Charge-remote fragmentation of peptides derivatized with 4-aminonaphthalenesulphonic acid.
A series of small peptides has been studied by negative-ion fast-atom bombardment mass spectrometry with collision-induced dissociation. It has been found that by derivatizing peptides with 4-aminonaphthalenesulphonic acid in a peptide linkage at the C-terminus, negative-ion formation can be enhanced and fragmentation in collision-induced dissociation reactions controlled. The peptide-naphthalenesulphonates show charge-remote fragmentations and the resultant spectra give sequence information.
Lindh
I
I
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Griffiths
W J
WJ
Bergman
T
T
Sjövall
J
J
D000595:Amino Acid Sequence; D003172:Complement C1 / Q000032:analysis; D004563:Electrochemistry; D004743:Enkephalin, Leucine / Q000032:analysis; D008969:Molecular Sequence Data; D009282:Naphthalenesulfonates / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D003172:Complement C1; D009282:Naphthalenesulfonates; D010446:Peptide Fragments; D010455:Peptides; D004743:Enkephalin, Leucine; C023356:naphthionic acid