Identification of FtsW and characterization of a new ftsW division mutant of Escherichia coli.
The product of the ftsW gene has been identified as a polypeptide that, like the related RodA protein, shows anomalous mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. FtsW is produced at low levels that can be increased by altering the translation initiation region of the mRNA. Overproduction of FtsW strongly inhibits cell growth. A new mutant allele, ftsW201, causes a temperature-dependent block in the initiation stage of cell division which is similar to the division block in ftsZ mutants. The block in initiation of division in the ftsW201 allele is shown to be independent of FtsZ or the FtsZ inhibitor, SulA. In addition, the ftsW201 mutant is hypersensitive to overproduction of the division initiation protein FtsZ at the permissive temperature. Our results suggest a role for FtsW in an early stage of division which may involve an interaction with FtsZ.
Institute of Cell and Molecular Biology, University of Edinburgh, Scotland.
M M
MM
Khattar
K J
KJ
Begg
W D
WD
Donachie
D000483:Alleles; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D003598:Cytoskeletal Proteins; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D008853:Microscopy; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D014176:Protein Biosynthesis; D017422:Sequence Analysis, DNA; D013489:Suppression, Genetic
D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D029968:Escherichia coli Proteins; C071525:FtsZ protein, Bacteria; D008565:Membrane Proteins; C053139:sulA protein, E coli; C061684:FtsW protein, Bacteria