High-performance liquid chromatographic assay for 2'-deoxy-3'-thiacytidine in human serum.
A high-performance liquid chromatographic method for the determination of 2'-deoxy-3'-thiacytidine (3TC), a novel dideoxy-nucleoside analogue, in human serum is described. 3TC was extracted from serum samples using Bond Elut Certify solid-phase extraction cartridges prior to reversed-phase chromatography with UV detection. The method has been shown to be valid over the concentration range 10-5000 ng/ml using a 1-ml sample volume, both before and after heat treatment of the samples at 60 degrees C for 3 h. The method has been automated using a Zymark robot and used in the analysis of serum samples from HIV positive patients.
Drug Metabolism Department, Glaxo Research and Development, Greenford, Middlesex, UK.
A J
AJ
Harker
G L
GL
Evans
A E
AE
Hawley
D M
DM
Morris
D000998:Antiviral Agents / Q000097:blood* / Q000493:pharmacokinetics; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D015224:Dideoxynucleosides / Q000097:blood; D006679:HIV Seropositivity / Q000097:blood; D006358:Hot Temperature; D006801:Humans; D019259:Lamivudine; D011786:Quality Control; D012680:Sensitivity and Specificity; D016047:Zalcitabine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
D000998:Antiviral Agents; D015224:Dideoxynucleosides; C057503:carbovir; D019259:Lamivudine; D016047:Zalcitabine