Antigenic outline of HHV-6 variants from infants with febrile illness.
The surface antigenic make-up of 10 HHV-6 isolates from cases of infantile disease was analyzed by immunofluorescence assay with 5 monoclonal antibodies (Mabs). Three Mabs were directed to glycoproteins (gps) expressed in cells infected with the HHV-6 variant A prototype GS, and 2 to gps expressed in cells infected with the HHV-6 variant B prototype Z29. Of the 10 viral isolates, all belonging to HHV-6 variant B, 9 showed a similar Mab reactivity, while 1, from a case of gastroenteric illness, differed widely from the others. The HHV-6 isolates studied also differed from the variant B prototype Z29 for the absence of reactivity to one of the Mabs. The choice of the HHV-6 new isolates as variant B prototypes is recommended.
Institute of Hygiene, University of Modena, Italy.
C
C
Cermelli
G
G
Fabio
M
M
Montorsi
D
D
Di Luca
M
M
Portolani
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D005334:Fever / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D014644:Genetic Variation; D006566:Herpesviridae Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007223:Infant
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral