title: Nutritional upshift response of ribosomal protein gene transcription in Saccharomyces cerevisiae.
abstract: Switching Saccharomyces cerevisiae from non-fermentative to fermentative growth by adding glucose to a medium with glycerol as the sole carbon source, leads to a sudden increase in the rate of ribosomal protein gene transcription. By analyzing the nutritional shift response in a variety of yeast mutants and in the presence of different drugs, evidence was obtained that: (i) no de novo protein synthesis is required for this response; (ii) protein kinase A is essential, though independent of intracellular levels of cAMP, whereas protein kinase C is not involved; (iii) proper regulation of sugar phosphorylation is essential; (iv) glycolysis is required for the long term effect of the nutritional upshift; and (v) pathways leading to glucose-induced activation differ from those leading to gene repression, probably already at the level of glucose transport.
authors.affiliation: Department of Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: G
authors.initials: G
authors.lastname: Griffioen
authors.forename: W H
authors.initials: WH
authors.lastname: Mager
authors.forename: R J
authors.initials: RJ
authors.lastname: Planta
mesh_terms: D000242:Cyclic AMP / Q000096:biosynthesis; D017868:Cyclic AMP-Dependent Protein Kinases / Q000096:biosynthesis; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D008954:Models, Biological; D011493:Protein Kinase C / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D015398:Signal Transduction; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D012333:RNA, Messenger; D012269:Ribosomal Proteins; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D005947:Glucose; D005990:Glycerol