title: [Analysis of the dopamine D2 receptor gene].
abstract: The methods, such as Southern blot hybridization, PCR-direct sequencing and PCR-SSCP, to detect sequence variants in the human dopamine D2 receptor (DRD2) gene were described. To study the association of a missense variant of the DRD2 gene resulting in Ser311-->Cys, which was detected by PCR-direct sequencing in schizophrenics, the genotypes of controls and schizophrenics were determined by the methods of PCR-dot blot hybridization, PCR amplification of specific alleles and artificially introduced PCR-RFLP.
authors.affiliation: Department of Medical Genetics, University of Tsukuba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Arinami
authors.forename: M
authors.initials: M
authors.lastname: Itokawa
mesh_terms: D001483:Base Sequence; D004247:DNA; D017931:DNA Primers; D016678:Genome; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D012559:Schizophrenia / Q000235:genetics
chemical_list: D017931:DNA Primers; D017448:Receptors, Dopamine D2; D004247:DNA