Solution conformation of enopeptin A, a depsipeptide antibiotic, using 2D NMR and restrained molecular dynamics studies.
Studies on the solution conformation of the cyclic depsipeptide antibiotic enopeptin A have been carried out using 2D NMR and molecular modelling techniques. The proton resonances of the antibiotic in DMSO-d6 have been assigned by the use of TOCSY and ROESY experiments. The interproton distance information obtained from the ROESY experiments have been used as the basis for elucidating the probable structures in solution. The restrained molecular dynamics technique was applied to calculate the structures in solution, and six resultant structures with fewer distance constraint violations were obtained that satisfy the experimental restraints very well. The conformation of the cyclic moiety of the molecules is well defined whereas the aliphatic chain segment is disordered.
Young
J J
JJ
Institute of Preventive Medicine, National Defence Medical Center, Taipei, Taiwan, R.O.C.
Jung
L J
LJ
Liu
W T
WT
Ho
S N
SN
Chang
L R
LR
Tsai
Y C
YC
Bhaskaran
R
R
Yu
C
C
D000595:Amino Acid Sequence; D003903:Deuterium; D004121:Dimethyl Sulfoxide; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008956:Models, Chemical; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry*; D011487:Protein Conformation; D012996:Solutions; D013057:Spectrum Analysis / Q000379:methods; D013816:Thermodynamics; D047630:Depsipeptides
D003903:Deuterium; D004121:Dimethyl Sulfoxide; D010456:Peptides, Cyclic; D012996:Solutions; C585143:acyldepsipeptides; D047630:Depsipeptides