Monoclonal antibodies against metalloporphyrins. Specificity of interaction with structurally different metalloporphyrins.
Monoclonal antibodies against Pd-coproporphyrin I have been obtained. The antibody specificity for free as well as for conjugated Pd-coproporphyrin I is characterized. Affinity constants are estimated for 3 monoclonal antibodies effectively interacting with free Pd-coproporphyrin I. A comparative study on the binding of monoclonal antibodies with analogues and derivatives of Pd-coproporphyrin I has revealed that the antigen is mainly located inside the antibody paratope. The protein adjoins complementary to the metalloporphyrin in such a manner that antibodies obtained discern only isomer I, and to some degree, isomer III of coproporphyrin.
Savitsky
A P
AP
A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow.
Demcheva
M V
MV
Mantrova EYu
Ponomarev
G V
GV
D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000918:Antibody Specificity; D003306:Coproporphyrins / Q000276:immunology*; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008665:Metalloporphyrins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D003306:Coproporphyrins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008665:Metalloporphyrins; C031467:coproporphyrin I