A simple method for obtaining highly viable virus from culture supernatant.
Traditionally density-gradient methods are used to purify viruses. However, these procedures are not only time consuming and cumbersome, recovery of viable viruses are often quite low. In this report, a single-step concentration technique was used to concentrate a mutant of Moloney murine leukemia virus (ts1) virus from culture supernatants by ultrafiltration. A special ultrafiltration unit with a 100,000 mol wt cut-off was able to concentrate viruses about 30-fold without losing any infectivity. In comparison, traditional sucrose density gradient purified viruses lost a significant portion of their infectivity. This technique could be used for concentrating other viruses for many useful purposes where more viable viruses are needed, e.g., study of virus-cell binding.
Saha
K
K
University of Texas, M.D. Anderson Cancer Center, Science Park-Research Division, Smithville 78957.
Lin
Y C
YC
Wong
P K
PK
D002460:Cell Line; D008979:Moloney murine leukemia virus / Q000254:growth & development / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000379:methods*