title: Cyclic AMP response in cells exposed to electric fields of different frequencies and intensities.
abstract: The action on intracellular cyclic AMP (cAMP) of therapeutically used 4000-Hz electric fields was investigated and compared with 50-Hz data. Cultured mouse fibroblasts were exposed for 5 minutes to 4000-Hz sine wave internal electric fields between 3 mV/m and 30 V/m applied within culture medium. A statistically significant decrease in cellular cAMP concentration relative to unexposed cells was observed for fields higher than 10 mV/m. The drop in cAMP was most pronounced at lower field strengths (71% of controls at 30 mV/m) and tended to disappear at higher field strengths. An increase of cAMP content was observed with 50-Hz electric fields, as was also the case when 4000-Hz fields were modulated with certain low frequencies.
authors.affiliation: Kernforschungszentrum karlsruhe, Institut für Toxikologie, Karlsruhe, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Knedlitschek
authors.forename: M
authors.initials: M
authors.lastname: Noszvai-Nagy
authors.forename: H
authors.initials: H
authors.lastname: Meyer-Waarden
authors.forename: J
authors.initials: J
authors.lastname: Schimmelpfeng
authors.forename: K F
authors.initials: KF
authors.lastname: Weibezahn
authors.forename: H
authors.initials: H
authors.lastname: Dertinger
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D000242:Cyclic AMP / Q000528:radiation effects*; D004574:Electromagnetic Fields; D051379:Mice; D015290:Second Messenger Systems / Q000528:radiation effects*
chemical_list: D000242:Cyclic AMP