Lactate oxidation coupled to energy production in mitochondria like particles from Setaria digitata, a filarial parasite.
In the filarial parasite, Setaria digitata, the mitochondria like particles (MLP) show NAD reduction with sodium lactate. The MLP also reduces dye and ferricyanide with lactate. The ferricyanide reduction by lactate is found to be sensitive to the cytochrome o inhibitor orthohydroxy diphenyl (OHD) and complex I inhibitor rotenone, modulated by ADP (+) and ATP (-) and inhibited by pyruvate and oxaloacetate. MLP shows lactate oxidation sensitive to OHD, rotenone and sodium malonate. Thus, the lactate utilizing complex system, consisting of an NADH generating MLP bound lactate dehydrogenase and a lactate flavocytochrome reductase tightly linked to complex I and cytochrome o, produces ATP in functional association with fumarate reductase complex and other enzyme systems. Hence, this study provides new dimensions to the study of metabolism in filarial parasites.
Sivan
V M
VM
Department of Biochemistry, University of Kerala, Thiruvananthapuram, India.
Raj
R K
RK
D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology; D005292:Ferricyanides; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D042844:L-Lactate Dehydrogenase (Cytochrome); D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D008314:Malonates / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D015388:Organelles / Q000187:drug effects / Q000378:metabolism*; D010071:Oxaloacetates / Q000494:pharmacology; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D011773:Pyruvates / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D017182:Setaria Nematode / Q000378:metabolism*
D001713:Biphenyl Compounds; D005292:Ferricyanides; D007773:Lactates; D008314:Malonates; D010071:Oxaloacetates; D011773:Pyruvates; D012402:Rotenone; D009243:NAD; C007931:hexacyanoferrate III; D019344:Lactic Acid; C004369:2-phenylphenol; D007770:L-Lactate Dehydrogenase; D042844:L-Lactate Dehydrogenase (Cytochrome)