Is the binding of mistletoe lectins I and III a useful prognostic indicator in colorectal carcinoma?
A retrospective study on 127 patients with colorectal carcinoma was performed to evaluate the use of mistletoe lectins I and III as possible prognostic indicators. Tissue sections were stained by a histochemical technique using these lectins and the staining results correlated with survival. No correlation between survival and the presence of lectin binding or non-binding was found. A lectin with the same monosaccharide specificity as ML-III, Helix pomatia agglutinin, which was of prognostic significance in the same patient cohort in another study showed on Western blot analysis a slightly different lectin binding pattern towards glycoproteins than the MLs. The results of this study indicate that even subtle differences in the carbohydrate composition of glycoconjugates which can be differentiated by lectins are of biological significance in terms of the metastatic spread of tumours.
Dixon
A
A
University of Southampton, UK.
Schumacher
U
U
Pfüller
U
U
Taylor
I
I
D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D028321:Plant Preparations; D010940:Plant Proteins; D011237:Predictive Value of Tests; D011379:Prognosis; D011485:Protein Binding; D012189:Retrospective Studies; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000378:metabolism*
D006023:Glycoproteins; D037102:Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C488864:mistletoe lectin III; C009674:ribosome inactivating protein, Viscum; D054788:Ribosome Inactivating Proteins