title: Rolling circle DNA replication in vitro by a complex of herpes simplex virus type 1-encoded enzymes.
abstract: Extracts of insect cells infected with baculoviruses recombinant for the herpes simplex virus 1 (HSV-1)-encoded enzymes that are required for its replication can promote the rolling circle replication of circular plasmid templates. Replication is independent of a HSV-1 origin of replication (oris) or the HSV-1 origin binding protein and is inhibited by the origin binding protein when the plasmid contains oris. Replication is dependent on a complex composed of the HSV-1-encoded DNA polymerase and its processivity enhancing factor (the UL42 protein), ICP8 (the HSV-1-encoded single-strand DNA binding protein), and the HSV-1-encoded helicase-primase. The complex can be purified by size-exclusion and anion-exchange chromatography.
authors.affiliation: Department of Biochemistry, Beckman Center, Stanford University School of Medicine, CA 94305-5307.
authors.forename: R
authors.initials: R
authors.lastname: Skaliter
authors.forename: I R
authors.initials: IR
authors.lastname: Lehman
mesh_terms: D000818:Animals; D016367:Baculoviridae; D015153:Blotting, Western; D002460:Cell Line; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004265:DNA Helicases / Q000302:isolation & purification / Q000378:metabolism; D019915:DNA Primase; D004261:DNA Replication; D004270:DNA, Circular / Q000096:biosynthesis* / Q000302:isolation & purification; D004279:DNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005090:Exodeoxyribonucleases; D016679:Genome, Viral; D018259:Herpesvirus 1, Human / Q000201:enzymology* / Q000235:genetics*; D007700:Kinetics; D010957:Plasmids; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism; D018741:Replication Origin; D018411:Spodoptera; D013698:Templates, Genetic; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C045462:ICP8 protein, Simplexvirus; D014764:Viral Proteins; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases; C043417:DNA polymerase, Simplexvirus; D004265:DNA Helicases