The increase in gene expression induced by introduction of rare codons into the C terminus of the template.
Short oligodeoxynucleotides (oligos) possessing two tandem Arg codons followed by TGA stop codon were inserted near the 3' end of a modified cat gene. It was found that while being decoded in vivo, the AGGAGGTGA oligo increased the yield of gene product and, in addition, caused -1 frameshifting. The 3-10-fold increase of the yield of the polypeptide was accompanied by increased accumulation of corresponding mRNA, indicating a protection from messenger decay. Transformation of the cells by a plasmid overproducing tRNA(4Arg) gene compensates for all the anomalies.
Gursky
Y G
YG
Institute of Experimental Cardiology, Cardiology Research Center, Moscow, Russia.
Beabealashvilli
R Sh
RSh
D000595:Amino Acid Sequence; D001120:Arginine / Q000235:genetics*; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003062:Codon / Q000235:genetics*; D018388:Codon, Terminator / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D016368:Frameshift Mutation / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012347:RNA, Transfer, Arg / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013698:Templates, Genetic; D014158:Transcription, Genetic
D003062:Codon; D018388:Codon, Terminator; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D012347:RNA, Transfer, Arg; D011993:Recombinant Fusion Proteins; D001120:Arginine; D015500:Chloramphenicol O-Acetyltransferase