Reconstitution of an operational MHC class II compartment in nonantigen-presenting cells.
Professional antigen-presenting cells (APCs) have a distinct compartment in which class II molecules are proposed to acquire antigenic peptides. Genetic evidence suggests that human leukocyte antigen (HLA)-DM, an unusual class II molecule, participates in this process. Peptide acquisition was reconstituted in nonprofessional APCs by transfection of class II, invariant chain (li), and H-2M, the murine equivalent of DM. The H-2M heterodimer appeared in an endosomal compartment, not at the cell surface, and the localization was independent of li. The data presented show that H-2M, class II, and li are the minimally required components for efficient formation of stable class II-peptide complexes, and thus for a functional class II compartment.
Karlsson
L
L
R. W. Johnson Pharmaceutical Research Institute, Scripps Research Institute, La Jolla, CA 92037.
Péléraux
A
A
Lindstedt
R
R
Liljedahl
M
M
Peterson
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D011992:Endosomes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006183:H-2 Antigens / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015803:HLA-DR3 Antigen / Q000378:metabolism*; D006367:HeLa Cells; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D014162:Transfection
D000944:Antigens, Differentiation, B-Lymphocyte; D006183:H-2 Antigens; D015803:HLA-DR3 Antigen; D000949:Histocompatibility Antigens Class II; C045084:invariant chain