Substitution of 2-aminoadenine and 5-methylcytosine for adenine and cytosine in hybridization probes increases the sensitivity of DNA fingerprinting.
An oligonucleotide (m5C-am2A-m5C)5 containing 2'-amino-deoxyadenosine (am2A) and 5-methyldeoxycytidine (m5C) residues has been synthesized and compared with unsubstituted pentadecadeoxyribonucleotide (CAC)5 as a hybridization probe for DNA fingerprinting. It was shown that considerably higher sensitivity can be achieved with the modified analog.
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
M I
MI
Prosnyak
S I
SI
Veselovskaya
V A
VA
Myasnikov
E J
EJ
Efremova
V K
VK
Potapov
S A
SA
Limborska
E D
ED
Sverdlov
D015075:2-Aminopurine / Q000031:analogs & derivatives*; D044503:5-Methylcytosine; D000225:Adenine; D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods; D003596:Cytosine / Q000031:analogs & derivatives*; D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015345:Oligonucleotide Probes; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; D000225:Adenine