Interferon-gamma (IFN-gamma) induces programmed cell death in differentiated human leukemic B cell lines.
Interferons (IFNs) are cytokines that exert an antiviral effect on target cells and possess immunomodulatory and antitumor properties. In this study we have investigated the effects of human recombinant IFN-gamma on human leukemic B cell lines at different stages of maturation. Our data show that in Burkitt's lymphoma RAMOS-1 B cells IFN-gamma induces a reduction of cell growth and a clonal selection via programmed cell death; in contrast, IFN-gamma treatment of KM-3 pre-B cells does not induce biochemical and morphological changes as shown by electron microscope analysis and DNA gel electrophoresis.
Istituto di Citomorfologia Normale e Patologica del CNR, Università di Chieti, Italy.
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D017209:Apoptosis / Q000187:drug effects*; D002051:Burkitt Lymphoma; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000302:isolation & purification; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology*; D015448:Leukemia, B-Cell; D008854:Microscopy, Electron; D011994:Recombinant Proteins; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D011994:Recombinant Proteins; D007371:Interferon-gamma; D004253:DNA Nucleotidylexotransferase; D013936:Thymidine