Two forms of the mb-1 gene transcript in cattle.
We have isolated two forms of bovine mb-1 cDNA clones from a cDNA library of a bovine leukemia virus-infected B-lymphoblastoid cell line. The nucleotide sequence analysis of these cDNA clones indicated that the shorter form of cDNA had a 119-bp deletion that precisely corresponded to the exon III domain of the human and murine mb-1 genes. This deletion would result in a frame shift, producing a premature translation termination leading to the lack of the entire transmembrane and cytoplasmic domains of the normal mb-1 molecule. The existence of two species of mRNA transcripts corresponding to the isolated cDNA clones were confirmed in all of the bovine leukemia cell lines examined by PCR with the use of the primers flanking the deletion site. Furthermore, the shorter mb-1 transcript was induced in normal PBMC by stimulation with mitogens or culture supernatants of bovine leukemia virus-infected cell lines. These findings indicate the presence of the truncated mb-1 gene product which may have some function different from that of the normal mb-1 gene product.
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
H Y
HY
Youn
R
R
Goitsuka
M
M
Okuda
T
T
Watari
H
H
Tsujimoto
A
A
Hasegawa
D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D051925:CD79 Antigens; D002417:Cattle; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D015698:Genomic Library; D001909:Leukemia Virus, Bovine; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011947:Receptors, Antigen, B-Cell / Q000235:genetics*
D015703:Antigens, CD; D051925:CD79 Antigens; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011947:Receptors, Antigen, B-Cell