Polyribosome targeting to microtubules: enrichment of specific mRNAs in a reconstituted microtubule preparation from sea urchin embryos.
A subset of mRNAs, polyribosomes, and poly(A)-binding proteins copurify with microtubules from sea urchin embryos. Several lines of evidence indicate that the interaction of microtubules with ribosomes is specific: a distinct stalk-like structure appears to mediate their association; ribosomes bind to microtubules with a constant stoichiometry through several purification cycles; and the presence of ribosomes in these preparations depends on the presence of intact microtubules. Five specific mRNAs are enriched with the microtubule-bound ribosomes, indicating that translation of specific proteins may occur on the microtubule scaffolding in vivo.
Hamill
D
D
Department of Physiology and Cell Biology, University of Kansas, Lawrence 66045.
Davis
J
J
Drawbridge
J
J
Suprenant
K A
KA
D000818:Animals; D001755:Blastocyst / Q000502:physiology* / Q000648:ultrastructure*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D005306:Fertilization; D008297:Male; D008854:Microscopy, Electron; D008868:Microtubule Proteins / Q000032:analysis / Q000096:biosynthesis*; D008870:Microtubules / Q000502:physiology* / Q000648:ultrastructure; D010063:Ovum / Q000166:cytology / Q000502:physiology; D039102:Poly(A)-Binding Proteins; D011132:Polyribosomes / Q000502:physiology* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D012617:Sea Urchins / Q000196:embryology; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology
D008868:Microtubule Proteins; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins