Free radical-induced double base lesions.
Evidence is presented for the formation of products in irradiated dinucleoside monophosphates in which both bases are damaged. The dinucleoside monophosphates d(GpT), d(GpC), d(TpG) and d(CpG) were X-irradiated in oxygenated aqueous solution. Product identification was by NMR spectroscopy. In products containing double base lesions, guanine is converted to 8-hydroxyguanine and the pyrimidine base is degraded to a formamido remnant.
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Biophysics Department, Roswell Park Cancer Institute, Buffalo, New York 14263.
Freund
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Budzinski
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Wallace
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Maccubbin
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D002851:Chromatography, High Pressure Liquid; D003845:Deoxycytosine Nucleotides / Q000737:chemistry / Q000528:radiation effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects; D015226:Dinucleoside Phosphates / Q000737:chemistry / Q000528:radiation effects*; D005609:Free Radicals; D006151:Guanosine / Q000031:analogs & derivatives; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation; D015394:Molecular Structure; D014965:X-Rays
D003845:Deoxycytosine Nucleotides; D015226:Dinucleoside Phosphates; D005609:Free Radicals; D006151:Guanosine; C037781:deoxycytidylyl-(3'-5')-deoxyguanosine; C046215:8-hydroxyguanosine; C077117:thymidylyl-(3',5')-2'-deoxyguanosine; D003849:Deoxyguanosine