PAF acetylhydrolase and arachidonic acid metabolite levels in patients with sepsis.
To examine the relationship between arachidonic acid metabolism and sepsis, we determined the blood concentration of platelet-activating factor acetylhydrolase (PAFAH) and of arachidonic acid cascade substances, leukotriene B4 (LTB4), thromboxane B2 (TXB2), and 6-keto-prostaglandin Fla (PGF1a), in patients with clinical sepsis. Blood concentrations of PAFAH, LTB4, TXB2 and PGF1a at the time of the initial diagnosis of sepsis were 23.9 +/- 9.7 nmol/min/mL, 90.4 +/- 31.5 pg/mL, 55.3 +/- 44.1 pg/mL and 22.3 +/- 11.9 pg/mL, respectively. There was a significant correlation between the blood PAFAH and LTB4 concentration (r = 0.520, p = 0.0033) and the diagnosis of sepsis. There also was a significant correlation between the blood PAFAH and TXB2 concentration (r = 0.460, p = 0.0311). There was no correlation between the blood PAFAH and PGF1a concentrations. Elevated LTB4 and TXB2 concentrations showed a significant correlation with the PAFAH concentration in patients with sepsis, suggesting a direct relationship between these substances.
Takakuwa
T
T
Critical Care and Emergency Center, Iwate Medical University, Morioka, Japan.
Endo
S
S
Nakae
H
H
Kikichi
M
M
Inada
K
K
Yoshida
M
M
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001095:Arachidonic Acids / Q000097:blood*; D005260:Female; D006801:Humans; D007975:Leukotriene B4 / Q000097:blood; D008297:Male; D008875:Middle Aged; D010741:Phospholipases A / Q000097:blood*; D011863:Radioimmunoassay; D018805:Sepsis / Q000097:blood*; D013929:Thromboxane B2 / Q000097:blood
D001095:Arachidonic Acids; D007975:Leukotriene B4; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase