A highly efficient technique for cloning of refractory DNA fragments and polymerase chain reaction products.
Parent
J L
JL
Department of Pediatrics, Faculty of Medicine, Université de Sherbrooke, Quebec, Canada.
Le Gouill
C
C
Rola-Pleszczynski
M
M
Stanková
J
J
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D015183:Restriction Mapping; D019914:Taq Polymerase
D017931:DNA Primers; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D004247:DNA; C072146:Pfu DNA polymerase; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase