Transformed cells producing the glycoprotein D of HSV-1 are resistant to infection with clinical strains of HSV.
The generality of the resistance exhibited by gD producing cells to HSV-1 infection was tested. We tested three different cell lines producing various amounts of gD for resistance against three HSV-1 strains. The strains used were the prototype laboratory F strain and two recently isolated low passage local clinical strains, VG and VD. The results indicate that: (i) the resistance of the cell lines is directly related to the amount of gD they produce, (ii) the cell lines showed greater resistance against the two local clinical HSV-1 strains than against the laboratory strain, and (iii) the resistance is not mediated at the level of virus adsorption to the cell membranes.
Sivropoulou
A
A
Department of Biology, Aristotelian University, Thessaloniki, Greece.
Arsenakis
M
M
D000818:Animals; D002461:Cell Line, Transformed / Q000821:virology; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000276:immunology* / Q000502:physiology; D007113:Immunity, Innate; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology*
D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1