Determination of the pulmonary surfactant-associated protein SP-B in amniotic fluid with a competition ELISA.
A competition ELISA was developed with polyclonal chicken antibodies, with which the surfactant-associated protein B (SP-B) can be measured in amniotic fluid. The SP-B content from 240 amniotic fluid samples taken from uncomplicated pregnancies between the 29th and 42nd week of gestation was determined. The SP-B content rose from the 33rd week of gestation onwards to a median level of 800 ng SP-B/ml amniotic fluid at the end of the pregnancy. In the 3 cases examined up to now of infants with a respiratory distress syndrome the levels lay below 300 ng SP-B/ml.
University Hospital Rudolf Virchow, Department of Obstetrics, Free University Berlin, FRG.
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I
Dilger
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G
Schwedler
J W
JW
Dudenhausen
D000653:Amniotic Fluid / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D011510:Proteolipids / Q000032:analysis*; D011663:Pulmonary Surfactants / Q000032:analysis*; D012127:Respiratory Distress Syndrome, Newborn / Q000175:diagnosis*
D011510:Proteolipids; D011663:Pulmonary Surfactants