A physical map of the hyperthermophilic bacterium Aquifex pyrophilus chromosome.
A genomic map of the hyperthermophilic hydrogen-oxidizing bacterium Aquifex pyrophilus was established with NotI (GC/GGCCGC), SpeI (A/CTAGT), and XbaI (T/CTAGA). Linking clones and cross-hybridization of restriction fragments revealed a single circular chromosome of 1.6 Mbp. A single flagellin gene and six rRNA gene units were located on this map by Southern hybridization.
Shao
Z
Z
Lehrstuhl für Genetik, Universität Regensburg, Germany.
Mages
W
W
Schmitt
R
R
D005075:Biological Evolution; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016680:Genome, Bacterial; D006088:Gram-Negative Aerobic Bacteria / Q000235:genetics*; D006358:Hot Temperature; D008386:Marine Biology; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D014871:Water Microbiology
D004269:DNA, Bacterial; C056436:endodeoxyribonuclease SpeI; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases