Capillary zone electrophoretic determination of organic acids in cerebrospinal fluid from patients with central nervous system diseases.
Organic acids in cerebrospinal fluid (CSF) from patients with various central nervous system (CNS) diseases were determined by capillary zone electrophoresis (CZE). Under one of the two sets of conditions employed, several anionic components of CSF were separated into corresponding peaks on the electropherograms and determined. The other conditions employed were also useful in measurement of the lactate contents in CSF. The CSF levels of lactate and pyruvate and the ratios of lactate to pyruvate were elevated in patients with cerebral infarction and bacterial meningitis, whereas CSF ascorbate was reduced mainly in inflammatory disorders of the CNS. The results showed that CZE can become a powerful tool in the biochemical diagnosis of CNS diseases.
Department of Mental Health, Kyorin University School of Health Sciences, Tokyo, Japan.
A
A
Hiraoka
J
J
Akai
I
I
Tominaga
M
M
Hattori
H
H
Sasaki
T
T
Arato
D001927:Brain Diseases / Q000134:cerebrospinal fluid*; D002544:Cerebral Infarction / Q000134:cerebrospinal fluid; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D007773:Lactates / Q000134:cerebrospinal fluid*; D019344:Lactic Acid; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid; D011773:Pyruvates / Q000134:cerebrospinal fluid*; D019289:Pyruvic Acid; D013056:Spectrophotometry, Ultraviolet
D007773:Lactates; D011773:Pyruvates; D019344:Lactic Acid; D019289:Pyruvic Acid