title: CCD camera imaging for the chemiluminescent detection of enzymes using new ultrasensitive reagents.
abstract: We have designed and constructed an inexpensive imaging system based on charge-coupled device (CCD) technology and utilized it to demonstrate the sensitivity and rapid detection possible with Lumigen chemiluminescent reagents. We also report the development of two new chemiluminescent reagents, Lumi-Phos Plus and Lumigen PS. Lumi-Phos Plus is an enhanced formulation for the rapid detection of alkaline phosphatase on membranes and in solution. It provides excellent images in blotting applications with exposures of under a minute. Lumigen PS represents a new generation of peroxidase detection reagents. The wide dynamic range with excellent linearity, higher signal and lower background than other chemiluminescent reagents make Lumigen PS of unsurpassed value in enzyme-linked immunoassays and nucleic acid probe assays using HRP conjugates.
authors.affiliation: Lumigen, Inc., Southfield, MI 48034.
authors.forename: H
authors.initials: H
authors.lastname: Akhavan-Tafti
authors.forename: A P
authors.initials: AP
authors.lastname: Schaap
authors.forename: Z
authors.initials: Z
authors.lastname: Arghavani
authors.forename: R
authors.initials: R
authors.lastname: DeSilva
authors.forename: R A
authors.initials: RA
authors.lastname: Eickholt
authors.forename: R S
authors.initials: RS
authors.lastname: Handley
authors.forename: B A
authors.initials: BA
authors.lastname: Schoenfelner
authors.forename: K
authors.initials: K
authors.lastname: Sugioka
authors.forename: Y
authors.initials: Y
authors.lastname: Sugioka
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D015139:Blotting, Southern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D004247:DNA / Q000032:analysis*; D004798:Enzymes / Q000032:analysis*; D006735:Horseradish Peroxidase / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D010781:Photography / Q000379:methods*; D010783:Photometry / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D004798:Enzymes; D007202:Indicators and Reagents; D004247:DNA; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase