Attempts to stabilize a monoclonal antibody with water soluble synthetic polymers of varying hydrophobicity.
Proteins are subject to a variety of physical and chemical reactions that lead to a loss of activity. These reactions are a particular problem in controlled-release devices, where temperatures and protein concentrations are high. Current approaches to increasing protein stability include the addition of saccharides, amino acids, or polymers. New synthetic polymers may be promising protein stabilizers because properties such as molecular weight and side-chain composition can be controlled. In this study, the stability of a murine monoclonal antibody, BR96, was evaluated in solution at 37 degrees C. The antibody was incubated in the presence of a series of synthetic polymers that included poly(glucosylethyl methacrylate) (GEMA) and copolymers of N-vinylpyrrolidone (NVP) and methyl methacrylate (MMA). Samples were taken periodically up to 30 days. The formation of precipitated antibody in particulate aggregates was measured with a Coulter counter, and the molecular-weight distribution of soluble antibody was measured by size-exclusion chromatography. Two trends were evident. First, with poly(GEMA) and copolymers of NVP and MMA, protein aggregation increased at higher polymer concentrations. Second, higher molecular weights of the poly(NVP) homopolymer also led to increases in protein aggregation. Effects of polymer hydrophobicity were more complex. A copolymer containing 9 mol% MMA caused immediate protein precipitation, while a copolymer containing 21 mol% MMA did not. The effects of the copolymer containing 21% MMA were strongly concentration dependent. At 1 wt%, the polymer reduced aggregation, but aggregation increased strongly between concentrations of 2 and 3 wt%.
Antonsen
K P
KP
Center for Bioengineering, University of Washington, Seattle 98195.
Gombotz
W R
WR
Hoffman
A S
AS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D004355:Drug Stability; D005029:Ethylenediamines; D005960:Glucosides / Q000737:chemistry; D008768:Methylmethacrylates / Q000737:chemistry; D051379:Mice; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry; D011205:Povidone / Q000737:chemistry; D011489:Protein Denaturation; D012995:Solubility; D014867:Water
D000911:Antibodies, Monoclonal; D005029:Ethylenediamines; D005960:Glucosides; D008768:Methylmethacrylates; D011108:Polymers; D014867:Water; C005798:N,N,N',N'-tetramethylethylenediamine; C090633:poly(glucosylethyl methacrylate); C037334:poly(methylmethacrylate-N-vinylpyrrolidone); D011205:Povidone