Roles of the splenic cytokines and arachidonic acid metabolites in severe hepatic injury after lipopolysaccharide injection in chronically alcohol-fed rats.
We examined the early changes after lipopolysaccharide (LPS) injection in chronically alcohol-fed rats with or without splenectomy. Administration of 2 mg/kg body weight LPS caused severe hepatic injury. The plasma aspartate aminotransferase (ASAT) activity was significantly higher in sham-operated rats 8 hr after LPS injection than in splenectomized rats. The plasma tumor necrosis factor (TNF) activity was also significantly higher 1 hr after LPS injection in sham-operated rats than in splenectomized rats. The plasma thromboxane (TX) B2/6-keto-prostaglandin (PG) F1 alpha ratio increased in sham-operated rats after LPS injection. Therefore, the balance of arachidonic acid metabolites was in a hypercoagulated state in sham-operated rats after the LPS injection. Neutrophil infiltration into liver tissue increased in sham-operated rats after the LPS injection. The cytokines and arachidonic acid metabolites released from the spleen after LPS injection in alcohol-fed rats may play important roles in severe hepatic injury.
Furudera
S
S
Second Department of Medicine, Kurume University School of Medicine, Fukuoka, Japan.
Kumashiro
R
R
Tanaka
S
S
Ishii
K
K
Koga
Y
Y
Hamada
T
T
Hisatomi
S
S
Sata
M
M
Tanikawa
K
K
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D016207:Cytokines / Q000502:physiology*; D004731:Endotoxins / Q000097:blood; D004926:Escherichia coli / Q000276:immunology; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000276:immunology*; D008099:Liver / Q000276:immunology / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000276:immunology* / Q000473:pathology; D008111:Liver Function Tests; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D009504:Neutrophils / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects* / Q000276:immunology; D013156:Splenectomy
D016207:Cytokines; D004731:Endotoxins; D008070:Lipopolysaccharides; D016718:Arachidonic Acid