title: Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C.
abstract: rab3A, a low molecular weight GTP-binding protein of synaptic vesicles with a putative function in synaptic vesicle docking, interacts in a GTP-dependent manner with rabphilin-3A, a peripheral membrane protein that binds Ca2+ and phospholipids. We now show that rabphilin-3A is an evolutionarily conserved synaptic vesicle protein that is attached to synaptic vesicle membranes via its N terminus and exhibits a heterogeneous distribution among synapses. In rab3A-deficient mice, rabphilin-3A is decreased in synapses belonging to neurons that primarily express rab3A and accumulates in the perikarya of these neurons. In contrast, neurons expressing significant levels of rab3C still contain normal levels of rabphilin-3A in a synaptic pattern, and rabphilin-3A binds rab3C in vitro. These results suggest that analogous to the membrane recruitment of raf by ras, rab3A and rab3C may function in recruiting rabphilin-3A to the synaptic vesicle membrane in a GTP-dependent manner.
authors.affiliation: Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Takei
authors.forename: M
authors.initials: M
authors.lastname: Geppert
authors.forename: L
authors.initials: L
authors.lastname: Daniell
authors.forename: K
authors.initials: K
authors.lastname: Stenius
authors.forename: E R
authors.initials: ER
authors.lastname: Chapman
authors.forename: R
authors.initials: R
authors.lastname: Jahn
authors.forename: P
authors.initials: P
authors.lastname: De Camilli
authors.forename: T C
authors.initials: TC
authors.lastname: Südhof
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D001923:Brain Chemistry; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins / Q000032:analysis / Q000172:deficiency / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D005982:Glutathione Transferase / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D009474:Neurons / Q000737:chemistry; D051381:Rats; D011993:Recombinant Fusion Proteins; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D020692:rab3 GTP-Binding Proteins; D000097624:Rabphilin-3A
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018076:DNA, Complementary; D019204:GTP-Binding Proteins; D005982:Glutathione Transferase; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D020692:rab3 GTP-Binding Proteins; D000097624:Rabphilin-3A