title: Secondary-sites for integration mediated by the Tn21 integrase.
abstract: The integrase encoded by the integron of the transposon Tn21 can mediate the site-specific fusion of two plasmids if there is a recombination hot spot (59 bp element) in one of them and the sequence GWTMW in the other. The use of this latter, loosely defined site explains how antibiotic-resistance genes could first become associated with integrons.
authors.affiliation: Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
authors.forename: M V
authors.initials: MV
authors.lastname: Francia
authors.forename: F
authors.initials: F
authors.lastname: de la Cruz
authors.forename: J M
authors.initials: JM
authors.lastname: García Lobo
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D019426:Integrases; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases; D019426:Integrases