Identification of genes involved in utilization of acetate and acetoin in Bacillus subtilis.
The Bacillus subtilis ccpA gene has previously been shown to be involved in repression of amyE expression when cells are grown in excess glucose. The region of the B. subtilis chromosome downstream from ccpA was characterized to determine if additional genes involved in carbohydrate metabolism were present. Two open reading frames that exhibited sequence similarity to the Escherichia coli and B. subtilis motA and motB motility genes were found immediately downstream from ccpA; disruption of this region had no effect on growth, sporulation or motility. Two divergent transcriptional units containing the acsA and acuABC genes were also found in this region. The acsA gene encodes acetyl-CoA synthetase, and inactivation of this gene resulted in loss of the ability to utilize acetate as a carbon source for growth or sporulation. Disruption of the acuABC genes resulted in poor growth or sporulation on acetoin or butanediol. The acsA and acuABC promoter sequences were identified by primer extension, and are in close proximity. Two sequences resembling the amyO regulatory target site necessary for glucose repression of amyE were identified in the acsA-acuABC promoter regions.
Grundy
F J
FJ
Department of Biochemistry and Molecular Biology, Albany Medical College, New York 12208.
Waters
D A
DA
Takova
T Y
TY
Henkin
T M
TM
D000106:Acetate-CoA Ligase / Q000235:genetics; D000085:Acetates / Q000378:metabolism*; D000093:Acetoin / Q000378:metabolism*; D000123:Acetyltransferases; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012097:Repressor Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013171:Spores, Bacterial / Q000254:growth & development; D014158:Transcription, Genetic; D014448:Tyrosine-tRNA Ligase / Q000235:genetics
D000085:Acetates; C089829:AcuC protein, Bacillus subtilis; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C089828:acuB protein, Bacillus subtilis; C069286:catabolite control proteins, bacteria; D000093:Acetoin; D000123:Acetyltransferases; C089827:acuA protein, Bacillus subtilis; D014448:Tyrosine-tRNA Ligase; D000106:Acetate-CoA Ligase