Purification, expansion, and multiple fluorochrome labeling of cord blood hemopoietic precursors: preliminary results.
CD34-positive cells were isolated from a total of 23 cords using CellPro Ceprate columns. AIS MicroCellector flasks, and panning. The cells were (1) expanded in serum-free culture supplemented with a variety of combinations of cytokines and (2) immunophenotyped using multiple fluorochrome labeling. The results indicated that the avidin column produced the highest purity of CD34-positive cells, and that immature blast cells could be expanded in serum-free culture. Preliminary results suggested that the four fluorochrome labeling technique may provide useful information on the lineage commitment of cord blood precursor and blast cells.
Feron
C
C
Service of Biological, CHU du Sart-Tilman, Liège, Belgium.
Gothot
A
A
Grosdent
J C
JC
van Cauwenberge
J R
JR
Schaaps
J P
JP
Beguin
Y
Y
Paulus
J M
JM
D001360:Avidin; D001710:Biotin; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation / Q000295:instrumentation; D016895:Culture Media, Serum-Free; D016207:Cytokines / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology*; D005456:Fluorescent Dyes; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007163:Immunosorbent Techniques; D007231:Infant, Newborn
D016895:Culture Media, Serum-Free; D016207:Cytokines; D005456:Fluorescent Dyes; D001360:Avidin; D001710:Biotin