Neurotrophin-4 selectively promotes survival of striatal neurons in organotypic slice culture.
The neurotrophins (NGF, BDNF, NT-3, and NT-4) provide trophic support to subpopulations of neurons in the central and peripheral nervous systems. We examined organotypic slices of neonatal mouse striatum maintained in medium supplemented with neurotrophins or with CNTF to determine which of these factors influence the survivability of striatal neurons. Neuron counts at the end of the culture period revealed that NT-4 was the only factor that had a significant effect on neuronal survival, suggesting that NT-4 is a trophic factor for striatal neurons in organotypic slices.
Ardelt
A A
AA
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
Flaris
N A
NA
Roth
K A
KA
D000818:Animals; D000831:Animals, Newborn; D019208:Brain-Derived Neurotrophic Factor; D002470:Cell Survival / Q000187:drug effects; D020934:Ciliary Neurotrophic Factor; D051379:Mice; D017072:Neostriatum / Q000166:cytology* / Q000187:drug effects; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D009924:Organ Culture Techniques
D019208:Brain-Derived Neurotrophic Factor; D020934:Ciliary Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; C073922:neurotrophin 4