Apoptosis induced in mouse hepatitis virus-infected cells by a virus-specific CD8+ cytotoxic T-lymphocyte clone.
Morphological changes of mouse hepatitis virus-infected J774.1 cells cocultured with cloned mouse hepatitis virus-specific CD8+ cytotoxic T lymphocytes were examined by electron microscopy. Condensation and margination of chromatin, cellular shrinkage with severe vacuolar degeneration, and blebbing were observed. In addition, fragmentation of cellular DNA was observed, and a decrease in virus titer was accompanied by those changes. These findings show that the cloned cytotoxic T lymphocytes induce in the target cell an internal degradation program termed apoptosis, which results in virus clearance.
Shibata
S
S
Laboratory of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, Japan.
Kyuwa
S
S
Lee
S K
SK
Toyoda
Y
Y
Goto
N
N
D000818:Animals; D017209:Apoptosis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D002448:Cell Adhesion; D002478:Cells, Cultured; D002999:Clone Cells; D004247:DNA / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D006517:Murine hepatitis virus / Q000276:immunology* / Q000472:pathogenicity; D014779:Virus Replication
D004247:DNA