The two-hybrid system: an assay for protein-protein interactions.
The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. It can be used to identify proteins that bind to a protein of interest, or to delineate domains or residues critical for an interaction. Variations on this methodology have been developed to clone genes that encode DNA-binding proteins, to identify peptides that bind to a protein and, potentially, to screen for drugs.
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Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook 11794.
Sternglanz
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D004268:DNA-Binding Proteins / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
D004268:DNA-Binding Proteins; D011506:Proteins