Expression of Bcl-2 protein in murine neural cells in culture.
To explore the role of the protooncogene bcl-2 in the prevention of programmed cell death in the nervous system, we investigated its expression in mouse neural cells in primary culture. The 26 kDa protein product, Bcl-2, was detected by immunocytochemistry and immunoblotting in cultured neurons, astrocytes and oligodendrocytes, but the immunoreactivity of microglial cells was not detectable by immunoblotting. The subcellular distribution of Bcl-2 was similar between in vivo (brain) and in vitro (culture) and between cultured neurons and astrocytes, while the content was higher in astrocytes than in neurons. The substantial expression of bcl-2 in primary cultured brain cells suggests that it has some physiological control in the brain over programmed cell death, which may be exerted not only in neurons but also in some glial cells such as astrocytes.
Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, Kodaira, Japan.
M
M
Mizuguchi
K
K
Ikeda
M
M
Asada
S
S
Mizutani
S
S
Kamoshita
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000276:immunology; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D017628:Microglia / Q000166:cytology / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000276:immunology; D019253:Proto-Oncogene Proteins c-bcl-2; D013347:Subcellular Fractions / Q000378:metabolism
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D019204:GTP-Binding Proteins