Assignment of the true and processed genes for human glycine decarboxylase to 9p23-24 and 4q12.
Southern analysis using a human glycine decarboxylase cDNA probe and genomic DNA preparations from Chinese hamster-human hybridoma cell lines demonstrated that on the segregated human chromosomes, 4 and 9, there are glycine decarboxylase cDNA-related sequences. The finding is confirmatory of the fact that fragments of both the true and processed genes for this protein have been cloned. Since one of four HindIII fragments revealed in the present Southern analysis matched requirements for the property expected from the restriction map reported for the processed gene, this was located on chromosome 4. The three remaining signals were consequently ascribed to fragments from the true gene on chromosome 9. Fluorescence in situ hybridization using the genomic clones assigned the true and processed genes to 9p23-24 and 4q12, respectively.
Isobe
M
M
Department of Patho-Biochemistry, Research Institute for Oriental Medicine, Toyama, Japan.
Koyata
H
H
Sakakibara
T
T
Momoi-Isobe
K
K
Hiraga
K
K
D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D002899:Chromosomes, Human, Pair 9; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000302:isolation & purification; D015342:DNA Probes; D015247:Deoxyribonuclease HindIII; D005260:Female; D050841:Glycine Dehydrogenase (Decarboxylating); D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D005810:Multigene Family; D010920:Placenta / Q000201:enzymology; D015183:Restriction Mapping
D015342:DNA Probes; D004247:DNA; D000594:Amino Acid Oxidoreductases; D050841:Glycine Dehydrogenase (Decarboxylating); D015247:Deoxyribonuclease HindIII