Simple method to purify chicken immunoglobulin G.
A new method for purifying IgG was adapted for use with chicken serum. Chicken serum was mixed with caprylic acid, precipitated with ammonium sulfate, and dialyzed against PBS. Analysis by SDS-PAGE and Western blot confirmed successful purification of chicken IgG. Large quantities of highly purified IgG were easily obtainable for use in immunological investigations or for labeling. This technique is rapid, inexpensive, and simpler to perform than traditional ion exchange or gel filtration chromatography.
Bhanushali
J K
JK
Department of Poultry Science, University of Georgia, Athens 30602.
Gilbert
J M
JM
McDougald
L R
LR
D000645:Ammonium Sulfate; D000818:Animals; D015153:Blotting, Western; D002210:Caprylates; D011232:Chemical Precipitation; D002645:Chickens / Q000276:immunology*; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D051379:Mice
D002210:Caprylates; D007074:Immunoglobulin G; C031492:octanoic acid; D000645:Ammonium Sulfate