Translation initiation from non-AUG codons in COS1 cells is mRNA species dependent.
Sendai virus P/C mRNA, human erythrocyte membrane protein 4.1 mRNA and PDGF-B chain mRNA were used to test whether translation initiation from non-AUG codons in COS1 cells was mRNA species dependent. Site-directed mutants of the authentic translation start sites of these mRNAs to alternate start codons showed that while P/C mRNA is capable of initiating translation from non-AUG start sites the other two mRNAs are not. Our study shows that translation initiation from non-AUG codons is mRNA species dependent and suggests that higher order structure of an mRNA determines the non-AUG translation start site.
Gupta
K C
KC
Department of Immunology/Microbiology, Rush Medical College, Chicago, IL.
Ono
E
E
Ariztia
E V
EV
Inaba
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M
D000818:Animals; D001483:Base Sequence; D000077214:Becaplermin; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D003062:Codon / Q000378:metabolism*; D003598:Cytoskeletal Proteins; D004910:Erythrocyte Membrane / Q000378:metabolism; D005814:Genes, Viral; D006801:Humans; D007668:Kidney; D008565:Membrane Proteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009479:Neuropeptides; D009690:Nucleic Acid Conformation; D010222:Parainfluenza Virus 1, Human / Q000235:genetics; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017384:Sequence Deletion; D013045:Species Specificity
D003062:Codon; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D009479:Neuropeptides; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D011994:Recombinant Proteins; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1; D000077214:Becaplermin