Analysis by two-dimensional gel electrophoresis of ram epididymal secreted proteins.
We used two-dimensional gel electrophoresis to analyse the 35S-labelled proteins secreted in vitro by the epithelium of the epididymis. Polypeptides with molecular weight ranging from 10 to > 200 kDa and isoelectric points from < 4 to 9 were observed. These compounds showed high degree of polymorphism. Some were secreted in distinct zones while others were present in the caput and the cauda epididymis.
Druart
X
X
URA 1291 INRA-CNRS, Institut National de la Recherche Agronomique, Monnaie, France.
Gatti
J L
JL
Dacheux
F
F
Dacheux
J L
JL
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D004822:Epididymis / Q000378:metabolism*; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012756:Sheep
D011506:Proteins