Establishment of HIV-1-producing cells from peripheral mononuclear cells cultured with normal human serum.
Normal human serum (NHS) contributed to the establishment of cells producing HIV-1 under the conditions of coculture of peripheral mononuclear cells (PMC) from HIV-1 seropositive patients and of PHA-prestimulated or -non-stimulated PMC from seronegative healthy donors. No addition of IL-2 and Polybrene was necessary. Since, in the case 90101, the mitochondrial displacement-loop DNA showed identical sequences in the established cells and the HIV-1 seropositive patient's cells, it can be asserted that the HIV-1-producing cells originated from the patient. These cells are still releasing HIV-1-virion more than one year after their establishment.
Kawashima
H
H
Department of Pediatrics, Tokyo Medical College, Japan.
Hoshika
A
A
Fukutake
K
K
Fujimaki
M
M
Mizuno
F
F
Iino
T
T
Tsukamoto
T
T
Tada
T
T
Nozaki-Renard
J
J
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D001483:Base Sequence; D001769:Blood; D002478:Cells, Cultured; D015497:HIV-1 / Q000254:growth & development*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D014776:Virus Cultivation
D015703:Antigens, CD