[Construction of a cDNA library of the erythrocytic stage of Plasmodium vivax].
Plasmodium vivax-infected blood samples were collected from patients in the field during malaria transmission season. Total RNA of the parasites was extracted by guanidine HCl/cesium chloride centrifugation. mRNA was purified through oligo-dT cellulose. Double stranded cDNA were synthesized with AMV reverse transcriptase by Huynh's method. lambda gt11 phage was used as the vector. A cDNA library of the erythrocytic stage P. vivax was constructed after recombination of DNA and package in vitro.
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine.
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D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D004912:Erythrocytes / Q000469:parasitology*; D015723:Gene Library; D010966:Plasmodium vivax / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger; D016053:RNA, Protozoan