A comparison of ELISA and RPLA for detection of Bacillus cereus diarrhoeal enterotoxin.
Fourteen strains of Bacillus cereus isolated from different sources were examined for their ability to produce diarrhoeal enterotoxin by two commercial immunoassay kits (Oxoid BCET-RPLA and Tecra ELISA) and the microslide immunodiffusion assay. One strain that was positive in monkey feedings, as well as a number of other strains isolated from diarrhoeal outbreaks, gave positive results in the ELISA and negative results in the RPLA test systems. When tested in the microslide assay, these strains produced only one antigen which formed a line of identity with the reference toxin. The results of the control toxins provided with the kits substantiated that the two commercial assays did not detect the same antigen. Cultures positive with both assay kits were shown to produce diarrhoeal enterotoxin (by a line of identity) and other antigens in the microslide immunodiffusion assay.
Day
T L
TL
Department of Food Science and Nutrition, University of Minnesota, St Paul 55108.
Tatani
S R
SR
Notermans
S
S
Bennett
R W
RW
D000942:Antigens, Bacterial / Q000276:immunology; D001409:Bacillus cereus / Q000737:chemistry* / Q000276:immunology; D003967:Diarrhea / Q000382:microbiology; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D005779:Immunodiffusion; D011933:Reagent Kits, Diagnostic
D000942:Antigens, Bacterial; D004768:Enterotoxins; D011933:Reagent Kits, Diagnostic; C040792:enterotoxin, Bacillus cereus