Erythrocyte catalase inactivation (H2O2 production) by ascorbic acid and glucose in the presence of aminotriazole: role of transition metals and relevance to diabetes.
Erythrocytes exposed to ascorbic acid in the presence of aminotriazole undergo a dose- and time-dependent inactivation of endogenous catalase which is proportional to environmental hydrogen peroxide (H2O2) concentrations. The production of H2O2 seems to be dependent upon the availability of transition metal chelatable by o-phenanthroline (OPT), although the kinetics of catalase inactivation and H2O2 production by externally added copper ions in the presence of OPT is complex. Furthermore, although glucose is also able to undergo a transition-metal-catalysed oxidation yielding H2O2, the production of H2O2 by glucose seems to be a minor process by comparison with ascorbic acid oxidation. Indeed, on the basis of these data, transition-metal-catalysed ascorbic acid oxidation is likely to be a more important source of oxidative stress in the diabetic state than hyperglycaemia.
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Department of Medicine, University College, London, U.K.
Wolff
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SP
D000640:Amitrole / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002374:Catalase / Q000037:antagonists & inhibitors*; D003920:Diabetes Mellitus / Q000097:blood* / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007700:Kinetics; D008670:Metals / Q000378:metabolism*; D018384:Oxidative Stress
D008670:Metals; D006861:Hydrogen Peroxide; D002374:Catalase; D005947:Glucose; D001205:Ascorbic Acid; D000640:Amitrole