A mouse cDNA encoding a protein with zinc-fingers and a KRAB domain shows similarity to human profilaggrin.
As part of our efforts to understand transcriptional regulation in the vertebrate eye lens, we have isolated a clone that encodes a zinc finger (Zf) protein from a newborn mouse lens cDNA library. Corresponding message for this protein is detectable in the lens, liver, heart, kidney, spleen and brain of newborn mice. A longer cDNA containing the complete ORF for the same protein was isolated from an adult mouse testis library. A conceptual translation of the testis cDNA sequence produces a 555-amino-acid (aa) protein that contains nine C-terminal Zf and an N-terminal domain found in a subset of C2H2 Zf, the Krüppel-associated box (KRAB). The aa sequence located between the KRAB domain and the Zf shows an unexpected similarity to human profilaggrin, a protein expressed in differentiating epidermal cells. Sequences that hybridize to this cDNA are detectable in ten other mammalian species.
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
J P
JP
Brady
J
J
Piatigorsky
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D015139:Blotting, Southern; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D000091344:Filaggrin Proteins; D015723:Gene Library; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics*; D007668:Kidney / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors; D007908:Lens, Crystalline / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008322:Mammals; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D016366:Open Reading Frames; D009928:Organ Specificity; D010750:Phosphoproteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D051741:Kruppel-Like Transcription Factors; D010750:Phosphoproteins; D011498:Protein Precursors; D012097:Repressor Proteins; D014157:Transcription Factors