title: Isolation, characterization, and expression of a second beta-tubulin-encoding gene from Colletotrichum gloeosporioides f. sp. aeschynomene.
abstract: Colletotrichum gloeosporioides f. sp. aeschynomene is a fungal plant pathogen of Aeschynomene virginica. A beta-tubulin-encoding gene (TUB2) from this pathogen was cloned and sequenced. The deduced amino acid sequence of TUB2 had a high degree of homology to other fungal beta-tubulins. A portion of TUB2 from a benomyl-resistant C. gloeosporioides f. sp. aeschynomene mutant was also cloned and sequenced. A point mutation resulting in a glutamic acid-to-lysine substitution at amino acid 198 likely confers benomyl resistance. The mutation is relevant for use as a selectable marker in developing a gene transfer system in C. gloeosporioides f. sp. aeschynomene. Northern (RNA) hybridizations with C. gloeosporioides f. sp. aeschynomene TUB2 and another C. gloeosporioides f. sp. aeschynomene beta-tubulin-encoding gene (TUB1) as probes showed differential expression of these genes in different cell types.
authors.affiliation: Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
authors.forename: T L
authors.initials: TL
authors.lastname: Buhr
authors.forename: M B
authors.initials: MB
authors.lastname: Dickman
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001542:Benomyl / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology; D005800:Genes, Fungal / Q000235:genetics*; D003904:Mitosporic Fungi / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D017354:Point Mutation / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014404:Tubulin / Q000235:genetics*
chemical_list: D014404:Tubulin; D001542:Benomyl