Use of lux genes in applied biochemistry.
Bioluminescence has emerged in the last decade as a major tool for the study of bacterial adaptation and survival. In addition to the advantages of sensitivity and the real-time, non-invasive nature of this reporter, the imaging potential of using low-light and photon-counting video cameras has been particularly influential in establishing its ascendancy-over more traditional reporter systems. This review provides a reflection of personal activity in this field through applications in Food Microbiology and collaboration with colleagues both in the UK and beyond.
Hill
P J
PJ
Department of Applied Biochemistry and Food Science, University of Nottingham, Sutton Bonington, Leicestershire, UK.
Stewart
G S
GS
D001419:Bacteria / Q000235:genetics; D018407:Bacterial Physiological Phenomena; D004926:Escherichia coli / Q000235:genetics*; D005516:Food Microbiology; D015870:Gene Expression; D005819:Genetic Markers; D005821:Genetic Techniques; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics*; D008163:Luminescent Measurements; D015398:Signal Transduction; D014733:Vibrio / Q000201:enzymology
D005819:Genetic Markers; D008156:Luciferases