[Radiometric determination of Mycobacterium avium-intracellulare and Mycobacterium xenopi sensitivity to antitubercular agents].
Using the macrodilution radiometric technique in a BACTEC 460 apparatus, the authors assessed in 25 strains of the M. avium complex and 20 strains of M. xenopi the MIC of the following chemotherapeutic agents: Ciprofloxacine, clofazimine, rifampin, cycloserine, kanamycin, etionamide, ethambutol and amikacin. In all instances wild strains isolated in the Czech Republic in 1991-1992 were involved. In strains of the M. avium complex in the majority of drugs a major predominance of slightly or completely resistant strains was found. However, in addition to resistant strains there were also sensitive strains and strains with a reduced sensitivity in the group. The highest ratio of sensitive strains was recorded in clofazimine and amikacin. Strains of M. xenopi had, as compared with avian mycobacteria, lower MIC values with the exception of cycloserine and ethambutol in all the remaining chemotherapeutic agents. The applied radiometric technique was evaluated as a suitable method for assessment of the sensitivity of mycobacteria to chemotherapeutic agents, in particular because it provides results within a short time, i.e. within 8 days after inoculation and because of the quantitative evaluation of MIC values. It was therefore recommended for centralized assessment of sensitivity in the Czech Republic.
Státní zdravotní ústav, Praha.
M
M
Kubín
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P
Lindholm-Levy
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LB
Heifets
D000995:Antitubercular Agents / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015269:Mycobacterium avium Complex / Q000187:drug effects*; D009170:Nontuberculous Mycobacteria / Q000187:drug effects*; D011874:Radiometry
D000995:Antitubercular Agents
Radiometrické urcování citlivosti Mycobacterium avium-intracellulare a Mycobacterium xenopi na antituberkulotika.