Molecular cloning and characterization of murine cyclin E.
Using a monoclonal antibody developed to a mouse trophectodermal carcinoma stem cell line E6496D lysate, we have identified the gene that encodes murine cyclin E. The cDNA contains a consensus cyclin box and a long 3' untranslated region and shares over 75% homology with human cyclin E cDNA. We show that the gene is expressed in fetal tissues, embryonal carcinoma F9 cells and yolk sac carcinoma PYS-2 cells, but is not expressed in preimplantation stages of development. In adult tissues, cycE mRNA is detectable in the spleen and to a lesser extent in the testis and brain. These data show that cycE is developmentally regulated and unevenly expressed in fetal and adult mouse tissues.
Damjanov
I
I
Department of Pathology and Cell Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107.
Shan
J
J
Wang
R F
RF
Damjanov
A
A
DeLoia
J A
JA
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D018236:Carcinoma, Embryonal; D002460:Cell Line; D003001:Cloning, Molecular; D017124:Conserved Sequence; D016213:Cyclins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D005333:Fetus; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D016213:Cyclins; D011994:Recombinant Proteins; D004247:DNA