title: Sialoglycoprotease of Pasteurella haemolytica A1: detection of antisialoglycoprotease antibodies in sera of calves.
abstract: Log phase culture supernate from Pasteurella haemolytica biotype A, serotype 1 contains a proteolytic enzyme specific for O-sialoglycoproteins. Using two methods, Western immunoblotting and enzyme neutralization assay, it was demonstrated that certain bovine sera from two previous P. haemolytica A1 vaccination and challenge trials contained antibodies (Ab) (isotypes IgG1 and IgG2 on Western immunoblot) to the sialoglycoprotease (Gcp). In these trials, selected calves were vaccinated twice with either the commercial culture supernate vaccine Presponse or given phosphate-buffered saline (PBS). One trial was conducted during spring, P. haem XIX, and the other during the winter, P. haem XXI. Although there was no clear evidence for induction of anti-Gcp in response to vaccination, several calves seroconverted following intrapulmonary challenge with live P. haemolytica A1. This is the first report of anti-Gcp Ab in bovine sera. The results indicated that the Gcp is immunogenic and that the bacterium produces the enzyme in vivo. Further, animals with an anti-Gcp response had less pneumonia at necropsy, suggesting the Gcp may induce protective immunity.
authors.affiliation: Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario.
authors.forename: C W
authors.initials: CW
authors.lastname: Lee
authors.forename: P E
authors.initials: PE
authors.lastname: Shewen
authors.forename: W M
authors.initials: WM
authors.lastname: Cladman
authors.forename: J A
authors.initials: JA
authors.lastname: Conlon
authors.forename: A
authors.initials: A
authors.lastname: Mellors
authors.forename: R Y
authors.initials: RY
authors.lastname: Lo
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines / Q000276:immunology; D015153:Blotting, Western; D002417:Cattle; D016978:Mannheimia haemolytica / Q000201:enzymology* / Q000276:immunology; D008666:Metalloendopeptidases / Q000737:chemistry / Q000276:immunology*; D008970:Molecular Weight; D009500:Neutralization Tests; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D008666:Metalloendopeptidases; C070738:O-sialoglycoprotein endopeptidase