Spectrofluorometric method for NO determination.
A spectroscopic method for the quantitative determination of NO in aqueous solution is presented. The method is based on the uv absorbance of nitrosothiols, coupled with the inner filter effect. A threshold of 5.10(-7) M with a wide dynamic range of 5.10(-7)-10(-4) M are obtained. This method enables measurements in brain homogenates. Ultimately, since this method is immune to electrical perturbances, it will be applied at the tip of an optical fiber for in situ monitoring of NO or glutathione in brain and blood vessels.
Israel Institute for Biological Research.
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Gabor
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Allon
D002138:Calibration; D009569:Nitric Oxide / Q000032:analysis*; D009603:Nitroso Compounds / Q000032:analysis; D012680:Sensitivity and Specificity; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000032:analysis; D014867:Water / Q000737:chemistry
D009603:Nitroso Compounds; D012996:Solutions; D013438:Sulfhydryl Compounds; D014867:Water; D009569:Nitric Oxide