A simple procedure for assaying cAMP.
A step-by-step protocol for assaying cAMP is presented. This method is based on the standard binding protein assay that is available commercially. However, using the present procedure, the per tube cost is dramatically reduced. In the current protocol, four different binding proteins are compared for their ability to bind cAMP. The source of all reagents is noted as well as necessary precautions for insuring reliable assays. A simple tissue preparation method is outlined for assaying cAMP in brain. The utility of the assay is illustrated by demonstrating the effect of forskolin on cAMP in mouse striatal tissue.
Munirathinam
G
G
College of Pharmacy, St. John's University, Queens, NY 11439.
Yoburn
B C
BC
D000818:Animals; D002417:Cattle; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis* / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D051379:Mice; D017072:Neostriatum / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits; D011933:Reagent Kits, Diagnostic; D013268:Stimulation, Chemical; D013552:Swine
D011933:Reagent Kits, Diagnostic; D005576:Colforsin; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases