In vitro selective toxicity of toxin T-514 from Karwinskia humboldtiana (buckthorn) plant on various human tumor cell lines.
Toxin T-514 is a dimeric anthracenone isolated from the Karwinskia humboldtiana (buckthorn) plant. Its potential anti-neoplastic effect was evaluated in vitro and the results obtained were compared with the effect of other known anti-cancer agents. Normal and malignant continuous cell lines were tested. After a 72-h exposure, neoplastic cells derived from hepatic, pulmonary and colonic tissues were more sensitive to toxin T-514 than normal cells from the corresponding organ. Hepatoma cells and colon adenocarcinoma CT50 values were < 10 micrograms/ml. Lung adenocarcinoma, undifferentiated bronchogenic cancer cells and small cell carcinoma CT50 values were < 20 micrograms/ml. All benign cell CT50 levels tested were > 113 micrograms/ml. This in vitro selective toxicity found with toxin T-514 was also seen with 5-fluororacil and mitomycin for colon adenocarcinoma and with epidoxorubicin for undifferentiated bronchogenic cancer cells.
Piñeyro-López
A
A
Departamento de Farmacología y Toxicología, Facultad de Medicina, Universidad Autónoma de Nuevo León, México.
Martínez de Villarreal
L
L
González-Alanís
R
R
D000873:Anthracenes / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D003106:Colon / Q000166:cytology; D003110:Colonic Neoplasms / Q000188:drug therapy; D006801:Humans; D008099:Liver / Q000166:cytology; D008113:Liver Neoplasms / Q000188:drug therapy; D008168:Lung / Q000166:cytology; D008175:Lung Neoplasms / Q000188:drug therapy; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000873:Anthracenes; D000972:Antineoplastic Agents, Phytogenic; C075149:Karwinskia toxin T-514