Growth control of influenza A virus by M1 protein: analysis of transfectant viruses carrying the chimeric M gene.
Analysis of fast-growing reassortants (AWM viruses) of influenza A virus produced by mixed infection with a fast-growing WSN strain and a slowly growing Aichi strain indicated that the M gene plays a role in the regulation of virus growth rate at an early step of infection (J. Yasuda, T. Toyoda, M. Nakayama, and A. Ishihama, Arch. Virol. 133:283-294, 1993). To determine which of the two M gene products, M1 or M2, is responsible for the growth rate control, one recombinant WSN virus (CWA) clone possessing a chimeric M gene (WSN M1-Aichi M2) was generated by using an improved reverse genetics and transfection system. The recombinant CWA virus retained the phenotype of both large plaque formation and early onset of virus growth. This indicates that the WSN M1 protein is responsible for rapid virus growth.
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
J
J
Yasuda
D J
DJ
Bucher
A
A
Ishihama
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D017931:DNA Primers; D005814:Genes, Viral; D009980:Influenza A virus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D007668:Kidney; D007700:Kinetics; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014162:Transfection; D014763:Viral Matrix Proteins / Q000096:biosynthesis / Q000378:metabolism*; D010948:Viral Plaque Assay
D017931:DNA Primers; D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins