title: Floral expression of a gene encoding an E2-related ubiquitin-conjugating protein from Arabidopsis thaliana.
abstract: An Arabidopsis thaliana gene (UBC6) encoding a homologue to ubiquitin-conjugating enzymes has been isolated which is capable of encoding a protein of 183 amino acids of ca. 21 kDa. Northern analysis indicates that the gene is expressed in flowers, seeds and, to a somewhat lesser extent, in 10-day seedlings but not in mature leaves, callus and pre-flowering plants. This pattern of expression is confirmed using transgenic Arabidopsis plants containing a UBC6 promoter-GUS gene fusion construct. These plants display GUS activity in mature anthers prior to dehiscence, in developing embryos, sepals and the style after pollination.
authors.affiliation: Biochemistry Department, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK.
authors.forename: F Z
authors.initials: FZ
authors.lastname: Watts
authors.forename: N
authors.initials: N
authors.lastname: Butt
authors.forename: P
authors.initials: P
authors.lastname: Layfield
authors.forename: J
authors.initials: J
authors.lastname: Machuka
authors.forename: J F
authors.initials: JF
authors.lastname: Burke
authors.forename: A L
authors.initials: AL
authors.lastname: Moore
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics; D017930:Genes, Reporter; D005966:Glucuronidase / Q000096:biosynthesis / Q000378:metabolism; D008025:Ligases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D018547:Plant Stems / Q000737:chemistry / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D044763:Ubiquitin-Conjugating Enzymes
chemical_list: D005656:Fungal Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D044763:Ubiquitin-Conjugating Enzymes; D005966:Glucuronidase; D008025:Ligases