Vaccinia virus expression of the VP7 protein of South African bluetongue virus serotype 4 and its use as an antigen in a capture ELISA.
Recombinant vaccinia viruses expressing the VP7 core protein of South African bluetongue virus serotype 4 (SA-BTV4) were identified by polymerase chain reaction amplification. Expression of VP7 was verified by radio-immunoprecipitation and a F(ab')2-based ELISA. Antibodies to VP7 were detected in sera from sheep that had been infected with 20 different virulent BTV serotypes by using the vaccinia virus (VV) expressed VP7 as antigen in a capture ELISA. F(ab')2-immobilised VV-expressed SA-BTV4 VP7 cross-reacted with sera directed against all 9 African horsesickness virus serotypes and epizootic haemorrhagic disease virus serotype 2.
Biochemistry Division, Onderstepoort Veterinary Institute, Republic of South Africa.
M
M
Cloete
D H
DH
du Plessis
A A
AA
van Dijk
H
H
Huismans
G J
GJ
Viljoen
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001483:Base Sequence; D001820:Bluetongue virus / Q000235:genetics / Q000378:metabolism*; D002213:Capsid / Q000032:analysis* / Q000096:biosynthesis* / Q000235:genetics; D036022:Capsid Proteins; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015870:Gene Expression; D006168:Guinea Pigs / Q000276:immunology; D006736:Horses / Q000276:immunology; D007106:Immune Sera; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D011995:Recombination, Genetic; D013019:South Africa; D014616:Vaccinia virus / Q000378:metabolism*; D014709:Vero Cells
D000956:Antigens, Viral; D036022:Capsid Proteins; D017931:DNA Primers; D007106:Immune Sera; C050779:VP7 protein, Rotavirus