Cloning and characterization of the nuclear gene encoding plastid glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa.
The single-copy nuclear gene (GapA), encoding the plastid-localized glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of the marine red alga Gracilaria verrucosa, has been cloned and sequenced. The GapA transcriptional initiation site was located 49 bp upstream of the start codon, and a putative TATA box was found 54 bp farther upstream. A spliceosomal intron was identified in the transit-peptide-encoding region in a position very similar to intron 1 of GapA and GapB of higher plants; no introns occur in the region encoding the mature protein. These observations provisionally suggest that both red algae and higher plants descend from a single ancestral photosynthetic eukaryote, i.e. that a single endosymbiotic event gave rise to red algal and higher-plant plastids.
Institute for Marine Biosciences, National Research Council of Canada, Halifax, Nova Scotia.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017343:Genes, Plant; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics; D018087:Plastids / Q000201:enzymology / Q000235:genetics*; D000461:Rhodophyta / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D010940:Plant Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases