title: New restriction endonuclease CviRI cleaves DNA at TG/CA sequences.
abstract: A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5'-TGCA-3' and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5'-TGCA-3' sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.
authors.affiliation: Megabase Research Products, Lincoln, NE 68503.
authors.forename: A
authors.initials: A
authors.lastname: Jin
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Xia
authors.forename: E
authors.initials: E
authors.lastname: Traylor
authors.forename: M
authors.initials: M
authors.lastname: Nelson
authors.forename: J L
authors.initials: JL
authors.lastname: Van Etten
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002708:Chlorella / Q000201:enzymology / Q000821:virology; D003596:Cytosine; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006147:Guanine; D006801:Humans; D008745:Methylation; D013379:Substrate Specificity
chemical_list: D006147:Guanine; D003596:Cytosine; D004247:DNA; C089927:endodeoxyribonuclease CviRI; D015252:Deoxyribonucleases, Type II Site-Specific