title: Separation and characterization of rice proteins.
abstract: Rice proteins from nine tissues and one organelle (leaf, chloroplast, stem, root, germ, dark germinated seedling, seed, bran, chaff and callus) were isolated and then separated by two-dimensional gel electrophoresis (2-DE). The protein spots were characterized according to molecular weight, isoelectric point and partial amino-terminal sequence. Electrophoresis was carried out by isoelectric focusing (IEF), nonequilibrium pH gradient electrophoresis (NEPHGE) and immobilized pH gradient (IPG) in the first dimension, and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension. With the aid of nine marker proteins, the patterns of IEF, NEPHGE and IPG 2-DE gels were graphically combined by computer into a single synthetic image for each tissue, respectively, and these images for the nine tissues and one organelle were again combined into a single 2-DE image for the integrated rice protein spots. The rice 2-DE gel image resolved 4892 proteins. About 3% of the spots are characterized by amino-terminal sequencing.
authors.affiliation: Research Institute for Biosciences, Science University of Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tsugita
authors.forename: T
authors.initials: T
authors.lastname: Kawakami
authors.forename: Y
authors.initials: Y
authors.lastname: Uchiyama
authors.forename: M
authors.initials: M
authors.lastname: Kamo
authors.forename: N
authors.initials: N
authors.lastname: Miyatake
authors.forename: Y
authors.initials: Y
authors.lastname: Nozu
mesh_terms: D000595:Amino Acid Sequence; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D007091:Image Processing, Computer-Assisted; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification*; D012015:Reference Standards
chemical_list: D010940:Plant Proteins