5-Hydroxytryptamine2 and 5-hydroxytryptamine3 receptors mediate serotonin-induced short-circuit current in pig jejunum.
The purpose of this study was to determine the effect of methysergide, ketanserin, granisetron, cisapride, and renzapride on serotonin (5-hydroxytryptamine-evoked short-circuit current in muscle and myenteric plexus-stripped pig jejunum using the Ussing chamber technique. Ketanserin, granisetron, cisapride, and renzapride all reduced the 5-hydroxytryptamine-induced increase in short-circuit current by about 50%. Combination of ketanserin and granisetron only reduced the 5-hydroxytryptamine-induced peak increase in short-circuit current by 25%. Cisapride caused a small concentration-dependent increase in short-circuit current. Atropine and hexamethonium both almost completely suppressed the cisapride-induced peak increase in short-circuit current. Atropine and hexamethonium both almost completely suppressed the cisapride-induced peak increase in short-circuit current. Ketanserin, granisetron, methysergide, and renzapride did not alter the basal short-circuit current. These results suggest that 5-hydroxytryptamine elicits an increase in short-circuit current by activating epithelial and submucosal 5-hydroxytryptamine2 and 5-hydroxytryptamine3 receptor subtypes. Furthermore, the short-circuit current-increasing effect of cisapride, is due to activation of at least muscarinic and nicotinic receptors.
Hansen
M B
MB
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Thorbøll
J E
JE
Skadhauge
E
E
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D004594:Electrophysiology; D005260:Female; D017829:Granisetron / Q000494:pharmacology; D007583:Jejunum / Q000187:drug effects* / Q000502:physiology*; D007650:Ketanserin / Q000494:pharmacology; D011985:Receptors, Serotonin / Q000145:classification* / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology*; D013552:Swine
D001549:Benzamides; D017829:Granisetron; D007650:Ketanserin; D011985:Receptors, Serotonin; D012701:Serotonin