title: Subsets of HLA-DR1 molecules defined by SEB and TSST-1 binding.
abstract: Superantigens bind to major histocompatibility complex class II molecules on antigen-presenting cells and stimulate T cells. Staphylococcus aureus enterotoxin B (SEB) and toxic shock syndrome toxin-1 (TSST-1) bind to the same region of human lymphocyte antigen (HLA)-DR1 but do not compete with each other, which indicates that they bind to different subsets of DR1 molecules. Here, a mutation in the peptide-binding groove disrupted the SEB and TSST-1 binding sites, which suggests that peptides can influence the interaction with bacterial toxins. In support of this, the expression of the DR1 molecule in various cell types differentially affected the binding of these toxins.
authors.affiliation: Laboratoire d'Immunologie, Institut de Recherches Cliniques de Montréal, Québec, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Thibodeau
authors.forename: I
authors.initials: I
authors.lastname: Cloutier
authors.forename: P M
authors.initials: PM
authors.lastname: Lavoie
authors.forename: N
authors.initials: N
authors.lastname: Labrecque
authors.forename: W
authors.initials: W
authors.lastname: Mourad
authors.forename: T
authors.initials: T
authors.lastname: Jardetzky
authors.forename: R P
authors.initials: RP
authors.lastname: Sékaly
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D001427:Bacterial Toxins; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D004768:Enterotoxins / Q000737:chemistry / Q000378:metabolism*; D015798:HLA-DR1 Antigen / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006825:Hybridomas; D051379:Mice; D009154:Mutation; D017433:Protein Structure, Secondary; D013211:Staphylococcus aureus; D018089:Superantigens / Q000737:chemistry / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D015798:HLA-DR1 Antigen; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal