title: In vitro antibiotic susceptibilities of Borrelia isolates from erythema migrans lesion of Lyme disease patients in Japan.
abstract: Antibiotic susceptibilities of twelve borrelial isolates from skin of patients with erythema migrans (EM) and ticks (Ixodes persulcatus and I. ovatus) in Japan were examined by in vitro microdilution MIC method and macrodilution MBC method. Nine EM isolates and 3 tick isolates were susceptible to amoxicillin, erythromycin, and minocycline. MICs for Japanese isolates were 0.038-0.30 microgram/ml, < 0.012 microgram/ml, and < 0.012-0.05 microgram/ml, respectively. MBCs were as follows: 0.038-0.88 microgram/ml, < 0.012-0.10 microgram/ml, and < 0.025-0.78 microgram/ml, respectively. These antibiotics could be recommended for treatment of patients in early stage of Lyme disease in Japan.
authors.affiliation: Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Masuzawa
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Kawabata
authors.forename: Y
authors.initials: Y
authors.lastname: Yanagihara
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology*; D000818:Animals; D001898:Borrelia / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D015787:Erythema Chronicum Migrans / Q000188:drug therapy / Q000382:microbiology*; D004917:Erythromycin / Q000494:pharmacology*; D005858:Germany; D006801:Humans; D007564:Japan; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000494:pharmacology*; D012867:Skin / Q000382:microbiology; D013987:Ticks / Q000382:microbiology; D014481:United States
chemical_list: D004917:Erythromycin; D000658:Amoxicillin; D008911:Minocycline