Bovine ENA, a new monocyte-macrophage derived cytokine of the interleukin-8 family. Structure, function, and expression in acute pulmonary inflammation.
A novel bovine neutrophil-activating peptide, bovine ENA (boENA), was identified in the conditioned media of endotoxin-stimulated bovine monocytes and alveolar macrophages. The chemotactic peptide was purified to homogeneity from conditioned media by cation-exchange chromatography and several steps of reversed-phase high-performance liquid chromatography. The partial amino acid sequence of boENA was: VVRELRCVCLTTTPGIHPKTVSDLQVIAAGPVCSKVEVIATLKNGXXV. Its cysteine molecules are positioned identically to those of the C-X-C family of human proinflammatory peptides. BoENA shows structural (73% identity in amino acid sequence) and functional homology to human ENA-78, a product of the human type II epithelial cell line A549, as demonstrated in assays for chemotaxis, aggregation, shape change, and a rise in intracellular free calcium. The immunohistochemical identification of boENA in the hyperplastic type II alveolar epithelial cells and in pulmonary alveolar leukocytes of pneumonic bovine lungs strongly supports a role for ENA-78 in the genesis of pulmonary inflammation.
Allmann-Iselin
I
I
Institute of Veterinary Pathology, University of Bern, Switzerland.
Car
B D
BD
Zwahlen
R D
RD
Mueller-Schüpbach
R
R
Wyder-Walther
M
M
Steckholzer
U
U
Walz
A
A
D000208:Acute Disease; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle / Q000097:blood; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000031:analogs & derivatives* / Q000145:classification / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008264:Macrophages / Q000378:metabolism*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism*; D011014:Pneumonia / Q000378:metabolism; D013329:Structure-Activity Relationship
D016207:Cytokines; D016209:Interleukin-8; C090781:endothelial neutrophil activating peptide