[Amino acid analysis of retinal S-antigen and interphotoreceptor retinoid-binding protein].
Retinal S-antigen and interphotoreceptor retinoid-binding protein (IRBP) were purified by DEAE-Sephacel ionexchange chromatography and Con A Sepharose affinity chromatography. Amino acid analysis of the S-antigen and IRBP was carried out and compared with that presented by others. The possible reason for difference with regard to some amino acids and related problems were analyzed and discussed.
Yang
P
P
Zhongshan Ophthalmic Center, Sun Yat-Sen University of Medical Sciences, Guangzhou, China.
Li
S
S
Pan
S
S
Cao
X
X
Xie
C
C
Liang
S
S
Huang
X
X
Mao
W
W
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D000941:Antigens / Q000737:chemistry* / Q000302:isolation & purification; D019393:Arrestin; D001324:Autoantigens / Q000737:chemistry / Q000302:isolation & purification; D002417:Cattle; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D005136:Eye Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010726:Phosphodiesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D012160:Retina / Q000737:chemistry*; D012177:Retinol-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D000099214:Retinol-Binding Proteins, Interstitial
D000596:Amino Acids; D000941:Antigens; D019393:Arrestin; D001324:Autoantigens; D005136:Eye Proteins; D010726:Phosphodiesterase Inhibitors; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial