Comparative evaluation of the acute effects of oxygen free radicals on myocardial contractility in anesthetized dogs with those occurring in the early stages of splanchnic artery occlusion and hemorrhagic shock.
Oxygen free radicals are cytotoxic and generated in excessive quantities during reoxygenation of ischemic organs. It has been demonstrated that oxygen free radicals impair cardiac contractile mechanisms in in vitro studies as well as depress myocardial contractility in in vivo experiments. The objectives of the present studies are to evaluate alterations in cardiac contractility and hemodynamics in two canine models of shock, namely, Wigger's model of hemorrhage and splanchnic artery occlusion (SAO) model. The data obtained in these models are comparatively evaluated with that caused by oxygen free radicals. Pentobarbital anesthetized dogs were instrumented to record blood pressure, heart rate, left ventricular pressure, (LVP & LVEDP) and LVdp/dt. Contractility index was evaluated as max dp/dt.p. In the Wigger's model, during the period of hemorrhage or after reinfusion of the shed blood despite marked variations in preload and afterload, index of contractility was not altered. Similarly, in the SAO model also, during the period of occlusion or after release, contractility index was not depressed. However, in both the models, after reinfusion of the blood (Wigger's) or after release of splanchnic arteries, there were gradual deteriorations of stroke volume, cardiac output, and arterial blood pressure. In contrast, after generation of free radicals by exogenous administration of xanthine plus xanthine oxidase, cardiac contractility was significantly depressed leading to decreases in stroke volume, cardiac output, and blood pressure. Using identical procedures to evaluate contractility, we have demonstrated that the initial depression of myocardial contractility was not the causative factor for circulatory failure in the two models of shock.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Pharmacological & Pharmaceutical Sciences, University of Houston, TX.
S M
SM
Jacinto
B S
BS
Jandhyala
D000818:Animals; D001157:Arterial Occlusive Diseases / Q000503:physiopathology*; D004285:Dogs; D005609:Free Radicals; D006439:Hemodynamics / Q000502:physiology; D009200:Myocardial Contraction / Q000502:physiology*; D010100:Oxygen / Q000502:physiology*; D010424:Pentobarbital; D012771:Shock, Hemorrhagic / Q000503:physiopathology*; D013152:Splanchnic Circulation / Q000502:physiology*; D013997:Time Factors
D005609:Free Radicals; D010424:Pentobarbital; D010100:Oxygen