GABAA receptor beta 1-3 subunit gene expression in the hippocampus of kindled rats.
The effect of Schaffer collateral/commissural fiber kindling on the expression levels of GABAA receptor beta 1, beta 2 and beta 3 subunit mRNA in the pyramidal and granular neurons of the rat dorsal hippocampus was studied, using semi-quantitative in situ hybridization. In pyramidal neurons of CA1 and CA3, only small changes (10-15%) were found. In dentate granule neurons, the expression level of GABAA R-beta 3 mRNA was significantly, enhanced, bilaterally, in animals that were partial or fully kindled. At long-term, 4 weeks after the last convulsion no significant changes were found in pyramidal or granular neurons.
Graduate School for the Neurosciences, University of Amsterdam, Institute of Neurobiology, The Netherlands.
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W
Kamphuis
T C
TC
De Rijk
F H
FH
Lopes da Silva
D000818:Animals; D015870:Gene Expression / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D007696:Kindling, Neurologic / Q000502:physiology*; D008297:Male; D017966:Pyramidal Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D011963:Receptors, GABA-A