[Experimental gene transfer study using anucleated reticulocytes as target cells].
We report here a novel approach to introduce reporter beta-galactosidase genes into anucleated rabbit reticulocytes. The results indicate that the beta-galactosidase gene was expressed to certain degree in the cytoplasm of the reticulocytes. Our study has provided a useful model system for gene expression studies in anucleated eukaryotic cells.
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
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Ma
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Xue
D000818:Animals; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D008297:Male; D010957:Plasmids; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics*
D001616:beta-Galactosidase