title: Glycosphingolipids in cultured lens epithelial cells from dog and rhesus monkey.
abstract: Vertebrate lens tissues contain several species of acidic and neutral glycosphingolipids in relatively high amounts. However, the epithelia with capsule from dog and rhesus monkey lenses had a simpler composition and lower content of glycosphingolipids than whole lenses. Gangliosides and neutral glycosphingolipids in monolayer cultures of lens epithelial cells were also different from those in whole lenses. Although alpha-galactosyl (Gal alpha 1-3Gal-R) or Lewis(x) (Gal beta 1-4[Fuc alpha 1-3]GlcNAc-R) epitopes were found in glycosphingolipids from whole lenses, they were not detected in those from monolayer cultures of dog and rhesus monkey lens cells. In addition, significant changes in ganglio-series gangliosides were induced in monolayer cultures of both cells, where GM3 and GD3 were predominant. Immunofluorescence study revealed a characteristic distribution of cell surface gangliosides in confluent monolayers. These findings suggest that glycosphingolipid synthesis in lens epithelia is intrinsically different from that in cortical and nuclear fibres, and that the expression of Lewis(x) and alpha-galactosyl epitopes in glycosphingolipids appears to be associated with the differentiation of epithelial cells to fibres.
authors.affiliation: PRESTO, Research Development Corporation of Japan (JRDC), Tokyo.
authors.forename: M
authors.initials: M
authors.lastname: Ogiso
authors.forename: M
authors.initials: M
authors.lastname: Ohta
authors.forename: T
authors.initials: T
authors.lastname: Okinaga
authors.forename: M
authors.initials: M
authors.lastname: Hoshi
authors.forename: M
authors.initials: M
authors.lastname: Komoto
authors.forename: K
authors.initials: K
authors.lastname: Asano
authors.forename: M
authors.initials: M
authors.lastname: Takehana
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D003459:Crystallins / Q000032:analysis; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000737:chemistry; D005677:G(M1) Ganglioside / Q000032:analysis; D005679:G(M3) Ganglioside / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005732:Gangliosides / Q000032:analysis; D006028:Glycosphingolipids / Q000032:analysis*; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000737:chemistry*; D016256:Lewis X Antigen / Q000032:analysis; D008253:Macaca mulatta; D008969:Molecular Sequence Data
chemical_list: D003459:Crystallins; D005679:G(M3) Ganglioside; D005732:Gangliosides; D006028:Glycosphingolipids; D016256:Lewis X Antigen; D005677:G(M1) Ganglioside; C026226:ganglioside, GD3; D005690:Galactose