A long polypyrimidine:polypurine sequence in 5' flanking region of arylsulfatase gene of sea urchin embryo.
Sea urchin (Hemicentrotus pulcherrimus) arylsulfatase(Ars) gene contains a long (622 bp) polypyrimidine:polypurine (Pyr-Pur) sequence in its 5' flanking region. The Pyr-Pur sequence inserted into a plasmid was sensitive to S1 nuclease at a low acidic pH (pH 5) when the plasmid was negatively supercoiled. From the distribution pattern of S1 sites in the Pyr-Pur region it is concluded that a (CT)11:(GA)11 tract in this region could adopt an unusual DNA configuration distinct from the usual B-form. Another feature of the Pyr-Pur sequence is that this (CT)11:(GA)11 tract is sandwiched by two oligo(dC):oligo(dG) stretches (G-strings) that are located at almost an equal distance from both ends of the (CT)11:(GA)11 tract. Mobility shift assay and DNase-I footprinting revealed that the gastrula nuclei contain nuclear proteins that interact with two distinct oligo(dG):oligo(dG) tracts (G-strings) in the Pyr-Pur region. The possibility is suggested that G-strings may be related to formation and stabilization of an unusual DNA configuration of a (CT)11:(GA)11 tract.
Yamamoto
T
T
Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Japan.
Akasaka
K
K
Irie
S
S
Shimada
H
H
D000818:Animals; D001192:Arylsulfatases / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012617:Sea Urchins / Q000196:embryology / Q000201:enzymology / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases
D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases; D001192:Arylsulfatases