Multiplication of 28S rDNA and NOR activity in chromosome evolution among ants of the Myrmecia pilosula species complex.
Chromosomal localization of rDNA in samples of five taxa of the Mymecia pilosula species complex (Hymenoptera: Formicidae: Myrmeciinae) with 2n = 3 (M. croslandi), 8 (M. imaii), 10 (M. banksi), 18 (M. haskinsorum), and 27 (M. pilosula) was carried out by fluorescence in situ hybridization (FISH) using cloned M. croslandi rDNA (pMc.r2) including the coding region for 28S rRNA. Results show that (1) the 28S rDNA in the genome of these ants is repetitive and is localized in pericentromeric C-bands, (2) the number of chromosomes carrying rDNA is two in M. croslandi, M. imaii and M. banksi, six in M. haskinsorum and ten in M. pilosula, and (3) only one or two clusters of rRNA genes generate nucleoli in each species. We suggest that the rDNA in the ancestral stock of the M. pilosula complex was localized originally in a pericentromeric C-band, and multiplied by chance with time during saltatory increases in C-banding following episodes of centric fission. Most rDNA multiplied on various chromosomes seems to be inactivated and eliminated from the genome, together with C-bands, by AM-inversion or centric fusion, with the remnant rDNAs dispersed in the genome by centric fission and AM-inversion.
Hirai
H
H
Primate Research Institute, Kyoto University, Aichi-ken, Japan.
Yamamoto
M T
MT
Ogura
K
K
Satta
Y
Y
Yamada
M
M
Taylor
R W
RW
Imai
H T
HT
D000818:Animals; D001000:Ants / Q000235:genetics*; D005075:Biological Evolution; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D018628:Gene Dosage; D017344:Genes, Insect / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008957:Models, Genetic; D009697:Nucleolus Organizer Region / Q000235:genetics*; D012339:RNA, Ribosomal, 28S / Q000235:genetics*
D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S