Mechanistic implications of cyclic ADP-ribose hydrolysis and methanolysis catalyzed by calf spleen NAD+glycohydrolase.
Calf spleen NAD+glycohydrolase, besides its well known reactions, was shown to catalyze hydrolysis and methanolysis (with retention of configuration) of cyclic ADP-ribose, indicating that this classical NADase also belongs to the class of cyclic ADP-ribose hydrolases. No formation of cyclic ADP-ribose could be detected during the hydrolysis of beta-NAD+; moreover, the kinetic parameters of cyclic ADP-ribose seem to rule out that it is a kinetically competent reaction intermediate in the conversion of NAD+ into ADP-ribose by this enzyme.
Laboratoire de Chimie Bioorganique (URA CNRS 1386), Faculté de Pharmacie, Strasbourg-Illkirch, France.
H
H
Muller-Steffner
M
M
Muzard
N
N
Oppenheimer
F
F
Schuber
D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002384:Catalysis; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D036563:Cyclic ADP-Ribose; D006868:Hydrolysis; D007700:Kinetics; D008697:Methane; D009243:NAD / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology*
D002250:Carbon Radioisotopes; D009243:NAD; D036563:Cyclic ADP-Ribose; D000246:Adenosine Diphosphate Ribose; D009244:NAD+ Nucleosidase; D008697:Methane