Suppression of human T-cell growth in vitro by cis-unsaturated fatty acids: relationship to free radicals and lipid peroxidation.
Cis-unsaturated fatty acids such as dihomogamma-linolenic acid (DGLA), arachidonic acid (AA) and eicosapentaenoic acid (EPA), which form precursors to 1, 2 and 3 series prostaglandins (PGs), have been shown to suppress human T-cell growth in vitro by a prostaglandin E (PGE)-independent mechanism. In an earlier study, we showed that these fatty acids can induce free radical generation in human neutrophils and tumor cells. Here we show that cis-unsaturated fatty acids augment free radical generation and lipid peroxidation in human T-cells. The growth suppressive action of cis-unsaturated fatty acids on human T-cells could be blocked by anti-oxidant, vitamin E and the superoxide anion quencher superoxide dismutase. These results suggest that c-UFAs-induced cell growth suppression is a free radical dependent process.
Madhavi
N
N
Department of Medicine, Nizam's Institute of Medical Sciences, Hyderabad, India.
Das
U N
UN
Prabha
P S
PS
Kumar
G S
GS
Koratkar
R
R
Sagar
P S
PS
D015126:8,11,14-Eicosatrienoic Acid / Q000494:pharmacology; D016718:Arachidonic Acid / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D015232:Dinoprostone / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008213:Lymphocyte Activation / Q000187:drug effects; D013482:Superoxide Dismutase / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology
D005231:Fatty Acids, Unsaturated; D005609:Free Radicals; D014810:Vitamin E; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D013482:Superoxide Dismutase; D015126:8,11,14-Eicosatrienoic Acid; D015232:Dinoprostone