Re-evaluation of conditions required for measurement of true alanine:glyoxylate aminotransferase activity in human liver: implications for the diagnosis of hyperoxaluria type I.
In this paper we studied the glyoxylate-dependent transamination of L-alanine and L-glutamate in human liver homogenates in order to develop a reliable method for the determination of true alanine:glyoxylate aminotransferase activity in liver homogenates from patients suspected to suffer from hyperoxaluria type I. Measurements were made according to two protocols described in literature in control human liver homogenates which were either untreated or treated with an antiserum raised against purified alanine:glyoxylate aminotransferase. The results obtained show that enzyme activity can best be determined at pH 8.0 as compared to pH 7.4 since the former leads to a higher sensitivity of the method. Alanine:glyoxylate aminotransferase activities measured at pH 8.0 are approximately 50% higher compared to the enzyme activities measured at pH 7.4. Accordingly, it is proposed to measure alanine:glyoxylate aminotransferase activity at pH 8.0 using the newly determined correction factor as described in this paper.
Department of Pediatrics and Clinical Biochemistry, University Hospital Amsterdam, The Netherlands.
V A
VA
Horváth
R J
RJ
Wanders
D000409:Alanine / Q000378:metabolism; D000410:Alanine Transaminase / Q000378:metabolism*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006960:Hyperoxaluria, Primary / Q000175:diagnosis* / Q000201:enzymology; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D011233:Precipitin Tests; D013379:Substrate Specificity; D014021:Tissue Preservation; D000637:Transaminases / Q000378:metabolism
D007106:Immune Sera; D018698:Glutamic Acid; D000637:Transaminases; C020649:glutamate-glyoxylate aminotransferase; D000410:Alanine Transaminase; C019635:Alanine-glyoxylate transaminase; D000409:Alanine