High-performance liquid chromatographic determination of dietary fibre in raw and processed carrots.
An HPLC method was developed to determine dietary fibre in carrots (Daucus carota L.). Primary hydrolysis of dietary fibre residue was performed with 12 M H2SO4 for 2 h at 40 degrees C and secondary hydrolysis with 0.414 M H2SO4 for 3 h at 100 degrees C. For the neutralization step prior to injection, AG4-X4 resin (Bio-Rad) was used. Neutral monosaccharides were separated using an HPX-87P column (Bio-Rad). This method was applied for evaluation of the quantitative variation of dietary fibre content in carrots during autoclaving.
Redondo
A
A
Departamento de Nutrición y Bromatología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Villanueva
M J
MJ
Rodríguez
M D
MD
D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018552:Daucus carota / Q000737:chemistry*; D004043:Dietary Fiber / Q000032:analysis*; D006868:Hydrolysis; D011134:Polysaccharides / Q000032:analysis; D013464:Sulfuric Acids
D002241:Carbohydrates; D004043:Dietary Fiber; D011134:Polysaccharides; D013464:Sulfuric Acids; C033158:sulfuric acid