Aromatic amino acid metabolism of neonatal piglets receiving TPN: effect of tyrosine precursors.
Low tyrosine solubility in total parenteral nutrition (TPN) solutions complicates meeting the aromatic amino acid needs of infants. This study compared the effectiveness of two tyrosine precursors to supply the aromatic amino acid needs of TPN-fed neonatal piglets with a control group in which total aromatic acid needs were met by the addition of phenylalanine (Phe). Eighteen 3-day-old male Yorkshire piglets (6/group) received TPN for 8 days by central line. The solution was supplemented with Phe or one of the following two tyrosine precursors: N-acetyltyrosine (N-AcTyr) or glycyltyrosine (GlyTyr). Aromatic amino acid metabolism, growth, and nitrogen utilization were measured. Average amino acid and energy intakes were 14.6 g.kg-1.day-1 and 1,050 kJ.kg-1.day-1. Nitrogen balance and utilization were significantly higher (P < 0.05) in piglets in the control Phe group and on the GlyTyr regimen. The high urinary excretion of N-AcTyr (65%) confirms its low bioavailability. Flux and oxidation were significantly higher (P < 0.05) in the Phe group. High plasma Phe levels and excretion of Phe catabolites, as well as the high plasma tyrosine in the GlyTyr group, indicate that current strategies employed to meet the aromatic amino acid needs of neonates on TPN need further refinement.
Wykes
L J
LJ
Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
House
J D
JD
Ball
R O
RO
Pencharz
P B
PB
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D001823:Body Composition; D004151:Dipeptides / Q000494:pharmacology*; D008297:Male; D009584:Nitrogen / Q000378:metabolism; D010289:Parenteral Nutrition, Total; D010649:Phenylalanine / Q000494:pharmacology*; D013552:Swine; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000494:pharmacology
D000596:Amino Acids; D004151:Dipeptides; D014443:Tyrosine; D010649:Phenylalanine; C022013:glycyltyrosine; C025787:N-acetyltyrosine; D009584:Nitrogen