A model for transcription termination by RNA polymerase I.
The transcription termination site for yeast RNA polymerase I requires not only an 11 bp binding site for Reb1p, but also about 46 bp of 5' flanking sequence. We propose that Reb1p bound to its site is part of a pause element, while the 5' flanking sequence contains a release element. Pausing requires little other than the DNA-binding domain of Reb1p and is not specific for polymerase I. The release element, however, can be polymerase specific. We propose a general model for eukaryotic transcription terminators in which termination occurs when a relatively nonspecific signal induces polymerase to pause in the context of a release element.
Lang
W H
WH
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.
Morrow
B E
BE
Ju
Q
Q
Warner
J R
JR
Reeder
R H
RH
D001483:Base Sequence; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005057:Eukaryotic Cells / Q000502:physiology; D005656:Fungal Proteins / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011485:Protein Binding; D012318:RNA Polymerase I / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship; D013728:Terminator Regions, Genetic / Q000235:genetics*; D014158:Transcription, Genetic; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D011994:Recombinant Proteins; D012318:RNA Polymerase I