Proof of fallout endothelialization of impervious Dacron grafts in the aorta and inferior vena cava of the dog.
OBJECTIVE
The purpose of this study was to prove directly whether cells from the blood stream contribute to endothelialization of isolated, impervious Dacron vascular grafts in the dog.


METHODS
We designed an 18 cm, three-component graft with two parallel central Dacron limbs; one was made impervious with silicone rubber, and the other was preclotted. This model was implanted in the canine descending thoracic aorta with 30 microns polytetrafluoroethylene grafts anastomosed at each end. An 8 cm, three-component graft completely coated with silicone rubber was implanted in the canine abdominal aorta and inferior vena cava. Implant periods ranged from 4 to 12 weeks. Flow surfaces were studied by use of stereomicroscopy after being stained with silver nitrate, and by use of scanning and transmission electron microscopy, the inner wall and flow surface were studied by light microscopy after hematoxylin-eosin and immunocytochemical staining (the latter for endothelial and smooth muscle cells), and the full wall was studied by light microscopy after hematoxylin-eosin staining.


RESULTS
Effective prevention of pannus and transmural ingrowth into the impervious central test grafts was achieved, and scattered islands of endothelial cells were conclusively demonstrated on flow surfaces in each of the three implant sites 4 weeks after implantation. In the descending thoracic aorta, where these grafts were also implanted for 8 and 12 weeks, alpha-actin-positive cells and microvessels were found beneath some of the endothelial islands.


CONCLUSIONS
Fallout endothelialization of Dacron vascular grafts occurs in both the arterial and venous systems of the dog.
Shi
Q
Q
Hope Heart Institute, University of Washington School of Medicine, Seattle 98122.
Wu
M H
MH
Hayashida
N
N
Wechezak
A R
AR
Clowes
A W
AW
Sauvage
L R
LR
D000818:Animals; D001012:Aorta, Abdominal / Q000166:cytology*; D001013:Aorta, Thoracic / Q000166:cytology*; D001807:Blood Vessel Prosthesis; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D017348:Microscopy, Electron, Scanning Transmission; D008954:Models, Biological; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011093:Polyethylene Terephthalates; D019736:Prostheses and Implants; D012826:Silicone Elastomers; D013499:Surface Properties; D013997:Time Factors; D014682:Vena Cava, Inferior / Q000166:cytology*
D011093:Polyethylene Terephthalates; D012826:Silicone Elastomers