In vitro effect of 5-fluorouracil, verapamil and hyperthermia on the human pancreatic adenocarcinoma cell line ASPC-1.
The purpose of this study was to assess the efficacy of verapamil (20 microM) and hyperthermia (42 degrees C) as modifiers of 5-fluorouracil (5-FU) used at different concentrations, in inhibiting the growth of human pancreatic adenocarcinoma cells. Combined verapamil and hyperthermia treatment significantly decreased cell count by 63.8% when compared to the control. Verapamil drastically enhanced the growth inhibitory effect of 5-FU at all concentrations. At high-concentration 5-FU (50 micrograms/ml), verapamil and hyperthermia had a synergistic growth-inhibitory effect and caused a significant decrease in cell count by 81.4% in comparison to the control. The modalities analyzed in this study require further investigation and have potential for clinical applicability to pancreatic cancer therapy in the future.
Lombardi Cancer Center, Georgetown University, Washington, D.C.
I B
IB
Shchepotin
R R
RR
Buras
R J
RJ
Nauta
M
M
Shabahang
V A
VA
Soldatenkov
S R
SR
Evans
D000230:Adenocarcinoma / Q000473:pathology* / Q000628:therapy; D002452:Cell Count / Q000187:drug effects; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D006979:Hyperthermia, Induced; D010190:Pancreatic Neoplasms / Q000473:pathology* / Q000628:therapy; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014700:Verapamil / Q000494:pharmacology*
D014700:Verapamil; D005472:Fluorouracil