Detecting the ability of viral, bacterial and eukaryotic replication proteins to track along DNA.
The phage T4 gene 45 protein (gp45), Escherichia coli beta and the eukaryotic proliferating cell nuclear antigen (PCNA) function in replication as processivity factors of their corresponding DNA polymerases. The T4 gp45 also functions as the transcriptional activator that connects expression of viral late genes to DNA replication. DNA tracking is an essential component of the replication and transcription regulatory functions of T4 gp45. The ability of gp45, beta and PCNA to track along DNA has been analyzed by photocrosslinking. Each of these proteins must be loaded onto DNA by a species-specific assembly factor. For gp45 and beta, the density of traffic along DNA is determined by a dynamic balance between continuous protein loading and unloading, and is also dependent on interaction with the conjugate single-stranded DNA binding protein.
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093-0634.
R L
RL
Tinker
G A
GA
Kassavetis
E P
EP
Geiduschek
D001386:Azides / Q000378:metabolism / Q000528:radiation effects; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004258:DNA Polymerase III / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010777:Photochemistry; D011387:Prokaryotic Cells / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D013045:Species Specificity; D015534:Trans-Activators / Q000378:metabolism; D014542:Uridine Monophosphate / Q000031:analogs & derivatives / Q000378:metabolism / Q000528:radiation effects; D014764:Viral Proteins / Q000378:metabolism
D001386:Azides; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D046911:Macromolecular Substances; D018809:Proliferating Cell Nuclear Antigen; D015534:Trans-Activators; D014764:Viral Proteins; C074855:gene 44 protein, Enterobacteria phage T4; C074555:gene 45 protein, Enterobacteria phage T4; C064622:5-(N-(4-azidobenzoyl)-3-aminoallyl)deoxyuridine 5'-monophosphate; D004247:DNA; D014542:Uridine Monophosphate; D004258:DNA Polymerase III