A role in cell-binding for the C-terminus of pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae.
Cell binding of pneumolysin to target cells is an important step in the lysis of cells by this toxin. We sought to locate the cell-binding region of pneumolysin. Deletion of the six C-terminal amino acids decreased cell-binding activity by 98%. Furthermore, mutagenesis of an amino acid near the C-terminus decreased the cell-binding activity of full-length pneumolysin by 90%. The C-terminus of pneumolysin has an important role in cell-binding activity.
Owen
R H
RH
Department of Microbiology and Immunology, University of Leicester, UK.
Boulnois
G J
GJ
Andrew
P W
PW
Mitchell
T J
TJ
D001426:Bacterial Proteins; D001665:Binding Sites; D003603:Cytotoxins / Q000737:chemistry*; D013296:Streptococcus pneumoniae / Q000378:metabolism* / Q000502:physiology; D013301:Streptolysins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D003603:Cytotoxins; D013301:Streptolysins; C032290:plY protein, Streptococcus pneumoniae