Micellar electrokinetic capillary chromatography of 8-hydroxydeoxyguanosine and other oxidized derivatives of DNA.
8-Hydroxydeoxyguanosine (8-OH-dG) is widely recognized as a marker of DNA oxidation. Until now, 8-OH-dG has been measured by high-performance liquid chromatography or by gas chromatography-mass spectrometry. A method is reported that detects oxidative derivatives of deoxynucleosides by micellar electrokinetic capillary chromatography. Single-stranded DNA was incubated in the presence of 50 mM hydrogen peroxide-10 mM ascorbic acid and hydrolysed by enzymatic digestion. The order of electrophoretic mobilities of deoxynucleosides was dC > dA > T > dG > 8-OH-dG. 8-OH-dG was determined by introducing a laboratory-prepared internal standard. Two additional major oxidative derivatives were identified by comparing the electropherogram of the oxidized DNA with that of the oxidized standard deoxyguanosine.
Guarnieri
C
C
Department of Biochemistry G. Moruzzi, University of Bologna, Italy.
Muscari
C
C
Stefanelli
C
C
Giaccari
A
A
Zini
M
M
Di Biase
S
S
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002845:Chromatography; D018736:DNA Adducts / Q000737:chemistry / Q000302:isolation & purification*; D004277:DNA, Single-Stranded / Q000737:chemistry; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D004563:Electrochemistry; D004586:Electrophoresis; D008823:Micelles; D009691:Nucleic Acid Denaturation; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet
D018736:DNA Adducts; D004277:DNA, Single-Stranded; D008823:Micelles; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine