Chondroitin sulfate proteoglycan elevates cytoplasmic calcium in DRG neurons.
Proteoglycans have been implicated in neuronal path-finding during development, yet related second messenger and signaling systems are unknown. We have used the calcium indicator fura-2/AM to monitor cytoplasmic calcium ion concentration ([Ca2+]i) in chick dorsal root ganglion (DRG) neuronal growth cones elongating on laminin during contact with chondroitin sulfate proteoglycan (CSPG): (1) to determine whether there is a change in [Ca2+]i in neurons that contact CSPG, and (2) to determine whether changes in [Ca2+]i are necessary for inhibition of growth cone migration. The majority of DRG neurons responded to CSPG contact with a transient rise in [Ca2+]i (mean delta[Ca2+]i above resting level was 554 +/- 109 nM; P < 0.0001). The effect of CSPG contact was concentration dependent and required the carbohydrate moiety of CSPG. Addition of soluble CSPG did not elevate [Ca2+]i. Treatment with reagents that blocked plasma membrane calcium channels, or that perturbed intracellular Ca2+ stores, indicated that extracellular Ca2+ was the major source of the [Ca2+]i elevation, and that Ca2+ entry occurred through non-voltage-gated calcium channels. Although general Ca2+ channel blockers abolished the CSPG-induced [Ca2+]i rise, they did not abolish growth cone avoidance of surface-bound CSPG in these assays. We conclude: (1) that DRG neurons elevate [Ca2+]i in response to CSPG contact to levels that can modify cytoskeletal mechanisms of growth cone migration, and (2) that avoidance of substratum-bound CSPG may not be dependent upon elevated [Ca2+]i.
Snow
D M
DM
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55455.
Atkinson
P B
PB
Hassinger
T D
TD
Letourneau
P C
PC
Kater
S B
SB
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D002642:Chick Embryo; D011508:Chondroitin Sulfate Proteoglycans / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D007700:Kinetics; D007797:Laminin; D008858:Microscopy, Phase-Contrast; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D011508:Chondroitin Sulfate Proteoglycans; D007797:Laminin; D002118:Calcium