Human lymphoblastoid interferon as vaccine adjuvant in cattle.
Human lymphoblastoid interferon from Namalwa cells was purified for clinical use by ethanol fractionation, and used as adjuvant of an inactivated Bovid Herpesvirus 1 vaccine in calves. In agreement with other in vitro and in vivo models, low and high interferon doses were shown to increase and depress the specific antibody response, respectively. The low, effective interferon dose (100 International Units/kg) also reduced the variability of antibody titres after the first vaccine injection. This latter dose had apparently no influence on the regulatory T cell circuits, as opposed to the other doses under study.
Department of Vaccine Research and Development Instituto Zooprofilattico Sperimentale Lombardia ed Emilia, Brescia, Italy.
M
M
Amadori
I L
IL
Archetti
C
C
Berneri
E
E
Lodetti
M
M
Salvalai
P
P
Cordioli
S
S
Callegari
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D004306:Dose-Response Relationship, Immunologic; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000008:administration & dosage*; D007371:Interferon-gamma / Q000378:metabolism; D008213:Lymphocyte Activation; D015164:Vaccines, Inactivated / Q000008:administration & dosage; D014765:Viral Vaccines / Q000008:administration & dosage*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D016898:Interferon-alpha; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D007371:Interferon-gamma