIV-infected and FeLV/FIV-coinfected cats. FeLV-infected cells expressing FIV RNA were not present. Significant transactivation of the FIV LTR in FeLV-infected cells was not demonstrated. FIV antigen production was similar upon in vitro infection of PBMC from FeLV-infected and uninfected cats.
  
  
  CONCLUSIONS
  Neither direct virus/virus interactions, such as FeLV/FIV pseudotype formation or transactivation of the FIV LTR in FeLV-infected cells, nor deletion of a regulatory cell subset from the blood of FeLV-infected cats, was found to be the mechanism of disease potentiation.
authors.affiliation: Department of Internal Medicine, School of Medicine, University of California, Davis 95616.
authors.forename: A M
authors.initials: AM
authors.lastname: Beebe
authors.forename: T G
authors.initials: TG
authors.lastname: Faith
authors.forename: E E
authors.initials: EE
authors.lastname: Sparger
authors.forename: M
authors.initials: M
authors.lastname: Torten
authors.forename: N C
authors.initials: NC
authors.lastname: Pedersen
authors.forename: S
authors.initials: S
authors.lastname: Dandekar
mesh_terms: D000818:Animals; D002415:Cats; D002478:Cells, Cultured; D016181:Feline Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000382:microbiology; D015967:Gene Expression Regulation, Viral; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000502:physiology*; D017403:In Situ Hybridization; D005256:Leukemia Virus, Feline / Q000235:genetics / Q000502:physiology*; D016582:Leukemia, Feline / Q000150:complications* / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D012367:RNA, Viral / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000382:microbiology; D015533:Transcriptional Activation; D014762:Viral Interference
chemical_list: D012367:RNA, Viral