[Preservation of fresh homograft using TC199 solution in rat].
The surgical replacement of human vessels using fresh allografts (homograft) has been reported as useful means. Nevertheless, little is known about preservation effect and immunological consequences using TC199 solution. In this study, defined inbred strains of rats were used. The aorta was extracted from Buffalo rats and preserved for 1, 2, and 3 weeks in TC199 solution at 4 degrees C. The preserved graft was then implanted into the Buffalo rats heterotopically, in the abdominal aorta, in the autotransplant group (AUTO) and into the Lewis rat in the allotransplant group (ALLO). The graft patency and histology including immunohistochemical stain (IgG, IgM, and C3 complement) were assessed at 2 weeks after transplantation. In AUTO, the patency was 100% with grafts 1 and 2 weeks' preservation, and 80% with 3 weeks' preservation. In the ALLO, patency was 100% with grafts with one week's preservation, 80% in those with 2 weeks' preservation and 0% those with in 3 weeks' preservation. Histology revealed that fibroblasts and endothelial cells were significantly deteriorated in 3 weeks' preservation in auto transplantation, however, they were well-maintained in 2 weeks' preservation. In the allo-transplantation group, histological deterioration began after 2 weeks preservation. Immunohistochemical staining showed significant deposits of IgG, IgM and C3 in ALLO. Significant infiltration of antibodies was seen in deteriorated tissue due to prolongation of the preservation periods. These results suggested that freshly preserved allograft was antigenic and 2 weeks preservation using TC199 solution was possible in the rat model.
Department of Cardiovascular Surgery, Heart Institute of Japan, Tokyo Women's Medical College.
M
M
Hachida
N
N
Hanayama
Y
Y
Bonkohara
T
T
Nishinaka
A
A
Ookado
H
H
Hoshi
M
M
Nonoyama
H
H
Koyanagi
D000818:Animals; D001808:Blood Vessels / Q000276:immunology / Q000637:transplantation*; D004730:Endothelium, Vascular / Q000473:pathology; D007136:Immunoglobulins / Q000378:metabolism; D051381:Rats; D011915:Rats, Inbred BUF; D011917:Rats, Inbred Lew; D012996:Solutions / Q000494:pharmacology*; D014021:Tissue Preservation; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D014654:Vascular Patency
D007136:Immunoglobulins; D012996:Solutions