A square signal wave method for measurement of brain extra- and intracellular water content.
Brain tissue electrical impedance is a commonly used method to evaluate the dynamics of brain edema. We have found the square wave impedance method simpler and more cost-effective than the currently used sine wave impedance method. This square wave method avoids the necessity for expensive frequency control and amplitude-phase measuring devices as well as simplifying on-line data processing. In our experiments the electrical impulse was generated by a pulse generator of Macintosh data acquisition system. The signal (I = 11 muA, t = 2-20 ms) was delivered every 2-3 s external electrodes of a tetrapolar system through a specially designed isolation-calibration device. This electrode system was inserted into the cerebral cortex of experimental animals (rat). The cerebral cortex was found to have linear electrical properties in the 5-30 muA range. Our impedance measurement system was tested in calibration trials, and showed system reliability and accuracy. The system was also tested in pilot experiments, in vivo, in a rat brain osmotic edema model.
Itkis
M L
ML
Aitken Neurosurgery Laboratory, Department of Surgery, Cornell University Medical College, New York, NY.
Roberts
J K
JK
Ghajar
J B
JB
Hariri
R J
RJ
D000818:Animals; D001929:Brain Edema / Q000503:physiopathology*; D002138:Calibration; D002540:Cerebral Cortex / Q000503:physiopathology*; D017097:Electric Impedance; D005110:Extracellular Space / Q000502:physiology*; D007424:Intracellular Fluid / Q000502:physiology*; D051381:Rats; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D012984:Software