title: Expression of F4, 8.12, 3I, and 16/6 anti-DNA idiotype-related antigens on cationic human IgG myeloma proteins.
abstract: One hundred cationic isolated human IgG myeloma proteins were studied for expression of four anti-DNA idiotypic (Id) markers F4, 3I, 8.12, and 16/6. Forty-three of 100 myelomas showed the presence of at least one anti-DNA idiotype. Twenty-seven were F4 positive, 18 were 16/6 positive, 8 were 3I positive, and 7 were positive for 8.12. Two different anti-DNA idiotypic markers were found together on the same 14 myeloma proteins and 3 myelomas showed three different anti-DNA Ids. Anti-DNA activity was found on only 1 of 100 myelomas but not associated with presence of an anti-DNA Id. Ten myelomas showed anti-F(ab')2 activity and 6 of these also showed the presence of an anti-DNA Id marker. When myeloma proteins expressing anti-DNA Ids were compared in direct competition ELISAs with known Id-positive human IgG anti-DNA antibodies, much less inhibition was recorded in comparison to known human anti-DNA antibodies. Our findings indicate that human IgG myeloma proteins may show positive reactions with anti-DNA idiotypic antibodies but do not express the complete anti-DNA idiotypic antigenic complex.
authors.affiliation: Department of Medicine, University of Florida School of Medicine, Gainesville 32610.
authors.forename: R C
authors.initials: RC
authors.lastname: Williams
authors.forename: A D
authors.initials: AD
authors.lastname: Schriber
authors.forename: C C
authors.initials: CC
authors.lastname: Malone
authors.forename: F
authors.initials: F
authors.lastname: Silvestris
authors.forename: N
authors.initials: N
authors.lastname: Hannigan
authors.forename: M
authors.initials: M
authors.lastname: Klein-Gitelman
authors.forename: C
authors.initials: C
authors.lastname: Namyst
authors.forename: R A
authors.initials: RA
authors.lastname: Kyle
mesh_terms: D000974:Antibodies, Antinuclear / Q000502:physiology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000032:analysis; D002412:Cations; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D009194:Myeloma Proteins / Q000737:chemistry / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002412:Cations; D007130:Immunoglobulin Idiotypes; D009194:Myeloma Proteins