Propolis extracts are effective against Trypanosoma cruzi and have an impact on its interaction with host cells.
Propolis, a natural resin produced by honey bees, that displays strong antimicrobial activity, has been used as a chemotherapeutic agent since ancient times. The anti-protozoan properties of different propolis extracts were studied regarding T. cruzi and its interaction with host cells. Ethanolic (EEP) and dimethylsulphoxide extracts (DEP) were both active against the three forms of the parasite, with the former being more active than the latter against the vertebrate forms, amastigotes and trypomastigotes. Total lysis of bloodstream trypomastigotes was observed after 24 h in the presence of EEP at a concentration of 100 micrograms/ml. The effect was found to be temperature dependent. Treatment of infected peritoneal macrophages and heart muscle cells with EEP strongly inhibited infection levels. The utilization of propolis as a possible antitrypanosomal agent is discussed.
Departamento de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
K O
KO
Higashi
S L
SL
de Castro
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide; D000431:Ethanol; D006790:Host-Parasite Interactions / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D011429:Propolis / Q000494:pharmacology*; D012997:Solvents; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development
D012997:Solvents; D014344:Trypanocidal Agents; D000431:Ethanol; D011429:Propolis; D004121:Dimethyl Sulfoxide