Cloning of a human cDNA encoding a putative nucleotide-binding protein related to Escherichia coli MinD.
A novel human cDNA encoding a putative nucleotide-binding protein (NBP) was obtained by screening a human SHSY5Y neuroblastoma library. The deduced protein contains 320 amino acids (aa) with a M(r) of 34,540. NBP displays sequence similarity with the product of the minD gene from Escherichia coli. MinD is involved in the proper placement of the division septum, and has ATPase activity. NBP and MinD contain consensus nucleotide (nt)-binding domains. The NBP mRNA is approx. 1500 nt in length and is expressed in several human cell lines and in all rat tissues examined, with the highest levels in lung and testis.
Shahrestanifar
M
M
Department of Biochemistry and Molecular Biology, UMD-New Jersey Medical School, Newark 07103.
Saha
D P
DP
Scala
L A
LA
Basu
A
A
Howells
R D
RD
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D018076:DNA, Complementary; D029968:Escherichia coli Proteins; D047908:Intracellular Signaling Peptides and Proteins; C089765:NUBP1 protein, human; D009711:Nucleotides; D012333:RNA, Messenger; D000251:Adenosine Triphosphatases; C072069:MinD protein, E coli