Tumor localization and therapeutic potential of an antitumor-anti-CD3 heteroconjugate antibody in human renal cell carcinoma xenograft models.
A heteroconjugate (HC) antibody, constructed with the monoclonal antibody (MoAB) Dal K29 to human renal cell carcinoma (RCC) and an anti-CD3 MoAb, could induce a very high level of lysis of human RCC cells when incubated with human peripheral blood lymphocytes (PBL) in vitro (Kerr et al., 1990, J. Immunol., 144, 4060-4067). We now report that this HC antibody selectively localizes in RCC xenografts in nude mice and could inhibit RCC in an ascites tumor xenograft model when administered intraperitoneally together with PBL.
Zhu
Z
Z
Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada.
Ghose
T
T
Lee
S H
SH
Fernandez
L A
LA
Kerr
L A
LA
Donohue
J H
JH
McKean
D J
DJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D000918:Antibody Specificity; D001345:Autoradiography; D017252:CD3 Complex / Q000276:immunology*; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000378:metabolism* / Q000628:therapy*; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive; D000922:Immunotoxins / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D007279:Injections, Subcutaneous; D007457:Iodine Radioisotopes; D007680:Kidney Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000628:therapy*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016853:Muromonab-CD3 / Q000493:pharmacokinetics / Q000627:therapeutic use; D009368:Neoplasm Transplantation; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D016853:Muromonab-CD3