Dentin permeability to bacterial proteins in vitro.
Passage of bacterial components through dentin is a subject of recent research with in vitro as well as with in vivo models. Diffusive transport of Porphyromonas gingivalis ATCC 33277 proteins has been demonstrated by the authors with an in vitro setup closely simulating the pulp chamber. The purpose of this investigation was to study the filtration of these proteins through dentin, measure possible concentration changes resulting from the filtering, and elaborate on the physical aspects of the binding process. The hydraulic conductance (Lp) of 10 dentin specimens was determined in three experiments using standard procedures: initially with phosphate-buffered saline, subsequently with a P. gingivalis ATCC 33277 suspension (200 micrograms/ml of protein), and finally with phosphate-buffered saline. The results showed significant, nonpermanent, alterations of the Lps of the dentin disks, and variable retention of the bacterial proteins among samples. A positive correlation between the bacterial protein retention and reduction of Lps was also demonstrated. The reduction of the Lps of the dentin samples was attributed to microstructural changes of the dentin, while the bacterial protein retention was considered to take place either on the surface of the disk or intratubularly. The intratubular interactions in the present experiment seemed to have a mechanical rather than a chemical basis.
Pissiotis
E
E
Department of Restorative Dentistry and Endodontology, University of Connecticut Health Center, School of Dental Medicine, Farmington.
Spångberg
L S
LS
D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport; D003806:Dentin Permeability; D004058:Diffusion; D006801:Humans; D006874:Hydrostatic Pressure; D008954:Models, Biological; D016966:Porphyromonas gingivalis
D001426:Bacterial Proteins