Mutations in the fusion peptide and heptad repeat regions of the Newcastle disease virus fusion protein block fusion.
Nonconservative mutations were introduced by site-specific mutagenesis into the fusion peptide and the adjacent heptad repeat region of the fusion protein of Newcastle disease virus in order to determine the role of both regions in the fusion activity of the protein. Mutations in both regions that allowed for proper folding and intracellular transport of the protein blocked the fusion activity of the protein when assayed in the presence of the hemagglutinin-neuraminidase protein.
Sergel-Germano
T
T
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.
McQuain
C
C
Morrison
T
T
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009522:Newcastle disease virus / Q000502:physiology*; D013329:Structure-Activity Relationship; D014760:Viral Fusion Proteins / Q000032:analysis / Q000737:chemistry / Q000502:physiology*
D014760:Viral Fusion Proteins