Detection of genes for heat-stable enterotoxin in Escherichia coli by biotinylated ST-DNA probes.
Reference strains of enterotoxigenic Escherichia coli (ETEC), non-enterotoxigenic Escherichia coli (non-ETEC), enteropathogenic Escherichia coli (EPEC), enteroinvasive Escherichia coli (EIEC), and other enteropathogenic bacteria were used to prove the reliability of BIO-ST-DNA probe hybridization. In addition, 417 strains of E. coli isolated from children with diarrheal diseases in Shanxi Children's Hospital were examined for BIO-ST-DNA probe hybridization. In the test, BIO-ST-DNA hybridization was compared with suckling mouse assay in identifying ST-ETEC. The results obtained by both methods showed no significant difference. It was found that identification of ST-ETEC using hybridization is a simple, sensitive and more practical method.
Zhu
Q Y
QY
Shanxi Children's Hospital, Taiyuan.
Li
L Q
LQ
Lin
W M
WM
Zhou
Z J
ZJ
Liu
C J
CJ
D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial
D015342:DNA Probes; D004269:DNA, Bacterial; D004768:Enterotoxins