Oxidants act as chemorepellents in Paramecium by stimulating an electrogenic plasma membrane reductase activity.
Paramecium is a valuable eukaryotic model system for studying chemosensory transduction, adaptation and cellular sensory integration. While millimolar amounts of many attractants hyperpolarize and cause faster forward swimming, oxidants are repellents that depolarize and cause backward swimming at micromolar concentrations. The non-permeant oxidants cytochrome c, nitro blue tetrazolium and ferricyanide are repellents with half maximal concentrations of 0.4 microM, 2.2 microM and 100 microM respectively. In vivo reductase activities follow the same order of potencies. The concentration dependence of the cytochrome c reductase activity is well correlated with cytochrome c-induced depolarizations. This suggests that plasma membrane reduction of external cytochrome c is electrogenic, causing membrane depolarization and chemorepulsion. The reductase activity also appears to be voltage dependent. Depolarization by either K+, Na+, Ca++ or Mg++ correlates with inhibition of both in vivo reductase activities and cytochrome c-induced membrane potential changes. These responses were also seen in deciliated cells, showing that the body plasma membrane is sufficient for the response. Both chloroquine and diphenyleneiodonium inhibited reductase activities but only at unusually high concentrations. This activity showed no pH dependence in the physiological range. We propose that a plasma membrane bound NA-DPH-dependent reductase controls oxidant-induced depolarizations and consequent chemorepulsion.
Department of Biological Sciences State University of New York at Buffalo 14260.
T M
TM
Hennessey
L E
LE
Frego
J T
JT
Francis
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D016764:Cell Polarity; D002630:Chemotactic Factors / Q000494:pharmacology*; D002738:Chloroquine / Q000494:pharmacology; D003574:Cytochrome c Group / Q000494:pharmacology; D004594:Electrophysiology; D009861:Onium Compounds / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016805:Paramecium tetraurelia / Q000187:drug effects* / Q000201:enzymology* / Q000502:physiology
D002630:Chemotactic Factors; D003574:Cytochrome c Group; D009861:Onium Compounds; D016877:Oxidants; C007517:diphenyleneiodonium; D002738:Chloroquine; D010088:Oxidoreductases