Induction of bcl-2 gene expression by intercellular information from hemopoietic supportive stromal cells to DA-1 cells.
When interleukin-3 (IL-3) dependent DA-1 cells were cultured on hemopoietic supportive stromal cells (MS-5), DA-1 cells survived and proliferated in the absence of detectable IL-3. Although IL-3 was not produced by the MS-5 cells, their production of granulocyte-macrophage colony-stimulating factor (GM-CSF) was increased when they were co-cultured with DA-1 cells. This suggests that DA-1 cells transmit signals to stromal cells that enhance growth factor(s) production. Expression of bcl-2 by DA-1 cells was induced when they were co-cultured with MS-5 cells, suggesting that DA-1 cells express bcl-2 strongly in response to a signal produced by MS-5 cells. These data indicate the existence of a two-way interaction between DA-1 cells and hemopoietic supportive stromal cells.
Department of Biology, Faculty of Science, Niigata University, Japan.
E
E
Takashita
K
K
Sugimoto
Y
Y
Adachi
K J
KJ
Mori
D000818:Animals; D002450:Cell Communication; D002455:Cell Division; D002470:Cell Survival; D017077:Culture Media, Conditioned; D015870:Gene Expression; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007377:Interleukin-3 / Q000096:biosynthesis; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2; D017154:Stromal Cells / Q000502:physiology*
D017077:Culture Media, Conditioned; D007377:Interleukin-3; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor