Mapping of the RNA-binding domain of the alfalfa mosaic virus movement protein.
In-frame contiguous deletions were created in the movement protein gene of alfalfa mosaic virus by site-directed mutagenesis. The mutated movement proteins were expressed in Escherichia coli, extracted and then purified by denaturing gel electrophoresis and then renatured. Their binding ability with RNA was assayed by electrophoretic retardation and u.v.-crosslinking. Results indicated that a domain included within amino acids 36 to 81 was necessary for RNA binding.
Institut de Biologie Moléculaire des Plantes, Université Louis Pasteur, Strasbourg, France.
F
F
Schoumacher
C
C
Giovane
M
M
Maira
A
A
Poirson
T
T
Godefroy-Colburn
A
A
Berna
D017794:Alfalfa mosaic virus / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames; D053830:Plant Viral Movement Proteins; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D017384:Sequence Deletion; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*
D053830:Plant Viral Movement Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins