title: Purification and spectroscopic characterization of a recombinant amino-terminal polypeptide fragment of mouse epithelial cadherin.
abstract: Cadherins are a family of Ca(2+)-dependent cell adhesion molecules containing four extracellular tandem repeats each of 110 amino acids. The most amino-terminal repeat is believed to confer the specificity of cell adhesion. A polypeptide containing the amino-terminal repeat of mouse epithelial cadherin has been over-expressed in E. coli and purified to homogeneity. This polypeptide binds Ca2+ with a dissociation constant of 1.6 x 10(-4) M. CD and NMR experiments indicate that the polypeptide adopts a predominantly beta-sheet conformation and that binding of Ca2+ induces only small conformational changes.
authors.affiliation: Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Canada.
authors.forename: K I
authors.initials: KI
authors.lastname: Tong
authors.forename: P
authors.initials: P
authors.lastname: Yau
authors.forename: M
authors.initials: M
authors.lastname: Overduin
authors.forename: S
authors.initials: S
authors.lastname: Bagby
authors.forename: T
authors.initials: T
authors.lastname: Porumb
authors.forename: M
authors.initials: M
authors.lastname: Takeichi
authors.forename: M
authors.initials: M
authors.lastname: Ikura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015820:Cadherins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004926:Escherichia coli; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping
chemical_list: D015820:Cadherins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011994:Recombinant Proteins; D002118:Calcium