Confirmation of leucogentian violet in chicken fat by gas chromatography/mass spectrometry.
A gas chromatographic/mass spectrometric (GC/MS) procedure for confirming the identity of leucogentian violet (LGV) in chicken fat was developed for regulatory application. The unused portion of the extract remaining from a determinative procedure was back-extracted into an organic phase, concentrated, and analyzed by GC/MS. Confirmation of the identity of LGV was based on matching the retention times and relative abundances of 6 ions in the extract to corresponding values obtained for the LGV standard. The procedure was validated by replicate analyses of negative control, fortified control, and residue-incurred chicken fat. The presence of LGV was confirmed by the GC/MS procedure in all samples found to contain LGV by prior liquid chromatographic analyses. There were no interferences in the control samples.
Wilson
R T
RT
U.S. Department of Agriculture, Food Safety and Inspection Service, Midwestern Laboratory, St. Louis, MO 63115.
Wong
J
J
Johnston
J
J
Epstein
R
R
Heller
D N
DN
D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D002645:Chickens; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D005840:Gentian Violet / Q000031:analogs & derivatives* / Q000032:analysis
C041914:leucogentian violet; D005840:Gentian Violet