Effect of dietary n-3 and n-6 polyunsaturated fatty acids on lipid-metabolizing enzymes in obese rat liver.
This study was designed to examine whether n-3 and n-6 polyunsaturated fatty acids at a very low dietary level (about 0.2%) would alter liver activities in respect to fatty acid oxidation. Obese Zucker rats were used because of their low level of fatty acid oxidation, which would make increases easier to detect. Zucker rats were fed diets containing different oil mixtures (5%, w/w) with the same ratio of n-6/n-3 fatty acids supplied either as fish oil or arachidonic acid concentrate. Decreased hepatic triacylglycerol levels were observed only with the diet containing fish oil. In mitochondrial outer membranes, which support carnitine palmitoyltransferase I activity, cholesterol content was similar for all diets, while the percentage of 22:6n-3 and 20:4n-6 in phospholipids was enhanced about by 6 and 3% with the diets containing fish oil and arachidonic acid, respectively. With the fish oil diet, the only difference found in activities related to fatty acid oxidation was the lower sensitivity of carnitine palmitoyltransferase I to malonyl-CoA inhibition. With the diet containing arachidonic acid, peroxisomal fatty acid oxidation and carnitine palmitoyltransferase I activity were markedly depressed. Compared with the control diet, the diets enriched in fish oil and in arachidonic acid gave rise to a higher specific activity of aryl-ester hydrolase in microsomal fractions. We suggest that slight changes in composition of n-3 or n-6 polyunsaturated fatty acids in mitochondrial outer membranes may alter carnitine palmitoyltransferase I activity.
Laboratoire de Nutrition Cellulaire et Métabolique, Université de Bourgogne, Faculté des Sciences Mirande, Dijon, France.
I
I
Niot
J
J
Gresti
J
J
Boichot
G
G
Semporé
G
G
Durand
J
J
Bézard
P
P
Clouet
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D050356:Lipid Metabolism; D008297:Male; D008316:Malonyl Coenzyme A / Q000494:pharmacology; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000378:metabolism*; D008995:Monoamine Oxidase / Q000378:metabolism*; D009765:Obesity / Q000201:enzymology; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D051381:Rats; D011924:Rats, Zucker; D013347:Subcellular Fractions / Q000378:metabolism; D014503:Urate Oxidase / Q000378:metabolism*
D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D010171:Palmitoyl Coenzyme A; D008316:Malonyl Coenzyme A; D008995:Monoamine Oxidase; D014503:Urate Oxidase; D002334:Carnitine O-Palmitoyltransferase; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase