Modulation of cytokine expression in mouse dendritic cell clones.
Dendritic cells (DC) play an essential role in the induction of primary immune responses; however, very little information is available on cytokine production by DC. Here we determined the cytokine gene expression profile of two immortalized DC clones, CB1 and D2SC/1, both generated from mouse spleen but differing in their activation requirements. Among the cytokines tested, only transforming growth factor-beta 1 was transcribed constitutively, but its production was detected only in D2SC/1 cells after treatment with granulocyte/macrophage colony-stimulating factor (GM-CSF). GM-CSF also promoted transcription and synthesis of interleukin (IL)-1 beta in CB1 cells that need pretreatment with GM-CSF to present major histocompatibility complex class II-restricted antigens efficiently in vitro. Lipopolysaccharide (LPS) up-regulated gene expression and induced release of tumor necrosis factor-alpha in both DC clones. In addition, LPS induced transcription of IL-1 alpha and both gene expression and synthesis of IL-1 beta in D2SC/1 cells. Interferon-gamma was ineffective in inducing cytokine gene expression, although it augmented the antigen-presentation capacity of DC, IL-4, IL-10 and IL-12 mRNA were not induced by any of the tested stimuli. The results suggest that DC have a limited cytokine gene expression pattern compared to macrophages and are heterogenous in some functional properties.
Granucci
F
F
Department of Pharmacology, CNR Center of Cytopharmacology, University of Milan, Italy.
Girolomoni
G
G
Lutz
M B
MB
Foti
M
M
Marconi
G
G
Gnocchi
P
P
Nolli
L
L
Ricciardi-Castagnoli
P
P
D000818:Animals; D001483:Base Sequence; D002999:Clone Cells; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
D016207:Cytokines; D017931:DNA Primers; D012333:RNA, Messenger