[Fluorescence monitoring of solid-phase peptide synthesis using a quartz reactor-cuvette].
The quartz reactor-cuvette for fluorescent monitoring of the solid phase peptide synthesis consists of a 10 mm diameter quartz tube jacketed in a 14 x 14 mm orthogonal holder and equipped with chemically inert adapters. This allows to use the reactor with almost any standard fluorimeter. Details of the construction are presented. Fluorescence spectra of Fmoc-group, both in solution and on the solid support, are given; effects of concentration quenching of the fluorescence are studied.
Medvedkin
V N
VN
Permiakov
E A
EA
Uverskiĭ
V N
VN
Gripas'
A F
AF
Mitin
Iu V
IuV
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D010455:Peptides
Fluorestsentnyĭ kontrol' tverdofaznogo sinteza peptidov s primeneniem kvartsevogo reaktora-kiuvety.