Specific thiol compounds complement deficiency in c-type cytochrome biogenesis in Escherichia coli carrying a mutation in a membrane-bound disulphide isomerase-like protein.
Escherichia coli JCB606 carries a mutation in the dipZ gene, known to code for a disulphide isomerase-like protein, with the consequence that holo forms of neither exogenous nor endogenous c-type cytochromes are synthesised. This failure has been overcome by adding compounds containing thiol groups to the growth medium. Only L-cysteine and 2-mercaptoethane sulphonic acid were effective, suggesting a (stereo)specific binding site that could be occupied by these compounds in the absence of the catalytic domain of DipZ.
Department of Biochemistry, University of Oxford, UK.
Y
Y
Sambongi
S J
SJ
Ferguson
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002458:Cell Fractionation; D003470:Culture Media; D003574:Cytochrome c Group / Q000096:biosynthesis* / Q000235:genetics; D004220:Disulfides / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D007535:Isomerases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology*; D019704:Protein Disulfide-Isomerases; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D003470:Culture Media; D003574:Cytochrome c Group; D004220:Disulfides; D011993:Recombinant Fusion Proteins; D013438:Sulfhydryl Compounds; D000469:Alkaline Phosphatase; D007535:Isomerases; D019704:Protein Disulfide-Isomerases