title: Diaspirin crosslinked hemoglobin (DCLHb): effect of hemodilution during focal cerebral ischemia in rats.
abstract: The efficacy of hemodilution therapy, to ameliorate cerebral ischemia, is limited by an accompanying decrease in oxygen content. We assessed the effect of hemodilution, with diaspirin cross-linked hemoglobin (DCLHb), on cerebral blood flow (CBF) and infarct after middle cerebral artery occlusion (MCAo). Rats (n = 36) were alloted to a control group in which hematocrit (Hct) was not manipulated, or reduced with DCLHb to 30% (30/DCLHb), 16% (16/DCLHb), or 9% (9/DCLHb). After MCAo, the brain area with a CBF of 0-10 ml.100g-1.min-1 was determined. This area was decreased in the 30/DCLHb and 16/DCLHb groups vs the Control group; and was less in the 9/DCLHb group vs the other groups. Different rats (n = 49) were hemodiluted with DCLHb or Albumin (Alb): Control, 30/Alb, 30/DCLHb, 16/DCLHb, or 9/DCLHb. After 3-hr of MCAo and 2-hr of reperfusion, infarct area was determined. Brain infarct was less in the 30/DCLHb and 16/DCLHb groups vs the Control and 30/Alb groups; and was less in the 9/DCLHb group vs the other groups. The results of this study support the hypothesis that hemodilution with DCLHb decreases cerebral ischemia in a dose-dependent manner, and in terms of brain ischemia is a more proficient hemodiluting fluid than albumin.
authors.affiliation: Department of Anesthesiology, Loma Linda University, CA 92354.
authors.forename: D J
authors.initials: DJ
authors.lastname: Cole
authors.forename: R M
authors.initials: RM
authors.lastname: Schell
authors.forename: J C
authors.initials: JC
authors.lastname: Drummond
mesh_terms: D000818:Animals; D001241:Aspirin / Q000031:analogs & derivatives* / Q000494:pharmacology; D001802:Blood Substitutes / Q000494:pharmacology*; D001810:Blood Volume; D002545:Brain Ischemia / Q000097:blood / Q000503:physiopathology / Q000628:therapy*; D002544:Cerebral Infarction / Q000097:blood / Q000503:physiopathology / Q000628:therapy; D002560:Cerebrovascular Circulation; D006400:Hematocrit; D006438:Hemodilution; D006454:Hemoglobins / Q000494:pharmacology*; D006801:Humans; D051381:Rats; D011918:Rats, Inbred SHR; D012709:Serum Albumin / Q000494:pharmacology
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D012709:Serum Albumin; C083478:diaspirin-cross-linked hemoglobin; D001241:Aspirin