Synthesis of diazoluminomelanin (DALM) in HL-60 cells for possible use as a cellular-level microwave dosimeter.
Chemical and bacterial synthesis of a thermochemiluminescent polymer known as diazoluminomelanin (DALM) has been previously reported. This paper focuses on the intracellular synthesis of aminomelanin (AM) in mammalian cell lines and subsequent DALM synthesis from this core molecule. B16 melanoma cells, HL-60 myeloid leukemia cells, and RAW 264.7 macrophages show AM and DALM production. Macroscopic image analysis of HL-60 cell lysates containing DALM using the Quantitative Luminescence Imaging System (QLIS) showed increased chemiluminescence (CL) with increased microwave power input and increased temperature. This work represents a first step toward the goal of microscopic radiofrequency dosimetry of individual DALM-loaded cells using image analysis.
Armstrong Laboratory (AL/OERT), Brooks Air Force Base, Texas 78235-5102.
J G
JG
Bruno
J L
JL
Kiel
D001704:Biopolymers; D002455:Cell Division / Q000528:radiation effects*; D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D008543:Melanins / Q000032:analysis* / Q000096:biosynthesis* / Q000378:metabolism / Q000528:radiation effects; D008546:Melanoma, Experimental; D008872:Microwaves; D008962:Models, Theoretical; D012044:Regression Analysis; D013696:Temperature; D014407:Tumor Cells, Cultured
D001704:Biopolymers; D008543:Melanins; C063567:diazoluminolmelanin; D008165:Luminol