Disappearance rate of glycosylated and non-glycosylated chicken growth hormone: influence on biological activity.
The disappearance rates for glycosylated (GcGH) and non-glycosylated chicken growth hormone (NGcGH) were compared following their intravenous injection into anaesthetized adult laying hens. The metabolic clearance rate of GcGH was about 20% lower (P = 0.13) compared to NGcGH. Similarly, there was a tendency towards a longer half-life for GcGH. No major physiological significance could be attributed to the glycosylation state of the preparations as far as their effects on circulating insulin-like growth factor I and iodohormone levels were concerned.
Berghman
L R
LR
Laboratory for Neuroendocrinology and Immunological Biotechnology, K.U. Leuven, Belgium.
Buyse
J
J
Huybrechts
L M
LM
Darras
V M
VM
Vandesande
F
F
Kühn
E R
ER
Decuypere
E
E
Scanes
C G
CG
D000704:Analysis of Variance; D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity; D005260:Female; D006031:Glycosylation; D006133:Growth Substances / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D006207:Half-Life; D007275:Injections, Intravenous; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D011863:Radioimmunoassay; D013974:Thyroxine / Q000097:blood*; D014284:Triiodothyronine / Q000097:blood*
D006133:Growth Substances; D014284:Triiodothyronine; D007334:Insulin-Like Growth Factor I; D013974:Thyroxine