Secretion of plasminogen activator inhibitor type 1 by cultured ovarian cells obtained from gonadotropin-treated immature rats.
It is demonstrated that i) theca-interstitial compartment synthesizes the majority of plasminogen activator inhibitor type 1 (PAI-1) in the ovary before ovulation, and the follicular wall may therefore serve as a specific barrier with the presence of PAI-1 activity to prevent the secretion of tPA into the extrafollicular compartments; ii) granulosa cells secrete only a small amount of ovarian PAI-1, but synthesize the most of tissue-type plasminogen activator tPA involved in the processes leading to ovulation; iii) since only matured cumulus-oocyte complexes secrete a large amount of tPA and PAI-1, both tPA and PAI-1 activity in the conditioned medium may be used as reliable markers for evaluating oocyte quality for in vitro fertilization.
Liu
Y X
YX
Laboratory of Reproductive Biology, Institute of Zoology, Academia Sinica, Beijing, PRC.
Feng
Q
Q
Peng
X R
XR
Tor
N
N
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006062:Gonadotropins / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D010060:Ovulation / Q000187:drug effects; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013799:Theca Cells / Q000187:drug effects / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis
D006062:Gonadotropins; D017395:Plasminogen Activator Inhibitor 1; D010959:Tissue Plasminogen Activator