Growth-condition dependent expression of Pasteurella haemolytica A1 outer membrane proteins, capsule, and leukotoxin.
Pasteurella haemolytica, strain P1148 (biotype A, serotype 1) was grown under iron-rich and iron-restricted conditions both with and without serum, and the outer membrane protein (OMP), capsule, and leukotoxin production studied. OMPs were evaluated by SDS-PAGE and examined by immunoblot to identify antigens recognized by sera from P. haemolytica A1 convalescent and vaccinated cattle. Capsule production was evaluated using fluorescent antibody staining and rapid plate agglutination reaction. Leukotoxin production was measured by neutrophil 51Cr-release assay. Expression of specific OMPs, amount and antigenic character of capsule, and quantity of leukotoxin produced by P. haemolytica A1 varied in response to alterations in the growth media. Immunoblots indicated the immune response of convalescent calves differs from vaccinated calves, and convalescent calves produce antibodies to novel OMPs induced by growth in iron-restricted conditions.
Department of Laboratory Medicine, Kansas State University, Manhattan 66506.
D M
DM
Gatewood
B W
BW
Fenwick
M M
MM
Chengappa
D000818:Animals; D016667:Bacterial Capsules / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D003470:Culture Media / Q000737:chemistry; D005098:Exotoxins / Q000378:metabolism*; D016978:Mannheimia haemolytica / Q000254:growth & development / Q000378:metabolism*; D012766:Pasteurellosis, Pneumonic / Q000097:blood / Q000276:immunology
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D005098:Exotoxins; C018579:leukotoxin