Hematopoietic progenitor cells are resistant to dimethyl sulfoxide toxicity.
BACKGROUND
A direct chemical toxicity of dimethyl sulfoxide (DMSO) to hematopoietic progenitor cells has been suggested. However, a recent study failed to corroborate these earlier findings. Thus, a series of experiments was undertaken to address this issue.


METHODS
Bone marrow was collected from 18 donors and cryopreserved with 10 percent (vol/vol) DMSO. Aliquots of frozen bone marrow were thawed, diluted with ACD-A to 8 percent (vol/vol) DMSO, and allowed to remain in DMSO for up to 2 hours before mononuclear cells were plated for colony-forming assays. After 14 days in culture, burst-forming units-erythroid, colony-forming units--granulocyte/macrophage, and colony-forming units--granulocyte/erythrocyte/macrophage/megakaryocyte colonies were enumerated.


RESULTS
There was no significant difference (p > 0.5) seen in colony formation over the 2-hour exposure to DMSO.


CONCLUSIONS
These results support and extend a previous study that bone marrow hematopoietic progenitor cells, including burst-forming units--erythroid, colony-forming units--granulocyte/macrophage, and colony-forming units--granulocyte/erythrocyte/macrophage/megakaryocyte are resistant to any toxic effects of 8- to 10-percent (vol/vol) DMSO during at least 2 hours of DMSO exposure.
Branch
D R
DR
Canadian Red Cross Society Blood Services, Toronto, Ontario.
Calderwood
S
S
Cecutti
M A
MA
Herst
R
R
Solh
H
H
D001854:Bone Marrow Cells; D002470:Cell Survival; D004121:Dimethyl Sulfoxide / Q000633:toxicity*; D004351:Drug Resistance; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D013234:Stem Cells / Q000187:drug effects
D004121:Dimethyl Sulfoxide