Stabilization of the p53 gene product in two bovine leukemia virus infected cell lines.
Fetal lamb kidney cells (FLK) and bat lung (BAT2CL6) cells that continuously produce bovine leukemia virus (BLV) were found to cause malignant tumors in nude mice. Uninfected bat lung cells (Tb 1 Lu) produced a small benign neoplasm. Pulse chase studies showed that the p53 gene product in BAT2CL6 cells was more stable compared with p53 in Tb 1 Lu cells. Mono-clonal antibody studies suggested that a mutant form of the p53 protein was produced in BLV-infected cells. Heteroduplex mapping studies of the p53 gene from BLV-infected cells also suggested that a mutation in p53 had occurred. Stabilization of the p53 gene product in BLV-infected cells may contribute to the progression of tumor virulence.
Risk Analysis Section, Oak Ridge National Laboratory, TN 37831-6109.
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Dees
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Godfrey
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Foster
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Schultz
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Travis
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002685:Chiroptera; D002874:Chromosome Mapping; D004355:Drug Stability; D016583:Enzootic Bovine Leukosis / Q000382:microbiology; D016158:Genes, p53; D007668:Kidney / Q000166:cytology / Q000382:microbiology; D001909:Leukemia Virus, Bovine; D008168:Lung / Q000166:cytology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000473:pathology / Q000821:virology*; D009692:Nucleic Acid Heteroduplexes; D015531:Radioimmunoprecipitation Assay; D012756:Sheep; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
D009692:Nucleic Acid Heteroduplexes; D016159:Tumor Suppressor Protein p53