Oligosaccharide ligands for NKR-P1 protein activate NK cells and cytotoxicity.
A diversity of high-affinity oligosaccharide ligands are identified for NKR-P1, a membrane protein on natural killer (NK) cells which contains an extracellular Ca(2+)-dependent lectin domain. Interactions of such oligosaccharides on the target cell surface with NKR-P1 on the killer cell surface are crucial both for target cell recognition and for delivery of stimulatory or inhibitory signals linked to the NK cytolytic machinery. NK-resistant tumour cells are rendered susceptible by preincubation with liposomes expressing NKR-P1 ligands, suggesting that purging of tumour or virally infected cells in vivo may be a therapeutic possibility.
Bezouska
K
K
Glycobiology Group, Northwick Park Hospital, Harrow, Middlesex, UK.
Yuen
C T
CT
O'Brien
J
J
Childs
R A
RA
Chai
W
W
Lawson
A M
AM
Drbal
K
K
Fiserová
A
A
Pospísil
M
M
Feizi
T
T
D000818:Animals; D000954:Antigens, Surface / Q000502:physiology*; D002240:Carbohydrate Sequence; D003602:Cytotoxicity, Immunologic / Q000502:physiology*; D006017:Glycolipids / Q000276:immunology; D007694:Killer Cells, Natural / Q000502:physiology*; D037102:Lectins / Q000502:physiology*; D037181:Lectins, C-Type; D008213:Lymphocyte Activation / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009844:Oligosaccharides / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D011971:Receptors, Immunologic / Q000502:physiology*; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D006017:Glycolipids; D037102:Lectins; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009844:Oligosaccharides; D011971:Receptors, Immunologic