Separation of selenium analogues of sulphur-containing amino acids by high-performance liquid chromatography and high-resolution gas chromatography.
The historically conditioned adaptation of living organisms to chemically corresponding elements is influenced in nature by anthropogenic activities in many regions, the selenium-sulphur pair being one example of such a case. The separation of selenomethionine, selenoethionine and selenocystine was studied by HPLC and high-resolution GC. Ion-exchange chromatography followed by temperature-programmed GC gives the possibility of the analytical separation of trace amounts of selenomethionine in a complex mixture of common amino acids. Diastereoisomers of selenocystine were identified by HPLC in the AccQ-Tag mode.
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno.
J
J
Janák
H A
HA
Billiet
J
J
Frank
K C
KC
Luyben
P
P
Husek
D000603:Amino Acids, Sulfur / Q000737:chemistry* / Q000302:isolation & purification; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D012643:Selenium / Q000737:chemistry*; D013237:Stereoisomerism
D000603:Amino Acids, Sulfur; D012643:Selenium