title: Intestinal lipid inhibits gastric emptying via CCK and a vagal capsaicin-sensitive afferent pathway in rats.
abstract: The mechanism by which lipid in the duodenum inhibits gastric emptying was investigated in awake rats fitted with chronic gastric and duodenal cannulas. Perfusion of the duodenum with lipid (Intralipid, 5 and 10%; total amount 50 and 100 mg) caused a significant inhibition (26 and 78%, respectively) of gastric emptying of a nonnutrient liquid (0.9% saline). Functional ablation of the capsaicin-sensitive vagal, but not the spinal, sensory innervation to the upper gastrointestinal tract significantly attenuated by 57% lipid-induced inhibition of gastric emptying. In intact rats, administration of a specific cholecystokinin (CCK)-A receptor antagonist, devazepide, significantly attenuated by 66% the response to lipid. Administration of devazepide in perivagal capsaicin-treated rats did not further reduce the response to lipid. These results suggest that lipid in the duodenum inhibits gastric emptying via a mechanism involving an action of CCK at type A receptors and capsaicin-sensitive vagal afferents.
authors.affiliation: Veterans Affairs/University of California Los Angeles Gastroenteric Biology Center.
authors.forename: H H
authors.initials: HH
authors.lastname: Hölzer
authors.forename: C M
authors.initials: CM
authors.lastname: Turkelson
authors.forename: T E
authors.initials: TE
authors.lastname: Solomon
authors.forename: H E
authors.initials: HE
authors.lastname: Raybould
mesh_terms: D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology*; D002766:Cholecystokinin / Q000097:blood / Q000502:physiology*; D020109:Devazepide; D004386:Duodenum / Q000378:metabolism; D005746:Gastric Emptying / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008055:Lipids / Q000502:physiology*; D008297:Male; D009475:Neurons, Afferent / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology*
chemical_list: D001570:Benzodiazepinones; D008055:Lipids; D011949:Receptors, Cholecystokinin; D002766:Cholecystokinin; D020109:Devazepide; D002211:Capsaicin