title: Development of epileptic activity in embryos and newly hatched chicks of the Fayoumi mutant chicken.
abstract: The homozygous Fayoumi strain of epileptic chickens (Fepi) is affected by generalized convulsions consistently induced by intermittent light stimulation (ILS) and by intense sound. Although interictal EEG recordings show continuous spikes and spike and wave activity, desynchronization and flattening (DF) of the EEG are observed during seizures. We have studied development of the epileptic phenotype in embryonic (E) and posthatching (P) Fepi. As compared with those of chicken embryos of a normal strain, no differences were observed in the EEG before embryonic day (E) 16. Clearly differentiated spikes and spike and waves appeared at E17 in Fepi. Metrazol-induced EEG seizures were observed at E16 in normal embryos and at E17 in Fepi. The Fepi showed some characteristics: Spontaneous EEG seizure-like discharges also appeared at E17 but decreased toward hatching; visual or acoustic hyperexcitability developed at E20 together with evoked responses in normal chickens; desynchronization of the EEG, typical of the epileptic seizure of the adult, could be induced by ILS at B20, but ILS- or sound-induced generalized motor seizures appeared at P1, a few hours after hatching. Results show that Fepi phenotype reaches full expression at P1, but the electric paroxysms are expressed earlier, paralleling synaptic maturation.
authors.affiliation: Laboratoire de Physiologie de la Motricité, CNRS-UR 14, Université Pierre et Marie Curie, CHU Pitié-Salpetrière, Paris, France.
authors.forename: N T
authors.initials: NT
authors.lastname: Guy
authors.forename: N
authors.initials: N
authors.lastname: Fadlallah
authors.forename: R
authors.initials: R
authors.lastname: Naquet
authors.forename: C
authors.initials: C
authors.lastname: Batini
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000235:genetics*; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D004195:Disease Models, Animal; D004569:Electroencephalography; D004827:Epilepsy / Q000175:diagnosis / Q000196:embryology / Q000235:genetics*; D005315:Fetal Diseases / Q000175:diagnosis / Q000235:genetics