[Enhanced antibody production by lung lymphocytes after oral immunization with Bordetella pertussis surface antigens].
The purpose of our study was to evaluate the effect of oral vaccination with Bordetella pertussis surface antigens on the immune response at the site of antigen application. We orally immunized female BALB/c mice on five consecutive days and repeated this procedure after a free interval of 10 days. Lymphocytes of the lung (LL), Peyer's patches (PPL) and lamina propria of the gut (LPL) were isolated and the immunoglobulin secretion rate was measured with time-resolved immunofluorescence. Oral immunization was found to enhance the IgA secretion rate by 69.9% in LL compared to unimmunized animals. The IgG synthesis in LL was increased by 28.1% and the IgM synthesis by 14.1%. In addition, an improvement of 47.8% was observed for the IgG secretion in LPL and PPL. Thus, our results demonstrate a strong local immune response after oral immunization with Bordetella pertussis.
Frühwirth
M
M
Kinderklinik, Universität Innsbruck.
Ellemunter
H
H
Ruedl
C
C
Wolf
H
H
D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000008:administration & dosage*; D000954:Antigens, Surface / Q000008:administration & dosage; D001886:Bordetella pertussis / Q000276:immunology*; D005260:Female; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008168:Lung / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Gesteigerte Antikörperproduktion von Lungenlymphozyten nach oraler Immunisierung mit Bordetella-pertussis-Oberflächenantigenen.