title: Protein-protein interactions between human cytomegalovirus IE2-580aa and pUL84 in lytically infected cells.
abstract: The human cytomegalovirus immediate-early protein IE2-580aa (ppUL122a) activates transcription of viral and cellular genes and represses its own transcription through sequence-specific binding to the major immediate-early promoter. In lytically infected cells, IE2-580aa interacts with a 75-kDa viral protein (p75), an early protein that is also synthesized at late times after infection. Here we show that p75 is the product of the UL84 gene. Its association with IE2-580aa in infected cells suggests that pUL84 is involved in transcription control.
authors.affiliation: Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey 17033.
authors.forename: D J
authors.initials: DJ
authors.lastname: Spector
authors.forename: M J
authors.initials: MJ
authors.lastname: Tevethia
mesh_terms: D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics*; D004261:DNA Replication; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008970:Molecular Weight; D015534:Trans-Activators; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D008562:Membrane Glycoproteins; D015534:Trans-Activators; C077406:UL115 protein, Human herpesvirus 5; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C071440:glycoprotein H, Cytomegalovirus; C499358:glycoprotein H, Human cytomegalovirus; C439068:glycoprotein O, cytomegalovirus