Distribution of cytoskeletal proteins (neurofilaments, peripherin and MAP-tau) in the cochlea of the human fetus.
We report here an immunohistochemical study of the distribution of intermediate filaments (neurofilament, peripherin) and a microtubule-associated protein, tau, in the human fetal cochlea at 27 weeks of gestation. Neurofilament immunoreactivity (160 and 200 KDa) was localized in afferent and efferent fibers of the cochlear innervation and restricted to a few small spiral ganglion neurons. Peripherin immunoreactivity was specifically distributed in some small ganglion neurons and in their central and peripheral extensions, particularly in fibers reaching the lower part of the outer hair cells. Double immuno-labelling studies with these neurofilaments and peripherin antibodies show that only small neuron cell bodies were stained. Morpholometrical data indicate that immunostained neurons could be related to the Type II neuron population in the spiral ganglion. Tau protein was localized in intraganglionic spiral bundle fibers and in fibers that reach the lower part of hair cells. These observations suggest that neurofilament and peripherin antibodies stain a particular population of human spiral ganglion neurons with Type II characteristics. Moreover, the specificity of peripherin labelling in Type II cells and their processes suggest that peripherin could be used as a probe for the developmental study of this system in the human cochlea. On the other hand, tau antibody appeared as a marker for efferent fibers during development and could give information on the ontogenesis of efferent innervation.
Laboratoire de Neurobiologie et Physiologie du Développement, Université Blaise Pascal, Aubière, France.
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Després
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Leger
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Dahl
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Romand
D003051:Cochlea / Q000196:embryology / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D005333:Fetus; D006198:Hair Cells, Auditory / Q000196:embryology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008562:Membrane Glycoproteins; D009412:Nerve Fibers / Q000378:metabolism / Q000648:ultrastructure; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism / Q000648:ultrastructure; D009476:Neurons, Efferent / Q000378:metabolism / Q000648:ultrastructure; D009479:Neuropeptides / Q000378:metabolism*; D009925:Organ of Corti / Q000196:embryology / Q000378:metabolism; D064531:Peripherins; D013136:Spiral Ganglion / Q000196:embryology / Q000378:metabolism; D000159:Vestibulocochlear Nerve / Q000196:embryology / Q000378:metabolism; D016875:tau Proteins / Q000378:metabolism*
D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D009479:Neuropeptides; C578994:PRPH protein, human; D064531:Peripherins; D016875:tau Proteins