Environmental stress-mediated differential 3' end formation of chloroplast RNA-binding protein transcripts.
We report the characterization of transcripts from the halophyte, Mesembryanthemum crystallinum, encoding a protein with high homology to chloroplast RNA-binding proteins (cRBP). In this plant chloroplast-related functions are largely protected against salt stress. cRBP transcripts are derived from a single gene, Mc32crbp, although three size classes of polyadenylated mRNAs are detected. Transcription rate and steady state amounts of mRNA are developmentally regulated and light controlled with strong transcriptional activity as functional chloroplasts are established, and with lower maintenance activity thereafter. Upon salt stress, the rate of transcription decreases, although transcript levels increase. Accompanying stress, a change in the distribution of transcript size classes is observed as the longest transcript with an untranslated 3' end of 381 nucleotides increases relative to transcripts with shorter 3' ends. The long transcript is characterized by the presence of five sequence elements in the 3'-untranslated region that are present in cRBP mRNAs from a variety of plants, although not all elements are found in each mRNA. The results may indicate a mechanism by which mRNA levels of constitutively light-regulated genes may be modulated without enhanced transcription in response to environmental cues.
Breiteneder
H
H
Department of Biochemistry, University of Arizona, Tucson 85721.
Michalowski
C B
CB
Bohnert
H J
HJ
D000222:Adaptation, Physiological / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D060365:Chloroplast Proteins; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D008969:Molecular Sequence Data; D063245:Plant Development; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics; D016133:Polymerase Chain Reaction; D018747:RNA, Chloroplast / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D012492:Salts; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014158:Transcription, Genetic
D060365:Chloroplast Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; D018747:RNA, Chloroplast; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012492:Salts