Mouse muscle epsilon- and gamma-containing acetylcholine receptors expressed in Xenopus laevis oocytes do not differ in their degradation half-lives.
Mouse acetylcholine receptors (AChRs) consisting of either the embryonic form (2 alpha, 1 beta, 1 delta and 1 gamma) or the adult form (2 alpha, 1 beta, 1 delta and 1 epsilon) were expressed in Xenopus laevis oocytes. As expected, the single channel conductance was approximately 52 pS and the exponential decay time constants were 2.2 and 8.2 ms for the gamma-AChR and respectively 65 pS and 0.6 and 2.8 ms for the epsilon-AChR. No difference was seen in the degradation rate between the gamma- and epsilon-containing AChRs, both having a half-life of about 5 days.
Department of Neurobiology and Behavior, Cornell University, Ithaca, NY 14853.
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Liu
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Hamill
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Salpeter
D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006207:Half-Life; D007457:Iodine Radioisotopes; D051379:Mice; D009132:Muscles / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000096:biosynthesis; D011950:Receptors, Cholinergic / Q000235:genetics / Q000378:metabolism*; D014101:Torpedo; D014158:Transcription, Genetic; D014982:Xenopus laevis
D002038:Bungarotoxins; D007457:Iodine Radioisotopes; D012333:RNA, Messenger; D011950:Receptors, Cholinergic