Improved separation of radioactively labelled cellular phospholipids by high-performance liquid chromatography.
An improved high-performance liquid chromatographic method for the separation and determination of radioactively labelled cellular phospholipids is described. The method is based on separation of phospholipids on a 250 x 4 mm I.D. LiChrospher DIOL 100 (5 microns) column, fitted with a 50 x 4 mm I.D. LiChrospher Si 60 (5 microns) precolumn and a gradient of 5% H3PO4 and acetonitrile. It allows the determination of small amounts of labelled phosphatidylcholine and sphingomyelin due to the sharp elution profile in spite of long retention times.
Trümbach
B
B
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany.
Rogler
G
G
Lackner
K J
KJ
Schmitz
G
G
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling; D010713:Phosphatidylcholines / Q000302:isolation & purification; D010743:Phospholipids / Q000302:isolation & purification*; D012867:Skin / Q000737:chemistry / Q000166:cytology; D013056:Spectrophotometry, Ultraviolet; D013109:Sphingomyelins / Q000302:isolation & purification; D013631:Tangier Disease / Q000378:metabolism
D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins