The differentiation of parasitic nematodes using random amplified polymorphic DNA.
DNA from species and races of plant parasitic nematodes (Meloidogyne, Globodera and Heterodera) and a human parasitic nematode (Trichinella) were subjected to polymerase chain reaction amplification using one arbitrary primer (M-10). This technique results in relatively simple DNA profiles that include polymorphic markers known as random amplified polymorphic DNA (RAPDs). The RAPD profiles of the plant nematode species of Meloidogyne made possible the identification of M. incognita and M. hapla, but no differences were found between the patterns of M. javanica, M. arenaria and M. graminicola. Moreover, the four races of M. incognita were indistinguishable by this primer. In contrast, when races of the plant nematode Globodera rostochiensis (Ro1 and Ro2/3) were studied under the same RAPDs conditions, a race specific profile allows these two most devastating races to be differentiated. When DNAs of eight Trichinella isolates were subjected to RAPD studies, four different patterns were identified, corresponding to the four Trichinella clusters previously defined by isozyme polymorphism.
Chacón
M R
MR
Department of Biochemistry, Wellcome Centre for Parasitic Infections, Imperial College of Science, Technology and Medicine, London, UK.
Rodriguez
E
E
Parkhouse
R M
RM
Burrows
P R
PR
Garate
T
T
D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000737:chemistry; D018509:DNA, Helminth / Q000032:analysis* / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D009348:Nematoda / Q000145:classification* / Q000235:genetics; D010935:Plant Diseases / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D014234:Trichinella / Q000145:classification* / Q000235:genetics
D017931:DNA Primers; D018509:DNA, Helminth