title: Transcriptional regulation of the Trichoderma longibrachiatum egl1 gene.
abstract: Transcription of the Trichoderma longibrachiatum egl1 gene is induced in the presence of lactose and beta-methylglucoside and repressed by glucose. A DNA fragment containing 722 bp upstream of the ATG codon has been sequenced. The gene has two major transcription start points (20 and 24 nucleotides upstream from the ATG codon) and several transcription termination points (located in a region around 130 nt downstream of the stop codon). Two 6-mer sequences (5'-CTGGAG-3') separated by 16 bp are present in the egl1 gene promoter. These sequences match the Aspergillus nidulans consensus CreA binding site and might be implicated in carbon catabolite repression of egl1 transcription.
authors.affiliation: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de los Alimentos (CSIC), Valencia, Spain.
authors.forename: R
authors.initials: R
authors.lastname: González
authors.forename: J A
authors.initials: JA
authors.lastname: Pérez-González
authors.forename: L
authors.initials: L
authors.lastname: González-Candelas
authors.forename: D
authors.initials: D
authors.lastname: Ramón
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002480:Cellulase / Q000235:genetics*; D004794:Enzyme Repression; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D007785:Lactose / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism; D013728:Terminator Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D008757:Methylglucosides; D012097:Repressor Proteins; C071237:CreA protein, Aspergillus nidulans; C027020:methylglucoside; D002480:Cellulase; C090774:endoglucanase EGL1; D007785:Lactose