Effect of supernatant of peripheral blood mononuclear cells from nephrotic patients on the sulfated compound synthesis by cultured glomerular epithelial cell.
To identify the role of circulating immune factor(s) in the development of proteinuria, the supernatant of peripheral blood mononuclear cells (PBMC) were obtained from 15 patients with steroid-responsive nephrotic syndrome, and its effect on the synthesis of sulfated compound by cultured rat glomerular epithelial cell (GEC) were studied by 35S, 3H-leucine dual-isotope labelling technique. The result showed that the supernatant of PBMC from 9 patients without steroid treatment increased 35S uptake in GEC (P < 0.01), while there was no significant difference in 35S uptake between the 6 patients treated with prednisone (1.5-2.0 mg/kg.d-1) and 6 normal controls.
Pediatric Department, First Teaching Hospital, Beijing Medical University.
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Wang
D000293:Adolescent; D000818:Animals; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D004847:Epithelial Cells; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000096:biosynthesis*; D006801:Humans; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D009404:Nephrotic Syndrome / Q000188:drug therapy / Q000276:immunology*; D011241:Prednisone / Q000627:therapeutic use; D011509:Proteoglycans / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D011241:Prednisone; D011509:Proteoglycans