The modification of hemoglobin by a long crosslinking reagent: bis(3,5-dibromosalicyl) sebacate.
Bis(3,5-dibromosalicyl) sebacate is a bifunctional protein crosslinking reagent. It reacts with oxy form of human hemoglobin A to produce a crosslinked hemoglobin between two beta chains in the 2,3-bisphosphoglycerate binding cleft, decreasing the oxygen affinity. The oxygen binding curve of crosslinked hemoglobin had a P50 of 18.5 mmHg compared to a P50 of 11.0 mmHg for native hemoglobin and remains highly cooperative, n = 2.2. Crosslinking hemoglobin between the two beta chains also results in a 12.5 degrees C increase in the thermal denaturation temperature. The crosslinked hemoglobin is oxidized more rapidly to methemoglobin. Its autoxidation rate in 0.01 M MOPS, pH 7.4, at 37 degrees C was 1.4 times as fast as that of Hb A.
Zhang
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Department of Chemistry Loyola University of Chicago, IL 60626.
Olsen
K W
KW
D019794:2,3-Diphosphoglycerate; D001665:Binding Sites; D003432:Cross-Linking Reagents; D003652:Decanoic Acids / Q000737:chemistry* / Q000378:metabolism; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D006441:Hemoglobin A / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D008706:Methemoglobin / Q000737:chemistry; D010108:Oxyhemoglobins / Q000737:chemistry* / Q000378:metabolism; D011489:Protein Denaturation; D012459:Salicylates / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
D003432:Cross-Linking Reagents; D003652:Decanoic Acids; D004163:Diphosphoglyceric Acids; D046911:Macromolecular Substances; D010108:Oxyhemoglobins; D012459:Salicylates; D019794:2,3-Diphosphoglycerate; C089663:bis(3,5-dibromosalicyl)sebacate; D008706:Methemoglobin; D006441:Hemoglobin A; C029774:oxyhemoglobin A