Interactions between the Escherichia coli cyclic AMP receptor protein and RNA polymerase at class II promoters.
The effects of a number of mutations in crp have been measured at different cyclic AMP receptor protein (CRP)-dependent Class II promoters, where the CRP-binding site is centred around 41 1/2 base pairs upstream from the transcription start point. The amino acid substitutions HL159 and TA158 result in reduced CRP-dependent activation, but the reduction varies from one Class II promoter to another. Deletions in the C-terminus of the RNA polymerase alpha subunit suppress the effects of HL159 and TA158. The role of the C-terminus of alpha at these promoters is assessed. Other changes at E58, K52 and E96 affect CRP activity specifically at Class II promoters and their role is discussed.
West
D
D
School of Biochemistry, University of Birmingham, UK.
Williams
R
R
Rhodius
V
V
Bell
A
A
Sharma
N
N
Zou
C
C
Fujita
N
N
Ishihama
A
A
Busby
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002373:Cyclic AMP Receptor Protein / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D013489:Suppression, Genetic; D014158:Transcription, Genetic
D002373:Cyclic AMP Receptor Protein; D017931:DNA Primers; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases