Sequence, mapping and disruption of CCC1, a gene that cross-complements the Ca(2+)-sensitive phenotype of csg1 mutants.
We have isolated, sequenced, mapped and disrupted a novel gene, CCC1, from Saccharomyces cerevisiae. This gene displays non-allelic complementation of the Ca(2+)-sensitive phenotype conferred by the csg1 mutation. The ability of this gene, in two copies per cell, to reverse the csg1 defect suggests it may have a role in regulating Ca2+ homeostasis. The sequence of CCC1 indicates that it encodes a 322 amino acid, membrane-associated protein. The CCC1 gene is located on the right arm of chromosome XII.
Fu
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Department of Biochemistry, Uniformed Services University of the Health Science, Bethesda, Maryland 20814.
Beeler
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Dunn
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology*; D027682:Cation Transport Proteins; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010641:Phenotype; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
C089360:CCC1 protein, S cerevisiae; D027682:Cation Transport Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D002118:Calcium