Interferon-gamma levels in peritoneal dialysis effluents: relation to peritonitis.
As peritoneal macrophages require Interferon-gamma (IFN-gamma) for bacterial lysis, IFN-gamma levels were measured in peritoneal dialysis effluents. (PDE) by a specific radioimmunoassay. High IFN-gamma levels were found in patients with peritonitis compared to low levels in patients without peritonitis (mean 9.73 +/- 2.63 SE U/ml, N = 39 vs. 0.25 +/- 0.04, N = 32). IFN-gamma levels varied among different bacteria: Staph. aureus (highest: 23.4 +/- 5.7, N = 14), Staph. epidermidis (lower: 3.2 +/- 0.8, N = 13), other gram-positive (1.06 +/- 0.32, N = 6), gram-negative bacteria (lowest: 0.57 +/- 0.30, N = 6). After treatment of peritonitis levels decreased. In corresponding blood and PDE samples, by comparing IFN-gamma levels in 10 peritoneal dialysis patients (5 with peritonitis, 5 without), levels were raised only in PDE of patients with peritonitis, implying local IFN-gamma production. Total lymphocytes, T, B and monocyte subsets in patients' plasma and PDE did not differ, except for a higher number of mononuclear cells in PDE of patients with peritonitis (P < 0.05). Further investigation of in vitro IFN-gamma production in PDE with peritoneal monocytes, syngeneic host lymphocytes, and bacteria showed that Staph. aureus induced the highest levels of IFN-gamma and E. coli the lowest, in experiments with T cell enriched host lymphocytic fractions. We conclude that Staph. aureus peritonitis induces high levels of IFN-gamma in PDE, possibly by a T cell dependent superantigen response.
Department of Medicine, University of Alberta, Edmonton, Canada.
M K
MK
Dasgupta
M
M
Larabie
P F
PF
Halloran
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D015314:Dialysis Solutions / Q000032:analysis*; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis* / Q000096:biosynthesis; D007676:Kidney Failure, Chronic / Q000628:therapy; D007962:Leukocytes / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory; D010538:Peritonitis / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology; D011446:Prospective Studies; D011863:Radioimmunoassay; D013203:Staphylococcal Infections / Q000276:immunology
D015314:Dialysis Solutions; D007371:Interferon-gamma