Recovery of duplications by drug resistance selection in Caenorhabditis elegans.
We have devised a scheme that facilitates rapid screening for duplications of essential loci. Our scheme takes advantage of the lev-1(x22) mutation, which confers resistance in a recessive fashion to the potent anthelmintic levamisole. We have tested our methodology by recovering two gamma ray induced duplications of let-56, the first essential gene to the left of unc-22. One of the duplications is attached to the fourth chromosome. The other duplication is attached to the X chromosome. This duplication contains a functional copy of the unc-22 gene, as well as functional copies of several essential loci adjacent to unc-22. Results we have obtained during analysis of this duplication are compatible with the notion that the copy of the unc-22 gene located on the duplication is subject to X chromosome dosage compensation.
Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.
M A
MA
Marra
D L
DL
Baillie
D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000235:genetics*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004303:Dosage Compensation, Genetic; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D017238:Genes, Helminth; D007978:Levamisole / Q000494:pharmacology; D008297:Male; D005810:Multigene Family; D016296:Mutagenesis; D014960:X Chromosome
D007978:Levamisole