Differential integrin expression facilitates isolation of human fetal pancreatic epithelial cells.
We have studied the expression of the beta 1 family of integrins in fetal and adult human pancreas. Immunohistochemical staining with a monoclonal anti-beta 1 antibody revealed that the epithelial cells of the human fetal pancreas express high amounts of beta 1 integrin, while the pancreatic stromal cells express substantially lower amounts. Islets of Langerhans from human adult pancreas also expressed high amounts of beta 1 integrin. Taking advantage of the extremely high affinity binding between the invasin protein of Yersinia pseudotuberculosis and many beta 1 integrins, we have been able to isolate highly enriched populations of fetal pancreatic epithelial cells. Epithelial-enriched cell populations retain the ability to differentiate into mature endocrine cells following transplantation into nude mice.
Levine
F
F
Department of Pediatrics, UCSD School of Medicine, La Jolla 92093-0634.
Beattie
G M
GM
Hayek
A
A
D018829:Adhesins, Bacterial; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D017690:Cell Transplantation; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005333:Fetus / Q000033:anatomy & histology; D005347:Fibroblasts; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D015011:Yersinia pseudotuberculosis / Q000737:chemistry
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D016023:Integrins; C053476:invasin, Yersinia