title: A novel cationic amphiphile for transfection of mammalian cells.
abstract: We describe here a new cationic amphiphile, N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine (diC14-amidine), which interacts with plasmid DNA and generates hydrophobic stable complexes resistant against DNase I. In partition experiments between two non-miscible phases, DNA was transferred into an organic phase upon complex formation with diC14-amidine-containing vesicles. Finally, vesicles made of a diC14-amidine and phosphatidylethanolamine (PE) (1:1, mol:mol) mixture or pure diC14-amidine were efficient in mediating transfection of adherent (CHO) and suspension (K562) cell lines, using the chloramphenicol acetyltransferase (CAT) gene as reporter.
authors.affiliation: Laboratoire de Chimie Physique des Macromolécules aux Interfaces CP 206/2, Université Libre de Bruxelles, Belgium.
authors.forename: J M
authors.initials: JM
authors.lastname: Ruysschaert
authors.forename: A
authors.initials: A
authors.lastname: el Ouahabi
authors.forename: V
authors.initials: V
authors.lastname: Willeaume
authors.forename: G
authors.initials: G
authors.lastname: Huez
authors.forename: R
authors.initials: R
authors.lastname: Fuks
authors.forename: M
authors.initials: M
authors.lastname: Vandenbranden
authors.forename: P
authors.initials: P
authors.lastname: Di Stefano
mesh_terms: D000578:Amidines; D000818:Animals; D016466:CHO Cells; D002412:Cations; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000096:biosynthesis; D006224:Cricetinae; D004247:DNA / Q000008:administration & dosage* / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D008081:Liposomes; D008322:Mammals; D010957:Plasmids; D013997:Time Factors; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D000578:Amidines; D002412:Cations; D007202:Indicators and Reagents; D008081:Liposomes; C089665:N-t-butyl-n'-tetradecyl-3-tetradecylaminopropionamidine; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase