Direct resolution of epoxyeicosatrienoic acid enantiomers by chiral-phase high-performance liquid chromatography.
A chromatographic method was developed to separate enantiomers of four regioisomeric epoxyeicosatrienoic acids (EETs), which are cytochrome P-450 monooxygenase products of arachidonic acid. Enantiomers of individual epoxyeicosatrienoic acids were separated by chiral-phase HPLC using Chiralcel OD columns. In contrast to the pre-derivatization of EETs, the method is simple and convenient. It can be readily used in analytical studies and in preparative applications. The method can also be applied to the separation of methyl ester derivatives of epoxyeicosatrienoic acid enantiomers.
Department of Pharmacology, Vanderbilt University, Nashville, TN 37232-6602.
J Y
JY
Zhang
I A
IA
Blair
D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D016718:Arachidonic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D010105:Oxygenases / Q000378:metabolism; D013237:Stereoisomerism
D016718:Arachidonic Acid; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D015126:8,11,14-Eicosatrienoic Acid