Transmammary modulation of xenobiotic metabolizing enzymes in liver of mouse pups by mace (Myristica fragrans Houtt.).
The present study examines the possible transfer of the active principle(s) of mace (aril of the plant Myristica fragrans) through the transmammary route and its ability to modulate hepatic xenobiotic metabolizing enzymes in the F1 progeny of mice. An aqueous suspension of mace at the dose levels of 0.025 or 0.1 g/animal/day was administered by oral gavage to dams from day 1 of lactation and continued daily for 14 or 21 days. Dams receiving mace treatment and their F1 pups showed significantly elevated hepatic sulfhydryl content, glutathione S-transferase and glutathione reductase activities and cytochrome b5 content. Hepatic cytochrome P450 content decreased in dams (P < 0.05) receiving the lower mace dose for 21 days and the F1 pups (P < 0.001), but increased in dams receiving the higher dose for both time periods (P < 0.001) and the lower dose for 14 days (P < 0.05). Only the 14-day-old pups of dams receiving either mace dose showed significantly elevated (P < 0.001) levels of hepatic glutathione peroxidase.
Chhabra
S K
SK
Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Rao
A R
AR
D000818:Animals; D016588:Anticarcinogenic Agents / Q000493:pharmacokinetics* / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000378:metabolism; D015786:Cytochromes b5 / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D005980:Glutathione Reductase / Q000187:drug effects / Q000378:metabolism; D005982:Glutathione Transferase / Q000187:drug effects / Q000378:metabolism; D007774:Lactation; D008099:Liver / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D010936:Plant Extracts / Q000493:pharmacokinetics* / Q000494:pharmacology*; D010946:Plants, Medicinal; D011247:Pregnancy; D013438:Sulfhydryl Compounds / Q000378:metabolism; D002721:omega-Chloroacetophenone / Q000494:pharmacology*
D016588:Anticarcinogenic Agents; D010936:Plant Extracts; D013438:Sulfhydryl Compounds; D002721:omega-Chloroacetophenone; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase