Effects of preservation and sterilization on cortical bone grafts. A scanning electron microscopic study.
The effects of preservation and sterilization on the structural properties of cortical bone were investigated. Specimens of cortical bone from rat tibiae were frozen (-70 degrees C for 28 days), freeze-dried, irradiated (1, 5, 25 and 50 kGy) or autoclaved (at 134 degrees C for 3 or 5 min), and examined by scanning electron microscopy. Cryopreservation and irradiation had no deleterious effects on the surface structure of the cortical bone. Freeze-drying caused microcracks running parallel to the mineralized fiber bundles. After autoclaving, a time-dependent distension, swelling and amalgamation of the fibrillary matrix was observed. This denaturation of the organic matrix was more pronounced after 5 min than 3 min autoclaving. The alterations of the fibrillary structure described above might be due to a preservation- and sterilization-induced decrease of the biological and biomechanical potential of bone grafts.
Voggenreiter
G
G
Abteilung für Unfallchirurgie, Universität GHS Essen, Germany.
Ascherl
R
R
Blümel
G
G
Schmit-Neuerburg
K P
KP
D000818:Animals; D001842:Bone and Bones / Q000528:radiation effects* / Q000648:ultrastructure*; D015925:Cryopreservation; D008855:Microscopy, Electron, Scanning; D009926:Organ Preservation / Q000379:methods; D051381:Rats; D013242:Sterilization; D013977:Tibia / Q000648:ultrastructure