title: Defective natural killer (NK) cell-mediated cytotoxicity does not imply clonal involvement of NK cells in myelodysplastic syndromes.
abstract: The clonality of purified cells was examined in 10 myelodysplastic syndromes (MDS) patients by analysing the restriction fragment length polymorphism and methylation pattern of the phosphoglycerate-kinase gene. Natural killer (NK) cell-mediated cytotoxicity was also examined. The granulocytes and monocytes were monoclonal or oligoclonal in all cases, except for the monocytes in one case. Conversely, the NK and T cells had a polyclonal pattern in most cases, including all cases who had defective NK cell-mediated cytotoxicity. The hypothesis that reduced NK cell-mediated cytotoxicity in MDS is caused by a clonal involvement of NK cells was not supported by the present study.
authors.affiliation: Third Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ogata
authors.forename: H
authors.initials: H
authors.lastname: Fujii
authors.forename: N
authors.initials: N
authors.lastname: Yokose
authors.forename: E
authors.initials: E
authors.lastname: An
authors.forename: H
authors.initials: H
authors.lastname: Tamura
authors.forename: K
authors.initials: K
authors.lastname: Kamikubo
authors.forename: K
authors.initials: K
authors.lastname: Dan
authors.forename: H
authors.initials: H
authors.lastname: Hamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Sakamaki
authors.forename: Y
authors.initials: Y
authors.lastname: Onozawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005260:Female; D006098:Granulocytes / Q000473:pathology; D006801:Humans; D007694:Killer Cells, Natural / Q000473:pathology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009000:Monocytes / Q000473:pathology; D009190:Myelodysplastic Syndromes / Q000276:immunology*; D010735:Phosphoglycerate Kinase / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D010735:Phosphoglycerate Kinase