Gram-positive cell wall structure of the A3 gamma type in heliobacteria.
The amino acid composition and structure of the peptidoglycan from Heliobacillus mobilis was determined by one- and two-dimensional thin-layer chromatography of completely and partially hydrolysed cell wall preparations. The structure was found to be of the A3 gamma type, with L,L-diaminopimelate in position 3, D-alanine in position 4 and a glycine interpeptide bridge, as found in certain groups of Gram-positive bacteria including Clostridium perfringens and Nocardioides simplex. The presence of a Gram-positive type of cell wall in heliobacteria is consistent with their phylogenetic relationship to the 'low G + C' Gram-positive bacteria, as previously demonstrated by 16S rRNA sequencing.
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, UK.
M W
MW
Pickett
N
N
Weiss
D J
DJ
Kelly
D000595:Amino Acid Sequence; D002473:Cell Wall / Q000737:chemistry*; D006094:Gram-Positive Bacteria / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry*; D010802:Phylogeny
D010457:Peptidoglycan