Characterization of a human locus in transition.
The spermatid-specific nucleoprotamine genes PRM1 and PRM2 and the transition protein gene TNP2 are clustered at a single site on human chromosome 16p13.2. To begin to understand the mechanism governing their genesis and coordinate regulation the primary sequence of this approximately 40.6 kilobase region was determined. This cluster of genes is embedded within a series of repetitive elements, including numerous Alu elements distributed at a frequency of > 1 Alu element/kilobase. Multiple Alu elements have integrated into separate truncated L1 sequences within this region. Many of these Alu elements are tandemly inserted or clustered. The role of repetitive elements in the genomic organization and evolution of this gene cluster is discussed. Computer-assisted sequence analysis revealed the presence of structural sequence elements often associated with the boundary regions of active transcriptional domains. Further analysis identified a CpG island at the 3' end of this segment of chromosome 16 and other candidate coding segments within this region indicative of an additional linked gene. These sequence landmarks are commensurate with the complexity of the region.
Nelson
J E
JE
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Krawetz
S A
SA
D001483:Base Sequence; D002868:Chromosomal Proteins, Non-Histone; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D011479:Protamines / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D011479:Protamines; C053886:spermatid transition proteins; D004247:DNA