title: Calsequestrin is a major binding protein of myotoxin alpha and an endogenous Ca2+ releaser in sarcoplasmic reticulum.
abstract: Myotoxin alpha from prairie rattlesnakes induced Ca2+ release from the heavy fraction of sarcoplasmic reticulum at submicromolar concentrations. 125I-Labeled myotoxin alpha (125I-myotoxin alpha) specifically bound to the heavy fraction. Fractionation of the solubilized heavy fraction with a spermine-agarose column gave purified calsequestrin as a major binding protein of 125I-myotoxin alpha. We have first indicated that calsequestrin is a target protein for Ca(2+)-releasing action of myotoxin alpha. Calsequestrin probably plays a key role in physiological Ca2+ release from sarcoplasmic reticulum.
authors.affiliation: Department of Pharmaceutical Molecular Biology, Tohoku University, Sendai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohkura
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
authors.forename: A T
authors.initials: AT
authors.lastname: Tu
authors.forename: Y
authors.initials: Y
authors.lastname: Ohizumi
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002155:Calsequestrin / Q000378:metabolism*; D003435:Crotalid Venoms / Q000378:metabolism*; D017839:Crotalus; D011485:Protein Binding; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
chemical_list: D002155:Calsequestrin; D003435:Crotalid Venoms; C018932:myotoxin A; D002118:Calcium