Neonatal guanethidine treatment alters endoneurial but not dorsal root ganglion perfusion in the rat.
In previous work, we suggested that there were differences in vasoregulation between dorsal root ganglia (DRG) and the endoneurium of peripheral nerve trunks. To investigate sympathetic control of both microvessel beds, we compared local perfusion in the sciatic nerve endoneurium and lumbar DRG of adult Sprague-Dawley rats treated from neonatal day 5 with guanethidine monosulfate to induce adrenergic sympathectomy. Control rats were injected with normal saline. Local blood flow and microvascular resistance were measured using microelectrodes sensitive to the clearance of hydrogen. Guanethidine-sympathectomized rats had higher sciatic endoneurial blood flow and lower endoneurial microvascular resistance than saline-injected controls. In contrast, DRG blood flow was not increased by sympathectomy and was comparable to control values despite the hypotension induced by sympathectomy. The results suggest that sympathetic control of local blood flow and may be less apparent in DRG than endoneurium and that local autoregulation may protect DRG from hypotension.
Department of Clinical Neurosciences, University of Calgary, Alta, Canada.
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D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001794:Blood Pressure / Q000187:drug effects; D005727:Ganglia, Spinal / Q000098:blood supply* / Q000187:drug effects; D006145:Guanethidine / Q000494:pharmacology*; D008297:Male; D010525:Peripheral Nerves / Q000098:blood supply* / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects; D012584:Sciatic Nerve / Q000098:blood supply; D013563:Sympathectomy, Chemical; D014655:Vascular Resistance / Q000187:drug effects; D015430:Weight Gain / Q000187:drug effects
D006145:Guanethidine