Glutathione S-transferase expression in renal cell carcinoma: a new marker of differentiation.
Expression of two GST isoenzymes (alpha and pi) was assessed immunohistochemically in 46 cases of renal cell carcinoma (RCC) for which clinical follow-up was available. In the normal kidney GST alpha stained most intensely in the proximal convoluted tubules and GST pi in distal tubules/collecting ducts. All 46 tumors studied stained positively for GST alpha with most demonstrating strong cytoplasmic and nuclear reactivity. GST pi immunostaining was positive in 35/46 cases (76%). Five-year survival for patients with GST pi positive tumors was 88% versus 50% for those with GST pi negative tumors (P < 0.008). Loss of GST mRNA has been reported in RCC and it has been suggested that this may represent a dedifferentiation program in RCC. These data support this hypothesis and further indicate a potential value for GST pi as a prognostic indicator in RCC.
Grignon
D J
DJ
Department of Pathology, Victoria Hospital, London Regional Cancer Center, Canada.
Abdel-Malak
M
M
Mertens
W C
WC
Sakr
W A
WA
Shepherd
R R
RR
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000201:enzymology* / Q000473:pathology; D005260:Female; D005982:Glutathione Transferase / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis*; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000473:pathology; D007685:Kidney Tubules, Collecting / Q000201:enzymology; D007686:Kidney Tubules, Distal / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000201:enzymology; D008297:Male; D008875:Middle Aged
D014408:Biomarkers, Tumor; D007527:Isoenzymes; D005982:Glutathione Transferase