Sera from some patients with antibody-associated paraneoplastic encephalomyelitis/sensory neuronopathy recognize the Ro-52K antigen.
Screening a small cell lung cancer cDNA library with serum from a patient with antibody-associated paraneoplastic sensory neuronopathy (Anti-Hu syndrome) resulted in the isolation of a cDNA clone encoding the Ro-52 kD antigen. The Ro-52 kD antigen is one of the major antigens recognized by the sera of patients with Sjögren's syndrome and systemic lupus erythematosus. Further investigation revealed that the sera of only a small percentage (4%) of patients with the Hu syndrome reacted with Ro-52 kD antigen. The cross-reactivity may result from a domain of high homology between Ro-52 kD and HuD antigen. These results emphasize the necessity of using recombinant antigens whenever possible when assaying for assaying for specific antibodies.
Laboratory of Molecular Neuro-Oncology, Memorial Sloan-Kettering Cancer Center, N.Y., N.Y.
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Manley
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Wong
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Dalmau
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Elkon
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Posner
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Furneaux
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology*; D001324:Autoantigens / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D001769:Blood / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; D004679:Encephalomyelitis / Q000097:blood*; D006801:Humans; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009419:Nerve Tissue Proteins; D010257:Paraneoplastic Syndromes / Q000097:blood*; D020733:RNA, Small Cytoplasmic; D016601:RNA-Binding Proteins / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012261:Ribonucleoproteins / Q000235:genetics / Q000276:immunology*; D012678:Sensation Disorders / Q000097:blood*; D000099207:SS-A Antigen
D000906:Antibodies; D001324:Autoantigens; D018076:DNA, Complementary; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; D015335:Molecular Probes; D009419:Nerve Tissue Proteins; D020733:RNA, Small Cytoplasmic; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C498010:ELAVL4 protein, human; C116006:RO60 protein, human