Effects of trifluoromethylaniline isomers on enzyme activities in lymphatic organs and hematology of the rat.
Three isomers of trifluoromethylaniline (TFMA) were investigated for their possible different toxic effects on the hematopoietic system in male Wistar rats. The effects of isomeric 2-, 3- and 4-TFMA were compared with those of aniline, the prototypic drug. Strong leukocytosis manifested by considerable increase in the number of all respective white blood elements was observed in the peripheral blood 1 day after the administration of 4-TFMA. In contrast, erythropoiesis, as ascertained by erythrocyte count and hemoglobin concentration, was inhibited by 4-TFMA. The determination of the ED50 revealed lymphocytes to be the most responsive elements towards 4-TFMA administration. Besides hyperemic and proliferative splenomegaly the histological changes in maturation of immunocompetent cells following the 4-TFMA administration were found also in thymus. In accord with an enhanced incorporation of [3H]thymidine, the specific activity of thymidine kinase (TdK) in spleen was increased after a single dose of 4-TFMA. Activities of the catabolic enzymes adenosine deaminase (ADA) and inosine phosphorylase (IP) decreased in both organs with the exception of IP activity in thymus. The effects evoked by the 3-TFMA isomer were regularly less pronounced, and 2-TFMA was nearly inactive.
Sebestová
L
L
Institute of Pharmacology, Academy of Sciences of the Czech Republic, Prague.
Seifert
J
J
Jiricka
Z
Z
Kolar
G F
GF
D000243:Adenosine Deaminase / Q000187:drug effects; D000814:Aniline Compounds / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D001772:Blood Cell Count / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006410:Hematopoiesis / Q000187:drug effects*; D007536:Isomerism; D008221:Lymphoid Tissue / Q000187:drug effects* / Q000201:enzymology* / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011683:Purine-Nucleoside Phosphorylase / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013937:Thymidine Kinase / Q000187:drug effects
D000814:Aniline Compounds; C487557:4-trifluoromethylaniline; D011683:Purine-Nucleoside Phosphorylase; D013937:Thymidine Kinase; D000243:Adenosine Deaminase; C023650:aniline