Identification of novel genes, SYT and SSX, involved in the t(X;18)(p11.2;q11.2) translocation found in human synovial sarcoma.
Human synovial sarcomas contain a recurrent and specific chromosomal translocation t(X;18)(p11.2;q11.2). By screening a synovial sarcoma cDNA library with a yeast artificial chromosome spanning the X chromosome breakpoint, we have identified a hybrid transcript that contains 5' sequences (designated SYT) mapping to chromosome 18 and 3' sequences (designated SSX) mapping to chromosome X. An SYT probe detected genomic rearrangements in 10/13 synovial sarcomas. Sequencing of cDNA clones shows that the normal SYT gene encodes a protein rich in glutamine, proline and glycine, and indicates that in synovial sarcoma rearrangement of the SYT gene results in the formation of an SYT-SSX fusion protein. Both SYT and SSX failed to exhibit significant homology to known gene sequences.
Department of Molecular Carcinogenesis, Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK.
J
J
Clark
P J
PJ
Rocques
A J
AJ
Crew
S
S
Gill
J
J
Shipley
A M
AM
Chan
B A
BA
Gusterson
C S
CS
Cooper
D000595:Amino Acid Sequence; D001483:Base Sequence; D002887:Chromosomes, Human, Pair 18; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D015321:Gene Rearrangement; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000235:genetics; D015183:Restriction Mapping; D013584:Sarcoma, Synovial / Q000235:genetics*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured; D014960:X Chromosome
D017931:DNA Primers; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm