title: Herpes simplex virus-induced expression of 70 kDa heat shock protein (HSP70) requires early protein synthesis but not viral DNA replication.
abstract: The major 70 kDa heat shock protein (HSP70), which is scarcely expressed in unstressed rodent cells, was apparently induced by infection with herpes simplex virus (HSV). Infection with HSV types 1 and 2 elevated HSP70 mRNA levels within 4 hr post-infection. HSP70 synthesis and accumulation increased in HSV-infected cells. Irradiation of HSV with UV-light abolished the ability to induce HSP70 mRNA. Inhibitors of viral DNA synthesis did not affect the induction of HSP70 in infected cells. Protein synthesis within 2 hr after infection was necessary for HSP70 induction.
authors.affiliation: Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: E
authors.initials: E
authors.lastname: Ohgitani
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Kita
authors.forename: J
authors.initials: J
authors.lastname: Imanishi
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D004261:DNA Replication / Q000502:physiology*; D004279:DNA, Viral / Q000096:biosynthesis; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000254:growth & development / Q000502:physiology* / Q000528:radiation effects; D018258:Herpesvirus 2, Human / Q000254:growth & development / Q000502:physiology* / Q000528:radiation effects; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000502:physiology*
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D018840:HSP70 Heat-Shock Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D014764:Viral Proteins