Improvement of penicillin G acylase production by using pac gene clone.
The penicillin G acylase gene (pac gene) from Escherichia coli ATCC 9637 has been isolated. It conferred the production of penicillin G acylase upon E. coli HB101 and other enteric bacilli. Restriction enzyme analysis and subcloning studies reveal that the gene is contained within a 2.3 kb HindIII-SmaI DNA fragment. In vitro protein synthesis study suggests a gene product of approximately 90 kDa. By using the genetically engineered bacteria which harbor a novel recombinant plasmid (pGL5) bearing a constitutively expressible pac gene, a 20-fold increased production yield of penicillin G acylase activity was obtained as compared with that produced by the original strain, E. coli ATCC 9637.
Liu
Y T
YT
Institute of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Ji
D D
DD
Chou
C C
CC
Chao
H Y
HY
Liao
C L
CL
Han
S H
SH
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D010399:Penicillin Amidase / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; D010399:Penicillin Amidase