Age- and gender-related changes in the hepatic metabolism of 2-methylpropene and relationship to epoxide metabolizing enzymes.
The effect of age and gender on the in vitro biotransformation of 2-methylpropene, an alkene metabolized to 2-methyl-1,2-epoxypropane, was studied. The epoxide concentration and the epoxide metabolizing enzymatic activities were investigated in male and female Brown Norway rats of different ages. Liver tissue of senescent rats was exposed to smaller 2-methyl-1,2-epoxypropane concentrations than that of young animals, although changes during ageing were rather modest. With advancing age a feminization of male glutathione S-transferase and cytosolic epoxide hydrolase activities was found, as well as a significant decline of the female microsomal epoxide hydrolase activity and an increase of the cytochrome P-450 content in the oldest female rats.
Cornet
M
M
Department of Toxicology, Vrije Universiteit Brussel, Belgium.
Mertens
K
K
Callaerts
A
A
Sonck
W
W
Vercruysse
A
A
Rogiers
V
V
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000475:Alkenes / Q000493:pharmacokinetics*; D000818:Animals; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004851:Epoxide Hydrolases / Q000378:metabolism; D004852:Epoxy Compounds / Q000378:metabolism*; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D012727:Sex Characteristics
D000475:Alkenes; D004852:Epoxy Compounds; D003577:Cytochrome P-450 Enzyme System; D015660:7-Alkoxycoumarin O-Dealkylase; D005982:Glutathione Transferase; D004851:Epoxide Hydrolases; C008176:isobutylene