title: [Proteinases with various substrate specificities in structural studies of yeast cell walls].
abstract: Different proteins are revealed in cell wall of yeast cells Candida utilis by means of specific proteolysis with subtilisins TV and 72, trypsin and purified collagenase of Clostridium histolyticum. Some of them were characterized by resistance to trypsin and sensitivity to subtilisin TV. In young cells this group is represented essentially by a protein of 33 kD, which appears to be one of the structural proteins, binding fibrillae of carbohydrate. Other proteins proved to be sensitive to both trypsin and subtilisin. Among these proteins a protein with mol. mass 80 kD was revealed; its sensitivity to extremely specific hydrolysis by bacterial collagenase suggests it to contain amino acid sequences characteristic for collagens of higher eukaryotes.
authors.forename: T S
authors.initials: TS
authors.lastname: Kalebina
authors.forename: M V
authors.initials: MV
authors.lastname: Nurminskaia
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Chertov
authors.forename: G N
authors.initials: GN
authors.lastname: Rudenskaia
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
authors.forename: I S
authors.initials: IS
authors.lastname: Kulaev
mesh_terms: D000595:Amino Acid Sequence; D002175:Candida / Q000201:enzymology*; D002473:Cell Wall / Q000737:chemistry / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: D010450:Endopeptidases
vernacular_title: Proteinazy s razlichnoĭ substratnoĭ spetsifichnost'iu v strukturnykh issledovaniiakh kletochnoĭ stenki drozhzheĭ.