title: Induction of autoimmunity by immunization of mice with human thyrotropin receptor.
abstract: The development of autoimmunity was investigated after repeated immunizations with human thyrotropin receptor (hTSH-R) of five congenic strains of female and male mice. After each immunization, free T3 levels and antibodies to hTSH-R and to six peptides of the hTSH-R were assayed. Our results showed that H-2s and H-2q female mice developed features of autoimmunity such as antibody responses to hTSH-R and to hTSH-R peptides, transient variations in the levels of free T3 thyroid hormone, and lymphocytic infiltrations in their thyroid glands. Concerning the antibody responses to hTSH-R peptides, we found that peptide P1 (352-366) contained a major B cell epitope. Furthermore, strain-specific B cell epitope was exemplified by peptide 92 (12-30) and two male- and female-specific B cell epitopes were located in peptides 91 (32-46) and 93 (316-330), respectively. These features appeared rather related to hyperthyroidism.
authors.affiliation: INSERM U. 283, Hôpital Cohin, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Marion
authors.forename: J M
authors.initials: JM
authors.lastname: Braun
authors.forename: A
authors.initials: A
authors.lastname: Ropars
authors.forename: L D
authors.initials: LD
authors.lastname: Kohn
authors.forename: J
authors.initials: J
authors.lastname: Charreire
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis*; D001667:Binding, Competitive / Q000276:immunology; D005260:Female; D006801:Humans; D008285:Major Histocompatibility Complex / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D011989:Receptors, Thyrotropin / Q000276:immunology* / Q000302:isolation & purification; D013954:Thyroglobulin / Q000276:immunology; D013961:Thyroid Gland / Q000473:pathology; D014284:Triiodothyronine / Q000097:blood
chemical_list: D001323:Autoantibodies; D010446:Peptide Fragments; D011989:Receptors, Thyrotropin; D014284:Triiodothyronine; D013954:Thyroglobulin