Expression cloning in K+ transport defective yeast and distribution of HBP1, a new putative HMG transcriptional regulator.
The rat HBP1 cDNA was cloned by its capacity to suppress the potassium transport-defective phenotype of mutant Saccharomyces cerevisiae cells. HBP1 cDNA encodes a 513 amino acids protein which, unexpectedly, does not share any homology with K+ transporters or K+ channels. However, a search in protein databases reveals that HBP1 contains a putative DNA-binding domain called HMG-box. Northern blot analysis shows that HBP1 is expressed in a variety of tissues and that in adipocyte and myogenic cell lines, its expression is directly related to differentiation. Taken together, the results suggest that the rat HBP1 is a new member of the HMG class of transcriptional regulators involved in cell differentiation pathways.
Lesage
F
F
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UPR411, Valbonne, France.
Hugnot
J P
JP
Amri
E Z
EZ
Grimaldi
P
P
Barhanin
J
J
Lazdunski
M
M
D017667:Adipocytes / Q000166:cytology; D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D015723:Gene Library; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins / Q000235:genetics* / Q000502:physiology; D017136:Ion Transport; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology; D009928:Organ Specificity; D011188:Potassium / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology
D018076:DNA, Complementary; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D006609:High Mobility Group Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D011188:Potassium