Photochemiluminescent detection of antiradical activity; III: A simple assay of ascorbate in blood plasma.
A simple procedure to determine the ascorbate in blood plasma was elaborated based on a temporary inhibition of the photo-induced, O(-.)2-mediated, chemiluminescence emanating from oxidation of luminol. To remove the interferences, mainly from urate, a simple enzyme-free one-step method for plasma sample preparation by means of a gel filtration was combined with the photochemiluminescent investigation of the eluate. The results of the photochemiluminescent assay of ascorbate coincide well (r = 0.994) with a photometric method with ascorbate oxidase. The mean value of ascorbate in human blood plasma, obtained from 37 healthy volunteers, was 59.6 +/- 25.8 mumol/l. Patients with atherosclerosis having a myocardial infarction in their anamnesis displayed significantly lower values: 27.2 +/- 12.8 mumol/l (n = 32), P < 0.001.
Lewin
G
G
Research Laboratory for Antioxidant Therapy, Medical Faculty (Charité), Humboldt University of Berlin, Germany.
Popov
I
I
D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000097:blood*; D001681:Biological Assay; D005609:Free Radicals; D006801:Humans; D008163:Luminescent Measurements; D010777:Photochemistry; D012995:Solubility; D014867:Water / Q000737:chemistry
D000975:Antioxidants; D005609:Free Radicals; D014867:Water; D001205:Ascorbic Acid