Effects of methylprednisolone on nucleus pulposus-induced nerve root injury.
METHODS
The effects of intervention by intravenous injection of methylprednisolone to reduce the nerve root injury after epidural application of autologous nucleus pulposus was studied in an experimental model on the pig cauda equina in 20 animals.


METHODS
Nucleus pulposus was harvested from a lumbar disc. After lowering the pH of the nucleus pulposus to 3.5 it was placed onto the sacrococcygeal cauda equina. Fifteen of the pigs received a single intravenous injection of 30 mg/kg methylprednisolone, 5 minutes, 24 hours, or 48 hours, respectively, after the application. After 7 days, the nerve conduction velocity was determined, and biopsies of the cauda equina was examined by lightmicroscopy.


RESULTS
In the five pigs that did not receive any methylprednisolone treatment, nerve conduction velocity was reduced, whereas it was normal in the pigs treated 5 minutes and 24 hours after nucleus pulposus application. In pigs treated after 48 hours, nerve conduction velocity was reduced only slightly. At the light microscopic level, significant changes occurred in all series.


CONCLUSIONS
This study indicates that the nucleus pulposus-induced effects on nerve function in an experimental pig model may be reduced dramatically by high-dose methylprednisolone administration within 24-48 hours after epidural application of autologous nucleus pulposus. The light microscopic changes were probably not significant for the nerve function. Instead, a morphologic explanation on a subcellular level should probably be sought.
Department of Orthopaedics, University of Gothenburg, Sahlgren Hospital, Sweden.
K
K
Olmarker
G
G
Byröd
M
M
Cornefjord
C
C
Nordborg
B
B
Rydevik
D000818:Animals; D002420:Cauda Equina / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D004576:Electromyography; D007403:Intervertebral Disc; D007405:Intervertebral Disc Displacement / Q000150:complications; D008159:Lumbar Vertebrae; D008775:Methylprednisolone / Q000008:administration & dosage / Q000494:pharmacology*; D009431:Neural Conduction / Q000187:drug effects / Q000502:physiology; D013552:Swine; D013997:Time Factors
D008775:Methylprednisolone