Immunochemical detection of human lactoferrin in feces as a new marker for inflammatory gastrointestinal disorders and colon cancer.
We have developed a new immunochemical test for fecal lactoferrin (LF) utilizing an enzyme-linked immunosorbent assay (ELISA). The ELISA had a sensitivity of about 10 micrograms/L of lactoferrin and the measurable range was 10.0-1000.0 micrograms/L (1.0-100.0 micrograms LF/g feces). The stability of lactoferrin in feces was greater than that of myeloperoxidase and leucocyte elastase. The fecal concentration of lactoferrin (mean +/- SD) in 35 normal subjects was 0.75 +/- 0.83 microgram/g feces, whereas that in 24 patients with colon cancer was 74.4 +/- 88.3 micrograms/g feces. The fecal lactoferrin concentration of 38 patient with active ulcerative colitis was 307.4 +/- 233.9 micrograms/g feces, and that in 36 patients with active Crohn's disease was 191.7 +/- 231.1 micrograms/g feces. The ELISA for human fecal lactoferrin might be useful in the diagnosis of colon disease.
Uchida
K
K
Kyoto Medical Science Laboratory, Kyoto, Japan.
Matsuse
R
R
Tomita
S
S
Sugi
K
K
Saitoh
O
O
Ohshiba
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D003093:Colitis, Ulcerative / Q000175:diagnosis / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D003111:Colonic Polyps / Q000175:diagnosis / Q000378:metabolism; D003113:Colonoscopy; D003424:Crohn Disease / Q000175:diagnosis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000097:blood / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis
D007781:Lactoferrin