Genotyping of alcohol dehydrogenase at the ADH2 and ADH3 loci by using a polymerase chain reaction and restriction-fragment-length polymorphism in Chinese alcoholic cirrhotics and non-alcoholics.
We investigated the genotype of ADH2 and ADH3 in Chinese alcoholic cirrhotics and non-alcoholics by using a polymerase chain reaction and restriction-fragment-length polymorphism. This method is non-radioactive, easy to implement with good reproducibility. In the Chinese population, the frequencies of the ADH2*1 and ADH3*2 alleles were significantly higher in the alcoholic cirrhotic patients (53%; 23%) than in the viral hepatitis cirrhotics (32%; 8%) and the gastric and/or duodenal ulcer control patients (25%; 6%). On the other hand, the gastric and/or duodenal ulcer control patients and the viral hepatitis cirrhotic patients showed similar allele frequencies for the polymorphic ADH2 and ADH3 genes. These findings suggest that the alleles ADH2*2 and ADH3*1, coding for the high-Vmax beta 2-ADH and gamma 1-ADH, respectively, may play a protective role against alcoholism in Chinese patients.
Chao
Y C
YC
Department of Internal Medicine, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Wang
M F
MF
Tang
H S
HS
Hsu
C T
CT
Yin
S J
SJ
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D002681:China; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006525:Hepatitis, Viral, Human / Q000150:complications; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008103:Liver Cirrhosis / Q000201:enzymology / Q000209:etiology / Q000235:genetics; D008104:Liver Cirrhosis, Alcoholic / Q000201:enzymology / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010437:Peptic Ulcer / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D007527:Isoenzymes; D000426:Alcohol Dehydrogenase