Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis).
Fertilization of eggs from cynomolgus monkeys (Macaca fascicularis) was achieved in vitro using frozen-thawed spermatozoa. The best sperm survival rate was obtained after freezing spermatozoa in a semen-diluting medium (TTE: Tes, Tris, egg yolk base) containing 5% glycerol with 30 min equilibrium time. Although frozen-thawed spermatozoa could fertilize eggs in vitro and some of the fertilized eggs developed into blastocysts or hatched blastocysts, a sperm fertility assay using salt-stored eggs indicated that frozen-thawed spermatozoa had a shorter lifespan in capacitation medium than did fresh, unfrozen spermatozoa. This may be attributable to damage to their plasma membranes.
Sankai
T
T
Tsukuba Primate Center for Medical Science, National Institute of Health, Ibaraki, Japan.
Terao
K
K
Yanagimachi
R
R
Cho
F
F
Yoshikawa
Y
Y
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro; D008252:Macaca fascicularis; D008297:Male; D009865:Oocytes; D013048:Specimen Handling; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa
D003451:Cryoprotective Agents