Coated-bead assay for microbial cell-associated and extracellular protease activity.
An assay utilizing latex beads coated with substrates of 125I-labelled connective tissue proteins is described as an alternative method for quantitating cell-associated and extracellular proteolytic activity of micro-organisms. Proteolytic activity is a function of the amount of 125I-labelled peptides released into the incubation mixture.
Department of Marine Pathology, Center for Biological Research, La Paz, Mexico.
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Ascencio
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Wadström
D002176:Candida albicans / Q000201:enzymology*; D010450:Endopeptidases / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D007457:Iodine Radioisotopes; D008863:Microspheres; D013211:Staphylococcus aureus / Q000201:enzymology*
D016326:Extracellular Matrix Proteins; D007457:Iodine Radioisotopes; D010450:Endopeptidases