Type 1 prophospholipase A2 propeptide in acute lung injury.
Neutrophil sequestration and activation in the pulmonary vasculature and interstitium are important in acute lung injury. Phospholipase A2 plays an important part in the production of potent inflammatory mediators in this syndrome. We used our ELISA for type 1 prophospholipase A2 activation peptides, which have the aminoacid sequence Asp-Ser-Gly-Ile-Ser-Pro-Arg (DSGISPR), to show that DSGISPR concentrations in plasma and urine are a sensitive and specific marker of acute lung injury in patients admitted to intensive care. The detection of DSGISPR in the plasma of 11 of 50 unselected patients had a sensitivity of 100% and a specificity of 93% for the presence or future development of acute lung injury.
Department of Surgery, St George's Hospital Medical School, London, UK.
D
D
Rae
J
J
Porter
N
N
Beechey-Newman
N
N
Sumar
D
D
Bennett
J
J
Hermon-Taylor
D000595:Amino Acid Sequence; D015415:Biomarkers; D004792:Enzyme Precursors; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism; D008171:Lung Diseases / Q000201:enzymology* / Q000276:immunology; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology; D010741:Phospholipases A / Q000097:blood* / Q000378:metabolism / Q000652:urine; D054467:Phospholipases A2; D011498:Protein Precursors / Q000097:blood* / Q000378:metabolism / Q000652:urine; D012680:Sensitivity and Specificity
D015415:Biomarkers; D004792:Enzyme Precursors; D018836:Inflammation Mediators; D011498:Protein Precursors; D010741:Phospholipases A; D054467:Phospholipases A2; C085333:prophospholipase A2