Use of acridinium-ester-labeled DNA probes for identification of mycobacteria in Bactec 13A blood cultures.
AccuProbe tests for mycobacteria (Gen-Probe) cannot be performed directly on Bactec 13A cultures because of interfering substances. This problem can be circumvented by subculturing to Bactec 12B media. Artifactual chemiluminescence greater than the positive cutoff was seen in 15 of 19 13A cultures containing Mycobacterium avium complex compared with 0 of 19 in 12B subcultures. Of the subcultures, 89% were tested after overnight incubation.
Ephraim
D A
DA
Department of Laboratories, University Hospital, Stony Brook, New York.
Spitzer
E D
ED
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis; D016470:Bacteremia / Q000175:diagnosis*; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D015342:DNA Probes; D006801:Humans; D008163:Luminescent Measurements; D015269:Mycobacterium avium Complex / Q000302:isolation & purification*; D015270:Mycobacterium avium-intracellulare Infection / Q000175:diagnosis; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D014376:Tuberculosis / Q000175:diagnosis
D003470:Culture Media; D015342:DNA Probes