Glycotripeptides are released by yeast but not by mammalian microsomes.
Glycotripeptides generated in vivo in the endoplasmic reticulum (ER) have been used as markers to assess the rate of vesicular bulk flow from the ER via the Golgi apparatus to the plasma membrane in mammalian cells. The applicability of such glycotripeptides as markers for bulk flow along this pathway has been questioned by a report on non-vesicular release of glycotripeptides from yeast semi-intact spheroplasts. We have therefore investigated direct release of glycotripeptides from yeast and from mammalian microsomes and report here that such release is specific to the yeast system and cannot be detected in mammalian microsomes.
van Leyen
K
K
Institut für Biochemie I, Heidelberg, Germany.
Widemann
M
M
Wieland
F
F
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D066298:In Vitro Techniques; D008322:Mammals; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013045:Species Specificity
D015415:Biomarkers; D006020:Glycopeptides; D016244:Guanosine 5'-O-(3-Thiotriphosphate)