Characterization of three putative Serpulina hyodysenteriae hemolysins.
Serpulina hyodysenteriae hemolysin is though to be an important virulence factor in swine dysentery. One gene, tlyA, previously called tly, encoding a hemolysin in S. hyodysenteriae strain B204 has been characterized (Muir et al. Infect Immun 1992; 60: 529-35). Two other genes of S. hyodysenteria strain B204, designated tlyB and tlyC, encoding hemolytic proteins in Escherichia coli strain DH5 alpha were cloned and sequenced. The tlyB and tlyC genes, when expressed in E. coli, encode heat-labile, protease-sensitive proteins which exhibit both hemolytic and cytotoxic activity in vitro. The calculated molecular weights of the tlyB and tlyC gene products are 93.3 kDa and 30.8 kDa, respectively. The tlyB gene product has sequence homology with the Clp proteins, whereas for the tlyC-encoded protein no homology with other protein sequences was observed. Southern hybridization showed that the tlyB and tlyC genes are present in a single copy on the chromosome of S. hyodysenteriae.
ter Huurne
A A
AA
Department of Bacteriology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Muir
S
S
van Houten
M
M
van der Zeijst
B A
BA
Gaastra
W
W
Kusters
J G
JG
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D017400:Brachyspira / Q000235:genetics; D017401:Brachyspira hyodysenteriae / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D018628:Gene Dosage; D005798:Genes, Bacterial / Q000235:genetics*; D015698:Genomic Library; D006367:HeLa Cells; D006460:Hemolysin Proteins / Q000235:genetics* / Q000633:toxicity; D006462:Hemolytic Plaque Technique; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000633:toxicity; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C090673:TlyB protein, Brachyspira hyodysenteriae; C090674:TlyC protein, Brachyspira hyodysenteriae