Polymerase chain reaction in the diagnosis of chromosomal breakpoints.
Polymerase chain reaction-based methods for the detection of translocation-induced gene rearrangements are now widely used for diagnostics and patient monitoring. This article concentrates on two of the best studied chromosome translocations resulting in specific gene rearrangements and oncogene activation: the Philadelphia translocation of chronic myelogenous leukemia and acute leukemias, and the t(14;18) translocation of follicular lymphomas.
Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, Michigan.
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Crisan
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Chen
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Weil
D002869:Chromosome Aberrations; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D015321:Gene Rearrangement; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D008223:Lymphoma / Q000235:genetics*; D010677:Philadelphia Chromosome; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic
D016044:Fusion Proteins, bcr-abl