Reduced serotonergic immunoreactive fibers in the forebrain of alcohol-preferring rats.
Our previous study indicated that 5-hydroxytryptamine (5-HT) immunoreactive fiber densities were decreased in specific areas of the brain in alcohol-preferring rats (P) when compared with alcohol-nonpreferring rats (NP). The results of our current study show that there are quantitative and qualitative differences in 5-HT innervation in other selected regions of the forebrains of P rats. The 5-HT fiber density in the brains of young adult P and NP rats was measured by immunocytochemistry and quantitative image analysis. A routine error of two-dimensional quantitation of nerve fiber was addressed and an adjustment was made. The amount of 5-HT fibers was significantly lower in CA4 and fasciola cinereum of the dorsal hippocampus, caudate-putamen, and hypothalamus of the P as compared with NP rats (unpaired Student's t tests). In examining the fiber types, we found that, in the frontal cortical and hippocampal regions, where normally fine 5-HT fibers with small varicosities and thick 5-HT fibers with large varicosities coexist, fewer fine 5-HT fibers were seen in P rats as compared with NP rats. The fine fibers are known to be vulnerable to abusive drugs. These observations indicate that (a) there are quantitative differences in 5-HT innervation or that the 5-HT in some 5-HT fibers is reduced to a level undetectable by immunocytochemistry, and (b) the fine 5-HT fibers are specifically reduced to a greater degree in the selected brain regions of P rats when compared with that of NP rats. The involvement of the 5-HT system in the alcohol abuse is discussed.
Zhou
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FC
Department of Anatomy, Indiana University School of Medicine, Indianapolis 45202.
Bledsoe
S
S
Lumeng
L
L
Li
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TK
D000428:Alcohol Drinking / Q000473:pathology / Q000503:physiopathology*; D000437:Alcoholism / Q000473:pathology / Q000503:physiopathology*; D000818:Animals; D001931:Brain Mapping; D002421:Caudate Nucleus / Q000473:pathology / Q000503:physiopathology; D005260:Female; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology; D007031:Hypothalamus / Q000473:pathology / Q000503:physiopathology; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D009412:Nerve Fibers / Q000473:pathology / Q000502:physiology; D016548:Prosencephalon / Q000473:pathology / Q000503:physiopathology*; D011699:Putamen / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000502:physiology*
D012701:Serotonin