Effects of iron-induced lipid peroxidation and of acidosis on choline uptake by synaptosomes.
The effects of iron-induced lipid peroxidation and of lactic acidosis on [3H]choline uptake were investigated on crude synaptosomes prepared from rat cerebral cortices. Fe(2+)-induced lipid peroxidation as evidenced from the production of thiobarbituric acid reactives substances (TBARS) was correlated with a decrease in high-affinity choline uptake (HACU). Trolox C, a free radical scavenger, prevented both Fe(2+)-induced TBARS production and decrease in HACU. Lactic acidosis (pH 6.0 for 30 or 60 min) increased the TBARS production with concomitant decrease in HACU (-48%, -78%, respectively). The acidosis dependent decrease was not reversible following pH 7.4 readjustment after 60 min acidosis. It was not prevented by trolox C, although trolox C inhibited the acidosis-induced production of TBARS. The results suggest that the contribution of acidosis to peroxidative damages is probably of less importance in comparison to other cytotoxic mechanisms.
Cancela
J M
JM
Laboratoire de Pharmacodynamie, Faculté de Pharmacie, Dijon, France.
Bralet
J
J
Beley
A
A
D000140:Acidosis, Lactic / Q000378:metabolism*; D000818:Animals; D002794:Choline / Q000378:metabolism*; D005296:Ferrous Compounds / Q000494:pharmacology*; D005609:Free Radicals; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism
D005296:Ferrous Compounds; D005609:Free Radicals; D002794:Choline