[The effect of acute poisoning of rats with carbaryl on transamination reactions with participation of alpha-ketoglutarate. II. Activity of hepatic aminotransferases].
White male Wistar rats were poisoned orally with a single carbaryl dose 474 mg/kg (1/2 LD50). The activity of aminotransferases in the cytoplasma of liver homogenates catalysing the reactions between alpha-ketoglutarate and six amino acids (cysteine, lysine, phenylalanine, leucine, asparagine and valine) after 2, 4, 24 and 72 hours from the administration of the insecticide was determined. The activity of aminotransferases was expressed as the amount of glutamic acid developing during 1 hour of incubation of liver homogenate calculated for 1 g of tissue. Glutamic acid was determined spectrophotometrically after chromatographic separation on paper. The results of the investigations demonstrated that acute poisoning with carbaryl caused a statistically significant changes in the activity of two hepatic aminotransferases. A rise was observed in the activity of the enzyme causing transamination in the reaction between alpha-ketoglutarate and phenylalanine 24 hours after insecticide administration (64.1%), and a fall of the activity of the aminotransferase catalyzing the reaction between alpha-ketoglutarate and asparagine in the 4th, 24th and 72nd hours after intoxication (16.4%, 17.3% and 19.2% respectively).
Katedry i Zakładu Chemii Toksykologicznej Akademii Medycznej, Lublinie.
K
K
Jeleniewicz
H
H
Maciejewska-Kozak
S
S
Szczepaniak
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D012721:Carbaryl / Q000506:poisoning*; D007656:Ketoglutaric Acids / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000637:Transaminases / Q000378:metabolism*
D000596:Amino Acids; D007656:Ketoglutaric Acids; D000637:Transaminases; D012721:Carbaryl
Wpływ ostrego zatrucia szczurów karbarylem na reakcje transaminacji z udziałem alfa-ketoglutaranu. Cz. II. Aktywność aminotransferaz watrobowych.