The tumor protein MAGE-1 is located in the cytosol of human melanoma cells.
The MAGE-1 protein has been localized to the cytosol of human melanoma culture cells (MZ2-MEL.43) by subcellular fractionation. Its distribution between nuclear, mitochondrial, microsomal and cytosolic fractions was established by SDS-PAGE and immunoblotting with a rabbit antiserum and compared to that of markers for the main cell compartments. The immunoreactive material was recovered in the cytosolic fraction as a polypeptide migrating at 42 kDa, which has been further identified as the MAGE-1 protein because it comigrates with the product of cell-free transcription-translation of the MAGE-1 cDNA. Location to the cytosol was confirmed by immunofluorescence microscopy.
International Institute of Cellular and Molecular Pathology, Université de Louvain, Brussels, Belgium.
A
A
Amar-Costesec
D
D
Godelaine
E
E
Stockert
P
P
van der Bruggen
H
H
Beaufay
Y T
YT
Chen
D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D018076:DNA, Complementary; D006801:Humans; D008545:Melanoma / Q000378:metabolism*; D058950:Melanoma-Specific Antigens; D008856:Microscopy, Fluorescence; D009363:Neoplasm Proteins; D014176:Protein Biosynthesis; D013347:Subcellular Fractions / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D018076:DNA, Complementary; C072011:MAGEA1 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins