Immunological recognition of fibronectin-binding proteins of Staphylococcus aureus and Staphylococcus capitis, strain LK 499.
Antibodies to fibronectin-binding proteins (FnBPs) of Staphylococcus aureus, including binding domain of FnBPA, the D region, or the A-C regions of FnBPB were produced in rabbits and mice. These antibodies were used to characterize cell-associated FnBPs of S. aureus strain Cowan I, S. aureus strain U320 and a coagulase-negative Staphylococcus capitis strain LK499 as well as extracellular FnBPs in culture supernatants of the strain U320. FnBPs of S. aureus were predominantly FnBPA, while FnBPB was hardly detected on the cells or in culture supernatant of these S. aureus strains. Moreover, S. capitis strain LK499 possessed different FnBP(s) compared to S. aureus because the antibodies to S. aureus FnBPs did not recognize FnBP(s) on S. capitis.
Sakata
N
N
Department of Medical Microbiology, University of Lund, Sweden.
Rozalska
B
B
Wadström
T
T
D018829:Adhesins, Bacterial; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003429:Cross Reactions / Q000276:immunology; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D051379:Mice; D011485:Protein Binding; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013211:Staphylococcus aureus / Q000145:classification / Q000276:immunology*
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005353:Fibronectins; D011993:Recombinant Fusion Proteins; C078635:fibronectin-binding proteins, bacterial