Cloning and expression of a cDNA for rat prostaglandin F2 alpha receptor.
We have cloned a cDNA for rat prostaglandin (PG) F2 alpha receptor from cultured rat astrocytes. The cDNA encodes a polypeptide of 366 amino acids with seven putative transmembrane domains. Specific binding of [3H]PGF2 alpha in membranes of COS-7 cells transfected with the cDNA was displaced with unlabeled PGs in the order of PGF2 alpha > PGD2 > PGE2 > PGI2. In the cDNA-transfected LLC-PK1 cells, PGF2 alpha stimulated phosphoinositide hydrolysis. A significant 4.7-kb mRNA transcript was detected in cultured rat astrocytes and whole brain and pregnant ovary of adult rats by Northern blot analysis.
Kitanaka
J
J
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Hasimoto
H
H
Sugimoto
Y
Y
Negishi
M
M
Aino
H
H
Gotoh
M
M
Ichikawa
A
A
Baba
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D001483:Base Sequence; D001667:Binding, Competitive; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015237:Dinoprost / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008969:Molecular Sequence Data; D010716:Phosphatidylinositols / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011982:Receptors, Prostaglandin / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D010738:Type C Phospholipases / Q000378:metabolism
D018076:DNA, Complementary; D010716:Phosphatidylinositols; D011453:Prostaglandins; D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; D011994:Recombinant Proteins; C034671:prostaglandin F2alpha receptor; D015237:Dinoprost; D010738:Type C Phospholipases