Toward a physical map of human chromosome 10: isolation of 183 YACs representing 80 loci and regional assignment of 94 YACs by fluorescence in situ hybridization.
One hundred eighty-three YACs carrying human chromosome 10 sequences were isolated from multigenome equivalent libraries by PCR-based screening for the presence of 80 different chromosome 10-specific STSs. Ninety-four of the isolated YACs, representing 52 genes and DNA segments, were mapped to regions of chromosome 10 by fluorescence in situ hybridization. The results localized 26 DNA segments to cytogenetic bands for the first time. About 37% (35/94) of the YACs hybridized to more than one chromosomal location: 31 to other chromosomes in addition to chromosome 10 and 4 to 2 distinct locations on chromosome 10. These results are consistent with the number of chimeric YACs expected from these libraries but may also reflect the presence of 2 or more YACs within a single clone or the presence of low copy repeated elements within the genome. This STS anchor screening effort resulted in the identification of 69 contigs, with 7 contigs consisting of 2 anchors each and 1 contig consisting of 5 anchors. All linked STSs were multiply linked by at least 2 independent YACs. These anchored YACs span the entire chromosome and appear to cover 15% of chromosome 10.
Department of Human and Molecular Genetics, Collaborative Research, Inc., Waltham, Massachusetts 02154.
D T
DT
Moir
T E
TE
Dorman
J C
JC
Day
N S
NS
Ma
M T
MT
Wang
J I
JI
Mao
D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D002879:Chromosomes, Human, Pair 10; D015723:Gene Library; D008040:Genetic Linkage; D005819:Genetic Markers; D016045:Human Genome Project; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016324:Sequence Tagged Sites
D005819:Genetic Markers