Construction of recombinant Marek's disease virus type 1 (MDV1) expressing the Escherichia coli lacZ gene as a possible live vaccine vector: the US10 gene of MDV1 as a stable insertion site.
This paper describes the construction of a recombinant Marek's disease virus serotype 1 (MDV1) in which the Escherichia coli lacZ gene was inserted into the open reading frame homologous to the US10 gene of herpes simplex virus 1 (HSV1). The recombinant virus replicated as well in cell culture as the parental MDV1 K-554 strain. Chickens immunized with the virus were protected against challenge with virulent MDV1, and produced a high level of antibodies against beta-galactosidase as well as against MDV1 antigens. The antibody titres persisted for at least 16 weeks. These results demonstrate that the US10 gene of MDV1 is an effective site for the insertion of foreign genes from which to construct a polyvalent live vaccine for poultry.
Chemo-Sero Therapeutic Research Institute, Kikuchi Research Center, Kumamoto, Japan.
M
M
Sakaguchi
Y
Y
Hirayama
H
H
Maeda
K
K
Matsuo
M
M
Yamamoto
K
K
Hirai
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D008381:Herpesvirus 2, Gallid / Q000235:genetics* / Q000276:immunology; D007763:Lac Operon; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014765:Viral Vaccines