Effect of a traditional Chinese herbal medicine, kanzo-bushi-to, on the production of interleukin-4 from a clone of burn-associated CD8+ suppressor T cells.
Effect of Kanzo-bushi-to (TJS-038), one of the Japanese Kampo medicines originating from traditional Chinese herbal medicine, on the activity of a clone of burn-associated CD8+ suppressor T cells (T6S cells) was investigated. The suppressor cell activity of T6S cells in the mixed lymphocyte-tumor cell reaction (MLTR) was not changed when various concentrations of TJS-038 were added to the MLTR. The suppressor cell activity of T6S cells was greatly inhibited by CD3+ CD4+ CD28+ TCR alpha/beta+ Vicia villosa lectin-adherent cells (TJS cells) purified from spleen cells of mice treated with TJS-038 at an oral dose of 50 mg/kg. The suppressor cell activity of T6S cells was not demonstrated in the MLTR assay when anti-IL-4 monoclonal antibody was added. Also the production of IL-4 from T6S cells was not detected, when they were co-cultured with TJS cells. These results suggest that TJS-038 stimulates the generation of contrasuppressor T cells (TJS cells) that inhibit the suppressor cell activity of T6S cells and the production of IL-4 from T6S cells.
Kobayashi
M
M
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555.
Utsunomiya
T
T
Herndon
D N
DN
Pollard
R B
RB
Suzuki
F
F
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D002056:Burns / Q000276:immunology* / Q000473:pathology; D002478:Cells, Cultured; D002999:Clone Cells; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D015703:Antigens, CD; D004365:Drugs, Chinese Herbal; D016693:Receptors, Antigen, T-Cell, alpha-beta; D015847:Interleukin-4