High-performance capillary electrophoretic analysis of inflammatory cytokines in human biopsies.
The detection of inflammatory cytokines in pathological tissue samples is important as an indicator of disease severity and persistence. Capillary zone electrophoresis (CZE) has been shown to be a good technique for the detection and measurement of tissue-bound cytokines in frozen biopsy specimens. CZE separations in polyethylene glycol-coated capillaries, performed at neutral pH and sub-ambient temperatures was found to give the most consistent and reliable results.
Phillips
T M
TM
Department of Medicine, George Washington University Medical Center, Washington, DC 20037.
Kimmel
P L
PL
D001706:Biopsy; D016207:Cytokines / Q000032:analysis*; D004586:Electrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018836:Inflammation Mediators / Q000032:analysis*; D007668:Kidney / Q000737:chemistry*; D007678:Kidney Glomerulus / Q000737:chemistry; D008163:Luminescent Measurements; D011994:Recombinant Proteins / Q000032:analysis
D016207:Cytokines; D018836:Inflammation Mediators; D011994:Recombinant Proteins