Multicycle infection of hepatitis C virus in cell culture and inhibition by alpha and beta interferons.
We previously reported that a clone of a human lymphocytic cell line, HPBMa clone 10-2, supports the genome replication of hepatitis C virus (HCV). In the present study, the multicycle transmission of HCV from infected cells to new cells was demonstrated by coculture with drug-resistant cells as recipients; i.e., the system was proved productive for infectious HCV. Inhibition of viral replication by alpha and beta interferons was observed.
Shimizu
Y K
YK
Department of Viral Diseases and Vaccine Control, National Institute of Health, Tokyo.
Yoshikura
H
H
D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells; D017931:DNA Primers; D004351:Drug Resistance; D005814:Genes, Viral; D016174:Hepacivirus / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D006921:Hygromycin B / Q000494:pharmacology; D016898:Interferon-alpha / Q000494:pharmacology*; D016899:Interferon-beta / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008214:Lymphocytes; D008969:Molecular Sequence Data; D009355:Neomycin / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis
D017931:DNA Primers; D016898:Interferon-alpha; D017361:Viral Nonstructural Proteins; D006921:Hygromycin B; D016899:Interferon-beta; D007371:Interferon-gamma; D009355:Neomycin