Electropherotypes and serotypes of human rotavirus in Estonia in 1989-1992.
The electropherotypes and serotypes of human rotaviruses circulating in Tallinn (Estonia) in 1989-1992 have been studied. Rotaviruses were found in 372 (25.8%) of 1,442 faecal specimens of pediatric patients with acute diarrhea. Polyacrylamide gel electrophoresis of RNA from 318 isolates has revealed 41 electropherotypes. Frequent genomic alterations, including a shift of predominant electropherotypes, were observed during the study period. The serotype of 158 (50.3%) of 314 tested rotavirus isolates was identified using ELISA with VP7-specific monoclonal antibodies against 4 established human rotavirus serotypes. Serotype G1 was found to be largely predominant and accounted for 70.9% of the typeable specimens, serotypes G4, G2, and G3 accounted for 12%, 9.5%, 7.6%, respectively. A major shift to serotype G4 took place in 1990-1991. Serotype G1 was represented by the largest number of electropherotypes. All G1, G3 and G4 isolates were of "long" and all G2 isolates were of "short" electropherotypes. According to our results the isolates of an identical electropherotype belong to the same serotype.
Ginevskaya
V A
VA
Institute of Poliomyelitis and Viral Encephalitides RAMS, Moscow, Russia.
Amitina
N N
NN
Eremeeva
T P
TP
Shirman
G A
GA
Priimägi
L S
LS
Drozdov
S G
SG
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D002213:Capsid / Q000032:analysis; D036022:Capsid Proteins; D002648:Child; D003967:Diarrhea / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004957:Estonia; D005243:Feces; D006801:Humans; D012330:RNA, Double-Stranded / Q000032:analysis / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification* / Q000302:isolation & purification*; D012621:Seasons; D012703:Serotyping; D017201:Virus Shedding
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; C050779:VP7 protein, Rotavirus