The development of glial fibrillary acidic protein-positive cells and the appearance of laminin-like immunoreactivity in fetal olfactory bulb transplants.
Embryonic rat olfactory bulbs were transplanted into the site vacated by aspiration of an olfactory bulb from a neonatal rat. This paper presents our findings related to the development of glial fibrillary acidic protein (GFAP) positive (+ve) glial cells and the appearance of laminin-like immunoreactivity in these transplants. The GFAP+ve glial cells formed perivascular end-feet on the invading vasculature and formed a glia limitans along the pial surface of the transplant. This reconstituted glia limitans was continuous with that of the host brain, there being no glia limitans at the donor-host interface. Thus, the donor tissue was well-integrated with that of the host brain.
Department of Anatomy, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
R
R
Doucette
J
J
Kott
L
L
Westrum
D000818:Animals; D016380:Brain Tissue Transplantation / Q000502:physiology*; D002454:Cell Differentiation; D005260:Female; D016332:Fetal Tissue Transplantation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology* / Q000378:metabolism*; D007150:Immunohistochemistry; D007797:Laminin / Q000276:immunology / Q000378:metabolism*; D009457:Neuroglia / Q000502:physiology; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000637:transplantation*; D016612:Paraffin Embedding; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D005904:Glial Fibrillary Acidic Protein; D007797:Laminin