An investigation of the antitumor peptidoglycan fraction from squid ink.
Squid ink, which has little commercial use and is usually discarded, was extracted using a Tris-HCl buffer (pH 6.8). The extract was fractionated using DEAE Sephacel ion-exchange chromatography and Sephacryl S-300 gel filtration to give a peptidoglycan fraction which exhibited strong antitumor activity against Meth-A fibrosarcoma in BALB/c mice following intraperitoneal administration. The fraction was composed of 7.8% peptide, 57% polysaccharide and 30% pigment. The polysaccharide component had a unique structure with equimolar ratios of GlcA, GalNAc and Fuc. Since the fraction has no direct cytotoxic effect on Meth-A cells, inhibition of tumor growth may be due to stimulation of host-mediated responses.
Aomori Advanced Industrial Technology Center, Japan.
Y
Y
Takaya
H
H
Uchisawa
H
H
Matsue
B
B
Okuzaki
F
F
Narumi
J
J
Sasaki
K
K
Ishida
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D002455:Cell Division / Q000187:drug effects; D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange; D049832:Decapodiformes / Q000378:metabolism*; D005260:Female; D005354:Fibrosarcoma / Q000188:drug therapy*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D010457:Peptidoglycan / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D011402:Pronase / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D010457:Peptidoglycan; D011402:Pronase