The LSP1 gene is expressed in cultured normal and transformed mouse macrophages.
The mouse LSP1 protein is an F-actin binding protein initially isolated as a cDNA from the BALB/c pre-B cell line 220.2. Its expression pattern is highly restricted. Only lymphocytes and lymphoma cell lines were reported to express LSP1. Non-lymphoid cell lines or normal mouse tissues such as brain, lung, liver, skeletal muscle, kidney or testis do not express LSP1. Here we report that mouse macrophage cell lines also express LSP1 mRNA and protein. DNA sequence analysis shows that the coding sequence of LSP1 RNA expressed in the macrophage cell line P388D1 is identical to the sequence of LSP1 RNA from the pre-B cell line 220.2. To determine the expression of LSP1 RNA in normal macrophages derived from fetal liver and adult bone marrow and in other hematopoietic cells we used a recently described technique to make representative amplified polyA cDNA samples from small numbers of cells or isolated hematopoietic colonies. Analysis of these cDNA samples with an LSP1 cDNA probe showed that eight of nine macrophage samples expressed LSP1 RNA. One of two neutrophil samples but none of eight other non-lymphoid colonies was positive for LSP1 RNA. From these results it appears that the expression of LSP1 RNA in the hematopoietic system is restricted to the lymphocyte, macrophage and neutrophil lineages.
Jongstra
J
J
Arthritis Centre-Research Unit, Toronto Hospital Research Institute, Ontario, Canada.
Ittel
M E
ME
Iscove
N N
NN
Brady
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D001854:Bone Marrow Cells; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D002460:Cell Line; D018076:DNA, Complementary / Q000302:isolation & purification; D015870:Gene Expression; D008099:Liver / Q000166:cytology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; C057701:Lsp1 protein, mouse; D008840:Microfilament Proteins; D012333:RNA, Messenger