title: Proton resonance assignments and secondary structure of bovine angiogenin.
abstract: Angiogenins are 14-kDa proteins able to induce blood vessel growth in various preparations and are thus thought to be involved in the development of solid tumors. Angiogenins have significant similarities with extracellular ribonuclease and possess a characteristic nuclease activity against large RNA molecules. These proteins are also able to induce second-messenger pathways. We have undertaken the determination of the three-dimensional structure of bovine angiogenin by using nuclear magnetic resonance (NMR) spectroscopy. Since this protein was directly purified from cow milk, it was not possible to enrich angiogenin with 13C or 15N isotopes. However, extensive use of two-dimensional and three-dimensional proton NMR experiments enabled us to identify all but four spin systems and to assign all corresponding proton resonances. Identification of most backbone-backbone nuclear Overhauser enhancements led to the characterization of the secondary structure elements of the protein. Comparison with the structure of ribonuclease A and analysis of the location of conserved residues confirmed that the two molecules have very similar structures.
authors.affiliation: Laboratoire de Résonance Magnétique Nucléaire, Département de Chimie-Synthèse Organique, Ecole Polytechnique, France, Palaiseau.
authors.forename: C
authors.initials: C
authors.lastname: Reisdorf
authors.forename: D
authors.initials: D
authors.lastname: Abergel
authors.forename: F
authors.initials: F
authors.lastname: Bontems
authors.forename: J Y
authors.initials: JY
authors.lastname: Lallemand
authors.forename: J P
authors.initials: JP
authors.lastname: Decottignies
authors.forename: G
authors.initials: G
authors.lastname: Spik
mesh_terms: D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D011522:Protons; D012259:Ribonuclease, Pancreatic
chemical_list: D043925:Angiogenesis Inducing Agents; D011506:Proteins; D011522:Protons; C046366:angiogenin; D012259:Ribonuclease, Pancreatic