title: Involvement of Fc epsilon RII/CD23 and L-arginine-dependent pathway in IgE-mediated stimulation of human monocyte functions.
abstract: Elevated IgE levels are commonly observed during the inflammatory responses in allergy and a variety of infections. This Ig activates the release of multiple mediators from monocytes/macrophages. In the present work, we attempted to clarify the IgE-dependent events involved in the activation of monocyte functions. IgE-anti-IgE immune complexes induce the production of tumor necrosis factor-alpha, oxygen radicals, IL-6 and thromboxane B2 from normal human purified monocytes. Expression and cross-linkage of Fc epsilon RII/CD23 were essential for these IgE-mediated effects. Cytokine production following CD23 ligation depended on nitric oxide transduction pathway, as it was inhibited by NG-monomethyl-L-arginine, a competitive inhibitor of the conversion of L-arginine to L-citroline by nitric oxide synthase. Furthermore, addition of the nitric oxide chemical donator, Sin-1, enhanced IgE-induced monokine release. CD23-ligation also induced the production of nitrites by these cells. This work linked CD23 to the L-arginine-dependent transduction pathway and shows their involvement in IgE-mediated stimulation of human monocytes.
authors.affiliation: Groupe d'Immuno-Hématologie Moléculaire, CNRS URA625, Hôpital Pitié-Salpêtrière, Paris, France.
authors.forename: M D
authors.initials: MD
authors.lastname: Mossalayi
authors.forename: N
authors.initials: N
authors.lastname: Paul-Eugène
authors.forename: F
authors.initials: F
authors.lastname: Ouaaz
authors.forename: M
authors.initials: M
authors.lastname: Arock
authors.forename: J P
authors.initials: JP
authors.lastname: Kolb
authors.forename: E
authors.initials: E
authors.lastname: Kilchherr
authors.forename: P
authors.initials: P
authors.lastname: Debré
authors.forename: B
authors.initials: B
authors.lastname: Dugas
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives / Q000276:immunology* / Q000494:pharmacology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000502:physiology; D017455:Receptors, IgE / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D013481:Superoxides / Q000378:metabolism; D013929:Thromboxane B2 / Q000096:biosynthesis; D019323:omega-N-Methylarginine
chemical_list: D016207:Cytokines; D017455:Receptors, IgE; D013481:Superoxides; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D007073:Immunoglobulin E; D013929:Thromboxane B2; C002385:linsidomine; D001120:Arginine; D008981:Molsidomine