Induction of chemotactic and invasive phenotype in BALB/c 3T3 cells by 1,2-dibromoethane transformation.
1,2-Dibromoethane (DBE), which can act as initiating agent, is capable of inducing a malignant phenotype in BALB/c 3T3 cells. Cells transformed with a single noncytotoxic dose formed more progressive tumors in vivo. Almost all animals (95%) receiving the inoculum of two different transformed clones developed tumors within 1 month. In the control group only 55% of the animals developed tumors after 4 months. Treatment with DBE also increased the chemotactic properties of target cells, which also acquired ability to penetrate and colonize a reconstituted basal membrane (matrigel). These data suggest that DBE could play a key role in tumor progression.
Istituto Nazionale per la Ricerca sul Cancro (IST-GE), Genova, Italia.
R
R
Adatia
A
A
Albini
A
A
Colacci
C
C
De Giovanni
P
P
Nanni
D
D
Giunciuglio
S
S
Parodi
S
S
Grilli
P
P
Perocco
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002633:Chemotaxis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015946:Ethylene Dibromide / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology*; D010641:Phenotype; D013997:Time Factors
D015946:Ethylene Dibromide