Establishment and characterization of a novel myeloid cell line from the bone marrow of a patient with the myelodysplastic syndrome.
A novel long-term cultured myeloid cell line was established from the bone marrow of a patient with myelodysplastic syndrome (MDS). This cell line, designated MDS92, proliferated in the presence of interleukin-3 or granulocyte-macrophage colony-stimulating factor and transiently in the presence of Steel factor, with a tendency for gradual maturation, and formed myeloid colonies in the semi-solid culture condition. In addition, the MDS92 cell line represented rather complicated karyotypic abnormalities including the deletion of fifth and seventh chromosomes and a point mutation at codon 12 of the N-ras oncogene. These characteristics of the MDS92 cell line are exclusively compatible with the property of preleukaemia.
Tohyama
K
K
First Department of Internal Medicine, Fukui Medical School, Japan.
Tsutani
H
H
Ueda
T
T
Nakamura
T
T
Yoshida
Y
Y
D000756:Anemia, Sideroblastic / Q000473:pathology*; D000954:Antigens, Surface / Q000032:analysis; D001483:Base Sequence; D001853:Bone Marrow / Q000473:pathology*; D002455:Cell Division; D002460:Cell Line / Q000473:pathology*; D002869:Chromosome Aberrations; D002895:Chromosomes, Human, Pair 5; D002897:Chromosomes, Human, Pair 7; D011905:Genes, ras; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011289:Preleukemia / Q000473:pathology*; D013997:Time Factors
D000954:Antigens, Surface; D006133:Growth Substances; D007377:Interleukin-3