Detection of stress proteins in Porphyromonas gingivalis and other oral bacteria by western immunoblotting analysis.
Detection of stress proteins in Porphyromonas gingivalis was investigated by SDS-PAGE and Western immunoblotting procedure using a polyclonal antibody (anti hsp60) and a monoclonal antibody (anti-Dnak). Results indicate that P. gingivalis can elicit a hsp60-like stress protein when submitted to different environmental stresses such as a heat shift from 35 degrees C to 43 degrees C, a pH drop from 7.2 to 6.0 or an increase in oxygen concentration. Virulent and non-virulent strains of P. gingivalis responded the same way. The other bacterial species tested also showed an increased synthesis of a GroEL-like protein after heat shock, as detected by the anti hsp60 antibody. However, the monoclonal anti-Dnak recognized an hsp70-like protein only in two of the tested species.
Vayssier
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Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Québec, Canada.
Mayrand
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Grenier
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D
D000328:Adult; D015153:Blotting, Western; D018834:Chaperonin 60 / Q000302:isolation & purification; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D006358:Hot Temperature; D006801:Humans; D008970:Molecular Weight; D009055:Mouth / Q000382:microbiology; D010518:Periodontitis / Q000209:etiology; D016966:Porphyromonas gingivalis / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
D018834:Chaperonin 60; D006360:Heat-Shock Proteins