Effect of myosin B on guinea pig muscles--light microscopic and immunological aspects.
Experimental myositis was induced in guinea pigs by multiple inoculations with Myosin B fraction of the rabbit skeletal muscle. Quantitative histopathological features were studied and they include necrosis, central nucleation, inflammatory cellular reaction. Besides myositis, features of myocarditis were evident in 40% animals. Circulating immune complexes (CICs) in the sera of experimental animals were isolated by a polyethylene glycol (PEG) precipitation method. Presence of antimyosin B antibody in the immune complexes was characterised by an enzyme-linked immunosorbent assay. The role of circulating immune complexes in pathogenesis of experimental myositis and human polymyositis have been emphasised.
Krishnan
V V
VV
Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
Saraswathy
A
A
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D006168:Guinea Pigs; D007114:Immunization; D008297:Male; D008853:Microscopy / Q000379:methods; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D009218:Myosins / Q000494:pharmacology*; D009220:Myositis / Q000276:immunology*
D000936:Antigen-Antibody Complex; D009218:Myosins