title: Allergenic cross-reactivity among peach, apricot, plum, and cherry in patients with oral allergy syndrome: an in vivo and in vitro study.
abstract: BACKGROUND
  Oral allergy syndrome in response to fruits and vegetables frequently occurs as clusters of hypersensitivity to members of the same botanical family, for which the immunologic basis lies in a number of common allergens, most of them still unidentified.
  
  
  OBJECTIVE
  This study was designed to assess the in vivo and in vitro cross-reactivity between fruits of the Prunoideae subfamily (i.e., peach, cherry, apricot, and plum) and to identify their major allergens and the cross-reactivity of the peach extract with grass and birch pollen.
  
  
  METHODS
  The in vivo study was conducted by skin prick tests and open food challenges with fresh fruits in 23 patients with oral allergy syndrome for peach and positive skin prick test and RAST results for the other Prunoideae. In vitro sodium dodecylsulfate-polyacrylamide gel electrophoresis was followed by immunoblotting and immunoblotting-inhibition.
  
  
  RESULTS
  A 13 kd component was identified as the only major allergen common to all the Prunoideae, the other major allergens were found at 14 kd in peach and at 30 kd in cherry. Immunoblotting inhibition showed wide cross-reactivity within the Prunoideae, whereas grass and birch pollen partially inhibited the peach blotting.
  
  
  CONCLUSIONS
  Clinical cross-reactivity to Prunoideae is essentially due to a common 13 kd IgE-binding component, which seems to be the most important major allergen of this subfamily, not shared with grass and birch pollen.
authors.affiliation: First Department of Internal Medicine, University of Milan, Italy.
authors.forename: E A
authors.initials: EA
authors.lastname: Pastorello
authors.forename: C
authors.initials: C
authors.lastname: Ortolani
authors.forename: L
authors.initials: L
authors.lastname: Farioli
authors.forename: V
authors.initials: V
authors.lastname: Pravettoni
authors.forename: M
authors.initials: M
authors.lastname: Ispano
authors.forename: A
authors.initials: A
authors.lastname: Borga
authors.forename: A
authors.initials: A
authors.lastname: Bengtsson
authors.forename: C
authors.initials: C
authors.lastname: Incorvaia
authors.forename: C
authors.initials: C
authors.lastname: Berti
authors.forename: C
authors.initials: C
authors.lastname: Zanussi
mesh_terms: D000485:Allergens / Q000276:immunology*; D003429:Cross Reactions; D005512:Food Hypersensitivity / Q000276:immunology*; D005638:Fruit / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology; D010936:Plant Extracts / Q000276:immunology; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000276:immunology; D012882:Skin Tests; D013577:Syndrome; D014197:Trees / Q000276:immunology
chemical_list: D000485:Allergens; D010936:Plant Extracts; D007073:Immunoglobulin E