A physical map across chromosome 11q22-q23 containing the major locus for ataxia telangiectasia.
We have constructed a long-range physical map for 12 markers, including genes for GRIA4, IL1BC, and ACAT, across 9 Mb of chromosome 11q22-q23 in the region of the major locus for ataxia-telangiectasia (A-T). The markers fall into proximal and distal groups with respect to the centromere. We have linked the proximal and distal groups by hybridization to a 2.7-Mb NotI fragment and a 4.6-Mb MluI fragment. The following locus order was obtained: centromere-CJ52.75-J12.1C2-Y11B11R-IL1BC-+ ++hbcDNA-GRIA4-CJ52.3-Y11B29L-ACAT- CJ52.193-J12.8-Y11B06R-telomere. We show that hbcDNA/GRIA4 and CJ52.3 are very closely linked to each end, respectively, of the 2.7-Mb NotI fragment, thereby fixing the position of the complete contig. Our results indicate that the gene for A-T is flanked by the markers GRIA4 and J12.8, which are no more than 3 Mb apart, on a 4.6-Mb MluI fragment. The physical map allows rapid positioning of markers, and this will facilitate the construction of a YAC contig across the region.
Ambrose
H J
HJ
CRC Department of Cancer Studies, Medical School, University of Birmingham, United Kingdom.
Byrd
P J
PJ
McConville
C M
CM
Cooper
P R
PR
Stankovic
T
T
Riley
J H
JH
Shiloh
Y
Y
McNamara
J O
JO
Fukao
T
T
Taylor
A M
AM
D001260:Ataxia Telangiectasia / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002503:Centromere; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D008126:Lod Score; D008214:Lymphocytes; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D015183:Restriction Mapping
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA