In vitro effect of 5-fluorouracil, verapamil and hyperthermia on the human pancreatic adenocarcinoma cell line ASPC-1.
The purpose of this study was to assess the efficacy of verapamil (20 microM) and hyperthermia (42 degrees C) as modifiers of 5-fluorouracil (5-FU) used at different concentrations, in inhibiting the growth of human pancreatic adenocarcinoma cells. Combined verapamil and hyperthermia treatment significantly decreased cell count by 63.8% when compared to the control. Verapamil drastically enhanced the growth inhibitory effect of 5-FU at all concentrations. At high-concentration 5-FU (50 micrograms/ml), verapamil and hyperthermia had a synergistic growth-inhibitory effect and caused a significant decrease in cell count by 81.4% in comparison to the control. The modalities analyzed in this study require further investigation and have potential for clinical applicability to pancreatic cancer therapy in the future.
Shchepotin
I B
IB
Lombardi Cancer Center, Georgetown University, Washington, D.C.
Buras
R R
RR
Nauta
R J
RJ
Shabahang
M
M
Soldatenkov
V A
VA
Evans
S R
SR
D000230:Adenocarcinoma / Q000473:pathology* / Q000628:therapy; D002452:Cell Count / Q000187:drug effects; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D006979:Hyperthermia, Induced; D010190:Pancreatic Neoplasms / Q000473:pathology* / Q000628:therapy; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014700:Verapamil / Q000494:pharmacology*
D014700:Verapamil; D005472:Fluorouracil