title: Effect of dietary vitamin C and catalase inhibition of antioxidants and molecular markers of oxidative damage in guinea pigs.
abstract: Guinea pigs were fed for five weeks with two diets with different levels of vitamin C, low (33 mg of Vit C/Kg diet) and high (13,200 mg of Vit C/Kg of diet). Catalase was inhibited with 3-amino-1,2,4-triazole (AT) in half of the animals from each dietary group. AT caused an almost complete depletion of liver catalase activity (90%) in both dietary groups. Vitamin C supplementation increased total glutathione peroxidase activity and tissue vitamin C level and decreased levels of protein carbonyls and malondialdehyde (MDA) in both treated and non-treated animals. This vitamin C supplementation did not change any of the other antioxidant defences studied. Our results show that dietary vitamin C supplementation increases global antioxidant capacity and decreases endogenous oxidative damage in the guinea pig liver under normal non-stressful conditions. This supports the protective value of dietary antioxidant supplementation.
authors.affiliation: Department of Animal Biology II (Animal Physiology), Faculty of Biology, Complutense University, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Cadenas
authors.forename: C
authors.initials: C
authors.lastname: Rojas
authors.forename: R
authors.initials: R
authors.lastname: Pérez-Campo
authors.forename: M
authors.initials: M
authors.lastname: López-Torres
authors.forename: G
authors.initials: G
authors.lastname: Barja
mesh_terms: D000640:Amitrole / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D002374:Catalase / Q000037:antagonists & inhibitors*; D004032:Diet; D005979:Glutathione Peroxidase / Q000378:metabolism; D006168:Guinea Pigs; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D001205:Ascorbic Acid; D000640:Amitrole