MRI contrast enhancement by Gd-DTPA-monoclonal antibody in 9L glioma rats.
To achieve a tissue-specific enhancement in diagnosis of brain tumor, a magnetic resonance imaging (MRI) study was performed using conjugate of Gd-DTPA and monoclonal antibody (MoAb) against 9L glioma cells. Fisher 344 strain rats were used for this study. MoAb against 9L glioma cells was conjugated with Gd-DTPA according to the method of Hnatowich et al. (1983) and used for the MRI study. The gadolinium (Gd) concentration in the Gd-MoAb injected to the rats was 0.01-0.03 mmol/kg. The enhancement effect increased gradually and persisted for 24 hours after the injection. This was longer than Gd-DTPA, which showed a peak of enhancement effect within 30 minutes after injection and was washed out within 120 min. This result was compatible with scintigraphy studies using 125I labeled anti 9L monoclonal antibody, in which the accumulation of the 125I antibody increased at 24, 48 and 72 hours after the injection. By using tumor-specific contrast agents such as Gd-MoAb, it may be possible to differentiate among tumor, perifocal edema and radiation injury.
Department of Neurosurgery, University of Tsukuba, Ibaraki, Japan.
A
A
Matsumura
Y
Y
Shibata
K
K
Nakagawa
T
T
Nose
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001921:Brain / Q000473:pathology; D001929:Brain Edema / Q000473:pathology*; D001932:Brain Neoplasms / Q000276:immunology / Q000473:pathology*; D003287:Contrast Media; D019786:Gadolinium DTPA; D005910:Glioma / Q000276:immunology / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009368:Neoplasm Transplantation; D009942:Organometallic Compounds / Q000493:pharmacokinetics; D004369:Pentetic Acid / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D051381:Rats; D011916:Rats, Inbred F344
D000911:Antibodies, Monoclonal; D003287:Contrast Media; D009942:Organometallic Compounds; D004369:Pentetic Acid; D019786:Gadolinium DTPA