Diagnostic approach to autoimmune blistering diseases using immunoblotting with chemiluminescence.
Autoantibodies in sera from patients with autoimmune blistering diseases were detected by immunoblotting with chemiluminescence. This method provided the high sensitivity in the detection of autoantibodies against human epidermal extract. It was useful for detecting low titers of autoantibodies, and identifying small amounts of autoantigens in antigen extracts.
Department of Dermatology, Aichi Medical University, Japan.
T
T
Kitamura
Y
Y
Tamada
H
H
Takama
K
K
Yokochi
Y
Y
Nitta
T
T
Ikeya
D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000175:diagnosis*; D006801:Humans; D015151:Immunoblotting; D008163:Luminescent Measurements; D010391:Pemphigoid, Bullous / Q000175:diagnosis*; D010392:Pemphigus / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D001323:Autoantibodies