Human Th0-type T helper-cell clone supports antigen-specific immunoglobulin production in scid/beige-hu mice.
Tetanus toxoid-specific T cells have been generated from human splenic lymphocytes by an initial 6-day stimulation period with antigen, followed by a proliferation period with recombinant IL-2 and human feeder cells. Proliferating T cells were subsequently cloned by limiting dilution. A human T-cell clone that was functionally characterized showed: (i) a specific proliferative response to tetanus toxoid in the presence of autologous Epstein-Barr virus (EBV)-transformed lymphoblastoid cells; (ii) a phenotype characteristic for the helper/inducer CD4+/CD8-/CD450R0+ T cells, and (iii) a lymphokine profile, as determined by mRNA analysis, representative of Th0-like human CD4+ T helper cells. This tetanus toxoid-specific T-cell clone which showed antigen-dependent helper activity for antibody production by autologous B cells in vitro could also provide T-cell help to antigen-specific human B cells transplanted into severe combined immunodeficiency (scid/beige) mice.
Chin
L T
LT
Department of Immunotechnology, Lund University, Sweden.
Ifversen
P
P
Kristensson
K
K
Wahren
B
B
Carlsson
R
R
Borrebaeck
C A
CA
D000368:Aged; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000637:transplantation; D001483:Base Sequence; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D017493:Leukocyte Common Antigens / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D017710:Lymphocyte Transfusion / Q000379:methods*; D008222:Lymphokines / Q000096:biosynthesis; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology
D008222:Lymphokines; D012333:RNA, Messenger; D013745:Tetanus Toxoid; D017493:Leukocyte Common Antigens