Analysis of urinary and biliary metabolites of (+)-4-[4-(4-methylphenyl)phenylmethoxy-1-piperidinyl]butyric acid in rats by liquid chromatography-frit-fast atom bombardment mass spectrometry.
The urinary and biliary metabolites of (+)-4-[4-(4-methylphenyl)phenylmethoxy-1-piperidinyl]butyric acid [(+)-MPPB] were examined in rapid-metabolizing (RM) and slow-metabolizing (SM) male Sprague-Dawley rats by means of liquid chromatography-frit-fast atom bombardment mass spectrometry. In the RM-phenotyped rats, unchanged (+)-MPPB could not be detected in urine or bile, but 4-carboxyphenyl-MPPB was detected in bile. In the SM-phenotyped rats, unchanged (+)-MPPB was detected in bile and unchanged (+)-MPPB and beta-oxidized MPPB in urine. Thus, the inter-individual difference in (+)-MPPB metabolism in rats was confirmed in vivo.
Research and Development Division, Hokuriku Seiyaku Co., Fukui, Japan.
E
E
Takahara
O
O
Nagata
H
H
Kato
S
S
Ohta
M
M
Hirobe
D000818:Animals; D001559:Benzhydryl Compounds / Q000032:analysis* / Q000378:metabolism / Q000652:urine; D001646:Bile / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*
C090637:4-(4-(4-methylphenyl)phenylmethoxy-1-piperidinyl)butyric acid; D001559:Benzhydryl Compounds