Purification and some properties of alpha-L-arabinofuranosidase from Bacillus subtilis 3-6.
alpha-L-Arabinofuranosidase (EC 3.2.1.55) was purified from culture supernatant of Bacillus subtilis 3-6. The enzyme had a molecular weight of 61,000 and displayed maximum activity at pH 7.0 and 60 degrees C. It released arabinose from O-alpha-L-arabinofuranosyl-(1-->3)-O-beta-D-xylopyranosyl-(1-->4)-D-x ylopyranos e (A1X2), O-beta-D-xylopyranosyl-(1-->4)-[O-alpha-L-arabinofuranosyl-(1-->3)]- O-beta-D-xylopyranosyl-(1-->4)-D-xylopyranose (A1X3), and arabinan, but not from O-beta-D-xylopyranosyl-(1-->2)-O-alpha-L- arabinofuranosyl-(1-->3)-O-beta-D-xylopyranosyl-(1-->4)-O-beta-D-xylopyr anosyl- (1-->4)-D-xylopyranose (A1X4), arabinoxylan, gum arabic, or arabinogalactan.
Kaneko
S
S
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Sano
M
M
Kusakabe
I
I
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D002240:Carbohydrate Sequence; D006026:Glycoside Hydrolases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
D000596:Amino Acids; D009844:Oligosaccharides; D011134:Polysaccharides; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase