title: Genetic exchange in Trypanosoma brucei: evidence for meiosis from analysis of a cross between drug-resistant transformants.
abstract: Genetic exchange in Trypanosoma brucei spp. can occur when two strains are cotransmitted through the tsetse fly vector, but it is non-obligatory and a comparatively rare event. To increase recovery of hybrids, we crossed drug resistant parental strains and selected hybrids by double drug resistance [15]. Analysis of 29 hybrid clones from five separate genetic exchange events shows independent segregation of marker genes and a high frequency of triploidy, both of which phenomena have been observed previously for other trypanosome crosses. However, in addition we provide evidence of genetic recombination involving the tubulin locus. These three observations strongly support the hypothesis that genetic exchange starts with a meiotic division in T. brucei.
authors.affiliation: Department of Pathology and Microbiology, University of Bristol Veterinary School, Langford, UK.
authors.forename: W
authors.initials: W
authors.lastname: Gibson
authors.forename: M
authors.initials: M
authors.lastname: Bailey
mesh_terms: D000483:Alleles; D000818:Animals; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D018105:DNA, Kinetoplast / Q000235:genetics; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D017125:Genes, Protozoan; D005819:Genetic Markers; D007621:Karyotyping; D008297:Male; D008540:Meiosis / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011123:Polyploidy; D014170:Transformation, Genetic; D014346:Trypanosoma brucei brucei / Q000187:drug effects / Q000235:genetics*
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D005819:Genetic Markers