Coexistence of neuropeptide Y and progesterone receptor in neurons of guinea-pig arcuate nucleus.
Double-label immunocytochemistry has been employed to determine whether progesterone receptor (PR)-containing neurons in the arcuate nucleus (AR) of female guinea pig contain neuropeptide Y (NPY). Animals were ovariectomized, primed by estradiol and microinjected intracerebroventricularly with colchicine to visualize NPY-immunoreactive (IR) neurons. Only a very small population of NPY-IR perikarya (2.5% approximately) were found to be also PR-IR. NPY-IR varicosities appeared in close proximity to neurons with PR-containing nuclei. This restricted coexistence provides morphological evidence that most NPY neurons in the AR are not directly regulated by progesterone.
Warembourg
M
M
INSERM U 156, Lille, France.
Deneux
D
D
Jolivet
A
A
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010052:Ovariectomy; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*
D017476:Receptors, Neuropeptide Y; D011980:Receptors, Progesterone; D004958:Estradiol; D003078:Colchicine