[Direct detection of an antigen in immunoglobulin G Langmuir-Blodgett films based on a surface plasma resonance method and in a piezoelectric system].
The possibilities of direct antigen detection in unlabelled systems based on immunoglobulin G Langmuir-Blodgett films as a sensitive surface have been studied. It was shown that an increase in the monolayer number in an immunoglobulin G Langmuir-Blodgett film deposited onto a solid surface coated with a thin silver film (50 nm) resulted in the regeneration of the antigen-binding capacity of the upper antibody layer. This dependence can be used for the construction of a direct optical immunosensor based on surface plasmon resonance. Moreover, a model of a piesoelectric immunosensor on the basis of immunoglobulin G Langmuir-Blodgett films for direct ferritin detection has been proposed. The detection range of ferritin concentrations in solution is 10(-10)-10(-7) M.
Lepesheva
G I
GI
Turko
I V
IV
Ges'
I A
IA
Chashchin
V L
VL
D000818:Animals; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D001665:Binding Sites; D004581:Electronics; D007118:Immunoassay; D007074:Immunoglobulin G / Q000276:immunology*; D011817:Rabbits
D000941:Antigens; D007074:Immunoglobulin G
Priamaia detektsiia antigena v plenkakh Léngmiura-Blodzhett immunoglobulinov G na osnove metoda poverkhnostnogo plazmonnogo rezonansa i v p'ezoélektricheskoĭ sisteme.