Ouabain and digitoxin as modulators of chick embryo cardiomyocyte energy metabolism.
The effects of 0.1 nmol/l ouabain (CAS 630-60-4) and digitoxin (CAS 71-63-6) on oxygen consumption rate (OCR) and the contractility of cultured chick embryo cardiomyocytes were investigated using a perfusion system which allowed microscopic observation during the experiment. contractility was assessed by a novel image analysis procedure. During substrate free perfusion the OCR was increased on application of 0.1 nmol/l ouabain or digitoxin for 60 min, whilst contractility was not affected. Digitoxin (0.1 nmol/l) elicited no change in the OCR in the presence of glucose, pyruvic acid, lactic acid and caprylic acid. Ouabain (0.1 nmol/l) did not affect the OCR or contractility in the presence of glucose, pyruvic acid and lactic acid, however together with caprylic acid the OCR was increased significantly. These results demonstrate a hitherto unknown stimulation of fatty acid utilization by low concentrations of ouabain.
Riehle
M
M
Cinematic Cell Research Group, Johann Wolfgang Goethe University, Frankfurt/Main, Fed. Rep. of Germany.
Bereiter-Hahn
J
J
D000818:Animals; D002210:Caprylates / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D004074:Digitoxin / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D006321:Heart / Q000187:drug effects / Q000196:embryology; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009124:Muscle Proteins / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D010477:Perfusion; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid
D002210:Caprylates; D007773:Lactates; D009124:Muscle Proteins; D011773:Pyruvates; D019344:Lactic Acid; D010042:Ouabain; D019289:Pyruvic Acid; D004074:Digitoxin; D005947:Glucose; C031492:octanoic acid