Simultaneous high-performance liquid chromatographic determination of residual sulphamonomethoxine, sulphadimethoxine and their N4-acetyl metabolites in foods of animal origin.
A rapid and sensitive method for the determination of residual sulphamonomethoxine, sulphadimethoxine and their N4-acetyl metabolites in beef, pork, chicken and eggs by high-performance liquid chromatography (HPLC) was developed. The extraction of these compounds was performed using a mixture of 90% (v/v) acetonitrile solution and hexane (5:4, v/v) to minimize the fat content followed by purification by alumina column chromatography. These extracts contained sulphonamide analytes which were free from interfering compounds when examined by HPLC using a LiChrosorb RP-18 column. The average recoveries from spiked meat and egg were in excess of 80% with relative standard deviations between 0.4 and 5.0%. The practical limits of detection were 0.01 ppm for all samples.
School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Japan.
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N
Furusawa
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T
Mukai
D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs / Q000032:analysis; D005504:Food Analysis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis; D013412:Sulfadimethoxine / Q000031:analogs & derivatives / Q000032:analysis*; D013422:Sulfamonomethoxine / Q000031:analogs & derivatives / Q000032:analysis*; D013552:Swine
D007202:Indicators and Reagents; C038180:N(4)-acetylsulfadimethoxine; D013412:Sulfadimethoxine; C051785:N(4)-acetylsulfamonomethoxine; D013422:Sulfamonomethoxine