title: Application of the one-dimensional TOCSY pulse sequence in 750 MHz 1H-NMR spectroscopy for assignment of endogenous metabolite resonances in biofluids.
abstract: The complex 1H-NMR spectrum arising from an intact biofluid has been simplified using a one-dimensional homonuclear polarization transfer experiment (known as TOCSY or HOHAHA). This approach establishes connectivity between sequentially coupled multiplets, and the method is illustrated by the confirmation of the chemical shifts and hence resonance assignment of a number of endogenous metabolites in the 750 MHz 1H-NMR spectrum of seminal fluid. This has allowed the detection and assignment of pyroglutamate and uracil in this fluid for the first time.
authors.affiliation: Bruker Analytische Messtechnik GmbH, Silberstreifen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Spraul
authors.forename: J K
authors.initials: JK
authors.lastname: Nicholson
authors.forename: M J
authors.initials: MJ
authors.lastname: Lynch
authors.forename: J C
authors.initials: JC
authors.lastname: Lindon
mesh_terms: D003198:Computer Simulation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011761:Pyrrolidonecarboxylic Acid / Q000032:analysis*; D012661:Semen / Q000378:metabolism*; D012662:Semen Preservation; D014498:Uracil / Q000032:analysis*
chemical_list: D014498:Uracil; D011761:Pyrrolidonecarboxylic Acid