[Rapid diagnosis of cytomegalovirus (CMV) infection in a renal transplant recipient by polymerase chain reaction (PCR)].
We used the PCR method to detect CMV-DNA in serum, urine, and peripheral blood of a patient who had received a renal transplant. A correlation was found between the active stage of the infections and the serum level of CMV-DNA. While no correlation was found in urine or blood. To detect CMV-DNA as early as possible, the serum samples were prepared by glass powder treatment and subjected to PCR followed by nonradioactive DNA hybridization. Moreover, to increase the sensitivity of detection a nested PCR method was employed and we found that the PCR-products were easily detected on the ethidium bromide-stained gel without the following hybridization. Thus, PCR in serum would be useful for rapid diagnosis of the infection and monitoring anti-viral drug therapy.
Department of Clinical Pathology, Showa University Fujigaoka Hospital, Yokohama.
T
T
Yamamoto
Y
Y
Nakajima
T
T
Hironaka
K
K
Hirai
Y
Y
Nakamura
D000328:Adult; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000097:blood*; D006801:Humans; D016867:Immunocompromised Host; D016030:Kidney Transplantation; D008297:Male; D016133:Polymerase Chain Reaction
D004279:DNA, Viral