Dual pathways regulate neurite outgrowth in enteric ganglia.
Primary cultures of guinea pig myenteric plexus ganglia were used to examine the ability of agents that activate adenylate cyclase or mimic intracellular adenosine 3',5'-cyclic monophosphate (cAMP) to stimulate morphological growth. Dose-dependent increases in neurite length and density were produced in enteric neuronal cultures by forskolin (212% of control), cholera toxin (356% of control), or the permeant cAMP analogues 8-bromoadenosine 3',5'-cyclic monophosphate and dibutyryl cAMP. (R)-p-adenosine 3',5'-cyclic monophosphorothioate, an inhibitor of cAMP-dependent kinases, blocked the growth-promoting effects of cAMP analogues but not of nerve growth factor (NGF). Activation of cAMP-dependent signaling pathways also increased production of mRNA for alpha-tubulin and microtubule-associated protein 2. Dual pathways, regulated by NGF and cAMP-dependent protein kinases, influence growth signaling in enteric ganglia.
Simeone
D M
DM
Department of Surgery, University of Michigan, Ann Arbor 48109-0331.
Romanchuk
G
G
Mulholland
M W
MW
D000818:Animals; D001483:Base Sequence; D000242:Cyclic AMP / Q000378:metabolism; D005724:Ganglia / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006168:Guinea Pigs; D007422:Intestines / Q000294:innervation*; D007425:Intracellular Membranes / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism; D016501:Neurites / Q000502:physiology*; D015345:Oligonucleotide Probes / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D013268:Stimulation, Chemical; D014404:Tubulin / Q000235:genetics
D008869:Microtubule-Associated Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014404:Tubulin; D000242:Cyclic AMP