Productions of interferon-gamma and nitrite are induced in mouse splenic cells by a heteroglycan-protein fraction from culture medium of Lentinus edodes mycelia.
A xylose-rich heteroglycan-protein fraction (LAP1) was prepared from a solid culture medium in which Lentinus edodes mycelia were growing actively. Mouse splenic cells (SPs) were incubated with [3H]TdR in the presence of LAP1. The incubated SPs were fractionated into plastic adherent splenic cells (ADs), nylon-column effluent splenic cells (NEs) and sIg-expressed splenic cells (SIs), which are rich in Mac-1+, Thy-1.2+ and Ly-5+ cells, respectively. The incorporation of [3H]TdR in response to LAP1 was enhanced in each of the fractionated cell populations. Northern or dot blot hybridization showed that productions of IFN-gamma and its receptor mRNAs are induced predominantly in NEs. In another experiment, SPs were fractionated into ADs, NEs and SIs. Then, NE-AD, SI-AD and NE-SI mixtures were prepared and incubated in the same manner. A significant incorporation of [3H]TdR was shown only in the NE-AD mixture. The enzyme-linked immunosorbent assay showed that IFN-gamma production in response to LAP1 is induced in SPs or in the NE-AD mixture, but not in NEs alone. The level of the production was about 5 times higher in the mixture than in SPs after a 72 h incubation. Moreover, LAP1 was capable of inducing NO2- production in SPs. Thus, the present studies imply that this heteroglycan-protein fraction stimulates productions of IFN-gamma and nitrite in mouse splenic cells, augmenting antitumor immune response(s).
Hibino
Y
Y
Cell Biology Division, Faculty of Pharmaceutical Sciences, Toyama Medical & Pharmaceutical University, Japan.
Konishi
Y
Y
Koike
J
J
Tabata
T
T
Ohashi
Y
Y
Sugano
N
N
D000818:Animals; D015152:Blotting, Northern; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000032:analysis / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000032:analysis / Q000494:pharmacology; D009573:Nitrites / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011124:Polyporaceae / Q000737:chemistry*; D011509:Proteoglycans / Q000032:analysis / Q000494:pharmacology*; D012331:RNA, Fungal; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D005656:Fungal Proteins; D008934:Mitogens; D009573:Nitrites; D011509:Proteoglycans; D012331:RNA, Fungal; D007371:Interferon-gamma