Serum antioxidant status in streptozotocin-induced diabetic rat.
To determine whether decrease in serum antioxidants contributes to the increased oxidative stress, we measured the antioxidant activity (AOA), total peroxyl radical-trapping antioxidant parameter (TRAP), and their component individual antioxidants in the serum of diabetic rats. AOA was assayed as the ability of serum to inhibit lipid autoxidation in brain homogenates. TRAP was assayed as the ability to delay lipid peroxidation induced by an azo initiator. Antioxidants measured were ceruloplasmin, unsaturated iron binding capacity (UIBC) and albumin as components of AOA; and uric acid, protein sulfhydryl and alpha-tocopherol as components of TRAP. AOA was decreased markedly in the diabetics due to the decrease in ceruloplasmin, UIBC and albumin. On the other hand, the directly measured TRAP in the diabetics was unaltered. Uric acid and alpha-tocopherol were increased in the diabetics. However, decrease in unidentified scavengers offset the increase brought about by those antioxidants These results suggest that the decreased metal binding capacity contributes to the increased oxidative stress in the diabetic rats.
Department of Pediatrics, Yamanashi Medical College, Japan.
K
K
Asayama
T
T
Nakane
N
N
Uchida
H
H
Hayashibe
K
K
Dobashi
S
S
Nakazawa
D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D001786:Blood Glucose / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D019270:Fructosamine; D006595:Hexosamines / Q000097:blood; D007501:Iron / Q000097:blood; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D010545:Peroxides / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000097:blood; D014527:Uric Acid / Q000097:blood; D014810:Vitamin E / Q000097:blood
D000975:Antioxidants; D001786:Blood Glucose; D006595:Hexosamines; D010545:Peroxides; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D014810:Vitamin E; D014527:Uric Acid; C049375:perhydroxyl radical; D019270:Fructosamine; D007501:Iron; D002570:Ceruloplasmin