N-terminal domain of dystrophin.
Contro-versial experiments have been published on calmodulin binding of dystrophin. In this study, we used recombinant proteins and the techniques of affinity chromatography and ELISA to show that the N-terminal part of dystrophin binds calmodulin specifically in a calcium-dependent manner. The calcium-dependent interaction of calmodulin and dystrophin does not directly regulate binding of actin to dystrophin, but may regulate dystrophin interactions with other associated proteins.
INSERM U 300, Faculté de Pharmacie, Montpellier, France.
A
A
Bonet-Kerrache
E
E
Fabbrizio
D
D
Mornet
D000199:Actins / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism*; D016189:Dystrophin / Q000737:chemistry / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D000199:Actins; D002147:Calmodulin; D016189:Dystrophin; D011994:Recombinant Proteins; D002118:Calcium