Interleukin-1 receptor antagonist: an index of dialysis-induced interleukin-1 production.
Interleukin-1 receptor antagonist (IL-1Ra) is a specific inhibitor of interleukin-1 (IL-1). It is structurally similar to IL-1 and binds to IL-1 cell surface receptors, but does not transduce a signal. To examine the relationship between the production of IL-1Ra and IL-1, we incubated peripheral blood mononuclear cells (PBMC) with endotoxin (10 ng/ml) and after 24 h measured the total synthesis (intra- and extracellular) of IL-1Ra, IL-1 alpha and IL-1 beta. PBMC from 25 patients with chronic renal failure were studied. The total synthesis of IL-1Ra closely correlated with that of IL-1 beta (R = 0.786, p < 0.001). In contrast, the correlation between the total synthesis of IL-1Ra and IL-1 alpha was poor (R = 0.282, p > 0.05). These results suggest that in dialysis patients, the endogenous production of IL-1Ra is a 'footprint' of IL-1 beta production and can probably be used as a marker of the level of production of IL-1.
Pereira
B J
BJ
Division of Geographic Medicine and Infectious Disease, New England Medical Center Hospitals, Boston, Mass 02111.
King
A J
AJ
Falagas
M E
ME
Dinarello
C A
CA
D015415:Biomarkers / Q000097:blood; D004731:Endotoxins / Q000494:pharmacology; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000096:biosynthesis*; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D006435:Renal Dialysis; D012795:Sialoglycoproteins / Q000096:biosynthesis*
D015415:Biomarkers; D004731:Endotoxins; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins