Cloning of a murine IL-11 receptor alpha-chain; requirement for gp130 for high affinity binding and signal transduction.
An adult mouse liver cDNA library was screened with oligonucleotides corresponding to the conserved WSXWS motif of the haemopoietin receptor family. Using this method, cDNA clones encoding a novel receptor were isolated. The new receptor, named NR1, was most similar in sequence and predicted structure to the alpha-chain of the IL-6 receptor and mRNA was expressed in the 3T3-L1 pre-adipocytic cell line and in a range of primary tissues. Expression of NR1 in the factor-dependent haemopoietic cell line Ba/F3 resulted in the generation of low affinity receptors for IL-11 (Kd approximately 10 nM). The capacity to bind IL-11 with high affinity (Kd = 300-800 pM) appeared to require coexpression of both NR1 and gp130, the common subunit of the IL-6, leukaemia inhibitory factor (LIF), oncostatin M (OSM) and ciliary neurotrophic factor (CNTF) receptors. The expression of both NR1 and gp130 was also necessary for Ba/F3 cells to proliferate and M1 cells to undergo macrophage differentiation in response to IL-11.
Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria.
D J
DJ
Hilton
A A
AA
Hilton
A
A
Raicevic
S
S
Rakar
M
M
Harrison-Smith
N M
NM
Gough
C G
CG
Begley
D
D
Metcalf
N A
NA
Nicola
T A
TA
Willson
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D050822:Cytokine Receptor gp130; D018076:DNA, Complementary; D053703:Interleukin-11 Receptor alpha Subunit; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D018123:Receptors, Interleukin / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D053702:Receptors, Interleukin-11; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
D015703:Antigens, CD; D018076:DNA, Complementary; C508429:Il11ra1 protein, mouse; C508431:Il11ra2 protein, mouse; C494200:Il6st protein, mouse; D053703:Interleukin-11 Receptor alpha Subunit; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11; D050822:Cytokine Receptor gp130