Lipid peroxidation and homocysteine induced toxicity.
Homocysteine induced toxicity has been examined in cultures of human umbilical vein endothelial cells. The toxic effects of the amino acid alone and the amino acid plus Cu2+ could be prevented by catalase and decreased by desferal, when either was present in the culture medium. When desferal was allowed to accumulate intracellularly, no significant protection from homocysteine induced toxicity was observed. Even though lipid peroxidation accompanied the toxicity induced by homocysteine and homocysteine plus Cu2+, inhibition of lipid peroxidation in either case had no effect on cell viability. The significance of these results is discussed.
Jones
B G
BG
Department of Biochemistry, University of Wales College of Cardiff, UK.
Rose
F A
FA
Tudball
N
N
D002374:Catalase / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D003676:Deferoxamine / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000378:metabolism*; D006710:Homocysteine / Q000633:toxicity*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D017392:Thiobarbituric Acid Reactive Substances; D014471:Umbilical Veins
D017392:Thiobarbituric Acid Reactive Substances; D006710:Homocysteine; D006861:Hydrogen Peroxide; D002374:Catalase; D003676:Deferoxamine