title: Resistance to degradation by nucleases of (2'S)-2'-deoxy-2'-C-methyloligonucleotides, novel potential antisense probes.
abstract: An oligonucleotide must fulfill two main requirements to become a potential antisense probe: effective hybridization properties with the complementary sequence and stability toward nucleases. In this article the degradation pattern of a new class of potential antisense fragment, (2'S)-2'-deoxy-2'-C-methyloligonucleotides, is analyzed. The results described here show that the modification introduced in these oligonucleotides confers an enhanced stability toward purified nucleases, human sera, and HeLa cell extracts.
authors.affiliation: Istituto di Richerche di Biologia Molecolare P. Angeletti, Pomezia, Rome, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Iribarren
authors.forename: D O
authors.initials: DO
authors.lastname: Cicero
authors.forename: P J
authors.initials: PJ
authors.lastname: Neuner
mesh_terms: D001483:Base Sequence; D003851:Deoxyribonucleases / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry*
chemical_list: D016376:Oligonucleotides, Antisense; D003851:Deoxyribonucleases; D012260:Ribonucleases