Separation of fifteen non-steroidal anti-inflammatory drugs using micellar electrokinetic capillary chromatography.
Micellar electrokinetic capillary chromatography with sodium dodecylsulfate as anionic surfactant was used to separate simultaneously fifteen non-steroidal anti-inflammatory drugs. UV detection was performed at 254 nm. The electroosmotic flow was carefully adjusted for optimal separation. Resolution of the drugs was obtained using a buffer containing 40 mmol l-1 NaH2PO4, 0.104 mol l-1 sodium dodecylsulfate and 3% (v/v) methanol, adjusted to pH 8 with sodium hydroxide. Lack of interference was checked with a number of drugs and metabolites. Between-day coefficients of variation ranged between 2 to 10%.
Department of Clinical Pharmacology, Jean Minjoz University Hospital, Besançon, France.
C W
CW
Maboundou
G
G
Paintaud
M
M
Bérard
P R
PR
Bechtel
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification*; D002197:Capillary Action; D002845:Chromatography / Q000379:methods* / Q000706:statistics & numerical data; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D008823:Micelles; D010710:Phosphates; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008823:Micelles; D010710:Phosphates; D012967:Sodium Dodecyl Sulfate; C018279:sodium phosphate; D000432:Methanol