Long-lasting protection of sheep against bluetongue challenge after vaccination with virus-like particles: evidence for homologous and partial heterologous protection.
Insect cells co-infected with appropriate recombinant baculoviruses synthesize double-shelled, virus-like particles (VLPs) with bluetongue virus (BTV) VP2 proteins representing serotype 1 (BTV-1), 2 (BTV-2), 10 (BTV-10), 13 (BTV-13) or 17 (BTV-17) as previously reported for BTV-10 (French, T.J., Marshall, J.J.A. and Roy, P. J. Virol. 1990, 64, 5696-5700). The derived particles were purified and used to vaccine sheep, either as single VLP types (BTV-10, BTV-17) or as a combination of all five serotypes. Control sheep received saline. The virus-neutralizing antibody responses were measured. Depending on the experiment, the sheep were challenged with homologous (BTV-10, -13, -17) or selected heterologous (BTV-4, -11, -16) viruses either after 4 months or 14 months, and the disease, viraemias and clinical reactions monitored. The results indicated that two doses of 10 micrograms of VLPs elicited a long-lasting immune response which protected the sheep against challenge with the homologous virulent virus. In certain cases, partial protection was afforded against challenge by heterologous BTV serotypes.
Roy
P
P
Laboratory of Molecular Biophysics, University of Oxford, UK.
Bishop
D H
DH
LeBlois
H
H
Erasmus
B J
BJ
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000918:Antibody Specificity / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics / Q000276:immunology; D001819:Bluetongue / Q000276:immunology / Q000517:prevention & control*; D001820:Bluetongue virus / Q000235:genetics / Q000276:immunology*; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D015967:Gene Expression Regulation, Viral / Q000235:genetics / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D008854:Microscopy, Electron; D010957:Plasmids; D011994:Recombinant Proteins; D012756:Sheep; D014162:Transfection; D014611:Vaccination / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology; D014771:Virion / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure; D014774:Virulence / Q000276:immunology
D000914:Antibodies, Viral; D004279:DNA, Viral; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines