Photoprotection of normal human hematopoietic progenitors by the tetrapeptide N-AcSDKP.
The tetrapeptide AcSDKP (Ser-Asp-Lys-Pro) is a reversible inhibitor of normal human hematopoietic progenitor growth. In this paper, we report that preincubation of bone-marrow mononuclear cells (MNC) with AcSDKP at 10(-10) M for 20 hours protects the granulocyte-macrophage colony-forming unit (CFU-GM) progenitors against photofrin II-mediated phototherapy. This protective effect was observed after short-term exposure to photofrin (2.5 micrograms/mL) and irradiation by high-energy doses at 514 nm. Nevertheless, AcSD-KP, which has no effect on leukemic cell proliferation, does not protect the HL-60 and K-562 leukemic cell lines against photosensitization.
Coutton
C
C
Institut d'Hématologie et d'Immunologie, Hospices civils, Université Louis Pasteur, Strasbourg, France.
Guigon
M
M
Bohbot
A
A
Ferrani
K
K
Oberling
F
F
D000595:Amino Acid Sequence; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D017323:Dihematoporphyrin Ether / Q000037:antagonists & inhibitors*; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000166:cytology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology*; D011837:Radiation-Protective Agents; D014407:Tumor Cells, Cultured
D009842:Oligopeptides; D011837:Radiation-Protective Agents; D017323:Dihematoporphyrin Ether; C058504:goralatide