New rapid assay for methicillin by spectrofluorimetry in pharmaceutical dosage forms.
A method for the spectrofluorimetric determination of methicillin is proposed (lambda ex = 279 nm, lambda em = 379 nm), for concentrations between 0.030 and 10.0 micrograms ml-1. The method was performed in water, at pH 6.20 provided by addition of phosphate buffer solution. The values obtained for detection and determination limits were 0.0188 and 0.063 micrograms ml-1, respectively. The method was successfully applied to assay a commercial injection containing methicillin sodium monohydrate.
Murillo
J A
JA
Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Ciudad Real, Spain.
Alañón
A
A
D002138:Calibration; D004304:Dosage Forms; D006863:Hydrogen-Ion Concentration; D008712:Methicillin / Q000032:analysis*; D010710:Phosphates / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D004304:Dosage Forms; D010710:Phosphates; D008712:Methicillin