title: Replication of human cytomegalovirus in a rhabdomyosarcoma cell line depends on the state of differentiation of the cells.
abstract: The replication of human cytomegalovirus (HCMV) was investigated in a new human rhabdomyosarcoma cell line (KFR) with morphological and biochemical characteristics of fetal striated muscle precursors (rhabdomyoblasts). KFR cells exhibited the unique property for spontaneous morphological transformation from a poorly-differentiated state into well-differentiated (myotube-like) rhabdomyoblasts. The poorly-differentiated rhabdomyoblasts promoted both complete viral gene expression and the production of infectious virus. In contrast, in well-differentiated rhabdomyoblasts HCMV infection was abortive. The results showed that replication of HCMV in this human rhabdomyosarcoma cell line depended on the state of cellular differentiation.
authors.affiliation: Centre of Hygiene, Institute of Medical Virology, J. W. Goethe University, Frankfurt a. M, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: K
authors.initials: K
authors.lastname: Radsak
authors.forename: H
authors.initials: H
authors.lastname: Rabenau
authors.forename: B
authors.initials: B
authors.lastname: Weber
authors.forename: M
authors.initials: M
authors.lastname: Novak
authors.forename: R
authors.initials: R
authors.lastname: Benda
authors.forename: B
authors.initials: B
authors.lastname: Kornhuber
authors.forename: H W
authors.initials: HW
authors.lastname: Doerr
mesh_terms: D000956:Antigens, Viral / Q000096:biosynthesis; D001483:Base Sequence; D002454:Cell Differentiation; D003587:Cytomegalovirus / Q000276:immunology / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology / Q000821:virology*; D012208:Rhabdomyosarcoma / Q000473:pathology; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D014764:Viral Proteins; C044582:immediate-early proteins, cytomegalovirus