Use of Yarrowia lipolytica hexokinase for the quantitative determination of trehalose 6-phosphate.
This paper describes a procedure for the quantitative determination of trehalose 6-phosphate (T6P) based on its ability to inhibit hexokinase from Yarrowia lipolytica. The assay is linear between 1 nmol and at least 8 nmol. The concentration of T6P in wild-type Saccharomyces cerevisiae (0.15 mM) and in ras2 mutants (0.25 mM) remained unchanged in the exponential or stationary phase of growth or after heat shock. A tps1 mutant affected in T6P synthase did not show detectable T6P. Heat shock increased the concentration of T6P in Schizosaccharomyces pombe from 0.43 to 0.75 mM.
Blázquez
M A
MA
Instituto de Investigaciones Biomédicas C.S.I.C., Madrid, Spain.
Gancedo
J M
JM
Gancedo
C
C
D006593:Hexokinase / Q000032:analysis*; D006358:Hot Temperature; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D004718:Saccharomycetales / Q000201:enzymology*; D013403:Sugar Phosphates / Q000032:analysis*; D014199:Trehalose / Q000031:analogs & derivatives* / Q000032:analysis; D015003:Yeasts / Q000201:enzymology*
D013403:Sugar Phosphates; C082722:trehalose-6-phosphate; D014199:Trehalose; D006593:Hexokinase