title: Administration of prostaglandin E1 after lung transplantation improves early graft function.
abstract: Early graft dysfunction continues to be a major clinical problem after lung transplantation. The objective of this experiment was to determine whether continuous administration of prostaglandin E1 (PGE1) after lung transplantation has a beneficial effect on early graft function. Left lung allotransplantation was performed in 10 size-matched mongrel dogs (weight, 24.4 to 31.4 kg). Lung preservation consisted of a bolus injection of PGE1 (250 micrograms) into the pulmonary artery, followed by a pulmonary artery flush with 50 mL/kg of 4 degrees C modified Euro-Collins solution. The lungs were then stored at 1 degree C for 12 hours. Left lung transplantation was performed using standard technique. The right pulmonary artery and right bronchus were ligated prior to chest closure. Animals were placed in the supine position and ventilated for 6 hours with 100% oxygen at a rate of 20 breaths/min, a tidal volume of 550 mL, and a positive end-expiratory pressure of 5 cm H2O. Animals were randomly allocated to one of two groups. Group I animals (n = 6) received continuous PGE1 infusion from the onset of implantation. The dose was gradually increased and fixed when mean systemic pressure showed a 10% decrease (mean PGE1 dose, 31.7 +/- 6.9 ng.kg-1.min-1). Group II animals (n = 4) received no PGE1. After the 6-hour assessment period, arterial oxygen tension and alveolar-arterial oxygen pressure difference were preserved in group I compared with group II (group I versus group II: arterial oxygen tension, 255.8 +/- 37.6 mm Hg versus 64.7 +/- 7.9 mm Hg [p < 0.05]; alveolar-arterial oxygen pressure difference, 411.1 +/- 70.5 mm Hg versus 597.5 +/- 1.3 mm Hg [p < 0.05]).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, Washington University School of Medicine, Barnes Hospital, St. Louis, Missouri 63110.
authors.forename: M
authors.initials: M
authors.lastname: Aoe
authors.forename: G D
authors.initials: GD
authors.lastname: Trachiotis
authors.forename: K
authors.initials: K
authors.lastname: Okabayashi
authors.forename: J K
authors.initials: JK
authors.lastname: Manchester
authors.forename: O H
authors.initials: OH
authors.lastname: Lowry
authors.forename: J D
authors.initials: JD
authors.lastname: Cooper
authors.forename: G A
authors.initials: GA
authors.lastname: Patterson
mesh_terms: D000527:Alprostadil / Q000008:administration & dosage*; D000818:Animals; D015925:Cryopreservation / Q000379:methods; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D006439:Hemodynamics / Q000187:drug effects; D006982:Hypertonic Solutions; D007269:Injections, Intra-Arterial; D008168:Lung / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D016040:Lung Transplantation / Q000502:physiology*; D008176:Lung Volume Measurements; D009195:Peroxidase / Q000378:metabolism; D011182:Postoperative Care; D011183:Postoperative Complications / Q000453:epidemiology / Q000503:physiopathology; D011652:Pulmonary Circulation / Q000187:drug effects; D011654:Pulmonary Edema / Q000453:epidemiology / Q000503:physiopathology; D011659:Pulmonary Gas Exchange / Q000187:drug effects; D011897:Random Allocation; D012121:Respiration, Artificial; D013997:Time Factors
chemical_list: C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D009195:Peroxidase; D000527:Alprostadil