Separations of basic amino acid benzyl esters by pH-zone-refining counter-current chromatography.
pH-Zone-refining counter-current chromatography was applied to the separation of basic amino acid benzyl esters. The method uses a retainer base in the stationary phase to retain analytes in the column and an eluent acid to elute the analytes in the decreasing order of pKa and hydrophilicity. The preparative capability of the method is demonstrated in the separation of a 10-g quantity of the sample.
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Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
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D000596:Amino Acids / Q000302:isolation & purification*; D001565:Benzoates / Q000302:isolation & purification*; D002845:Chromatography; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D001565:Benzoates; D007202:Indicators and Reagents; D012997:Solvents