title: In vivo observation of the human tear film by tandem scanning confocal microscopy.
abstract: A new method for observing normal and pathologic states of the human tear film using tandem scanning confocal microscopy is presented. The confocal microscope is configured with a horizontal light path, a 10 x dry objective, and an image-intensified camera for collecting images at a magnification of approximately 150x. The advantages of confocal microscopy can be used to collect reflected images of the human tear film with improved detail and resolution.
authors.affiliation: Department of Ophthalmology, University of Iowa Hospitals and Clinics, Iowa City 52242-1091.
authors.forename: W D
authors.initials: WD
authors.lastname: Mathers
authors.forename: T E
authors.initials: TE
authors.lastname: Daley
mesh_terms: D006801:Humans; D008055:Lipids / Q000032:analysis; D018613:Microscopy, Confocal / Q000379:methods; D013666:Tears / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008055:Lipids