A method for increasing the concentration of a specific internal metabolite in steady-state systems.
An approach is described by which it is possible to increase the concentration of any internal metabolite without affecting the concentrations of other metabolites and fluxes in the organism. This approach requires the manipulation of only a limited number of enzyme activities. The method shows which enzymes to manipulate and the extent of the manipulation required to achieve a given increase in a chosen metabolite. A case study involving tryptophan overproduction in Saccharomyces cerevisae is given as a practical example of how this method could be used.
Small
J R
JR
Institute of Cell, Animal and Population Biology, University of Edinburgh, Scotland.
Kacser
H
H
D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000378:metabolism; D000878:Anthranilate Synthase / Q000378:metabolism; D002826:Chorismate Mutase / Q000378:metabolism; D004058:Diffusion; D005246:Feedback; D007700:Kinetics; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D014176:Protein Biosynthesis; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D000637:Transaminases / Q000378:metabolism; D014364:Tryptophan / Q000096:biosynthesis* / Q000378:metabolism; D014367:Tryptophan Synthase / Q000378:metabolism
D009243:NAD; D014364:Tryptophan; D010723:3-Deoxy-7-Phosphoheptulonate Synthase; D000637:Transaminases; D000878:Anthranilate Synthase; D014367:Tryptophan Synthase; D002826:Chorismate Mutase