A member of the reoviridae (DpRV) has a ploidy-specific genomic segment in the wasp Diadromus pulchellus (Hymenoptera).
Wild and laboratory populations of the parasitoid wasp Diadromus pulchellus are infected with a new member of the reoviridae (DpRV) described in this paper. The particles of this virus possess two capsid shells (diameters: 35 and 70 nm) made up of 11 proteins. The virus is present mainly in the gut of the wasp, with smaller quantities in its venom gland. The genome of virus particles purified from haploid insects (functional males) contains 10 segments, whereas virus from diploid insects (females and sterile diploid males) contains a supernumerary 3.33-kb segment. The sequence of this dsRNA segment revealed that it is a triplicated 1050-bp motif which is 97.5% similar to the 5' region of one of the 10 basic segments, the 3.80-kb segment.
Institut de Biocénotique Expérimentale des Agrosystèmes, Faculté des Sciences, Tours, France.
A
A
Rabouille
Y
Y
Bigot
J M
JM
Drezen
P Y
PY
Sizaret
M H
MH
Hamelin
G
G
Periquet
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D016679:Genome, Viral; D007700:Kinetics; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D011003:Ploidies; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism; D014863:Wasps / Q000254:growth & development / Q000821:virology*
D017931:DNA Primers; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins