Molecular cloning and sequence analysis of cDNA coding for the precursor of the human salivary proline-rich peptide P-B.
cDNA coding for the human salivary proline-rich peptide P-B was amplified by polymerase chain reaction using human submaxillary gland cDNA as the template and d(deoxy)T25 as the primer for both strands. The amplified cDNA of 0.8 kbp encodes a hydrophobic signal sequence of 22 amino acid residues and the complete sequence of P-B (57 amino acids). The determined nucleotide sequence of P-B cDNA indicates that P-B is a mature full-size protein and not the enzymic degradation product of a larger protein. The gene coding for P-B is not a member of the proline-rich protein gene family characterized previously.
Isemura
S
S
Nippon Dental University Junior College at Niigata.
Saitoh
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005815:Genetic Code; D016678:Genome; D016681:Genome, Fungal; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D011392:Proline / Q000235:genetics; D055232:Proline-Rich Protein Domains; D011498:Protein Precursors / Q000235:genetics*; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013363:Submandibular Gland / Q000737:chemistry
D018076:DNA, Complementary; D010455:Peptides; D011498:Protein Precursors; D012471:Salivary Proteins and Peptides; D011392:Proline