Potential forensic applications of minisatellite variant repeat (MVR) mapping using the polymerase chain reaction (PCR) at D1S8.
Minisatellite variant repeat (MVR) mapping using the polymerase chain reaction (PCR) at D1S8 (MS32) was applied to samples from various human tissues. All DNA samples obtained from an individual's organs at autopsy consistently gave the same digital diploid codes. Even 1 ng of genomic DNA was sufficient to obtain authentic diploid MVR coding ladders. MVR-PCR could be reliably applied to DNA isolated from bloodstains, saliva stains, seminal stains and plucked hair roots, and should become a powerful tool for individual identification in forensic investigations.
Yamamoto
T
T
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
Tamaki
K
K
Kojima
T
T
Uchihi
R
R
Katsumata
Y
Y
D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D004276:DNA, Satellite / Q000737:chemistry*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004276:DNA, Satellite