title: PCR-RFLP typing of the bovine myoglobin gene.
abstract: We have identified substitutions in the 3' untranslated region of the bovine myoglobin gene, one of which affects an MboII restriction enzyme site resulting in a bi-allelic restriction fragment length polymorphism. Co-dominant inheritance of the alleles in three reference families was observed using a polymerase chain reaction--restriction fragment length polymorphism assay. The distribution of the alleles seems characteristic of cattle type--one of the alleles was not detected in purely taurine breeds. Furthermore, we mapped, using the polymerase chain reaction on a bovine-rodent somatic cell hybrid panel, the myoglobin gene to bovine chromosome five. It is therefore syntenic with gamma-interferon and insulin-like growth factor in which we have not found polymorphism. The myoglobin locus therefore serves as a type one marker on bovine chromosome five.
authors.affiliation: ILRAD, Nairobi, Kenya.
authors.forename: M
authors.initials: M
authors.lastname: Agaba
authors.forename: S J
authors.initials: SJ
authors.lastname: Kemp
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009211:Myoglobin / Q000235:genetics*; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
chemical_list: D009211:Myoglobin; D004247:DNA