A method for the detection of protein-bound mutagens in food.
To investigate the possible presence of protein-bound mutagens in food an analytical procedure has been devised in which the sample is enzymically hydrolysed, fractionated by HPLC and examined by a modified liquid incubation Ames assay. To validate the method MeIQx was added, as a model compound, to beefburger and a recovery of 82% obtained. The limit of detection for protein-bound mutagens was 1 microgram/kg, expressed as equivalents of MeIQx. No detectable mutagenicity was observed when the procedure was applied to samples of well cooked beefburger, irradiated chicken or mycoprotein.
Ibe
F I
FI
BIBRA Toxicology International, Carshalton, Surrey, UK.
Blowers
S D
SD
Anderson
D
D
Massey
R
R
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004798:Enzymes; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D006868:Hydrolysis; D008460:Meat; D009152:Mutagenicity Tests; D009153:Mutagens / Q000302:isolation & purification*; D011810:Quinoxalines / Q000302:isolation & purification
D004798:Enzymes; D009153:Mutagens; D011810:Quinoxalines