title: A comparison of serial plate agar dilution, Bauer-Kirby disk diffusion, and the Vitek AutoMicrobic system for the determination of susceptibilities of Klebsiella spp., Enterobacter spp., and Pseudomonas aeruginosa to ten antimicrobial agents.
abstract: The use of rapid, automated technologies for assessment of antimicrobial susceptibility and determination of minimum inhibitory concentrations has been evolving for over a decade. We compared the Vitek AutoMicrobic system and Bauer-Kirby disk diffusion with the National Committee for Clinical Laboratory Standards methods of serial plate agar dilution for qualitative and quantitative susceptibilities of 301 hospital isolates of Klebsiella spp., Enterobacter spp., and Pseudomonas aeruginosa. Antibiotics tested were aztreonam, cefoperazone, ceftazidime, ceftriaxone, ciprofloxacin, gentamicin, imipenem, piperacillin, ticarcillin-clavulanic acid, and tobramycin. Agar dilution and Bauer-Kirby results were more strongly correlated for all three genera than were the results for agar dilution and Vitek. If agar dilution is presumed to be the "gold standard," Bauer-Kirby disk diffusion had only half the number of false susceptibles as did the Vitek. Thus, the Vitek AutoMicrobic system seems to be somewhat less reliable for both qualitative and quantitative measurement of susceptibility and resistance than is Bauer-Kirby disk diffusion.
authors.affiliation: Temple University Health Sciences Center Department of Medicine, Philadelphia, PA 19140.
authors.forename: T
authors.initials: T
authors.lastname: Fekete
authors.forename: H
authors.initials: H
authors.lastname: Tumah
authors.forename: J
authors.initials: J
authors.lastname: Woodwell
authors.forename: A
authors.initials: A
authors.lastname: Truant
authors.forename: V
authors.initials: V
authors.lastname: Satishchandran
authors.forename: P
authors.initials: P
authors.lastname: Axelrod
authors.forename: B
authors.initials: B
authors.lastname: Kreter
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004754:Enterobacter / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D007709:Klebsiella / Q000187:drug effects* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D000362:Agar