Engraftment of HTLV-I-transformed human T-cell line into SCID mice with NK cell function.
HTLV-I-transformed human T-cell line, MT-2, has been reported to successfully proliferate in only the SCID mice with depletion of NK cell function. However, MT-2 cells could be engrafted into SCID mice possessing normal NK cell function in this study. MT-2 cells (5-7 x 10(7) cells) were intraperitoneally injected into SCID mice without the deletion of NK cell function. The SCID mice developed tumors in the peritoneal cavities 3 months after the inoculation of MT-2 cells. All tumors reacted to anti-HTLV-I p19 and anti-HLA-DR monoclonal antibodies by immunofluorescence assay and were also positive for the HTLV-I gene by PCR assay. DNA obtained from main organs in group of mice with or without tumors showed a high incidence of positive signals for HTLV-I gene by PCR assay.
Ohsugi
T
T
Department of Virology, Kurume University School of Medicine, Osaka University Medical School, Japan.
Ishibashi
K
K
Shingu
M
M
Nomura
T
T
D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007668:Kidney / Q000821:virology; D007694:Killer Cells, Natural / Q000276:immunology*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology* / Q000821:virology; D008099:Liver / Q000821:virology; D008168:Lung / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D017710:Lymphocyte Transfusion; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D013154:Spleen / Q000821:virology; D013601:T-Lymphocytes; D014183:Transplantation, Heterologous
D017931:DNA Primers; D004279:DNA, Viral