Measurement of cell migration stimulated by interleukin 8: use of ATP chemiluminescence.
The reliability of methods for the measurement of chemotaxis is a problem which many previous studies have attempted to solve. However, it has recently been shown that the number of cells migrating through a filter can be measured by determining their ATP content by chemiluminescence. We have adapted this method to use microplates with a 24-well format. This allows cells traversing the membranes to be harvested by centrifugation. The modified method has been used to measure the chemotactic activity of interleukin 8 (IL-8) for human polymorphonuclear leukocytes (PMNL). There was a dose response to IL-8, with an optimal concentration of 10(-8) micrograms/ml. The main advantages of this ATP-based cell migration assay were found to be its speed, reproducibility and ease of use.
McCafferty
A C
AC
Department of Pathology, University of Dundee, Ninewells Hospital, Scotland.
Cree
I A
IA
D000255:Adenosine Triphosphate / Q000032:analysis*; D000328:Adult; D002465:Cell Movement / Q000187:drug effects*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D008163:Luminescent Measurements; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects*; D013268:Stimulation, Chemical
D016209:Interleukin-8; D000255:Adenosine Triphosphate