Subcellular boron-10 localization in glioblastoma for boron neutron capture therapy with Na2B12H11SH.
Because of the short range of the highly energetic particles helium-4 and lithium-7 that results from neutron-induced disintegration of boron-10, the efficacy of Boron Neutron Capture Therapy (BNCT) is heavily dependent on 10B-microlocation. Despite the crucial importance of boron-10, there is little specific information with regard to the agent currently used for inducing BNCT, namely Na2B12H11SH. In the present study, a subcellular 10B-location was investigated in tumor tissue obtained from seven patients with glioblastoma World Health Organization Grade IV. These patients received Na2B12H11SH at doses used in therapeutic trials (75 mg/kg body weight in five patients, and 150 mg/kg body weight in two patients, respectively). In three cases, boron-10 was identified in glioblastoma cells by laser microprobe mass analysis. In these tumors, boron-10 was found only in the nuclei of neoplastic cells but not in other cell compartments. These preliminary results suggest a predominant association of Na2B12H11SH with the nuclei of malignant glioma cells and thus support the value of Na2B12H11SH as a suitable boron carrier for BNCT.
Haselsberger
K
K
Department of Neurosurgery, Karl Franzens University, Graz, Austria.
Radner
H
H
Gössler
W
W
Schlagenhaufen
C
C
Pendl
G
G
D001894:Borohydrides / Q000378:metabolism / Q000627:therapeutic use*; D001895:Boron / Q000378:metabolism*; D016754:Boron Neutron Capture Therapy; D001932:Brain Neoplasms / Q000378:metabolism / Q000532:radiotherapy* / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D005909:Glioblastoma / Q000378:metabolism / Q000532:radiotherapy* / Q000648:ultrastructure*; D006801:Humans; D007554:Isotopes; D007834:Lasers; D013058:Mass Spectrometry; D008854:Microscopy, Electron; D012815:Signal Processing, Computer-Assisted; D013347:Subcellular Fractions / Q000378:metabolism* / Q000648:ultrastructure; D013438:Sulfhydryl Compounds / Q000378:metabolism / Q000627:therapeutic use*
D001894:Borohydrides; D007554:Isotopes; D013438:Sulfhydryl Compounds; C014651:mercaptoundecahydrododecaborate; D001895:Boron