title: Sequences of heavy and light chain variable regions from four bovine immunoglobulins.
abstract: Oligodeoxyribonucleotide primers based on the 5' ends of bovine IgG1/2 and lambda constant (C) region genes, together with primers encoding conserved amino acids at the N-terminus of mature variable (V) regions from other species, have been used in cDNA and polymerase chain reactions (PCRs) to amplify heavy and light chain V region cDNA from bovine heterohybridomas. The amino acid sequences of VH and V lambda from four bovine immunoglobulins of different specificities are presented.
authors.affiliation: Scotgen Biopharmaceuticals Inc, Bridge of Don, Aberdeen, UK.
authors.forename: K L
authors.initials: KL
authors.lastname: Armour
authors.forename: P R
authors.initials: PR
authors.lastname: Tempest
authors.forename: P H
authors.initials: PH
authors.lastname: Fawcett
authors.forename: M L
authors.initials: ML
authors.lastname: Fernie
authors.forename: S I
authors.initials: SI
authors.lastname: King
authors.forename: P
authors.initials: P
authors.lastname: White
authors.forename: G
authors.initials: G
authors.lastname: Taylor
authors.forename: W J
authors.initials: WJ
authors.lastname: Harris
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000914:Antibodies, Viral / Q000737:chemistry; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry* / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012136:Respiratory Syncytial Viruses / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains