Subcellular location and differential antibody specificity of arginase in tissue culture and whole animals.
Studies in man and other mammals have demonstrated the existence of two forms of arginase, a cytoplasmic form located primarily in liver and a mitochondrial form expressed in lesser amounts in a larger number of organs, but especially kidney. They appear to be encoded in different gene loci. Using a colloidal silica gradient separation technique, we have now located arginase in H4 cells, a rat hepatoma-derived line, to the cytoplasm and the arginase in human embryonic kidney-derived line, to the mitochondrion. Antibody prepared against A1 precipitates all the arginase from liver, 50% from kidney and none of the activity from human embryonic kidney (HEK) cells. An antibody prepared against partially purified All, by contrast, precipitates > 90% of arginase activity from HEK cells, half from kidney and virtually none from H4 cells or rat liver.
Department of Pediatrics, UCLA Center for the Health Sciences 90024-1759.
E B
EB
Spector
C P
CP
Jenkinson
M R
MR
Grigor
R M
RM
Kern
S D
SD
Cederbaum
D000818:Animals; D000918:Antibody Specificity; D001119:Arginase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D046508:Culture Techniques; D006801:Humans; D013347:Subcellular Fractions / Q000201:enzymology*
D001119:Arginase