Impairment in mitochondrial cytochrome oxidase gene expression in Alzheimer disease.
Brains from 5 patients with Alzheimer's disease (AD) showed a 50%-65% decrease in mRNA levels of the mitochondrial-encoded cytochrome oxidase (COX, a marker of oxidative metabolism) subunits I and III in the middle temporal association neocortex, but not in the primary motor cortex, as compared to 5 control brains. The amount of mitochondrial-encoded 12S rRNA was not altered, nor was the amount of nuclear-encoded lactate dehydrogenase B mRNA (a marker of glycolytic metabolism). These data suggest that the decrease in COX I and III subunits mRNA in affected brain regions may contribute to reduced brain oxidative metabolism in AD.
Laboratory of Neurosciences, National Institute on Aging, Bethesda, MD 20892.
K
K
Chandrasekaran
T
T
Giordano
D R
DR
Brady
J
J
Stoll
L J
LJ
Martin
S I
SI
Rapoport
D000199:Actins / Q000096:biosynthesis; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology* / Q000473:pathology; D001344:Autopsy; D015415:Biomarkers; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology* / Q000473:pathology; D003576:Electron Transport Complex IV / Q000096:biosynthesis*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D046911:Macromolecular Substances; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012016:Reference Values
D000199:Actins; D015415:Biomarkers; D046911:Macromolecular Substances; D012333:RNA, Messenger; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D007770:L-Lactate Dehydrogenase; D003576:Electron Transport Complex IV