The regulation of sodium-dependent transport of anionic amino acids in cultured human fibroblasts.
In cultured human fibroblasts the transport of anionic amino acids through the sodium-dependent system X-AG is stimulated rapidly and transiently by phorbol 12,13-dibutyrate. Transport stimulation is consistent with an effect due to the activation of protein kinase C. Bradykinin (1 microM) and PDGF-AA (100 ng/ml) also stimulate the activity of system X-AG. The bradykinin effect appears to be fully dependent upon PKC activation whereas the stimulation of aspartate transport by PDGF-AA is also due to PKC-independent mechanisms.
Franchi-Gazzola
R
R
Istituto di Patologia Generale, Università degli Studi di Parma, Italy.
Visigalli
R
R
Bussolati
O
O
Gazzola
G C
GC
D000293:Adolescent; D000470:Alkaloids / Q000494:pharmacology; D001120:Arginine / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism*; D000077214:Becaplermin; D001692:Biological Transport / Q000187:drug effects; D001920:Bradykinin / Q000378:metabolism / Q000494:pharmacology; D002478:Cells, Cultured; D015536:Down-Regulation; D004789:Enzyme Activation; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D012867:Skin / Q000166:cytology; D012964:Sodium / Q000378:metabolism; D019311:Staurosporine
D000470:Alkaloids; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; C075278:platelet-derived growth factor A; D000077214:Becaplermin; D001224:Aspartic Acid; D015240:Phorbol 12,13-Dibutyrate; D001120:Arginine; D012964:Sodium; D011493:Protein Kinase C; D019311:Staurosporine; D001920:Bradykinin