Fibronectin-induced cell spreading and down-regulation of 12-lipoxygenase expression in megakaryocytic DAMI cells.
During maturation of small immature megakaryocytes to mature platelet producing megakaryocytes the arachidonic acid metabolizing enzyme 12-lipoxygenase is expressed. Here we show that cells of the megakaryocytic cell line DAMI flatten and spread on fibronectin substrate in a way which may be similar to the spreading observed with bone marrow-derived megakaryocytes. The effect on DAMI cell morphology may be attributed to the RGD motif (Arg-Gly-Asp) of fibronectin. Incubation of DAMI cells with fibronectin down-regulated 12-lipoxygenase expression about 85-90%. We discuss the hypothesis that 12-HETE in megakaryocytes may act as a sensor for megakaryocyte attachment to matrix components.
Kuchinke
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Department of Pharmacology, Vanderbilt University, Nashville, TN 37232.
Funk
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D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects*; D005353:Fibronectins / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology / Q000187:drug effects*
D005353:Fibronectins; D001092:Arachidonate 12-Lipoxygenase