Expression of bone morphogenetic protein genes in the human dental pulp cells.
Dental pulp has a potential to induce ectopic bone formation, but little is known about its mechanism. We thought that bone morphogenetic proteins (BMPs), members of the transforming growth factor-beta (TGF-beta) superfamily, are involved in the osteoinductive activity of dental pulp. In order to prove this assumption, we constructed a cDNA library from primary culture cells of human dental pulp (HDP cells), and screened the library with previously cloned cDNAs for mouse BMP-2 and -6 as probes. Three distinct cDNA clones encoding human BMP-2, -4 and -6 were isolated. By Northern blot analysis, specific transcripts of the genes of those BMPs were detected in the HDP cells. It was concluded that the BMPs were expressed in a certain population of dental pulp cells and might play some roles in ectopic bone formation by dental pulp.
Takeda
K
K
First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Oida
S
S
Goseki
M
M
Iimura
T
T
Maruoka
Y
Y
Amagasa
T
T
Sasaki
S
S
D000293:Adolescent; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D019485:Bone Morphogenetic Proteins; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D015870:Gene Expression / Q000235:genetics*; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics
D019485:Bone Morphogenetic Proteins; D018076:DNA, Complementary; D006133:Growth Substances; D009363:Neoplasm Proteins; D011506:Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta