Demonstration of the presence of an interleukin-1 receptor on the surface of murine astrocytes and its regulation by cytokines and Theiler's virus.
Interleukin-1 (IL-1) binding found in rodent brain, and its biological effect on astroglial cells, suggests that the receptor for IL-1 alpha may be located on astrocytes. A radioligand receptor assay performed on pure mouse astrocyte cultures revealed the presence of one binding site [type I, molecular weight (MW) 80,000], with Kd = 0.3 x 10(-1) M), at a density of 150 receptors/cell. The specific, saturable and displaceable high-affinity binding took place in a time- and temperature-dependent way. The receptor-IL-1 alpha complex was quickly endocytosed at 37 degrees but not at 4 degrees. The receptor expression was up-regulated by Theiler's virus, interferon-gamma, tumour necrosis factor and IL-6; was not affected by vaccinia virus infection; and decreased after lipopolysaccharide treatment.
Rubio
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Department of Neuroimmunology, Instituto Cajal, Madrid, Spain.
D000818:Animals; D001253:Astrocytes / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D007375:Interleukin-1 / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D011051:Poliomyelitis / Q000276:immunology*; D017472:Receptors, Interleukin-1 / Q000378:metabolism*; D013045:Species Specificity; D013696:Temperature; D017945:Theilovirus
D016207:Cytokines; D007375:Interleukin-1; D007457:Iodine Radioisotopes; D017472:Receptors, Interleukin-1