Detection of 500-nucleotide DNA by laser desorption mass spectrometry.
We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.
Tang
K
K
Oak Ridge National Laboratory, Chemical Physics Section, Tennessee 37831-6378.
Taranenko
N I
NI
Allman
S L
SL
Cháng
L Y
LY
Chen
C H
CH
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010848:Picolinic Acids
D007135:Immunoglobulin Variable Region; D010848:Picolinic Acids; C049246:3-hydroxypicolinic acid; D004247:DNA