The phage lambda orf gene encodes a trans-acting factor that suppresses Escherichia coli recO, recR, and recF mutations for recombination of lambda but not of E. coli.
Bacteriophage lambda can recombine in recBC sbcB sbcC mutant cells by using its own gene orf, the Escherichia coli recO, recR, and recF genes, or both. Expression of an orf-containing plasmid complements the recombination defects of orf mutant phage. However, this clone does not complement a recO mutation for conjugational recombination or recO, recR, or recF mutations for repair of UV-induced DNA damage. A plasmid clone of orf produces a protein with an apparent molecular mass of 15 kDa.
Sawitzke
J A
JA
Institute of Molecular Biology, University of Oregon, Eugene 97403-1229.
Stahl
F W
FW
D001426:Bacterial Proteins / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D003227:Conjugation, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D029968:Escherichia coli Proteins; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D013489:Suppression, Genetic; D015534:Trans-Activators / Q000235:genetics*; D014466:Ultraviolet Rays / Q000009:adverse effects; D014764:Viral Proteins / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C081006:RecO protein, E coli; C489318:RecR protein, E coli; D015534:Trans-Activators; D014764:Viral Proteins; C489340:recF protein, E coli; C065831:RecR protein, Bacteria; C066218:recF protein, Bacteria