title: The 2A proteinase of human rhinovirus is a zinc containing enzyme.
abstract: The 2A proteinase of human rhinovirus (HRV) 2 is a cysteine proteinase which is not inhibited by metal chelating agents. However, total hydrolysis of highly purified HRV2 2A followed by atom emission spectroscopy demonstrated that HRV2 2A contains 1 mol of zinc per mole of enzyme. Furthermore, Zn depletion of the enzyme led to a loss of proteolytic activity which could subsequently be restored by Zn supplementation.
authors.affiliation: BENDER+CO Ges. mbH, Department of Cell Biology, Vienna, Austria.
authors.forename: W
authors.initials: W
authors.lastname: Sommergruber
authors.forename: G
authors.initials: G
authors.lastname: Casari
authors.forename: F
authors.initials: F
authors.lastname: Fessl
authors.forename: J
authors.initials: J
authors.lastname: Seipelt
authors.forename: T
authors.initials: T
authors.lastname: Skern
mesh_terms: D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000032:analysis*; D008969:Molecular Sequence Data; D012229:Rhinovirus / Q000201:enzymology*; D014764:Viral Proteins; D015032:Zinc / Q000032:analysis*
chemical_list: D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C057400:picornain 2A, Picornavirus; D015032:Zinc