Analysis of the developmental and transcriptional potentiation functions of 5'HS2 of the murine beta-globin locus control region in transgenic mice.
We analyze the role of 5'HS2 of the mouse beta-globin LCR in the transcriptional and developmental regulation of beta-globin gene expression. Previous studies have shown that the human beta-globin gene behaves as an adult gene in transgenic mice, being expressed in fetal liver and bone marrow-derived erythroblasts but not in yolk sac-derived embryonic erythroid cells. We show that linkage of mLCR5'HS2 to a human beta-globin gene alters this pattern of expression during ontogeny, resulting in expression of the linked beta-globin gene at all stages of murine erythroid development. Expression was independent of integration position and correlated with transgene copy number. Our results provide the first test of a phylogenetically homologous LCR in transgenic mice and demonstrate evolutionary conservation of both developmental and transcriptional potentiation functions between mammalian beta-globin LCRs.
Enver
T
T
Leukaemia Research Fund Centre at the Institute of Cancer Research, Chester Beatty Laboratories, London, United Kingdom.
Li
Q
Q
Gale
K B
KB
Hu
M
M
May
G E
GE
Karlinsey
J E
JE
Jimenez
G
G
Papayannopoulou
T
T
Costantini
F
F
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000502:physiology; D017931:DNA Primers / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental; D005914:Globins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D017931:DNA Primers; D005914:Globins