Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging.
The tomato Cf-9 gene confers resistance to infection by races of the fungus Cladosporium fulvum that carry the avirulence gene Avr9. The Cf-9 gene was isolated by transposon tagging with the maize transposable element Dissociation. The DNA sequence of Cf-9 encodes a putative membrane-anchored extracytoplasmic glycoprotein. The predicted protein shows homology to the receptor domain of several receptor-like protein kinases in Arabidopsis, to antifungal polygalacturonase-inhibiting proteins in plants, and to other members of the leucine-rich repeat family of proteins. This structure is consistent with that of a receptor that could bind Avr9 peptide and activate plant defense.
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
D A
DA
Jones
C M
CM
Thomas
K E
KE
Hammond-Kosack
P J
PJ
Balint-Kurti
J D
JD
Jones
D000595:Amino Acid Sequence; D002963:Cladosporium / Q000235:genetics / Q000502:physiology*; D016384:Consensus Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D005656:Fungal Proteins / Q000235:genetics; D018390:Gene Targeting; D017343:Genes, Plant; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006031:Glycosylation; D018551:Solanum lycopersicum / Q000737:chemistry / Q000235:genetics* / Q000382:microbiology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D017931:DNA Primers; D004251:DNA Transposable Elements; D005656:Fungal Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D010940:Plant Proteins; C073605:AVR9 protein, Cladosporium fulvum