title: Comparative 3H-CCK-8S binding studies on CCKB-type receptors in guinea-pig cortex and continuous cell lines.
abstract: The present study was undertaken to compare binding characteristics of CCKB-type receptors in guinea-pig cortex, Jurkat T-cells, GH3 cells and C6 cells. The rank order of potency of a variety of CCK agonists and antagonists in inhibiting specific [3H]CCK-8S binding was highly correlated for the 4 CCKB receptor models as demonstrated by a computer-assisted statistical analysis. Taking the ligand binding profiles as the criterion it is concluded that CCKB receptors in guinea-pig cortex, Jurkat T-cells, pituitary GH3 cells and rat glioma C6 cells share identical pharmacological properties.
authors.affiliation: Institute of Pharmacology and Toxicology, Medical Faculty (Charité), Humboldt University of Berlin, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Kaufmann
authors.forename: T
authors.initials: T
authors.lastname: Schöneberg
authors.forename: P
authors.initials: P
authors.lastname: Henklein
authors.forename: M
authors.initials: M
authors.lastname: Boomgaarden
authors.forename: D
authors.initials: D
authors.lastname: Sohr
authors.forename: T
authors.initials: T
authors.lastname: Ott
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D002540:Cerebral Cortex / Q000378:metabolism*; D002766:Cholecystokinin / Q000819:agonists*; D005910:Glioma; D006168:Guinea Pigs; D007700:Kinetics; D010903:Pituitary Gland, Anterior; D051381:Rats; D011949:Receptors, Cholecystokinin / Q000378:metabolism*; D012844:Sincalide / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014316:Tritium; D014407:Tumor Cells, Cultured
chemical_list: D011949:Receptors, Cholecystokinin; D014316:Tritium; D002766:Cholecystokinin; D012844:Sincalide