Colorimetric micromethod for protein determination with erythrosin B.
Reaction of erythrosin B with proteins results in a stable, highly colored chromophore with an absorbance maximum at 545 nm. This is the basis for a new quantitative determination method of proteins in solution. The assay can be performed at room temperature, but is faster and more sensitive at 90-95 degrees C. The erythrosin assay is characterized by (i) stable dye-protein color, (ii) high sensitivity (2-14 micrograms/ml protein), (iii) short reaction time (1.5-2 min at 90-95 degrees C), (iv) good reproducibility, (v) limited interference by common reagents, and (vi) low protein-to-protein variability. Thus, the erythrosin assay can be useful for routine analytical purposes and may overcome some of the limitations of other currently employed assays.
Soedjak
H S
HS
Kraft General Foods, Tarrytown, New York 10591.
D016477:Artifacts; D003124:Colorimetry / Q000191:economics / Q000379:methods*; D004923:Erythrosine; D006863:Hydrogen-Ion Concentration; D008846:Micromanipulation / Q000191:economics; D015394:Molecular Structure; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
D011506:Proteins; D004923:Erythrosine