Functional interaction between the integrin antagonist neutrophil inhibitory factor and the I domain of CD11b/CD18.
Neutrophil inhibitory factor (NIF) is a hookworm-derived glycoprotein ligand of the integrin CD11b/CD18 that inhibits human neutrophil function (Moyle, M., Foster, D. L., McGrath, D. E., Brown, S. M., Laroche, Y., De Meutter, J., Stanssens, P., Bogowitz, C. A., Fried, V. A., Ely, J. A., Soule, H. R., and Vlasuk, G. P. (1994) J. Biol. Chem. 269, 1008-10015). Here, we present evidence that recombinant NIF (rNIF) associates with the approximately 200-amino acid residue I domain of CD11b/CD18 and that this interaction is essential for inhibition of neutrophil function by NIF. First, radiolabeled rNIF binds to a recombinant glutathione S-transferase fusion protein that contains the CD11b I domain. This high affinity interaction has a partial dependence on divalent cations. The association of rNIF with the CD11b I domain is specific because 125I-rNIF does not bind either a glutathione S-transferase fusion protein that contains the I domain of the integrin CD11a/CD18 or recombinant glutathione S-transferase without the I domain. Second, the CD11b I domain fusion protein effectively competes with CD11b/CD18 on human neutrophils for 125I-rNIF binding. Third, the CD11b I domain fusion protein blocks the inhibition of certain neutrophil functions by rNIF, including adhesion of neutrophils to human endothelial cell monolayers and adhesion-dependent release of hydrogen peroxide from neutrophils. Specificity is demonstrated by the inability of the CD11a I domain fusion protein to block either rNIF binding to neutrophils or rNIF activity. Fourth, rNIF blocks the interaction between neutrophils and fibrinogen, a CD11b/CD18 ligand that is also thought to bind the I domain of CD11b. In contrast, rNIF does not appear to block the binding of factor X to CD11b/CD18 on neutrophils. These results suggest that CD11b/CD18 has multiple distinct binding sites for its cognate ligands, including, but not limited to, the I domain. NIF interferes with the binding of a subset of these CD11b/CD18 ligands in a highly selective manner.
Muchowski
P J
PJ
Corvas International, Inc., San Diego, California 92121.
Zhang
L
L
Chang
E R
ER
Soule
H R
HR
Plow
E F
EF
Moyle
M
M
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D018845:CD11 Antigens / Q000378:metabolism; D016466:CHO Cells; D006224:Cricetinae; D006023:Glycoproteins / Q000378:metabolism* / Q000494:pharmacology; D015801:Helminth Proteins / Q000378:metabolism* / Q000494:pharmacology; D016023:Integrins / Q000037:antagonists & inhibitors*; D016177:Macrophage-1 Antigen / Q000378:metabolism*; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects*; D011994:Recombinant Proteins / Q000378:metabolism
D018845:CD11 Antigens; D006023:Glycoproteins; D015801:Helminth Proteins; D016023:Integrins; D016177:Macrophage-1 Antigen; D008565:Membrane Proteins; D011994:Recombinant Proteins; C086581:NIF protein, Ancylostoma caninum