Synthesis of peptides mediated by KOBt.
Coupling of Fmoc-amino acid chlorides can be mediated by the potassium salt of 1-hydroxybenzotriazole (KOBt), the reaction being carried out in an organic medium. The use of a base like NaHCO3/Na2CO3 or DIEA/NMM/pyridine is not necessary. Coupling is fast and racemization free; the work-up, isolation of the product and scale-up are easy. The pentapeptide sequence of Fmoc-[Leu]enkephalin was thus synthesized in the solution phase on a 5 mmol scale without isolation of any intermediate. Acylation of C-protected N-methylamino acid esters by Fmoc-N-methylamino acid chlorides by this procedure is also feasible, as demonstrated by the synthesis of cyclosporin A fragments 4-7 and 8-11. The peptides obtained in high yields were crystalline solids, unlike earlier reports in which they were obtained mostly as oily or foamy intermediates. They showed spectral properties identical with those of the authentic compounds.
Department of Studies in Chemistry, Central College, Bangalore University, Karnataka, India.
K M
KM
Sivanandaiah
V V
VV
Babu
S C
SC
Shankaramma
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000138:chemical synthesis; D016572:Cyclosporine / Q000138:chemical synthesis*; D004745:Enkephalins / Q000138:chemical synthesis*; D005449:Fluorenes / Q000138:chemical synthesis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis*; D017680:Potassium Compounds / Q000737:chemistry*; D014230:Triazoles / Q000737:chemistry*
D000596:Amino Acids; D004745:Enkephalins; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010446:Peptide Fragments; D017680:Potassium Compounds; D014230:Triazoles; D016572:Cyclosporine; C011852:1-hydroxybenzotriazole