Mycobacterial lipoarabinomannan induces nitric oxide and tumor necrosis factor alpha production in a macrophage cell line: down regulation by taurine chloramine.
Avirulent mycobacterium H37Ra lipoarabinomannan (LAM) elicited nitric oxide (NO) and tumor necrosis factor alpha in a dose-dependent manner in a murine macrophage cell line, RAW 264.7 cells. H37Ra LAM and recombinant gamma interferon were highly synergistic for NO production. The production of NO and the release of tumor necrosis factor alpha stimulated by H37Ra LAM plus recombinant gamma interferon in RAW 264.7 cells are inhibited by taurine chloramine.
Schuller-Levis
G B
GB
Staten Island University Hospital, New York 10305.
Levis
W R
WR
Ammazzalorso
M
M
Nosrati
A
A
Park
E
E
D000818:Animals; D002460:Cell Line; D015536:Down-Regulation; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009161:Mycobacterium / Q000472:pathogenicity*; D009569:Nitric Oxide / Q000096:biosynthesis*; D011994:Recombinant Proteins; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C050016:lipoarabinomannan; D013654:Taurine; D009569:Nitric Oxide; C043410:N-chlorotaurine; D007371:Interferon-gamma