Immunoassay for quantifying type I collagen degradation products in urine evaluated.
An enzyme-linked immunosorbent assay (ELISA) for measuring type I collagen degradation products in urine < 3 h was evaluated. The measuring range was 0.5-10.5 mg/L with a detection limit of 0.2 mg/L. Within-run and total CVs were 5.3% and 6.6%, respectively. Analytical recovery averaged 100%. The mean (+/- SD) concentrations in urine samples from a healthy premenopausal population (n = 102) were 250 +/- 110 mg/mol creatinine (Cr). A group of healthy postmenopausal women (n = 410) gave a mean value of 416 +/- 189 mg/mol Cr. Values obtained in the ELISA correlated well (r = 0.83) to HPLC values for the established bone resorption marker deoxypyridinoline (n = 214), slightly better than the correlation to hydroxyproline measurements (r = 0.78, n = 421). We conclude that the assay described here presents a useful tool for further elucidating the importance of type I collagen degradation products in urine.
Center for Clinical and Basic Research, Ballerup, Denmark.
M
M
Bonde
P
P
Qvist
C
C
Fledelius
B J
BJ
Riis
C
C
Christiansen
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000737:chemistry / Q000652:urine*; D003404:Creatinine / Q000652:urine; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005260:Female; D005615:Freezing; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000652:urine*; D017698:Postmenopause / Q000652:urine; D017697:Premenopause / Q000652:urine; D012016:Reference Values
D010446:Peptide Fragments; D003094:Collagen; D003404:Creatinine