Respiratory epithelial permeability in complete Freund's adjuvant rat lung granulomatosis.
To investigate Freund's adjuvant-induced changes in respiratory epithelial permeability, we determined the respiratory epithelium clearance of 99mTc-DTPA (RC-DTPA) in rats and the localization of horseradish peroxidase (HRP) across the lung epithelium. RC-DTPA was determined before and after intravenous injection of saline (control) or complete Freund's adjuvant (CFA). Although the respiratory epithelium was not disrupted, RC-DTPA was increased 15 min after injection of CFA and was still high after 3 d but had returned to normal after 7 d, after which it again increased and stayed high from Day 42 through Day 112. Cell number obtained by bronchoalveolar lavage increased from 15 min to 3 d after injection of CFA. Lung granulomatosis was observed 14 to 112 d after injection of CFA; changes in RC-DTPA correlated with changes in alveolar cells. HRP was present in bronchiolar intercellular spaces only in the CFA group. We conclude that (1) RC-DTPA increases in rat lung CFA granulomatosis 15 min to 3 and 42 to 112 d following injection, (2) the increase in RC-DTPA is not related to a rupture of epithelium but (3) is proportional to the cell influx into the lung, and (4) is possibly caused by the opening of tight junctions in small airways.
Université de Paris René Descartes, Laboratoire de Biologie et Pharmacologie des Epitheliums Respiratoires, France.
M
M
Mordelet-Dambrine
G
G
Stanislas-Leguern
D
D
Henzel
L
L
Barritault
G
G
Huchon
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004195:Disease Models, Animal; D004848:Epithelium / Q000000981:diagnostic imaging / Q000187:drug effects / Q000378:metabolism; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006099:Granuloma / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D008168:Lung / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism; D008171:Lung Diseases / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000378:metabolism*; D008297:Male; D010544:Peroxidases / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D016284:Technetium Tc 99m Pentetate; D013997:Time Factors
D005620:Freund's Adjuvant; D006735:Horseradish Peroxidase; D010544:Peroxidases; C021799:microperoxidase; D016284:Technetium Tc 99m Pentetate