Comparison of peptide analysis results for horse, bovine and dog cytochrome c using capillary electrophoresis/mass spectrometry with untreated capillary column and basic buffer condition.
Peptide analysis of cytochrome c using capillary electrophoresis/mass spectrometry was demonstrated to be a rapid, sensitive and useful technique for providing information concerning the location of variation. We analyzed tryptic digests of horse, bovine and dog cytochrome c, and compared the peptide analysis results for information concerning amino acid sequences in proteins. The differences in amino acid sequence were confirmed by taking into consideration that all the other fragments are identical in each map. The use of an untreated capillary column with basic buffer condition (water+acetonitrile+(20 g/L) ammonium carbonate (70/20/10), pH 9.2) had a reproducibility of migration times to within about 2%.
Takada
Y
Y
Central Research Laboratory, Hitachi Ltd, Tokyo, Japan.
Nakayama
K
K
Yoshida
M
M
Sakairi
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002021:Buffers; D002417:Cattle; D003574:Cytochrome c Group / Q000737:chemistry*; D004285:Dogs; D004586:Electrophoresis; D006736:Horses; D006868:Hydrolysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D014108:Tosylphenylalanyl Chloromethyl Ketone; D014357:Trypsin
D002021:Buffers; D003574:Cytochrome c Group; D014108:Tosylphenylalanyl Chloromethyl Ketone; D014357:Trypsin