Conformational changes in the cyclic undecapeptide cyclosporin induced by interaction with metal ions. An FTIR study.
Infra-red spectra have been measured for the cyclic undecapeptide cyclosporin A (CsA) and three analogues CsC, CsD and CsH in acetonitrile and in the presence of 10:1 molar excess of Mg2+, Ca2+, Na+ or Li+ in the same solvent. Interaction with each of these ions is suggested by marked changes in band positions over the amide I region (1600-1700 cm-1). The formation of complexes of cyclosporin with calcium and magnesium ions is indicated by the presence of C = O stretching bands well outside the range normally expected for the amide I absorptions of free peptides. Although they share this characteristic, the spectra indicate that the mode and/or strength of Ca2+ binding is quite different from that of Mg2+ binding. In contrast, the two monovalent ions interact with CsA, CsC and CsD to yield spectra that are very similar to one another. The spectra are consistent with binding of the monovalent ions simultaneously to several carbonyl groups of the loop structure.
Shaw
R A
RA
Institute for Biodiagnostics, National Research Council of Canada, Winnipeg, Manitoba.
Mantsch
H H
HH
Chowdhry
B Z
BZ
D000097:Acetonitriles; D000595:Amino Acid Sequence; D002118:Calcium / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology*; D003524:Cyclosporins / Q000737:chemistry / Q000187:drug effects*; D008094:Lithium / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012964:Sodium / Q000494:pharmacology; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared
D000097:Acetonitriles; D002412:Cations; D003524:Cyclosporins; D012996:Solutions; D008094:Lithium; D012964:Sodium; D008274:Magnesium; D002118:Calcium; C032159:acetonitrile