Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging.
The tomato Cf-9 gene confers resistance to infection by races of the fungus Cladosporium fulvum that carry the avirulence gene Avr9. The Cf-9 gene was isolated by transposon tagging with the maize transposable element Dissociation. The DNA sequence of Cf-9 encodes a putative membrane-anchored extracytoplasmic glycoprotein. The predicted protein shows homology to the receptor domain of several receptor-like protein kinases in Arabidopsis, to antifungal polygalacturonase-inhibiting proteins in plants, and to other members of the leucine-rich repeat family of proteins. This structure is consistent with that of a receptor that could bind Avr9 peptide and activate plant defense.
Jones
D A
DA
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Thomas
C M
CM
Hammond-Kosack
K E
KE
Balint-Kurti
P J
PJ
Jones
J D
JD
D000595:Amino Acid Sequence; D002963:Cladosporium / Q000235:genetics / Q000502:physiology*; D016384:Consensus Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D005656:Fungal Proteins / Q000235:genetics; D018390:Gene Targeting; D017343:Genes, Plant; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006031:Glycosylation; D018551:Solanum lycopersicum / Q000737:chemistry / Q000235:genetics* / Q000382:microbiology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D017931:DNA Primers; D004251:DNA Transposable Elements; D005656:Fungal Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D010940:Plant Proteins; C073605:AVR9 protein, Cladosporium fulvum