Protein C is responsible for the rapid inactivation of factor Va following blood clotting in vitro.
When whole blood is allowed to clot in vitro, factor V is rapidly activated to factor Va which is subsequently inactivated. We developed two monoclonal anti-protein C antibodies, one of which inhibits protein C activation and the other inhibits protein C activity. The addition of either antibody to blood before clotting in vitro significantly inhibited the inactivation of factor Va, confirming the essential role of protein C in mediating the rapid inactivation of factor Va.
Jackson
D E
DE
Department of Medicine, Monash University, Box Hill Hospital, Victoria, Australia.
Mitchell
C A
CA
Salem
H H
HH
D000911:Antibodies, Monoclonal; D015943:Factor Va / Q000037:antagonists & inhibitors*; D006801:Humans; D066298:In Vitro Techniques; D011486:Protein C / Q000276:immunology / Q000502:physiology*; D013927:Thrombosis / Q000503:physiopathology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D011486:Protein C; D015943:Factor Va