Comparison of sensitivities of cells to X-ray therapy, chemotherapy, and isotope therapy using a tumor spheroid model.
Numerous treatment modalities have been used in treating microscopic carcinoma confined to the peritoneal cavity. However, there is little information at the cellular level regarding which modality is most effective in eradicating small nests of tumor cells. In order to determine whether chemotherapy, X rays, or isotope therapy is most effective, we have compared in a three-dimensional spheroid model the survival of cells after treatment with cis-platinum, X ray, or the investigational high-energy isotope 212-bismuth (Bi-212). In this study, V-79 cells were grown to 100-microns diameter spheroids. In killing spheroids, high-energy isotopes were at least six times more effective than X rays at fractionated doses of up to 1300 cGy. High-energy isotopes appear also to be as efficient as cis-platinum. Regardless of exposure time to treatment, Bi-212 was more toxic to spheroids than the other treatment modalities. From this study we conclude that high-energy isotope therapy biologically is very effective in eradicating microscopic nests of cells.
Department of Obstetrics and Gynecology, University of Chicago, Illinois 60637.
J
J
Rotmensch
J L
JL
Whitlock
S
S
Culbertson
R W
RW
Atcher
J L
JL
Schwartz
D000818:Animals; D001729:Bismuth / Q000592:standards* / Q000627:therapeutic use; D002945:Cisplatin / Q000592:standards* / Q000627:therapeutic use; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D010534:Peritoneal Neoplasms / Q000473:pathology* / Q000628:therapy*; D011868:Radioisotopes / Q000592:standards* / Q000627:therapeutic use; D011878:Radiotherapy / Q000592:standards*; D014407:Tumor Cells, Cultured
D011868:Radioisotopes; D002945:Cisplatin; D001729:Bismuth