Cloning and expression of a cDNA for rat prostaglandin F2 alpha receptor.
We have cloned a cDNA for rat prostaglandin (PG) F2 alpha receptor from cultured rat astrocytes. The cDNA encodes a polypeptide of 366 amino acids with seven putative transmembrane domains. Specific binding of [3H]PGF2 alpha in membranes of COS-7 cells transfected with the cDNA was displaced with unlabeled PGs in the order of PGF2 alpha > PGD2 > PGE2 > PGI2. In the cDNA-transfected LLC-PK1 cells, PGF2 alpha stimulated phosphoinositide hydrolysis. A significant 4.7-kb mRNA transcript was detected in cultured rat astrocytes and whole brain and pregnant ovary of adult rats by Northern blot analysis.
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
J
J
Kitanaka
H
H
Hasimoto
Y
Y
Sugimoto
M
M
Negishi
H
H
Aino
M
M
Gotoh
A
A
Ichikawa
A
A
Baba
D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D001483:Base Sequence; D001667:Binding, Competitive; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015237:Dinoprost / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008969:Molecular Sequence Data; D010716:Phosphatidylinositols / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011982:Receptors, Prostaglandin / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D010738:Type C Phospholipases / Q000378:metabolism
D018076:DNA, Complementary; D010716:Phosphatidylinositols; D011453:Prostaglandins; D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; D011994:Recombinant Proteins; C034671:prostaglandin F2alpha receptor; D015237:Dinoprost; D010738:Type C Phospholipases