title: Malassezia furfur-related colonization and infection of central venous catheters. A prospective study in a pediatric intensive care unit.
abstract: OBJECTIVE
  To determine the incidence of Malassezia furfur-related colonization and infection of central venous catheters.
  
  
  METHODS
  Prospective clinical study.
  
  
  METHODS
  A paediatric intensive care unit at a University Hospital.
  
  
  METHODS
  66 newborns with central venous catheters for parenteral nutrition including lipid emulsions (Intralipid).
  
  
  METHODS
  When a central venous catheter was removed, it was rinsed with 1 ml of physiological saline, transported at ambient temperature to the clinical laboratory and cultured on Dixon's medium. The tip of the central venous catheter was used for a bacteriological study using Maki's technique. In case of suspected sepsis, blood cultures were obtained using an Isolator tube. RESULTS. 74 central venous catheters were included: mean duration of use of a central venous catheters and infusions of lipid emulsion (Intralipid) were 19.3 +/- 10 days and 8.6 +/- 8 days respectively. Only 2 central venous catheters (2.7%) were colonized by Malassezia furfur: (Mf) one in an asymptomatic newborn, and the other in an infected newborn with signs of sepsis, who most probably died at 4 months of age from refractory hypoxia due to pulmonary hypoplasia, but not from Mf sepsis.
  
  
  CONCLUSIONS
  The incidence of Malassezia furfur-related colonization of central venous catheters appears to be low but not negligible, which warrants the use of specific culture techniques.
authors.affiliation: Pediatric Intensive Care and Neonatology Department, Centre Hospitalier Universitaire, Brest, France.
authors.forename: J
authors.initials: J
authors.lastname: Sizun
authors.forename: A
authors.initials: A
authors.lastname: Karangwa
authors.forename: J D
authors.initials: JD
authors.lastname: Giroux
authors.forename: O
authors.initials: O
authors.lastname: Masure
authors.forename: A M
authors.initials: AM
authors.lastname: Simitzis
authors.forename: D
authors.initials: D
authors.lastname: Alix
authors.forename: L
authors.initials: L
authors.lastname: De Parscau
mesh_terms: D001431:Bacteriological Techniques; D002405:Catheterization, Central Venous; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D008290:Malassezia / Q000254:growth & development / Q000302:isolation & purification*; D011446:Prospective Studies; D018805:Sepsis / Q000382:microbiology*