The existence of a growth-specific DNA binding factor for the promoter region of mouse ST2 gene.
A comparison of the 5'-flanking regions of human and mouse ST2 genes revealed the presence of two highly conserved DNA sequences. The promoter activity assay with a luciferase gene as a reporter showed that the deletion of the upstream conserved region diminished the transcriptional activity in growing BALB/c-3T3 cells. By electrophoretic mobility-shift analysis, the presence of a factor that binds to the positive regulatory region of the mouse ST2 gene was found in growing but not in quiescent BALB/c-3T3 cells. These results suggest the functional importance of this conserved region and the requirement of a binding factor for the expression of the ST2 gene.
Department of Biology, Jichi Medical School, Tochigi, Japan.
S
S
Tominaga
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M
Kato-Yamazaki
K
K
Yanagisawa
K
K
Kawakami
T
T
Tetsuka
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002467:Cell Nucleus / Q000737:chemistry; D017124:Conserved Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis*; D006801:Humans; D000072179:Interleukin-1 Receptor-Like 1 Protein; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D011956:Receptors, Cell Surface; D018123:Receptors, Interleukin; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; C079241:IL1RL1 protein, human; C486189:Il1rl1 protein, mouse; D000072179:Interleukin-1 Receptor-Like 1 Protein; D008565:Membrane Proteins; D011506:Proteins; D011956:Receptors, Cell Surface; D018123:Receptors, Interleukin; D004247:DNA