Single- and double-strand breaks in pBR322 DNA by vacuum-UV from 8.3 to 20.7 eV.
Solid pBR322 DNA was irradiated in a vacuum by monochromatic photons from 8.3 eV (150 nm) to 20.7 eV (60 nm), and the formation of single- and double-strand breaks (ssb and dsb) was determined using agarose gel electrophoresis. The action cross sections increased 20 times (ssb) and 43 times (dsb) from 8.3 eV to 20.7 eV; the quantum yields increased 5.4 times (ssb) and 12 times (dsb). The cross sections for dsb were 0.0059 at 8.3 eV and 0.013 at 20.7 eV of those of ssb; these values were smaller than about 0.04 for 2.1 keV photons, or about 0.18 for 60Co gamma-rays. Although vacuum UV photons were proved to induce dsb, they had a lower efficiency than when using soft X-rays or gamma-rays.
Biophysics Laboratory, College of Science, Rikkyo (St. Paul's) University, Tokyo, Japan.
K
K
Hieda
K
K
Suzuki
T
T
Hirono
M
M
Suzuki
Y
Y
Furusawa
D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004587:Electrophoresis, Agar Gel; D011829:Radiation Dosage; D014466:Ultraviolet Rays / Q000009:adverse effects; D014618:Vacuum
D004277:DNA, Single-Stranded; D004247:DNA