title: Diagnostic test for lymphoproliferative disease virus infection of turkeys, using the polymerase chain reaction.
abstract: Lymphoproliferative disease virus, a type-C retrovirus, is the etiologic agent of a naturally acquired lymphoproliferative disorder in turkeys. The disease is characterized by rapid induction of lymphoproliferative lesions with morphologic and histopathologic features resembling those of reticuloendotheliosis. Owing to lack of overt clinical manifestations, early detection of lymphoproliferative disease virus is essential for preventing the rapid horizontal spread of the disease that can decimate flocks. We describe development of a simple, rapid, sensitive, and highly specific assay, using the polymerase chain reaction, capable of providing differential diagnosis of the disease soon after infection.
authors.affiliation: Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Sarid
authors.forename: A
authors.initials: A
authors.lastname: Chajut
authors.forename: M
authors.initials: M
authors.lastname: Malkinson
authors.forename: S R
authors.initials: SR
authors.lastname: Tronick
authors.forename: A
authors.initials: A
authors.lastname: Gazit
authors.forename: A
authors.initials: A
authors.lastname: Yaniv
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D018142:Gammaherpesvirinae / Q000235:genetics* / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D014422:Turkeys
chemical_list: D017931:DNA Primers; D004279:DNA, Viral