title: Infection with human cytomegalovirus enhances bacterial adhesiveness and invasiveness in permissive and semipermissive cells.
abstract: The effect of human cytomegalovirus (HCMV) infection on adhesiveness and invasiveness of Salmonella typhimurium was examined in cells permissive (human embryo fibroblasts (HE)), semipermissive (A549) and nonpermissive (HEp-2) for the virus. Preinfection of the cells with HCMV induced enhanced adhesiveness and invasiveness of bacteria in the permissive HE cells. In the semipermissive A549 cells, where HCMV immediate-early (IE) mRNA transcripts and IE proteins were detected, a significant effect on the initial phase of invasiveness, the adherence phase, was demonstrated. HCMV had no effect on invasiveness of S. typhimurium in nonpermissive HEp-2 cells. Neither HCMV IE transcripts nor IE proteins could be detected in these cells.
authors.affiliation: Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Norway.
authors.forename: M
authors.initials: M
authors.lastname: Holberg-Petersen
authors.forename: G
authors.initials: G
authors.lastname: Bukholm
authors.forename: H
authors.initials: H
authors.lastname: Rollag
authors.forename: M
authors.initials: M
authors.lastname: Degré
mesh_terms: D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics; D001422:Bacterial Adhesion; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D003588:Cytopathogenic Effect, Viral; D017931:DNA Primers / Q000737:chemistry; D005347:Fibroblasts / Q000382:microbiology* / Q000821:virology; D005455:Fluorescent Antibody Technique; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics; D007124:Immunoenzyme Techniques; D007822:Laryngeal Neoplasms; D008175:Lung Neoplasms; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012486:Salmonella typhimurium / Q000472:pathogenicity*; D014407:Tumor Cells, Cultured
chemical_list: D000954:Antigens, Surface; D000956:Antigens, Viral; D017931:DNA Primers; D017874:Immediate-Early Proteins; D012333:RNA, Messenger; C044582:immediate-early proteins, cytomegalovirus