Characterization of a repetitive element detected by NheI in the genomes of Salmo species.
The genomes of the two species in the genus Salmo (Atlantic salmon, S. salar; brown trout, S. trutta) contain a 380-bp repetitive element that is flanked by the recognition sequence of the restriction enzyme NheI. These elements, which comprise approximately 1.2% of the salmon genome, do not exist in long tandem arrays as is typical of satellite DNA. A comparison of the sequences of 16 salmon and 7 trout elements revealed that members of this family of repetitive DNA are closely related to one another (over 95% identity). Subfamily structure exists and there is evidence that members of the same subfamilies are found in both Salmo species. A search of the GenBank database indicated that sequences homologous to the NheI repeat are located within a 1424-bp segment inserted immediately downstream of the 5' end of a Tc1 transposon-like sequence isolated from Atlantic salmon (A.R. Radice, B. Bugaj, D.H. Fitch, and S.W. Emmons, unpublished data; GenBank accession No. L12206).
Department of Biochemistry, Memorial University, St. John's, NF, Canada.
J L
JL
Goodier
W S
WS
Davidson
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D015252:Deoxyribonucleases, Type II Site-Specific; D016678:Genome; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D012474:Salmon / Q000235:genetics*; D012487:Salmonidae / Q000145:classification / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014337:Trout / Q000235:genetics*
D004251:DNA Transposable Elements; D004247:DNA; C067043:endodeoxyribonuclease NheI; D015252:Deoxyribonucleases, Type II Site-Specific