Cloning and characterization of the European seabass, Dicentrarchus labrax, mitochondrial genome.
Mitochondrial DNA (mtDNA) from the European seabass, Dicentrarchus labrax, has been cloned and characterized. Its gene organization was deduced by a comparison of the sequenced termini of different subclones obtained from European seabass mtDNA to the completely-sequenced mtDNAs from carp and freshwater loach. The difference in genome size between the European seabass mtDNA (approximately 18 kb) and most of the other characterized fish mtDNAs (approximately 16.5 kb) is accounted for by the displacement-loop (D-loop). Comparisons have been performed between the derived amino-acid sequences of three sequenced genes, cytochrome c oxidase subunit 2 (COII), NADH dehydrogenase subunit 4L (ND4L) and ATP synthase subunit 8 (ATPase8), from D. labrax, and their counterparts in other fishes and Xenopus laevis.
Venanzetti
F
F
Dipartimento di Biologia, Università degli Studi di Roma Tor Vergata, Italy.
Cecconi
F
F
Giorgi
M
M
Cesaroni
D
D
Sbordoni
V
V
Mariottini
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics*; D002347:Carps / Q000235:genetics; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005399:Fishes / Q000235:genetics; D016678:Genome; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D010802:Phylogeny; D006180:Proton-Translocating ATPases / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014982:Xenopus laevis / Q000235:genetics
D004272:DNA, Mitochondrial; D046911:Macromolecular Substances; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D006180:Proton-Translocating ATPases