Effects of a fish-oil and vegetable-oil formula on aggregation and ethanolamine-containing lysophospholipid generation in activated human platelets and on leukotriene production in stimulated neutrophils.
The effects of consuming a liquid formula containing either fish oil enriched in omega-3 fatty acids or vegetable oil enriched in oleic acid was evaluated in 20 male subjects randomly allocated into two groups over a 42-d period. A decrease in collagen-induced aggregation by using washed platelet suspensions was found in both groups after nutritional supplementation. A considerable rise in omega-3 and a decrease in omega-6 fatty acids occurred in the platelet phospholipid with fish-oil consumption. The degree of eicosapentaenoic acid (EPA, 20:5n-3) enrichment (fish-oil group) was dramatically greater in the ether-containing plasmenylethanolamine (13.5 mol% of fatty acids; mol% of fatty acids = moles per 100 moles of total fatty acids) than in phosphatidylethanolamine (2.8 mol%) or phosphatidylcholine (2.9 mol%). Neither treatment significantly influenced the agonist-induced accumulation of lysoplasmenylethanolamine as derived via phospholipase A2 hydrolysis of plasmenylethanolamine. HPLC measurements of eicosanoid production in A23187-stimulated neutrophils revealed a considerable decrease in the formation of arachidonic acid-derived leukotriene B4 (LTB4), by 41%, and 5-HETE (5-hydroxyeicosatetraenoic acid), by 30%, in the fish-oil group along with the appearance of the corresponding EPA-derived products [LTB5 and 5-HEPE (5-hydroxyeicosapentaenoic acid)]. No such alterations in the formation of lipoxygenase products were found with the vegetable oil treatment.
Turini
M E
ME
Department of Nutritional Sciences, University of Guelph, Ontario, Canada.
Powell
W S
WS
Behr
S R
SR
Holub
B J
BJ
D000328:Adult; D001773:Blood Cells / Q000187:drug effects*; D001792:Blood Platelets / Q000378:metabolism; D019856:Ethanolamine; D004983:Ethanolamines / Q000097:blood; D005395:Fish Oils / Q000494:pharmacology*; D005526:Food, Formulated; D006801:Humans; D015289:Leukotrienes / Q000096:biosynthesis*; D008246:Lysophospholipids / Q000096:biosynthesis*; D008297:Male; D018375:Neutrophil Activation; D009504:Neutrophils / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology*; D015539:Platelet Activation; D010974:Platelet Aggregation / Q000187:drug effects
D004983:Ethanolamines; D005395:Fish Oils; D015289:Leukotrienes; D008246:Lysophospholipids; D010938:Plant Oils; D019856:Ethanolamine