Effects of near UV radiation and antioxidants on the response of dogfish (Mustelus canis) lens to elevated H2O2.
In vitro exposure of dogfish (Mustelus canis) lenses to near-UV energy not incompatible with that of the environment, causes an opalescence that is not present in unirradiated lenses or those irradiated after soaking in alpha-tocopherol or deferoxamine. The ability of whole lenses to destroy H2O2, as shown by their ability to produce O2 bubbles in H2O2 containing media, is markedly diminished by UV exposure without these antioxidant/free radical scavengers added. The lens capsule epithelium is the major site of catalase activity. Bubble formation was prevented by presoaking the lenses in 3-amino-triazole (3-AT), a potent catalase inhibitor. Analytical measurements confirmed the above observations. Near-UV inhibition of catalase may allow H2O2 in the aqueous humor to damage the lens by exerting oxidative stress.
Ophthalmic/Biochemistry Laboratory, University of Rochester School of Medicine, NY 14642.
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Zigman
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Rafferty
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D003676:Deferoxamine / Q000494:pharmacology; D004284:Dogfish; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D014810:Vitamin E; D006861:Hydrogen Peroxide; D002374:Catalase; D003676:Deferoxamine