Adhesion of coagulase-negative staphylococci and adsorption of plasma proteins to heparinized polymer surfaces.
Protease treatment of cells of coagulase-negative staphylococci reduced the adhesion of bacteria to heparinized polyethylene preadsorbed with serum. Fibronectin (Fn), fibrinogen (Fg), vitronectin, complement factor C3c, plasminogen, laminin and to a low extent albumin were detected on tridodecylmethylammonium chloride (TDMAC)-heparinized polyvinyl chloride (PVC) catheters extirpated from the circulation of patients. Using a perfusion model we show that during the first hours of perfusion with human plasma, Fn and Vn dominate, whereas after 22 h of perfusion Fg is the dominant protein. Field emission scanning electron microscopy and atomic force microscopy studies on TDMAC-heparinized catheters as well as on end-point attached heparinized PVC catheters indicate that quantitatively more Fg than Fn is exposed on these surfaces after prolonged exposure (> 22 h) to human plasma.
Yu
J
J
Department of Medical Microbiology, University of Lund, Sweden.
Montelius
M N
MN
Paulsson
M
M
Gouda
I
I
Larm
O
O
Montelius
L
L
Ljungh
A
A
D000327:Adsorption; D001422:Bacterial Adhesion / Q000187:drug effects*; D001704:Biopolymers; D001798:Blood Proteins / Q000378:metabolism*; D002408:Catheters, Indwelling; D003030:Coagulase / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D011143:Polyvinyl Chloride; D013499:Surface Properties
D001704:Biopolymers; D001798:Blood Proteins; D003030:Coagulase; D011143:Polyvinyl Chloride; D006493:Heparin