The tetratricopeptide repeat-domain of the PAS10 protein of Saccharomyces cerevisiae is essential for binding the peroxisomal targeting signal-SKL.
The PAS10 gene was found in a two-hybrid screen for the isolation of genes encoding proteins which interact with the C-terminal peroxisomal targeting signal -SKL. The PAS10 protein is known to be involved in import of proteins into peroxisomes and to contain a tetratricopeptide repeat (TPR) domain. All TPR-containing proteins involved in diverse processes like mitosis or RNA-synthesis share the ability to interact with other proteins. Here we show that the PAS10 protein interacts in vivo with the C-terminal peroxisomal targeting signal. The part essential for this interaction contains the complete tetratricopeptide repeat domain.
Brocard
C
C
Institut für Biochemie und Molekulare Zellbiologie der Universität Wien, Austria.
Kragler
F
F
Simon
M M
MM
Schuster
T
T
Hartig
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000378:metabolism*; D005656:Fungal Proteins / Q000096:biosynthesis / Q000378:metabolism*; D005800:Genes, Fungal; D026901:Membrane Transport Proteins; D008830:Microbodies / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D000074435:Peroxisome-Targeting Signal 1 Receptor; D010957:Plasmids; D021382:Protein Sorting Signals / Q000737:chemistry / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
D002352:Carrier Proteins; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; C499509:PEX5 protein, S cerevisiae; D000074435:Peroxisome-Targeting Signal 1 Receptor; D021382:Protein Sorting Signals; D029701:Saccharomyces cerevisiae Proteins