Drosophila P element transposase induces male recombination additively and without a requirement for P element excision or insertion.
P element dysgenesis-associated male recombination in Drosophila was examined with a selective system focused upon a section of the third chromosome divided into eight recombination segments. Tests compared crossing over in the presence of none, one and two doses of P(delta 2-3)(99B), a non-mobile transposase source, in the absence of a mobilizable P element target in the genome. In the presence of the P transposase source, and without a P element target, significant male recombination occurred in genomic regions physically separated from the P(delta 2-3) site. Using two doses of P(delta 2-3) without a P element target, the male recombination rate doubled, and 90% of the crossovers occurred in the pericentric region. The distribution of recombination events, in the absence of P element targets approximates that seen in studies of radiation induced mitotic crossing over and the metaphase chromosome map. Another experiment examined the effects of one dose of P(delta 2-3) on a genome with a single P element target, P(lArB)(87C9), in the third recombination segment. Crossovers increased 58-fold in the immediate region of the P element target.
McCarron
M
M
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-2131.
Duttaroy
A
A
Doughty
G
G
Chovnick
A
A
D000818:Animals; D003434:Crossing Over, Genetic; D004251:DNA Transposable Elements; D004330:Drosophila / Q000201:enzymology / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D015321:Gene Rearrangement; D017344:Genes, Insect; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D005819:Genetic Markers; D008297:Male; D009713:Nucleotidyltransferases / Q000235:genetics*; D011995:Recombination, Genetic; D019895:Transposases
D004251:DNA Transposable Elements; D005819:Genetic Markers; D009713:Nucleotidyltransferases; D019895:Transposases