title: Kin17, a mouse nuclear zinc finger protein that binds preferentially to curved DNA.
abstract: Kin17 is a 45 kDa protein encoded by the KIN17 gene located on mouse chromosome 2, band A. The kin17 amino acid sequence predicts two domains, which were shown to be functional: (i) a bipartite nuclear localization signal (NLS) that can drive the protein to the cell nucleus, (ii) a bona fide zinc finger of the C2H2 type. The zinc finger is involved in kin17 binding to double-stranded DNA since a mutant deleted of the zinc finger, kin17 delta 1, showed reduced binding. Single-stranded DNA was bound poorly by kin17. Interestingly, we found that kin17 protein showed preferential binding to curved DNA from either pBR322 or synthetic oligonucleotides. Binding of kin17 to a non-curved DNA segment increased after we had inserted into it a short curved synthetic oligonucleotide. Kin17 delta 2, a mutant deleted of 110 amino acids at the C-terminal end, still exhibited preferential binding to curved DNA and so did kin17 delta 1, suggesting that a domain recognizing curved DNA is located in the protein core.
authors.affiliation: G.E. Mutagenèse et Cancérogenèse, Institut Curie, Centre Universitaire, Orsay, France.
authors.forename: A
authors.initials: A
authors.lastname: Mazin
authors.forename: T
authors.initials: T
authors.lastname: Timchenko
authors.forename: J
authors.initials: J
authors.lastname: Ménissier-de Murcia
authors.forename: V
authors.initials: V
authors.lastname: Schreiber
authors.forename: J F
authors.initials: JF
authors.lastname: Angulo
authors.forename: G
authors.initials: G
authors.lastname: de Murcia
authors.forename: R
authors.initials: R
authors.lastname: Devoret
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D015183:Restriction Mapping; D013329:Structure-Activity Relationship; D016335:Zinc Fingers; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C071214:Kin protein, mouse; D004247:DNA; D001616:beta-Galactosidase