Activation of neutrophils by a chemically separated but optically coupled neutrophil population undergoing respiratory burst.
Neutrophils from pig blood were used as a model system to investigate the optical communication between cells. It was found that neutrophils stimulated to undergo respiratory burst can activate a second, chemically separated, but optically coupled population of neutrophils. The response of the latter was visualized as a temporary rising of their low-level chemiluminescence and an enhanced generation of superoxide radicals detected by both the reduction of ferricytochrome c and spin trapping. The results provide evidence that a long-range optical coupling of biological significance between living cells exists.
Institute of Biophysics, Chinese Academy of Sciences, Chaoyong District, Beijing.
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D000818:Animals; D003497:Cyclic N-Oxides; D003574:Cytochrome c Group / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D008163:Luminescent Measurements; D018375:Neutrophil Activation / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010084:Oxidation-Reduction; D016897:Respiratory Burst / Q000502:physiology*; D013113:Spin Labels; D013481:Superoxides / Q000378:metabolism; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003497:Cyclic N-Oxides; D003574:Cytochrome c Group; D005609:Free Radicals; D013113:Spin Labels; D013481:Superoxides; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D013755:Tetradecanoylphorbol Acetate