Evidence for naturally occurring recombination in the gene encoding the major outer membrane protein of lymphogranuloma venereum isolates of Chlamydia trachomatis.
The nucleotide sequence of the major outer membrane protein gene (omp1) was determined for three geographically distinct lymphogranuloma venereum isolates which were serologically untypeable. The three omp1 sequences were hybrids of serovars L1 and L2, containing a putative DNA recombination site in variable segment 2. Efforts to manipulate the chlamydial genome in vitro by recombination should be intensified.
Southampton University Medical School, Southampton General Hospital, United Kingdom.
L J
LJ
Hayes
P
P
Yearsley
J D
JD
Treharne
R A
RA
Ballard
G H
GH
Fehler
M E
ME
Ward
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D002690:Chlamydia Infections / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000145:classification / Q000235:genetics*; D015818:Eye Infections, Bacterial / Q000382:microbiology; D005260:Female; D006801:Humans; D008219:Lymphogranuloma Venereum / Q000453:epidemiology / Q000382:microbiology*; D008969:Molecular Sequence Data; D018272:Porins; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D013019:South Africa / Q000453:epidemiology
D001425:Bacterial Outer Membrane Proteins; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis