Inositol 1,4,5-trisphosphate receptor function in neonatal cardiomyocytes.
We have previously reported that the metabolism of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3) is altered when rat neonatal ventricular cardiomyocytes are isolated and cultured. In the current study we show that the mass content of Ins(1,4,5)P3 is lower in the isolated cells than in the intact tissue. However, the properties of the Ins(1,4,5)P3 receptors were not different in the two preparations and the isolated cells remained insensitive to Ins(1,4,5)P3 in terms of 45Ca2+ release. Thus, despite the altered pattern of metabolism of Ins(1,4,5)P3 in isolated neonatal cells, the properties of the receptors were similar to those reported in other myocardial preparations.
Cellular Biochemistry Laboratory, Baker Medical Research Institute, Prahran, Victoria, Australia.
M
M
Fitzgerald
K E
KE
Anderson
E A
EA
Woodcock
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000032:analysis / Q000502:physiology*; D015544:Inositol 1,4,5-Trisphosphate / Q000032:analysis / Q000378:metabolism*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011921:Rats, Inbred WKY; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis / Q000502:physiology*
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium