Effect of sulfite intake on intestinal enzyme activity in rats.
Sulfites are usually added to food, beverages and pharmaceuticals as preservative antioxidants, bleaching agents, and dough conditioning agents. Ingestion of foods containing sulfites can cause abdominal pain, diarrhoea, seizures and death. Sulfite can react with cellular components and can cause toxicity. Changes in mucosal disaccharidases and phosphatase alkaline after sodium metabisulfite administration were investigated in the small intestine of rats. Female Wistar rats were given a diet supplemented with 0.25 or 2.5% sodium metabisulfite for 5 weeks. Sucrase, maltase, lactase and alkaline phosphatase were assayed in intestinal homogenates and in brush border membrane fractions. The intake of only 2.5% sulfite induced an increase in the specific activities of sucrase, maltase, and alkaline phosphatase compared to control levels (P < 0.05). Lactase levels were affected in a variable manner. The origin of such altered enzyme activities is still unknown.
Rodriguez Vieytes
M
M
Departamento de Fisiologia, Facultad de Farmacia, Universidad de Santiago de Compostela, España.
Martinez-Sapiña
J
J
Taboada Montero
C
C
Lamas Aneiros
M
M
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D005260:Female; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013393:Sucrase / Q000378:metabolism*; D013447:Sulfites / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
D013447:Sulfites; C030981:metabisulfite; D000469:Alkaline Phosphatase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D013393:Sucrase