title: Expression and characterisation of equine herpesvirus 1 glycoprotein H using a recombinant baculovirus.
abstract: A recombinant baculovirus capable of expressing the glycoprotein H (gH) gene of equine herpesvirus 1 (EHV-1) was constructed. EHV-1 gH gene products in recombinant baculovirus infected insect cells were identified as 105 kDa and 110 kDa species compared with a 115 kDa product detected in EHV-1 infected mammalian cells. The extent of N-glycosylation of EHV-1 gH in both insect and mammalian cells was indicated by a shift in apparent molecular weights after PNGase F treatment to 90 kDa and 95 kDa forms, which compared with the predicted value of 90 kDa for the unglycosylated polypeptide. The recombinant EHV-1 gH was recognised by equine sera demonstrating that EHV-1 gH is a target for the immune system of the natural host. However, while the recombinant EHV-1 gH product from infected insect cells was immunogenic in mice, it did not induce a neutralizing antibody response against EHV-1.
authors.affiliation: School of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
authors.forename: E M
authors.initials: EM
authors.lastname: McGowan
authors.forename: M B
authors.initials: MB
authors.lastname: Hayden
authors.forename: S J
authors.initials: SJ
authors.lastname: Edwards
authors.forename: D
authors.initials: D
authors.lastname: Pye
authors.forename: D N
authors.initials: DN
authors.lastname: Love
authors.forename: J M
authors.initials: JM
authors.lastname: Whalley
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005822:Genetic Vectors; D004861:Herpesvirus 1, Equid / Q000737:chemistry*; D051379:Mice; D009500:Neutralization Tests; D017924:Nucleopolyhedroviruses; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D018411:Spodoptera; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins