Isolation of bluetongue virus from sheep in Rajasthan State, India.
A cytopathic agent was isolated in a baby hamster kidney (BHK)-21 cell-line from blood samples of cross-bred sheep showing typical bluetongue symptoms at Avikanagar in Rajasthan State, India. The cytopathic agent was identified as a bluetongue virus (BTV) by immunofluorescence, the immunoperoxidase test and electron microscopy of BHK-21 cells infected with the new isolate. The new isolate was typed as BTV serotype 1.
Prasad
G
G
Department of Veterinary Microbiology, College of Veterinary Sciences, Haryana Agricultural University, Hisar, India.
Garg
A K
AK
Minakshi
Kakker
N K
NK
Srivastava
R N
RN
D000818:Animals; D001819:Bluetongue / Q000821:virology*; D001820:Bluetongue virus / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D002460:Cell Line; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D007194:India; D008854:Microscopy, Electron; D012703:Serotyping / Q000662:veterinary; D012756:Sheep