Specificity of autoantibodies for recombinant 60-kd and 52-kd Ro autoantigens.
OBJECTIVE
To compare patterns of Ro autoantigen recognition in primary Sjögren's syndrome (SS) and systemic lupus erythematosus (SLE).


METHODS
Sera were tested by enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation for reactivity with recombinant human 60-kd and 52-kd Ro proteins.


RESULTS
SLE and primary SS sera exhibited similar patterns of reactivity with the recombinant 52-kd protein. However, a greater proportion of sera from primary SS patients than from SLE patients did not react with recombinant 60-kd protein in ELISA yet precipitated its translation product in solution-phase assays.


CONCLUSIONS
Disease state can influence recognition of the recombinant 60-kd Ro antigen.
St Clair
E W
EW
Duke University Medical Center, Durham, NC 27710.
Burch
J A
JA
Saitta
M
M
D000974:Antibodies, Antinuclear / Q000276:immunology*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight; D020733:RNA, Small Cytoplasmic; D011994:Recombinant Proteins; D012261:Ribonucleoproteins / Q000737:chemistry / Q000276:immunology*; D000099207:SS-A Antigen
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human; C035356:SS-A antibodies