[Chromatin proteins binding vitamin E].
It was discovered that alpha-tocopherol binding with isolated chromatin is specific only when fraction of tocopherol-binding proteins from a nuclear extract with 1% triton X-100 is present. During the chromatography of chromatin incubated with [H] alpha-tocopherol on hydroxyapatite a specific binding activity was present only in fraction eluted with 2M NaCl + 5M urea. Quantitative changes in the protein content of this fraction during a hypovitaminosis are found. It is shown that vitamin E can effect the RNA polymerase activity of a nuclear matrix in the in vitro assays. It is suggested that the presence of tocopherol-binding proteins of chromatin in necessary for such an action of alpha-tocopherol.
A A
AA
Kapralov
G V
GV
Donchenko
G V
GV
Petrova
D002843:Chromatin / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism*; D011485:Protein Binding; D014158:Transcription, Genetic; D014810:Vitamin E / Q000378:metabolism*
D002843:Chromatin; D009698:Nucleoproteins; D014810:Vitamin E; D012321:DNA-Directed RNA Polymerases
Issledovanie belkov khromatina, sviazyvaiushchikh vitamin E.