[Anticancer effect of balloon laserthermia on human cholangiocellular carcinoma].
The anticancer effect of the Balloon Laserthermia on human cholangiocellular carcinoma, HuCC-T1, producing the tumor marker CA19-9, was examined in order to investigate the possibility of applying the Balloon Laserthermia to patients with advanced bile duct cancer. A Simple hyperthermia (STt; t:min) or the Balloon Laserthermia (TBLTt; T:temperature, t:min) was performed on the cultured HuCC-T1 cells 96 hours after plating 1 x 10(4) cells in each well. The author observed the cells, photographed them through the microscope, and collected every supernatants for measuring CA19-9. Next, the author calculated the number of living HuCC-T1 cells 24 and 48 hours after each treatment. After lysating living HuCC-T1 cells (1 x 10(4)), CA19-9 was measured in Control, ST15, 42BLT15 and 37BLT15 groups. In in vivo experiments, the HuCC-T1 cells (1 x 10(7)) were subcutaneously inoculated into nude mice, and the mice were then treated with ST15, 42BLT15 or 42BLT30 14 days after the inoculation. Each tumor was resected and measured 7 days after the treatment, respectively, and the specimen of the tumor was microscopically examined. The anticancer effect of ST15 on HuCC-T1 cells appeared at the 48 hour point, showing a hyperthermic effect at a delayed stage. The anticancer effect of BLT appeared at the 24 hour point, showing cytocidal effect at an early stage, and this effect continued, being accompanied by hyperthermia. The 42BLT15 represented the highest anticancer effect of all experimental groups. The values of CA19-9 in the supernatants did not show a significant difference between any two groups at their respective harvest times. The values in the living HuCC-T1 cells treated with BLT significantly decreased, compared with the Control and ST15 groups. The tumor wet weight was significantly lower in 42BLT groups, compared with the Control or ST15 groups. The degeneration and/or destruction of the in vitro HuCC-T1 cells and the in vivo tissue-necrosis were very prominent in the BLT groups. The Balloon Laserthermia clearly brought about both cytocidal effect at an early stage and hyperthermic effect at a delayed stage after the treatment. The Laserthermia also presented the possibility of a qualitative degeneration of the cancer cells. The Balloon Laserthermia was considered to be useful for clinical applying.
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
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Koito
D000818:Animals; D001650:Bile Duct Neoplasms / Q000473:pathology* / Q000628:therapy; D002404:Catheterization; D018281:Cholangiocarcinoma / Q000473:pathology* / Q000628:therapy; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D017075:Laser Coagulation; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured