Cloning of the rat edg-1 immediate-early gene: expression pattern suggests diverse functions.
The edg-1 immediate-early gene encodes a G-protein-coupled receptor homolog implicated in endothelial cell differentiation. We report the cloning of the rat edg-1 gene. Our Northern analyses indicate that edg-1 is much more widely expressed than previously thought. edg-1 mRNA was found in many organs at several stages of development with relatively high levels present in adult brain. edg-1 transcripts were also detected in several cell lines. Expression of edg-1 mRNA in the PC12 cell model of neuronal differentiation was unaffected by agents that cause PC12 cells to differentiate or proliferate. Therefore, edg-1 may play a cell-type-specific role in differentiation and also participate in neurotransmission.
Lado
D C
DC
Department of Neuroscience, University of Florida Brain Institute, University of Florida College of Medicine, Gainesville 32610.
Browe
C S
CS
Gaskin
A A
AA
Borden
J M
JM
MacLennan
A J
AJ
D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D005314:Embryonic and Fetal Development; D015870:Gene Expression; D017781:Genes, Immediate-Early; D005865:Gestational Age; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009474:Neurons / Q000502:physiology; D009928:Organ Specificity; D016716:PC12 Cells; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats / Q000235:genetics*; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D017386:Sequence Homology, Amino Acid
D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid