title: CCAAT/enhancer-binding proteins alpha and beta in brown adipose tissue: evidence for a tissue-specific pattern of expression during development.
abstract: CCAAT/enhancer-binding protein (C/EBP) alpha mRNA and its protein products C/EBP alpha and 30 kDa C/EBP alpha are expressed in rat brown-adipose tissue. Results also demonstrate the expression of C/EBP beta mRNA and its protein products C/EBP beta and liver inhibitory protein (LIP) in the tissue. The abundance of C/EBP alpha and C/EBP beta proteins in adult brown fat is similar to that found in adult liver. However, the expression of C/EBP alpha and C/EBP beta is specifically regulated in brown fat during development. C/EBP alpha, 30 kDa C/EBP alpha, C/EBP beta and LIP content is several-fold higher in fetal brown fat than in the adult tissue, or liver at any stage of development. Peak values are attained in late fetal life, in concurrence with the onset of transcription of the uncoupling protein (UCP) gene, the molecular marker of terminal brown-adipocyte differentiation. When adult rats are exposed to a cold environment, which is a physiological stimulus of brown-adipose tissue hyperplasia and UCP gene expression, a specific rise in C/EBP beta expression with respect to C/EBP alpha, 30 kDa C/EBP alpha and LIP is observed. Present data suggest that the C/EBP family of transcription factors has an important role in the development and terminal differentiation of brown-adipose tissue.
authors.affiliation: Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Manchado
authors.forename: P
authors.initials: P
authors.lastname: Yubero
authors.forename: O
authors.initials: O
authors.lastname: Viñas
authors.forename: R
authors.initials: R
authors.lastname: Iglesias
authors.forename: F
authors.initials: F
authors.lastname: Villarroya
authors.forename: T
authors.initials: T
authors.lastname: Mampel
authors.forename: M
authors.initials: M
authors.lastname: Giralt
mesh_terms: D002001:Adipose Tissue, Brown / Q000196:embryology / Q000378:metabolism*; D000818:Animals; D001119:Arginase / Q000096:biosynthesis; D015152:Blotting, Northern; D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005314:Embryonic and Fetal Development / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D007473:Ion Channels; D008099:Liver / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D024101:Mitochondrial Proteins; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors; D000071256:Uncoupling Protein 1
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D000071256:Uncoupling Protein 1; D001119:Arginase