[Experimental study on cytoplasmic free calcium and antigen presentation function of peritoneal macrophage following severe scald].
A dynamic observation of cytoplasmic free calcium concentration (PM phi [Ca2+]i) and antigen presentation (AP) function was made in severely scalded mice. The results showed that [Ca2+]i of unstimulated PM phi in vitro increased significantly at 12, 24 and 72h postburn (PB), and the [Ca2+]i of PM phi stimulated by A23187 in vitro was also increased but not statistically significant at the same time points. These results suggested that PM phi was activated in vivo, even in a highly or over activated state. Severe scald caused the obvious depression of AP function of PM phi. This phenomenon, not only appearing very early but also persisting for a long time, might become the key factor of suppression of specific immune function PB. The changes in M phi activity in vivo might be the basic event of the M phi dysfunction after severe burns.
Peng
D
D
Burn Research Institute, Southwest Hospital, Third Military Medical College, Chongqing.
Huang
W
W
Li
A
A
D000118:Acetylglucosaminidase / Q000097:blood; D000818:Animals; D017951:Antigen Presentation; D002056:Burns / Q000276:immunology*; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D051379:Mice
D015220:Calcium Channels; D004247:DNA; D000118:Acetylglucosaminidase; D002118:Calcium