Effect of diabetes, insulin, and glucose load on lipid peroxidation in the rat.
Lipid peroxidative activity in rats made diabetic with streptozocin and rats made acutely hyperglycemic by intraperitoneal dextrose administration was determined by measurement of exhaled ethane during exposure in vivo to ethane-free air (EFA). Diabetic rats demonstrated increased ethane in the expired breath while breathing EFA (5.82 +/- 0.56 pmol/min/100 g) compared with control rats (4.02 +/- 0.23 pmol/min/100 g). Insulin treatment of diabetic rats attenuated the ethane produced (4.88 +/- 0.23 pmol/min/100 g). Acute hyperglycemia increased exhaled ethane to levels higher than those seen in diabetic rats (9.87 +/- 0.98 pmol/min/100 g). Saline injected intraperitoneally to control rats produced ethane levels similar to those of untreated nondiabetic controls (4.11 +/- 0.52 pmol/min/100 g). Chronic uncontrolled hyperglycemia and acute hyperglycemia are associated with increased in vivo ethane production.
Tucson Veterans Affairs Medical Center, AZ 85723.
M P
MP
Habib
F D
FD
Dickerson
A D
AD
Mooradian
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001985:Bronchial Provocation Tests; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004980:Ethane / Q000378:metabolism; D005947:Glucose / Q000633:toxicity; D006943:Hyperglycemia / Q000139:chemically induced / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
D001786:Blood Glucose; D007328:Insulin; D005947:Glucose; D004980:Ethane