Characterization of lymphocyte subpopulations in cord blood.
Twenty-seven cord blood samples from healthy newborns were processed according to a "whole blood" flow cytometric analysis. The CD3-positive T cells were a variable subpopulation representing 44.8 +/- 13.3% of lymphocytes. The majority of the CD3+ cells are CD38+. Newborn T cells have lower levels of both IL-2 receptors and HLA-DR than do adult T cells. The CD4-positive T cells represented 31.0 +/- 10.8% of lymphocytes with a great prevalence of the CD4+/CD45RA+ population. The CD3+/CD8+/CD11b+ cells are increased to 23.4 +/- 7.1% of lymphocytes. The CD57 antigen is not expressed. The NK population, CD16+/CD56+, is increased to 25 +/- 11% of lymphocytes. Of CD19+ cord blood B lymphocytes 68% coexpressed CD5. Thus "suppressive" and "naive" cells are prominently represented in cord blood.
Rabian-Herzog
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C
Laboratory of Immunology and Histocompatibility, Hôpital Saint-Louis, Paris, France.
Lesage
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S
Gluckman
E
E
Charron
D
D
D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D016131:Lymphocyte Subsets / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000032:analysis; D050378:T-Lymphocytes, Regulatory
D015703:Antigens, CD; D015415:Biomarkers; D006684:HLA-DR Antigens; D015375:Receptors, Interleukin-2