Analysis of the transfer region of the Streptomyces plasmid SCP2.
pIJ903, a bifunctional derivative of the 31.4 kb low-copy-number, conjugative Streptomyces plasmid SCP2*, was mutagenized in Streptomyces lividans using Tn4560. Mutant plasmids differing in their transfer frequencies, chromosome mobilization abilities, pock formation, and complementation properties were isolated. The mutations defined five transfer-related genes, traA, traB, traC, traD and spd, clustered in a region of 9 kb. The deduced sequences of the putative TraA and TraB proteins showed no overall similarity to known protein sequences, but the phenotype of traA mutant plasmids and sequence motifs in the putative TraA protein suggested that it might be a DNA helicase.
John Innes Institute, John Innes Centre, Norwich, UK.
D F
DF
Brolle
H
H
Pape
D A
DA
Hopwood
T
T
Kieser
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003227:Conjugation, Genetic / Q000235:genetics*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D013291:Streptococcus / Q000235:genetics*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C090132:TraA protein, Streptomyces; C082576:traB protein, Bacteria; C074790:traC protein, Plasmid F; C064957:traD protein, E coli; D036781:Fimbriae Proteins