Fluorescence in situ hybridisation of multiple probes on a single microscope slide.
We report a method to analyse multiple samples by fluorescence in situ hybridisation on a single glass microscope slide. Wells were formed in which independent hybridisation reactions could proceed by sealing a silicon rubber gasket to the slide. In the largest format tested, different probes were hybridised simultaneously by applying them directly from a 96-well microtitre dish which was inverted on a glass plate. This technique will increase the rate of analysis of multiple probes against a standard set of chromosomes and could also be used to analyse different karyotypes using a panel of probes such as single chromosome paints during a single operation. It should be useful for both chromosomal mapping projects and screening for chromosome abnormalities in clinical diagnostic laboratories.
Larin
Z
Z
Department of Biochemistry, Oxford University, UK.
Fricker
M D
MD
Maher
E
E
Ishikawa-Brush
Y
Y
Southern
E M
EM
D002875:Chromosomes; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods*; D007621:Karyotyping / Q000379:methods
D015342:DNA Probes