CR1(CD35) and CR2(CD21) complement C3 receptors are expressed on normal human thymocytes and mediate infection of thymocytes with opsonized human immunodeficiency virus.
The present study demonstrates that the C3b receptor CR1 (CD35) and the C3dg/Epstein-Barr virus receptor CR2 (CD21) are expressed by 25% and 70% of normal human thymocytes, respectively. The expression of CR2 extends to both CD1+ and CD1- cells in the thymus. Two subsets of CR2+ thymocytes were defined expressing low and high density of the receptor. The CR2++ subset represented 20% of CR2+ thymocytes and co-expressed the CR1 receptor. CR2++ thymocytes expressed an immature CD1dull, CD3-, CD4dull, CD8-, CD7++ phenotype and included a subpopulation of large cells expressing CD34. Twenty percent of thymocytes expressed the CD21 epitope defined by monoclonal antibody BU32, which is involved in the binding of CD23 to CD21. These observations provide a basis for a role for CD21 in the proliferation and differentiation of thymocytes at early stages of maturation. The functionality of CR1 and CR2 on thymocytes was evidenced by the ability of the receptors to mediate infection of cells with complement-opsonized human immunodeficiency virus (HIV). The results may be relevant to the immunopathogenesis of HIV infection.
Delibrias
C C
CC
INSERM U28, Hôpital Broussais, Paris, France.
Mouhoub
A
A
Fischer
E
E
Kazatchkine
M D
MD
D002648:Child; D006678:HIV / Q000502:physiology*; D006801:Humans; D010587:Phagocytosis; D017463:Receptors, Complement 3b / Q000032:analysis* / Q000502:physiology; D017464:Receptors, Complement 3d / Q000032:analysis* / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology*
D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d