Removal of beta 2-microglobulin using grafted affinity adsorbents as therapeutic approach for the treatment of hemodialysis patients.
Patients undergoing long-term hemodialysis are increasingly faced with the problem of developing amyloidosis. This is particularly manifested in a deposition of amyloid in diverse tissues and joints. For the treatment and prevention of amyloidosis, novel affinity adsorbents, which were prepared on the basis of radiation grafted polyamide, have been developed to remove beta 2-microglobulin (beta 2-MG) which is the main constituent of the amyloid. Various affinity ligands, such as alkyl residues with different chain lengths, as well as collagen and gelatin were tested for removing beta 2-MG from human serum and hemofiltrates (HF). Collagen and gelatin carriers show the best adsorption performances. They remove > 95% and > 50% of the beta 2-MG from HF and serum, respectively. The results show that the present approach can be used as the basis for future development of beta 2-MG adsorbents as an additional means in the treatment of dialysis patients.
Institut für Anorganische Chemie, RWTH Aachen, Germany.
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D
Müller-Schulte
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H
Melzer
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H
Mann
D000327:Adsorption; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D006435:Renal Dialysis / Q000295:instrumentation*; D016622:Silver Staining; D001613:beta 2-Microglobulin / Q000737:chemistry / Q000302:isolation & purification*
D001613:beta 2-Microglobulin