A mouse cDNA encoding a protein with zinc-fingers and a KRAB domain shows similarity to human profilaggrin.
As part of our efforts to understand transcriptional regulation in the vertebrate eye lens, we have isolated a clone that encodes a zinc finger (Zf) protein from a newborn mouse lens cDNA library. Corresponding message for this protein is detectable in the lens, liver, heart, kidney, spleen and brain of newborn mice. A longer cDNA containing the complete ORF for the same protein was isolated from an adult mouse testis library. A conceptual translation of the testis cDNA sequence produces a 555-amino-acid (aa) protein that contains nine C-terminal Zf and an N-terminal domain found in a subset of C2H2 Zf, the Krüppel-associated box (KRAB). The aa sequence located between the KRAB domain and the Zf shows an unexpected similarity to human profilaggrin, a protein expressed in differentiating epidermal cells. Sequences that hybridize to this cDNA are detectable in ten other mammalian species.
Brady
J P
JP
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Piatigorsky
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D015139:Blotting, Southern; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D000091344:Filaggrin Proteins; D015723:Gene Library; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics*; D007668:Kidney / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors; D007908:Lens, Crystalline / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008322:Mammals; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D016366:Open Reading Frames; D009928:Organ Specificity; D010750:Phosphoproteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D051741:Kruppel-Like Transcription Factors; D010750:Phosphoproteins; D011498:Protein Precursors; D012097:Repressor Proteins; D014157:Transcription Factors