Cloning in a plasmid of an MMTV from a wild Chinese mouse: sequencing of the viral LTR.
Plasmid subcloning by conventional techniques of full length exogenous mouse mammary viruses (MMTV) has not been realized because of the involvement of host-mediated structural changes in the viral gag gene. To circumvent this problem, an alternative subcloning method, excision of phagemid (pBluescript SK) from lambda ZAP II, was successfully used to subclone a novel exogenous MMTV (JYG-MMTV) provirus fragment containing an intact gag gene. Sequence analysis revealed that the LTR of this virus is significantly different from the LTR of C3H-MMTV in the U3 region.
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912.
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Sarkar
D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015750:Genes, gag; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000821:virology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011533:Proviruses / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral