[Effect of danazol on growth of endometrial and endometriotic cells in vitro].
The cells from entopic and ectopic endometrial tissues were primarily cultured in collagen gel and passaged for secondary culture. The grown cells were treated respectively with danazol (2.5 micrograms/ml and 5 micrograms/ml) and without danazol in medium. In control culture without danazol, the cells presented normal growth, had stellate appearance and were connected with each other by cytoplasmic projections. Cell growth was suppressed after being treated with both concentrations of danazol. Attachment potential of passaged cells in 24 hours appeared to be damaged, some cells revealed lethal pathological features. Ultrastructural changes induced by danazol included numerous vacuoles and lipid droplets, increased lysosomes including autolysosome, oncotic mitochondria in cytoplasm, irregular nucleus, etc. The results suggest that collagen gel culture is suitable for culturing endometrial cells, and danazol has a direct effect on human endometrial and endometriotic tissues.
Women's Hospital, Zhejiang Medical University, Hangzhou.
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Xie
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Stevenson
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Kunstmann
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D003613:Danazol / Q000494:pharmacology*; D004715:Endometriosis / Q000473:pathology*; D004717:Endometrium / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D008247:Lysosomes / Q000187:drug effects; D008933:Mitochondrial Swelling / Q000187:drug effects; D014591:Uterine Diseases / Q000473:pathology*
D003470:Culture Media; D003094:Collagen; D003613:Danazol