Chemical analysis of neurotransmitter candidates in clonal cell lines from Drosophila central nervous system. I. ACh and L-dopa.
To characterize neuronal phenotypes, aminergic neurotransmitters, i.e. acetylcholine (ACh), catecholamine (CA)s and other biogenic amines (serotonin, octopamine), were surveyed in 10 colonial clones from one cell line of Drosophila larval CNS using HPLC-ECD system. ACh, which is a neurotransmitter in Drosophila, was found in 7 out of 10 clones. CAs, their metabolites, and other amines were not detected in any clones. However, all the 10 clones expressed L-dopa, a precursor of CAs. Consequently, seven clones expressed ACh and L-dopa. L-dopa as a novel neurotransmitter candidate was discussed.
Department of Pharmacology, Nippon Medical School, Tokyo, Japan.
K
K
Ui-Tei
S
S
Nishihara
M
M
Sakuma
K
K
Matsuda
T
T
Miyake
Y
Y
Miyata
D000109:Acetylcholine / Q000032:analysis*; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism; D002395:Catecholamines / Q000032:analysis / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002999:Clone Cells; D003010:Clorgyline / Q000494:pharmacology; D004330:Drosophila / Q000378:metabolism*; D004563:Electrochemistry; D007980:Levodopa / Q000032:analysis*; D018377:Neurotransmitter Agents / Q000032:analysis*
D015306:Biogenic Monoamines; D002395:Catecholamines; D018377:Neurotransmitter Agents; D007980:Levodopa; D003010:Clorgyline; D000109:Acetylcholine