title: Acquired thermotolerance and heat shock proteins in thermophiles from the three phylogenetic domains.
abstract: Thermophilic organisms from each of the three phylogenetic domains (Bacteria, Archaea, and Eucarya) acquired thermotolerance after heat shock. Bacillus caldolyticus grown at 60 degrees C and heat shocked at 69 degrees C for 10 min showed thermotolerance at 74 degrees C, Sulfolobus shibatae grown at 70 degrees C and heat shocked at 88 degrees C for 60 min showed thermotolerance at 95 degrees C, and Thermomyces lanuginosus grown at 50 degrees C and heat shocked at 55 degrees C for 60 min showed thermotolerance at 58 degrees C. Determinations of protein synthesis during heat shock revealed differences in the dominant heat shock proteins for each species. For B. caldolyticus, a 70-kDa protein dominated while for S. shibatae, a 55-kDa protein dominated and for T. lanuginosus, 31- to 33-kDa proteins dominated. Reagents that disrupted normal protein synthesis during heat shock prevented the enhanced thermotolerance.
authors.affiliation: Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, Illinois 60439.
authors.forename: J D
authors.initials: JD
authors.lastname: Trent
authors.forename: M
authors.initials: M
authors.lastname: Gabrielsen
authors.forename: B
authors.initials: B
authors.lastname: Jensen
authors.forename: J
authors.initials: J
authors.lastname: Neuhard
authors.forename: J
authors.initials: J
authors.lastname: Olsen
mesh_terms: D000222:Adaptation, Physiological; D001407:Bacillus / Q000502:physiology*; D003513:Cycloheximide / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D003904:Mitosporic Fungi / Q000502:physiology*; D014176:Protein Biosynthesis / Q000187:drug effects; D016944:Sulfolobus / Q000502:physiology*
chemical_list: D006360:Heat-Shock Proteins; D003513:Cycloheximide