A method for activity staining of peroxidase and beta-1,3-glucanase isozymes in polyacrylamide electrophoresis gels.
A procedure was developed for activity staining of peroxidase and beta-1,3-glucanase isozymes (a double staining procedure) in the same polyacrylamide gels using 3-amino-9-ethylcarbazol for peroxidase staining and laminarine and 2,3,5-triphenyltetrazolium chloride for glucanase staining. The procedure was used to detect peroxidase and beta-1,3-glucanase in different corn lines using plant leaf extract.
Shimoni
M
M
Division of Plant Pathology, Newe Ya'ar Research Center, Haifa, Israel.
D004591:Electrophoresis, Polyacrylamide Gel; D043326:Glucan 1,3-beta-Glucosidase; D007527:Isoenzymes / Q000032:analysis*; D009195:Peroxidase / Q000032:analysis*; D018515:Plant Leaves / Q000201:enzymology; D003313:Zea mays; D001617:beta-Glucosidase / Q000032:analysis*
D007527:Isoenzymes; D009195:Peroxidase; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase