title: Effect of root conditioning on periodontal wound healing with and without guided tissue regeneration: a pilot study. II. Autoradiographic evaluation.
abstract: This investigation deals with the proliferation and migration of the progenitor cells during the healing of closed periodontal wounds. Periodontal surgical defects affecting the bone and dentin were created in four mongrel dogs. The defects were treated with topical applications of citric acid, tetracycline, or sterile water with and without the placement of nonresorbable membranes. The dogs were killed at 1, 3, 7, and 21 days after surgery. One hour before they were killed, they were intravenously injected with tritiated thymidine. Tissues were processed and routinely prepared for autoradiographic studies. Labeled cells were counted at the apical, coronal, and central areas of the defects. Results suggested that the citric acid and tetracycline treatments inhibited cellular proliferation at the initial time periods of 1 and 3 days. At 7 and 21 days, differences between citric acid and tetracycline treatments were minimal, and neither showed any advantage over the application of sterile water. The placement of the nonresorbable membrane demonstrated a trend of increased labeling at 21 days for all three treatments.
authors.affiliation: Department of Periodontics/Prevention & Geriatrics, University of Michigan, School of Dentistry, Ann Arbor 48109-1078.
authors.forename: P R
authors.initials: PR
authors.lastname: Sammons
authors.forename: H L
authors.initials: HL
authors.lastname: Wang
authors.forename: D J
authors.initials: DJ
authors.lastname: Chiego
authors.forename: W A
authors.initials: WA
authors.lastname: Castelli
authors.forename: B A
authors.initials: BA
authors.lastname: Smith
mesh_terms: D000134:Acid Etching, Dental; D000818:Animals; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal; D010519:Periodontium / Q000502:physiology*; D010865:Pilot Projects; D012038:Regeneration / Q000187:drug effects*; D013754:Tetracyclines / Q000494:pharmacology; D014092:Tooth Root / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D002951:Citrates; D013754:Tetracyclines; D019343:Citric Acid