Growth and regulation of enzyme synthesis in the nitrilotriacetic acid (NTA)-degrading bacterium Chelatobacter heintzii ATCC 29600.
In the aerobic bacterium Chelatobacter heintzii, growth and regulation of enzymes involved in nitrilotriacetic acid (NTA) degradation have been investigated in chemostat culture during cultivation with glucose, NTA or mixtures thereof. In batch culture mu max with NTA was 0.18 h-1 and with glucose 0.22 h-1. Growth yields for both substrates were reduced at low dilution rates. During growth with NTA specific activity of the NTA monooxygenase (NTA-MO) exhibited a maximum at D = 0.03 h-1 and gradually decreased with increasing dilution rates. In glucose-grown cells the specific activity as well as immunologically detectable NTA-MO protein was always close to the detection limit. During cultivation with different mixtures of NTA and glucose at a dilution rate of 0.06 h-1, both substrates were utilized simultaneously, irrespective of the NTA/glucose ratio and the presence of excess ammonia. Synthesis of both NTA-MO and iminodiacetic acid dehydrogenase became induced when NTA contributed to more than approximately 1-3% of the total carbon in the substrate mixture supplied. However, NTA was also degraded when the proportion of NTA in the mixture was lower than 1%, which is consistent with the low constitutive level of expression for NTA-MO observed. Results are discussed with respect to NTA biodegradation during sewage treatment and in ecosystems.
Bally
M
M
Swiss Federal Institute for Environmental Science and Technology (EAWAG), Dübendorf.
Wilberg
E
E
Kühni
M
M
Egli
T
T
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001673:Biodegradation, Environmental; D004790:Enzyme Induction; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000494:pharmacology; D006088:Gram-Negative Aerobic Bacteria / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D007098:Imino Acids / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D009571:Nitrilotriacetic Acid / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000096:biosynthesis* / Q000235:genetics; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D001426:Bacterial Proteins; D007098:Imino Acids; D000644:Quaternary Ammonium Compounds; D014874:Water Pollutants, Chemical; D006899:Mixed Function Oxygenases; C072680:nitrilotriacetate monooxygenase; D000587:Oxidoreductases Acting on CH-NH Group Donors; C089937:iminodiacetic acid dehydrogenase; C084604:nitrilotriacetate dehydrogenase; D005947:Glucose; D009571:Nitrilotriacetic Acid; C008109:iminodiacetic acid