[Epithelial and stromal cell culture of human endometrium and characterization].
Endometrium is a heterogeneous tissue, subject to many factors of which the actions and the regulations on the different cellular types were unknown. A medium with cortisol, cholera toxin, EGF, insulin and low concentration of horse serum is used for epithelial cell primary cultures (range 23-55 days, mean: 31.9 days). A medium with high concentration of fetal calf serum is used for stromal cell primary cultures (range 21-214 days, mean: 75 days). We have characterized by immunocytochemical studies all different cultures.
Merviel
P
P
Laboratoire de pharmacologie expérimentale et clinique, Hôpital St Louis, Paris.
Salat-Baroux
J
J
Calvo
F
F
D000328:Adult; D002478:Cells, Cultured; D002772:Cholera Toxin; D003470:Culture Media; D004717:Endometrium / Q000166:cytology*; D016228:Endothelial Growth Factors; D004848:Epithelium / Q000254:growth & development; D005069:Evaluation Studies as Topic; D005260:Female; D006064:Gonadotropins, Equine; D006801:Humans; D006854:Hydrocortisone; D007150:Immunohistochemistry; D007328:Insulin; D010641:Phenotype; D017154:Stromal Cells / Q000166:cytology; D013997:Time Factors
D003470:Culture Media; D016228:Endothelial Growth Factors; D006064:Gonadotropins, Equine; D007328:Insulin; D002772:Cholera Toxin; D006854:Hydrocortisone
Culture de cellules épithéliales et stromales de l'endometre humain et caractérisation.