A physical map and candidate genes in the BRCA1 region on chromosome 17q12-21.
We have constructed a physical map of a 4 cM region on chromosome 17q12-21 that contains the hereditary breast and ovarian cancer gene BRCA1. The map comprises a contig of 137 overlapping yeast artificial chromosomes and P1 clones, onto which we have placed 112 PCR markers. We have localized more than 20 genes on this map, ten of which had not been mapped to the region previously, and have isolated 30 cDNA clones representing partial sequences of as yet unidentified genes. Two genes that lie within a narrow region defined by meiotic breakpoints in BRCA1 patients have been sequenced in breast cancer patients without revealing any deleterious mutations. These new reagents should facilitate the identification of BRCA1.
Eccles Institute of Human Genetics, University of Utah, Salt Lake City 84112.
H M
HM
Albertsen
S A
SA
Smith
S
S
Mazoyer
E
E
Fujimoto
J
J
Stevens
B
B
Williams
P
P
Rodriguez
C S
CS
Cropp
P
P
Slijepcevic
M
M
Carlson
D001324:Autoantigens; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009124:Muscle Proteins; D009857:Oncogenes; D010051:Ovarian Neoplasms / Q000235:genetics*; D046988:Proteasome Endopeptidase Complex
D001324:Autoantigens; D017931:DNA Primers; D004273:DNA, Neoplasm; D005819:Genetic Markers; C099558:Ki antigen; D009124:Muscle Proteins; D046988:Proteasome Endopeptidase Complex