Protein kinases and protein synthesis are involved in desensitization of the plasminogen activator response of rat Sertoli cells by follicle-stimulating hormone.
Tissue-type plasminogen activator (tPA) secretion is a specific response of Sertoli cells to follicle-stimulating hormone (FSH), which is lower after preincubation of the cells with low FSH concentrations because of FSH receptor/Gs protein uncoupling. In this report, we present evidence that this desensitization induced by the lowest FSH concentrations is suppressed by specific peptidic inhibitors of endogenous PKA and PKC in permeabilized Sertoli cells. In contrast, desensitization promoted by slightly higher FSH concentrations is not mediated through PKA or PKC activation but is dependent on protein neosynthesis.
Laurent-Cadoret
V
V
Station de Physiologie de la Reproduction des Mammifères Domestiques PRMD, INRA/CNRS, URA 1291, Nouzilly, France.
Guillou
F
F
Combarnous
Y
Y
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004072:Digitonin / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008297:Male; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011506:Proteins / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000378:metabolism*
D011506:Proteins; D003994:Bucladesine; D005640:Follicle Stimulating Hormone; D003513:Cycloheximide; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D010959:Tissue Plasminogen Activator; D004072:Digitonin