Biochemical characterization of Bacillus thuringiensis cytolytic delta-endotoxins.
The entomocidal delta-endotoxins CytA and CytB produced by Bacillus thuringiensis (Bt) subspecies israelensis and kyushuensis respectively showed a similar level of toxicity to mosquito larvae but were not toxic to the larvae of the lepidopteran Manduca sexta. CytA and CytB are also similar in sequence, predicted secondary structure and alpha-helical content, the only obvious difference being a C-terminal fifteen residue 'tail' on CytB. Investigations of the function, if any, of the CytB C-terminal 'tail' showed that this delta-endotoxin is highly expressed and forms inclusions in an acrylstalliferous Bt mutant without the aid of the 20 kDa 'helper' protein from Bt subspecies israelensis which is essential for CytA inclusion formation. After proteinase K treatment, CytA and CytB were processed to virtually the same points in a sequence alignment and were equally haemolytic in vitro. However, the results suggested that unprocessed CytB differs from unprocessed CytA in that the former is not haemolytic.
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Department of Biochemistry, University of Cambridge, UK.
Ellar
D J
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D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry* / Q000502:physiology; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D001427:Bacterial Toxins; D001681:Biological Assay; D002942:Circular Dichroism; D009033:Culicidae / Q000187:drug effects / Q000254:growth & development; D004731:Endotoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D006461:Hemolysis; D007814:Larva; D018402:Manduca / Q000187:drug effects / Q000254:growth & development; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011817:Rabbits; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis