title: Activation of Raf-1 by 14-3-3 proteins.
abstract: The protein Raf-1, a key mediator of mitogenesis and differentiation, associates with p21ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood. Using the yeast two-hybrid system, we identified two structurally related proteins that interact with the aminoterminal region of Raf-1. These proteins, 14-3-3 zeta (PLA2) and 14-3-3 beta (HS1), are members of the 14-3-3 family of proteins. Expression of 14-3-3 proteins in Xenopus oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.
authors.affiliation: Daiichi Research Center, Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco 94143.
authors.forename: W J
authors.initials: WJ
authors.lastname: Fantl
authors.forename: A J
authors.initials: AJ
authors.lastname: Muslin
authors.forename: A
authors.initials: A
authors.lastname: Kikuchi
authors.forename: J A
authors.initials: JA
authors.lastname: Martin
authors.forename: A M
authors.initials: AM
authors.lastname: MacNicol
authors.forename: R W
authors.initials: RW
authors.lastname: Gross
authors.forename: L T
authors.initials: LT
authors.lastname: Williams
mesh_terms: D048948:14-3-3 Proteins; D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002478:Cells, Cultured; D004789:Enzyme Activation; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009865:Oocytes; D009866:Oogenesis / Q000235:genetics / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014446:Tyrosine 3-Monooxygenase; D014981:Xenopus
chemical_list: D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D014446:Tyrosine 3-Monooxygenase; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf; D016203:CDC2 Protein Kinase