Detection of Epstein-Barr virus small RNAs in routine paraffin sections using non-isotopic RNA/RNA in situ hybridization.
The Epstein-Barr virus (EBV) is associated with an increasing range of reactive and neoplastic lesions. There is a need for a sensitive and specific method for detecting latent EBV in routine histological sections. We report the use of a highly sensitive paraffin section RNA/RNA in situ hybridization (ISH) technique using digoxigenin-labelled antisense riboprobes for demonstrating EBV encoded small RNAs (EBERs), EBV gene products that are transcribed in abundance during latent EBV infection. We applied EBER-ISH to 846 paraffin embedded specimens, including cases of reactive lymphoid hyperplasia (n = 28), infectious mononucleosis (16), Burkitt's lymphoma (44), immunodeficiency-associated lymphomas in transplant recipients (9) and AIDS patients (128), Hodgkin's disease (130), CD30 antigen positive lymphomas (106), peripheral T-cell lymphomas (104), sporadic B-cell non-Hodgkin's lymphomas (162), undifferentiated nasopharyngeal carcinoma (86), salivary gland lymphoepithelioma (11), and oral hairy leukoplakia (5). Strong, reproducible EBER staining was seen in EBV latently infected cells in archival surgical biopsy and autopsy specimens. EBER-ISH is specific, has a sensitivity comparable to that of the polymerase chain reaction, and is now the method of choice for the in situ detection of latent EBV infection.
Hamilton-Dutoit
S J
SJ
Laboratory of Immunopathology, Aarhus University Hospital, Denmark.
Pallesen
G
G
D000818:Animals; D002277:Carcinoma / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000821:virology; D006566:Herpesviridae Infections / Q000175:diagnosis; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008223:Lymphoma / Q000821:virology; D051379:Mice; D016513:Mice, SCID; D009303:Nasopharyngeal Neoplasms / Q000821:virology; D016612:Paraffin Embedding; D012367:RNA, Viral / Q000302:isolation & purification*; D012468:Salivary Gland Neoplasms / Q000821:virology; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000175:diagnosis
D012367:RNA, Viral