Determination of ascorbic acid and uric acid in plasma by high-performance liquid chromatography.
A simple method with excellent reproducibility has been developed for the simultaneous determination of the plasma concentration of ascorbic acid and uric acid by reversed-phase HPLC using an ion-pairing reagent with UV detection. Coefficients of variation for the concentrations of ascorbic acid and uric acid in plasma are < 2.1% and < 3.5% respectively.
Rowett Research Institute, Bucksburn, Aberdeen, UK.
M A
MA
Ross
D001205:Ascorbic Acid / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014527:Uric Acid / Q000097:blood*
D014527:Uric Acid; D001205:Ascorbic Acid