ABL and BCR genes are not imprinted in androgenetic and gynogenetic human tissues.
In the translocation leading to the formation of the Philadelphia chromosome, the hallmark of chronic myeloid leukemia (CML), the translocated chromosome 9 (ABL), is of paternal descent whereas chromosome 22 (BCR) is of maternal origin (1). To study possible imprinting of the human ABL and BCR genes, we used human tissues exclusively endowed with their maternally (benign teratoma) or paternally (complete hydatidiform mole) inherited chromosomes. Using the sensitive PCR technique followed by northern blotting, we demonstrate here that ABL and BCR are expressed to a similar extent in androgenetic and gynogenetic human tissues, thus suggesting that ABL and BCR genes are not imprinted in these human tissues.
Department of Cellular Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
H
H
Lorberboum-Galski
S
S
Yarkoni
A
A
Nechushtan
J
J
Rachmilewitz
N
N
deGroot
A
A
Hochberg
D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D016313:Genes, abl; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000235:genetics*; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013724:Teratoma / Q000235:genetics
D017931:DNA Primers; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr