The inhibitory effects of indomethacin, nordihydroguaiaretic acid, and pyrrolidinedithiocarbamate on ovulation and prostaglandin synthesis in yellow perch (Perca flavescens) follicle incubates.
This study specifically determined the correlation between ovulation and ovarian prostaglandin F (PGF) and prostaglandin E (PGE) levels in yellow perch follicles treated with several eicosanoid synthesis inhibitors. In incubates treated with indomethacin (IM; .00001 to 10.0 micrograms/ml) there was a significant dose-dependent correlation between ovulation and PGF/PGE levels, and between the two prostaglandins (PGs), further implicating PGs in the control of yellow perch ovulation. In incubates treated with nordihydroguaiaretic acid (NDGA), a traditional lipoxygenase inhibitor, there was a significant correlation between ovulation and PGF levels, yet there was no correlation between PGE levels and ovulation. A free-radical scavenger, pyrrolidinedithiocarbamate (PDTC) inhibited ovulation in a dose-dependent manner yet there was no correlation between either PGF or PGE levels and this inhibition, indicating that PDTC may block ovulation at other steps that involve free-radicals.
Bradley
J A
JA
Department of Biological Sciences, University of Notre Dame, IN 46556.
Goetz
F W
FW
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D009637:Masoprocol / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000502:physiology; D010060:Ovulation / Q000187:drug effects*; D010471:Perches / Q000502:physiology*; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D011759:Pyrrolidines / Q000494:pharmacology*; D013859:Thiocarbamates / Q000494:pharmacology*
D016166:Free Radical Scavengers; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D011759:Pyrrolidines; D013859:Thiocarbamates; C020972:pyrrolidine dithiocarbamic acid; D009637:Masoprocol; D007213:Indomethacin