Matrix-assisted laser desorption/ionization mass spectrometry of restriction enzyme-digested plasmid DNA using an active Nafion substrate.
Matrix-assisted laser desorption/ionization using a 3-hydroxypicolinic acid matrix from an active Nafion substrate has been used for detection of restriction enzyme-digested double-stranded plasmid DNA using time-of-flight mass spectrometry. DNA strands of up to 267 base pairs were detected with minimal sample purification, although only as species corresponding to single-stranded DNA.
Bai
J
J
Department of Chemistry, University of Michigan, Ann Arbor 48109.
Liu
Y H
YH
Lubman
D M
DM
Siemieniak
D
D
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000737:chemistry; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D006801:Humans; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010848:Picolinic Acids; D010957:Plasmids / Q000737:chemistry*; D016133:Polymerase Chain Reaction
D005465:Fluorocarbon Polymers; D010848:Picolinic Acids; C040402:perfluorosulfonic acid; C049246:3-hydroxypicolinic acid; D004247:DNA; D004262:DNA Restriction Enzymes