Characterization of the mouse Rep-3 gene: sequence similarities to bacterial and yeast mismatch-repair proteins.
The mouse Rep-3 gene is transcribed divergently from the same promoter region as the dihydrofolate reductase-encoding gene and has a deduced amino-acid sequence that shares identity with the bacterial protein, MutS, which is involved in DNA mismatch repair. We have cloned Rep-3, mapped it and sequenced all of the known exons and their intron junction sequences. We find that the open reading frame is considerably larger than initially reported and that the most abundant form of Rep-3 mRNA encodes a protein of 123 kDa. The gene spans at least 134 kb and consists of 26 exons, including several alternatively spliced exons. All of the exon/intron junctions match the expected consensus sequences with the exception of the splice junctions for intron 6, which has AT and AC dinucleotides instead of the usual GT and AG bordering the exon sequences. The junction sequences for this intron share consensus sequences with three intron sequences from other genes, thereby helping to establish an alternative consensus sequence.
Liu
K
K
Department of Biology, Emory University, Atlanta, GA 30322.
Niu
L
L
Linton
J P
JP
Crouse
G F
GF
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004247:DNA; D004260:DNA Repair; D005091:Exons; D005656:Fungal Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D000074081:MutS Homolog 3 Protein; D009692:Nucleic Acid Heteroduplexes; D011506:Proteins / Q000235:genetics*; D012326:RNA Splicing
D001426:Bacterial Proteins; D005656:Fungal Proteins; C089762:Msh3 protein, mouse; D000074081:MutS Homolog 3 Protein; D009692:Nucleic Acid Heteroduplexes; D011506:Proteins; D004247:DNA