Resistance to amphotericin B associated with defective sterol delta 8-->7 isomerase in a Cryptococcus neoformans strain from an AIDS patient.
Two Cryptococcus neoformans strains isolated from an AIDS patient were investigated, a pretreatment isolate (CN1) and a second isolate (CN3) following failure of fluconazole and amphotericin B treatment. No difference in fluconazole sensitivity, but relative resistance to amphotericin B was observed for CN3. The sterol composition of CN3 indicated a defect in sterol delta 8-->7 isomerase in this strain and depletion of ergosterol, the major sterol of the CN1.
Kelly
S L
SL
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, Sheffield University, UK.
Lamb
D C
DC
Taylor
M
M
Corran
A J
AJ
Baldwin
B C
BC
Powderly
W G
WG
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000666:Amphotericin B / Q000494:pharmacology*; D003455:Cryptococcus neoformans / Q000187:drug effects* / Q000201:enzymology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics; D013251:Steroid Isomerases / Q000235:genetics*
D000666:Amphotericin B; D013251:Steroid Isomerases