[The functional activity of the regulator region of virus SV40 integrated into recombinant plasmids].
We propose the system for transfection of eucaryotic SV40-based vectors. The method comprises simultaneous transfection of permissive monkey cells with plasmid DNA and the virus SV40. It is likely that the induction of replication of recombinant plasmids by the SV40 T-antigen is an indicator of functional activity of the SV40 regulatory region integrated into recombinant plasmids.
Landau
S M
SM
Kolomiets
L I
LI
Tikhonov
A V
AV
D000818:Animals; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D000882:Haplorhini; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection / Q000235:genetics
D004279:DNA, Viral
Izuchenie funktsional'noĭ aktivnosti reguliatornogo raĭona virusa SV40 v sostave rekombinantnykh plazmid.