Saccharomyces cerevisiae recombinant Pfs25 adsorbed to alum elicits antibodies that block transmission of Plasmodium falciparum.
Antibodies to Pfs25, a cysteine-rich 25-kDa protein present on the surface of Plasmodium falciparum zygotes, can completely block the transmission of malaria parasites when mixed with infectious blood and fed to mosquitoes through a membrane feeding apparatus. Recently, a polypeptide analog, Pfs25-B, secreted from recombinant Saccharomyces cerevisiae was found to react with conformation-dependent, transmission-blocking monoclonal antibodies and to elicit transmission-blocking antibodies in experimental animals when emulsified in either Freund's or muramyl tripeptide adjuvant. In this study, Pfs25-B adsorbed to alum induced transmission-blocking antibodies in both rodents and primates. Bacterially produced Pfs25, however, did not elicit complete transmission-blocking antibodies in rodents. Furthermore, unlike monoclonal antibodies to Pfs25, which block transmission only after ookinete development, antisera to Pfs25-B adsorbed to alum appeared to block the in vivo development of zygotes to ookinetes as well.
Molecular Vaccine Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
D C
DC
Kaslow
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Bathurst
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Lensen
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Ponnudurai
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PJ
Barr
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Keister
D000276:Adjuvants, Immunologic; D000327:Adsorption; D000534:Alum Compounds; D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D016645:Aotidae; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D016778:Malaria, Falciparum / Q000517:prevention & control*; D051379:Mice; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000254:growth & development; D015800:Protozoan Proteins / Q000235:genetics / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000627:therapeutic use; D012441:Saccharomyces cerevisiae / Q000235:genetics; D015053:Zygote / Q000254:growth & development
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; C071284:Pfs25 protein, Plasmodium falciparum; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C041524:aluminum sulfate