Thapsigargin-induced nuclear calcium signals in rat basophilic leukaemia cells.
By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3) cells. In the presence or absence of external calcium ions, thapsigargin-induced calcium signals were transferred to the nucleus as well as to the cytoplasm of RBL-2H3 cells. The calcium signals were generally much stronger in the nucleus than in the cytoplasm. However, some of the RBL-2H3 cells had apparently reduced nuclear calcium signals. They had a basophil-like bilobed (multilobed) nucleus, although most RBL-2H3 cells had a mast-cell-like monolobed nucleus. In the cells with a bilobed nucleus, IgE-receptor-mediated calcium signals were neither transferred to the nucleus nor to the cytoplasm. The results gave a new insight into the understanding of the mechanism of the nuclear calcium signals in RBL-2H3 cells.
Horikoshi
Y
Y
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Furuno
T
T
Teshima
R
R
Sawada
J
J
Nakanishi
M
M
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D016257:Fura-2; D007942:Leukemia, Experimental / Q000378:metabolism / Q000473:pathology; D018613:Microscopy, Confocal; D051381:Rats; D013729:Terpenes / Q000494:pharmacology*; D019284:Thapsigargin
D013729:Terpenes; D019284:Thapsigargin; D002118:Calcium; D016257:Fura-2