title: Dopamine inhibits TRH-induced MAP kinase activation in dispersed rat anterior pituitary cells.
abstract: We recently reported the existence of two separate pathways for thyrotropin-releasing hormone (TRH)-induced mitogen-activated protein (MAP) kinase activation in GH3 pituitary tumor cells. To test the role of MAP kinase in TRH action, we examined the effect of dopamine (DA) on TRH-induced MAP kinase in primary cultures of rat anterior pituitary cells. 1 microM of DA attenuated 1 microM TRH-induced MAP kinase activity and phosphorylation. 100 ng/ml of islet-activating protein (IAP) blocked these inhibitory effects of DA. These results suggest that crosstalk exists between the DA signaling pathway and the TRH-stimulated MAP kinase activating pathway in rat anterior pituitary cells.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohmichi
authors.forename: K
authors.initials: K
authors.lastname: Koike
authors.forename: A
authors.initials: A
authors.lastname: Nohara
authors.forename: Y
authors.initials: Y
authors.lastname: Kanda
authors.forename: T
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Z X
authors.initials: ZX
authors.lastname: Zhang
authors.forename: K
authors.initials: K
authors.lastname: Hirota
authors.forename: A
authors.initials: A
authors.lastname: Miyake
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D002478:Cells, Cultured; D004298:Dopamine / Q000494:pharmacology* / Q000502:physiology; D004789:Enzyme Activation; D037342:Pertussis Toxin; D010766:Phosphorylation; D010903:Pituitary Gland, Anterior / Q000201:enzymology*; D051381:Rats; D015398:Signal Transduction; D013973:Thyrotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D010566:Virulence Factors, Bordetella; D013973:Thyrotropin-Releasing Hormone; D000255:Adenosine Triphosphate; D037342:Pertussis Toxin; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D004298:Dopamine