Immune responses and protective effect in mice vaccinated orally with surface sporozoite protein of Eimeria falciformis in ISCOMs.
Immunostimulating complexes (ISCOMs) were built after treatment of a purified surface protein from Eimeria falciformis sporozoites with a palmitic acid derivation, leading to a high ratio (33-64%) of P27 incorporation in these cage-like structures. P27 kept its antigenicity after incorporation in ISCOMs, which induced, after iterative intubations by the oral route to groups of mice, a systemic IgG response, a local IgA response, and a local enhanced cellular response as demonstrated by lymphoproliferation of mesenteric lymph node cells upon in vitro stimulation with antigen. This immunization (120 micrograms in six oral doses at 2-day intervals) afforded mice a partial protection (60%) against a subsequent 400 oocyst challenge. The reduction in daily oocyst excretion was corroborated by significantly different weight losses between immunized and control mice on days 9 and 10 postinfection and the subsequent death of these control mice. These observations provide the first application of ISCOMs to parasitic intestinal diseases.
INRA, Unité de Virologie et Immunologie Moléculaires, Jouy-en-Josas, France.
M
M
Kazanji
F
F
Laurent
P
P
Péry
D000284:Administration, Oral; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001774:Blood Chemical Analysis; D015153:Blotting, Western; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D003048:Coccidiosis / Q000517:prevention & control*; D004539:Eimeria / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016882:ISCOMs / Q000008:administration & dosage / Q000276:immunology* / Q000627:therapeutic use / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010168:Palmitates / Q000378:metabolism; D015800:Protozoan Proteins / Q000276:immunology* / Q000378:metabolism; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016882:ISCOMs; D008565:Membrane Proteins; D010168:Palmitates; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D014613:Vaccines, Attenuated