Expression of transforming growth factor-beta 1-4 in the chick limb bud mesenchymal cells: regulation by 1,25-dihydroxyvitamin D3.
When mesenchymal cells from the early chick limb bud (stage 23-24) are plated at high cell density they spontaneously undergo chondrogenesis, implicating extensive cell-to-cell interactions. In the past it has been shown that TGF-beta or 1,25 dihydroxyvitamin D3 influences this process. In the present study we investigated the expression of TGF-beta isoforms in the mesenchymal cells after treatment with 1,25-dihydroxyvitamin D3. We found that TGF-beta 2 was down-regulated due to the treatment. The size of the transcripts was the expected as described for sternum cartilage cells but with differential use preference.
Department of Biology, University of Dayton, OH 45469-2320.
K
K
Del Rio-Tsonis
P A
PA
Tsonis
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D015536:Down-Regulation; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014807:Vitamin D / Q000494:pharmacology
D016212:Transforming Growth Factor beta; D014807:Vitamin D; D002117:Calcitriol