Tissue-specific targeting of retroviral vectors through ligand-receptor interactions.
The development of retroviral vectors that target specific cell types could have important implications for the design of gene therapy strategies. A chimeric protein containing the polypeptide hormone erythropoietin and part of the env protein of ecotropic Moloney murine leukemia virus was engineered into the virus. This murine virus became several times more infectious for murine cells bearing the erythropoietin receptor, and it also became infectious for human cells bearing the erythropoietin receptor. This type of tissue-specific targeting by means of ligand-receptor interactions may have broad applications to a variety of gene delivery systems.
Department of Laboratory Medicine, University of California, San Francisco 94143-0724.
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Kasahara
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Dozy
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YW
Kan
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004912:Erythrocytes / Q000737:chemistry / Q000821:virology*; D004921:Erythropoietin / Q000235:genetics / Q000378:metabolism; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics* / Q000502:physiology; D017467:Receptors, Erythropoietin / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000378:metabolism
D008024:Ligands; D017467:Receptors, Erythropoietin; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D004921:Erythropoietin