Plasmenylethanolamine in human intestinal mucosa detected by an improved method for analysis of phospholipid.
Analysis of phospholipid and their fatty acid composition of human intestinal mucosa was performed by an method elaborated to analyze the limited amount of sample with 2-dimensional TLC followed by lipid-phosphorus determination. Using this method, plasmenylethanolamine was detected in human intestinal mucosa and accounted for about 7% of phospholipid in small and large intestinal mucosa. The amounts of polyunsaturated fatty acids of phosphatidylethanolamine were higher than those of other phosphoglycerides in intestinal mucosa, hence, inflammation-related eicosanoids may originate from ethanolamine containing phospholipid.
Division of Biochemistry and Nutrition, International Medical Center of Japan, Tokyo.
K
K
Nakanishi
E
E
Yasugi
H
H
Morita
T
T
Dohi
M
M
Oshima
D016718:Arachidonic Acid / Q000032:analysis; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer / Q000379:methods; D003106:Colon / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry*; D010743:Phospholipids / Q000378:metabolism; D010955:Plasmalogens / Q000032:analysis*; D012680:Sensitivity and Specificity
D005227:Fatty Acids; D010743:Phospholipids; D010955:Plasmalogens; C020791:phosphatidal ethanolamines; D016718:Arachidonic Acid