Respiratory epithelial permeability in complete Freund's adjuvant rat lung granulomatosis.
To investigate Freund's adjuvant-induced changes in respiratory epithelial permeability, we determined the respiratory epithelium clearance of 99mTc-DTPA (RC-DTPA) in rats and the localization of horseradish peroxidase (HRP) across the lung epithelium. RC-DTPA was determined before and after intravenous injection of saline (control) or complete Freund's adjuvant (CFA). Although the respiratory epithelium was not disrupted, RC-DTPA was increased 15 min after injection of CFA and was still high after 3 d but had returned to normal after 7 d, after which it again increased and stayed high from Day 42 through Day 112. Cell number obtained by bronchoalveolar lavage increased from 15 min to 3 d after injection of CFA. Lung granulomatosis was observed 14 to 112 d after injection of CFA; changes in RC-DTPA correlated with changes in alveolar cells. HRP was present in bronchiolar intercellular spaces only in the CFA group. We conclude that (1) RC-DTPA increases in rat lung CFA granulomatosis 15 min to 3 and 42 to 112 d following injection, (2) the increase in RC-DTPA is not related to a rupture of epithelium but (3) is proportional to the cell influx into the lung, and (4) is possibly caused by the opening of tight junctions in small airways.
Mordelet-Dambrine
M
M
Université de Paris René Descartes, Laboratoire de Biologie et Pharmacologie des Epitheliums Respiratoires, France.
Stanislas-Leguern
G
G
Henzel
D
D
Barritault
L
L
Huchon
G
G
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004195:Disease Models, Animal; D004848:Epithelium / Q000000981:diagnostic imaging / Q000187:drug effects / Q000378:metabolism; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006099:Granuloma / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D008168:Lung / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism; D008171:Lung Diseases / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000378:metabolism*; D008297:Male; D010544:Peroxidases / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D016284:Technetium Tc 99m Pentetate; D013997:Time Factors
D005620:Freund's Adjuvant; D006735:Horseradish Peroxidase; D010544:Peroxidases; C021799:microperoxidase; D016284:Technetium Tc 99m Pentetate