title: Transmembrane pH of Clostridium acetobutylicum is inverted (more acidic inside) when the in vivo activity of hydrogenase is decreased.
abstract: Evidence is reported here that alkalinization of Clostridium acetobutylicum cytoplasm involves hydrogenase activity. A decrease of in vivo hydrogenase activity is accompanied by intracellular accumulation of protons leading to a negative (interior acidic) pH gradient. However, the organism is able to maintain a constant proton motive force by interconverting chemical and electrical potentials.
authors.affiliation: Centre de Ingénierie Gilbert Durand, Institut National des Sciences Appliquées, Toulouse, France.
authors.forename: L
authors.initials: L
authors.lastname: Girbal
authors.forename: I
authors.initials: I
authors.lastname: Vasconcelos
authors.forename: P
authors.initials: P
authors.lastname: Soucaille
mesh_terms: D000468:Alkalies; D003013:Clostridium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D004579:Electron Transport; D006863:Hydrogen-Ion Concentration; D006864:Hydrogenase / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D011522:Protons
chemical_list: D000468:Alkalies; D011522:Protons; D006864:Hydrogenase; D011188:Potassium