Porcine luteal cells express monocyte chemoattractant protein-2 (MCP-2): analysis by cDNA cloning and northern analysis.
From an expression library in lambda UniZAP, derived from porcine corpus luteum (CL), a clone lambda MCP9 was detected by hybridization with a porcine MCP-1 specific probe. A pBluescriptSK-derivative pMCP9 was generated from lambda MCP9 by in vivo excision and was shown to contain an open reading frame (ORF) encoding a protein highly homologous to bovine monocyte chemoattractant protein-2 (MCP-2). Comparison of amino acid sequences of known MCPs identified the protein encoded by pMCP9 as porcine MPC-2. The 3' untranslated region of pMCP9 was completed by 3' RACE. Northern analysis using RNA from porcine luteal cells and probes specific for porcine MCP-1 and MCP-2 revealed that porcine luteal cells express both MCPs. According to Southern analysis MCP-2, like MCP-1, is specified by a single copy gene.
Hosang
K
K
Dept. of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany.
Knoke
I
I
Klaudiny
J
J
Wempe
F
F
Wuttke
W
W
Scheit
K H
KH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D054412:Chemokine CCL8; D002630:Chemotactic Factors / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D003338:Corpus Luteum / Q000378:metabolism*; D018076:DNA, Complementary; D005260:Female; D008969:Molecular Sequence Data; D018945:Monocyte Chemoattractant Proteins; D017386:Sequence Homology, Amino Acid; D013552:Swine
D054412:Chemokine CCL8; D002630:Chemotactic Factors; D018076:DNA, Complementary; D018945:Monocyte Chemoattractant Proteins