[Transport of monovalent cations into erythrocytes of rabbits with experimental hypercholesterolemia: correlation with plasma cholesterol].
The activities of the Na+, K(+)-pump, Na+, K+, 2Cl- and K+, Cl(-)-cotransports and Na+, Li+ exchange as well as intracellular concentrations of Na+, K+, Mg2+ and cholesterol content in erythrocyte membranes of rabbits with experimental hypercholesterolemia have been studied. The activity of the Na+, K(+)-pump recorded as the ouabain-inhibited component of 86Rb influx is, on the average, by 100% higher than that in control erythrocytes of rabbits fed on a cholesterol-rich diet for 2 months and correlates significantly with the concentration of cholesterol (Ch) and low density lipoproteins (LDL) in the plasma as well as with Na+ concentration in erythrocytes. The activity of the Na+, K+, 2Cl- and K+, Cl(-)-cotransports recorded, correspondingly, as the bumetanide- and furosemide-inhibited component of 86Rb influx, is unobserved in rabbit erythrocytes irrespective of the Ch level in the plasma. The activity of the Na+, Li+ exchange is markedly reduced in erythrocytes of rabbits with hypercholesterolemia and correlates with Ch and LDL levels in the plasma. The K+ and Mg2+ concentrations in erythrocytes do not depend on Ch plasma levels. There was a negative correlation between the intracellular Na+ content with plasma Ch and LDL levels. The Ch content in erythrocyte ghosts is, on the average, identical for both groups of experimental animals.
Makarov
V L
VL
Kuznetsov
S R
SR
Churina
S K
SK
Sokolova
A I
AI
D000818:Animals; D001692:Biological Transport; D002414:Cations, Monovalent / Q000097:blood*; D002712:Chlorides / Q000097:blood; D002784:Cholesterol / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006937:Hypercholesterolemia / Q000097:blood*; D008094:Lithium / Q000097:blood; D008274:Magnesium / Q000097:blood; D008297:Male; D011188:Potassium / Q000097:blood; D011817:Rabbits; D012964:Sodium / Q000097:blood
D002414:Cations, Monovalent; D002712:Chlorides; D002784:Cholesterol; D008094:Lithium; D012964:Sodium; D008274:Magnesium; D011188:Potassium
Transport odnovalentnykh kationov v éritrotsitakh krolikov s éksperimental'noĭ giperkholesterinemieĭ: korreliatsii s kholesterinom plazmy.