Prolyl hydroxylase inhibitor and lysyl hydroxylase inhibitor inhibit spreading of corneal epithelium.
BACKGROUND
The spreading of epithelium is critical in the healing of corneal wounds. Such epithelial spreading requires the continuous production of protein and glycoprotein. To determine whether collagen production is required for the spreading of corneal epithelium, we studied the effects of inhibitors for collagen production on spreading of corneal epithelium in vitro.


METHODS
We examined the effect of two proline analogs, L-azetidine 2-carboxylic acid and cis-hydroxyproline, a prolyl hydroxylase inhibitor, ethyl-3,4-dihydroxybenzoate, and a lysyl hydroxylase inhibitor, minoxidil, on the spreading of epithelium of organ-cultured rabbit cornea.


RESULTS
Both analogs and inhibitors inhibited epithelial spreading in a dose-dependent manner.


CONCLUSIONS
These observations indicate that collagen production may be involved in the spreading of corneal epithelium.
Saika
S
S
Department of Ophthalmology, Wakayama Medical College, Japan.
Hashizume
N
N
Okada
Y
Y
Kobata
S
S
Yamanaka
O
O
Uenoyama
K
K
Ooshima
A
A
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000502:physiology; D003315:Cornea / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000502:physiology; D062385:Hydroxybenzoates / Q000494:pharmacology; D008914:Minoxidil / Q000494:pharmacology; D009924:Organ Culture Techniques; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000037:antagonists & inhibitors*; D011393:Procollagen-Proline Dioxygenase / Q000037:antagonists & inhibitors*; D011392:Proline / Q000031:analogs & derivatives; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011817:Rabbits
D062385:Hydroxybenzoates; D011500:Protein Synthesis Inhibitors; C018063:ethyl protocatechuate; D008914:Minoxidil; D003094:Collagen; D011392:Proline; D011393:Procollagen-Proline Dioxygenase; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase