In-vitro antimicrobial susceptibility and beta-lactamases of ampicillin-resistant Escherichia coli in Hong Kong.
Three hundred and forty isolates of ampicillin-resistant Escherichia coli isolated from blood, bile and urine were collected during 1984 and 1988 in the Prince of Wales Hospital, Hong Kong and MICs of 21 beta-lactam antibiotics were determined. beta-Lactamases were identified by isoelectric focusing, and TEM-1 was found in 89.1% of isolates, PSE-1 in 3.2%, and OXA-1 in 2.9% of strains. Ten isolates (2.9%) possessed both TEM-1 and PSE-1. Five isolates (1.5%) produced only chromosomal enzymes and one produced an unidentified enzyme which focussed at pI 6.0. All but seven of 274 TEM-1 producers and all nine OXA-1 producers reacted with oligonucleotide probes in a colony blot technique. All isolates contained plasmids and 229 of 318 (72.0%) isolates transferred the ampicillin resistance factor. Of 42 transconjugants of TEM-1 producers selected for study, 39(92.9%) had only a single transferable plasmid bearing the TEM-1 gene.
Department of Microbiology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories.
T K
TK
Ling
D J
DJ
Lyon
A F
AF
Cheng
G L
GL
French
D000668:Ampicillin Resistance; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology; D006723:Hong Kong; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007527:Isoenzymes; D047090:beta-Lactams; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1