Evaluation of a method used to test for potential toxicity of carpet emissions.
After a private testing laboratory had reported mortality, neurotoxicity, and respiratory irritation in mice exposed to emissions from heated carpet in a modified application of the sensory irritation test (ASTM E981-84), the studies reported in this paper were conducted to evaluate the method used in testing for carpet toxicity and to see if the reported findings were reproducible. Mice were exposed head-only to offgasses generated by heating carpet (AT #3) to temperatures that ranged from 37 to 70 degrees C. Control mice were simultaneously exposed to heated air. The animals were evaluated for mortality, clinical signs and respiratory irritation. Neurotoxicity was evaluated using functional observational battery and motor activity monitoring. Pathological evaluations of organs and tissues, including the nervous system, were also conducted. The carpet samples heated to higher temperatures produced greater concentrations of total volatile organic compounds than those heated to 37 degrees C. Both carpet-exposed and control mice displayed some effects, such as body weight loss, mortality and pathological lesions, that were due to the exposure system. There was no mortality or neurotoxicity, nor were there clinical signs or pathological lesions as a result of carpet exposures. Mice exposed to carpet heated to 70 degrees C had slightly decreased respiratory rates and an increased incidence of breathing patterns indicative of sensory irritation. Therefore, none of the results reported by the private testing laboratory could be reproduced when this carpet was heated to temperatures below 70 degrees C, and slight sensory irritation was the only effect observed at the 70 degrees C test conditions. Effects from the exposure system itself made interpretation of results difficult, and concurrent controls were considered essential for interpretation of data.
Stadler
J C
JC
Haskell Laboratory for Toxicology and Industrial Medicine, E.I. du Pont de Nemours and Co., Newark, DE 19714.
Dudek
B R
BR
Kaempfe
T A
TA
Christoph
G R
GR
Hansen
J F
JF
D016902:Air Pollution, Indoor; D000818:Animals; D001273:Atmosphere Exposure Chambers; D001835:Body Weight; D004785:Environmental Pollutants / Q000633:toxicity*; D005069:Evaluation Studies as Topic; D005430:Floors and Floorcoverings; D006358:Hot Temperature; D007509:Irritants / Q000633:toxicity; D008297:Male; D051379:Mice; D009043:Motor Activity; D012119:Respiration; D018675:Toxicity Tests / Q000379:methods*
D004785:Environmental Pollutants; D007509:Irritants