Complete nucleotide sequence and tissue-specific expression of the rat phenylethanolamine N-methyltransferase gene.
The rat phenylethanolamine N-methyltransferase (PNMT) gene was isolated from a genomic library by cross-hybridization with a bovine PNMT cDNA probe. Complete nucleotide sequence analysis of a genomic clone showed that this gene contained three exons and spanned about 2.8 kb in length. There were the acute-phase response element, TATA, SP1, and GRE sequences. The physicochemical properties of rat adrenal PNMT were different from those of the brainstem PNMT. However, northern blot and reverse transcription-polymerase chain reaction analysis showed that the rat PNMT gene may not express the multiple forms of mRNA. These results suggest that the rat PNMT gene might produce a single enzyme protein, whose activity may be differentially modulated by tissue-specific environment in the central and peripheral systems.
Suh
Y H
YH
Department of Pharmacology, College of Medicine, Seoul National University, South Korea.
Chun
Y S
YS
Lee
I S
IS
Kim
S S
SS
Choi
W
W
Chong
Y H
YH
Hong
L
L
Kim
S H
SH
Park
C W
CW
Kim
C G
CG
D000311:Adrenal Glands / Q000737:chemistry / Q000201:enzymology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001933:Brain Stem / Q000737:chemistry / Q000201:enzymology; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010625:Phenylethanolamine N-Methyltransferase / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D004247:DNA; D010625:Phenylethanolamine N-Methyltransferase