title: CRP induces the repositioning of MalT at the Escherichia coli malKp promoter primarily through DNA bending.
abstract: Activation of transcription initiation at the Escherichia coli malKp promoter requires the repositioning of MalT, the primary activator, from a set of non-productive sites to a set of productive sites, which is staggered by 3 bp. Occupation of the latter relies on the formation of a higher order structure involving distal MalT sites and the binding of CRP (cAMP receptor protein) to three sites located in the intervening region. We show here that one can successfully replace all of the CRP sites by the binding site of another DNA-bending protein, integration host factor, or by a sequence-directed bend without altering the process of malKp activation. This observation indicates that CRP action at malKp does not involve critical interactions with MalT and that CRP promotes MalT repositioning primarily through DNA bending. This structural role of CRP differs markedly from its role in the activation of the lac promoter.
authors.affiliation: Unité de Génétique Moléculaire, URA CNRS 1149, Institut Pasteur, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Richet
authors.forename: L
authors.initials: L
authors.lastname: Søgaard-Andersen
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism* / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites; D002373:Cyclic AMP Receptor Protein / Q000502:physiology*; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D034982:Integration Host Factors; C058976:MalT protein, E coli; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors