Fluorescence of matrix isolated guanine and 7-methylguanine.
We have prepared argon and nitrogen matrices containing guanine and 7-methylguanine, and measured their absorption, fluorescence excitation and fluorescence emission spectra. The fluorescence excitation spectrum of guanine shows four well-resolved bands in the range from 170 to 290 nm; excitation at the wavelengths of each of these bands results in a fluorescence emission with maximum intensity near 350 nm and a single-exponential decay with a lifetime of about 10 ns. There are significant differences between the fluorescent excitation and emission spectra of guanine and of 7-methylguanine, suggesting that the fluorescence observed from the guanine sample does not arise from a minority tautomer.
Polewski
K
K
Biology Department, Brookhaven National Laboratory, Upton, NY 11973.
Zinger
D
D
Trunk
J
J
Monteleone
D C
DC
Sutherland
J C
JC
D001128:Argon; D002942:Circular Dichroism; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry*; D008968:Molecular Conformation; D009584:Nitrogen; D013050:Spectrometry, Fluorescence / Q000379:methods
D006147:Guanine; C008450:7-methylguanine; D001128:Argon; D009584:Nitrogen