Comparative investigation of the effects of zardaverine and theophylline on pulmonary function in rats.
Zardaverine (Byk Gulden, Konstanz, Germany) is a new selective phosphodiesterase (PDE) III/IV inhibitor. The bronchodilating and bronchoprotective potency of zardaverine and the nonselective PDE inhibitor theophylline was compared by measuring typical spontaneous and forced respiratory function parameters in anesthetized female Wistar rats using whole-body plethysmography. Zardaverine (3, 10, 30 mumol/kg) and theophylline (30, 100, 300 mumol/kg), respectively, were given orally in 4% Methocel/0.9% saline solution 20 min before measurement. One week before treatment, control measurements were performed in the same animals after administration of the vehicle. When spontaneously breathing, the 30 mumol/kg zardaverine- (300 mumol/kg theophylline-) treated animals showed significant changes: a 23% (14% ns) decrease in lung resistance and a 43% (25%) increase in dynamic compliance. These changes can be interpreted as an indication of bronchodilation, since quasistatic lung compliance was unchanged. In the acetylcholine challenge test, treatment with only 10 mumol/kg zardaverine (but 300 mumol/kg theophylline) revealed significant changes compared to control measurement: a 37% (28%) lower resistance and 85% (44%) higher compliance. It has been determined that zardaverine is more than 30 times as potent as theophylline in inhibiting acetylcholine-induced bronchospasms, which is also supported by forced expiratory flow data.
Hoymann
H G
HG
Fraunhofer Institute of Toxicology and Aerosol Research, Hannover, Germany.
Heinrich
U
U
Beume
R
R
Kilian
U
U
D000818:Animals; D001993:Bronchodilator Agents / Q000494:pharmacology*; D005260:Female; D008168:Lung / Q000187:drug effects*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010993:Plethysmography, Whole Body; D011724:Pyridazines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012129:Respiratory Function Tests; D013806:Theophylline / Q000494:pharmacology*
D001993:Bronchodilator Agents; D010726:Phosphodiesterase Inhibitors; D011724:Pyridazines; D013806:Theophylline; C066436:zardaverine