title: Lectin electron microscopic histochemistry of the pseudoexfoliative material in the skin.
abstract: OBJECTIVE
  To investigate the hypothesis that pseudoexfoliation (PSX) syndrome is a systemic disorder, the authors studied the composition of glycoconjugates in the intraocular and extraocular PSX material at the electron microscopic level, using a panel of lectins as cytochemical probes.
  
  
  METHODS
  The authors examined 8 lid skins, 11 trabecular tissues, and 3 cataractous lenses from human eyes with PSX syndrome. Tissues were processed for electron microscopical histochemistry and stained with PNA, RCA120, DBA, SBA, ConA, WGA, UEA-I, and Lotus, with an indirect lectin-colloidal gold technique.
  
  
  RESULTS
  Both the intraocular and extraocular PSX materials manifested almost identical reactivity to lectins, which indicated that glycoconjugates in the PSX material contained with sugar residues of galactose (PNA, RCA120), alpha-mannose (ConA), and N-acetyl-D-glucosamine (WGA). On the other hand, it was indicated that sugar residues of N-acetyl-D-galactosamine (DBA, SBA) and fucose (UEA-I, Lotus) were absent. Granular inclusions and microfibrils in the capsule and ocular zonules were stained similarly and weakly.
  
  
  CONCLUSIONS
  The intraocular and extraocular PSX materials contained the same sugar residues of glycoconjugates, which suggested that those materials had the same nature. This study, the first documentation of lectin-binding sites on the extraocular PSX material, supported the hypothesis of PSX syndrome as a systemic disorder.
authors.affiliation: Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Amari
authors.forename: S
authors.initials: S
authors.lastname: Nagata
authors.forename: J
authors.initials: J
authors.lastname: Umihira
authors.forename: M
authors.initials: M
authors.lastname: Nohara
authors.forename: N
authors.initials: N
authors.lastname: Usuda
authors.forename: K
authors.initials: K
authors.lastname: Segawa
mesh_terms: D017889:Exfoliation Syndrome / Q000378:metabolism* / Q000473:pathology; D005143:Eyelids / Q000378:metabolism / Q000648:ultrastructure; D006001:Glycoconjugates / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D009844:Oligosaccharides / Q000032:analysis; D012867:Skin / Q000378:metabolism* / Q000648:ultrastructure; D014129:Trabecular Meshwork / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D009844:Oligosaccharides