Adenosine induces C1- efflux in endothelial cells via a pertussis toxin-sensitive G protein.
To examine the biological role of adenosine A1 receptors in bovine pulmonary artery endothelial cells, we measured intracellular Cl-concentration [Cl-]i, using 10mM 6-methoxy-N-(3-sulfopropyl) quinolinium monohydrate (SPQ). N6-cyclopentyladenosine (CPA), a selective A1 agonist, at 10(-8)M to 10(-5)M, rapidly decreased [Cl-]i by 30% to 51%, without a rapid elevation in [Ca2+]i and cyclic AMP. This reduction in [Cl-]i was completely inhibited by 10(-8)M FK453 (a selective A1 antagonist), 500ng/ml pertussis toxin (IAP), and 2.5mM N-phenylanthranilic acid (NPA) (a Cl- channel blocker). We conclude that an A1 receptor in endothelial cells activates Cl- efflux via a PTX-sensitive G protein.
Cardiovascular Research Division, Tokyo Metropolitan Geriatric Hospital, Japan.
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M
Arima
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Ueda
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Matsushita
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T
Ozawa
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H
Yamaguchi
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000502:physiology*; D007700:Kinetics; D037342:Pertussis Toxin; D011651:Pulmonary Artery; D058914:Purinergic Antagonists; D011720:Pyrazoles / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011806:Quinolinium Compounds / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D002121:Calcium Channel Blockers; D002712:Chlorides; D058914:Purinergic Antagonists; D011720:Pyrazoles; D011725:Pyridines; D011806:Quinolinium Compounds; D010566:Virulence Factors, Bordetella; D062367:ortho-Aminobenzoates; C077100:FK 453; C048599:N(6)-cyclopentyladenosine; D004533:Egtazic Acid; C052335:6-methoxy-N-(3-sulfopropyl)quinolinium; C001209:fenamic acid; D000242:Cyclic AMP; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D000241:Adenosine; D002118:Calcium