Development of several organs that require inductive epithelial-mesenchymal interactions is impaired in LEF-1-deficient mice.
Lymphoid enhancer factor 1 (LEF-1) is a sequence-specific DNA-binding protein that is expressed in pre-B and T lymphocytes of adult mice, and in the neural crest, mesencephalon, tooth germs, whisker follicles, and other sites during embryogenesis. We have generated mice carrying a homozygous germ-line mutation in the LEF-1 gene that eliminates its protein expression and causes postnatal lethality. The mutant mice lack teeth, mammary glands, whiskers, and hair but show no obvious defects in lymphoid cell populations at birth. The LEF-1-deficient mice also lack the mesencephalic nucleus of the trigeminal nerve, the only neural crest-derived neuronal populations. Together, the pattern of these defects suggest an essential role for LEF-1 in the formation of several organs and structures that require inductive tissue interactions.
Howard Hughes Medical Institute, University of California, San Francisco 94143-0414.
C
C
van Genderen
R M
RM
Okamura
I
I
Fariñas
R G
RG
Quo
T G
TG
Parslow
L
L
Bruhn
R
R
Grosschedl
D000818:Animals; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D004627:Embryonic Induction; D005314:Embryonic and Fetal Development; D004848:Epithelium / Q000196:embryology*; D005804:Genes, Lethal; D018095:Germ-Line Mutation; D006197:Hair / Q000196:embryology; D051821:Lymphoid Enhancer-Binding Factor 1; D008321:Mammary Glands, Animal / Q000196:embryology; D008648:Mesoderm / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D009432:Neural Crest / Q000196:embryology; D014070:Tooth / Q000196:embryology; D014157:Transcription Factors / Q000032:analysis* / Q000235:genetics; D014276:Trigeminal Nerve / Q000196:embryology
D004268:DNA-Binding Proteins; C497574:Lef1 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1; D014157:Transcription Factors