Contrary results on mouse hepatitis virus type 3 susceptibility in A/J mouse hepatocytes of phosphatidylserine treatment and of a hypercholesterolaemic diet: no correlation with membrane fluidity levels.
The aim of this study was to examine whether or not membrane fluidity directly influences infection by enveloped viruses, and, more precisely here, the susceptibility of A/J mouse hepatocytes to Mouse Hepatitis Virus type 3 (MHV3). We therefore studied, in parallel, the effects on hepatocyte membrane fluidity and on intracellular viral titre of two treatments, i) a hypercholesterolaemic diet to increase the hepatocyte membrane cholesterol content, ii) direct phosphatidylserine incorporation into hepatocyte membrane. Membrane fluidity was monitored on isolated hepatocytes by fluorescence anisotropy with TMA-DPH, and the viral titre was determined by plaque assay. The results clearly demonstrate that membrane fluidity is not directly involved in viral infection mechanisms.
Institut de Virologie de la Faculté de Médecine, Unité INSERM U74, Strasbourg, France.
H
H
Nonnenmacher
D
D
Illinger
J G
JG
Kuhry
A
A
Kirn
J
J
Braunwald
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D004198:Disease Susceptibility; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006937:Hypercholesterolemia / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000821:virology*; D008560:Membrane Fluidity; D051379:Mice; D008805:Mice, Inbred A; D006517:Murine hepatitis virus / Q000472:pathogenicity* / Q000502:physiology; D010718:Phosphatidylserines / Q000378:metabolism* / Q000494:pharmacology; D014779:Virus Replication
D002791:Cholesterol, Dietary; D005456:Fluorescent Dyes; D010718:Phosphatidylserines; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; D002784:Cholesterol