Bimodal opioid regulation of cyclic AMP formation: implications for positive and negative coupling of opiate receptors to adenylyl cyclase.
A mu-selective opiate receptor agonist, sufentanil, can either increase or decrease the stimulated formation of cyclic AMP (cAMP) in the myenteric plexus. The direction of the opioid modulation of this second messenger depends on the concentration of opioid used. Low doses of opioid enhance, whereas higher concentrations inhibit, the magnitude of cAMP that is formed in response to electrical stimulation. Opioids exert this dual regulation on only stimulated cAMP formation. Basal levels are not affected. Opioid facilitation and inhibition of stimulated cAMP formation are blocked by naloxone, indicating mediation by opiate receptors. Because all experiments were conducted in the presence of a phosphodiesterase inhibitor, it is highly unlikely that opioid regulation of stimulated cAMP formation is due to changes in the rate of its degradation. Positive and negative coupling of mu-opiate receptors to adenylyl cyclase is the most plausible explanation for the bimodal opioid effects on cAMP content. The marked parallel between the current observations and the previously reported bimodal opioid regulation of evoked enkephalin release is consistent with the hypothesis that adenylyl cyclase is one biochemical substrate for the bimodal opiate receptor-coupled regulatory mechanism governing the stimulated release of this opioid peptide.
Wang
L
L
Department of Biochemistry, State University of New York, Health Science Center at Brooklyn 11203.
Gintzler
A R
AR
D000262:Adenylyl Cyclases / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004558:Electric Stimulation; D004745:Enkephalins / Q000378:metabolism; D006801:Humans; D009132:Muscles / Q000294:innervation; D009197:Myenteric Plexus / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D009270:Naloxone / Q000494:pharmacology; D009294:Narcotics / Q000494:pharmacology*; D011863:Radioimmunoassay; D011957:Receptors, Opioid / Q000032:analysis / Q000378:metabolism*; D017409:Sufentanil / Q000494:pharmacology
D004745:Enkephalins; D009294:Narcotics; D011957:Receptors, Opioid; D009270:Naloxone; D017409:Sufentanil; D000242:Cyclic AMP; D000262:Adenylyl Cyclases