Tissue-specific expression of type 1 angiotensin II receptor subtypes. An in situ hybridization study.
The angiotensin II type 1 (AT1) receptor in murine species exists as two isoforms (AT1A and AT1B) encoded by two different genes. Both subtypes have a 9/10 homology in the coding sequence of their mRNA. We examined organs of adult rats (liver, pituitary gland, adrenal gland, kidney, heart, and lung) to study the differential expression of these two genes in target tissues for angiotensin II. AT1A and AT1B mRNAs were detected by in situ hybridization using specific riboprobes for the 3' noncoding region of the mRNAs that have the lowest homology (approximately 6/10). Only AT1A was expressed in the liver, heart, and lung, and only AT1B was expressed in the anterior pituitary, where most cells were positive. In the adrenal gland, AT1A mRNA was detected in the zona glomerulosa and medulla and AT1B in the glomerulosa. In the kidney, AT1A mRNA was the predominant isoform (mesangial and juxtaglomerular cells, proximal tubules, vasa recta, and interstitial cells), but AT1B was also detected in mesangial and juxtaglomerular cells and in the renal pelvis. The results of this in situ detection suggest a tissue-selective regulation of AT1A and AT1B mRNAs. This tissue specificity may constitute a prerequisite condition if the two angiotensin II receptor subtypes, which are pharmacologically similar, are to selectively modulate the various effects of angiotensin II in the different target tissues.
INSERM U36, Collège de France, Laboratoire de Médecine Expérimentale, Paris.
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Shanmugam
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Sibony
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D000311:Adrenal Glands / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D005260:Female; D015870:Gene Expression; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism; D007672:Kidney Cortex / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D010902:Pituitary Gland / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011945:Receptors, Angiotensin / Q000096:biosynthesis* / Q000145:classification; D013462:Sulfur Radioisotopes
D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D013462:Sulfur Radioisotopes; D000804:Angiotensin II