Aerobic biodegradation of 3-aminobenzoate by gram-negative bacteria involves intermediate formation of 5-aminosalicylate as ring-cleavage substrate.
The aerobic metabolism of 3-aminobenzoate by bacteria was studied. Bacterial strains degrading 3-aminobenzoate were obtained by enrichment with 3-aminobenzoate (strain Ia3) or 5-aminosalicylate (strains BN9 and 5AS1). During growth with 3-aminobenzoate, strain Ia3 and strain 5AS1 transiently accumulated 5-aminosalicylate in the culture broth. In the presence of inhibitors of 5-aminosalicylate 1,2-dioxygenase, resting cells of all three strains converted 3-aminobenzoate to stoichiometric amounts of 5-aminosalicylate. 5-Aminosalicylate 1,2-dioxygenase activity was induced in all strains after growth with 3-aminobenzoate or 5-aminosalicylate, but not after growth in complex media.
Institut für Mikrobiologie, Universität Stuttgart, FRG.
R
R
Russ
C
C
Müller
H J
HJ
Knackmuss
A
A
Stolz
D062365:Aminobenzoates / Q000378:metabolism*; D000636:Aminosalicylic Acids / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D019804:Mesalamine; D010084:Oxidation-Reduction; D062368:meta-Aminobenzoates
D062365:Aminobenzoates; D000636:Aminosalicylic Acids; D003470:Culture Media; D062368:meta-Aminobenzoates; D019804:Mesalamine; C044855:3-aminobenzoic acid