Method for the identification of saxitoxin in rat urine.
Saxitoxin (STX) is one of several related toxins that cause paralytic shellfish poisoning. We used solid-phase extraction (SPE) and prechromatographic oxidation/HPLC with fluorescence detection to isolate, identify, and quantify STX in rat urine. STX recovery from urine with the SPE procedure was approximately 76 +/- 6.5%. The standard curve was linear between 2 and 50 ng/ml. The lower limit of quantification with the method was 2 ng STX/ml of rat urine. Preliminary results with i.v. administration of STX to rats demonstrated that this method can detect and quantify STX in urine.
Stafford
R G
RG
Toxinology Division, United States Army Medical Research Institute of Infectious Diseases, Ft. Detrick, Frederick, MD 21702-5011.
Hines
H B
HB
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D008832:Microchemistry; D010084:Oxidation-Reduction; D051381:Rats; D012530:Saxitoxin / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine*
D012530:Saxitoxin