Accessibility of nucleic acid-complexed biomolecules to hydroxyl radicals correlates with their conformation: a fluorescence polarization spectroscopy study.
A fluorescence methodology has been developed to examine the relationship between the conformational state of specific biomolecules in simple chromatin models and their accessibility to hydroxyl radicals (OH). Polylysine and histone H1 were labelled with SECCA, the succinimidyl ester of coumarin-3-carboxylic acid, which generates the fluorescent derivative 7-OH-SECCA following its interaction with radiation-induced OH in aqueous solution. The fluorescence induced per unit gamma-ray dose reflecting the accessibility of OH to such SECCA-conjugated biomolecules was recorded. The biomolecules were also labelled with the fluorescent derivative 7-OH-SECCA in trace amounts to study their conformation under identical conditions via fluorescence polarization spectroscopy. When these biomolecules were complexed with a polynucleotide or DNA, a major increase in polarization anisotropy was recorded. Upon salt-induced dissociation of these biomolecules from the nucleic acids, the increase in anisotropy was reversed. The histone H1-DNA complexes also exhibited an initial increase in anisotropy with increasing NaCl concentration (maximum at 100 mmol dm-3) indicating the possible formation of a more compact conformation. The fluctuations in anisotropy were inversely proportional to the recorded fluorescence/Gy. The data indicate a direct correlation between the accessibility of OH to polylysine or histone H1 complexed with nucleic acids and the conformation of these biomolecules.
Makrigiorgos
G M
GM
Department of Radiation Oncology, Harvard Medical School, Boston, MA 02115.
Bump
E
E
Huang
C
C
Baranowska-Kortylewicz
J
J
Kassis
A I
AI
D004247:DNA / Q000737:chemistry*; D005454:Fluorescence Polarization; D005720:Gamma Rays; D006657:Histones / Q000737:chemistry*; D017665:Hydroxyl Radical; D008968:Molecular Conformation; D011107:Polylysine / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D006657:Histones; D011107:Polylysine; D017665:Hydroxyl Radical; D004247:DNA