Effect of cytokine-induced soluble ICAM-1 from human synovial cells on synovial cell-lymphocyte adhesion.
The present study was designed to establish (i) the effects of cytokines on soluble ICAM-1 (sICAM-1) production by human synovial cells (SC) and ICAM-1 expression on these cells, and (ii) the effects of sICAM-1 on lymphocyte-SC adhesion. sICAM-1 production was enhanced in parallel with ICAM-1 expression by IL-1 beta, TNF-alpha and IFN-gamma. IL-4 showed no effects on ICAM-1 expression. In contrast with the transient elevation of cell-associated ICAM-1 by IL-1 beta, which peaked 36 h after stimulation and declined thereafter, sICAM-1 continued to accumulate in culture supernatants even after 48 h. Purified sICAM-1 was obtained from a 48 h culture synovial cell supernatant by affinity chromatography using ICAM-1 monoclonal antibody. The purified sICAM-1 significantly inhibited adhesion of lymphocytes and monocytes to cytokine-stimulated synovial cells. These results suggest that sICAM-1 may modulate chronic synovitis by inhibiting ICAM-1-mediated cell-to-cell adhesion.
Shingu
M
M
Department of Clinical Immunology, Kyushu University, Beppu, Japan.
Hashimoto
M
M
Ezaki
I
I
Nobunaga
M
M
D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D007375:Interleukin-1 / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology; D013583:Synovial Membrane / Q000166:cytology / Q000276:immunology*
D017077:Culture Media, Conditioned; D016207:Cytokines; D007375:Interleukin-1; D018799:Intercellular Adhesion Molecule-1