Synaptotagmin I is essential for Ca(2+)-independent release of neurotransmitter induced by alpha-latrotoxin.
alpha-Latrotoxin causes massive release of norepinephrine from clonal rat pheochromocytoma PC12 cells, in the absence of external Ca2+, by an unknown mechanism. The effect almost disappeared in PC12 variant cells deficient in synaptotagmin I, a synaptic vesicle protein, and was rescued by transfecting the synaptotagmin I gene. These results indicate that synaptotagmin I is essential for the Ca(2+)-independent action of alpha-latrotoxin in PC12 cells.
Shoji-Kasai
Y
Y
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Yoshida
A
A
Ogura
A
A
Kuwahara
R
R
Grasso
A
A
Takahashi
M
M
D000818:Animals; D002118:Calcium / Q000502:physiology; D002135:Calcium-Binding Proteins; D003470:Culture Media; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009638:Norepinephrine / Q000378:metabolism*; D016716:PC12 Cells; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D013111:Spider Venoms / Q000494:pharmacology*; D050863:Synaptotagmin I; D050857:Synaptotagmins; D014162:Transfection
D002135:Calcium-Binding Proteins; D003470:Culture Media; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D013111:Spider Venoms; D050863:Synaptotagmin I; C494312:Syt1 protein, rat; D050857:Synaptotagmins; C020763:alpha-latrotoxin; D011188:Potassium; D002118:Calcium; D009638:Norepinephrine