Stress response of Porphyromonas gingivalis.
The heat shock response of Porphyromonas gingivalis was examined by 1- and 2-dimensional polyacrylamide gel electrophoresis after metabolic labeling with [14C]-amino acids. When P. gingivalis cells were shifted from 37 degrees C to 42 degrees C, elevated synthesis of 4 proteins with the apparent molecular weight of 92, 80, 74, and 62 kDa was observed, and synthesis of a 50-kDa protein decreased during heat shock. The 74- and 62-kDa proteins were identified as homologs of Escherichia coli DnaK and GroEL respectively by Western immunoblotting. On 2-dimensional Western blots, 2 forms of DnaK and 4 forms of GroEL were identified due to their slightly different isolelectric points. dnaK and groEL gene homologs were identified in several P. gingivalis strains and some other black-pigmented oral species. DnaK and GroEL homolog proteins were induced when P. gingivalis cells were challenged by ethanol. These proteins were not induced by oxidative stress or change in pH.
Lu
B
B
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
McBride
B C
BC
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001439:Bacteroides / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D018834:Chaperonin 60 / Q000096:biosynthesis; D017124:Conserved Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000494:pharmacology; D006168:Guinea Pigs; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D018384:Oxidative Stress; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D018834:Chaperonin 60; D015342:DNA Probes; D004269:DNA, Bacterial; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D000431:Ethanol