title: Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation.
abstract: Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG.
authors.affiliation: Laboratorium voor Genetica, Universiteit Gent, Belgium.
authors.forename: I
authors.initials: I
authors.lastname: Ingelbrecht
authors.forename: H
authors.initials: H
authors.lastname: Van Houdt
authors.forename: M
authors.initials: M
authors.lastname: Van Montagu
authors.forename: A
authors.initials: A
authors.lastname: Depicker
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D017796:Caulimovirus; D002467:Cell Nucleus / Q000378:metabolism; D017931:DNA Primers; D004269:DNA, Bacterial / Q000378:metabolism; D004275:DNA, Ribosomal / Q000378:metabolism; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008745:Methylation; D008969:Molecular Sequence Data; D018515:Plant Leaves; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012323:RNA Processing, Post-Transcriptional; D012231:Rhizobium; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C036483:T-DNA