An upper limit for the effect of low frequency magnetic fields on ATP-sensitive potassium channels.
Currents have been recorded for ATP-sensitive potassium channels in excised patches of membranes from an insulin secreting cell line, CRI-G1. The multi-channel records have been analyzed to reveal the single-channel conductance, the frequency and duration of bursts and the frequency of flickers (with periods between 0.5 and 5 ms). Control records in the absence of applied magnetic fields are similar to those reported by others. Patches have been exposed to parallel static and low frequency magnetic fields including a combination satisfying the 'cyclotron resonance' condition. The fields were applied for 30 s periods interleaved with 30 s controls. No significant differences in channel properties were observed between the control and field exposed periods. The largest change in position of the peak of the distribution of opening and closing transitions was 3%.
Wang
K W
KW
Department of Pharmacology, University of Cambridge, UK.
Hladky
S B
SB
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002460:Cell Line; D008280:Magnetics; D015221:Potassium Channels / Q000502:physiology*
D015221:Potassium Channels; D000255:Adenosine Triphosphate