Mucosal immunization with a live, virulence-attenuated simian immunodeficiency virus (SIV) vaccine elicits antiviral cytotoxic T lymphocytes and antibodies in rhesus macaques.
An effective AIDS vaccine must protect against sexual transmission of human immunodeficiency virus (HIV). Therefore, vaccine regimens which stimulate antiviral immunity in the genital tract as well as in peripheral blood and systemic lymphoid tissues are needed. Here, we describe a method of immunization by direct inoculation of the vaginal submucosa with a live attenuated SIV, SIVmac1A11. Immunization by this route generated low levels of SIV-specific IgG and IgA antibodies in serum and vaginal secretions and viral specific cytotoxic T lymphocyte (CTL) activity in peripheral blood.
Lohman
B L
BL
California Regional Primate Research Center, University of California, Davis 95616.
McChesney
M B
MB
Miller
C J
CJ
Otsyula
M
M
Berardi
C J
CJ
Marthas
M L
ML
D016915:AIDS Vaccines; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control; D000282:Administration, Intravaginal; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000097:blood; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008214:Lymphocytes / Q000821:virology; D008253:Macaca mulatta; D009092:Mucous Membrane / Q000276:immunology / Q000821:virology; D018449:SAIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology*; D014621:Vagina / Q000276:immunology / Q000821:virology*
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D018449:SAIDS Vaccines; D014613:Vaccines, Attenuated