pH-independent inhibition of restriction endonuclease cleavage via triple helix formation by oligonucleotides containing 8-oxo-2'-deoxyadenosine.
The ability of homopyrimidine oligonucleotides containing 8-oxo-2'-deoxyadenosine to form stable, triple helical structures with the sequence containing the recognition site for the class II-S restriction enzyme, Ksp632-I, was studied as a function of pH. The 8-oxo-2'-deoxyadenosine-substituted oligomers were shown to inhibit enzymatic cleavage and to bind within the physiological pH range in a pH-independent fashion without compromising specificity.
Wang
Q
Q
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Tsukahara
S
S
Yamakawa
H
H
Takai
K
K
Takaku
H
H
D001483:Base Sequence; D003839:Deoxyadenosines / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000037:antagonists & inhibitors*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*
D003839:Deoxyadenosines; D009838:Oligodeoxyribonucleotides; C078251:2'-deoxy-7,8-dihydro-8-oxoadenosine; C057073:endodeoxyribonuclease Ksp632I; D015252:Deoxyribonucleases, Type II Site-Specific