title: Adherence of human lens epithelial cells to conventional poly(methyl methacrylate), heparin-surface-modified, and polyHema lenses.
abstract: We developed an in vitro model to assess the adherence of human lens epithelial cells to three types of intraocular lenses: poly(methyl methacrylate) (PMMA), heparin-surface-modified PMMA (HSM-PMMA), and polyHema. Lenses were incubated with a fixed number of human lens epithelial cells. Adherent cells were counted after 72 hours in culture. Scanning electron microscopy showed significantly fewer cells adhering to the HSM-PMMA and polyHema lenses than to the PMMA lenses (P < .01). Repeat experiments on cell lines established from different donors confirmed these findings.
authors.affiliation: Department of Ophthalmology, Royal Victoria Eye and Ear Hospital, Dublin, Ireland.
authors.forename: W J
authors.initials: WJ
authors.lastname: Power
authors.forename: D
authors.initials: D
authors.lastname: Neylan
authors.forename: L M
authors.initials: LM
authors.lastname: Collum
mesh_terms: D002448:Cell Adhesion; D002452:Cell Count; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D004847:Epithelial Cells; D006493:Heparin; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology*; D007910:Lenses, Intraocular; D008689:Methacrylates; D020366:Methylmethacrylate; D008768:Methylmethacrylates
chemical_list: D008689:Methacrylates; D008768:Methylmethacrylates; D020366:Methylmethacrylate; C005044:hydroxyethyl methacrylate; D006493:Heparin