Multicycle infection of hepatitis C virus in cell culture and inhibition by alpha and beta interferons.
We previously reported that a clone of a human lymphocytic cell line, HPBMa clone 10-2, supports the genome replication of hepatitis C virus (HCV). In the present study, the multicycle transmission of HCV from infected cells to new cells was demonstrated by coculture with drug-resistant cells as recipients; i.e., the system was proved productive for infectious HCV. Inhibition of viral replication by alpha and beta interferons was observed.
Department of Viral Diseases and Vaccine Control, National Institute of Health, Tokyo.
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YK
Shimizu
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Yoshikura
D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells; D017931:DNA Primers; D004351:Drug Resistance; D005814:Genes, Viral; D016174:Hepacivirus / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D006921:Hygromycin B / Q000494:pharmacology; D016898:Interferon-alpha / Q000494:pharmacology*; D016899:Interferon-beta / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008214:Lymphocytes; D008969:Molecular Sequence Data; D009355:Neomycin / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis
D017931:DNA Primers; D016898:Interferon-alpha; D017361:Viral Nonstructural Proteins; D006921:Hygromycin B; D016899:Interferon-beta; D007371:Interferon-gamma; D009355:Neomycin