title: Tritium isotope effect in high-performance liquid chromatography of eicosanoids.
abstract: A significant difference in retention time between unlabeled and the corresponding multi-tritium-labeled eicosanoid has been observed in the high performance liquid chromatography (HPLC) analysis of 11 eicosanoids. Variations in retention time range from 3-7%, depending on the separation conditions as well as the number and position of the tritium substitution. Multi-tritium-labeled eicosanoids were eluted earlier than the corresponding unlabeled eicosanoid in reversed phase HPLC, whereas no isotope effect was seen with 14C- and 3H2-eicosanoids. Considerations must be given to this tritium isotope effect whenever both multi-tritium-labeled and unlabeled eicosanoids are used for HPLC cochromatography or recovery studies.
authors.affiliation: Lipids Group, DuPont, NEN Products, Boston, MA 02118.
authors.forename: U H
authors.initials: UH
authors.lastname: Do
authors.forename: S L
authors.initials: SL
authors.lastname: Lo
authors.forename: J
authors.initials: J
authors.lastname: Iles
authors.forename: T
authors.initials: T
authors.lastname: Rosenberger
authors.forename: P
authors.initials: P
authors.lastname: Tam
authors.forename: Y
authors.initials: Y
authors.lastname: Hong
authors.forename: D
authors.initials: D
authors.lastname: Ahern
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015777:Eicosanoids / Q000032:analysis*; D006859:Hydrogen; D015394:Molecular Structure; D014316:Tritium / Q000737:chemistry
chemical_list: D015777:Eicosanoids; D014316:Tritium; D006859:Hydrogen