title: A novel carbonic anhydrase II mRNA isolated from mature chicken testis displays a TATA box and other promoter sequences in a leader 5' untranslated region not present in somatic tissues.
abstract: The primary structure of a novel carbonic anhydrase II-encoding cDNA clone (CAII) isolated from a chicken testis cDNA library is presented. The size of the CAII mRNA obtained from meiotic and haploid chicken testis cells is larger than the corresponding mRNA from immature testis and somatic tissues. The nucleotide sequence of the chicken testis CAII clone revealed a protein-coding region identical to the published sequence of CAII mRNA from erythroid cells. However, the 5' untranslated region (UTR) of the testis CAII mRNA is larger than the corresponding somatic sequence. The 5' UTR contains a leader sequence not present in the CAII mRNA isolated from erythroid cells or chick retina. The additional 5' UTR of the mRNA displays a TATA box, located 23-30 bp upstream from the cap site of the CAII mRNA transcribed in erythroid cells, and several G+C-rich boxes. Our results suggest that the use of a testis-specific promoter would result in the incorporation of somatic promoter sequences into the 5' UTR of the testis message.
authors.affiliation: Molecular Genetics Research Group, Faculty of Medicine, University of Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Mezquita
authors.forename: M
authors.initials: M
authors.lastname: Pau
authors.forename: C
authors.initials: C
authors.lastname: Mezquita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002256:Carbonic Anhydrases / Q000235:genetics*; D002645:Chickens; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification*; D016385:TATA Box; D013737:Testis / Q000201:enzymology*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D002256:Carbonic Anhydrases