Factors that bind to RNA polymerase I promoter sequences of Trypanosoma brucei.
The procyclic acidic repetitive protein (procyclin) and variant surface glycoprotein genes of Trypanosoma brucei are transcribed by a polymerase sharing many features with RNA polymerase I. Mutational analyses on the PARP and ribosomal RNA promoters have shown that sequences important for promoter activity are concentrated 20-60 bp upstream of the transcription initiation site. The results of gel mobility shift assays using synthetic oligonucleotides spanning of these regions indicated the presence in trypanosomal extracts of factors capable of binding each promoter in a highly specific fashion. There was no evidence that the PARP, VSG and rRNA promoter fragments bound the same factor.
Janz
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L
Zentrum für Molekulare Biologie, Heidelberg, Germany.
Hug
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Clayton
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C
D000818:Animals; D001483:Base Sequence; D018105:DNA, Kinetoplast / Q000235:genetics / Q000378:metabolism; D016054:DNA, Protozoan / Q000235:genetics / Q000378:metabolism; D017125:Genes, Protozoan; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D015800:Protozoan Proteins; D012318:RNA Polymerase I / Q000235:genetics*; D016053:RNA, Protozoan / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics
D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D014643:Variant Surface Glycoproteins, Trypanosoma; C053277:procyclic acidic repetitive protein, Trypanosoma; D012318:RNA Polymerase I