Influence of cytostatic agents on the pulmonary defence of mice infected with Klebsiella pneumoniae and on the efficacy of treatment with ceftriaxone.
The effect of cytostatic treatment on the cellular defence and the efficacy of treatment with ceftriaxone in Klebsiella pneumoniae pneumonia was studied. Mice, made monocytopenic and granulocytopenic by cyclophosphamide or monocytopenic by etoposide, were infected intratracheally with K. pneumoniae (approximately 10(4) CFU) and then treated with ceftriaxone. At various intervals, the numbers of bacteria in the broncho-alveolar lavage (BAL) fluid and in lungs homogenised after lavage were determined. Cyclophosphamide reduced the numbers of granulocytes in the BAL fluid significantly but reduced only slightly the number of alveolar macrophages at the time of inoculation, 12 and 15 h later. The number of CFU in cyclophosphamide-treated mice was higher than that in controls, being significant in the homogenised lungs at 15 h after infection. In etoposide-treated mice, the numbers of alveolar phagocytes in BAL did not differ from those in control mice, whereas the number of bacteria was lower (only significantly in BAL fluid at 15 h after infection) than that in the controls. In this short experimental infection cytostatic treatment did not affect the outgrowth of Klebsiella pneumoniae substantially or the efficacy of treatment with ceftriaxone.
Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
W
W
Calame
A E
AE
Douwes-Idema
M T
MT
van den Barselaar
R
R
van Furth
H
H
Mattie
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002443:Ceftriaxone / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000494:pharmacology*; D004195:Disease Models, Animal; D005047:Etoposide / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007710:Klebsiella Infections / Q000276:immunology*; D007711:Klebsiella pneumoniae; D007970:Leukopenia / Q000139:chemically induced; D008171:Lung Diseases / Q000276:immunology*; D016676:Macrophages, Alveolar / Q000187:drug effects*; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*; D013047:Specific Pathogen-Free Organisms
D000970:Antineoplastic Agents; D007166:Immunosuppressive Agents; D005047:Etoposide; D002443:Ceftriaxone; D003520:Cyclophosphamide