GD2 ganglioside-specific monoclonal antibody reacts with murine cytotoxic T lymphocytes reactive with FBL-3N erythroleukaemia.
Ganglioside expression on cytotoxic T lymphocytes induced against the mouse erythroleukaemia line FBL-3N, was analysed, compared with that of naive T lymphocytes in the thymus, lymph nodes and spleen. Although normal uncultured and cultured T lymphocytes expressed no GD2, GD3 or GM2 gangliosides, cytotoxic T cells with CD4+CD8-, CD4-CD8+, and CD4-CD8- phenotypes reacted with anti-GD2 monoclonal antibody with various intensities. The reactivity with anti-GD2 was associated with the intensity of cytotoxic activity as analysed using cytotoxic T lymphocyte (CTL) clones established from the bulk CTLs of each phenotype. An increase of the mRNA expression of GM2/GD2 synthase gene was demonstrated by Northern blot hybridization using RNA extracted from thymocytes, spleen cells, Con A-treated spleen cells and various types of CTL cells. These results indicated that GD2 ganglioside expression might be associated with the functional differentiation of murine T lymphocytes.
Department of Periodontology, School of Dentistry, Nagasaki University, Japan.
A
A
Yoshimura
K
K
Takamiya
I
I
Kato
E
E
Nakayama
H
H
Shiku
K
K
Furukawa
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015152:Blotting, Northern; D003208:Concanavalin A / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005434:Flow Cytometry; D005678:G(M2) Ganglioside / Q000096:biosynthesis; D005732:Gangliosides / Q000096:biosynthesis* / Q000276:immunology*; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology; D015854:Up-Regulation; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D000911:Antibodies, Monoclonal; D003208:Concanavalin A; D005678:G(M2) Ganglioside; D005732:Gangliosides; D017350:N-Acetylgalactosaminyltransferases; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C019403:ganglioside, GD2; C023220:(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase