Human type 2 angiotensin II receptor gene: cloned, mapped to the X chromosome, and its mRNA is expressed in the human lung.
The human type 2 angiotensin II receptor (AT2R) gene was isolated from a human genomic DNA library of adult female leukocytes. The putative coding region of the human AT2R gene is intronless. The primary structure of the human AT2R deduced from the nucleotide sequence of this putative coding region contains 363 amino acid residues and is highly homologous to the sequences of the rat and mouse AT2R. The human AT2R gene was mapped to the X chromosome using chromosomal assignment analysis with a human/rodent somatic cell hybrid panel. Northern blot analysis demonstrated a 3.0 kb mRNA in the human fetal lung and kidney and the human adult lung. These data and probes should enhance research in human AT2R biology.
Koike
G
G
Falk Cardiovascular Research Center, Stanford University School of Medicine, CA 94305-5246.
Horiuchi
M
M
Yamada
T
T
Szpirer
C
C
Jacob
H J
HJ
Dzau
V J
VJ
D000328:Adult; D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002522:Chlorocebus aethiops; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers; D005260:Female; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011945:Receptors, Angiotensin / Q000096:biosynthesis* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014960:X Chromosome
D017931:DNA Primers; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II