title: The phosphorylation of stathmin by MAP kinase.
abstract: Stathmin, a ubiquitous cytosolic phosphoprotein which may play a role in integrating the effects of diverse signals regulating proliferation, differentiation and other cell functions, was found to be phosphorylated rapidly and stoichiometrically by mitogen-activated protein (MAP) kinase in vitro. Ser-25 was identified as the major site and Ser-38 as a minor site of phosphorylation, while the p42 and p44 isoforms of MAP kinase were the only significant stathmin kinases detected in PC12 cells after stimulation by nerve growth factor (NGF). The results suggest that MAP kinases are the enzymes responsible for increasing the level of phosphorylation of Ser-25, which has been observed previously in PC12 cells following stimulation by NGF.
authors.affiliation: Department of Biochemistry, University of Dundee, Scotland, UK.
authors.forename: I A
authors.initials: IA
authors.lastname: Leighton
authors.forename: P
authors.initials: P
authors.lastname: Curmi
authors.forename: D G
authors.initials: DG
authors.lastname: Campbell
authors.forename: P
authors.initials: P
authors.lastname: Cohen
authors.forename: A
authors.initials: A
authors.lastname: Sobel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D007700:Kinetics; D008868:Microtubule Proteins; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology; D016716:PC12 Cells; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010748:Phosphopeptides / Q000737:chemistry / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D012694:Serine; D015398:Signal Transduction; D050777:Stathmin
chemical_list: D008868:Microtubule Proteins; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D010748:Phosphopeptides; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D011994:Recombinant Proteins; D050777:Stathmin; D010768:Phosphoserine; D012694:Serine; D017871:Calcium-Calmodulin-Dependent Protein Kinases