Photoactivated gilvocarcin V induces DNA-protein crosslinking in genes for human ribosomal RNA and dihydrofolate reductase.
The nature of DNA interactions with photoactivated gilvocarcin V has been analyzed at the gene level in both rRNA and dihydrofolate reductase genes of human fibroblasts, utilizing a modified Southern hybridization technique. Neither interstrand DNA crosslinking nor RNA linkage to DNA was detected. However, we consistently observed in both genes retarded DNA bands appearing in a dose-dependent fashion following exposure to photoactivated gilvocarcin V. These retarded bands were enhanced when genomic DNA was prepared without proteinase K treatment, suggesting involvement of protein in this DNA interaction. Because these bands disappear following proteinase K treatment, it is probable that photoactivated gilvocarcin V induces DNA-protein crosslinking.
Matsumoto
A
A
Department of Radiation Biophysics and Genetics, Kobe University, School of Medicine, Japan.
Fujiwara
Y
Y
Elespuru
R K
RK
Hanawalt
P C
PC
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000528:radiation effects*; D015139:Blotting, Southern; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins; D004247:DNA / Q000187:drug effects*; D004275:DNA, Ribosomal / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000187:drug effects*; D005199:Fanconi Anemia; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005796:Genes / Q000187:drug effects*; D006027:Glycosides; D006801:Humans; D007364:Intercalating Agents / Q000494:pharmacology*; D008730:Methoxsalen / Q000494:pharmacology; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000494:pharmacology*; D012335:RNA, Ribosomal / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014983:Xeroderma Pigmentosum
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D003374:Coumarins; D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D006027:Glycosides; D007364:Intercalating Agents; D017319:Photosensitizing Agents; D012335:RNA, Ribosomal; C031781:gilvocarcin V; D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase; D008730:Methoxsalen