Heat shock protein produced by Helicobacter pylori.
The cells of Helicobacter pylori were suspended in the medium containing 35S-methionine. After a heat shock of the cells at 42 C for 5, 10, and 30 min, the production of proteins was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Out of many proteins produced by the cells, only 66 kDa protein production was dramatically increased by heat treatment. The N-terminal amino acid sequence of 66 kDa protein was quite similar to that of 62 kDa and 54 kDa proteins previously suggested as heat shock protein (HSP) of H. pylori based on the reaction with polyclonal and monoclonal antibodies against HSP 60 family proteins produced by other bacteria. Therefore, it was concluded that H. pylori produces the 66 kDa protein as its major heat shock protein which belongs to HSP 60 family.
Yokota
K
K
Department of Bacteriology, Okayama University Medical School, Japan.
Hirai
Y
Y
Haque
M
M
Hayashi
S
S
Isogai
H
H
Sugiyama
T
T
Nagamachi
E
E
Tsukada
Y
Y
Fujii
N
N
Oguma
K
K
D000595:Amino Acid Sequence; D001345:Autoradiography; D018834:Chaperonin 60 / Q000096:biosynthesis*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D016480:Helicobacter pylori / Q000378:metabolism*; D006358:Hot Temperature; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D013462:Sulfur Radioisotopes
D018834:Chaperonin 60; D003470:Culture Media; D013462:Sulfur Radioisotopes; D008715:Methionine