Antioxidant supplementation partially protects against myocardial mitochondrial ischemia/reperfusion injury, but ascorbate in the perfusate prevented the beneficial effect.
This study was undertaken to investigate whether prior antioxidant supplementation had a beneficial effect on subsequent myocardial ischemic/reperfusion injury and whether addition of ascorbate during ischemia/reperfusion had any effect. Supplementation with antioxidants resulted in elevated concentrations of myocardial alpha-tocopherol, but not of ascorbate. Combined supplementation with alpha-tocopherol, beta-carotene and ascorbic acid gave the highest myocardial alpha-tocopherol concentration. Hearts of rats supplemented with antioxidants was partially protected to ischemia/reperfusion as indicated by the mitochondrial function. However, addition of ascorbate during ischemia/reperfusion nullified this protective effect.
Department of Chemical Pathology (G3), University of the Orange Free State, Bloemfontein, Republic of South Africa.
H
H
van Jaarsveld
J M
JM
Kuyl
D W
DW
Alberts
M N
MN
Wiid
D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D005260:Female; D007501:Iron / Q000032:analysis; D008929:Mitochondria, Heart / Q000502:physiology*; D017202:Myocardial Ischemia / Q000503:physiopathology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D009206:Myocardium / Q000737:chemistry; D010084:Oxidation-Reduction; D010477:Perfusion; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000032:analysis
D000975:Antioxidants; D014810:Vitamin E; D007501:Iron; D001205:Ascorbic Acid