High-performance liquid chromatographic determination of norepinephrine, epinephrine and dopamine in human foetal adrenal gland.
The purpose of this work was to characterize the human foetal adrenal gland (HFAG) by studying norepinephrine (NE), epinephrine (E), and dopamine (DA) levels in foetuses with ages ranging from 10 to 18 weeks, as a source of chromaffin cells for basic and clinical research, in the treatment of Parkinson's disease. The HFAGs were obtained from ten abortions with foetal ages ranging from 10-12 (n = 7) to 13-18 (n = 3) weeks. For the simultaneous detection of NE, E, and DA a high-performance liquid chromatographic (HPLC) procedure with electrochemical detection was employed. The concentrations found (ng/mg wet weight) were 24.24 +/- 7.06 for NE, 3.56 +/- 2.97 for E and 0.45 +/- 0.27 for DA at 10-12 weeks and 14.91 +/- 9.94 for NE, 8.18 +/- 8.79 for E and 0.16 +/- 0.05 for DA at 13-18 weeks. The DA:E ratio present in HFAG between 10 and 12 weeks was 100 times higher than that reported by other authors in adult adrenal medulla.
Centro Internacional de Restauracion Neurologica, Habana, Cuba.
J C
JC
Garciá
L
L
Blanco
M
M
McPherson
A
A
Leiva
R
R
Maciás
D000311:Adrenal Glands / Q000737:chemistry* / Q000196:embryology; D002395:Catecholamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000032:analysis; D004563:Electrochemistry; D004837:Epinephrine / Q000032:analysis; D005260:Female; D005865:Gestational Age; D006801:Humans; D009638:Norepinephrine / Q000032:analysis; D011247:Pregnancy
D002395:Catecholamines; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine