Stable-isotope dilution GC-MS for determination of toluene in submilliliter volumes of whole blood.
An accurate and sensitive yet simple protocol for the analysis of toluene in submilliliter quantities of whole blood using stable-isotope dilution GC-MS has been developed and evaluated for use in pharmacokinetic studies of toluene exposure. The method involves the use of toluene-d8, a relatively inexpensive chemical used as a solvent for nuclear magnetic resonance spectroscopy, as an internal standard and extraction with pentane. This procedure allows for the determination of toluene in whole blood down to approximately 5 ng/mL and is suitable for pharmacokinetic measurements and biomonitoring of exposure to toluene.
Jones
A D
AD
Facility for Advanced Instrumentation, University of California, Davis 95616.
Dunlap
M R
MR
Gospe
S M
SM
D000818:Animals; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007201:Indicator Dilution Techniques; D007554:Isotopes; D008297:Male; D011247:Pregnancy; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D014050:Toluene / Q000097:blood*
D007554:Isotopes; D014050:Toluene