Integrating the porcine physical and linkage map using cosmid-derived markers.
An essential part in the development of informative linkage maps is to include genetic markers that have been anchored by physical mapping. Here a set of 18 porcine cosmid-derived genetic markers are reported that have been mapped by linkage analysis, and that also have been physically localized by fluorescence in situ hybridization (FISH). Three different strategies were used to establish polymorphic markers from the cosmid clones. Firstly, dinucleotide microsatellite loci were derived by sequencing cosmid subclones containing (CA)n repeats. Secondly, variable SINE 3' poly(A) tracts (SINEVA) were identified by direct SINE-PCR amplification of cosmid clones. Thirdly, the cosmids were used in Southern blot hybridization to detect restriction fragment length polymorphisms (RFLPs). Compared with the most recent consensus compilation of the porcine gene map, the present assignment of markers to chromosomes 2p, 3, 4, 10, 12q, and 16 represents the first loci mapped to these chromosomes, for which linkage as well as in situ data are now available.
Ellegren
H
H
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala.
Chowdhary
B
B
Johansson
M
M
Andersson
L
L
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003360:Cosmids; D017931:DNA Primers / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D013552:Swine / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides