title: High-performance liquid chromatographic determination of dietary fibre in raw and processed carrots.
abstract: An HPLC method was developed to determine dietary fibre in carrots (Daucus carota L.). Primary hydrolysis of dietary fibre residue was performed with 12 M H2SO4 for 2 h at 40 degrees C and secondary hydrolysis with 0.414 M H2SO4 for 3 h at 100 degrees C. For the neutralization step prior to injection, AG4-X4 resin (Bio-Rad) was used. Neutral monosaccharides were separated using an HPX-87P column (Bio-Rad). This method was applied for evaluation of the quantitative variation of dietary fibre content in carrots during autoclaving.
authors.affiliation: Departamento de Nutrición y Bromatología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Redondo
authors.forename: M J
authors.initials: MJ
authors.lastname: Villanueva
authors.forename: M D
authors.initials: MD
authors.lastname: Rodríguez
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018552:Daucus carota / Q000737:chemistry*; D004043:Dietary Fiber / Q000032:analysis*; D006868:Hydrolysis; D011134:Polysaccharides / Q000032:analysis; D013464:Sulfuric Acids
chemical_list: D002241:Carbohydrates; D004043:Dietary Fiber; D011134:Polysaccharides; D013464:Sulfuric Acids; C033158:sulfuric acid