Presence of antitumor activities in the milk collected from gossypol-treated dairy cows.
Two human breast carcinoma cell lines (MCF-7, MCF-7 Adr) and a rat esophageal cancer cell line (RE-B2T) were used to evaluate the antiproliferative potential of gossypol (GP)-containing milk (GP-Milk), which was collected from Brown Swiss dairy cows treated daily with federally allowable 450 ppm of GP for 6 days. Treatment of the cultured cancer cells with GP-Milk for 24 h significantly inhibited the rates of 3H-thymidine incorporation during the ensuing 3-h period in all three tumorigenic cell lines. The inhibitory effects of GP-Milk occurred in a dose-dependent manner in all cases, but the calculated ED50 varied with cell lines. ED50 for GP-Milk was estimated at 10% for wild-type MCF-7 human breast cancer cells, 15% for multidrug-resistant MCF-7 Adr human breast cancer cells and 50% for RE-B2T rat esophageal carcinoma cells. The potential of GP-Milk as a dietary supplement for the prevention and/or treatment of human breast cancer is discussed in this paper.
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Ohio State University, Columbus 43210-1092.
Y F
YF
Hu
C J
CJ
Chang
R W
RW
Brueggemeier
Y C
YC
Lin
D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology / Q000517:prevention & control* / Q000628:therapy; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004938:Esophageal Neoplasms / Q000473:pathology / Q000517:prevention & control* / Q000628:therapy; D005260:Female; D006072:Gossypol / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D014407:Tumor Cells, Cultured
D008894:Milk Proteins; D006072:Gossypol