Simulation and optimization of peptide separation by capillary electrophoresis.
The different mobility equations that have appeared in the literature for predicting peptide mobility were compared. A modified equation that relates the mobility of individual proteolytic species of a peptide to its composition has been obtained: mu = 1758 log(1 + 0.297q)/M0.411, where mu is the electrophoretic mobility in 10(-9) m2/s.V, q is the integral value of the charge of the species and M its molecular mass. Also, a rough estimation of the set of pKa values for a peptide was developed. The usefulness of this equation together with a computer program for predicting separations of compounds by capillary zone electrophoresis is demonstrated, employing real electropherograms of peptides from the literature or from experiments.
Laboratory for Analytical Chemistry, University of Amsterdam, Netherlands.
A
A
Cifuentes
H
H
Poppe
D000595:Amino Acid Sequence; D002021:Buffers; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification*
D002021:Buffers; D010455:Peptides