title: Chromosomal and temperature-dependent sex determination: the search for a conserved mechanism.
abstract: To test the hypothesis that the same (or similar) genes may be involved in chromosomal sex determination and temperature dependent sex determination we investigated the expression of the mammalian testis determining gene SRY in the American alligator (Alligator mississippiensis), a species with temperature dependent sex. We have cloned Alligator SRY-related genes (A-Srys) which constitute three major gene classes with high degrees of sequence homology (45-87%) to mammalian SRY and related genes. Expression analysis and cellular localisation studies using staged embryonic material from male and female incubation temperatures are underway to assess the role of A-Sry genes in temperature dependent sex determination.
authors.affiliation: Department of Craniofacial Development, UMDS, Guys Hospital, London, United Kingdom.
authors.forename: A M
authors.initials: AM
authors.lastname: Coriat
authors.forename: E
authors.initials: E
authors.lastname: Valleley
authors.forename: M W
authors.initials: MW
authors.lastname: Ferguson
authors.forename: P T
authors.initials: PT
authors.lastname: Sharpe
mesh_terms: D000489:Alligators and Crocodiles / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D005260:Female; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation / Q000235:genetics / Q000502:physiology*; D051876:Sex-Determining Region Y Protein; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; C054430:ZFY protein, human