Hepatic clearance of chylomicron remnants in mice lacking apoprotein E.
Control and apoprotein E-deficient mice generated by gene targeting in embryonic stem cells were fed a fat load containing 3H-labeled retinol and the absorbed radioactivity present in the plasma, liver, and carcass measured 6 h later. The radioactivity in the plasma of the apoprotein E-deficient mice was several fold higher than in control animals, but it accounted for less than 1/5 of the absorbed radioactivity. In both groups of animals most of the absorbed radioactivity was recovered in the livers. These findings indicate that in apoprotein E-deficient mice chylomicron remnants can be taken up by the liver by a process that does not require the mediation of apoprotein E.
Chang
S
S
Department of Pathology, Northwestern University Medical School, Chicago, IL 60611.
Zhang
S H
SH
Maeda
N
N
Borensztajn
J
J
D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency*; D002914:Chylomicrons / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D014316:Tritium; D014801:Vitamin A / Q000097:blood / Q000493:pharmacokinetics*
D001057:Apolipoproteins E; D002914:Chylomicrons; D014316:Tritium; D014801:Vitamin A