The influence of in vivo stimulation on functional activity of rat macrophages.
The objective of this study was to investigate the effects of intraperitoneal administration of thioglycollate or LPS and subcutaneous injection of turpentine on chemotaxis, phagocytosis and respiratory burst of rat alveolar (AM) and peritoneal (PM) macrophages. All the stimulants caused an increase in chemotaxis of PM and its decrease in AM. Phagocytosis of AM and PM was increased after thioglycollate injection, LPS caused an increase in phagocytotic activity of AM and its decrease in PM; turpentine had no effect. Turpentine and thioglycollate caused a decrease and LPS an increase in respiratory burst activity of AM; all types of treatment caused an increase in this activity in PM.
Jan Zurzycki Institute of Molecular Biology, Department of Animal Biochemistry, Jagiellonian University, Kraków, Poland.
B
B
Turyna
M
M
Boś
J
J
Jurek
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000494:pharmacology; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000502:physiology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000502:physiology; D008297:Male; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D016897:Respiratory Burst / Q000187:drug effects; D013268:Stimulation, Chemical; D013864:Thioglycolates / Q000494:pharmacology; D014425:Turpentine / Q000494:pharmacology
D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D013864:Thioglycolates; C017487:2-mercaptoacetate; D014425:Turpentine