title: Carrier-state infection of feline T-lymphoblastoid cells with feline calicivirus.
abstract: The susceptibility of feline T lymphocytes to feline calicivirus (FCV) in vitro was investigated using feline T-lymphoblastoid cell lines, namely MYA-1 and FL74 cells. The virus titers of supernatants in FCV-infected MYA-1 and FL74 cell cultures increased rapidly, and FCV antigens were also detected in the FCV-infected cells. There were slight differences in the molecular weights of capsid proteins expressed in FCV-infected MYA-1, FL74 and Crandell feline kidney cells. MYA-1 and FL74 cells were productively and persistently infected with FCV, and FCV antigens were observed in the FCV-infected cells for more than one month. At 3 months post infection, FCV-infected FL74 cells that stopped producing infectious FCV could be reinfected with FCV. However, no cytopathic effects were observed.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Tohya
authors.forename: T
authors.initials: T
authors.lastname: Horimoto
authors.forename: K
authors.initials: K
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D017250:Caliciviridae Infections / Q000662:veterinary* / Q000821:virology; D017927:Calicivirus, Feline / Q000378:metabolism / Q000502:physiology*; D002213:Capsid / Q000096:biosynthesis; D002353:Carrier State / Q000662:veterinary* / Q000821:virology; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D002460:Cell Line; D013601:T-Lymphocytes / Q000821:virology; D013997:Time Factors; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral