title: Swiss population data on three tetrameric short tandem repeat loci--VWA, HUMTHO1, and F13A1--derived using multiplex PCR and laser fluorescence detection.
abstract: Allele and genotype frequencies for 3 tetrameric short tandem repeat loci VWA, HUMTHO1, and F13A1 were determined in a Swiss population sample using multiplex PCR and subsequent electrophoresis in DNA sequencing gels processed by automated laser fluorescence detection. The technique allows single base pair resolution and rapid typing, with a concomitant reduction in the potential for human transcriptional typing errors. All loci meet Hardy-Weinberg expectations. In addition, there is little evidence for association of alleles among the 3 loci. The allelic frequency data can be used in forensic analyses and paternity tests to estimate the frequency of a multiple STR locus DNA profile in the Swiss population.
authors.affiliation: Department of Forensic Medicine, University of Bern, Switzerland.
authors.forename: M N
authors.initials: MN
authors.lastname: Hochmeister
authors.forename: J M
authors.initials: JM
authors.lastname: Jung
authors.forename: B
authors.initials: B
authors.lastname: Budowle
authors.forename: U V
authors.initials: UV
authors.lastname: Borer
authors.forename: R
authors.initials: R
authors.lastname: Dirnhofer
mesh_terms: D000483:Alleles; D000941:Antigens / Q000235:genetics; D002874:Chromosome Mapping; D016172:DNA Fingerprinting / Q000379:methods*; D005176:Factor XIII / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D005828:Genetics, Population; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D013557:Switzerland; D014841:von Willebrand Factor / Q000276:immunology
chemical_list: D000941:Antigens; D005819:Genetic Markers; C029417:Von Willebrand antigen; D014841:von Willebrand Factor; C064015:factor XIII subunit A; D005176:Factor XIII