Transgenic X. laevis embryos from eggs transplanted with nuclei of transfected cultured cells.
Transgenic Xenopus laevis embryos were produced by transplantation of transfected cultured cell nuclei into unfertilized eggs. A Xenopus cell line, X-C, was stably transfected with plasmids containing a hygromycin-resistance gene and genes for either beta-galactosidase with a heat shock promoter or chloramphenicol acetyltransferase (CAT) with a muscle-specific actin promoter. Nuclei transplanted from these cells into unfertilized eggs directed development of embryos containing stably integrated copies of the plasmids in each cell. Transgenic embryos showed somite-specific expression of CAT and uniform expression of beta-galactosidase. Transgenic embryos produced by nuclear transplantation should be useful for testing the function of cloned genes in amphibian development.
Kroll
K L
KL
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Gerhart
J C
JC
D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000502:physiology; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002934:Cinnamates; D004351:Drug Resistance; D004625:Embryo, Nonmammalian / Q000502:physiology*; D015870:Gene Expression; D017930:Genes, Reporter; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D053652:Nuclear Transfer Techniques; D010063:Ovum / Q000502:physiology; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014162:Transfection; D014982:Xenopus laevis; D001616:beta-Galactosidase / Q000235:genetics
D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase