Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum.
The complementary DNA (PsU BC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys85) found in UBCs so far described is also conserved in the P. sativum sequence.
Woo
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Department of Plant Pathology, University of Arizona, Tucson 85721.
Brigham
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Hawes
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D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D044763:Ubiquitin-Conjugating Enzymes
D018076:DNA, Complementary; D012333:RNA, Messenger; D044763:Ubiquitin-Conjugating Enzymes; C063109:ubiquitin-conjugating enzyme UBC4; D008025:Ligases