Molecular cloning and characterization of a cDNA for bovine phospholipase C-alpha: proposal of redesignation of phospholipase C-alpha.
We have isolated bovine phospholipase C (PLC)-alpha cDNA from bovine thymus. Sequence analysis showed that PLC-alpha is highly conserved among rat, mouse, and calf and that it has two Trp-Cys-Gly-His-Cys-Lys motifs completely conserved in the mammals. Southern blot analysis revealed that bovine PLC-alpha is derived from a single gene. When PLC-alpha cDNA was stably transfected in NIH3T3 cells, there was no increase in PLC activity. PLC-alpha is supposed to be a member not of PLC superfamily but of Trp-Cys-Gly-His-Cys-Lys motif-containing proteins consisting of protein disulfide isomerase, P5, ERp72, and thioredoxin. PLC-alpha should be redesignated ERp57 (ER-resided p57).
Hirano
N
N
Third Department of Internal Medicine, University of Tokyo, Japan.
Shibasaki
F
F
Kato
H
H
Sakai
R
R
Tanaka
T
T
Nishida
J
J
Yazaki
Y
Y
Takenawa
T
T
Hirai
H
H
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000378:metabolism; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013950:Thymus Gland / Q000201:enzymology*; D014162:Transfection; D010738:Type C Phospholipases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D007527:Isoenzymes; D010738:Type C Phospholipases