Methods for the analysis of the new vinca alkaloid derivative, S 12363, in plasma by high-performance liquid chromatography with fluorescence detection.
Two sensitive analytical methods for the analysis of S 12363 in plasma are described. A highly sensitive procedure for human and dog plasma using cyanopropyl solid-phase extraction with ion pairing chromatography and fluorescence detection, has a limit of quantification of 0.1 ng ml-1. The technique has an overall precision and accuracy of 4.8 and 5.4% respectively over the concentration range 0.1-20 ng ml-1. A second, less sensitive, assay specifically adapted for rodent plasma, uses benzene sulphonyl cation-exchange solid-phase extraction followed by reversed-phase chromatography, with post-column fluorescence enhancement. This method has a limit of quantitation of 1.0 ng ml-1, with overall accuracy and precision of 7.2 and 11.6% respectively, over the concentration range 1.0-20.0 ng ml-1. Both assays have been successfully applied to dog and mouse toxicokinetic studies.
Servier Research and Development, Fulmer, Slough, UK.
B H
BH
Gordon
D M
DM
Bakes
P
P
Bennett
B
B
LeBras
B
B
Walther
C
C
Lucas
S
S
Marc
A
A
Gouyette
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood*; D001555:Benzene Derivatives; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004285:Dogs; D006536:Heptanes; D006801:Humans; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D051379:Mice; D008832:Microchemistry; D015394:Molecular Structure; D011786:Quality Control; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D014748:Vinca Alkaloids / Q000097:blood* / Q000493:pharmacokinetics / Q000633:toxicity
D000972:Antineoplastic Agents, Phytogenic; D001555:Benzene Derivatives; D006536:Heptanes; D007202:Indicators and Reagents; D014748:Vinca Alkaloids; C064005:S 12363