Titration of numbers of human-derived Mycobacterium leprae required to progressively oxidize 14C-palmitic acid and release 14CO2.
Mycobacterium leprae was isolated from skin-punch biopsies of 2 untreated lepromatous leprosy patients. The bacteria were enumerated, diluted 10-fold and cultured in Middlebrook 7H9 medium supplemented with albumin, dextrose, catalase and 14C-palmitic acid. The cultures were incubated at 33 degrees C in a modified Buddemeyer radiorespiratory detection vessel. Those cultures containing at least 10(7) mycobacteria demonstrated a progressive evolution of 14CO2.
Gillis W. Long Hansen's Disease Center, Carville, Louisiana.
E J
EJ
Shannon
D
D
Frommel
M
M
Guebre-Xabier
H S
HS
Haile-Mariam
D002245:Carbon Dioxide / Q000378:metabolism*; D015169:Colony Count, Microbial; D006801:Humans; D015440:Leprosy, Lepromatous / Q000382:microbiology*; D009166:Mycobacterium leprae / Q000378:metabolism*; D010084:Oxidation-Reduction; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D012867:Skin / Q000382:microbiology
D010169:Palmitic Acids; D002245:Carbon Dioxide; D019308:Palmitic Acid