The novel ion pump rhodopsins from Haloarcula form a family independent from both the bacteriorhodopsin and archaerhodopsin families/tribes.
Extreme halophiles newly collected from Argentine salt flats were characterized, in one of which, Haloarcula (sp. arg-1), light-driven retinal protein ion pumps were found. The proton pump, cruxrhodopsin-1, shows amino acid sequence homologies of 52% to bacteriorhodopsin and 48% to archaerhodopsin-1. The anion pump, cruxhalorhodopsin-1, identified partially as a 394bp polymerase chain reaction product, shows homologies of 70% to halorhodopsin, and 72% to pharaonis halorhodopsin. The ion pumps (and possibly sensors still to be found) in Haloarcula sp. arg-1, which constitute the cruxrhodopsin-1 family, are distinct from the bacteriorhodopsin and the archaerhodopsin families/tribes.
Department of Biology, School of Science, Nagoya University, Japan.
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M
Tateno
K
K
Ihara
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Y
Mukohata
D000595:Amino Acid Sequence; D019843:Archaeal Proteins; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000502:physiology; D001436:Bacteriorhodopsins / Q000145:classification / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D006216:Halobacteriaceae / Q000235:genetics*; D016623:Ion Pumps / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D008027:Light; D008566:Membranes / Q000378:metabolism; D008969:Molecular Sequence Data; D012168:Retinal Pigments / Q000145:classification / Q000235:genetics / Q000502:physiology; D012243:Rhodopsin / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017494:Proton Pumps
D019843:Archaeal Proteins; D001426:Bacterial Proteins; D001436:Bacteriorhodopsins; D016623:Ion Pumps; D012168:Retinal Pigments; D012243:Rhodopsin; C055476:archaerhodopsin protein, Archaea; D017494:Proton Pumps