Response of regenerative tissues to plaque: a histological study in monkeys.
This study was designed to determine the histologic response of regenerated tissue to plaque, using an adult Taiwan monkey model with chronically-inflamed, surgically-created, periodontal defects. Standardized 2-walled periodontal defects were surgically created at the mesial of the mandibular lateral incisor on one side. Sutures, 3-0 braided silk, were placed for 8 weeks and tissues positioned so that denuded root surfaces were exposed to plaque, with the subsequent development of the chronically-inflamed periodontal tissues. At 8 weeks the defects were subjected to guided tissue regeneration procedures using expanded polytetrafluoroethylene membrane and allowed to heal for 6 months. Contralateral clinically healthy sites, without surgical procedures, were used as controls. In both experimental (membrane) and control sites, silk ligatures were placed to allow plaque accumulation for 2 or 10 weeks. Four monkeys were sacrificed at each time point and specimens processed for histologic and histometric study. The results indicated that plaque-induced inflammation was less at sites treated by guided tissue regenerative procedures when compared to originally clinically-healthy sites exposed to plaque for the same duration. However, histologically the newly formed osseous tissue was compromised under these conditions.
Ling
L J
LJ
Division of Periodontics, Veterans General Hospital-Taipei, School of Dentistry, National Yang Ming Medical College, Taiwan, R.O.C.
Lai
Y H
YH
Hwang
H
H
Chen
H
H
D016301:Alveolar Bone Loss / Q000473:pathology*; D000818:Animals; D001861:Bone Regeneration; D003094:Collagen / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism / Q000473:pathology; D003739:Dental Cementum / Q000502:physiology; D003773:Dental Plaque / Q000150:complications*; D004195:Disease Models, Animal; D016557:Guided Tissue Regeneration, Periodontal; D000882:Haplorhini; D008026:Ligation; D008297:Male; D008334:Mandible; D009504:Neutrophils; D015588:Observer Variation; D010010:Osteoclasts; D010513:Periodontal Ligament / Q000098:blood supply / Q000502:physiology; D010518:Periodontitis / Q000209:etiology / Q000473:pathology*; D015203:Reproducibility of Results
D003094:Collagen