Repeated sequences and the sites of genome rearrangements in bacteriophages of Lactobacillus delbrueckii subsp. lactis.
We have sequenced the KIS-element, a 1.5 kb insertion segment present in the genome of Lactobacillus delbrueckii subsp. lactis phage LL-K, but absent from its close relative, phage LL-H. The KIS-element showed some sequence features of a transposable element: it was flanked by direct repeats of a 20 nt long sequence which was in the genome of LL-H as a target sequence. The KIS-element contained two putative ORFs. The C-terminal part of ORF333 consisted of clusters of direct repeats, capable of coding Lys/Arg-Gly-Asp motifs, which are known to be able to bind to glycoproteins. A homologous counterpart of the KIS-element was also found in the genome of prolate-headed L. delbrueckii subsp. lactis phage JCL1032, even though the phage JCL1032 is not a close relative of phage LL-K. The nucleotide sequence comparison between KIS-element and its homologous counterpart in JCL1032 showed that there have occurred several genome rearrangements at the repeat clusters.
Department of Genetics, University of Oulu, Finland.
P
P
Forsman
T
T
Alatossava
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015321:Gene Rearrangement; D016679:Genome, Viral; D007778:Lactobacillus / Q000235:genetics / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004269:DNA, Bacterial; D004279:DNA, Viral