Liquid chromatographic-mass spectrometric analysis of N-acetylamino acids in human urine.
Liquid chromatography-atmospheric pressure chemical-ionization mass spectrometry (LC-APCI-MS) was used for the analysis of N-acetylamino acids that could not be determined with an amino acid analyzer. LC-APCI-MS could directly detect the protonated molecular ions of various synthetic N-acetylamino acids, distinguishing N-acetylserine from O-acetylserine and N(alpha)-acetyllysine from N(epsilon)- acetyllysine. Furthermore, N-acetylasparagine, N-acetylaspartic acid, N-acetylglutamine and N-acetylglutamic acid were identified in normal human urine. The assay data for N-acetylaspartic acid agree with a previous report using gas chromatography-mass spectrometry. These results demonstrate the usefulness of the apparatus described above for the analysis of N-acetylamino acids in biological samples.
Sugahara
K
K
Department of Chemistry, Kochi Medical School, Japan.
Zhang
J
J
Kodama
H
H
D000107:Acetylation; D000596:Amino Acids / Q000652:urine*; D001216:Asparagine / Q000031:analogs & derivatives / Q000652:urine; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D005971:Glutamates / Q000652:urine; D005973:Glutamine / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000652:urine; D013058:Mass Spectrometry / Q000379:methods*; D012044:Regression Analysis; D012694:Serine / Q000031:analogs & derivatives / Q000652:urine
D000596:Amino Acids; D005971:Glutamates; C032007:aceglutamide; D005973:Glutamine; C002315:N(alpha)-acetyllysine; D001224:Aspartic Acid; D012694:Serine; D001216:Asparagine; C000179:N-acetylaspartate; C026988:N-acetylasparagine; D008239:Lysine; C016195:N-acetylglutamic acid; C012162:N-acetylserine