[The use of ELISA and indirect immunofluorescence technics for the rapid detection of eastern equine encephalomyelitis].
We present the results attained in the identification of Eastern equine encephalomyelitis virus isolations in Vero and XL-2 cell systems, using a double-antibody ELISA technique and the indirect immunofluorescence method. The results attained through these two techniques coincided by 100% with identification through neutralization. With the former, the virus was detected within 6-8 hours after inoculation. Better results were attained with XL-2 cells.
Pelegrino
J L
JL
Instituto de Medicina Tropical Pedro Kourí.
Vázquez
S
S
Morier
L
L
Castillo
A
A
Guzmán
M G
MG
Kourí
G
G
D000818:Animals; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004663:Encephalitis Virus, Eastern Equine / Q000276:immunology* / Q000302:isolation & purification; D004683:Encephalomyelitis, Equine / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D009500:Neutralization Tests / Q000662:veterinary; D013997:Time Factors
Utilización de las técnicas ELISA e inmunofluorescencia indirecta para la detección rápida de la encefalomielitis equina del este.