[Heterogenicity of hepatic L-pyruvate kinase in fasting animals].
Molecular forms of hepatic pyruvate kinase (PK) were separated by fractionating on DEAE-cellulose. 120-h food deprivation of rats entails a progressive decline in L-PK activity, but not the activity of M-type enzyme of the minor fraction. The rate of L-PK degradation depends on the fasting duration. A rapid inactivation phase is followed by a slower one with the speed constants 0.023 and 0.0065 h-1, respectively. To control the L-PK degradation rates in fasting diets, protein modification by phosphorylation can be employed.
Gorbach
Z V
ZV
Konovalenko
O V
OV
D000818:Animals; D005215:Fasting; D005508:Food Deprivation; D018740:Genetic Heterogeneity; D008099:Liver / Q000201:enzymology*; D008297:Male; D011770:Pyruvate Kinase / Q000235:genetics* / Q000378:metabolism; D051381:Rats
D011770:Pyruvate Kinase
Geterogennost l-pyruvatkinazy pecheni, vyiavliaemaia pri golodanii zhivotnykh.