[Preliminary observation on the detection of schistosome circulating antigen and the immunocomplex by dot-ELISA].
Serum samples were collected from normal rabbits, normal persons, Schistosoma japonicum-infected rabbits and human cases respectively, and were treated by TCA precipitation and PEG precipitation, and subsequently detected by dot-ELISA. The results showed that both the supernatants of TCA-treated antisera from infected human cases and rabbits and the precipitants of PEG-treated antisera of the same origins showed positive reactions, while no positive reactions were found for sera from normal rabbits and normal persons. It was exhibited that dot-ELISA detected not only the circulating antigen of schistosomes but also the immunocomplex.
Department of Parasitology, Shanghai Second Medical University.
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D000818:Animals; D000936:Antigen-Antibody Complex / Q000097:blood*; D000947:Antigens, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000947:Antigens, Helminth