Expression of an Onchocerca volvulus Ov33 homolog in Dirofilaria immitis: potential in immunodiagnosis of heartworm infection.
In this study, the expression of an Onchocerca volvulus Ov33 homolog was demonstrated in Dirofilaria immitis. Rabbit antiserum raised against a recombinant fusion protein of O. volvulus, MBP/OvD 5B (Ov33), was found to react with a 31-33 kDa glycoprotein (DiT33) of adult worms of D. immitis. An antibody response to MBP/OvD 5B was observed in dogs, as early as 11 weeks post infection with infective larvae of D. immitis, and in dogs with occult infection. Cats both experimentally and naturally infected with D. immitis also reacted strongly with the recombinant antigen. In contrast, sera from dogs receiving chemically-abbreviated infection or from animals harbouring a variety of other helminths failed to react. These data suggest that antibody responses generated by DiT33 may have potential in immunodiagnosis of heartworm infection in cats and dogs.
Santiago Mejia
J
J
New England Biolabs, Beverly, MA 01915.
Nkenfou
C
C
Southworth
M W
MW
Perler
F B
FB
Carlow
C K
CK
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D002371:Cat Diseases / Q000175:diagnosis / Q000276:immunology; D002415:Cats; D003429:Cross Reactions; D004183:Dirofilaria immitis / Q000235:genetics / Q000276:immunology*; D004184:Dirofilariasis / Q000175:diagnosis* / Q000276:immunology; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D015801:Helminth Proteins / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D017181:Onchocerca volvulus / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins