Glucose-6-phosphatase gene expression and activity are modulated in hemorrhagic shock: evidence for a new heat-sensitive activator.
Decreased hepatic fructose 2,6-bisphosphate levels were observed in the early phase of hemorrhagic shock. The lower sugar bisphosphatae level was a result of increased phosphoenolpyruvate levels and decreased glucose-6-phosphate and fructose-6-phosphate levels. The decreased glucose-6-phosphate levels correlated with increased activity of liver glucose-6-phosphatase and a concomitant 2.5-fold increase in glucose-6-phosphatase mRNA abundance. In addition, protein-free filtrate from hemorrhagic shock rats, but not from control rats, increased glucose-6-phosphatase activity. However, when control and hemorrhagic shock protein-free filtrates were heated, they both increased the glucose-6-phosphatase activity of the respective microsomes to the same extent. It is concluded that the early hyperglycemic phase of hemorrhagic shock is due to enhanced glucose-6-phosphatase gene expression and activity and the generation of a heat sensitive activator of the enzyme.
Department of Emergency Medicine, State University of New York at Stony Brook 11794.
S R
SR
Maitra
W
W
Pan
A J
AJ
Lange
M R
MR
el-Maghrabi
N N
NN
Abumrad
S J
SJ
Pilkis
D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D005943:Gluconeogenesis; D005952:Glucose-6-Phosphatase / Q000235:genetics* / Q000378:metabolism; D006019:Glycolysis; D006358:Hot Temperature; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000201:enzymology*
D012333:RNA, Messenger; D005952:Glucose-6-Phosphatase