Involvement of phosphatidylinositol synthesis in the regulation of S phase induction.
The addition of serum to quiescent normal rat kidney (NRK) cells induced phosphatidylinositol (PI) synthesis after 4 h and DNA synthesis after 16 h. Inostamycin, an inhibitor of CDP-DG:inositol transferase, added within 4 h, inhibited both the serum-induced PI synthesis and S phase entry. By contrast, inostamycin added 8 h after release from quiescence did not inhibit the S phase entry. Inostamycin did not affect the G2/M/G1 transition, and incubation with inostamycin induced cell synchronization at early G1 phase. Thus, PI synthesis is involved in the regulation of S phase induction.
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
M
M
Imoto
T
T
Morii
A
A
Deguchi
K
K
Umezawa
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001839:Bombesin / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005663:Furans / Q000302:isolation & purification / Q000494:pharmacology; D006706:Homeostasis; D007295:Inositol Phosphates / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D007668:Kidney; D007700:Kinetics; D010716:Phosphatidylinositols / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D051381:Rats; D016196:S Phase / Q000187:drug effects / Q000502:physiology*; D013302:Streptomyces; D013997:Time Factors
D000900:Anti-Bacterial Agents; D005663:Furans; D007295:Inositol Phosphates; D007328:Insulin; D010716:Phosphatidylinositols; D010982:Platelet-Derived Growth Factor; C071861:inostamycin; D004815:Epidermal Growth Factor; D004247:DNA; D001839:Bombesin