Production and characterization of a monoclonal antibody against bovine haptoglobin and its use in an ELISA.
Haptoglobin (Hp) is the major acute phase reactant found in cattle. As such, it is an excellent indicator of early disease processes and could be used as a marker for pre-clinical illness in cattle. The production of monoclonal antibodies (mAbs) directed against bovine Hp and bovine hemoglobin is described. The anti-haptoglobin mAbs (Hap1, Hap2, Hap3) and the anti-bovine hemoglobin (Hb) mAb (BoHem1) were characterized and tested for cross-reactivity by means of enzyme-linked immunosorbent assay (ELISA) and immunoblotting analyses. Additionally, the development of an ELISA based on an anti-haptoglobin mAb is discussed.
US Department of Agriculture, Agricultural Research Service, College Station, TX 77845.
C L
CL
Sheffield
C
C
Kamps-Holtzapple
J R
JR
DeLoach
L H
LH
Stanker
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D002417:Cattle; D004586:Electrophoresis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006242:Haptoglobins / Q000276:immunology*; D006454:Hemoglobins / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting; D007132:Immunoglobulin Isotypes / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D006242:Haptoglobins; D006454:Hemoglobins; D007132:Immunoglobulin Isotypes