High pressure conditions stimulate expression of chloramphenicol acetyltransferase regulated by the lac promoter in Escherichia coli.
Recombinant plasmids with the chloramphenicol acetyltransferase (CAT) structural gene behind several kinds of promoters were tested for expression in Escherichia coli during growth at atmospheric pressure (0.1 MPa) and at high pressure (30 MPa). Expression of the CAT gene from the lac promoter was remarkably activated (approx. 78-fold) by high pressure in the absence of the inducer isopropyl-beta-D-thiogalactopyranoside (IPTG). The stimulation of the CAT activity by the lac promoter at high pressure did not simply result from an increased plasmid copy number, because the CAT activities from the other promoters and beta-lactamase activities were unaffected at high pressure.
Deep Star Group, Japan Marine Science and Technology Center, Yokosuka.
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Kato
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Sato
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Smorawinska
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Horikoshi
D001274:Atmospheric Pressure; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial / Q000502:physiology*; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000502:physiology*
D015500:Chloramphenicol O-Acetyltransferase