Synergistic spermicidal activity of neem seed extract, reetha saponins and quinine hydrochloride.
In order to identify potent spermicidal agents which are free from the side effects of currently available agents, spermicidal activity of purified neem seeds extract (Praneem), reetha saponins and quinine hydrochloride was studied individually and in combination. Sander-Cramer test was used to assess the activity on human sperm. Under the test conditions, minimum effective spermicidal concentrations for Praneem, reetha saponins and quinine hydrochloride were 25%, 0.05% and 0.346%, respectively. At these concentrations, 100% of the sperm were immobilised within 20 seconds. A positive synergistic effect in the spermicidal activity of these components, if used in combination, was observed which implies the use of reduced concentrations of each to bring about the desired action. The selected combination formulated into a suitable dosage form is likely to offer dual benefit of a potent contraceptive and an antimicrobial preparation.
Garg
S
S
National Institute of Immunology, JNU Complex, New Delhi, India.
Doncel
G
G
Chabra
S
S
Upadhyay
S N
SN
Talwar
G P
GP
D004338:Drug Combinations; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011803:Quinine / Q000008:administration & dosage / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects*; D013089:Spermatocidal Agents / Q000008:administration & dosage / Q000494:pharmacology*; D018709:Statistics, Nonparametric
D004338:Drug Combinations; D010936:Plant Extracts; C086286:Praneem; D012503:Saponins; D013089:Spermatocidal Agents; D011803:Quinine