Interleukin-1 beta (IL-1 beta) induces transforming growth factor-beta, (TGF-beta 1) production by rat aortic smooth muscle cells.
In cultured rat aortic smooth muscle cells (RASMCs) IL-1 beta induced TGF-beta 1 mRNA expression, which was concentration (10 pM-10 nM)- and time (2-48 h) -dependent, and sensitive to cycloheximide. The maximal increase in TGF beta 1 mRNA was 4.5 times the basal level at 10 nM of IL-1 beta. A parallel increase in TGF-beta 1 protein in the culture medium of IL-1 beta-stimulated RASMCs was also observed. A detectable level of TGF-beta 1 was found 8 h after stimulation and a plateau was reached at 72 h with a maximal increase of 5-fold and was inhibited by IL-1 receptor antagonist (IL-1ra). Our data demonstrate for the first time that IL-1 beta is a potent inducer of TGF-beta 1 synthesis and release in vascular smooth muscle cells.
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406.
T L
TL
Yue
X K
XK
Wang
B
B
Olson
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G
Feuerstein
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism*; D015152:Blotting, Northern; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*
D007375:Interleukin-1; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D003513:Cycloheximide