Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates.
MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode +/- 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.6% with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.
Pfaller
M A
MA
Oregon Health Sciences University, Portland.
Vu
Q
Q
Lancaster
M
M
Espinel-Ingroff
A
A
Fothergill
A
A
Grant
C
C
McGinnis
M R
MR
Pasarell
L
L
Rinaldi
M G
MG
Steele-Moore
L
L
D000666:Amphotericin B / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D003124:Colorimetry / Q000379:methods*; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D015588:Observer Variation; D015203:Reproducibility of Results
D000666:Amphotericin B; D015725:Fluconazole