Characterization of an antibiotic produced by Alteromonas luteoviolacea Gauthier 1982, 85 isolated from Kinko Bay, Japan.
An antibiotic produced by Alteromonas luteoviolacea strain 9K-V10 was recovered after cold acetone precipitation of culture supernatant fluids or lysates that had been frozen and thawed. The precipitate obtained from cell-free lysates was fractioned by DEAE ion-exchange chromatography. Further purification by gel-filtration chromatography yielded a single peak of antibiotic activity that corresponded to a protein peak with a molecular mass of approximately 100 kDa. After non-denaturing polyacrylamide gel electrophoresis, antibiotic activity co-migrated with a protein band. The isoelectric point of the antibiotic was estimated to be 7.7. Treatment of the concentrated active fraction with proteinase K or heating at 70 degrees C for 10 min resulted in total loss of antibiotic activity. These results show that the antibiotic produced by Alt. luteoviolacea 9K-V10 is of a proteinaceous nature.
McCarthy
S A
SA
Department of Botany and Microbiology, Arizona State University, Tempe.
Johnson
R M
RM
Kakimoto
D
D
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000494:pharmacology; D005618:Fresh Water; D006088:Gram-Negative Aerobic Bacteria / Q000302:isolation & purification* / Q000378:metabolism*; D006358:Hot Temperature; D007525:Isoelectric Focusing; D007564:Japan; D012623:Seawater; D013211:Staphylococcus aureus / Q000187:drug effects; D014871:Water Microbiology
D000900:Anti-Bacterial Agents; D004798:Enzymes