[Band patterns of human sweat proteins carrying ABO-blood-group antigens tested by SDS-polyacrylamide gel electrophoresis].
Sweats were obtained from healthy 27 male and 5 female adults (A.Se: 11, A.se:1, B.Sc:5, B.Se:5, AB.Se:2 and O.Se:8 cases) in a sauna. Human sweat proteins carrying ABO-blood-group antigens were examined by SDS-polyacrylamide gel electrophoresis followed by immunoblotting with monoclonal anti-A and anti-B antibodies. Electrophoretic bands of the sweats were generally demonstrated as follows: A band in the region of a higher molecular weight than 116,000 Da, a band in each of the regions of 96,000, 82,000, 71,000, 50,000, 43,000 and from 34,000 to 30,000 Da, and several bands (2-6 bands) in region from 28,000 to 19,000 Da. There were many protein fractions carrying ABO-blood-group antigens in the sweats, regardless of the blood groups. Individual variations of the band patterns in the number and the color depth of immunoblotting were observed. The band in the sweats from the nonsecretors did not appear clearly. A band in the region between 34,000 and 30,000 Da, and several bands in the region between 28,000 and 19,000 Da were stained with PAS. These bands are probably glycoproteins with a blood group activity in sweats. Proteins carrying ABO-blood-group antigens in human erythrocyte membranes and saliva were also tested. In erythrocyte membranes, many bands ranged widely from high to low molecular weight regions were demonstrated, and several bands with higher molecular weights than 43,000 Da were shown in saliva. The band patterns of the sweats, erythrocyte membranes and saliva differed from one another, therefore, it was considered that there are proper proteins carrying ABO-blood-group antigens in sweats, erythrocyte membranes and saliva.
Department of Legal Medicine, School of Medicine, Iwate Medical University, Morioka, Japan.
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D000017:ABO Blood-Group System / Q000276:immunology*; D002352:Carrier Proteins / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007519:Isoantigens / Q000302:isolation & purification*; D008297:Male; D008970:Molecular Weight; D013542:Sweat / Q000737:chemistry*
D000017:ABO Blood-Group System; D002352:Carrier Proteins; D007519:Isoantigens