Novel radioligands for the dopamine transporter demonstrate the presence of intrastriatal nigral grafts in the MPTP-treated monkey: correlation with improved behavioral function.
This preliminary study demonstrates by in vitro autoradiography the value of novel radiolabeled cocaine analogs to characterize the neurochemical identity and measure the density and growth of fetal ventral mesencephalic cells transplanted to the caudate nucleus of the adult MPTP-treated monkey. Two cocaine derivatives were used, one with extremely high affinity for both dopamine and serotonin transporters and one which was selective for the dopamine transporter. The results suggest that the transplantation procedure was able to increase the density of transporter sites in most of the caudate nucleus, although the proportion of dopaminergic and serotonergic fibers may have been altered from normal.
Elsworth
J D
JD
Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06510.
al-Tikriti
M S
MS
Sladek
J R
JR
Taylor
J R
JR
Innis
R B
RB
Redmond
D E
DE
Roth
R H
RH
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000818:Animals; D001345:Autoradiography; D016380:Brain Tissue Transplantation / Q000502:physiology; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D002522:Chlorocebus aethiops; D003042:Cocaine / Q000031:analogs & derivatives* / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D050483:Dopamine Plasma Membrane Transport Proteins; D016332:Fetal Tissue Transplantation / Q000502:physiology; D007457:Iodine Radioisotopes; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D010302:Parkinson Disease, Secondary / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D011869:Radioligand Assay; D050486:Serotonin Plasma Membrane Transport Proteins; D013378:Substantia Nigra / Q000378:metabolism / Q000502:physiology / Q000637:transplantation*; D016042:Transplantation, Heterotopic; D014184:Transplantation, Homologous
D002352:Carrier Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D050486:Serotonin Plasma Membrane Transport Proteins; C079213:RTI 121; C069723:2beta-carbomethoxy-3beta-(4-iodophenyl)tropane; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D003042:Cocaine