Serotonin depletion affects blood-feeding but not host-seeking ability in Aedes triseriatus (Diptera: Culicidae).
The host-seeking and blood-feeding abilities of Aedes triseriatus (Say) were evaluated after treatment with the amine depleting drugs alpha-methyl-tyrosine (AMT) and alpha-methyl-tryptophan (AMTP). High-performance liquid chromatography with electrochemical detection (HPLCED) was used to demonstrate that AMTP depleted serotonin levels, whereas AMT reduced dopamine levels. The host-seeking ability (evaluated with an olfactometer) of Ae. triseriatus was not altered by treatment with either AMT or AMTP. Blood-feeding success was reduced significantly by either oral administration or injection of AMTP. Mosquitoes treated with AMTP responded positively when placed in close proximity to a host (rabbit), but fewer treated than untreated mosquitoes fed to repletion or fed at all. Those feeding to repletion took longer to do so. No differences in feeding ability were observed between control and AMT-treated mosquitoes. These observations indicate that serotonin plays a role in modulating blood-feeding activities of Ae. triseriatus.
Novak
M G
MG
Department of Entomology, Iowa State University, Ames 50011.
Rowley
W A
WA
D000330:Aedes / Q000502:physiology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D001769:Blood; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology; D005260:Female; D008781:Methyltyrosines / Q000494:pharmacology; D011817:Rabbits; D012701:Serotonin / Q000502:physiology*; D014364:Tryptophan / Q000031:analogs & derivatives / Q000494:pharmacology; D019805:alpha-Methyltyrosine
D008781:Methyltyrosines; C020774:alpha-methyltryptophan; D012701:Serotonin; D019805:alpha-Methyltyrosine; D014364:Tryptophan