Spin trapping of superoxide in aqueous solutions of fresh and aged cigarette smoke.
Superoxide generation in aqueous solutions of cigarette smoke was determined as a function of the age of smoke using spin trapping. As a spin trap, 5,5-dimethylpyrroline-N-oxide (DMPO) was used. The superoxide adduct of DMPO was detected in a solution of fresh main-stream smoke for over 1 h. The superoxide-generating potential of smoke was rapidly lost as the smoke was kept in a plastic syringe. The smoke that was aged for 3 min did not generate superoxide. Additional evidence of superoxide generation in aqueous solutions of cigarette smoke was obtained by the chemiluminescence method.
Tanigawa
T
T
First Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
Yoshikawa
T
T
Takahashi
S
S
Naito
Y
Y
Kondo
M
M
D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D012906:Smoke / Q000032:analysis*; D012907:Smoking; D012996:Solutions; D013113:Spin Labels; D013481:Superoxides / Q000032:analysis*; D013997:Time Factors; D014867:Water
D003497:Cyclic N-Oxides; D012906:Smoke; D012996:Solutions; D013113:Spin Labels; D014867:Water; D013481:Superoxides; C017245:5,5-dimethyl-1-pyrroline-1-oxide