title: Changes in carnitine palmitoyltransferase-I mRNA abundance produced by hyperthyroidism and hypothyroidism parallel changes in activity.
abstract: To study the regulation of carnitine palmitoyltransferase-I by thyroid hormone, a cDNA was obtained by PCR amplification of DNA obtained by reverse transcription of rat liver RNA. CPT-I mRNA abundance was measured in livers of hyperthyroid, euthyroid and hypothyroid rats. In hypothyroid rats, the CPT-I mRNA levels decreased 40-fold relative to that of the hyperthyroid animals. These changes paralleled alterations in enzyme activity. These data suggest that CPT-I is regulated at the transcriptional level by thyroid hormone.
authors.affiliation: Department of Pharmacology, University of Tennessee, Memphis 38163.
authors.forename: R L
authors.initials: RL
authors.lastname: Mynatt
authors.forename: E A
authors.initials: EA
authors.lastname: Park
authors.forename: F E
authors.initials: FE
authors.lastname: Thorngate
authors.forename: H K
authors.initials: HK
authors.lastname: Das
authors.forename: G A
authors.initials: GA
authors.lastname: Cook
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002334:Carnitine O-Palmitoyltransferase / Q000096:biosynthesis / Q000378:metabolism*; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D006980:Hyperthyroidism / Q000201:enzymology*; D007037:Hypothyroidism / Q000201:enzymology*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011441:Propylthiouracil; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013379:Substrate Specificity; D013963:Thyroid Hormones / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D013963:Thyroid Hormones; D011441:Propylthiouracil; D002334:Carnitine O-Palmitoyltransferase