Isolation and characterization of cDNA clones specifically expressed in testicular germ cells.
We have cloned cDNAs involved in germ cell-specific expression. For this, a subtracted cDNA library was generated by subtracting cDNAs derived from supporting cells of mutant testis from wild-type testis cDNAs. Detailed analyses of mRNA expression revealed that the genes corresponding to the cloned cDNAs were exclusively expressed in testes and were developmentally controlled.
Department of Science for Laboratory Animal Experimentation, Osaka University, Japan.
H
H
Tanaka
Y
Y
Yoshimura
Y
Y
Nishina
M
M
Nozaki
H
H
Nojima
Y
Y
Nishimune
D051339:Actin Depolymerizing Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051347:CapZ Actin Capping Protein; D002454:Cell Differentiation; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D051345:Destrin; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D008297:Male; D051379:Mice; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D013087:Spermatids / Q000378:metabolism*; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000378:metabolism; D013735:Testicular Hormones / Q000737:chemistry / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic
D051339:Actin Depolymerizing Factors; C090518:CAPZA3 protein, mouse; D051347:CapZ Actin Capping Protein; D018076:DNA, Complementary; D051345:Destrin; D008840:Microfilament Proteins; D015347:RNA Probes; D013735:Testicular Hormones