Expression of GATA-binding transcription factors in rat hepatocytes.
Recently, we demonstrated that a DNA-binding protein(s) is involved in transcriptional repression of the rat serine dehydratase gene in fetal liver. Here, we report that a GAT(A/T) motif is the target sequence for the DNA-binding protein. By screening a fetal liver cDNA library, we isolated a rat homolog of GATA-1. Rat GATA-1 expressed as a GST-fusion protein in E. coli bound to the GAT(A/T) motif in the serine dehydratase gene. Northern analysis show that GATA-1 and GATA-4 mRNAs are expressed in fetal hepatocytes.
Institute for Enzyme Research, University of Tokushima, Japan.
K
K
Matsuda
Y
Y
Kobune
C
C
Noda
A
A
Ichihara
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D050991:GATA4 Transcription Factor; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D012695:L-Serine Dehydratase / Q000235:genetics; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017354:Point Mutation / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D050991:GATA4 Transcription Factor; C494690:Gata1 protein, rat; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D012695:L-Serine Dehydratase