Mouse macrophage derived monocyte chemotactic protein-3: cDNA cloning and identification as MARC/FIC.
When the mouse macrophage cell line WEHI-3 is triggered with LPS it produces proteases and secondary cytokines including interleukin-6 and chemokines. In an attempt to isolate the mouse homologue of the human monocyte chemotactic protein-3 (MCP-3), a cDNA library from LPS-stimulated WEHI-3 cells was screened with the full-size human MCP-3 cDNA. The longest cDNA out of several positive clones was sequenced and encoded a protein of 97 residues. Except for a third codon letter mismatch it was identical to the mouse MARC cDNA and encoded the MARC protein. The murine Fic cDNA, which encodes a Marc-mutant protein with an arginine substitution for alanine, was not identified in the other sequenced homologous isolates. Similar to the human system, in which MCP-3 is most related to MCP-1, MURINE MCP-3 was found to be more homologous to mouse MCP-1/JE than to other murine C-C chemokines. We therefore postulate that MARC/FIC is the mouse MCP-3.
Thirion
S
S
Rega Institute for Medical Research, University of Leuven, Belgium.
Nys
G
G
Fiten
P
P
Masure
S
S
Van Damme
J
J
Opdenakker
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018932:Chemokine CCL2; D054410:Chemokine CCL7; D002630:Chemotactic Factors / Q000096:biosynthesis* / Q000737:chemistry; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis* / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000737:chemistry; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D018945:Monocyte Chemoattractant Proteins; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
C515450:CCL7 protein, human; C515451:Ccl7 protein, mouse; D018932:Chemokine CCL2; D054410:Chemokine CCL7; D002630:Chemotactic Factors; D016207:Cytokines; D018076:DNA, Complementary; D017874:Immediate-Early Proteins; D018945:Monocyte Chemoattractant Proteins; D004247:DNA