Localization of Locusta-DP in locust CNS and hemolymph satisfies initial hormonal criteria.
Locusta-diuretic peptide (Locusta-DP) is a potent stimulant of fluid secretion and cyclic AMP production by locust Malpighian tubules. In this study, a polyclonal antiserum raised to the C-terminus of Locusta-DP reveals a wide distribution of immunoreactive cell bodies and processes throughout the CNS, and endings in two important neurohemal release sites: the corpora cardiaca and the perivisceral organs. HPLC fractionation of CNS, neurohemal structures, and hemolymph reveals immunoreactive material that coelutes with synthetic Locusta-DP and stimulates cyclic AMP production by locust tubules. The identity of the immunoreactive and biologically active material is confirmed as authentic Locusta-DP by mass spectrometry.
Patel
M
M
Department of Biology, Birkbeck College, London, UK.
Chung
J S
JS
Kay
I
I
Mallet
A I
AI
Gibbon
C R
CR
Thompson
K S
KS
Bacon
J P
JP
Coast
G M
GM
D000818:Animals; D002490:Central Nervous System / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006110:Grasshoppers / Q000737:chemistry*; D006458:Hemolymph / Q000737:chemistry*; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D009474:Neurons / Q000737:chemistry; D009479:Neuropeptides / Q000032:analysis*
D007301:Insect Hormones; D009479:Neuropeptides; C072468:diuretic peptide, Locusta-DP