Arachidonate lipoxygenase metabolite formation in gestational tissues.
Products of arachidonic acid metabolism via the lipoxygenase pathways have a potential role in the onset of human labor. We have determined whether activation of protein kinase C can modulate the rate of biosynthesis within the uterus of five important arachidonate lipoxygenase metabolites, i.e., leukotriene B4 (LTB4), LTC4, 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE and 15-HETE. Amnion, chorion laeve and decidual cells were isolated, grown to confluence and incubated with phorbol 12-myristate 13-acetate (PMA). PMA caused concentration-related stimulation of 5-HETE, 12-HETE and 15-HETE (but not LTB4) production; there were some stimulatory actions on decidual and amnion (but not chorion) LTC4 production. We postulate that activation of protein kinase C can result in enhanced production of arachidonate lipoxygenase metabolites that may have actions on the parturient process.
Edwin
S S
SS
Department of Obstetrics and Gynecology University of Utah School of Medicine, Salt Lake City 84132.
Mitchell
M D
MD
D000650:Amnion / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D001091:Arachidonate Lipoxygenases / Q000378:metabolism*; D002823:Chorion / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D003656:Decidua / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D005260:Female; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis* / Q000502:physiology; D007975:Leukotriene B4 / Q000096:biosynthesis* / Q000502:physiology; D017997:Leukotriene C4 / Q000096:biosynthesis* / Q000502:physiology; D011247:Pregnancy; D015398:Signal Transduction / Q000502:physiology
D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D017997:Leukotriene C4; D001091:Arachidonate Lipoxygenases