Changes in carnitine palmitoyltransferase-I mRNA abundance produced by hyperthyroidism and hypothyroidism parallel changes in activity.
To study the regulation of carnitine palmitoyltransferase-I by thyroid hormone, a cDNA was obtained by PCR amplification of DNA obtained by reverse transcription of rat liver RNA. CPT-I mRNA abundance was measured in livers of hyperthyroid, euthyroid and hypothyroid rats. In hypothyroid rats, the CPT-I mRNA levels decreased 40-fold relative to that of the hyperthyroid animals. These changes paralleled alterations in enzyme activity. These data suggest that CPT-I is regulated at the transcriptional level by thyroid hormone.
Mynatt
R L
RL
Department of Pharmacology, University of Tennessee, Memphis 38163.
Park
E A
EA
Thorngate
F E
FE
Das
H K
HK
Cook
G A
GA
D000818:Animals; D015152:Blotting, Northern; D002334:Carnitine O-Palmitoyltransferase / Q000096:biosynthesis / Q000378:metabolism*; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D006980:Hyperthyroidism / Q000201:enzymology*; D007037:Hypothyroidism / Q000201:enzymology*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011441:Propylthiouracil; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013379:Substrate Specificity; D013963:Thyroid Hormones / Q000502:physiology; D014158:Transcription, Genetic
D012333:RNA, Messenger; D013963:Thyroid Hormones; D011441:Propylthiouracil; D002334:Carnitine O-Palmitoyltransferase