title: Induction of high-affinity GM-CSF receptors during all-trans retinoic acid treatment of acute promyelocytic leukemia.
abstract: Differentiation of normal myeloid cells is accompanied by the increase of high-affinity GM-CSF receptors necessary for progenitor proliferation/differentiation and mature neutrophil function. All-trans retinoic acid (ATRA) induces terminal differentiation of acute promyelocytic leukemia cells (AML3 subtype). We report in this study that AML3 cells, like other AML subtypes, harbor high-affinity GM-CSF R (n = 138.3 +/- 69.3 sites/cell, Kd = 76.9 +/- 68.8 pM). In all cases, incubation with ATRA induces either an increase in the number of affinity of GM-CSF R (n = 212.7 +/- 116.2 sites/cell, Kd = 43.2 +/- 22.5 pM). The data presented show that modulation of GM-CSF receptors cells is correlated to the degree of ATRA-induced granulocytic differentiation but not to increased cell growth.
authors.affiliation: Laboratoire de Biologie Cellulaire Hématopoiétique, Hôpital Saint-Louis, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: de Gentile
authors.forename: M E
authors.initials: ME
authors.lastname: Toubert
authors.forename: C
authors.initials: C
authors.lastname: Dubois
authors.forename: I
authors.initials: I
authors.lastname: Krawice
authors.forename: M H
authors.initials: MH
authors.lastname: Schlageter
authors.forename: N
authors.initials: N
authors.lastname: Balitrand
authors.forename: S
authors.initials: S
authors.lastname: Castaigne
authors.forename: L
authors.initials: L
authors.lastname: Degos
authors.forename: J D
authors.initials: JD
authors.lastname: Rain
authors.forename: Y
authors.initials: Y
authors.lastname: Najean
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism* / Q000473:pathology; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D014212:Tretinoin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor