title: Characterization of the principal colouring components of annatto using high performance liquid chromatography with photodiode-array detection.
abstract: The principal colouring components of the natural food colouring material annatto, the 9'-cis- and all-trans- isomers of bixin and norbixin, have been prepared in pure form. A reverse-phase HPLC method utilizing photodiode-array detection has been developed to enable their chromatographic and spectroscopic characterization. One minor component, a di-cis-isomer of norbixin has also been identified and characterized.
authors.affiliation: Ministry of Agriculture, Fisheries and Food, Food Science Laboratory, Colney, Norwich, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Scotter
authors.forename: S A
authors.initials: SA
authors.lastname: Thorpe
authors.forename: S L
authors.initials: SL
authors.lastname: Reynolds
authors.forename: L A
authors.initials: LA
authors.lastname: Wilson
authors.forename: P R
authors.initials: PR
authors.lastname: Strutt
mesh_terms: D002338:Carotenoids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005505:Food Coloring Agents / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D005505:Food Coloring Agents; D010936:Plant Extracts; D002338:Carotenoids