title: The detection of the sialoglycoprotease gene and assay for sialoglycoprotease activity among isolates of Pasteurella haemolytica A1 strains, serotypes A13, A14, T15 and A16.
abstract: Polymerase chain reaction (PCR) using specific primers to the sialoglycoprotease gene (gcp) of Pasteurella haemolytica biotype A, serotype 1 amplified a 1-kb fragment from each of P. haemolytica serotypes A7, A13, A14 and A16, but not T15; which was confirmed by Southern blot hybridization analysis. Using a sialoglycoprotease (Gcp) activity assay, Gcp activity was found in serotypes A13, A14 and A16. Inclusion of these three serotypes confirms that all recognized A biotypes are positive for both gcp gene and activity, with the exception of serotype A11 (which has a different genetic organization and exhibits no Gcp activity). Furthermore, all recognized T biotypes are negative for both the gene and Gcp activity.
authors.affiliation: Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
authors.forename: C W
authors.initials: CW
authors.lastname: Lee
authors.forename: R Y
authors.initials: RY
authors.lastname: Lo
authors.forename: P E
authors.initials: PE
authors.lastname: Shewen
authors.forename: A
authors.initials: A
authors.lastname: Mellors
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002417:Cattle; D015342:DNA Probes; D015964:Gene Expression Regulation, Bacterial; D005819:Genetic Markers; D016978:Mannheimia haemolytica / Q000201:enzymology* / Q000235:genetics; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D015342:DNA Probes; D005819:Genetic Markers; D008666:Metalloendopeptidases; C070738:O-sialoglycoprotein endopeptidase