Reactive oxygen product formation by human neutrophils as an early marker for biocompatibility of dialysis membranes.
Production of reactive oxygen intermediates (ROI) by neutrophils (PMN) in vivo was examined by a whole blood assay using dichlorofluorescein-diacetate (DCFH-DA) in 10 patients each dialysed consecutively with two different dialyser membranes. Haemodialysis (HD) with cuprophan membrane (CM) led to a significantly (P < 0.001) more pronounced ROI production by PMN (2.4 +/- 0.5-fold increase in intracellular oxidation of DCFH-DA) compared with HD with polysulfone membranes (PM; 1.5 +/- 0.2-fold). HD with CM induced a decrease in PMN count by about 90%, whereas PM induced a decrease by only 25% (P < 0.001). In CM patients maximal ROI production coincided with the nadir in PMN count. All patients dialysed with CM showed a clear increase in serum levels of Bb fragments, whereas PM-dialysed patients did not. In this respect, however, no clear time relationship was seen to the kinetics of ROI production, nor to the disappearance of neutrophils from the circulation. Evaluating a direct effect of the dialysis membranes on PMN demonstrated that incubation of neutrophils with hollow fibres of the CM but not of the PM in the absence of plasma induces significant ROI production by PMN. Our study thus indicates that ROI production by PMN during HD correlates to membrane biocompatibility. Furthermore, one might speculate that also independently from but perhaps in addition to complement activation, reactive oxygen products are critically involved in the generation of haemodialysis-associated neutrophil emigration.
Institute of Immunology, University of Vienna, Austria.
A R
AR
Rosenkranz
E
E
Templ
O
O
Traindl
H
H
Heinzl
G J
GJ
Zlabinger
D000328:Adult; D000368:Aged; D001672:Biocompatible Materials / Q000009:adverse effects; D002482:Cellulose / Q000009:adverse effects / Q000031:analogs & derivatives; D003167:Complement Activation / Q000187:drug effects; D051561:Complement C3 Convertase, Alternative Pathway; D003179:Complement C3b / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D015935:Complement C4b; D016207:Cytokines / Q000032:analysis; D005260:Female; D006801:Humans; D007958:Leukocyte Count / Q000187:drug effects; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D011108:Polymers / Q000009:adverse effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D006435:Renal Dialysis / Q000295:instrumentation*; D016897:Respiratory Burst / Q000502:physiology; D013450:Sulfones / Q000009:adverse effects
D001672:Biocompatible Materials; D003181:Complement C4; D016207:Cytokines; D008567:Membranes, Artificial; D010446:Peptide Fragments; D011108:Polymers; D017382:Reactive Oxygen Species; D013450:Sulfones; C017662:polysulfone P 1700; D003179:Complement C3b; D015935:Complement C4b; C032261:complement C4d; D002482:Cellulose; C009865:cuprammonium cellulose; D051561:Complement C3 Convertase, Alternative Pathway