title: Construction of a BALB/c congenic mouse, C.C3H-Lpsd, that expresses the Lpsd allele: analysis of chromosome 4 markers surrounding the Lps gene.
abstract: Development of a congenic BALB/c mouse strain that contains a segment of chromosome 4 including the Lpsd allele of the lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ strain is presented. On the basis of LPS-induced spleen cell mitogenesis, macrophage tumor necrosis factor secretion, and tyrosine phosphorylation in vitro and lethality in galactosamine-sensitized mice in vivo, the C.C3H-Lpsd strain provides a model of LPS hyporesponsiveness that is comparable to that of the parental C3H/HeJ strain. Analysis of markers in this region indicates that length of the donor fragment is approximately 5.5 centimorgans. Thus, the C.C3H-Lpsd strain provides an important genetic tool for analysis of markers in this region and for examining functional effects of Lpsd expression on the BALB/c background.
authors.affiliation: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814.
authors.forename: S N
authors.initials: SN
authors.lastname: Vogel
authors.forename: J S
authors.initials: JS
authors.lastname: Wax
authors.forename: P Y
authors.initials: PY
authors.lastname: Perera
authors.forename: C
authors.initials: C
authors.lastname: Padlan
authors.forename: M
authors.initials: M
authors.lastname: Potter
authors.forename: B A
authors.initials: BA
authors.lastname: Mock
mesh_terms: D000483:Alleles; D000818:Animals; D002875:Chromosomes; D005260:Female; D005819:Genetic Markers; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D008070:Lipopolysaccharides