title: Nucleotide sequence of the gene for peptostreptococcal protein L.
abstract: A gene bank of Peptostreptococcus magnus DNA was established using an E. coli host-vector system. Western blot analysis identified a clone expressing protein L which bound to the light chain of human immunoglobulins. DNA sequence determination and analysis revealed an open reading frame of 992 amino acids, giving a theoretical secreted protein of 106 kD with a pl of 4.67.
authors.affiliation: Division of Biotechnology, PHLS Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.
authors.forename: J P
authors.initials: JP
authors.lastname: Murphy
authors.forename: A R
authors.initials: AR
authors.lastname: Trowern
authors.forename: C J
authors.initials: CJ
authors.lastname: Duggleby
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D015723:Gene Library; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010462:Peptostreptococcus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C055047:Ig L-binding protein, Peptostreptococcus