title: Cochlear blood flow measured by averaged laser Doppler flowmetry (ALDF).
abstract: This report describes a new approach to estimate the hydromechanical properties of a vascular system. Averaged laser Doppler flowmetry (ALDF) was developed by averaging the flux signal of a laser Doppler flowmeter (LDF) synchronized to the heart cycle. The usefulness of this method was verified by manipulation of the cochlear microvasculature. Twelve pigmented guinea pigs under pentobarbital/fentanyl anesthesia were used. The cochlea was surgically exposed and the LDF probe placed on the bony surface of the first turn to monitor cochlear blood flow (CBF). The LDF flux signal (0.2 s time constant) was sampled by an A/D board at 2 kHz for 255 ms and averaged with synchronization to the heart beat. The mean blood flow, peak to peak amplitude, and time (phase) delay of pulsatile flow were measured from the averaged signal. According to a transmission line model of the vascular system, under a given perfusion pressure, mean flow reflects resistance while amplitude and time delay of the pulsatile flow are related to the reactance component of the impedance of the vascular system. During the formation of photochemically-induced thrombosis in the cochlear microvasculature, there was a dramatic mean flux decrease (90.1 +/- 3.4% from baseline (BL), N = 6). Additionally, a time-dependent decrease in amplitude and time delay of pulsatile flow were indicated by ALDF. These results suggest a large increase in vascular resistance and significant decrease in compliance.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Kresge Hearing Research Institute, University of Michigan Medical School, Ann Arbor 48109-0506.
authors.forename: T
authors.initials: T
authors.lastname: Ren
authors.forename: P B
authors.initials: PB
authors.lastname: Brechtelsbauer
authors.forename: J M
authors.initials: JM
authors.lastname: Miller
authors.forename: A L
authors.initials: AL
authors.lastname: Nuttall
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D003051:Cochlea / Q000098:blood supply* / Q000187:drug effects; D015834:Cochlear Diseases / Q000209:etiology / Q000503:physiopathology; D006168:Guinea Pigs; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008833:Microcirculation / Q000187:drug effects / Q000502:physiology; D009599:Nitroprusside / Q000008:administration & dosage; D012405:Round Window, Ear / Q000187:drug effects; D013927:Thrombosis / Q000209:etiology / Q000503:physiopathology; D014655:Vascular Resistance / Q000502:physiology
chemical_list: D009599:Nitroprusside