Assignment of the human heart tetrodotoxin-resistant voltage-gated Na+ channel alpha-subunit gene (SCN5A) to band 3p21.
The chromosomal location of SCN5A, the gene encoding the principal voltage-gated Na+ channel expressed in human heart, has been determined by three independent methodologies: somatic cell hybrid mapping, chromosomal microdissection-polymerase chain reaction, and fluorescence in situ hybridization. The SCN5A gene was assigned to the short arm of chromosome 3 (band 3p21) by all three approaches. These data are further evidence that striated muscle Na+ channel genes are dispersed in the genome.
George
A L
AL
Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN.
Varkony
T A
TA
Drabkin
H A
HA
Han
J
J
Knops
J F
JF
Finley
W H
WH
Brown
G B
GB
Ward
D C
DC
Haas
M
M
D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D017931:DNA Primers; D004351:Drug Resistance; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012377:Rodentia; D015222:Sodium Channels / Q000187:drug effects / Q000235:genetics*; D013779:Tetrodotoxin / Q000494:pharmacology*
D017931:DNA Primers; D015222:Sodium Channels; D013779:Tetrodotoxin