Prostaglandin E2/parathyroid hormone-induced suppression of alkaline phosphatase activity is mediated by protein kinase C.
1. Bone resorptive factors, prostaglandin E2 and parathyroid hormone are shown to suppress alkaline phosphatase activity in a rat osteoblastic cell line. 2. Phorbol myristate acetate, but not dibutyryl cAMP or calcium ionophore can suppress alkaline phosphatase activity. 3. The protein kinase C inhibitors (H89, staurosporine) are able to block the suppression of alkaline phosphatase activity induced by prostaglandin E2 and parathyroid hormone. 4. These data suggest that protein kinase C is involved in the inhibition of alkaline phosphatase activity induced by prostaglandin E2 and parathyroid hormone.
Miya
T
T
Department of Oral Surgery, School of Medicine, Chiba University, Japan.
Tagawa
M
M
Kato
N
N
Takahashi
K
K
Sato
K
K
Fujimura
S
S
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D003513:Cycloheximide / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology*; D007546:Isoquinolines / Q000494:pharmacology; D010006:Osteoblasts / Q000201:enzymology*; D012516:Osteosarcoma; D010281:Parathyroid Hormone / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D019311:Staurosporine; D013449:Sulfonamides; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D007546:Isoquinolines; D010281:Parathyroid Hormone; D013449:Sulfonamides; D000001:Calcimycin; D003994:Bucladesine; D003513:Cycloheximide; D000242:Cyclic AMP; D011493:Protein Kinase C; D000469:Alkaline Phosphatase; D019311:Staurosporine; D015232:Dinoprostone; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; D013755:Tetradecanoylphorbol Acetate