[Relationship of histoplasmin intradermal tests and antibodies levels detected by ELISA and immunodiffusion].
A prospective study was carried out in two groups of individuals: a group 1 (n = 40) included workers from a poultry farm, with potential occupational risk of exposure to Histoplasma capsulatum, etiologic agent of histoplasmosis, and a group 2 (n = 16), persons without occupational risk of exposure to the agent. Histoplasmin skin test was performed in both groups, and three sera were obtained from each individual: 1) before skin test was done, 2) 30 days after, and 3) 180 days after it. In both groups the histoplasmin skin test, even when the test was positive, was not a sufficient antigenic booster to provoke an increase in the H. capsulatum antibody levels capable to be detected by the serologic tests used (ELISA and Double Immunodiffusion). These results contribute to improve the interpretation of ELISA test values in the diagnosis of histoplasmosis.
Fernandez-Andreu
C M
CM
Laboratorio de Micología, Instituto de Medicina Tropical Pedro Kourí (IPK), La Habana, Cuba.
Cadre-Raton
A M
AM
Martinez Machin
G
G
Llop Iiernandez
A
A
Suarez Iiernandez
M
M
D000908:Antibodies, Fungal / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006658:Histoplasma / Q000276:immunology*; D006659:Histoplasmin; D006660:Histoplasmosis / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D005779:Immunodiffusion; D007428:Intradermal Tests; D011446:Prospective Studies
D000908:Antibodies, Fungal; D006659:Histoplasmin
Relación entre la prueba intradérmica de histoplasmina y los niveles de anticuerpos detectables por ELISA e inmunodifusión.