A study of variability in the MHC class II beta 1 and class I alpha 2 domain exons of Atlantic salmon, Salmo salar L.
Variability in the most extracellular exons of Atlantic salmon MHC-Sasa class I and class II was studied by polymerase chain reaction (PCR) amplification followed by sequencing. The domains studied were class I alpha 2 and class II beta 1. The material used was genomic DNA of fish, mainly derived from the major Norwegian breeding pool, supplemented with some material from a minor breeder and a local river strain. The analysis revealed extensive variation, most individuals being heterozygous with at least two variants.
Grimholt
U
U
Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, Oslo.
Olsaker
I
I
de Vries Lindstrøm
C
C
Lie
O
O
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D012474:Salmon / Q000235:genetics* / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D018076:DNA, Complementary