HPLC assay for atropine in serum and protein solutions after in vitro addition of the tropane alkaloid.
A simple and sensitive method for the determination of atropine in serum and protein solutions is presented. Atropine and procaine (internal standard) were extracted with diethylether from protein containing solutions after alkalinization. The residue of the evaporized ether phase, redissolved in diluted sulfuric acid, was injected directly onto a LiChrosorbR column. Atropine, procaine and serum constituents were separated by HPLC using an ion-pair containing mobile phase. The limit for the detection of atropine was 200 ng/ml. The method was used for the determination of atropine in a binding study. Percent binding values to human serum, human serum albumin and human alpha 1-acid glycoprotein were evaluated by equilibrium dialysis.
Büch
U
U
Klinik für Anästhesie und Intensivmedizin, Universität des Saarlandes, Homburg/Saar, Germany.
Isenberg
E
E
Büch
H P
HP
D001285:Atropine / Q000032:analysis* / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis* / Q000378:metabolism; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009961:Orosomucoid / Q000378:metabolism; D011343:Procaine / Q000032:analysis; D011485:Protein Binding; D012015:Reference Standards; D012709:Serum Albumin / Q000378:metabolism; D012996:Solutions
D001798:Blood Proteins; D009961:Orosomucoid; D012709:Serum Albumin; D012996:Solutions; D011343:Procaine; D001285:Atropine