Elevated levels of PDGF alpha receptors in keloid fibroblasts contribute to an enhanced response to PDGF.
Despite a number of studies, the etiology of keloids remains unknown. We have investigated the response of fibroblasts derived from keloid tissue and normal adult skin to platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and fibroblast growth factor (FGF). Keloid fibroblasts were more responsive in both chemotactic and mitogenic assays to all three isoforms of PDGF than fibroblasts from normal skin. No enhanced response of the cells to either EGF or FGF was detected. The enhanced PDGF response of keloid fibroblasts appears to be mediated by elevated levels of PDGF alpha receptors, which are 4-5 times higher than those in normal human skin fibroblasts.
Haisa
M
M
Department of Cell Biology and Anatomy, University of Miami School of Medicine, Florida 33136.
Okochi
H
H
Grotendorst
G R
GR
D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D002633:Chemotaxis; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000502:physiology; D005260:Female; D005346:Fibroblast Growth Factors / Q000502:physiology; D005347:Fibroblasts / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007627:Keloid / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000502:physiology*; D017479:Receptors, Platelet-Derived Growth Factor / Q000032:analysis*
D010982:Platelet-Derived Growth Factor; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D004247:DNA; D017479:Receptors, Platelet-Derived Growth Factor