title: Apoptosis, but not necrosis, of infected monocytes is coupled with killing of intracellular bacillus Calmette-Guérin.
abstract: We have examined the effect of killing of host monocytes infected with bacillus Calmette-Guérin (BCG) on the viability of the intracellular mycobacteria. Peripheral blood monocytes were infected in vitro with a single bacillus per cell and maintained in culture for 6-8 d to allow the bacilli to replicate. Replicating viable BCG were found singly in perinuclear vacuoles bounded by tightly apposed lipid bilayers. Monocytes were then exposed to toxic mediators that induced killing of cells as evaluated by 51Cr release into the culture medium. Both hydrogen peroxide (H2O2) (an inducer of cell necrosis) and adenosine triphosphate (ATP4-) (an inducer of cell apoptosis) treatment killed infected monocytes. H2O2-induced killing had no effect on BCG viability. ATP-induced cell death was accompanied by DNA fragmentation and nuclear condensation. Apoptosis was associated with a swelling of the phagocytic vacuoles which became multibacillary and with a reduction of BCG viability as enumerated by colony-forming units.
authors.affiliation: Department of Cellular Physiology and Immunology, Rockefeller University, New York 10021.
authors.forename: A
authors.initials: A
authors.lastname: Molloy
authors.forename: P
authors.initials: P
authors.lastname: Laochumroonvorapong
authors.forename: G
authors.initials: G
authors.lastname: Kaplan
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D017209:Apoptosis; D001770:Blood Bactericidal Activity; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D009163:Mycobacterium bovis / Q000276:immunology* / Q000648:ultrastructure; D009336:Necrosis; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D004247:DNA; D006861:Hydrogen Peroxide