Determination of the concentrations of 5-fluorouracil and its metabolites in rabbit plasma and tissues by high-performance liquid chromatography.
The concentrations of 5-fluorouracil, 5-fluoro-5,6-dihydrouracil, 5-fluorouridine and 5-fluoro-2'-deoxyuridine in plasma, liver, kidney, lung and heart of rabbits were determined by high-performance liquid chromatography (HPLC) after drug administration by two different routes. HPLC was carried out by using a Spherisorb 5 ODS 2 column and 0.05 M phosphate buffer as the mobile phase with UV detection at 200 nm. The pH of the mobile phase, organic modifier content and column temperature were found to have a profound influence on the results, hence it was necessary to optimize a procedure for each matrix. A comparison of the efficiency of intravenous and peritoneal administration revealed that the latter provides higher drug concentrations in the liver and minimal contents in plasma and all other tissues studied.
Del Nozal
M J
MJ
Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain.
Bernal
J L
JL
Pampliega
A
A
Marinero
P
P
Pozuelo
M
M
D000433:1-Propanol; D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004986:Ether; D005472:Fluorouracil / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014018:Tissue Distribution
D007202:Indicators and Reagents; D004986:Ether; D000433:1-Propanol; D005472:Fluorouracil