title: Site-directed mutagenesis using a uracil-containing phagemid template.
authors.affiliation: Molecular Genetics Unit, MRC Centre, Cambridge, UK.
authors.forename: M K
authors.initials: MK
authors.lastname: Trower
mesh_terms: D001483:Base Sequence; D045647:DNA Glycosylases; D004274:DNA, Recombinant / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009699:N-Glycosyl Hydrolases / Q000172:deficiency; D011755:Pyrophosphatases / Q000172:deficiency; D013698:Templates, Genetic; D014498:Uracil; D051981:Uracil-DNA Glycosidase
chemical_list: D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D014498:Uracil; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase