Synthesis of a novel bis(2,4'-bithiazole) derivative as a Co(II)-activated DNA cleaving agent.
A novel DNA cleaving agent, N,N'-bis(2-[4-(3-aminopropylcarbamoyl)-2,4'-bithiazol-2'-yl] ethyl) ethylenediamine (1), was synthesized. At 25 microM concentration, a significant cleaving activity of 1 for plasmid DNA was observed only in the presence of Co(II). Scavengers of active oxygen species could not inhibit the DNA cleavage by 1.
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
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Sasaki
D003035:Cobalt / Q000737:chemistry*; D004278:DNA, Superhelical / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D010957:Plasmids; D017382:Reactive Oxygen Species; D013844:Thiazoles / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism
D004278:DNA, Superhelical; D017382:Reactive Oxygen Species; D013844:Thiazoles; C090255:N,N'-bis(2-(4-(3-aminopropylcarbamoyl)-2,4'-bithiazol-2'-yl)ethyl)ethylenediamine; D003035:Cobalt