Effects of E5a and E7 genes of human papillomavirus type 11 on immortalized human epidermal keratinocytes and NIH 3T3 cells.
The E5a gene of HPV-11 expressed in NIH 3T3 cells led to tumorigenesis in nude mice; whereas when expressed in keratinocytes, E5a induced anchorage independent growth, but was nontumorigenic in nude mice. The E7 gene of HPV-11 expressed NIH 3T3 cells led to tumors in nude mice and morphological transformation, but not anchorage independent growth. Keratinocytes expressing the E7 gene induced colony formation in soft agarose, but not tumorigenesis in nude mice. Comparison of transforming activities of HPV-11 E5a and E7 genes of HPV-11 showed that the efficiency of cell transformation by E7 was weaker than that of E5a.
Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Y P
YP
Tsao
T Y
TY
Chu
T M
TM
Chen
Y F
YF
Yang
S L
SL
Chen
D016475:3T3 Cells; D000818:Animals; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D005814:Genes, Viral; D006801:Humans; D015603:Keratinocytes; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000209:etiology; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics* / Q000502:physiology; D013539:Simian virus 40 / Q000502:physiology
C081917:E5A protein, Human papillomavirus type 11; D009856:Oncogene Proteins, Viral