Heterogeneity of Wistar rats with respect to acid beta-D-glucuronidase (EC 3.2.1.31) in liver.
Commercially available Wistar rats are genetically heterogeneous with respect to acid beta-D-glucuronidase (EC 3.2.1.31) in liver tissue: Of 43 rats studied, 27 animals exhibited only approximately 40% catalytic activity of the remaining group. Analysis by subcellular and density gradient fractionation, and polyacrylamide gel electrophoresis techniques showed that livers with high activity exhibit a dual enzyme distribution in lysosomes (app. 76%) and endoplasmic reticulum (named "microsomal enzyme form"; app. 24%), whereas those with low activity not only lack the microsomal enzyme form - presumably due to the absence of egasyn - but also display reduced lysosomal enzyme activity.
Max-Planck-Institut für Experimentelle Medizin, Göttingen, Germany.
B M
BM
Löffler
E
E
Mielenz
H
H
Kunze
D000818:Animals; D002384:Catalysis; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005966:Glucuronidase / Q000378:metabolism*; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009439:Neuraminidase / Q000494:pharmacology; D017830:Octoxynol / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar / Q000235:genetics*
D007527:Isoenzymes; D017830:Octoxynol; D009439:Neuraminidase; D005966:Glucuronidase