Isolation of human NuMA protein.
NuMA is a protein involved in maintenance of nuclear structure and in the assembly of the mitotic spindle. Expression of amino-terminal deletion mutants results in a phenotype identical to that caused by a temperature-sensitive defect of RCC1 (regulator of chromosome condensation). Here we describe the isolation of NuMA protein from HeLa cells under mild conditions as a prerequisite to study its interactions with elements of the RCC1-Ran regulatory pathway. In an overlay assay, NuMA did not bind Ran.[gamma-32P]GTP. Thus it is clearly different from Ran.GTP binding proteins of similar M(r).
Kempf
T
T
German Cancer Research Center, Division for Molecular Biology of Mitosis, Heidelberg.
Bischoff
F R
FR
Kalies
I
I
Ponstingl
H
H
D034961:Antigens, Nuclear; D018797:Cell Cycle Proteins; D002850:Chromatography, Gel; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins; D006160:Guanosine Triphosphate / Q000378:metabolism; D006367:HeLa Cells / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting; D007399:Interphase; D008938:Mitosis; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008941:Spindle Apparatus; D020931:ran GTP-Binding Protein
D034961:Antigens, Nuclear; D018797:Cell Cycle Proteins; C050045:NUMA1 protein, human; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins; D020931:ran GTP-Binding Protein