Porphyromonas gingivalis fimbriae induce a 68-kilodalton phosphorylated protein in macrophages.
The present study was performed to examine whether Porphyromonas gingivalis fimbriae induce specifically a protein kinase-mediated phosphorylated protein that is involved in the mechanism of signal transduction. The fimbriae induced a 68-kDa phosphorylated protein (pp68) in a dose-dependent manner in mouse peritoneal macrophages. A marked appearance of pp68 was observed 20 min after the initiation of fimbrial treatment. The fimbria-induced pp68 was inhibited dramatically by staurosporine, a potent inhibitor of protein kinase C. pp68 induction was also inhibited by H-7, a potent inhibitor of several types of protein kinase. However, the induction was not inhibited by HA-1004 and H-8, relatively high-affinity inhibitors of protein kinase A. Phorbol myristate acetate and 1-oleoyl-2-acetyl-sn-glycerol, activators of protein kinase C, were able to induce pp68 in mouse peritoneal macrophages. This protein was localized in the cytosolic fraction of fimbria-treated macrophages. pp68 also was induced in fimbria-treated human monocyte-like cells. Finally, we observed that gene expression of the fimbria-induced neutrophil chemoattractant KC was inhibited markedly by staurosporine.
Department of Oral Microbiology, Meikai University School of Dentistry, Saitama, Japan.
Y
Y
Murakami
S
S
Hanazawa
A
A
Watanabe
K
K
Naganuma
H
H
Iwasaka
K
K
Kawakami
S
S
Kitano
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002451:Cell Compartmentation; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D002634:Chemotaxis, Leukocyte; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D016207:Cytokines / Q000096:biosynthesis; D003600:Cytosol / Q000737:chemistry; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D009000:Monocytes / Q000276:immunology; D010750:Phosphoproteins / Q000096:biosynthesis*; D016966:Porphyromonas gingivalis / Q000276:immunology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction; D019311:Staurosporine; D013449:Sulfonamides
D000470:Alkaloids; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; C475895:Cxcl1 protein, mouse; D016207:Cytokines; D007546:Isoquinolines; D010750:Phosphoproteins; D013449:Sulfonamides; C074302:keratinocyte-derived chemokines; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D019311:Staurosporine