Tissue-specific expression of an oat 12S seed globulin gene in developing tobacco seeds: differential mRNA and protein accumulation.
We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and in situ hybridization. However, immunological tissue printing detected the oat protein exclusively in the tobacco endosperm, suggesting that extensive post-transcriptional regulatory processes influence the expression of the monocot transgene in the dicot host.
Schubert
R
R
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Panitz
R
R
Manteuffel
R
R
Nagy
I
I
Wobus
U
U
Bäumlein
H
H
D000485:Allergens; D052179:Antigens, Plant; D018554:Avena / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D018519:Meristem / Q000737:chemistry; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012231:Rhizobium / Q000235:genetics; D055314:Seed Storage Proteins; D012639:Seeds / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000737:chemistry / Q000235:genetics*
D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D055314:Seed Storage Proteins; C059777:cruciferin protein, Brassica napus; D005966:Glucuronidase