title: Quantitative analysis of superoxide anion generation in living cells by using chemiluminescence video microscopy.
abstract: Superoxide anions (O2-) generated by rabbit neutrophils were detected and quantified by a video microscope equipped with a photon-counting camera. One count obtained by this system was equivalent to 59 amol of O2-. Maximum O2- production was observed at 6-8 min after stimulation and was estimated as 1.9 fmol/min per cell on the average.
authors.affiliation: Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Suzaki
authors.forename: E
authors.initials: E
authors.lastname: Kawai
authors.forename: Y
authors.initials: Y
authors.lastname: Kodama
authors.forename: T
authors.initials: T
authors.lastname: Suzaki
authors.forename: T
authors.initials: T
authors.lastname: Masujima
mesh_terms: D000818:Animals; D008163:Luminescent Measurements; D018715:Microscopy, Video / Q000379:methods*; D009504:Neutrophils / Q000737:chemistry*; D011817:Rabbits; D013481:Superoxides / Q000032:analysis*
chemical_list: D013481:Superoxides