Primary cultures of rhesus placental syncytiotrophoblasts are permissive for SIV infection.
Primary cultures of rhesus syncytiotrophoblasts incubated with SIVdeltaB670, SIVmac251, or SIVmac239 produced readily detectable virus in the supernatant for up to three weeks after infection. At four weeks, cells generally failed to release virus but placental cell lysates and placental cells cocultured for 24 hours with uninfected CEM x 174 cells were able to transmit infection. The presence of virus was confirmed by electron microscopy and PCR amplification of viral sequences from trophoblast genomic DNA. SIV p27 antigen was localized by immunostaining primarily in syncytiotrophoblasts.
Wisconsin Regional Primate Research Center, University of Wisconsin, Madison 53715-1299.
T G
TG
Golos
L A
LA
Krugner-Higby
C S
CS
Williams
J M
JM
Fisher
K J
KJ
Johnson
M
M
Durning
K T
KT
Schultz
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D015683:Gene Products, gag / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D010920:Placenta / Q000166:cytology* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015302:Simian Immunodeficiency Virus / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D014327:Trophoblasts / Q000166:cytology* / Q000382:microbiology / Q000648:ultrastructure; D014779:Virus Replication
D017931:DNA Primers; D004279:DNA, Viral; C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag