title: Nuclear protein 780BP from cauliflower binds an element in the 780 gene promoter of T-DNA.
abstract: A 16 bp site of protein binding has been identified in the promoter of the 780 gene of T-DNA. Specific DNA-protein interactions were demonstrated between a double-stranded oligonucleotide containing this element (5'-TTGAAAAATCAACGCT-3') and a protein isolated from nuclear extracts of cauliflower inflorescences. Specific bases required for this binding activity (780 binding protein; 780BP) were defined by kinetic competition studies with mutated oligonucleotides, methylation interference assays and DNAse I footprinting. 780BP binding was not competed with up to 1000-fold excess of previously characterized plant regulatory elements such as as-1, the LRE, and the ocs, G-box, and AT-rich elements. In addition, 780BP was shown to bind sequences overlapping a mammalian hormone receptor element with greater affinity than the 780 element.
authors.affiliation: Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
authors.forename: E C
authors.initials: EC
authors.lastname: Adams
authors.forename: W B
authors.initials: WB
authors.lastname: Gurley
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D001937:Brassica / Q000378:metabolism*; D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008745:Methylation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011131:Polyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D002457:Cell Extracts; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D011131:Polyribonucleotides; C036483:T-DNA