title: Coat protein sequences of RMV-like strains of barley yellow dwarf virus separate them from other luteoviruses.
abstract: Illinois (IL) and Minnesota (MN) RMV-like strains of barley yellow dwarf virus (BYDV) were identified from maize displaying red leaf symptoms by enzyme-linked immunosorbent assay (ELISA) using antiserum against a New York strain (BYDV-RMV-NY). Some IL and MN strains, but not the NY strain, could be detected by ELISA with a monoclonal antibody raised against BYDV-RPV-NY. The region of the viral genome representing the coat protein gene was amplified by polymerase chain reaction, cloned and sequenced. The nucleotide sequences of the BYDV-RMV-IL and BYDV-RMV-MN coat protein genes differed at just five nucleotide positions while the BYDV-RMV-IL and BYDV-RMV-NY differed at 101 of the 591 positions. The predicted amino acid sequences of the coat proteins of RMV-like strains from IL, MN, and NY shared approximately 60% identify with those of the coat proteins of beet western yellows virus, BYDV-RPV-NY, and potato leafroll virus.
authors.affiliation: Department of Plant Pathology, University of Illinois, Urbana 61801.
authors.forename: L L
authors.initials: LL
authors.lastname: Domier
authors.forename: L I
authors.initials: LI
authors.lastname: Lukasheva
authors.forename: C J
authors.initials: CJ
authors.lastname: D'Arcy
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D017793:Luteovirus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015678:Viral Structural Proteins / Q000235:genetics*; D003313:Zea mays / Q000821:virology
chemical_list: D015678:Viral Structural Proteins