No evidence of mycoplasmas in peripheral blood mononuclear cell fraction of HIV-infected patients.
In this study, the prevalence of mycoplasmas in peripheral blood mononuclear cells (PBMC) from HIV-infected individuals was investigated using a mycoplasma genus-specific PCR assay. No mycoplasmas were detected in the PBMC samples from any of the 25 HIV-infected individuals (CDC 2, n = 8; CDC 3, n = 2; CDC 4, n = 15) or ten HIV-seronegative controls. As an internal control, HIV specific sequences were detected in the samples from all HIV-seropositives. These negative results do not support a suggested role of mycoplasmas as co-factor in the progression of HIV infection towards AIDS.
van Kuppeveld
F
F
Dept. of Microbiology, University of Nijmegen, The Netherlands.
Melchers
W
W
Kissing
J
J
van der Logt
J
J
Galama
J
J
D017088:AIDS-Related Opportunistic Infections / Q000097:blood / Q000150:complications / Q000453:epidemiology*; D001483:Base Sequence; D016022:Case-Control Studies; D004269:DNA, Bacterial; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D009175:Mycoplasma Infections / Q000097:blood* / Q000150:complications / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012307:Risk Factors
D004269:DNA, Bacterial