[Ultramicro ELISA to the detection of IgM antibodies in Mycobacterium leprae using dry blood samples].
In this work the adequate conditions for the detection of IgM antibodies to PGL-I in eluates from dried blood spots using the UMELISA HANSEN and the SUMA technology are established. A total of 300 blood donors and 58 leprosy patients samples were studied. For both populations, in the previously established conditions, we compared the results of the eluates from dried blood spots with the serum samples, and was obtained a correlation of 0.919 in blood donors, 0.969 in patients, and 0.954 for the total of both populations. It was also obtained a level of agreement of 98% in patients and 96% in blood donors. In the patients population was found a sensitivity of 93% and an specificity of 100% for the eluates of dried blood spots evaluated by the UMELISA HANSEN in the comparison with the serum samples analized by the same assay.
Torrella
A
A
Centro de Inmunoensayo, Ciudad Habana, Cuba.
Solis
R L
RL
Rodriguez
N
N
Medina
Y
Y
Pita
M
M
Perez
I
I
Licourt
N
N
D000907:Antibodies, Bacterial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D015440:Leprosy, Lepromatous / Q000097:blood / Q000175:diagnosis*; D009166:Mycobacterium leprae / Q000276:immunology*; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D007075:Immunoglobulin M
UltramicroELISA para la detección de anticuerpos IgM al Mycobacterium leprae utilizando muestras de sangre seca.