Phosphate-regulated inactivation of the kinase PHO80-PHO85 by the CDK inhibitor PHO81.
A complex consisting of the cyclin-dependent kinase (CDK) PHO85 and the cyclin PHO80 phosphorylates and is thought to inactivate the transcription factor PHO4 when yeast cells are grown in medium containing high concentrations of phosphate. The CDK inhibitor PHO81 inhibits the kinase activity of the PHO80-PHO85 complex when Saccharomyces cerevisiae cells are grown in medium depleted of phosphate. A region of PHO81 with similarity to the mammalian CDK inhibitor p16INK4 is sufficient for inhibition in vitro. These studies demonstrate that CDK inhibitors are used to regulate kinases involved in processes other than cell cycle control and suggest that the ankyrin repeat motif may be commonly used for interaction with cyclin-CDK complexes.
Schneider
K R
KR
Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco 94143.
Smith
R L
RL
O'Shea
E K
EK
D000595:Amino Acid Sequence; D017487:Ankyrins / Q000737:chemistry; D003470:Culture Media; D018844:Cyclin-Dependent Kinases; D016213:Cyclins; D005656:Fungal Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism*; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
D017487:Ankyrins; D003470:Culture Media; D016213:Cyclins; D005656:Fungal Proteins; C066824:PHO80 protein, S cerevisiae; C081227:PHO81 protein, S cerevisiae; D010710:Phosphates; D047428:Protein Kinase Inhibitors; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D018844:Cyclin-Dependent Kinases; C058605:PHO85 protein, S cerevisiae