ATPase activity of melanosomes in liver pigment cells of Rana esculenta L.
Luminometric methods show that melanosomes in liver pigment cells of Rana esculenta L. have endogenous ATP and ATPase activity. The Km value of ATPase is 0.42 x 10(-8) mol/l at pH 7.0. Inhibition of ATPase by antimycin and by ouabain is not effective. In the presence of an excess of ADP and Pi, ATP synthesis was observed.
Sichel
G
G
Istituto di Biologia Generale, University of Catania, Italy.
Corsaro
C
C
Cassiani
E
E
Magnani
C
C
Bolognani
L
L
D000251:Adenosine Triphosphatases / Q000096:biosynthesis*; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives / Q000494:pharmacology; D005456:Fluorescent Dyes; D008099:Liver / Q000201:enzymology*; D008544:Melanocytes / Q000201:enzymology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010042:Ouabain / Q000494:pharmacology; D011893:Rana esculenta
D005456:Fluorescent Dyes; C032456:antimycin; D010042:Ouabain; D000968:Antimycin A; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases