Antibodies from egg yolk of immunized hens against a bioactive caseinopeptide (beta-casokinin-10).
Antibodies (IgY) directed against a synthetic, bioactive peptide (beta-casokinin-10) were obtained from egg yolk of immunized chickens. Using a beta-casokinin-10/BSA conjugate for immunization, large quantities of high-titered anti-peptide antibodies were obtained. ELISA standard curves for beta-casokinin-10 were linear in the range 30-22,000 ng/ml. IgY-antibodies against beta-casokinin-10 recognized not only the immunogenic peptide structure but also analogues epitopes in protein preparations containing bovine beta- and alpha s-caseins, respectively, as well as in ovine caseins. The anti-beta-casokinin-10 IgY-antibodies are intended to be used as immunochemical reagents in future structure-activity studies of bioactive casokinins that are inhibitors of the angiotensin-converting enzyme.
Meisel
H
H
Institut für Chemie und Physik, Bundesanstalt für Milchforschung, Kiel, Germany.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D002364:Caseins / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D002417:Cattle; D002645:Chickens; D002846:Chromatography, Affinity; D004530:Egg Yolk / Q000737:chemistry / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007120:Immunochemistry; D007136:Immunoglobulins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology*; D012756:Sheep; D013329:Structure-Activity Relationship
D000596:Amino Acids; D000806:Angiotensin-Converting Enzyme Inhibitors; D000906:Antibodies; D002364:Caseins; C016512:IgY; D007136:Immunoglobulins; D010446:Peptide Fragments; C090776:beta-casokinin 10