[Study of the dynamics repair of DNA damage caused by N-methyl-N-nitrosourea during activated NAD biosynthesis in rat liver].
Dynamics of the DNA main reparative reactions was studied in rat liver tissue after N-methyl-N-nitrose urea treatment and simultaneous stimulation of NAD biosynthesis in order to evaluate the interrelationship between DNA repair and poly-ADP-ribosylation. Treatment of liver tissue with the mutagen was followed by the preparations incubation in Hanks solution within 30 min at 37 degrees. During the initial steps of restoration an increase in amount of DNA breaks proved to occur in liver tissue of the experimental animals as compared with controls; these deteriorations were decreased down to initial level to the final steps of incubation, while content of breaks in single-strand DNA remained to be high in liver tissue of control animals. The intensive rate of DNA repair in rat liver tissue containing high level of NAD correlated with an increase in nuclear endonuclease and DNA-dependent-DNA-beta-polymerase activities. At the same time, the activity of poly-ADP-ribosyl polymerase was only slightly altered during all the steps of the tissue repair studied.
S M
SM
Vasil'eva
N A
NA
Muliavko
G V
GV
Donchenko
D000818:Animals; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004256:DNA Polymerase I / Q000378:metabolism; D004260:DNA Repair; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008770:Methylnitrosourea / Q000633:toxicity*; D009243:NAD / Q000096:biosynthesis* / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D051381:Rats
D009687:Nuclear Proteins; D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose; D008770:Methylnitrosourea; D004247:DNA; D004256:DNA Polymerase I
Izuchenie dinamiki reparatsii povrezhdeniĭ DNK, vyzvannykh N-metil- N-nitrozomochevinoĭ v usloviiakh aktivirovannogo biosinteza NAD v pecheni krys.