Chemotaxis of freshly separated and cultured human keratinocytes.
It is generally accepted that keratinocyte migration plays a critical role in the process of wound healing. A study was therefore made of the migratory response of freshly separated and cultured human keratinocytes to factors with chemotactic properties for a variety of cells. Interleukin-1 alpha (IL-1 alpha), interferon-gamma (IFN-gamma), interleukin-8 (IL-8), and tumour necrosis factor alpha (TNF-alpha) were tested for their chemotactic effectiveness in a modified Boyden chamber assay. IFN-gamma, IL-1 alpha and IL-8 were demonstrated to serve as chemoattractants for freshly separated keratinocytes. For cultured cells, however, only IFN-gamma was found to display chemotactic properties. The findings demonstrate that there is significant difference between the chemotactic behaviour of freshly separated and cultured cells.
Department of Dermatology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
R
R
Gyulai
J
J
Hunyadi
A
A
Kenderessy-Szabó
L
L
Kemény
A
A
Dobozy
D002478:Cells, Cultured; D002633:Chemotaxis; D016207:Cytokines / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology; D015603:Keratinocytes / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016207:Cytokines; D007375:Interleukin-1; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma