title: Identification of potential hot spots in the carboxy-terminal part of the Epstein-Barr virus (EBV) BNLF-1 gene in both malignant and benign EBV-associated diseases: high frequency of a 30-bp deletion in Malaysian and Danish peripheral T-cell lymphomas.
abstract: In this study, we have sequenced the C-terminal part of the Epstein-Barr virus (EBV)-BNLF-1 gene encoding for the latent membrane protein-1 from tissues of EBV-positive Danish Hodgkin's disease (HD) and of Danish and Malaysian peripheral T-cell lymphomas (PTLs) and from tonsils of Danish infectious mononucleosis (IM). Our study showed that some of the 7 single-base mutations and the 30-bp deletion previously detected between codons of amino acid 322 and 366 in the BNLF-1 gene of the nasopharyngeal carcinoma cell line CAO were present in all Malaysian PTLs and in 60% of the Danish PTLs. In HD and the IM cases, the mutations were present in about 30%. The 30-bp deletion and the single base mutations occurred independently, and mutations were detectable in the majority of EBV type B-positive cases. These findings suggest that the 30-bp deletion and the 7 single-base mutations in the C-terminal part of the CAO-BNLF-1 gene do not characterize a new EBV type A substrain. Rather, some of the positions of single base mutations and the 30-bp deletion are hot spots that may have mutated independently through the evolution of EBV strains.
authors.affiliation: Department of Pathology, Aarhus University Hospital, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Sandvej
authors.forename: S C
authors.initials: SC
authors.lastname: Peh
authors.forename: B S
authors.initials: BS
authors.lastname: Andresen
authors.forename: G
authors.initials: G
authors.lastname: Pallesen
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D003718:Denmark; D015967:Gene Expression Regulation, Viral; D005787:Gene Frequency; D005814:Genes, Viral; D006566:Herpesviridae Infections / Q000382:microbiology*; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006689:Hodgkin Disease / Q000382:microbiology*; D006801:Humans; D007244:Infectious Mononucleosis / Q000382:microbiology*; D016411:Lymphoma, T-Cell, Peripheral / Q000382:microbiology*; D008296:Malaysia; D008969:Molecular Sequence Data; D017384:Sequence Deletion; D014412:Tumor Virus Infections / Q000382:microbiology*; D014763:Viral Matrix Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D004273:DNA, Neoplasm; D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins