Ethanol-induced signal transducing mechanism associated with a transient antiviral state in human amniotic cells.
Short-term effects of ethanol on human amnion cells were investigated by studying the cellular signaling processes and the replication of vesicular stomatitis virus. Treatment of human amniotic cells with ethanol transiently triggers the breakdown of inositol phospholipids, stimulates intracellular [Ca2+]i mobilization and activates the translocation of protein kinase C. Activation of this signal transduction mechanism is associated with the development of an antiviral state, as proven by studying 3H-uridine incorporation into the RNA of vesicular stomatitis virus. Induction of the antiviral state in human amniotic cells correlates with the solubility of the alcohols in the lipid membrane of the cells.
Markovits
A
A
Microbiological Research Group, Béla Johan National Institute of Hygiene, Budapest, Hungary.
Premecz
G
G
Lörincz
I
I
Bagi
G
G
Nagy
J
J
Farkas
T
T
Földes
I
I
D000650:Amnion / Q000166:cytology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D016899:Interferon-beta / Q000096:biosynthesis*; D010716:Phosphatidylinositols / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D010738:Type C Phospholipases / Q000378:metabolism*; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D014779:Virus Replication / Q000187:drug effects*
D010716:Phosphatidylinositols; D000431:Ethanol; D016899:Interferon-beta; D011493:Protein Kinase C; D010738:Type C Phospholipases; D002118:Calcium