Improved separation of radioactively labelled cellular phospholipids by high-performance liquid chromatography.
An improved high-performance liquid chromatographic method for the separation and determination of radioactively labelled cellular phospholipids is described. The method is based on separation of phospholipids on a 250 x 4 mm I.D. LiChrospher DIOL 100 (5 microns) column, fitted with a 50 x 4 mm I.D. LiChrospher Si 60 (5 microns) precolumn and a gradient of 5% H3PO4 and acetonitrile. It allows the determination of small amounts of labelled phosphatidylcholine and sphingomyelin due to the sharp elution profile in spite of long retention times.
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany.
B
B
Trümbach
G
G
Rogler
K J
KJ
Lackner
G
G
Schmitz
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling; D010713:Phosphatidylcholines / Q000302:isolation & purification; D010743:Phospholipids / Q000302:isolation & purification*; D012867:Skin / Q000737:chemistry / Q000166:cytology; D013056:Spectrophotometry, Ultraviolet; D013109:Sphingomyelins / Q000302:isolation & purification; D013631:Tangier Disease / Q000378:metabolism
D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins