title: Ultrastructural localization of major basic protein in the human eosinophil lineage in vitro.
abstract: We examined the ultrastructural localization of (a) a secondary granule matrix protein--eosinophil peroxidase (EPO)--by cytochemistry, (b) a secondary granule core protein (major basic protein, MBP) by immunogold labeling, and (c) a primary granule protein (the Charcot-Leyden crystal protein, CLC protein) by immunogold labeling in eosinophilic myelocytes (EMs) and mature, activated eosinophils that differentiated from umbilical cord blood progenitors cultured in the presence of recombinant human interleukin-5 (rhIL-5). These studies provide the first substructural localization of MBP to condensing cores of immature secondary granules of EMs, as well as identification of unicompartmental, MBP-rich secondary granules that are devoid of matrix compartments and EPO content and are not primary granules by virtue of their lack of CLC protein. These granules occur in quantity in IL-5-activated mature human eosinophils, which have previously been shown to actively transport EPO from the matrix compartments of their secondary granules to the extracellular milieu in smooth membrane-bound cytoplasmic vesicles, a secretory process termed piecemeal degranulation, whereby eosinophils progressively empty cytoplasmic granules of their contents in the absence of classical granule extrusion.
authors.affiliation: Department of Pathology, Beth Israel Hospital, Boston, MA 02215.
authors.forename: A M
authors.initials: AM
authors.lastname: Dvorak
authors.forename: T
authors.initials: T
authors.lastname: Furitsu
authors.forename: P
authors.initials: P
authors.lastname: Estrella
authors.forename: L
authors.initials: L
authors.lastname: Letourneau
authors.forename: T
authors.initials: T
authors.lastname: Ishizaka
authors.forename: S J
authors.initials: SJ
authors.lastname: Ackerman
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D005312:Fetal Blood / Q000737:chemistry / Q000166:cytology; D006023:Glycoproteins / Q000032:analysis; D006410:Hematopoiesis / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D015848:Interleukin-5 / Q000494:pharmacology; D008245:Lysophospholipase; D008854:Microscopy, Electron; D012260:Ribonucleases
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D006023:Glycoproteins; D015848:Interleukin-5; D012260:Ribonucleases; D008245:Lysophospholipase; C011711:lysolecithin acylhydrolase