Human carcinoma cell lines produce biologically active leukemia inhibitory factor (LIF).
We investigated the production of leukemia inhibitory factor (LIF) by human carcinoma cell lines. LIF mRNA was detected by Northern blot analysis in all 24 carcinoma cell lines of the lung, breast, stomach, colon, liver, gallbladder, pancreas and melanocytes. Seventeen of them (70.8%) secreted LIF in the culture supernatant (range: 40.4-3990.3 pg/ml, mean +/- SEM: 611.8 +/- 262.9 pg/ml). Biologic activity of LIF was confirmed in the culture supernatant of carcinoma cell lines by the MTT assay using M1 cells. The present results showed that human carcinoma cell lines are constitutively producing biologically active LIF. The possible biological significance of LIF produced by cancer cells is discussed.
Kamohara
H
H
Department of Surgery II, Kumamoto University Medical School, Japan.
Sakamoto
K
K
Ishiko
T
T
Mita
S
S
Masuda
Y
Y
Abe
T
T
Ogawa
M
M
D015152:Blotting, Northern; D006131:Growth Inhibitors / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008175:Lung Neoplasms / Q000378:metabolism; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D009369:Neoplasms / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000378:metabolism; D014407:Tumor Cells, Cultured
D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D012333:RNA, Messenger; D011994:Recombinant Proteins