Influence of Pall Pl-50 platelet filters on lactate dehydrogenase, platelet factor 4, and complement factor 3 in stored platelet concentrates.
BACKGROUND
The study examines Pall Pl-50 platelet filters with respect to their influence on some biochemical properties of platelet concentrates.


METHODS
Ten pairs of pooled platelet preparations, each consisting of 3 platelet concentrates, were studied. On day 1, one of the preparations was filtered and the other served as an identical non-filtered control. Immediately after filtration platelet counts, the levels of desarginated activated complement factor 3 (C3a), extracellular levels of lactate dehydrogenase (LDH) and extracellular and total concentrations of platelet factor 4 (PF4) were determined in both concentrates. After 5 days sterility testing and determination of extracellular levels of PF4 and LDH were performed.


RESULTS
Extracellular concentrations of PF4 and C3a decreased immediately after filtration (p < 0.01). Furthermore, Pall Pl-50 filters caused cellular instability reflected by an increased release of PF4 (p < 0.01) during subsequent storage.


CONCLUSIONS
Platelet concentrates filtered with Pall Pl-50 would not appear suitable for storage.
Järemo
P
P
Department of Transfusion Medicine, Orebro Medical Center Hospital, Sweden.
D001793:Blood Preservation; D002469:Cell Separation / Q000295:instrumentation; D015169:Colony Count, Microbial; D015926:Complement C3a / Q000378:metabolism*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000378:metabolism*; D017713:Platelet Transfusion / Q000295:instrumentation*; D010983:Plateletpheresis / Q000295:instrumentation*
D010978:Platelet Factor 4; D015926:Complement C3a; D007770:L-Lactate Dehydrogenase