Regulation and localization of colony-stimulating factor-1 mRNA in cultured rat dental follicle cells.
Cultured dental follicle cells from rat mandibular molars transcribe colony-stimulating factor-1 (CSF-1) mRNA as determined by reverse-transcription polymerase chain reaction. In turn, the CSF-1 mRNA appears to be translated, as seen by immunoperoxidase staining. Interleukin 1 alpha (IL-1 alpha) stimulates increased transcription of the CSF-1 gene in a concentration- and time-dependent manner. Moreover, CSF-1 itself has an autocrine effect on transcription of the CSF-1 gene. Because others have shown that in vivo injection of CSF-1 accelerates tooth eruption and because the dental follicle is required for eruption to occur, this study demonstrates the possible relation between CSF-1 and the follicle; namely, the source of CSF-1 for tooth eruption might be the dental follicle. In turn, regulation of gene expression for CSF-1 by IL-1 alpha and CSF-1 may play a part in signalling the onset of tooth eruption.
Wise
G E
GE
Department of Veterinary Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
Lin
F
F
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D017931:DNA Primers; D003795:Dental Sac / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000502:physiology; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D008334:Mandible; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014078:Tooth Eruption / Q000235:genetics / Q000502:physiology*
D017931:DNA Primers; D007375:Interleukin-1; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor