Effects of 8-chloroadenosine-3',5'-monophosphate in combination with irradiation in L5178Y mouse lymphoblasts.
The effect of a new anticancer drug, 8-chloroadenosine 3',5'-monophosphate (8-Cl-cAMP-), a site selective cAMP analog, that inhibits growth of cancer cells in vitro, was examined in L5178Y (LY) murine lymphoma cells. Two LY sublines were used, grown in full Fisher's medium: LY-R, radiation resistant and LY-S, radiation sensitive. The latter was also adapted to grow in simplified medium. In the full medium conversion of 8-Cl-cAMP to 8-chloroadenosine presumably was the case of cytotoxicity. In the simplified medium this conversion was limited and the cytotoxic effect much less pronounced. Cytotoxicity was equal in LY-R and LY-S cells and it was not related to changes in the cell cycle distribution; the latter were observed in LY-S, but not in LY-R cells. There was no interaction of the drug with x-rays in LY cells grown either in full or simplified medium.
Buraczewska
I
I
Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland.
Szumiel
I
I
Zagórski
S
S
Afanasjev
G G
GG
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000627:therapeutic use; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003131:Combined Modality Therapy; D004354:Drug Screening Assays, Antitumor; D007155:Immunologic Factors / Q000627:therapeutic use*; D008223:Lymphoma / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy*; D051379:Mice; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D007155:Immunologic Factors; D015124:8-Bromo Cyclic Adenosine Monophosphate; C044258:8-chloro-cyclic adenosine monophosphate