title: DNA double-strand breaks and hairpins in V(D)J recombination.
abstract: Several lines of evidence have suggested the existence of broken DNA molecules as intermediates in V(D)J recombination. These include sequence alterations at the recombined junctions and the presence of aberrant recombinants. More recently, broken DNA molecules have been directly detected in recombinationally active cells. The signal sequence ends have normal blunt-ended DNA breaks, but the coding ends have a hairpin (self-joined) structure that provides an explanation for the self-complementary P nucleotide insertions often found after V(D)J joining in the antigen receptor genes.
authors.affiliation: Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: M
authors.initials: M
authors.lastname: Gellert
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004249:DNA Damage / Q000235:genetics*; D015321:Gene Rearrangement / Q000235:genetics*; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D009690:Nucleic Acid Conformation; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell; D004247:DNA