title: Structural organization of str 246C and str 246N, plant defense-related genes from Nicotiana tabacum.
abstract: We have previously identified a cDNA clone, pNt246, whose corresponding transcripts accumulate in leaves in response to inoculation by compatible and incompatible isolates of the phytopathogenic bacterium Pseudomonas solanacearum [19]. We now describe the nucleotide sequence of a genomic clone, str 246C, corresponding to this cDNA species, and of a related genomic clone, str 246N, which appears to be a pseudogene with a 5'-end deletion. The nucleotide sequence of the str 246C gene was found to be identical to that of the parA gene, previously shown to be regulated by auxin [28, 29]. Upstream of the str 246N gene, sequences homologous to a Bam HI repetitive element described in Vicia faba [15] are present within an ORF showing significant homologies to an integrase-encoding gene of several retroviruses. This observation indicates that this highly repetitive DNA originates from sequences present in transposable mobile elements.
authors.affiliation: Laboratoire de Botanique, Toulouse, France.
authors.forename: D
authors.initials: D
authors.lastname: Froissard
authors.forename: C
authors.initials: C
authors.lastname: Gough
authors.forename: P
authors.initials: P
authors.lastname: Czernic
authors.forename: M
authors.initials: M
authors.lastname: Schneider
authors.forename: A
authors.initials: A
authors.lastname: Toppan
authors.forename: D
authors.initials: D
authors.lastname: Roby
authors.forename: Y
authors.initials: Y
authors.lastname: Marco
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis; D017343:Genes, Plant / Q000235:genetics*; D019426:Integrases; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010947:Plants, Toxic; D011544:Pseudogenes / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; C090432:STR246N protein, Nicotiana tabacum; D004254:DNA Nucleotidyltransferases; D019426:Integrases