Cloning and sequencing of a rat heart cDNA encoding a G-protein beta subunit related to the human retinal beta 3 subunit.
A cDNA (pJ5) encoding a protein with significant sequence identity to the previously known beta 1, beta 2, beta 3 and beta 4 subunits of guanine-nucleotide-binding proteins (G proteins) has been cloned from a 8-12-week-old rat heart cDNA library. Its predicted amino acid (aa) sequence of 340 aa most closely resembles that of the human beta 3 subunit, showing 96% identity. Northern blot analysis revealed that the major mRNA corresponding to the rat pJ5 cDNA is 2.0 kb in length. It is expressed at a high level in the heart and at a much lower level in the brain.
Ray
K
K
Weis Center for Research, Geisinger Clinic, Danville, PA 17822.
Robishaw
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000096:biosynthesis; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats / Q000235:genetics*; D012172:Retinaldehyde / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D046911:Macromolecular Substances; D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D012172:Retinaldehyde