Unexpectedly higher expression levels of a chimeric 2S albumin seed protein transgene from a tandem array construct.
In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expression levels were obtained using a construct in which the transgene was present as a tandem duplication in the T-DNA. Neither in this case nor in homozygous plants with either construct was epigenetic silencing observed. While transgene mRNA levels were of the same order of magnitude as the endogenous at2S2 gene, protein levels were much lower.
Conceição
A da S
Ada S
Plant Genetic Systems N.V., Gent, Belgium.
Van Vliet
A
A
Krebbers
E
E
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008715:Methionine / Q000032:analysis; D009754:Nuts / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012639:Seeds / Q000378:metabolism*; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C036483:T-DNA; D008715:Methionine