Comparisons of repetitive-and single-insult ischaemia: effects on regional brain damage and behaviour.
Single- and repetitive-insult ischaemia have been used with the gerbil to model human stroke and the impact of anti-ischaemic agents. It is currently not well-established whether the duration of an ischaemic insult, or single vs repetitive insults may have different behavioural consequences. In this study, groups of gerbils were given either single- or repetitive-insult ischaemia of either 3, 6 or 9 min cumulative duration. As the cumulative duration of ischaemia was increased, the degree of brain damage correspondingly increased and performance in a water maze task was progressively more impaired. However, no behavioural or brain-damage differences were found between groups of animals which received comparable durations of single- or repetitive-insult ischaemia. Significant mortality was observed in the group receiving three 3 min ischaemic insults, suggesting that the repetitive ischaemia technique may be limited to brief periods of occlusion. We conclude that there are no grounds for distinguishing between the single- and repetitive-ischaemia paradigms when employing cumulative durations up to 9 min.
Wishart
T
T
Department of Psychology, University of Saskatchewan, Saskatoon, Canada.
McCrea
S
S
Ijaz
S
S
Shuaib
A
A
D000818:Animals; D001921:Brain / Q000473:pathology*; D003643:Death; D005849:Gerbillinae; D002546:Ischemic Attack, Transient / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D018782:Maze Learning; D009928:Organ Specificity; D016548:Prosencephalon / Q000473:pathology; D012008:Recurrence; D013997:Time Factors