title: Enzymically produced cyclic alpha-1,3-linked and alpha-1,6-linked oligosaccharides of D-glucose.
abstract: A new type of bacterial enzyme hydrolyzed alternan (Leuconostoc mesenteroides NRRL B-1355 fraction S dextran, an alternating alpha-1,3-alpha-1,6-D-glucan) to give rise to a series of oligosaccharides. The oligosaccharide formed in the greatest proportion was a cyclic tetrasaccharide of D-glucosyl residues linked in an alternating alpha-1,3-alpha-1,6 fashion. Other saccharide products included isomaltose and alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,6-D-glucose. Oligosaccharides of higher degrees of polymerization were also formed, and included alpha-D-glucosylated derivatives of the cyclic tetrasaccharide. This is the first report of a naturally produced cyclic tetrasaccharide.
authors.affiliation: National Center for Agricultural Research, United States Department of Agriculture, Illinois.
authors.forename: G L
authors.initials: GL
authors.lastname: Côté
authors.forename: P
authors.initials: P
authors.lastname: Biely
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D003500:Cyclization; D004187:Disaccharides / Q000032:analysis; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D007534:Isomaltose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*
chemical_list: D004187:Disaccharides; D009844:Oligosaccharides; C014637:laminaribiose; D007534:Isomaltose; D006026:Glycoside Hydrolases; C091143:alternanase; D005947:Glucose