Chemotactic activity of substances derived from antibody-loaded tumor cells on granulocytes.
Chemotactic activity of granulocytes attracted by tumor cells loaded either with anti-ganglioside monoclonal antibodies (mAb) or with antibody-glucose oxidase conjugates (mAb-GO) was investigated. The melanoma cell line SK-Mel-28 which expresses the ganglioside GD3 at high density as well as the neuroectodermal cell line SK-N-LO which expresses GD2 were used for the experiments. In the presence of 50% human AB-serum, antibody-loaded tumor cells induced chemotactic activity on granulocytes, probably due to the generation of C3a/C5a which could be detected in serum incubated with anti-GD3 loaded SK-Mel-28 cells. Both compounds could also be detected in vivo in the plasma of patients suffering from neuroblastoma during therapy with anti-GD2 antibodies. In another set of experiments mAb-GO conjugates generating high amounts of H2O2 in the presence of glucose were bound to these tumor cells. A significant lipid peroxidation could be observed in the simultaneous presence of iron and ascorbate. The lipid peroxidation products were measured as thiobarbituric acid-reactive substances (TBARS) and were also shown to induce chemotactic effects on granulocytes.
Schaber
B
B
Department of Dermatology, Children's Hospital, Tuebingen, Germany.
Bruchelt
G
G
Meyle
J
J
Jeschke
B
B
Handgretinger
R
R
Niethammer
D
D
Mayer
P
P
Rassner
G
G
Fierlbeck
G
G
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D002630:Chemotactic Factors / Q000096:biosynthesis*; D002633:Chemotaxis; D015926:Complement C3a / Q000032:analysis; D015936:Complement C5a / Q000032:analysis; D005732:Gangliosides / Q000276:immunology; D005949:Glucose Oxidase / Q000276:immunology; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation; D008545:Melanoma / Q000276:immunology / Q000473:pathology; D017599:Neuroectodermal Tumors / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D002630:Chemotactic Factors; D005732:Gangliosides; C026226:ganglioside, GD3; C019403:ganglioside, GD2; D015926:Complement C3a; D015936:Complement C5a; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase