[Fixation and decalcification in trephination biopsy].
We tested several modifications of paraffin embedding technique when examining bone marrow biopsies in order to achieve an optimal combination of morphology and antigen preservation. Short pre-fixation in formalin followed by mixture of formalin and 10% formic acid were found optimal for quick tissue processing where electron microscopy was not planned. Formalin pre-fixation prevents the occurrence of imperfect stainability of superficial parts of trephine cylinders caused by an effect of formic acid on non-fixed bone marrow tissues. Decalcification in mixture of formol and EDTA is recommended if there is time enough. The supersensitive streptavidin-biotin detection system with alkaline phosphatase was found to provide the best results.
Fakan
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Siklův patologicko-anatomický ústav LF UK, Plzen.
Skálová
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A
D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology*; D003648:Decalcification Technique; D006801:Humans; D016612:Paraffin Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods*
Fixace a dekalcifikace trepanobiopsií.