Superior late patency of small-diameter Dacron grafts seeded with omental microvascular cells: an experimental study.
The purpose of the study was to investigate the effect of omental microvascular cell seeding on the patency of small-diameter Dacron prostheses usable for coronary artery bypass grafting. In a canine carotid artery model, each dog (n = 64) received one seeded and one similar nonseeded Dacron prosthesis (internal diameter = 4 or 6 mm). Enzymatically harvested omental microvascular cells (omentum = 27.6 +/- 5.9 g [+/- the standard deviation]; range, 17 to 50 g) were seeded prior to implantation. The seeding density was 1.91 +/- 0.26 [+/- the standard error] x 10(6) cells/cm2 of graft surface. Dipyridamole (75 mg/d) and acetylsalicylic acid (325 mg/d) were administered orally for 4 weeks postoperatively. The prostheses were explanted between 2 and 52 weeks after placement. The results were assessed by angiography; light, scanning electron, and transmission electron microscopy; and morphometry. The seeded grafts developed a uniform luminal monolayer of endothelial cells with minimal platelet or cellular deposition. These grafts also had a significantly higher overall patency rate and significantly larger thrombus-free surface areas than the nonseeded grafts. The overall actuarial patency rates at 1 week, 5, 12, 26, and 52 weeks were 100%, 98%, 93%, 93%, and 93%, respectively, for seeded Dacron grafts and 100%, 91%, 61%, 54%, and 18%, respectively, for nonseeded grafts. The patency rates of Dacron grafts usable for coronary artery bypass grafting are significantly improved by seeding with omental microvascular cells in a canine model.
Pasic
M
M
Department of Surgery, University Hospital Zurich, Switzerland.
Müller-Glauser
W
W
von Segesser
L K
LK
Lachat
M
M
Mihaljevic
T
T
Turina
M I
MI
D000206:Actuarial Analysis; D000818:Animals; D001807:Blood Vessel Prosthesis / Q000295:instrumentation* / Q000379:methods; D002339:Carotid Arteries / Q000601:surgery*; D002455:Cell Division; D017690:Cell Transplantation; D001026:Coronary Artery Bypass / Q000295:instrumentation* / Q000379:methods; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D006085:Graft Survival; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D009852:Omentum; D011093:Polyethylene Terephthalates; D019736:Prostheses and Implants; D011474:Prosthesis Design; D013499:Surface Properties; D013997:Time Factors; D014654:Vascular Patency
D011093:Polyethylene Terephthalates