Phage display of a human antibody against Clostridium tetani toxin.
9F12, a human antibody capable of neutralising tetanus toxin in an animal model, has been expressed as a Fab fragment in a phagemid system. The nucleotide sequence for the variable domain of both chains has been determined and compared to germline sequences.
Esposito
G
G
Istituto di Ricerche di Biologia Molecolare P. Angeletti (IRBM), Pomezia (Roma), Italy.
Scarselli
E
E
Traboni
C
C
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D000918:Antibody Specificity; D001435:Bacteriophages; D001483:Base Sequence; D005822:Genetic Vectors; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013744:Tetanus Toxin / Q000276:immunology*
D000907:Antibodies, Bacterial; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D011993:Recombinant Fusion Proteins; D013744:Tetanus Toxin