Suppressive effects of the extracts of Japanese edible seaweeds on mutagen-induced umu C gene expression in Salmonella typhimurium (TA 1535/pSK 1002) and tumor promotor-dependent ornithine decarboxylase induction in BALB/c 3T3 fibroblast cells.
Some of epidemiological data indicated that ubiquitous consumption of seaweeds in Japan may be a possible protective factor against some types of tumor. To analyse this problem, the authors studied the antimutagenic and antitumor promotion activities in methanol-soluble extracts of typical edible seaweeds which showed suppressive effects on 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indol (Trp-P-1)-induced umu C gene expression in SOS response of Salmonella typhimurium (TA 1535/pSK 1002) and 12-O-tetradecanoylphorbol-13-acetate (TPA)-dependent ornithine decarboxylase induction in BALB/c 3T3 fibroblast cells. Although eight varieties of edible seaweeds including chlorophyta, Phaenophyta and Rhodophyta showed significant antimutagenic and antipromotion activities, they expressed the activities different from each other. Among these seaweeds, Enteromorpha prolifera ('Sujiaonori' in Japanese) and Porphyra tenera ('Asakusanori') showed relatively strong suppressive activities in both antimutagenic and antipromotion assays compared with other seaweeds. These seaweeds contained considerable amounts of beta-carotene as a possible active principle with anticarcinogenic activity. This compound was partially associated with the antimutagenic activity in the seaweed extract, but did not contribute to the antipromotion activity of seaweed extract under our experimental conditions. These results strongly suggest that Japanese edible seaweeds have possible antimutagenic and antipromotion activities probably associated with antitumor activity.
Okai
Y
Y
Division of Food and Nutrition, Osaka Kun-ei Women's College, Japan.
Higashi-Okai
K
K
Nakamura
S
S
Yano
Y
Y
Otani
S
S
D016475:3T3 Cells; D000818:Animals; D016588:Anticarcinogenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D002338:Carotenoids / Q000032:analysis / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase; D004790:Enzyme Induction / Q000187:drug effects; D029968:Escherichia coli Proteins; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial; D000432:Methanol; D051379:Mice; D008807:Mice, Inbred BALB C; D009857:Oncogenes; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012624:Seaweed / Q000737:chemistry; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D013997:Time Factors; D019207:beta Carotene
D016588:Anticarcinogenic Agents; D016587:Antimutagenic Agents; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D019207:beta Carotene; D002338:Carotenoids; C078202:UmuC protein, E coli; D004259:DNA-Directed DNA Polymerase; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate; D000432:Methanol