Localization of a kinesin-related protein to the central pair apparatus of the Chlamydomonas reinhardtii flagellum.
Affinity-purified polyclonal antibodies raised against the conserved motor domain of Drosophila kinesin (alpha HD) recognized a 110 kDa component of the Chlamydomonas flagellar axoneme. Whole-mount immunogold labeling of splayed axonemes showed the striking localization of this antigen along one of the two microtubules of the central pair apparatus. Interestingly, the alpha HD antigen was also localized along the central axis of mutant axonemes lacking the central pair microtubules. These results suggest that a 110 kDa kinesin-related protein is a structural component of the flagellar central pair apparatus and that it is correctly targeted even in the absence of the central pair microtubules.
Department of Biology, Haverford College, PA 19041.
K A
KA
Johnson
M A
MA
Haas
J L
JL
Rosenbaum
D000595:Amino Acid Sequence; D000818:Animals; D002696:Chlamydomonas / Q000737:chemistry / Q000648:ultrastructure*; D004330:Drosophila / Q000276:immunology; D005407:Flagella / Q000737:chemistry* / Q000648:ultrastructure; D007150:Immunohistochemistry; D016547:Kinesins / Q000276:immunology; D016253:Microscopy, Immunoelectron; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000276:immunology; D008870:Microtubules / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis* / Q000276:immunology; D015800:Protozoan Proteins / Q000032:analysis* / Q000276:immunology
D008869:Microtubule-Associated Proteins; D010446:Peptide Fragments; D010940:Plant Proteins; D015800:Protozoan Proteins; D016547:Kinesins