Biochemical and morphological changes in the lenses of selenium and/or vitamin E deficient rats.
The activities of glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) and free radicals were measured, and the morphological changes were observed in the lens of control rats, selenium-deficient (SeD) and/or vitamin E deficient (VED) rats. The activities of GSH-Px in the lens of SeD rats decreased significantly. The GSH-Px activities of lens were positively related to erythrocytes selenium level. There was a free radical at g = 2.0015 in the rat lens of all groups, but the content of free radicals in the lens of SeD group was significantly higher than that of the control group. The free radical content of lens was negatively related to erythrocytes selenium level, as well as the GSH-Px activities in the lens. In vitro, ultraviolet radiation caused the generation of another kind of free radical (g = 2.0097) in the lens of all groups, but the amount of the free radical in the lens of the SeD group was also significantly higher than that of the control group. The activities of SOD and GSSG-R in VED rat lens were significantly decreased. The amount of MDA in the lens of SeD and/or VED rats were significantly increased. The results showed that the decrease of antioxidative capability in the lenses of SeD and/or VED rats accelerated the lipid peroxidation and generation of free radicals. Although only early morphological changes in SeD and/or VED rat lens were observed, it is considered that selenium and vitamin E deficiency may be involved in the occurrence of cataract.
Cai
Q Y
QY
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing.
Chen
X S
XS
Zhu
L Z
LZ
Xue
A N
AN
Li
W X
WX
Wang
S Q
SQ
Piao
J H
JH
Li
J
J
Sun
C P
CP
Wu
K
K
D000818:Animals; D002386:Cataract / Q000209:etiology; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D007908:Lens, Crystalline / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000172:deficiency*; D014811:Vitamin E Deficiency / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology
D005609:Free Radicals; D012643:Selenium