[Pyoverdin from Pseudomonas fluorescens: binding to the globulin fraction of serum and its effect on serological reaction titers].
Pseudomonas fluorescens grows and produces pigment in refrigerated human blood at 4 degrees C. Saline precipitation of plasma showed that globulin and albumin fractions retained pyoverdin at different concentrations. By dialysis it was possible to determine that the pigment attached or aggregated to the protein in total plasma as well as in the fraction obtained by saline precipitation. A greater binding was observed at the globulin fraction. Gamma-globulin immunological activity was reduced due to the interaction with pyoverdin, as much in agglutination reaction (VDRL) as in neutralization (Streptolysin O).
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
M C
MC
Pájaro
I L
IL
Barberis
S D
SD
Godino
I
I
Albesa
D000372:Agglutination Tests; D000989:Antistreptolysin / Q000097:blood*; D001667:Binding, Competitive; D001798:Blood Proteins / Q000378:metabolism*; D002308:Cardiolipins / Q000097:blood*; D002784:Cholesterol / Q000097:blood*; D005188:False Negative Reactions; D005453:Fluorescence; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D009842:Oligopeptides; D010713:Phosphatidylcholines / Q000097:blood*; D010860:Pigments, Biological / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011551:Pseudomonas fluorescens / Q000737:chemistry*; D013588:Syphilis Serodiagnosis
D001798:Blood Proteins; D002308:Cardiolipins; D007136:Immunoglobulins; D009842:Oligopeptides; D010713:Phosphatidylcholines; D010860:Pigments, Biological; C054448:VDRL antigen; C042453:pyoverdin; D000989:Antistreptolysin; D002784:Cholesterol
Pioverdina de Pseudomonas fluorescens: unión a la fracción globulina del suero y efecto sobre los títulos de reacciones serólogicas.