title: Rapid characterization of four species of the Saccharomyces sensu stricto complex according to mitochondrial DNA patterns.
abstract: Several strains of the four sibling species of the genus Saccharomyces (S. bayanus, S. cerevisiae, S. paradoxus, and S. pastorianus) were characterized by using a rapid and simple method of restriction analysis of mitochondrial DNA. Patterns obtained with four-cutter endonucleases (such as AluI, DdeI, HinfI, and RsaI) made it possible to differentiate each species. S. cerevisiae and S. paradoxus presented a greater number of large fragments than S. pastorianus and S. bayanus with all the assay enzymes. With AluI and DdeI, species-specific bands clearly permitted differentiation between S. pastorianus and S. bayanus. To test the resolution of this method, wild Saccharomyces strains were analyzed. The correct assignment of these strains to a known taxon by this rapid method was confirmed by means of electrophoretic karyotyping.
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias Biológicas, Universitat de València, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Guillamón
authors.forename: E
authors.initials: E
authors.lastname: Barrio
authors.forename: T
authors.initials: T
authors.lastname: Huerta
authors.forename: A
authors.initials: A
authors.lastname: Querol
mesh_terms: D004271:DNA, Fungal / Q000032:analysis*; D004272:DNA, Mitochondrial / Q000032:analysis*; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial