Analysis of the double-stranded RNA genome segments among avian reovirus field isolates.
Nine isolates of avian reovirus (ARV) from both healthy birds and birds with different clinical illness and one commercially available vaccine strain were selected and characterized by analysis of the migration pattern of their genomic double-stranded RNA (dsRNA) segments following separation by polyacrylamide gel electrophoresis. Different electropherotypes were observed and analyzed. The results show that the dsRNA segments of ARV were markedly polymorphic among isolates within the same serotype as well as among different serotypes. The results also show no correlation between electropherotype and disease state.
Wu
W Y
WY
Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.
Shien
J H
JH
Lee
L H
LH
Shieh
H K
HK
D000818:Animals; D002645:Chickens / Q000821:virology; D016679:Genome, Viral; D011201:Poultry Diseases / Q000821:virology*; D011784:Quail / Q000821:virology; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012088:Reoviridae Infections / Q000662:veterinary* / Q000821:virology
D012330:RNA, Double-Stranded; D012367:RNA, Viral