title: DNA content in non-Hodgkin's lymphoma. Comparison between flow cytometry and cytogenetics in fresh and paraffin-embedded tissue.
abstract: DNA content of 36-non-Hodgkin's lymphomas was analyzed by flow cytometry (FCM) and cytogenetics (CG), 21 in fresh and 15 in paraffin-embedded tissue. The results of both techniques were coincident in 60% of the fresh tissue samples and in 45% of the paraffin-embedded ones, the reason for this difference could be the poor resolution of DNA histograms from paraffin-embedded tissue. All samples judged as aneuploid by FCM were aneuploid also by CG. Some samples with a hyperdiploid population by CG gave a diploid population by FCM with a 'false' high DNA-synthesis (S) fraction. From a technical point of view, CG and FCM have to be performed on the same fresh tissue.
authors.affiliation: Department of Immunology, Fundacion Jiménez Díaz, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: García
authors.forename: A
authors.initials: A
authors.lastname: Bueno
authors.forename: E
authors.initials: E
authors.lastname: Feijoó
authors.forename: M
authors.initials: M
authors.lastname: Robledo
authors.forename: G
authors.initials: G
authors.lastname: Echezarreta
authors.forename: F
authors.initials: F
authors.lastname: Ortíz
authors.forename: J
authors.initials: J
authors.lastname: Benítez
authors.forename: J L
authors.initials: JL
authors.lastname: Sarasa
authors.forename: C
authors.initials: C
authors.lastname: Rivas
mesh_terms: D002453:Cell Cycle; D003584:Cytological Techniques; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006689:Hodgkin Disease / Q000235:genetics / Q000473:pathology*; D006801:Humans; D016612:Paraffin Embedding; D011003:Ploidies
chemical_list: D004273:DNA, Neoplasm