[Detection of the direct genotyping of HSV by PCR and its primal usage in ophthalmology].
The direct genotyping of Herpes Simplex Virus (HSV) by polymerase chain reaction (PCR) was used to analysis the smears from corneas or tears of 37 cases suspected HSK. The results show that PCR is a sensitive rapid and simple method. The key procedure is taking specimens.
Lab of Molecular Biology, 2nd Medical University of Shanghai, China.
W
W
Xu
S
S
Chen
Z
Z
Wang
Z
Z
Zhou
C
C
Li
Y
Y
Zheng
B
B
Chen
D003315:Cornea / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005838:Genotype; D006801:Humans; D016849:Keratitis, Herpetic / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D018139:Simplexvirus / Q000235:genetics* / Q000302:isolation & purification; D013666:Tears / Q000382:microbiology
D004279:DNA, Viral