Suppressive effects of IPD-1151T (suplatast-tosilate) on induction of mast cells from normal mouse splenocytes.
The influence of IPD-1151T (suplatast-tosilate) on murine mast cell induction was examined by in vitro cell culture. Splenocytes from BALB/c mice suspended in RPMI-1640 medium supplemented with interleukin-3 were cultured in the presence or absence of IPD-1151T. Half of the total volume of the medium was changed every 4-5 days. The number of mast cells increased with culture time and reached a peak on the 16th day when the cells were cultured without IPD-1151T. However, the growth of mast cells from the splenocyte culture was dose dependently inhibited by IPD-1151T. Furthermore, IPD-1151T inhibited the proliferation of mast cell progenitor cells, IC-2, but not that of splenocytes or mature mast cells.
First Department of Internal Medicine, School of Medicine, Showa University, Tokyo, Japan.
S
S
Konno
M
M
Adachi
K
K
Asano
Y
Y
Gonogami
K
K
Ikeda
K
K
Okamoto
T
T
Takahashi
D000818:Animals; D001190:Arylsulfonates / Q000493:pharmacokinetics / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004205:Cromolyn Sodium / Q000494:pharmacology; D006633:Histamine Antagonists / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013452:Sulfonium Compounds / Q000493:pharmacokinetics / Q000494:pharmacology*
D001190:Arylsulfonates; D006633:Histamine Antagonists; D013452:Sulfonium Compounds; C073028:suplatast tosilate; D004205:Cromolyn Sodium