[Distribution of platelet membrane phospholipids in patients chronically hemodialyzed using cuprophane and cellulose acetate membranes].
The hemodialysis treatment is associated with a number of untoward events due to the blood-membrane contact. The severity of these reactions depends greatly on the kind of membrane. One of such effects is platelet activation with platelet membrane phospholipid translocation. The aim of this study was to assess the platelet membrane phospholipids distribution during hemodialysis through cuprophane (group I--12 patients) and cellulose acetate (group II--13 patients) membranes. Samples were taken before hemodialysis 14 days after treatment with the same dialyzer type. Phospholipids distribution was determined using a non penetrating tracer (TNBS) and by highly purified phospholipases hydrolysis. In patients of the group I a significant increase of phosphatidylserine and phosphatidylethanolamine at the outer platelet membrane surface was noted, as well as decrease in the other phospholipid classes, compared with control group. In patients of the group II the disturbances of phospholipid distribution were markedly smaller, and concerned only phosphatidylserine and sphingomyelin. Our data indicate that in patients dialyzed with the use of cuprophane membrane the disturbances of platelet membrane phospholipid distribution persist during the dialysis period. The milder intensity of these disturbances in patients treated using cellulose acetate suggests a better biocompatibility of this membrane.
Oddziału Chorób Wewnetrznych Szpitala Wojewódzkiego w Chełmie.
W
W
Krawczyk
K
K
Marczewski
A
A
Ledwozyw
A
A
Dmoszyńska
D000328:Adult; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives*; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D010743:Phospholipids / Q000378:metabolism*; D006435:Renal Dialysis / Q000295:instrumentation*
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D010743:Phospholipids; C005062:acetylcellulose; D002482:Cellulose; C009865:cuprammonium cellulose
Rozmieszczenie fosfolipidów błonowych krwinek płytkowych u pacjentów przewlekle hemodializowanych z uzyciem błon kuprofanowych i z octanu celulozy.