Electrokinetic studies of inorganic coated capillaries.
The procedures for the preparation of silica capillaries coated with titanium oxide or aluminum oxide are developed. These inorganic coated capillaries are studied for their applicability in capillary electrophoresis. The points of zero charge are measured as pH 5 and pH 7 for titanium oxide- and aluminum oxide-coated capillaries, respectively. Both titanium oxide and aluminum oxide coatings give better protein separations in comparison to the use of fused-silica capillaries. Separation efficiency of lysozyme as model protein is measured in the range of 20,000 theoretical plates/m of inorganic coated capillaries. However, the hydrophobic interaction between proteins and modified capillary wall possibly contributes to the tailing of observed protein peaks.
Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County Campus 21228.
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Tsai
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Wu
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Lee
D000327:Adsorption; D000537:Aluminum Oxide / Q000737:chemistry; D004563:Electrochemistry; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000737:chemistry; D012822:Silicon Dioxide; D014025:Titanium / Q000737:chemistry
D011506:Proteins; C009495:titanium dioxide; D012822:Silicon Dioxide; D014025:Titanium; D000537:Aluminum Oxide