Partial characterization and time course analysis of Hymenolepis diminuta coproantigens.
An analysis of Hymenolepis diminuta specific antigens in infected rat faeces was carried out. Using a capture type antibody sandwich ELISA assay based on a hyperimmune rabbit anti-worm somatic antisera it was demonstrated that, although antigen was present in faeces before patency, the onset of egg production led to a sharp increase in the levels of parasite antigen in the faeces. Levels of antigen in host faeces were independent of worm burden. Parasite eggs did not contribute significantly to faecal antigen levels. Western blot analysis indicated a number of highly specific antigens at around M(r) 69,000, M(r) 37,000, M(r) 50,000 and M(r) 27,000 with a low molecular weight smear at between M(r) 30,000 and M(r) 34,000 present in the faeces of H. diminuta infected rats. Some cross reaction occurred with an antigen of around M(r) 66,000 in the faeces of non H. diminuta infected rodents. Antibody activity against this antigen was removed by affinity adsorption of the antibody solution against normal rat faeces.
Allan
J C
JC
Department of Biological Sciences, University of Salford, UK.
Craig
P S
PS
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D006926:Hymenolepis / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D008970:Molecular Weight; D010270:Parasite Egg Count; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D000947:Antigens, Helminth; D007106:Immune Sera