Association of p53 expression with cytokinetics and HPV capsid antigen prevalence in oral carcinomas.
p53 protein expression in oral squamous cell carcinomas (SCCs) was studied in relation to presence of HPV capsid antigens and tumour cell kinetics. The protein and viral antigens were determined from 64 SCCs employing immunoperoxidase and immunofluorescence techniques, respectively. Cell kinetics were assessed by in vitro bromodeoxyuridine labelling. Different levels of p53 were detected in 61% cases. Well-differentiated SCCs (88%) showed increased p53 expression compared with the less differentiated ones. In SCCs with higher cell proliferation rate (LI%, 13.25 +/- 4.64) the expression was almost absent. However, it was higher in stage I than in stage II/III carcinomas and occurred in 54.5% and 75% cases of HPV antigen-positive and -negative (or very little) SCCs, respectively.
Department of Tumour Virology, Chittaranjan National Cancer Institute, Calcutta, India.
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Mukhopadhyay
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Chatterjee
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Chakraborty
D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000032:analysis; D002213:Capsid / Q000032:analysis / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000821:virology; D002455:Cell Division; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000276:immunology / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics; D014412:Tumor Virus Infections / Q000150:complications
D000956:Antigens, Viral; D016159:Tumor Suppressor Protein p53