Rapid isolation of highly productive recombinant Chinese hamster ovary cell lines.
An expression system combining a unit for the expression of the gene of interest reinforced by the hepatitis B virus X transactivator and a selectable gene weakened by the insertion of an A+T-rich sequence derived from the 3'-untranslated region of the granulocyte-macrophage colony-stimulating factor mRNA is described. This vector allows rapid one-step isolation of highly productive Chinese hamster ovary clones.
Miloux
B
B
Sanofi Recherche, Centre de Labège, France.
Lupker
J H
JH
D016475:3T3 Cells; D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular / Q000379:methods; D006224:Cricetinae; D046508:Culture Techniques / Q000379:methods*; D005822:Genetic Vectors; D005914:Globins / Q000096:biosynthesis*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006367:HeLa Cells; D006511:Hepatitis B Antigens / Q000096:biosynthesis; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D007438:Introns; D007739:L Cells; D051379:Mice; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D015534:Trans-Activators / Q000096:biosynthesis; D014162:Transfection / Q000379:methods*; D054334:Viral Regulatory and Accessory Proteins
D006511:Hepatitis B Antigens; D007376:Interleukin-2; D012333:RNA, Messenger; D011994:Recombinant Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005914:Globins