Cloning and characterization of a cathepsin D inhibitor gene from Solanum tuberosum L.
A DNA clone encoding a cathepsin D inhibitor CathInh was isolated from a potato genomic library using a CathInh cDNA as hybridization probe. The amino acid sequence of the coding region is nearly identical with a CathInh cDNA and CathInh proteins previously isolated from a tuber-specific cDNA library and from tubers, respectively. Analysis of GUS activity resulting from expression of chimeric CathInh promoter-GUS genes in transgenic potato plants revealed expression exclusively confined to potato tubers. No GUS activity could be detected in any other organ of the transgenic plants either constitutively or after wounding or treatment with abscisic and jasmonic acid (JA). Interestingly, part of the promoter region of the CathInh gene, essential for GUS activity in tubers, shows striking similarity to promoter regions of tuber-specific class I patatin genes.
Herbers
K
K
Institut für Genbiologische Forschung GmbH, Berlin, Germany.
Prat
S
S
Willmitzer
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D002402:Cathepsin D / Q000037:antagonists & inhibitors*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000737:chemistry; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C060481:CDI protein, Solanum tuberosum; D005966:Glucuronidase; D002402:Cathepsin D