Expression of tumor-associated glycoantigen, sialyl Lewis(a), in human head and neck squamous cell carcinoma and its application to tumor immunotherapy.
The glycoantigen sialyl Lewis(a) (sLe(a)) is widely expressed on a variety of gastrointestinal tumor cells. Here, we immunohistochemically demonstrated the expression of sLe(a) antigen in 54% (7 out of 13) of human head and neck squamous cell carcinoma (H-NSCC) samples. Frequent expression of sLe(a antigen was also demonstrated on a variety of H-NSCC cell lines using flow cytometry. Both CD4+ and CD8+ T cells, which were activated with immobilized OKT3 monoclonal antibody plus interleukin-2, showed augmented cytotoxicity against sLe(a)-positive H-NSCC, including autologous tumor cells, on targeting with anti-CD3 x anti-sLe(a) bispecific antibody, suggesting that sLe(a) antigen is a good target molecule for bispecific antibody-dependent adoptive tumor immunotherapy of human head and neck cancer.
Department of Otorhinolaryngology, Tokai University School of Medicine, Isehara.
K
K
Makino
T
T
Ogata
H
H
Miyake
S
S
Habu
T
T
Nishimura
D000328:Adult; D000368:Aged; D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D018395:CA-19-9 Antigen; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000628:therapy; D003602:Cytotoxicity, Immunologic; D005260:Female; D005732:Gangliosides / Q000276:immunology*; D006258:Head and Neck Neoplasms / Q000276:immunology* / Q000628:therapy; D006801:Humans; D007111:Immunity, Cellular; D007167:Immunotherapy; D066298:In Vitro Techniques; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured
D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D018395:CA-19-9 Antigen; D005732:Gangliosides; D007983:Lewis Blood Group Antigens; C064564:sialyl Le(a) ganglioside