Sequence of the cloned bseCIM gene: M.BseCI reveals high homology to M.BanIII.
The bseCIM gene, encoding M.BseCI methyltransferase (MTase) from a Bacillus stearothermophilus strain, has been previously cloned and expressed in Escherichia coli [Rina and Bouriotis, Gene 133 (1993) 91-94]. The nucleotide (nt) sequence of a 2357-bp BspMII-EcoRI fragment encoding bseCIM has now been determined. The sequence predicts a MTase of 579 amino acids (aa), 66.7 kDa. Comparison of the deduced aa sequence of M.BseCI with sequences of various MTases revealed a significant homology to m6A-MTases, especially to its isoschizomer M.BanIII from Bacillus aneurinolyticus.
Institute of Molecular Biology and Biotechnology, Enzyme Technology Division, Crete, Greece.
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Rina
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Markaki
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Bouriotis
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics*
C061926:DNA modification methylase Cla I; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)