Expression of F4, 8.12, 3I, and 16/6 anti-DNA idiotype-related antigens on cationic human IgG myeloma proteins.
One hundred cationic isolated human IgG myeloma proteins were studied for expression of four anti-DNA idiotypic (Id) markers F4, 3I, 8.12, and 16/6. Forty-three of 100 myelomas showed the presence of at least one anti-DNA idiotype. Twenty-seven were F4 positive, 18 were 16/6 positive, 8 were 3I positive, and 7 were positive for 8.12. Two different anti-DNA idiotypic markers were found together on the same 14 myeloma proteins and 3 myelomas showed three different anti-DNA Ids. Anti-DNA activity was found on only 1 of 100 myelomas but not associated with presence of an anti-DNA Id. Ten myelomas showed anti-F(ab')2 activity and 6 of these also showed the presence of an anti-DNA Id marker. When myeloma proteins expressing anti-DNA Ids were compared in direct competition ELISAs with known Id-positive human IgG anti-DNA antibodies, much less inhibition was recorded in comparison to known human anti-DNA antibodies. Our findings indicate that human IgG myeloma proteins may show positive reactions with anti-DNA idiotypic antibodies but do not express the complete anti-DNA idiotypic antigenic complex.
Williams
R C
RC
Department of Medicine, University of Florida School of Medicine, Gainesville 32610.
Schriber
A D
AD
Malone
C C
CC
Silvestris
F
F
Hannigan
N
N
Klein-Gitelman
M
M
Namyst
C
C
Kyle
R A
RA
D000974:Antibodies, Antinuclear / Q000502:physiology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000032:analysis; D002412:Cations; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D009194:Myeloma Proteins / Q000737:chemistry / Q000276:immunology*
D000974:Antibodies, Antinuclear; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002412:Cations; D007130:Immunoglobulin Idiotypes; D009194:Myeloma Proteins