Quantitative analysis of superoxide anion generation in living cells by using chemiluminescence video microscopy.
Superoxide anions (O2-) generated by rabbit neutrophils were detected and quantified by a video microscope equipped with a photon-counting camera. One count obtained by this system was equivalent to 59 amol of O2-. Maximum O2- production was observed at 6-8 min after stimulation and was estimated as 1.9 fmol/min per cell on the average.
Suzaki
E
E
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
Kawai
E
E
Kodama
Y
Y
Suzaki
T
T
Masujima
T
T
D000818:Animals; D008163:Luminescent Measurements; D018715:Microscopy, Video / Q000379:methods*; D009504:Neutrophils / Q000737:chemistry*; D011817:Rabbits; D013481:Superoxides / Q000032:analysis*
D013481:Superoxides