Local and systemic immune responses to soybean protein ingestion in early-weaned pigs.
The development of local and systemic immune responses to soybean proteins was investigated in early-weaned pigs. Pigs were given either antigenic (ASP, n = 10 pigs) or non-antigenic (NASP, n = 8 pigs) soybean products (6 g of protein/d) from d 5 to 9 of age by stomach tube. After weaning at d 21, pigs were fed diets containing the corresponding soybean products and slaughtered between d 28 to 30. Diarrhea was 2.4-fold more frequent, the size of duodenal villi was reduced by 24 to 36%, and the eosinophil density in the duodenal mucosa was 13 times greater (P < .02) in the ASP pigs compared with the NASP pigs. A larger erythema area (P = .006) was observed in the ASP group than in the NASP group 30 min after an intradermal injection of glycinin, but no significant difference could be detected with alpha- or beta-conglycinin or whole soybean extracts. No difference in skin fold thickness was apparent between groups 24 h later. Intestinal, mesenteric lymph node, and blood lymphocytes did not proliferate when cultured with soybean proteins, regardless of dietary treatment. By ELISA, no difference between groups was observed in the circulating levels of total immunoglobulins (Ig) and IgM. Immunoblotting patterns of raw soybean with sera from 28-d-old pigs showed two bands (22 and 36 kDa) recognized by IgA and IgM, respectively, in the ASP group only. Finally, the adverse effects observed with antigenic soybean flour can be overcome by the use of alcohol-treated products.(ABSTRACT TRUNCATED AT 250 WORDS)
Dréau
D
D
INRA, Laboratoire de Pathologie Infectieuse et Immunologie, centre de Tours, Nouzilly, France.
Lallès
J P
JP
Philouze-Romé
V
V
Toullec
R
R
Salmon
H
H
D000821:Animal Feed; D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000276:immunology; D004386:Duodenum / Q000033:anatomy & histology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004804:Eosinophils / Q000276:immunology; D005260:Female; D015151:Immunoblotting / Q000662:veterinary; D007075:Immunoglobulin M / Q000097:blood; D007136:Immunoglobulins / Q000097:blood; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology; D007428:Intradermal Tests / Q000662:veterinary; D008213:Lymphocyte Activation; D008297:Male; D014674:Plant Proteins, Dietary / Q000008:administration & dosage* / Q000276:immunology; D011897:Random Allocation; D030262:Soybean Proteins; D013025:Glycine max / Q000276:immunology; D013552:Swine / Q000254:growth & development / Q000276:immunology*; D014886:Weaning; D015430:Weight Gain
D004044:Dietary Proteins; D007075:Immunoglobulin M; D007136:Immunoglobulins; D014674:Plant Proteins, Dietary; D030262:Soybean Proteins