Leucocyte migration inhibition index responses of patients of amoebic liver abscess to various antigens of Entamoeba histolytica.
The whole amoebic lysate (WAL), Sephadex G-200 eluted fraction-I. (F-I), detergent dissected membrane proteins (DDMP), amoebic membrane glycoprotein (AMG) and amoebal RNA were extracted from E. histolytica. Amoebal RNA recognized 98.21% where as F-I, AMG, DDMP and WAL recognised 92.85, 91.07, 89.28 and 75% of total 56 clinically proven and amoebic serology positive amoebic liver abscess cases respectively. Intensity of recognition of individual fraction i.e., leucocyte migration inhibition index of each fraction was found to be highly significant (P < 0.001) when compared to whole amoebic lysate. This indicate the leucocyte migration inhibition index responses of patients of amoebic liver abscess to various antigenic fractions of E. histolytica with variations.
Nayeem
M A
MA
Department of Gastroenterology, Osmania General Hospital, Osmania Medical College, Hyderabad, India.
Habibullah
C M
CM
D000328:Adult; D000818:Animals; D000940:Antigenic Variation; D000953:Antigens, Protozoan / Q000097:blood*; D002464:Cell Migration Inhibition; D004748:Entamoeba histolytica / Q000276:immunology*; D005260:Female; D006801:Humans; D008101:Liver Abscess, Amebic / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D000953:Antigens, Protozoan