The recovery efficiency of various materials for sampling enzymes and polymorphonuclear leukocytes from gingival crevices.
The present investigation was designed to assess the effects of strips made of different materials on the recovery of enzymes in gingival crevicular fluid (GCF) (Experiment 1), and to examine a possible relationship between lysosomal enzyme activities and number of crevicular polymorphonuclear leukocytes (PMNs) (Experiment 2). In Experiment 1, GCF was collected with capillaries from 14 patients with periodontal disease, and applied on various test strips in microcentrifuged tubes or directly into tubes (controls). Strips were then shaken and centrifuged to elute GCF enzymes. The supernate was used for determinations of alkaline phosphatase (ALP), beta-glucuronidase (BG) and aspartate aminotransferase (AST) activities. The % recoveries were calculated as relative percentages to controls. When using saline as eluent, 90% or more ALP, BG and AST were found to be recovered from strips of durapore and papers. More than 35% of ALP and BG was found to remain on paper points. However, the % recoveries from paper points were improved using eluent with 0.1% (w/v) cetyl pyridinium chloride. In Experiment 2, 54 GCF samples were collected from 3 periodontitis patients by using durapore strips, in order to measure both PMN numbers and BG activities in the same samples. The 2 parameters showed strong and positive correlation with 0.847 (p < 0.001) of the Pearson's correlation coefficient. These findings suggest that durapore is a useful material not only for counting PMNs but also for measuring activities of GCF enzymes. Elevation of BG activities in GCF can be due to increasing numbers of PMNs.
Nakashima
K
K
Division of Physiopathology and Periodontology, School of Dentistry, Medical Faculty, University of Geneva, Switzerland.
Demeurisse
C
C
Cimasoni
G
G
D000469:Alkaline Phosphatase / Q000032:analysis; D001219:Aspartate Aminotransferases / Q000032:analysis; D004526:Efficiency; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D005966:Glucuronidase / Q000032:analysis; D006801:Humans; D007958:Leukocyte Count; D008247:Lysosomes / Q000201:enzymology*; D008851:Micropore Filters; D009504:Neutrophils / Q000473:pathology*; D010209:Paper; D010518:Periodontitis / Q000201:enzymology / Q000473:pathology; D011934:Reagent Strips; D012965:Sodium Chloride
D011934:Reagent Strips; D012965:Sodium Chloride; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase; D005966:Glucuronidase