Characterization of urokinase-type plasminogen activator of rat decidual tissue.
Urokinase-type plasminogen activator (uPA) from artificially induced decidual tissue of rat has been purified to homogeneity employing chromatographic techniques and the final preparation has a specific activity of 12,084 I.U./mg. The purified preparation resolves into a single band following SDS-PAGE and has an apparent molecular weight of 45 kDa. HPLC of the purified fraction also yields a single peak at 45 kDa. Decidual uPA is immunogenic in rabbit and a monospecific antiserum raised against it does not cross react with human melanoma tPA or rat Yoshida sarcoma tPA but elicits a precipitin reaction with human uPA and extracts of rat placenta and kidney. The enzyme has a pH optimum of 7.5, a kM of 1.0 microM, is heat stable upto ten minutes at 42 degrees C and inhibited by anti-uPA IgG.
Pawse
A R
AR
Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Bombay.
Tarachand
U
U
D000818:Animals; D003429:Cross Reactions; D003656:Decidua / Q000201:enzymology*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007668:Kidney / Q000201:enzymology; D008545:Melanoma / Q000201:enzymology; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000276:immunology; D009928:Organ Specificity; D011257:Pregnancy Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D011489:Protein Denaturation; D011555:Pseudopregnancy; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012517:Sarcoma, Yoshida / Q000201:enzymology; D013045:Species Specificity; D010959:Tissue Plasminogen Activator / Q000276:immunology; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D007106:Immune Sera; D009363:Neoplasm Proteins; D011257:Pregnancy Proteins; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator