Potency and selectivity of inhibition of human immunodeficiency virus protease by a small nonpeptide cyclic urea, DMP 323.
DMP 323 is a potent inhibitor of the protease of human immunodeficiency virus (HIV), with antiviral activity against both HIV type 1 and HIV type 2. This compound is representative of a class of small, novel, nonpeptide cyclic urea inhibitors of HIV protease that were designed on the basis of three-dimensional structural information and three-dimensional database searching. We report here studies of the kinetics of DMP 323 inhibition of the cleavage of peptide and HIV-1 gag polyprotein substrates. DMP 323 acts as a rapidly binding, competitive inhibitor of HIV protease. DMP 323 is as potent against both peptide and viral polyprotein substrates as A-80987, Q8024, and Ro-31-8959, which are among the most potent inhibitors of HIV protease described in the literature to date. Incubation with human plasma or serum did not decrease the effective potency of DMP 323 for HIV protease, suggesting that plasma protein binding is of a low affinity relative to that of HIV protease. DMP 323 was also assessed for its ability to inhibit the mammalian proteases renin, pepsin, cathepsin D, cathepsin G, and chymotrypsin. No inhibition of greater than 12% was observed for any of these enzymes at concentrations of DMP 323 that were 350 to 40,000 times higher than that required to inhibit the viral protease 50%.
Erickson-Viitanen
S
S
Molecular Biology Department, DuPont Merck Pharmaceutical Company, Wilmington, Delaware 19880-0400.
Klabe
R M
RM
Cawood
P G
PG
O'Neal
P L
PL
Meek
J L
JL
D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors; D001381:Azepines; D001769:Blood; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D016333:HIV Protease / Q000032:analysis / Q000302:isolation & purification; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000201:enzymology; D015498:HIV-2 / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D014508:Urea / Q000031:analogs & derivatives* / Q000494:pharmacology
D001381:Azepines; D017320:HIV Protease Inhibitors; D014508:Urea; D002918:Chymotrypsin; D016282:Aspartic Acid Endopeptidases; D016333:HIV Protease; C085816:DMP 323