Hyperthermia inhibits proliferation and stimulates the expression of differentiation markers in cultured thyroid carcinoma cells.
In the last two decades hyperthermia has increasingly been used as adjuvant therapy for the treatment of malignant tumours. The effects of heat were therefore analysed on cultured thyroid epithelial cells from patients with thyroid cancer and from non-malignant control thyroids. Purified thyroid cells were subjected to heat treatment (42.5 degrees C; 90 min). After 24 h [3H]thymidine incorporation was assessed and the expression of heat shock protein 72 (hsp72), thyroglobulin, CD54 (ICAM-I) and MHC class-Il were analysed by immunofluorescence staining. Additionally mRNA analysis was performed by Northern blotting. Whereas hyperthermia inhibited the proliferation of thyroid cells, it significantly increased the expression of hsp72, thyroglobulin, CD54 and HLA-DR (P < 0.05). Our results suggest that hyperthermia may suppress growth while supporting differentiation and immune recognition in thyroid cancer. It may therefore be beneficial as a treatment for patients with thyroid carcinoma.
Institute for Biomedical Aging Research, Austrian Academy of Sciences, Innsbruck.
K
K
Trieb
A
A
Sztankay
A
A
Amberger
H
H
Lechner
B
B
Grubeck-Loebenstein
D018263:Adenocarcinoma, Follicular / Q000473:pathology* / Q000628:therapy; D000328:Adult; D000368:Aged; D015152:Blotting, Northern; D015153:Blotting, Western; D002277:Carcinoma / Q000473:pathology* / Q000628:therapy; D002291:Carcinoma, Papillary / Q000473:pathology* / Q000628:therapy; D002455:Cell Division; D002461:Cell Line, Transformed; D005260:Female; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D006684:HLA-DR Antigens / Q000096:biosynthesis; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D006358:Hot Temperature; D006801:Humans; D006979:Hyperthermia, Induced; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D013954:Thyroglobulin / Q000096:biosynthesis; D013964:Thyroid Neoplasms / Q000473:pathology* / Q000628:therapy; D014407:Tumor Cells, Cultured
D006684:HLA-DR Antigens; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; D013954:Thyroglobulin