Immunization with whole inactivated vaccine protects from infection by SIV grown in human but not macaque cells.
Homologous SIVsm stocks were generated by passage of a macaque isolate of SIVsm (SIVsm/E660) in human CEM x 174 cells or macaque peripheral blood mononuclear cells. Macaques were immunized with whole inactivated SIV vaccine consisting of virus generated by transfection of CEM x 174 cells with the SIVsmH4 clone and were challenged with either cell-free stock. Only vaccinees challenged with virus generated in human cells were protected from infection. This confirms the species-specificity of whole inactivated vaccine-mediated protection.
Goldstein
S
S
Immunodeficiency Viruses Section, NIAID, NIH, Rockville, MD 20852.
Elkins
W R
WR
London
W T
WT
Hahn
A
A
Goeken
R
R
Martin
J E
JE
Hirsch
V M
VM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008253:Macaca mulatta; D018449:SAIDS Vaccines / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000517:prevention & control; D015302:Simian Immunodeficiency Virus / Q000254:growth & development / Q000276:immunology*; D013997:Time Factors; D014766:Viremia / Q000276:immunology / Q000517:prevention & control
D000914:Antibodies, Viral; D018449:SAIDS Vaccines