A one-step monoclonal antibody typing procedure that simplifies HLA class I and class II typing.
We have developed monoclonal antibodies to most HLA specificities, making it possible for us to devise a simple, rapid, one-step microcytotoxicity test. The test is performed by adding 1 microliter of cells to 1 microliter of antibody-complement mixture predotted on the microtest tray. The reactions are read following a 1-hour incubation period (30 minutes in some instances). The analysis of reactions seen on testing 105 class I antibodies and 50 class II antibodies is shown. A comparison of typing by the standard NIH method and the new one-step procedure showed a > 96% concordance in the 500 T cells and 200 B cells we examined. Class I and class II typing could be performed using B cells, thus obviating the need to isolate both T and B cells for HLA typing.
Lee
J H
JH
One Lambda, Inc., Canoga Park, CA.
Lias
M
M
Deng
C T
CT
Etessami
S
S
Loon
J
J
Chen
M
M
Banh
L
L
Conger
N
N
Connors
D
D
Manzo
A
A
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003080:Cold Temperature; D003165:Complement System Proteins / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D005260:Female; D006680:HLA Antigens / Q000032:analysis* / Q000145:classification / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000145:classification / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000145:classification / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008297:Male
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D003165:Complement System Proteins