Purification and properties of six aldo-keto reductases from rat adrenal gland.
Six aldo-keto reductases from rat adrenal gland have been highly purified to apparent homogeneity. These enzymes were identified as carbonyl reductases (CR-K1, CR-K2, CR-A, and CR-B), aldehyde reductase (AR-H), and aldose reductase (AR-L) in terms of substrate specificity, molecular weight (33,000-39,000), inhibitor susceptibility, cofactor requirement, and immunochemical properties. Both CR-K1 and CR-K2 were characterized as possessing high affinity towards 13,14-dihydro-15-ketoprostaglandin F2 alpha and were localized immunohistochemically in the zona glomerulosa and the zona reticularis of adrenal cortex, and in the ganglion cell of adrenal medulla. Immunoreactive proteins to anti-CR-K2 antibody were observed in male and female reproductive tissues of rats. Positive immunoreactive protein to anti-CR-A antibody was found in mouse, hamster, and rabbit adrenal gland, whereas that to anti-CR-K2 antibody was present only in rat adrenal gland. AR-H and AR-L mainly reduced aromatic and aliphatic aldehydes. All the aldo-keto reductases from rat adrenal gland were completely inhibited by p-chloromercuribenzoate. Barbiturate and 3,3'-tetramethylene glutarate potently inhibited AR-H, and quercitrin significantly decreased the activity of CR-K1, CR-K2, and AR-L. We propose that these aldo-keto reductases may play important roles in the rat adrenal functions.
Department of Pharmacology, Teikyo University School of Medicine, Tokyo.
N
N
Inazu
Y
Y
Nagashima
T
T
Satoh
T
T
Fujii
D000302:Adrenal Cortex / Q000201:enzymology*; D000313:Adrenal Medulla / Q000201:enzymology*; D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification; D000074408:Aldo-Keto Reductases; D000818:Animals; D003429:Cross Reactions; D005260:Female; D006863:Hydrogen-Ion Concentration; D007120:Immunochemistry; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification*; D008297:Male; D051381:Rats; D011921:Rats, Inbred WKY; D013379:Substrate Specificity
D007527:Isoenzymes; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; C019642:alcohol dehydrogenase (NADP+); D000449:Aldehyde Reductase; C000614209:AKR7A5 protein, mouse