Isolation of the novel agent from human stool samples that is associated with sporadic non-A, non-B hepatitis.
The agent(s) responsible for sporadic non-A, non-B hepatitis in humans was serially transmitted in rhesus monkeys by intravenous inoculation of the stool extract from a patient. A novel agent called HFV (hepatitis French [origin] virus) was present as 27- to 37-nm particles in the infectious stool extract. Hepatopathic lesions were noticed in infected monkeys during the acute phase of illness. The purified viral 27- to 37-nm particles consist of a double-stranded DNA of approximately 20 kb and are detected in infected monkey liver. Analysis of cell culture detects the approximately 20-kb-long viral DNA in stool samples from infected monkeys and sporadic enteric non-A, non-B hepatitis patients. Furthermore, the 27- to 37-nm viral particles were able to protect monkeys challenged with infectious stool extract. Our results indicate that 27- to 37-nm virus like particles are responsible for sporadic non-A, non-B hepatitis in rhesus monkeys.
Deka
N
N
National Institute of Immunology, New Delhi, India.
Sharma
M D
MD
Mukerjee
R
R
D000818:Animals; D000956:Antigens, Viral; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces; D016174:Hepacivirus / Q000302:isolation & purification* / Q000648:ultrastructure; D006526:Hepatitis C / Q000473:pathology / Q000635:transmission / Q000821:virology*; D006801:Humans; D008099:Liver / Q000473:pathology / Q000821:virology; D008253:Macaca mulatta; D008854:Microscopy, Electron; D009336:Necrosis; D013997:Time Factors; D017201:Virus Shedding
D000956:Antigens, Viral; D004279:DNA, Viral