[Determination of metabolism of steroid hormones and activity of their synthetic enzymes in murine Leydig cells by high performance liquid chromatography].
A very effective procedure was developed for separation of sex hormones of the delta 4 pathway using the microcolumn technique of high performance liquid chromatography (HPLC). A five-step gradient containing methanol, acetonitrile and water was used as a mobile phase, while Lychrosorb RP-18 with particles of 5 microns was used as a sorbent. Metabolism of steroid hormones and activity of 3 beta-steroid dehydrogenase, 17 alpha-hydroxylase, C17-20-lyase and 17-ketosteroid reductase were studied in Leydig cells of two mouse strains PT and A/He. The higher rate of steroid metabolism and of the enzymatic activity was detected in the mouse cells of PT strain as compared with those of A/He strain.
K V
KV
Svechnikov
T V
TV
Busygina
A V
AV
Osadchuk
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000446:Aldehyde-Lyases / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D007985:Leydig Cells / Q000201:enzymology* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism; D013256:Steroids / Q000378:metabolism*
D012739:Gonadal Steroid Hormones; D013256:Steroids; D003577:Cytochrome P-450 Enzyme System; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; D013254:Steroid 17-alpha-Hydroxylase; D000446:Aldehyde-Lyases
Opredelenie metabolizma steroidnykh gormonov i aktivnosti fermentov ikh sinteza v kletkakh Leĭdiga mysheĭ metodom vysokoéffektivnoĭ zhidkostnoĭ khromatografii.