Stimulation of calcium sequestration by mezerein, a protein kinase C activator, in saponized rabbit platelets.
1. The saponin-permeabilized platelet was used to examine the effect of mezerein, a moderate activator of protein kinase C (C-kinase), on the sequestration of Ca2+ to its intracellular storage sites. 2. We found that the activation of C-kinase by mezerein causes the potentiation of the Ca2+ sequestration. 3. It was suggested that C-kinase in platelets might function as a negative feedback regulator for the agonist-induced Ca2+ mobilization and might be involved in its oscillation.
Nishio
H
H
Department of Pharmacology, Hiroshima University School of Medicine, Japan.
Ikegami
Y
Y
Segawa
T
T
Nakata
Y
Y
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000097:blood*; D004224:Diterpenes; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D011493:Protein Kinase C / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits; D012503:Saponins / Q000008:administration & dosage; D013268:Stimulation, Chemical; D013729:Terpenes / Q000494:pharmacology*
D004224:Diterpenes; D012503:Saponins; D013729:Terpenes; C011309:mezerein; D011493:Protein Kinase C; D002118:Calcium