The combined use of soybean agglutinin and immunomagnetic beads for T lymphocyte subset depletion of bone marrow allografts: a laboratory analysis.
The prevention of graft-versus-host disease by T-lymphocyte depletion of allografts prior to bone marrow transplantation has resulted in an increase in graft failure/rejection and relapse of disease. Evidence for the selective roles of specific T-lymphocyte subsets in each of the engraftment, graft-versus-host disease, and disease relapse processes has been presented, but sufficient clinical verification to support any hypothesis in this regard is lacking. In this paper we describe a convenient and highly flexible clinical laboratory method for depletion of specific T-lymphocytes in controllable quantities by the use of select monoclonal antibodies. Almost 3 log10 removal of CD2+ and CD8+ cells without significant loss of hematopoietic progenitors (CFU-GM, BFU-E, and CD34+ cells) can be reproducibly achieved. This method, employing soybean agglutination and immunomagnetic beads, is potentially adaptable to depletion of any cell subset or any tumor cell for which unique cell-surface antigen characteristics have been defined. In addition, our protocol is equally suited to the positive selection of stem cells and hematopoietic progenitors bearing the CD34 surface antigen.
Preti
R A
RA
Hudson Valley Blood Services, Division of the New York Blood Center, Valhalla 10595.
Farley
T J
TJ
Fan
Y
Y
Ahmed
T
T
Rose
M
M
Ciavarella
D
D
D016026:Bone Marrow Transplantation; D018801:CD2 Antigens / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D006801:Humans; D018189:Immunomagnetic Separation; D007753:Laboratories; D037102:Lectins; D018655:Lymphocyte Count; D037121:Plant Lectins; D030262:Soybean Proteins; D013025:Glycine max; D016176:T-Lymphocyte Subsets / Q000166:cytology*; D014184:Transplantation, Homologous; D010835:Phytohemagglutinins
D018801:CD2 Antigens; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins