title: In vitro repair of oxidative and ultraviolet-induced DNA damage in supercoiled nucleoid DNA by human cell extract.
abstract: A human cell extract is capable of carrying out damage-specific incision on supercoiled nucleoid DNA. The DNA breaks are detected by single cell gel electrophoresis. Incubation of the incised DNA with extract and DNA precursors results in resealing of the breaks, with retention of the supercoiled state of the DNA.
authors.affiliation: Boyd Orr Research Centre, Rowett Research Institute, Bucksburn, Aberdeen, UK.
authors.forename: A R
authors.initials: AR
authors.lastname: Collins
authors.forename: I M
authors.initials: IM
authors.lastname: Fleming
authors.forename: C M
authors.initials: CM
authors.lastname: Gedik
mesh_terms: D002474:Cell-Free System; D004249:DNA Damage; D004260:DNA Repair; D004278:DNA, Superhelical / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D010084:Oxidation-Reduction; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D004278:DNA, Superhelical; D006861:Hydrogen Peroxide