Evidence implicating both slow- and fast-conducting fibers in the rewarding effect of medial forebrain bundle stimulation.
A behavioral version of the collision test was used to determine whether reward-relevant neurons directly link self-stimulation sites in the lateral hypothalamic (LH) and ventral tegmental (VTA) areas. Five male rats served as subjects. Trains of conditioning (C) and test (T) pulses were delivered to the two stimulation sites, each site receiving one of the pulses from each pair. The C-T interval was varied from 0.2-17.3 ms, and the effectiveness of the paired pulse stimulation was estimated by comparing the rate-number curve obtained at each C-T interval to rate-number curves obtained with trains of evenly spaced single pulses delivered via one electrode. For 4 of the subjects, stimulation effectiveness increased with the C-T interval, and the form of this increase was similar regardless of which electrode delivered the C-pulses. These increases in effectiveness are consistent with recovery from collision block in reward-relevant fibers stimulated at both sites. The domain of the rising portion of the effectiveness versus C-T interval curve spanned 2.2-7.7 ms. Such a gradual rise suggests that the directly stimulated substrate is composed of fibers with a wide range of conduction velocities and/or refractory periods. The discrepancy between these gradually rising collision curves and the steeply rising curves obtained in previous collision studies may have been due to inadequate sampling of the rate-number function in the earlier studies.
Murray
B
B
Department of Psychology, Concordia University, Montréal, Qué, Canada.
Shizgal
P
P
D000818:Animals; D001931:Brain Mapping; D004558:Electric Stimulation; D007026:Hypothalamic Area, Lateral / Q000502:physiology; D008297:Male; D008474:Medial Forebrain Bundle / Q000502:physiology*; D009042:Motivation; D009412:Nerve Fibers / Q000502:physiology*; D009434:Neural Pathways / Q000502:physiology; D051381:Rats; D011930:Reaction Time / Q000502:physiology*; D012032:Refractory Period, Electrophysiological / Q000502:physiology; D012653:Self Stimulation / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*; D013681:Tegmentum Mesencephali / Q000502:physiology