Ganglioside antigen of DU-PAN-2 in a human pancreatic cancer.
DU-PAN-2 reactive gangliosides were isolated from the tumor of a patient with pancreatic cancer (duct cell carcinoma, moderately differentiated adenocarcinoma), having a negative Lewis blood phenotype, and were analyzed by means of TLC-immunostaining, enzyme-linked immunosorbent assay (ELISA), permethylation study, 1H NMR spectroscopy and fast atom bombardment mass spectrometry. The structures of the gangliosides were found to be NeuAc alpha 2-3Gal beta 1-3GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1'Cer, containing normal and hydroxy fatty acids. By TLC-immunostaining and ELISA with chemically synthesized gangliosides, DU-PAN-2 was demonstrated to react strongly with IV3 alpha NeuAc-Lc4Cer, weakly with IV3 alpha NeuAc-nLc4Cer, and moderately with IV6 alpha NeuAc-Lc4Cer and IV6 alpha NeuAc-nLc4Cer. Thus it was concluded that the DU-PAN-2 reactive ganglioside in the tumor is IV3 alpha NeuAc-Lc4Cer and that DU-PAN-2 has a rather broad specificity.
Hamanaka
Y
Y
Second Department of Surgery, Yamaguchi University School of Medicine, Japan.
Hamanaka
S
S
Shinagawa
Y
Y
Suzuki
T
T
Inagaki
F
F
Suzuki
M
M
Suzuki
A
A
D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D002240:Carbohydrate Sequence; D002518:Ceramides / Q000276:immunology; D005260:Female; D005732:Gangliosides / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D010190:Pancreatic Neoplasms / Q000276:immunology*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D002518:Ceramides; D005732:Gangliosides