title: Structure of the gene for human uracil-DNA glycosylase and analysis of the promoter function.
abstract: The gene for human uracil-DNA glycosylase (UNG) contains 4 exons and has an approximate size of 13 kb. The promoter is very GC rich and lacks a TATA box. Nested deletions of the promoter demonstrated that two SP1 elements and a putative c-MYC element proximal to the transcription initiation region were sufficient to support some 27% of the promoter activity, while a clone that in addition contained the elements E2F/SP1/CCAAT increased expression to almost 90% of the full-length construct. A region upstream of these elements appears to exert a negative control function.
authors.affiliation: UNIGEN Center for Molecular Biology, University of Trondheim, Norway.
authors.forename: T
authors.initials: T
authors.lastname: Haug
authors.forename: F
authors.initials: F
authors.lastname: Skorpen
authors.forename: H
authors.initials: H
authors.lastname: Lund
authors.forename: H E
authors.initials: HE
authors.lastname: Krokan
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D004247:DNA / Q000737:chemistry*; D045647:DNA Glycosylases; D004262:DNA Restriction Enzymes; D005091:Exons; D006801:Humans; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D015183:Restriction Mapping; D016329:Sp1 Transcription Factor / Q000378:metabolism; D016385:TATA Box; D014157:Transcription Factors / Q000378:metabolism; D051981:Uracil-DNA Glycosidase
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D004247:DNA; D004262:DNA Restriction Enzymes; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase