High incidence of H-ras oncogene mutations in squamous cell carcinoma of lip vermilion.
Nine specimens of well-differentiated squamous cell carcinoma of the lip vermilion have been analyzed for the presence of H-ras oncogene mutations, using the technique of hybridization with synthetic oligonucleotide probes on in vitro amplified tumour DNA. Five specimens harbored mutations: four in codon 12 and one in codon 13. This high incidence (55%) of mutated H-ras genes suggests that their activation may play an important role in lip tumour development and may be connected to the exposure to chemical and/or physical carcinogens.
J
J
Milasin
N
N
Pujić
N
N
Dedović
Z
Z
Nikolić
V
V
Petrović
B
B
Dimitrijević
D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D005998:Glycine / Q000235:genetics; D006801:Humans; D008048:Lip Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D017354:Point Mutation; D016133:Polymerase Chain Reaction
D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D005998:Glycine