Evaluation of a novel diagnostic test for canine parvovirus.
The CPV ELISA detection kit (CSL Ltd., Melbourne Australia) was developed as a rapid field test for the detection of canine parvovirus (CPV) antigen in canine faecal samples. The kit utilises a novel concept in enzyme immunoassay (ELISA) where the conjugate is lyophilised along with the solid-phase capture antibody in the test well. The CPV ELISA was compared to the haemagglutination assay (HA) test using electron microscopy (EM) and/or virus isolation (VI) to confirm infection. The CPV ELISA had a sensitivity of 87% and a specificity of 100% compared to 87% and 63% respectively for the HA. The poor specificity of the HA results in a low positive predictive value of 51% compared to 100% for the CPV ELISA. The CPV ELISA requires no specialised equipment, is simple to perform and provides a visible result in less than 15 minutes.
Drane
D P
DP
Research and Development Division, CSL Limited, Parkville, Vic., Australia.
Hamilton
R C
RC
Cox
J C
JC
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D006386:Hemagglutination Tests / Q000662:veterinary; D008854:Microscopy, Electron / Q000662:veterinary; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors
D000956:Antigens, Viral