title: Lipid peroxidation and antioxidant enzyme activities in the rat testis after cigarette smoke inhalation or administration of polychlorinated biphenyls or polychlorinated naphthalenes.
abstract: Lipid peroxidation products and antioxidant enzyme activities were studied in the rat testis following exposures to cigarette smoke, polychlorinated biphenyls (PCBs), or polychlorinated naphthalenes (PCNs). Three hours after a single 1-hour period of smoke inhalation, the levels of fluorescent chromolipids and thiobarbituric acid-reactive species (TBARS) were markedly increased in the testis (+49%, P < 0.01, and +43%, P < 0.05, respectively). Twelve hours after daily smoking for 1 hour, for 1, 5, or 10 days, such an increase was not found. Activities of the antioxidant enzymes superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px), glutathione transferase (GSH-Tr), or hexose monophosphate shunt (HMS) were not affected immediately, 3 hours, or 12 hours after a single smoking session. Twelve hours after smoking for 5 days, the activity of catalase was decreased (-16%, P < 0.05). Smoking exposures had no consistent effects on serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), or testosterone concentrations. Single i.p. injections of PCB or PCN mixtures resulted in decreases in testicular SOD activity 1 day after the exposures (-14%, P < 0.05, and -51%, P < 0.01, respectively). Catalase activity also decreased after both exposures (-30 to -42%, P < 0.05, at days 1-7 after PCB exposure, and -37 to -43%, P < 0.05, at days 3-7 after PCN exposure). Ninety days after the PCN exposure, activities of GSH-Px and GSH-Tr were decreased in the testis (-20%, P < 0.05, and -26%, P < 0.05, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology, University of Turku, Finland.
authors.forename: V
authors.initials: V
authors.lastname: Peltola
authors.forename: E
authors.initials: E
authors.lastname: Mäntylä
authors.forename: I
authors.initials: I
authors.lastname: Huhtaniemi
authors.forename: M
authors.initials: M
authors.lastname: Ahotupa
mesh_terms: D000818:Animals; D002374:Catalase / Q000032:analysis* / Q000502:physiology; D005453:Fluorescence; D005640:Follicle Stimulating Hormone / Q000097:blood; D005979:Glutathione Peroxidase / Q000032:analysis / Q000502:physiology; D015227:Lipid Peroxidation / Q000502:physiology*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009281:Naphthalenes / Q000008:administration & dosage / Q000494:pharmacology*; D010427:Pentose Phosphate Pathway / Q000502:physiology; D011078:Polychlorinated Biphenyls / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012907:Smoking; D013482:Superoxide Dismutase / Q000032:analysis* / Q000502:physiology; D013737:Testis / Q000201:enzymology* / Q000378:metabolism*; D013739:Testosterone / Q000097:blood; D013997:Time Factors
chemical_list: D009281:Naphthalenes; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D011078:Polychlorinated Biphenyls; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase