Physiological expression of the 2',5'-oligoadenylate synthetase gene in mouse intestine.
2',5'-Oligoadenylate synthetase (2-5A synthetase) is an enzyme induced by interferon (IFN) that is considered to play an important role in IFN action. The normal mouse, not treated exogenously with any IFN or IFN inducers, has been shown to have an enhanced level of 2-5A synthetase activity, which is distributed among several lymphoid tissues. In the present report, we investigated the expression of the 42-kD 2-5A synthetase mRNA in the organs of normal mice using RNA blot hybridization and reverse transcriptase polymerase chain reaction (RT-PCR). Among the organs tested, intestinal tissues had the highest levels of this mRNA. Furthermore, the 42-kD 2-5A synthetase mRNA was expressed in intestines from germ-free mice and fetuses. Immunoblotting analysis using a monoclonal antibody against the 42-kD 2-5A synthetase revealed that the 42-kD enzyme as well as a cross-reactive protein of 30 kD were produced in the organs of the normal mouse.
Sokawa
Y
Y
Department of Biotechnology, Kyoto Institute of Technology.
Kitano
Y
Y
Shimada
M
M
Okumura
M
M
Sokawa
J
J
Ueda
K
K
Hamada
K
K
Takeda
K
K
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D005314:Embryonic and Fetal Development / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005856:Germ-Free Life; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000196:embryology / Q000254:growth & development; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity / Q000502:physiology; D016133:Polymerase Chain Reaction; D012016:Reference Values
D015088:2',5'-Oligoadenylate Synthetase