The prothoracicotropic hormone (PTTH) of the commercial silkmoth, Bombyx mori, in the CNS of the tobacco hornworm, Manduca sexta.
Immunocytochemistry revealed that a Bombyx mori prothoracicotropic hormone (PTTH)-like peptide is expressed by the Manduca sexta big PTTH-producing neurons, the lateral neurosecretory cell group III (L-NSC III). Independent PCR of genomic DNA and a L-NSC III cDNA library yielded products with 99% sequence similarity to the cDNA encoding Bombyx PTTH. This similarity necessitated evaluation of the relationship between Manduca big PTTH and Bombyx PTTH by 1) bioassay of IEF separated Manduca PTTH and 2) direct assessment of Bombyx PTTH biological activity with Manduca prothoracic glands. Together, these studies indicate that Bombyx PTTH and Manduca PTTH are different peptides expressed by the L-NSC III. The possible physiological significance of a Bombyx PTTH-like peptide in Manduca and its coexpression with Manduca big PTTH by the L-NSC III are discussed.
Gray
R S
RS
Department of Biology, University of North Carolina at Chapel Hill 27599-3280.
Muehleisen
D P
DP
Katahira
E J
EJ
Bollenbacher
W E
WE
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000737:chemistry*; D002490:Central Nervous System / Q000737:chemistry* / Q000166:cytology; D007301:Insect Hormones / Q000302:isolation & purification*; D018402:Manduca / Q000737:chemistry*; D008969:Molecular Sequence Data; D009474:Neurons / Q000737:chemistry; D009479:Neuropeptides / Q000302:isolation & purification*; D009490:Neurosecretory Systems / Q000737:chemistry / Q000166:cytology; D012689:Sequence Homology, Nucleic Acid
D007301:Insect Hormones; D009479:Neuropeptides; C009089:prothoracicotropic hormone