title: Chimerism in PCR products from a multigene family.
abstract: This paper describes a potential hazard in obtaining sequences of a multigene family by amplifying the mixed population by the polymerase chain reaction with primers from conserved sequences. We describe a product which appears to be a chimera formed during the PCR reaction from two different but related sequences.
authors.affiliation: Department of Biochemistry, Tufts Medical School, Boston, MA 02111.
authors.forename: D
authors.initials: D
authors.lastname: Bhavsar
authors.forename: H
authors.initials: H
authors.lastname: Zheng
authors.forename: J
authors.initials: J
authors.lastname: Drysdale
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D005293:Ferritins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics*
chemical_list: D011993:Recombinant Fusion Proteins; D004247:DNA; D005293:Ferritins