Effects of vitamin E deficiency on hepatic microsomal cytochrome P450 and phase II enzymes in male and female rats.
The effects of a vitamin E (VE) deficient diet given for 120 days on total cytochrome P450 (P450) contents and UDP-glucuronyl transferase (UDPGT) and sulfotransferase (ST) activities were investigated in rat liver of both sexes. The VE deficient regimen lowered the serum VE levels, increasing the hepatic malondialdehyde concentration regardless of animal sex, which shows an elevation of the tissue peroxi-disability. Hepatic total P450 contents were significantly decreased in male rats given the VE deficient diet, whereas the effects on female rats were less obvious. Concerning phase II enzymes, VE deficiency had no effect on the activities of UDPGT and ST in rats of both sexes. The data suggest that overall capacity of detoxification of xenobiotics may be impaired by VE deficiency to a much greater extent in male than in female rat liver.
Dept. of Nutrition, Tokyo University of Agriculture, Japan.
M
M
Iwasaki
M
M
Iwama
N
N
Miyata
Y
Y
Iitoi
Y
Y
Kanke
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005260:Female; D014453:Glucuronosyltransferase / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D015238:Sulfotransferases / Q000378:metabolism*; D014810:Vitamin E / Q000097:blood; D014811:Vitamin E Deficiency / Q000201:enzymology*
D014810:Vitamin E; D003577:Cytochrome P-450 Enzyme System; D014453:Glucuronosyltransferase; D015238:Sulfotransferases