Jacalin, a lectin that inhibits in vitro HIV-1 infection, induces intracellular calcium increase via CD4 in cells lacking the CD3/TcR complex.
The lectin jacalin interacts with the CD4 cell surface antigen; this lectin inhibits in vitro infection by human immunodeficiency virus type 1 without preventing virus binding on the host cell. The infection process is known to involve cellular events triggered by the binding of the viral external glycoprotein gp120 to CD4. Herein we demonstrate that jacalin induces cell signaling directly through the CD4 antigen and that independently of the CD3/TcR complex. The capacity of jacalin to trigger cell signals through the CD4 molecule is discussed in relation to its ability to inhibit HIV infection.
Lafont
V
V
INSERM U65, Université de Montpellier II, France.
Dornand
J
J
d'Angeac
A D
AD
Monier
S
S
Alcover
A
A
Favero
J
J
D017252:CD3 Complex / Q000502:physiology; D015704:CD4 Antigens / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D019833:Genistein; D006801:Humans; D066298:In Vitro Techniques; D007529:Isoflavones / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology*; D037121:Plant Lectins; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000502:physiology; D016176:T-Lymphocyte Subsets / Q000378:metabolism
D017252:CD3 Complex; D015704:CD4 Antigens; D007529:Isoflavones; D037102:Lectins; D037121:Plant Lectins; D016693:Receptors, Antigen, T-Cell, alpha-beta; C043696:jacalin; D019833:Genistein; D002118:Calcium