Determination of metal ions by capillary electrophoresis using on-column complexation with 4-(2-pyridylazo)resorcinol following trace enrichment by peak stacking.
Co2+, Fe2+, Cu2+ and Zn2+ were separated and detected following derivatization with 4-(2-pyridylazo)resorcinol by capillary electrophoresis with UV-Vis detection. Parameters which were examined include both on-column and pre-column complexation, limit of detection, capillary loadability, linear dynamic range and reproducibility. A sample stacking technique was investigated in order to obtain better detection limits and greater sensitivity. Detection limits of 1 x 10(-8) M were achieved for Co2+, Fe2+ and Zn2+ and 4 x 10(-7) M for Cu2+. Mass detection limits were 0.2 fmol for Co2+, Fe2+ and Zn2+ and 7.0 fmol for Cu2+. The use of this method for the determination of metals in vitamin tablets and a pond water sample is presented.
Center for Bioanalytical Research, University of Kansas, Lawrence 66047.
F B
FB
Regan
M P
MP
Meaney
S M
SM
Lunte
D002021:Buffers; D002412:Cations / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000302:isolation & purification*; D012118:Resorcinols / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000032:analysis
D002021:Buffers; D002412:Cations; D008670:Metals; D012118:Resorcinols; D014867:Water; C028398:4-(2-pyridylazo)resorcinol