title: A donor-derived asialo-GM1+ cell induces depression of B-cell genesis during systemic graft-versus-host disease.
abstract: The nature of the effector cell(s) responsible for the depression of B-cell genesis in the bone marrow of mice undergoing systemic graft-versus-host disease (GVHD) has been examined. Donor C57BL/6 (B6) mice were treated in vivo with either a single injection of anti-asialo GM1 antibody (anti-ASGM1) to eliminate naturally occurring (endogenous) ASGM1+ cells or B6xAF1 (B6AF1) lymphoid cells followed by anti-ASGM1 to eliminate both endogenous and "induced" ASGM1+ cells. Lymphoid cells from donor mice after the elimination of endogenous ASGM1+ cells produced severe GVHD and concomitant depression of B-cell genesis when injected into B6AF1 recipients. In contrast, cells from donors depleted of both the endogenous and inducible ASGM1+ populations did not cause GVHD or depletion of B lineage cells in B6AF1 recipients but did depress B-cell genesis in B6C3F1 mice. The "induced" ASGM1+ cells were Thy 1+, but their elimination did not significantly alter either overall T-cell function or specific cytotoxic T-cell (CTL) reactivity against the sensitizing (B6AF1) strain. The results suggest that the effector cell responsible for the depression of B-cell genesis during systemic GVHD can be induced to express ASGM1, is strain-specific and Thy 1+; but is not a conventional CTL.
authors.affiliation: Department of Physiology, McGill University, Montreal, Quebec, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Xenocostas
authors.forename: T
authors.initials: T
authors.lastname: Ghayur
authors.forename: J C
authors.initials: JC
authors.lastname: Setrakian
authors.forename: W S
authors.initials: WS
authors.lastname: Lapp
authors.forename: D G
authors.initials: DG
authors.lastname: Osmond
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use; D001402:B-Lymphocytes; D016026:Bone Marrow Transplantation / Q000473:pathology*; D005260:Female; D005677:G(M1) Ganglioside / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000276:immunology; D006086:Graft vs Host Disease / Q000473:pathology*; D006410:Hematopoiesis; D007694:Killer Cells, Natural / Q000276:immunology* / Q000473:pathology / Q000637:transplantation; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013154:Spleen / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside