Relative glucocorticoid potency revisited.
To determine the relative potency of synthetic glucocorticoids, glucocorticoid receptor expressing cells were transfected with a hormone-inducible reporter gene, and were cultured in the presence of various glucocorticoid ligands. Hormonal inducibility was determined by means of a chloramphenicol acetyltransferase assay. Dexamethasone and prednisolone, as well as cortisol, induced the expression of the reporter gene in a dose-dependent fashion. The relative potency of each ligand was in this order when inducibility was quantitatively assessed. In conclusion, the transcription assay described here may be a convenient and alternative method to evaluate the relative potency of given glucocorticoids.
Tanaka
H
H
Second Department of Internal Medicine, Asahikawa Medical College, Japan.
Hirano
F
F
Nomura
Y
Y
Miura
T
T
Makino
Y
Y
Fukawa
E
E
Makino
I
I
D000818:Animals; D001681:Biological Assay; D016466:CHO Cells; D002461:Cell Line, Transformed; D015500:Chloramphenicol O-Acetyltransferase; D006224:Cricetinae; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic; D018014:Gene Transfer Techniques; D005938:Glucocorticoids / Q000235:genetics / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D008324:Mammary Tumor Virus, Mouse; D011239:Prednisolone / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011965:Receptors, Glucocorticoid / Q000187:drug effects* / Q000235:genetics; D014158:Transcription, Genetic
D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D011239:Prednisolone; D015500:Chloramphenicol O-Acetyltransferase; D006854:Hydrocortisone