A plasmid from the methylotrophic actinomycete Amycolatopsis methanolica capable of site-specific integration.
Amycolatopsis methanolica contains a 13.3-kb plasmid (pMEA300) which is present both in the free state and integrated at a unique genomic location. A 2.1-kb pMEA300 DNA fragment was sequenced, revealing the putative attP site and two open reading frames, xis and int, showing similarity to genes encoding excisionases and integrases, respectively.
Department of Microbiology, University of Groningen, Haren, The Netherlands.
J W
JW
Vrijbloed
J
J
Madoń
L
L
Dijkhuizen
D039903:Actinobacteria / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D019426:Integrases; D000432:Methanol / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins
D004251:DNA Transposable Elements; D014764:Viral Proteins; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C063911:excisionase; D000432:Methanol