Glutathione deficiency does not elevate susceptibility of bacteria to the mutagenicity of chlorinated humic acids.
1. Rat liver 9,000 g supernatant protected against the mutagenic effect of chlorinated hydrophilic macromolecular humic acids (CHMA) in Salmonella typhimurium strain TA100. 2. Protection against mutagenicity of CHMA was mediated by glutathione and was partially dependent on glutathione S-transferase activity. 3. In contrast to the above findings, CHMA showed lower mutagenicity in Salmonella typhimurium and Escherichia coli strains of bacteria that are deficient in glutathione compared to their mutagenicity in parental (glutathione-rich) bacterial strains. 4. Glutathione-deficient cells do not provide test systems with elevated sensitivity for the detection of mutagenic chlorinated humic substances.
Ubom
G A
GA
School of Biochemistry, University of Birmingham, Edgbaston, UK.
Chipman
J K
JK
Hayes
M H
MH
D000818:Animals; D001089:Arabinose / Q000235:genetics / Q000378:metabolism; D002712:Chlorides / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005978:Glutathione / Q000502:physiology*; D005982:Glutathione Transferase / Q000378:metabolism; D006812:Humic Substances / Q000633:toxicity*; D008099:Liver / Q000187:drug effects; D008297:Male; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics
D002712:Chlorides; D006812:Humic Substances; D001089:Arabinose; D005982:Glutathione Transferase; D005978:Glutathione