31P NMR assessment of orthotopic liver rejection in a rat model.
31P NMR spectroscopy was used serially to study rejecting (ACl-->LEW) and nonrejecting (ACl-->ACl) orthotopic liver transplants in rats. Recipients were evaluated on post-transplant days 1, 3, 5, 7, 9, and 11. The relative changes in phosphomonoester (PME), inorganic phosphate (Pi), high-energy phosphates and pH were studied. The earliest significant difference between the rejecting and nonrejecting groups was a decrease in the alpha-NTP peak area on Day 5. This was followed by significant decreases in beta-NTP and pH, and increases in PME and Pi on Day 7. High-resolution 31P NMR spectra of perchloric acid extracts demonstrated the PME increase to be due mainly to elevated phosphoethanolamine. Using the parameter (Pi + PME)/(alpha + beta + gamma-NTP), rejecting livers were distinguished from nonrejecting livers at a moderate stage of rejection.
Department of Radiological Sciences, Deaconess Hospital, Boston, MA 02215.
J L
JL
Bowers
K
K
Kawano
K R
KR
Metz
K
K
Teramoto
A
A
McCullough
M E
ME
Clouse
D000818:Animals; D006084:Graft Rejection / Q000175:diagnosis* / Q000378:metabolism; D008099:Liver / Q000737:chemistry; D016031:Liver Transplantation; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010710:Phosphates / Q000032:analysis; D011685:Purine Nucleotides / Q000032:analysis; D051381:Rats; D011912:Rats, Inbred ACI; D011917:Rats, Inbred Lew; D012680:Sensitivity and Specificity
D010710:Phosphates; D011685:Purine Nucleotides