Separation of 24 dansylamino acids by capillary electrophoresis with a non-ionic surfactant.
The separation of 24 dansylamino acids was investigated by capillary electrophoresis with an additive of micelles of a non-ionic surfactant, Tween 20. Although two pairs of peaks, norvaline and methionine derivatives, and didansyltyrosine and solvent (methanol), did not show good resolution, other dansylamino acids were well separated within 70 min using 100 mM Tween 20 and pH 2.40. The theoretical plate numbers calculated for dansylamino acids were 28,000-111,000 with a 19-cm capillary column.
Matsubara
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Faculty of Science, Himeji Institute of Technology, Hyogo, Japan.
Terabe
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S
D000596:Amino Acids / Q000302:isolation & purification*; D003619:Dansyl Compounds / Q000032:analysis* / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D008823:Micelles; D011136:Polysorbates
D000596:Amino Acids; D003619:Dansyl Compounds; D008823:Micelles; D011136:Polysorbates