[Restriction digestion of two kind of baculoviruses and the location of their p10 genes].
The length of SeNPV and LsNPV genomes DNA were calculated to be 133kb and 164kb respectively, according to digestion with 5-8 restriction endonuleases. In order to map p10 gene in Baculoviruses DNA by pure probe, two probe vectors, pAcHP106 and pAcEP102, were constructed with 3'-0. 18kb and 3'-0. 18kb + 5'-0.24kb (coding sequence and promoter) from AcNPV p10 gene. The p10 genes of both baculoviruses, SeNPV and LsNPV, are located with 0.18kb and 0.42kb probes produced by random primer labeling.
Department of Virology, Wuhan University.
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Y
Huang
Y
Y
Qi
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Li
T
T
Jin
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology; D017385:Sequence Homology; D015678:Viral Structural Proteins / Q000235:genetics*
D015342:DNA Probes; D004279:DNA, Viral; D015678:Viral Structural Proteins