Monoclonal antibodies against goldfish (Carassius auratus) immunoglobulin: application to the quantification of immunoglobulin and antibody-secreting cells by ELISPOT and seric immunoglobulin and antibody levels by ELISA in carp (Cyprinus carpio).
Monoclonal antibodies (mAbs) raised against heavy and light chains of goldfish immunoglobulin (Ig) were characterized by a Western blot technique. A complete cross-reactivity was observed between carp and goldfish Ig. These mAbs were used for the quantification of carp Ig and anti-Yersinia ruckeri antibodies by ELISA. An ELISPOT assay was also developed in carp to quantify Ig-secreting cells (ISC) and antibody-secreting cells (ASC). The number of ASC was maximum on day 18 post-vaccination and decreased to the basal level on day 28. The antibody levels in sera were maximum on day 18 and slowly decreased until day 28.
Inland Fisheries Institute, Ichtyopathology and Immunology Laboratory, Piaseczno, Poland.
A K
AK
Siwicki
C
C
Vergnet
J
J
Charlemagne
M
M
Dunier
D000818:Animals; D000906:Antibodies / Q000097:blood; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006054:Goldfish / Q000276:immunology*; D006825:Hybridomas; D007118:Immunoassay / Q000662:veterinary; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007136:Immunoglobulins / Q000097:blood / Q000276:immunology*; D007700:Kinetics; D007958:Leukocyte Count / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination / Q000662:veterinary
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins