Crystallization and preliminary X-ray analysis of a mutant duck delta II crystallin.
A duck delta II crystallin mutant, where histidine 178 has been replaced by an aspartic acid residue, has been purified from a bacterial expression system and subsequently crystallized. The crystals grow as flat plates, with unit cell dimensions a = 94.1 A, b = 99.9 A, c = 108.7 A and beta = 102 degrees. The crystals exhibit the symmetry of space group P2(1) and diffract to a minimum d-spacing of 2.8 A resolution. On the basis of density calculation four monomers (one tetramer) are estimated to be present in the asymmetric unit (Vm = 2.5 A3/Da). Self-rotation functions clearly show the presence of 222 non-crystallographic symmetry.
Division of Biochemistry Research, Hospital for Sick Children, Toronto, Ontario, Canada.
M
M
Abu-Abed
M A
MA
Turner
J
J
Atkinson
K
K
Dole
P L
PL
Howell
D000818:Animals; D003459:Crystallins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004372:Ducks; D009154:Mutation
D003459:Crystallins