Profile of energy metabolism in a murine hybridoma: glucose and glutamine utilization.
The antibody-secreting murine hybridoma, CC9C10, was grown in batch culture in a medium containing 20 mM glucose and 2 mM glutamine. After 2 days of exponential growth, the glutamine content of the medium was completely depleted, whereas the glucose content was reduced to 60% of the original concentration. The glucose and glutamine metabolism was analyzed at midexponential phase by use of radioactively labelled substrates. Glycolysis accounted for the metabolism of most of the glucose utilized (> 96%) with flux through the pentose phosphate pathway (3.6%) and the TCA cycle (0.6%) accounting for the remainder. Glutamine was partially oxidised via glutaminolysis to alanine (55%), aspartate (3%), glutamate (4%), lactate (9%), and CO2 (22%). Calculation of the theoretical ATP production from these pathways indicated that glucose could provide 59% and glutamine 41% of the energy requirement of the cells.
Petch
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Department of Microbiology, University of Manitoba, Winnipeg, Canada.
Butler
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M
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology; D002455:Cell Division; D002952:Citric Acid Cycle; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D006019:Glycolysis; D006825:Hybridomas / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010084:Oxidation-Reduction; D010427:Pentose Phosphate Pathway
D005973:Glutamine; D000255:Adenosine Triphosphate; D005947:Glucose