Inhibition of prokaryotic and eukaryotic transcription by the 2:1 2,9-dimethyl-1,10-phenanthroline-cuprous complex, a ligand specific for open complexes.
The redox-stable, tetrahedral cuprous chelate of neocuproine (2,9-dimethyl-1,10-phenanthroline) binds to the single-stranded DNA formed in open complexes and is an effective inhibitor of eukaryotic and prokaryotic transcription. Despite the many kinetic and structural differences between prokaryotic and eukaryotic transcription systems, they are all similarly inhibited by neocuproine copper, suggesting that all open complexes may share a homologous structure.
Department of Biological Chemistry, School of Medicine, University of California at Los Angeles, 90024-1570.
D M
DM
Perrin
L
L
Pearson
A
A
Mazumder
D S
DS
Sigman
D001483:Base Sequence; D003300:Copper; D008024:Ligands; D008969:Molecular Sequence Data; D009942:Organometallic Compounds / Q000494:pharmacology* / Q000633:toxicity; D010618:Phenanthrolines / Q000494:pharmacology* / Q000633:toxicity; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016385:TATA Box; D014158:Transcription, Genetic / Q000187:drug effects*
D008024:Ligands; D009942:Organometallic Compounds; D010618:Phenanthrolines; C078638:copper-neocuproine complex; D003300:Copper