title: Molecular characterization of a cloned human oxytocin receptor.
abstract: We describe here the binding and functional properties of a cloned human oxytocin receptor (OTR). We established a transient OTR expression system on COS-1 cells, which do not express vasopressin receptors. With the transfected cells and [3H]oxytocin, the dissociation constant (Kd) of OTR to oxytocin was 6.0 +/- 1.1 nmol/l; the binding properties of several oxytocin-related peptides were also examined. The functional properties of OTR were determined by an electrophysiological method, using a Xenopus laevis oocyte injected with in vitro transcribed OTR mRNA. These two methods showed that [Phe2,Orn8]vasotocin, a vasopressin agonist, was an OTR antagonist. A combination of these methods using cloned OTR cDNA is a novel and effective method for the investigation of oxytocin-related ligands.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: Y
authors.initials: Y
authors.lastname: Makino
authors.forename: F
authors.initials: F
authors.lastname: Saji
authors.forename: M
authors.initials: M
authors.lastname: Takemura
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kubota
authors.forename: C
authors.initials: C
authors.lastname: Azuma
authors.forename: T
authors.initials: T
authors.lastname: Nobunaga
authors.forename: Y
authors.initials: Y
authors.lastname: Tokugawa
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D009865:Oocytes / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D018045:Receptors, Oxytocin / Q000032:analysis* / Q000235:genetics* / Q000502:physiology; D014316:Tritium; D014982:Xenopus laevis
chemical_list: D012333:RNA, Messenger; D018045:Receptors, Oxytocin; D014316:Tritium; D004247:DNA