Bacteriophages as tools for vaccine development.
The construction of live recombinant bacterial vaccines requires a reasonably sophisticated genetic system for the introduction, stabilization and expression of foreign antigen genes. Bacteriophages offer a rich collection of tools that can be used for vaccine construction, including site-specific integration-proficient vectors, non-antibiotic selectable markers and signals for efficient transcription and translation of foreign genes. We describe the characterization of a temperate phage of the mycobacteria, mycobacteriophage L5, and application of these phage studies for the construction of recombinant BCG vaccines.
Department of Biological Sciences, University of Pittsburgh, PA.
G F
GF
Hatfull
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L
Barsom
L
L
Chang
M
M
Donnelly-Wu
M H
MH
Lee
M
M
Levin
C
C
Nesbit
G J
GJ
Sarkis
D001500:BCG Vaccine; D001435:Bacteriophages / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D016679:Genome, Viral; D008242:Lysogeny; D009163:Mycobacterium bovis / Q000821:virology; D015163:Superinfection; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic
D001500:BCG Vaccine; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic