Neurons dissociated from neocortex fire with 'burst' and 'regular' trains of spikes.
Neurons acutely dissociated from neocortex slices of 14-16-day-old rats were patch-clamped in physiological conditions. Different pyramidal cells, spontaneously or in response to current steps, generate regular spiking and intrinsically bursting behaviour during long periods of time. We show that typical firing properties recorded in somatosensory neocortex slices are preserved in dissociated pyramidal neurons originating from the slices themselves, thus, providing a way for the related characterization of biophysical properties of currents in identified subtypes of pyramidal neurons.
Istituto Neurologico C. Besta, Milano, Italy.
E
E
Guatteo
A
A
Bacci
S
S
Franceschetti
G
G
Avanzini
E
E
Wanke
D000200:Action Potentials; D000818:Animals; D002540:Cerebral Cortex / Q000502:physiology*; D004558:Electric Stimulation; D005071:Evoked Potentials; D066298:In Vitro Techniques; D009474:Neurons / Q000502:physiology*; D011712:Pyramidal Tracts / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors