Fc epsilon RI-stimulated Ca(2+)-dependent secretion from rat basophilic leukemia (RBL-2H3) cells permeabilized with Staphylococcal alpha-toxin: Fc epsilon RI-operated signals are not mimicked by the actions of GTP gamma S.
1. RBL-2H3 cells permeabilized with alpha-toxin responded to dinitrophenol (30-40 mol/mol)-conjugated human serum albumin, as antigen, to secrete [14C]serotonin in the micromolar range of free Ca2+. 2. Calcium ion alone did not cause substantial secretion. 3. Guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) (100 microM) in combination with Ca2+ produced only negligible [14C]serotonin secretion. 4. GTP gamma S, in the presence of cytochalasin D, caused optimal secretion of [14C]serotonin in a Ca(2+)-dependent manner.
Department of Molecular Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.
K
K
Oishi
T
T
Uekusa
T
T
Iwasaki
Y
Y
Fukuda
H
H
Ikeya
R
R
Inoue
M K
MK
Uchida
D000225:Adenine / Q000378:metabolism; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism*; D051381:Rats; D017455:Receptors, IgE / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism; D014407:Tumor Cells, Cultured; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D017455:Receptors, IgE; C021091:staphylococcal alpha-toxin; D012701:Serotonin; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D001619:beta-N-Acetylhexosaminidases; D000225:Adenine; D002118:Calcium