Localization of the gene (LAMA4) to chromosome 6q21 and isolation of a partial cDNA encoding a variant laminin A chain.
Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2. Variant chains such as merosin and S-laminin have been found in different tissues. We have isolated a cDNA encoding a novel laminin A variant that hybridizes to a 6.45-kb mRNA. Using amplification of genomic DNA and flow-sorted chromosomes we have assigned the gene (LAMA4) for this new laminin A variant to chromosome 6. Fluorescence in situ hybridization of a YAC clone further localized the gene to 6q21.
Dermatology Research Group, MRC Clinical Research Centre, Harrow, Middlesex, United Kingdom.
A J
AJ
Richards
L
L
al-Imara
N P
NP
Carter
J C
JC
Lloyd
M A
MA
Leversha
F M
FM
Pope
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D016384:Consensus Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D015394:Molecular Structure
D017931:DNA Primers; D018076:DNA, Complementary; D007797:Laminin; C084098:laminin A