Laser-induced fluorescence in malignant and normal tissue in mice injected with two different carotenoporphyrins.
Laser-induced fluorescence (LIF) was used to characterise the localisation of an intravenously administered trimethylated carotenoporphyrin [CP(Me)3] and a trimethoxylated carotenoporphyrin [CP(OMe)3] in an intramuscularly transplanted malignant tumour (MS-2 fibrosarcoma) and healthy muscle in female Balb/c mice, 3, 24, 48 and 96 h post injection. The fluorescence was induced with a dye laser pumped by a nitrogen laser, emitting light at 425 nm. The fluorescence spectra were recorded in the region 455-760 nm using a polychromator equipped with an image-intensified CCD camera. The tumour/peritumoral muscle ratio was about 5:1 for CP(Me)3 and about 6:1 for CP(OMe)3 in terms of the background-free fluorescence intensity, which peaked at about 655 nm. By including the endogenous tissue fluorescence, the contrast was further enhanced by a factor of approximately 2.
Nilsson
H
H
Lund University Medical Laser Center, Sweden.
Johansson
J
J
Svanberg
K
K
Svanberg
S
S
Jori
G
G
Reddi
E
E
Segalla
A
A
Gust
D
D
Moore
A L
AL
Moore
T A
TA
D000818:Animals; D002338:Carotenoids / Q000032:analysis* / Q000493:pharmacokinetics; D005260:Female; D005354:Fibrosarcoma / Q000175:diagnosis* / Q000378:metabolism*; D005453:Fluorescence; D007834:Lasers; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000378:metabolism*; D009368:Neoplasm Transplantation; D011166:Porphyrins / Q000032:analysis* / Q000493:pharmacokinetics; D011838:Radiation-Sensitizing Agents / Q000032:analysis* / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence
D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C090538:trimethoxycarotenoporphyrin; C090537:trimethylcarotenoporphyrin; D002338:Carotenoids