Influence of six mutations of the protein C gene on the Gla domain conformation and calcium affinity.
The protein C Gla domain was studied in six families presenting a type II hereditary deficiency characterized by low activity in a coagulation assay and normal activity in an amidolytic assay. Five of these mutations, previously described by our group, affected Arg-5, Arg-1, Arg 229 and Ser 252. We report here the first natural Glu 7 to Asp mutation in a sixth family. We evaluated the binding of the mutated protein C to H11, a monoclonal antibody (mAb) known to recognize the sequence Phe4 to Arg9 of the Gla domain; the presence of calcium ions suppresses the recognition of this epitope by H11. Mutation of Arg229 to Gln and Ser252 to Asn did not modify the inhibition of protein C binding, whereas the Arg-1 to His mutation resulted in a loss of inhibition in the presence of CaCl2. This suggests that the protein C of this patient shows impaired carboxylation. The protein C from patients bearing the mutations Arg-5 to Trp, Arg-1 to Cys and Glu 7 to Asp bound poorly to H11 mAb, even in the absence of calcium ions. The calcium affinity of the Gla domain was studied by pseudo-affinity chromatography, in which protein C was successively eluted from a Mono Q column by CaCl2 10 mM and NaCl 0.6 M. Protein C from the patient bearing the Arg-5 to Asp mutation had a normal elution profile, suggesting that a modification of the propeptide cleavage site impairs the conformation of the Gla domain but not carboxylation.(ABSTRACT TRUNCATED AT 250 WORDS)
Gaussem
P
P
Groupe de Recherche sur la Thrombose, INSERM CJF 91-01, UFR des Sciences Pharmaceutiques et Biologiques, Université Paris V, France.
Gandrille
S
S
Duchemin
J
J
Emmerich
J
J
Alhenc-Gelas
M
M
Aillaud
M F
MF
Aiach
M
M
D015055:1-Carboxyglutamic Acid / Q000235:genetics; D000328:Adult; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001120:Arginine / Q000235:genetics; D001224:Aspartic Acid / Q000235:genetics; D002118:Calcium / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D003545:Cysteine / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006639:Histidine / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D011486:Protein C / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary
D000911:Antibodies, Monoclonal; D011486:Protein C; D001224:Aspartic Acid; D006639:Histidine; D015055:1-Carboxyglutamic Acid; D001120:Arginine; D003545:Cysteine; D002118:Calcium