Induction and immunological characterization of the uridine diphosphate-glucuronosyltransferase conjugating 1-naphthol in the rat choroid plexus.
The uridine diphosphate-glucuronosyltransferase isoform conjugating 1-naphthol has a very high activity in the rat choroid plexus. We showed that antibodies raised against the main liver isoenzyme cross-reacted with a choroidal protein exhibiting the same molecular weight as the hepatic form. Both enzymes had a similar affinity for 1-naphthol. After an in vivo treatment by 3-methylcholanthrene, a 3-fold increase of the activity in both the liver and choroid plexuses was observed. These results suggest that the choroidal and hepatic enzymes conjugating 1-naphthol are identical. This high metabolic activity suggests a metabolic protection of the brain by the choroid plexus.
Centre du Médicament, CNRS URA 597, Nancy, France.
B
B
Leininger-Muller
J F
JF
Ghersi-Egea
G
G
Siest
A
A
Minn
D000818:Animals; D000906:Antibodies; D002831:Choroid Plexus / Q000187:drug effects / Q000201:enzymology*; D004790:Enzyme Induction; D014453:Glucuronosyltransferase / Q000032:analysis / Q000096:biosynthesis*; D015151:Immunoblotting; D007527:Isoenzymes / Q000032:analysis / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008970:Molecular Weight; D009284:Naphthols / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013379:Substrate Specificity
D000906:Antibodies; D007527:Isoenzymes; D009284:Naphthols; C029350:1-naphthol; D008748:Methylcholanthrene; D014453:Glucuronosyltransferase