[The creation of diet-dependent cancer models using transgenesis in animals].
We have created transgenic mice lines in which SV40 T and c-myc expression was controlled by the L-pyruvate kinase gene regulatory region which is responsible for hepatic and pancreatic expression specificity, and strong dependence of this expression upon the carbohydrate composition of the diet. Models of hepatoma and endocrine pancreatic tumors have been obtained. Both tumors were dependent upon the diet, since carbohydrates strongly increased frequency and precocity of both hepatic and pancreatic carcinomas.
Institut Cochin de Génétique moléculaire, U 129 Inserm, Paris.
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N
Cartier
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P
Briand
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A
Kahn
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D004032:Diet / Q000009:adverse effects*; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000009:adverse effects; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc / Q000235:genetics; D006965:Hyperplasia; D008099:Liver / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000209:etiology* / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D009857:Oncogenes / Q000235:genetics*; D010179:Pancreas / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000209:etiology* / Q000235:genetics*; D011120:Polyomavirus / Q000276:immunology; D011770:Pyruvate Kinase / Q000235:genetics; D013395:Sucrose / Q000008:administration & dosage / Q000009:adverse effects
D000952:Antigens, Polyomavirus Transforming; D004040:Dietary Carbohydrates; D013395:Sucrose; D011770:Pyruvate Kinase
Création de modèles de cancers dépendant du régime alimentaire par transgenèse chez l'animal.