Alpha-ketoglutarate dehydrogenase in the in vitro-grown Mycobacterium lepraemurium.
The Hawaiian and Kumato strains of Mycobacterium lepraemurium were cultivated on Ogawa egg-yolk medium, and the alpha-ketoglutarate dehydrogenase activity was investigated in cell-free preparations of this mycobacterium. The enzymatic activity was mainly localized in the particulate fraction (150,000 x g pellet), and extremely low activity was found in the soluble fraction (150,000 x g supernatant). alpha-Ketoglutarate dehydrogenase was not stable; the activity was lost completely when the enzyme was kept at 45 degrees C for 1 hr or stored at -70 degrees C. The enzyme reduced only NAD+ but not NADP+ by alpha-ketoglutarate, indicating the presence of NAD(+)-dependent alpha-ketoglutarate dehydrogenase in cultivated M. lepraemurium.
Applied Microbiology Research Center, Institut Armand-Frappier, University of Quebec, Laval, Canada.
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Ishaque
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Togola
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Sticht-Groh
D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007655:Ketoglutarate Dehydrogenase Complex / Q000302:isolation & purification / Q000378:metabolism*; D009167:Mycobacterium lepraemurium / Q000201:enzymology* / Q000254:growth & development; D009243:NAD / Q000378:metabolism; D013696:Temperature
D003470:Culture Media; D009243:NAD; D007655:Ketoglutarate Dehydrogenase Complex