Screening of chloramphenicol residues in pork muscle by planar chromatography.
A rapid planar chromatographic method is described for the qualitative determination of chloramphenicol residues in muscle. The drug is extracted with ethyl acetate and purified by solid-phase extraction. After elution, the collected phase is evaporated to dryness. The residue is dissolved in methanol, spotted on a silica gel plate, and chromatographed. After elution, the chloramphenicol is visualized after reduction with fluorescamine. This method detects chloramphenicol in meat at 10 micrograms/kg and has a sample throughput of about 25 samples per analyst per day.
Abjean
J P
JP
Centre National d'Etudes Vétérinaires et Alimentaires, Laboratoire des Médicaments Vétérinaires, Fougeres, France.
D000818:Animals; D002701:Chloramphenicol / Q000032:analysis*; D002845:Chromatography / Q000379:methods* / Q000706:statistics & numerical data; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination; D009132:Muscles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013552:Swine
D002701:Chloramphenicol