Cloning and sequencing of phospholipase B gene from the yeast Torulaspora delbrueckii.
The extracellular phospholipase B gene from baker's yeast Torulaspora delbrueckii was cloned and sequenced. Analysis of DNA sequence data revealed an open reading frame (ORF) encoding a 649-amino acid protein, that included amino acid sequences obtained from the purified enzyme. Comparison of these sequence data with the N-terminal amino acid sequence of the enzyme indicated that this secreted protein is synthesized as a large precursor with a 21-amino acid N-terminal extension to the mature enzyme of 628 amino acids. A homology search was carried out between phospholipase B from T. delbrueckii and Penicillium notatum. The deduced amino acid sequence of the cloned phospholipase B was homologous (about 50% identity) to phospholipase B from P. notatum, and contained six conserved regions. The transcriptional level of mRNA of the phospholipase B gene was higher in the cells from early exponential and stationary phases.
Watanabe
Y
Y
Department of Biological Resources, Faculty of Agriculture, Ehime University, Japan.
Yashiki
Y
Y
Sultana
G N
GN
Maruyama
M
M
Kangawa
K
K
Tamai
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D005800:Genes, Fungal / Q000235:genetics*; D008245:Lysophospholipase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D016415:Sequence Alignment; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D015003:Yeasts / Q000201:enzymology / Q000235:genetics*
D015342:DNA Probes; D010446:Peptide Fragments; D012331:RNA, Fungal; D012333:RNA, Messenger; D008245:Lysophospholipase