Exposure of simian virus-40-transformed human cells to magnetic fields results in increased levels of T-antigen mRNA and protein.
In its integrated form. SV-40 DNA offers an opportunity to observe the behavior of what is in effect a viral genome within a cellular genome, with transcriptional and translational products that can be clearly distinguished from those of the host cell. Exposure of SV40-transformed human fibroblasts to a 60 Hz continuous-wave sinusoidal electromagnetic (EM) field resulted in increased levels of virally derived mRNA and protein of large T-antigen. These findings provide evidence that a foreign DNA integrated into cells can be affected by EM fields under conditions known to cause increased transcripts from endogenous cellular genes.
Gold
S
S
Institute for Biomolecular Structure and Function, Biological Sciences, Hunter College-CUNY, NY 10021.
Goodman
R
R
Shirley-Henderson
A
A
D000957:Antigens, Viral, Tumor / Q000096:biosynthesis*; D015153:Blotting, Western; D002460:Cell Line; D002461:Cell Line, Transformed / Q000528:radiation effects; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects*; D006801:Humans; D008280:Magnetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic / Q000528:radiation effects
D000957:Antigens, Viral, Tumor; D012333:RNA, Messenger