The oxygenase reaction of acetolactate synthase detected by chemiluminescence.
In addition to the synthesis of ketolacids the enzyme acetolactate synthase shows an oxygen-consuming side reaction. Partially purified acetolactate synthase from corn (Zea mays L.) and barley (Hordeum vulgare L.) exhibits chemiluminescence in the presence of oxygen, Mn2+ and low concentrations of pyruvate. Light emission is inhibited by azide, but not by catalse or superoxide dismutase. The data suggest the formation of singlet oxygen during the catalytic cycle, and provides a basis for a highly sensitive assay for the oxygenase reaction of acetolactate synthesis. Both synthase activity and chemiluminescence are inhibited by sulfonylurea herbicides. The results add a new aspect to the irreversible inhibition of acetolactate synthase by these herbicides which may be enhanced by the presence of reactive oxygen species.
Lehrstuhl für Physiologie und Biochemie, Pflanzen Universität Konstanz, Germany.
J
J
Durner
V
V
Gailus
P
P
Böger
D000095:Acetolactate Synthase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D006540:Herbicides / Q000494:pharmacology; D001467:Hordeum / Q000201:enzymology*; D008163:Luminescent Measurements; D010105:Oxygenases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012588:Scintillation Counting; D013449:Sulfonamides; D014227:Triazines / Q000494:pharmacology; D003313:Zea mays / Q000201:enzymology*
D006540:Herbicides; D017382:Reactive Oxygen Species; D013449:Sulfonamides; D014227:Triazines; D010105:Oxygenases; D000095:Acetolactate Synthase; C037137:chlorsulfuron