Evidence for an intermediate in the enzymatic formation of uroporphyrinogen III.
Evidence for an azafulvene intermediate in the enzymatic formation of Uroporphyrinogen III has been obtained. Using conditions to slow down the enzyme activity (high pH, low temperature), the transient species was trapped with ammonium ions as aminomethylbilane and with sodium borohydride as methylbilane, and observed by 13C-NMR.
Pichon
C
C
Department of Chemistry, Texas A&M University, College Station 77843-3255.
Atshaves
B P
BP
Stolowich
N J
NJ
Scott
A I
AI
D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013696:Temperature; D014576:Uroporphyrinogen III Synthetase / Q000378:metabolism; D014577:Uroporphyrinogens / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D014577:Uroporphyrinogens; C024393:hydroxymethylbilane; D014576:Uroporphyrinogen III Synthetase