Antiviral activity of primate gamma delta T lymphocytes isolated by magnetic cell sorting.
The exposure of human or rhesus monkey peripheral blood mononuclear cells (PBMCs) to interleukin 2 (IL-2) in vitro resulted in a selective outgrowth of gamma delta lymphocytes. Using positive selection by monoclonal antibodies and magnetic beads, gamma delta T lymphocytes were isolated from these cultures. Without priming by viral antigens, the purified gamma delta T lymphocytes lyse immunodeficiency virus-infected cells substantially better than the uninfected counterparts.
Wallace
M
M
Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison 53706.
Gan
Y H
YH
Pauza
C D
CD
Malkovsky
M
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006678:HIV / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D018189:Immunomagnetic Separation; D007376:Interleukin-2 / Q000494:pharmacology*; D008253:Macaca mulatta; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D012016:Reference Values; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D016692:Receptors, Antigen, T-Cell, gamma-delta