Flow cytometric analysis of phenotypes of canine red blood cells with FITC-labeled Clerodendron trichotomum lectin.
Canine red blood cells were divided into a positive type (C type) and a negative type (c type) by a agglutination test with Clerodendron trichotomum lectin (CTL). Blood cells changed from c to C type after suffering from mammary tumor were named cm. The c type blood cells treated with neuraminidase were named cn. These red blood cells were studied with flow cytometry using a directly fluoresceinated CTL. Positive percents of C type, cm type, and cn type were 49.3%, 43.8% and 81.0% respectively. While C showed one peak in histogram, cm showed two peaks. The positive peak in the cm blood cells suggested an appearance of a new blood cell population with a novel sugar chain structure after suffering from the tumor.
Usui
R
R
Department of Legal Medicine and Human Genetics, Jichi Medical School, Tochigi, Japan.
Hirota
J
J
Omi
T
T
Iwamoto
S
S
Ikemoto
S
S
D000818:Animals; D004285:Dogs / Q000097:blood*; D004912:Erythrocytes / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D016650:Fluorescein-5-isothiocyanate; D006386:Hemagglutination Tests; D037102:Lectins; D009439:Neuraminidase; D010641:Phenotype; D037121:Plant Lectins
C051213:Clerodendron trichotomum lectin; D037102:Lectins; D037121:Plant Lectins; D009439:Neuraminidase; D016650:Fluorescein-5-isothiocyanate