Repeated characterization of alcohol withdrawal reactions in rats chronically exposed to an alcohol liquid diet.
1. Rats given a 10-% (v/v) alcohol liquid diet over long periods of time reach high blood alcohol levels of more than 200 mg/dl over several weeks. 2. Repeated discontinuation of the alcohol intake resulted, each time within 8 hr, in several withdrawal reactions including a reduction in exploratory behaviour and tremorogenic activity. 3. The inhibition of exploratory activity was measured in a neutral two-chamber model, both in terms of the number of transits into the open area as well as the time spent in the open space. The differences in exploration remained over the 4 successive withdrawal tests. 4. Rats in alcohol withdrawal were also consistently less active than control animals in the tremor cages equipped with a piezofilm floor, and this despite the presence of a clearly visible tremor in the hindpaws when lifted up. 5. Alcohol withdrawal rats revealed a more frequent tremor activity than controls after a challenge with 5 mg/kg harmine. This effect was independent of the length of the tremor bursts used to quantify harmine-induced tremor starting from the second withdrawal period onwards. 6. With a dose of 10 mg/kg harmine, ceiling effects were reached in both the alcohol withdrawal and control rats and differences between the two groups were only present during the first exposures. 7. Overall, these results indicate that it is possible to quantify some withdrawal reactions at repeated time intervals of alcohol cessation in rats chronically exposed to a 10-% alcohol liquid diet. As a consequence, these withdrawal reactions can be studied in a more systematic way.
Department of Neuropsychopharmacology, Janssen Research Foundation, Beerse, Belgium.
T F
TF
Meert
H
H
Huysmans
D000437:Alcoholism / Q000523:psychology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004032:Diet; D000431:Ethanol / Q000008:administration & dosage / Q000097:blood; D005106:Exploratory Behavior / Q000187:drug effects; D006247:Harmine / Q000494:pharmacology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013375:Substance Withdrawal Syndrome / Q000523:psychology*; D014202:Tremor / Q000139:chemically induced
D000431:Ethanol; D006247:Harmine