Cloning of a cDNA encoding an Acanthamoeba castellanii PDI-like protein.
We have cloned, from Acanthamoeba castellanii, the cDNA encoding a new member of the protein disulfide isomerase (PDI)-like protein family. The new PDI-like protein contains two highly conserved thioredeoxin-like domains, each about 100 amino acids in length. However, the A. castellanii PDI-like protein differs from other members in many aspects, including the overall organization and isoelectric point. Southern and Northern analyses demonstrate that the PDI-like protein is encoded by a single-copy gene which is transcribed to generate a 1500-nucleotide mRNA.
Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405.
J M
JM
Wong
E
E
Bateman
D000048:Acanthamoeba / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan; D007535:Isomerases / Q000235:genetics*; D008969:Molecular Sequence Data; D019704:Protein Disulfide-Isomerases; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D007535:Isomerases; D019704:Protein Disulfide-Isomerases