Stimulation of macrophage oxygen free radical production and lymphocyte blastogenic response by immunization with porins.
Porins were prepared from smooth strain of Salmonella typhi 0-901 and chemotype rough mutant of S. typhimurium Ra-30. Porins could significantly stimulate the immune systems of mice. Immunization of mice with the porins provoked synthesis of anti-porin antibodies. Macrophages from the immunized mice showed increased capacity to generate oxygen free radicals, and lymphocytes from these mice showed proliferative response to the porins. Thus porins may play a role in providing protection from salmonellosis by stimulating the antibody production and increasing the capacity of macrophages to generate oxygen free radicals along with stimulation of lymphocytes.
Tabaraie
B
B
Department of Experimental Medicine, Postgraduate Institute of Medical Education & Research, Chandigarh, India.
Sharma
B K
BK
Sharma
P R
PR
Sehgal
R
R
Ganguly
N K
NK
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D008163:Luminescent Measurements; D008213:Lymphocyte Activation; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D010100:Oxygen / Q000378:metabolism; D018272:Porins / Q000276:immunology*; D012475:Salmonella / Q000276:immunology*; D012485:Salmonella typhi / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology; D014611:Vaccination
D000907:Antibodies, Bacterial; D005609:Free Radicals; D018272:Porins; D010100:Oxygen