title: Determination of methyl 2-benzimidazolecarbamate in bulk fruit juice concentrates by competitive-inhibition enzyme immunoassay.
abstract: A polyclonal enzyme immunoassay (EIA) was used to quantitate methyl 2-benzimidazolecarbamate (MBC or carbendazim), a degradation product of benomyl, in bulk fruit juice concentrates. These concentrates are used by industrial producers to prepare juice or juice concentrates sold in supermarkets. Total sample analysis time was less than 18 min without cleanup or 35 min with cleanup. As many as 8 samples can be analyzed simultaneously, with a limit of quantitation of 10 ppb. The assay's dynamic range ran from 0.5 to 20 ppb MBC but was best from 0.5 to 10 ppb. Intra-assay coefficients of variation (CVs) varied from 4.0 to 13% for standards and from 4.1 to 26% for samples. Interassay CVs varied from 4.5 to 47% for standards and 5.6 to 22% for samples. Average recovery of several juice concentrates spiked at 10 to 290 ppb was 97%. A total of 140 juice concentrates comprising 20 different kinds of juice were analyzed by 2 EIA methods and one liquid chromatographic (LC) procedure. MBC-positive samples gave the following correlation coefficients: 0.954 for EIA without cleanup vs LC, 0.956 for EIA with cleanup vs LC, and 0.978 for EIA with cleanup vs EIA without cleanup. MBC concentrations in MBC-positive juice samples ranged from 5 to 2960 ppb.
authors.affiliation: University of Maine, Department of Food Science, Orono 04469-5736.
authors.forename: R J
authors.initials: RJ
authors.lastname: Bushway
authors.forename: B E
authors.initials: BE
authors.lastname: Young
authors.forename: L R
authors.initials: LR
authors.lastname: Paradis
authors.forename: L B
authors.initials: LB
authors.lastname: Perkins
authors.forename: S K
authors.initials: SK
authors.lastname: Martin
authors.forename: M P
authors.initials: MP
authors.lastname: Brown
mesh_terms: D001562:Benzimidazoles / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D002219:Carbamates; D005506:Food Contamination; D005638:Fruit; D005659:Fungicides, Industrial / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001562:Benzimidazoles; D002219:Carbamates; D005659:Fungicides, Industrial; C006698:carbendazim