Detection of the 10-kD antigen structural gene in mycobacteria by using the polymerase chain reaction.
The structural gene encoding the 10-kD antigen from Mycobacterium tuberculosis was amplified by the polymerase chain reaction. The 297-base-pair (bp) product was detected among 45 strains representing 14 mycobacterial species, but was absent from 11 species related to the mycobacteria. The gene was localized to a approximately 2000-bp SstII restriction fragment of the organisms' chromosomes.
Cooksey
R C
RC
Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333.
Crawford
J T
JT
D000942:Antigens, Bacterial / Q000235:genetics*; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016133:Polymerase Chain Reaction
D000942:Antigens, Bacterial