title: Structural heterogeneity of Pseudomonas aeruginosa bacterioferritin.
abstract: The subunit composition, amino acid sequence and haem-binding characteristics of bacterioferritin (BFR) from Pseudomonas aeruginosa have been studied. Unlike other BFRs, P. aeruginosa BFR was found to contain two subunit types, designated alpha and beta, which differed considerably in their amino acid sequences. The N-terminal 69 and 55 amino acids of the alpha and beta subunits respectively were determined. The alpha subunit differed most from other BFRs. The two subunits were present in variable proportions in different preparations. The maximum stoichiometry of haem binding was found to be sample-dependent and to be different from the previously reported one per subunit [Kadir and Moore (1990) FEBS Lett. 271, 141-143]. This previous haem-binding study was shown to have been carried out with damaged protein, which contained both normal alpha and beta subunits and shorter versions of these that appeared to have been produced by cleavage of the normal subunits. The possibility that aging processes degrade ferritins and affect their haem-binding characteristics is discussed.
authors.affiliation: Centre for Metalloprotein Spectroscopy and Biology, School of Chemical, University of East Anglia, Norwich, U.K.
authors.forename: G R
authors.initials: GR
authors.lastname: Moore
authors.forename: F H
authors.initials: FH
authors.lastname: Kadir
authors.forename: F K
authors.initials: FK
authors.lastname: al-Massad
authors.forename: N E
authors.initials: NE
authors.lastname: Le Brun
authors.forename: A J
authors.initials: AJ
authors.lastname: Thomson
authors.forename: C
authors.initials: C
authors.lastname: Greenwood
authors.forename: J N
authors.initials: JN
authors.lastname: Keen
authors.forename: J B
authors.initials: JB
authors.lastname: Findlay
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003573:Cytochrome b Group / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005293:Ferritins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006418:Heme / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D017421:Sequence Analysis
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D046911:Macromolecular Substances; D006418:Heme; D005293:Ferritins; C018032:bacterioferritin