Cyclin A and cyclin B dissociate from p34cdc2 with half-times of 4 and 15 h, respectively, regardless of the phase of the cell cycle.
The exchange rate of cyclin A and cyclin B between endogenous p34cdc2 and bacterially expressed GST-cdc2 was measured in concentrated Xenopus egg extracts. The half-lives of the cyclin A.p34cdc2 and the cyclin B.p34cdc2 complexes are estimated as 4 and 15 h, respectively. There is no significant difference in these affinities when they are measured in mitosis, interphase, or during transition between these two states. The tight association between cyclin and p34cdc2 in the cell cycle may be significant for mechanisms of cyclin destruction.
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, United Kingdom.
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Kobayashi
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Stewart
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Poon
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Hunt
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002453:Cell Cycle; D016213:Cyclins / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D006207:Half-Life; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D014981:Xenopus
D016213:Cyclins; D009838:Oligodeoxyribonucleotides; D005982:Glutathione Transferase; D016203:CDC2 Protein Kinase