Biodegradation of 2-nitrotoluene by Pseudomonas sp. strain JS42.
A strain of Pseudomonas sp. was isolated from nitrobenzene-contaminated soil and groundwater on 2-nitrotoluene as the sole source of carbon, energy, and nitrogen. Bacterial cells growing on 2-nitrotoluene released nitrite into the growth medium. The isolate also grew on 3-methylcatechol, 4-methylcatechol, and catechol. 2-Nitrotoluene, 3-methylcatechol, and catechol stimulated oxygen consumption by intact cells regardless of the growth substrate. Crude extracts from the isolate contained catechol 2,3-dioxygenase and 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase activity. The results suggest that 2-nitrotoluene is subject to initial attack by a dioxygenase enzyme that forms 3-methylcatechol with concomitant release of nitrite. The 3-methylcatechol is subsequently degraded via the meta ring fission pathway.
Haigler
B E
BE
Armstrong Laboratory, EQC-OL, Tyndall Air Force Base, Florida 32403-5319.
Wallace
W H
WH
Spain
J C
JC
D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase; D049308:Dioxygenases; D018698:Glutamic Acid / Q000378:metabolism; D008956:Models, Chemical; D010105:Oxygenases / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D014050:Toluene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D014871:Water Microbiology
C029956:3-nitrotoluene; D014050:Toluene; D018698:Glutamic Acid; C029955:2-nitrotoluene; C017572:4-nitrotoluene; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase