[Interstitial collagen distribution and its mRNA expression in human primary liver cancer].
The localization of type I collagen and type III procollagen in 29 cases of hepatocellular carcinoma (HCC) and 2 cases of cholangiocarcinoma as well as their surrounding tissues were studied with ABC method. The results showed that the distribution of these two types of collagen was similar in PLC as well as in its surrounding tissues. The distribution of interstitial collagen in HCC might be classified into two patterns by the authors' namely, the vasiform and non-vascular distributions. The carcinoma with vasiform distribution pattern tends to grow infiltratively with much higher rate of portal venous embolism and intrahepatic metastasis development than that of the non-vascular pattern (P < 0.05). Employing the in situ hybridization technique for type I collagen mRNA, the transcription of collagen mRNA occurred not only in the interstitial cells but also in some of the liver carcinoma cells and giant tumor cells. No positive signals of type I collagen mRNA were noticed in the non-tumorous surrounding tissues.
Dai
Y M
YM
2nd Military Medical University, Shanghai.
Wang
Y J
YJ
Ni
C R
CR
D001650:Bile Duct Neoplasms / Q000737:chemistry / Q000235:genetics; D001653:Bile Ducts, Intrahepatic; D006528:Carcinoma, Hepatocellular / Q000737:chemistry* / Q000235:genetics; D018281:Cholangiocarcinoma / Q000737:chemistry / Q000235:genetics; D003094:Collagen / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008113:Liver Neoplasms / Q000737:chemistry* / Q000235:genetics; D011347:Procollagen / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*
D011347:Procollagen; D012333:RNA, Messenger; D003094:Collagen