A new disaccharide Fuc alpha 1-2Man found in human urine.
Human urine collected from healthy individuals was ultrafiltered and the filtrate was gel-filtered. The fraction including disaccharides was pyridylaminated to convert the reducing sugars to fluorescent pyridylamino (PA)-derivatives. A PA-disaccharide consisting of Fuc and Man was purified by gel filtration, reversed-phase HPLC, and size fractionation HPLC. Structural analysis revealed that the disaccharide was Fuc alpha 1-2Man-PA. The disaccharide is considered to be a metabolite of unknown glycoconjugates.
Department of Chemistry, Osaka University College of Science.
J
J
Toyota
K
K
Omichi
S
S
Hase
D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D004187:Disaccharides / Q000652:urine*; D005374:Filtration; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data
D004187:Disaccharides; C090128:fucosyl-1-2-mannose