title: Genomic fingerprinting of Yersinia enterocolitica species by degenerate oligonucleotide-primed polymerase chain reaction.
abstract: A novel version of the polymerase chain reaction (PCR) termed degenerate oligonucleotide-primed PCR (DOP-PCR) was used to fingerprint the bacterial genome of Yersinia enterocolitica species. Eight well-characterized reference strains of Y. enterocolitica (ribotype, serotype, biotype and zymotype) were examined. Optimal experimental conditions for reproducibility, sensitivity and specificity were obtained using a single DOP-primer. All the strains gave distinct DOP-PCR profiles composed of 12 to 19 fragments with sizes from 200 to 1.500 bp. Thus, the DOP-PCR, which has so far been used to fingerprint human, mouse, fruitfly and plant DNA, is also well-suited to bacterial DNA.
authors.affiliation: Laboratoire de Biochimie Génétique, Hôpital Robert Debré, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Sayada
authors.forename: B
authors.initials: B
authors.lastname: Picard
authors.forename: J
authors.initials: J
authors.lastname: Elion
authors.forename: R
authors.initials: R
authors.lastname: Krishnamoorthy
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015008:Yersinia enterocolitica / Q000235:genetics*
chemical_list: D017931:DNA Primers