title: Effects of perfusion rate and glucose concentration on glucose absorption in intestinal thiry-vella loop in sheep.
abstract: The effects of the perfusion rate and glucose concentration in perfusate on glucose absorption in the intestinal Thiry-Vella loop of conscious sheep were studied. When 10 mM glucose was perfused at the rates of 0.5, 1, 2 and 4 ml/min, the absorption rates were 77.1 +/- 2.2, 54.9 +/- 2.3, 31.6 +/- 2.1 and 12.9 +/- 0.3%, respectively. When 5, 10, 20 and 40 mM glucose solutions were perfused at 1 ml/min, the absorption rates were 75.5 +/- 1.7, 52.2 +/- 2.2, 29.4 +/- 1.9 and 19.5 +/- 0.1%, respectively. We conclude that the perfusion of 10 mM glucose solution at 1 ml/min, in which about a 50% absorption rate was obtained, are the optimal conditions for the study of glucose absorption in the intestinal loop of sheep.
authors.affiliation: Department of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: H S
authors.initials: HS
authors.lastname: Hyun
authors.forename: T
authors.initials: T
authors.lastname: Onaga
authors.forename: H
authors.initials: H
authors.lastname: Mineo
authors.forename: S
authors.initials: S
authors.lastname: Kato
mesh_terms: D000818:Animals; D005947:Glucose / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007408:Intestinal Absorption; D007700:Kinetics; D008297:Male; D010477:Perfusion / Q000295:instrumentation* / Q000379:methods; D012756:Sheep; D013997:Time Factors
chemical_list: D005947:Glucose