Ethylene production by strains of the plant-pathogenic bacterium Pseudomonas syringae depends upon the presence of indigenous plasmids carrying homologous genes for the ethylene-forming enzyme.
The molecular characteristics of the ethylene-forming enzymes of strains of Pseudomonas syringae were tested. The ethylene-producing activities of the nine strains as measured in vivo and in vitro were similar, except for that of P. syringae pv. mori M5. A polyclonal antibody and a DNA probe for the ethylene-forming enzyme from P. syringae pv. phaseolicola PK2 were prepared to investigate homologies among the proteins and genes for the ethylene-forming enzymes. With the exception of P. syringae pv. mori M5, eight strains tested expressed the same antigen as the ethylene-forming enzyme from P. syringae pv. phaseolicola PK2 and were homologous to DNA sequences on indigenous plasmids. Molecular masses of antigenic proteins from all ethylene-producing strains were 40 kDa. The N-terminal amino acid sequence of the purified ethylene-forming enzyme from P. syringae pv. glycinea KN130 was identical to that of the enzyme from P. syringae pv. phaseolicola PK2. These results show that the ethylene-forming enzymes encoded by the indigenous plasmid(s) in the pathogenic bacteria examined were similar.
Nagahama
K
K
Department of Applied Microbial Technology, Kumamoto Institute of Technology, Japan.
Yoshino
K
K
Matsuoka
M
M
Sato
M
M
Tanase
S
S
Ogawa
T
T
Fukuda
H
H
D000595:Amino Acid Sequence; D005030:Ethylenes / Q000096:biosynthesis*; D005798:Genes, Bacterial; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000382:microbiology; D010957:Plasmids / Q000235:genetics*; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity; D013045:Species Specificity
D005030:Ethylenes; C036216:ethylene; D008190:Lyases; C072390:ethylene-forming enzyme, Pseudomonas