title: Participation of Na+ channels in the response of carotid body chemoreceptor cells to hypoxia.
abstract: The role played by Na+ channels of carotid body (CB) chemoreceptor cells was investigated by studying the effects of tetrodotoxin (TTX) on the release of 3H-labeled catecholamines ([3H]CA) by adult rabbit CBs previously incubated with the precursor [3H]tyrosine. TTX inhibited partially the release of [3H]CA elicited by mild hypoxia (10 or 7% O2) or by depolarizing incubation medium containing 20 or 30 mM KCl, but the response to more intense hypoxia (5 or 2% O2) or to higher KCl concentration (40 or 50 mM) was not significantly affected. The release of [3H]CA elicited by acidic stimuli, either 20% CO2 (pH 6.6) or the protonophore dinitrophenol (100 microM), although comparable in magnitude to that elicited by mild hypoxia, was not modified by TTX. These results provide evidence for the first time that Na+ channels of chemoreceptor cells participate in the transduction of hypoxic stimuli into the neurotransmitter release response of these cells and suggest that Na+ current operates as an amplifying device that enhances the initial cell depolarization mediated by the closure of the O2-sensitive K+ channels. Sympathetic denervation of CBs was followed by a marked reduction in the release of [3H]CA elicited by veratridine or by 20 mM KCl, suggesting that the number of Na+ channels in chemoreceptor cells decreases after denervation.
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Fisiología, Facultad de Medicina, Universidad de Valladolid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Rocher
authors.forename: A
authors.initials: A
authors.lastname: Obeso
authors.forename: M T
authors.initials: MT
authors.lastname: Cachero
authors.forename: B
authors.initials: B
authors.lastname: Herreros
authors.forename: C
authors.initials: C
authors.lastname: González
mesh_terms: D000143:Acids / Q000494:pharmacology; D000818:Animals; D002344:Carotid Body / Q000473:pathology / Q000503:physiopathology*; D002395:Catecholamines / Q000037:antagonists & inhibitors / Q000378:metabolism; D002628:Chemoreceptor Cells / Q000473:pathology / Q000503:physiopathology*; D000860:Hypoxia / Q000473:pathology / Q000503:physiopathology*; D011188:Potassium / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011817:Rabbits; D015222:Sodium Channels / Q000378:metabolism*; D013562:Sympathectomy; D013779:Tetrodotoxin / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
chemical_list: D000143:Acids; D002395:Catecholamines; D015222:Sodium Channels; D013779:Tetrodotoxin; D011189:Potassium Chloride; D014701:Veratridine; D011188:Potassium