Egress route of emulsified 20 centistokes silicone oil from anterior chamber of rabbit.
Silicone oil is used in recent clinical practice, however, it may cause adverse reactions in the eyes. When the high viscosity silicone oil is contaminated with low molecular weight silicone oil, the contamination may cause ocular toxicity or elevation of the intraocular pressure. To obtain information on the distribution of this preparation, emulsified 20 centistokes silicone oil was injected into the anterior chamber of rabbit eyes. The silicone oil droplets were visualized by light and electron microscopy by using oil soluble phthalocyanine blue. This copper containing dye remains in the tissue after removal of the silicone oil by organic solvents. Two and 4 weeks after an injection, the silicone emulsion was observed as numerous small vacuoles with blue precipitate at the margin of vacuoles within elongated trabecular endothelial cells, fibroblasts along the route of uveoscleral outflow and cells of the iris. Three hours after the injection, only a few vacuoles were present in these cells. These results demonstrated that the emulsified silicone oil leaves the anterior chamber through the conventional and unconventional routes. Phagocytosis by the trabecular endothelial cells and fibroblasts along the uveoscleral route caused an accumulation of the emulsified silicone oil in these cells. With chronic exposure to emulsified silicone oil, changes in the trabecular meshwork may lead to a reduction in the outflow of aqueous humor and cause glaucoma.
Ohira
A
A
Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
Chihara
E
E
Soji
T
T
D000818:Animals; D000867:Anterior Chamber / Q000378:metabolism* / Q000648:ultrastructure; D002463:Cell Membrane Permeability; D004655:Emulsions; D007202:Indicators and Reagents; D007211:Indoles; D007498:Iris / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D009942:Organometallic Compounds; D011817:Rabbits; D012827:Silicone Oils / Q000493:pharmacokinetics*; D014617:Vacuoles / Q000648:ultrastructure; D014783:Viscosity
D004655:Emulsions; D007202:Indicators and Reagents; D007211:Indoles; D009942:Organometallic Compounds; D012827:Silicone Oils; C015445:copper phthalocyanine