Interleukin-10 inhibits motility in murine and human B lymphocytes.
We here report the finding that the anti-inflammatory cytokine interleukin-10 (IL-10) inhibits motility of B lymphocytes. B cells were induced to display motile morphology and active migration by IL-4. IL-10 inhibited locomotor responses to IL-4, when B cells of both murine and human origin were used. The inhibitory effect of IL-10 was reversible, since washing of B cells preincubated in IL-10 restored the ability to respond to IL-4. Time-course experiments showed that IL-10 did not have to be present from the very onset of culture, but could be added as late as 5 hr after initiation. In addition, murine B cells stimulated with lipopolysaccharide (LPS) showed motile morphology, as well as cellular aggregation and proliferation. All these parameters were suppressed by IL-10. However, viability of B cells was not adversely affected by IL-10. Exposure to IL-10 did not result in any changes in the surface expression of molecules involved in adhesion, such as CD2, CD11a/CD18, CD44, CD54 or L-selectin, on B lymphocytes.
Department of Immunology, Stockholm University, Sweden.
B
B
Clinchy
P
P
Björck
S
S
Paulie
G
G
Möller
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000032:analysis; D002449:Cell Aggregation / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology*; D015847:Interleukin-4 / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D014066:Palatine Tonsil / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
D015815:Cell Adhesion Molecules; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D016753:Interleukin-10; D015847:Interleukin-4