Exercise training does not alter cytochrome P-450 content and microsomal metabolism.
The purpose of this investigation was to determine whether increased endurance exercise capacity alters total hepatic cytochrome P-450 content and cytochrome P-450 (CYP1A and CYP2B) mediated hepatic microsomal mixed-function oxidase drug metabolism. Twenty adult male Sprague-Dawley rats were randomly assigned to either a control (C) or an endurance trained group (ET). ET rats were progressively trained 5 d.wk-1 for 11 wk. Both C and ET rats were administered in random order single posttraining doses of probe drugs theophylline (probe for CYP1A) and antipyrine (probe for CYP2B). Soleus muscle citrate synthase activity of ET rats was significantly greater (P < 0.01) than for C rats (mean +/- SD; C, 26.4 +/- 1.3 mumol.g-1.min-1; ET, 46.1 +/- 2.7). In contrast, total liver cytochrome P-450 content was not significantly different (P > 0.01) among C and ET rats (mean +/- SD; C, 0.554 +/- 0.055 nmol.mg-1 liver protein; ET, 0.604 +/- 0.080). Likewise, the posttraining C and ET single-sample plasma clearances of theophylline (mean +/- SD; C, 1.89 +/- 0.360 1.h-1.kg-1 total liver weight; ET, 2.08 +/- 0.49) and antipyrine (mean +/- SD; C, 6.44 +/- 1.56 1.h-1.kg-1 total liver weight; ET, 6.51 +/- 1.02) were not significantly different (P > 0.01). Therefore, it was concluded that strenuous endurance training of 11 wk duration did not alter total hepatic cytochrome P-450 content or CYP1A or CYP2B activity.
Michaud
T J
TJ
Department of Health Promotion and Human Performance, University of Toledo, OH 43606.
Bachmann
K A
KA
Andres
F F
FF
Flynn
M G
MG
Sherman
G P
GP
Rodriguez-Zayas
J
J
D000818:Animals; D000983:Antipyrine / Q000097:blood / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D008657:Metabolic Clearance Rate; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000201:enzymology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D010807:Physical Endurance; D051381:Rats; D017207:Rats, Sprague-Dawley; D012420:Running / Q000502:physiology; D013806:Theophylline / Q000097:blood / Q000378:metabolism
D003577:Cytochrome P-450 Enzyme System; D013806:Theophylline; D006899:Mixed Function Oxygenases; D002950:Citrate (si)-Synthase; D000983:Antipyrine