Protective effect of ambroxol against heat- and hydrogen peroxide-induced damage to lung lipids in mice.
We wanted to determine whether ambroxol, a drug which stimulates the release of surfactant by type II pneumocytes, can protect lung lipids from peroxidative damage in mice. Animals were injected intraperitoneally with ambroxol, 0.169 mmol.kg-1, or 1 ml buffer once a day for three consecutive days. Lipid peroxidation was then induced in lung homogenates either by means of heat, 50 degrees C, or H2O2, 10 mmol.l-1. The lung homogenates from ambroxol-treated animals revealed decreased lipid peroxidation in response to both stimuli. The heat- and H2O2-induced generation of conjugated dienes (a first lipid peroxidation product) in ambroxol-treated lung homogenates was 3.7 and 3.1 fold lower than in the lungs from buffer-injected mice. Ambroxol, as an inhibitor of heat- and H2O2-induced lipid peroxidation, was equipotent to and stronger than the two antioxidants, N-acetylcysteine and methionine, respectively. Ambroxol was not able to protect heart and liver lipids. These results suggest that ambroxol can sufficiently enhance the antioxidant defence in lung tissue and can act as a lung lipid antioxidant.
Nowak
D
D
Dept of Pneumology and Allergology, Medical University of Lodz, Poland.
Antczak
A
A
Pietras
T
T
Bialasiewicz
P
P
Krol
M
M
D000111:Acetylcysteine / Q000494:pharmacology; D000551:Ambroxol / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D006358:Hot Temperature / Q000009:adverse effects; D006861:Hydrogen Peroxide / Q000009:adverse effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011663:Pulmonary Surfactants / Q000187:drug effects*
D000975:Antioxidants; D011663:Pulmonary Surfactants; D000551:Ambroxol; D008715:Methionine; D006861:Hydrogen Peroxide; D000111:Acetylcysteine