Expression of 5-lipoxygenase in lymphoblastoid B and T cells.
The expression of arachidonate 5-lipoxygenase (5-LOX, arachidonate: oxygen 5-oxidoreductase; EC 1.13.11.34) and the 5-lipoxygenase activating protein (FLAP) genes in lymphoblastoid B and T cell lines was studied at the transcriptional level by reverse transcription-PCR analysis. Two B cell lines, CESS and SKW 6.4, were found to express both 5-LOX and FLAP mRNA. One T cell line, MOLT 4, expressed only FLAP mRNA. Upon stimulation of intact cells by calcium ionophore, B lymphoblastoid cells produced 5, 12 and 15 hydroxyeicosatetraenoic acids as well as leukotriene B4.
el Makhour-Hojeij
Y
Y
U.R.A. CNRS 1485, Faculté de Médecine, Limoges, France.
Baclet
M C
MC
Chable-Rabinovitch
H
H
Beneytout
J L
JL
Cook
J
J
D058927:5-Lipoxygenase-Activating Proteins; D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis* / Q000235:genetics; D016718:Arachidonic Acid / Q000378:metabolism; D001402:B-Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D014158:Transcription, Genetic
D058927:5-Lipoxygenase-Activating Proteins; C548592:ALOX5AP protein, human; D002352:Carrier Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D016718:Arachidonic Acid; D001094:Arachidonate 5-Lipoxygenase