Hydroxystearic acid effects on CDC2/histone H1 kinase activity in C108 carcinoma cells.
HSA at appropriate concentrations shows cytostatic and/or cytotoxic effects on murine Lewis carcinoma cell line C108. The cytostatic effect is mediated by an arrest in the cell cycle machinery, with accumulation of cells in G2-M. The combination of enzymatic assays, cell cycle kinetics studies and immunoprecipitation shows that HSA causes to a certainty an accumulation of cells in the M phase, while a similar effect in G2 has still to be demonstrated. It also inhibits histone H1 kinase activity up to 95% of that of mitotic cells, having as a direct or indirect target the cdc2 complex.
Casali
E
E
Institute of Biological Chemistry, University of Parma, Italy.
Gesmundo
N
N
Farruggia
G
G
Spisni
A
A
Masotti
L
L
D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D018827:Carcinoma, Lewis Lung / Q000201:enzymology* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D015739:Nocodazole / Q000494:pharmacology; D006656:Protamine Kinase / Q000037:antagonists & inhibitors*; D013229:Stearic Acids / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D013229:Stearic Acids; C070375:9-hydroxystearic acid; C070376:10-hydroxystearic acid; D006656:Protamine Kinase; D016203:CDC2 Protein Kinase; D015739:Nocodazole; D006918:Hydroxyurea