Ubiquitin changes in human biceps muscle following exercise-induced damage.
Changes in ubiquitin levels were characterized in human biceps muscle following high-force eccentric exercise. Volunteers performed damaging eccentric-isokinetic actions of the biceps muscle with the non-dominant arm. Protein extracts of biopsy tissue samples taken two days post-exercise were run on SDS polyacrylamide gels, analyzed densitometrically and revealed a 64% higher level of a protein band at 12 kD. New monoclonal immunoblotting techniques identified the band as free ubiquitin. On these blots, free ubiquitin increased in the exercised sample by 55% over the control, and ubiquitin conjugates of varying molecular weights follow a similar pattern. The changes seen in both free and conjugated ubiquitin suggest that their increases are involved in the response to exercise-induced muscle damage.
Thompson
H S
HS
Department of Biological Sciences, Smith College, Northampton, MA 01063.
Scordilis
S P
SP
D001706:Biopsy; D004591:Electrophoresis, Polyacrylamide Gel; D015444:Exercise; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism / Q000473:pathology*; D005082:Physical Exertion; D014452:Ubiquitins / Q000302:isolation & purification / Q000378:metabolism*
D014452:Ubiquitins