title: Rat long-term bone marrow culture as a model for transient production of multinucleated giant cells into suspension culture fraction and for steady mineralization process of adherent stromal cells.
abstract: Under in vitro conditions rat bone marrow stromal cells induced into osteogenic differentiation by beta-glycerophosphate, exogenous ascorbic acid and dexamethasone are able to produce a mineralized matrix. Here we describe adult rat long-term bone marrow cultures (LTBMC), deprived of these substances. Mineralization process in stromal adherent cells occurred after 1 month of incubation and was proved by means of X-ray electron microprobe. The high content of sulfur in the extracellular matrix surrounding the mineralized nodules suggests non-distrophic pathway of Ca/P deposition. During the first 2 weeks of incubation the extensive production of multinucleated tartrate-resistant acid phosphatase (TRAP) positive and TRAP negative giant cells occurred into the suspension fraction of the cultures. Rat LTBMC may serve as a model for the investigation of differentiation of osteoblastic progenitors and their interactions with multinucleated TRAP positive cells.
authors.affiliation: Laboratoire de Recherche sur les Tissus Calcifiés et les Biomatériaux, Faculté de Chirurgie Dentaire, Nantes, France.
authors.forename: O I
authors.initials: OI
authors.lastname: Gan
authors.forename: A
authors.initials: A
authors.lastname: Soueidan
authors.forename: A
authors.initials: A
authors.lastname: Castagné
authors.forename: G
authors.initials: G
authors.lastname: Daculsi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002458:Cell Fractionation; D002478:Cells, Cultured; D015726:Giant Cells / Q000166:cytology*; D008903:Minerals / Q000378:metabolism*; D008954:Models, Biological; D051381:Rats; D011917:Rats, Inbred Lew; D017154:Stromal Cells / Q000378:metabolism* / Q000502:physiology
chemical_list: D008903:Minerals