A new hybrid promoter directs transcription at identical start points in mammalian cells and in vitro.
A hybrid promoter which generates large amounts of mRNAs with transcription start points (tsp) differing in one nt, both in mammalian cells and in vitro, was constructed by integrating the promoter of bacteriophage T7 in between the TATA box and the tsp of the retroviral myeloproliferative sarcoma virus (MPSV) long terminal repeat (LTR). This promoter was designed for sequence and secondary structure studies of 5' untranslated regions (UTR) with respect to mRNA stability and translatability.
Dirks
W
W
Department of Human and Animal Cell Culture, DSM-German Collection of Microorganisms and Cell Cultures, Braunschweig.
Schaper
F
F
Hauser
H
H
D000818:Animals; D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D015870:Gene Expression; D007668:Kidney; D008322:Mammals; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics; D016385:TATA Box; D014158:Transcription, Genetic; D014162:Transfection / Q000379:methods*
D012333:RNA, Messenger