A semi-preparative enzymic synthesis of malonyl-CoA from [14C]acetate and 14CO2: labelling in the 1, 2 or 3 position.
A semi-preparative enzymic synthesis of [1-14C]malonyl-CoA from [1-14C]acetate and bicarbonate, and of [3-14C]malonyl-CoA from Na2(14)CO3 and acetate, was achieved by using chloroplasts rapidly isolated from 7-8-day-old pea shoots. Around 70% of the [1-14C]acetate was converted into malonyl-CoA in 2-3 h, and the specific radioactivity of [3-14C]malonyl-CoA synthesized in the system was 25-30 Ci/mol. Reactions were monitored and labelled products were purified by h.p.l.c.
Roughan
G
G
Horticulture and Food Research Institute of New Zealand Ltd., Mt. Albert Research Centre, Auckland.
D000085:Acetates / Q000737:chemistry*; D002245:Carbon Dioxide / Q000737:chemistry*; D002250:Carbon Radioisotopes; D002736:Chloroplasts / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry; D007700:Kinetics; D008316:Malonyl Coenzyme A / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010946:Plants, Medicinal
D000085:Acetates; D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D008316:Malonyl Coenzyme A; D010744:Phosphoric Monoester Hydrolases; D010450:Endopeptidases