The effect of electromagnetic field stimulation on corticosteroids-inhibited intestinal wound healing.
Electromagnetic field (EMF) stimulation has been used successfully in the clinical setting to promote healing of ununited fractures. In a few studies, EMF stimulation enhanced soft tissue healing. To investigate the effect of EMF stimulation on intestinal wound healing in normal rats and in those treated with corticosteroids, 80 Wistar rats received twice-daily injections of either saline dexamethasone (0.1 mg/kg/day for 2 weeks. Animals then underwent creation of single-layer, inverting small intestine anastomoses. All injections were continued postoperatively. Animals were grouped as intestinal anastomoses; intestinal anastomoses plus EMF stimulation; intestinal anastomoses plus dexamethasone; and intestinal anastomoses plus dexamethasone plus EMF. On postoperative days 7 and 14, the anastomosed intestines were removed and the tensile strength (TS) and hydroxyproline (OH-P) contents measured. EMF stimulation significantly increased intestinal wound healing in normal animals by the 7th and 14th day. Corticosteroids significantly impaired the healing of the small intestine anastomoses, with decreased TS and OH-P contents after the first and second weeks. However, EMF stimulation significantly reversed this inhibitory effect.
Dindar
H
H
Department of Pediatric Surgery, School of Medicine, Ankara University, Türkiye.
Renda
N
N
Barlas
M
M
Akinay
A
A
Yazgan
E
E
Tinçer
T
T
Cakmak
M
M
Konkan
R
R
Gökçora
I H
IH
Yücesan
S
S
D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology; D004599:Electric Stimulation Therapy; D006909:Hydroxyproline / Q000378:metabolism; D007082:Ileum / Q000293:injuries* / Q000502:physiology / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
D003907:Dexamethasone; D006909:Hydroxyproline