Stable strontium absorption as a measure of intestinal calcium absorption: comparison with the double-radiotracer calcium absorption test.
1. Stable strontium (Sr) has been proposed as an alternative to calcium (Ca) isotopes for the measurement of intestinal Ca absorption. The aim of this study was to compare the time course and fractional absorption of Ca and Sr, when both are measured using dual-tracer techniques. 2. 45Ca and Sr absorption tests were carried out on consecutive days in patients with osteoporosis (n = 10) or chronic renal failure (n = 7). Both tests were repeated in four patients with chronic renal failure after treatment with calcitriol (1 microgram daily for 10 days). 3. The time course of Ca absorption was determined using the 85Sr (intravenous)/45Ca (oral) dual-tracer technique, and the time course of Sr absorption using 85Sr (intravenous)/stable Sr (oral). Oral tracers were administered on consecutive days with a test meal containing 5.3 mmol of Ca and 2.5 mmol of either stable Sr or Ca carrier. The fractional absorption of 45Ca and Sr at 6h (FA360) and the absorption rate as a function of time were calculated by deconvolution. 4. The mean FA360 for Sr (20.2%) was lower than the mean FA360 for 45Ca (37.8%, P < 0.001, paired t-test), but the time course of Sr absorption was similar to that of Ca. There was a significant correlation between the FA360 for 45Ca and Sr, although the relationship was improved by including a quadratic term (R2 = 0.89, P < 0.001, significance of quadratic term, P < 0.05). After 1,25-dihydroxyvitamin D treatment, the FA360 of stable Sr increased 4.29-fold, whereas the FA360 of 45Ca increased only 2.4-fold.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Human Metabolism and Clinical Biochemistry, University of Sheffield, U.K.
A
A
Blumsohn
B
B
Morris
R
R
Eastell
D000328:Adult; D000368:Aged; D002117:Calcitriol / Q000627:therapeutic use; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D005260:Female; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology*; D007676:Kidney Failure, Chronic / Q000097:blood / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000097:blood / Q000378:metabolism; D013324:Strontium / Q000097:blood / Q000493:pharmacokinetics*; D013326:Strontium Radioisotopes; D013997:Time Factors
D002132:Calcium Radioisotopes; D013326:Strontium Radioisotopes; D002117:Calcitriol; D002118:Calcium; D013324:Strontium