Hematoporphyrin derivative (Photofrin) photodynamic action on Ca2+ transport in monkey kidney cells (CV-1).
After 24 h incubation with Photofrin (PF), photodynamic action has been studied on Ca2+ transport in CV-1 cells. A moderate increase of the cytosolic free Ca2+ concentration [Ca2+]i is observed immediately after a dose of irradiation which yields a survival rate of less than 5% at 48 h. In parallel, studies on digitonin-permeabilized cells indicate that such a treatment inhibits endoplasmic reticulum Ca2+ uptake with few alterations of this process in mitochondria. In contrast, ADP-stimulated respiration is impeded and intracellular ATP level decreases. It is suggested that direct damage to endoplasmic reticulum as well as mitochondrial disturbance are the primary mechanisms responsible for a nontransient elevation of [Ca2+]i preceding cell death.
Dellinger
M
M
Laboratoire de Biophysique, INSERM U 201 et CNRS URA 481, Muséum National d'Histoire Naturelle, Paris, France.
Ricchelli
F
F
Moreno
G
G
Salet
C
C
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D017324:Hematoporphyrin Derivative / Q000494:pharmacology*; D007668:Kidney; D007700:Kinetics; D010101:Oxygen Consumption / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D017324:Hematoporphyrin Derivative; D000255:Adenosine Triphosphate; D002118:Calcium