Subcutaneous noradrenaline concentration increase in normal subjects during cold exposure evaluated by microdialysis.
Changes in local noradrenaline level in subcutaneous tissue were measured in four healthy males (23-33 years) during cold stimulation using a microdialysis technique. The subcutaneous dialysate noradrenaline concentration increased during cold exposure by a factor of 3.5 (P < 0.003). A correction factor due to plasma overflow of noradrenaline to subcutaneous tissue was calculated from noradrenaline infusion studies. This correction had a minimal effect on the observed increase in dialysate noradrenaline concentration and the level of significance was only slightly altered after correction for plasma overflow. The microdialysis technique in combination with a sensitive radioenzymatic noradrenaline assay may be useful to assess changes in local subcutaneous noradrenaline level in man during physiological stimulation.
Department of Internal Medicine and Endocrinology, Herlev Hospital, University of Copenhagen, Denmark.
B
B
Grønlund
L
L
Grønlund
N J
NJ
Christensen
D000328:Adult; D001794:Blood Pressure / Q000502:physiology; D003080:Cold Temperature; D006801:Humans; D008297:Male; D017551:Microdialysis; D009638:Norepinephrine / Q000097:blood* / Q000493:pharmacokinetics; D012881:Skin Temperature / Q000502:physiology
D009638:Norepinephrine