title: Cooperative interaction of S. pombe proteins required for mating and morphogenesis.
abstract: We isolated two S. pombe genes, scd1 and scd2, that are required for normal morphology and mating. scd1 and scd2 are homologous to CDC24 and BEM1, respectively, of S. cerevisiae. Epistasis analyses indicate that scd2 and ras1 converge upon scd1, which, in turn, interacts with cdc42sp, a RHO-like GTPase. Studies with the yeast two-hybrid system indicate that scd2 forms complexes with both scd1 and cdc42sp. Furthermore, biochemical studies indicate that the interaction between scd1 and scd2 is direct. The yeast two-hybrid data further suggest that scd1, scd2, cdc42sp, and ras1, in its GTP-bound state, act cooperatively to form a protein complex.
authors.affiliation: Cold Spring Harbor Laboratory, New York 11724.
authors.forename: E C
authors.initials: EC
authors.lastname: Chang
authors.forename: M
authors.initials: M
authors.lastname: Barr
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: V
authors.initials: V
authors.lastname: Jung
authors.forename: H P
authors.initials: HP
authors.lastname: Xu
authors.forename: M H
authors.initials: MH
authors.lastname: Wigler
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004843:Epistasis, Genetic; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics*; D005816:Genetic Complementation Test; D020662:Guanine Nucleotide Exchange Factors; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009024:Morphogenesis; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; D018631:ras Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C073594:CDC24 protein, S cerevisiae; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D011518:Proto-Oncogene Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C089769:scd1 protein, S pombe; C089770:scd2 protein, S pombe; C073129:BEM1 protein, S cerevisiae; D019204:GTP-Binding Proteins; C489014:RAS1 protein, S cerevisiae; C492657:Ras1 protein, S pombe; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; D018631:ras Proteins