title: Reversed-phase high-performance liquid chromatography for the determination of haemorphin-like immunoreactivity in human cerebrospinal fluid.
abstract: The haemorphins are opioid peptides derived form the blood protein haemoglobin. This study was focused on the detection and determination of haemorphin-like immunoreactivity in human cerebrospinal fluid (CSF) by reversed-phase HPLC. For this purpose a SMART System, optimized for micropurification, was applied. Prior to application to HPLC, the peptide fraction of the CSF sample was extracted using a reversed-phase silica gel cartridge (Sep-Pak C18). In the HPLC separation, the peptide-like material associated with haemorphin-7 immunoreactivity was recovered and determined using a UV detector. The tryptophan residue present in the haemorphin sequence allowed UV detection at wavelengths (e.g., 276 nm) where interference with other co-eluting peptides lacking this residue is minimized. Recorded levels of haemorphin-like immunoreactivity were compared with those detected by radioimmunoassay.
authors.affiliation: Department of Pharmaceutical Biosciences, Uppsala University, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Sanderson
authors.forename: M
authors.initials: M
authors.lastname: Thörnwall
authors.forename: G
authors.initials: G
authors.lastname: Nyberg
authors.forename: E L
authors.initials: EL
authors.lastname: Glämsta
authors.forename: F
authors.initials: F
authors.lastname: Nyberg
mesh_terms: D000595:Amino Acid Sequence; D002543:Cerebral Hemorrhage / Q000134:cerebrospinal fluid; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006408:Hematoma, Subdural / Q000134:cerebrospinal fluid; D006454:Hemoglobins / Q000134:cerebrospinal fluid* / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D013345:Subarachnoid Hemorrhage / Q000134:cerebrospinal fluid
chemical_list: D006454:Hemoglobins