Effect of opioid peptide antisera on nitrous oxide antinociception in rats.
This study was performed to examine the effects of ICV injection of antiserum against beta-endorphin (beta-EP) or methionine-enkephalin (ME) on nitrous oxide-induced antinociception in rats using the hot plate test. The injection of beta-EP antiserum reversed the antinociceptive effect of nitrous oxide in a dose-related manner up to 200 micrograms/rat. However, antagonism of nitrous oxide by 400 micrograms beta-EP antiserum was comparable to that produced by 200 micrograms. On the other hand, similar amounts of ME antiserum had little effect against nitrous oxide antinociception. These findings suggest that beta-EP may play an important role in the antinociceptive effect of nitrous oxide.
Hara
S
S
Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford 61107.
Gagnon
M J
MJ
Quock
R M
RM
Shibuya
T
T
D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D004744:Enkephalin, Methionine / Q000276:immunology; D007276:Injections, Intraventricular; D008297:Male; D009609:Nitrous Oxide / Q000494:pharmacology*; D018847:Opioid Peptides / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013238:Stereotaxic Techniques; D001615:beta-Endorphin / Q000276:immunology
D000700:Analgesics; D000906:Antibodies; D018847:Opioid Peptides; D004744:Enkephalin, Methionine; D001615:beta-Endorphin; D009609:Nitrous Oxide