X-ray crystallographic studies of eukaryotic transcription factors.
Our X-ray crystallographic work on eukaryotic transcription factors traverses the entire length of a typical class II nuclear gene promoter, including the TATA-box-binding protein, two b/HLH/Z promoter proximal binding factors (Max and USF), and an enhancer-binding factor (HNF-3 gamma). These high-resolution studies of specific protein/DNA interactions will be extended to include homologous proteins complexed with similar oligonucleotides and a systematic examination of the roles of individual amino acids and bases implicated in specific DNA binding. In the longer term, we will turn our attention to the myriad of protein/protein interactions occurring within TFIID and the PIC, and between the PIC and factors binding to promoter proximal and distal enhancer elements.
Burley
S K
SK
Laboratories of Molecular Biophysics, Rockefeller University, New York, New York 10021-6399.
Clark
K L
KL
Ferré-D'Amaré
A
A
Kim
J L
JL
Nikolov
D B
DB
D000818:Animals; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005057:Eukaryotic Cells; D051543:Hepatocyte Nuclear Factor 3-gamma; D006657:Histones / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D012319:RNA Polymerase II / Q000378:metabolism; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D006657:Histones; C067850:Myc associated factor X; D009687:Nuclear Proteins; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D051543:Hepatocyte Nuclear Factor 3-gamma; D004247:DNA; D012319:RNA Polymerase II