title: Acute action of choriogonadotropin on Leydig tumor cells: induction of a higher affinity benzodiazepine-binding site related to steroid biosynthesis.
abstract: We previously demonstrated that the mitochondrial peripheral-type benzodiazepine receptor (PBR) is coupled to hormone-activated steroidogenesis by regulating the intramitochondrial cholesterol transport, the rate-determining step of steroid biosynthesis. In the present study we examined whether PBR is the site of hormone action using the hCG-responsive MA-10 mouse Leydig tumor cell line as a model system. Within 15 sec of the addition of hCG to Leydig cells a 3-fold cAMP-dependent increase in PBR binding was observed. This rapid increase returned to basal levels within 60 sec. No effect was observed after 1 min in the continued presence of hCG. Scatchard analysis revealed that in addition to the known high affinity (5.0 nM) benzodiazepine-binding site, a second, hormone-induced, higher affinity (0.2 nM) benzodiazepine-binding site appeared. We then examined whether in such a short time frame steroid synthesis occurs. Fifteen-second incubation of MA-10 cells with the inhibitor of cholesterol metabolism aminoglutethimide together with hCG also resulted in an increased rate of pregnenolone formation by their isolated mitochondria that were washed and incubated in aminoglutethimide-free buffer. The dose response of benzodiazepine binding to hCG closely parallels the increase in steroid formation by the mitochondria of stimulated cells. Addition of the selective inhibitor of cAMP-dependent protein kinase, H-89, completely blocked hormone-induced PBR binding and steroid formation, whereas addition of the inactive analog H-85 was without any effect. The addition of flunitrazepam, a benzodiazepine previously shown to inhibit the trophic hormone action on steroidogenesis, completely abolished the hCG-induced rapid stimulation of steroid synthesis. These results demonstrate that in MA-10 cells, the most rapid effect described thus far of hCG and cAMP, is the transient induction of a higher affinity benzodiazepine-binding site, which occurs concomitantly with an increase in the rate of steroid formation. This, in turn, suggests that these hormones alter PBR to activate cholesterol delivery to the inner mitochondrial membrane and subsequent steroid formation.
authors.affiliation: Department of Cell Biology, Georgetown University Medical Center, Washington, D.C. 20007.
authors.forename: N
authors.initials: N
authors.lastname: Boujrad
authors.forename: J L
authors.initials: JL
authors.lastname: Gaillard
authors.forename: M
authors.initials: M
authors.lastname: Garnier
authors.forename: V
authors.initials: V
authors.lastname: Papadopoulos
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002118:Calcium / Q000032:analysis / Q000502:physiology; D002784:Cholesterol / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D007984:Leydig Cell Tumor / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D011963:Receptors, GABA-A / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D013256:Steroids / Q000378:metabolism*; D013736:Testicular Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D006063:Chorionic Gonadotropin; D011963:Receptors, GABA-A; D013256:Steroids; D002784:Cholesterol; D000242:Cyclic AMP; D002118:Calcium