title: Identification and characterization of a putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis.
abstract: A putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis was isolated from a lambda gt11 genomic expression library by screening with serum from a naturally infected sheep. The gene was contained in two overlapping clones, which were shown by antibody elution to encode a protein of 34 kDa in M. a. paratuberculosis. The clones were sequenced and database searches detected a motif identical to the active serine site in trypsin, and 30% homology to the putative serine proteases (HtrA proteins) of Escherichia coli, Salmonella typhimurium, Brucella abortus and Rochalimaea henselae.
authors.affiliation: Moredun Research Institute, Edinburgh, UK.
authors.forename: R M
authors.initials: RM
authors.lastname: Cameron
authors.forename: K
authors.initials: K
authors.lastname: Stevenson
authors.forename: N F
authors.initials: NF
authors.lastname: Inglis
authors.forename: J
authors.initials: J
authors.lastname: Klausen
authors.forename: J M
authors.initials: JM
authors.lastname: Sharp
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D015723:Gene Library; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics / Q000302:isolation & purification*; D012756:Sheep / Q000382:microbiology; D012757:Sheep Diseases / Q000382:microbiology; D013045:Species Specificity; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary
chemical_list: D001426:Bacterial Proteins; D012697:Serine Endopeptidases