Transcriptional activation of the alpha-1 acid glycoprotein gene by YY1 is mediated by its functional interaction with a negative transcription factor.
Regulation of alpha-1 acid glycoprotein (AGP) gene expression involves both positive and negative transcription factors. We have previously identified two dominant factors: positive and negative transcription factors, AGP/EBP and factor B, respectively, involved in the transcription of AGP and other acute-phase response genes. In this report, we present evidence showing that the transcription of the AGP gene is positively regulated by a transcription factor, YY1. The activation of AGP gene by YY1 is mediated by a negative element B in the AGP promoter region. YY1 can also activate the B motif linked to a heterologous promoter. However, YY1 does not bind directly to the B motif per se. Rather, our data suggest that the activation of AGP gene by YY1 may be mediated by its functional interaction with factor B, which recognizes the B motif.
Lee
Y M
YM
Institute of Biological Chemistry, Academia Sinca, Taipei, Taiwan.
Lee
S C
SC
D000210:Acute-Phase Reaction / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000378:metabolism*; D050981:Erythroid-Specific DNA-Binding Factors; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009961:Orosomucoid / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D050986:YY1 Transcription Factor
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D009838:Oligodeoxyribonucleotides; D009961:Orosomucoid; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494700:Yy1 protein, rat