C-band-positive double minutes have kinetochore proteins.
Double minutes (DMs) have been shown generally to lack centromeres. In the present paper we report on C-band-positive DMs in Colcemid-resistant SEWA cells. These DMs were heavily labeled after in situ hybridization with mouse major satellite DNA and, furthermore, reacted positively with antikinetochore antibodies. Upon antikinetochore antibody labeling, the immunofluorescent signals of some DMs were strong, indicating that they had functional centromeres.
Department of Genetics, University of Gothenburg, Sweden.
Y
Y
Wettergren
U
U
Arnason
T
T
Haaf
A
A
Levan
G
G
Levan
D000818:Animals; D001323:Autoantibodies / Q000097:blood; D017675:CREST Syndrome / Q000097:blood / Q000276:immunology; D002503:Centromere / Q000235:genetics; D002871:Chromosome Banding; D002999:Clone Cells; D004276:DNA, Satellite; D003703:Demecolcine / Q000633:toxicity; D018432:Drug Resistance, Multiple / Q000235:genetics; D006801:Humans; D018386:Kinetochores / Q000502:physiology*; D008677:Metaphase; D051379:Mice
D001323:Autoantibodies; D004276:DNA, Satellite; D003703:Demecolcine