Preparation of antibody free spermatozoa by in vitro immunodepletion using immunobeads.
In the present study ejaculates from six infertile men with antisperm antibodies were processed on a six step discontinuous percoll gradient. This treatment yielded a sperm fraction with a different percentage of sperm with antisperm antibodies. The resultant sperm preparation was incubated with immunobeads. Sperm were recovered following incubation by swim-up. Sperm recovered at the end of processing showed reduced populations of antisperm antibodies. Even though sufficient numbers of sperm were obtained to consider assisted reproduction, reduced motility was recorded in four of the ejaculates when compared with routine processing. Following trial preparations of semen samples this technique may play a role in sperm selection for assisted conception.
Royal College of Surgeons, Rotunda Hospital, Dublin, Ireland.
M
M
Ryan
L
L
Drudy
E
E
Cottell
R F
RF
Harrison
D000906:Antibodies / Q000302:isolation & purification*; D001323:Autoantibodies / Q000302:isolation & purification*; D002469:Cell Separation / Q000379:methods; D002501:Centrifugation, Zonal / Q000379:methods; D006801:Humans; D008297:Male; D011205:Povidone; D012661:Semen / Q000276:immunology*; D012822:Silicon Dioxide; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology* / Q000276:immunology*
D000906:Antibodies; D001323:Autoantibodies; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone