[The influence of different cryoprotectant buffers on the fertilizing ability of human sperm "in vitro". I. The choice of cryoprotective buffers and methods of freezing].
We attempted to find the optimal way of cryopreservation of male gametes. We have been tested 9 cryoprotectants currently existing in the world literature. These fluids have been so far separately investigated in different centers of infertility treatment. In this study we compared the effectivity of these fluids in preservation of viable sperm. Basic seminological parameters and two variants of freezing were tested. There were noted significant differences between fast and slow cooling of sperm samples. Nevertheless after fast cooling we found a marked decrease of seminological parameters in preserved sperm when compared to fresh samples.
Jedrzejczak
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Kliniki Rozrodczości Instytutu Ginekologii i Połoznictwa AM w Poznaniu, Kierownik Kliniki.
Pisarski
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Kurpisz
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D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D011044:Poland; D013074:Sperm Banks / Q000379:methods; D013094:Spermatozoa / Q000187:drug effects
D003451:Cryoprotective Agents
Wpływ płynów krioprotekcyjnych na zdolność zapładniajaca plemników "in vitro". I. Wybór buforów protekcyjnych i sposobu mrozenia.