Cloning and analysis of a FLO5 flocculation gene from S. cerevisiae.
A yeast flocculation gene was isolated from a genomic library of an FLO5 strain of S. cerevisiae on the basis of its ability to trigger flocculation in a non-flocculent strain. Characterization of the cloned gene by restriction mapping, Southern analysis, and chromosome mapping have shown that it corresponds to a FLO5 gene previously located on chromosome I and that this gene is related to the already described FLO1 gene. A study of gene expression in different yeast strains has indicated that, while this gene is dominant, its expression can be suppressed in some genetic backgrounds. A Northern-blot analysis has demonstrated that the same 5000-nt transcript was present in an FLO5 and an FLO1 strain. A gene disruption experiment has led to the conclusion that another flocculation gene is present and can be active in the FLO5 strain we used.
Bidard
F
F
Laboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne (I.P.V.), INRA, Montpellier, France.
Blondin
B
B
Dequin
S
S
Vezinhet
F
F
Barre
P
P
D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D004271:DNA, Fungal