title: Construction and characterization of attenuated delta aroA delta virG Shigella flexneri 2a strain CVD 1203, a prototype live oral vaccine.
abstract: We engineered an oral Shigella vaccine prototype that can invade intestinal epithelial cells but cannot undergo extensive intracellular replication or extend to adjacent epithelial cells. Strain CVD 1203, derived from wild-type Shigella flexneri 2a by introducing deletions in chromosomal aroA and invasion plasmid virG, was highly attenuated in the Sereny test. Two 10(9)-CFU orogastric doses (2 weeks apart) stimulated production of secretory immunoglobulin A antibodies to S. flexneri 2a and protected against conjunctival sac challenge with virulent S. flexneri 2a.
authors.affiliation: Department of Pediatrics, University of Maryland School of Medicine, Baltimore 21201.
authors.forename: F R
authors.initials: FR
authors.lastname: Noriega
authors.forename: J Y
authors.initials: JY
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Losonsky
authors.forename: D R
authors.initials: DR
authors.lastname: Maneval
authors.forename: D M
authors.initials: DM
authors.lastname: Hone
authors.forename: M M
authors.initials: MM
authors.lastname: Levine
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D001483:Base Sequence; D017931:DNA Primers; D017353:Gene Deletion; D005798:Genes, Bacterial; D006168:Guinea Pigs; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007637:Keratoconjunctivitis / Q000517:prevention & control; D008969:Molecular Sequence Data; D010957:Plasmids; D012763:Shigella flexneri / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D001428:Bacterial Vaccines; D017931:DNA Primers; D007071:Immunoglobulin A, Secretory; D014613:Vaccines, Attenuated