Inhibition of lipid biosynthesis induces the expression of the pspA gene.
Treatment of Escherichia coli with diazaborine strongly induces the synthesis of a 28 kDa protein which is associated with the cytoplasmic membrane. The partial amino acid sequence proved that this protein is identical to the phage shock protein PspA. The kinetics of the expression of the pspA gene were determined in an E. coli strain which carried a pspA-lacZ fusion in the chromosome. PspA synthesis is independent of the growth phase. It is, however, strongly induced when fatty acid biosynthesis is inhibited by diazaborine or cerulenin. Treatment with either compound also causes dose-dependent inhibition of phospholipid biosynthesis whose degree correlates with the induction of PspA. Another cause of induction of PspA synthesis is treatment of E. coli with globomycin, which is an inhibitor of the processing of lipoproteins.
Bergler
H
H
Institut für Mikrobiologie, Karl-Franzens-Universität, Graz, Austria.
Abraham
D
D
Aschauer
H
H
Turnowsky
F
F
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D001896:Boron Compounds / Q000494:pharmacology*; D002569:Cerulenin / Q000494:pharmacology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009268:Nalidixic Acid / Q000494:pharmacology; D009285:Naphthoquinones / Q000494:pharmacology; D010455:Peptides; D010743:Phospholipids / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001896:Boron Compounds; D006360:Heat-Shock Proteins; D008055:Lipids; D008565:Membrane Proteins; D009285:Naphthoquinones; D010455:Peptides; D010743:Phospholipids; D011993:Recombinant Fusion Proteins; C069816:phage shock protein, Bacteria; D002569:Cerulenin; D009268:Nalidixic Acid; C017339:globomycin; C032255:1,2-dihydro-1-hydroxy-6-methyl-2-(propanesulfonyl)-thieno(3,2D)(1,2,3)-diazaborine; D006861:Hydrogen Peroxide