The role of nerve growth factor in modulating herpes simplex virus reactivation in vivo.
The role of nerve growth factor (NGF) in the modulation of herpes simplex virus (HSV) latency and reactivation was investigated in a mouse model. To determine whether NGF depletion would reactivate latent virus, concentrated anti-NGF serum antibodies were administered intraperitoneally to latently infected mice for 9 consecutive days. To determine whether NGF given prophylactically could suppress UV-B-induced viral reactivation, mice were irradiated with UV-B while being treated with NGF using diverse regimes over a 4-day period. Following intraperitoneal administration of anti-NGF antibodies, viral shedding was detected in a small number (10%) of mice, but it was not possible to pharmacologically suppress UV-B-induced viral reactivation with NGF. It would appear, therefore, that HSV latency in neurons innervating the cornea can be sustained and disrupted by physiological factors independent of NGF levels.
Laycock
K A
KA
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO 63110.
Brady
R H
RH
Lee
S F
SF
Osborne
P A
PA
Johnson
E M
EM
Pepose
J S
JS
D000818:Animals; D005260:Female; D018259:Herpesvirus 1, Human / Q000254:growth & development* / Q000528:radiation effects; D016849:Keratitis, Herpetic / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009414:Nerve Growth Factors / Q000502:physiology*; D012008:Recurrence; D017783:Superior Cervical Ganglion / Q000382:microbiology / Q000473:pathology; D012668:Trigeminal Ganglion / Q000382:microbiology / Q000473:pathology; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000187:drug effects* / Q000528:radiation effects; D017735:Virus Latency / Q000502:physiology; D017201:Virus Shedding / Q000502:physiology
D009414:Nerve Growth Factors