Effect of growth factors on bovine blastocyst development in a serum-free medium.
To investigate the effect of growth factors on pre-implantation development, bovine zygotes, produced by in vitro fertilization (IVF) of in vitro-matured (IVM) oocytes, were cultured in a serum-free medium to which the following growth factors were added one at a time: epidermal growth factor (EGF), acidic fibroblast growth factor (a-FGF), insulin-like growth factor-II (IGF-II), platelet-derived growth factor from human platelets (PDGF), and platelet-derived growth factor-AB, human, recombinant (PDGF-AB). All growth factors were added at a dose of either 10 or 50 ng/ml, except PDGF which was added at a dose of either 5 or 15 ng/ml. The control medium was TCM 199 supplemented with sodium pyruvate (0.25 mmol/l), BSA (10 mg/ml), insulin (5 micrograms/ml), transferrin (5 micrograms/ml), and sodium selenite (5 ng/ml). Embryos were cultured for 8 days (day of insemination = Day 0). The mean percentages of first cleavage on Day 2 varied from 67% to 86% and the differences between the 2 doses, or between the control and growth factor-treated groups were not significant (p > or = 0.13). The effects of the two doses on subsequent development up to the blastocyst stage did not differ either (p > or = 0.12). There was no stimulatory effect of any of the used exogenous growth factors on embryo development up to the morula or blastocyst stage on Day 7, or blastocyst stage on Day 8. Moreover, medium supplemented with PDGF had fewer blastocysts than the control (p < or = 0.03).(ABSTRACT TRUNCATED AT 250 WORDS)
Shamsuddin
M
M
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects*; D002417:Cattle / Q000196:embryology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans
D016895:Culture Media, Serum-Free; D006133:Growth Substances