Cloning, sequencing and restriction fragment length polymorphism analysis of a porcine cDNA for OCT2.
A full-length pig cDNA for the POU-domain protein OCT2 has been isolated and sequenced. The 478 amino acid-long reading frame in pig OCT2 is 97% identical to human OCT2A, indicating strong conservation of function for this immunoglobulin regulatory protein. To investigate the potential use of this cDNA for mapping and identifying linkage of OCT2 to economic traits, restriction fragment length polymorphism (RFLP) analysis was used to identify a TaqI polymorphism. A population of 60 unrelated animals, as well as multigenerational families, were typed for this RFLP and showed variability in several American and Chinese breeds. The TaqI polymorphism was also detected by a non-POU-domain OCT2 probe, demonstrating that this RFLP is located in the OCT2 gene. This result suggests that OCT2 is likely to be a single-copy gene in swine as seen in other mammals.
Tuggle
C K
CK
Department of Animal Science, Iowa State University, Ames 50011.
Helm
J
J
Rothschild
M F
MF
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D014644:Genetic Variation; D006801:Humans; D007109:Immunity / Q000235:genetics; D008969:Molecular Sequence Data; D050813:Octamer Transcription Factor-2; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D013552:Swine / Q000235:genetics* / Q000276:immunology; D014157:Transcription Factors / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D050813:Octamer Transcription Factor-2; C489207:POU2F2 protein, Sus scrofa; C058435:POU2F2 protein, human; D014157:Transcription Factors