Origin of the N-1, C-2 and C-2' atoms of the pyrimidine moiety of thiamin in Saccharomyces cerevisiae.
The precursor of the pyrimidine moiety of thiamin in S. cerevisiae was studied. The tracers [15N]- and [2'-13C]-pyridoxine were chemically synthesized, and incorporated efficiently into the pyrimidine. The mass fragmentation pattern showed that the nitrogen atom of pyridoxine was incorporated into the N-1 of the pyrimidine. In addition, the C-2' atom of pyridoxine was incorporated into the C-2' atom of the pyrimidine. These results and the structural resemblance between the pyrimidine and pyridoxine revealed that the C-2, C-2' and N-1 unit of the pyrimidine are derived from the C-2, C-2' and N-1 unit of pyridoxine.
Tazuya
K
K
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
Azumi
C
C
Yamada
K
K
Kumaoka
H
H
D002244:Carbon / Q000378:metabolism*; D002247:Carbon Isotopes; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes; D011743:Pyrimidines / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013831:Thiamine / Q000096:biosynthesis*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011743:Pyrimidines; D002244:Carbon; D009584:Nitrogen; D013831:Thiamine