Antimitotic and cytotoxic flavonols from Zieridium pseudobtusifolium and Acronychia porteri.
Bioassay-guided fractionation of the extracts of Zieridium pseudobtusifolium and Acronychia porteri led to the isolation of 5,3'-dihydroxy-3,6,7,8,4'-pentamethoxyflavone [1], which showed activity against (KB) human nasopharyngeal carcinoma cells (IC50 0.04 micrograms/ml) and inhibited tubulin assembly into microtubules (IC50 12 microM). Two other known flavonols, digicitrin [2] and 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone [5], were also isolated together with three new ones, 3-O-demethyldigicitrin [3], 3,5,3'-trihydroxy-6,7,8,4'-tetramethoxyflavone [4], and 3,5-dihydroxy-6,7,8,3',4'-pentamethoxyflavone [6]. All of these flavonols showed cytotoxic activity against KB cells.
Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France.
J J
JJ
Lichius
O
O
Thoison
A
A
Montagnac
M
M
Païs
F
F
Guéritte-Voegelein
T
T
Sévenet
J P
JP
Cosson
A H
AH
Hadi
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D007624:KB Cells; D009682:Magnetic Resonance Spectroscopy; D008296:Malaysia; D013058:Mass Spectrometry; D009510:New Caledonia; D018515:Plant Leaves / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D014404:Tubulin