title: Evaluation of the QBC method to detect malaria infections in field surveys.
abstract: The conventional peripheral blood film method used to diagnose malaria is characterized by low sensitivity in scanty parasitaemia and can be time consuming when required to rule out infection. The Quantitative Buffy Coat (QBC) method has been proposed to be quicker and more sensitive. We conducted a malaria survey in April 1992 among school-children in Kisumu (holoendemic) and Webuye (hypoendemic) areas of Western Kenya. Peripheral blood samples were examined by thick blood smear (TBS) stained with Giemsa solution, and by the QBC method. A total of 360 paired samples were analyzed. There were 175 (49%) positive TBS and 201 (56%) positive QBC. Of the 185 TBS classified as negative, 30 (16%) were positive by QBC. When parasite density by TBS was > or = 100/300 WBCs, the sensitivity of QBC was 100%. Overall sensitivity for QBC was 98%, with a specificity of 84%. Negative predictive value for the QBC was 98%, and had a calculated accuracy of 92%. It took an average of 44 minutes to process a TBS and a further average of 2.6 minutes to examine a negative TBS. For the QBC the mean time to process and to examine was 7.09 and 1.04 minutes respectively. We conclude that the QBC is quicker, with high sensitivity, and will prove useful in clinical and epidemiological screening, especially when parasitaemia is low.
authors.affiliation: Vector Biology and Control Research Centre, Kenya Medical Research Institute (KEMRI), Kisumu, Kenya.
authors.forename: A J
authors.initials: AJ
authors.lastname: Oloo
authors.forename: S O
authors.initials: SO
authors.lastname: Ondijo
authors.forename: I O
authors.initials: IO
authors.lastname: Genga
authors.forename: D A
authors.initials: DA
authors.lastname: Boriga
authors.forename: M L
authors.initials: ML
authors.lastname: Owaga
authors.forename: D
authors.initials: D
authors.lastname: Ngare
authors.forename: E
authors.initials: E
authors.lastname: Gathecha
mesh_terms: D001399:Azure Stains; D002648:Child; D002675:Child, Preschool; D005188:False Negative Reactions; D005189:False Positive Reactions; D006306:Health Surveys; D006801:Humans; D007630:Kenya / Q000453:epidemiology; D008288:Malaria / Q000097:blood* / Q000453:epidemiology / Q000469:parasitology / Q000517:prevention & control; D008403:Mass Screening / Q000379:methods*; D010274:Parasitology / Q000379:methods; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D013997:Time Factors
chemical_list: D001399:Azure Stains