Hepatic metabolism of chloral hydrate to free radical(s) and induction of lipid peroxidation.
Metabolism of chloral hydrate by male B6C3F1 mouse liver microsomes generates free radical intermediate(s) as evidenced by electron spin resonance spectroscopic analysis. The subsequent induction of endogenous lipid peroxidation was shown by analysis of the resulting products with high-pressure liquid chromatography. Chloral hydrate was found mutagenic in Salmonella typhimurium strain TA104. Both lipid peroxidation and mutagenicity were efficiently inhibited by free radical scavengers, alpha-tocopherol and menadione.
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National Center for Toxicological Research, Jefferson, AR 72079.
Wong
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Kadlubar
F F
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Fu
P P
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D000818:Animals; D001711:Biotransformation; D002697:Chloral Hydrate / Q000493:pharmacokinetics*; D016166:Free Radical Scavengers; D005609:Free Radicals; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D009153:Mutagens / Q000493:pharmacokinetics; D012486:Salmonella typhimurium / Q000235:genetics; D014810:Vitamin E / Q000494:pharmacology; D014812:Vitamin K / Q000494:pharmacology
D016166:Free Radical Scavengers; D005609:Free Radicals; D009153:Mutagens; D014812:Vitamin K; D014810:Vitamin E; D002697:Chloral Hydrate