The micro-determination of serum gamma-glutamyl transferase.
The method of serum gamma-glutamyl transferase colorimeter assay was modified by changing the wave length from 420 nm to 400 nm, the incubated temperature from 37 degrees C to 50 degrees C, the sample amount from 30 microliters to 6 microliters. The modified method was proved to be more sensitive, with CV of intra-group being 2.06% and accuracy 97.4%. The method is suitable for clinical application with small-amount blood samples collected from earlobe or finger tip.
Department of Otolaryngology, Tongji Hospital, Tongji Medical University, Wuhan.
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Zhen
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Tan
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Cai
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He
D000328:Adult; D003124:Colorimetry / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D012016:Reference Values; D012680:Sensitivity and Specificity; D005723:gamma-Glutamyltransferase / Q000097:blood*
D005723:gamma-Glutamyltransferase