A Rhizobium tropici DNA region carrying the amino-terminal half of a nodD gene and a nod-box-like sequence confers host-range extension.
Rhizobium tropici CIAT899 is a broad-host-range strain that, in addition to Phaseolus, nodulates other plant legumes such as Leucaena and Macroptilium. The narrow-host-range of Rhizobium leguminosarum biovars phaseoli (strain CE3) and trifolii (strain RS1051) can be extended to Leucaena esculenta and Phaseolus vulgaris plants, respectively, by the introduction of a DNA fragment 521 bp long, which carries 128 amino acids of the amino-terminal region of a nodD gene from R. tropici, as well as a putative nod-box-like sequence, divergently oriented. The 521 bp fragment, in the presence of L. esculenta or P. vulgaris root exudates, induced a R. leguminosarum bv. viciae nodA-lacZ fusion in either a CE3 or RS1051 background, respectively.
Sousa
C
C
Departamento de Microbiología y Parasitologia, Universidad de Sevilla, Spain.
Folch
J L
JL
Boloix
P
P
Megías
M
M
Nava
N
N
Quinto
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli; D007887:Fabaceae / Q000382:microbiology*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000382:microbiology; D010946:Plants, Medicinal; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics*
D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins