title: Adhesion of Staphylococcus aureus to bovine mammary epithelial cells induced by growth in milk whey.
abstract: Staphylococcus aureus strains isolated from bovine intramammary infection (mastitis) were tested for adhesion to bovine mammary epithelial cells after growth in milk whey or TSB. Bacteria grown in milk whey adhered more efficiently to mammary gland epithelial cells in vitro than the corresponding homologous bacteria grown in TSB. Trypsin treatment of milk whey-grown S. aureus had no effect on their adherence. Whereas, pretreatment with periodate significantly decreased bacterial adherence capacity. Periodate treatment of TSB-grown bacteria had no effect on adhesion to the mammary gland epithelial cells.
authors.affiliation: Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: W
authors.initials: W
authors.lastname: Mamo
authors.forename: G
authors.initials: G
authors.lastname: Fröman
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D002364:Caseins; D002417:Cattle; D015169:Colony Count, Microbial; D004848:Epithelium / Q000382:microbiology; D005260:Female; D008321:Mammary Glands, Animal / Q000166:cytology / Q000382:microbiology*; D008413:Mastitis / Q000382:microbiology; D008892:Milk / Q000382:microbiology*; D010504:Periodic Acid / Q000494:pharmacology; D011492:Protein Hydrolysates; D013211:Staphylococcus aureus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002364:Caseins; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D010504:Periodic Acid; C009288:metaperiodate