Determination of trace impurities of peptides and alkaloids by capillary electrophoresis-ion spray mass spectrometry.
Two different mixtures have been analyzed by CE-UV-MS using selected ion monitoring (SIM) conditions to evaluate whether this technique can detect trace impurities in such mixtures. The first mixture consisted of two bioactive peptide analogues which included Lys-bradykinin (kallidin) and Met-Lys-bradykinin. The presence of 0.1% Lys-bradykinin was detected by SIM CE-MS but not by CE-UV at the 0.1% level as it migrated from the capillary column prior to the major component, Met-Lys-bradykinin. The second mixture consisted of two antibacterial alkaloids, berberine and palmatine. The presence of 0.15% palmatine was detected by CE-UV and SIM CE-MS at the 0.15% level as it migrated from the capillary column following the major component, berberine. These results suggest that SIM CE-MS offers the necessary separation efficiencies and sensitivity to provide a complementary analytical determination of trace components in such sample mixtures.
Diagnostic Laboratory, Cornell University, Ithaca, NY 14850.
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Hsieh
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Cai
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Henion
D000470:Alkaloids / Q000737:chemistry*; D000595:Amino Acid Sequence; D001599:Berberine / Q000737:chemistry; D001600:Berberine Alkaloids / Q000737:chemistry; D001920:Bradykinin / Q000031:analogs & derivatives / Q000737:chemistry; D004340:Drug Contamination; D004586:Electrophoresis / Q000379:methods*; D007609:Kallidin / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000470:Alkaloids; D001600:Berberine Alkaloids; D009842:Oligopeptides; D001599:Berberine; D007609:Kallidin; C018103:bradykinin, Met-Lys-; C005413:palmatine; D001920:Bradykinin