Time course of formation of inositol phosphates during enzymatic hydrolysis of phytic acid (myo-inositol hexaphosphoric acid) by phytase determined by capillary isotachophoresis.
Capillary isotachophoresis with conductivity detection was applied to the investigation of the hydrolytic decomposition of phytic acid (myo-inositol hexaphosphoric acid) by phytase, and for the formation of the reaction products as a function of time. The quantitation of all analytes (besides phytic acid the mono- to penta-phosphorylated inositols and orthophosphate) can be carried out using two different buffer systems.
Institute of Analytical Chemistry, University of Vienna, Austria.
P
P
Blatný
F
F
Kvasnicka
E
E
Kenndler
D010832:6-Phytase / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods*; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000138:chemical synthesis*; D007700:Kinetics; D010766:Phosphorylation; D010833:Phytic Acid / Q000737:chemistry*
D007295:Inositol Phosphates; D010833:Phytic Acid; D010832:6-Phytase