Occurrence of autoantibody to protein disulfide isomerase in rats with xenobiotic-induced hepatitis.
The occurrence of an autoantibody to protein disulfide isomerase (PDI) in rats after administration of various hepatotoxic drugs was investigated by immunoblotting and radioimmunoassay. An anti-PDI autoantibody was detected with high frequency in rats treated with D-galactosamine, acetaminophen with diethylmaleate, and carbon tetrachloride with diethylmaleate. The antibody-positive rate was relatively low in the groups of rats given carbon tetrachloride, acetaminophen or DL-ethionine alone. The anti-PDI antibody was not detected in rats treated with diethylmaleate alone. Although the mechanism of the production of the anti-PDI autoantibody is unclear, the occurrence of anti-PDI antibody correlated with high serum GPT activities. It is suggested that the autoantibody plays an important role in the development and persistence of drug-induced hepatitis.
Nagayama
S
S
Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Yokoi
T
T
Kawaguchi
Y
Y
Kamataki
T
T
D000410:Alanine Transaminase / Q000187:drug effects; D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D056486:Chemical and Drug Induced Liver Injury / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000187:drug effects; D007535:Isomerases / Q000276:immunology*; D008297:Male; D019704:Protein Disulfide-Isomerases; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D015262:Xenobiotics / Q000633:toxicity*
D001323:Autoantibodies; D015262:Xenobiotics; D000410:Alanine Transaminase; D007535:Isomerases; D019704:Protein Disulfide-Isomerases; D005978:Glutathione