Localization of leucokinin VIII in the cockroach, Leucophaea maderae, using an antiserum directed against an achetakinin-I analog.
An antiserum against an achetakinin analog selectively localized leucokinin VIII (LKVIII) in the CNS of Leucophaea maderae. Preabsorption studies of the achetakinin antiserum with either preimmune serum or LKVIII prevented a positive reaction in both ELISA and immunocytochemical procedures. LKVIII immunoreactive neurons were found in the brain, frontal, and subesophageal ganglion, all 3 thoracic ganglia and the terminal ganglion. Nerves originating from the thoracic and terminal abdominal ganglia contain LKVIII material. Lateral and medial neurosecretory cells synthesizing LKVIII-like products contribute axons to the nervi corporis cardiaci that terminate in neurohemal sites in the corpora cardiaca and nervi corporis allati. Thus, leucokinin VIII, like leucokinin I (LKI) and leucomyosuppressin (LMS), appears to have both a neurohemal and neurotransmitter mode of regulating target cells in L. maderae.
Food Animal Protection Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, College Station, Texas 77845.
S M
SM
Meola
F L
FL
Clottens
G M
GM
Coast
G M
GM
Holman
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002490:Central Nervous System / Q000737:chemistry*; D003058:Cockroaches / Q000737:chemistry*; D005260:Female; D017952:Ganglia, Invertebrate / Q000737:chemistry; D007106:Immune Sera; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000032:analysis*; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis*; D009842:Oligopeptides / Q000032:analysis*
D007106:Immune Sera; D007301:Insect Hormones; D009479:Neuropeptides; D009842:Oligopeptides; C079230:achetakinin