Polyclonal antibody against a complement-activating pectin from the roots of Angelica acutiloba.
Anti-sera against a complement-activating pectin (AR-2IIb), which was purified from the roots of Angelica acutiloba Kitagawa, were obtained by immunization of rabbits, and a polyclonal anti-AR-2IIb antibody of the IgG class was purified by affinity chromatography on AR-2IIb-immobilized Sepharose and Protein G-Sepharose. Periodate oxidation of AR-2IIb significantly reduced its inhibitory activity on the reactivity of AR-2IIb to anti-AR-2IIb-IgG, but pronase digestion of AR-2IIb did not affect its inhibitory activity. Other pharmacologically active pectins from A. autiloba, Bupleurum falcatum, and Glycyrrhiza uralensis and the complement-activating pectic arabinogalactan from A. autiloba also showed significant inhibitory activities on the reactivity of AR-2IIb to anti-AR-2IIb-IgG, but these inhibitory activities were lower than that of AR-2IIb. Other pectins, polygalacturonic acid, arabinogalactan, galactan, and araban tested had negligible inhibitory activity. Endo-a-(1-->4)-polygalacturonase digestion of AR-2IIb indicated that its "ramified" region (rhamnogalacturonan core possessing neutral oligosaccharide side-chains) contained epitopes for anti-AR-2IIb-IgG, but that 2-keto-3-deoxyoctulosonic acid (KDO)-containing regions and oligogalacturonides obtained from AR-2IIb were not recognized by anti-AR-2IIb-IgG. Although carboxyl-reduction of galacturonic acid in the "ramified" region decreased the inhibitory activity of the "ramified" on its reactivity to anti-AR-2IIb, an acidic tetrasaccharide unit in the rhamnogalacturonan core had negligible inhibitory activity.
Wang
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NL
Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
Kiyohara
H
H
Matsumoto
T
T
Otsuka
H
H
Hirano
M
M
Yamada
H
H
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D003167:Complement Activation; D007074:Immunoglobulin G / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010368:Pectins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D018517:Plant Roots; D010946:Plants, Medicinal; D011817:Rabbits / Q000276:immunology
D000906:Antibodies; D007074:Immunoglobulin G; D010368:Pectins