Potential interaction of interleukin-4 with endogenous cytokines in vivo.
We employed alginate-entrapped cells secreting recombinant murine interleukin-4 (IL-4) to study the effect of IL-4 on hematopoietic cells of normal mice. The most dramatic effect was registered in an increase in the neutrophils and to a lesser extent the monocytes. A small increase in the CD4+, CD8+ and B220+ populations was also observed. Serum IgE levels also increased dramatically. All of these increases could be specifically inhibited with anti-IL-4. Antibodies to granulocyte-macrophage colony-stimulating factor, IL-3 and IL-5 could also inhibit some IL-4-mediated actions suggesting an interplay between these cytokines in vivo.
Sullivan
L M
LM
Cell Biology Department, Schering-Plough Research Institute, Kenilworth, N.J. 07033.
Bober
L A
LA
Grace
M J
MJ
Braun
S
S
Macosko
H D
HD
Payvandi
F
F
Pugliese-Sivo
C C
CC
Narula
S K
SK
D000464:Alginates; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002460:Cell Line; D016207:Cytokines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007073:Immunoglobulin E / Q000097:blood; D015847:Interleukin-4 / Q000276:immunology / Q000494:pharmacology*; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology*; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010529:Peritoneal Cavity / Q000166:cytology; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000464:Alginates; D000906:Antibodies; D016207:Cytokines; D006603:Hexuronic Acids; D011994:Recombinant Proteins; D015847:Interleukin-4; D007073:Immunoglobulin E; D020723:Glucuronic Acid