title: The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats.
abstract: In plants, resistance to a pathogen is frequently correlated with a genetically defined interaction between a plant resistance gene and a corresponding pathogen avirulence gene. A simple model explains these gene-for-gene interactions: avirulence gene products generate signals (ligands), and resistance genes encode cognate receptors. The A. thaliana RPS2 gene confers resistance to the bacterial pathogen P. syringae carrying the avirulence gene avrRpt2. A map-based positional cloning strategy was used to identify RPS2. The identification of RPS2 was verified using a newly developed transient assay for RPS2 function and by genetic complementation in transgenic plants. RPS2 encodes a novel 105 kDa protein containing a leucine zipper, a nucleotide-binding site, and 14 imperfect leucine-rich repeats.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts.
authors.forename: M
authors.initials: M
authors.lastname: Mindrinos
authors.forename: F
authors.initials: F
authors.lastname: Katagiri
authors.forename: G L
authors.initials: GL
authors.lastname: Yu
authors.forename: F M
authors.initials: FM
authors.lastname: Ausubel
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins; D001483:Base Sequence; D001665:Binding Sites; D016386:Chromosome Walking; D003001:Cloning, Molecular; D017343:Genes, Plant / Q000235:genetics*; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D007113:Immunity, Innate / Q000235:genetics; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D009711:Nucleotides / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011552:Pseudomonas Infections; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D009711:Nucleotides; D010940:Plant Proteins; C089426:RPS2 protein, Arabidopsis