Detection of IL-6 and its receptor mRNAs in a megakaryocytic cell line, CMK, by an RT-nested PCR.
An autocrine loop in a megakaryocytic cell line, CMK, has been proposed by Fuse et al. They detected IL-6 mRNA by means of Northern blotting, but not IL-6 receptor (IL-6R) mRNA. I also failed to detect IL-6R mRNA by the same means. In this study I showed the expression of IL-6 and its receptor mRNAs by means of reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) with an automated electrophoresis PhastSystem. These two procedures considerably enhanced detection sensitivity. The proliferation and the differentiation of CMK cells are augmented by IL-6 and inhibited by anti-IL-6 antibody. This study continued the autocrine theory of Fuse et al. in CMK cells. Moreover, this is the first detection of IL-6R mRNA in CMK.
Nakayama
K
K
Department of Laboratory Medicine, Kobe University School of Medicine, Japan.
D002460:Cell Line; D004587:Electrophoresis, Agar Gel; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008533:Megakaryocytes / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis*; D018123:Receptors, Interleukin / Q000235:genetics*; D019947:Receptors, Interleukin-6
D015850:Interleukin-6; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6