[Are desquamated trophoblastic cells retrieved from the cervix suitable for a prenatal diagnosis?].
Prenatal diagnosis based on sampling of fetal tissues, amniotic fluid or chorionic villi is associated with the risk of miscarriage and fetal damage. These risks would be avoided if diagnosis could be performed in desquamated trophoblast cells recovered non-invasively from the maternal cervix. We report on our experience of fetal karyotyping on endocervical lavage using in situ hybridization (FISH) and DNA amplification (PCR) fetal sex was correctly predicted in 8/10 cases by FISH and in 6/10 by PCR. FISH appeared to be a reliable technique for karyotyping when trophoblast can be recovered from the maternal endocervix (8/10).
Ville
Y
Y
Service de gynécologie-obstétrique et Laboratoire de fécondation in vitro, Hôpital A. Béclère, Clamart.
Lochu
P
P
Rhali
H
H
D'Auriol
L
L
Bedossa
P
P
Bergère
M
M
Baud
M
M
Selva
J
J
Chaouat
G
G
Nicolaides
K
K
D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping / Q000379:methods*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D015203:Reproducibility of Results; D012732:Sex Determination Analysis / Q000379:methods*; D007507:Therapeutic Irrigation / Q000379:methods*; D014327:Trophoblasts / Q000166:cytology*; D014626:Vaginal Smears
La desquamation de cellules trophoblastiques recueillies au niveau du col utérin permet-elle un diagnostic prénatal?