Electrostatic cell-to-cell adhesion by a non-proteolytic component in a protease preparation.
A crude protease preparation from papaya (Protease Type III, Sigma) brought about reversible adhesion between adjacent cells of a variety of species including those of lymphoid, epithelial and fibroblastic origin at low ionic strength (below 100 mM). Sensitivities of the adhesion activity to ambient ionic strength, Ca2+, Mg2+ and pH indicated that the cell-cell contact is based on the electrostatic interaction. By ion-exchanger chromatography and gel-filtration chromatography it is suggested that the adhesion molecules in the protease preparation are dimers of cationic protein of 28 kDa distinct from the proteolytic component.
Department of Physiology, Faculty of Medicine, Kyoto University, Japan.
T
T
Yamamoto
T
T
Ohno-Shosaku
E
E
Furukawa-Uenoyama
K
K
Nagata
Y
Y
Okada
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004560:Electricity; D010450:Endopeptidases / Q000302:isolation & purification / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005638:Fruit / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials; D051379:Mice; D014411:Neoplastic Stem Cells / Q000187:drug effects; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D013045:Species Specificity
D010940:Plant Proteins; D010450:Endopeptidases; D008274:Magnesium; D002118:Calcium