Instrument to detect near-infrared fluorescence in solid-phase immunoassay.
The construction of a near-infrared (near-IR) fluorescence detector for measuring picomolar levels of near-IR laser dyes is described. The detector is designed for use in an immunoassay technique that employs antibodies labeled with near-IR polymethine cyanine dyes. These dyes possess spectral properties that are exclusive to the near-IR region (650-1100 nm). The instrumentation is characterized, including its hardware and data acquisition software components. The detector is capable of measuring fluorescence in both solution and solid-phase environments. Data on the detector's performance is presented.
Department of Chemistry, Georgia State University, Atlanta 30303.
R J
RJ
Williams
N
N
Narayanan
G A
GA
Casay
M
M
Lipowska
L
L
Strekowski
G
G
Patonay
J M
JM
Peralta
V C
VC
Tsang
D002232:Carbocyanines; D004396:Coloring Agents; D007118:Immunoassay; D007834:Lasers; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013055:Spectrophotometry, Infrared
D002232:Carbocyanines; D004396:Coloring Agents