A serum-free medium for human primary T lymphocyte culture.
This paper describes a defined serum-free medium (SFM), designated A-X, which supports in vitro proliferation of human primary T cells stimulated with mitogens, two-way mixed leukocyte reactions, anti-CD3, and a superantigen. A-X is a 1:1 mixture of two commercially available SFM, AIM V and EX-CELL 300. In each assay tested it supported uptake of [3H]thymidine as well as or better than the standard culture medium, namely RPMI 1640 supplemented with 10% fetal calf serum. A-X also allowed the detection of interleukin-2 by ELISA at levels comparable to those produced in serum-supplemented medium. A-X will likely be useful in further studies as it eliminates many of the problems usually associated with the use of serum-supplemented media.
Causey
A L
AL
Department of Pediatrics, University of Mississippi Medical Center, Jackson 39216-4505.
Wooten
R M
RM
Clem
L W
LW
Bly
J E
JE
D002478:Cells, Cultured; D003208:Concanavalin A / Q000276:immunology; D016895:Culture Media, Serum-Free / Q000737:chemistry*; D004768:Enterotoxins / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis; D008213:Lymphocyte Activation / Q000276:immunology; D010835:Phytohemagglutinins / Q000276:immunology; D011043:Pokeweed Mitogens / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*
D016895:Culture Media, Serum-Free; D004768:Enterotoxins; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A; C031818:enterotoxin B, staphylococcal