Metabolic control of gluconeogenesis in transgenic mice: regulation of the phosphoenolpyruvate carboxykinase gene.
Diet and the effect of hormones were studied in transgenic mice bearing a part of the promoter of the gene for the gluconeogenic enzyme phosphoenolpyruvate carboxykinase. The domains of the gene promoter involved in tissue specificity, development, and response to diet were identified by analysis of expression of the mutated gene. Important differences were uncovered between results from earlier cell culture experiments and the results from more physiologic responses of the transgenic animals.
Wolf
G
G
Department of Nutritional Sciences, University of California, Berkeley.
D000818:Animals; D000242:Cyclic AMP / Q000502:physiology; D004032:Diet; D005786:Gene Expression Regulation; D005943:Gluconeogenesis; D006128:Growth; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics*
D000242:Cyclic AMP; D010729:Phosphoenolpyruvate Carboxykinase (GTP)