title: Evaluation of free radical production in an ischaemia-reperfusion model in the rabbit using a tourniquet.
abstract: Use of a tourniquet on a limb to stem blood flow is frequent in surgery. However, this results in an ischaemia-reperfusion effect that causes damage through the production of free radicals. The aim of this work was to evaluate this production of free radicals in an ischaemia-reperfusion model in the rabbit after placement of a tourniquet. Plasma lipoperoxides were measured by high-performance liquid chromatography using the malondialdehyde assay method. We found significant (P < 0.01) production of free radicals 1 min after release of the tourniquet compared with controls; no variation in levels of free radicals was observed during the ischaemia phase.
authors.affiliation: Laboratoire de Pharmacie Clinique et Biotechnique, Faculté de Pharmacie, Clermont-Ferrand, France.
authors.forename: J
authors.initials: J
authors.lastname: Chopineau
authors.forename: M F
authors.initials: MF
authors.lastname: Sommier
authors.forename: V
authors.initials: V
authors.lastname: Sautou
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005609:Free Radicals / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008954:Models, Biological; D011817:Rabbits; D015427:Reperfusion Injury / Q000097:blood*; D014111:Tourniquets / Q000009:adverse effects*
chemical_list: D005609:Free Radicals; D008315:Malondialdehyde