Enhancement of the responsiveness of vas deferens and ileum smooth muscle in sensitized guinea pigs: in vitro study.
Changes in the reactivity of the ileum (to histamine and barium chloride) and vas deferens (to acetylcholine and barium chloride), isolated from actively egg albumen-sensitized guinea pigs, have been investigated. The study was performed on 2 guinea pig strains: the Dunkin-Hartley strain, usually used as an airway allergic model, and the BFA strain. In actively sensitized guinea pigs of both strains, concentration-response curves exhibited a significant dose-dependent upward shift compared to those obtained in control guinea pigs. The maximal contraction strength calculated from these curves was significantly enhanced in both sensitized guinea pig strains, without a change in EC50 values. This study showed that the active antigen sensitization procedure involved several smooth muscle functions, and not exclusively the trachea.
Bidon
J C
JC
Laboratoire de Physiologie et Pharmacodynamie, Institut national de la Recherche agronomique, Ecole nationale vétérinaire, Nantes, France.
Blin
M
M
Gogny
M
M
Vu
A T
AT
Jondet
A
A
D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D017609:Barium Compounds / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D006168:Guinea Pigs; D006632:Histamine / Q000502:physiology; D007082:Ileum / Q000187:drug effects / Q000276:immunology; D007114:Immunization; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000276:immunology; D009130:Muscle, Smooth / Q000187:drug effects* / Q000276:immunology*; D014649:Vas Deferens / Q000187:drug effects / Q000276:immunology
D017609:Barium Compounds; D002712:Chlorides; C024986:barium chloride; D006632:Histamine; D000109:Acetylcholine