Sensitivity of fructose-1,6-bisphosphatase to glucose and cyclic AMP in the fission yeast Schizosaccharomyces pombe.
Stationary phase cells from resting cultures of Schizosaccharomyces pombe, suspended in buffer, did not decrease fructose-1,6-bisphosphatase (FbPase) activity upon addition of glucose to the cell suspension. In contrast, the addition of glucose to cells from derepressed growing cultures resulted in a 4- to 5-fold reduction in FbPase activity within minutes after the addition of the sugar. This response was independent of protein synthesis, was accompanied by a rise in the cyclic AMP (cAMP) level and was concomitant with an increase in the activity of neutral trehalase. The addition of exogenous cAMP to these cells provoked a decrease in FbPase similar to that induced by glucose. The occurrence of a glucose-induced cAMP signal was not sufficient to trigger the decrease in FbPase activity, which appeared to require additional transduction elements not active in growth-arrested cells. In assays performed in vitro it was found that the enzyme activity was strongly inhibited by fructose-2,6-bisphosphate.
Carrillo
D
D
Department of Genetics and Microbiology, Facultad de Biología, University of Murcia, Spain.
Vicente-Soler
J
J
Gacto
M
M
D003470:Culture Media / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D005947:Glucose / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D014198:Trehalase / Q000096:biosynthesis
D003470:Culture Media; D005656:Fungal Proteins; D000242:Cyclic AMP; D006597:Fructose-Bisphosphatase; D014198:Trehalase; D005947:Glucose