Isolation of a novel subgroup B feline leukemia virus from a cat infected with FeLV-A.
Proviruses were cloned directly from a cat that developed neurological disorders approximately 28 months after inoculation with a molecularly cloned, minimally pathogenic subgroup A feline leukemia virus (FeLV-A). In addition to FeLV-A proviruses that were nearly identical to the inoculated virus, we detected a subgroup B-like variant in brain, bone marrow, and lymph node that apparently had acquired the major portion of its extracellular envelope gene (gp70) from endogenous FeLV-related sequences. A similar recombinant was also detected, by PCR, at low levels in bone marrow from an early time postinfection (2.5 months). A full-length proviral variant with this recombinant structure cloned from brain tissue encoded a replication-defective virus. A chimera encoding the 5' gag-pol portion of FeLV-A and the 3' env-LTR portion of the defective brain-derived clone was replication-competent and had the extended host range properties of FeLV-B.
Boomer
S
S
Department of Microbiology, University of Washington, Seattle 98195.
Gasper
P
P
Whalen
L R
LR
Overbaugh
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D004279:DNA, Viral / Q000032:analysis; D005256:Leukemia Virus, Feline / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012192:Retroviridae Infections / Q000821:virology*; D017385:Sequence Homology; D014412:Tumor Virus Infections / Q000821:virology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
D004279:DNA, Viral; D014759:Viral Envelope Proteins