Multiple receptor-like protein tyrosine phosphatases in the form of chondroitin sulfate proteoglycan.
The possibility that some of the brain proteoglycans are receptor-like protein tyrosine phosphatases (PTPases) was investigated. Membrane-bound proteoglycan fractions were prepared from the postnuclear membrane fraction of 8-day-old rat brain by DEAE ion-exchange chromatography and CsCl density gradient centrifugation. The isolated proteoglycan fractions showed high PTPase specific activities together with the typical PTPase characteristics. Renaturation experiments indicated that chondroitin sulfate proteoglycans with 380- and 170-kDa core proteins carried the PTPase activity. The proteoglycan with 380-kDa core protein was identified as RPTP beta/zeta bearing HNK-1 carbohydrate.
Division of Molecular Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
N
N
Maeda
H
H
Hamanaka
T
T
Shintani
T
T
Nishiwaki
M
M
Noda
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D003001:Cloning, Molecular; D015723:Gene Library; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology
D011508:Chondroitin Sulfate Proteoglycans; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D017027:Protein Tyrosine Phosphatases; C516897:Ptprz1 protein, rat; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5