Antitumor immunity induced by photodynamic therapy with aluminum disulfonated phthalocyanines and laser light.
Photodynamic therapy (PDT) is systemic administration of tumor localizing photosensitizers and subsequent irradiation with light of the appropriate wavelength. The combination of drug uptake in malignant tissues and selective delivery of laser-generated light provides effective therapy, with efficient tumor cytotoxicity and minimal normal tissue damage. There have been few studies of the effects of photoactivated photosensitizers on the host immune response. Since immunity is important in the control of tumor growth and spread, we have examined, in our laboratory, the effects of photoactivated phthalocyanines on the antitumor immune response. Immunosuppressed and normal mice bearing the MS-2 fibrosarcoma treated with 5 mg/kg of aluminum disulfonated phthalocyanine (AIS2Pc) and then the tumor mass exposed to laser light (100 mW/cm2 x 10 min) or treated with surgical excision of the tumor survived indefinitely, with no difference between the different groups. The survivors, tumor-free 100 days after the treatment modalities described above, were rechallenged with the parental MS-2. Some groups of surviving animals were immunosuppressed with cyclophosphamide before the injection of the tumor. Resistance to rechallenge was evidenced only in normal surviving animals cured by PDT, while the immunodepressed surviving animals and animals cured by surgery died of tumor. Finally, mice, cured by PDT and tumor-free, rechallenged with L1210 and P388 murine leukemias did not survive. These results suggest that a potential and specific 'antitumor immunity' is induced by PDT with photoactivated AIS2Pc.
Canti
G
G
Department of Pharmacology, School of Medicine, University of Milano, Italy.
Lattuada
D
D
Nicolin
A
A
Taroni
P
P
Valentini
G
G
Cubeddu
R
R
D000818:Animals; D002455:Cell Division / Q000502:physiology; D005354:Fibrosarcoma / Q000276:immunology / Q000601:surgery / Q000628:therapy; D007211:Indoles / Q000627:therapeutic use*; D053685:Laser Therapy; D007939:Leukemia L1210 / Q000276:immunology / Q000601:surgery / Q000628:therapy; D007941:Leukemia P388 / Q000276:immunology / Q000601:surgery / Q000628:therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000601:surgery / Q000628:therapy*; D009942:Organometallic Compounds / Q000627:therapeutic use*; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents / Q000627:therapeutic use*
D007211:Indoles; D009942:Organometallic Compounds; D011838:Radiation-Sensitizing Agents; C064331:aluminum phthalocyanine disulfonate