In vivo interaction of rabies virus phosphoprotein (P) and nucleoprotein (N): existence of two N-binding sites on P protein.
The rabies virus phosphoprotein (P) and nucleoprotein (N) are involved in transcription and replication of the viral genome. Interaction between N and P was studied in vivo in transfected cells expressing both proteins. Co-immunoprecipitation assays revealed that the N-P complex is present in cells expressing both proteins as well as in infected cells. Furthermore, immunostaining showed that coexpression of N and P was sufficient to induce the formation of cytoplasmic inclusions similar to those found in infected cells. In addition, deletion mutant analysis of P was performed to identify the regions of P interacting with N. The results indicate that at least two independent N-binding sites exist on P protein: one is located in the carboxy-terminal part of the protein and another between amino acids 69 and 177. The formation of cytoplasmic inclusions seems to require the presence of both N-binding sites on P protein.
Laboratoire de Génétique des Virus, CNRS, Gif sur Yvette, France.
M
M
Chenik
K
K
Chebli
Y
Y
Gaudin
D
D
Blondel
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D006224:Cricetinae; D017931:DNA Primers; D017353:Gene Deletion; D005814:Genes, Viral; D015151:Immunoblotting; D007668:Kidney; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D010750:Phosphoproteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011820:Rabies virus / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*
D017931:DNA Primers; D009698:Nucleoproteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014764:Viral Proteins