Effect of template size on accumulation of defective interfering RNAs in protoplasts.
A turnip protoplast system has been used to study the effects of template size and sequence on the replication and/or stability of a small defective interfering (DI) RNA associated with turnip crinkle virus. Our results indicated that as little as a single base difference in the size of the molecule in some regions, rather than the specific sequence, affected the level of DI RNA accumulating in protoplasts.
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Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003.
Simon
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D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis; D003673:Defective Viruses / Q000502:physiology*; D016296:Mutagenesis; D010942:Plant Viruses / Q000502:physiology*; D011523:Protoplasts / Q000821:virology; D012367:RNA, Viral / Q000096:biosynthesis*; D017384:Sequence Deletion; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014675:Vegetables / Q000821:virology*; D014779:Virus Replication
D004279:DNA, Viral; D012367:RNA, Viral