Rapid accumulation of fluorescent material with aging in an oxygen-sensitive mutant mev-1 of Caenorhabditis elegans.
Mutations in mev-1 of the nematode Caenorhabditis elegans render animals hypersensitive to high oxygen concentrations. They also reduce life span. To further understand the effects of mev-1 on aging, accumulation of fluorescent material resembling lipofuscin was measured by biochemical and histological analyses. Fluorescent material accumulated in both wild type and mev-1 animals with increasing age. The mev-1 mutant accumulated more fluorescent material at a greater rate than dose wild type. Furthermore, the accumulation rates depended on concentration of oxygen. Since this phenotype has been widely used as an aging marker, these results validate mev-1's use as a model to study aging.
Hosokawa
H
H
Department of Chemistry, Faculty of Science, Tokai University, Kanagawa, Japan.
Ishii
N
N
Ishida
H
H
Ichimori
K
K
Nakazawa
H
H
Suzuki
K
K
D000375:Aging / Q000378:metabolism*; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D004351:Drug Resistance; D005453:Fluorescence; D005470:Fluorometry; D009154:Mutation; D010100:Oxygen / Q000494:pharmacology*; D012016:Reference Values
D010100:Oxygen