A simple procedure to differentiate ailA and ailNA gene variants among human pathogenic Yersinia enterocolitica strains.
The identification and differentiation of the two variants of the ail gene, ailA from the more virulent American serotypes (08, 013a, 13b, 018, 020, 021) and ailNA from the less virulent non-American serotypes (03, 04, 05, 06, 09, 027 and 07, 8) was studied in a panel of 32 Yersinia enterocolitica human pathogenic isolates. A 444 bp fragment corresponding to the ail gene was amplified using a PCR procedure in all tested strains. Subsequent digestion of the PCR product by Rsal and by HaeIII endonucleases, provide electrophoretic patterns that clearly discriminate ailA and ailNA variants. This non-radioactive and reliable procedure allows large clinical and epidemiological studies, and could be proposed to survey the spread of virulent clones.
Laboratoire de Biochimie Génétique, Hôpital Robert Debré, Paris, France.
C
C
Sayada
B
B
Picard
J
J
Elion
R
R
Krishnamoorthy
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005798:Genes, Bacterial; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015009:Yersinia Infections / Q000175:diagnosis / Q000235:genetics; D015008:Yersinia enterocolitica / Q000145:classification / Q000235:genetics*
D004269:DNA, Bacterial