title: Identification of genes differentially expressed in normal lung and non-small cell lung carcinoma tissue.
abstract: Using a magnet-assisted subtraction technique, 17 complementary DNA (cDNA) clones were isolated that were expressed in the normal lung but were decreased or lost in the corresponding tumor tissue of a nonsmall cell lung carcinoma patient. The lack of expression of six magnet-assisted subtraction technique cDNA clones in three additional non-small cell lung carcinoma cases indicates their possible relevance for non-small cell lung carcinoma. Two cDNA clones revealed homology to genes specifically expressed in lung, i.e., pulmonary surfactant-associated protein B and the receptor for advanced glycosylation end products of proteins. Three cDNA clones showed identity to cDNA sequences encoding calmodulin-like protein, glutamine synthetase, and cytoskeletal beta-actin. One cDNA clone is identical to a recently described human expressed sequence tag whose gene is still unknown.
authors.affiliation: Kantonsspital Basel, Zentrum für Lehre und Forschung, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Schraml
authors.forename: R
authors.initials: R
authors.lastname: Shipman
authors.forename: M
authors.initials: M
authors.lastname: Colombi
authors.forename: C U
authors.initials: CU
authors.lastname: Ludwig
mesh_terms: D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification*; D016326:Extracellular Matrix Proteins; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008175:Lung Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D011510:Proteolipids / Q000235:genetics; D011663:Pulmonary Surfactants / Q000235:genetics; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000235:genetics; D013382:Subtraction Technique
chemical_list: D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D011510:Proteolipids; D011663:Pulmonary Surfactants; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; C503126:SPARCL1 protein, human