Effect of mitomycin C, verapamil, and hyperthermia on human gastric adenocarcinoma.
The purpose of this study was to assess the efficacy of verapamil (20 microM) and hyperthermia (42 degrees C) as modifiers of mitomycin C (MMC), used at different concentrations, in inhibiting the growth of human gastric adenocarcinoma (AGS) cells. Combined verapamil and hyperthermia treatment resulted in a significant decrease in cell count by 72.2% as compared with the control value. Verapamil drastically enhanced the growth-inhibitory activity of MMC at high concentration against AGS cells by 67.5% and had no effect at intermediate and low concentrations. Hyperthermia did not enhance the effect of MMC on AGS cells. The modalities analyzed in this study require further investigation and may have potential for in vivo studies on gastric cancer therapy in the near future.
Shchepotin
I B
IB
Department of Surgery, Georgetown University, Washington, D.C.
Buras
R R
RR
Nauta
R J
RJ
Shabahang
M
M
Evans
S R
SR
D000230:Adenocarcinoma / Q000188:drug therapy / Q000628:therapy*; D003131:Combined Modality Therapy; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced; D008875:Middle Aged; D016685:Mitomycin / Q000627:therapeutic use*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000628:therapy*; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000627:therapeutic use*
D016685:Mitomycin; D014700:Verapamil