title: Design of nasoseptal cartilage replacements synthesized from biodegradable polymers and chondrocytes.
abstract: Reconstructive and aesthetic surgery of the nose is a challenging problem in facial plastic surgery. In this study, biodegradable polymers composed of polyglycolic acid (PGA) and poly-L-lactic acid (PLLA) and their co-polymers were used to produce templates to transplant cells and promote regeneration of structural cartilage. A highly porous anatomically shaped three-dimensional non-woven PGA fibre network was sprayed with a coating polymer solution. Reinforcement of the outer circumference of the 12 nasoseptal constructs using high molecular weight PLLA further stabilized the constructs during the process of neomorphogenesis of cartilage, both during in vitro incubation and in vivo implantation. These cell transplantation devices also proved to be adhesive substrates for dissociated bovine chondrocytes. When implanted subcutaneously into nude mice, the polymer templates guided the reorganization after 8 wk of the bovine chondrocytes into neocartilage in the precisely designed size and shape of the original size and shape of the polymer delivery device. All implants loaded with chondrocytes showed evidence of formation of histologically organized hyaline cartilage. The implantation of nasal scaffolds without cells did not show cartilage formation. The technique of tissue engineered growth of cartilage has potential applications in orthopaedic, plastic and reconstructive, and craniomaxillofacial surgery.
authors.affiliation: Laboratory for Transplantation and Tissue Engineering, Children's Hospital, Medical Center, Boston, MA 02115.
authors.forename: W C
authors.initials: WC
authors.lastname: Puelacher
authors.forename: D
authors.initials: D
authors.lastname: Mooney
authors.forename: R
authors.initials: R
authors.lastname: Langer
authors.forename: J
authors.initials: J
authors.lastname: Upton
authors.forename: J P
authors.initials: JP
authors.lastname: Vacanti
authors.forename: C A
authors.initials: CA
authors.lastname: Vacanti
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001698:Biomedical Engineering; D002417:Cattle; D017690:Cell Transplantation; D006801:Humans; D007773:Lactates; D019344:Lactic Acid; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D009300:Nasal Septum / Q000166:cytology / Q000601:surgery*; D011091:Polyesters; D011100:Polyglycolic Acid; D011108:Polymers; D011474:Prosthesis Design
chemical_list: D001672:Biocompatible Materials; D007773:Lactates; D011091:Polyesters; D011108:Polymers; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide)