Studies on thermophile products. VII. Effect of 1,3-di-14-methylpentadecanoyl glycerol and its related isofatty acids on T cell proliferation in vitro.
It has been found that Bacillus stearothermophilus UK563-derived immunosuppressant fraction (Fr. 5-B) consists of 1,3-diacylglycerols with saturated iso- and anteiso-type fatty acids (C14:0-C18:0) as major components. The compound, 1,3-di-14-methylpentadecanoyl glycerol (1,3-diiso C16:0 G), was synthesized and its effect on T cell proliferation was investigated together with its related isofatty acids. While 1,3-diiso C16:0 G, iso C16:0, iso C17:0, iso C17:0 methyl ester (OMe) and anteiso C17:0 OMe suppressed the mixed lymphocyte reaction (MLR) of C57BL/6 against BALB/c mice, iso C15:0, 1,3-acylglycerols with normal C16:0, C16:1 and C18:0 did not, suggesting that the presence of isofatty acids with a certain length may be essential for the suppression of MLR. 1,3-Diiso C16:0 G and iso C16:0 strongly inhibited the autologous MLR of mesenteric lymph node cells against self-antigen presenting cells in MRL/MpJ-lpr/lpr (MRL/lpr) mice, but had no effect on concavalin A-induced T cell proliferation.
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Y
Y
Kohama
K
K
Tanaka
S
S
Takae
T
T
Semba
H
H
Tone
S
S
Itoh
A
A
Inada
T
T
Nakanishi
T
T
Mimura
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A; D004075:Diglycerides / Q000378:metabolism / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013237:Stereoisomerism; D013601:T-Lymphocytes / Q000187:drug effects*
C090384:1,3-bis(14-methylpentadecanoyl)glycerol; D004075:Diglycerides; D005227:Fatty Acids; D003208:Concanavalin A