Alterations in the subcellular distribution of 17 beta-estradiol dehydrogenase in porcine endometrial cells over the course of the estrous cycle.
The uteri of German landrace gilts slaughtered at different days of the cycle were processed for immunocytochemistry and biochemical analyses. Plasma was collected for hormone assays. The monoclonal antibody F1 against the structure-bound 17 beta-estradiol dehydrogenase of porcine endometrial epithelium was applied to rehydrated paraffin sections either as a direct, peroxidase-linked probe or in combination with a fluorescing secondary antibody. The oxidation of estradiol was measured in homogenates of tissue powdered in liquid nitrogen. Immunoreactivity was restricted to endometrial epithelium. In the glandular epithelium, faint dots of fluorescence became visible at day 4, which apparently coalesced to spherical structures of 2-4 microns diameter at the cell basis between days 11 through 17 before disappearing by day 18. A similar distribution was observed for the oxidation products of diaminobenzidine beginning with a faint uniform staining and followed by the appearance of intensely stained basal bodies persisting until day 17. Essentially the same time course was seen in the luminal epithelium but with a different distribution. Immunoreactive material amassed in the apical region of the cells, but the conspicuous aggregations were absent. Time course and intensities of the immunological responses are matched by the enzymatic activity measured in parallel. Both correlate with the plasma progesterone levels, suggesting an induction of the enzyme by the hormone. An involvement of the cytoskeleton in the sequence of subcellular distribution patterns is discussed.
Husen
B
B
Max-Planck-Institut für Experimentelle Endokrinologie, Hannover, Germany.
Adamski
J
J
Szendro
P I
PI
Jungblut
P W
PW
D000818:Animals; D004717:Endometrium / Q000201:enzymology* / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology / Q000502:physiology; D004960:Estradiol Dehydrogenases / Q000032:analysis*; D004971:Estrus / Q000502:physiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D011374:Progesterone / Q000097:blood; D013552:Swine / Q000502:physiology*; D013997:Time Factors
D011374:Progesterone; D004960:Estradiol Dehydrogenases