title: Purification, expansion, and multiple fluorochrome labeling of cord blood hemopoietic precursors: preliminary results.
abstract: CD34-positive cells were isolated from a total of 23 cords using CellPro Ceprate columns. AIS MicroCellector flasks, and panning. The cells were (1) expanded in serum-free culture supplemented with a variety of combinations of cytokines and (2) immunophenotyped using multiple fluorochrome labeling. The results indicated that the avidin column produced the highest purity of CD34-positive cells, and that immature blast cells could be expanded in serum-free culture. Preliminary results suggested that the four fluorochrome labeling technique may provide useful information on the lineage commitment of cord blood precursor and blast cells.
authors.affiliation: Service of Biological, CHU du Sart-Tilman, Liège, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Feron
authors.forename: A
authors.initials: A
authors.lastname: Gothot
authors.forename: J C
authors.initials: JC
authors.lastname: Grosdent
authors.forename: J R
authors.initials: JR
authors.lastname: van Cauwenberge
authors.forename: J P
authors.initials: JP
authors.lastname: Schaaps
authors.forename: Y
authors.initials: Y
authors.lastname: Beguin
authors.forename: J M
authors.initials: JM
authors.lastname: Paulus
mesh_terms: D001360:Avidin; D001710:Biotin; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation / Q000295:instrumentation; D016895:Culture Media, Serum-Free; D016207:Cytokines / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology*; D005456:Fluorescent Dyes; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007163:Immunosorbent Techniques; D007231:Infant, Newborn
chemical_list: D016895:Culture Media, Serum-Free; D016207:Cytokines; D005456:Fluorescent Dyes; D001360:Avidin; D001710:Biotin