The pss4 gene from Rhizobium leguminosarum by viciae VF39: cloning, sequence and the possible role in polysaccharide production and nodule formation.
The Tn5 insertion into the genome of Rhizobium leguminosarum bv viciae VF39, resulting in non-mucoid growth and formation of non-N2-fixing nodule-like structures on Vicia faba plants, was mapped within a 1.4-kb EcoRV-SacI fragment. Nucleotide sequence analysis revealed an ORF (pss4) of 263 amino acids (aa). Three transcription start points (tsp) were determined. Two of them were localized upstream from the first GTG codon; the third tsp was mapped in front of the second putative start codon (GTG) corresponding to Val64 of the Pss4 aa sequence. The expression of pss4 in a T7 RNA polymerase/promoter system produced a single approx. 29-kDa protein. Pss4 reveals similarity to several proteins involved in polysaccharide biosynthesis in various Rhizobium species. A nearly complete homology was found with PssA from Rl biovar phaseoli 8002 [Borthakur et al., Mol. Gen. Genet. 213 (1988) 155-162], except that Pss4 has an additional 63 aa on its N terminus.
Ivashina
T V
TV
Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences, Pushchino, Moscow Region, Russia.
Khmelnitsky
M I
MI
Shlyapnikov
M G
MG
Kanapin
A A
AA
Ksenzenko
V N
VN
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial; D007887:Fabaceae / Q000382:microbiology; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010946:Plants, Medicinal; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D016961:Rhizobium leguminosarum / Q000235:genetics* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013559:Symbiosis; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C083492:EPSI polysaccharide; D008565:Membrane Proteins; D011135:Polysaccharides, Bacterial; C090524:Pss4 protein, Rhizobium leguminosarum; D012333:RNA, Messenger