[Restoration of transcription antitermination of N- bacteriophages in Escherichia coli with a mutant RNA polymerase].
The N protein of bacteriophage lambda modifies Escherichia coli RNA polymerase in such a way that it transcribes through termination signals, in a process called antitermination. In general N-mutants are not able to perform transcription antitermination. In this paper we report the suppression of N7 and Nmar3 mutations by Escherichia coli ron-lon strain. The lon mutation causes the N protein half-life to raise, suggesting that excess of N7 fragment or Nmar3 protein overcome the defect in antitermination. Under these conditions the lambda N-phages produced a titer similar to lambda wild type, although the plaques were smaller. These observations highlight the relevance of N half life in the regulation of transcription antitermination.
Cisneros
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Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., México, D.F., Mexico.
Vaca
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Sosa
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Montañez
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C
D049069:ATP-Dependent Proteases; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000235:genetics / Q000502:physiology*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D015967:Gene Expression Regulation, Viral; D006207:Half-Life; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D049070:Protease La; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; C059417:N protein, Bacteriophage lambda; D011956:Receptors, Cell Surface; D054334:Viral Regulatory and Accessory Proteins; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases; D012321:DNA-Directed RNA Polymerases; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; C483567:Lon protein, E coli; D049070:Protease La
Restauración de la antiterminación de la transcripción de bacteriófagos N-en Escherichia coli con una RNA polimerasa mutante.