Modulation of ornithine decarboxylase activity by prolactin in seminal vesicles of the rat.
The stimulatory effect of prolactin on sexual accessory glands is well established, though the mechanism of trophic action remains poorly understood. We have therefore assessed the effect of high levels of prolactin on ornithine decarboxylase activity and polyamine content in seminal vesicles (SV) of adult rats. Hyperprolactinaemia was induced by implantation of tissue fragments of a prolactin-secreting tumour 7315a, and the rats killed at 35 days post-inoculation. Serum levels of prolactin were increased significantly (p < 0.001) in tumour-bearing rats. Levels of testosterone in serum were reduced markedly, whereas LH levels remained unchanged. SV weight in the experimental group was not significantly different from that in the corresponding control group. A clear increase in ornithine decarboxylase activity of SV was observed (p < 0.001) in the hyperprolactinaemic rats. However, there was no change in the polyamine content of SV in tumour-bearing rats, compared to the control group. These results indicate that ornithine decarboxylase activity in SV of adult rats is regulated mainly by prolactin. This experimental model may be useful for identification of some of the events involved in the trophic action of prolactin.
Instituto de Biología y Medicina Experimental, Buenos Aires, República Argentina.
S I
SI
Gonzalez
M O
MO
Suescun
S B
SB
Rulli
F
F
Estivariz
R S
RS
Calandra
D000818:Animals; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009929:Organ Size; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010911:Pituitary Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D011073:Polyamines / Q000378:metabolism*; D011388:Prolactin / Q000097:blood / Q000378:metabolism / Q000502:physiology*; D015175:Prolactinoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D011915:Rats, Inbred BUF; D012016:Reference Values; D012669:Seminal Vesicles / Q000201:enzymology* / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism
D011073:Polyamines; D013096:Spermine; D011388:Prolactin; D009955:Ornithine Decarboxylase; D013095:Spermidine; D011700:Putrescine