Evaluation of free radical production in an ischaemia-reperfusion model in the rabbit using a tourniquet.
Use of a tourniquet on a limb to stem blood flow is frequent in surgery. However, this results in an ischaemia-reperfusion effect that causes damage through the production of free radicals. The aim of this work was to evaluate this production of free radicals in an ischaemia-reperfusion model in the rabbit after placement of a tourniquet. Plasma lipoperoxides were measured by high-performance liquid chromatography using the malondialdehyde assay method. We found significant (P < 0.01) production of free radicals 1 min after release of the tourniquet compared with controls; no variation in levels of free radicals was observed during the ischaemia phase.
Chopineau
J
J
Laboratoire de Pharmacie Clinique et Biotechnique, Faculté de Pharmacie, Clermont-Ferrand, France.
Sommier
M F
MF
Sautou
V
V
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005609:Free Radicals / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008954:Models, Biological; D011817:Rabbits; D015427:Reperfusion Injury / Q000097:blood*; D014111:Tourniquets / Q000009:adverse effects*
D005609:Free Radicals; D008315:Malondialdehyde