Loss of Apc+ in intestinal adenomas from Min mice.
Allelic loss at the Apc locus in spontaneously occurring intestinal adenomas from mice heterozygous for the ApcMin nonsense mutation was analyzed using a site-specific quantitative polymerase chain reaction assay. All 97 of the intestinal adenomas analyzed showed extensive loss of the wild-type Apc (Apc+) allele. Quantitative polymerase chain reaction analysis of loci linked to Apc indicated loss of the chromosome carrying Apc+. Only one copy of the homologue carrying ApcMin remained in the intestinal adenomas. Possible reasons for the difference in the mechanism of Apc+ loss between human and Min mouse intestinal adenomas are discussed.
McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison 53706.
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Luongo
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Moser
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Gledhill
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Dove
D000236:Adenoma / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003110:Colonic Neoplasms / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D017491:Genes, APC / Q000235:genetics*; D007414:Intestinal Neoplasms / Q000235:genetics*; D007421:Intestine, Small; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D015342:DNA Probes