[In situ PCR-gene hybridization method for detecting mycobacterium tuberculosis in tissue specimens].
The authors combined the technique of polymerase chain reaction (PCR) with hybridization in situ, and find the method is better, quicker and more sensitive for detecting a trace of bacterial DNA, especially for observing the relation between the lesion and the peripheral tissue, the method is more simple and no radioactive pollution. It seems to be suitable for detection on samples from autopsy, biopsy and smears. We suppose the method might have a prospect for clinical diagnosis.
Hu
M
M
Department of Pathology, Fourth Military Medical University, Xi'an.
Wang
W L
WL
Zhao
Y L
YL
D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D017403:In Situ Hybridization; D008168:Lung / Q000382:microbiology*; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D004269:DNA, Bacterial