title: Modification of the radiosensitivity of human testicular cancer cells by simian virus 40 sequences.
abstract: CRL7800 cells are of human testicular cancer origin and are sensitive to killing by ionizing radiation. After transfection with a plasmid expressing the T-antigen (pSC), cells show enhanced growth and an increased resistance to ionizing radiation. Cell cycle analysis reveals perturbation of a cell cycle checkpoint which, after irradiation, results in an increase in G2-phase arrest in CRL7800VA cells. These experiments demonstrate the modulation of radiation sensitivity and cell cycle arrest of human tumor cells by the introduction of viral genes.
authors.affiliation: Department of Radiation Medicine, Georgetown University School of Medicine, Washington, D.C. 20007.
authors.forename: M
authors.initials: M
authors.lastname: Jung
authors.forename: A
authors.initials: A
authors.lastname: Dritschilo
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002470:Cell Survival / Q000528:radiation effects; D016195:G2 Phase; D006801:Humans; D008297:Male; D010957:Plasmids; D011836:Radiation Tolerance; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D013736:Testicular Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D014162:Transfection; D016662:Virus Integration
chemical_list: D000952:Antigens, Polyomavirus Transforming