The in vivo and in vitro effects of antibodies against rabbit beta 2-integrins.
Beta 2-integrins play a crucial role in the development of an inflammatory response. In ours study, Abs have been used to investigate the role of individual members of this family of adhesion molecules in both in vivo and in vitro assays. An Ab against rabbit LFA-1 effectively inhibited the adhesion of rabbit polymorphonuclear leukocytes to rabbit endothelial cells in culture and was also effective in blocking cell recruitment to the peritoneum and vascular leakage at dermal sites of inflammation. An Ab that inhibited rabbit complement receptor type 3 function in vitro failed to inhibit cell recruitment to the peritoneum or vascular leakage in response to intradermal FMLP. Histologic studies suggested that the anti-complement receptor type 3 Ab may have modified the cell migration process.
Rutter
J
J
Department of Inflammation Biology, Celltech Ltd., Slough, Berkshire, UK.
James
T J
TJ
Howat
D
D
Shock
A
A
Andrew
D
D
De Baetselier
P
P
Blackford
J
J
Wilkinson
J M
JM
Higgs
G
G
Hughes
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D001920:Bradykinin / Q000494:pharmacology; D018821:CD18 Antigens / Q000502:physiology*; D002199:Capillary Permeability; D002448:Cell Adhesion; D002478:Cells, Cultured; D003872:Dermatitis / Q000503:physiopathology; D066298:In Vitro Techniques; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D016177:Macrophage-1 Antigen / Q000502:physiology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D009240:N-Formylmethionine Leucyl-Phenylalanine; D001920:Bradykinin