Induction of the conversion of xanthine dehydrogenase to oxidase in rabbit liver by Cu2+,Zn2+ and selenium ions.
Effects of Cu2+,Zn2+,Fe2+ and selenium ions on the conversion of xanthine dehydrogenase to oxidase in rabbit liver were examined. Under basal conditions, xanthine oxidase activity represented only 16% of the total xanthine oxidase plus dehydrogenase activity. Cu2+ (2-10 microM), Zn2+ (5-30 microM) and selenium ions (5-100 microM) brought about the conversion of xanthine dehydrogenase to oxidase in a dose-dependent manner. The concentrations of Cu2+,Zn2+ and selenium ions required for increasing xanthine oxidase activity by 50% was approximately 4, 10 and 20 microM, respectively. On the other hand, Fe2+ had no effect on the conversion of the enzyme up to 100 microM. These results suggest that Cu2+,Zn2+ and selenium ions have the potential to modulate the conversion of xanthine dehydrogenase to oxidase in rabbit liver.
Sakuma
S
S
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
Fujimoto
Y
Y
Iwai
M
M
Fujimoto
K
K
Yasuda
M
M
Nishida
H
H
Fujita
T
T
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000494:pharmacology*; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D011817:Rabbits; D012643:Selenium / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology; D014527:Uric Acid / Q000032:analysis; D014968:Xanthine Dehydrogenase / Q000187:drug effects / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000187:drug effects / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology*
D014527:Uric Acid; D000493:Allopurinol; D003300:Copper; D007501:Iron; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase; D014357:Trypsin; D012643:Selenium; D015032:Zinc