Characterization of cytochrome P450-monooxygenases of Chinese hamsters with respect to aflatoxin B1 activation.
Monooxygenases of Chinese hamster livers were characterized with respect to aflatoxin B1 activation and cytochrome P450 isozymes. Polychlorinated biphenyl (PCB)-treatment induced higher activity for aflatoxin B1 activation in Chinese hamsters than in rats and this activity was more elevated by the treatment with phenobarbital than with 3-methylcholanthrene, which is contrary to the observation in Syrian hamsters and rather similar to that in rats. Western blot analysis demonstrated that the treatment of Chinese hamsters with phenobarbital markedly elevated the level of cytochrome P450 isozyme belonging to the CYP2A family and only slightly that immuno-related to CYP2B1. In parallel with this, the activities of testosterone 15 alpha-hydroxylase and 7-ethoxycoumarin O-deethylase were significantly induced by phenobarbital treatment. These suggest that phenobarbital mainly induces an isozyme related to CYP2A2 which is responsible for aflatoxin B1 activation in Chinese hamsters.
Department of Pharmaceutical Sciences, National Institute of Public Health, Tokyo, Japan.
M
M
Fukuhara
E
E
Antignac
N
N
Fukusen
K
K
Kato
M
M
Kimura
D016604:Aflatoxin B1 / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D006224:Cricetinae; D003412:Cricetulus / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005260:Female; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D010634:Phenobarbital / Q000494:pharmacology; D013045:Species Specificity
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System; D016604:Aflatoxin B1; D010634:Phenobarbital