A multifunctional vector family for gene expression in mammalian cells.
A family of different promoters and vector backbones for multiple use in mammalian cells in vivo and in vitro experiments has been created. The elements allow high-level constitutive, as well as adjustable expression with mono- and dicistronic transcription units and several in vitro manipulations of the inserted ORFs. The vector components are designed to be combined in a simple cloning step.
Dirks
W
W
Department of Human and Animal Cell Culture, DSM--German Collection of Microorganisms and Cell Cultures, Braunschweig.
Schaper
F
F
Kirchhoff
S
S
Morelle
C
C
Hauser
H
H
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D015870:Gene Expression; D005796:Genes; D005822:Genetic Vectors; D008322:Mammals; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D014158:Transcription, Genetic; D014162:Transfection / Q000379:methods*; D014982:Xenopus laevis