title: A rapid and efficient PCR-based method for synthesizing high-molecular-weight multimers of oligonucleotides.
abstract: We have developed a simple PCR based approach for synthesizing high-molecular-weight DNA multimers of short oligonucleotide elements in yields sufficient for preparing DNA affinity columns or for using as probes to detect sequence-specific DNA binding proteins. The technique is rapid, efficient and produces multimers consisting of 30-100 repeating units.
authors.affiliation: Laboratory of Structural Biology and Molecular Medicine, UCLA School of Medicine 90024.
authors.forename: F
authors.initials: F
authors.lastname: Hemat
authors.forename: K
authors.initials: K
authors.lastname: McEntee
mesh_terms: D001483:Base Sequence; D001704:Biopolymers; D002846:Chromatography, Affinity; D004247:DNA / Q000138:chemical synthesis*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001704:Biopolymers; D004247:DNA