EDMA 2000 as a matrix for high-performance liquid chromatography of human haemoglobin chains.
An ethylenedimethacrylate polymer-based matrix (EDMA 2000) is described that shortens the time for separation of haemoglobin (Hb) chains to 30 min, compared with the use of C4 large-pore Vydac columns, commonly used for reversed-phase HPLC of Hb variants. EDMA 2000 was successfully used for the isolation of an abnormal beta-chain of the unstable Hb recently characterized as Hb Nottingham.
Department of Biochemistry, Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
J
J
Suttnar
H
H
Fortová
V
V
Brabec
D002648:Child; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000737:chemistry; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D006418:Heme / Q000302:isolation & purification; D006454:Hemoglobins / Q000302:isolation & purification*; D006455:Hemoglobins, Abnormal / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007223:Infant; D008689:Methacrylates; D013056:Spectrophotometry, Ultraviolet
D006454:Hemoglobins; D006455:Hemoglobins, Abnormal; D007202:Indicators and Reagents; D008689:Methacrylates; D006418:Heme; C075791:hemoglobin Nottingham