New model for bone resorption study in vitro: human osteoclast-like cells from giant cell tumors of bone.
Cells harvested from 12 human giant cell tumors of bone and kept in culture for several passages were characterized for bone-resorbing capability, total and tartrate-resistant acid phosphatase activity, response to the calciotropic hormone calcitonin, cell proliferation, multinucleation after passages, and presence of calcium sensing. Cells obtained from three tumors presented a complete panel of osteoclast characteristics and maintained their multinuclearity after several passages. Cells from four other tumors increased their cAMP levels after treatment with calcitonin, and the other five apparently consisted of cells of stromal origin. These human cell populations with osteoclast characteristics may provide valid in vitro models for the investigation of osteoclastic differentiation and activity.
Grano
M
M
Institute of Human Anatomy, University of Bari, Italy.
Colucci
S
S
De Bellis
M
M
Zigrino
P
P
Argentino
L
L
Zambonin
G
G
Serra
M
M
Scotlandi
K
K
Teti
A
A
Zambonin Zallone
A
A
D000135:Acid Phosphatase / Q000378:metabolism*; D000328:Adult; D001859:Bone Neoplasms / Q000473:pathology*; D001862:Bone Resorption; D002116:Calcitonin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D005870:Giant Cell Tumors / Q000473:pathology*; D006801:Humans; D008297:Male; D008858:Microscopy, Phase-Contrast; D008875:Middle Aged; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D002116:Calcitonin; D000242:Cyclic AMP; D000135:Acid Phosphatase; D002118:Calcium