title: Action of a diffusible target-derived chemoattractant on cortical axon branch induction and directed growth.
abstract: Cortical axons innervate their brainstem target, the basilar pons, by the initiation and extension of collateral branches interstitially along their length. To address whether a diffusible pons-derived chemoattractant controls these events, we used cocultures in collagen matrices and time-lapse microscopy. Pontine explants enhanced by 5-fold the de novo initiation of transient branches along cortical axons; most branches were directed toward pons. Of the branches extended toward pons, 2%-3% were stabilized; those extended away were not. Pontine explants also enhanced the stable bifurcation of growth cones and prompted directional changes by growth cone turning and collateral extension. These effects were distance dependent and mimicked by pons-conditioned medium. This evidence indicates that the pons activity promotes branch initiation interstitially along cortical axons, a novel property for a chemoattractant, and provides a directional cue for their growth. These findings suggest that the pons chemoattractant serves as a diffusible target-recognition molecule.
authors.affiliation: Molecular Neurobiology Laboratory, Salk Institute, La Jolla, California 92037.
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: L
authors.initials: L
authors.lastname: Lopez-Mascaraque
authors.forename: C D
authors.initials: CD
authors.lastname: Heffner
authors.forename: D D
authors.initials: DD
authors.lastname: O'Leary
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000648:ultrastructure*; D002630:Chemotactic Factors / Q000502:physiology*; D003094:Collagen; D003470:Culture Media; D017077:Culture Media, Conditioned; D046508:Culture Techniques; D008853:Microscopy; D011149:Pons / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014741:Video Recording
chemical_list: D002630:Chemotactic Factors; D003470:Culture Media; D017077:Culture Media, Conditioned; D003094:Collagen