Aromatic DNA adducts in larynx biopsies and leukocytes.
Larynx cancer is strongly associated with tobacco smoking. The objective of this work was an analysis of aromatic DNA adducts in tumour and non-tumour larynx cells by means of the 32P-postlabelling method. Peripheral blood leukocytes were used as a reference tissue. The presence of aromatic DNA adducts was demonstrated in all the studied tissues obtained after surgery of larynx tumours. The highest level of DNA adducts was found in larynx tumour cells, followed by non-tumour larynx cells, which exceeded that found in leukocytes almost 2.5 times. Large interindividual differences were detected between subjects. The adduct level in tumour/non-tumour correlated only moderately. However a high correlation was found between the level of DNA adducts in larynx (tumour and non-tumour) cells and that in leukocytes.
Center for Nutrition and Toxicology, Karolinska Institute, Huddinge, Sweden.
K
K
Szyfter
K
K
Hemminki
W
W
Szyfter
Z
Z
Szmeja
J
J
Banaszewski
K
K
Yang
D000328:Adult; D000368:Aged; D001345:Autoradiography; D001706:Biopsy; D018736:DNA Adducts / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D007553:Isotope Labeling; D007822:Laryngeal Neoplasms / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010761:Phosphorus Radioisotopes; D010865:Pilot Projects; D011083:Polycyclic Compounds / Q000378:metabolism* / Q000633:toxicity; D012907:Smoking / Q000009:adverse effects / Q000097:blood / Q000378:metabolism
D018736:DNA Adducts; D010761:Phosphorus Radioisotopes; D011083:Polycyclic Compounds