title: Release of monokines by pulmonary macrophages following antigen challenge in sensitized guinea pigs.
abstract: Guinea pigs were passively sensitized with immune serum to ovalbumin (OA), control serum, or saline. Twenty-four hours later, they inhaled aerosols of OA (2% in saline), saline, or lipopolysaccharide (LPS). Following anesthesia, bronchoalveolar lavage (BAL) was performed at 30, 60, 90 and 120 min postinhalation. Alveolar macrophages (AM) were isolated from the BAL fluid and incubated (18 h) in medium alone or with zymosan (1 mg/ml). Supernatants were collected and levels of interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) determined by bioassays. Unstimulated AM from animals that inhaled OA, saline, or LPS secreted similar amounts of IL-1 at 30, 60, and 90 min postinhalation. Zymosan (1 mg/ml) significantly increased IL-1 secretion by AM collected at 60 and 90 min from OA-sensitized animals that inhaled OA or saline. AM from guinea pigs sensitized to OA that inhaled OA or LPS secreted significantly increased amounts of IL-6 at 30, 60, 90, and 120 min postchallenge compared to saline sensitized controls. In all groups, AM from LPS-treated animals secreted large amounts of TNF-alpha at all sampling times postchallenge; AM from OA-sensitized and challenged animals secreted increasing amounts of TNF-alpha with time postchallenge, spontaneously and in response to zymosan. By contrast, AM from saline sensitized and challenged guinea pigs did not release detectable amounts of TNF-alpha spontaneously and secreted very low amounts in the presence of zymosan. These findings show that antigen challenge results in a rapid activation of AM isolated from BAL and suggest AM may initiate the development of inflammatory processes associated with antigen challenge.
authors.affiliation: Pulmonary Research Unit, Faculty of Medecine, University of Sherbrooke, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Denis
authors.forename: D E
authors.initials: DE
authors.lastname: Kelly
authors.forename: D F
authors.initials: DF
authors.lastname: Biggs
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002448:Cell Adhesion; D005260:Female; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D016676:Macrophages, Alveolar / Q000378:metabolism*; D015846:Monokines / Q000378:metabolism*; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000941:Antigens; D007106:Immune Sera; D007375:Interleukin-1; D015850:Interleukin-6; D015846:Monokines; D014409:Tumor Necrosis Factor-alpha; D010047:Ovalbumin; D015054:Zymosan