Improvement of penicillin G acylase production by using pac gene clone.
The penicillin G acylase gene (pac gene) from Escherichia coli ATCC 9637 has been isolated. It conferred the production of penicillin G acylase upon E. coli HB101 and other enteric bacilli. Restriction enzyme analysis and subcloning studies reveal that the gene is contained within a 2.3 kb HindIII-SmaI DNA fragment. In vitro protein synthesis study suggests a gene product of approximately 90 kDa. By using the genetically engineered bacteria which harbor a novel recombinant plasmid (pGL5) bearing a constitutively expressible pac gene, a 20-fold increased production yield of penicillin G acylase activity was obtained as compared with that produced by the original strain, E. coli ATCC 9637.
Institute of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Y T
YT
Liu
D D
DD
Ji
C C
CC
Chou
H Y
HY
Chao
C L
CL
Liao
S H
SH
Han
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D010399:Penicillin Amidase / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; D010399:Penicillin Amidase