A frequently amplified region in Leishmania contains a gene conserved in prokaryotes and eukaryotes.
A 27.5-kb sequence that is present in an approx. 2-Mb chromosome in Leishmania also occurs as an inverted dimer in a multicopy, 55-kb circular molecule (LD1) in Leishmania infantum ITMAP263. Sequence analysis of a 7100-bp cloned segment from the circular molecule revealed three open reading frames (ORFs). The ORFs are likely to have protein coding function by a number of criteria, including Northern blot analyses. The amino acid (aa) sequences deduced from two ORFs showed no similarity to other sequences in the databases. The C-terminal aa sequence from the third ORF is related (22-29% identity, 57-71% similarity) to a family of genes conserved in bacteria and humans. One member (sfhB) of the gene family in Escherichia coli appears to have a role in regulation of cell growth.
Seattle Biomedical Research Institute, WA 98109-1651.
P J
PJ
Myler
G M
GM
Venkataraman
M J
MJ
Lodes
K D
KD
Stuart
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017124:Conserved Sequence; D016054:DNA, Protozoan; D005057:Eukaryotic Cells / Q000378:metabolism; D005784:Gene Amplification; D007891:Leishmania / Q000235:genetics*; D007893:Leishmania donovani / Q000235:genetics; D018314:Leishmania infantum / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011387:Prokaryotic Cells / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D016054:DNA, Protozoan; D015800:Protozoan Proteins