title: pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos.
abstract: We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1 alpha-encoding gene (EF-1 alpha). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.
authors.affiliation: Department of Zoology, University of Texas at Austin 78712.
authors.forename: A D
authors.initials: AD
authors.lastname: Johnson
authors.forename: P A
authors.initials: PA
authors.lastname: Krieg
mesh_terms: D000818:Animals; D001755:Blastocyst; D003001:Cloning, Molecular; D046508:Culture Techniques; D004625:Embryo, Nonmammalian; D005822:Genetic Vectors; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic; D014982:Xenopus laevis
chemical_list: D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012333:RNA, Messenger