Characterization of human B cell proteins binding specifically to uveitopathogenic peptide 1169-1191 of bovine IRBP.
Peptide 1169-1191 is a major uveitopathogenic determinant of bovine Interphotoreceptor Retinoid Binding Protein (IRBP) in Lewis rats. Previously, we identified two proteins with approximate molecular masses of 72 and 74 kDa and one with a molecular mass of 40 kDa from B cells of naive Lewis rats and EBV-transformed B cells from a human patient with ocular Behçet's disease that bind to bovine IRBP peptide 1169-1191. In this study, we have partially characterized these proteins. The two proteins with molecular masses 72 and 74 kDa belong to the HSP 70 family of proteins and the 40-kDa protein is actin.
Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, MD 20892.
K
K
Rengarajan
M D
MD
de Smet
G J
GJ
Chader
B
B
Wiggert
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002417:Cattle; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011917:Rats, Inbred Lew; D012177:Retinol-Binding Proteins / Q000378:metabolism*; D000099214:Retinol-Binding Proteins, Interstitial
D000255:Adenosine Triphosphate; D001798:Blood Proteins; D005136:Eye Proteins; D010446:Peptide Fragments; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial