Interferon-gamma production of mitogen stimulated peripheral lymphocytes in perinatal cows.
The production of interferon-gamma (IFN-gamma) in bovine peripheral blood mononuclear cells (PBMC) during the perinatal period was studied. IFN-gamma activity was neutralized with monoclonal antibodies (MoAb) to recombinant bovine IFN-gamma. IFN-gamma production was apparently suppressed by treatment of the PBMC with MoAb to bovine pan-T cell. IFN-gamma was more susceptible to treatment with MoAb to CD4+ than that with MoAb to bovine CD8+, suggesting that the CD4+ T-cell subset was the major producer of the lymphokine formed in the culture. IFN-gamma production by concanavalin A (Con A) stimulation was found to be significantly depressed throughout the perinatal period (from 5 weeks before to 7 weeks after parturition) when compared to that of the non-pregnant control group, but phytohemagglutinin (PHA)-induced IFN-gamma production was markedly depressed from 3 weeks before to 2 weeks after calving. The addition of recombinant human interleukin 2 (100 IU/ml) to the PBMC cultures from perinatal cows resulted in the augmentation of IFN-gamma production by stimulation with Con A or PHA.
Ishikawa
H
H
Veterinary Hospital, Hokkaido, Japan.
Shirahata
T
T
Hasegawa
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002417:Cattle / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000037:antagonists & inhibitors; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology*; D009500:Neutralization Tests; D010835:Phytohemagglutinins / Q000494:pharmacology; D049590:Postpartum Period; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D007370:Interferon Type I; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011994:Recombinant Proteins; D003208:Concanavalin A; D007371:Interferon-gamma