A cDNA encoding the human transforming growth factor beta receptor suppresses the growth defect of a yeast mutant.
Transforming growth factor beta (TGF-beta) is a multifunctional factor that regulates many aspects of cellular processes. TGF-beta signals through a heteromeric complex of type-I and type-II receptors, which both belong to the transmembrane (TM) receptor serine/threonine kinase family. Reported here is the isolation of a subtype of the human TGF-beta receptor type II from a cDNA library using a Saccharomyces cerevisiae mutant. This yeast mutant has a defect in the expression of the gene encoding inositol-1-phosphate synthase and requires myo-inositol for its growth. The cloned subtype of the TGF-beta receptor type II has a 25-amino-acid insertion relative to the reported receptor type-II sequence. In addition to that encoding the TGF-beta receptor, two more human genes were obtained using the same yeast mutant. They encode the protein phosphatase type 2A regulatory subunit A and a 14-3-3 protein which is known as a regulatory protein for protein kinases. These results clearly indicate that these human genes function in yeast cells. It is also suggested that yeast possesses a signal transduction mechanism resembling the human TGF-beta-mediated signaling pathway.
Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan.
J
J
Nikawa
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D015870:Gene Expression; D005800:Genes, Fungal; D016153:Genes, Suppressor; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D007296:Myo-Inositol-1-Phosphate Synthase / Q000096:biosynthesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D013489:Suppression, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D018125:Receptors, Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D007296:Myo-Inositol-1-Phosphate Synthase