Method for the identification of saxitoxin in rat urine.
Saxitoxin (STX) is one of several related toxins that cause paralytic shellfish poisoning. We used solid-phase extraction (SPE) and prechromatographic oxidation/HPLC with fluorescence detection to isolate, identify, and quantify STX in rat urine. STX recovery from urine with the SPE procedure was approximately 76 +/- 6.5%. The standard curve was linear between 2 and 50 ng/ml. The lower limit of quantification with the method was 2 ng STX/ml of rat urine. Preliminary results with i.v. administration of STX to rats demonstrated that this method can detect and quantify STX in urine.
Toxinology Division, United States Army Medical Research Institute of Infectious Diseases, Ft. Detrick, Frederick, MD 21702-5011.
R G
RG
Stafford
H B
HB
Hines
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D008832:Microchemistry; D010084:Oxidation-Reduction; D051381:Rats; D012530:Saxitoxin / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine*
D012530:Saxitoxin