[Cytoprotective effect of neurotensin on acetaminophen induced liver injury in relation to glutathione system].
In the present work the cytoprotective effect of neurotensin (NT) on acetaminophen induced injury to the liver or cultured hepatocytes of mouse in relation to glutathione system were investigated. The results were as follows: Pretreatment with NT significantly reduced the leakages of transaminases induced by acetaminophen in vivo or in cultured hepatocytes and partially reversed the decline of DNA synthesis induced by acetaminophen in cultured hepatocytes. After administration of acetaminophen to cultured hepatocytes, the contents of reduced glutathione (GSH) and total glutathione decreased, the activity of glutathione peroxydase (GSH-Px) decreased, but the contents of oxidized glutathione (GSSG) showed no change. Pretreatment with NT before acetaminophen decreased the contents of GSH further but increased the contents of GSSG and total glutathione and enhanced the activity of GSH-Px. These results indicated that NT may enhance synthesis of glutathione and the ability for hepatocytes to scavenge free radicals by increasing the activity of GSH-Px accompanied by oxidation of GSH to GSSG.
Department of Physiology, Beijing Medical University.
J Y
JY
Li
L
L
Wang
X J
XJ
Zhang
D000082:Acetaminophen; D000818:Animals; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000517:prevention & control*; D004247:DNA / Q000096:biosynthesis; D016166:Free Radical Scavengers; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism*; D019803:Glutathione Disulfide; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D009496:Neurotensin / Q000494:pharmacology* / Q000627:therapeutic use
D016166:Free Radical Scavengers; D000082:Acetaminophen; D009496:Neurotensin; D004247:DNA; D005979:Glutathione Peroxidase; D005978:Glutathione; D019803:Glutathione Disulfide