Mapping, cloning and sequencing of a glycoprotein-encoding gene from bovine herpesvirus type 1 homologous to the gE gene from HSV-1.
In order to map and identify the glycoprotein-encoding gene from bovine herpesvirus type 1 (BHV-1), homologous to the gE glycoprotein from herpes simplex virus type 1 (HSV-1), a region of the unique short sequence from the BHV-1 genome has been sequenced. The sequenced region contains an ORF coding for a polypeptide of 575 amino acids (aa). The aa sequence presents substantial similarity to that of the glycoprotein gE from HSV-1 and to homologous proteins of related viruses such as pseudorabies virus, equine herpesvirus type 1 and varicella zoster virus. The aa sequence presents additional characteristics compatible with the structure of a viral glycoprotein: signal peptide, putative glycosylation sites and a long C-terminal transmembrane alpha-helix.
Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Bellaterra, Spain.
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Rebordosa
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Piñol
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Pérez-Pons
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Lloberas
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Naval
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Querol
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular / Q000379:methods; D016384:Consensus Sequence; D005814:Genes, Viral; D006031:Glycosylation; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D004861:Herpesvirus 1, Equid / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics*; D011558:Herpesvirus 1, Suid / Q000235:genetics; D014645:Herpesvirus 3, Human / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D014764:Viral Proteins
D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins; C044768:glycoprotein E, herpes simplex virus type 1