Experimental pancreatic cancer in the rat treated by photodynamic therapy.
Selective histological necrosis of experimental pancreatic carcinoma by photodynamic therapy (PDT) has been successful with haematoporphyrin derivatives and phthalocyanine as photosensitizers. This report describes the feasibility of PDT with pheophorbide A as the photosensitizer to treat azaserine-induced pancreatic rat carcinoma and analyses survival of the animals. An organ distribution study 24 h after pheophorbide A administration (9 mg/kg intravenously) gave a selectivity ratio of 13.5:1 between tumour and surrounding tissue. Light of 660 nm and 100 J/cm2 induced selective necrosis of the tumour. Six of nine rats were cured in 120 days whereas all 36 control animals died within 35 days (P < 0.01). The pancrease and hepatic pedicle were relatively unaffected by PDT, but the duodenum was injured.
Evrard
S
S
Department of Surgery A, Hôpitaux Universitaires, Strasbourg, France.
Keller
P
P
Hajri
A
A
Balboni
G
G
Mendoza-Burgos
L
L
Damgé
C
C
Marescaux
J
J
Aprahamian
M
M
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D002734:Chlorophyll / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000627:therapeutic use; D004386:Duodenum / Q000473:pathology; D009336:Necrosis; D010179:Pancreas / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010778:Photochemotherapy / Q000009:adverse effects / Q000379:methods*; D011838:Radiation-Sensitizing Agents / Q000009:adverse effects / Q000627:therapeutic use*; D051381:Rats; D011917:Rats, Inbred Lew; D016019:Survival Analysis; D013997:Time Factors
D011838:Radiation-Sensitizing Agents; D002734:Chlorophyll; C032623:pheophorbide a