Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination.
We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.
Tsinoremas
N F
NF
Department of Biology, Texas A&M University, College Station 77843-3258.
Kutach
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AK
Strayer
C A
CA
Golden
S S
SS
D000595:Amino Acid Sequence; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D000458:Cyanobacteria / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D006639:Histidine / Q000096:biosynthesis; D008969:Molecular Sequence Data; D014170:Transformation, Genetic
D006639:Histidine