Platelet plasminogen activator inhibitor 1 in patients with type II diabetes.
OBJECTIVE
To compare platelet plasminogen activator inhibitor 1 (PAI-1) concentration in type II diabetic patients and healthy control subjects.


METHODS
We studied a group of 12 diabetic patients whose disease was controlled by diet or sulfonylurea therapy and a group of 17 nondiabetic control subjects. All subjects were free of clinically advanced vascular disease. PAI-1 antigen concentrations were measured in 5 x 10(8) isolated platelets, which were lysed by 1% Triton X-100.


RESULTS
Mean platelet PAI-1 was significantly higher in diabetic patients (264 +/- 83 ng/5 x 10(8) platelets) compared with control subjects (202 +/- 71 ng/5 x 10(8) platelets) (P < 0.05). A significant independent positive correlation was found between platelet PAI-1 concentrations and fasting plasma specific insulin levels in the diabetic patients (r = 0.63, P = 0.03).


CONCLUSIONS
These findings suggest that 1) a higher platelet PAI-1 concentration may contribute to enhanced thrombosis in type II diabetes and 2) megakaryocyte PAI-1 synthesis may be under the control of insulin.
Ralph H. Johnson Department of Veterans Affairs Medical Center, Charleston, South Carolina 29401.
R
R
Jokl
M
M
Laimins
R L
RL
Klein
T J
TJ
Lyons
M F
MF
Lopes-Virella
J A
JA
Colwell
D001792:Blood Platelets / Q000378:metabolism*; D001794:Blood Pressure; D002096:C-Peptide / Q000097:blood; D002784:Cholesterol / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000503:physiopathology / Q000628:therapy; D003927:Diet, Diabetic; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood*; D010974:Platelet Aggregation; D010978:Platelet Factor 4 / Q000032:analysis; D012016:Reference Values; D014280:Triglycerides / Q000097:blood; D001620:beta-Thromboglobulin / Q000032:analysis
D002096:C-Peptide; D002784:Cholesterol; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D017395:Plasminogen Activator Inhibitor 1; D010978:Platelet Factor 4; D014280:Triglycerides; D001620:beta-Thromboglobulin