title: The RI alpha subunit of protein kinase A controls serum dependency and entry into cell cycle of human mammary epithelial cells.
abstract: MCF-10A, a nontransformed mammary epithelial cell line, requires a mixture of hormones and growth factors for optimal cell proliferation. In this report we show that when MCF-10A cells are cultured in serum free medium they become quiescent and accumulate in G0/G1 phases of the cell cycle. Following addition of complete medium to quiescent cells, MCF-10A cells enter into the S phase within 15-18 h and resume the cell cycle distribution of proliferating cells within 24 h. Measurement of RI alpha subunit of the cAMP-dependent protein kinase (PKA) shows a 10- to 15-fold increase in protein levels at 6 h following complete medium addition, thus preceding cell entry into the S phase. Retroviral vector-mediated overexpression of RI alpha, but not of RII beta or C alpha subunits of PKA, enables MCF-10A cells to grow in serum-free medium. In addition, RI alpha downregulation by specific antisense oligodeoxynucleotide treatment or following infection with a retroviral vector containing the RI alpha cDNA in antisense orientation determines growth arrest of proliferating MCF-10A cells and is able to partially block S phase entry of quiescent MCF-10A cells following complete medium addition. These results suggest that RI alpha/PKAI is involved in the control of cell cycle progression of mammary epithelial cells at a G1 to S transition border, and that its overexpression is able to overcome serum and growth factors requirement for cell proliferation.
authors.affiliation: Cattedra di Oncologia Medica, Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli Federico II, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Tortora
authors.forename: S
authors.initials: S
authors.lastname: Pepe
authors.forename: C
authors.initials: C
authors.lastname: Bianco
authors.forename: G
authors.initials: G
authors.lastname: Baldassarre
authors.forename: A
authors.initials: A
authors.lastname: Budillon
authors.forename: T
authors.initials: T
authors.lastname: Clair
authors.forename: Y S
authors.initials: YS
authors.lastname: Cho-Chung
authors.forename: A R
authors.initials: AR
authors.lastname: Bianco
authors.forename: F
authors.initials: F
authors.lastname: Ciardiello
mesh_terms: D000818:Animals; D001769:Blood; D002453:Cell Cycle; D002460:Cell Line; D003001:Cloning, Molecular; D016895:Culture Media, Serum-Free; D054756:Cyclic AMP-Dependent Protein Kinase RIalpha Subunit; D017868:Cyclic AMP-Dependent Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary; D004848:Epithelium; D005822:Genetic Vectors; D006801:Humans; D008321:Mammary Glands, Animal / Q000166:cytology*; D012190:Retroviridae / Q000235:genetics
chemical_list: D016895:Culture Media, Serum-Free; D054756:Cyclic AMP-Dependent Protein Kinase RIalpha Subunit; D018076:DNA, Complementary; C517834:PRKAR1A protein, human; D017868:Cyclic AMP-Dependent Protein Kinases