Molecular evolution of interleukin-3.
Chimpanzee, tamarin, and marmoset interleukin-3 (IL-3) genes were cloned, sequenced, and expressed. Western blot analysis demonstrated that functional genes were isolated. IL-3 sequences were compared with those of mouse, rat, rhesus monkey, gibbon, and man. Multiple alignment of the IL-3 coding regions showed that only a few regions had been conserved during mammalian evolution, which are likely associated with functional domains of the IL-3 protein. Substitution rates for the various lineages were calculated and the numbers of synonymous and nonsynonymous substitutions were estimated separately. Distance matrices of the IL-3 coding regions were used to construct phylogenetic trees which revealed large differences in IL-3 evolution rate as well as a more rapid substitution rate for rodents and a rate slowdown during hominoid evolution. Extremes were rhesus monkey IL-3, which accumulated few synonymous substitutions, and gibbon IL-3, which had almost exclusively synonymous substitutions. In rhesus monkey IL-3, nonsynonymous substitutions outnumbered synonymous substitutions, which could not be readily explained by a random process of substitutions. We assume that during evolution of IL-3, the majority of the amino acid replacements and the impaired interspecies functional cross-reactivity originate from selection mechanisms with the most likely selective force being the structure of the heterodimeric IL.3 cell-surface receptor. Insight into IL-3 architecture and structural analysis of the IL-3 receptor are needed to analyze the unusually fast evolution of IL-3 in more detail.
Burger
H
H
Department of Medical Oncology, Dr. Daniel den Hoed Cancer Center/Dijkzigt, University Hospital Rotterdam, The Netherlands.
Wagemaker
G
G
Leunissen
J A
JA
Dorssers
L C
LC
D000818:Animals; D005075:Biological Evolution; D002144:Callithrix / Q000235:genetics; D005091:Exons / Q000235:genetics; D006801:Humans; D006922:Hylobates / Q000235:genetics; D007377:Interleukin-3 / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008253:Macaca mulatta / Q000235:genetics; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000235:genetics; D010802:Phylogeny; D051381:Rats / Q000235:genetics; D012452:Saguinus / Q000235:genetics; D016415:Sequence Alignment
D007377:Interleukin-3