title: Contribution of a H+ pump in determining the resting potential of neuroblastoma cells.
abstract: The aim of this work was to examine the effects of changes in external K+ concentration (Ko) around its physiological value, of various K+ channels blockers, including internal Cs+, of vacuolar H(+)-ATPase inhibitors and of the protonophore CCCP on the resting potential and the voltage-dependent K+ current of differentiated neuroblastoma x glioma hybrid NG108-15 cells using the whole-cell patch-clamp technique. The results are as follows: (i) under standard conditions (Ko = 5 mM) the membrane potential was -60 +/- 1 mV. It was unchanged when Ko was decreased to 1 mM and was depolarized by 4 +/- 1 mV when Ko was increased to 10 mM. (ii) Internal Cs+ depolarized the membrane by 21 +/- 3 mV. (iii) The internal application of the vacuolar H(+)-ATPase inhibitors N-ethylmaleimide (NEM), NO3- and bafilomycin A1 (BFA) depolarized the membrane by 15 +/- 2, 18 +/- 2 and 16 +/- 2 mV, respectively. (iv) When NEM or BFA were added to the internal medium containing Cs+, the membrane was depolarized by 45 +/- 1 and 42 +/- 2 mV, respectively. (v) The external application of CCCP induced a transient depolarization followed by a prolonged hyperpolarization. This hyperpolarization was absent in BFA-treated cells. The voltage-dependent K+ current was increased at negative voltages and decreased at positive voltages by NEM, BFA and CCCP. Taken together, these results suggest that under physiological conditions, the resting potential of NG108-15 neuroblastoma cells is maintained at negative values by both voltage-dependent K+ channels and an electrogenic vacuolar type H(+)-ATPase.
authors.affiliation: Laboratoire de Physiologie Cellulaire, URA CNRS 1121, Université Paris-Sud, Orsay, France.
authors.forename: V
authors.initials: V
authors.lastname: Gérard
authors.forename: B
authors.initials: B
authors.lastname: Rouzaire-Dubois
authors.forename: J M
authors.initials: JM
authors.lastname: Dubois
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002586:Cesium / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005910:Glioma / Q000473:pathology* / Q000503:physiopathology* / Q000648:ultrastructure; D018942:Macrolides; D008564:Membrane Potentials / Q000502:physiology*; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology* / Q000503:physiopathology* / Q000648:ultrastructure; D009573:Nitrites / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D015221:Potassium Channels / Q000502:physiology; D017494:Proton Pumps / Q000502:physiology*; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D018942:Macrolides; D009573:Nitrites; D015221:Potassium Channels; D017494:Proton Pumps; D002586:Cesium; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D010042:Ouabain; C040929:bafilomycin A1; D005033:Ethylmaleimide; D011188:Potassium