title: Bryonolic acid production in hairy roots of Trichosanthes kirilowii Max. var Japonica Kitam. Transformed with Agrobacterium rhizogenes and its cytotoxic activity.
abstract: The hairy roots of Trichosanthes kirilowii Max, var. japonica Kitam. were induced by Agrobacterium rhizogenes (TCC 15834) on sterile shoots. The axenic culture of hairy roots proliferated 30 to 60-fold based on the initial fresh weight after three weeks of culture in Murashige & Skoog liquid media. Bryonolic acid as the main triterpenoid was isolated in a high yield, together with chondrillasterol from the hairy roots of this plant. Bryonolic acid showed strong inhibition of the growth of B-16 melanoma cells.
authors.affiliation: Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takeda
authors.forename: T
authors.initials: T
authors.lastname: Kondo
authors.forename: H
authors.initials: H
authors.lastname: Mizukami
authors.forename: Y
authors.initials: Y
authors.lastname: Ogihara
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D008516:Medicine, Chinese Traditional; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000503:physiopathology; D010946:Plants, Medicinal / Q000378:metabolism*; D012231:Rhizobium / Q000378:metabolism*; D014315:Triterpenes / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes; C074984:bryonolic acid