[Quantitative analysis of mRNA expression of the LDL receptor and HMG-CoA reductase in bovine tissue with competitive RT-PCR].
The present study exemplifies the method of quantitative reverse transcriptase-polymerase chain reaction (cRT-PCR) by quantifying, in bovine tissues, the specific mRNAs for the two key proteins which regulate cellular cholesterol metabolism, the low density lipoprotein (LDL)-receptor and the 3-hydroxy-3-methylglutaryl-Coenzyme A (HMG-CoA) reductase. Our data reveal a broad range of expression for both mRNAs. The LDL-receptor mRNA expression was highest in the adrenal gland (6.7 x 10(4) molecules/micrograms cellular RNA) followed by the corpus luteum of the ovary, intermediate in the liver (8.6 x 10(2) molecules/micrograms cellular RNA) and not detectable in the large intestine. Expression of the HMG-CoA reductase almost paralleled LDL-receptor values. It was found to be highest in the ovary (6.1 x 10(6) molecules/micrograms cellular RNA) followed by the adrenal gland, intermediate in the liver (3.1 x 10(5) molecules/micrograms cellular RNA) and lowest in the large intestine (9.9 x 10(4) molecules/micrograms cellular RNA). These data are consistent with other reports concerning the quantitative analysis of the expression of both proteins as determined by other experimental approaches. Thus cRT-PCR is a valuable tool for the quantitative analysis of the LDL-receptor and the HMG-CoA reductase.
Niendorf
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Institut für Pathologie, Universitäts-Krankenhaus Eppendorf, Hamburg.
Gebhardt
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D000311:Adrenal Glands / Q000473:pathology; D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000473:pathology; D005260:Female; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D007422:Intestines / Q000473:pathology; D008099:Liver / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics*; D011973:Receptors, LDL / Q000235:genetics*
D012333:RNA, Messenger; D011973:Receptors, LDL; D006903:Hydroxymethylglutaryl CoA Reductases
Quantitative Analyse der mRNA-Expression von LDL-Rezeptor und HMG-CoA Reduktase im bovinen Gewebe mittels kompetitiver RT-PCR.