Molecular analysis of two Ypt/Rab-related sequences isolated from soybean (Glycine max) DNA libraries.
From nodule and seedling cDNA libraries we isolated cDNA copies of two mRNAs, derived from the genes gmr1 and gmr2, encoding members of the Ypt/Rab family of small GTP-binding proteins. Two deduced protein products, GMR1 and GMR2, were found to be nearly identical differing by only four amino acids in the analysed parts. The two putative proteins are 79% identical to the previously described ARA small GTPase from Arabidopsis thaliana. The GMR proteins may thus be the counterpart of the ARA protein and may perform a related biological function in Glycine max. The gmr2 genomic sequence was isolated and structurally analysed. Expression analyses by northern and cDNA-based PCR showed that the gmr1 and gmr2 genes are constitutively expressed in different plant organs, although at a slightly higher level in callus culture. The classification of the gmr sequences as relatives of the Ypt/Rab family suggests that the deduced GMR proteins are involved in control of processes related to vesicle trafficking in plant cells.
Borg
S
S
Department of Molecular Biology, University of Aarhus, Denmark.
Poulsen
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D018744:DNA, Plant / Q000032:analysis; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D030262:Soybean Proteins; D013025:Glycine max / Q000235:genetics*; D020691:rab GTP-Binding Proteins
D018076:DNA, Complementary; D018744:DNA, Plant; C090421:GMR1 protein, soybean; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D030262:Soybean Proteins; D019204:GTP-Binding Proteins; D020691:rab GTP-Binding Proteins