Regulation of phytochrome A mRNA abundance in parsley seedlings and cell-suspension cultures.
A cDNA clone encoding the apoprotein of a parsley phytochrome was isolated and classified as parsley PHYA phytochrome, on the basis of a sequence homology comparison with all available phytochrome sequences. Red light pulses led to a phytochrome-dependent down-regulation of PHYA mRNA abundance in etiolated parsley seedlings to a level of 10-20% compared with the dark control. The PHYA mRNA abundance in a parsley cell suspension culture was also down-regulated by light pulses. Transient expression assays in parsley protoplasts showed light regulation of a chimeric pea PHYA promoter uidA-gene construct.
Poppe
C
C
Institut für Biologie II, Universität Freiburg, Germany.
Ehmann
B
B
Frohnmeyer
H
H
Furuya
M
M
Schäfer
E
E
D000595:Amino Acid Sequence; D001059:Apoproteins / Q000235:genetics; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics; D008027:Light; D019684:Magnoliopsida / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010834:Phytochrome / Q000737:chemistry / Q000235:genetics*; D051942:Phytochrome A; D011523:Protoplasts; D012333:RNA, Messenger / Q000096:biosynthesis*; D018749:RNA, Plant / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001059:Apoproteins; D018076:DNA, Complementary; D051942:Phytochrome A; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D010834:Phytochrome