Electrostatic cell-to-cell adhesion by a non-proteolytic component in a protease preparation.
A crude protease preparation from papaya (Protease Type III, Sigma) brought about reversible adhesion between adjacent cells of a variety of species including those of lymphoid, epithelial and fibroblastic origin at low ionic strength (below 100 mM). Sensitivities of the adhesion activity to ambient ionic strength, Ca2+, Mg2+ and pH indicated that the cell-cell contact is based on the electrostatic interaction. By ion-exchanger chromatography and gel-filtration chromatography it is suggested that the adhesion molecules in the protease preparation are dimers of cationic protein of 28 kDa distinct from the proteolytic component.
Yamamoto
T
T
Department of Physiology, Faculty of Medicine, Kyoto University, Japan.
Ohno-Shosaku
T
T
Furukawa-Uenoyama
E
E
Nagata
K
K
Okada
Y
Y
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004560:Electricity; D010450:Endopeptidases / Q000302:isolation & purification / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005638:Fruit / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials; D051379:Mice; D014411:Neoplastic Stem Cells / Q000187:drug effects; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D013045:Species Specificity
D010940:Plant Proteins; D010450:Endopeptidases; D008274:Magnesium; D002118:Calcium