[The differentiation of tumor cells of the human large intestine during the acquisition of multiple drug resistance].
By selection in the medium containing increasing actinomycin D concentrations two sublines with acquired multidrug resistance (MDR) caused by P-glycoprotein (P170) overproduction were isolated. The obtained cell lines as well as parent cells grow in vitro as morphologically organized aggregates, so-called organoids. Comparative electron microscopic study of sensitive and drug resistant organoids has shown that the development of MDR was accompanied by the enhancement of the tumour cell differentiation: the percentage of differentiated cells, the extent of their maturity, and the quantity of lumens were higher in MDR organoids than in parent cell line. The size of glandular structures in resistant organoids was also enlarged. Possible mechanisms of observed phenomenon are discussed.
N T
NT
Raĭkhlin
Iu L
IuL
Volodina
A G
AG
Perevoshchikov
B P
BP
Kopnin
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000037:antagonists & inhibitors*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000648:ultrastructure*; D018432:Drug Resistance, Multiple; D005455:Fluorescent Antibody Technique; D006801:Humans; D007078:Ileal Neoplasms / Q000378:metabolism / Q000648:ultrastructure*; D007080:Ileocecal Valve / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents
Differentsirovka kletok opukholeĭ tolstoĭ kishki cheloveka pri priobretenii mnozhestvennoĭ lekarstvennoĭ ustoĭchivosti.