Antibodies directed against alpha subunits of Gi, Gx/z, GO and GS transducer proteins reduced the morphine withdrawal syndrome in mice.
The effect of intracerebroventricular (i.c.v.) injection of antibodies directed against alpha subunits of Gi, Gx/z, GO and GS regulatory proteins on morphine dependence was analyzed in mice. Animals were rendered tolerant-dependent by subcutaneous (s.c.) implantation of an oily suspension (10 ml/kg) containing 0.1 g/ml of morphine. After 72 h of chronic morphine, 1 mg/kg s.c. naloxone precipitated the withdrawal syndrome. The anti Gi2 alpha, Gx/z alpha, GO1/2 alpha and GS alpha antibodies given 24 h before starting the chronic morphine treatment, reduced the number of jumps recorded. An effect also produced by pertussis toxin, agent impairing the function of Gi/GO transducer proteins. The antibodies injected 24 h before the naloxone challenge reduced the number of animals presenting the jumping behavior, as well as the average number of jumps. This was observed for antibodies against alpha subunits of Gi, Gx/z and GO1/2 proteins. Thus, i.c.v. injection of anti G alpha antibodies by reducing the function of opioid and non-opioid receptors alleviated the morphine withdrawal syndrome.
Instituto Cajal, C.S.I.C., Madrid, Spain.
P
P
Sánchez-Blázquez
J
J
Garzón
D000698:Analgesia; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D004361:Drug Tolerance; D019204:GTP-Binding Proteins / Q000276:immunology*; D007074:Immunoglobulin G / Q000008:administration & dosage*; D007276:Injections, Intraventricular; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D009020:Morphine / Q000009:adverse effects*; D009270:Naloxone / Q000008:administration & dosage; D037342:Pertussis Toxin; D013375:Substance Withdrawal Syndrome / Q000517:prevention & control*; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000008:administration & dosage
D000906:Antibodies; D007074:Immunoglobulin G; D010566:Virulence Factors, Bordetella; D009270:Naloxone; D009020:Morphine; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins