In vitro measurement of translation accuracy of ribosomes isolated from streptomycin-resistant mutant of Streptomyces granaticolor.
Accuracy of activity of ribosome isolated from UV-light-induced streptomycin-resistant R-21 mutant of Streptomyces granaticolor was measured in an E. coli-derived system translating poly(U) with a high rate and accuracy. Ribosomes from the R-21 mutant strain were shown to be resistant to streptomycin and about two-fold more accurate than those from the wild type. The mutant strain was found to be resistant to 1000 mg/L streptomycin (Stm) during vegetative growth while it sporulated on agar plates containing only up to 200 mg/L of Stm. The growth rate of the R-21 mutant in complex liquid medium was indistinguishable from that of the wild-type strain.
Institute of Microbiology, Academy of Sciences of Czech Republic, Prague.
J
J
Weiser
M
M
Ehrenberg
J
J
Náprstek
D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D009154:Mutation; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism*; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013307:Streptomycin / Q000494:pharmacology
D013307:Streptomycin