[Spectrophotometric and chromatographic investigation of bilberry anthocyanins for qualification purposes].
Analysis of the bilberry anthocyanin extracts of different origin (French, Polish, Roumanian and Italian) was performed for qualification and identification. Direct spectrophotometry was considered as simple, informative method for controlling the purity of the extracts and proving the presence of polyphenols with the estimation of the ratio A1 cm1% 535/A1 cm1% 280. Thin-layer chromatography is an appropriate technique for the separation of 5 to 6 constituents in the extracts for qualification and for the evaluation of compound-ratios by TLC-densitometry. The new RP-HPLC gradient procedure seemed to be the best method for the standardization of bilberry extracts by the separation and quantitative estimation of 12 anthocyanins. Therefore it is of great value in the standardization of anthocyanin extracts and crude drugs containing them.
Semmelweis Orvostudományi Egyetem Gyógynövény- és Drogismereti Intézet, Budapest.
G
G
Petri
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U
Krawczyk
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A
Kéry
D000872:Anthocyanins / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D005602:France; D007558:Italy; D010946:Plants, Medicinal / Q000737:chemistry*; D011044:Poland; D012383:Romania; D013045:Species Specificity; D013055:Spectrophotometry, Infrared
D000872:Anthocyanins
A fekete áfonya termés antociánjainak spektrofotometriás és kromatográfiás vizsgálata minösítés céljából.