Analytical bias by contamination from hemolysis in determination of serum lactate dehydrogenase isoenzyme 1 in patients with testis germ cell tumors.
We investigated the impact of correction for contamination from hemolysis on serum lactate dehydrogenase isoenzyme 1 (S-LD-1) determinations. A study of hemolysates from 7 control patients showed a mean correction factor for the contamination of 0.1 U/L S-LD-1 for each 1 mg/L serum hemoglobin (S-Hb). S-LD-1 in a series of blood samples from 44 patients (EJC 1992;28:410-5) would decrease median 24 U/L (range 8-70 U/L) if the measurements were corrected with this factor. So we advice to correct S-LD-1 determinations for the contamination with a common correction based on the S-Hb concentrations in the samples.
von Eyben
F E
FE
Department of Clinical Chemistry, Odense University Hospital, Denmark.
Petersen
P H
PH
Blaabjerg
O
O
Madsen
E L
EL
Nørgaard-Pedersen
B
B
Arends
J
J
D002623:Chemistry Techniques, Analytical / Q000592:standards*; D004340:Drug Contamination; D018237:Germinoma / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008297:Male; D008962:Models, Theoretical; D011785:Quality Assurance, Health Care; D011786:Quality Control; D013736:Testicular Neoplasms / Q000097:blood*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase