Microsomal glutathione S-transferase (GST) isozymes in Aspergillus ochraceus TS: induction by 3-methylcholanthrene.
Induction of microsomal glutathione S-transferase isozymes in Aspergillus ochraceus TS is reported. Among the inducers of mammalian drug-metabolising enzymes 3-methylcholanthrene, benzo(a)pyrene and polychlorinated biphenyl enhanced the microsomal GST activity quite considerably. On the other hand phenobarbital, beta-naphthoflavone and trans-stilbene oxide were almost without any effect. To our knowledge this seems to be the first report on induction of microsomal GST in lower eukaryotes. A significant increase (7.9 fold) in GST activity was observed with the cells induced by 3-methylcholanthrene. Moreover, three new GST isozymes were found to be present in 3-methylcholanthrene induced microsomes. The isozymes are basic in nature. The GST isozymes were found to be catalytically distinct from each other.
Datta
J
J
Department of Microbiology, Bose Institute, Calcutta, India.
Dutta
T K
TK
Samanta
T B
TB
D001235:Aspergillus ochraceus / Q000187:drug effects / Q000201:enzymology*; D004790:Enzyme Induction; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000302:isolation & purification; D007527:Isoenzymes / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D008748:Methylcholanthrene / Q000494:pharmacology*; D008861:Microsomes / Q000201:enzymology*; D013379:Substrate Specificity
D007527:Isoenzymes; D008748:Methylcholanthrene; D005982:Glutathione Transferase