title: The rate and source of albumin entry into saline-filled experimental retinal detachments.
abstract: OBJECTIVE
  To investigate the rate and source of albumin entry into experimental nonrhegmatogenous detachments.
  
  
  METHODS
  Detachments were made in Dutch rabbits by injecting Hanks' balanced salt solution into the subretinal space through a micropipette. Subretinal fluid was withdrawn 0 to 4 hours later through a similar micropipette and analyzed for osmolality and albumin content (by gel electrophoresis). Sodium iodate was injected intravenously in some rabbits to damage the retinal pigment epithelium (RPE). In some rabbits fluorescein isothiocyanate albumin (FITC-albumin) was injected intravitreally or intravenously to measure its entry into the subretinal fluid by fluorophotometry. Results from 4 to 8 eyes were averaged for each data point.
  
  
  RESULTS
  The albumin concentration and total amount of albumin in the subretinal fluid increased steadily over 4 hours in retinal detachments initially filled with Hanks' solution. Pretreating rabbits with sodium iodate injection resulted in a 50-fold increase in the rate of albumin entry, although the levels were still low relative to those of serum. Intravitreal FITC-albumin entered the subretinal fluid at a rate independent of sodium iodate damage, but intravenous FITC-albumin only entered the subretinal space after RPE damage. Subretinal fluid osmolality remained within the range of 291 to 294 mOsm/kg, irrespective of sodium iodate damage or differences in the rate of fluid absorption.
  
  
  CONCLUSIONS
  These results indicate that albumin can diffuse into the rabbit subretinal space from both vitreous and bloodstream, although entry from serum requires damage to the RPE. Subretinal fluid appears to be transported actively (control eyes) or passively (iodate-damaged eyes) out of the subretinal space, despite albumin entry and without major osmolar shifts.
authors.affiliation: Department of Ophthalmology, Beckman Center, Stanford University Medical Center, California.
authors.forename: A
authors.initials: A
authors.lastname: Takeuchi
authors.forename: G
authors.initials: G
authors.lastname: Kricorian
authors.forename: X Y
authors.initials: XY
authors.lastname: Yao
authors.forename: J W
authors.initials: JW
authors.lastname: Kenny
authors.forename: M F
authors.initials: MF
authors.lastname: Marmor
mesh_terms: D000818:Animals; D001813:Blood-Retinal Barrier; D001826:Body Fluids; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism; D015590:Fluorophotometry; D007452:Iodates / Q000494:pharmacology; D009994:Osmolar Concentration; D010857:Pigment Epithelium of Eye / Q000187:drug effects; D011817:Rabbits; D012163:Retinal Detachment / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012965:Sodium Chloride
chemical_list: D007452:Iodates; D012709:Serum Albumin; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D012965:Sodium Chloride; D016650:Fluorescein-5-isothiocyanate; C032285:sodium iodate