Correlation between the in vitro and in vivo effects of fourteen beta-lactam compounds in mice with systemic infection.
The in vivo effect of 14 beta-lactam compounds was compared with the in vitro effect in mice systemically infected with Pseudomonas aeruginosa E7 or Klebsiella pneumoniae 3K-25. The microorganisms were inoculated intraperitoneally, and the in vivo effect of the drug was expressed as the median effective dose (ED50) determined by subcutaneous administration of the drug 1 h after the inoculation. In the mice infected with P. aeruginosa, the ability of the drug to kill the bacteria in a short time (killing activity) was markedly correlated with the ED50 (r = -0.704), but there was no correlation with the maximum concentration, half-life or area under the curve of the drug. The coefficient of multiple correlation of the ED50 with killing activity+time above the minimum bactericidal concentration (MBC) was 0.824 (contribution rate, 67.9%). In the mice infected with K. pneumoniae, the killing activity was highly correlated with MBC (r = 0.899), and the coefficient of multiple correlations with MBC+time above MBC was 0.919 (contribution rate, 84.4%). These results suggest that beta-lactam compounds have strong bactericidal activity in mice with systemic infection and that drugs with a long time above MBC are effective for this condition.
Tsuji
A
A
Department of Microbiology, Toho University School of Medicine, Tokyo, Japan.
Kaneko
Y
Y
Yamaguchi
K
K
Goto
S
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy*; D007711:Klebsiella pneumoniae / Q000187:drug effects*; D051379:Mice; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013045:Species Specificity; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams