Potential for pre-implantation determination of human platelet antigen type using DNA amplification: a strategy for prevention of allo-immune thrombocytopenia.
To demonstrate that fetal human platelet antigen (HPA1) type can be determined by amplification from picogram quantities of DNA, nested oligonucleotide DNA primers were designed to amplify a portion of the platelet glycoprotein GpIIIa gene which spans the polymorphic HPA site. Specific oligonucleotides were designed to hybridise either to the amplified HPA1a allele or to the HPA1b allele. DNA dilutions were used as a template and HPA1 type, determined by this method from as little as 2 pg of DNA in 6 cases, was compared to direct typing of blood from the same individuals. In each case nested polymerase chain reaction amplified the specific HPA locus from as little as 2 pg of DNA and determination of HPA1 type agreed with typing of blood.
Bennett
P R
PR
Royal Postgraduate Medical School, Institute of Obstetrics and Gynaecology, Queen Charlotte's and Chelsea Hospital, London, UK.
Vaughan
J
J
Handyside
A
A
Fisk
N M
NM
D016824:Antigens, Human Platelet / Q000235:genetics*; D001483:Base Sequence; D001788:Blood Grouping and Crossmatching; D004247:DNA / Q000032:analysis*; D047108:Embryonic Development; D005260:Female; D006801:Humans; D007518:Isoantibodies; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010980:Platelet Membrane Glycoproteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D013921:Thrombocytopenia / Q000276:immunology* / Q000517:prevention & control
D016824:Antigens, Human Platelet; D007518:Isoantibodies; D010980:Platelet Membrane Glycoproteins; D004247:DNA