[Glutathione S transferases-pi].
It has been known that many drug resistant factors including p-glycoprotein related to anticancer drug resistance. It is assumed that Glutathione s-transferase (GST) is one of the resistant factors. In this present study, we examined the relationship between GST (especially GST-pi) and drug resistance, and also possibility of overcoming of drug resistance for GST-pi related drug resistance. We studied whether GST-pi directly related to anticancer drug resistance by transfection of GST-pi antisense cDNA into human colonic cancer cell line (M 7609). By transfection, cytosolic GST-pi concentrations decreased and sensitivity for adriamycin increased. It was confirmed that GST-pi directly related to some anticancer drug resistance including adriamycin. Moreover, we also have found that ketoprofen, which is an inhibitor of GST-pi activity, increased Adriamycin sensitivity. That is, partial overcoming of drug resistance was obtained. In future, it will be expected that GST-pi inhibitors etc are tried for overcoming of drug resistance.
Takahashi
Y
Y
Dept. of Internal Medicine, Sapporo Medical University School of Medicine.
Niitsu
Y
Y
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D016375:Antisense Elements (Genetics); D003110:Colonic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D004317:Doxorubicin / Q000494:pharmacology; D004351:Drug Resistance / Q000235:genetics; D005982:Glutathione Transferase / Q000097:blood / Q000502:physiology*; D006801:Humans; D007527:Isoenzymes / Q000097:blood / Q000502:physiology*; D007660:Ketoprofen / Q000494:pharmacology; D051381:Rats; D014407:Tumor Cells, Cultured / Q000201:enzymology
D000970:Antineoplastic Agents; D016375:Antisense Elements (Genetics); D007527:Isoenzymes; D004317:Doxorubicin; D007660:Ketoprofen; D005982:Glutathione Transferase