title: Bacithrocins A, B and C, novel thrombin inhibitors.
abstract: Novel thrombin inhibitors, bacithrocins A, B and C, have been isolated from the culture broth of Bacillus laterosporus Laubach NR 2988. The structures of these inhibitors have been determined to be N-acyl-L-phenylalanyl-DL-arginal by the 2D-NMR experiments on their oxidation products and by amino acid analysis. Bacithrocin A inhibits thrombin, factor Xa and trypsin with IC50s of 48, 13 and 0.65 microM, respectively, which are similar to those of bacithrocins B and C. Bacithrocins prolong the clotting time induced by thrombin and factor Xa.
authors.affiliation: Nippon Roche Research Center, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kamiyama
authors.forename: T
authors.initials: T
authors.lastname: Umino
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Itezono
authors.forename: S
authors.initials: S
authors.lastname: Sawairi
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: K
authors.initials: K
authors.lastname: Yokose
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004151:Dipeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D065427:Factor Xa Inhibitors; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010206:Papain / Q000037:antagonists & inhibitors; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013917:Thrombin / Q000037:antagonists & inhibitors*; D014361:Trypsin Inhibitors
chemical_list: D004151:Dipeptides; D065427:Factor Xa Inhibitors; D014361:Trypsin Inhibitors; C089887:bacithrocin A; C089888:bacithrocin B; C089889:bacithrocin C; D013917:Thrombin; D010206:Papain