Molecular cloning and characterization of the aklavinone 11-hydroxylase gene of Streptomyces peucetius subsp. caesius ATCC 27952.
The gene encoding aklavinone 11-hydroxylase of Streptomyces peucetius subsp. caesius ATCC 27952 was cloned and sequenced. The deduced amino acid sequence of the gene contains at least two common motifs of well-conserved amino acid sequences of several flavin-type bacterial hydroxylases. The hydroxylase gene is apparently transcribed from a single transcriptional start point. The phenotype of a dnrF mutant generated by gene disruption supports the idea that the dnrF gene encodes aklavinone 11-hydroxylase.
Hong
Y S
YS
Genetic Engineering Research Institute, Korea Institute of Science and Technology, Taejon.
Hwang
C K
CK
Hong
S K
SK
Kim
Y H
YH
Lee
J J
JJ
D000595:Amino Acid Sequence; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005182:Flavin-Adenine Dinucleotide; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D009243:NAD; D009279:Naphthacenes / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D009279:Naphthacenes; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; C027832:aklavinone; C090407:aklavinone 11-hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases