[HPLC analysis of 2-(P-dimethylaminostyryl) pyridine methiodide concentration in plasma].
A reversed phase high performance liquid chromatography (RP-HPLC) method for determination of 2-(p-dimethylaminostyryl) pyridine methiodide (DSPM) concentration in rabbit plasma is reported. The stationary phase was YWG-C18H37. The mobile phase consisted of 80% CH3OH, 20% CH3COOH-CH3COONa buffer (pH 4.0, 0.20 mol/L) and (C2H5)2NH 25 mmol/L. Its flow rate was 1.0 ml/min. The column temperature and pressure was 28 degrees C and 10.13 MPa, respectively. The detect wavelength was 460 nm. The detection limit of DSPM was 2 ng or 0.08 mumol/L in plasma. The RSD (%) of within-day and between-days were less than 7%. A good linear relationship between peak area and DSPM concentration in plasma (0.68-13.65 mumol/L) was found (r = 0.9996).
Department of Pharmacology, Urumqi General Hospital of Lanzhou Military Command, PLA.
J W
JW
Li
P
P
Zeng
K J
KJ
Zhang
D000818:Animals; D002121:Calcium Channel Blockers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D011726:Pyridinium Compounds / Q000097:blood*; D011817:Rabbits
D002121:Calcium Channel Blockers; D011726:Pyridinium Compounds; C011786:2-(4-(dimethylamino)styryl)-1-methylpyridinium