Two novel HMW MAP2 variants with four microtubule-binding repeats and different projection domains.
The brain microtubule-associated protein MAP2 is composed of two high molecular (MAP2a and b) and one low molecular (MAP2c) weight isoforms. All these forms were thought to contain three repeated microtubule-binding domains in their C-terminal region but a MAP2c variant containing four repeats has recently been identified. We report here the existence of two high molecular weight MAP2 isoforms with four microtubule-binding domains in the sensory neuronal cell line ND 7/23. A stretch of 135 bp is missing in one of these forms suggesting that several HMW MAP2 variants can be produced by alternative splicing.
Langkopf
A
A
Unité INSERUM 282 affiliée au CNRS, Hôpital Henri Mondor, Créteil, France.
Guilleminot
J
J
Nunez
J
J
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation; D002459:Cell Fusion; D014644:Genetic Variation; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000235:genetics; D008870:Microtubules / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009447:Neuroblastoma; D009474:Neurons / Q000737:chemistry; D016133:Polymerase Chain Reaction; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured
D008869:Microtubule-Associated Proteins