Cloning and expression of the gene encoding the 7Fe type ferredoxin from a thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii.
The structural gene (fdxA) coding for a 7Fe type ferredoxin of a thermophilic hydrogen oxidizing bacterium Bacillus schlegelii has been cloned and sequenced. The fdxA coding region is 231 nucleotides which codes for a 77 amino acids protein with the molecular weight of 8,744 except for iron-sulfur clusters. The fdxA gene has been expressed in E. coli to obtain the recombinant ferredoxin. The recombinant ferredoxin have shown the identical properties to the native one for absorption spectra, and SDS-PAGE.
Aono
S
S
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Nakamura
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S
Aono
R
R
Okura
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I
D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D005288:Ferredoxins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism; D007501:Iron / Q000032:analysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D015183:Restriction Mapping; D013053:Spectrophotometry
D017931:DNA Primers; D004269:DNA, Bacterial; D005288:Ferredoxins; D011994:Recombinant Proteins; D006859:Hydrogen; D007501:Iron