[The 43KD polypeptide in the proteins of human lens].
The total proteins, water--soluble proteins and the urea--soluble proteins of the lens from fetal, adult and senile cataract were determined by SDS--PAGE. It was found that there was a 43KD polypeptide in the capsular--epithelium of all lens stated above. The band on SDS-PAGE corresponding to 43KD polypeptide was wide in the water--soluble proteins of the cortex and nucleus of the lenses obtained from over 14-year-old individuals. It became blurred in the urea--soluble proteins of cortex and nucleus of lens with aging, whereas it became almost disappeared in the senile cataract lens.
Zhongshan Ophthalmic Center, Sun Yat-Sen University of Medical Sciences, Guangzhou, China.
X
X
Cao
Y
Y
Chen
S
S
Liang
Q
Q
Huang
S
S
Li
W
W
Mao
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002386:Cataract / Q000378:metabolism*; D003459:Crystallins / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry* / Q000196:embryology; D010455:Peptides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D003459:Crystallins; D010455:Peptides