Specific detection of a verotoxin 2 variant, VT2vp1, by a bead-enzyme-linked immunosorbent assay.
A bead-enzyme-linked immunosorbent assay to specifically detect a Verotoxin 2 variant, VT2vp1, was developed. The sensitivity of the bead-ELISA was 200 pg/ml of the purified VT2vp1 and it did not react with 20 ng/ml of the purified VT2. The specificity of the bead-ELISA was examined with 107 strains of Verocytotoxin-producing Escherichia coli that include VT1-, VT2-, VT2vha-, VT2vhb- and VT2vp1-producing E. coli, and only VT2vp1-producing E. coli that were confirmed by VT2vp1-specific polymerase chain reaction gave positive results. It was noted that all 58 VT2vp1-producing E. coli strains were from pigs, but not from cows and humans.
Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.
C
C
Cao
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S
Yamasaki
Z
Z
Lin
H
H
Kurazono
Y
Y
Takeda
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004768:Enterotoxins / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008863:Microspheres; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D022641:Shiga Toxin 2; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D004768:Enterotoxins; D022641:Shiga Toxin 2