Characterization of cord blood stem/progenitor cells.
Based on in vitro studies, umbilical-cord blood has been used clinically to engraft the hematopoietic systems of children. To evaluate the capacity of a single collection of cord blood to engraft an adult, it is important to consider not only the number, but also the quality of stem/progenitor cells in cord blood. We demonstrate that cord blood contains multipotential progenitor cells, possibly subsets of stem cells, that have extensive self-renewal capacity, as estimated by their capacity to form numerous secondary colonies of multiple lineages in secondary replates from single multipotential colonies. This replating capacity is enhanced by a factor or factors, perhaps novel, in cord blood plasma.
Broxmeyer
H E
HE
Walther Oncology Center, Indiana University, Indianapolis 46202-5121.
Carow
C E
CE
D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D005312:Fetal Blood / Q000166:cytology*; D006133:Growth Substances / Q000097:blood; D006412:Hematopoietic Stem Cells; D006801:Humans; D007231:Infant, Newborn
D003470:Culture Media; D006133:Growth Substances