Studies on the immunomodulatory effects of vitamin D.
The effect of vitamin D on T lymphocytes was investigated using the test of active E-rosettes formation. It was found that: 1. A single oral administration of 600,000 IU of vitamin D2 causes a significant increase in the formation of active E-rosettes. The peak of the increase was found 24 hours after the administration of vitamin D. The formation of E-rosettes the seventh day of the study was comparable with the pre-administration value; 2. Sera with higher concentration of 1,25-dihydroxyvitamin D[1,25(OH)2D] cause a significant increase in the formation of active E-rosettes; 3. Vitamin D is capable of reverting significantly the effect of a serum factor which suppresses the active E-rosettes formation. The authors observed a significant decrease in the effect of trypsin on the lymphocytes after oral administration of a single oral dose of 600,000 IU of vitamin D2. The trypsin-treated lymphocytes regained their lost capacity to bind sheep red blood cells (SRBC) when incubated with AB serum at 37 degrees C for 3 hours. The recovery of E-rosettes-forming capacity of trypsinised lymphocytes considerably increased when the cells were incubated with serum containing higher concentration of 1,25(OH)2D of 600,000 IU of vitamin D2.
Porsová-Dutoit
I
I
Department of Internal Medicine, 2nd Faculty of Medicine, Charles University, Praha, Czech Republic.
Blahos
J
J
Rovenský
J
J
Babický
A
A
Justová
V
V
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000284:Administration, Oral; D000328:Adult; D000368:Aged; D000818:Animals; D002117:Calcitriol / Q000097:blood; D002448:Cell Adhesion / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017698:Postmenopause; D012397:Rosette Formation; D012756:Sheep; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology; D014807:Vitamin D / Q000008:administration & dosage / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D014807:Vitamin D; D014357:Trypsin; D002117:Calcitriol