Effects of thymus supernatants on the phenotype of different subsets of peritoneum antigen-presenting cells.
Peritoneal cells (PC) obtained 2 h subsequent to intraperitoneal (i.p.) injection of low doses (200 micrograms) of a purified fraction of rat male accessory glands (FI-RAG) are phenotypically and functionally different from those obtained 24 h after i.p. injection. In fast, PC obtained 2 h after FI-RAG injection are mainly IE+ and are involved in inducing specific suppression to RAG. In contrast, PC obtained 24 h after FI-RAG injection are mainly IA+ and capable of inducing specific response to RAG. For their induction, IA+ PC require cells within or recently derived from bone marrow. In order to analyze the mechanisms involved in IA+ bone marrow-dependent cell generation in the peritoneum, we studied the distribution of FI-RAG following i.p. injection. It was established that FI-RAG is found mainly in the thymus 2 h after injection and remains there for at least 24 h. Subsequently, we analyzed, in 4 groups of rats, the influence of thymic culture supernatants on the phenotype of cells appearing in the peritoneal cavity 2 h after FI-RAG injection. An increase in IA+ PC was observed 2 h after i.p. injection of FI-RAG in animals that had received either supernatants from normal thymic cells cultured with FI-RAG or those from thymic cells taken from animals injected with FI-RAG 2 h prior to being killed. Supernatants of thymic cells from animals injected with FI-RAG 24 h prior to being killed or from normal thymic cells do not increase the percentage of IA+ PC.(ABSTRACT TRUNCATED AT 250 WORDS)
Departamento de Bioquímica Clinica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
M
M
Maccioni
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M
Romero-Piffiguer
V E
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Rivero
C M
CM
Riera
M E
ME
Ferro
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis; D001324:Autoantigens / Q000276:immunology; D015551:Autoimmunity; D005837:Genitalia, Male / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D016130:Immunophenotyping; D007274:Injections, Intraperitoneal; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000276:immunology*
D000954:Antigens, Surface; D001324:Autoantigens; D000949:Histocompatibility Antigens Class II