[Functional determination of C1-inhibitor in plasma with a low level of prekallikrein].
The method of assessment of the C1-inhibitor (C1-INH) based on activation of plasma precallicrein (PC) by the fragment of Hageman's factor (HFf) was described in a previous publication (6). It was recommended as an alternative of already described methods used for assessment of C1-INH. To define the reliability of the recommended method in plasma with a reduced PC level the authors used plasma of mothers where during childbirth as a rule a steep drop of this proenzyme occurs. In a group of 23 parturient women the plasma PC levels were assessed and were within the range from 0.067-0.397 U/ml incl. only 6 plasma specimens where the PC level varied within the range of normal donors, i.e. 0.319-0.438 U/ml (mean value mean = 0.369 +/- 0.035). The mean value in plasma of the mothers was 0.237 +/- 0.100. In both groups the C1-INH was assessed by the recommended method and by the method of callicrein inactivation. In the group of mothers the results of C1-INH correlated with estimations in plasma where the PC level was within the range from 42.5 to 107.6% of the mean PC value of donors (r = 0.82). In plasmas of mothers whose levels varied from 18.2 to 50.2% of the mean value of PC donors the assessment of C1-INH by the method of HF fragment activation gave lower results in all instances. The dependence of C1-INH estimation on the amount of PC was demonstrated on deficient plasma with a defined PC level.(ABSTRACT TRUNCATED AT 250 WORDS)
Ustav hematologie a krevní transfuze, Praha.
E
E
Simonianová
H
H
Tomásová
P
P
Zoban
A
A
Mydlilová
D003174:Complement C1 Inactivator Proteins / Q000032:analysis*; D005260:Female; D006403:Hematologic Tests / Q000379:methods; D006801:Humans; D007743:Labor, Obstetric / Q000097:blood; D011247:Pregnancy; D011288:Prekallikrein / Q000032:analysis*
D003174:Complement C1 Inactivator Proteins; D011288:Prekallikrein
Funkcní stanovení C1-inhibitoru v plazmách se snízenou hladinou prekalikreinu.