Stimulation of macrophage oxygen free radical production and lymphocyte blastogenic response by immunization with porins.
Porins were prepared from smooth strain of Salmonella typhi 0-901 and chemotype rough mutant of S. typhimurium Ra-30. Porins could significantly stimulate the immune systems of mice. Immunization of mice with the porins provoked synthesis of anti-porin antibodies. Macrophages from the immunized mice showed increased capacity to generate oxygen free radicals, and lymphocytes from these mice showed proliferative response to the porins. Thus porins may play a role in providing protection from salmonellosis by stimulating the antibody production and increasing the capacity of macrophages to generate oxygen free radicals along with stimulation of lymphocytes.
Department of Experimental Medicine, Postgraduate Institute of Medical Education & Research, Chandigarh, India.
B
B
Tabaraie
B K
BK
Sharma
P R
PR
Sharma
R
R
Sehgal
N K
NK
Ganguly
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D008163:Luminescent Measurements; D008213:Lymphocyte Activation; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D010100:Oxygen / Q000378:metabolism; D018272:Porins / Q000276:immunology*; D012475:Salmonella / Q000276:immunology*; D012485:Salmonella typhi / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology; D014611:Vaccination
D000907:Antibodies, Bacterial; D005609:Free Radicals; D018272:Porins; D010100:Oxygen