Enhancement of plasma levels of tumor necrosis factor alpha but not interleukin-6 following endotoxin injection of Friend leukemia virus infected mice.
Friend leukemia virus complex (FLC) infection of BALB/c mice causes a rapid, progressive suppression of most immune functions. In the present study, FLC infection resulted in increased induction by bacterial lipopolysaccharide (LPS) of tumor necrosis factor alpha (TNF alpha) but not IL-6. TNF alpha levels were significantly elevated beginning 11 days post infection and increasing levels were measured through day 21. The highest TNF alpha levels in FCL-infected mice were as much as 100-fold higher than in LPS treated non-infected mice. Peak plasma levels of TNF alpha were seen between 1 and 2 hr after LPS induction, as compared to a peak at 1 hr in controls. The ability of LPS to stimulate TNF alpha was concentration dependent over a range of 0.005 to 50 micrograms per mouse. Using anti-TNF alpha antiserum, cytotoxic activity of plasma was shown to be due specifically to TNF alpha. These data suggest that induction of TNF alpha and IL-6 is regulated by different mechanisms in FLC-infected mice.
Department of Biomedicine, University of Pisa, Italy.
E
E
Soldaini
F
F
Baldinotti
D
D
Matteucci
S
S
Specter
M
M
Bendinelli
D000818:Animals; D005260:Female; D005622:Friend murine leukemia virus; D015850:Interleukin-6 / Q000097:blood*; D007700:Kinetics; D007942:Leukemia, Experimental / Q000276:immunology*; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D012192:Retroviridae Infections / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D014412:Tumor Virus Infections / Q000276:immunology*
D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha