Characterization of the mouse gene encoding the neuronal intermediate filament protein alpha-internexin.
We have determined the complete nucleotide (nt) sequence of the coding region of the mouse gene encoding the neuronal intermediate filament protein, alpha-internexin (alpha INX). The mouse alpha INX gene (m alpha INX) contains three exons and two introns, and may be classified as a member of the type-IV intermediate filament multigene family. The nt sequence of the transcribed region of m alpha INX shows high homology to that of the rat gene. Comparison of the deduced amino-acid sequences between the mouse and rat gene products reveals that the head and rod domains are highly conserved. However, the tail domains show significant differences which may make it possible to raise specific antibodies that can distinguish the alpha INX of mouse from that of rat.
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, NY 10032.
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Liem
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005091:Exons; D007381:Intermediate Filament Proteins / Q000235:genetics*; D007438:Introns; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats / Q000235:genetics; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D002352:Carrier Proteins; D007381:Intermediate Filament Proteins; D012333:RNA, Messenger; C046643:alpha-internexin