Growth-promoting effect of bacterial products from Clostridium perfringens on human keratinocytes.
Wound healing substance (WHS) from cultured Clostridium perfringens has been reported to be effective in the treatment of wounds. The effects of WHS, now named SNK-863, on proliferation and differentiation of human keratinocytes were examined. The characteristics of WHS are as follows: 1) WHS stimulates human keratinocyte growth and DNA synthesis; 2) WHS and EGF show some additive effects on human keratinocyte growth; 3) WHS does not interfere with the binding of EGF to its receptor; 4) WHS does not counteract the growth inhibitory effects of TGF-beta or vitamin D3 on human keratinocytes; 5) WHS has no significant effect on human keratinocyte differentiation. These results indicate that the growth-promoting effect of WHS on keratinocytes may contribute to the treatment of wound healing.
Osaka Medical Center, Japan.
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A
Takada
K
K
Hashimoto
K
K
Yoshikawa
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003016:Clostridium perfringens / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006133:Growth Substances / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects
D006133:Growth Substances; D010455:Peptides; D016212:Transforming Growth Factor beta; C090233:SNK 863; D004815:Epidermal Growth Factor; D004247:DNA