The effect of methylprednisolone on monocyte eicosanoid production in patients with multiple sclerosis.
The in vitro effect of methylprednisolone on prostaglandin E2 (PGE2), leukotriene B4 (LTB4) and thromboxane B2 (TXB2) synthesis by adherent monocytes was examined using samples of peripheral blood from 15 patients with multiple sclerosis and 18 normal controls. Eicosanoid production by monocytes was reduced in patients compared with controls and there was a dose-dependent inhibitory effect of methylprednisolone on eicosanoid production in both groups. In vitro production of PGE2 and TXB2 but not LTB4 was reduced in patients with multiple sclerosis following intravenous treatment with methylprednisolone compared with pretreatment samples. In a separate cohort of 20 patients with multiple sclerosis and 15 controls, the in vitro inhibition of PGE2 release by methylprednisolone was not associated with reduced pokeweed-mitogen-stimulated immunoglobulin G synthesis by peripheral blood mononuclear cells. These results suggest that methylprednisolone inhibits monocyte-macrophage function, but this effect is not specific to patients with multiple sclerosis.
Kirk
P F
PF
Department of Medicine, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Williams
J D
JD
Petersen
M M
MM
Compston
D A
DA
D000328:Adult; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D009103:Multiple Sclerosis / Q000276:immunology*; D011043:Pokeweed Mitogens; D011863:Radioimmunoassay; D013929:Thromboxane B2 / Q000096:biosynthesis*
D007074:Immunoglobulin G; D011043:Pokeweed Mitogens; D007975:Leukotriene B4; D013929:Thromboxane B2; D015232:Dinoprostone; D008775:Methylprednisolone