DNA content in non-Hodgkin's lymphoma. Comparison between flow cytometry and cytogenetics in fresh and paraffin-embedded tissue.
DNA content of 36-non-Hodgkin's lymphomas was analyzed by flow cytometry (FCM) and cytogenetics (CG), 21 in fresh and 15 in paraffin-embedded tissue. The results of both techniques were coincident in 60% of the fresh tissue samples and in 45% of the paraffin-embedded ones, the reason for this difference could be the poor resolution of DNA histograms from paraffin-embedded tissue. All samples judged as aneuploid by FCM were aneuploid also by CG. Some samples with a hyperdiploid population by CG gave a diploid population by FCM with a 'false' high DNA-synthesis (S) fraction. From a technical point of view, CG and FCM have to be performed on the same fresh tissue.
García
R
R
Department of Immunology, Fundacion Jiménez Díaz, Madrid, Spain.
Bueno
A
A
Feijoó
E
E
Robledo
M
M
Echezarreta
G
G
Ortíz
F
F
Benítez
J
J
Sarasa
J L
JL
Rivas
C
C
D002453:Cell Cycle; D003584:Cytological Techniques; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006689:Hodgkin Disease / Q000235:genetics / Q000473:pathology*; D006801:Humans; D016612:Paraffin Embedding; D011003:Ploidies
D004273:DNA, Neoplasm