title: Biosynthesis of erythromycin: origin of the methyl protons.
abstract: 13C-Nuclear magnetic resonance (13C-NMR) spectroscopy has been used to locate deuterium atoms incorporated biosynthetically into erythromycin from [3-13CD3]- and [3-13C]sodium propionate and L-[13CD3]- and L-[13C]methionine in Saccharopolyspora erythraea JCM 4748. The alpha-shifts of deuterated methyl groups were clearly observed in broad-band deuterium and proton-decoupled 13C-NMR spectra. The seven propionate-derived methyl groups and the four methionine-derived methyl groups were shown to undergo no exchange of methyl protons during the biosynthesis of erythromycin.
authors.affiliation: Department of Medicinal Chemistry, Meiji College of Pharmacy, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kajiwara
authors.forename: R
authors.initials: R
authors.lastname: Uegaki
authors.forename: K
authors.initials: K
authors.lastname: Iida
authors.forename: Y
authors.initials: Y
authors.lastname: Wada
mesh_terms: D004917:Erythromycin / Q000096:biosynthesis*; D009682:Magnetic Resonance Spectroscopy; D011522:Protons; D016930:Saccharopolyspora / Q000378:metabolism*
chemical_list: D011522:Protons; D004917:Erythromycin