Sequence of the sheep interleukin-10-encoding cDNA.
The ovine interleukin-10 (oIL-10)-encoding cDNA has been cloned and sequenced using gene amplification by the polymerase chain reaction (PCR). We present the complete coding sequence of the ovine IL-10 gene, as well as the predicted amino acid (aa) sequence. The oIL10 DNA coding sequence is 531 nucleotides long and the mature protein product is predicted to be 18,367 Da, consisting of 158 aa, excluding a 19-aa N-terminal hydrophobic signal peptide. The oIL-10 protein is > 77% identical to pig and human IL-10, > 71% identical to rodent IL-10 and > 68% identical to viral IL-10.
Department of Veterinary Pathology, University of Edinburgh, UK.
B M
BM
Dutia
P
P
Hunt
D R
DR
Sargan
R G
RG
Dalziel
J
J
Hopkins
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry; D015186:Hominidae / Q000235:genetics; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000235:genetics*; D013552:Swine / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D016753:Interleukin-10