Effect of beta-carotene, sodium ascorbate and cellulose on 1,2-dimethylhydrazine-induced intestinal carcinogenesis in rats.
Male Sprague-Dawley rats were fed a diet containing either 0.005% beta-carotene, 0.02% sodium ascorbate or 1.5% cellulose for 14 weeks. Beginning on day 3, all animals were also given weekly subcutaneous injections of 1,2-dimethylhydrazine (DMH; 20 mg/kg body wt.) throughout a 12-week period. The experimental diet was continued for an additional 14 weeks. At the end of the 26th week, surviving animals were sacrificed and the incidence of intestinal carcinomas was examined. A significantly lower incidence of carcinomas was observed in the beta-carotene-fed group (55.0%), compared with that in the control group given DMH (82.1%).
Department of Pathology, Kitasato University School of Hygienic Sciences, Kanagawa, Japan.
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Yamamoto
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Maruyama
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Moriguchi
D019813:1,2-Dimethylhydrazine; D000818:Animals; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002338:Carotenoids / Q000627:therapeutic use*; D002482:Cellulose / Q000627:therapeutic use*; D004127:Dimethylhydrazines / Q000633:toxicity*; D007414:Intestinal Neoplasms / Q000139:chemically induced* / Q000517:prevention & control*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D019207:beta Carotene
D016588:Anticarcinogenic Agents; D004127:Dimethylhydrazines; D019207:beta Carotene; D002338:Carotenoids; D002482:Cellulose; D019813:1,2-Dimethylhydrazine; D001205:Ascorbic Acid