title: Developmental and tissue-specific expression of prosaposin mRNA in murine tissues.
abstract: Prosaposin is a multifunctional locus in humans and mice that encodes in tandem and in the same reading frame four glycoprotein activators, or saposins, of lysosomal hydrolases. These ubiquitously expressed glycoproteins and the precursor, prosaposin, have been proposed to function in glycosphingolipid catabolic pathways and glycolipid transport. To characterize the temporal and spatial expression of the prosaposin locus, prenatal and postnatal mouse tissues were screened by in situ hybridization with a mouse antisense riboprobe for prosaposin. Prenatally, prosaposin mRNA was expressed differentially in the placenta and prominently in the decidua basalis and capsularis where expression was gestational age dependent. No other region of high-level expression was detectable in the prenatal mouse. In comparison, high-level differential expression of prosaposin was clearly evident postnatally in a variety of organs, including secretory epithelial cells of the choroid plexus, ependymal lining, upper trachea, esophagus, cortical tubules of the kidney, sertoli cells of the testes and epididymis. Discrete localization of prosaposin mRNA expression was also found in neurons of the cerebral cortex, cerebellar cortex, and lateral columns of the spinal cord as well as in hepatocytes of the mature liver. Very high levels of expression were found in specialized tissues including the Harderian glands and macrophages of lymph nodes, lungs, splenic tissue, and thymus. These studies indicate that the expression of the prosaposin locus, a presumed "housekeeping" gene, is under tissue- and cell-specific differential control. The spatial organization of expression suggests a role for this locus in the expression of glycosphingolipid-storage diseases.
authors.affiliation: Division of Human Genetics, Children's Hospital Medical Center, Cincinnati, Ohio 45229-3039.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.forename: D P
authors.initials: DP
authors.lastname: Witte
authors.forename: G A
authors.initials: GA
authors.lastname: Grabowski
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000378:metabolism / Q000502:physiology; D005314:Embryonic and Fetal Development; D005260:Female; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D049231:Saposins; D014018:Tissue Distribution
chemical_list: D006023:Glycoproteins; C483345:PSAP protein, human; D011498:Protein Precursors; C483344:Psap protein, mouse; D012333:RNA, Messenger; D049231:Saposins