Computerized in vitro test for chemical toxicity based on Tetrahymena swimming patterns.
An apparatus and a method for rapidly determining chemical toxicity have been evaluated as an alternative to the rabbit eye initancy test (Draize). The toxicity monitor includes an automated scoring of how motile biological cells (Tetrahymena pyriformis) slow down or otherwise change their swimming patterns in a hostile chemical environment. The method, called the Motility Assay (MA), is tested for 30 s to determine the chemical toxicity in 20 aqueous samples containing trace organics and salts. With equal or better detection limits, results compare favorably to in vivo animal tests of eye irritancy.
Noever
D A
DA
NASA Marshall Space Flight Center, Huntsville, AL 35812.
Matsos
H C
HC
Cronise
R J
RJ
Looger
L L
LL
Relwani
R A
RA
Johnson
J U
JU
D000826:Animal Testing Alternatives; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D005123:Eye / Q000187:drug effects; D007509:Irritants / Q000633:toxicity*; D011817:Rabbits; D013769:Tetrahymena pyriformis / Q000187:drug effects* / Q000502:physiology; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D007509:Irritants; D014874:Water Pollutants, Chemical