Regions US6 and US7 of herpes simplex virus type 1 DNA encoding glycoproteins D and I may influence neuroinvasivity.
Recombinants were prepared by replacing a 1931 bp region of the BamHI J fragment (0.906-0.920) of the pathogenic ANGpath DNA-coding for glycoprotein D (gD) and a part of glycoprotein I (gI)--by the corresponding sequence of nonpathogenic KOS DNA (Kaerner et al., 1991) and tested in DBA/2 mice. The strain ANGpath and the control recombinant ANGpath/gD-gIpath, prepared by back transfer of the given ANGpath DNA fragment into ANGpath/gD-gIdellacZ+ DNA, were pathogenic after intraperitoneal inoculation. In contrast, mice infected with the strain KOS and the low-pathogenic recombinant ANGpath/gD-gIKOS survived peripheral virus administration. Both the strain KOS and the low-pathogenic recombinant ANDpath/gD-gIKOS spread by bloodstream to spleen, liver and adrenal glands but did not multiply in spinal cord. Nevertheless, the antigen of low-pathogenic recombinant ANGpath/gD-gIKOS was found in retroperitoneal vegetative nerves and ganglia. On the other hand, the strain ANGpath and the pathogenic recombinant ANGpath/gD-gIpath multiplied in cerebrospinal nerves and spinal cord causing typical hind leg paralysis.
Institute of Virology, Slovak Academy of Sciences, Bratislava.
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Rajcáni
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Kostál
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Kaerner
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics*; D006561:Herpes Simplex / Q000209:etiology / Q000473:pathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D051379:Mice; D008811:Mice, Inbred DBA; D009422:Nervous System Diseases / Q000209:etiology / Q000473:pathology / Q000821:virology; D009928:Organ Specificity; D011995:Recombination, Genetic; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000235:genetics*; D014774:Virulence / Q000235:genetics
D000956:Antigens, Viral; D004279:DNA, Viral; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; C064959:glycoprotein I, herpes simplex virus type 1