Cytotoxic activity against maedi-visna virus-infected macrophages.
The cell type predominantly infected by maedi-visna virus (MVV) is the macrophage, and we have looked at the ability of MVV-infected macrophages to interact with cytotoxic T lymphocytes (CTL), important effector cells against virus infections. MVV-specific CTL precursors were detected, after in vitro culture with MVV antigen and recombinant human interleukin-2, in peripheral blood lymphocytes of all MVV-infected sheep. MVV-infected monocyte-derived macrophages and alveolar macrophages were able to stimulate CTL activity in vitro and were targets for these activated CTL. The major effector cell population using MVV-infected macrophage targets was CD8+ lymphocytes, although another population, lymphokine-activated killer cells, may also have been involved. There was no direct cytotoxic activity found in alveolar lymphocytes from MVV-infected sheep without lung lesions.
Department of Veterinary Pathology, University of Edinburgh, Summerhall, United Kingdom.
W C
WC
Lee
I
I
McConnell
B A
BA
Blacklaws
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005347:Fibroblasts / Q000276:immunology / Q000821:virology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000276:immunology / Q000821:virology; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000821:virology*; D016676:Macrophages, Alveolar / Q000276:immunology / Q000821:virology; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D012756:Sheep; D012867:Skin; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology*; D014790:Visna-maedi virus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007376:Interleukin-2; D011994:Recombinant Proteins