Ciliary neurotrophic factor induced-increase in beta-amyloid precursor protein mRNA in rat C6 glioma cells.
The effect of ciliary neurotrophic factor (CNTF) on beta-amyloid precursor protein (APP) gene expression was investigated in cultured rat C6 glioma cells and human SH-SY5Y neuroblastoma cells. CNTF increased APP mRNA abundance in C6 glioma cells in a dose-dependent manner, with an approximately 3-fold increase in maximum observed after 24 h with a concentration of 1 ng/ml. However, no significant differences in the splicing pattern of the three major isoforms of APP mRNA were apparent between control and CNTF-treated C6 glioma cells. CNTF had no effect on APP mRNA abundance in SH-SY5Y neuroblastoma cells. These findings suggest that CNTF can modulate APP mRNA expression and might affect amyloidogenesis in Alzheimer's disease.
Sudoh
S
S
Third Department of Internal Medicine, School of Medicine, Hiroshima University, Japan.
Kawakami
H
H
Ohta
M
M
Nakamura
S
S
D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis*; D000818:Animals; D002460:Cell Line; D020934:Ciliary Neurotrophic Factor; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D005910:Glioma; D007700:Kinetics; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009447:Neuroblastoma; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D014407:Tumor Cells, Cultured
D016564:Amyloid beta-Protein Precursor; D020934:Ciliary Neurotrophic Factor; D017931:DNA Primers; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger