Pig intestinal membrane-bound receptor (guanylyl cyclase) for heat-stable enterotoxin: cDNA cloning, functional expression, and characterization.
A cDNA encoding the receptor protein for a heat-stable enterotoxin (STa) produced by enterotoxigenic Escherichia coli was cloned from intestinal epithelial cells of a 10-week-old pig. The cDNA had an open reading frame of 3,219 base pairs and coded for a protein with 1,073 amino acid residues. The mature protein consisted of 1,050 amino acid residues with a molecular mass of ca. 121 kDa and was 87% and 82% identical with the human and rat protein, respectively. The CHO cell line overexpressing the pig recombinant STa receptor specifically bound to a photoaffinity-labeled analog of STa and showed marked elevation of the cellular content of cGMP in response to STa.
Institute for Protein Research, Osaka University, Japan.
A
A
Wada
T
T
Hirayama
S
S
Kitao
J
J
Fujisawa
Y
Y
Hidaka
Y
Y
Shimonishi
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism* / Q000633:toxicity; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D006152:Cyclic GMP / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004768:Enterotoxins / Q000378:metabolism* / Q000633:toxicity; D029968:Escherichia coli Proteins; D015870:Gene Expression; D006162:Guanylate Cyclase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry*; D008871:Microvilli / Q000737:chemistry; D008969:Molecular Sequence Data; D051381:Rats; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled; D018000:Receptors, Peptide / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014162:Transfection
D001427:Bacterial Toxins; D018076:DNA, Complementary; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D018000:Receptors, Peptide; D011994:Recombinant Proteins; C034151:heat stable toxin (E coli); D006162:Guanylate Cyclase; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled; D006152:Cyclic GMP