Involvement of S100-related calcium-binding protein pEL98 (or mts1) in cell motility and tumor cell invasion.
We examined the relationship between cell motility and the expressions of pEL98 (mts1) mRNA and protein in various murine normal and transformed cells. The expression of pEL98 (mts1) in v-Ha-ras-transformed NIH3T3 cells and in normal rat kidney cells transformed by either v-Ha-ras or v-src was increased over that in the corresponding parental cells at both mRNA and protein levels. The expression in normal rat fibroblasts (3Y1) transformed by v-Ha-ras was also increased compared with that in 3Y1 cells. However, the expression of pEL98 (mts1) in 3Y1 cells transformed by v-src was increased in one clone (src 3Y1-K), but decreased in another clone (src 3Y1-H). The expression level of pEL98 (mts1) correlated well with cell motility, which was examined by measuring cell tracks by phagokinesis. In order to test direct involvement of the pEL98 (mts1) protein in cell motility, src 3Y1-H cells that showed low cell motility were transfected with pEL98 cDNA. The transfectants expressing large amounts of the pEL98 protein showed significantly higher cell motility than src 3Y1-H cells. The expression of pEL98 (mts1) was also found to be correlated with motile and invasive abilities in various clones derived from Lewis lung carcinoma. These results suggest that the pEL98 (mts1) protein plays a role in regulating cell motility and tumor cell invasiveness.
Takenaga
K
K
Division of Chemotherapy, Chiba Cancer Center Research Institute.
Nakamura
Y
Y
Endo
H
H
Sakiyama
S
S
D016475:3T3 Cells; D000818:Animals; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D018827:Carcinoma, Lewis Lung / Q000378:metabolism; D002461:Cell Line, Transformed; D002465:Cell Movement; D002471:Cell Transformation, Neoplastic; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D011905:Genes, ras; D016391:Genes, src; D007668:Kidney; D051379:Mice; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins
D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C470944:S100a4 protein, mouse; C499412:S100a4 protein, rat