Activation of plant plasma membrane H(+)-ATPase by the non-ionic detergent Brij 58.
Plasma membrane vesicles were purified from tobacco callii and the modulation of H(+)-ATPase by detergents was investigated. The nonionic detergent Brij 58 not only activated ATP hydrolysis (2-fold) but also proton pumping (more than 4-fold). Triton X-100, within a more limited concentration range, produced a similar effect. The simultaneous activation of ATP hydrolysis and proton pumping is not compatible with current interpretations of effects of nonionic detergents on the H(+)-ATPase based on latency of the enzyme and opening of vesicles.
Ibarz
E
E
European Molecular Biology Laboratory, Heidelberg, Germany.
Palmgren
M G
MG
Palazón
J
J
Piñol
M T
MT
Serrano
R
R
D002462:Cell Membrane / Q000201:enzymology; D002592:Cetomacrogol / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D010947:Plants, Toxic; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology*
D002592:Cetomacrogol; D006180:Proton-Translocating ATPases