Comparison of two digestion methods for the determination of selenium in biological samples.
Two digestion methods for the determination of selenium in biological samples have been compared: a classic digestion in a heating block by means of wet acid-ashing (HNO3-HClO4) and a microwave digestion in an open-vessel system by heating under reflux with HNO3-H2O2. The two methods were used to determine Se in plasma samples and different reference materials. There was no difference between the mean results, and the correlation coefficient for wet acid-ashing versus microwave digestion was 0.94. The two methods yielded accurate results for the certified materials analysed.
Ducros
V
V
Laboratoire de Biochimie C, Hôpital Michallon, Grenoble, France.
Ruffieux
D
D
Belin
N
N
Favier
A
A
D006801:Humans; D008872:Microwaves; D012044:Regression Analysis; D012643:Selenium / Q000032:analysis*
D012643:Selenium