Molecular cloning and sequencing of the capsid and the nuclear inclusion protein genes of a North American PVYN isolate.
The sequence of the 3'-terminal 3258 nucleotides of a tobacco veinal necrosis strain of a potato virus Y (PVYN) isolate from North America was determined. The sequence revealed an open reading frame of 2931 nucleotides, of which the start codon was not identified. The nontranslated region contains 327 nucleotides upstream of a poly(A) tract. The open reading frame encodes a large polyprotein containing 976 amino acids. The data indicate that the coat protein (CP) and the nuclear inclusion protein (NIb) are derived from the large polypeptide by proteolytic cleavage similar to many other potyviruses. The putative cleavage sites of NIa/NIb and NIb/CP correspond to Q/A and Q/G sequences, respectively, as in other PVYN isolates. The CP and NIb contain 267 and 519 amino acid residues, respectively. Higher sequence homology of CP was observed with other PVYN isolates than with the PVYO isolates.
Dhar
A K
AK
Agriculture and Agri-Food Canada, Fredericton, NB.
Singh
R P
RP
Boucher
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary; D012321:DNA-Directed RNA Polymerases; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D009509:New Brunswick; D010947:Plants, Toxic; D017800:Potyvirus / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000821:virology; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
D036022:Capsid Proteins; D018076:DNA, Complementary; D011498:Protein Precursors; D014764:Viral Proteins; D015678:Viral Structural Proteins; C090960:coat protein, Potato virus Y; C061396:nuclear inclusion protein b, mosaic viruses; D012321:DNA-Directed RNA Polymerases