Purification of recombinant Gq alpha, G11 alpha, and G16 alpha from Sf9 cells.
Hepler
J R
JR
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
Kozasa
T
T
Gilman
A G
AG
D000818:Animals; D016367:Baculoviridae; D001921:Brain / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D015151:Immunoblotting / Q000379:methods; D007202:Indicators and Reagents; D007700:Kinetics; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D018411:Spodoptera; D010738:Type C Phospholipases / Q000096:biosynthesis / Q000302:isolation & purification
D018076:DNA, Complementary; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D010738:Type C Phospholipases; D019204:GTP-Binding Proteins