Liver lipid peroxidation and glutathione-related defence enzyme systems in mice treated with paracetamol.
Glutathione levels were found to be decreased while lipid peroxide levels were increased in total liver homogenates 6 h following paracetamol treatment (500 mg kg-1 i.p.). Furthermore, it has been determined that cytosolic glutathione S-transferase (GST) activity was decreased and glutathione peroxidase (GSH-Px) activity remained unchanged. On the other hand, a decrease in liver microsomal lipid peroxide levels and an increase in GST and GSH-Px activity has been observed. We concluded that decreased lipid peroxide levels in microsomes could be a consequence of increased GSH-Px and GST enzyme activities. In this way, these glutathione-related defence enzyme systems may play an important role in protecting microsomes from lipid peroxidation.
Department of Biochemistry, Istanbul Faculty of Medicine, University of Istanbul, Capa, Turkey.
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Ozdemirler
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Aykaç
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Uysal
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Oz
D000082:Acetaminophen / Q000633:toxicity*; D000818:Animals; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology
D000082:Acetaminophen; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione