Cytomegalovirus hepatitis: characterization of the inflammatory infiltrate in resistant and susceptible mice.
Mice susceptible and resistant to murine cytomegalovirus (MCMV) were infected with this virus and livers were harvested after 2-231 days. Cryostat sections were stained to visualize cells bearing CD4, CD8 or Mac-1 antigens. Mac-1+ cells were prevalent in inflammatory foci after 2 days. These cells persisted in susceptible BALB/c and A/J mice, but disappeared from livers of resistant C57Bl/6 and CBA/CaH mice by day 28. T cell inflammation peaked on days 7-11. This declined by day 56 in C57Bl/6 and CBA/CaH mice, but persisted in BALB/c and A/J mice for at least 231 days. Persistent CD8+ cells were dispersed throughout the parenchyma. More CD8+ cells were observed 7-14 days after infection in the livers of bg/bg (natural killer (NK) cell-deficient) C57Bl/6 and CBA mice, and in C57Bl/6 mice depleted of NK1.1 cells by MoAb. Thus, mice of strains susceptible to MCMV exhibit hepatitis characterized by persistence of dispersed CD8+ cells. This phenomenon may be limited by NK cells in resistant strains.
Olver
S D
SD
Department of Microbiology, University of Western Australia, Nedlands.
Price
P
P
Shellam
G R
GR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005260:Female; D006098:Granulocytes / Q000276:immunology; D006524:Hepatitis, Viral, Animal / Q000276:immunology* / Q000473:pathology / Q000821:virology*; D006566:Herpesviridae Infections; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology; D008099:Liver / Q000473:pathology* / Q000821:virology*; D051379:Mice; D008815:Mice, Inbred Strains; D018146:Muromegalovirus; D010641:Phenotype; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000911:Antibodies, Monoclonal