Molecular cloning and chromosome localization of a putative basolateral Na(+)-K(+)-2Cl- cotransporter from mouse inner medullary collecting duct (mIMCD-3) cells.
Electroneutral Na(+)-K(+)-2Cl- cotransporters represent one of the major routes for Cl- movement in epithelia. A secretory form of the cotransporter has been described in the basolateral membrane of a variety of epithelia from fish to mammals. We isolated a putative bumetanide-sensitive Na(+)-K(+)-2Cl- cotransporter cDNA, BSC2, from mIMCD-3 cells. Northern analysis indicates that in contrast to BSC1, the recently cloned renal-specific apical isoform of the cotransporter, BSC2 is expressed in secretory epithelia and thus appears to represent the basolateral isoform. Furthermore, BSC2 is also expressed in non-polarized cells, such as red cells and myocytes. Sequence comparison and chromosome localization demonstrate that BSC2 and BSC1 are different genes that diverged before the evolution of vertebrates.
Delpire
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Harvard Center for Study of Kidney Disease, Renal Division, Harvard Medical School, Boston, Massachusetts.
Rauchman
M I
MI
Beier
D R
DR
Hebert
S C
SC
Gullans
S R
SR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D007679:Kidney Medulla / Q000166:cytology; D007685:Kidney Tubules, Collecting / Q000166:cytology; D051379:Mice; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011188:Potassium / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D012964:Sodium / Q000378:metabolism*; D028021:Sodium-Potassium-Chloride Symporters
D002352:Carrier Proteins; D002712:Chlorides; D012333:RNA, Messenger; D028021:Sodium-Potassium-Chloride Symporters; D012964:Sodium; D011188:Potassium