Two novel HMW MAP2 variants with four microtubule-binding repeats and different projection domains.
The brain microtubule-associated protein MAP2 is composed of two high molecular (MAP2a and b) and one low molecular (MAP2c) weight isoforms. All these forms were thought to contain three repeated microtubule-binding domains in their C-terminal region but a MAP2c variant containing four repeats has recently been identified. We report here the existence of two high molecular weight MAP2 isoforms with four microtubule-binding domains in the sensory neuronal cell line ND 7/23. A stretch of 135 bp is missing in one of these forms suggesting that several HMW MAP2 variants can be produced by alternative splicing.
Unité INSERUM 282 affiliée au CNRS, Hôpital Henri Mondor, Créteil, France.
A
A
Langkopf
J
J
Guilleminot
J
J
Nunez
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation; D002459:Cell Fusion; D014644:Genetic Variation; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000235:genetics; D008870:Microtubules / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009447:Neuroblastoma; D009474:Neurons / Q000737:chemistry; D016133:Polymerase Chain Reaction; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured
D008869:Microtubule-Associated Proteins