title: Crystallization and preliminary X-ray analysis of the Fab fragment of a human monoclonal IgM rheumatoid factor (2A2).
abstract: Crystals of the Fab fragment of a human monoclonal IgM rheumatoid factor have been obtained and are suitable for X-ray structure determination. This molecule, derived from the synovial B cells of a patient with rheumatoid arthritis, is an autoantibody with specificity for IgG Fc. The crystals have space group P2(1), cell dimensions a = 69.0 A, b = 76.6 A, c = 98.8 A and beta = 90.6 degrees, and diffract to a resolution of at least 2.8 A.
authors.affiliation: Randall Institute, King's College London, U.K.
authors.forename: M K
authors.initials: MK
authors.lastname: Sohi
authors.forename: B J
authors.initials: BJ
authors.lastname: Sutton
authors.forename: A L
authors.initials: AL
authors.lastname: Corper
authors.forename: T
authors.initials: T
authors.lastname: Wan
authors.forename: R N
authors.initials: RN
authors.lastname: Maini
authors.forename: C
authors.initials: C
authors.lastname: Brown
authors.forename: T
authors.initials: T
authors.lastname: Rijnders
authors.forename: D
authors.initials: D
authors.lastname: Beale
authors.forename: A
authors.initials: A
authors.lastname: Feinstein
authors.forename: A S
authors.initials: AS
authors.lastname: Humphreys
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007075:Immunoglobulin M / Q000737:chemistry*; D012217:Rheumatoid Factor / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D012217:Rheumatoid Factor