First and second derivative spectrophotometric determination of cefoperazone and sulbactam in injections.
A simple, spectrophotometric assay to measure the concentrations of cefoperazone and sulbactam in injectable formulations is described. Since zero-order spectra are subject to interference, derivative spectrophotometry was used to enhance the spectral details. A linear relationship between derivative amplitudes and the concentrations of the compounds was found. Beer's law is obeyed up to 75 and 80 micrograms ml-1 of cefoperazone in the first and second derivative modes, respectively, and up to 75 micrograms ml-1 of sulbactam in the second derivative mode. Detection limits were 0.64 and 0.88 microgram ml-1, respectively for cefoperazone in the first and second derivative modes and 0.30 micrograms ml-1 for sulbactam in the second derivative mode. The method is rapid, simple, does not require a separation step and has successfully been applied to the assay of commercial injections containing cefoperazone and sulbactam.
Parra
A
A
Servicio de Farmacia, Hospital Virgen del Castillo, Murcia, Spain.
Garcia-Villanova
J
J
Rodenas
V
V
Gomez
M D
MD
D000892:Anti-Infective Agents, Urinary / Q000737:chemistry* / Q000378:metabolism; D002438:Cefoperazone / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D004304:Dosage Forms; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet; D013407:Sulbactam / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism
D000892:Anti-Infective Agents, Urinary; D004304:Dosage Forms; D004338:Drug Combinations; D002438:Cefoperazone; C057923:sulperazone; D013407:Sulbactam