Chlorpromazine attenuated electroacupuncture analgesia in conscious rabbits.
By measuring the defense behavior in response to the noxious stimulation induced by potassium iontophoresis on ear-lobe skin of conscious rabbit, chlorpromazine (CPZ) (0.5 mg.kg-1, i.v.) induced hyperalgesia, whereas it significantly attenuated electroacupuncture analgesia (EAA) efficacy. Monoamines and their metabolites in cerebrospinal fluid (CSF) were measured by high pressure liquid chromatography with electrochemical detector (HPLC-ECD) while the attenuation effect of CPZ on EAA was observed. CPZ markedly enhanced 3,4-dihydroxyphenylacetic acid (DOPAC) (P < 0.05) and homovanillic acid (HVA) (P < 0.01) contents in CSF both in the presence and absence of electroacupuncture. CPZ attenuated EAA with elevations of either DOPAC or HVA concentrations in CSF. There was a positive correlation between the increase of DOPAC or HVA content in CSF and the attenuation effect of CPZ on EAA (P < 0.05). These results suggested that the activation of dopamine system was unfavorable for EAA.
Dai
J L
JL
Department of Neurobiology, School of Basic Medical Sciences, Shanghai Medical University, China.
Xu
S F
SF
D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid; D015667:Acupuncture Analgesia; D000818:Animals; D015306:Biogenic Monoamines / Q000134:cerebrospinal fluid; D002746:Chlorpromazine / Q000494:pharmacology*; D015671:Electroacupuncture; D005260:Female; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid; D008297:Male; D017288:Pain Threshold / Q000187:drug effects*; D011817:Rabbits
D015306:Biogenic Monoamines; D015102:3,4-Dihydroxyphenylacetic Acid; D002746:Chlorpromazine; D006719:Homovanillic Acid