Effect of template size on accumulation of defective interfering RNAs in protoplasts.
A turnip protoplast system has been used to study the effects of template size and sequence on the replication and/or stability of a small defective interfering (DI) RNA associated with turnip crinkle virus. Our results indicated that as little as a single base difference in the size of the molecule in some regions, rather than the specific sequence, affected the level of DI RNA accumulating in protoplasts.
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003.
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D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis; D003673:Defective Viruses / Q000502:physiology*; D016296:Mutagenesis; D010942:Plant Viruses / Q000502:physiology*; D011523:Protoplasts / Q000821:virology; D012367:RNA, Viral / Q000096:biosynthesis*; D017384:Sequence Deletion; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014675:Vegetables / Q000821:virology*; D014779:Virus Replication
D004279:DNA, Viral; D012367:RNA, Viral