Effect of polylactic acid on osteoinduction of demineralized bone: preliminary study of the usefulness of polylactic acid as a carrier of bone morphogenetic protein.
To evaluate the usefulness of polylactic acid (PLA), a bioabsorbable and plastic polymer, as a carrier of bone morphogenetic protein (BMP), a preliminary study has been carried out to investigate any negative effect of PLA on osteoinduction of demineralized bone (DB). PLA (10,600 mol. wt) was mixed with DB particles (1-1.5 mm square) prepared from rat femurs and laid subcutaneously on the intercostal muscle of 4-week-old Wistar rats. The PLA/DB pellets were harvested at 2, 4, 8 and 24 weeks after the operation, and prepared for light microscopic examination. Histological examination revealed cartilage formation at 2 weeks and new bone formation at 4 weeks. Extensive bone and marrow formation were observed at 24 weeks. PLA was gradually absorbed and completely disappeared at 24 weeks to be replaced by connective tissue. These results demonstrate that PLA does not have a negative influence on the osteoinductive activity and that PLA could well be a promising bioabsorbable carrier of BMP.
Saitoh
H
H
Department of Operative Dentistry, Hiroshima University School of Dentistry, Japan.
Takata
T
T
Nikai
H
H
Shintani
H
H
Hyon
S H
SH
Ikada
Y
Y
D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D018775:Bone Demineralization Technique; D001853:Bone Marrow / Q000254:growth & development; D001857:Bone Matrix / Q000254:growth & development; D019485:Bone Morphogenetic Proteins; D002356:Cartilage / Q000254:growth & development; D004337:Drug Carriers / Q000633:toxicity; D005549:Foreign-Body Reaction / Q000209:etiology; D006097:Granulation Tissue / Q000254:growth & development; D006133:Growth Substances / Q000008:administration & dosage*; D007773:Lactates / Q000633:toxicity*; D019344:Lactic Acid; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters; D011108:Polymers / Q000633:toxicity*; D011506:Proteins / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D006133:Growth Substances; D007773:Lactates; D011091:Polyesters; D011108:Polymers; D011506:Proteins; D019344:Lactic Acid; C033616:poly(lactide)