1,25-Dihydroxyvitamin D3 effects in rat kidney: regulation of protein phosphorylation.
This study tested the hypothesis that 1,25-dihydroxyvitamin D3 treatment alters the pattern of protein phosphorylation in rat kidney. Treatment with 1,25-dihydroxyvitamin D3 (100 ng/day s.c. x 7d) in vitamin D-deficient rats markedly decreased phosphorylation of a particulate protein (91 +/- 0.6 kDa, n = 9) and moderately increased phosphorylation of a cytosolic protein (108 +/- 0.8 kDa, n = 9) in the kidney. The decreased phosphorylation of the 91-kDa particulate protein showed a graded dose response (0-200 ng/day), as did the more moderate increase in phosphorylation of the 108-kDa cytosolic protein. In conclusion, this study has provided evidence that 1,25-dihydroxyvitamin D3 plays an important role in the regulation of protein phosphorylation in rat kidney and adds these biochemical events to the growing list of 1,25-dihydroxyvitamin D3 effects in this poorly understood target tissue.
Department of Physiology, Tulane University School of Medicine, New Orleans, LA 70112.
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D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004988:Ethers, Cyclic / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014808:Vitamin D Deficiency / Q000378:metabolism
D004988:Ethers, Cyclic; D011506:Proteins; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases; D002117:Calcitriol