title: The legumin gene family: a reconstructed Vicia faba legumin gene encoding a high-molecular-weight subunit is related to type B genes.
abstract: Nucleotide sequence information from a partial genomic clone, a cDNA clone, a RACE clone and a PCR fragment was combined to reconstruct the first reported complete gene sequence encoding a large legumin subunit, designated LelB3. The length difference to the well-characterized major legumin subunits is caused by an extended glutamine/glutamic acid-rich region encoded by the C-terminal part of the alpha chain. Amino acid sequence comparisons reveal that gene LelB3 is more closely related to B-type than to A-type legumin genes of Vicia faba. Gene LelB3 is a member of a small gene family as indicated by published (Pich and Schubert, Biol Zbl 112 (1993); 342-350) and limited own data.
authors.affiliation: Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Heim
authors.forename: H
authors.initials: H
authors.lastname: Bäumlein
authors.forename: U
authors.initials: U
authors.lastname: Wobus
mesh_terms: D000595:Amino Acid Sequence; D007887:Fabaceae / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D010446:Peptide Fragments / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal; D017386:Sequence Homology, Amino Acid; D000097793:Legumins
chemical_list: D010446:Peptide Fragments; D010940:Plant Proteins; D000097793:Legumins