Role of H-ras in the malignant progression of rat tracheal epithelial cells.
The effect of an activated H-ras oncogene on the progression of neoplasia was studied in transformed rat tracheal epithelial cells. Nude mouse tumours produced by injection of these cells exhibited a higher fraction of cells containing the mutant ras gene that did the injected cells, while a subclone that lacked the ras mutation was much less tumorigenic than parental cells. Serial passage of one cell line containing a ras mutation resulted in an increase in the fraction of ras-mutated cells, which suggests that, in this line, ras activation may confer a selective advantage in vitro as well. However, this was not seen in another ras-containing line, suggesting the importance of alternative pathways in malignant progression of rat tracheal epithelial cells.
Institute of Environmental Medicine, New York University Medical Center, New York 10016.
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G
Cosma
F
F
Hubbard
R J
RJ
Jamasbi
A
A
Marchok
S J
SJ
Garte
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004848:Epithelium / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009154:Mutation; D051381:Rats; D011916:Rats, Inbred F344; D014132:Trachea / Q000502:physiology*; D014134:Tracheal Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured