Endothelial seeding of compliant polyurethane vascular graft material.
Compliance was measured in a new compliant polyurethane vascular graft material and in polytetrafluoroethylene (PTFE) graft material using an ultrasonic device; attachment of indium-111 oxine-labelled human endothelial cells to both surfaces with a range of surface coatings was assessed. Compliant polyurethane was six to eight times more compliant than PTFE (P < 0.01) at all pressures in the range 50-120 mmHg, and endothelial cell attachment to uncoated polyurethane was three times better than to uncoated PTFE at times up to 90 min (P < 0.01). Attachment to polyurethane was also better after blood clot, collagen and fibronectin treatment at times up to 30 min (P < 0.05). Endothelial seeding of compliant graft material may provide a prosthetic vascular substitute with characteristics similar to those of autologous vein.
University Department of Surgery, Royal Free Hospital and School of Medicine, London, UK.
G
G
Stansby
C
C
Berwanger
N
N
Shukla
T
T
Schmitz-Rixen
G
G
Hamilton
D000368:Aged; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011138:Polytetrafluoroethylene; D011140:Polyurethanes; D011312:Pressure; D014471:Umbilical Veins / Q000502:physiology; D014654:Vascular Patency
D011140:Polyurethanes; D011138:Polytetrafluoroethylene