Effect of pyridine and its methylated derivatives on storage by rat pineal nerves of monoaminergic neurotransmitter.
In previous work of our laboratory, it was demonstrated that collidine (2-4-6-trimethylpyridine) applied briefly to fresh tissues extracted noradrenaline or closely related compound/s of neuronal origin. This effect gave rise to the abolition of osmiophilia and chromaffin reaction of electron-dense cores of monoaminergic synaptic vesicles and to the extraction of radioactive compounds in tissues that had previously taken up tritiated noradrenaline. In this work, the role of the pyridine ring and its progressive methylation on the monoamine releasing effect was investigated. For this purpose, the effect of pyridine, two picolines (2 and 4 monomethylpyridines) and a lutidine (2-6-dimethylpyridine) was compared to the effect of collidine. It was found that pyridine has a much smaller effect than collidine on the histochemical reactivity of monoaminergic synaptic vesicles and on the extraction of tritiated compounds and that its extent was dependent upon the number of methyl groups incorporated in the pyridine ring.
Instituto de Biología Celular, Facultad de Medicina, Universidad de Buenos Aires, República Argentina.
A
A
Pellegrino de Iraldi
J P
JP
Corazza
R
R
Tomás
D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D002838:Chromaffin System / Q000378:metabolism; D006651:Histocytochemistry; D008297:Male; D008745:Methylation; D009992:Osmium; D010870:Pineal Gland / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D013572:Synaptic Vesicles / Q000378:metabolism; D016707:Tissue Fixation
D015306:Biogenic Monoamines; D011725:Pyridines; D009992:Osmium; C007106:gamma-collidine