Identification of the ron gene product as the receptor for the human macrophage stimulating protein.
Macrophage-stimulating protein (MSP) is a member of the hepatocyte growth factor-scatter factor (HGF-SF) family. Labeled MSP bound to Madin-Darby canine kidney (MDCK) cells transfected with complementary DNA encoding Ron, a cell membrane protein tyrosine kinase. Cross-linking of 125I-labeled MSP to transfected cells (MDCK-RE7 cells) and immunoprecipitation by antibodies to Ron revealed a 220-kilodalton complex, a size consistent with that of MSP (80 kilodaltons) cross-linked to the beta chain of Ron (150 kilodaltons). The binding of 125I-labeled MSP to MDCK-RE7 cells was inhibited by unlabeled MSP, but not by HGF-SF. MSP caused phosphorylation of the beta chain of Ron and induced migration of MDCK-RE7 cells. These results establish the ron gene product as a specific cell-surface receptor for MSP.
Wang
M H
MH
Immunopathology Section, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702.
Ronsin
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C
Gesnel
M C
MC
Coupey
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L
Skeel
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A
Leonard
E J
EJ
Breathnach
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R
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D003432:Cross-Linking Reagents; D004285:Dogs; D006133:Growth Substances / Q000378:metabolism* / Q000494:pharmacology; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation; D010958:Plasminogen / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D003432:Cross-Linking Reagents; D006133:Growth Substances; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; C082332:macrophage stimulating protein; D017228:Hepatocyte Growth Factor; D010958:Plasminogen; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases