A novel cationic amphiphile for transfection of mammalian cells.
We describe here a new cationic amphiphile, N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine (diC14-amidine), which interacts with plasmid DNA and generates hydrophobic stable complexes resistant against DNase I. In partition experiments between two non-miscible phases, DNA was transferred into an organic phase upon complex formation with diC14-amidine-containing vesicles. Finally, vesicles made of a diC14-amidine and phosphatidylethanolamine (PE) (1:1, mol:mol) mixture or pure diC14-amidine were efficient in mediating transfection of adherent (CHO) and suspension (K562) cell lines, using the chloramphenicol acetyltransferase (CAT) gene as reporter.
Ruysschaert
J M
JM
Laboratoire de Chimie Physique des Macromolécules aux Interfaces CP 206/2, Université Libre de Bruxelles, Belgium.
el Ouahabi
A
A
Willeaume
V
V
Huez
G
G
Fuks
R
R
Vandenbranden
M
M
Di Stefano
P
P
D000578:Amidines; D000818:Animals; D016466:CHO Cells; D002412:Cations; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000096:biosynthesis; D006224:Cricetinae; D004247:DNA / Q000008:administration & dosage* / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D008081:Liposomes; D008322:Mammals; D010957:Plasmids; D013997:Time Factors; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
D000578:Amidines; D002412:Cations; D007202:Indicators and Reagents; D008081:Liposomes; C089665:N-t-butyl-n'-tetradecyl-3-tetradecylaminopropionamidine; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase