title: Trisomy 12 is uncommon in typical chronic lymphocytic leukaemias.
abstract: The incidence of trisomy 12 was studied by conventional chromosome analysis in 111 patients referred as B-cell chronic lymphocytic leukaemia (B-CLL). Fluorescent in situ hybridization (FISH) was also applied in 34 of those patients with either a normal karyotype or no analysable mitoses. By karyotyping, trisomy 12 was present in 11.7% (13/111), whereas additional FISH increased the incidence to 14.4% (16/111). When subdividing our cases in either typical CLL (n = 90), fulfilling the FAB classification criteria, or atypical CLL (n = 21), with one or more variations from those criteria, the incidence of +12 by metaphase analysis was 3% and 48%, respectively. Additional FISH increased the incidence to 4% and 57%. The most common aberration in atypical CLL was FMC7 positivity (n = 11), followed by CD5 negativity (n = 8), strong surface immunoglobulin staining (n = 7) and atypical morphology (n = 6). Trisomy 12 could only be demonstrated in a small proportion of neoplastic cells in all positive cases. By FISH and/or karyotyping, all available samples at diagnosis of the disease were positive.
authors.affiliation: Department of Haematology, A.Z. Sint-Jan, Brugge, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Criel
authors.forename: I
authors.initials: I
authors.lastname: Wlodarska
authors.forename: P
authors.initials: P
authors.lastname: Meeus
authors.forename: M
authors.initials: M
authors.lastname: Stul
authors.forename: A
authors.initials: A
authors.lastname: Louwagie
authors.forename: A
authors.initials: A
authors.lastname: Van Hoof
authors.forename: M
authors.initials: M
authors.lastname: Hidajat
authors.forename: C
authors.initials: C
authors.lastname: Mecucci
authors.forename: H
authors.initials: H
authors.lastname: Van den Berghe
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002881:Chromosomes, Human, Pair 12; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D014314:Trisomy