title: Rapid detection of rotavirus in faeces using a dipstick system with monoclonal antibodies and colloidal gold as marker.
abstract: Rotavirus (RV) is known to be the most common cause of severe diarrhoea in infants and young children, each year leading to an estimated 800,000-900,000 deaths. RV also infects bovines and other species, with high morbidity and mortality. A rapid and simple 'naked-eye' dipstick system was developed to detect human RV in faeces, using nitrocellulose as solid phase, two monoclonal antibodies, and colloidal gold as marker. The system detects 10(4) viral particles (1-2 ng)/g of faeces. For human RV the specificity and sensitivity were 100% when compared with a commercial latex system, and 99% and 98%, respectively, when correlated with traditional RNA-PAGE, and 100% and 98% compared to an ELISA system.
authors.affiliation: Division of Immunotechnology and Diagnostics, Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.
authors.forename: D
authors.initials: D
authors.lastname: Fernández
authors.forename: I
authors.initials: I
authors.lastname: Valle
authors.forename: R
authors.initials: R
authors.lastname: Llamos
authors.forename: M
authors.initials: M
authors.lastname: Guerra
authors.forename: L
authors.initials: L
authors.lastname: Sorell
authors.forename: J
authors.initials: J
authors.lastname: Gavilondo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology*; D005759:Gastroenteritis / Q000175:diagnosis / Q000821:virology; D017979:Gold Colloid; D006801:Humans; D007223:Infant; D051379:Mice; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000821:virology; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D017979:Gold Colloid