title: Use of an ELISA test in the eradication of an equine infectious anaemia focus.
abstract: An enzyme linked immunosorbent assay (ELISA) test and the classic agar gel immunodiffusion (AGID) test were used as diagnostic methods in the eradication of a focus of equine infectious anaemia from a herd of 86 horses. The ELISA test proved to be more sensitive, detecting positive animals earlier than the AGID test. A group of 16 animals positive only by ELISA also became positive to the AGID when retested one month later, except for 2 animals which showed clinical signs of the disease and died before retesting.
authors.affiliation: Department of Veterinary Preventive Medicine, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: J K
authors.initials: JK
authors.lastname: dos Reis
authors.forename: L M
authors.initials: LM
authors.lastname: Melo
authors.forename: M R
authors.initials: MR
authors.lastname: Rezende
authors.forename: R C
authors.initials: RC
authors.lastname: Leite
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D004859:Equine Infectious Anemia / Q000097:blood / Q000175:diagnosis*; D006736:Horses; D005779:Immunodiffusion / Q000662:veterinary; D004860:Infectious Anemia Virus, Equine / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral