Potential of machine-vision light microscopy in toxicologic pathology.
Major developments in machine-vision light microscopy and in reagent chemistry have led to a renaissance and revolution in the use of the light microscope in biology, biotechnology, and medicine. The potential use of this technology in the field of toxicologic pathology is discussed. It is suggested that a combination of investigating living cells and tissues and fixed samples using the new technologies will lead to understanding mechanisms of toxicity. Examples of the use of the methods in basic cell biology and medicine are presented.
Taylor
D L
DL
Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213.
DeBiasio
R
R
LaRocca
G
G
Pane
D
D
Post
P
P
Kolega
J
J
Giuliano
K
K
Burton
K
K
Gough
B
B
Dow
A
A
D000818:Animals; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D008853:Microscopy / Q000379:methods*; D014116:Toxicology / Q000379:methods*
D005404:Fixatives