Cloning of a somatolactin-encoding cDNA from sole (Solea senegalensis).
From a Solea senegalensis cDNA expression library, clones encoding somatolactin (SL), a new pituitary hormone belonging to the growth hormone/prolactin family, were isolated and analyzed. Northern blot analysis showed a unique 1.0-kb mRNA species. The sole SL 778-bp cDNA encoded full-size S. senegalensis SL (ssSL) (230 amino acids), including seven Cys and two potential glycosylation sites. A consensus polyadenylation signal, AATAAA, was found. Protein homology and DNA sequence alignment of SL cDNAs from other evolutionarily distant marine fishes suggest that the SL sequence is highly conserved.
Pendón
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Department of Biochemistry and Molecular Biology, Facultad de Ciencias, Universidad de Cádiz, Spain.
Martínez-Barberá
J P
JP
Valdivia
M M
MM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D029941:Fish Proteins; D005412:Flatfishes / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010907:Pituitary Hormones / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D029941:Fish Proteins; D006023:Glycoproteins; D010907:Pituitary Hormones; C064448:somatolactin protein, fish