Immunohistochemical distributions of cathepsin B and basement membrane antigens in human lung adenocarcinoma: association with invasion and metastasis.
The distributions of cathepsin B (CB) a lysosomal cysteine proteinase, type IV collagen (CIV) and laminin (LM), which are main components of basement membranes (BMs) were studied in a series of 64 human lung adenocarcinomas using an immunohistochemical technique. Over-expression of CB (> 80% positive cells) was significantly associated with the grade of tumour differentiation (p < 0.01), with lymph node metastasis (p < 0.01) and with BM degradation (p < 0.01) detected by the staining pattern of CIV and LM. It was significantly associated with a prognostic disadvantage (p < 0.01). The immunohistochemical staining pattern of CB has a close relationship with degradation of BM, and may be used as a marker for tumour metastasis and prognosis in lung adenocarcinoma.
Sukoh
N
N
First Department of Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
Abe
S
S
Nakajima
I
I
Ogura
S
S
Isobe
H
H
Inoue
K
K
Kawakami
Y
Y
D000230:Adenocarcinoma / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001485:Basement Membrane / Q000737:chemistry*; D002401:Cathepsin B / Q000032:analysis*; D003094:Collagen / Q000032:analysis; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007797:Laminin / Q000032:analysis; D008175:Lung Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011379:Prognosis; D015996:Survival Rate
D007797:Laminin; D003094:Collagen; D002401:Cathepsin B