Degree of fatty acyl chain unsaturation in biliary lecithin dictates cholesterol nucleation and crystal growth.
To clarify factors involved in the formation of cholesterol gallstones, we studied the relationship between the degree of fatty acyl chain unsaturation of biliary lecithin and bile metastability. We used supersaturated model bile solutions (molar taurocholate/lecithin/cholesterol ratio (73:19.5:7.5), total lipid concentration 9 g/dl) that contained equimolar egg yolk or soybean lecithins or a sn-1 palmitoyl, sn-2 linoleoyl phosphatidylcholine. Gel permeation chromatographic studies showed that the vesicular cholesterol distribution and dimension were inversely related to the degree of unsaturation of the lecithin species, estimated by reverse phase, high-performance liquid chromatography. Differential interference contrast microscopy and assay of cholesterol crystal growth showed that a higher degree of fatty acyl chain unsaturation of the lecithin species was associated with a faster nucleation time and rate of crystal growth. Our results suggest that vesicular lecithins containing more unsaturated fatty acyl chains bind less tightly to cholesterol than lecithins containing predominantly saturated fatty acids, and that the biliary lecithin species dictates, in part, the nucleation and growth of cholesterol crystals in bile.
Tazuma
S
S
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Ochi
H
H
Teramen
K
K
Yamashita
Y
Y
Horikawa
K
K
Miura
H
H
Hirano
N
N
Sasaki
M
M
Aihara
N
N
Hatsushika
S
S
D001646:Bile / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D003460:Crystallization; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D006801:Humans; D010713:Phosphatidylcholines / Q000737:chemistry*
D005231:Fatty Acids, Unsaturated; D010713:Phosphatidylcholines; D002784:Cholesterol