Cloning of thyroid hormone receptor genes expressed in metamorphosing flounder.
Two distinct cDNAs encoding thyroid hormone receptors (THRs) were cloned from a lambda gt10 library prepared from the whole bodies of metamorphosing flounder larvae (Paralichthys olivaceus). Deduced amino acid sequences of the two isolated cDNAs shared 96% and 92% homologies in their DNA- and hormone-binding domains, respectively. These were highly conserved when compared to THRs for other vertebrates: 88-96% in the DNA-binding domain and 84-94% in the hormone-binding domain. Other receptors in the nuclear receptor family showed lower homologies than those of THRs. Both THRs for the flounder had higher homologies with the alpha-type THRs of other vertebrates than with the beta-type. Thus, the two THRs for flounder were designated as fTHR alpha A and fTHR alpha B.
Yamano
K
K
National Research Institute of Aquaculture, Mie, Japan.
Araki
K
K
Sekikawa
K
K
Inui
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics*; D029941:Fish Proteins; D005432:Flounder / Q000235:genetics*; D015723:Gene Library; D007814:Larva; D008675:Metamorphosis, Biological / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011485:Protein Binding; D011988:Receptors, Thyroid Hormone / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D016335:Zinc Fingers
D017931:DNA Primers; D004268:DNA-Binding Proteins; D029941:Fish Proteins; D011988:Receptors, Thyroid Hormone; C089573:thyroid hormone receptor alpha A, flounder; C089574:thyroid hormone receptor alpha B, flounder