[The use of a protoplast-generating method in selecting the lytic enzyme producer Streptomyces recifensis subsp. lyticus].
Optimal conditions for the formation of stable protoplasts of S. recifensis subsp. lyticus 2435 were defined and the use of the protoplasting procedure to increase the activity of the synthesis of extracellular enzymes was shown in principle possible. The level of the synthesis of glycosidases and lytic endopeptidases by the isolated variant II-29 was higher than that the initial strain by 40-45 per cent which was due to the enrichment of the population with more active variants.
T Ia
TIa
Kulikova
A S
AS
Sten'ko
N K
NK
Bezkorovaĭnaia
Iu S
IuS
Babenko
D001412:Bacillus subtilis / Q000201:enzymology; D001431:Bacteriological Techniques; D003470:Culture Media; D008837:Micrococcus / Q000201:enzymology; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D011523:Protoplasts / Q000201:enzymology*; D012641:Selection, Genetic; D013211:Staphylococcus aureus / Q000201:enzymology; D013302:Streptomyces / Q000201:enzymology*
D003470:Culture Media; D010447:Peptide Hydrolases
Ispol'zovanie metoda protoplastirovaniia v selektsii produtsenta liticheskikh fermentov Streptomyces recifensis subsp. lyticus.