CCD camera imaging for the chemiluminescent detection of enzymes using new ultrasensitive reagents.
We have designed and constructed an inexpensive imaging system based on charge-coupled device (CCD) technology and utilized it to demonstrate the sensitivity and rapid detection possible with Lumigen chemiluminescent reagents. We also report the development of two new chemiluminescent reagents, Lumi-Phos Plus and Lumigen PS. Lumi-Phos Plus is an enhanced formulation for the rapid detection of alkaline phosphatase on membranes and in solution. It provides excellent images in blotting applications with exposures of under a minute. Lumigen PS represents a new generation of peroxidase detection reagents. The wide dynamic range with excellent linearity, higher signal and lower background than other chemiluminescent reagents make Lumigen PS of unsurpassed value in enzyme-linked immunoassays and nucleic acid probe assays using HRP conjugates.
Akhavan-Tafti
H
H
Lumigen, Inc., Southfield, MI 48034.
Schaap
A P
AP
Arghavani
Z
Z
DeSilva
R
R
Eickholt
R A
RA
Handley
R S
RS
Schoenfelner
B A
BA
Sugioka
K
K
Sugioka
Y
Y
D000469:Alkaline Phosphatase / Q000032:analysis; D015139:Blotting, Southern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D004247:DNA / Q000032:analysis*; D004798:Enzymes / Q000032:analysis*; D006735:Horseradish Peroxidase / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D010781:Photography / Q000379:methods*; D010783:Photometry / Q000379:methods; D012680:Sensitivity and Specificity
D004798:Enzymes; D007202:Indicators and Reagents; D004247:DNA; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase