title: Isolation and 1H-NMR spectroscopic identification of poly(3-hydroxybutanoate) from prokaryotic and eukaryotic organisms. Determination of the absolute configuration (R) of the monomeric unit 3-hydroxybutanoic acid from Escherichia coli and spinach.
abstract: Trace amounts of poly[(R)-3-hydroxybutanoate] were isolated from competent Escherichia coli, spinach, bovine serum albumin, beef heart mitochondria, and aortal tissues, all sources in which it is not accumulated as storage material. Its identity was in all cases proved by 1H-NMR spectroscopy. In some runs, the poly[(R)-3-hydroxybutanoate] isolated from competent E. coli also contained some 3-hydroxyvalerate, an observation confirmed by 1H-NMR spectroscopy and gas chromatography. The absolute configuration of the polymers isolated from E. coli and spinach was shown to be (all-R) by gas chromatography on chiral columns.
authors.affiliation: Laboratorium für Organische Chemie der Eidgenössischen Technischen Hochschule, Zürich, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Seebach
authors.forename: A
authors.initials: A
authors.lastname: Brunner
authors.forename: H M
authors.initials: HM
authors.lastname: Bürger
authors.forename: J
authors.initials: J
authors.lastname: Schneider
authors.forename: R N
authors.initials: RN
authors.lastname: Reusch
mesh_terms: D000818:Animals; D001011:Aorta / Q000737:chemistry; D002417:Cattle; D002849:Chromatography, Gas; D004926:Escherichia coli / Q000737:chemistry*; D005612:Freeze Drying; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008929:Mitochondria, Heart / Q000737:chemistry; D008968:Molecular Conformation; D015394:Molecular Structure; D009131:Muscle, Smooth, Vascular / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry* / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000737:chemistry; D018724:Spinacia oleracea / Q000737:chemistry*; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D011091:Polyesters; D012710:Serum Albumin, Bovine; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates