A novel experimental model for the study and evaluation of experimental vaccines to Actinobacillus pleuropneumoniae.
A novel experimental model to study immune protection to Actinobacillus pleuropneumoniae in the rat is described. One-week-old rats born from immunized mothers were challenged intraperitoneally with a suspension of A. pleuropneumoniae serotype 1 and mortality recorded up to 48 h postinfection. Immunization with inactivated whole cells (IWC) or a whole cell extract (WCE) from A. pleuropneumoniae serotype 1 resulted in protection against an homologous challenge, particularly in the case of WCE where protection was observed at the highest challenge dose of approximately 1000 50% lethal doses.
Montaraz
J A
JA
Facultad de Estudios Superiores-Cuautitlán (UNAM), Mexico.
Rosales
M E
ME
Bautista
E
E
Barcenas
G
G
Lara
V
V
D000189:Actinobacillus Infections / Q000401:mortality / Q000517:prevention & control*; D016977:Actinobacillus pleuropneumoniae / Q000276:immunology*; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D002457:Cell Extracts / Q000008:administration & dosage; D005260:Female; D008954:Models, Biological; D051381:Rats; D017208:Rats, Wistar; D015164:Vaccines, Inactivated / Q000008:administration & dosage
D001428:Bacterial Vaccines; D002457:Cell Extracts; D015164:Vaccines, Inactivated