[Determination of norfloxacin, an active dissociation product of silver norfloxacin, in guinea pig plasma and tissue by high performance liquid chromatography with column-switching].
A column switching HPLC method was established for the determination of norfloxacin, and active dissociation product of silver norfloxacin, in guinea pig plasma and tissues. The pre-treating column (50 mm x 5 mm ID) and analytical column (150 mm x 5 mm ID) were packed with mu-Bondapak C18, 37-50 microns and YWG-C18, 10 microns, respectively. A mixture of CH3OH-0.008 mol/L phosphate buffer-0.05 mol/L tetrabutylammoniun bromide (25:75:4) was used as the analytical mobile phase with a flow rate of 1 ml/min. The pre-treating mobile phase was 0.008 mol/L phosphate buffer with a flow rate of 3 ml/min. UV detection was made at 280 nm. The results were linear within a range of 2-128 micrograms/ml norfloxacin for plasma. The lowest detection limit was 0.25 microgram/ml for plasma. The mean recovery of the method was 102% with a relative standard deviation of less than 7%.
Department of Drug Analysis, School of Pharmacy, Changhai Hospital, Second Military Medical University.
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Cheng
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Gao
D000287:Administration, Topical; D000818:Animals; D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local / Q000032:analysis* / Q000627:therapeutic use; D002056:Burns / Q000188:drug therapy / Q000378:metabolism; D024841:Fluoroquinolones; D006168:Guinea Pigs; D008297:Male; D009643:Norfloxacin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000627:therapeutic use; D012867:Skin / Q000737:chemistry
D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local; D024841:Fluoroquinolones; C045339:silver norfloxacin; D009643:Norfloxacin