title: The influences of the buffer capacity of various substances on pH changes in dental plaque.
abstract: This study clarified the suitable pKa value for buffering substances against plaque pH fall in vitro and simultaneously estimated the effect of low molecular chitosan (LMCS) on plaque pH lowered by metabolized acids in vitro and in vivo. Five buffering substances with different pKa, aspartame (pKa: 7.8), phosphate buffer (7.1), LMCS (6.4), maleate buffer (6.2), and monofluorophosphate (4.8), were tested in this study. In the method using S. mutans cells, phosphate inhibited the pH fall from an initial pH of over 7.0, but phosphate exhibited no effect when the initial pH was 6.0. By the addition with lactic acid, LMCS and maleate exhibited more effective inhibition of the pH-fall than the others. These observations imply that pKa value is an important indicator of the ability of a buffering substance to reduce pH fall in dental plaque and that the optimum pKa value may be around pH 6.3. In the plaque pH measurement using ISFET electrode, LMCS showed an additional effect in inhibiting plaque pH fall following direct application of the glucose solution. The findings indicate that LMCS may be useful as a food additive to decrease the cariogenicity of foods.
authors.forename: K
authors.initials: K
authors.lastname: Shibasaki
authors.forename: H
authors.initials: H
authors.lastname: Sano
authors.forename: T
authors.initials: T
authors.lastname: Matsukubo
authors.forename: Y
authors.initials: Y
authors.lastname: Takaesu
mesh_terms: D000328:Adult; D001218:Aspartame / Q000494:pharmacology; D002021:Buffers; D002686:Chitin / Q000031:analogs & derivatives / Q000494:pharmacology; D048271:Chitosan; D003773:Dental Plaque / Q000737:chemistry*; D005459:Fluorides / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008297:Male; D008298:Maleates / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology
chemical_list: D002021:Buffers; D008298:Maleates; D010710:Phosphates; D002686:Chitin; C012980:fluorophosphate; D048271:Chitosan; D005459:Fluorides; D001218:Aspartame