title: Molecular genetics of chimpanzee Rh-related genes: their relationship with the R-C-E-F blood group system, the chimpanzee counterpart of the human rhesus system.
abstract: As the chimpanzee R-C-E-F blood group system appears to be the chimpanzee counterpart of the human Rhesus (RH) system, we have tried to determine whether chimpanzee Rh-like genes encode R-C-E-F-related proteins. Chimpanzee genomic DNA, digested by any of eight endonucleases and hybridized with three Rh exon-specific probes, exhibits a high degree of polymorphism. Analysis of DNA from unrelated individuals of different R-C-E-F types revealed that the presence of some restriction fragments is correlated with particular R-C-E-F types. The cosegregation of these fragments with R-C-E-F haplotypes was confirmed by family studies. Oligonucleotides complementary to regions flanking human exons were used as PCR primers on chimpanzee DNA; the resulting amplified fragments were identical in size to their human counterparts. Moreover, the nucleotide sequences of the fragments present a high degree of similarity to the corresponding human regions.
authors.affiliation: Centre Régional de Transfusion Sanguine (CRTS), CHU Purpan, Toulouse, France.
authors.forename: I
authors.initials: I
authors.lastname: Salvignol
authors.forename: A
authors.initials: A
authors.lastname: Blancher
authors.forename: P
authors.initials: P
authors.lastname: Calvas
authors.forename: J
authors.initials: J
authors.lastname: Clayton
authors.forename: W W
authors.initials: WW
authors.lastname: Socha
authors.forename: Y
authors.initials: Y
authors.lastname: Colin
authors.forename: J
authors.initials: J
authors.lastname: Ruffié
mesh_terms: D000818:Animals; D001483:Base Sequence; D001789:Blood Group Antigens / Q000235:genetics*; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000235:genetics*; D010375:Pedigree; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001789:Blood Group Antigens