title: Cis/trans isomerization of fatty acids as a defence mechanism of Pseudomonas putida strains to toxic concentrations of toluene.
abstract: Defence mechanisms of three Pseudomonas putida strains growing in the presence of toluene up to 50% (v/v) were investigated. The three strains reacted to toxic concentrations of toluene by accumulating trans unsaturated fatty acids in the membrane instead of the cis isomers. The membranes of the toluene-adapted cells possessed a higher trans/cis ratio and had a higher lipid-ordering since the transition temperature was about 7 centigrade degrees higher compared to the non-adapted cells.
authors.affiliation: Department of Food Science, Wageningen Agricultural University, The Netherlands.
authors.forename: F J
authors.initials: FJ
authors.lastname: Weber
authors.forename: S
authors.initials: S
authors.lastname: Isken
authors.forename: J A
authors.initials: JA
authors.lastname: de Bont
mesh_terms: D004352:Drug Resistance, Microbial; D005227:Fatty Acids / Q000737:chemistry*; D007536:Isomerism; D008968:Molecular Conformation; D016958:Pseudomonas putida / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013696:Temperature; D014050:Toluene / Q000633:toxicity*
chemical_list: D005227:Fatty Acids; D014050:Toluene