Flow injection spectrophotometric determination of aspartame in dietary products.
A flow injection spectrophotometric method has been developed for the determination of aspartame in dietary products using ninhydrin as a colorimetric reagent. The reaction was conducted in a 1 + 1 v/v methanol-isopropanol medium also containing potassium hydroxide. The absorbance measurements were made at 603 nm. The results obtained for the determination of aspartame in table sweetener, pudding, gelatin, and refreshment (i.e., a powder dissolved in water for drinking) are in good agreement with the results obtained using a conventional manual procedure (correlation coefficient, r = 0.9984). Thirty-six results were obtained per hour, and the relative standard deviation was less than 3.5% (n = 6) for all samples. The detection limit (three times the signal blank/slope) was 3.8 x 10(-5) mol l-1 of aspartame.
Departamento de Química, Universidade Federal de São Carlos, SP, Brazil.
J de A
Jde A
Nóbrega
O
O
Fatibello-Filho
I da C
Ida C
Vieira
D001218:Aspartame / Q000032:analysis*; D017022:Flow Injection Analysis; D005504:Food Analysis; D013053:Spectrophotometry
D001218:Aspartame