title: Protein composition of the human heart: the construction of a myocardial two-dimensional electrophoresis database.
abstract: Molecular changes occurring in myocardial diseases are not well understood. Proteins, as regulatory molecules, should play an important role in the etiology of these diseases. The method of two-dimensional electrophoresis (2-DE) allows the analysis of some thousand proteins with one experiment. An important prerequisite for this kind of investigation is the possibility of identifying the proteins separated by 2-DE. We resolve 3239 proteins of the human myocardium and tried to identify 33 proteins by amino acid analysis and microsequencing. Twenty proteins were identified by search for the protein-chemical data obtained in the Martinsried Institute Protein Sequence Database. Comparisons of 2-DE patterns of different size, which were obtained in different laboratories, were performed with the result that proteins identified on a 2-DE map of one laboratory can be assigned to spots of 2-DE maps produced by another laboratory. Our results show the usefulness of a myocardial 2-DE database; they can be used in different laboratories and make it possible to generate a collection of important human myocardial proteins in a 2-DE database for comparative studies worldwide.
authors.affiliation: Deutsches Herzzentrum Berlin, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Jungblut
authors.forename: A
authors.initials: A
authors.lastname: Otto
authors.forename: E
authors.initials: E
authors.lastname: Zeindl-Eberhart
authors.forename: K P
authors.initials: KP
authors.lastname: Plessner
authors.forename: M
authors.initials: M
authors.lastname: Knecht
authors.forename: V
authors.initials: V
authors.lastname: Regitz-Zagrosek
authors.forename: E
authors.initials: E
authors.lastname: Fleck
authors.forename: B
authors.initials: B
authors.lastname: Wittmann-Liebold
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D001322:Autoanalysis; D002311:Cardiomyopathy, Dilated / Q000378:metabolism*; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007525:Isoelectric Focusing; D007753:Laboratories; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*
chemical_list: D000596:Amino Acids; D011506:Proteins