Inositol lipid phosphorylation and breakdown in rat liver nuclei is affected by hydrocortisone blood levels.
The possibility that inositol lipid metabolism is related to nuclear events accompanying steroid hormone action has been investigated by comparing lipid phosphorylation and breakdown in normal rat liver nuclei and in hypo- and hypercortisolemic conditions. Lipid phosphorylation in vitro showed the presence of diacylglycerol (DAG)-, phosphatidylinositol (PI)- and phosphatidylinositol-4-phosphate (PIP)-kinase activity, with differences between total tissue homogenates and isolated nuclei, relevant to the treatment in vivo. Administration of hydrocortisone (HC) produced a marked decrease in the phosphorylated nuclear products without influencing the homogenate kinase activity. Under conditions which were optimal for the kinase activities, nuclear PIP-kinase was strongly increased in presence of a high blood level of HC whereas PI-kinase activity was reduced. From these observations it appears that the observed differences were due to specific modulation of kinase activities rather than to changes in the availability of substrates. The phosphoinositide-specific phospholipase C (PLC) activity was also investigated. In the presence of a high HC blood level, the phosphodiesteratic cleavage of PIP strongly increased, while that of phosphatidylinositol bisphosphate (PIP2) was similar in normal and hypercortisolemic conditions. Nuclear phosphoinositide hydrolysis was affected by PLC, beta and gamma isoforms, which were equally represented in all the conditions investigated, indicating that the observed changes of activity were due to a modulation rather than to a change in the amount of enzyme. These results suggest that inositol lipid metabolism plays a role in the nuclear modifications accompanying steroid hormone induction of transcriptional activity.
Santi
P
P
Istituto di Anatomia Umana Normale, Università di Bologna, Italy.
Marmiroli
S
S
Falcieri
E
E
Bertagnolo
V
V
Capitani
S
S
D000311:Adrenal Glands / Q000502:physiology; D000315:Adrenalectomy; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D006854:Hydrocortisone / Q000494:pharmacology*; D007294:Inositol / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley
D010716:Phosphatidylinositols; D007294:Inositol; D006854:Hydrocortisone