Effects of lipopolysaccharide on human placental prostaglandin F2 alpha production in vitro.
The effect of bacterial endotoxin (lipopolysaccharide, LPS), on the release of prostaglandin F2 alpha (PGF2 alpha) from human placental explants was investigated. Both LPS and calcium ionophore A23187 stimulated PGF2 alpha release (p < 0.05). Placental explants, either exposed to LPS and A23187 simultaneously or primed by LPS before A23187 stimulation, resulted in similar increased PGF2 alpha releases, which were higher than the level of LPS alone. The data suggest LPS could increase basal PGF2 alpha release from human placenta, combined with A23187 it may act on PG synthase as well in favour of PGF2 alpha formation.
Gu
W
W
Monash University Department of Obstetrics and Gynaecology, Clayton, Vic., Australia.
Rice
G E
GE
Brennecke
S P
SP
D000001:Calcimycin / Q000494:pharmacology; D015237:Dinoprost / Q000096:biosynthesis / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009924:Organ Culture Techniques; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D013268:Stimulation, Chemical; D013997:Time Factors
D008070:Lipopolysaccharides; D000001:Calcimycin; D015237:Dinoprost; D011451:Prostaglandin-Endoperoxide Synthases