Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA.
Three genetic complementation groups of rodent cells are defective for both repair of x-ray-induced double-strand breaks and V(D)J recombination. Cells from one group lack a DNA end-binding activity that is biochemically and antigenically similar to the Ku autoantigen. Transfection of complementary DNA (cDNA) that encoded the 86-kilodalton subunit of Ku rescued these mutant cells for DNA end-binding activity, x-ray resistance, and V(D)J recombination activity. These results establish a role for Ku in DNA repair and recombination. Furthermore, as a component of a DNA-dependent protein kinase, Ku may initiate a signaling pathway induced by DNA damage.
Smider
V
V
Department of Medicine, Stanford University Medical Center, CA 94305.
Rathmell
W K
WK
Lieber
M R
MR
Chu
G
G
D000818:Animals; D034961:Antigens, Nuclear; D002460:Cell Line; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000528:radiation effects*; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D004265:DNA Helicases; D004260:DNA Repair; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D015321:Gene Rearrangement; D005816:Genetic Complementation Test; D006801:Humans; D000072200:Ku Autoantigen; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D011836:Radiation Tolerance; D011995:Recombination, Genetic; D014162:Transfection
D034961:Antigens, Nuclear; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D004247:DNA; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen