Sterol composition in polyene antibiotic-sensitive and resistant strains of Malassezia pachydermatis.
The membrane sterol compositions of ten strains of Malassezia pachydermatis were analysed by gas-liquid chromatography. Six of these were wild-type strains from clinical sources that were sensitive to polyene antibiotics, whereas the other four strains were resistant mutants that had arisen after treatment with N-methyl-N'-nitrosoguanidine or UV radiation. The wild-type strains contained 838.8 to 1291.7 micrograms of sterol per gram of freeze-dried mycelium. The sterols were composed of 79% ergosterol, 19% fecosterol and 2% lichesterol. The mutant strains contained 277.1 to 348.8 micrograms/g of sterols composed of 76% ergosterol and 24% fecosterol.
Uchida
Y
Y
Department of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Onodera
S
S
Nakade
T
T
Otomo
K
K
D002855:Chromatography, Thin Layer / Q000662:veterinary; D004352:Drug Resistance, Microbial; D008290:Malassezia / Q000737:chemistry* / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D010866:Natamycin / Q000494:pharmacology*; D009761:Nystatin / Q000494:pharmacology*; D013261:Sterols / Q000032:analysis*
D013261:Sterols; D009761:Nystatin; D010866:Natamycin