Molecular cloning of the B-CAM cell surface glycoprotein of epithelial cancers: a novel member of the immunoglobulin superfamily.
The human F8/G253 antigen, B-CAM, is a cell surface glycoprotein that is expressed with restricted distribution pattern in normal fetal and adult tissues, and is up-regulated following malignant transformation in some cell types. We have isolated a complementary DNA for B-CAM using an expression cloning technique. The complementary DNA (EMBL accession number X80026) encodes a 588-amino acid protein which is a novel member of the immunoglobulin superfamily with a characteristic V-V-C2-C2-C2 immunoglobulin domain structure. This structure has been described previously for the human MUC18 melanoma antigen (31% amino acid identity) and chicken and rat versions of a neural adhesion molecule referred to as SC1/DM-GRASP/BEN or KG-CAM, respectively (26% amino acid identity). This homology is suggestive of a role for B-CAM in cell-cell or cell-matrix adhesion. The gene for B-CAM has been mapped by fluorescence in situ hybridization to chromosome 19q13.2-13.3.
University of Southampton, Princess Anne Hospital, United Kingdom.
I G
IG
Campbell
W D
WD
Foulkes
G
G
Senger
J
J
Trowsdale
P
P
Garin-Chesa
W J
WJ
Rettig
D020410:Activated-Leukocyte Cell Adhesion Molecule; D000595:Amino Acid Sequence; D015703:Antigens, CD; D001483:Base Sequence; D015152:Blotting, Northern; D051929:CD146 Antigen; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics*; D002888:Chromosomes, Human, Pair 19 / Q000737:chemistry; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008188:Lutheran Blood-Group System; D008562:Membrane Glycoproteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics; D019006:Neural Cell Adhesion Molecules; D014407:Tumor Cells, Cultured
D020410:Activated-Leukocyte Cell Adhesion Molecule; D015703:Antigens, CD; C090228:BCAM protein, human; D051929:CD146 Antigen; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D008188:Lutheran Blood-Group System; C497902:MCAM protein, human; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules