Pollutant degradation by white rot fungi.
The white rot fungi technology is very different from other more well-established methods of bioremediation (e.g., bacterial systems). The differences are primarily due to the mechanisms discussed previously. The unusual mechanisms used by the fungi provide them with several advantages for pollutant degradation, but the complexity of these mechanisms has also made the technology slow to emerge as a viable method of bioremediation. One distinct advantage that white rot fungi have over bacterial systems is that they do not require preconditioning to a particular pollutant. Bacteria must be preexposed to a pollutant to allow the enzymes that degrade the pollutant to be induced. The pollutant must also be present in a significant concentration, otherwise induction of enzyme synthesis will not occur. Therefore, there is a finite level to which pollutants can be degraded by bacteria. In contrast, the degradative enzymes of white rot fungi are induced by nutrient limitation. Thus, cultivate the fungus on a nutrient that is limited in something, and the degradative process will be initiated. Also, because the induction of the lignin-degrading system is not dependent on the chemical, pollutants are degraded to near-nondetectable levels by white rot fungi. Another unique feature of pollutant degradation by white rot fungi involves kinetics. The process of chemical conversion by these fungi occurs via a free-radical process, and thus the degradation of chemicals often follows pseudo-first-order kinetics. In fact, in several studies, it has been found that the rate of mineralization or disappearance of a pollutant is proportional to the concentration of the pollutant. This makes the time required to achieve decontamination more important than the rate of degradation. Because the metabolism of chemicals by bacteria involves mostly enzymatic conversions, pollutant degradation often follows Michaelis-Menton-type kinetics. Therefore, Km values of various degradative enzymes with respect to the pollutant must be considered when using bacteria for bioremediation. Considering this, the solubility of a pollutant or a mixture of pollutants might also present a problem for bacterial degradation. In contrast, using a nonspecific free-radical-based mechanism, the fungi are able to degrade insoluble complex mixtures of pollutants, such as creosote (Aust and Bumpus 1989) and Arochlor (Bumpus and Aust 1987b). Inexpensive nutrient sources, such as sawdust, wood chips, surplus grains, and agricultural wastes, can be used to effectively cultivate white rot fungi.(ABSTRACT TRUNCATED AT 400 WORDS)
Barr
D P
DP
Biotechnology Center, Utah State University, Logan 84322-4705.
Aust
S D
SD
D001487:Basidiomycota / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000378:metabolism*; D006256:Hazardous Waste; D008031:Lignin / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism
D004785:Environmental Pollutants; D006256:Hazardous Waste; D008031:Lignin; D010544:Peroxidases