Segregation study of the soluble 39-kD HLA class I heavy chain.
To study the variable expression and inheritance of the 39-kD M(r) form of HLA class I molecules, we investigated the plasma of 12 families with 61 members and 41 unrelated individuals by SDS gel electrophoresis and class-I-specific immunoblotting. Seven families were informative for the presence and absence of the 39-kD band with a clear segregation pattern in coupling with HLA. The data were in agreement with a codominant expression of this molecular variant of sHLA class I. Of the 48 independent HLA haplotypes, 19 were identified as carrying the 39-kD deficiency. However, there was no clear association with HLA-B7 or gender. Under the assumption that all haplotypes in the five non-informative families carried the expressed 39-kD variant, approximately 40% of the Caucasian population should be heterozygous deficient for the 39-kD band of sHLA class I.
Institute of Immunology, Essen University Hospital, Germany.
B S
BS
Kubens
M
M
Pässler
H
H
Grosse-Wilde
D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015870:Gene Expression; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008297:Male; D008970:Molecular Weight; D012995:Solubility
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I