Herbicide-resistant tobacco plants expressing the fused enzyme between rat cytochrome P4501A1 (CYP1A1) and yeast NADPH-cytochrome P450 oxidoreductase.
Transgenic tobacco (Nicotiana tabacum cv Xanthi) plants expressing a genetically engineered fused enzyme between rat cytochrome P4501A1 (CYP1A1) and yeast NADPH-cytochrome P450 oxidoreductase were produced. The expression plasmid pGFC2 for the fused enzyme was constructed by insertion of the corresponding cDNA into the expression vector pNG01 under the control of the cauliflower mosaic virus 35S promoter and nopaline synthase gene terminator. The fused enzyme cDNA was integrated into tobacco genomes by Agrobacterium infection techniques. In transgenic tobacco plants, the fused enzyme protein was localized primarily in the microsomal fraction. The microsomal monooxygenase activities were approximately 10 times higher toward both 7-ethoxycoumarin and benzo[a]pyrene than in the control plant. The transgenic plants also showed resistance to the herbicide chlortoluron.
Shiota
N
N
Graduate School of Science and Technology, Kobe University, Japan.
Nagasawa
A
A
Sakaki
T
T
Yabusaki
Y
Y
Ohkawa
H
H
D000818:Animals; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D005822:Genetic Vectors; D006540:Herbicides / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D009251:NADPH-Ferrihemoprotein Reductase / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
D017931:DNA Primers; D004274:DNA, Recombinant; D006540:Herbicides; D011993:Recombinant Fusion Proteins; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase