Isolation and characterization of cDNA clones specifically expressed in testicular germ cells.
We have cloned cDNAs involved in germ cell-specific expression. For this, a subtracted cDNA library was generated by subtracting cDNAs derived from supporting cells of mutant testis from wild-type testis cDNAs. Detailed analyses of mRNA expression revealed that the genes corresponding to the cloned cDNAs were exclusively expressed in testes and were developmentally controlled.
Tanaka
H
H
Department of Science for Laboratory Animal Experimentation, Osaka University, Japan.
Yoshimura
Y
Y
Nishina
Y
Y
Nozaki
M
M
Nojima
H
H
Nishimune
Y
Y
D051339:Actin Depolymerizing Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051347:CapZ Actin Capping Protein; D002454:Cell Differentiation; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D051345:Destrin; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D008297:Male; D051379:Mice; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D013087:Spermatids / Q000378:metabolism*; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000378:metabolism; D013735:Testicular Hormones / Q000737:chemistry / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic
D051339:Actin Depolymerizing Factors; C090518:CAPZA3 protein, mouse; D051347:CapZ Actin Capping Protein; D018076:DNA, Complementary; D051345:Destrin; D008840:Microfilament Proteins; D015347:RNA Probes; D013735:Testicular Hormones