title: Identification and sequence analysis of the soxB gene essential for sulfur oxidation of Paracoccus denitrificans GB17.
abstract: The coding region for lithotrophic sulfur oxidation (Sox) in Paracoccus denitrificans GB17 was identified by isolation of a transposon Tn5-mob mutant with a Sox- phenotype (strain TP19). The corresponding wild-type region was cloned previously (G. Mittenhuber, K. Sonomoto, M. Egert, and C. G. Friedrich, J. Bacteriol. 173:7340-7344, 1991). Sequence analysis of a 2.5-kb subclone that complemented strain TP19 revealed that Tn5-mob was inserted into a coding region for a 553-amino-acid polypeptide named SoxB. This polypeptide had an M(r) of 60.573, including a possible signal peptide. The function of the SoxB protein of P. denitrificans GB17 appeared to be identical to that of enzyme B of the thiosulfate-oxidizing enzyme system of Thiobacillus versutus. The amino acid compositions of the two proteins were identical, and the amino acid sequences of three internal peptides of enzyme B as determined by Edman degradation were identical to corresponding sequences of the deduced SoxB protein of P. denitrificans GB17.
authors.affiliation: Lehrstuhl für Technische Mikrobiologie, Fachbereich Chemietechnik, Universität Dortmund, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Wodara
authors.forename: S
authors.initials: S
authors.lastname: Kostka
authors.forename: M
authors.initials: M
authors.lastname: Egert
authors.forename: D P
authors.initials: DP
authors.lastname: Kelly
authors.forename: C G
authors.initials: CG
authors.lastname: Friedrich
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003574:Cytochrome c Group / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010088:Oxidoreductases / Q000235:genetics*; D010230:Paracoccus / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013455:Sulfur / Q000378:metabolism*
chemical_list: D003574:Cytochrome c Group; D013455:Sulfur; D010088:Oxidoreductases; C064500:flavocytochrome c sulfide dehydrogenase; C022746:thiosulfate oxidizing enzyme