Isolation of a cDNA clone encoding a novel membrane protein expressed in lymphocytes.
By subtractive hybridization using single-stranded phagemids with directional inserts, we isolated a mouse cDNA clone, LSM-1, from temperature-sensitive Abelson virus-transformed immature B cells whose differentiation was being induced after the shift from the permissive (35 degrees C) to the non-permissive temperature (39 degrees C). LSM-1, which encodes an as yet unknown peptide of 197 amino acids, has a putative signal sequence and a trans-membrane region, and is expressed in B- and T-cell lines, in spleen, thymus, and bone marrow of adult mice, and in embryos.
Department of Medicine III, Osaka University Medical School, Japan.
Y
Y
Shimizu
H
H
Ogawa
Y
Y
Oka
R
R
Mizuno
S
S
Sakoda
T
T
Kishimoto
H
H
Sugiyama
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010750:Phosphoproteins; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; C513303:Ptprcap protein, mouse