n-Butyrate enhances induction of thyroid hormone-responsive nuclear protein.
Effects of n-butyrate on nuclear thyroid hormone receptors and on thyroid hormone-responsive nuclear protein were investigated by means of a perfusion system in rat liver. Treatment with 5 mM n-butyrate resulted in an increase (150%) in the maximal binding capacity of 3,5,3'-L-triiodothyronine (T3) nuclear receptors without altering the affinity of receptor for T3. However, further perfusion for 4 h decreased the number of the receptors to the control level. n-Butyrate increased the amount of acetylated histone H4. The ability of nuclear T3 receptors to bind to core histones was diminished by acetylation of the core histones. Thyroid hormone-responsive nuclear protein (n protein) was increased by T3. The induction of the n protein by T3 was augmented by n-butyrate. These results suggested that n-butyrate modulates thyroid hormone-responsive gene expression in rat liver via the increased number of nuclear receptors or changes in the chromatin constitution.
Nishii
Y
Y
Department of Geriatrics, Endocrinology and Metabolism, Shinshu University School of Medicine, Matsumoto, Japan.
Ichikawa
K
K
Miyamoto
T
T
Takeda
T
T
Kobayashi
M
M
Suzuki
S
S
Sakurai
A
A
Hashizume
K
K
D000107:Acetylation; D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002467:Cell Nucleus / Q000737:chemistry*; D002843:Chromatin / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006657:Histones / Q000096:biosynthesis / Q000235:genetics; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D010477:Perfusion; D011499:Protein Processing, Post-Translational; D051381:Rats; D017208:Rats, Wistar; D011988:Receptors, Thyroid Hormone / Q000096:biosynthesis* / Q000187:drug effects; D014284:Triiodothyronine / Q000378:metabolism*; D015854:Up-Regulation
D002087:Butyrates; D002843:Chromatin; D006657:Histones; D009687:Nuclear Proteins; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D020148:Butyric Acid