The immediate vicinity of mouse metallothionein-I gene contains two sites conferring glucocorticoid inducibility to the heterologous promoter.
Glucocorticoid responsive elements (GREs) located -252 to -209 by upstream and +1011 to +1054 bp downstream of the transcription initiation site of the mouse metallothionein-I (mMT-I) gene were identified in transient experiments. However, the promoter region of the mMT-I gene (-330 to +70 bp) was found to provide low, if any, glucocorticoid induction of the linked CAT gene, while showing strong cadmium regulation, comparable with the in vivo level.
Plisov
S Y
SY
Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk.
Nichiporenko
M G
MG
Shkapenko
A L
AL
Kumarev
V P
VP
Baranova
L V
LV
Merkulova
T I
TI
D000818:Animals; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D016384:Consensus Sequence; D003907:Dexamethasone / Q000494:pharmacology*; D004742:Enhancer Elements, Genetic / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D007739:L Cells; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D014162:Transfection
D005938:Glucocorticoids; D002104:Cadmium; D003907:Dexamethasone; D008668:Metallothionein; D015500:Chloramphenicol O-Acetyltransferase