title: Estimation of monoamine oxidase concentrations in soluble and membrane-bound preparations by inhibitor binding.
abstract: A modification of the [3H]-pargyline labelling technique is presented for determining the active-site concentration of monoamine oxidase in soluble preparations. Kinetic considerations show that the rate of reaction of MAO-A with low concentrations of free pargyline will be very much slower than that of MAO-B. Failure to use adequate reaction times for the concentration of pargyline added can lead to gross underestimation of the quantity of MAO-A present.
authors.affiliation: Department of Biochemistry, Trinity College, Dublin, Ireland.
authors.forename: M C
authors.initials: MC
authors.lastname: Anderson
authors.forename: K F
authors.initials: KF
authors.lastname: Tipton
mesh_terms: D000818:Animals; D002417:Cattle; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000378:metabolism*; D009994:Osmolar Concentration; D010293:Pargyline / Q000378:metabolism; D012995:Solubility; D013997:Time Factors
chemical_list: D010293:Pargyline; D008995:Monoamine Oxidase