title: The use of dot-immunogold-silver staining (dot-IGSS), dot-ELISA and dot-IGSS to detect serum antibodies from clonorchiasis patients: a comparative study.
abstract: We report the use of immunogold-silver staining (IGSS), dot-ELISA and dot-IGSS methods in the study of clonorchiasis in China. These methods were employed to detect the antibody in sera from 40 clonorchiasis patients. The positive rates were 100%, 90.0% and 95.0%, respectively. When the three methods were used to examine 40 normal sera, the negative rates were 100%, 97.5% and 97.5%, respectively. These results suggest that IGSS, dot-ELISA and dot-IGSS are highly specific and sensitive in detecting anti-Clonorchis antibody in patients.
authors.affiliation: Jiangsu Institute of Parasitic Diseases, Wuxi, People's Republic of China.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Wu
authors.forename: W P
authors.initials: WP
authors.lastname: Du
authors.forename: Y W
authors.initials: YW
authors.lastname: Rong
authors.forename: X H
authors.initials: XH
authors.lastname: Ji
authors.forename: S Y
authors.initials: SY
authors.lastname: Yuan
mesh_terms: D000909:Antibodies, Helminth / Q000302:isolation & purification*; D003003:Clonorchiasis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth