title: Genetic complementation of a floral homeotic mutation, apetala3, with an Arabidopsis thaliana gene homologous to DEFICIENS of Antirrhinum majus.
abstract: Among the homeotic mutants with altered floral organs, two mutants of Arabidopsis thaliana, apetala3 and pistillata, and two mutants of Antirrhinum majus, deficiens and globosa, have a homeotic conversion of the floral organs in whorl 2 and 3, namely petals to sepals and stamens to carpels. We have isolated a homologue of the DEFICIENS gene from A. thaliana wild type and shown complete complementation of apetala3 mutation by introducing the isolated gene using Agrobacterium-mediated transformation. These results show that the APETALA3 is a homologue of DEFICIENS structurally and functionally. The 5'-upstream region of APETALA3 contains three SRE-like sequence, where MADS box-containing proteins are assumed to bind and regulate expression in tissue- and stage-specific manner.
authors.affiliation: Division I of Gene Expression and Regulation, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okamoto
authors.forename: A
authors.initials: A
authors.lastname: Yano
authors.forename: H
authors.initials: H
authors.lastname: Shiraishi
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: Y
authors.initials: Y
authors.lastname: Shimura
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005801:Genes, Homeobox / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005816:Genetic Complementation Test; D015698:Genomic Library; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018547:Plant Stems / Q000737:chemistry; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C106162:HFI1 protein, S cerevisiae; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators