title: 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification.
authors.affiliation: QIAGEN Inc., Chatsworth, CA.
authors.forename: J
authors.initials: J
authors.lastname: Crowe
authors.forename: H
authors.initials: H
authors.lastname: Döbeli
authors.forename: R
authors.initials: R
authors.lastname: Gentz
authors.forename: E
authors.initials: E
authors.lastname: Hochuli
authors.forename: D
authors.initials: D
authors.lastname: Stüber
authors.forename: K
authors.initials: K
authors.lastname: Henco
mesh_terms: D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006639:Histidine; D009532:Nickel / Q000378:metabolism; D009571:Nitrilotriacetic Acid; D009875:Operator Regions, Genetic; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification*
chemical_list: D002614:Chelating Agents; D011993:Recombinant Fusion Proteins; D006639:Histidine; D009532:Nickel; D009571:Nitrilotriacetic Acid