title: Characterization of the aac(6')-Ik gene of Acinetobacter sp. 6.
abstract: The aac(6')-Ik gene which confers resistance to aminoglycosides in Acinetobacter sp. 6 CIP A165 was characterized. The resistance gene was identified as a coding sequence of 438 bases pairs corresponding to a protein with a calculated mass of 16627 Da. Alignment of aac(6')-Ik with aac(6')-Ig from Acinetobacter haemolyticus indicated 83% identity. Like aac(6')-Ig of A. haemolyticus and aac(6')-Ij of Acinetobacter sp. 13, the aac(6')-Ik gene was apparently located in the chromosome and was species specific. The high degree of identity between aac(6')-Ig and -Ik, compared with genomic DNA relationships between the host species, indicated that these genes have diverged from a common ancestor in a parental Acinetobacter species.
authors.affiliation: Centre d'Etudes Pharmaceutiques, Chatenay-Malabry, France.
authors.forename: E
authors.initials: E
authors.lastname: Rudant
authors.forename: P
authors.initials: P
authors.lastname: Bourlioux
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
authors.forename: T
authors.initials: T
authors.lastname: Lambert
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000150:Acinetobacter / Q000187:drug effects / Q000235:genetics*; D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000617:Aminoglycosides; D000123:Acetyltransferases; C056532:aminoglycoside N(6')-acetyltransferase