Splenic interleukin 1 gene expression is associated with accumulation of macrophages and oxygen radical production in Plasmodium vinckei malaria.
High levels of interleukin 1 alpha (IL-1 alpha) were detected in vitro, in murine peritoneal macrophages stimulated with Plasmodium vinckei exogenous antigens, and in vivo, in sera of P. vinckei-parasitized mice. Moreover, high production of IL-1 alpha mRNA could be detected by in situ hybridization analysis in spleen sections of mice during the course of P. vinckei malaria. The observed IL-1 alpha gene expression in the spleen was associated with the accumulation of F4/80+ macrophages in the red pulp and in the marginal zone of follicles, as well as with the relative proportions of Mac-1+ cells in the spleen and the capacity of spleen cells to produce reactive oxygen intermediates during murine malaria.
Landesinstitut für Tropenmedizin Berlin, Germany.
J
J
Prada
S
S
Neifer
S
S
Müller
R
R
Rudolph
U
U
Bienzle
P G
PG
Kremsner
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008264:Macrophages / Q000276:immunology*; D008288:Malaria / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010961:Plasmodium / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D013154:Spleen / Q000276:immunology*
D000953:Antigens, Protozoan; D007375:Interleukin-1; D012333:RNA, Messenger; D006861:Hydrogen Peroxide