Mannan-binding protein levels in human amniotic fluid during gestation and its interaction with collectin receptor from amnion cells.
Collectins are a group of soluble proteins, each of which has collagenous and non-collagenous globular domains, and may be complement associated and/or have lectin activity. The complement protein C1q is structurally and functionally related to the collectins. Members of the collectin family are mannan-binding protein (MBP), lung surfactant protein A, CL-43, conglutinin and lung surfactant protein D. All except the last of these proteins have been shown to interact with a single, widely distributed receptor, named collectin receptor (also called C1q receptor). It has been proposed that collectins play an important role as opsonins in innate immunity. MBP is a serum protein and has been shown to activate the classical complement pathway. MBP has also been shown to opsonize bacteria and enhance clearance of bacteria by phagocytosis. In this article we demonstrate the presence of MBP in amniotic fluid and the localization of collectin receptor on the surface of amniotic epithelium. MBP levels in amniotic fluid were found to increase sharply from about 32 weeks of gestation. Collectin receptor was purified from a cell line, FL, derived from amniotic epithelium and was shown to interact with MBP. These results suggest that MBP may play a role in the antibody-independent recognition and clearance of pathogens in the amniotic cavity, towards term.
Department of Biochemistry, Oxford University, U.K.
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Malhotra
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Willis
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Lopez Bernal
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Thiel
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Sim
D000595:Amino Acid Sequence; D000650:Amnion / Q000166:cytology / Q000378:metabolism*; D000653:Amniotic Fluid / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D037222:Collectins; D005455:Fluorescent Antibody Technique; D005865:Gestational Age; D006801:Humans; D018960:Hyaluronan Receptors; D008351:Mannans / Q000378:metabolism*; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism; D011951:Receptors, Complement / Q000302:isolation & purification / Q000378:metabolism*
C087299:C1QBP protein, human; D002352:Carrier Proteins; D037222:Collectins; D018960:Hyaluronan Receptors; D008351:Mannans; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D011951:Receptors, Complement; C032402:complement 1q receptor