title: Skin peptide tyrosine-tyrosine, a member of the pancreatic polypeptide family: isolation, structure, synthesis, and endocrine activity.
abstract: Pancreatic polypeptide, peptide tyrosine-tyrosine (PYY), and neuropeptide tyrosine (NPY), three members of a family of structurally related peptides, are mainly expressed in the endocrine pancreas, in endocrine cells of the gut, and in the brain, respectively. In the present study, we have isolated a peptide of the pancreatic polypeptide family from the skin of the South American arboreal frog Phyllomedusa bicolor. The primary structure of the peptide was established as Tyr-Pro-Pro-Lys-Pro-Glu-Ser-Pro-Gly-Glu10-Asp-Ala-Ser-Pro-Glu-Glu- Met-Asn- Lys-Tyr20-Leu-Thr-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu30-Val-Thr- Arg-Gln-Arg-Tyr-NH2 . This unusual peptide, named skin peptide tyrosine-tyrosine (SPYY), exhibits 94% similarity with PYY from the frog Rana ridibunda. A synthetic replicate of SPYY inhibits melanotropin release from perifused frog neurointermediate lobes in very much the same way as NPY. These results demonstrate the occurrence of a PYY-like peptide in frog skin. Our data also suggest the existence of a pituitary-skin regulatory loop in amphibians.
authors.affiliation: Laboratoire de Bioactivation des Peptides, Institut Jacques Monod, Université de Paris 7, France.
authors.forename: A
authors.initials: A
authors.lastname: Mor
authors.forename: N
authors.initials: N
authors.lastname: Chartrel
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
authors.forename: P
authors.initials: P
authors.lastname: Nicolas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura; D001681:Biological Assay; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010191:Pancreatic Polypeptide / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D011895:Rana ridibunda; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000737:chemistry* / Q000378:metabolism; D013552:Swine; D000521:alpha-MSH / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D010455:Peptides; C090047:skin peptide tyrosine-tyrosine; D000521:alpha-MSH; D010191:Pancreatic Polypeptide