Colorimetric micromethod for protein determination with erythrosin B.
Reaction of erythrosin B with proteins results in a stable, highly colored chromophore with an absorbance maximum at 545 nm. This is the basis for a new quantitative determination method of proteins in solution. The assay can be performed at room temperature, but is faster and more sensitive at 90-95 degrees C. The erythrosin assay is characterized by (i) stable dye-protein color, (ii) high sensitivity (2-14 micrograms/ml protein), (iii) short reaction time (1.5-2 min at 90-95 degrees C), (iv) good reproducibility, (v) limited interference by common reagents, and (vi) low protein-to-protein variability. Thus, the erythrosin assay can be useful for routine analytical purposes and may overcome some of the limitations of other currently employed assays.
Kraft General Foods, Tarrytown, New York 10591.
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Soedjak
D016477:Artifacts; D003124:Colorimetry / Q000191:economics / Q000379:methods*; D004923:Erythrosine; D006863:Hydrogen-Ion Concentration; D008846:Micromanipulation / Q000191:economics; D015394:Molecular Structure; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
D011506:Proteins; D004923:Erythrosine