title: Chromatographic analysis of selected tetracyclines from dosage forms and bulk drug substance using polymeric columns with acidic mobile phases.
abstract: The LC analysis of selected tetracyclines from dosage forms and bulk drug substance using polymeric columns has been studied. Mobile phases containing acetonitrile-0.02 M sodium perchlorate (pH 2.0) were used. The tetracyclines were detected by their absorbance at 280 nm. The columns included: a polystyrene-divinylbenzene (PS-DVB) column and a polymethacrylate column with octadecyl ligands (PM-C18). Performance of the two columns was compared and applicability of the described methods for compendial use has been evaluated. The tetracyclines investigated include: minocycline, oxytetracycline, tetracycline, demeclocycline, chlortetracycline, methacycline, doxycycline and meclocycline.
authors.affiliation: Department of Medicinal Chemistry, College of Pharmacy, University of Georgia, Athens 30602-2352.
authors.forename: P D
authors.initials: PD
authors.lastname: Bryan
authors.forename: J T
authors.initials: JT
authors.lastname: Stewart
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004304:Dosage Forms; D006863:Hydrogen-Ion Concentration; D010472:Perchlorates / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D012015:Reference Standards; D012117:Resins, Synthetic; D017670:Sodium Compounds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013754:Tetracyclines / Q000032:analysis*
chemical_list: D000097:Acetonitriles; D004304:Dosage Forms; D010472:Perchlorates; D011109:Polymethacrylic Acids; D011137:Polystyrenes; D012117:Resins, Synthetic; D017670:Sodium Compounds; D013754:Tetracyclines; C030613:polymethacrylic acid; C003771:Amberlite XAD-2 resin; C031068:sodium perchlorate; C032159:acetonitrile