Cloning of cDNAs and the molecular evolution of a bovine MHC class II DRA gene.
Two overlapping cDNA clones coding for bovine major histocompatibility complex (MHC) class II antigen DRA chain were isolated and characterized. The full-length cDNA clone, MR1, encoded a primary translated product of 253 amino acids, 24 of which were deduced to be a signal peptide and 229 which formed a mature polypeptide. The amino acid sequences deduced from this clone resembled those of class II A molecules from other species in both size and structure, but no potential consensus site of N-linked glycosylation comparable to those in the human, mouse, rat and swine proteins was found in the alpha 2 domain, as well as ovine and equine DRA molecules. Comparison of amino acid sequences encoded by class II A genes among several species and a dendrogram constructed from these data places the DRA gene and the DQA/DYA genes on two distinct branches of a phylogenetic tree, with bovine DRA and ovine DRA being most similar on the DRA branch.
Aida
Y
Y
Laboratory of Gene Technology and Safety, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Kohda
C
C
Morooka
A
A
Nakai
Y
Y
Ogimoto
K
K
Urao
T
T
Asahina
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002417:Cattle / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D015723:Gene Library; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens / Q000235:genetics*; D059805:HLA-DR alpha-Chains; D006801:Humans; D008221:Lymphoid Tissue / Q000276:immunology; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D006684:HLA-DR Antigens; D059805:HLA-DR alpha-Chains