Lipase and acidic phosphatase from the psychrotrophic bacterium Pseudomonas fluorescens: two enzymes whose synthesis is regulated by the growth temperature.
In the psychrotrophic bacterium Pseudomonas fluorescens, the production of several enzymes that otherwise differ in their cell location, growth phase production and inducibility appeared to be similarly regulated by the growth temperature. In order to determine the level of this regulation, the expression of the apo and lip genes encoding two of these enzymes, the acidic phosphatase and lipase, respectively, was studied at different temperatures. Both genes were optimally expressed at 17.5 degrees C, i.e., at the optimal temperature of production of the enzymes; however, the low level of activity at the highest temperature could be due to an additional post-transcriptional control.
Laboratoire de Microbiologie du Froid, IUT de Rouen, Evreux, France.
J F
JF
Burini
B
B
Gügi
A
A
Merieau
J F
JF
Guespin-Michel
D000135:Acid Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D008049:Lipase / Q000096:biosynthesis* / Q000235:genetics; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D014169:Transformation, Bacterial; D001616:beta-Galactosidase / Q000378:metabolism
D012333:RNA, Messenger; D008049:Lipase; D000135:Acid Phosphatase; D001616:beta-Galactosidase