Heterologous expression of an active rat regulatory protein of glucokinase.
The cDNA presumed to encode the rat liver regulatory protein of glucokinase has been expressed in Escherichia coli and a partially soluble protein has been obtained. This recombinant protein was partially purified and found to have the same apparent molecular mass as the regulatory protein purified from rat liver. Like the latter, it inhibited rat liver glucokinase competitively with respect to glucose and its effect was sensitive to fructose 6-phosphate and fructose 1-phosphate.
Detheux
M
M
Laboratoire de Chimie Physiologique, Université Catholique de Louvain, Belgium.
Van Schaftingen
E
E
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005636:Fructosephosphates / Q000494:pharmacology; D005941:Glucokinase / Q000037:antagonists & inhibitors*; D005947:Glucose / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012995:Solubility
D002352:Carrier Proteins; D005636:Fructosephosphates; C499484:Gckr protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D011994:Recombinant Proteins; C080906:glucokinase regulatory protein; C027618:fructose-6-phosphate; D005941:Glucokinase; D005947:Glucose