Enhancement of cytotoxic agent accumulation by Doppler flow sonication of human ovarian cancer cells.
The intracellular accumulation of adriamycin (ADR) by Doppler flow sonication was investigated in a human ovarian carcinoma line. Cytofluorometry was performed on cells treated by ADR (0.5, 1 and 2 micrograms/mL) alone, or in conjunction with Doppler flow sonication for 5, 10, and 15 minutes. Increased intracellular accumulation of ADR was observed in cells treated at each of these test intervals. Statistically significant increases in high permeability, due to Doppler sonication, were around 1.5 fold at each of the test intervals, with a maximum observed at 10 minutes of treatment. Examination of ADR dose effect showed that the most vigorous to Doppler sonication occurred at 1 micrograms/mL ADR. Although statistically non-significant, a trend towards the most prominent egect of 91.6% cell validity was noticed after 15 minutes of treatment. Our findings demonstrate that an improvement of ADR penetration into malignant cells can be obtained in this bioultrasound model. Wowever, caution is advised when extrapolating in vitro experimental results to in vivo phenomena.
Department of Obstetrics and Gynecology, Assaf Harofe Medical Center, Zerifin, Israel.
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Z
Weinraub
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Maymon
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Maymon
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M
Holtzinger
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Bukovsky
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Leibovici
D002463:Cell Membrane Permeability; D002470:Cell Survival / Q000187:drug effects; D018282:Cystadenocarcinoma, Mucinous / Q000378:metabolism* / Q000473:pathology; D004317:Doxorubicin / Q000493:pharmacokinetics*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D013010:Sonication; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014465:Ultrasonics; D018608:Ultrasonography, Doppler / Q000295:instrumentation
D004317:Doxorubicin