Establishment of nickel-specific T cell lines from patients with allergic contact dermatitis: comparison of different protocols.
Allergic contact dermatitis is a delayed-type hypersensitivity reaction, secondary to hapten-specific T lymphocyte activation in sensitized individuals. The present study reports on the establishment of T cell lines from peripheral blood mononuclear cells (PBMC) of nickel-allergic patients, initially cultured with nickel, IL-2, or PHA and IL-2. It was possible to derive hapten-specific T cell lines from the three protocols, and the best proliferative responses to nickel were observed when PBMC were cultured in the presence of nickel in vitro. T cell lines initially cultured with IL-2 always gave better specific proliferative responses to nickel than those derived with PHA and IL-2. Phenotypical analysis of the nickel-specific T cell lines showed that they were mainly composed of activated CD8+ TcR alpha beta + T lymphocytes. These results emphasize the importance of initial culture conditions for the generation of hapten-specific T cell lines and suggest that CD8+ lymphocytes could play an important role in the pathogenesis of allergic contact dermatitis.
Bour
H
H
INSERM U346, Hôpital Edouard Herriot, Lyon, France.
Nicolas
J F
JF
Garrigue
J L
JL
Demidem
A
A
Schmitt
D
D
D016516:CD4-CD8 Ratio; D002460:Cell Line; D017449:Dermatitis, Allergic Contact / Q000097:blood / Q000209:etiology / Q000473:pathology*; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics; D009532:Nickel / Q000009:adverse effects*; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology
D009532:Nickel