Establishment of a partially informative porcine somatic cell hybrid panel and assignment of the loci for transition protein 2 (TNP2) and protamine 1 (PRM1) to chromosome 3 and polyubiquitin (UBC) to chromosome 14.
Porcine lymphocytes and fibroblasts were fused with 3 different permanent rodent cell lines, and 21 stable somatic cell hybrid lines were established. These hybrid cell lines were characterized cytogenetically by sequential QFQ banding and chromosome painting using fluorescence in situ hybridization with porcine DNA. The lines were further characterized by PCR analysis with primer pairs derived from genes with confirmed mapping information. Using this panel, we assigned the locus encoding polyubiquitin (UBC) to chromosome 14, and the transition protein 2 locus (TNP2) and protamine loci (PRM1 and PRM2) to chromosome 3. Two chromosomal localizations have been further refined by radioactive in situ hybridization. UBC maps to chromosome 14q12-q15 and TNP2 to 3p11-p12.
Rettenberger
G
G
Abteilung Klinische Genetik, Universität Ulm, Germany.
Fries
R
R
Engel
W
W
Scheit
K H
KH
Dolf
G
G
Hameister
H
H
D000818:Animals; D001483:Base Sequence; D001704:Biopolymers / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D006224:Cricetinae; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005347:Fibroblasts / Q000378:metabolism; D006822:Hybrid Cells; D017403:In Situ Hybridization; D007621:Karyotyping; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D025821:Polyubiquitin; D011479:Protamines / Q000235:genetics*; D015183:Restriction Mapping; D013552:Swine / Q000235:genetics*; D014452:Ubiquitins / Q000235:genetics*
D001704:Biopolymers; D017931:DNA Primers; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C513176:Prm1 protein, mouse; D011479:Protamines; C489505:Tnp2 protein, mouse; D014452:Ubiquitins; D025821:Polyubiquitin