[Effects of calcimycin on platelet adhesion to cultured bovine cerebral microvascular endothelial cells and its antagonism by drugs].
Calcimycin induced rabbit platelet adhesion to cultured bovine cerebral microvascular endothelial cells (CMEC) and its inhibition by triazelodiazepine (WEB 2086), 1,5-bis-(3,4-dimethoxyphenyl)-tetrahydro-(4H)-pyran (DMPP) and tetrandrine (Tet) were investigated. The results showed that calcimycin significantly increased platelet adhesion to CMEC. The platelet adhesion to CMEC was increased by 17.1% vs control after stimulation with calcimycin 0.01 mumol.L-1 for 25 min. WEB 2086 0.1, 1.0 and 10.0 mumol.L-1 or DMPP 0.1, 1.0 and 10.0 mumol.L-1 or Tet 0.1, 1.0 and 10.0 mumol.L-1 inhibited the calcimycin induced platelet adhesion to CMEC by 9.0, 22.9 and 23.1% or 9.7, 15.6 and 22.1% or 7.8, 15.6 and 24.6%, respectively. This indicates that DMPP and Tet may have perspectives in the prevention and treatment of cerebral vascular diseases.
Hu
J H
JH
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai.
Sun
D X
DX
Zeng
G Q
GQ
Lin
A Y
AY
Rui
Y C
YC
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D044182:Benzylisoquinolines; D001921:Brain / Q000098:blood supply*; D000001:Calcimycin / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D008297:Male; D008833:Microcirculation / Q000166:cytology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011714:Pyrans / Q000494:pharmacology; D011817:Rabbits; D014230:Triazoles / Q000494:pharmacology
D000470:Alkaloids; D001381:Azepines; D044182:Benzylisoquinolines; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D011714:Pyrans; D014230:Triazoles; C052518:WEB 2086; C079963:1,5-bis(3,4-dimethoxyphenyl)tetrahydropyran; C009438:tetrandrine; D000001:Calcimycin