Function and regulation of the Arabidopsis floral homeotic gene PISTILLATA.
Mutations in the PISTILLATA (PI) gene of Arabidopsis thaliana cause homeotic conversion of petals to sepals and of stamens to carpels. It is thus classed as a B function floral homeotic gene and acts together with the product of the other known B function gene, APETALA3 (AP3). We have cloned PI and determined the time and places of its expression in developing flowers. Surprisingly, the initial patterns of PI and AP3 expression are different. By positive regulatory interactions between PI and AP3, later expression patterns are coincident or nearly coincident. The pattern of PI expression also depends on the activity of the floral development genes APETALA2 and SUPERMAN and on the activity of PI itself. The PI and APETALA3 proteins specifically associate in solution and so may act together in regulating PI and other genes.
Goto
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Division of Biology, California Institute of Technology, Pasadena 91125.
Meyerowitz
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D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005091:Exons; D015870:Gene Expression; D005801:Genes, Homeobox; D017343:Genes, Plant; D018398:Homeodomain Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D026161:MADS Domain Proteins; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
D029681:Arabidopsis Proteins; D017931:DNA Primers; D018398:Homeodomain Proteins; D026161:MADS Domain Proteins; C090225:PISTILLATA protein, Arabidopsis; D010940:Plant Proteins; D014157:Transcription Factors