Protein synthesis is required for the generation of oscillatory current responses in Xenopus oocyte.
In Xenopus oocyte, direct activation of G-proteins by AIF4- or injection of inositol 1, 4, 5-trisphosphate evoked Ca(2+)-gated Cl-current responses. The current responses were smooth and not oscillatory when tested immediately after the oocyte was isolated. After hours of incubation, the response became oscillatory. This change was prevented by cycloheximide, a protein synthesis inhibitor. Results indicate that the characteristics of Ca2+ signaling system have changed from not oscillatory to oscillatory with time and protein synthesis was required for this change.
Department of Physiology, Kobe University School of Medicine, Japan.
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Nakamachi
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Ando
D000109:Acetylcholine / Q000494:pharmacology; D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D018118:Chloride Channels / Q000502:physiology*; D005459:Fluorides / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism; D015640:Ion Channel Gating; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D014176:Protein Biosynthesis; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism; D014982:Xenopus laevis
D017607:Aluminum Compounds; D018118:Chloride Channels; D011976:Receptors, Muscarinic; D019204:GTP-Binding Proteins; D000109:Acetylcholine; D005459:Fluorides; D002118:Calcium; C032311:aluminum fluoride