New restriction endonuclease CviRI cleaves DNA at TG/CA sequences.
A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5'-TGCA-3' and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5'-TGCA-3' sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.
Jin
A
A
Megabase Research Products, Lincoln, NE 68503.
Zhang
Y
Y
Xia
Y
Y
Traylor
E
E
Nelson
M
M
Van Etten
J L
JL
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002708:Chlorella / Q000201:enzymology / Q000821:virology; D003596:Cytosine; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006147:Guanine; D006801:Humans; D008745:Methylation; D013379:Substrate Specificity
D006147:Guanine; D003596:Cytosine; D004247:DNA; C089927:endodeoxyribonuclease CviRI; D015252:Deoxyribonucleases, Type II Site-Specific