Effects of postnatal anti-nerve growth factor serum exposure on development of apical nerves of the rat molar.
The present study was undertaken to test the hypothesis that development of the pulpal innervation is dependent on nerve growth factor (NGF). Newborn rats were given subcutaneous injections of a rabbit anti-mouse NGF serum on alternate days for the first 24 days postnatally. Control animals were untreated and normal rabbit serum-treated litter mates. The animals were deeply anesthetized on postnatal day 26, perfused with fixative and the first mandibular molars were processed for transmission electron microscopy to obtain a complete census of axons entering the four roots. The composition of the mental nerve was also examined. Compared to control animals, the apical innervation of molars from anti-NGF-treated rats had only 62% as many myelinated fibers and 41% as many unmyelinated axons. Those myelinated fibers present in antiserum-treated animals were slightly, but significantly, smaller in average diameter than controls. In teeth of control animals, about 20% of all unmyelinated axons were located in fibers coursing outside of nerve fascicles; these isolated fibers were disproportionately rare after antiserum exposure. The average number of unmyelinated axons per Schwann cell unit was also significantly lower. Postnatal exposure to anti-NGF had milder effects on mental nerve composition compared to the tooth innervation. Numbers of myelinated fibers were 83% of controls, unmyelinated axons were 74% of controls and there was no change in the average number of unmyelinated axons per Schwann cell unit. We conclude that development of dental innervation is highly susceptible to postnatal NGF deprivation. This may be a consequence of the mostly nociceptive composition of dental nerves and their late development.
Department of Anatomy, University of Mississippi Medical Center, Jackson 39216.
J P
JP
Naftel
X B
XB
Qian
J M
JM
Bernanke
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D000906:Antibodies / Q000502:physiology*; D001369:Axons / Q000648:ultrastructure; D003782:Dental Pulp / Q000166:cytology / Q000254:growth & development*; D005260:Female; D008297:Male; D008854:Microscopy, Electron; D008963:Molar / Q000254:growth & development* / Q000294:innervation*; D009412:Nerve Fibers / Q000648:ultrastructure; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure; D009414:Nerve Growth Factors / Q000276:immunology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000906:Antibodies; D009414:Nerve Growth Factors