title: Experimental assessment of spectrum analysis of ultrasonic echoes as a method for estimating scatterer properties.
abstract: In vitro experiments using weak scatterers ranging in size from mean longest diameter of 26.9 to 83.0 microns were performed to test the validity of theoretical predictions for scatterer size and concentration derived from normalized power spectrum parameters of ultrasonic backscatterer echoes. Scatterers consisting of cell clusters were suspended in collagen gel and scanned by a 10 MHz transducer system. Optical measurements validated theoretical predictions that (1) slope value is a function solely of scatterer size; (2) intercept value is a function of scatterer size and concentration; and (3) midband fit value increases as scatterer concentration increases, and, to a lesser extent, as size increases. These results were obtained under relatively ideal conditions of minimal attenuation and scatterer spacing (not closer than two scatterer diameters) and were consistent with the assumptions underlying the scattering theory.
authors.affiliation: Department of Surgery, Medical College of Pennsylvania, Philadelphia 19129.
authors.forename: T
authors.initials: T
authors.lastname: Hosokawa
authors.forename: B
authors.initials: B
authors.lastname: Sigel
authors.forename: J
authors.initials: J
authors.lastname: Machi
authors.forename: H
authors.initials: H
authors.lastname: Kitamura
authors.forename: R V
authors.initials: RV
authors.lastname: Kolecki
authors.forename: J R
authors.initials: JR
authors.lastname: Justin
authors.forename: E J
authors.initials: EJ
authors.lastname: Feleppa
authors.forename: G
authors.initials: G
authors.lastname: Tuszynski
authors.forename: T
authors.initials: T
authors.lastname: Kakegawa
mesh_terms: D006801:Humans; D008175:Lung Neoplasms / Q000000981:diagnostic imaging; D014407:Tumor Cells, Cultured; D014465:Ultrasonics; D014463:Ultrasonography / Q000379:methods*