Isolation and quantitation of long-chain acyl-coenzyme A esters in brain tissue by solid-phase extraction.
Long-chain acyl-CoA's are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and determination of acyl-CoA's. The analysis includes solid-phase extraction by an oligonucleotide purification cartridge and HPLC measurements using a synthetic internal standard. Estimates of concentration in rat brain are oleoyl-CoA (11.0 nmol/g), palmitoyl-CoA (6.0 nmol/g), stearoyl-CoA (4.0 nmol/g), and linoleoyl- and arachidonoyl-CoA (2.0 nmol/g) for a total concentration of 23 nmol/g brain.
Deutsch
J
J
Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, Maryland 20892.
Grange
E
E
Rapoport
S I
SI
Purdon
A D
AD
D000214:Acyl Coenzyme A / Q000032:analysis* / Q000302:isolation & purification*; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010171:Palmitoyl Coenzyme A / Q000032:analysis / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley
D000214:Acyl Coenzyme A; C026563:arachidonyl-coenzyme A; C017585:oleoyl-coenzyme A; D010171:Palmitoyl Coenzyme A; C017725:stearoyl-coenzyme A; C026562:linoleoyl-coenzyme A