Direct determination of the enantiomers of the antimalarial drug halofantrine and its active metabolite N-desbutylhalofantrine in human plasma.
The plasma concentrations vs. time profiles of the enantiomers of halofantrine and its main metabolite N-desbutylhalofantrine have been determined in two healthy volunteers after a single oral administration of 500 mg of racemic halofantrine hydrochloride (Halfan). A solid-phase extraction method was used for the purification of the samples. The enantiomers of halofantrine and N-desbutylhalofantrine were determined simultaneously using chiral high-performance liquid chromatography on chiral stationary phases with fluorescence detection.
Terefe
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H
Institute of Pharmaceutical Chemistry, University of Münster, Germany.
Blaschke
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G
D000328:Adult; D000962:Antimalarials / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010616:Phenanthrenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D000962:Antimalarials; D010616:Phenanthrenes; C044961:1,3-dichloro-6-trifluoromethyl-9-phenanthryl-3-(n-butyl)aminopropanol; C023768:halofantrine