[Detection of a 14;18 breakpoint cluster region in B-cell lymphomas by PCR methods].
Cytogenetic studies of B-cell lymphomas have shown a t(14;18) chromosomal translocation in the majority of cases. However, its detection is not always simple to achieve and a new approach is urgently required. Recently, molecular biological analysis has revealed rearrangements of IgH and/or bcl-2 genes in the DNA of lymphomas. In these cases, a translocation-specific fragment, MBR (bcl-2/JH), could be amplified by PCR. Our present study clearly demonstrated the presence of MBR PCR products in 7 out of 8 cases with B-cell lymphoma. Thus, the results indicate that amplification of MBR by PCR is useful for detection of a translocation and therefore clinical diagnosis of lymphoma cells.
Department of Clinical Laboratory and Medicine, School of Medicine, Tohoku University, Sendai.
T
T
Funato
A
A
Ohkawa
M
M
Chiba
T
T
Kano
A
A
Hoshino
T
T
Kawamura
T
T
Sasaki
R
R
Ichinohasama
T
T
Sawai
D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D015321:Gene Rearrangement; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic