Serum and urine levels of levocarnitine family components in genetically diabetic rats.
Serum concentration and urinary excretion of levocarnitine (L-carnitine, CAS 541-15-1) family components were evaluated in a Wistar derived strain of genetically diabetic rats BB/BB, in comparison with normal Wistar rats, and their control rats BB/WB of both sexes. BB/BB diabetic animals have lower serum concentration of total-L-carnitine (TC), L-carnitine (LC), acetyl-L-carnitine (ALC), and short chain L-carnitine esters (SCLCE) than both the strains of non-diabetic rats, as previously observed in streptozotocin diabetic rats. No or marginal variations between control and diabetic rats were detected in cumulative urinary excretion of L-carnitine family components. A strain difference was observed between Wistar and BB/WB non-diabetic rats, BB/WB showing higher serum concentration and lower cumulative urinary excretion of LC and TC than Wistar animals. Renal clearance of L-carnitine components proved to be markedly higher in BB/BB diabetic rats, as previously shown in streptozotocin rats. The reduction of serum concentration of the carnitines endogenous pool may explain this finding. The lack of an increased urinary excretion of L-carnitine components in diabetic animals despite the high increase of diuresis suggests that the saturable tubular reabsorption of L-carnitine family components also in diabetes is the primary mechanism to preserve the homeostatic equilibria of the L-carnitine family, the variation in serum concentration being attributable to the complex systemic metabolic alterations typical of diabetes. In agreement with previous investigations, male animals of all the strains showed higher serum concentration and urinary excretion of L-carnitine components as compared to females.
Morabito
E
E
Department of Pharmacology, Sigma-Tau S.p.A., Pomezia, Roma, Italy.
Corsico
N
N
Marzo
A
A
Arrigoni Martelli
E
E
D000108:Acetylcarnitine / Q000097:blood / Q000652:urine; D000818:Animals; D001786:Blood Glucose / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D002331:Carnitine / Q000097:blood* / Q000652:urine*; D003920:Diabetes Mellitus / Q000235:genetics* / Q000378:metabolism*; D004231:Diuresis / Q000502:physiology; D005260:Female; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012727:Sex Characteristics; D013045:Species Specificity; D014563:Urodynamics / Q000502:physiology
D001786:Blood Glucose; D000108:Acetylcarnitine; D002331:Carnitine