Polymerase chain reaction mediated localization of RFLP clones to microisolated translocation chromosomes of barley.
A new strategy has been devised and used for the physical localization of genetically mapped restriction fragment length polymorphism (RFLP) clones to barley chromosomes. Morphologically distinct translocation chromosomes from synchronized root-tip meristems were microisolated and their DNA was used as a template for polymerase chain reaction with sequence-specific primers. Four RFLP clones were assigned to cytologically defined segments of chromosome 5. This related approximately one-third of the map length of linkage group 5 to approximately one-fifth of the mitotic metaphase length of chromosome 5. The technique may substantially contribute to the connection of the RFLP-based genetic linkage maps with cytological markers of the barley chromosomes.
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
A
A
Sorokin
F
F
Marthe
A
A
Houben
U
U
Pich
A
A
Graner
G
G
Künzel
D001483:Base Sequence; D002875:Chromosomes; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D008040:Genetic Linkage; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014178:Translocation, Genetic
D017931:DNA Primers