The homology of a novel polypeptide with stress protein characteristics in embryonic mice brain and in the hypothalamus of caloric restricted rats as determined by ultramicro western blotting.
A novel protein (p34) was observed in polyacrylamide gel fluorographs of gestation day 13 embryonic mouse brain following retinoic acid dosing of dams. Another p34 polypeptide with identical gel migratory characteristics was seen in the hypothalamus of old caloric restricted rats after "food deprivation stress". Western blotting, employing an ultramicro trans-blot cell developed in our laboratory, detected identical immunochemical determinants between these proteins, verifying their homology. Peptide mapping and Western blotting further validated the uniqueness of p34 compared with other stress proteins including heme oxygenase.
Pipkin
J L
JL
National Center for Toxicological Research, Division of Genetic Toxicology, Jefferson, AR 72079.
Hinson
W G
WG
Lyn-Cook
L E
LE
La Borde
J
J
Anson
J F
JF
Feuers
R J
RJ
Duffy
P H
PH
Hart
R W
RW
Casciano
D A
DA
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000196:embryology; D001923:Brain Chemistry / Q000502:physiology*; D002149:Energy Intake; D006360:Heat-Shock Proteins / Q000737:chemistry*; D007031:Hypothalamus / Q000737:chemistry* / Q000196:embryology; D007120:Immunochemistry; D051379:Mice; D008970:Molecular Weight; D010449:Peptide Mapping; D051381:Rats; D017386:Sequence Homology, Amino Acid
D006360:Heat-Shock Proteins