The calpain-calpastatin system is regulated differently during human neuroblastoma cell differentiation to Schwannian and neuronal cells.
Changes in expression of calpains and calpastatin during differentiation in GOTO cells were examined using antibodies specific to calpains and calpastatin. Neuronal differentiation induced by dibutyryl cyclic AMP elicited a remarkable decrease in m-calpain. No marked changes in the levels of calpains were found in bromodeoxyuridine-induced Schwannian differentiation. Calpastatin was detected as a single band of 110k in undifferentiated and in neuronally differentiated cells by Western blot analysis. However, the appearance of a 120k species was detected in Schwannian differentiation associated with morphological change. The data show that marked changes in m-calpain and calpastatin occur in a differentiation-specific manner.
Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan.
Y
Y
Saito
T C
TC
Saido
K
K
Sano
S
S
Kawashima
D001973:Bromodeoxyuridine / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002154:Calpain / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism*; D009474:Neurons / Q000166:cytology*; D012583:Schwann Cells / Q000166:cytology*; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; D003994:Bucladesine; C033668:calpastatin; D002154:Calpain; D001973:Bromodeoxyuridine