Inhibition of feline immunodeficiency virus gene expression and replication by alphaherpesvirus ICP4 homologues.
We investigated the effects of pseudorabies virus (PRV), herpes simplex virus type 1 (HSV-1), and equine herpesvirus type 1 (EHV-1) ICP4 homologues on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression. This was done by using the transient expression chloramphenicol acetyltransferase (CAT) assay in Crandell feline kidney (CRFK) and Felis catus whole fetus 4 cells transfected with a chimeric FIV LTR-CAT reporter construct in combination with effector plasmids expressing the PRV, HSV-1 or EHV-1 ICP4 homologue. The experiments demonstrated that the ICP4 homologues could significantly inhibit FIV LTR-directed gene expression. Moreover, the ICP4 homologues also exhibited a marked inhibitory effect on FIV replication in CRFK cells cotransfected with an infectious molecular clone of FIV.
Kawaguchi
Y
Y
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Maeda
K
K
Miyazawa
T
T
Ono
M
M
Tsubota
K
K
Tomonaga
K
K
Mikami
T
T
D000818:Animals; D001483:Base Sequence; D002415:Cats; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000096:biosynthesis; D005333:Fetus; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism; D006736:Horses; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000378:metabolism*; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000502:physiology*; D007668:Kidney; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D018139:Simplexvirus / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection; D014779:Virus Replication
D017874:Immediate-Early Proteins; D014157:Transcription Factors; C048925:herpes simplex virus, type 1 protein ICP4; D015500:Chloramphenicol O-Acetyltransferase