[Influence of combined Salvia miltiorrhiza and Ligusticum wallichii on pharmacokinetics of tetramethylpyrazine in rats].
Influence of Salvia miltiorrhiza (SM) and/or Ligusticum wallichii (LW) on pharmacokinetics of tetramethylpyrazine (TMP) was observed in rats. The content of TMP in LW and LW-SM decoctions were 375.8 and 236.2 micrograms/ml respectively. The rat serum components after oral administration of LW were analyzed by modified HPLC. One of 3 compounds was detected, which was identified as TMP by the comparison of the parameters of 4 spectrums (UV, IR, MS and NMR) with those of TMP in literature. Pharmacokinetics of TMP in rats after the oral administration of LW and LW-SM decoction respectively showed that: (1) both of the data fitted adequately to two-compartment open model; (2) Ka, AUC and serum concentration were higher (P < 0.05-0.01) for LW decoction than that for LW-SM decoction, indicating a higher bioavailability. It demonstrated that the absorption of TMP of LW-SM decoction was slower and the bioavailability of TMP of LW-SM decoction reduced. Contents of TMP in LW and LW-SM decoction and serum concentration of TMP were determined by HPLC method.
Huang
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X
Xijing Hospital, Fourth Military Medical University, Xi'an.
Xia
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T
Ren
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P
D000818:Animals; D001682:Biological Availability; D004344:Drug Incompatibility; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D008297:Male; D010936:Plant Extracts; D011719:Pyrazines / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011719:Pyrazines; C017953:tetramethylpyrazine