Effect of Ni(II) on tissue hydrogen peroxide content in mice as inferred from glutathione and glutathione disulfide measurements.
Studies on Ni(II)-induced carcinogenesis have suggested that oxidative damage caused by Ni(II) may in part be due to increased tissue H2O2 formation. However, there is lack of evidence in vivo. Because of limitations of available methods for direct measurement of the in vivo rate of H2O2 formation in animals, Ni(II)-induced production of H2O2 was estimated from changes in the rate of glutathione disulfide (GSSG) formation. Male B6C3F1 mice (6-8 wk old) were injected i.p. with 170 mumol NiAc2/kg. Biliary efflux and liver, kidney, and lung levels of glutathione (GSH and GSSG) were determined 0-2 h after treatment. In spite of slight increases in tissue GSSG levels by Ni(II), there was no significant change in the biliary efflux of GSSG. Pretreatment with 50 mg/kg (i.p.) of bis-chloroethyl-nitrosourea (BCNU), an inhibitor of GSSG reductase, did not augment the effects of Ni(II) on GSSG formation significantly. Based on these observations, it was apparent that Ni(II) did not change the concentration of H2O2 significantly in vivo.
Chang
J
J
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
Jaeschke
H
H
Randerath
K
K
D000818:Animals; D001646:Bile / Q000737:chemistry / Q000187:drug effects; D002330:Carmustine / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D005978:Glutathione / Q000031:analogs & derivatives / Q000187:drug effects / Q000378:metabolism; D019803:Glutathione Disulfide; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D009532:Nickel / Q000494:pharmacology*
D009532:Nickel; D004247:DNA; D006861:Hydrogen Peroxide; D005978:Glutathione; D002330:Carmustine; D019803:Glutathione Disulfide