Ubiquitin genes and ubiquitin protein location in polytene chromosomes of Drosophila.
Ubiquitin genes are found in Drosophila either as a repeat block or as gene fusions with ribosomal proteins. Here is described the location of a new repeat block in the X chromosome that is present in the strain Canton S but absent in Vallecas. There are also two ubiquitin-ribosomal protein fusion genes located at regions 97A of chromosome 3R and 31E of 2L. Using an anti-ubiquitin antibody in Drosophila polytene chromosomes it is shown that ubiquitin is mainly associated with the compact and stabilized structure that forms the bands rather than with the more decondensed and destabilized protein-DNA structure that forms interbands and puffs.
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Departamento de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, Spain.
D000818:Animals; D001704:Biopolymers / Q000032:analysis / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes / Q000737:chemistry* / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics*; D025821:Polyubiquitin; D012469:Salivary Glands; D014452:Ubiquitins / Q000032:analysis* / Q000235:genetics*; D014960:X Chromosome
D001704:Biopolymers; D004275:DNA, Ribosomal; D014452:Ubiquitins; D025821:Polyubiquitin