Light-regulated translation of chloroplast messenger RNAs through redox potential.
Translation of key proteins in the chloroplast is regulated by light. Genetic and biochemical studies in the unicellular alga Chlamydomonas reinhardtii suggest that light may regulate translation by modulating the binding of activator proteins to the 5' untranslated region of chloroplast messenger RNAs. In vitro binding of the activator proteins to psbA messenger RNA and in vivo translation of psbA messenger RNA is regulated by the redox state of these proteins, suggesting that the light stimulus is transduced by the photosynthesis-generated redox potential.
Danon
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A
Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037.
Mayfield
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SP
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D008027:Light; D008623:Mercaptoethanol / Q000494:pharmacology; D010084:Oxidation-Reduction; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D045332:Photosystem II Protein Complex; D014176:Protein Biosynthesis / Q000187:drug effects; D018747:RNA, Chloroplast / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism / Q000494:pharmacology
D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D018747:RNA, Chloroplast; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D013879:Thioredoxins; D008623:Mercaptoethanol; D004228:Dithionitrobenzoic Acid; D005033:Ethylmaleimide; D004229:Dithiothreitol