A pathway for cell wall anchorage of Saccharomyces cerevisiae alpha-agglutinin.
Saccharomyces cerevisiae alpha-agglutinin is a cell wall-anchored adhesion glycoprotein. The previously identified 140-kDa form, which contains a glycosyl-phosphatidylinositol (GPI) anchor (D. Wojciechowicz, C.-F. Lu, J. Kurjan, and P. N. Lipke, Mol. Cell. Biol. 13:2554-2563, 1993), and additional forms of 80, 150, 250 to 300, and > 300 kDa had the properties of intermediates in a transport and cell wall anchorage pathway. N glycosylation and additional modifications resulted in successive increases in size during transport. The 150- and 250- to 300-kDa forms were membrane associated and are likely to be intermediates between the 140-kDa form and a cell surface GPI-anchored form of > 300 kDa. A soluble form of > 300 kDa that lacked the GPI anchor had properties of a periplasmic intermediate between the plasma membrane form and the > 300-kDa cell wall-anchored form. These results constitute experimental support for the hypothesis that GPI anchors act to localize alpha-agglutinin to the plasma membrane and that cell wall anchorage involves release from the GPI anchor to produce a periplasmic intermediate followed by linkage to the cell wall.
Lu
C F
CF
Department of Biological Sciences, Hunter College, City University of New York, New York 10021.
Kurjan
J
J
Lipke
P N
PN
D002473:Cell Wall / Q000502:physiology*; D019286:Endopeptidase K; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D007700:Kinetics; D000072235:Mating Factor; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010452:Peptide Biosynthesis; D010455:Peptides / Q000302:isolation & purification; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D012697:Serine Endopeptidases / Q000378:metabolism
D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D010169:Palmitic Acids; D010455:Peptides; D019308:Palmitic Acid; D007294:Inositol; D000072235:Mating Factor; D008715:Methionine; D010727:Phosphoric Diester Hydrolases; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D012697:Serine Endopeptidases; D019286:Endopeptidase K; D043265:Phosphatidylinositol Diacylglycerol-Lyase