Expression of adhesion molecules, platelet-activating factor, and chemokines by Kaposi's sarcoma cells.
The present study was designed to investigate whether cells cultured from Kaposi's sarcoma (KS), a vascular tumor with a prominent leukocyte infiltration, express molecules important for the recruitment and activation of leukocytes. KS cells expressed intercellular adhesion molecule-1, which was augmented by exposure to IL-1 beta or TNF-alpha. Unlike endothelial cells, resting or cytokine-activated KS cells did not express appreciable levels of intercellular adhesion molecule-2, vascular cell adhesion molecule-1, and E-selectin on their surface. Weak expression of vascular cell adhesion molecule-1 mRNA was detectable by Northern blot analysis and, most clearly, by PCR analysis. Upon exposure to inflammatory cytokines, KS cells produced the attractant/activating lipid platelet-activating factor. KS cells expressed appreciable levels of the chemotactic cytokines, monocyte chemotactic protein-1 (MCP-1) and IL-8, as determined by Northern blot analysis, immunoassay, or bioassay. Chemokine production was augmented by IL-1 beta or TNF-alpha. MCP-1 expression was also detected in KS lesions by in situ hybridization. The set of molecules identified in the present study is probably important in determining the prominent leukocyte infiltration observed in KS. Tumor-associated leukocytes may amplify autocrine/paracrine circuits that sustain KS proliferation and contribute to recruitment of host vascular cells.
Institute for Pharmacological Research Mario Negri, Milan, Italy.
F L
FL
Sciacca
M
M
Stürzl
F
F
Bussolino
M
M
Sironi
H
H
Brandstetter
C
C
Zietz
D
D
Zhou
C
C
Matteucci
G
G
Peri
S
S
Sozzani
D001483:Base Sequence; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002630:Chemotactic Factors / Q000096:biosynthesis*; D005434:Flow Cytometry; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010972:Platelet Activating Factor / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000276:immunology*; D014407:Tumor Cells, Cultured
D015815:Cell Adhesion Molecules; D002630:Chemotactic Factors; D010972:Platelet Activating Factor