title: Uptake of exogenous fluorescent Di-I by intact junctional epithelium of adult rats allows retrograde labeling of trigeminal sensory neurons.
abstract: Junctional epithelium (JE) is the special attachment tissue between gingiva and teeth, and it is well innervated by sensory nerve fibers. We have found that the fluorescent carbocyanine dye, Di-I, can penetrate quickly into intact JE and spread into connective tissue. Di-I containing neurons in trigeminal ganglion were found at 3-7 days and were mostly gone by 3 weeks. We conclude that substances such as Di-I can penetrate through permeable epithelia such as intact JE where they are picked up by sensory nerve fibers and carried to nerve cell bodies.
authors.affiliation: Department of Anesthesiology, University of Washington, Seattle 98195.
authors.forename: A
authors.initials: A
authors.lastname: Sugaya
authors.forename: E H
authors.initials: EH
authors.lastname: Chudler
authors.forename: M R
authors.initials: MR
authors.lastname: Byers
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000493:pharmacokinetics*; D004058:Diffusion; D004846:Epithelial Attachment / Q000378:metabolism*; D005456:Fluorescent Dyes; D008297:Male; D008856:Microscopy, Fluorescence; D009475:Neurons, Afferent / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D012668:Trigeminal Ganglion / Q000166:cytology / Q000378:metabolism*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine