title: Thiopurine methyltransferase activity in a French population: h.p.l.c. assay conditions and effects of drugs and inhibitors.
abstract: 1. Thiopurine methyltransferase (TPMT) is a cytosolic enzyme involved in the catabolism of thiopurine drugs, which are used to treat cancer patients and organ transplant recipients. Because TPMT activity is polymorphic and under genetic control, large interindividual variations in the immunosuppressive activity and toxicity of these drugs may, at least in part, be inherited. 2. We have developed a specific h.p.l.c. method for measuring 6-methyl mercaptopurine formed from 6-mercaptopurine (6-MP) in red blood cell lysates during the TPMT assay procedure. In blinded assays of 55 samples from adult blood donors, the results of the h.p.l.c. method correlated with those of the radiochemical reference method (r = 0.83, P < 0.001). 3. Using this h.p.l.c. assay, we tested the effect of known inhibitors of TPMT activity (syringic acid, p-anisic acid and tropolone) in vitro and showed that they were highly inhibitory. We also found that drugs often administered concomitantly with 6-MP (prednisone, prednisolone, 6-methylprednisolone, cyclophosphamide, methotrexate, and trimethoprim-sulphamethoxazole) had little or no effect on TPMT activity in vitro. 4. In a group of 300 French individuals, TMPT activity was highly variable, ranging from 4.7 to 35.3 nmol h-1 ml-1 of packed red blood cells (nmol h-1 ml-1 PRBC) with a mean value of 19.3 +/- 4.9. TMPT activity was not influenced by sex. 5. This sensitive and reproducible h.p.l.c. assay for TPMT activity in red blood cells may prove useful for prospective clinical studies designed to optimise dosage regimens of thiopurine drugs (detection limit for 6-methyl mercaptopurine is 5 ng ml-1, intra- and inter-assay variations are 6.8 and 8.2%, respectively).
authors.affiliation: Service de Pharmacologie Clinique, Hôpital Robert Debré, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Jacqz-Aigrain
authors.forename: E
authors.initials: E
authors.lastname: Bessa
authors.forename: Y
authors.initials: Y
authors.lastname: Medard
authors.forename: Y
authors.initials: Y
authors.lastname: Mircheva
authors.forename: E
authors.initials: E
authors.lastname: Vilmer
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005602:France; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D008297:Male; D015122:Mercaptopurine / Q000031:analogs & derivatives / Q000097:blood; D008780:Methyltransferases / Q000037:antagonists & inhibitors / Q000097:blood* / Q000235:genetics; D010641:Phenotype; D011110:Polymorphism, Genetic
chemical_list: D005707:Gallic Acid; C010240:6-methylthiopurine; C001945:syringic acid; D015122:Mercaptopurine; D008780:Methyltransferases; C022745:thiopurine methyltransferase