[Noncovalent immobilization of catalase on antibodies adsorbed on carbon fabric].
Catalase was immobilized on an immunosorbent prepared by anticatalase adsorption on an activated carbon fabric (ACF), and its kinetic parameters were determined. The immobilized catalase activity depended on the binding capacity of anticatalase. Under the optimum conditions (6 micrograms/mg anticatalase, 5.24 nM catalase) the immobilized catalase activity was 1.5-ford higher as compared to soluble catalase. Antibodies stabilized soluble catalase, but decreased its thermostability on immobilization of immunocomplexes on ACF. Noncovalent immobilization of catalase on adsorbed antibodies opens up the way to the use of this approach for immobilization of other oligomeric enzymes.
Litvinchuk
A V
AV
Morozova
A A
AA
Savenkova
M I
MI
Metelitsa
D I
DI
D000327:Adsorption; D000818:Animals; D000906:Antibodies; D001666:Binding Sites, Antibody; D002244:Carbon; D002374:Catalase / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000276:immunology / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics
D000906:Antibodies; D004800:Enzymes, Immobilized; D002244:Carbon; D002374:Catalase
Nekovalentnaia immobilizatsiia katalazy na antitelakh sorbirovannykh ugol'noĭ tkan'iu.