The presence of tumor necrosis factor-alpha and its antibody in the sera of cachexic patients with gastrointestinal cancer.
Although cancer cachexia has been shown to involve several cytokines, the tumor necrosis factor-alpha (TNF) has rarely been detected in such patients. In this study, sera from 21 patients with cancer cachexia were examined for the presence of TNF and the anti-TNF antibody using an enzyme-linked immunosorbent assay (ELISA) and Western blotting, respectively. All of the patients had recurrent cancer and manifested the characteristics of progressive body weight loss. TNF was found in the sera of four patients (20%) at levels ranging from 10.4 to 53.1 pg/ml, while a positive reaction for the anti-TNF antibody was detected in the sera from six patients (30%), two of whom showed both TNF and its antibody. Thus, either TNF or the anti-TNF antibody was present in the sera from 8 of 21 patients (40%). The results of this study indicate that TNF may be present in the circulation of at least 40% of cachexic patients, and suggest that it may be one of the main mediators of cancer-associated cachexia.
Kiyama
T
T
First Department of Surgery, Nippon Medical School, Tokyo, Japan.
Onda
M
M
Tokunaga
A
A
Fujita
I
I
Okuda
T
T
Mizutani
T
T
Matsukura
N
N
Todome
Y
Y
Ohkuni
H
H
D000328:Adult; D000368:Aged; D000912:Antibodies, Neoplasm / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000302:isolation & purification; D015153:Blotting, Western; D002100:Cachexia / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000302:isolation & purification*
D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D014409:Tumor Necrosis Factor-alpha