DNA-protein complexes. Natural targets for DNA-hydrolyzing antibodies.
Sera of patients with different types of leukemia and acquired immune deficiency syndrome (AIDS) have been examined for the presence of the anti-DNA antibodies. DNA-hydrolyzing activity of antibodies was detected in the sera of patients with chronic lymphoid leukemia (CLL), pre-B-cell acute lymphoid leukemia (pre-B-All), acute myeloleukosis (AML), and AIDS in stages III and IV of the disease. In immunofluorescence tests, the DNA-hydrolyzing antibodies reacted preferentially with proliferating cell nuclei compared with resting cells. A 35-kDa factor was identified as the target for the DNA antibodies in the cell nuclei. The DNA-hydrolyzing antibody fraction from the serum of an AIDS patient crossreacted with HIV I virus proteins gp160, gp120, and p65.
Gololobov
G V
GV
Englehardt Institute of Molecular Biology, Russian Academy of Sciences.
Mikhalap
S V
SV
Starov
A V
AV
Kolesnikov
A F
AF
Gabibov
A G
AG
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D017151:Antibodies, Catalytic / Q000276:immunology / Q000378:metabolism*; D001323:Autoantibodies / Q000276:immunology / Q000378:metabolism*; D002455:Cell Division; D003429:Cross Reactions; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015684:Gene Products, pol / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D006801:Humans; D006868:Hydrolysis; D007938:Leukemia / Q000276:immunology*; D009363:Neoplasm Proteins; D012795:Sialoglycoproteins / Q000276:immunology; D014407:Tumor Cells, Cultured; D054302:pol Gene Products, Human Immunodeficiency Virus
D017151:Antibodies, Catalytic; D001323:Autoantibodies; D004268:DNA-Binding Proteins; D015684:Gene Products, pol; D015699:HIV Envelope Protein gp120; C060467:HIV pol protein p65; D009363:Neoplasm Proteins; D012795:Sialoglycoproteins; D054302:pol Gene Products, Human Immunodeficiency Virus; D004247:DNA