A rapid multiresidue screen for organophosphorus, organochlorine, and N-methyl carbamate insecticides in plant and animal tissues.
A multiresidue screen for the quantitative determination of 43 organophosphorus, 17 organochlorine, and 11 N-methyl carbamate insecticides in 10 g of plant or animal tissues is described. The insecticides are extracted with 5% ethanol in ethyl acetate (v/v). Samples with high lipid content are cleaned up by automated gel permeation chromatography with a 30% ethyl acetate in hexane (v/v) eluant and in-line silica gel minicolumns. Highly pigmented samples are cleaned up with class-specific solid-phase extraction columns. The concentrated extracts are analyzed by selective detection with gas chromatography or liquid chromatography. Recovery of 71 insecticides ranged from 77 to 113%. Analysis of fortified bovine liver (n = 5) resulted in an average recovery of 96 +/- 4% at the 0.5 to 0.05 micrograms/g level. Analysis of fortified alfalfa hay (n = 5) resulted in a mean recovery of 94 +/- 4% at the 0.06 to 0.5 micrograms/g level, and analysis of fortified fresh tomatoes (n = 5) resulted in an average recovery of 97 +/- 3% at the 0.06 to 0.5 micrograms/g level. Method detection limits ranged from 0.02 to 0.5 micrograms/g for the compounds studied with a nominal 10 g sample.
Holstege
D M
DM
University of California, California Veterinary Diagnostic Laboratory System, Davis 95616.
Scharberg
D L
DL
Tor
E R
ER
Hart
L C
LC
Galey
F D
FD
D000085:Acetates; D000818:Animals; D002219:Carbamates; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods / Q000706:statistics & numerical data; D002853:Chromatography, Liquid / Q000379:methods / Q000706:statistics & numerical data; D005504:Food Analysis / Q000379:methods* / Q000706:statistics & numerical data; D005506:Food Contamination; D007306:Insecticides / Q000032:analysis*; D008099:Liver / Q000737:chemistry; D018551:Solanum lycopersicum / Q000737:chemistry; D000455:Medicago sativa / Q000737:chemistry; D009943:Organophosphorus Compounds; D010573:Pesticide Residues / Q000032:analysis*; D012680:Sensitivity and Specificity; D012997:Solvents
D000085:Acetates; D002219:Carbamates; D007306:Insecticides; D009943:Organophosphorus Compounds; D010573:Pesticide Residues; D012997:Solvents; C007650:ethyl acetate