Plasmodium vivax merozoite surface protein 1 C-terminal recombinant proteins in baculovirus.
Recombinant proteins derived from the Plasmodium vivax merozoite surface protein 1 have been produced in the baculovirus expression system. These proteins correspond approximately to the Plasmodium vivax analogs of the 42-kDa or 19-kDa C-terminal processing products previously described for Plasmodium falciparum. Each was produced in two versions, either as a membrane-bound entity located on the cell surface and probably carrying a glycosylphosphatidylinositol addition, or as a secreted entity lacking a membrane anchor. Many native conformational epitopes appear to be accurately reproduced in these molecules. Both the 42-kDa and 19-kDa analogs can be N-glycosylated in the baculovirus system and the N-glycosylation appears to be necessary for efficient secretion of both the 42-kDa and 19-kDa recombinant proteins.
Longacre
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S
Unité d'Immunoparasitologie, CNRS URA 361, Institut Pasteur, Paris, France.
Mendis
K N
KN
David
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PH
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006031:Glycosylation; D020066:Merozoite Surface Protein 1; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics; D010966:Plasmodium vivax / Q000235:genetics* / Q000276:immunology; D011487:Protein Conformation; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D018411:Spodoptera
D000953:Antigens, Protozoan; D020066:Merozoite Surface Protein 1; D011498:Protein Precursors; D015800:Protozoan Proteins; D011994:Recombinant Proteins