Analysis of methyl isothiocyanate in wine by gas chromatography with dual detection.
Methyl isothiocyanate (MITC) was extracted from a 5 mL wine sample with 2 mL dichloromethane, and the extract was washed with 5% sodium chloride solution. MITC was determined by gas chromatography with a dual nitrogen-phosphorus detector (NPD) and a flame photometric detector (FPD, S mode) (DB-210 column, 30 m x 0.53 mm id, column temperature, 70 degrees C). The limit of detection and quantitation of MITC with NPD-GC were 0.003 and 0.01 ppm, respectively. Average recoveries of MITC added to wine at 1 and 0.1 ppm were over 95%. This method is simple and rapid for routine analysis of MITC in wine.
Saito
I
I
Aichi Prefectural Institute of Public Health, Nagoya, Japan.
Yamada
S
S
Oshima
H
H
Ikai
Y
Y
Oka
H
H
Hayakawa
J
J
D002849:Chromatography, Gas / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006540:Herbicides / Q000032:analysis*; D017879:Isothiocyanates / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D006540:Herbicides; D017879:Isothiocyanates; C005227:methyl isothiocyanate