Encapsulation of a quinolone in liposomes. Location and effect on lipid bilayers.
The effect of a new fluoroquinolone on the distearoyl phosphatidylcholine (DSPC) bilayers was examined above and below the phase transition temperature (Tm) of the lipid. It was found by photon correlation spectroscopy that size and polydispersity of the extruded liposomes were unaffected by quinolone. Moreover, fluorescence quenching methods revealed a low fraction (13%) of non-accessible population of drug in the vesicles. This was interpreted in terms of encapsulation efficiency. However, variations in size correlated with decrease in the values of precipitation factor (P). These results reveal the instability of quinoline-DSPC liposomes beyond 5 days.
Montero
M T
MT
Unitat de Físicoquimica, Facultat de Farmacia, U.B. Spain.
Carrera
I
I
Hernandez-Borrell
J
J
D015502:Absorptiometry, Photon; D004339:Drug Compounding; D004355:Drug Stability; D008051:Lipid Bilayers; D008081:Liposomes; D009391:Nephelometry and Turbidimetry; D010316:Particle Size; D010713:Phosphatidylcholines; D015363:Quinolones / Q000008:administration & dosage / Q000737:chemistry*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013696:Temperature
D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D015363:Quinolones; C010942:1,2-distearoyllecithin