Expression of MUC2-mucin in colorectal adenomas and carcinomas of different histological types.
The expression of mucin MUC2 was investigated in normal colonic tissue, in colonic adenomas and in carcinomas of the mucinous and non-mucinous type. The latter were subdivided into carcinomas originating from the adenoma-carcinoma sequence (ACS) and de novo (DN) carcinomas. The expression was assayed by immunohistochemistry with the monoclonal anti-MUC2 antibody CCP58 and by mRNA semiquantitation. MUC2 protein epitope CCP58 was strongly expressed in 21% of normal colonic tissues, in 40% of villous and in 48% of tubular adenomas. Mucinous carcinomas exhibited strong expression in 72%, ACS carcinomas in 21% and DN adenocarcinomas in none of the tumors investigated. Compared with the adjacent non-malignant tissue (transitional mucosa), CCP58 epitope expression in the tumor was higher in 74% of mucinous carcinomas, but equal or lower in 69% of ACS carcinomas and in 100% of de novo carcinomas. The alterations of MUC2 expression detected by immunohistochemistry in adenocarcinomas were confirmed on mRNA level. These data indicate that the MUC2 expression pattern is different in the 3 carcinoma types investigated. MUC2 over-expression occurs in the adenomatous tissue. It is always maintained in mucinous carcinomas, but frequently decreased in non-mucinous ACS carcinomas. DN carcinomas are most frequently associated with decreased expression of MUC2.
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M
Klinikum Steglitz, Freien Universität Berlin, Department of Gastroenterology, Germany.
Klussmann
E
E
Krüger-Krasagakes
S
S
Schmitt-Gräff
A
A
Stolte
M
M
Bornhoeft
G
G
Stein
H
H
Xing
P X
PX
McKenzie
I F
IF
Verstijnen
C P
CP
D002288:Adenocarcinoma, Mucinous / Q000378:metabolism* / Q000473:pathology; D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D018253:Adenoma, Villous / Q000378:metabolism / Q000473:pathology; D018256:Adenomatous Polyps / Q000378:metabolism / Q000473:pathology; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D017931:DNA Primers; D003937:Diagnosis, Differential; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D055262:Mucin-2; D009077:Mucins / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis
D014408:Biomarkers, Tumor; D017931:DNA Primers; C524625:MUC2 protein, human; D055262:Mucin-2; D009077:Mucins; D009363:Neoplasm Proteins; D012333:RNA, Messenger