The effect of mouse mammary tumor virus receptor activation on mammary epithelial cell sensitivity toward prolactin.
Mammary epithelial cells infected with the mouse mammary tumor virus (MMTV) require less than one-half the concentration of prolactin to elicit alpha-lactalbumin production than uninfected tissue (EC50 = 89 +/- 10 ng/ml vs. 206 ng/ml, respectively). Furthermore, stimulating antibodies to the cellular receptor for MMTV halved the prolactin requirement of MMTV-tissue, while MMTV antibodies that sequestered secreted MMTV increased the prolactin requirement in MMTV+ tissue. These data suggest that the effect of MMTV on mammary epithelial sensitivity toward prolactin is being mediated by its interaction with a cell surface receptor.
Bolander
F F
FF
Department of Biological Sciences, University of South Carolina, Columbia 29208.
D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000821:virology; D005260:Female; D008321:Mammary Glands, Animal / Q000166:cytology / Q000821:virology*; D008324:Mammary Tumor Virus, Mouse / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D011388:Prolactin / Q000502:physiology*; D011991:Receptors, Virus / Q000502:physiology*; D012756:Sheep; D013552:Swine
D011991:Receptors, Virus; D011388:Prolactin