Bcl-2 overexpression abolishes early calcium waving preceding apoptosis in NIH-3T3 murine fibroblasts.
Overexpression of the apoptosis protection gene bcl-2 abolished the earliest response to serum withdrawal in NIH-3T3 murine fibroblasts, i.e., the abrupt cytoplasmic free calcium drop. This phenomenon, also observed in a myeloid cell line, led us to propose this ionic waving as an early apoptotic signal. Its abolition by bcl-2 overexpression suggests that this gene plays a role also on early events "priming" apoptosis.
Magnelli
L
L
Laboratory of Molecular Biology, University of Florence, Italy.
Cinelli
M
M
Turchetti
A
A
Chiarugi
V P
VP
D016475:3T3 Cells; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D016895:Culture Media, Serum-Free; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D019204:GTP-Binding Proteins / Q000096:biosynthesis; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019253:Proto-Oncogene Proteins c-bcl-2; D013997:Time Factors; D014162:Transfection
D016895:Culture Media, Serum-Free; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D019204:GTP-Binding Proteins; D002118:Calcium