The mitogen-induced generation of interferon-gamma producing cells in cultures of rhesus monkey peripheral blood mononuclear cells is age-dependent.
An enzyme-linked immunoabsorbent spot (ELISPOT) assay has been developed for measuring the frequency of interferon-gamma (IFN-gamma) producing cells in rhesus monkeys. Aged monkeys revealed, upon mitogenic stimulation, a significantly higher percentage of IFN-gamma secreting peripheral blood mononuclear cells (PBMC) compared to young animals. No correlation was found between the frequency of IFN-gamma producing PBMC and the mitogen-driven proliferation, indicating that in rhesus monkeys no direct correlation exists between these two activation parameters.
van Besouw
N M
NM
Department of Chronic and Infectious Diseases, Institute for Applied Radiobiology and Immunology (ITRI)-TNO, Rijswijk, The Netherlands.
van der Meide
P H
PH
Bakker
N P
NP
D000367:Age Factors; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008213:Lymphocyte Activation; D008253:Macaca mulatta / Q000276:immunology*; D008297:Male; D012737:Sex Factors; D018709:Statistics, Nonparametric; D013268:Stimulation, Chemical
D003208:Concanavalin A; D007371:Interferon-gamma