Characterization of the DNA-binding state of the rat uterine estrogen receptor by UV cross-linking.
Characterization of the DNA-binding state of the rat uterine estrogen receptor was examined by UV cross-linking using [32P]5-bromo-2'-deoxyuridine-substituted 25-base pair synthetic oligonucleotide containing a Xenopus vitellogenin A2 estrogen response element (BrdUVRE). After UV irradiation of the receptor-BrdUVRE complexes, they were analyzed by SDS-polyacrylamide gel electrophoresis. In the nuclear estradiol-, ICI164, 384- and 4-hydroxytamoxifen-receptor complexes, the bands corresponding to the mobility of the receptor homodimer were observed. In a molybdate stabilized soluble receptor, we observed the bands with the slower mobility than the receptor homodimers.
Department of Biochemistry, Kawasaki Medical School, Okayama, Japan.
M
M
Ikeda
T
T
Kusaka
D000818:Animals; D001483:Base Sequence; D001973:Bromodeoxyuridine / Q000378:metabolism; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects*; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000528:radiation effects*; D004586:Electrophoresis; D005260:Female; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000494:pharmacology; D010761:Phosphorus Radioisotopes; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000378:metabolism* / Q000528:radiation effects*; D012995:Solubility; D013379:Substrate Specificity; D014466:Ultraviolet Rays; D014599:Uterus / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure*; D014819:Vitellogenins / Q000378:metabolism; D014981:Xenopus
D004268:DNA-Binding Proteins; D010761:Phosphorus Radioisotopes; D011960:Receptors, Estrogen; D014819:Vitellogenins; C044659:molybdate; D008982:Molybdenum; D004247:DNA; D001973:Bromodeoxyuridine