Differential effects of ethanol consumption on synthesis of cytoplasmic and mitochondrial encoded subunits of the ATP synthase.
The relative concentrations of several subunits of the mitochondrial F0.F1-ATP synthase were determined in mitochondria and submitochondrial particles prepared from the livers of ethanol-fed and control rats. The polypeptides were separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis and the stained gels were analyzed by densitometry for the relative concentrations of the ATP synthase subunits. A significant decrease in the relative concentration of the mitochondrial gene product, ATPase subunit 8, was observed in mitochondria and submitochondrial particles from ethanol-fed animals. The relative concentration of the other mitochondrial encoded ATPase subunit, ATPase 6, was also depressed, as confirmed in submitochondrial particles. In contrast, there were no significant ethanol-related depressions in subunits alpha, beta, and OSCP of the F0.F1 or the adenine nucleotide carrier in intact mitochondria. These results demonstrate that ethanol consumption causes a decrease in the content of mitochondrial synthesized subunits 6 and 8 whereas no effect is exerted on the concentrations of nuclear gene products of the ATP synthase complex. Likewise, the adenine nucleotide transporter, also a nuclear gene product, is unaffected by ethanol consumption.
Department of Biochemistry, Wake Forest University Medical Center, Winston-Salem, North Carolina 27157-1016.
W B
WB
Coleman
A
A
Cahill
P
P
Ivester
C C
CC
Cunningham
D000428:Alcohol Drinking / Q000009:adverse effects*; D000818:Animals; D003593:Cytoplasm / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects*; D006180:Proton-Translocating ATPases / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
D006180:Proton-Translocating ATPases