The promoter of a pine photosynthetic gene allows expression of a beta-glucuronidase reporter gene in transgenic rice plants in a light-independent but tissue-specific manner.
In angiosperms, the expression of the cab gene that encodes the chlorophyll a/b-binding protein of PSII is light-regulated. However, the pine cab gene is expressed in a light-independent but cell-type-specific manner. In the present study, the cab-6 promoter (1.7 kbp) from pine was fused to a beta-glucuronidase (GUS) reporter gene and the chimeric gene was introduced into rice protoplasts by electroporation. The GUS expression was studied in the resultant transgenic rice plants. Expression of GUS at a substantial level was confirmed in primary leaves of dark-germinated rice seedlings, and no obvious effect of light on the GUS activity was observed. The expression of GUS was restricted to photosynthetic tissues. The pine cab-6 promoter is, thus, sufficient for induction of light-independent but cell-type-specific expression in cells of a monocot, as is the case in the original pine cells.
Yamamoto
N
N
National Institute of Agrobiological Resources, Ibaraki, Japan.
Tada
Y
Y
Fujimura
T
T
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D008027:Light; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012275:Oryza / Q000235:genetics / Q000528:radiation effects; D010788:Photosynthesis / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014197:Trees / Q000235:genetics
D017931:DNA Primers; D005966:Glucuronidase