title: The toxic effect of Alzheimer amyloid protein precursor overexpressed in the neuroblastoma cell line NB-1 on neurite outgrowth.
abstract: The neuroblastoma cell line NB-1 is induced to start neurite outgrowth by dibutyryl cyclic AMP (Bt2cAMP). To study the function of Alzheimer amyloid protein precursor (APP), a stable cell line overexpressing APP was established by cDNA transfection. The APP cDNA was driven by the cytomegalovirus early gene promoter. The transformant underwent degeneration as well as neurite outgrowth in the presence of Bt2cAMP, which also increased the amount of APP mRNA and protein. The expressed APP was mainly processed in the secretory pathway and few amyloidogenic fragments were observed. Hence, not only the beta/A4 protein but also the overexpressed APP itself might be neurotoxic.
authors.affiliation: Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Sciences, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: Y
authors.initials: Y
authors.lastname: Kumagae
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: W
authors.initials: W
authors.lastname: Yamao-Harigaya
authors.forename: M
authors.initials: M
authors.lastname: Usami
authors.forename: S
authors.initials: S
authors.lastname: Ishiura
authors.forename: S
authors.initials: S
authors.lastname: Kawashima
authors.forename: K
authors.initials: K
authors.lastname: Obata
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000502:physiology*; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D006801:Humans; D016501:Neurites / Q000502:physiology*; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger; D003994:Bucladesine