Cyclic AMP response in cells exposed to electric fields of different frequencies and intensities.
The action on intracellular cyclic AMP (cAMP) of therapeutically used 4000-Hz electric fields was investigated and compared with 50-Hz data. Cultured mouse fibroblasts were exposed for 5 minutes to 4000-Hz sine wave internal electric fields between 3 mV/m and 30 V/m applied within culture medium. A statistically significant decrease in cellular cAMP concentration relative to unexposed cells was observed for fields higher than 10 mV/m. The drop in cAMP was most pronounced at lower field strengths (71% of controls at 30 mV/m) and tended to disappear at higher field strengths. An increase of cAMP content was observed with 50-Hz electric fields, as was also the case when 4000-Hz fields were modulated with certain low frequencies.
Knedlitschek
G
G
Kernforschungszentrum karlsruhe, Institut für Toxikologie, Karlsruhe, Germany.
Noszvai-Nagy
M
M
Meyer-Waarden
H
H
Schimmelpfeng
J
J
Weibezahn
K F
KF
Dertinger
H
H
D000818:Animals; D002461:Cell Line, Transformed; D000242:Cyclic AMP / Q000528:radiation effects*; D004574:Electromagnetic Fields; D051379:Mice; D015290:Second Messenger Systems / Q000528:radiation effects*
D000242:Cyclic AMP