Identification of genes differentially expressed in normal lung and non-small cell lung carcinoma tissue.
Using a magnet-assisted subtraction technique, 17 complementary DNA (cDNA) clones were isolated that were expressed in the normal lung but were decreased or lost in the corresponding tumor tissue of a nonsmall cell lung carcinoma patient. The lack of expression of six magnet-assisted subtraction technique cDNA clones in three additional non-small cell lung carcinoma cases indicates their possible relevance for non-small cell lung carcinoma. Two cDNA clones revealed homology to genes specifically expressed in lung, i.e., pulmonary surfactant-associated protein B and the receptor for advanced glycosylation end products of proteins. Three cDNA clones showed identity to cDNA sequences encoding calmodulin-like protein, glutamine synthetase, and cytoskeletal beta-actin. One cDNA clone is identical to a recently described human expressed sequence tag whose gene is still unknown.
Schraml
P
P
Kantonsspital Basel, Zentrum für Lehre und Forschung, Switzerland.
Shipman
R
R
Colombi
M
M
Ludwig
C U
CU
D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification*; D016326:Extracellular Matrix Proteins; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008175:Lung Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D011510:Proteolipids / Q000235:genetics; D011663:Pulmonary Surfactants / Q000235:genetics; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000235:genetics; D013382:Subtraction Technique
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D011510:Proteolipids; D011663:Pulmonary Surfactants; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; C503126:SPARCL1 protein, human