title: Ejaculation induced changes in escape latency in the hot plate test: pharmacological analysis of anxiolytic versus analgesic effect.
abstract: The possible changes in nociception at various stages of male sexual behaviour were explored in the hot plate test. Although other authors have reported an antinociceptive effect of mating, we failed to find this effect on the hot plate test after several sexual behaviour events. To further explore the possible antinociceptive action of copulation we administered a suboptimal analgesic dose of morphine (0.3 mg/kg i.p.). No change in nociception were observed in animals treated with a subthreshold dose of morphine and tested in the nociception test after ejaculation were observed. Since previous reports have refered that ejaculation produces hypoalgesia when measured in the hot plate test, we attempted to replicate these findings. We found that one ejaculation produces an increase in the latency to escape in male rats previously habituated to the hot plate test. These results suggest a hypoalgesic effect. However, such changes could also be interpreted as alterations in the animals' emotionality. Thus, a group of habituated animals was tested on the switched off plate. An important increase in the escape latency behaviour after ejaculation was observed, while no differences between control animals, without sexual behaviour display, tested on switched on and switched off plate were observed. The participation of the benzodiazepine and opiod systems in the ejaculation effect on the switched off plate was explored. A similar increase in escape latency to that induced by ejaculation was caused by diazepam (1.0 and 2.0 mg/kg). The increase in escape latency induced by ejaculation was prevented by the benzodiazepine antagonist, flumazenil (20.0 mg/kg).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: División de Investigaciones en Neurociencias, Instituto Mexicano de Psiquiatría, México, DF.
authors.forename: A
authors.initials: A
authors.lastname: Saldívar-González
authors.forename: A
authors.initials: A
authors.lastname: Fernández-Guasti
mesh_terms: D000818:Animals; D001143:Arousal / Q000187:drug effects*; D003307:Copulation / Q000187:drug effects; D003975:Diazepam / Q000494:pharmacology*; D004542:Ejaculation / Q000187:drug effects*; D004924:Escape Reaction / Q000187:drug effects*; D005260:Female; D005442:Flumazenil / Q000494:pharmacology; D006185:Habituation, Psychophysiologic / Q000187:drug effects; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009270:Naloxone / Q000494:pharmacology; D017288:Pain Threshold / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000187:drug effects*; D013697:Thermosensing / Q000187:drug effects*; D014828:Vocalization, Animal / Q000187:drug effects
chemical_list: D009270:Naloxone; D005442:Flumazenil; D009020:Morphine; D003975:Diazepam