Scavenging effects of tetramethylpyrazine on active oxygen free radicals.
The scavenging effects of tetramethylpyrazine (ligustrazine, Lig) isolated from Ligusticum wallichii Franch on active oxygen free radicals were studied using spin trapping technic and chemiluminescence methods. The scavenging rate of superoxide anion (O2-.) by Lig (25 mg.ml-1) was 100% in xanthine/xanthine oxidase (Xan/XO) system, and that of hydroxyl radical (OH-.) was 44% in Fenton's reaction. The scavenging rate of lipid peroxyl radical (LOO-.) by Lig (25 mg.ml-1) was 80% in peroxidizing microsome system. It is possible that Lig scavenged O2-. by catalyzing its spontaneous dismutation. The results showed that Lig had strong effects of scavenging cyotoxic oxygen free radicals (O2-., LOO-., OH-.).
First Affiliated Hospital of Hubei Medical University, Hubei Institute of Neuropsychiatry, Wuhan, China.
Z H
ZH
Zhang
S Z
SZ
Yu
Z T
ZT
Wang
B L
BL
Zhao
J W
JW
Hou
F J
FJ
Yang
W J
WJ
Xin
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D005609:Free Radicals; D008054:Lipid Peroxides / Q000378:metabolism; D008163:Luminescent Measurements; D008862:Microsomes, Liver / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats
D016166:Free Radical Scavengers; D005609:Free Radicals; D008054:Lipid Peroxides; D011719:Pyrazines; D010100:Oxygen; C017953:tetramethylpyrazine