Overexpression of c-myc precedes amplification of the gene encoding dihydrofolate reductase.
An experimentally inducible model system was generated in which Chinese hamster ovary cells (CHO-9) were stably transfected with an inducible c-myc cDNA. The induction of c-myc in these transfectants is followed by the enhanced binding of c-Myc/Max-containing protein complexes to 5'flanking E-box sequences of the gene encoding dihydrofolate reductase (DHFR). Moreover, DHFR is transiently amplified. The inappropriate overproduction of the oncoprotein, therefore, seems to plays a role in induced DHFR amplification.
Basel Institute for Immunology, Switzerland.
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D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D018076:DNA, Complementary; D005784:Gene Amplification; D016259:Genes, myc; D017404:In Situ Hybridization, Fluorescence; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014162:Transfection
D018076:DNA, Complementary; D013762:Tetrahydrofolate Dehydrogenase