Effect of purified IgGs from HIV-1-infected and non infected individuals on immune activation.
The purification and analysis of IgGs from sera of HIV-1-infected and non infected individuals are reported. The effect of antibodies purified from sera of infected individuals on antigen-induced T cell proliferation was investigated in relation to their possible involvement in an autoimmune reaction in AIDS, in view of the previously unravelled striking peptide similarities between HIV-1 gp120 and the immunoregulatory CD4 and Fas molecules. However, our data do not allow definite conclusions to be drawn. The necessity of purifying antibodies against specific peptides to show their direct effect on T-cell activation is further stressed.
Chams
V
V
Laboratoire de Physiologie Cellulaire, Université P & M Curie, Paris, France.
Biou
D
D
Cho
Y Y
YY
Mbika
J P
JP
Le Coq
H
H
Heshmati
F
F
Fouchard
M
M
Bizzini
B
B
Zagury
J F
JF
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015551:Autoimmunity; D015704:CD4 Antigens / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D018023:HIV Seronegativity / Q000276:immunology; D015497:HIV-1; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification
D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D007074:Immunoglobulin G