Interaction of enteropathogenic Yersinia enterocolitica with complex basement membranes and the extracellular matrix proteins collagen type IV, laminin-1 and -2, and nidogen/entactin.
The plasmid-encoded virulence factor yersinia adhesin A (YadA) contributes to pathogenicity of Yersinia enterocolitica which might be related to its adhesive potential. Therefore, we have investigated the interaction of Y. enterocolitica with basement membrane (BM) and with the major BM proteins collagen type IV, laminin, and nidogen/entactin. Recombinant YadA-positive but not YadA-negative yersiniae bound specifically to lens capsule BM tissue, as well as purified collagen type IV and the laminin-1 and -2 (formally known as merosin) isoforms. Binding sites are located on the alpha 1 chain of the 58-nm amino-terminal 7sL fragment of collagen type IV and on the elastase-fragment E1 of laminin-1. YadA-mediated binding of yersiniae to collagen type IV was rapid and saturable, it was independent of divalent cations, stable over a wide pH range, and not influenced by higher salt concentrations. D-Glucose and D-galactose did not interfere with binding, indicating a protein-protein interaction. In contrast, adhesion of yersiniae to the laminin-2 isoform occurred also independent of YadA expression and no binding was observed to nidogen/entactin. The results support the hypothesis that adhesion of Y. enterocolitica could contribute to pathogenicity of enteropathogenic yersiniae. Further definition of binding sites for YadA on BM proteins might allow determination of the relevance of Yersinia-BM interactions to infection.
Max-Planck-Society, Institute for Clinical Immunology, University Erlangen-Nürnberg, Germany.
A
A
Flügel
H
H
Schulze-Koops
J
J
Heesemann
K
K
Kühn
L
L
Sorokin
H
H
Burkhardt
K
K
von der Mark
F
F
Emmrich
D018829:Adhesins, Bacterial; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001485:Basement Membrane / Q000378:metabolism / Q000382:microbiology; D002241:Carbohydrates; D002417:Cattle; D004492:Edetic Acid; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007457:Iodine Radioisotopes; D007797:Laminin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011485:Protein Binding; D012492:Salts; D015008:Yersinia enterocolitica / Q000502:physiology*
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002241:Carbohydrates; D016326:Extracellular Matrix Proteins; D007457:Iodine Radioisotopes; D007797:Laminin; D008562:Membrane Glycoproteins; D012492:Salts; C064832:YadA protein, Yersinia; C029967:nidogen; D004492:Edetic Acid