Hybrid proteins between Pseudomonas exotoxin A and poliovirus protease 2Apro.
Two hybrid proteins between Pseudomonas aeruginosa exotoxin A (PE) and poliovirus protease 2Apro have been generated. One hybrid protein contains the poliovirus 2Apro sequence replacing the region of PE corresponding to amino acids 413-607. The other hybrid contains in addition the transforming growth factor sequence. The two hybrid proteins were efficiently synthesized in E. coli cells using the inducible pET vectors. Both hybrid toxins cleaved p220 (eIF-4 gamma) when the recombinant plasmids were transfected in COS cells infected with recombinant vaccinia virus bearing the T7 RNA polymerase gene.
Centro de Biología Molecular, UAM-CSIC, Universidad Autónoma de Madrid, Spain.
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Novoa
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Feduchi
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Carrasco
D036002:ADP Ribose Transferases; D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D005098:Exotoxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010448:Peptide Initiation Factors / Q000378:metabolism; D011550:Pseudomonas aeruginosa; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014162:Transfection; D014764:Viral Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D003546:Cysteine Endopeptidases; D005098:Exotoxins; D010448:Peptide Initiation Factors; D011994:Recombinant Proteins; D014764:Viral Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C077382:eIF-4gamma; C057400:picornain 2A, Picornavirus