Amino-terminal blocking group and sequence of the animal fatty acid synthase.
We have cloned and sequenced the cDNA encoding the chicken fatty acid synthase. Based on the nucleotide-derived amino acid sequence of the chicken synthase, the N-terminal sequences are highly conserved among animal species, suggesting that translation of the animal synthases initiates with the same ATG codon. Like other fatty acid synthases, the NH2-terminal sequence of the chicken enzyme is blocked. We have isolated and purified the blocked NH2-terminal peptide from a tryptic digest of chicken synthase and have established that the blocking group is an acetyl group. The sequence of the native tryptic peptide confirmed the cDNA-derived amino acid sequence and suggested that all animal synthases begin with this homologous sequence. We developed simple procedures that can be used to isolate and characterize any blocked NH2-terminal peptide.
Huang
W Y
WY
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
Chirala
S S
SS
Wakil
S J
SJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018826:CD13 Antigens; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D064429:Fatty Acid Synthases / Q000737:chemistry* / Q000235:genetics; D005777:Geese; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry; D051381:Rats; D017385:Sequence Homology; D014357:Trypsin
D018076:DNA, Complementary; D010446:Peptide Fragments; D012333:RNA, Messenger; D064429:Fatty Acid Synthases; D018826:CD13 Antigens; D014357:Trypsin