Secondary-sites for integration mediated by the Tn21 integrase.
The integrase encoded by the integron of the transposon Tn21 can mediate the site-specific fusion of two plasmids if there is a recombination hot spot (59 bp element) in one of them and the sequence GWTMW in the other. The use of this latter, loosely defined site explains how antibiotic-resistance genes could first become associated with integrons.
Francia
M V
MV
Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
de la Cruz
F
F
García Lobo
J M
JM
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D019426:Integrases; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases; D019426:Integrases