title: Vascular permeability factor accelerates endothelial regrowth following balloon angioplasty.
abstract: Many failures of vascular reconstructions are due to thrombosis and restenosis and are often attributed to inadequate endothelial regeneration at the site of endothelial denudation. Vascular permeability factor (VPF) is a naturally occurring growth factor responsible for vessel permeability and microvascular angiogenesis. Here, we show that VPF stimulated rabbit endothelial cell proliferation in vitro at concentrations 100 ng/ml. However, VPF had no effect on smooth muscle cell proliferation at these concentrations up to 500 ng/ml. When VPF was administered for 4 weeks (120 micrograms, twice weekly, i.v.) following balloon angioplasty-induced endothelial denudation of rabbit carotid artery, there was a significant increase in the in vivo regeneration of endothelium compared to control (57.5 +/- 6.7% vs. 38.3 +/- 1.9%, P < 0.01). Moreover, 8 weeks of VPF administration resulted in 88.1 +/- 3.1% re-endothelialization compared to control (44.7 +/- 3.8%). Hence, VPF appears to be a specific mitogen for endothelial regeneration.
authors.affiliation: Department of Surgery, Washington University School of Medicine, St Louis, MO 63110.
authors.forename: A D
authors.initials: AD
authors.lastname: Callow
authors.forename: E T
authors.initials: ET
authors.lastname: Choi
authors.forename: J D
authors.initials: JD
authors.lastname: Trachtenberg
authors.forename: S L
authors.initials: SL
authors.lastname: Stevens
authors.forename: D T
authors.initials: DT
authors.lastname: Connolly
authors.forename: C
authors.initials: C
authors.lastname: Rodi
authors.forename: U S
authors.initials: US
authors.lastname: Ryan
mesh_terms: D000800:Angioplasty, Balloon / Q000009:adverse effects*; D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects; D020212:Carotid Artery Injuries; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000293:injuries / Q000502:physiology; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D011651:Pulmonary Artery; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D012038:Regeneration; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D008222:Lymphokines; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors