title: Toward cataloguing all rice genes: large-scale sequencing of randomly chosen rice cDNAs from a callus cDNA library.
abstract: A large-scale sequence analysis of rice cDNA was performed for a library from rice callus cultured in a medium containing 1 p.p.m. of 2,4-dichlorophenoxyacetic acid. Random sequencing of 2778 cDNA clones generated 2259 non-redundant expressed sequence tags (ESTs). The strategy of sequencing cDNAs can yield quickly a large number of novel genes. After translation, 690 sequences showed a significant amino acid sequence similarity to sequences already known from PIR. The source of known proteins ranged from bacteria to human. In this report, the non-redundant set of 280 identified ESTs is analyzed in detail.
authors.affiliation: Rice Genome Research Program, National Institute of Agrobiological Resources/Institute of Society for Technoinnovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: J
authors.initials: J
authors.lastname: Song
authors.forename: Y
authors.initials: Y
authors.lastname: Koga-Ban
authors.forename: E
authors.initials: E
authors.lastname: Matsui
authors.forename: F
authors.initials: F
authors.lastname: Fang
authors.forename: H
authors.initials: H
authors.lastname: Higo
authors.forename: H
authors.initials: H
authors.lastname: Nagasaki
authors.forename: M
authors.initials: M
authors.lastname: Hori
authors.forename: M
authors.initials: M
authors.lastname: Miya
authors.forename: E
authors.initials: E
authors.lastname: Murayama-Kayano
mesh_terms: D000818:Animals; D018076:DNA, Complementary; D004798:Enzymes / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D006801:Humans; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D004798:Enzymes; D010940:Plant Proteins