Lymphatic absorption of n - 3 polyunsaturated fatty acids from marine oils with different intramolecular fatty acid distributions.
Male Wistar rats were given 0.5 ml of either fish oil or seal oil intragastrically. The intramolecular fatty acid distributions of the triacylglycerols administered were determined by non-specific Grignard degradation followed by isolation and analysis of the 2-monoacylglycerols. The n - 3 polyunsaturated fatty acids (PUFAs), especially eicosapentaenoic acid (20:5(n - 3)) and docosahexaenoic acid (22:6(n - 3)), were located in outer positions (sn-1/3) in the seal oil triacylglycerols whereas the sn-2 position of fish oil triacylglycerols was enriched in 20:5(n - 3) and 22:6(n - 3). The mesenteric lymph was collected over the following 24 h and the absorption patterns of n-3 PUFAs were determined. In the lymph, the n - 3 fatty acids characteristic of the marine oils rapidly increased both with regard to mole percentage and transport (micrograms/min). There were, however, no overall significant differences in the absorption patterns over a 24 h period. The ratio between mole percentage in the oil and mole percentage in the lymph calculated at the steady-state period was significantly greater for both 20:5(n - 3) and 22:6(n - 3) following fish oil administration compared with seal oil. Initially, the recovery of n - 3 PUFAs as a percentage of the total amount transported over the experimental period was higher following injection of fish oil than seal oil but seal oil resulted in greater recovery in the last two fractions at 8 and 24 h post injection, respectively. This indicated that n - 3 PUFAs from fish oil may have been better absorbed in the initial period of digestion but overall the structure of dietary triacylglycerols had negligible effects on the assimilation of n - 3 PUFAs when these were administered as native marine oils.
Christensen
M S
MS
Department of Biochemistry and Nutrition, Technical University of Denmark, Lyngby.
Høy
C E
CE
Redgrave
T G
TG
D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage; D005227:Fatty Acids / Q000032:analysis; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D005395:Fish Oils / Q000008:administration & dosage* / Q000737:chemistry; D008208:Lymphatic System / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D046048:Seals, Earless; D014280:Triglycerides / Q000737:chemistry
D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D014280:Triglycerides