Pulmonary response of Fischer 344 rats to acute nose-only inhalation of indium trichloride.
We have previously shown that rats dosed intratracheally with indium trichloride (InCl3) develop severe lung damage and fibrosis. However, it is not clear what pulmonary effects would result following accidental occupational exposure to low concentrations of indium by inhalation. The present study uses a model of acute lung injury based on single 1-hr nose-only exposures to 0.2, 2.0, or 20 mg InCl3/m3. Exposure to 0.2 mg InCl3/m3 was capable of initiating an inflammatory response. Seven days following inhalation of 20 mg InCl3/m3 the total cell number, fibronectin, and TNF alpha levels in the bronchial alveolar lavage fluid were 8, 40, and 5 times higher than the control, respectively. Commensurate with the level of lung injury 7 days after exposure, an acute restrictive lung lesion and increased airway responsiveness to acetylcholine were observed. Forty-two days after exposure a compensatory increase in lung volume and carbon monoxide diffusing capacity in the 20 mg InCl3/m3 group suggested recovery from the lung injury. Lung collagen levels were increased in a concentration-dependent manner 42 days postexposure. These data indicate that inhalation of InCl3/m3 causes acute inflammatory changes in the lung.
Blazka
M E
ME
Environmental Immunology and Neurobiology Section, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Tepper
J S
JS
Dixon
D
D
Winsett
D W
DW
O'Connor
R W
RW
Luster
M I
MI
D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D005260:Female; D005353:Fibronectins / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D007204:Indium / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*; D008168:Lung / Q000737:chemistry / Q000187:drug effects* / Q000503:physiopathology; D015999:Multivariate Analysis; D009929:Organ Size / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D012129:Respiratory Function Tests; D014109:Total Lung Capacity / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D000336:Aerosols; D005353:Fibronectins; D014409:Tumor Necrosis Factor-alpha; D007204:Indium; C020758:indium trichloride; D006909:Hydroxyproline