Physiochemical and immunological characterization of hepatitis A virus nucleocapsids expressed in a vaccinia virus/T7/EMCV system.
Expression of the entire open reading frame of hepatitis A virus was achieved in a vaccinia virus/T7/EMCV hybrid system. The expressed antigens were characterized as particles with buoyant densities and sedimentation coefficients typical of empty capsids and pentamers of HAV. This was further confirmed by electron microscopy. All capsid proteins were determined as components of the particles which raised neutralizing antibodies in an immunized rabbit.
Zhu
N L
NL
Shanghai Institute of Biochemistry, Academia Sinica, People's Republic of China.
Li
G D
GD
Wang
Y
Y
D000818:Animals; D002213:Capsid / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004686:Encephalomyocarditis virus / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D006168:Guinea Pigs / Q000276:immunology; D006507:Hepatovirus / Q000276:immunology / Q000378:metabolism*; D015151:Immunoblotting; D008854:Microscopy, Electron; D009500:Neutralization Tests; D011817:Rabbits / Q000276:immunology; D014616:Vaccinia virus / Q000378:metabolism; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology
D014758:Viral Core Proteins