title: Peroxisomal beta-oxidation. Purification of four novel 3-hydroxyacyl-CoA dehydrogenases from rat liver peroxisomes.
abstract: Peroxisomes are capable of beta-oxidizing a variety of substrates including the CoA esters of straight chain fatty acids, 2-methyl-branched fatty acids and the bile acid intermediates di- and trihydroxycoprostanic acids. The first reaction of peroxisomal beta-oxidation is catalyzed by an acyl-CoA oxidase. Rat liver peroxisomes contain three acyl-CoA oxidases: 1) palmitoyl-CoA oxidase, oxidizing straight chain acyl-CoAs; 2) pristanoyl-CoA oxidase, oxidizing 2-methyl-branched acyl-CoAs; and 3) trihydroxycoprostanoyl-CoA oxidase, oxidizing the CoA esters of the bile acid intermediates (Van Veldhoven, P.P., Vanhove, G., Asselberghs, S., Eyssen, H. J., and Mannaerts, G. P. (1992) J. Biol. Chem. 267, 20065-20074). We have now investigated whether the third step of peroxisomal beta-oxidation, catalyzed by a 3-hydroxyacyl-CoA dehydrogenase, is also catalyzed by multiple enzymes, using the 3-hydroxyacyl-CoA derivatives of palmitic acid, 2-methylpalmitic acid, and trihydroxycoprostanic acid as the substrates to monitor the dehydrogenase activities. In order to avoid contamination with mitochondrial 3-hydroxyacyl-CoA dehydrogenases, highly purified peroxisomes from untreated rats were employed as the enzyme source. Subfractionation of the peroxisomes revealed that the major portion of the dehydrogenase activities with all three substrates was present in the peripheral membrane protein fraction. Separation of this fraction on various chromatographic columns resulted in the purification of the well known multifunctional protein, a 78-kDa monomeric protein that displays 3-hydroxyacyl-CoA dehydrogenase plus hydratase activity, as well as of four additional novel dehydrogenases with different substrate specificities. Three of the enzymes are monomeric proteins of 35 kDa, 56 kDa, and 79 kDa, respectively. The latter enzyme also displays hydratase activity. The fourth enzyme is a dimer of 89 kDa, the subunits of which form a doublet at 40 kDa. The exact physiological role of each of the 3-hydroxyacyl-CoA dehydrogenases requires further investigation.
authors.affiliation: Department of Pharmacology, Katholieke Universiteit Leuven, Belgium.
authors.forename: D K
authors.initials: DK
authors.lastname: Novikov
authors.forename: G F
authors.initials: GF
authors.lastname: Vanhove
authors.forename: H
authors.initials: H
authors.lastname: Carchon
authors.forename: S
authors.initials: S
authors.lastname: Asselberghs
authors.forename: H J
authors.initials: HJ
authors.lastname: Eyssen
authors.forename: P P
authors.initials: PP
authors.lastname: Van Veldhoven
authors.forename: G P
authors.initials: GP
authors.lastname: Mannaerts
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000302:isolation & purification*; D000818:Animals; D002451:Cell Compartmentation; D002793:Cholic Acids / Q000378:metabolism; D003065:Coenzyme A / Q000737:chemistry; D008099:Liver / Q000201:enzymology*; D008297:Male; D008830:Microbodies / Q000201:enzymology*; D010084:Oxidation-Reduction; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D002793:Cholic Acids; D010169:Palmitic Acids; C034839:2-methylhexadecanoic acid; D019308:Palmitic Acid; D015094:3-Hydroxyacyl CoA Dehydrogenases; D003065:Coenzyme A