title: Immunological quantification of advanced glycosylation end-products in the serum of patients on hemodialysis or CAPD.
abstract: We have developed an immunological procedure for measuring advanced glycosylation end-products (AGEs) in serum. Using this method, we measured AGEs in healthy volunteers, patients with diabetes, renal failure without treatment and in patients with renal failure, treated with hemodialysis (HD) or continuous ambulatory peritoneal dialysis (CAPD). We found that AGEs were moderately elevated in diabetics without renal failure and highly elevated in CAPD and HD patients irrespective of their glycemic status. AGE levels correlated significantly with creatinine levels but not with levels of glucose or patient age or sex. AGE levels were reduced significantly post-hemodialysis. Preliminary experiments have shown that circulating AGEs have a molecular weight of approximately 1.5 to 2.0 kDa. More studies are needed to establish if AGE measurements in serum are prognostic indicators of the complications of either diabetes or renal failure.
authors.affiliation: Department of Medicine, Toronto Hospital, Toronto Western Division, Ontario, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Papanastasiou
authors.forename: L
authors.initials: L
authors.lastname: Grass
authors.forename: H
authors.initials: H
authors.lastname: Rodela
authors.forename: A
authors.initials: A
authors.lastname: Patrikarea
authors.forename: D
authors.initials: D
authors.lastname: Oreopoulos
authors.forename: E P
authors.initials: EP
authors.lastname: Diamandis
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003404:Creatinine / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000628:therapy; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000628:therapy; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood*; D006031:Glycosylation; D006801:Humans; D007124:Immunoenzyme Techniques; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D008875:Middle Aged; D008970:Molecular Weight; D010531:Peritoneal Dialysis, Continuous Ambulatory; D011817:Rabbits; D006435:Renal Dialysis
chemical_list: D001786:Blood Glucose; D017127:Glycation End Products, Advanced; D003404:Creatinine