TH1/TH2 subset analysis. I. Establishment of criteria for subset identification in PBMC samples from nonhuman primates.
Cloned T-cell lines from two nonhuman primate species were phenotyped and their mRNA analyzed for cytokine profiles by RT-PCR procedures. PBMC from rhesus macaques showed a relatively high frequency of cloned T-cell lines with a TH1-like profile; PBMC from sooty mangabeys showed a relatively high frequency of TH2-like cloned T-cell lines. In vitro activated macaque PBMC also resulted in a high frequency of CD4+, CD8+ dual marked cells. These findings suggest that cytokine analysis of cloned T-cell lines from nonhuman primates provides a means to distinguish subsets of both CD4+ and CD8+ T cells.
Department of Pathology, School of Medicine, Emory University, Atlanta, GA.
A A
AA
Ansari
A
A
Mayne
D
D
Hunt
J B
JB
Sundstrom
F
F
Villinger
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016665:Cercocebus atys / Q000276:immunology*; D002999:Clone Cells; D016207:Cytokines / Q000096:biosynthesis*; D006968:Hypersensitivity, Delayed; D016130:Immunophenotyping; D008253:Macaca mulatta / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D013045:Species Specificity; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D016207:Cytokines; D012333:RNA, Messenger