[A simplified method for preparation of DOCA-salt hypertension model in rats by subcutaneous implantation of DOCA silastic tube].
To study a simplified method for preparation of DOCA-Salt hypertension model in rat. 45 male SD rats (140 +/- 9 g) were used in this study. A silastic tube (length 25 mm; external diameter 4 mm; internal diameter 2.5 mm; 10-14 about 300 microns micropores on the wall) filled 100 mg DOCA in was implanted subcutaneously at the belly of the rat and followed by uninephrectomy. After operation the rat only received 1% salt solution in stead of drinking water. Hypertension developed within 3 weeks. At the 8th week after operation, systolic blood pressure (SBP) of rats was elevated to 23.3 +/- 0.37 kPa. If DOCA (10 mg/rat, weekly) was given subcutaneously, however, from control level of 16 +/- 0.16 kPa hypertension did not occur until the 5th week after injection. At the 13th week, SBP was elevated to 23.3 +/- 0.66 kPa. Difference of regression coefficients (1.295, 0.692) of SBP curves between two experimental groups was very significant (P < 0.001). Compared with subcutaneous injection, there are two advantages for method of subcutaneous implantation: (1) The rate of SBP elevation is faster and amplitude higher. (2) This method is simple and well reproducible.
Department of Physiology, Guangzhou Medical College.
Q
Q
Wang
Z
Z
Chen
X P
XP
Fan
D H
DH
Xu
G H
GH
Zhou
Z X
ZX
Liang
D000818:Animals; D003900:Desoxycorticosterone; D004195:Disease Models, Animal; D004343:Drug Implants; D006973:Hypertension / Q000209:etiology*; D008297:Male; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D012826:Silicone Elastomers
D004343:Drug Implants; D012826:Silicone Elastomers; D003900:Desoxycorticosterone