title: Isoflurane inhibits hypoxic pulmonary vasoconstriction. An in vivo fluorescence microscopic study in rabbits.
abstract: BACKGROUND
  Contradictory results have been reported in previous studies investigating the effect of isoflurane on hypoxic pulmonary vasoconstriction by indirect approaches. The current study measured the effects of one-lung ventilation (1LV) and isoflurane 1.5% by direct visual observation of the pulmonary microcirculation.
  
  
  METHODS
  Ten New Zealand White rabbits were anesthetized with intravenous thiopental, alpha-chloralose, and piritramid. Arterial, central venous, pulmonary arterial, left atrial, and airway pressures and cardiac output were recorded continuously. 1LV was facilitated by a bronchial blocker in the right main bronchus. A transparent window was implanted into the right thoracic wall for videofluorescence microscopy of the subpleural pulmonary microcirculation. After intravenous injection of fluorescein isothiocyanate-labeled red blood cells, vessel diameters, red blood cell flux, red blood cell velocity, and dynamic microhematocrit were measured in pulmonary arterioles and venules during two-lung ventilation and 1LV during baseline anesthesia and with supplementary isoflurane 1.5%.
  
  
  RESULTS
  During intravenous anesthesia, 1LV caused significant reduction of vessel diameters and red cell flux and velocity and an increase in microvascular hematocrit in pulmonary arterioles and venules. The decreases in arteriolar diameters and red blood cell flux and velocity induced by 1LV were significantly attenuated by isoflurane as compared with those measured during baseline anesthesia (P = 0.010, P = 0.029 and P = 0.047). Accordingly, 1LV-induced reduction of venular red cell flux (P = 0.023) and velocity (P = 0.036) were less pronounced during isoflurane. Isoflurane caused a significant decrease in arterial pressure. Venous admixture increased and arterial oxygen tension decreased significantly during 1LV; the changes were more pronounced during 1LV with isoflurane 1.5% than during 1LV with baseline anesthesia.
  
  
  CONCLUSIONS
  1LV leads to a marked reduction of microvascular diameters and blood flow in the hypoxic lung. Isoflurane 1.5% inhibits hypoxic pulmonary vasoconstriction in pulmonary arterioles and increases regional blood flow in the hypoxic lung.
authors.affiliation: Institute of Anesthesiology, Ludwig-Maximilians-University, Klinikum Grosshadern, Munich.
authors.forename: J
authors.initials: J
authors.lastname: Groh
authors.forename: G E
authors.initials: GE
authors.lastname: Kuhnle
authors.forename: A
authors.initials: A
authors.lastname: Sckell
authors.forename: L
authors.initials: L
authors.lastname: Ney
authors.forename: A E
authors.initials: AE
authors.lastname: Goetz
mesh_terms: D000771:Anesthesia, Intravenous / Q000009:adverse effects*; D000818:Animals; D006439:Hemodynamics / Q000187:drug effects; D000860:Hypoxia / Q000188:drug therapy* / Q000209:etiology; D007530:Isoflurane / Q000627:therapeutic use*; D008168:Lung / Q000187:drug effects*; D008856:Microscopy, Fluorescence; D011652:Pulmonary Circulation / Q000187:drug effects; D011659:Pulmonary Gas Exchange; D011817:Rabbits; D013874:Thiopental; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: D007530:Isoflurane; D013874:Thiopental