The role of K+ influx on glutamate induced astrocyte swelling: effect of temperature.
We studied factors involved in glutamate-stimulated astrocyte swelling and the role of temperature in this process. Glutamate-induced astrocyte swelling was reduced by 74% when temperature was lowered from 37 degrees C to 24 degrees C. Reduction in temperature to 24 degrees C also resulted in inhibition of glutamate-stimulated K+ uptake. When both extracellular and intracellular Ca2+ were removed with BAP-TA/AM (50 microM) and 0.1 mM EGTA and in the absence of extracellular Ca2+, glutamate was not able to stimulate the K+ uptake or swelling. Dantrolene (20 microM), MK-801 (0.5 mM) and ouabain (0.5 mM) inhibited glutamate-induced K+ uptake as well as the glutamate effects on swelling. These findings show the importance of Ca2+ in the mechanism of glutamate swelling and suggests that glutamate induces swelling through stimulation of K+ influx and that the diminished effect of glutamate swelling with decrease in temperature may be due to a decrease in K+ uptake.
Bender
A S
AS
Department of Pathology, University of Miami School of Medicine, Florida.
Norenberg
M D
MD
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000187:drug effects* / Q000502:physiology; D002118:Calcium / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D005110:Extracellular Space / Q000187:drug effects / Q000502:physiology; D007424:Intracellular Fluid / Q000187:drug effects* / Q000502:physiology; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D013696:Temperature; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology
D015221:Potassium Channels; D011188:Potassium; D002118:Calcium