Differential cytotoxic effects of gamma-linolenic acid on MG-63 and HeLa cells.
Gamma-linolenic acid (GLA) inhibited cell proliferation in MG-63 and HeLa cells. Different morphological lesions were present in dividing cells; abnormal spindle formation in MG-63 cells and chromosome hypercondensation in HeLa cells. Different types of cell death were also present in interphase cells, no apoptosis but only 6% pycnosis in MG-63 cells and 90% apoptosis in HeLa cells. In MG-63 cells immunofluorescence showed segregation of nucleoli components, abnormal spindle formation and decreased labelling of microtubuli during interphase. In HeLa interphase cells prominently labelled abnormally arranged microtubuli were observed. The effects of GLA on protein synthesis in synchronized cells were determined with [35S]-methionine incorporation and SDS-PAGE. Decreased protein synthesis in both G1- and S-phase MG-63 cells was present. In S-phase HeLa cells, proteins of approximately 40, 92 and 150 kD were markedly expressed. Signalling mechanisms involved in cell proliferation and cell death may be differently affected in MG-63 and HeLa cells.
Department of Physiology, University of Pretoria, South Africa.
M
M
de Kock
M L
ML
Lottering
J C
JC
Seegers
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects*; D002466:Cell Nucleolus / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000473:pathology*; D006801:Humans; D008870:Microtubules / Q000187:drug effects; D012516:Osteosarcoma / Q000473:pathology*; D014176:Protein Biosynthesis; D008941:Spindle Apparatus / Q000187:drug effects; D014407:Tumor Cells, Cultured; D017965:gamma-Linolenic Acid / Q000494:pharmacology*
D000970:Antineoplastic Agents; D017965:gamma-Linolenic Acid