Isolation of cDNAs encoding guanine nucleotide-binding protein beta-subunit homologues from maize (ZGB1) and Arabidopsis (AGB1).
We have isolated cDNAs from maize (ZGB1) and Arabidopsis (AGB1) encoding proteins homologous to beta subunits of guanine nucleotide-binding protein (G protein). The predicted ZGB1 and AGB1 gene products are 76% identical to each other and 41% or more identical to animal G protein beta subunits. Both predicted proteins contain seven repeats of the so-called "WD-40" motif, where WD is Trp-Asp. RNA blot analysis indicates that ZGB1 mRNA is present in the root, leaf, and tassel and that AGB1 mRNA is expressed in the root, leaf, and flower. DNA blot hybridizations indicate that maize and Arabidopsis genomes contain no other genes that are highly similar to ZGB1 and AGB1, respectively, suggesting that the newly isolated G protein beta-subunit homologues are likely to have unique functions. Furthermore, these G protein beta-subunit homologues are conserved among other plant species and may play important role(s) in plant signaling.
Weiss
C A
CA
Cold Spring Harbor Laboratory, NY 11724.
Garnaat
C W
CW
Mukai
K
K
Hu
Y
Y
Ma
H
H
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000302:isolation & purification; D044387:GTP-Binding Protein beta Subunits; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant; D020962:Heterotrimeric GTP-Binding Proteins; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000235:genetics / Q000378:metabolism*
C089590:AGB1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D044387:GTP-Binding Protein beta Subunits; D046911:Macromolecular Substances; D010940:Plant Proteins; C089589:ZGB1 protein, Zea mays; D019204:GTP-Binding Proteins; D020962:Heterotrimeric GTP-Binding Proteins