An improved method for butyrylcholinesterase phenotyping.
An improved method for the identification of butyrylcholinesterase phenotypes is proposed. It is based on modifications of a method that uses alpha-naphthyl acetate as substrate and DL-propranolol and Ro2-0683 as inhibitors. The proposed modifications make the method more rapid and increase the accuracy of the determinations of the phenotypes tested (BCHE U, BCHE UF, BCHE UA, BCHE AK, BCHE AF, and BCHE A). These modifications make the method even more adequate for population studies and clinical routine.
Department of Biochemistry, Federal University of Paraná, Curitiba, Brazil.
G
G
Picheth
C
C
Fadel-Picheth
S L
SL
Primo-Parmo
E A
EA
Chautard-Freire-Maia
M M
MM
Vieira
D000483:Alleles; D002091:Butyrylcholinesterase / Q000235:genetics*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D006801:Humans; D009283:Naphthol AS D Esterase / Q000494:pharmacology; D010641:Phenotype; D011433:Propranolol / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology
D002800:Cholinesterase Inhibitors; D000644:Quaternary Ammonium Compounds; C006266:Ro 20683; D011433:Propranolol; D009283:Naphthol AS D Esterase; D002091:Butyrylcholinesterase