Posttransplantation function of hearts preserved with fluorochemical emulsion.
This study was designed to determine whether the novel perfluoroperhydrophenanthrene-egg yolk phospholipid emulsion, APE-LM, was an effective oxygen carrier for long-term hypothermic heart preservation. We postulated that hearts preserved with APE-LM would be well oxygenated during 24-hour preservation and that reperfusion of such hearts with blood would not produce functional or metabolic evidence of myocardial ischemia. Four groups of rabbit hearts were studied (n = 7 per group): fresh controls: nonpreserved, nontransplanted hearts; surgical controls: fresh hearts transplanted heterotopically for 75 minutes before explant and study for 4 hours as isolated working hearts perfused at 37 degrees C; crystalloid-preserved: hearts preserved with crystalloid medium, followed by transplantation and isolated heart perfusion; APE-LM-preserved: hearts treated as those in the crystalloid-preserved group, but preservation was with medium containing APE-LM emulsion (10 ml/dl). Preservation was with continuous coronary perfusion at 18 mm Hg pressure, 12 degrees C, and oxygen tension 838 +/- 11 mm Hg. During preservation, APE-LM hearts had significantly higher pyruvate consumption, and correspondingly higher oxygen consumption, than that of crystalloid hearts. No significant differences were found among fresh controls, surgical controls, and APE-LM-preserved hearts with respect to contractile or output function, oxygen consumption and efficiency indexes, or lactate production during in vitro perfusion. Left ventricular peak systolic pressure and peak rate of pressure development were significantly lower for crystalloid-preserved hearts than for fresh and surgical controls. Left ventricular end-diastolic pressure of crystalloid-preserved hearts was higher than that of the other three groups. The data indicate that rabbit hearts in this model were well preserved with APE-LM and that this emulsion produced better recovery of function than did crystalloid preservation, possibly as a consequence of the high oxygen delivery by the fluorocarbon during preservation.
Segel
L D
LD
Department of Surgery, University of California School of Medicine, Davis 95616.
Follette
D M
DM
Iguidbashian
J P
JP
Contino
J P
JP
Castellanos
L M
LM
Berkoff
H A
HA
Kaufman
R J
RJ
Schweighardt
F K
FK
D000818:Animals; D002314:Cardioplegic Solutions; D005466:Fluorocarbons; D016027:Heart Transplantation / Q000502:physiology*; D007036:Hypothermia, Induced; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000378:metabolism; D009926:Organ Preservation; D010743:Phospholipids; D011817:Rabbits; D013997:Time Factors; D016276:Ventricular Function / Q000502:physiology
C076991:APE-LM; D002314:Cardioplegic Solutions; D005466:Fluorocarbons; D010743:Phospholipids