title: Stereological and serial section analysis of chondrocytic enlargement in the proximal tibial growth plate of the rat.
abstract: BACKGROUND
  It has been suggested that within the growth plate, the final volume and shape of hypertrophic chondrocytes are important variables in determining the rate of longitudinal bone growth. To better understand the organization and regulation of chondrocytic hypertrophy as related to longitudinal bone growth, the beginning and end, and the location and magnitude of chondrocytic volume and shape changes during the hypertrophic process were defined in the proximal tibial growth plate of 35-day-old rats.
  
  
  METHODS
  In this study we used two different approaches, a stereological analysis of chondrocytes in unbiasedly defined, narrow growth plate strata, and a serial section reconstruction and measurement of individual cells. In both experiments chondrocytes were preserved using optimal chemical fixation. Proliferating chondrocytes were identified using bromodeoxyuridine labelling, and the rate of longitudinal bone growth was determined using oxytetracycline labelling.
  
  
  RESULTS
  In both studies, immediately following cell division in the proliferative zone, chondrocytic volume gradually increased toward the mid-point of the growth plate. During this phase of about 30 hours, approximately 20% of the final cell volume was obtained. During the following 20 hours the remaining 80% was acquired. The estimated rate of cell volume increased changed from approximately 50 microns 3/hr during the first 30 hours to about 800 microns 3/hr during the last 20 hours. The increase in cell volume resulted in an increase in both the vertical and the horizontal chondrocytic diameters. Cell parameters did not change during the final five hours of the maturation process.
  
  
  CONCLUSIONS
  In this study we demonstrated that chondrocytic enlargement starts immediately following cell division in the proliferative zone, and that chondrocytic enlargement consists of two morphologically distinguishable phases. The transition point between the first and the second phase of chondrocytic enlargement corresponded with the junction between the proliferative zone and the maturation zone.
authors.affiliation: Department of Comparative Biosciences, University of Wisconsin, Madison 53706.
authors.forename: G J
authors.initials: GJ
authors.lastname: Breur
authors.forename: J
authors.initials: J
authors.lastname: Turgai
authors.forename: B A
authors.initials: BA
authors.lastname: Vanenkevort
authors.forename: C E
authors.initials: CE
authors.lastname: Farnum
authors.forename: N J
authors.initials: NJ
authors.lastname: Wilsman
mesh_terms: D000818:Animals; D001846:Bone Development / Q000502:physiology*; D002455:Cell Division; D048429:Cell Size; D006132:Growth Plate / Q000166:cytology*; D007091:Image Processing, Computer-Assisted; D008297:Male; D010780:Photogrammetry; D051381:Rats; D012016:Reference Values; D013977:Tibia / Q000166:cytology