[Preliminary study on expression of Leishmania donovani antigen gene in E. coli].
We constructed previously the expressing library of Leishmania donovani genomic DNA with lambda gt11 as vector. In this paper, 2 x 10(4) phages were plated on E. coli Y1090r-, and screened with a rabbit antiserum prepared by immunization with Leishmania donovani promastigotes. Bound antibodies were detected using alkaline phosphatase labeled anti-rabbit antibodies. A positive expressing clone was detected and transferred into E. coli Y1098r- to prepare lysate, a 39 kDa polypeptide in E. coli Y1089r-lysate was recognized by anti-Leishmania donovani serum. The result indicated that the 39 kDa polypeptide which was not fused with the major portion of beta-galactosidase existed disconnectedly. This finding remained to be further studied.
Jing
B
B
Laboratory of Parasitology, West China University of Medical Sciences, Chengdu.
Hu
X
X
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D017125:Genes, Protozoan; D015698:Genomic Library; D007893:Leishmania donovani / Q000235:genetics*; D010452:Peptide Biosynthesis
D000953:Antigens, Protozoan