title: Induction of the conversion of xanthine dehydrogenase to oxidase in rabbit liver by Cu2+,Zn2+ and selenium ions.
abstract: Effects of Cu2+,Zn2+,Fe2+ and selenium ions on the conversion of xanthine dehydrogenase to oxidase in rabbit liver were examined. Under basal conditions, xanthine oxidase activity represented only 16% of the total xanthine oxidase plus dehydrogenase activity. Cu2+ (2-10 microM), Zn2+ (5-30 microM) and selenium ions (5-100 microM) brought about the conversion of xanthine dehydrogenase to oxidase in a dose-dependent manner. The concentrations of Cu2+,Zn2+ and selenium ions required for increasing xanthine oxidase activity by 50% was approximately 4, 10 and 20 microM, respectively. On the other hand, Fe2+ had no effect on the conversion of the enzyme up to 100 microM. These results suggest that Cu2+,Zn2+ and selenium ions have the potential to modulate the conversion of xanthine dehydrogenase to oxidase in rabbit liver.
authors.affiliation: Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sakuma
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: M
authors.initials: M
authors.lastname: Iwai
authors.forename: K
authors.initials: K
authors.lastname: Fujimoto
authors.forename: M
authors.initials: M
authors.lastname: Yasuda
authors.forename: H
authors.initials: H
authors.lastname: Nishida
authors.forename: T
authors.initials: T
authors.lastname: Fujita
mesh_terms: D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000494:pharmacology*; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D011817:Rabbits; D012643:Selenium / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology; D014527:Uric Acid / Q000032:analysis; D014968:Xanthine Dehydrogenase / Q000187:drug effects / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000187:drug effects / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology*
chemical_list: D014527:Uric Acid; D000493:Allopurinol; D003300:Copper; D007501:Iron; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase; D014357:Trypsin; D012643:Selenium; D015032:Zinc