Modulations of 92kDa gelatinase B and its inhibitors are associated with HIV-1 infection in human macrophage cultures.
The macrophage-secreted 92-kDa type IV collagenase and metalloproteinases play a critical role in cell microenvironment regulation and cell movement. HIV infection of macrophages might be capable of deregulating the expression of these gelatinases. Hence, human monocyte-derived-macrophages were infected by lymphotropic HIV-1/Lai and monocytropic HIV-1/DAS isolates. Gelatinase activity and gelatinase and inhibitor (TIMP, alpha 2M) biosyntheses were evaluated in supernatants and cellular extracts. Our data suggest that HIV infection facilitates gelatinase secretion and intracellular inhibitor retention. These argue for the increase of free proteinase that could degrade barriers, which would permit cell movement and viral dissemination into tissues.
Chapel
C
C
Laboratoire de Neuropathologie expérimentale et Neurovirologie, CEA/DSV/DPTE/SSA, Fontenay aux Roses, France.
Camara
V
V
Clayette
P
P
Salvat
S
S
Mabondzo
A
A
Leblond
V
V
Marcé
D
D
Lafuma
C
C
Dormont
D
D
D002478:Cells, Cultured; D017364:Collagenases / Q000096:biosynthesis*; D005780:Gelatin / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis*; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism* / Q000821:virology; D020780:Matrix Metalloproteinase 9; D061965:Matrix Metalloproteinase Inhibitors; D008970:Molecular Weight; D009000:Monocytes; D013462:Sulfur Radioisotopes; D013997:Time Factors; D019714:Tissue Inhibitor of Metalloproteinases
D006023:Glycoproteins; D016655:HIV Core Protein p24; D061965:Matrix Metalloproteinase Inhibitors; D013462:Sulfur Radioisotopes; D019714:Tissue Inhibitor of Metalloproteinases; D005780:Gelatin; D017364:Collagenases; D020780:Matrix Metalloproteinase 9