title: Mistranslation of human phosphoglycerate kinase in yeast in the presence of paromomycin.
abstract: Missense errors in the translation of mRNAs in Saccharomyces cerevisiae were screened by looking for charge heterogeneity of proteins on two-dimensional gels resulting from the substitution of charged and neutral amino acids. No such mistranslation was detected in wild-type yeast strains grown in the presence of the translational error-inducing antibiotic paromomycin. However, paromomycin-induced mistranslation of a heterologous mRNA, encoding human phosphoglycerate kinase expressed in yeast, was seen. We suggest that the combination of error-prone translation of a heterologous mRNA, and growth in the presence of paromomycin, leads to an accumulation of mistranslated proteins that can be detected by two-dimensional gel electrophoresis.
authors.affiliation: Biological Laboratory, University of Kent, Canterbury, UK.
authors.forename: C M
authors.initials: CM
authors.lastname: Grant
authors.forename: M F
authors.initials: MF
authors.lastname: Tuite
mesh_terms: D019747:Aldose-Ketose Isomerases; D001345:Autoradiography; D003001:Cloning, Molecular / Q000187:drug effects*; D005656:Fungal Proteins / Q000096:biosynthesis; D005800:Genes, Fungal / Q000187:drug effects; D006801:Humans; D008715:Methionine / Q000378:metabolism; D010303:Paromomycin / Q000494:pharmacology*; D010735:Phosphoglycerate Kinase / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013462:Sulfur Radioisotopes
chemical_list: D005656:Fungal Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D013462:Sulfur Radioisotopes; D010303:Paromomycin; D008715:Methionine; D010735:Phosphoglycerate Kinase; D019747:Aldose-Ketose Isomerases; C490353:TRP1 protein, S cerevisiae