Therapeutic effect of recombinant vaccinia virus expressing the 60-kd heat-shock protein on adjuvant arthritis.
OBJECTIVE
To analyze the effects of recombinant viruses for 2 heat-shock proteins in the treatment of adjuvant arthritis.


METHODS
Virus vaccinia recombinant for mycobacterial heat-shock protein 65 (hsp65-VV) and human hsp60 (hsp60-VV) were administered to rats during different stages of adjuvant arthritis. Arthritis score and immunity to the recombinant virus were analyzed.


RESULTS
When delivered at the pre-arthritis stage, both constructs ameliorated arthritis; greater protection was observed with hsp60-VV. A specific T cell response to the recombinant proteins was detected. Furthermore, hsp60-VV displayed a clear therapeutic effect on established arthritis.


CONCLUSIONS
Our results suggest novel avenues of therapeutic intervention in autoimmune arthritis associated with immunity to hsp60.
López-Guerrero
J A
JA
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.
Ortiz
M A
MA
Páez
E
E
Bernabéu
C
C
López-Bote
J P
JP
D000818:Animals; D001169:Arthritis, Experimental / Q000150:complications / Q000517:prevention & control*; D001426:Bacterial Proteins; D018834:Chaperonin 60; D018833:Chaperonins; D006360:Heat-Shock Proteins; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000737:chemistry; D014616:Vaccinia virus / Q000737:chemistry / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000737:chemistry
D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins