title: Genetic analysis of liver tumorigenesis in SV40 T antigen transgenic mice implies a role for imprinted genes.
abstract: Liver tumors from interspecific hybrid, transgenic mice containing the SV40 early region linked to a mouse major urinary protein enhancer/promoter were analyzed for loss of heterozygosity to identify chromosomal regions which potentially contain genetic loci involved in multistep tumorigenesis. A broad pattern of complete and partial loss of heterozygosity or allelic imbalance was observed with frequent loss of heterozygosity/partial loss of heterozygosity of loci on chromosomes 1, 5, 7, 8, and 12. In tumors from Mus domesticus x Mus spretus F1 mice a strong preference for loss of the domesticus allele of H19 on chromosome 7 was observed, whereas loss of heterozygosity/partial loss of heterozygosity on chromosome 8 involved preferential loss of spretus alleles. In tumors from reciprocal crosses with Mus castaneus, the maternal chromosome 7 H19 allele was preferentially lost irrespective of whether it was domesticus or castaneus, strongly suggesting the involvement of an imprinted gene(s) in tumor progression.
authors.affiliation: Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263.
authors.forename: W A
authors.initials: WA
authors.lastname: Held
authors.forename: J
authors.initials: J
authors.lastname: Pazik
authors.forename: J G
authors.initials: JG
authors.lastname: O'Brien
authors.forename: K
authors.initials: K
authors.lastname: Kerns
authors.forename: M
authors.initials: M
authors.lastname: Gobey
authors.forename: R
authors.initials: R
authors.lastname: Meis
authors.forename: L
authors.initials: L
authors.lastname: Kenny
authors.forename: Y
authors.initials: Y
authors.lastname: Rustum
mesh_terms: D000483:Alleles; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D015139:Blotting, Southern; D001947:Breeding; D005260:Female; D017353:Gene Deletion; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D000952:Antigens, Polyomavirus Transforming