Binding of low molecular weight heparin (Tinzaparin sodium) to bovine endothelial cells in vitro.
Heparinase depolymerized low molecular weight (LMW) heparin (Tinzaparin sodium, Logiparin) was radiolabelled by catalytic tritiation to high specific radioactivity and the binding to fetal bovine heart endothelial (FBHE) cells was studied at 4 degrees C and 37 degrees C. The binding was found to be time dependent and saturable. Two classes of binding sites could be distinguished from Scatchard analysis at both temperatures: One with high affinity (KD = 0.027 microM at 4 degrees C, KD = 0.012 microM at 37 degrees C) and another with very low affinity (KD = 69 microM at 4 degrees C and 37 microM at 37 degrees C). The binding reversibility was affected by the temperature indicating internalization of a fraction of the bound LMW heparin. At 4 degrees C only 11% of the specifically bound heparin was bound irreversibly. At 37 degrees C the non displaceable fraction accounted for 28% of the specifically bound LMW heparin. This work demonstrates that tinzaparin sodium binds specifically to endothelial cells. This binding may be useful in interpreting pharmacokinetic properties of this low molecular weight heparin.
Novo Nordisk A/S, Gentofte, Denmark.
A
A
Larnkjaer
P B
PB
Ostergaard
H J
HJ
Flodgaard
D000818:Animals; D001665:Binding Sites / Q000502:physiology; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005343:Fibrinolytic Agents / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D006495:Heparin, Low-Molecular-Weight / Q000378:metabolism*; D000078222:Tinzaparin; D014316:Tritium
D005343:Fibrinolytic Agents; D006495:Heparin, Low-Molecular-Weight; D014316:Tritium; D000078222:Tinzaparin; D006493:Heparin