[Analysis of type II pneumocyte protection by pneumoplegic solutions. An experimental in vitro study of lung preservation for transplantation].
With the aim of studying the physiopathological mechanism of lung preservation, we have valued the Pneumocytes Type II viability after six hours of incubation at 4 degrees C in extracellular (Ringer Lactate) and intracellular (Collins, Euro-Collins and Belzer) solutions. The cells have been cut off from adult rat's lung using the modified Dobbs' method. Alveolar Type II viability have been valued using two methods: the total protein content in each culture and the metabolic function of the cells using the rate of protein synthesis by means of 35 S methionine uptake assay. The results have demonstrated a significant difference (p < 0.05) using extracellular solution instead of intracellular. Besides, we have observed a better Pneumocytes Type II survival during Belzer's solution incubation comparing with Collins and Euro-Collins (p < 0.05). Pneumocytes type II require for a better preservation a specific solution that must be similar to extracellular fluid and added with substances able to minimize noxious events during preservation.
Spaggiari
L
L
Istituto di Clinica Chirurgica Generale, Toracica e Vascolare, Università degli Studi di Parma.
Alfieri
R
R
Rusca
M
M
Carbognani
P
P
Petronini
P G
PG
Cattelani
L
L
Dell'Abate
P
P
Bobbio
A
A
Solli
P G
PG
Endrizzi
C
C
D000418:Albumins; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D005942:Gluconates; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D008168:Lung / Q000166:cytology*; D016040:Lung Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D000077325:Ringer's Lactate; D012996:Solutions
D000418:Albumins; C071286:Belzer's gluconate-albumin solution; C024102:Collins' solution; C037096:Euro-Collins' solution; D005942:Gluconates; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D000077325:Ringer's Lactate; D012996:Solutions
Analisi della protezione svolta da soluzioni pulmoplegiche su pneumociti di tipo II. Studio sperimentale in vitro per la conservazione del polmone in funzione del trapianto.