Comparison of pig, rabbit and mouse IgG response to Streptococcus suis serotype 2 proteins and active immunization of mice against the infection.
The aim of this study was to compare the IgG response of different animal species to Streptococcus suis serotype 2 proteins and to evaluate the immunogenic potential of these proteins in the mouse experimental model of infection. The protein profiles of ten different S. suis capsular type 2 isolates were compared by Western blotting using antisera produced in mice, rabbits and pigs against the reference strain. Strains were grown overnight in Todd-Hewitt broth, harvested by centrifugation, processed in a French press cell and digested with lysozyme. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was then performed and proteins transferred to nitrocellulose. The rabbit antiserum recognized seventeen common immunoreactive proteins, of which, proteins of 33, 44, 96, 122 kDa were present in all strains. Two, 128 and 136 kDa proteins were recognized by swine serum in many strains. An additional protein of 30 kDa was recognized by the mouse antiserum. These seven proteins, originating from the reference strain, were excised directly from polyacrylamide gels, mixed with incomplete Freund's adjuvant and given to groups of five mice on days 0 and 10. Immunoglobulin G response to each protein was monitored on day 20 using Western blots. Mice were then experimentally infected on day 21. Results indicated that vaccination with proteins of 33, 44, 128 and 136 kDa resulted in an IgG response and protection against the challenge with the reference strain, but gave only a partial protection against another virulent S. suis serotype 2 strain.
Groupe de Recherche sur les Maladies Infectieuses du Porc. Faculté de Médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
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Quessy
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Dubreuil
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Caya
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Létourneau
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Higgins
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000097:blood*; D051379:Mice; D011817:Rabbits; D013045:Species Specificity; D013290:Streptococcal Infections / Q000097:blood / Q000276:immunology* / Q000517:prevention & control; D017011:Streptococcus suis / Q000276:immunology*; D013552:Swine; D014611:Vaccination
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G