Different pattern of T cell receptor delta gene rearrangement in tumour-infiltrating lymphocytes and peripheral blood in patients with solid tumours.
Tumour-infiltrating lymphocytes (TIL) and peripheral blood lymphocytes (PBL) from four patients with renal-cell carcinoma (three paired with blood), two colon carcinomas (both paired with blood) and two melanomas (blood was not available) were analysed for the T cell receptor (TCR) delta gene repertoire. Polymerase chain reaction analysis, employing a panel of specific primers for TCR delta gene segments, showed different gene rearrangement patterns in TIL and PBL in all patients. Simultaneous analysis of TIL and PBL revealed the presence of lymphoid cells in the tumour tissue that were not present in the periphery. These results demonstrate that, although tumour-infiltrating lymphocytes contain gamma/delta T cells within the range observed in peripheral blood, these cells differ from those in peripheral blood in their gene repertoire and this may account for selective accumulation or/and in situ amplification of gamma/delta lymphocytes at the tumour site, indicating a unique type of host reaction against tumour.
Institute of Human Genetics, Polish Academy of Sciences, Poznań.
D
D
Kowalczyk
W
W
Skorupski
M
M
Drews
J
J
Nowak
D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000097:blood / Q000235:genetics; D003110:Colonic Neoplasms / Q000097:blood / Q000235:genetics; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D006801:Humans; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007680:Kidney Neoplasms / Q000097:blood / Q000235:genetics; D008214:Lymphocytes / Q000502:physiology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000502:physiology*; D008545:Melanoma / Q000097:blood / Q000235:genetics / Q000556:secondary; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000097:blood* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D012680:Sensitivity and Specificity
D007144:Immunoglobulin J-Chains; D007135:Immunoglobulin Variable Region; D016692:Receptors, Antigen, T-Cell, gamma-delta