title: Transient localized accumulation of actin in Caenorhabditis elegans blastomeres with oriented asymmetric divisions.
abstract: During Caenorhabditis elegans embryogenesis, specific cells in the P1 lineage rotate their duplicated centrosome pair onto the anterior-posterior axis; this rotation is correlated with and necessary for a differential inheritance of cytoplasmic determinants in the daughter cells. Centrosome pair rotation is sensitive to inhibitors of actin and microtubule polymerization and may require microtubule attachment to a specific cortical site. We show that actin and the barbed-end binding protein, capping protein, transiently accumulate at this cortical site, possibly by assembly onto persistent remnants of previous cell divisions. Based on these observations, we propose a model for the molecular basis of centrosome rotation that is consistent with the dependence of rotation on actin filaments and microtubules.
authors.affiliation: Department of Genetics, Washington University School of Medicine, St Louis, MO 63110.
authors.forename: J A
authors.initials: JA
authors.lastname: Waddle
authors.forename: J A
authors.initials: JA
authors.lastname: Cooper
authors.forename: R H
authors.initials: RH
authors.lastname: Waterston
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001757:Blastomeres / Q000378:metabolism*; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D002503:Centromere / Q000502:physiology; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000502:physiology; D009024:Morphogenesis / Q000502:physiology; D009154:Mutation / Q000502:physiology
chemical_list: D000199:Actins