title: Granule secretion markers on fluid-phase platelets in whole blood perfused through capillary tubing.
abstract: The effect of material composition and shear rate on fluid-phase platelet activation was investigated using a capillary perfusion model. Citrated whole blood was perfused along the lumens of tubes constructed from silicone, PVC, Pellethane, W124 (an experimental polyetherurethane), and glass. Platelet activation was determined by measuring the increase in alpha-granule membrane protein P-selectin (GMP-140, CD62) and the lysosomal granule membrane protein GP-53 (CD63) on fluid-phase platelets by flow cytometry. All tubes caused an increase over the negative control in the number of P-selectin and GP-53 molecules detectable on the surface of these platelets. The activation response of platelets to changes in shear rate was also investigated. It was found that lysosomal release paralleled alpha-granule release in glass, but not in Pellethane, over a range of wall shear rates (100-1,000 s-1).
authors.affiliation: Department of Clinical Engineering, University of Liverpool.
authors.forename: N P
authors.initials: NP
authors.lastname: Rhodes
authors.forename: M
authors.initials: M
authors.lastname: Zuzel
authors.forename: D F
authors.initials: DF
authors.lastname: Williams
authors.forename: M R
authors.initials: MR
authors.lastname: Derrick
mesh_terms: D015415:Biomarkers; D001792:Blood Platelets / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D066298:In Vitro Techniques; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008954:Models, Biological; D010477:Perfusion; D015539:Platelet Activation / Q000502:physiology; D012212:Rheology
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D016650:Fluorescein-5-isothiocyanate