Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities.
Platelet-derived growth factor (PDGF) affects the growth, migration, and function in vitro of mesenchymal cells, but little is known about its normal physiological functions in vivo. We show here that mice deficient for PDGF B die perinatally and display several anatomical and histological abnormalities. Kidney glomerular tufts do not form, apparently because of absence of mesangial cells. Instead, a single or a few distended capillary loops fill the glomerular space. The heart and some large arteries dilate in late-stage embryos. Most PDGF B mutant embryos develop fatal hemorrhages just prior to birth. Their hematological status includes erythroblastosis, macrocytic anemia, and thrombocytopenia. On the basis of these findings, we conclude that PDGF B has crucial roles in vivo in establishing certain renal and circulatory functions.
Levéen
P
P
Department of Medical Biochemistry, University of Göteborg, Sweden.
Pekny
M
M
Gebre-Medhin
S
S
Swolin
B
B
Larsson
E
E
Betsholtz
C
C
D000740:Anemia / Q000235:genetics; D000818:Animals; D001158:Arteries / Q000002:abnormalities; D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics; D004899:Erythroblastosis, Fetal / Q000235:genetics; D005260:Female; D005313:Fetal Death / Q000235:genetics; D006330:Heart Defects, Congenital / Q000235:genetics*; D006402:Hematologic Diseases / Q000151:congenital / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D007668:Kidney / Q000002:abnormalities*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D010641:Phenotype; D010982:Platelet-Derived Growth Factor / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D011247:Pregnancy; D017384:Sequence Deletion; D013921:Thrombocytopenia / Q000235:genetics
D015342:DNA Probes; D010982:Platelet-Derived Growth Factor