Analysis of BCR/ABL transcripts in chronic myeloid patients by nested PCR using an immunoassay-like detection format.
We describe an 'immunoassay-like' detection format, based on the AMPLICIS technique for the routine detection of BCR/ABL transcripts in leukaemic patients. The AMPLICIS technique is characterized as a nested PCR coupled to solid phase isotopic or non-isotopic detection of PCR products. Comparison between this assay and a conventional PCR technique using Southern-blot analysis provided a good correlation between the two procedures. The assay, easier and faster than the conventional one, gives access to automation and therefore appears well suited for the routine screening of BCR/ABL chimaeric mRNAs.
Hopital Saint-Antoine, Service de Biochimie B, Paris, France.
V
V
Barbu
B
B
Alpha-Bazin
D
D
Pepin
L
L
Morand-Joubert
C
C
Chypre
G
G
Bereziat
D001483:Base Sequence; D015139:Blotting, Southern; D002678:Chimera; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D016044:Fusion Proteins, bcr-abl