Characterization of cord blood stem/progenitor cells (high proliferative potential colony-forming cells).
A population of very primitive cells has been purified from umbilical-cord blood. These primitive cells appear to be the equivalent of murine, high-proliferative potential, colony-forming cells (HPP-CFC). This analogy is based on the size of the colonies (1 to > 2.5 mm in diameter) and the replating potential of up to the fifth generation of individual colonies. At a single-cell level and in the presence of serum, increasing numbers of cytokines resulted in increasing numbers of HPP-CFC colonies. No HPP-CFC colonies were detected in the absence of serum, suggesting that additional cytokines are required for formation of these high-proliferative potential colonies.
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Walther Oncology Center, Indiana University, Indianapolis 46202.
D001790:Blood Physiological Phenomena; D002469:Cell Separation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn
D003470:Culture Media