The physicochemical, immunological and biological properties of rat pituitary and plasma LH.
A comparison was made between the properties of LH derived from female rat pituitary glands and plasma. Samples were collected from adult intact rats 5 h before or at the time of the pro-oestrous preovulatory LH surge; 27-day-old rats untreated or given 5 IU pregnant mares' serum gonadotrophin (PMSG) s.c. on day 25, which induced LH release 54 h later and adult ovariectomized rats untreated or primed with either 5 micrograms oestradiol benzoate s.c. or 5 micrograms oestradiol benzoate followed 48 h later by 0.5 mg progesterone s.c., which induced LH release 4-6 h later. All pituitary LH samples were totally bound to an anionic ion-exchange resin (DE52), while only a small proportion of the plasma LH was bound. Only 0-10% plasma LH obtained from intact, ovariectomized (with and without steroids) and untreated immature rats was bound, while a greater proportion of bound LH (36%) was noted in rats treated with PMSG. Gel filtration indicated only slight differences between pituitary and plasma LH, the former eluting marginally earlier than whole plasma and the unbound and bound plasma forms derived after separation by DE52 resin. Affinity chromatography (Concanavalin A and Glycine maximus) showed that LH from both sources possesses high mannose oligosaccharides and that plasma LH does not bear terminal N-acetyl galactosamine residues, although 20% of the pituitary form does. Plasma obtained from pro-oestrous rats had greater bioactivity than had pituitary LH in stimulating testosterone from Leydig cells and progesterone from granulosa cells in vitro, and inducing ovulation in immature rats in vivo. Leydig cell bioassays for LH in fractions obtained from ion-exchange separation indicate that steroidogenic activity of unbound plasma LH is greater than bound pituitary LH when they were collected at times of enhanced release. When release was inhibited (oestrogen-primed ovariectomized rats or immature rats), the steroidogenic activity of plasma and pituitary LH were similar and an acidic steroidogenic component was present in the plasma that was not recognized immunogenically as LH. In summary, pituitary LH undergoes a conversion on release into the plasma that involves a change in binding characteristics on an ion-exchange resin. In conditions when LH release is enhanced there is an increase in bioactivity of plasma LH owing to modification either by steroids or some other plasma factor(s) that perhaps influence the structure of LH directly or by steroids acting indirectly to alter GnRH release, which then modifies LH structure. These structural changes are minor and probably involve alterations in the glycosyl attachments.
Leigh
A J
AJ
Department of Obstetrics and Gynaecology, St George's Hospital Medical School, London, UK.
Chapman
A J
AJ
Shakil
T
T
Wilson
C A
CA
D000818:Animals; D001681:Biological Assay; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D007986:Luteinizing Hormone / Q000097:blood / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D010060:Ovulation; D010902:Pituitary Gland / Q000737:chemistry / Q000378:metabolism*; D011359:Proestrus / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar
D007986:Luteinizing Hormone