title: [Cloning transport protein genes for two strains of tobacco mosaic virus and their expression in Escherichia coli cells].
abstract: Recombinant constructs expressing fusion (His)6 movement proteins of two strains (Cruciferous and Vulgare) of tobacco mosaic virus were generated by cloning the PCR-amplified fragments into the pQE-9 vector. The movement proteins containing N-terminal (His)6 affinity tags were purified on a metal chelate adsorbent.
authors.forename: K I
authors.initials: KI
authors.lastname: Ivanov
authors.forename: P A
authors.initials: PA
authors.lastname: Ivanov
authors.forename: E K
authors.initials: EK
authors.lastname: Timofeeva
authors.forename: V A
authors.initials: VA
authors.lastname: Efimov
authors.forename: Iu L
authors.initials: IuL
authors.lastname: Dorokhov
mesh_terms: D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D002352:Carrier Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins
vernacular_title: Klonirovanie genov transportnykh belkov dvukh shtammov virusa tabachnoĭ mozaiki i ikh ékspressiia v kletkakh Escherichia coli.