Induced gene expression of the hypusine-containing protein eukaryotic initiation factor 5A in activated human T lymphocytes.
The hypusine-containing protein eukaryotic initiation factor 5A (eIF-5A) is a cellular cofactor critically required for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1). eIF-5A localizes in the nuclear and cytoplasmic compartments of mammalian cells, suggesting possible activities on the level of regulated mRNA transport and/or protein translation. In this report we show that eIF-5A gene expression is constitutively low but inducible with T-lymphocyte-specific stimuli in human peripheral blood mononuclear cells (PBMCs) of healthy individuals. In contrast, eIF-5A is constitutively expressed at high levels in human cell lines as well as in various human organs. Comparison of eIF-5A levels in the PBMCs of uninfected and HIV-1-infected donors shows a significant upregulation of eIF-5A gene expression in the PBMCs of HIV-1 patients, compatible with a possible role of eIF-5A in HIV-1 replication during T-cell activation.
Sandoz Research Institute, Vienna, Austria.
D
D
Bevec
H
H
Klier
W
W
Holter
E
E
Tschachler
P
P
Valent
F
F
Lottspeich
T
T
Baumruker
J
J
Hauber
D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D017931:DNA Primers / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D015658:HIV Infections / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins; D013601:T-Lymphocytes / Q000378:metabolism*; D013997:Time Factors; D000097574:Eukaryotic Translation Initiation Factor 5A
D017931:DNA Primers; D008239:Lysine; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C100028:hypusine