[Restriction fragment length polymorphism of Bam HI and Rsa I loci in the retinoblastoma gene].
The retinoblastoma (Rb) gene probe p123 M1.8 and p68 Rs2.0 were used to study the frequencies of the Bam HI and Rsa I restriction fragment length polymorphisms (RFLPs) in the RB gene among the population of Han nationality in Guangdong province. The result showed that the heterozygote rate of the Rsa I locus was only 55.0%, remarkably different from reports in the literature. Linkage study of the Bam HI and the Rsa I RFLPs demonstrated that 10.5% of the Rsa I polymorphic loci were heterozygous for the Bam HI RFLP. Thus the combined use of the two RFLPs can give information for about 65.5% of the population.
Wei
J
J
Sun Yat-sen University of Medical Sciences, Guangzhou.
Du
C S
CS
D044466:Asian People; D002681:China; D005260:Female; D005787:Gene Frequency; D016161:Genes, Retinoblastoma; D008040:Genetic Linkage; D006579:Heterozygote; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length