Expression of HLA-class II genes of IDDM patients on the surface of the LTK- cells.
To investigate the function of HLA-class II genes in the autoimmune response of insulin dependent diabetes mellitus (IDDM), the HLA-class II gene of IDDM patients was introduced into Ltk- cells with pSV2-neo plasmid, using the calcium phosphate precipitation technique. We obtained a stable cell line expressing the HLA-class II gene from lymphocytes of IDDM patients. Expression was identified by direct ox erythrocyte-CrCl3-HLA DR monoclonal antibody rosetting.
Wang
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H
PUMC Hospital, Beijing.
Sun
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Q
D000818:Animals; D002460:Cell Line; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005347:Fibroblasts / Q000166:cytology; D005802:Genes, MHC Class II; D006801:Humans; D051379:Mice; D010957:Plasmids; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D014162:Transfection
D011505:Protein-Tyrosine Kinases