Gradient elution in micellar liquid chromatography. II. Organic modifier gradients.
The application of organic modifier gradients in micellar liquid chromatography (MLC) is discussed. The equation derived by Snyder and co-workers describing gradient elution in hydro-organic reversed-phase LC was verified for organic solvent gradients in the presence of micelles. It is also demonstrated that the use of these gradients require little re-equilibration time due to the limited range of organic modifier concentration used in the gradient. This would result in shorter analysis time. Lastly, a practical application of the use of propanol and acetonitrile gradients in MLC is described.
Madamba-Tan
L S
LS
Department of Chemistry, North Carolina State University, Raleigh 27695-8204.
Strasters
J K
JK
Khaledi
M G
MG
D002853:Chromatography, Liquid / Q000379:methods*; D007202:Indicators and Reagents; D008823:Micelles; D012997:Solvents
D007202:Indicators and Reagents; D008823:Micelles; D012997:Solvents