Effect of tetracyclines on collagenase activity in patients with recurrent aphthous ulcers.
Human neutrophil-type (MMP-8) and fibroblast-type (MMP-1) interstitial collagenase, and their inhibition by tetracyclines in saliva from patients with recurrent aphthous ulcers (RAU) or aphthae, were studied by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and enzymological analyses. In the salivary specimens obtained from patients with aphthae, collagenase was found in endogenously active form and was predominantly of MMP-8 type. Topical rinsing treatment with chlortetracycline (Aureomycin) alleviated the discomfort caused by the lesions but did not reduce salivary collagenase amounts; however in vitro, doxycycline inhibited salivary collagenase totally.
Häyrinen-Immonen
R
R
Department of Oral and Maxillofacial Surgery, University of Helsinki, Finland.
Sorsa
T
T
Pettilä
J
J
Konttinen
Y T
YT
Teronen
O
O
Malmström
M
M
D000293:Adolescent; D000328:Adult; D002648:Child; D002751:Chlortetracycline / Q000008:administration & dosage / Q000627:therapeutic use*; D017364:Collagenases / Q000378:metabolism; D004318:Doxycycline / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1; D020784:Matrix Metalloproteinase 8; D061965:Matrix Metalloproteinase Inhibitors; D008875:Middle Aged; D009067:Mouthwashes / Q000627:therapeutic use; D009504:Neutrophils / Q000201:enzymology; D012008:Recurrence; D012463:Saliva / Q000201:enzymology; D013281:Stomatitis, Aphthous / Q000188:drug therapy* / Q000201:enzymology*
D061965:Matrix Metalloproteinase Inhibitors; D009067:Mouthwashes; D017364:Collagenases; D020784:Matrix Metalloproteinase 8; D020781:Matrix Metalloproteinase 1; D004318:Doxycycline; D002751:Chlortetracycline