Diuretic effect of NG-nitro-L-arginine methyl ester in the rat.
Intravenous infusion of the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester, L-NAME (10 micrograms kg-1 min-1), to anaesthetized rats produced a diuresis and natriuresis. By contrast, infusion of the same dose of NG-nitro-D-arginine methyl ester had no effect on either urine output or sodium excretion. The effects of L-NAME were first evident 120 min after the start of infusion and by 170 min a fivefold increase in urine volume and sodium excretion was recorded. L-NAME also produced a transient fall in inulin clearance and a persistent decline in renal blood flow. These renal effects of L-NAME were associated with a gradual elevation of mean arterial blood pressure, although this only attained statistical significance, in comparison with saline-infused animals, 170 min after the start of infusion. The findings indicate the diuresis and natriuresis evoked by L-NAME in the rat is a result of a direct tubular action together with a pressure diuresis.
Li
Q
Q
Department of Pharmacology, University of Leeds, UK.
Bowmer
C J
CJ
Yates
M S
MS
D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D004232:Diuretics / Q000008:administration & dosage / Q000494:pharmacology*; D007262:Infusions, Intravenous; D007444:Inulin / Q000378:metabolism; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000187:drug effects
D004232:Diuretics; D009569:Nitric Oxide; D007444:Inulin; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester