Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled.
In the Drosophila embryo dishevelled (dsh) function is required by target cells in order to respond to wingless (wg, the homolog of Wnt-1), demonstrating a role for dsh in Wnt signal transduction. We have isolated a mouse homolog of the Drosophila dsh segment polarity gene. The 695-amino-acid protein encoded by the mouse dishevelled gene (Dvl-1) shares 50% identity (65% similarity) with dsh. Similarity searches of protein and DNA data bases revealed that Dvl-1 encodes an otherwise novel polypeptide. While no functional motifs were identified, one region of Dvl-1 was found to be similar to a domain of discs large-1 (dlg), a Drosophila tumor suppressor gene. In the embryo, Dvl-1 is expressed in most tissues, with uniformly high levels in the central nervous system. From 7.5 days postcoitum Dvl-1 is expressed throughout the developing brain and spinal cord, including those regions expressing Wnt-1 and En. Expression of Dvl-1 in adult mice was found to be widespread, with brain and testis exhibiting the highest levels. The majority of Dvl-1 expression in the adult cerebellum is in the granular cell layer, similar to the pattern seen for engrailed-2 (En-2). Throughout postnatal development of the brain Dvl-1 is highly expressed in areas of high neuronal cell density.
Sussman
D J
DJ
Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore 21201-1559.
Klingensmith
J
J
Salinas
P
P
Adams
P S
PS
Nusse
R
R
Perrimon
N
N
D048868:Adaptor Proteins, Signal Transducing; D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D017931:DNA Primers; D000072261:Dishevelled Proteins; D004330:Drosophila / Q000201:enzymology / Q000235:genetics*; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000502:physiology; D005314:Embryonic and Fetal Development; D015870:Gene Expression; D005865:Gestational Age; D006801:Humans; D008297:Male; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010750:Phosphoproteins; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013116:Spinal Cord / Q000196:embryology / Q000378:metabolism; D013737:Testis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D017931:DNA Primers; C000606578:DVL1 protein, human; D000072261:Dishevelled Proteins; D029721:Drosophila Proteins; C000606579:Dvl1 protein, mouse; D010750:Phosphoproteins; D011506:Proteins; D012333:RNA, Messenger; C000606588:dsh protein, Drosophila; D004247:DNA