title: Cloning, sequence and in vitro transcription/translation analysis of a 3.2-kb EcoRI-HindIII fragment of Leuconostoc oenos bacteriophage L10.
abstract: A 3.2-kb EcoRI-HindIII DNA fragment of Leuconostoc oenos bacteriophage L10 was cloned and sequenced. Computer-assisted analysis of the sequence identified eleven possible open reading frames (ORFs) that were all on the same strand. In vitro transcription/translation analysis of the full-length DNA fragment yielded five prominent proteins that were correlated with ORFs by their sizes and expression from deleted clones. Only those ORFs containing recognizable Shine-Dalgarno sequences coded for proteins. Neither the nucleotide sequence, nor deduced amino-acid sequences showed significant homology with other known sequences.
authors.affiliation: Department of Microbiology, University of Stellenbosch, South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Sutherland
authors.forename: H J
authors.initials: HJ
authors.lastname: van Vuuren
authors.forename: M M
authors.initials: MM
authors.lastname: Howe
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D015870:Gene Expression; D007934:Leuconostoc / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D014176:Protein Biosynthesis; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000032:analysis
chemical_list: D003062:Codon; D014764:Viral Proteins