Identification of common antigens of serotype 1 and serotype 3 Pasteurella multocida in poultry expressed in vivo.
Eight groups of chicken immune sera and one group of turkey immune sera were used to identify unique in vivo-expressed antigens of Pasteurella multocida by Western immunoblotting. When antisera were prepared against the detergent-insoluble fraction of in vivo-grown P-1059, live X-73, live P-1059, live 86-1913, live P-1662, and live P-1702, they recognized four major antigens on immunoblots of the detergent-insoluble fraction of in vivo-grown serotype 1 and in vivo-grown serotype 3. These four antigens, which have approximate molecular weights of 204,000, 192,000, 179,000, and 153,000, were not recognized by antisera made against the detergent-soluble fraction of in vivo-grown P-1059, whole in vitro-grown inactivated X-73, or whole in vitro-grown inactivated P-1059. The results provide evidence for common antigens expressed by in vivo-grown serotype 1 and in vivo-grown serotype 3. These antigens are recognized by sera from chickens that have survived infection by various serotypes. The antigens are concentrated in the detergent-insoluble fraction of in vivo-grown bacteria and likely represent unique in vivo-expressed outer-membrane proteins.
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602-4875.
C
C
Wang
J R
JR
Glisson
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D002645:Chickens / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D008970:Molecular Weight; D016979:Pasteurella multocida / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D011200:Poultry / Q000276:immunology*; D012703:Serotyping; D014422:Turkeys / Q000276:immunology
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007106:Immune Sera