Neurotrophic factors in the rat penis.
An intact nerve supply is essential for normal erectile function. We have undertaken a study to examine the presence and synthesis of growth factors of the penis that support neural function. Extracts were obtained from deskinned penises of Sprague-Dawley rats, aged 3, 6 and 10 weeks, representing prepubertal, pubertal and postpubertal states. Penile extracts were subjected to Northern blot analysis to evaluate expression of nerve growth factor-beta (beta-NGF)-mRNA, PC-12 bioassay to quantitate the nerve growth promoting activity and immunoassay to detect the amount of beta-NGF protein. These initial experiments showed a disproportionately abundant level of nerve growth promoting activity as compared with the levels detected with the immunoassay. The PC-12 bioassay is sensitive to both beta-NGF and fibroblast growth factors (FGFs). To further investigate these findings, the bioassay was conducted again after heparin chromatography, with beta-NGF receptor blockade, or with the addition of anti-beta-NGF, anti-basic-FGF, or anti-acidic-FGF. These studies confirmed that the abundant nerve growth promoting activity in the rat penis is due largely to basic FGF. In conclusion, the neurotrophin NGF is expressed in the rat penis at levels consistent with its expression in other peripheral tissues. Basic-FGF, on the other hand, has been detected at levels far in excess of NGF. Since erectile function is dependent on the integrity of the vascular structure and its intact innervation and since basic FGF presents as an abundant penile growth factor with both angiogenic and neurotrophic activities, basic FGF might play a significant role in erectile physiology.
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Department of Urology, Columbia University, New York, New York 10034.
Santarosa
R P
RP
Koo
H P
HP
Buttyan
R
R
Greene
L A
LA
Kaplan
S A
SA
Olsson
C A
CA
Shabsigh
R
R
D000818:Animals; D001681:Biological Assay; D002227:Carbazoles / Q000494:pharmacology; D006493:Heparin / Q000378:metabolism; D026121:Indole Alkaloids; D008297:Male; D009414:Nerve Growth Factors / Q000032:analysis* / Q000187:drug effects / Q000235:genetics; D010413:Penis / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012741:Sexual Maturation
D002227:Carbazoles; D026121:Indole Alkaloids; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D006493:Heparin; C049985:staurosporine aglycone