Structure of the O83-specific polysaccharide of Escherichia coli O83:K24:H31.
The polysaccharide moiety of the O83 antigen (lipopolysaccharide, LPS) consists of D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, and D-glucuronic acid in the molar ratios 1:2:1:1. Methylation analysis of the polysaccharide and derived oligosaccharides as well as one- and two-dimensional 1H and 13C NMR spectroscopy of the polysaccharide at pD 1 and 6 showed that the O83 polysaccharide has the primary structure-->6)-alpha-D-Glc p-(1-->4)-beta-D-Glc pA-(1-->6)-beta-D-Gal p-(1-->4)-beta-D- Gal p-(1-->4)-beta-D-Glc pNAc-(1-->.
Jann
B
B
Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Shashkov
A S
AS
Hahne
M
M
Kochanowski
H
H
Jann
K
K
D000942:Antigens, Bacterial / Q000737:chemistry*; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000276:immunology*; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides