The suppressor of hairless protein participates in notch receptor signaling.
In a genetic screen for mutations that attenuate Notch signaling in the developing Drosophila eye, we isolated rare, gain-of-function alleles of Suppressor of Hairless (Su(H)), the fly homolog of the mammalian C promoter-binding factor 1 (CBF1) gene. Su(H) exhibits numerous allele-specific genetic interactions with Notch as well as with Delta, deltex, and mastermind. In cultured Drosophila cells, the Su(H) protein is sequestered in the cytoplasm when coexpressed with Notch protein and is translocated to the nucleus when Notch binds to its ligand Delta. Cytoplasmic retention of Su(H) requires the intracellular cdc10/ankyrin repeats of Notch, which associate with Su(H) protein in the yeast interaction trap assay. These results indicate that Notch activity may regulate nuclear events by controlling the activity of a DNA-binding protein.
Fortini
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ME
Howard Hughes Medical Institute, Department of Biology, Yale University, New Haven, Connecticut 06536.
Artavanis-Tsakonas
S
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D000595:Amino Acid Sequence; D000818:Animals; D017487:Ankyrins / Q000737:chemistry; D002451:Cell Compartmentation; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins / Q000502:physiology; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000502:physiology*; D005656:Fungal Proteins / Q000737:chemistry; D017344:Genes, Insect; D007301:Insect Hormones / Q000737:chemistry / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000737:chemistry / Q000502:physiology*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009687:Nuclear Proteins / Q000502:physiology; D011485:Protein Binding; D051880:Receptors, Notch; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins; D015398:Signal Transduction; D014157:Transcription Factors
D017487:Ankyrins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C086679:DX protein, Drosophila; D029721:Drosophila Proteins; D005656:Fungal Proteins; D007301:Insect Hormones; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; C497759:N protein, Drosophila; D009687:Nuclear Proteins; D051880:Receptors, Notch; D012097:Repressor Proteins; D029702:Schizosaccharomyces pombe Proteins; C088731:Su(H) protein, Drosophila; D014157:Transcription Factors; C061178:cdc10 protein, S pombe; C064045:delta protein