title: Genetic studies of lactococcal bacteriophages--taxonomic differentiations and DNA analysis: evidence for 3' cohesive ends.
abstract: Twenty-four bacteriophages of Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris were classified. Two groups of bacteriophages morphologically defined as prolate or isometric types by electron microscopy were examined for their genome sizes, protein patterns and DNA homologies. These criteria showed that prolate phages are quite homogeneous. In contrast, isometric phages exhibit more differences, particularly in particle sizes and protein compositions. Analysis of DNA hybridizations confirmed that prolate phages can be grouped together as can be isometric phages but for one exception, phage I52. These two families were clearly defined. The unique phage which does not fit in either group probably belongs to a third one which is much less represented. No obvious relationships between these criteria and the lytic spectra were detected. Evidence of the presence of cohesive ends in phage genomes is also presented in this study. A more detailed analysis performed on one member of the prolate group revealed 3' protruding ends made up of around 13 nucleotides on complementary single strands.
authors.affiliation: Laboratoire de Genetique Moleculaire, URA CNRS DO256, Université de Rennes I, France.
authors.forename: G
authors.initials: G
authors.lastname: Ermel
authors.forename: A
authors.initials: A
authors.lastname: Cavalier
authors.forename: D
authors.initials: D
authors.lastname: Thomas
authors.forename: J P
authors.initials: JP
authors.lastname: Le Pennec
mesh_terms: D001435:Bacteriophages / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D003611:Dairy Products / Q000382:microbiology; D016679:Genome, Viral; D013294:Lactococcus lactis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000145:classification* / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins