title: Promutagen activation by fish liver as a biomarker of littoral pollution.
abstract: Metabolic activation of known promutagens by liver S9 fractions of Mugil sp. (grey mullet) from two zones of the South Atlantic Spanish littoral was determined and related to their pollution levels. Sediments from the putative contaminated area contained high concentrations of PAHs, PCBs and pesticides, and animals from the polluted site exhibited higher concentrations of metals than those from the reference area. Hepatic S9 fractions of mullets from the polluted site showed 5.1-, 18.6- and 42.8-fold higher capability to activate benzo(a)pyrene, 2-acetyl-aminofluorene and 2-aminoanthracene, respectively, than those from reference animals. Cadmium, a highly toxic metal, was one of the pollutants detected in the contaminated area. Gilthead seabream (Sparus aurata) were exposed under controlled conditions to different Cd concentrations in order to investigate the effects of Cd on fish promutagen activation capability. A clear dose-response relationship was observed between Cd concentration, EROD activity and metabolic activation of 2-aminoanthracene and benzo(a)pyrene. Our data indicate that the enhanced promutagen activation by fish S9 fractions accompanying induction of EROD activity is a sensitive and reliable index of pollution in aquatic environments.
authors.affiliation: Departamento de Genética, Facultad de Ciencias, Universidad de Córdoba, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez-Ariza
authors.forename: G
authors.initials: G
authors.lastname: Dorado
authors.forename: J I
authors.initials: JI
authors.lastname: Navas
authors.forename: C
authors.initials: C
authors.lastname: Pueyo
authors.forename: J
authors.initials: J
authors.lastname: López-Barea
mesh_terms: D000818:Animals; D015415:Biomarkers; D001711:Biotransformation; D002104:Cadmium / Q000032:analysis / Q000493:pharmacokinetics; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009153:Mutagens / Q000032:analysis / Q000493:pharmacokinetics*; D010473:Perciformes; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000493:pharmacokinetics; D011083:Polycyclic Compounds / Q000032:analysis / Q000493:pharmacokinetics; D051381:Rats; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000493:pharmacokinetics*
chemical_list: D015415:Biomarkers; D009153:Mutagens; D011083:Polycyclic Compounds; D014874:Water Pollutants, Chemical; D002104:Cadmium; D011078:Polychlorinated Biphenyls