The high potential iron-sulfur protein (HiPIP) from Rhodoferax fermentans is competent in photosynthetic electron transfer.
The functional role of the High Potential Iron-sulfur Protein (HiPIP) from the photosynthetic bacterium Rhodoferax fermentans was investigated. We demonstrated that the HiPIP increased the rate of light-induced oxygen reduction mediated by the photosynthetic reaction center (RC); this stimulation reached half-saturation at [HiPIP]/[RC] ca. 15. The capability of the HiPIP in delivering electrons to the reaction center of Rhodoferax fermentans was demonstrated through kinetic spectrophotometry of cytochrome c-556 oxidation in the presence or in the absence of HiPIP. It is concluded that the HiPIP is competent in the photosynthetic electron transfer chain of Rhodoferax fermentans.
Hochkoeppler
A
A
Department of Biology, University of Bologna, Italy.
Ciurli
S
S
Venturoli
G
G
Zannoni
D
D
D001419:Bacteria / Q000378:metabolism* / Q000528:radiation effects; D001426:Bacterial Proteins; D004579:Electron Transport; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D008027:Light; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins
D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; C007943:high potential iron-sulfur protein; D010100:Oxygen