Expression and purification of G-protein alpha subunits using baculovirus expression system.
Graber
S G
SG
Department of Pharmacology and Toxicology, West Virginia University, Morgantown 26505.
Figler
R A
RA
Garrison
J C
JC
D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D002460:Cell Line; D002845:Chromatography / Q000379:methods; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D046508:Culture Techniques / Q000379:methods; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D011865:Radioisotope Dilution Technique; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D018411:Spodoptera; D013462:Sulfur Radioisotopes; D014162:Transfection / Q000379:methods
D046911:Macromolecular Substances; D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D017886:Durapatite; D019204:GTP-Binding Proteins