Adenoviral vectors as functional retrograde neuronal tracers.
Adenoviruses have been recently recognized as a highly efficient system for gene delivery to various tissues. The ability of replication-defective recombinant adenovirus to transfer the lacZ reporter gene encoding beta-galactosidase to nerve cells in various brain structures has been demonstrated. Here, on the continuation of these studies, we present evidence that the adenovirus can be transported in a retrograde manner to nerve cell bodies from axonal terminals. This method may be of great value for infecting selected subsets of specific neurons for either anatomo-functional studies or even therapeutic purposes.
Ridoux
V
V
Institut Alfred Fessard, Centre National de la Recherche Scientifique [CNRS], Gif sur Yvette, France.
Robert
J J
JJ
Zhang
X
X
Perricaudet
M
M
Mallet
J
J
Le Gal La Salle
G
G
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology; D005822:Genetic Vectors; D008297:Male; D017072:Neostriatum / Q000201:enzymology / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012091:Repetitive Sequences, Nucleic Acid; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D014446:Tyrosine 3-Monooxygenase; D001616:beta-Galactosidase