Non-extractive metabolism study of E and A destruxins in the locust, Locusta migratoria L. III. Direct high-performance liquid chromatographic analysis and parallel fast atom bombardment mass spectrometric monitoring.
To study the behavior of the toxins E and A destruxin in biological media, a method was developed with direct injection on to a liquid chromatographic (LC) column. Either slightly lipophilic C1 and C4 "wide-pore" packings or the column-switching approach with a guard column were used. To confirm the results of the direct LC analysis, a "classical approach" with pretreatment prior to injection on to a C8 packed column was also developed. Further, parallel fast atom bombardment MS monitoring in the negative-ion mode was carried out on the same biological samples, to obtain complementary information on the destruxin metabolism in locusts. Thus, the behaviours of E and A destruxin were examined in vivo in different organs of locusts. For E destruxin, several detoxication processes could be observed, such as hydrolysis and conjugation with glutathione.
Loutelier
C
C
Laboratoire de Chimie Organique Structurale, Université P. et M. Curie, Paris, France.
Lange
C
C
Cassier
P
P
Vey
A
A
Cherton
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides; D005216:Fat Body / Q000737:chemistry; D005656:Fungal Proteins; D006110:Grasshoppers / Q000378:metabolism*; D006458:Hemolymph / Q000737:chemistry; D007202:Indicators and Reagents; D008297:Male; D008317:Malpighian Tubules / Q000737:chemistry; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000032:analysis / Q000493:pharmacokinetics*; D010456:Peptides, Cyclic / Q000032:analysis / Q000493:pharmacokinetics*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet
D047630:Depsipeptides; D005656:Fungal Proteins; D007202:Indicators and Reagents; D009183:Mycotoxins; D010456:Peptides, Cyclic; C040423:destruxin A; C040425:destruxin E