title: Sperm-immunobead binding decreases with in vitro incubation.
abstract: OBJECTIVE
  To characterize the stability of the sperm-immunobead association over time.
  
  
  METHODS
  Prospective evaluation of sperm-immunobead binding, using direct and indirect assays.
  
  
  METHODS
  Male Infertility Clinic, University of California, Davis, Davis, California.
  
  
  METHODS
  Eleven men with sperm surface antibodies and 25 men with serum antisperm antibodies volunteered.
  
  
  METHODS
  Repeated assessment of sperm-immunobead binding over time.
  
  
  RESULTS
  Serum immunoglobulin (Ig)G decreased a mean of 42.6% over 30 minutes, and serum IgA decreased a mean of 22.7% over 30 minutes. Semen-derived IgG binding fell a mean of 59.9% and semen-derived IgA fell a mean of 27.0% over 25 to 40 minutes.
  
  
  CONCLUSIONS
  Sperm-immunobead coincubation results in a decrease in the number of sperm bound to immunobeads.
authors.affiliation: University of California, School of Medicine, Davis.
authors.forename: J E
authors.initials: JE
authors.lastname: Gould
authors.forename: C K
authors.initials: CK
authors.lastname: Brazil
authors.forename: J W
authors.initials: JW
authors.lastname: Overstreet
mesh_terms: D000906:Antibodies / Q000032:analysis*; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007158:Immunologic Techniques; D008297:Male; D008863:Microspheres; D011446:Prospective Studies; D013094:Spermatozoa / Q000276:immunology*; D013997:Time Factors
chemical_list: D000906:Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M