Modification of the electrophoretic mobility of neutral and charged polysaccharides.
Suitable buffer additives can either induce or reduce the electrophoretic mobility of certain polysaccharides during capillary electrophoresis. With water-soluble cellulose derivatives, adsorption of detergents on the analyte molecules governs the migration rates. Different types of adsorption sites, including multilayer formation, were found to be present on the cellulose-derived polymers. For highly charged polysaccharides (heparins), the electrophoretic mobility can be controlled by the use of ion-pairing reagents. The concentration and number of cationic charges appear useful in optimizing the separation selectivity. Migration models based on secondary thermodynamic equilibria are presented and evaluated for different polysaccharides.
Stefansson
M
M
Department of Chemistry, Indiana University, Bloomington 47405.
Novotny
M
M
D002240:Carbohydrate Sequence; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004586:Electrophoresis / Q000379:methods; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry*
D011134:Polysaccharides; D002482:Cellulose