Overexpression of human thymidine kinase mRNA without corresponding enzymatic activity in patients with chronic lymphatic leukemia.
The level of cytosolic thymidine kinase (TK1) mRNA in lymphocytes from six healthy people and in lymphocytes from five patients with untreated chronic lymphatic leukemia (CLL) was determined with competitive polymerase chain reaction (competitive PCR). Using this procedure we have shown that in patients with CLL, there is an overexpression of TK1 mRNA without corresponding enzymatic activity. The TK1 mRNA level is approximately 100-fold higher in lymphocytes from CLL patients than in lymphocytes from healthy persons. A high level of TK1 mRNA without corresponding enzyme activity may indicate a defect in the processing of the enzyme. This may disturb the cells' normal feedback system and thereby influence the development of malignant conditions.
Roskilde University, Department of Life Sciences and Chemistry, Denmark.
T
T
Kristensen
H K
HK
Jensen
B
B
Munch-Petersen
D015152:Blotting, Northern; D003600:Cytosol / Q000201:enzymology; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000201:enzymology* / Q000235:genetics; D008214:Lymphocytes / Q000201:enzymology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism
D007527:Isoenzymes; D012333:RNA, Messenger; D013937:Thymidine Kinase