Opposite effects of basic fibroblast growth factor on gonadotropin-stimulated steroidogenesis in rat granulosa cells.
In the present study, we examined the effect of basic fibroblast growth factor (bFGF) on gonadotropin-dependent estradiol and progesterone production in cultured rat immature and FSH-primed granulosa cells. Treatment with bFGF alone did not affect the biosynthesis of estradiol and progesterone in immature and FSH-primed granulosa cells. Basic FGF significantly inhibited FSH-induced estradiol and progesterone production in both immature and FSH-primed granulosa cells. In contrast, bFGF exerted a significant stimulatory effect on LH-induced estradiol and progesterone production in FSH-primed granulosa cells. Our finding that bFGF exerts opposite effects on gonadotropin-dependent steroidogenesis suggests that bFGF may play important paracrine and/or autocrine roles in the process of follicular development, ovulation, and subsequent luteinization.
Department of Obstetrics and Gynecology, Wakayama Medical College, Japan.
M
M
Yamoto
T
T
Shikone
R
R
Nakano
D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects
D016222:Fibroblast Growth Factor 2; D011374:Progesterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone