Sensitive high-performance liquid chromatographic method for the determination of the three main metabolites of selegiline (L-deprenyl) in human plasma.
A high-performance liquid chromatographic (HPLC) method with fluorescence detection was developed for the determination in human plasma of the three main metabolites of selegiline (L-deprenyl): amphetamine, methamphetamine and norselegiline. The HPLC separation of the analytes was performed under isocratic conditions, after extraction from plasma and precolumn derivatization with 9-fluorenylmethyl chloroformate. The linearity, precision and accuracy of the method were evaluated; the limit of quantification for all three metabolites in plasma was 0.5 ng/ml.
Pharmacia-Farmitalia Carlo Erba, R&D, Pharmacokinetics and Metabolism Department, Milan, Italy.
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La Croix
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Pianezzola
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M
Strolin Benedetti
D000661:Amphetamine / Q000097:blood; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes; D006801:Humans; D007202:Indicators and Reagents; D008694:Methamphetamine / Q000097:blood; D011786:Quality Control; D012642:Selegiline / Q000097:blood* / Q000493:pharmacokinetics
D005449:Fluorenes; D007202:Indicators and Reagents; D012642:Selegiline; D008694:Methamphetamine; C054007:1-(9-fluorenyl)methyl chloroformate; D000661:Amphetamine