title: Expression of epidermal growth factor receptor in the preimplantation uterus and blastocyst of the western spotted skunk.
abstract: The western spotted skunk is unique in that its blastocysts undergo a 180-220-day period of arrested development before implantation. We investigated the potential role of epidermal growth factor (EGF)-related growth factors in regulating uterine and embryonic development in this species by studying the status of EGF receptor (EGF-R) in these tissues during delayed implantation and resumption of embryonic development. The cell-specific distribution of EGF binding sites and the expression of EGF-R mRNA were assayed by autoradiography and Northern blot analysis, respectively. The size of EGF-R was determined by affinity cross-linking studies, and its bioactivity was examined by determining EGF-dependent subcellular protein tyrosine kinase (PTK) activity. EGF binding sites were localized in the uterine luminal and glandular epithelium, endometrial stroma, myometrium, and blood vessels during both stages of pregnancy. As examined by Northern blot hybridization, a cRNA probe specific to mouse EGF-R hybridized to poly(A)+ RNA of skunk uteri. Transcripts similar to those of mouse uterine EGF-R were identified. [125I]-EGF was cross-linked to a 170-kDa protein both in the uterus and in blastocysts collected during the delayed implantation and periimplantation periods. However, EGF-induced PTK activity was significantly elevated above background levels during the period of renewed embryonic development, but not during arrested embryonic development. The results suggest that EGF-related growth factors may play an important role in regulating embryonic development in this species and that a change in the number and/or functional status of the EGF-R may be a prerequisite for blastocyst activation and implantation in the spotted skunk.
authors.affiliation: Department of Obstetrics & Gynecology, University of Kansas Medical Center, Kansas City.
authors.forename: B C
authors.initials: BC
authors.lastname: Paria
authors.forename: S K
authors.initials: SK
authors.lastname: Das
authors.forename: R A
authors.initials: RA
authors.lastname: Mead
authors.forename: S K
authors.initials: SK
authors.lastname: Dey
mesh_terms: D000818:Animals; D001345:Autoradiography; D001755:Blastocyst / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000235:genetics / Q000502:physiology; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D012889:Mephitidae / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015347:RNA Probes; D014599:Uterus / Q000378:metabolism*
chemical_list: D015347:RNA Probes; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases