title: Distinct intracellular lysozyme content in normal granulocytes and monocytes: a quantitative immunoperoxidase and ultrastructural immunogold study.
abstract: Using two different immunological methods, we performed a quantitative estimation of lysozyme (LZ) in normal mature granulocytes and monocytes. An immunoperoxidase reaction for LZ in granulocytes and monocytes of 10 healthy donors measured by a scanning microdensitometer as arbitrary units showed a significantly higher LZ content in granulocytes than in monocytes. An ultrastructural immunogold reaction (IGR) for LZ performed on post-embedded thin sections showed a higher number of total gold grains in neutrophilic granulocytes than in monocytes. In monocytic granules we found a high density of gold grains per micron 2, whereas in granulocytic granules lower values were obtained. In granulocytes, LZ was found in both primary myeloperoxidase (MPO)-positive and secondary MPO-negative granules, and in monocytes the granules showed weak MPO reactivity and high LZ content. Granulocytes previously subjected to phagocytosis of bacteria and latex particles showed release of LZ on degranulation inside the phagosome, whereas in monocytes the granules remained outside the phagosome and released LZ without degranulation. Our study demonstrated a significantly higher total LZ content in granulocytes, a higher granular LZ content in monocytes, and release of LZ from intact monocyte granules during phagocytosis.
authors.affiliation: Department of Medicine B, Kaplan Hospital, Rehovot, Israel.
authors.forename: P
authors.initials: P
authors.lastname: Resnitzky
authors.forename: D
authors.initials: D
authors.lastname: Shaft
authors.forename: A
authors.initials: A
authors.lastname: Yaari
authors.forename: E
authors.initials: E
authors.lastname: Nir
mesh_terms: D003720:Densitometry; D005260:Female; D006098:Granulocytes / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D007840:Latex; D008297:Male; D016253:Microscopy, Immunoelectron; D009000:Monocytes / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D009113:Muramidase / Q000032:analysis*; D010587:Phagocytosis
chemical_list: D007840:Latex; D009113:Muramidase