Crystallization and preliminary X-ray analysis of the Fab fragment of a human monoclonal IgM rheumatoid factor (2A2).
Crystals of the Fab fragment of a human monoclonal IgM rheumatoid factor have been obtained and are suitable for X-ray structure determination. This molecule, derived from the synovial B cells of a patient with rheumatoid arthritis, is an autoantibody with specificity for IgG Fc. The crystals have space group P2(1), cell dimensions a = 69.0 A, b = 76.6 A, c = 98.8 A and beta = 90.6 degrees, and diffract to a resolution of at least 2.8 A.
Sohi
M K
MK
Randall Institute, King's College London, U.K.
Sutton
B J
BJ
Corper
A L
AL
Wan
T
T
Maini
R N
RN
Brown
C
C
Rijnders
T
T
Beale
D
D
Feinstein
A
A
Humphreys
A S
AS
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007075:Immunoglobulin M / Q000737:chemistry*; D012217:Rheumatoid Factor / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D012217:Rheumatoid Factor