A region-specific microdissection library for human chromosome 2p23-->p21 and the analysis of an interstitial deletion of 2p21.
A region-specific library of human chromosome 2p23-->p21 was constructed using microdissection and microcloning techniques. Analysis of 94 single-copy microclones from the library showed that 64% were derived from the dissected region. Ten microclones were further mapped to the 2p21 region using a patient with an interstitial deletion of 2p21 and displaying holoprosencephaly, an abnormal embryonic development in midbrain and midface.
Eleanor Roosevelt Institute for Cancer Research, Denver, CO.
F T
FT
Kao
J
J
Yu
J
J
Qi
S
S
Tong
M
M
Muenke
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular / Q000379:methods; D006224:Cricetinae; D015247:Deoxyribonuclease HindIII; D004210:Dissection / Q000379:methods; D005314:Embryonic and Fetal Development; D005145:Face / Q000002:abnormalities / Q000196:embryology; D015723:Gene Library; D016142:Holoprosencephaly / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D008636:Mesencephalon / Q000002:abnormalities / Q000196:embryology; D015183:Restriction Mapping
D015247:Deoxyribonuclease HindIII