Characterization of Staphylococcus intermedius from healthy dogs and cases of superficial pyoderma by DNA restriction endonuclease patterns.
In order to study the possible clonal relation of Staphyloccocus (S.) intermedius from canine superficial pyoderma and from healthy carriers, isolates from pustular swabs and from vaginal, nasal and normal skin sabs were typed using macrorestriction analysis with Sma I and pulsed-field gel electrophoresis. From the size of the resulting fragments the size of the chromosome of S. intermedius could be determined to be roughly 1500 +/- 200 kb on the average. The fingerprints were very heterogeneous though characteristically distinct from patterns of (human) S. aureus as published by others. Strains from superficial pyoderma were not found to be more similar to each other than strains from healthy carriers. Therefore it was concluded that strains from this type of skin infection probably did not belong to a certain subpopulation of S. intermedius, which might have indicated a higher virulence of these strains.
Department of Microbiology and Infectious Diseases, School of Veterinary Medicine, Hannover, Germany.
J
J
Hesselbarth
W
W
Witte
C
C
Cuny
R
R
Rohde
G
G
Amtsberg
D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D004262:DNA Restriction Enzymes / Q000032:analysis*; D004269:DNA, Bacterial; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D011711:Pyoderma / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes