title: Effects of retinol on glycoprotein synthesis by Sertoli cells in culture: dolichyl phosphomannose synthase activation.
abstract: Sertoli cells were isolated from Wistar rats aged 19 days and cultured for 48 h. The addition of retinol (10 microM) to the culture medium significantly stimulated the incorporation of [2-3H]mannose into lipid-linked oligosaccharide and into cellular and secreted glycoproteins. Incorporation of [U-14C] leucine into proteins and of [5, 6-3H] uridine into RNA was unaffected by retinol treatment. Incubation of microsomal fractions of retinol-treated cells showed an increase in mannose incorporation into dolichyl phosphomannose, into dolichyl pyrophosphoryl oligosaccharide and into proteins. Chromatographic analysis of the fraction soluble in chloroform/methanol (2:1 v/v) did not show the presence of retinyl phosphomannose either in control or in retinol-treated cells. When the formation of dolichyl phosphomannose was studied in microsomes isolated from control cells and from cells treated with 10 microM retinol for 48 h in the presence of exogenous dolichyl phosphate, the results showed that the retinol effect was due to stimulation of dolichyl phosphomannose synthase.
authors.affiliation: Departamento de Bioquimica, IB, UFRGS, Porto Alegre, RS, Brasil.
authors.forename: F C
authors.initials: FC
authors.lastname: Guma
authors.forename: E A
authors.initials: EA
authors.lastname: Bernard
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004789:Enzyme Activation; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation; D008297:Male; D008364:Mannosyltransferases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D014801:Vitamin A / Q000494:pharmacology*
chemical_list: D006023:Glycoproteins; D014801:Vitamin A; D008364:Mannosyltransferases; C022755:dolichyl-phosphate beta-D-mannosyltransferase