T cell cytotoxicity of autologous and allogeneic lymphocytes in a patient with Crohn's disease.
We report a 27-year-old male with Crohn's disease (CD) of the small and large intestine, whose peripheral blood lymphocytes (PBL) showed increased cell-mediated cytotoxicity (CTL). Autologous and allogeneic effector cells from PBL and intestinal lymph nodes (LN) were isolated on a Ficoll-Hypaque gradient. Colonic cells were prepared as the target and were incubated for 6h with effector cells, after being labeled with Na(2)51CrO4. The CTL activity [effector/target (E/T) ratio, 100:1] of PBL for autologous targets was increased by 38% compared with that in normal subjects (< 10%), while that shown by LN was not increased (14%). The CTL activity of allogeneic PBL prepared from three of four other CD patients was also increased. Anti-major histocompatibility (MHC) class I and II and CD4 and CD8 monoclonal antibodies (50 micrograms/ml) significantly inhibited CTL activity. Complement-mediated depletion of CD2+ cells significantly reduced CTL activity. These results suggest that MHC-restricted CTL may play a role in mucosal damage in some patients with Crohn's disease.
First Department of Internal Medicine, Kochi Medical School, Japan.
K
K
Okazaki
Y
Y
Yokoyama
Y
Y
Yamamoto
M
M
Kobayashi
K
K
Araki
T
T
Ogata
D000328:Adult; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018801:CD2 Antigens / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002478:Cells, Cultured; D016009:Chi-Square Distribution; D003106:Colon / Q000276:immunology / Q000473:pathology; D003424:Crohn Disease / Q000276:immunology* / Q000473:pathology; D003602:Cytotoxicity, Immunologic; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology; D008212:Lymphocyte Depletion; D008297:Male; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018801:CD2 Antigens; D015704:CD4 Antigens; D016827:CD8 Antigens; D006680:HLA Antigens