Comparison and assessment of the difference in total dietary fiber in cooked dried legumes as determined by five methods.
Dried beans (kidney and great northern) and dried peas (chick, green, and yellow) were cooked according to package instructions. Total dietary fiber (TDF) was measured by the Mongeau (AOAC 992.16), Prosky (AOAC 985.29), and Lee (AOAC 991.43) methods (A, B, and C, respectively). Nonstarch polysaccharides (NSP) were measured by the Englyst gas-chromatographic method that included dimethyl sulfoxide treatment, and the lignin measured separately was added to NSP (method D). TDF was also measured by the Li method (E). TDF values ranged from 7 to 48 g/100 g dry weight. Methods B and C gave similar TDF values, which were higher than those from other methods. For kidney beans I, TDF values by methods B and C were up to 2.5 times higher because of the inclusion of starch. The fiber residues from methods A and C contained the same amounts of arabinose, xylose, mannose, galactose, and uronic acid, but glucose was 4 times higher in residue C than in residue A. When alpha-amylase from porcine pancreas was incorporated in methods B or C for 5 samples, the discrepancies among methods A, B, and C were reduced by 60-98%.
Mongeau
R
R
Banting Research Centre, Nutrition Research Division, Ottawa, ON, Canada.
Brassard
R
R
D001089:Arabinose / Q000032:analysis; D002849:Chromatography, Gas; D004043:Dietary Fiber; D004121:Dimethyl Sulfoxide; D007887:Fabaceae / Q000737:chemistry*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006358:Hot Temperature; D008031:Lignin / Q000032:analysis; D008358:Mannose / Q000032:analysis; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000032:analysis; D013213:Starch / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis; D014994:Xylose / Q000032:analysis
D004043:Dietary Fiber; D011134:Polysaccharides; D014574:Uronic Acids; D013213:Starch; D008031:Lignin; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D008358:Mannose; D005690:Galactose; D004121:Dimethyl Sulfoxide