Adenosine A2B receptor signalling is altered by stimulation of bradykinin or interleukin receptors in astroglioma cells.
The human astroglioma cell D384 possesses adenosine A2B receptors coupled to the formation of cyclic AMP. These cells also possess bradykinin B2 receptors coupled to phospholipase C and consequent increases in intracellular calcium and protein kinase C. Interleukin 1 beta causes an increase in c-fos, AP-1 transcriptional activity and an increased expression of several genes including NGF, but the initial signalling events are unknown. Bradykinin causes a rapid decrease in A2B receptor mediated cAMP formation, via a mechanism that involves calcium, but not cGMP, and appears to depend upon a direct decrease in adenylyl cyclase. Il-1 beta causes a slowly developing (18-24 h) increase in A2B receptor signalling. The results indicate that adenosine effects in glial cells, believed to be important in neuroprotection, are modified in the short and long-term by inflammatory mediators.
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
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Fredholm
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Altiok
D001254:Astrocytoma / Q000378:metabolism*; D001920:Bradykinin / Q000494:pharmacology; D001932:Brain Neoplasms / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D018002:Receptors, Bradykinin / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000378:metabolism*; D018047:Receptors, Purinergic P1 / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D007378:Interleukins; D016760:Proto-Oncogene Proteins c-fos; D018002:Receptors, Bradykinin; D018123:Receptors, Interleukin; D018047:Receptors, Purinergic P1; D015544:Inositol 1,4,5-Trisphosphate; D000242:Cyclic AMP; D001920:Bradykinin; D002118:Calcium