title: Quantification of GR90291 in human blood by high resolution gas chromatography-mass selective detection (HRGC-MSD).
abstract: A specific and sensitive high resolution gas chromatography-mass spectrometry method for the determination of GR90291 in human blood is described. The extraction of GR90291 from blood required a polar organic solvent mixture. The crude extract was further purified by successive liquid-liquid partitioning prior to esterification with an HCl-n-butanol solution. This derivative was analysed using a deuterium-labelled internal standard by selected ion monitoring mass spectrometry. The calibration curve ranged from 1 to 100 ng ml-1. The method is reliable for the determination of GR90291 pharmacokinetics in human subjects.
authors.affiliation: Phoenix International Life Sciences, Saint-Laurent, Montreal, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Lessard
authors.forename: B
authors.initials: B
authors.lastname: Comeau
authors.forename: A
authors.initials: A
authors.lastname: Charlebois
authors.forename: L
authors.initials: L
authors.lastname: Letarte
authors.forename: I M
authors.initials: IM
authors.lastname: Davis
mesh_terms: D000701:Analgesics, Opioid / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010880:Piperidines / Q000097:blood* / Q000493:pharmacokinetics; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D000701:Analgesics, Opioid; D010880:Piperidines; C084032:GI 90291