The construction of a beta-lactamase-encoding ApR gene cassette flanked by recognition sites for NotI, SacII, MluI, SplI, BssHII and NarI.
An ApR gene cassette was constructed using the gene present in the PBR322 derivative pTZ18. The cassette is maintained in the plasmid pcLINK-1 and may be excised with any of the six rare cutting enzymes NotI, SacII, MluI, SplI, BssHII or NarI. By using a double-digestion procedure, the ApR gene may be excised with two different protruding ends. In the process of constructing the pcLINK-1 plasmid the multiplecloning site of pTZ18 was extended with recognition sites for the enzymes NotI, SacII, MluI, SplI, BssHII and NarI.
Höglund
M
M
Genome Analysis Laboratory, Imperial Cancer Research Fund, London, UK.
Lehrach
H
H
D001483:Base Sequence; D001665:Binding Sites; D004274:DNA, Recombinant; D004720:Endonucleases / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics*
D004274:DNA, Recombinant; D004720:Endonucleases; D001618:beta-Lactamases