An Hsp60 related protein is associated with purified HIV and SIV.
The possible physical association of heat shock proteins (Hsp's) with immunodeficiency viruses (HIV and SIV) has been examined. The virions were purified by a) polyethylene glycol (PEG) precipitation and Sepharose 4B filtration, b) PEG precipitation and centrifugation over a Renografin gradient, or c) PEG precipitation and Matrex Cellufine Sulfate affinity chromatography. Western blotting revealed an Hsp60 related protein associated with HIV and SIV. Other Hsp's (such as Hsp70) were not detected, suggesting a specific interaction between Hsp60 and viral factors.
Dept. of Medical Microbiology and Immunology, Univ. of Wisconsin, Madison 53706.
S R
SR
Bartz
C D
CD
Pauza
J
J
Ivanyi
S
S
Jindal
W J
WJ
Welch
M
M
Malkovsky
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002460:Cell Line; D018834:Chaperonin 60 / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006678:HIV / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D011092:Polyethylene Glycols; D011485:Protein Binding; D015302:Simian Immunodeficiency Virus / Q000302:isolation & purification / Q000378:metabolism*; D013601:T-Lymphocytes; D014771:Virion / Q000302:isolation & purification / Q000378:metabolism
D000911:Antibodies, Monoclonal; D018834:Chaperonin 60; D011092:Polyethylene Glycols