title: The influence of the organic matrix on demineralization of bovine root dentin in vitro.
abstract: The effect of matrix degradation on the rate of demineralization of dentin lesions was investigated. It was hypothesized that the demineralized matrix would inhibit the demineralization of the underlying mineralized dentin. Bovine root dentin specimens were alternately demineralized and incubated with either a bacterial collagenase or buffer (control). The demineralization was carried out under various conditions: Acetic acid solutions were used to form incipient and advanced erosive lesions, and lactic acid solutions containing a bisphosphonate were used to form incipient subsurface lesions. Under all conditions, the demineralization was found to be accelerated when the matrix was degraded by collagenase. This increase was more pronounced in advanced erosive lesions than in incipient lesions. Microscopic examination of collagenase-treated specimens revealed that the matrix of erosive lesions contained several layers of differently affected matrices, whereas the matrix of subsurface lesions appeared to be equally affected throughout the lesion. In conclusion, the matrix degradation was different in erosive and subsurface lesions but promoted the demineralization in both types of lesions.
authors.affiliation: Department of Cariology and Endodontology, Academic Centre for Dentistry (ACTA), Amsterdam, The Netherlands.
authors.forename: G A
authors.initials: GA
authors.lastname: Kleter
authors.forename: J J
authors.initials: JJ
authors.lastname: Damen
authors.forename: V
authors.initials: V
authors.lastname: Everts
authors.forename: J
authors.initials: J
authors.lastname: Niehof
authors.forename: J M
authors.initials: JM
authors.lastname: Ten Cate
mesh_terms: D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000818:Animals; D002021:Buffers; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002417:Cattle; D017364:Collagenases / Q000378:metabolism; D003804:Dentin / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004164:Diphosphonates; D006909:Hydroxyproline / Q000032:analysis; D007773:Lactates / Q000494:pharmacology; D019344:Lactic Acid; D017213:Root Caries / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D017001:Tooth Demineralization / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D014092:Tooth Root / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
chemical_list: D000085:Acetates; D002021:Buffers; D004164:Diphosphonates; D007773:Lactates; D019344:Lactic Acid; D017364:Collagenases; D019342:Acetic Acid; D006909:Hydroxyproline; D002118:Calcium