Influence of culture conditions on growth and protective antigenicity of Clostridium chauvoei.
The effect of culture conditions on growth and immunogenicity of Clostridium chauvoei were examined. The pH control and partial feeding of the carbon source at high concentrations were beneficial for growth. The biomass yield was significatively improved, however the butanol concentration reached toxic levels hampering further growth. For each experimental condition the immunogenicity of cells was tested. No differences were found with cells obtained at different temperatures, but it decreased significatively with the partial supply of the carbon source and pH control.
Cortiñas
T I
TI
Area de Microbiología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Argentina.
Micalizzi
B
B
de Guzman
A M
AM
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000302:isolation & purification; D001431:Bacteriological Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D003013:Clostridium / Q000254:growth & development* / Q000276:immunology* / Q000378:metabolism; D003015:Clostridium Infections / Q000517:prevention & control / Q000662:veterinary; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control; D013696:Temperature
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D005947:Glucose