Effects of dietary protein and uninephrectomy on renal angiotensin converting enzyme activity in the rat.
This study examines the effects of dietary protein and of uninephrectomy on angiotensin converting enzyme (ACE) in the normotensive rat, with particular regard to the kidney. Male Wistar Kyoto rats were fed isocaloric diets containing 5, 16 or 50% protein for three weeks. Other groups of rats were subjected to either left unilateral nephrectomy or sham operations, and the rats were killed eight days after surgery. ACE activity was measured in the kidney medulla, cortex, proximal tubule brush border membrane and in the plasma, heart and lung. Renal cortex and brush border ACE activity increased in parallel with protein intake, whereas plasma and lung ACE activity decreased; heart and kidney medulla ACE activity did not vary significantly. Uninephrectomy also led to a high increase in brush border ACE activity in the contralateral kidney, with no effect in the renal medulla or in the other tissues. The increase in ACE activity in the brush border membrane corresponded to a similar increase in the maximum number of binding sites of 3H-ramiprilat. This suggested that the increase in ACE activity corresponded to an increase in ACE concentration. The increase in renal tubular ACE activity could result in higher angiotensin II levels, and could consequently play a role in the modification of sodium reabsorption and cellular growth which occurs in the proximal tubule in these experimental models.
Michel
B
B
Institut de Pharmacologie, URA DO589 CNRS, Strasbourg, France.
Grima
M
M
Coquard
C
C
Welsch
C
C
Barthelmebs
M
M
Imbs
J L
JL
D000469:Alkaline Phosphatase / Q000378:metabolism; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000378:metabolism; D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology*; D008168:Lung / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D009392:Nephrectomy; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D017257:Ramipril / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D004044:Dietary Proteins; C052549:ramiprilat; D000469:Alkaline Phosphatase; D007703:Peptidyl-Dipeptidase A; D000254:Sodium-Potassium-Exchanging ATPase; D017257:Ramipril