Automated microanalysis of oxcarbazepine and its monohydroxy and transdiol metabolites in plasma by liquid chromatography.
An automated high-performance liquid chromatographic method for the simultaneous determination of oxcarbazepine and its monohydroxy and transdiol metabolites in plasma is described. 5,6-Dihydro-11-oxo-11H-dibenz[b,e]azepine-5-carboxamide was used as internal standard. Liquid-solid extraction from plasma (100 microliters) on 50 mg Bond-Elut C18 cartridges was automatically performed by the Automatic Sample Preparation with Extraction Columns (ASPEC) system. A reversed-phase column (ODS Hypersil, 3 microns particle size, 4 cm x 4.6 mm I.D.) was used with acetonitrile-methanol-0.01 M potassium dihydrogenphosphate as mobile phase. The eluted compounds were detected at 210 nm. The limit of quantitation was 0.2 mumol/l for oxcarbazepine and 0.1 mumol/l for its metabolites. No interference with concomitantly administered anti-epileptic drugs such as phenobarbital, phenytoin, valproic acid or carbamazepine, was found.
Rouan
M C
MC
Laboratoires Ciba-Geigy, Biopharmaceutical Research Center, Rueil-Malmaison, France.
Decherf
M
M
Le Clanche
V
V
Lecaillon
J B
JB
Godbillon
J
J
D000927:Anticonvulsants / Q000097:blood*; D001322:Autoanalysis; D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D008832:Microchemistry; D000078330:Oxcarbazepine; D011786:Quality Control; D012680:Sensitivity and Specificity
D000927:Anticonvulsants; D002220:Carbamazepine; D000078330:Oxcarbazepine