Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1.
The incorporation of histone H1 into chromatin during embryogenesis directs the specific repression of the Xenopus oocyte 5S rRNA genes. An increase in histone H1 content specifically restricts TFIIIA-activated transcription, and a decrease in histone H1 within chromatin facilitates the activation of the oocyte 5S rRNA genes by TFIIIA. Variation in the amount of histone H1 in chromatin does not significantly influence somatic 5S rRNA gene transcription. Thus, the regulated expression of histone H1 during Xenopus development has a specific and dominant role in mediating the differential expression of the oocyte and somatic 5S rRNA genes. This example demonstrates that histones can exert dominant repressive effects on the transcription of a gene in vivo in spite of an abundance of transcription factors for that gene.
Bouvet
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Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Dimitrov
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Wolffe
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AP
D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006657:Histones / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008845:Microinjections; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D016337:RNA, Catalytic; D012333:RNA, Messenger; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*; D014982:Xenopus laevis
D002843:Chromatin; D006657:Histones; D016337:RNA, Catalytic; D012333:RNA, Messenger; D012341:RNA, Ribosomal, 5S; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors