Concentration of autoantibodies to native 60-kd Ro/SS-A and denatured 52-kd Ro/SS-A in eluates from the heart of a child who died with congenital complete heart block.
OBJECTIVE
To determine the serologic specificity of acid eluates from tissues of a child who died with congenital complete heart block (CCHB).


METHODS
Tissues were extracted, acid eluted, and the IgG and antibody titers determined on the eluates by enzyme-linked immunosorbent assay.


RESULTS
Antibodies to native 60-kd and denatured 52-kd Ro/SS-A were found to be enriched only in the heart eluate, and not in the eluates from brain, kidney, and skin.


CONCLUSIONS
These findings indicate a major role for anti-native 60-kd Ro/SS-A in the immunopathogenesis of CCHB.
Reichlin
M
M
Oklahoma Medical Research Foundation, Oklahoma City 73104.
Brucato
A
A
Frank
M B
MB
Maddison
P J
PJ
McCubbin
V R
VR
Wolfson-Reichlin
M
M
Lee
L A
LA
D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology* / Q000378:metabolism; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005313:Fetal Death / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006327:Heart Block / Q000151:congenital* / Q000276:immunology*; D006801:Humans; D008970:Molecular Weight; D009206:Myocardium / Q000276:immunology*; D011247:Pregnancy; D011489:Protein Denaturation; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000276:immunology* / Q000378:metabolism; D000099207:SS-A Antigen
D001323:Autoantibodies; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human