Increased expression of angiotensin peptides in the brain of transgenic hypertensive rats.
We determined the levels of angiotensin I (ANG I), angiotensin II (ANG II), and the heptapeptide angiotensin(1-7) [ANG(1-7)] in the blood and brain of female Hannover Sprague-Dawley (SD) and transgenic hypertensive rats [mRen-2]27 by radioimmunoassay and high performance liquid chromatography. Hypertension was accompanied by higher plasma concentrations of ANG II, no statistical changes in ANG(1-7), and no differences in plasma ANG I levels. In the hypothalamus of transgenic rats, concentrations of ANG II and ANG(1-7) averaged 827% and 168% above values in SD rats (p < 0.005) whereas both ANG I and ANG II increased in the medulla oblongata. The data showed that the established phase of hypertension in rats harboring the mouse Ren-2 gene is associated with overexpression of the renin-angiotensin system in brain regions participating in the endocrine regulation of blood pressure.
Senanayake
P D
PD
Hypertension Center, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC 27105.
Moriguchi
A
A
Kumagai
H
H
Ganten
D
D
Ferrario
C M
CM
Brosnihan
K B
KB
D000803:Angiotensin I / Q000096:biosynthesis* / Q000097:blood; D000804:Angiotensin II / Q000096:biosynthesis* / Q000097:blood; D000818:Animals; D030801:Animals, Genetically Modified; D002540:Cerebral Cortex / Q000378:metabolism; D005260:Female; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism; D008526:Medulla Oblongata / Q000378:metabolism; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000235:genetics
D010446:Peptide Fragments; D000804:Angiotensin II; D000803:Angiotensin I; D012083:Renin; C118790:angiotensin I (1-7)