Degradation and disposal of some enzyme inhibitors. Scientific note.
Five enzyme inhibitors (phenylmethylsulfonyl fluoride, 4-amidinophenylmethanesulfonyl fluoride, 4-(2-aminoethyl)benzenesulfonyl fluoride, N alpha-p-tosyl-L-lysine chloromethyl ketone, and N-tosyl-L-phenylalanine chloromethyl ketone) in buffer, DMSO, or stock solutions were completely degraded by adding 1M NaOH and the final reaction mixtures were not mutagenic. The stability of these compounds decreased as the pH increased.
Lunn
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Program Resources, Inc./DynCorp, Environmental Control and Research Program, NCI-Frederick Cancer Research and Development Center, MD 21702-1201.
Sansone
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EB
D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004791:Enzyme Inhibitors / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009153:Mutagens; D010664:Phenylmethylsulfonyl Fluoride / Q000737:chemistry; D012972:Sodium Hydroxide / Q000494:pharmacology; D014105:Tosyl Compounds / Q000737:chemistry; D014107:Tosyllysine Chloromethyl Ketone / Q000737:chemistry; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000737:chemistry
D004791:Enzyme Inhibitors; D009153:Mutagens; D014105:Tosyl Compounds; D014107:Tosyllysine Chloromethyl Ketone; D014108:Tosylphenylalanyl Chloromethyl Ketone; D012972:Sodium Hydroxide; D010664:Phenylmethylsulfonyl Fluoride