Neurotrophin-3 (NT-3) levels in the developing rat nervous system and in human samples.
We developed an enzyme immunoassay (EIA) system for neurotrophin-3 (NT-3), based on a biotin-streptavidin detection system capable of measuring concentrations as low as 0.5 pg/ml with high reproducibility. We measured NT-3 levels in the developing rat nervous system as well as in normal subjects and patients with Alzheimer's disease (AD). The developmental change of NT-3 level in rat brain and peripheral tissues coincided with the change in NT-3 mRNA level reported before. We could not find any differences in NT-3 level in serum, cerebrospinal fluid, or brain (hippocampus and parietal cortex) obtained from controls and patients with AD.
Department of Molecular Biology, Gifu Pharmaceutical University, Japan.
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Murase
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Igarashi
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Hayashi
D000328:Adult; D000544:Alzheimer Disease / Q000378:metabolism*; D000818:Animals; D001923:Brain Chemistry; D002478:Cells, Cultured; D002490:Central Nervous System / Q000737:chemistry* / Q000254:growth & development*; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008813:Mice, Inbred ICR; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000032:analysis*; D020933:Neurotrophin 3; D051381:Rats; D014781:Viscera / Q000737:chemistry
D009414:Nerve Growth Factors; D020933:Neurotrophin 3