A corolla- and carpel-abundant, non-specific lipid transfer protein gene is expressed in the epidermis and parenchyma of Gerbera hybrida var. Regina (Compositae).
We are examining the floral organ differentiation in Compositae by isolating and characterizing corolla abundant genes. Differential screening of a cDNA library made from the ray floret corolla of Gerbera hybrida var. Regina revealed an abundant cDNA clone which is expressed in the corolla but not in leaves. This cDNA (gltp1) codes for a polypeptide similar to non-specific lipid transfer proteins of the plants. The gltp1 gene is expressed only in the corolla and carpels and is developmentally regulated during corolla development. The gltp1 mRNA accumulates both in epidermal cell layers and in the mesophyll of the corolla. In the stylar part of the carpel, the gltp1 mRNA can be detected in the epidermal and in parenchymal cells but not in the transmitting tissue. Analogous patterns of gltp1 expression in the corolla and carpel may indicate that similar genetic programmes operates during the development of these two tissues.
Kotilainen
M
M
Institute of Biotechnology, University of Helsinki, Finland.
Helariutta
Y
Y
Elomaa
P
P
Paulin
L
L
Teeri
T H
TH
D000595:Amino Acid Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D002454:Cell Differentiation; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D063245:Plant Development; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification; D018520:Plant Shoots / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution
D002352:Carrier Proteins; D018076:DNA, Complementary; C091032:GLTP1 protein, Gerbera hybrida; D047908:Intracellular Signaling Peptides and Proteins; D010940:Plant Proteins; D012333:RNA, Messenger