Characterization and strain identification of a potato virus Y isolate non-reactive with monoclonal antibodies specific to the ordinary and necrotic strains.
An isolate of potato virus Y, named PVY-36, reacted with polyclonal antibody against PVY-O (an ordinary strain), but not with any of eight monoclonal antibodies (MAbs) specific to PVY-O or with two MAbs specific to PVY-T (a necrotic strain). From its host range and symptomatology, PVY-36 belongs to the PVYO group. The nucleotide sequence of the coat protein (CP) coding region of the PVY-36 genome was determined and the amino acid sequence was predicted. Based on the CP amino acid sequence, PVY-36 is more closely related to PVY-O than to PVY-T. There were eight amino acid differences between the CPs of PVY-36 and PVY-O in the N-terminal 30 amino acids. It is suggested that amino acids 8-15 and/or 26-30 from the N-terminus may determine a PVY-O-specific epitope (or epitopes).
Hataya
T
T
Department of Agrobiology and Bioresources, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Inoue
A K
AK
Ohshima
K
K
Shikata
E
E
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D008969:Molecular Sequence Data; D017800:Potyvirus / Q000235:genetics* / Q000276:immunology* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000821:virology; D013045:Species Specificity; D015678:Viral Structural Proteins / Q000235:genetics
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015678:Viral Structural Proteins