Abdominal rubber drain piece aggravates intra-abdominal sepsis in the rat.
Biomaterials in the peritoneal cavity disrupt the physiology of the host and may cause bacterial translocation. The current study was performed to determine whether biomaterials exacerbate intra-abdominal infections. Adult male rats were divided into four groups: group 1, celiotomy+intraperitoneal (i.p.) saline; group 2, celiotomy+i.p. Escherichia coli (3 x 10(8) cfu); group 3, i.p. rubber+i.p. saline; and group 4, i.p. rubber+i.p. E. coli (3 x 10(8) cfu). Twelve h after the challenge, enteric bacterial translocation, bacterial population levels in the cecum and serum levels of IL-6 and TNF were measured. Bacterial translocation to mesenteric lymph nodes and the liver was observed in animals from groups 2 and 3, but significantly increased in group 4 with a concomitant elevation of serum levels of TNF and IL-6, as compared with group 1. Histological examination revealed a more pronounced inflammatory reaction in the peritoneum and distal ileum in group 4 than in groups 2 and 3. These results suggest that the presence of rubbers in the peritoneal cavity aggravates intra-abdominal sepsis.
Guo
W
W
Department of Surgery, Lund University, Sweden.
Soltesz
V
V
Ding
J W
JW
Willén
R
R
Liu
X
X
Andersson
R
R
Bengmark
S
S
D000005:Abdomen / Q000601:surgery; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects*; D002465:Cell Movement; D004322:Drainage / Q000009:adverse effects*; D004755:Enterobacteriaceae / Q000472:pathogenicity; D006400:Hematocrit; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D010537:Peritoneum / Q000473:pathology; D010538:Peritonitis / Q000150:complications*; D019736:Prostheses and Implants / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012408:Rubber / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D001672:Biocompatible Materials; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D012408:Rubber