title: Characterization of Na(+)-dependent L-glutamate transport in canine erythrocytes.
abstract: Characteristics of the high-affinity Na(+)-dependent L-glutamate transport system in canine erythrocytes were studied by using intact cells and resealed ghosts. The L-glutamate transport showed a precise dependence on extracellular Na+ and intracellular K+. Kinetical analysis revealed that two Na+ ions and one K+ ion were involved in each L-glutamate transport cycle. The L-glutamate transport was inhibited most potently by threo-3-hydroxyaspartate and L-cysteinesulfinate (at 25 microM, 83% and 79% inhibition, respectively) and weakly by dihydrokainate and DL-alpha-aminoadipate (at 25 microM, 21% and 17% inhibition, respectively). From these stoichiometrical and pharmacological properties we concluded that the L-glutamate transport system in canine erythrocytes is a product of the L-glutamate transporter gene family and resembles a neuronal transporter rather than a glial one. L-Glutamate uptake was increased by internal, but not external, HCO3- when the internal and external anions of the erythrocytes were replaced by several other anions. Moreover, this enhancement was blocked by inhibition of carbonic anhydrase, which indicated that L-glutamate transport was at least partly dependent on HCO3- generated inside the cells. These observations indicate that anion countertransport is coupled to the high-affinity Na(+)- and K(+)-dependent L-glutamate transport in canine erythrocytes.
authors.affiliation: Department of Veterinary Internal Medicine, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Inaba
authors.forename: Y
authors.initials: Y
authors.lastname: Maede
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001692:Biological Transport; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*
chemical_list: D001639:Bicarbonates; D018698:Glutamic Acid; D012964:Sodium; D011188:Potassium