title: Depolymerization reactions of hyaluronic acid in solution.
abstract: Samples of microbial sodium hyaluronate were degraded by heating, ultrasonication ultraviolet (UV) and gamma-ray irradiation and enzymatic treatment. The weight-average molecular weight, Mw, of hyaluronate in 0.15 M NaCl and 0.06 M Na2HPO4 was determined by gel filtration with UV detection. The Mw of the degraded samples varied from 8 x 10(4) to 1.38 x 10(6). Depolymerization processes can be described by linear relationship (1/Mw)2 = f(t) in the case of ultrasonic treatment and by non-linear relationships in the cases of heating and UV irradiation at 257 nm. Gamma-ray irradiation and enzymatic treatment caused chemical degradation and depolymerization to oligosaccharides, respectively.
authors.affiliation: Faculty of Chemical Technology, Slovak Technical University, Department of Printing Technology and Applied Photochemistry, Bratislava.
authors.forename: M
authors.initials: M
authors.lastname: Reháková
authors.forename: D
authors.initials: D
authors.lastname: Bakos
authors.forename: M
authors.initials: M
authors.lastname: Soldán
authors.forename: K
authors.initials: K
authors.lastname: Vizárová
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002645:Chickens; D005720:Gamma Rays; D006358:Hot Temperature; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000528:radiation effects; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011108:Polymers; D012996:Solutions; D013010:Sonication; D013291:Streptococcus / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D011108:Polymers; D012996:Solutions; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase