Similarities and differences between fish oligodendrocytes and Schwann cells in vitro.
In light of the striking differences between oligodendrocytes of the optic nerve/tract of adult goldfish and their mammalian counterparts, a further characterization of goldfish oligodendrocytes was performed. A comparison with Schwann cells was included because fish optic nerve/tract-derived oligodendrocytes bear remarkable similarities to this type of glial cell. Fish optic nerve/tract-derived oligodendrocytes that had differentiated into 04 and 6D2-positive cells and thus expressed early myelin marker molecules were found to incorporate BrdU and to divide in vitro over a period of weeks. For the induction of more advanced markers of myelinogenesis such as the CNS-specific myelin protein 36K, oligodendrocytes required extensive contact with axons. Other agents, such as fetal calf or carp serum, substrate components, or forscolin failed, however, to induce 36K expression. 04/6D2-positive oligodendrocytes could be distinguished from fish 6D2-positive Schwann cells derived from cranial nerves by their antigenic phenotype: Schwann cells but not oligodendrocytes exhibited the low affinity NGF receptor. While both cell types carry the cell adhesion molecules NCAM, E 587 antigen, and the L2/HNK-1 epitope, only Schwann cells possess a further adhesion molecule, Neurolin.
Faculty of Biology, University of Konstanz, Germany.
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Bastmeyer
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Jeserich
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Stuermer
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D006054:Goldfish / Q000502:physiology*; D007150:Immunohistochemistry; D009185:Myelin Proteins / Q000096:biosynthesis; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009836:Oligodendroglia / Q000378:metabolism / Q000502:physiology*; D009900:Optic Nerve / Q000166:cytology; D017475:Receptors, Nerve Growth Factor / Q000187:drug effects / Q000378:metabolism; D012583:Schwann Cells / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D009185:Myelin Proteins; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor; D005576:Colforsin; D001973:Bromodeoxyuridine