Epidermoid carcinoma-cell culture supernatant's inhibition of monocyte chemotaxis: evidence for a tumour-mediated effect.
Epidermoid carcinoma-cell culture supernatants obtained from three laryngeal-epidermoid carcinoma patients were tested in the monocyte suspensions of 13 stage I and stage II (TNM) head and neck epidermoid-carcinoma patients and seven healthy controls for its chemotactic inhibitor activity in vitro with a modified Boyden chamber method. Using 2% casein as the chemoattractant, mean values of monocyte chemotaxis and random migration were found to be decreased in the patient group compared to the controls in a simultaneous assay recorded by the "leading front" method. Simultaneous experiments performed with the supernatant revealed a nearly double-fold increase in random migration and depressed chemotaxis in both groups. The supernatant caused a further depression in the chemotaxis of the patients' already-disabled monocytes, which is thought to be representative for advanced stage tumours. The depression of monocyte chemotaxis, especially in the control group, implies the presence of tumour-derived chemotactic inhibitor(s) for monocytes in the supernatant.
Güngör
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A
Department of Pediatric Hematology, Hacettepe University Faculty of Medicine, Ankara, Turkey.
Yetgin
S
S
Ustacelebi
S
S
Sozeri
B
B
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002465:Cell Movement; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D005260:Female; D006258:Head and Neck Neoplasms / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology*; D014407:Tumor Cells, Cultured