Stimulus-secretion coupling in rat parietal cells is affected by extracellular magnesium.
We investigated the effects of extracellular magnesium on stimulus-secretion coupling in isolated rat parietal cells. A high concentration of extracellular magnesium (10 mM) decreased basal and carbachol-stimulated calcium levels, whereas low magnesium levels (0.2 mM) had the opposite effect. The calcium-triggered acid secretion was influenced in the same manner. Basal and carbachol-stimulated acid secretion could be enhanced by incubation in a buffer of low magnesium concentration, whereas a high magnesium concentration totally suppressed the carbachol-induced acid secretion. These results demonstrate that magnesium plays a modulative role in calcium-dependent stimulus-secretion coupling in rat parietal cells.
Department of Medicine B, University of Münster, Germany.
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FC
Mooren
R
R
Stoll
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E
Spyrou
W
W
Beil
W
W
Domschke
D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D005744:Gastric Acid / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D010295:Parietal Cells, Gastric / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000632:Aminopyrine; D002217:Carbachol; D008274:Magnesium; D002118:Calcium