Cloning and sequencing of a K+ transport gene (trk A) from the marine bacterium Vibrio alginolyticus.
A gene has been cloned from the marine bacterium Vibrio alginolyticus that functionally complements a mutant strain of Escherichia coli, TK420, defective in K+ transport genes (kdpABC, trkD, trkA). The cloned Vibrio gene allowed TK420 to grow in a synthetic medium containing less than 10 mM K+ and concomitantly led to an increase in K+ uptake activity. The nucleotide sequence of the cloned fragment revealed an open reading frame, which encodes a protein with a predicted 458 amino acid sequence and molecular mass of 50,122 Da. This gene has 71% homology to trkA gene at the DNA level from E. coli and the deduced amino acid sequence is 79% identical with E. coli TrkA, implying that V. alginolyticus has a trkA-like gene as a component of K+ transport systems.
Nakamura
T
T
Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Matsuba
Y
Y
Yamamuro
N
N
Booth
I R
IR
Unemoto
T
T
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D008386:Marine Biology; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011188:Potassium / Q000378:metabolism*; D020917:Receptor, trkA; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000235:genetics*; D014871:Water Microbiology
D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D020917:Receptor, trkA; D011188:Potassium