Cell type-dependence for Vpu function.
Human immunodeficiency virus type 1 Vpu has been shown to facilitate virus release from HeLa cells. We demonstrated that Vpu expression is not required for efficient virus release from Cos 1 and CV-1 cells. A yeast GAL4 transcriptional activation system was used to screen for cellular proteins that may interact with Vpu. One such protein was identified which we provisionally designate "Vpu interactive protein" or VIP.
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
R J
RJ
Geraghty
K J
KJ
Talbot
M
M
Callahan
W
W
Harper
A T
AT
Panganiban
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000096:biosynthesis / Q000378:metabolism; D005814:Genes, Viral; D016338:Genes, vpu; D005816:Genetic Complementation Test; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D054298:Human Immunodeficiency Virus Proteins; D006801:Humans; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins / Q000378:metabolism*; D000086082:Viroporin Proteins
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D054298:Human Immunodeficiency Virus Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D054334:Viral Regulatory and Accessory Proteins; C052130:GAL4 protein, S cerevisiae; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins