Transport stress delays the oestradiol-induced LH surge by a non-opioidergic mechanism in the early postpartum ewe.
The present study was designed to investigate whether transport, a mild environmental stressor, could affect the oestradiol-induced LH surge in postpartum ewes and, if so, the mechanism involved. Welsh Mountain ewes, with lambs removed at parturition (day 0) and hand-milked 12 and 48 h later, were given 50 micrograms oestradiol benzoate intramuscularly at various times postpartum. Blood samples were taken via an indwelling jugular venous catheter every 2 h from 8 to 24 h after oestradiol injection. All results are given as means +/- S.D. On day 1 oestradiol was unable to induce an LH surge in any ewe. Transport (10-14 h after oestradiol) delayed the onset of the oestradiol-induced LH surge on day 14 (17.5 +/- 1.7 vs 14.4 +/- 2.0 h, n = 5 each; P < 0.05), but not on day 28 (14.9 +/- 2.0 vs 14.0 +/- 2.4 h, n = 5 out of 7). Transport had no effect on the amplitude of the surge on either day. Naloxone treatment (1 mg/kg per 2 h) was unable to prevent the delay caused by transport (18.0 +/- 1.1 vs 17.5 +/- 1.7 h, n = 8 each), and did not affect the amplitude of the surge (28.4 +/- 5.3 vs 28.1 +/- 2.3 ng/ml, n = 8 each).(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Veterinary Clinical Science, University of Liverpool, Neston, South Wirral, UK.
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Smart
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Forhead
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Smith
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Dobson
D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D009270:Naloxone / Q000494:pharmacology; D049590:Postpartum Period / Q000378:metabolism*; D011247:Pregnancy; D012756:Sheep / Q000502:physiology*; D013312:Stress, Physiological / Q000378:metabolism*; D013997:Time Factors
D009270:Naloxone; D004958:Estradiol; D007986:Luteinizing Hormone