Iodixanol: a nonionic iso-osmotic centrifugation medium for the formation of self-generated gradients.
The physical and biological properties of Iodixanol, a new nonionic density gradient medium, are described in this paper. It is effectively a dimer of Nycodenz and it exhibits two significant advantages over previous iodinated density gradient media--its aqueous solutions are iso-osmotic up to a density of 1.32 g/ml and it is capable of forming self-generating gradients in 1 to 3 h. It has a very low toxicity toward biological material and enzyme assays can be carried out in its presence.
Ford
T
T
Department of Biology, University of Essex, Colchester, United Kingdom.
Graham
J
J
Rickwood
D
D
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002499:Centrifugation, Density Gradient / Q000379:methods*; D003287:Contrast Media; D006801:Humans; D007202:Indicators and Reagents; D007472:Iohexol; D014283:Triiodobenzoic Acids / Q000737:chemistry / Q000633:toxicity
D003287:Contrast Media; D007202:Indicators and Reagents; D014283:Triiodobenzoic Acids; D007472:Iohexol; C044834:iodixanol