title: Relation between platinum-DNA adducts and complete remission in adult acute nonlymphocytic leukemia.
abstract: The purpose of this study was to determine the relationship between platinum-DNA adducts in leukemic cells and the success of remission induction therapy in adult patients with acute nonlymphocytic leukemia (ANLL). Freshly isolated cells from pre-treatment bone marrow aspirates of 14 patients were incubated with cisplatin in vitro and the amount of platinum bound to DNA was determined using a competitive ELISA procedure and an antibody directed against platinum-modified DNA. Platinum-DNA adduct levels discriminated well between complete remission (CR) and remission failure (RF) patients, treated with mitoxantrone, cytosine arabinoside +/- carboplatin.
authors.affiliation: Ontario Cancer Treatment and Research Foundation, Hamilton, Canada.
authors.forename: S G
authors.initials: SG
authors.lastname: Armstrong
authors.forename: G P
authors.initials: GP
authors.lastname: Browman
authors.forename: A M
authors.initials: AM
authors.lastname: Benger
authors.forename: R M
authors.initials: RM
authors.lastname: Meyer
authors.forename: K L
authors.initials: KL
authors.lastname: McKay
authors.forename: G
authors.initials: G
authors.lastname: Singh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002945:Cisplatin / Q000378:metabolism*; D003561:Cytarabine / Q000008:administration & dosage; D018736:DNA Adducts / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D008942:Mitoxantrone / Q000008:administration & dosage; D009944:Organoplatinum Compounds / Q000032:analysis* / Q000378:metabolism; D011237:Predictive Value of Tests; D012008:Recurrence; D012074:Remission Induction
chemical_list: C065354:1,2-cyclohexanediamine platinum(II)-DNA complex; D018736:DNA Adducts; D009944:Organoplatinum Compounds; D003561:Cytarabine; D008942:Mitoxantrone; D002945:Cisplatin