Role of CD26/dipeptidyl peptidase IV in human immunodeficiency virus type 1 infection and apoptosis.
To examine the role of CD26/dipeptidyl peptidase IV (DPPIV; EC 3.4.14.5) in infection by human immunodeficiency virus type 1 (HIV-1), we utilized CD26 cDNA-transfected Jurkat T-cell lines. Both CD26- parental Jurkat cells and mutant CD26+ (DPPIV-) transfected Jurkat cells were readily infected with HIV-1, whereas wild-type CD26+ (DPPIV+) transfected Jurkat cells were more resistant to HIV-1 infection. Our results suggest that CD26 is not essential for HIV-1 infectivity as suggested by others but that DPPIV enzyme activity may decrease the efficiency of HIV-1 infection. Of great interest, we found that mutant CD26+ (DPPIV-) transfectants and CD26- parental Jurkat cells strongly expressed CD95 (Fas/Apo-1) and were more sensitive than wild-type CD26+ (DPPIV+) transfectants to the induction of apoptosis by anti-CD95 monoclonal antibody. These results suggest that CD26 may play a role in HIV-1-associated loss of -CD4+ cells through the process of programmed cell death.
Morimoto
C
C
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA.
Lord
C I
CI
Zhang
C
C
Duke-Cohan
J S
JS
Letvin
N L
NL
Schlossman
S F
SF
D015703:Antigens, CD / Q000502:physiology*; D017209:Apoptosis / Q000276:immunology*; D001483:Base Sequence; D015704:CD4 Antigens / Q000096:biosynthesis; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis / Q000502:physiology*; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D013698:Templates, Genetic; D014162:Transfection
D015703:Antigens, CD; D015704:CD4 Antigens; D009838:Oligodeoxyribonucleotides; D018819:Dipeptidyl Peptidase 4