title: Sterility and the disinfection potential of Indian contact lens solutions.
abstract: Ocular infection associated with microbial contamination of contact lens care products is a major problem in contact lens wearers. The sterility and the antimicrobial activity of contact lens care systems reflect their suitability for disinfection of contact lenses. These factors remain to be evaluated for the various newer contact lens care products manufactured in India. In this study, 35 bottles of contact lens solutions marketed by different manufacturing units in India were tested for sterility. Seven solutions were tested for antimicrobial effectiveness employing the D value method of analysis. The D value is defined as the time required to reduce a population of organisms by 90% (one log unit). A standard inoculum of the ocular isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Serratia marcescens, Aspergillus fumigatus, Fusarium solani, and Acanthamoeba castellanii were used as challenge organisms. Bacterial contamination was detected in 20 (57.1%) solution bottles and none yielded fungus or Acanthamoeba. Pseudomonas species were the most commonly encountered contaminant (11/20; 55%). Only sterile solutions were analyzed for antimicrobial activity. D values ranging between 12 and 20 minutes were demonstrated by six of the seven solutions against bacterial challenge. Good antifungal activity was noticed in five solutions against Fusarium solani though results varied with Aspergillus flavus and Candida albicans. All solutions were adequately effective against Acanthamoeba.
authors.affiliation: Sri Devchand Nagardas Jhaveri Microbiology Centre, Hyderabad.
authors.forename: U
authors.initials: U
authors.lastname: Gopinathan
authors.forename: S
authors.initials: S
authors.lastname: Sharma
authors.forename: S
authors.initials: S
authors.lastname: Boghani
authors.forename: G N
authors.initials: GN
authors.lastname: Rao
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D018639:Contact Lens Solutions / Q000494:pharmacology; D004340:Drug Contamination; D056890:Eukaryota / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D007194:India; D008826:Microbial Sensitivity Tests
chemical_list: D018639:Contact Lens Solutions