Identification of metabolic pathways of the lipid peroxidation product 4-hydroxynonenal by mitochondria isolated from rat kidney cortex.
The cytosolic lipid peroxidation product 4-hydroxynonenal (HNE) is rapidly metabolized in mitochondria isolated from rat kidney cortex. About 80% of HNE was degraded within 3 min of incubation. Main products of HNE which were identified in mitochondria were the hydroxynonenoic acid, the 1,4-dihydroxynonene and the glutathione-HNE-conjugate. Furthermore, formation of metabolites of the tricarboxylic acid cycle from HNE is suggested. The quantitative share of HNE binding to proteins was high with about 8% of total HNE consumption after 3 min of incubation. Therefore, rapid degradation of HNE by mitochondria might be involved in an intracellular antioxidative defense system.
Ullrich
O
O
Clinic of Physical Therapy, Medical Faculty (Charite), Humboldt-University, Berlin, Germany.
Grune
T
T
Henke
W
W
Esterbauer
H
H
Siems
W G
WG
D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation; D007672:Kidney Cortex / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar
D000447:Aldehydes; C027576:4-hydroxy-2-nonenal