Narrow-bore liquid chromatographic assay for oxcarbazepine and its major metabolite in rat brain, liver and blood microdialysates.
An isocratic narrow-bore high-performance liquid chromatographic assay with UV detection is described for the detection of oxcarbazepine and its major metabolite, 10,11-dihydro-10-hydroxycarbamazepine, using carbamazepine as the internal standard. This method is sufficiently sensitive to allow quantification of oxcarbazepine and its metabolite in rat brain, liver and blood microdialysates.
Department of Pharmaceutical Chemistry and Drug Analysis, Vrije Universiteit Brussels, Belgium.
K
K
Van Belle
V
V
De koster
S
S
Sarre
G
G
Ebinger
Y
Y
Michotte
D000818:Animals; D000927:Anticonvulsants / Q000032:analysis* / Q000097:blood; D001923:Brain Chemistry; D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods* / Q000706:statistics & numerical data; D003956:Dialysis; D008099:Liver / Q000737:chemistry*; D008297:Male; D000078330:Oxcarbazepine; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
C039775:10,11-dihydro-10-hydroxycarbamazepine; D000927:Anticonvulsants; D002220:Carbamazepine; D000078330:Oxcarbazepine