Instant PRINS: a rapid method for chromosome identification by detecting repeated sequences in situ.
We describe a method for labeling specific chromosomes in situ by oligonucleotide-primed synthesis and incorporation of fluorochrome-labeled dUTP, together with an accelerated protocol for the reaction. The combination of the two developments produces extremely rapid results, in some cases taking less than 5 min for the complete process. The significance of this development for clinical diagnosis is discussed.
MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, UK.
J
J
Gosden
D
D
Lawson
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D002877:Chromosomes, Human; D002886:Chromosomes, Human, Pair 17; D002897:Chromosomes, Human, Pair 7; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry; D017931:DNA Primers; D004276:DNA, Satellite / Q000737:chemistry; D003856:Deoxyuracil Nucleotides / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D014998:Y Chromosome
D017931:DNA Primers; D004276:DNA, Satellite; D003856:Deoxyuracil Nucleotides; C027078:deoxyuridine triphosphate; D004247:DNA