Electrophoretic separation of developmental and adult rabbit skeletal muscle myosin heavy chain isoforms: example of application to muscle denervation study.
We present the separation by SDS gel-electrophoresis of the six main myosin heavy chains (MHC) present in rabbit skeletal muscle. The separation of the four adult MHC (1, 2A, 2X/2D, 2B) was compared to that of the corresponding rat MHC as described by Talmadge and Roy [J. Appl. Physiol. 99 (1993) 2337-2340]. We found that many rabbit muscles contained mainly one of the four MHC, in some cases the 2B MHC. In addition, we resolved the embryonic E and perinatal P developmental MHC, which should facilitate muscle differentiation and regeneration studies in the rabbit. An example of application to the study of muscle denervation is given.
Janmot
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C
Laboratoire de Biologie Physicochimique, Université Paris-Sud, Orsay, France.
d' Albis
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A
D000818:Animals; D003714:Denervation; D004591:Electrophoresis, Polyacrylamide Gel; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000254:growth & development / Q000294:innervation; D009218:Myosins / Q000302:isolation & purification*; D011817:Rabbits; D051381:Rats
D009218:Myosins