Enhancement of mouse egg activation by the kinase inhibitor, 6-dimethylaminopurine (6-DMAP).
Metaphase arrest in vertebrate eggs is maintained by the action of cytostatic factor (CSF) on maturation promoting factor (MPF). In amphibian eggs, the kinase inhibitor, 6-dimethylaminopurine (6-DMAP) inactivates both CSF and MPF, resulting in the release from metaphase and entry into interphase. In the mouse, 6-DMAP induces nuclear formation in maturing oocytes, but not in eggs at metaphase II. We found that 6-DMAP accelerated the transition to interphase in mouse eggs treated with the parthenogenetic agents A23187, cycloheximide, or phorbol 12-myristate 13-acetate (PMA). Newly ovulated eggs, refractory to full activation by A23187, entered interphase when treated with A23187 and 6-DMAP in combination. Occasional batches of eggs formed nuclei in response to 6-DMAP alone. These results are discussed with regard to the possible effects of 6-DMAP on MPF and CSF.
Department of Zoology, University of Toronto, Ontario, Canada.
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Moses
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Masui
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D007399:Interphase / Q000187:drug effects; D016200:Maturation-Promoting Factor / Q000187:drug effects; D000090204:Mesothelin; D008677:Metaphase / Q000187:drug effects; D051379:Mice; D010063:Ovum / Q000166:cytology / Q000187:drug effects*; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*; D017231:Proto-Oncogene Proteins c-mos / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
C000715528:Msln protein, mouse; D000001:Calcimycin; C001020:N(6),N(6)-dimethyladenine; D003513:Cycloheximide; D010770:Phosphotransferases; D017231:Proto-Oncogene Proteins c-mos; D016200:Maturation-Promoting Factor; D000090204:Mesothelin; D000225:Adenine; D013755:Tetradecanoylphorbol Acetate