Isolation and sequence analysis of a Caenorhabditis elegans cDNA which encodes a 14-3-3 homologue.
We report the cloning of the Caenorhabditis elegans homologue of a 14-3-3 protein-encoding gene which is located within the unc-22 gene cluster on chromosome IV. Sequence analysis reveals that the cDNA-encoded product is 78% identical to both the Drosophila melanogaster and bovine 14-3-3 proteins. Our cDNA hybridizes to at least three major transcripts of 1.5, 1.35 and 0.9 kb, which are all more abundant in fertile hermaphrodites than in those lacking germ cells.
Wang
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Department of Biology and Institute of Molecular Biology, University of Houston, TX 77204-5513.
Shakes
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D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary / Q000302:isolation & purification; D018509:DNA, Helminth / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D009419:Nerve Tissue Proteins; C106207:par-5 protein, C elegans; D014446:Tyrosine 3-Monooxygenase