title: Felbamate measured in serum by two methods: HPLC and capillary electrophoresis.
abstract: We have developed two methods for determining serum concentrations of felbamate, a new anticonvulsant drug. The first method is based on protein precipitation with acetonitrile, followed by HPLC. The between-run CV for this method is 5.7% (mean 55 mg/L), and the linearity extends from 5 to 175 mg/L. Results by this method compared well with those by an HPLC method based on chloroform extraction (r = 0.98, n = 21). In the second method, based on micellar electrokinetic capillary chromatography, the drug is measured by capillary electrophoresis with direct injection of serum. This method can be completed in 5 min compared with 12 min for the HPLC method, and there is no need for sample extraction. The between-run CV is 5.2% (mean 58 mg/L) and the linearity range is 5-160 mg/L. Results of this direct method correlated well (r = 0.98, n = 37) with those by the HPLC assay. The mean trough serum concentration of felbamate in 123 patients taking this drug was 44.9 mg/L (range 12-129 mg/L).
authors.affiliation: Department of Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157.
authors.forename: Z K
authors.initials: ZK
authors.lastname: Shihabi
authors.forename: K S
authors.initials: KS
authors.lastname: Oles
mesh_terms: D000097:Acetonitriles; D000927:Anticonvulsants / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism; D002197:Capillary Action; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods* / Q000706:statistics & numerical data; D004586:Electrophoresis / Q000191:economics / Q000379:methods* / Q000706:statistics & numerical data; D005188:False Negative Reactions; D000078328:Felbamate; D006801:Humans; D006863:Hydrogen-Ion Concentration; D048448:Phenylcarbamates; D011409:Propylene Glycols / Q000097:blood*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000097:Acetonitriles; D000927:Anticonvulsants; D001798:Blood Proteins; D048448:Phenylcarbamates; D011409:Propylene Glycols; D000078328:Felbamate; C032159:acetonitrile