Sequence-specific impairment of learning by c-jun antisense oligonucleotides.
Hybridization studies revealed a differential accumulation of c-jun and jun B mRNA in the hippocampus and cerebral cortex of rats trained on a foot-shock-motivated brightness discrimination. Supposing that early gene expression is functionally significant for plastic changes in the brain, we used antisense phosphorothioate oligodeoxynucleotides (S-ODN) in vivo to study effects of specific inhibition of c-jun and jun B gene expression on learning and memory formation in rat brain. Discrimination performance of rats was impaired after intrahippocampal injection of anti-c-jun S-ODN but not of anti-jun B S-ODN. These results imply that topically injected antisense oligonucleotides affect processes involved in learning and memory in a sequence-specific manner.
Tischmeyer
W
W
Federal Institute for Neurobiology, Magdeburg, Germany.
Grimm
R
R
Schicknick
H
H
Brysch
W
W
Schlingensiepen
K H
KH
D000818:Animals; D001483:Base Sequence; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D004192:Discrimination, Psychological; D004597:Electroshock; D015870:Gene Expression / Q000187:drug effects*; D016758:Genes, jun / Q000187:drug effects / Q000502:physiology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007858:Learning / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008568:Memory / Q000187:drug effects / Q000502:physiology; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D014796:Visual Perception / Q000187:drug effects
D016376:Oligonucleotides, Antisense