The trace analysis of musk xylene in biological samples: problems associated with its ubiquitous occurrence.
Musk xylene (1-tert-butyl-3,5-dimethyl-2,4,6-trinitrobenzene) (MX), a synthetic musk often used in fragrances has previously been described to occur in human tissue. In this article a specific and sensitive method for the determination of MX in blood is described. It includes a simple clean-up by silica gel adsorption chromatography followed by GC/MS detection with negative chemical ionisation (NCI) and multiple ion detection of the molecular ion and M-30. The absolute detection limit in the MID-mode was 50 fg of MX. 11 human blood samples of 3 individuals were analysed to elucidate the suitability of this method. The MX concentrations ranged from 66 to 270 pg/g plasma or 12 to 49 ng/g blood lipids, respectively. In the course of the method evaluation the hazard of sample contamination during the clean-up procedure was investigated and MX was found to be present in several materials in the laboratory. Some of these contamination sources could be eliminated.
Helbling
K S
KS
Institute of Toxicology, Swiss Federal Institute of Technology, Schwerzenbach.
Schmid
P
P
Schlatter
C
C
D000328:Adult; D004866:Equipment Contamination; D005260:Female; D006801:Humans; D007753:Laboratories; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D015203:Reproducibility of Results; D014992:Xylenes / Q000097:blood*
D014992:Xylenes; C031447:musk xylene