The role of peptide leukotrienes in renal failure induced by a tourniquet.
To examine the role of peptide leukotrienes in tourniquet-induced renal failure, hindlimbs of rabbits were fastened for 5 h and then released for 6 h. Blood urea nitrogen (BUN) increased significantly at the 3-h release. In the fastened muscle, peptide leukotrienes LTC4, LTD4 and LTE4 increased immediately after or at the 3-h release. LTD4 and LTE4 in the blood increased at the 6-h release. The edematous destruction of renal microvessels and proximal tubule cells was observed at the 3-h release. Glomerular changes were not observed. Immunoreactions of LTC4/D4 were localized in the lysosomes of the macrophages, leukocytes and fibroblasts of the fastened muscles immediately after the release, as well as in the lysosomes of renal proximal tubular cells and on the endothelial cell surface of the renal capillary at the 3-h release. These changes continued up to the end of the release. The lipoxygenase inhibitor attenuated the BUN increase and edema manifestation in the fastened muscle. These results suggest that peptide leukotrienes derived from the fastened skeletal muscle are related to tourniquet-induced renal failure.
Tanaka
T
T
Department of Forensic Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Kita
T
T
Tanaka
N
N
D058186:Acute Kidney Injury / Q000209:etiology* / Q000378:metabolism; D000818:Animals; D001806:Blood Urea Nitrogen; D002196:Capillaries / Q000473:pathology; D003402:Creatine Kinase / Q000097:blood; D004487:Edema / Q000378:metabolism; D004730:Endothelium, Vascular / Q000648:ultrastructure; D006614:Hindlimb / Q000098:blood supply*; D007150:Immunohistochemistry; D007668:Kidney / Q000098:blood supply / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000378:metabolism*; D011817:Rabbits; D015427:Reperfusion Injury / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D013189:SRS-A / Q000378:metabolism*; D014111:Tourniquets / Q000009:adverse effects*
D013189:SRS-A; D003402:Creatine Kinase