Assignment of the human a-tropomyosin gene TPM3 to 1q22-->q23 by fluorescence in situ hybridisation.
The human tropomyosin 3 (TPM3) gene was previously localized to chromosome 1. The non-muscle isoform of the TPM3 gene product becomes fused to a gene product from the tyrosine kinase receptor gene (NTRK1), previously localized to 1q23-->q24, to generate an active oncogene. Two sequence tagged sites spanning three exons and two introns in the carboxy coding region of the gene were used to localize TPM3 to 1q22-->q23 by fluorescence in situ hybridization. This localization now places the NTRK1 and TPM3 genes in close proximity, so that a gene fusion rearrangement would not be cytologically detected. The 1q22-->q23 localization of TPM3 is within the NEM1 locus associated with autosomal dominant nemaline myopathy, making TPM3 a candidate for this disorder.
Australian Neuromuscular Research Institute, QEII Medical Centre, Nedlands.
S D
SD
Wilton
H
H
Eyre
P A
PA
Akkari
H C
HC
Watkins
C
C
MacRae
N G
NG
Laing
D C
DC
Callen
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D017931:DNA Primers; D005091:Exons; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D020917:Receptor, trkA; D017475:Receptors, Nerve Growth Factor / Q000235:genetics; D012016:Reference Values; D016324:Sequence Tagged Sites; D014335:Tropomyosin / Q000235:genetics*
D017931:DNA Primers; D011518:Proto-Oncogene Proteins; D017475:Receptors, Nerve Growth Factor; D014335:Tropomyosin; D020794:Receptor Protein-Tyrosine Kinases; D020917:Receptor, trkA