[Evaluation of anti-rheumatoid arthritis 36 kD and anti-33 kD antibodies in the diagnosis of rheumatoid arthritis].
By using soluble nuclear extracts from Ehrlich mice ascite cells, immunoblot analysis showed reactivity of rheumatoid arthritis (RA) serum with a 36 kD band. It was found that 28 of 88 patients with RA had anti-RA 36 kD antibody (31.8%), but there was no reactivity in the serum samples from 100 patients with other connective tissue diseases and 40 normal controls. However, the nuclear antigen mentioned above could react with either anti-RA 33 kD antibody or anti-RNP antibody in the 33 kD band. Thus, anti-RA 33 kD antibody was not a specific antibody for RA. Anti-RA 36 kD antibody appeared to be a marker antibody in RA. If anti-RA 36 kD and anti-33 kD antibodies appeared in a serum sample simultaneously, it might be more valuable for the diagnosis of RA.
Zhou
Z J
ZJ
Department of Medicine, Peking Union Medical College Hospital, Beijing.
Jiang
M
M
Song
Q F
QF
D000974:Antibodies, Antinuclear / Q000097:blood*; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D006801:Humans; D015151:Immunoblotting
D000974:Antibodies, Antinuclear; D015415:Biomarkers