title: Dehydration and ABA increase mRNA levels and enzyme activity of cytosolic GAPDH in the resurrection plant Craterostigma plantagineum.
abstract: We report the identification of a full-length cDNA clone encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) in the desiccation-tolerant plant Craterostigma plantagineum. The DNA sequence of the cDNA clone is homologous to cytosolic GAPDH cDNAs from other higher plants. The GAPDH transcript increases rapidly in abundance during dehydration or abscisic acid (ABA) treatment. The increase in mRNA levels is directly correlated with higher protein and enzyme levels. These results imply that enhanced rates of glycolysis are one of the immediate cellular responses to water deficit. This may be a mechanism by which the plant cell prepares for a demand of ATP and NADH2 during recovery.
authors.affiliation: Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Velasco
authors.forename: F
authors.initials: F
authors.lastname: Salamini
authors.forename: D
authors.initials: D
authors.lastname: Bartels
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D003890:Desiccation; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D015723:Gene Library; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D018749:RNA, Plant / Q000032:analysis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D018749:RNA, Plant; D000040:Abscisic Acid; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases