[Photodynamic effect of chlorophyll derivative (CPD4) with pulsed copper-vapor laser on mouse transplanted tumor].
An experimental study on photodynamic effect of newly developed photosensitizer CPD4 with pulsed copper-vapor laser and pumped dye laser to mouse transplanted tumor (CT26) was carried out. The results showed that: (1) Ten days after treatment by CPD4 with 630nm laser, the mean tumor volume of control group (n = 9) was 1.0 +/- 0.68cm3, while that of treatment group (n = 18) was 0.45 +/- 0.5cm3 (P < 0.01), with an inhibitory rate of 55%. In the treatment group, 8 mice that were treated with a dose > or = 50mg/kg(iv) of CPD4, 280mW/cm2 of laser power density and 10 minutes of irradiation, were all cured with survival time over 6 months, while that of mice in the control group was 28.2 days (2) Under certain condition (light energy 120 J/cm2), with CPD4 as a sensitizer, the mean depths of tumor necrosis were 4.9 +/- 1.0mm (n = 5), 2.8 +/- 1.1mm(n = 11), 1.8 +/- 0.5mm(n = 9) for wavelength of 670nm, 630nm and 510nm, respectively (P < 0.01). When a conventional photosensitizer hematoporphyrin derivative was used, the depths of tumor necrosis were 0 (n = 6) 2.3 +/- 0.3 (n = 5) and 2.8 +/- 1.2 (n = 5)mm for 670, 630 and 510nm, respectively. Based on the above mentioned results, a rational treatment condition in clinical application of CPD4 with copper-vapor laser and pumped dye laser was suggested.
Cui
J H
JH
Cancer Institute, Zhejiang Medical University, Hangzhou.
Zheng
S
S
Wu
J M
JM
D000818:Animals; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000627:therapeutic use; D003110:Colonic Neoplasms / Q000188:drug therapy*; D005260:Female; D017324:Hematoporphyrin Derivative / Q000627:therapeutic use; D007834:Lasers; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use*
D017319:Photosensitizing Agents; D002734:Chlorophyll; D017324:Hematoporphyrin Derivative