Comparative 3H-CCK-8S binding studies on CCKB-type receptors in guinea-pig cortex and continuous cell lines.
The present study was undertaken to compare binding characteristics of CCKB-type receptors in guinea-pig cortex, Jurkat T-cells, GH3 cells and C6 cells. The rank order of potency of a variety of CCK agonists and antagonists in inhibiting specific [3H]CCK-8S binding was highly correlated for the 4 CCKB receptor models as demonstrated by a computer-assisted statistical analysis. Taking the ligand binding profiles as the criterion it is concluded that CCKB receptors in guinea-pig cortex, Jurkat T-cells, pituitary GH3 cells and rat glioma C6 cells share identical pharmacological properties.
Kaufmann
R
R
Institute of Pharmacology and Toxicology, Medical Faculty (Charité), Humboldt University of Berlin, Germany.
Schöneberg
T
T
Henklein
P
P
Boomgaarden
M
M
Sohr
D
D
Ott
T
T
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D002540:Cerebral Cortex / Q000378:metabolism*; D002766:Cholecystokinin / Q000819:agonists*; D005910:Glioma; D006168:Guinea Pigs; D007700:Kinetics; D010903:Pituitary Gland, Anterior; D051381:Rats; D011949:Receptors, Cholecystokinin / Q000378:metabolism*; D012844:Sincalide / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014316:Tritium; D014407:Tumor Cells, Cultured
D011949:Receptors, Cholecystokinin; D014316:Tritium; D002766:Cholecystokinin; D012844:Sincalide