Nitric oxide mediates the amplification by interleukin-1 beta of neurogenic vasodilatation in the rat skin.
Blood flow in the plantar hindpaw skin of anaesthetized rats was measured by laser Doppler flowmetry. Intraplantar injection of interleukin-1 beta (50 pg) significantly enhanced the hyperaemic response to intraplantar capsaicin (0.3 microgram). Pretreatment with a neurotoxic dose of capsaicin reduced the capsaicin-evoked hyperaemia and prevented the facilitatory effect of interleukin-1 beta. Blockade of nitric oxide formation by NG-nitro-L-arginine methyl ester failed to affect the capsaicin-evoked vasodilatation but abolished its amplification by interleukin-1 beta. These data indicate that the enhancement by interleukin-1 beta of the capsaicin-induced hyperaemia involves thin afferent nerve fibres and depends on nitric oxide as essential intermediate.
Department of Anaesthesiology, University of Würzburg, Germany.
M K
MK
Herbert
P
P
Holzer
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D019331:NG-Nitroarginine Methyl Ester; D009475:Neurons, Afferent / Q000187:drug effects; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects; D012867:Skin / Q000098:blood supply* / Q000294:innervation / Q000378:metabolism; D014664:Vasodilation / Q000187:drug effects*
D007375:Interleukin-1; D009569:Nitric Oxide; D001120:Arginine; D002211:Capsaicin; D019331:NG-Nitroarginine Methyl Ester