Interleukin-8 processing by neutrophil elastase, cathepsin G and proteinase-3.
Activated neutrophils secrete two forms of IL-8 with 77 and 72 amino acids, IL-8(77) and IL-8(72), along with proteinases that could process these cytokines. Significant conversion of IL-8(77) to more potent, N-terminally truncated forms was observed upon incubation with neutrophil granule lysates and purified proteinase-3. IL-8(72) was considerably more resistant to proteolytic processing than IL-8(77). The present observations indicate that neutrophil proteinases released in inflamed tissues convert IL-8 to more active forms and therefore tend to conserve or enhance, rather than decrease IL-8 activity.
Padrines
M
M
Theodor-Kocher Institute, University of Bern, Switzerland.
Wolf
M
M
Walz
A
A
Baggiolini
M
M
D000595:Amino Acid Sequence; D056649:Cathepsin G; D002403:Cathepsins / Q000378:metabolism*; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8 / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000235:genetics / Q000378:metabolism*; D019272:Leukocyte Elastase; D008969:Molecular Sequence Data; D053146:Myeloblastin; D018375:Neutrophil Activation; D009504:Neutrophils / Q000378:metabolism*; D010196:Pancreatic Elastase / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012697:Serine Endopeptidases / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism; D001620:beta-Thromboglobulin
C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D016326:Extracellular Matrix Proteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; C061615:PPBP protein, human; D010455:Peptides; D001620:beta-Thromboglobulin; C009862:connective tissue-activating peptide; D002403:Cathepsins; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C534094:CTSG protein, human; D056649:Cathepsin G; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D014357:Trypsin; D053146:Myeloblastin