[Study on morphology and acid secretion in cultured-parietal cells from rabbit gastric mucosa].
In order to investigate the morphological characteristics and functions of gastric parietal cells, excluding factors which affect acid secretion, parietal cells were isolated from a rabbit's gastric mucosa and cultured for several days. The long-term effects of H2-receptor antagonists on morphological characteristics and functions of the parietal cells were also investigated, using primary-cultured cells. Immediately after isolation, the parietal cells showed morphological diversity, especially in terms of the numbers and the sizes of secretory canaliculi as seen by electron microscopy. Forty-eight hours after implantation, the morphological characteristics of the cultured-parietal cells showed homogeneity, characterized by the round-shape, a few of the slightly dilated secretory canaliculi around the nucleus. When stimulated by histamine, cultured-parietal cells displayed remarkably dilated secretory canaliculi with a special type of margin on the surroundings of the canaliculi 5 min after the stimulation took place. Acid secretion from cultured-parietal cells was almost the same as that of freshly-isolated parietal cells when stimulated by histamine up to, at least, 72 hours after implantation. Cultured-parietal cells exhibited acid secretion after stimulation by carbachol, but showed no response for gastrin, the same as freshly-isolated parietal cells. When isolated parietal cells were cultured for 48 hours with cimetidine, cell viability measured by MTT assay was significantly higher than those cultured without cimetidine. However in morphological examination, the primary culture of parietal cells with three types of H2-blocker (cimetidine, ranitidine, famotidine) induced same damage to the cells. Our results indicate that primary culture of isolated parietal cells is a useful method for the study of morphology and the functions of parietal cells, because of homogeneity in morphology and sustained function of acid secretion for, at least, 72 hours. It is also a useful method for the investigation of long-term effects of drugs which specifically affect parietal cells.
Ohtaki
T
T
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology* / Q000187:drug effects; D006635:Histamine H2 Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D008854:Microscopy, Electron; D010295:Parietal Cells, Gastric / Q000378:metabolism* / Q000648:ultrastructure; D011817:Rabbits
D006635:Histamine H2 Antagonists