[Parasitic circulating antigens in hosts with Filaria bancrofti].
The ELISA and Dot-ELISA with rabbit anti-Setaria labial-papillosa antibody were applied to determine circulating antigens of Filaria bancrofti. The total positive rate was 96% (24/25) in the sera of patients with microfilaremia from endemic areas, Anhui province. The total positive rate of urines was 60% (15/25) in the same patients. The positive rates of the milks were both 100% (4/4) in the same patients. The conformity rate was 100% and 75% respectively for positive sera and urines (15 cases), milks (4). Negative reactions were observed in the sera, urines and milks of all the 43 healthy people from non-endemic areas in Jiangsu province. Negative reactions were observed in the urines of all the 11 non-filaria cases harbouring intestinal nematodes. The positive rate detected with ELISA was 36% (4/11) in the patient sera that were negative when they were detected by Dot-ELISA. Negative reactions were detected with Dot-ELISA in the sera of all the 10 non-filaria patients infected with Paragonimus westermani. The results showed that circulating antigens of Filaria bancrofti exist not only in the serum but also in the urine and the milk.
Yang
W
W
Department of Parasitology, Medical College of Yangzhou University.
Shen
Y
Y
Zhang
Z
Z
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood* / Q000652:urine; D004605:Elephantiasis, Filarial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008842:Microfilariae / Q000276:immunology; D008895:Milk, Human / Q000276:immunology; D014958:Wuchereria bancrofti / Q000276:immunology*
D000947:Antigens, Helminth