An in vitro bioassay for nerve growth factor based on 24-hour survival of PC-12 cells.
We have developed an in vitro bioassay for nerve growth factor (NGF) which offers several advantages over currently used alternatives. The assay uses the commercially available PC-12 cell line, and requires only 24 hr incubation with NGF. The cells do not require priming, and are dosed as they are originally seeded in serum-free medium in 96-well cell culture plates coated with collagen. Concentrations between one and 20 ng/ml NGF can be measured, and only standard cell culture equipment and a microplate reader are required.
Robinson
C J
CJ
National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK.
Stammers
R
R
D000818:Animals; D001681:Biological Assay / Q000295:instrumentation; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen; D016895:Culture Media, Serum-Free; D009414:Nerve Growth Factors / Q000032:analysis* / Q000494:pharmacology; D016716:PC12 Cells / Q000187:drug effects*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
D016895:Culture Media, Serum-Free; D009414:Nerve Growth Factors; D011994:Recombinant Proteins; D003094:Collagen