Expression of transforming growth factor-beta 1-4 in the chick limb bud mesenchymal cells: regulation by 1,25-dihydroxyvitamin D3.
When mesenchymal cells from the early chick limb bud (stage 23-24) are plated at high cell density they spontaneously undergo chondrogenesis, implicating extensive cell-to-cell interactions. In the past it has been shown that TGF-beta or 1,25 dihydroxyvitamin D3 influences this process. In the present study we investigated the expression of TGF-beta isoforms in the mesenchymal cells after treatment with 1,25-dihydroxyvitamin D3. We found that TGF-beta 2 was down-regulated due to the treatment. The size of the transcripts was the expected as described for sternum cartilage cells but with differential use preference.
Del Rio-Tsonis
K
K
Department of Biology, University of Dayton, OH 45469-2320.
Tsonis
P A
PA
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D015536:Down-Regulation; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014807:Vitamin D / Q000494:pharmacology
D016212:Transforming Growth Factor beta; D014807:Vitamin D; D002117:Calcitriol