[The cloning and expression of the gene for Staphylococcus aureus beta-hemolysin].
The gene coding the synthesis of beta-hemolysin (phospholipase C) has been cloned from S.aureus strain 126/89. A highly active Escherichia coli producer has been obtained, its capacity of synthesizing phospholipase C exceeding that of the natural strain 37-fold. Phospholipase C (beta-hemolysin) obtained from the natural and gene-engineering producers have been found to be identical in their enzymatic and molecular characteristics. The nucleotide sequence of the full plc gene which codes peptide consisting of 333 acid radicals has been established. In S.aureus strains of different origin this gene has been found to have a conservative character.
Katerov
V E
VE
Golubkov
V I
VI
Totolian
A A
AA
Shalen
K
K
Ensen
L
L
Mikula
I
I
Smola
I
I
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D015723:Gene Library; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D010738:Type C Phospholipases / Q000235:genetics
D006460:Hemolysin Proteins; D010738:Type C Phospholipases
Klonirovanie i ékspressia gena beta-gemolizina Staphylococcus aureus.