title: Opposing roles for dopamine D2 and D3 receptors on neurotensin mRNA expression in nucleus accumbens.
abstract: Using in situ hybridization histochemistry in rat nucleus accumbens, we show that the dopamine D3 receptor mRNA is expressed in the ventromedial part of the shell subdivision, where its gross distribution matches that of neurotensin mRNA. In addition, hybridization studies at the cellular level show that a large fraction of the neurotensin neurons co-express the D3 receptor mRNA in this restricted area. In contrast, the dopamine D2 receptor mRNA is expressed mainly in the core and marginally in the shell, at the level of the cone. In rats treated by haloperidol and sulpiride, two D2-like receptor antagonists, but not by SCH 23390, a D1-like receptor antagonist, proneurotensin mRNA was increased in the D2 receptor mRNA-rich areas but decreased in the D3 receptor mRNA-rich areas. This suggests that the D2 and D3 receptors control neurotensin mRNA expression negatively and positively, respectively.
authors.affiliation: Unité de Neurobiologie et de Pharmacologie de I'INSERM, Centre Paul Broca, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Diaz
authors.forename: D
authors.initials: D
authors.lastname: Lévesque
authors.forename: N
authors.initials: N
authors.lastname: Griffon
authors.forename: C H
authors.initials: CH
authors.lastname: Lammers
authors.forename: M P
authors.initials: MP
authors.lastname: Martres
authors.forename: P
authors.initials: P
authors.lastname: Sokoloff
authors.forename: J C
authors.initials: JC
authors.lastname: Schwartz
mesh_terms: D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D009496:Neurotensin / Q000096:biosynthesis*; D009714:Nucleus Accumbens / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D011954:Receptors, Dopamine / Q000502:physiology*; D017448:Receptors, Dopamine D2 / Q000502:physiology*; D050637:Receptors, Dopamine D3; D013469:Sulpiride / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D001552:Benzazepines; C493595:Drd3 protein, rat; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D009496:Neurotensin; D013469:Sulpiride; D006220:Haloperidol