Second messenger changes of atrial natriuretic factor and brain natriuretic peptide in kidneys of cardiomyopathic hamsters.
The effects of natriuretic peptides in kidney are blunted in congestive heart failure (CHF). The aim of this study is to examine the changes of atrial natriuretic factor (ANF) and brain natriuretic peptide (BNP) second messenger productions in CHF. Experiments were conducted on 300-day-old normal and cardiomyopathic hamsters. Blood was collected for ANF measurement. cGMP accumulation studies were done in glomeruli upon ANF and BNP stimulation, and in inner medullary collecting duct (IMCD) cells upon ANF stimulation. Higher plasma ANF levels were found in cardiomyopathic hamsters (811.3 +/- 124.6 vs. 166.6 +/- 13 pg/ml, p < 0.01). ANF-stimulated cGMP accumulations in glomeruli and IMCD cells were higher in cardiomyopathic hamsters. Increased BNP-stimulated cGMP accumulations were also observed in cardiomyopathic hamster glomeruli. These results suggest that the renal hyporesponsiveness to natriuretic peptides in CHF in not due to attenuated ANF and BNP second messenger productions.
Department of Medicine, University of British Columbia, University Hospital, Vancouver, Canada.
J K
JK
Luk
E F
EF
Wong
N L
NL
Wong
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000502:physiology*; D002303:Cardiac Output, Low / Q000378:metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D006152:Cyclic GMP / Q000378:metabolism* / Q000493:pharmacokinetics; D006333:Heart Failure / Q000201:enzymology*; D007678:Kidney Glomerulus / Q000201:enzymology*; D007685:Kidney Tubules, Collecting / Q000201:enzymology / Q000473:pathology; D008297:Male; D008647:Mesocricetus; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000502:physiology*; D015290:Second Messenger Systems
D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP