Western blot analysis of thyrotropin receptor expression in human thyroid tumours and correlation with TSH-binding.
Expression of thyrotropin receptor (TSHr) was analyzed in normal human thyroids and differentiated thyroid tumours by Northern blotting of total RNA and by Western blotting of detergent-solubilized membrane proteins with an antibody specific for the extracellular domain of TSHr. Under nonreducing conditions TSHr stained as a 90-kDa protein in normal thyroid tissue. Expression varied in differentiated carcinomas from normal to not detectable in parallel to steady state TSHr mRNA levels and TSH binding in a TSH binding assay. The negative expression of TSHr in some patients with differentiated carcinomas may have consequences for the TSH suppressive treatments of these patients.
Pötter
E
E
Department of Clinical Endocrinology, Medizinische Hochschule Hannover, Germany.
Horn
R
R
Scheumann
G F
GF
Dralle
H
H
Costagliola
S
S
Ludgate
M
M
Vassart
G
G
Dumont
J E
JE
Brabant
G
G
D001665:Binding Sites; D015153:Blotting, Western; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011989:Receptors, Thyrotropin / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000378:metabolism*; D013972:Thyrotropin / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D011989:Receptors, Thyrotropin; D013972:Thyrotropin