Human recombinant interleukin-1 receptor antagonist blocks bone resorption induced by interleukin-1 beta but not interleukin-1 alpha.
Both interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are powerful stimulators of bone resorption in vivo and in vitro. Interleukin-1 receptor antagonist (IL-1ra) binds to many interleukin-1 receptors. It does not activate the receptor and effectively blocks the action of IL-1 alpha and IL-1 beta. In this study, human recombinant IL-1ra, at 100-fold excess, was found to block bone resorption in cultured mouse calvaria due to IL-1 beta but not IL-1 alpha. These observations may be explained by differential affinities of receptors for IL-1 alpha, IL-1 beta and rhIL-1ra on target bone cells.
Chole
R A
RA
Otolaryngology Research Laboratories, School of Medicine, University of California, Davis.
Tinling
S P
SP
Faddis
B T
BT
D000704:Analysis of Variance; D000818:Animals; D001862:Bone Resorption / Q000139:chemically induced / Q000517:prevention & control*; D002478:Cells, Cultured; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000633:toxicity*; D051379:Mice; D010010:Osteoclasts / Q000187:drug effects*; D010281:Parathyroid Hormone / Q000633:toxicity; D010446:Peptide Fragments / Q000633:toxicity; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D012795:Sialoglycoproteins / Q000494:pharmacology / Q000627:therapeutic use*; D019379:Teriparatide
C507763:IL1RN protein, human; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D019379:Teriparatide