title: Use of a flavivirus RNA-dependent RNA polymerase assay to investigate the antiviral activity of selected compounds.
abstract: We have developed an assay using flavivirus RNA-dependent RNA polymerase to test the inhibitory activity of potential antiviral agents. The effects of antiviral agents on RNA synthesis were examined in this assay using extracts of Vero cells infected with dengue virus type 2 or Kunjin virus. Several different classes of known polymerase inhibitors were tested. The synthesis of double-stranded replicative form RNA was inhibited in a dose-dependent fashion in the presence of the polyoxometalate HPA-23 [(NH4)18(NaW21Sb9O86)17].14 H2O and several structurally related compounds.
authors.affiliation: Macfarlane Burnet Centre, Fairfield Hospital, Victoria, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Bartholomeusz
authors.forename: E
authors.initials: E
authors.lastname: Tomlinson
authors.forename: P J
authors.initials: PJ
authors.lastname: Wright
authors.forename: C
authors.initials: C
authors.lastname: Birch
authors.forename: S
authors.initials: S
authors.lastname: Locarnini
authors.forename: H
authors.initials: H
authors.lastname: Weigold
authors.forename: S
authors.initials: S
authors.lastname: Marcuccio
authors.forename: G
authors.initials: G
authors.lastname: Holan
mesh_terms: D000818:Animals; D000965:Antimony / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D003715:Dengue / Q000821:virology; D003716:Dengue Virus / Q000187:drug effects / Q000502:physiology; D018349:Encephalitis Viruses, Japanese / Q000187:drug effects; D004671:Encephalitis, Arbovirus / Q000821:virology; D005416:Flavivirus / Q000201:enzymology*; D008826:Microbial Sensitivity Tests; D009942:Organometallic Compounds / Q000494:pharmacology; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis / Q000037:antagonists & inhibitors*; D017973:Tungsten Compounds / Q000494:pharmacology; D014709:Vero Cells / Q000821:virology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D009942:Organometallic Compounds; D017973:Tungsten Compounds; C009613:ammonium tungsten antimonate hydroxide oxide; D000965:Antimony; D012324:RNA-Dependent RNA Polymerase