title: Effects of prostaglandins on [Ca2+]i and adenylate cyclase activity in isolated porcine gastric mucous cells.
abstract: In porcine gastric mucous cells, isolated enzymatically from the fundic mucosa and enriched by counterflow centrifugation, PGE2 (1 microM) increased adenylate cyclase activity to 225% and, distinct from that documented for other species, also [Ca2+]i, measured fluorimetrically with Fura2/AM, in Ca(2+)-containing and Ca(2+)-free incubation medium to 182% and 165% of control values, respectively. PGF2 alpha, PGD2, the stable prostacyclin analogue iloprost and the thromboxane-mimetic U46619 had no significant effects on adenylate cyclase activity and [Ca2+]i. Histamine (10 microM) stimulated adenylate cyclase activity to 236% of control value, an effect which could be blocked by the H2-receptor antagonist ranitidine. However, histamine and the activators of the cAMP system forskolin and dibutyryl cAMP had no significant effect on [Ca2+]i, indicating that an activation of the adenylate cyclase/cAMP system per se does not result in an increase in [Ca2+]i. These data suggest that prostanoids stimulate adenylate cyclase activity and [Ca2+]i in gastric mucous cells via activation of EP-receptors linked to both second messenger systems.
authors.affiliation: Institute of General Pharmacology, Medical School Hannover, FRG.
authors.forename: H K
authors.initials: HK
authors.lastname: Heim
authors.forename: R I
authors.initials: RI
authors.lastname: Bersimbaev
authors.forename: A
authors.initials: A
authors.lastname: Gandjour
authors.forename: W
authors.initials: W
authors.lastname: Beil
authors.forename: K F
authors.initials: KF
authors.lastname: Sewing
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D011899:Ranitidine / Q000494:pharmacology; D013552:Swine
chemical_list: D011453:Prostaglandins; D011506:Proteins; D005576:Colforsin; D003994:Bucladesine; D001285:Atropine; D006632:Histamine; D011899:Ranitidine; D002217:Carbachol; D000262:Adenylyl Cyclases; D002118:Calcium