Capillary electrophoretic examination of underivatized O-linked and N-linked oligosaccharide mixtures and immunoglobulin G antibody-released oligosaccharide libraries.
A procedure for the analysis of mixtures of underivatized, cleaved O-linked and N-linked oligosaccharides by capillary electrophoresis has been developed. The species of interest are separated by two borate-based buffer systems with an uncoated capillary. The procedure is applied to hydrazinolysis-released oligosaccharides from mouse, rat, sheep and human IgG protein samples which are examined within 6 minutes. Selectivity with respect to sample variation is demonstrated by analysis of thermally stressed samples.
Pharmaceutical Research Institute, Bristol-Myers Squibb Company, Syracuse, NY 13221-4755.
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Hughes
D000818:Animals; D002240:Carbohydrate Sequence; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000276:immunology / Q000302:isolation & purification*; D051381:Rats; D012756:Sheep
D007074:Immunoglobulin G; D009844:Oligosaccharides