Preferential binding of cross-reactive group A streptococcal anti-DNA monoclonal antibodies to decondensed human sperm DNA.
Four murine anti-streptococcal monoclonal antibodies (mAbs) that cross-react with human DNA were evaluated by immunofluorescence flow cytometry for their reactivity with condensed and decondensed human sperm nuclei. All 4 anti-DNA mAbs reacted with condensed sperm nuclei. The reactivity of 3 of these mAbs with decondensed sperm nuclei was 13 to 177 times higher than that found with condensed nuclei. Under identical conditions, mAbs to cytoskeletal/cytocontractile proteins lacked reactivity with decondensed sperm nuclei. Binding of monoclonal anti-DNA antibodies to decondensed sperm nuclei was abolished by preincubation with double-stranded DNA. The preferential binding of anti-DNA antibodies to decondensed sperm DNA suggests the utility of decondensed sperm nuclei as the antigenic substrate for screening anti-DNA antibodies by flow cytometry.
D'Cruz
O J
OJ
University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Dunn
T S
TS
Haas
G G
GG
Reshef
E
E
Cunningham
M W
MW
D000595:Amino Acid Sequence; D000974:Antibodies, Antinuclear / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D003429:Cross Reactions / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D013094:Spermatozoa / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*
D000974:Antibodies, Antinuclear; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal