title: Macrolide-lincosamide resistance determinants in streptococcal species isolated from the bovine mammary gland.
abstract: Seventy one streptococci isolated from dairy cows with clinical mastitis were tested for erythromycin and lincomycin susceptibility. Ten isolates (7.1%) were resistant to erythromycin and/or lincomycin and seven were constitutive and three were inducibly resistant. Nine of the isolates hybridized with one or more of the Erm probes tested and eight isolates gave PCR products with rRNA methylase primers. The Erm determinants were transferable at frequency of 10(-5) to 10(-6) per recipient.
authors.affiliation: Department of Pathobiology, University of Washington, Seattle 98195.
authors.forename: M C
authors.initials: MC
authors.lastname: Roberts
authors.forename: M B
authors.initials: MB
authors.lastname: Brown
mesh_terms: D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology*; D005260:Female; D008034:Lincomycin / Q000494:pharmacology*; D008321:Mammary Glands, Animal / Q000382:microbiology; D008414:Mastitis, Bovine / Q000382:microbiology*; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D009693:Nucleic Acid Hybridization; D013045:Species Specificity; D013291:Streptococcus / Q000187:drug effects* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004917:Erythromycin; D008034:Lincomycin