DNA of lymphoma-associated herpesvirus (HVMF1) in SIV-infected monkeys (Macaca fascicularis) shows homologies to EBNA-1, -2 and -5 genes.
We have characterized a new Epstein-Barr-virus(EBV)-like herpesvirus associated with lymphomas of SIV-infected cynomolgus (Macaca fascicularis) monkeys and propose that this virus is designated herpesvirus macaca fascicularis I (HVMFI). Genomic regions in HVMF1 of potential significance for tumor pathogenesis were analyzed by Southern blotting, PCR and sequencing, and compared with human EBV DNA. Virus from 7 SIV-associated lymphomas and one lymphoma-derived cell line were shown to share homology with the EBNA1- and EBNA2-coding regions of EBV, while some homology to EBV-LMP1 was detectable only at low-stringency hybridization. Homologous regions to the long internal repeat (IR1; BamHI W), the EBER1 and 2 and the latent origin of DNA replication (oriP) could also be demonstrated in HVMF1. These coding regions, except IR1, showed restriction-enzyme maps different from those of EBV. Sequencing of the EBNA5 homologous region of HVMF1 DNA, corresponding to exons W1 and W2, showed 65% homology to the EBV exons W1 and W2, and 80% to the whole region including the intron. Since EBNA5 has been reported to bind tumor-suppressor proteins p53 and Rb in vitro, the HVMF1 homology could be important for the lymphomagenic capacity of this monkey herpesvirus.
Li
S L
SL
Immunopathology Laboratory, Karolinska Institute, Stockholm, Sweden.
Biberfeld
P
P
Ernberg
I
I
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D019309:Epstein-Barr Virus Nuclear Antigens; D005091:Exons; D015497:HIV-1; D015498:HIV-2; D006564:Herpesviridae / Q000737:chemistry / Q000235:genetics*; D006566:Herpesviridae Infections / Q000150:complications* / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics; D008223:Lymphoma / Q000150:complications* / Q000821:virology*; D016483:Lymphoma, AIDS-Related / Q000821:virology; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D016601:RNA-Binding Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000821:virology*; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000235:genetics
D000956:Antigens, Viral; D004273:DNA, Neoplasm; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D016601:RNA-Binding Proteins; D014763:Viral Matrix Proteins