In vitro antiphagocytic effect of Melia azedarach leaf extracts on mouse peritoneal exudate cells.
The effect of Melia azedarach L. (Meliaceae) leaf extract on the phagocytic capability and respiratory burst of mouse peritoneal exudate cells was studied. The extract inhibited the phagocytosis of opsonized sheep erythrocytes. This inhibition was both dose- and time-dependent and reverted 48 h after removing the extract from the culture medium. Furthermore, chemiluminescence in treated cells was also impaired using either receptor (opsonized zymosan) or post-receptor (PMA) stimuli.
Laboratory of Virology, Faculty of Sciences, University of Buenos Aires, Argentina.
M C
MC
Courrèges
F
F
Benencia
C E
CE
Coto
E J
EJ
Massouh
F C
FC
Coulombié
D000818:Animals; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
D010936:Plant Extracts; D015054:Zymosan