In vivo production of heat shock protein in mouse peritoneal macrophages by administration of lipopolysaccharide.
The in vivo production of heat shock protein was studied by administration of bacterial lipopolysaccharide (LPS) into mice. Heat shock protein 70 was detected in the extract of adherent peritoneal cells from mice injected intraperitoneally with LPS by using the immunoblotting method. The expression of heat shock protein 70 was found 2 days after injection of LPS and reached its peak 4 days after injection. The intraperitoneal injection of LPS induced the expression of heat shock protein 70, whereas its subcutaneous injection did not. The in vivo production of heat shock protein 70 was inhibited by administration of LPS together with quercetin, an inhibitor of accumulation of heat shock protein 70 mRNA. Tumor necrosis factor alpha enhanced LPS-induced heat shock protein production in vivo. There was a decrease of gamma delta T cells in the peritoneal cavity of mice injected intraperitoneally with LPS. It was suggested that bacterial LPS is a stressful agent which induces the in vivo heat shock protein response, and its administration leads to the production of heat shock protein 70 in peritoneal macrophages.
Zhang
Y H
YH
Department of Microbiology, Aichi Medical University, Japan.
Takahashi
K
K
Jiang
G Z
GZ
Zhang
X M
XM
Kawai
M
M
Fukada
M
M
Yokochi
T
T
D000818:Animals; D016207:Cytokines / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D011794:Quercetin / Q000494:pharmacology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D013601:T-Lymphocytes / Q000187:drug effects
D016207:Cytokines; D006360:Heat-Shock Proteins; D008070:Lipopolysaccharides; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011794:Quercetin