title: A nuclear photosynthetic electron transport mutant of Arabidopsis thaliana with altered expression of the chloroplast petA gene.
abstract: The nuclear photosynthetic mutant, hcf2, of Arabidopsis thaliana was isolated by screening M2 seedlings for abnormally-high chlorophyll fluorescence (hcf), indicative of a block in photosynthetic electron transport. Fluorescence induction kinetics, photosynthetic electron transport activity assays and immunoblotting revealed that all the complexes involved in photosynthetic electron transport were affected to some extent. The most striking effect of the mutation was on the relative steady state levels of the petA transcript (encoding the apoprotein of cytochrome f) which were more than five-times higher in the mutant plants than in their wild-type siblings.
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, Canada.
authors.forename: R D
authors.initials: RD
authors.lastname: Dinkins
authors.forename: H
authors.initials: H
authors.lastname: Bandaranayake
authors.forename: B R
authors.initials: BR
authors.lastname: Green
authors.forename: A J
authors.initials: AJ
authors.lastname: Griffiths
mesh_terms: D001059:Apoproteins / Q000096:biosynthesis* / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics*; D002338:Carotenoids / Q000378:metabolism; D002467:Cell Nucleus; D002734:Chlorophyll / Q000737:chemistry; D002736:Chloroplasts / Q000737:chemistry; D003580:Cytochromes / Q000096:biosynthesis* / Q000235:genetics; D045348:Cytochromes f; D004579:Electron Transport / Q000235:genetics*; D005453:Fluorescence; D005786:Gene Expression Regulation; D017343:Genes, Plant; D007425:Intracellular Membranes / Q000737:chemistry; D009154:Mutation; D010788:Photosynthesis / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D019207:beta Carotene
chemical_list: D001059:Apoproteins; D003580:Cytochromes; D010940:Plant Proteins; D012333:RNA, Messenger; D019207:beta Carotene; D002734:Chlorophyll; D002338:Carotenoids; D045348:Cytochromes f