Development of an ELISA assay for Clostridium perfringens phospholipase C (alpha toxin).
A new method for the assay of Clostridium perfringens alpha toxin (phospholipase C) is described using a sandwich ELISA. This assay has been shown to be quantitative, to have a high specificity for the toxin and is capable of detecting purified Clostridium perfringens phospholipase C at concentrations of as little as 0.005 units/ml in cooked meat culture medium.
Holdsworth
R J
RJ
Department of Surgery, Ninewells Hospital and Medical School, Dundee, United Kingdom.
Parratt
D
D
D000818:Animals; D000992:Antitoxins / Q000276:immunology; D003016:Clostridium perfringens / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006736:Horses; D012680:Sensitivity and Specificity; D010738:Type C Phospholipases / Q000032:analysis* / Q000276:immunology
D000992:Antitoxins; D010738:Type C Phospholipases