Genetic organization and complete sequence of the Proteus mirabilis pmf fimbrial operon.
Proteus mirabilis, commonly associated with urinary tract infection, pyelonephritis and bacteremia, produces a number of fimbriae, including PMF (P. mirabilis fimbriae). Genes encoding PMF were isolated and the complete nucleotide (nt) sequence was determined. The pmf gene cluster, encoded by 5655 bp, predicts five polypeptides: PmfA (18,921 Da), PmfC (93,107 Da), PmfD (28,208 Da), PmfE (38,875 Da) and PmfF (19,661 Da). PmfA, PmfC, PmfD and PmfF share > 25% amino acid (aa) sequence identity with gene products of the pap, mrp and sfa fimbrial gene clusters. PmfE shares no similarity with any polypeptide in the SwissProt database. No regulatory gene(s) or regulatory elements were evident in the sequence. The pmf cluster shares common features with other enteric fimbrial gene clusters, but also displays features that are unique.
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201.
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Massad
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Mobley
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial; D010861:Fimbriae, Bacterial / Q000235:genetics*; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D011512:Proteus Infections / Q000382:microbiology; D011513:Proteus mirabilis / Q000235:genetics* / Q000472:pathogenicity
D004269:DNA, Bacterial