pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos.
We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1 alpha-encoding gene (EF-1 alpha). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.
Johnson
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Department of Zoology, University of Texas at Austin 78712.
Krieg
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D000818:Animals; D001755:Blastocyst; D003001:Cloning, Molecular; D046508:Culture Techniques; D004625:Embryo, Nonmammalian; D005822:Genetic Vectors; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic; D014982:Xenopus laevis
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012333:RNA, Messenger