Involvement of androgen-induced growth factor (FGF-8) gene in mouse embryogenesis and morphogenesis.
Androgen-induced growth factor (AIGF/FGF-8) has purified from a mouse mammary carcinoma cell line and its cDNA cloning revealed that AIGF is the eighth member of the fibroblast growth factor family. To clarify a biological role of FGF-8, Northern blot analysis of various adult tissues was carried out, but no expression was found. However, whole mount in situ hybridization of 7.5- to 14.5-day gestation (E7.5-14.5) embryos revealed six predominant expression domains: [1]primitive streak region (E7.5-9.75); [2]midbrain-hindbrain border (E8.0-10.5); [3]rostral forebrain (E8.0-10.5); [4]limb ectoderm and apical ectodermal ridge (E9.25-13.5); [5]nasal placode and epithelium (E9.5-12.5); and [6]branchial arch ectoderm (E8.5-11.5). The embryonic expression pattern suggests a unique role of FGF-8 in mouse development, especially in gastrulation, brain development, and limb and facial morphogenesis.
Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, Japan.
H
H
Ohuchi
H
H
Yoshioka
A
A
Tanaka
Y
Y
Kawakami
T
T
Nohno
S
S
Noji
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000378:metabolism; D005121:Extremities / Q000196:embryology; D005145:Face / Q000196:embryology; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors; D018507:Gene Expression Regulation, Developmental; D006133:Growth Substances / Q000235:genetics*; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL / Q000196:embryology*; D009024:Morphogenesis; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
C496457:Fgf8 protein, mouse; D006133:Growth Substances; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors