[Highly-effective subtractive hybridization of cDNA].
A scheme for subtractive hybridization is described allowing for a 500-1000 fold enrichment of low abundant cDNA. The scheme is based on the previously described principle of normalization of an initial mixture of differently represented cDNAs in the single-stranded portion of a tracer after the first round of subtraction and the principle of a trapper excluding the fraction of the double-stranded cDNAs formed during the first round from the subsequent PCR-amplification. The technique is simple and makes unnecessary the separation of the tracer, driver and hybrids formed after the subtraction.
Luk'ianov
S A
SA
Gurskaia
N G
NG
Luk'ianov
K A
KA
Tarabykin
V S
VS
Sverdlov
E D
ED
D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D018076:DNA, Complementary
Vysokoéffektivnaia vychitaiushchaia gibridizatsiia kDNK.