Comparison of two rapid enzyme immunoassays with standard enzyme immunoassay and latex agglutination for the detection of human rotavirus in stools.
We evaluated the performance of six commercially available immunoassays for their ability to detect rotaviruses in stool specimens. Four of the assays were enzyme-linked immunosorbent assays (ELISA), including two rapid enzyme immunoassays (Testpack, Abbott Laboratories, Chicago and Pinpoint, Cambridge Biomedical, UK), and the remaining two were latex agglutination tests. A total of 100 specimens from children with gastroenteritis were tested with the commercial immunoassays and compared with electron microscopy (EM) and polyacrylamide gel electrophoresis (PAGE) as reference tests for the detection of rotavirus. Discordant results were further evaluated by a standard blocking ELISA. The rapid EIAs were found to be highly sensitive (100% for the Testpack and 97% for the Pinpoint) and more sensitive than the other immunoassays tested. High specificity was also recorded with both rapid EIAs (96% and 100% for Testpack and Pinpoint, respectively). The rapid EIA tests were easy to perform, required no specialized equipment, and could yield a result in less than 15 min. The results show that each of the commercial assays evaluated could accurately detect rotavirus in the stools of children with gastroenteritis, although the suitability of choice of assay will ultimately depend upon the requirements of the individual laboratory.
Department of Virology, Medical University of Southern Africa, Medunsa.
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Steele
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Williams
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Bos
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Peenze
D005243:Feces / Q000382:microbiology*; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007841:Latex Fixation Tests; D012401:Rotavirus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors