Larva lights: a decade of photoaffinity labeling with juvenile hormone analogues.
The introduction of photoaffinity labeling into the mode of action of insect hormones and pheromones started 12 yr ago with the photoaffinity labeling of juvenile hormone binding proteins (JHBPs) from cockroaches in the laboratory of the late John K. Koeppe. Applying this technique to Manduca sexta led ultimately to a three-laboratory collaborative project that has begun to dissect the molecular basis for JH transport, metabolism, and nuclear binding and gene activation in Lepidoptera. This review provides (1) a history of the first experiments; (2) an idea of the breadth of the technique in the arthropod classes Insecta, Crustacea, and Arachnida; and (3) evidence for the depth of the technique in unearthing key details about three different types of the molecular action of JH in M. sexta.
Prestwich
G D
GD
Department of Chemistry, University at Stony Brook, NY 11794-3400.
Touhara
K
K
Riddiford
L M
LM
Hammock
B D
BD
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D007605:Juvenile Hormones; D007814:Larva; D007915:Lepidoptera; D008969:Molecular Sequence Data; D010777:Photochemistry; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid
D000345:Affinity Labels; D007605:Juvenile Hormones