Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum.
The complementary DNA (PsU BC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys85) found in UBCs so far described is also conserved in the P. sativum sequence.
Department of Plant Pathology, University of Arizona, Tucson 85721.
H H
HH
Woo
L A
LA
Brigham
M C
MC
Hawes
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D044763:Ubiquitin-Conjugating Enzymes
D018076:DNA, Complementary; D012333:RNA, Messenger; D044763:Ubiquitin-Conjugating Enzymes; C063109:ubiquitin-conjugating enzyme UBC4; D008025:Ligases