Versatile transformation vectors to assay the promoter activity of DNA elements in plants.
A convenient vector system was developed to evaluate transcriptional promoter activities in plants. Two primary vectors, optionally containing the cauliflower mosaic virus (CaMV) 35S -47 or -90 minimal promoters, offer multiple sites for cloning the sequence of interest upstream from the beta-glucuronidase gene (gusA). The promoter-gusA cassette can be transferred to a binary vector containing the selectable neomycin phosphotransferase II-encoding gene (nptII) next to the left border. In addition, the transferred DNA (T-DNA) contains the chloramphenicol acetyltransferase gene (cat) driven by the CaMV 35S promoter. Activity of cat can serve as a reference for gusA expression to correct for effect of chromosomal position or T-DNA copy number.
Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, The Netherlands.
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Memelink
D001483:Base Sequence; D017796:Caulimovirus / Q000235:genetics; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D003001:Cloning, Molecular / Q000379:methods; D018744:DNA, Plant / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D005966:Glucuronidase / Q000096:biosynthesis; D019868:Kanamycin Kinase; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis; D010944:Plants / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014162:Transfection / Q000379:methods*; D014170:Transformation, Genetic
D018744:DNA, Plant; D015500:Chloramphenicol O-Acetyltransferase; D017853:Phosphotransferases (Alcohol Group Acceptor); D019868:Kanamycin Kinase; D005966:Glucuronidase