title: Prolonged neonatal myocardial preservation with a highly buffered low-calcium solution.
abstract: Optimal methods of myocardial preservation remain controversial in the neonate. This study compared prolonged hypothermic storage of neonatal hearts with modified University of Wisconsin solution (group I) with a solution formulated to promote anaerobic glycolysis by providing proton buffering with histidine (100 mmol/L) and exogenous glucose and insulin (group II). Hearts from piglets aged 3 to 5 days were given a single dose of either solution (n = 6 each), subjected to 20 hours of global ischemia at 4 degrees C, and reperfused with an erythrocyte-enhanced perfusate in an isovolumic Langendorff preparation. After 1 hour of reperfusion, in comparison with hearts preserved with University of Wisconsin solution, those in group II demonstrated (mean +/- standard error of the mean) greater left ventricular developed pressure (101 +/- 7 versus 62 +/- 9 mm Hg, p < 0.01) and the first derivative of left ventricular pressure (816 +/- 23 versus 614 +/- 69 mm Hg.sec-1, p < 0.05). Diastolic indices were reduced to a similar degree in the two groups. Myocardial oxygen consumption was significantly greater (38.8 +/- 2.4 versus 11.8 +/- 2.4 microliters oxygen.min.g-1, p < 0.01) in group II hearts. Group I hearts vasoconstricted (6% increase in coronary vascular resistance) in response to an intracoronary infusion of acetylcholine (20 nmol.min-1); in contrast, acetylcholine produced coronary dilation in group II hearts (5% decrease in coronary resistance, p < 0.02) that was similar to that observed in nonischemic control hearts. These results demonstrate enhanced preservation of myocardial contractility, oxidative metabolism, and vascular function in neonatal hearts provided by a solution designed to buffer protons and promote anaerobic glycolysis during ischemia.
authors.affiliation: Department of Anesthesiology/CCM, University of Pittsburgh School of Medicine, Pa.
authors.forename: F X
authors.initials: FX
authors.lastname: McGowan
authors.forename: H
authors.initials: H
authors.lastname: Cao-Danh
authors.forename: K
authors.initials: K
authors.lastname: Takeuchi
authors.forename: P J
authors.initials: PJ
authors.lastname: Davis
authors.forename: P J
authors.initials: PJ
authors.lastname: del Nido
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002314:Cardioplegic Solutions; D003331:Coronary Vessels / Q000502:physiology; D006019:Glycolysis; D006321:Heart; D009924:Organ Culture Techniques; D009926:Organ Preservation / Q000379:methods*; D013552:Swine; D014655:Vascular Resistance
chemical_list: D002314:Cardioplegic Solutions