Na,K-ATPase subunit isoform expression in the guinea pig endolymphatic sac.
Na,K-ATPase subunit isoform expression was studied by immunocytochemistry in the guinea pig endolymphatic sac, using subunit isoform-specific polyclonal antibodies. Epithelial cells of the guinea pig endolymphatic sac were observed to contain the alpha 1- and beta 2-subunit isoforms, and to a lesser extent the beta 1-subunit isoform, of Na,K-ATPase. The alpha 1- and beta 2-subunit isoforms of Na,K-ATPase have been observed previously in other ion and fluid transporting regions of the membranous labyrinth, e.g., stria vascularis and vestibular dark cells. Combined data indicate that the alpha 1-, beta 2-form of Na,K-ATPase plays a role in the microhomeostasis of endolymph. The alpha 1 beta 2 Na,K-ATPase subunit isoform combination is different from typical ion and fluid transporting tissues, e.g., kidney and colon, and may reflect distinctive characteristics of inner ear Na,K-ATPase.
Department of Anatomy and Cell Biology, College of Medicine, University of Florida, Gainesville.
W J
WJ
ten Cate
L M
LM
Curtis
K E
KE
Rarey
D000818:Animals; D004710:Endolymph / Q000378:metabolism; D004712:Endolymphatic Sac / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D006168:Guinea Pigs; D006706:Homeostasis; D007150:Immunohistochemistry; D017136:Ion Transport; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D012444:Saccule and Utricle / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D013316:Stria Vascularis / Q000201:enzymology
D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase