title: Molecular identification of catalases from Nicotiana plumbaginifolia (L.).
abstract: We have isolated three different catalase cDNAs from Nicotiana plumbaginifolia (cat1, cat2, and cat3) and a partial sequence of a fourth catalase gene (cat4) that shows no discernible expression based on Northern analysis. The catalase sequences were used to determine the similarity with other plant catalases and to study the transcriptional response to paraquat, 3-aminotriazole, and salicylic acid. 3-Aminotriazole induces mRNA levels of cat1, cat2 and cat3, indicating that a reduction in catalase activity positively affects catalase mRNA abundance. Salicylic acid that binds catalase in vitro, had no effect on catalase transcript levels at physiological concentrations. Paraquat resulted in the induction of cat1.
authors.affiliation: Laboratorium voor Genetica, Universiteit Gent, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Willekens
authors.forename: R
authors.initials: R
authors.lastname: Villarroel
authors.forename: M
authors.initials: M
authors.lastname: Van Montagu
authors.forename: D
authors.initials: D
authors.lastname: Inzé
authors.forename: W
authors.initials: W
authors.lastname: Van Camp
mesh_terms: D000595:Amino Acid Sequence; D000640:Amitrole / Q000494:pharmacology; D001483:Base Sequence; D002374:Catalase / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016166:Free Radical Scavengers; D008969:Molecular Sequence Data; D010269:Paraquat / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000032:analysis; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D016166:Free Radical Scavengers; D010940:Plant Proteins; D012333:RNA, Messenger; D012459:Salicylates; D002374:Catalase; D002688:Chitinases; D020156:Salicylic Acid; D010269:Paraquat; D000640:Amitrole