Shot-gun sequencing strategy for long-range genome mapping: a pilot study.
We have recently proposed a strategy for construction of long-range physical maps based on random sequencing of NotI linking and jumping clones. Here, we present results of sequence comparison between 168 NotI linking (100 of them were sequenced from both sides) and 81 chromosome 3-specific jumping clones. We were able to identify 14 NotI jumping clones (17%), each joined with two NotI linking clones. The average size of chromosomal jumps was about 650 kb. The assembled 42 NotI genomic fragments correspond to 12-15% of chromosome 3. These results demonstrate the value of random sequencing of NotI linking and jumping clones for genome mapping. This mapping proposal can be used for connecting physical and genetic maps of the human genome and will be a valuable supplement to YAC and cosmid library based mapping projects.
Zabarovsky
E R
ER
Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
Kashuba
V I
VI
Pettersson
B
B
Petrov
N
N
Zakharyev
V
V
Gizatullin
R
R
Lebedeva
T
T
Bannikov
V
V
Pokrovskaya
E S
ES
Zabarovska
V I
VI
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D016386:Chromosome Walking; D003001:Cloning, Molecular; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; D015723:Gene Library; D008040:Genetic Linkage; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D010865:Pilot Projects; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases