title: The effect of low temperature on the enzyme activities and the level of SH groups in benign gastric ulcer and gastric carcinoma.
abstract: Homogenates of human benign gastric ulcers and gastric carcinomas were stored at a low temperature (4 degrees C). The activities of L-lactate dehydrogenase (LDH), malic dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PDH), glutathione reductase (GR) and the concentrations of SH-containing compounds showed changes. Increased activities of LDH, MDH, G6PDH and GR occurred in the gastric carcinomas stored at 4 degrees C compared with controls at 37 degrees C. In contrast, the activities of G6PDH and GR decreased in the tissues of benign gastric ulcers. On incubation at 4 degrees C the SH-containing compounds incorporated into protein molecules, SH groups binding with protein and non-protein SH groups decreased in both the benign and malignant tissues.
authors.affiliation: Department of Cell Biology, M. Skłodowska-Curie University, Lublin, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Rzymowska
mesh_terms: D013437:Carbon-Sulfur Lyases / Q000378:metabolism*; D003080:Cold Temperature; D005980:Glutathione Reductase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D010088:Oxidoreductases / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000201:enzymology*; D013276:Stomach Ulcer / Q000201:enzymology*
chemical_list: D010088:Oxidoreductases; D005980:Glutathione Reductase; D013437:Carbon-Sulfur Lyases