title: Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans.
abstract: A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 x 10(2) to 2.5 x 10(4) transformants per 10(6) viable protoplasts and microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants.
authors.affiliation: Department de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Moreno
authors.forename: C
authors.initials: C
authors.lastname: Pascual
authors.forename: A
authors.initials: A
authors.lastname: Gibello
authors.forename: S
authors.initials: S
authors.lastname: Ferrer
authors.forename: C J
authors.initials: CJ
authors.lastname: Bos
authors.forename: A J
authors.initials: AJ
authors.lastname: Debets
authors.forename: G
authors.initials: G
authors.lastname: Suárez
mesh_terms: D001230:Aspergillus / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics*; D004261:DNA Replication; D004271:DNA, Fungal / Q000032:analysis; D008938:Mitosis; D010957:Plasmids / Q000235:genetics; D011523:Protoplasts; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal