The effects of atrial natriuretic peptide on brain edema, intracranial pressure and cerebral energy metabolism in rat congenital hydrocephalus.
Atrial natriuretic peptide (ANP) regulates fluid and electrolyte homeostasis in the central nervous system. In this study, we evaluated the effects of ANP on brain edema, intracranial pressure (ICP) and cerebral energy metabolism in congenital hydrocephalus in rats. Brain edema, indicated by the longitudinal relaxation time (T1), was evaluated by 1H-magnetic resonance imaging (MRI). The ICP was monitored with a miniature pressure-transducer with telemetric system. Cerebral energy metabolism, indicated by PCr/Pi ratio, was measured by 31P-magnetic resonance spectroscopy (MRS). The rats were given 10 microliters of ANP in the left cerebral ventricle. Three different concentrations of ANP were given; 0.2 (group I), 2.0 (group II) and 20.0 (group III) micrograms/10 microliters, respectively. 10 microliters of saline was injected into the ventricle of the control group rats. There were no significant changes of ICP, T1 value and PCr/Pi ratio among the control group, group I and group II. In group III, in contrast, ICP decreased significantly at 20 minutes after ANP administration and stayed at this ICP level for 60 minutes. The T1 value decreased and PCr/Pi ratio increased 30 minutes after ANP administration. This study revealed that intraventricularly administered ANP could decrease ICP, reduce brain edema and improve the cerebral energy metabolism in rats with congenital hydrocephalus.
Minamikawa
J
J
Department of Neurosurgery, Kyoto University, Osaka, Japan.
Kikuchi
H
H
Ishikawa
M
M
Yamamura
K
K
Kanashiro
M
M
D000818:Animals; D000831:Animals, Newborn; D009320:Atrial Natriuretic Factor / Q000502:physiology*; D001921:Brain / Q000503:physiopathology; D001929:Brain Edema / Q000503:physiopathology*; D002552:Cerebral Ventricles / Q000503:physiopathology; D004734:Energy Metabolism / Q000502:physiology*; D006849:Hydrocephalus / Q000235:genetics / Q000503:physiopathology*; D007427:Intracranial Pressure / Q000502:physiology*; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014882:Water-Electrolyte Balance / Q000502:physiology*
D010710:Phosphates; D010725:Phosphocreatine; D009320:Atrial Natriuretic Factor