The analysis of cocaine and its metabolites by liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS).
The method for simultaneous determination of cocaine and its four metabolites (benzoylecgonine, ecgonine methyl ester, ecgonine and norcocaine) in urine by liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS) was studied. The mass spectra showed the quasi-molecular ions, [M+H]+ as the base peak. LC/APCI-MS analysis was performed by focusing the characteristic ions at m/ = 186, 290, 200, 304 and 290 for ecgonine, benzoylecgonine, ecgonine methyl ester, cocaine and norcocaine, respectively. Cocaine and its four metabolites were well separated by high performance liquid chromatography (HPLC). The recoveries of cocaine and its metabolites from the spiked urine were 40.3-94.7% by solid-phase extraction with two type cartridges (Bond Elut Certify and Bond Elut SCX).
Forensic Science Laboratory, Osaka Prefectural Police HQ 1-3-18 Hommachi, Japan.
M
M
Nishikawa
K
K
Nakajima
M
M
Tatsuno
F
F
Kasuya
K
K
Igarashi
M
M
Fukui
H
H
Tsuchihashi
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003042:Cocaine / Q000031:analogs & derivatives / Q000378:metabolism* / Q000652:urine*; D005069:Evaluation Studies as Topic; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018629:Spectrometry, Mass, Secondary Ion / Q000295:instrumentation / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods*
C008671:norcocaine; C005618:benzoylecgonine; C005617:ecgonine; D003042:Cocaine; C034524:ecgonine methyl ester