A simplified method for manufacturing glass-insulated metal microelectrodes.
A simplified method to manufacture durable, glass-insulated, tungsten microelectrodes with sufficient control of the final electrode impedance is described. This method requires only two instruments, an electrolytic etcher for wires and pipette puller, for manufacturing these electrodes. The manufacture of these electrodes involves 3 steps: (1) etching tungsten wire to sharpen the tip, (2) insulating the electrode by pulling a glass pipette over the sharpened tungsten wire and (3) assessing and adjusting the tip exposure and impedance of the electrode to meet recording requirements. Control over the electrode impedance is easily accomplished by varying the distance between the uppermost portion of the heating coil and the sharpened wire tip before a glass pipette is pulled over the wire tip. This distance determines the area of tip exposure and also the location where the glass insulation ends and the exposed electrode tip begins. A performance test of these electrodes in a chronically prepared monkey showed that they were strong enough to repeatedly penetrate thickened dura mater without significant changes in impedance and to isolate cortical neuronal activity after these multiple penetrations. Furthermore, the strength of these microelectrodes eliminated the need to remove reactive granular tissue from the dura overlying the recording site.
Sugiyama
K
K
Department of Anesthesiology, University of Washington, Seattle 98195.
Dong
W K
WK
Chudler
E H
EH
D000818:Animals; D004388:Dura Mater; D017097:Electric Impedance; D004569:Electroencephalography / Q000295:instrumentation*; D004867:Equipment Design; D005898:Glass; D000882:Haplorhini; D008722:Methods; D008839:Microelectrodes; D014414:Tungsten
D014414:Tungsten