Viral genetic determinants in SIVsmmPBj pathogenesis.
A variant simian immunodeficiency virus (SIV) from sooty mangabeys, SIVsmmPBj, induces an acutely lethal disease in pigtailed macaques (Macaca nemestrina). This study further characterizes the viral genetic determinants involved in this acutely lethal disease. We have generated chimeric molecular clones constructed between SIVsmmPBj and either SIVsmH4 or SIVsmm9 to analyze the role of the 5' half of the genome and the envelope gene in the induction of acute disease. These studies suggest that the gag and gp40 of SIVsmmPBj are required for the development of lethal disease, and an additional determinant in the central regulatory gene region of the SIVsmmPBj genome is also required.
Division of Pathobiology and Immunobiology, Yerkes Regional Primate Research Center, Emory University, Atlanta, GA.
F J
FJ
Novembre
M M
MM
Saucier
V M
VM
Hirsch
P R
PR
Johnson
H M
HM
McClure
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002678:Chimera; D003001:Cloning, Molecular; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D015683:Gene Products, gag / Q000096:biosynthesis / Q000235:genetics; D015750:Genes, gag; D016679:Genome, Viral; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000276:immunology / Q000821:virology; D008254:Macaca nemestrina; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D016097:Simian Acquired Immunodeficiency Syndrome / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
D017931:DNA Primers; D004279:DNA, Viral; D015683:Gene Products, gag