Characterization and localization of a putative oxytocin receptor in the cervix of the oestrous ewe.
The purpose of the present study was to investigate the presence of high-affinity oxytocin-binding sites (putative oxytocin receptors) in the cervix of the non-pregnant ewe. [3H]Oxytocin binding to the peripheral layers of cervical tissue (comprising the serosal layer and the least dense collagenous and muscular layers) and the remaining dense collagenous cervical tissue were studied separately. [3H]Oxytocin-binding sites were detected in membrane fractions prepared from both of these regions, but binding to the peripheral cervix was variable and binding site concentrations were low, hence these were not characterized further. A high-affinity oxytocin-binding site, having a dissociation constant of 1.8 nmol/l, was characterized in the dense collagenous regions of the cervix of ewes killed during the oestrous period. Similar dissociation constants were determined for [Arg8]-vasopressin and the oxytocin-specific agonist [Thr4, Gly7]-oxytocin in competition studies. [3H]Oxytocin binding to peripheral cervical tissue and to the dense collagenous cervix was generally low or undetectable during the luteal phase, but increased in both tissues around the time of luteolysis. Although specific binding to both tissues was variable during the oestrous period, it was higher at this time than during the luteal phase. [3H]Oxytocin-binding site concentrations were also found to be higher within the inner dense collagenous cervix of oestrous ewes than of pregnant, ovariectomized or anoestrous animals.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Anatomy, School of Medical Sciences, University of Bristol, UK.
E L
EL
Matthews
V J
VJ
Ayad
D000818:Animals; D001345:Autoradiography; D002584:Cervix Uteri / Q000737:chemistry*; D004971:Estrus / Q000097:blood / Q000378:metabolism*; D005260:Female; D011374:Progesterone / Q000097:blood; D011485:Protein Binding; D018045:Receptors, Oxytocin / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D012756:Sheep / Q000378:metabolism*
D018045:Receptors, Oxytocin; D011374:Progesterone