[A standardized AgNOR stain method for formalin fixed and paraffin embedded material].
Visualization of proteins associated with nucleolar organizer regions proteins (AgNORs) on formalin-fixed and paraffin-embedded archival tissues is substantially improved after application of wet autoclave pretreatment. Silver staining results are comparable to those obtained on tissues processed in alcohol based fixatives, illustrating AgNORs as substructures of the nucleoli without any staining artefacts. A highly reproducible staining quality was achieved irrespective of tissue origin or duration of formalin fixation. As a result of this novel and simple method, the grounds have been prepared for standardized AgNOR quantification on archival material.
Gerhard-Domagk-Institut für Pathologie, Westfälische Wilhelms-Universität, Münster.
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Ofner
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Bankfalvi
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Riehemann
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Böcker
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Schmid
D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D009369:Neoplasms / Q000648:ultrastructure*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D016612:Paraffin Embedding; D015203:Reproducibility of Results; D016622:Silver Staining
D005404:Fixatives; D005557:Formaldehyde
Standardisierte AgNOR-Färbemethode für formalinfixiertes und paraffineingebettetes Material.