Gene expression of VIP receptor in rat lymphocytes.
In the present report we analyze by reverse transcription (RT) and polymerase chain reaction (PCR) the expression of vasoactive intestinal peptide receptors (VIP-R) in rat thymocytes and peripheral T and B lymphocytes. We have obtained a specific VIP-R cDNA product of 311 bp identical in size to that obtained from lung. These results have been confirmed by Southern hybridization analysis. VIP receptor gene expression in central and peripheral lymphoid organs support the notion that VIP is a immunomodulatory peptide.
Gomariz
R P
RP
Departamento Biologia Celular, Facultad de Biologia, Universidad Complutense, Madrid, Spain.
Garrido
E
E
Leceta
J
J
Martinez
C
C
Abalo
R
R
Delgado
M
M
D000276:Adjuvants, Immunologic; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D001483:Base Sequence; D015139:Blotting, Southern; D017931:DNA Primers; D015870:Gene Expression; D008198:Lymph Nodes / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D051381:Rats; D018005:Receptors, Vasoactive Intestinal Peptide / Q000096:biosynthesis*; D013154:Spleen / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000276:immunology
D000276:Adjuvants, Immunologic; D017931:DNA Primers; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide