Genomic fingerprinting of Yersinia enterocolitica species by degenerate oligonucleotide-primed polymerase chain reaction.
A novel version of the polymerase chain reaction (PCR) termed degenerate oligonucleotide-primed PCR (DOP-PCR) was used to fingerprint the bacterial genome of Yersinia enterocolitica species. Eight well-characterized reference strains of Y. enterocolitica (ribotype, serotype, biotype and zymotype) were examined. Optimal experimental conditions for reproducibility, sensitivity and specificity were obtained using a single DOP-primer. All the strains gave distinct DOP-PCR profiles composed of 12 to 19 fragments with sizes from 200 to 1.500 bp. Thus, the DOP-PCR, which has so far been used to fingerprint human, mouse, fruitfly and plant DNA, is also well-suited to bacterial DNA.
Sayada
C
C
Laboratoire de Biochimie Génétique, Hôpital Robert Debré, Paris, France.
Picard
B
B
Elion
J
J
Krishnamoorthy
R
R
D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015008:Yersinia enterocolitica / Q000235:genetics*
D017931:DNA Primers