title: Use of Yarrowia lipolytica hexokinase for the quantitative determination of trehalose 6-phosphate.
abstract: This paper describes a procedure for the quantitative determination of trehalose 6-phosphate (T6P) based on its ability to inhibit hexokinase from Yarrowia lipolytica. The assay is linear between 1 nmol and at least 8 nmol. The concentration of T6P in wild-type Saccharomyces cerevisiae (0.15 mM) and in ras2 mutants (0.25 mM) remained unchanged in the exponential or stationary phase of growth or after heat shock. A tps1 mutant affected in T6P synthase did not show detectable T6P. Heat shock increased the concentration of T6P in Schizosaccharomyces pombe from 0.43 to 0.75 mM.
authors.affiliation: Instituto de Investigaciones Biomédicas C.S.I.C., Madrid, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Blázquez
authors.forename: J M
authors.initials: JM
authors.lastname: Gancedo
authors.forename: C
authors.initials: C
authors.lastname: Gancedo
mesh_terms: D006593:Hexokinase / Q000032:analysis*; D006358:Hot Temperature; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D004718:Saccharomycetales / Q000201:enzymology*; D013403:Sugar Phosphates / Q000032:analysis*; D014199:Trehalose / Q000031:analogs & derivatives* / Q000032:analysis; D015003:Yeasts / Q000201:enzymology*
chemical_list: D013403:Sugar Phosphates; C082722:trehalose-6-phosphate; D014199:Trehalose; D006593:Hexokinase