Synthesis and lymphatic transport of intestinal apolipoprotein A-IV in response to graded doses of triglyceride.
Factors regulating the intestinal synthesis and secretion of apoA-IV are incompletely understood. Although it is known that apoA-IV is stimulated by dietary lipid, it is not known whether graded doses of triglyceride elicit graded responses in apoA-IV synthesis and secretion. We used the chronic intestinal lymph-fistula rat to examine the effect of graded levels of intestinal triglyceride transport on secretion of apoA-IV into lymph and synthesis of apoA-IV in various regions of intestine. Rats were implanted with chronic duodenal and intestinal lymph duct cannulas and infused with lipid emulsions containing 5, 10, 20, 40, 80 mumol triolein (including [3H]triolein, 1.2 microCi), 8.7 mumol phosphatidylcholine, and 57 mumol sodium taurocholate in 3 ml phosphate-buffered saline (pH 6.4) at ml/h for 8 h. Lymph samples were collected for 1 h prior to and at 2, 4, 5, 6, 7, and 8 h after the start of lipid infusion. Lymphatic output of triglyceride, phospholipid, and apoA-IV was measured. Steady-state (8 h) content of radioactive lipid was also measured in the lumen and the wall of the gut. In separate studies rats were given infusions of either 5% glucose in saline ("fasting") or triolein emulsion (10, 20, 40, 80 mumol/h) for 8 h after which incorporation of [3H]leucine into apoA-IV in isolated, in situ loops of duodenum, proximal jejunum, mid-distal jejunum, and terminal ileum was measured. Lipid output increased dose-dependently in response to triolein infusion, with steady-state lipid transport achieved by 5 h after start of lipid infusion. Total recovery of 3H-labeled lipid was similar for all triolein doses. Increase in lymphatic apoA-IV output lagged that of triglyceride by 3-4 h, but by 8 h showed graded increases with triolein dose. ApoA-IV synthesis (apoA-IV radioactivity immunoprecipitated by an apoA-IV monospecific antibody and expressed as % of trichloroacetic acid-precipitable [3H]leucine radioactivity) in the duodenum and proximal jejunum showed a 2-fold increase (compared with fasting) in response to 10 mumol triolein/h, but no further increase at higher doses. However, apoA-IV synthesis in mid to distal jejunum increased dose-dependently.(ABSTRACT TRUNCATED AT 400 WORDS)
Department of Physiology, Louisiana State University School of Medicine, Shreveport 71130.
T J
TJ
Kalogeris
K
K
Fukagawa
P
P
Tso
D000818:Animals; D001054:Apolipoproteins A / Q000096:biosynthesis / Q000378:metabolism*; D001692:Biological Transport; D004305:Dose-Response Relationship, Drug; D007412:Intestinal Fistula / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D050356:Lipid Metabolism; D008208:Lymphatic System / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000008:administration & dosage*
D001054:Apolipoproteins A; D014280:Triglycerides; C022205:apolipoprotein A-IV