title: Liquid chromatographic determination of ceftibuten, a new oral cephalosporin, in human plasma and urine.
abstract: Two liquid chromatographic methods with UV detection were developed for the determination of ceftibuten in human plasma and urine. Diluted plasma samples were directly injected onto a reversed-phase column without prior protein precipitation while diluted urine samples were processed through an automated on-line sample clean-up procedure using column-switching. Both methods were linear over clinically relevant concentration ranges in plasma (from 0.1 to 50 micrograms ml-1) and urine (from 0.5 to 60 micrograms ml-1). The methods showed acceptable precision (RSD < 20%) and accuracy (bias < 15%) at the limit of quantitation (LOQ) for ceftibuten in plasma and urine. These LOQs represented the lowest concentrations of ceftibuten in plasma (0.1 micrograms ml-1) and urine (0.5 micrograms ml-1) that could be measured with acceptable precision and accuracy. RSDs for both within-day and between-day analyses were < or = 12% for plasma and < 7% for urine. These methods have been used successfully for the analysis of ceftibuten in plasma and urine following single oral doses of 200, 400 and 800 mg in man.
authors.affiliation: Schering-Plough Research Institute, Department of Drug Metabolism and Pharmacokinetics, Kenilworth, NJ 07033.
authors.forename: J M
authors.initials: JM
authors.lastname: Lim
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: A
authors.initials: A
authors.lastname: Marco
authors.forename: P
authors.initials: P
authors.lastname: Mojaverian
authors.forename: C C
authors.initials: CC
authors.lastname: Lin
mesh_terms: D000284:Administration, Oral; D000077722:Ceftibuten; D002511:Cephalosporins / Q000008:administration & dosage / Q000097:blood* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D002511:Cephalosporins; D000077722:Ceftibuten