title: Oncogene arrangement in a shooty strain of Agrobacterium tumefaciens.
abstract: The Agrobacterium tumefaciens nopaline strain 82.139 induces non-teratogenic shooty tumours on several plant species. We have determined the position of the T-region oncogenes in a 11.4 kb Xba I fragment which shows a general organization similar to its pTiC58 counterpart. Sequence analysis of the 4.7 kb right part of this fragment allowed us to identify the pTi82.139 ipt, 6b and nos coding sequences. pTi82.139 lacks the 6a gene, which lies between the ipt and 6b genes in pTiC58. The intervening region between the 6b and the nos genes contains an additional ORF with homology to ORF 21 (transcript 3') from the TR-DNA of octopine strain pTi15955.
authors.affiliation: Laboratoire de Biologie Cellulaire, INRA, Versailles, France.
authors.forename: C
authors.initials: C
authors.lastname: Drevet
authors.forename: A C
authors.initials: AC
authors.lastname: Brasileiro
authors.forename: L
authors.initials: L
authors.lastname: Jouanin
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D016366:Open Reading Frames; D010802:Phylogeny; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific