title: The MAG1* 3-methyladenine DNA glycosylase gene is closely linked to the SPT15 TATA-binding TFIID gene on chromosome V-R in Saccharomyces cerevisiae.
abstract: The MAG1 gene encodes a 3-methyladenine DNA glycoslyase, which is involved in DNA alkylation repair in Saccharomyces cerevisiae. The mag1 mutant is deficient in 3-methyladenine DNA glycosylase activity and shows enhanced sensitivity to several monofunctional alkylating agents. MAG1 is allelic to MMS5. This gene has been previously located on chromosome V by chromosomal hybridization. We present physical and genetic mapping data here showing that the MAG1 gene is located on chromosome V-R, proximal to and about 10 kilobase pairs away from the SPT15 gene coding for the yeast TATA-binding protein TFIID.
authors.affiliation: Department of Microbiology, University of Saskatchewan, Saskatoon, Canada.
authors.forename: W
authors.initials: W
authors.lastname: Xiao
authors.forename: V
authors.initials: V
authors.lastname: Penugonde
authors.forename: G H
authors.initials: GH
authors.lastname: Rank
mesh_terms: D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D045647:DNA Glycosylases; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli; D008040:Genetic Linkage; D009699:N-Glycosyl Hydrolases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004268:DNA-Binding Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; C021565:3-methyladenine-DNA glycosylase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases