title: Immunocytochemical localization of the GABAA receptor in the cerebral cortex of the lizard Psammodromus algirus.
abstract: This study examined the distribution and localization of the gamma-aminobutyric acid (GABA)A receptor in the brain cortex of a reptile by light and electron microscopy, to test whether cortical GABA inhibition is mainly mediated through the GABAA receptor complex. We used preembedding immunocytochemistry and a monoclonal antibody, raised against the receptor complex, that recognizes the beta 2 and beta 3 subunits of the receptor. GABAA receptors were distributed throughout the entire cerebral cortex except the dorsomedial cortex. The immunostaining consisted of fine granules restricted to the plexiform layers of the cortex as seen by light microscopy. This granular aspect of the immunoreactivity most likely corresponds to the immunopositive dendritic and axonal profiles observed under the electron microscope. Some neurons in the medial and lateral cortices displayed patches of immunoreactivity along the cell body and processes, and as a result their morphology was outlined. We discuss the possibility that these neurons were GABAergic as well. The immunocytochemical data demonstrate that the distribution and localization of GABAA receptors in discrete regions of the reptilian cerebral cortex resemble that of parts of the hippocampal formation of humans and rats, suggesting that the basic configuration of the GABA system in these regions is conserved.
authors.affiliation: Department of Cell Biology, Faculty of Sciences, University of Málaga, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Guirado
authors.forename: J C
authors.initials: JC
authors.lastname: Dávila
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001931:Brain Mapping; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008116:Lizards / Q000033:anatomy & histology / Q000378:metabolism*; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D011963:Receptors, GABA-A