Quantitation of erythrocyte-bound IgG by competitive solid-phase enzyme immunoassay.
A competitive solid-phase enzyme immunoassay technique for the quantitation of erythrocyte (RBC)-bound IgG was devised. This method is highly sensitive, reproducible and can be performed more simply. Anemic dogs with a positive Coombs' test result were observed to have higher RBC-bound IgG values (IgG concentration, ng/ml) than healthy dogs.
Adachi
K
K
Departments of Veterinary Internal Medicine, Miyazaki University, Japan.
Horii
Y
Y
Nagatomo
H
H
Shimizu
T
T
Makimura
S
S
D000740:Anemia / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D001404:Babesiosis / Q000097:blood / Q000276:immunology; D003298:Coombs Test / Q000662:veterinary; D004283:Dog Diseases; D004285:Dogs; D004912:Erythrocytes / Q000276:immunology*; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood*; D007962:Leukocytes / Q000502:physiology; D012016:Reference Values; D012680:Sensitivity and Specificity
D007074:Immunoglobulin G