Differentiation of mouse embryonal carcinoma cells PCC4 by heat shock and the kinetics of induction of heat shock proteins.
Heat shock to embryonal carcinoma cells PCC4 at 45 degrees C for 30 min resulted in the differentiation of cells although heat shock response was induced on exposure to 42 degrees C for 60 min. Differentiated cells were large and well spread with reduced nuclear/cytoplasmic ratios as compared to undifferentiated cells. Change in cell morphology was associated with the disappearance and appearance of stage specific embryonic antigens 1 and 3 respectively. We also found a change in intracellular pH in PCC4 cells within 30 min of heat shock as measured by the change in fluorescence intensity of a probe incorporated into cells during heat shock.
Bisht
K S
KS
Centre for Cellular and Molecular Biology, Hyderabad, India.
Revathi
C J
CJ
Srinivas
U K
UK
D000818:Animals; D018236:Carcinoma, Embryonal / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D007700:Kinetics; D051379:Mice; D014407:Tumor Cells, Cultured
D006360:Heat-Shock Proteins