Olfactory ensheathing glia and platelet-derived growth factor B-chain reactivity in the transplanted rat olfactory bulb.
Using a monoclonal antibody against the B-chain of platelet-derived growth factor as a marker, we have examined the behavior of olfactory ensheathing glia in the normal and transplanted rat olfactory bulb. In the normal postnatal olfactory bulb, these glia are found to ensheath the bundles of incoming primary olfactory nerve fibers as well as those in the olfactory nerve layer. Olfactory marker protein antibody was used to identify the olfactory nerve proper. Within the transplant, the same glia: (1) ensheath bundles of both primary olfactory and non-primary olfactory axons, (2) ensheath axonal bundles deep within the donor tissue, and (3) eventually permit radiation of individual axons from bundles to surrounding neuropil. We believe that ensheathing glia (being rich in growth-related factors and extracellular matrix molecules) may be useful in providing trophic support and guidance for the reconstruction of developmentally or traumatically damaged neuronal pathways not directly related to the olfactory system. The evidence presented here indicates that ensheathing glia are capable of existing in deep brain areas and ensheathing other than primary olfactory axons. The special molecular characteristics of these glia along with the morphological findings presented here provide a foundation for further studies of these unique glia and their potential utility in the restoration of damaged neural pathways.
Department of Neurological Surgery, University of Washington, Seattle 98195.
J N
JN
Kott
L E
LE
Westrum
E W
EW
Raines
M
M
Sasahara
R
R
Ross
D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D016380:Brain Tissue Transplantation / Q000502:physiology*; D005260:Female; D016332:Fetal Tissue Transplantation / Q000502:physiology*; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology* / Q000378:metabolism* / Q000637:transplantation; D051842:Olfactory Marker Protein; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D009419:Nerve Tissue Proteins; D051842:Olfactory Marker Protein; C497599:Omp protein, rat; D010982:Platelet-Derived Growth Factor