The human immunodeficiency virus type 1 Rev protein shuttles between the cytoplasm and nuclear compartments.
A retroviral regulatory protein, Rev (regulator of virion protein expression), is made in cells infected by human immunodeficiency virus (HIV). Rev is essential for the completion of the retroviral life cycle and interacts with the host cell at some posttranscriptional step in order to express the incompletely spliced HIV mRNAs from which HIV structural proteins are translated. Neither the host cell components nor the mechanisms responsible for this important regulation have been defined. We now report that Rev is a nucleocytoplasmic shuttle protein which is continuously transported between the cytoplasm, the nucleoli, and nucleoplasmic speckles enriched in RNA splicing and processing factors. The results show that Rev has the potential to interfere specifically with the splicing of the HIV pre-mRNA in the nucleoplasm and, next, guide such mRNAs to the cytoplasm for translation.
Kalland
K H
KH
Department of Microbiology and Immunology, Gade Institute, Bergen High Technology Centre, University of Bergen, Norway.
Szilvay
A M
AM
Brokstad
K A
KA
Saetrevik
W
W
Haukenes
G
G
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001693:Biological Transport, Active / Q000187:drug effects; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D015694:Gene Products, rev / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015483:HIV Antibodies; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D012326:RNA Splicing; D054321:rev Gene Products, Human Immunodeficiency Virus
D000911:Antibodies, Monoclonal; D015694:Gene Products, rev; D015483:HIV Antibodies; D010446:Peptide Fragments; D054321:rev Gene Products, Human Immunodeficiency Virus; D003609:Dactinomycin