title: Prolyl hydroxylase inhibitor and lysyl hydroxylase inhibitor inhibit spreading of corneal epithelium.
abstract: BACKGROUND
  The spreading of epithelium is critical in the healing of corneal wounds. Such epithelial spreading requires the continuous production of protein and glycoprotein. To determine whether collagen production is required for the spreading of corneal epithelium, we studied the effects of inhibitors for collagen production on spreading of corneal epithelium in vitro.
  
  
  METHODS
  We examined the effect of two proline analogs, L-azetidine 2-carboxylic acid and cis-hydroxyproline, a prolyl hydroxylase inhibitor, ethyl-3,4-dihydroxybenzoate, and a lysyl hydroxylase inhibitor, minoxidil, on the spreading of epithelium of organ-cultured rabbit cornea.
  
  
  RESULTS
  Both analogs and inhibitors inhibited epithelial spreading in a dose-dependent manner.
  
  
  CONCLUSIONS
  These observations indicate that collagen production may be involved in the spreading of corneal epithelium.
authors.affiliation: Department of Ophthalmology, Wakayama Medical College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saika
authors.forename: N
authors.initials: N
authors.lastname: Hashizume
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: S
authors.initials: S
authors.lastname: Kobata
authors.forename: O
authors.initials: O
authors.lastname: Yamanaka
authors.forename: K
authors.initials: K
authors.lastname: Uenoyama
authors.forename: A
authors.initials: A
authors.lastname: Ooshima
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000502:physiology; D003315:Cornea / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000502:physiology; D062385:Hydroxybenzoates / Q000494:pharmacology; D008914:Minoxidil / Q000494:pharmacology; D009924:Organ Culture Techniques; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000037:antagonists & inhibitors*; D011393:Procollagen-Proline Dioxygenase / Q000037:antagonists & inhibitors*; D011392:Proline / Q000031:analogs & derivatives; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011817:Rabbits
chemical_list: D062385:Hydroxybenzoates; D011500:Protein Synthesis Inhibitors; C018063:ethyl protocatechuate; D008914:Minoxidil; D003094:Collagen; D011392:Proline; D011393:Procollagen-Proline Dioxygenase; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase