Two genomic variations in the E1 region of canine adenovirus type 2 strains.
We amplified the E1 region of canine adenovirus type 2 genomes by the polymerase chain reaction (PCR) and analyzed the PCR products by using eight restriction endonucleases. Restriction patterns of the E1 region cleaved with HaeIII and RsaI revealed two genomic variations among the canine adenovirus type 2 strains. Although the clinical significance of two distinct genotypes among the canine adenovirus type 2 strains is currently unknown, these genomic variations are well conserved among different strains in each genotype and suggest that the Japanese field strains, with reference to the E1 region, are different from the non-Japanese strains examined.
Harasawa
R
R
Animal Center for Biomedical Research, Faculty of Medicine, University of Tokyo, Japan.
Tohya
Y
Y
Yoshida
M
M
Kataoka
Y
Y
Katae
H
H
D006516:Adenoviruses, Canine / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000032:analysis; D004285:Dogs; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004262:DNA Restriction Enzymes