Expression of mRNA for atrial natriuretic peptide receptor guanylate cyclase (ANPRA) in human retina.
1. Guanylate cyclase plays an important role in the visual cycle. Here we report the mRNA expression for the atrial natriuretic peptide receptor type A form of guanylate cyclase (ANPRA) in human retina. 2. Polymerase chain reaction using two sets of primers on the cDNAs reverse-transcribed from human retinal poly(A)+ RNA amplified two products under two different reaction conditions. The primers used in the reaction were designed from the reported sequence of human placental ANPRA cDNA. 3. Sequencing of the amplified products showed 100% sequence homology to the human placental ANPRA gene. Northern blot analysis indicated the presence of a 4.4-kb ANPRA mRNA in human retina, similar to that present in human brain.
Pardhasaradhi
K
K
Section on Drug-Tissue Interaction, National Heart, Lung and Blood Institute, NIH, Bethesda, Maryland 20892.
Kutty
R K
RK
Gentleman
S
S
Krishna
G
G
D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D017931:DNA Primers; D005260:Female; D006162:Guanylate Cyclase / Q000096:biosynthesis*; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis*; D017461:Receptors, Atrial Natriuretic Factor / Q000096:biosynthesis*; D012160:Retina / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D012333:RNA, Messenger; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor