Bacteriophages as tools for vaccine development.
The construction of live recombinant bacterial vaccines requires a reasonably sophisticated genetic system for the introduction, stabilization and expression of foreign antigen genes. Bacteriophages offer a rich collection of tools that can be used for vaccine construction, including site-specific integration-proficient vectors, non-antibiotic selectable markers and signals for efficient transcription and translation of foreign genes. We describe the characterization of a temperate phage of the mycobacteria, mycobacteriophage L5, and application of these phage studies for the construction of recombinant BCG vaccines.
Hatfull
G F
GF
Department of Biological Sciences, University of Pittsburgh, PA.
Barsom
L
L
Chang
L
L
Donnelly-Wu
M
M
Lee
M H
MH
Levin
M
M
Nesbit
C
C
Sarkis
G J
GJ
D001500:BCG Vaccine; D001435:Bacteriophages / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D016679:Genome, Viral; D008242:Lysogeny; D009163:Mycobacterium bovis / Q000821:virology; D015163:Superinfection; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic
D001500:BCG Vaccine; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic