Thermostability of bacterial luciferase expressed in different microbes.
Bacterial luciferase was used to investigate the relationship between the thermostability of a cytoplasmic reporter molecule and cellular heat resistance. The luciferase activity of Vibrio fischeri was expressed in strains of Escherichia coli, Salmonella typhimurium, Listeria monocytogenes and Brochothrix thermosphacta following transformation with plasmid pSP13 carrying the luxAB genes. The thermostability of intracellular luciferase varied depending on the organism in which it was expressed, but was not related to the cellular heat resistance of the different organisms. Addition of xylitol to the heating medium protected against loss of viability and inactivation of intracellular luciferase. Glycerol also protected against loss of viability but was less effective at preventing thermal denaturation of luciferase.
Institute of Food Research, Reading Laboratory, UK.
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Mackey
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Cross
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Park
D003470:Culture Media; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D005990:Glycerol; D006358:Hot Temperature; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism; D014733:Vibrio / Q000201:enzymology / Q000235:genetics; D014993:Xylitol
D003470:Culture Media; D008156:Luciferases; D005990:Glycerol; D014993:Xylitol