Angiotensin II induces human placental lactogen and pregnancy-specific beta 1-glycoprotein secretion via an angiotensin AT1 receptor.
The purpose of this work was to determine which subtype of angiotensin II receptors was involved in angiotensin II-induced human placental lactogen (hPL) and pregnancy-specific beta 1-glycoprotein (SP1) secretion from human placenta. The experiments were performed using placental explants and isolated trophoblast cells. They were pretreated with Dup753 (antagonist of angiotensin AT1 receptor), PD123319 (antagonist of AT2 subtype) or with CGP42112A (agonist of angiotensin AT2 receptor) before the addition of angiotensin II. The octapeptide angiotensin II significantly increased the hPL and SP1 release from both placental explants and isolated trophoblast cells. The angiotensin AT1 receptor antagonist Dup753 inhibited the angiotensin II-stimulated hPL and SP1 secretion in a dose-dependent manner but the AT2 specific ligands PD123319 and CGP42112A had no effect. This study indicates that the angiotensin AT1 receptor is the main mediator of angiotensin II in the stimulation of hPL and SP1 secretion from human placenta.
Department of Obstetrics and Gynecology, University of Louvain, Brussels, Belgium.
M K
MK
Kalenga
M
M
de Gasparo
K
K
Thomas
R
R
De Hertogh
D000804:Angiotensin II / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D005260:Female; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D019808:Losartan; D009842:Oligopeptides / Q000494:pharmacology; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism; D010928:Placental Lactogen / Q000378:metabolism*; D011247:Pregnancy; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000378:metabolism*; D011945:Receptors, Angiotensin / Q000187:drug effects / Q000502:physiology*; D013777:Tetrazoles / Q000494:pharmacology; D014327:Trophoblasts / Q000187:drug effects
D001713:Biphenyl Compounds; D007093:Imidazoles; D009842:Oligopeptides; D011268:Pregnancy-Specific beta 1-Glycoproteins; D011945:Receptors, Angiotensin; D013777:Tetrazoles; D000804:Angiotensin II; C060894:CGP 42112A; D010928:Placental Lactogen; D019808:Losartan