Performance of two new enzyme immunoassays for the detection of IgM and IgG antibodies to rubella.
Two new enzyme immunoassays for detection of rubella-specific IgM and IgG antibodies, Rubaset EIA-M and Rubaset EIA-G, were evaluated. Serum samples from 350 patients with or without rubella symptoms were tested. Rubaset EIA-M had a sensitivity of 98.0%, a specificity of 95.2% and an overall agreement of 96.8% compared with Rubazyme-M. Sera from patients with autoimmune diseases showed no false-positive reactivity. The corresponding values for Rubaset EIA-G were 98.5%, 94.8% and 96.9% respectively, compared with Rubazyme. Sera yielding discordant results were mainly acute-phase specimens from patients with confirmed rubella infection.
Orion Corporation, Orion Diagnostica, Espoo, Finland.
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Granberg
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Meurman
D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D007223:Infant; D008297:Male; D015203:Reproducibility of Results; D012409:Rubella / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C040901:rubella antibodies