Safety evaluations of food chemicals by "COMPACT". 1. A study of some acyclic terpenes.
A group of 19 acyclic terpenes have been evaluated for potential toxicity/carcinogenicity by molecular orbital determinations of their spatial and electronic parameters, and hence prediction of their metabolic activation or detoxication by the cytochrome P-450 (CYP) superfamily of mixed-function oxidase enzymes. Previous studies have characterized the spatial dimensions of the CYP1A1, 1A2 and 2E1 enzymes, which are known to activate mutagens and carcinogens and to be involved in other mechanisms of toxicity. None of the terpenes was found to have shape or electronic parameters appropriate for metabolic activation by CYP1A1 or 1A2, and hence they are unlikely to be carcinogenic or mutagenic. Furthermore, none of these chemicals had spatial parameters critical for substrates of CYP2E, and they are therefore unlikely to induce the formation of reactive oxygen species (ROS) or to initiate or promote malignancy or toxicity by mechanisms involving ROS. However, citral, and others of these terpenes, are known to undergo metabolism to carboxylic acids that may induce CYP4, and are therefore possible inducers of hepatic peroxisomal proliferation at high dosage, which may have implications for possible hepatotoxicity.
Lewis
D F
DF
School of Biological Sciences, University of Surrey, Guildford, UK.
Ioannides
C
C
Walker
R
R
Parke
D V
DV
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D003201:Computers; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005503:Food Additives / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D017382:Reactive Oxygen Species; D012449:Safety; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013729:Terpenes / Q000633:toxicity*
D002273:Carcinogens; D005503:Food Additives; D017382:Reactive Oxygen Species; D013729:Terpenes; D003577:Cytochrome P-450 Enzyme System