title: Antigen recognition and activation of ovine gamma delta T cells.
abstract: We have investigated several aspects of gamma delta T cells in sheep. gamma delta T cells of sheep express a unique transmembrane protein termed T19 but lack the expression of particular cell-surface molecules such as CD2, CD4 and CD8 which are typically associated with alpha beta T cells. The majority of gamma delta T cells isolated from animals of all ages examined lacked the expression of CD45RA. A faster rate of activation by gamma delta T cells compared to either CD4 or CD8 T cells was seen in the time-course of IL-2 receptor alpha chain (CD25) cell-surface expression. All gamma delta T cells expressed the CD25 protein within 8 hr of activation whereas the majority of CD4 or CD8 T cells did not express CD25 until 24 hr post-concanavalin A (Con A) stimulation. This difference in the rate of expression of activation molecules was not restricted to CD25, as a similar trend was seen with cell-surface expression of major histocompatibility complex (MHC) class II molecules. We have used the distinct phenotypic profile of ovine gamma delta T cells to purify these cells by positive selection via the T19 molecule to assess their in vitro proliferative response to various antigens. Routinely, cell populations comprising more than 93% gamma delta T cells with yields of approximately 55% were obtained. Purified gamma delta T cells were capable of responding to Mycobacterium tuberculosis antigen in a primary and secondary in vitro proliferation assay and to ovalbumin in a secondary response. Ovine gamma delta T cells showed little, if any, proliferative response to allogeneic stimulator cells.
authors.affiliation: Department of Veterinary Pathology, University of Edinburgh.
authors.forename: C W
authors.initials: CW
authors.lastname: Evans
authors.forename: B T
authors.initials: BT
authors.lastname: Lund
authors.forename: I
authors.initials: I
authors.lastname: McConnell
authors.forename: R
authors.initials: R
authors.lastname: Bujdoso
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D015703:Antigens, CD / Q000097:blood; D002455:Cell Division / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D003208:Concanavalin A / Q000276:immunology; D005260:Female; D017493:Leukocyte Common Antigens / Q000097:blood; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D012756:Sheep / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000941:Antigens; D015703:Antigens, CD; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2; D003208:Concanavalin A; D017493:Leukocyte Common Antigens