A novel fucosylated glycosphingolipid from the millipede, Parafontaria laminata armigera.
A novel fucosylated glycosphingolipid (GL-3a) was isolated and purified from whole tissues of the millipede, Parafontaria laminata armigera. Its chemical structure was characterized as Man beta 1-4(Fuc alpha 1-3)Glc beta 1-ceramide (I3 alpha Fuc,MlOse2Cer) by gas-liquid chromatography, permethylation study, partial acid hydrolysis, exoglycosidase degradation, TLC/enzyme-immunostaining, negative fast atom bombardment-mass spectrometry and proton nuclear magnetic resonance spectroscopy. This compound was unique in containing a fucose branch linked to the glucose residue of the disaccharide, mannosylglucose. The ceramide moiety was mainly composed of d17:1 (64.3%) and d18:1 (20.0%) sphingoids, and 22:0 (41.8%), 23:0 (16.4%) and 24:0 (15.8%) fatty acids.
Sugita
M
M
Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University, Japan.
Hayata
C
C
Yoshida
T
T
Suzuki
M
M
Suzuki
A
A
Takeda
T
T
Hori
T
T
Nakatani
F
F
D000818:Animals; D001181:Arthropods / Q000737:chemistry*; D002240:Carbohydrate Sequence; D005643:Fucose / Q000737:chemistry*; D006028:Glycosphingolipids / Q000737:chemistry / Q000302:isolation & purification*; D008745:Methylation; D008969:Molecular Sequence Data; D014281:Trihexosylceramides / Q000737:chemistry
D006028:Glycosphingolipids; D014281:Trihexosylceramides; C090437:mannosyl-1-4-(fucosyl-1-3)glucosyl-1-ceramide; D005643:Fucose