Effect of parathyroid hormone-related peptide supplementation of soy protein formulas in the neonatal pig model.
PTH-related peptide (PTHrP) is found in all milks, including human and pig. To define a role for PTHrP in milk, 2-day-old piglets were randomized to receive soy formula devoid of PTHrP or supplemented with 1 nM synthetic PTHrP(1-86) (n = 8 per group). The number of serum samples with detectable PTHrP by immunoassay (Incstar) and radiometric assay (Nichols) was 9 of 33 and 3 of 13 in PTHrP- and 8 of 27 and 3 of 15 in PTHrP+ formula-fed piglets and 8 of 14 and 7 of 12 in naturally suckling piglets, respectively. Serum and urine concentrations of calcium and magnesium and total and bone alkaline phosphatase were similar in both groups at 3, 6, 10, and 17 days of age. No differences were seen in bone mineral content of the tibia measured by single-photon absorptiometry (BMC 0.22 +/- 0.06 and 0.22 +/- 0.10) or dual x-ray absorption (BMC 1.43 +/- 0.36 and 1.31 +/- 0.78) either in vivo or on excised bone or by measurement of Ca, Mg, or P content or total bone ash (1.26 +/- 0.26 and 1.38 +/- 0.28 mg). Intestinal histology, serum intestinal alkaline phosphatase, and net absorption and retention of Ca, Mg, and P in balances from age 11-17 days were all similar. As in humans, however, a developmental pattern was seen for phosphorus regulation in both groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Hillman
L S
LS
University of Missouri School of Medicine, Department of Child Health, Columbia.
Forte
L R
LR
Veum
T
T
Ru
Y
Y
Howard
B
B
Rowe
A
A
Hillman
H W
HW
D015502:Absorptiometry, Photon; D000469:Alkaline Phosphatase / Q000097:blood / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D015519:Bone Density / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D004032:Diet; D005527:Food, Fortified; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D008274:Magnesium / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D010455:Peptides / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D010758:Phosphorus / Q000378:metabolism*; D014674:Plant Proteins, Dietary / Q000008:administration & dosage; D011897:Random Allocation; D030262:Soybean Proteins; D013552:Swine; D013977:Tibia / Q000187:drug effects
D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D010455:Peptides; D014674:Plant Proteins, Dietary; D030262:Soybean Proteins; C068871:parathyroid hormone-related protein (1-86); D010758:Phosphorus; D000469:Alkaline Phosphatase; D008274:Magnesium; D002118:Calcium