Study of efficacies of leukocyte-depleted terminal blood cardioplegia in 24-hour preserved hearts.
To evaluate the effect of leukocyte-depleted terminal blood cardioplegia on prolonged preservation, 41 canine hearts were stored in modified Collins' solution and transplanted heterotopically. Hearts were transplanted soon after harvesting in group 1 and after 24-hour preservation in groups 2, 3, and 4. Blood cardioplegia was applied just before aortic unclamping in groups 3 and 4; group 3 received simple blood cardioplegia and group 4 received leukocyte-depleted cardioplegia. The percentage of the preload recruitable stroke work and diastolic compliance after transplantation compared with the preharvesting value in group 4 did not differ from those in group 1, but the percentage of the preload recruitable stroke work in groups 2 and 3 was significantly lower than that in groups 1 and 4. The percentage of diastolic compliance in groups 2 and 3 was significantly higher than that in groups 1 and 4. Coronary blood flow 40 minutes after aortic unclamping in group 4 did not differ from that in group 1, but was significantly higher than the blood flows in groups 2 and 3. Significant production of malondialdehyde was detected during terminal blood cardioplegia and 10 minutes after aortic unclamping in groups 2 and 3, but never in groups 1 and 4. After leukocyte-depleted terminal cardioplegia, the myocardial adenosine triphosphate content increased to the preharvesting value in group 4. Our results suggest that leukocyte-depleted terminal blood cardioplegia may be effective in replenishing the energy-depleted myocardium and reducing reperfusion injury, resulting in adequate cardiac function.
Fukushima
N
N
First Department of Surgery, Osaka University Medical School, Japan.
Shirakura
R
R
Nakata
S
S
Kaneko
M
M
Miyagawa
S
S
Naka
Y
Y
Chang
J C
JC
Matsumiya
G
G
Nakano
S
S
Matsuda
H
H
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002314:Cardioplegic Solutions; D004285:Dogs; D005260:Female; D016027:Heart Transplantation; D006982:Hypertonic Solutions; D007962:Leukocytes; D008297:Male; D009206:Myocardium / Q000737:chemistry; D009926:Organ Preservation / Q000379:methods*; D016042:Transplantation, Heterotopic
D002314:Cardioplegic Solutions; C024102:Collins' solution; D006982:Hypertonic Solutions; D000255:Adenosine Triphosphate