Expression of a novel Marek's disease virus type 1 (MDV1)-specific protein p40 in insect cells by a baculovirus vector and in chick embryo fibroblasts infected with MDV1.
We found that the open reading frame of 1053 nucleotides within the short unique sequence of Marek's disease virus (MDV) type 1 (MDV1) expresses a 40 kDa protein, using a baculovirus vector in insect cells. Mouse antisera against the 40 kDa protein reacted with a 40 kDa protein in MDV1-infected cells. A cross-reactive polypeptide of 37 kDa was also detected in cells infected with herpesvirus of turkey.
Chemo-Sero Therapeutic Research Institute, Kikuchi Research Center, Japan.
T
T
Urakawa
M
M
Sakaguchi
M
M
Yamamoto
G S
GS
Zhu
K
K
Hirai
D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D002460:Cell Line; D002642:Chick Embryo; D003429:Cross Reactions; D005347:Fibroblasts; D005814:Genes, Viral; D005822:Genetic Vectors; D008381:Herpesvirus 2, Gallid / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D018411:Spodoptera; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D014764:Viral Proteins