title: First and second derivative spectrophotometric determination of cefoperazone and sulbactam in injections.
abstract: A simple, spectrophotometric assay to measure the concentrations of cefoperazone and sulbactam in injectable formulations is described. Since zero-order spectra are subject to interference, derivative spectrophotometry was used to enhance the spectral details. A linear relationship between derivative amplitudes and the concentrations of the compounds was found. Beer's law is obeyed up to 75 and 80 micrograms ml-1 of cefoperazone in the first and second derivative modes, respectively, and up to 75 micrograms ml-1 of sulbactam in the second derivative mode. Detection limits were 0.64 and 0.88 microgram ml-1, respectively for cefoperazone in the first and second derivative modes and 0.30 micrograms ml-1 for sulbactam in the second derivative mode. The method is rapid, simple, does not require a separation step and has successfully been applied to the assay of commercial injections containing cefoperazone and sulbactam.
authors.affiliation: Servicio de Farmacia, Hospital Virgen del Castillo, Murcia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Parra
authors.forename: J
authors.initials: J
authors.lastname: Garcia-Villanova
authors.forename: V
authors.initials: V
authors.lastname: Rodenas
authors.forename: M D
authors.initials: MD
authors.lastname: Gomez
mesh_terms: D000892:Anti-Infective Agents, Urinary / Q000737:chemistry* / Q000378:metabolism; D002438:Cefoperazone / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D004304:Dosage Forms; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet; D013407:Sulbactam / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000892:Anti-Infective Agents, Urinary; D004304:Dosage Forms; D004338:Drug Combinations; D002438:Cefoperazone; C057923:sulperazone; D013407:Sulbactam