Analysis of biochemical processes in single living cells by quantitative microscopy.
Spectroscopic measurements in single living cells are made possible by the development of computer controlled light detectors, which, when applied to optical microscopes, yield spatial, temporal and eventually spectroscopic information about the sample. This minireview describes some experiments in which the distribution and concentration of specific intracellular markers (proteins, protein complexes, RNA) has been followed by quantitative microscopy. The examples chosen have contributed to shed light on a biochemical process as it happens in vivo; because of the non ideal conditions of the intracellular milieu, the comparison of in vivo and in vitro experiments is of great relevance to the understanding of cellular physiology.
Istituto Pasteur, Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche, Università La Sapienza, Roma, Italy.
A
A
Bellelli
R
R
Ippoliti
E
E
Lendaro
M
M
Brunori
D000818:Animals; D002477:Cells / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008853:Microscopy / Q000295:instrumentation*; D009836:Oligodendroglia / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism; D013053:Spectrophotometry / Q000295:instrumentation*; D008941:Spindle Apparatus / Q000378:metabolism