Infection with human cytomegalovirus enhances bacterial adhesiveness and invasiveness in permissive and semipermissive cells.
The effect of human cytomegalovirus (HCMV) infection on adhesiveness and invasiveness of Salmonella typhimurium was examined in cells permissive (human embryo fibroblasts (HE)), semipermissive (A549) and nonpermissive (HEp-2) for the virus. Preinfection of the cells with HCMV induced enhanced adhesiveness and invasiveness of bacteria in the permissive HE cells. In the semipermissive A549 cells, where HCMV immediate-early (IE) mRNA transcripts and IE proteins were detected, a significant effect on the initial phase of invasiveness, the adherence phase, was demonstrated. HCMV had no effect on invasiveness of S. typhimurium in nonpermissive HEp-2 cells. Neither HCMV IE transcripts nor IE proteins could be detected in these cells.
Holberg-Petersen
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M
Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Norway.
Bukholm
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G
Rollag
H
H
Degré
M
M
D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics; D001422:Bacterial Adhesion; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D003588:Cytopathogenic Effect, Viral; D017931:DNA Primers / Q000737:chemistry; D005347:Fibroblasts / Q000382:microbiology* / Q000821:virology; D005455:Fluorescent Antibody Technique; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics; D007124:Immunoenzyme Techniques; D007822:Laryngeal Neoplasms; D008175:Lung Neoplasms; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012486:Salmonella typhimurium / Q000472:pathogenicity*; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D000956:Antigens, Viral; D017931:DNA Primers; D017874:Immediate-Early Proteins; D012333:RNA, Messenger; C044582:immediate-early proteins, cytomegalovirus