Lymph node cell proliferation assay in guinea pigs for the assessment of sensitizing potentials of chemical compounds.
The efficacy of a lymph node cell proliferation assay in the guinea pig as a first stage screening method of predicting sensitizing potentials of chemicals was studied by using several haptens. Animals were sensitized by a single 24-hr occlusive patch (24 cp), intradermal injection (id) and a combination of id and 24 cp, at a concentration used for guinea pig conventional contact hypersensitivity assay methods. Control animals were treated with vehicle(s) only. Suspensions of the lymph node cells (LNC) were individually prepared and cultured with [3H]methyl thymidine ([3H]TdR). [3H]TdR incorporation was measured and a stimulation index (SI) was calculated as a ratio of the mean [3H]TdR incorporation in sensitized animals and the mean [3H]TdR incorporation in control animals. LNC sensitized by 24 cp with 2,4-dinitrochlorobenzene proliferated maximally and significantly at day 5, whereas this occurred at day 7 after id sensitization. Significant LNC proliferation and high SI values were obtained successively by a combination of 24 cp and id. Moreover, strongly sensitizing chemicals increased significant LNC proliferation (SI > 2.0); weakly to moderately sensitizing chemicals also induced significant LNC proliferation (SI = 1.3-1.7). On the other hand, a primary irritant, sodium dodecyl sulfate, failed to encourage LNC proliferation (SI approximately 1.0).
Kashima
R
R
Biological Science Laboratories, Kao Corporation, Tochigi, Japan.
Okada
J
J
Ikeda
Y
Y
D000171:Acrolein / Q000031:analogs & derivatives / Q000276:immunology; D000818:Animals; D001566:Benzocaine / Q000276:immunology; D004137:Dinitrochlorobenzene / Q000276:immunology; D005260:Female; D005557:Formaldehyde / Q000276:immunology; D006168:Guinea Pigs; D007428:Intradermal Tests; D008198:Lymph Nodes / Q000166:cytology* / Q000187:drug effects*; D008213:Lymphocyte Activation / Q000187:drug effects*; D009355:Neomycin / Q000276:immunology; D009532:Nickel / Q000276:immunology; D010328:Patch Tests; D010476:Perfume / Q000494:pharmacology; D010655:Phenylenediamines / Q000276:immunology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D004137:Dinitrochlorobenzene; D010476:Perfume; D010655:Phenylenediamines; D005557:Formaldehyde; D012967:Sodium Dodecyl Sulfate; C029938:nickel sulfate; D000171:Acrolein; D009532:Nickel; D009355:Neomycin; C012843:cinnamaldehyde; D001566:Benzocaine; C029728:4-phenylenediamine