A truncated form of the IRF-2 transcription factor has the properties of a postinduction repressor of interferon-beta gene expression.
The interferon-beta promoter binding protein, IRF-2, is proteolytically processed during induction by double-stranded RNA to leave an N-terminal fragment that can still bind to DNA. An N-terminal fragment that corresponds to the induction-specific product has a higher affinity for the promoter and is a more potent repressor of interferon-beta transcription than the full-length precursor. The kinetics of production of the cleavage product is slower than that of activation of interferon-beta transcription. These results suggest that cleavage of IRF-2 functions to generate a postinduction repressor of interferon-beta expression.
Whiteside
S T
ST
Gene Expression Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
King
P
P
Goodbourn
S
S
D000818:Animals; D002478:Cells, Cultured; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D050856:Interferon Regulatory Factor-1; D050836:Interferon Regulatory Factor-2; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics; D008957:Models, Genetic; D010446:Peptide Fragments / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D004268:DNA-Binding Proteins; C494257:IRF1 protein, human; C494229:IRF2 protein, human; C090091:In4 complex; D050856:Interferon Regulatory Factor-1; D050836:Interferon Regulatory Factor-2; D010446:Peptide Fragments; D010750:Phosphoproteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D016899:Interferon-beta