Effect of myosin B on guinea pig muscles--light microscopic and immunological aspects.
Experimental myositis was induced in guinea pigs by multiple inoculations with Myosin B fraction of the rabbit skeletal muscle. Quantitative histopathological features were studied and they include necrosis, central nucleation, inflammatory cellular reaction. Besides myositis, features of myocarditis were evident in 40% animals. Circulating immune complexes (CICs) in the sera of experimental animals were isolated by a polyethylene glycol (PEG) precipitation method. Presence of antimyosin B antibody in the immune complexes was characterised by an enzyme-linked immunosorbent assay. The role of circulating immune complexes in pathogenesis of experimental myositis and human polymyositis have been emphasised.
Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
V V
VV
Krishnan
A
A
Saraswathy
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D006168:Guinea Pigs; D007114:Immunization; D008297:Male; D008853:Microscopy / Q000379:methods; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D009218:Myosins / Q000494:pharmacology*; D009220:Myositis / Q000276:immunology*
D000936:Antigen-Antibody Complex; D009218:Myosins