Expression of interleukin-2 receptor gamma chain on human neutrophils.
The interleukin-2 (IL-2) receptor gamma is an indispensable functional component of IL-2, IL-4, and IL-7 receptors, and thus, is denoted the common gamma chain, gamma c. The present study was undertaken to determine whether human polymorphonuclear neutrophils (PMNs) expressed gamma c chain. Reverse transcription-polymerase chain reaction and Northern blot analysis showed that fresh human PMN constitutively expressed a remarkable level of gamma c mRNA, which is of the size and intensity of that from the peripheral blood mononuclear cells (PBMCs). Granulocyte macrophage-colony stimulating factor, IL-2, and IL-8, which are known to activate PMN functions, failed to regulate the gamma c gene expression. Western blot analysis with a rabbit anti-gamma c polyclonal antibody identified 64-, 58-, and 50-kD gamma c bands in lysates from PMN, but only 64- and 58-kD bands from PBMCs. After the PMNs and PBMCs were treated with tunicamycin to prevent N-linked glycosylation, Western blot analysis detected a single 39-kD band, which is equal to the calculated molecular weight from the cloned cDNA. Thus, our results indicate that PMNs constitutively express high levels of gamma c and the three forms detected are caused by different glycosylation of a protein translated from a single mRNA species.
Liu
J H
JH
Immunology Program, H. Lee Moffitt Cancer Center, University of South Florida College of Medicine, Tampa 33612.
Wei
S
S
Ussery
D
D
Epling-Burnette
P K
PK
Leonard
W J
WJ
Djeu
J Y
JY
D001483:Base Sequence; D005786:Gene Expression Regulation / Q000187:drug effects; D006031:Glycosylation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects; D009504:Neutrophils / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D014415:Tunicamycin / Q000494:pharmacology
D007376:Interleukin-2; D016209:Interleukin-8; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; D014415:Tunicamycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor