High-performance liquid chromatographic measurement of the novel anti-HIV agent 7,8-dihydrocostatolide (NSC 661123).
An HPLC assay was used to determine levels of a promising anti-HIV agent 7,8-dihydrocostatolide (DC; NSC 661123) in human and murine plasma. The structurally related compound costatolide (C) was found to be a suitable internal standard. Drug was extracted from human or murine plasma using a solid-phase C18 cartridge. The compound was eluted from an ODS analytical HPLC column using an acetonitrile-water mobile phase. Drug was quantified over the assay range of 19.5 to 625 ng/ml with excellent within- and between-day reproducibility. Data resulting from the use of the assay method for determination of dihydrocostatolide pharmacokinetics in mice are presented. This is the first report of a validated HPLC assay for determining DC levels in human and mouse plasma.
University of Texas, M.D. Anderson Cancer Center, Department of Clinical Investigation, Houston 77030.
R A
RA
Newman
M
M
Costa
A
A
Cisneros
D000818:Animals; D000998:Antiviral Agents / Q000097:blood*; D001578:Benzopyrans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006678:HIV / Q000187:drug effects*; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D011786:Quality Control; D015203:Reproducibility of Results
D000998:Antiviral Agents; D001578:Benzopyrans; C090636:dihydrocostatolide