Application of branched DNA signal amplification to monitor human immunodeficiency virus type 1 burden in human plasma.
A branched DNA (bDNA)-based quantitation of plasma human immunodeficiency virus type 1 (HIV-1) RNA was used to monitor the virologic status of 102 patients (29-906 CD4 cells/mm3) enrolled in clinical trials of antiretroviral and immune-based therapies. Virion-associated RNA was measurable in plasma of 74% of patients tested (10,000-10,000,000 RNA equivalents/mL). Virus levels measured by the bDNA assay exceeded titers obtained by quantitative plasma culture and were inversely correlated (r = -.378; P < .05) with total CD4 cell counts. The assay was used to demonstrate a significant decline (mean, 5-fold; range, 0- to 30-fold), relative to pretreatment, in virus load after beginning antiviral therapy and a transient increase (mean, 15-fold; range, 2- to 50-fold) after treatment with interleukin-2. The decrease in RNA was more dramatic than changes in serum p24 antigen. The bDNA assay yields reproducible results, is relatively easy, and should be useful in measuring HIV-1 RNA in patients in clinical trials.
Dewar
R L
RL
Department of Microbiology, Georgetown University Medical Center, Washington, DC.
Highbarger
H C
HC
Sarmiento
M D
MD
Todd
J A
JA
Vasudevachari
M B
MB
Davey
R T
RT
Kovacs
J A
JA
Salzman
N P
NP
Lane
H C
HC
Urdea
M S
MS
D000163:Acquired Immunodeficiency Syndrome / Q000821:virology*; D018791:CD4 Lymphocyte Count; D004279:DNA, Viral / Q000235:genetics*; D005821:Genetic Techniques; D016655:HIV Core Protein p24 / Q000097:blood; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D015203:Reproducibility of Results; D014766:Viremia / Q000821:virology*
D004279:DNA, Viral; D016655:HIV Core Protein p24; D012367:RNA, Viral