title: Mannose transporter of Escherichia coli. Backbone assignments and secondary structure of the IIA domain of the IIABMan subunit.
abstract: The mannose transporter of Escherichia coli consists of two transmembrane and one peripheral protein subunit. The complex acts by a mechanism which couples translocation of the substrate with substrate phosphorylation. The peripheral IIABMan is a homodimer. The IIABMan monomer itself contains two domains which are linked by an Ala-Pro-rich hinge and which are both transiently phosphorylated at histidyl residues. The IIA and IIB domains can be separated by limited proteolysis. The IIA domain has a dimer molecular mass of 2 x 14 kDa. Almost complete 1H, 13C, and 15N NMR assignments of the backbone resonances of IIAMan have been achieved using 3D and 4D double-and triple-resonance techniques. Secondary structure elements were derived from NOE data. The IIA domain consists of a central beta-sheet of four parallel and one antiparallel strand (strand order 5 4 3 1 2) with helices on both sides of the sheet. The active-site His-10 is located in a loop at the C-terminus of beta-strand 1. This loop and the loop after strand 3 are at the topological switch point of the sheet.
authors.affiliation: Institut für Organische Chemie und Biochemie, Technische Universität München, Garching, FRG.
authors.forename: S
authors.initials: S
authors.lastname: Seip
authors.forename: J
authors.initials: J
authors.lastname: Balbach
authors.forename: S
authors.initials: S
authors.lastname: Behrens
authors.forename: H
authors.initials: H
authors.lastname: Kessler
authors.forename: K
authors.initials: K
authors.lastname: Flükiger
authors.forename: R
authors.initials: R
authors.lastname: de Meyer
authors.forename: B
authors.initials: B
authors.lastname: Erni
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000378:metabolism*; D037241:Mannose-Binding Lectins; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D017433:Protein Structure, Secondary
chemical_list: D002352:Carrier Proteins; D037241:Mannose-Binding Lectins; D009838:Oligodeoxyribonucleotides; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D008358:Mannose