Instant RNA isolation from virus-infected tissue culture fluid for the polymerase chain reaction.
This paper describes an efficient method of RNA isolation, enabling RNA templates to be obtained for reverse transcription within 20 min. The procedure avoids the use of hazardous organic chemicals and ethanol precipitation, and does not require ultracentrifugation. Results are presented for extraction of RNA from virus-infected tissue culture fluid, infected cell sheets, vaccine bulk material and lyophilized commercial vaccines.
Division of Virology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts, UK.
M A
MA
Afzal
P D
PD
Minor
D002478:Cells, Cultured; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000302:isolation & purification*
D012367:RNA, Viral