Plant activating sequences: positively charged peptides are functional as transcriptional activation domains.
Plant sequences that act as transcriptional activation domains in yeast as well as in plants have been isolated by genetic selection in yeast. The selection was based on the reconstitution of a functional GAL4 transcriptional activator. Since the peptides show no homology with reported activation domains, they represent a new class of activating sequences. The sequence P1, which is 10 amino acids long, is the shortest functional activation domain reported. A cDNA that encodes the P14 class (peptides P14-P18) activating sequence have been cloned. The protein exhibits strong homology (higher than 50% amino acid identity) with the BBC1-related sequences, a highly conserved family of basic proteins containing nuclear localization signals. The P14 and P15 peptides are the most effective plant activating sequences. The P14 and P15 peptides are highly hydrophilic, positively charged and mostly unstructured. These properties are at odds with the ones usually found in known activation domains.
Estruch
J J
JJ
CIBA Agricultural Biotechnology, Research Triangle Park, NC 27709-2257.
Crossland
L
L
Goff
S A
SA
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014026:Nicotiana / Q000737:chemistry* / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D018076:DNA, Complementary; D004271:DNA, Fungal; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors