title: Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep.
abstract: This paper reports the development and evaluation of serological tests for the differentiation of antibodies in animals infected with capripox and parapox viruses. Agar-gel immunodiffusion tests using sera from sheep with naturally-acquired infections and from sheep experimentally inoculated with orf or capripox viruses showed cross reactions. Virus-specific antibody responses to structural proteins of the viruses were analysed by Western-blot analysis. This analysis readily differentiated the infections as either capripox or contagious pustular dermatitis. The antibody responses to the 32 kDa and 26 kDa proteins of capripoxvirus provided a firm basis for differentiation.
authors.affiliation: Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, England.
authors.forename: P
authors.initials: P
authors.lastname: Chand
authors.forename: R P
authors.initials: RP
authors.lastname: Kitching
authors.forename: D N
authors.initials: DN
authors.lastname: Black
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000302:isolation & purification*; D015153:Blotting, Western; D018151:Capripoxvirus / Q000276:immunology*; D003937:Diagnosis, Differential; D004474:Ecthyma, Contagious / Q000175:diagnosis / Q000276:immunology*; D005779:Immunodiffusion; D011213:Poxviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral