title: [Interaction of catalytically active antibodies with oligoribonucleotides].
abstract: The interaction of antibodies from blood sera of patients with autoimmune pathology, systemic lupus erythematosus with oligoribonucleotides was studied. The RNA-hydrolyzing activity was shown to be an intrinsic property of autoantibodies. Enzymic activity of antibodies in hydrolysis of poly(U) was estimated at 20-40% of that of RNase A. In contrast to known eukaryotic RNases, the autoantibodies possess a specific RNA-hydrolyzing activity for oligo r(A). The RNA-nicking activity of antibodies in hydrolysis of oligoadenylates was more higher than with hydrolysis of oligo d(A). Optimal conditions of r(pA)13 hydrolysis were selected, including the optimal of pH = 8.7.
authors.forename: V N
authors.initials: VN
authors.lastname: Buneva
authors.forename: O A
authors.initials: OA
authors.lastname: Andrievskaia
authors.forename: I V
authors.initials: IV
authors.lastname: Romannikova
authors.forename: G V
authors.initials: GV
authors.lastname: Gololobov
authors.forename: R P
authors.initials: RP
authors.lastname: Iadav
authors.forename: V I
authors.initials: VI
authors.lastname: Iamkovoĭ
authors.forename: G A
authors.initials: GA
authors.lastname: Nevinskiĭ
mesh_terms: D017151:Antibodies, Catalytic / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009843:Oligoribonucleotides / Q000276:immunology* / Q000378:metabolism
chemical_list: D017151:Antibodies, Catalytic; D001323:Autoantibodies; D009843:Oligoribonucleotides
vernacular_title: Vzaimodeĭstvie kataliticheski aktivnykh antitel s oligoribonukleotidami.