Effect of trichosanthin and momorcharins on isolated rat hepatocytes.
alpha-Momorcharin, beta-momorcharin and trichosanthin increased the secretion of glutamate-pyruvate transaminase, lactate dehydrogenase and isocitrate dehydrogenase by isolated rat hepatocytes into the culture medium. This hepatotoxic effect of the proteins appeared to be concentration-dependent. The plant proteins induced morphological changes in hepatocytes including increased formation of cytoplasmic blebs and a reduction of microvilli on plasma membrane.
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T.
T B
TB
Ng
W K
WK
Liu
S W
SW
Tsao
H W
HW
Yeung
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D005688:Galactosamine / Q000494:pharmacology; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000187:drug effects; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012269:Ribosomal Proteins; D054788:Ribosome Inactivating Proteins; D015978:Trichosanthin / Q000302:isolation & purification / Q000494:pharmacology*
D010940:Plant Proteins; D012269:Ribosomal Proteins; D015978:Trichosanthin; D005688:Galactosamine; C039582:MMC protein, Momordica charantia; D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D000410:Alanine Transaminase; D054788:Ribosome Inactivating Proteins