Identification of genes involved in DNA replication of the Autographa californica baculovirus.
By use of a transient replication assay, nine genes involved in DNA replication were identified in the genome of the Autographa californica baculovirus. Six genes encoding helicase, DNA polymerase, IE-1, LEF-1, LEF-2, and LEF-3 are essential for DNA replication while three genes encoding P35, IE-2, and PE38 stimulate DNA replication. No stimulation by the AcMNPV pcna gene, encoding a protein with sequence homology to proliferating-cell nuclear antigen, was observed. A pattern of amino acids found in a number of single-stranded-DNA-binding proteins was identified in the carboxyl-terminal region of IE-1.
Department of Virology, Wageningen Agricultural University, The Netherlands.
M
M
Kool
C H
CH
Ahrens
R W
RW
Goldbach
G F
GF
Rohrmann
J M
JM
Vlak
D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D015183:Restriction Mapping; D015678:Viral Structural Proteins / Q000235:genetics*; D014779:Virus Replication
D004279:DNA, Viral; D015678:Viral Structural Proteins