A single mycobacterial protein (hsp 65) expressed by a transgenic antigen-presenting cell vaccinates mice against tuberculosis.
We used a retroviral shuttle vector [pZIPNeoSV(X)] to transfect a monocyte-like murine tumour cell line (J774.G8) with the Mycobacterium leprae gene encoding heat-shock protein (hsp) 65. The antigen was expressed and presented on the surface of the transfected cell in association with major histocompatibility complex (MHC) class I and class II for recognition by T cells from specifically sensitized mice. We show here that when these transfected cells were used as a vaccine and introduced parentally into syngeneic (BALB/c) mice they conferred a remarkably high degree of protective immunity against subsequent challenge with either M. bovis bacillus Calmette-Guérin (BCG) or M. tuberculosis H37Rv.
Silva
C L
CL
Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paolo, Brazil.
Lowrie
D B
DB
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001426:Bacterial Proteins; D001428:Bacterial Vaccines / Q000276:immunology*; D018834:Chaperonin 60; D018833:Chaperonins / Q000276:immunology*; D005260:Female; D006360:Heat-Shock Proteins / Q000276:immunology*; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009166:Mycobacterium leprae / Q000235:genetics / Q000276:immunology*; D014162:Transfection; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control*
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins