Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells.
To identify genes mediating programmed cell death triggered by interleukin 3 (IL-3)-deprivation of myeloid cells, the IL-3-dependent murine myeloid cell line FDCP-1 was used to screen a mammalian cell expression library for cDNAs that would promote survival following withdrawal of IL-3. A unique 892-base pair cDNA was cloned that prevented the programmed cell death response following IL-3 deprivation by causing antisense suppression of an endogenous 2.4-kilobase (kb) mRNA. A 2.3-kb cDNA containing the identical 892-base pair over-lapping sequence was cloned that encoded a deduced 371-amino acid protein containing a single Kruppel-type zinc finger and a cluster of 4 cysteine/histidine-rich repeats resembling atypical zinc fingers. The 2.4-kb mRNA was found to be ubiquitously expressed in murine tissues and its abundance in FDCP-1 cells was not altered in response to IL-3 deprivation. Since expression of this 2.4-kb mRNA was a prerequisite for the apoptosis response following IL-3 deprivation, the gene encoding it was named requiem. Requiem is likely to encode a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells.
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202-5121.
T G
TG
Gabig
P L
PL
Mantel
R
R
Rosli
C D
CD
Crean
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D002455:Cell Division; D002478:Cells, Cultured; D018076:DNA, Complementary; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007377:Interleukin-3 / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014162:Transfection; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary; D007377:Interleukin-3