title: Evidence against involvement of APC mutation in papillary thyroid carcinoma.
abstract: Papillary thyroid carcinoma (PTC) is one of several tumours associated with familial adenomatous polyposis (FAP), an inherited tumour syndrome which appears to result from germ-line mutation of the APC tumour suppressor gene. Here we investigate the possibility that somatic mutation of APC might play a role in sporadic PTC. 16 cases of PTC together with matched normal tissue were examined by single-strand conformation polymorphism (SSCP) analysis, concentrating on the mutation cluster region (MCR) of the APC gene (codons 1286-1513). No evidence of mutation was observed in any sample. We conclude that APC mutation, at least in the MCR, is not a significant causal mechanism in sporadic PTC.
authors.affiliation: Department of Pathology, University of Edinburgh, U.K.
authors.forename: L
authors.initials: L
authors.lastname: Curtis
authors.forename: A H
authors.initials: AH
authors.lastname: Wyllie
authors.forename: J J
authors.initials: JJ
authors.lastname: Shaw
authors.forename: G T
authors.initials: GT
authors.lastname: Williams
authors.forename: A
authors.initials: A
authors.lastname: Radulescu
authors.forename: C
authors.initials: C
authors.lastname: DeMicco
authors.forename: D R
authors.initials: DR
authors.lastname: Haugen
authors.forename: J E
authors.initials: JE
authors.lastname: Varhaug
authors.forename: J R
authors.initials: JR
authors.lastname: Lillehaug
authors.forename: D
authors.initials: D
authors.lastname: Wynford-Thomas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D002291:Carcinoma, Papillary / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D005784:Gene Amplification; D017491:Genes, APC / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013964:Thyroid Neoplasms / Q000235:genetics*