In situ hybridization study of neuropeptide Y neurons in the rat brain and pelvic paracervical ganglion.
The distribution of mRNA encoding neuropeptide Y in cells of the rat pelvic paracervical ganglion was studied by the use of in situ hybridization with a neuropeptide Y oligodeoxynucleotide probe. The specificity of the hybridization signal was assessed on brain sections and the neurons expressing neuropeptide Y mRNA were actually found in several discrete brain regions already described for containing neuropeptide Y neurons. The present study reports the first demonstration of the presence of neuropeptide Y transcripts in nerve cell bodies and small intensively fluorescent (SIF)-like cells of the pelvic paracervical ganglion, thus providing evidence that neuropeptide Y is synthesized in these two cell types. In addition, a quantitative analysis shows a differential pattern of expression of the peptide mRNA in nerve cell bodies throughout the ganglion.
Université Pierre et Marie Curie, Laboratoire de Physiologie de la Reproduction, CNRS (URA 1449), France.
E
E
Houdeau
P A
PA
Boyer
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000648:ultrastructure; D001923:Brain Chemistry; D004027:Diencephalon / Q000737:chemistry / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D005728:Ganglia, Sympathetic / Q000737:chemistry* / Q000648:ultrastructure; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009474:Neurons / Q000737:chemistry* / Q000648:ultrastructure; D009478:Neuropeptide Y / Q000032:analysis* / Q000235:genetics; D010388:Pelvis / Q000294:innervation*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013687:Telencephalon / Q000737:chemistry / Q000648:ultrastructure
D009478:Neuropeptide Y; D012333:RNA, Messenger