[Analysis of Ki-ras in pancreatic fluid aspirate. A new diagnostic possibility in investigation of pancreatic cancer].
The prognosis for pancreatic carcinoma is generally poor. The chance of survival could be improved if curative surgery were performed, but early diagnosis is then essential. Fine-needle aspiration cytology and endoscopic retrograde cholepancreaticography (ERCP) are widely used in order to obtain a precise diagnosis before laparotomy. In almost all patients with pancreatic adenocarcinomas, the oncogene Ki-ras is activated by point mutation. We describe a patient where conventional diagnostic procedures were inconclusive. However, DNA-analysis of aspirate from the pancreatic duct and from a pancreatic cyst showed activated Ki-ras oncogene. A malignant diagnosis was later confirmed. We propose that DNA-analyses of pancreatic fluid or tissues should be used whenever facing problems with an early and accurate diagnosis of pancreatic lesions.
Senter for klinisk molekylaermedisin, Haukeland Sykehus, Bergen.
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Ogreid
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Ulvik
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Horn
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Søndenaa
D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality; D000368:Aged; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D011905:Genes, ras; D006801:Humans; D010179:Pancreas / Q000000981:diagnostic imaging / Q000473:pathology; D010189:Pancreatic Juice / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality; D011379:Prognosis; D014057:Tomography, X-Ray Computed
D004273:DNA, Neoplasm
Ki-ras-analyse av pancreasvaeskeaspirat. Ny diagnostisk mulighet ved utredning av pancreascancer.