Use of enzyme-linked immunosorbent assay (ELISA) for detection and quantification of monoclonal antibodies.
The thrust of the present work is the demonstration of the feasibility of using an enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of a monoclonal antibody human serum. The antibodies used, 7E3 and D3GP3, have been proposed as platelet receptor blockers, being directed specifically against the platelet membrane receptors (glycoprotein IIb/IIIa) and thus of significance in the management of patients at a very hgih risk of thrombotic occlusion. It is shown that 7E3 is more easily adsorbed than D3GP3 and hence has a higher potential for the stated application.
Lewis
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Department of Mechanical Engineering, University of Memphis, TN 38152.
D000911:Antibodies, Monoclonal / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005240:Feasibility Studies; D005343:Fibrinolytic Agents; D006801:Humans; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D016024:Receptors, Cytoadhesin / Q000276:immunology*; D013053:Spectrophotometry; D013927:Thrombosis / Q000097:blood / Q000276:immunology
D000911:Antibodies, Monoclonal; D005343:Fibrinolytic Agents; D010980:Platelet Membrane Glycoproteins; D016024:Receptors, Cytoadhesin