title: [Highly-effective subtractive hybridization of cDNA].
abstract: A scheme for subtractive hybridization is described allowing for a 500-1000 fold enrichment of low abundant cDNA. The scheme is based on the previously described principle of normalization of an initial mixture of differently represented cDNAs in the single-stranded portion of a tracer after the first round of subtraction and the principle of a trapper excluding the fraction of the double-stranded cDNAs formed during the first round from the subsequent PCR-amplification. The technique is simple and makes unnecessary the separation of the tracer, driver and hybrids formed after the subtraction.
authors.forename: S A
authors.initials: SA
authors.lastname: Luk'ianov
authors.forename: N G
authors.initials: NG
authors.lastname: Gurskaia
authors.forename: K A
authors.initials: KA
authors.lastname: Luk'ianov
authors.forename: V S
authors.initials: VS
authors.lastname: Tarabykin
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary
vernacular_title: Vysokoéffektivnaia vychitaiushchaia gibridizatsiia kDNK.