Reactive blue 2 discriminates between responses mediated by UTP and those evoked by ATP or alpha,beta-methylene-ATP on rat sympathetic ganglia.
The effect of reactive blue 2, a P2 purinoceptor antagonist on depolarisation of the rat superior cervical ganglion evoked by ATP, alpha,beta-methylene-ATP (alpha,beta-Me-ATP) and uridine 5'-triphosphate (UTP) was determined. UTP, alpha,beta-Me-ATP and ATP (in the presence of an A1 purinoceptor antagonist) produced a depolarisation in that order of potency. Reactive blue 2 (30 microM) antagonised alpha,beta-Me-ATP-evoked depolarisations without altering responses evoked by adenosine or UTP. Reactive blue (100 or 300 microM) enhanced UTP- and depressed alpha,beta-Me-ATP- or ATP- (at 100 microM reactive blue 2) evoked depolarisations. These results indicate that rat superior cervical ganglia possess purinoceptors and pyrimidinoceptors.
Department of Physiology, University College London, UK.
G P
GP
Connolly
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PJ
Harrison
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005728:Ganglia, Sympathetic / Q000187:drug effects* / Q000502:physiology; D008297:Male; D058919:Purinergic P2 Receptor Antagonists; D051381:Rats; D017207:Rats, Sprague-Dawley; D014227:Triazines / Q000494:pharmacology*; D014544:Uridine Triphosphate / Q000494:pharmacology*
D058919:Purinergic P2 Receptor Antagonists; D014227:Triazines; C012842:Cibacron Blue F 3GA; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D014544:Uridine Triphosphate