Acute effect of some hormones on the metabolism of polyamines in rat liver in vivo as measured by their biliary excretion.
Groups of 6-11 adult male rats were anesthetized with pentobarbital and thin polyethylene tubings were inserted into the common bile duct and femoral vein. The bile was collected for 1 or 2 h periods within a total of 6-8 h and the excretion of putrescine (PUT), spermidine (SPD) and histamine (HA) was estimated with the aid of high performance liquid chromatography and expressed as nmol per time. In each experiment the first time interval was used as control one and then various hormones (insulin, glucagon, cholecystokinin and dexamethasone) and glucose were infused into a femoral vein. Significant increase of PUT and HA excretion was found after glucose infusion which was prevented by simultaneous infusion of insulin. Moreover, the infusion of cholecystokinin resulted in a similar significant increase of PUT, SPD and HA, while dexamethasone and glucagon were without any considerable effect. Finally, a significant increase in PUT and SPD excretion was observed after severe hypoglycemia due to the infusion of insulin. It is concluded that such approach may be used as a novel method for a study of polyamine metabolism in the liver in vivo.
Langer
P
P
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava.
Matsuzaki
S
S
D000818:Animals; D001647:Bile Acids and Salts / Q000737:chemistry* / Q000378:metabolism; D002766:Cholecystokinin / Q000008:administration & dosage / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology; D005934:Glucagon / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology; D006632:Histamine / Q000032:analysis* / Q000378:metabolism; D007262:Infusions, Intravenous; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000032:analysis* / Q000378:metabolism; D013997:Time Factors
D001647:Bile Acids and Salts; D007328:Insulin; D011073:Polyamines; D003907:Dexamethasone; D006632:Histamine; D005934:Glucagon; D002766:Cholecystokinin; D005947:Glucose; D013095:Spermidine; D011700:Putrescine