[The effect of leukotrienes B4, C4, 12-hydroxyeicosatetraenoic acid and of the threo- and erythro-epimers of hepoxilin B3 on the plasticity of the neuronal cholinoreceptors in the snail].
Effects of several acyclic eicosanoids (which are formed from arachidonic acid under the action of 5- and 12-lipoxygenases) on desensitization of somatic cholinoreceptors of Helix lucorum RPa3 and LPa3 neurons were studied by means of two-electrode voltage clamp technique. The substances under study were: leukotrienes B4, C4, 12(S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoic acid (12-HETE), threo- and erythro-epimers of hepoxilin B3. Cholinoreceptor plasticity was evaluated by a degree of depression of inward current, induced by rhythmical local ACh applications on the soma with intervals 55-180 sec. Leukotrienes B4 and C4 intensified cholinoreceptor plasticity. Inhibitor of inositol-1,4,5-trisphosphate-dependent (IP3-d) mobilization of deposited Ca2+, TMB-8, prevented the effect of leukotriene C4. 12-HETE, threo- and erythro-epimers of hepoxilin B3 did not change plasticity. A conclusion is made that cholinoreceptor plasticity is controlled by leukotrienes B4 and C4. One of the regulatory pathways includes IP3-d Ca-mobilization. Eicosanoids from 12-lipoxygenase pathway (12-HETE, threo- and erythro-epimers of hepoxilin B3) do not participate in this intracellular regulatory mechanism.
Pivovarov
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D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D006372:Helix, Snails; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D066298:In Vitro Techniques; D007536:Isomerism; D007975:Leukotriene B4 / Q000494:pharmacology*; D017997:Leukotriene C4 / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009473:Neuronal Plasticity / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D011950:Receptors, Cholinergic / Q000187:drug effects* / Q000502:physiology
D006893:Hydroxyeicosatetraenoic Acids; D011950:Receptors, Cholinergic; D007975:Leukotriene B4; D017997:Leukotriene C4; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C022948:10-hydroxy-11,12-epoxyeicosa-5,8,14-trienoic acid; D015126:8,11,14-Eicosatrienoic Acid
Vliianie leĭkotrienov B4, C4, 12-gidroksiéĭkozatetraenovoĭ kisloty, treo- i éritro-épimerov gepoksilina B3 na plastichnost' kholinoretseptorov neĭronov vinogradnoĭ ulitki.