Controlled comparison of interceed and amniotic membrane graft in the prevention of postoperative adhesions in the rabbit uterine horn model.
OBJECTIVE
To evaluate the effects of Interceed TC7 (Johnson & Johnson Medical, New Brunswick, NJ) and gamma-irradiated human amniotic membrane graft in prevention of postoperative adhesions in the rabbit uterine horn model.


METHODS
A conventional animal laboratory.


METHODS
Virgin, female New Zealand white rabbits.


METHODS
Both uterine horns were subjected to clean transverse cuts 1 cm apart at the antimesenteric border. The left uterine horn was left uncovered in each case to act as a control. The right uterine horn was randomly covered with either Interceed TC7 or human amniotic membrane.


METHODS
Adhesion score was evaluated 4 weeks after the initial surgery. Samples of each uterine horn were collected for histopathological studies, and subserosal inflammatory score.


RESULTS
Interceed resulted in higher adhesion scores and subserosal inflammation score than the control side (P = 0.03 and 0.05, respectively). A similar difference was found for amniotic membrane when compared with the uncovered control side (P = < 0.01 and 0.01, respectively).


CONCLUSIONS
Interceed TC7 failed to have the preventive effect on adhesion formation it is claimed to possess. Amniotic membrane performed poorly too. Therefore, gentle tissue handling, irrigation and good surgical skills remain the most important prerequisite for adhesion prevention.
Department of Obstetrics and Gynecology, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
M
M
Arora
K A
KA
Jaroudi
C J
CJ
Hamilton
F
F
Dayel
D000650:Amnion / Q000637:transplantation*; D000818:Animals; D002483:Cellulose, Oxidized / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control*; D011247:Pregnancy; D011817:Rabbits; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control*; D014599:Uterus / Q000601:surgery*
D002483:Cellulose, Oxidized; C060002:INTERCEED