Pulsating electromagnetic field stimulates mRNA expression of bone morphogenetic protein-2 and -4.
The effects of a pulsating electromagnetic field on mRNA expression of bone morphogenetic protein-2 and -4 in chick embryonic calvaria were examined. From the onset of embryogenesis (Day 0), chick embryos were incubated in a continuously generated pulsating electromagnetic field with a peak of 3.5 milli-Tesla (mean: 2 milli-Tesla) and vibration at 15 Hz. Control chicks were incubated in a normal magnetic field. Northern-blot analysis showed that the mRNAs of bone morphogenetic protein-2 and -4 were expressed in the calvaria. Quantitative analysis of the mRNA expressions was done by means of slot-blot hybridization. The magnetic field enhanced the expressions of both mRNAs. The enhancements were more pronounced in younger chick embryos (Day 15 > Day 17), and no significant change was observed in the 19-day-old embryos. These results indicate that osteo-inductive effects of the magnetic field were mediated at least in part by bone morphogenetic protein-2 and -4.
Nagai
M
M
Department of Biochemistry, Iwate Medical University School of Dentistry, Japan.
Ota
M
M
D000818:Animals; D015152:Blotting, Northern; D019485:Bone Morphogenetic Proteins; D002642:Chick Embryo; D004574:Electromagnetic Fields; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D006133:Growth Substances / Q000096:biosynthesis*; D015151:Immunoblotting; D010012:Osteogenesis / Q000528:radiation effects*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis
D019485:Bone Morphogenetic Proteins; D016326:Extracellular Matrix Proteins; D006133:Growth Substances; D012333:RNA, Messenger