Modulation of LDL catabolism by sodium nitroprusside and PGE2 in human peripheral blood lymphocytes.
The effect of nitric oxide donor--sodium nitroprusside (NaNP) and PGE2 on the LDL-receptor activity and LDL cellular accumulation by isolated human blood lymphocytes was investigated. Preincubation of lymphocytes with lipoprotein deficient medium (LPDS) resulted in the increase of the LDL-receptor activity and the LDL-cellular accumulation. NaNP (30-300 microM) dose-dependently prevented the increase of the LDL-receptor activity as well as the accumulation of LDL by lymphocytes. However, in "starwing" cells (cells with high LDL-receptor activity) the effect of NaNP on the receptor activity was biphasic. At concentration up to 100 microM NaNP inhibited, while at a concentration of 300 microM, it activated the LDL-receptor activity. PGE2 (3-30 microM) inhibited LDL catabolism, however, this effect was hardly concentration-dependent.
Miszczuk-Jamska
B
B
Department of Clinical Biochemistry Collegium Medicum Jagiellonian University, Cracow, Poland.
Wybrańska
I
I
Hartwich
J
J
Dembińska-Kieć
A
A
Gryglewski
R J
RJ
D015232:Dinoprostone / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology*; D014461:Ultracentrifugation; D015854:Up-Regulation / Q000187:drug effects
D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL; D009599:Nitroprusside; D015232:Dinoprostone