title: Yta10p is required for the ATP-dependent degradation of polypeptides in the inner membrane of mitochondria.
abstract: Incompletely synthesized polypeptides in the mitochondrial inner membrane are subject to rapid proteolysis. We demonstrate that Yta10p, a mitochondrial homologue of a conserved family of putative ATPases in Saccharomyces cerevisiae, is essential for this proteolytic process. Yta10p-dependent degradation requires divalent metal ions and the hydrolysis of ATP. Yta10p is an integral protein of the inner mitochondrial membrane exposing the carboxy terminus to the mitochondrial matrix space. Based on the presence of consensus binding sites for ATP, and for divalent metal ions found in a number of metal dependent endopeptidases, a direct role of Yta10p in the proteolytic breakdown of membrane-associated polypeptides in mitochondria is suggested.
authors.affiliation: Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Pajic
authors.forename: R
authors.initials: R
authors.lastname: Tauer
authors.forename: H
authors.initials: H
authors.lastname: Feldmann
authors.forename: W
authors.initials: W
authors.lastname: Neupert
authors.forename: T
authors.initials: T
authors.lastname: Langer
mesh_terms: D000251:Adenosine Triphosphatases; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology*; D001665:Binding Sites; D002413:Cations, Divalent; D005656:Fungal Proteins / Q000378:metabolism*; D006868:Hydrolysis; D007425:Intracellular Membranes / Q000201:enzymology*; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure; D010455:Peptides / Q000378:metabolism*; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D002413:Cations, Divalent; D005656:Fungal Proteins; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C090045:AFG3 protein, S cerevisiae