Effect of anordiol on ovarian hormone secretion, ovulation, and uterine and vaginal responses in the immature rat.
The purpose of this investigation was to determine the estrogenic and antiestrogenic activities of the potential contraceptive agent anordiol in the immature rat. A dose of 2.45 mumol of anordiol (AD), estradiol (E2), clomiphene citrate (CC), tamoxifen (TA), or the vehicle alone was administered to rats on the 25th and 29th days of age. Serum hormones were measured between 16:00 and 17:00 on days 30, 31, and 32; organ weights were determined on day 32. Anordiol and estradiol treatments significantly increased ovarian and uterine weight and serum LH concentrations, but CC and TA had no effect on these parameters. Vaginal cornification occurred before day 32 in all rats receiving anordiol or estradiol and in 3/5 and 4/5 rats receiving CC and TA, respectively, but not in control rats. Based on serum progesterone levels, ovulation was induced only in rats receiving anordiol or estradiol. All of the compounds tested significantly increased serum testosterone above levels in control animals, but both AD and E2 induced ovulation without a further increase in serum testosterone. We conclude that in the immature rat anordiol produces estrogenic responses in the vagina, uterus and in the hypothalamic-pituitary-ovarian axis, whereas TA and CC are estrogenic only on the vaginal and uterine epithelium.
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Department of Obstetrics and Gynecology, Northwestern University Medical School, Chicago, Illinois 60611.
Chatterton
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RT
D000818:Animals; D002996:Clomiphene / Q000494:pharmacology; D003281:Contraceptives, Postcoital / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005836:Genitalia, Female / Q000187:drug effects*; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D009635:Norandrostanes / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects*; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D013629:Tamoxifen / Q000494:pharmacology; D014599:Uterus / Q000187:drug effects; D014621:Vagina / Q000187:drug effects
D003281:Contraceptives, Postcoital; D012739:Gonadal Steroid Hormones; D009635:Norandrostanes; D013629:Tamoxifen; D002996:Clomiphene; D004958:Estradiol; C023615:anordiol