Measurement of apolipoproteins A-I and B during the National Health and Nutrition Examination Survey (NHANES) III.
We measured apolipoproteins (apo) A-I and B by rate immunonephelometry (rate INA) during Phase 1 of the National Health and Nutrition Examination Survey (NHANES) III. We also made the measurements by radial immunodiffusion (RID) in a 20% subset of the samples. Aliquots of this subset were also analyzed in the Northwest Lipid Research Laboratories by fixed-time INA calibrated to the World Health Organization (WHO)-International Federation of Clinical Chemistry (IFCC) First International Reference Materials for Apolipoproteins A-I and B. The CVs for the rate INA and RID measurements were: apoA-I, 4.5-7.7% and 2.5-7.6%, respectively; apoB, 2.3-5.3% and 2.3-6.4%, respectively. In NHANES III, rate INA values (x) can be transformed to WHO-IFCC Reference Material-based values (y) as follows: for apoA-I, y = 0.87x + 251.8 mg/L (r = 0.93, SEslope = 0.13, SEintercept = 17, n = 708); for apoB (mg/L), y = 1.068x + 112.8 mg/L (r = 0.98, SEslope = 0.08, SEintercept = 7, n = 646).
Bachorik
P S
PS
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287.
Lovejoy
K L
KL
Carroll
M D
MD
Johnson
C L
CL
Albers
J J
JJ
Marcovina
S M
SM
D016632:Apolipoprotein A-I / Q000032:analysis*; D001055:Apolipoproteins B / Q000032:analysis*; D002624:Chemistry, Clinical / Q000706:statistics & numerical data*; D005612:Freeze Drying; D005615:Freezing; D006306:Health Surveys; D006801:Humans; D007118:Immunoassay / Q000706:statistics & numerical data; D005779:Immunodiffusion / Q000706:statistics & numerical data; D009391:Nephelometry and Turbidimetry / Q000706:statistics & numerical data; D009749:Nutrition Surveys; D011786:Quality Control; D012015:Reference Standards; D012044:Regression Analysis; D012680:Sensitivity and Specificity
D016632:Apolipoprotein A-I; D001055:Apolipoproteins B