[The use of the induced test of nitroblue tetrazolium reduction for the diagnosis of fungal bronchial asthma].
The NBT reduction test was used to assess the neutrophilic activities in 49 patients with bronchial asthma. 12 healthy donors were examined for control. The NBT test values were lower in health than in asthma: 8.8 +/- +0.97 vs. 12.8 +/- 0.7%, respectively, p < 0.05. Addition of a specific fungal allergen resulted in a twofold increase of the count of formasan-positive cells. A lower, but still a significant increase of the count of active granulocytes was induced in the asthmatics by home dust allergen (17.7 +/- 0.91%). The results of induced NBT test and of the reaction of antigen-specific injury of the granulocytes were in excellent correlation (r = 0.65, p < 0.05). There was no correlation of this test data with those of indirect Shelley's test or skin tests. This fact indicates the contribution of both the immediate and delayed mechanisms to the pathogenesis of fungal bronchial asthma.
Bashir
K
K
Dotsenko
E A
EA
Novikov
D K
DK
D000485:Allergens; D000528:Alternaria / Q000276:immunology; D001231:Aspergillus flavus / Q000276:immunology; D001249:Asthma / Q000175:diagnosis*; D015550:Cell Degranulation; D004391:Dust; D005069:Evaluation Studies as Topic; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D008172:Lung Diseases, Fungal / Q000175:diagnosis*; D008407:Mast Cells / Q000502:physiology; D009580:Nitroblue Tetrazolium; D012233:Rhizopus / Q000276:immunology; D012882:Skin Tests
D000485:Allergens; D004391:Dust; D009580:Nitroblue Tetrazolium
Primenenie indutsirovannogo testa vosstanovleniia nitrosinego tetrazoliia dlia diagnostiki gribkovoĭ bronkhial'noĭ astmy.