Species-specificity of monocyte chemotactic protein-1 and -3.
The present study was aimed at defining the activity of human and mouse monocyte chemotactic protein-1 (MCP-1) on homologous and heterologous mononuclear phagocytes. Human natural and recombinant MCP-1 and mouse natural MCP-1/JE were tested as chemoattractants on human blood monocytes and mouse peritoneal macrophages. The human and murine cytokines were equiactive on human monocytes. Human MCP-1 was active on mouse macrophages but the maximal chemotactic effect elicited was about half that of human cells or of mouse MCP-1/JE or of reference chemoattractants. Human MCP-3, a recently identified member of the C-C chemokine family, with high sequence similarity to MCP-1/JE, was also active on mouse mononuclear phagocytes, though less so than mouse MCP-1/JE. These results caution against under-estimating the potential of MCPs when the human chemokines are applied in mice.
Luini
W
W
Istituto di Ricerche Farmacologiche, Milan, Italy.
Sozzani
S
S
Van Damme
J
J
Mantovani
A
A
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D018932:Chemokine CCL2; D054410:Chemokine CCL7; D002630:Chemotactic Factors / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D016207:Cytokines; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018945:Monocyte Chemoattractant Proteins; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013045:Species Specificity
D000890:Anti-Infective Agents; C515450:CCL7 protein, human; C515451:Ccl7 protein, mouse; D018932:Chemokine CCL2; D054410:Chemokine CCL7; D002630:Chemotactic Factors; D016207:Cytokines; D018945:Monocyte Chemoattractant Proteins; D011994:Recombinant Proteins