Comparison of microscopic and flow cytometric detection of platelet antibody in dogs suspected of having immune-mediated thrombocytopenia.
A flow cytometric platelet immunofluorescence assay (FC-PIFA) was compared with a previously developed microscopic platelet immunofluorescence assay (MI-PIFA) for detection of circulating platelet antibody. Both assays were performed on serum from 10 healthy dogs with normal platelet count, and on serum from 27 thrombocytopenic dogs--18 had primary immune-mediated thrombocytopenia (IMT), and 9 had IMT in addition to other immune-mediated disease (secondary IMT). Both assays yielded negative results for all control dogs. The MI-PIFA and FC-PIFA results were in agreement in 23 dogs with IMT (14 positive and 9 negative). There was linear correlation between MI-PIFA scores and FC-PIFA results (r = 0.873). Positive results were obtained for 55.5% of the dogs with suspected IMT, using the MI-PIFA, compared with 67%, using the FC-PIFA; however, the difference was not statistically significant. Use of fresh or frozen fixed donor platelets as the antigen source yielded similar results in the FC-PIFA.
Kristensen
A T
AT
Department of Small Animal Clinical Sciences, University of Minnesota, St Paul 55108.
Weiss
D J
DJ
Klausner
J S
JS
Laber
J
J
Christie
D J
DJ
D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D001792:Blood Platelets / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis / Q000209:etiology / Q000276:immunology*; D004285:Dogs; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique / Q000706:statistics & numerical data / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood; D012680:Sensitivity and Specificity; D013921:Thrombocytopenia / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D001323:Autoantibodies; D007074:Immunoglobulin G