Characterization and in situ localization of a salt-induced tomato peroxidase mRNA.
NaCl treatment of tomato plants in hydroponic culture at concentrations as low as 50 mM resulted in enhanced accumulation of transcripts of TPX1, a full-length cDNA clone that we had isolated from a library of NaCl-treated tomato plants using a peroxidase-specific oligonucleotide probe. Although the overall amino acid sequence identity of TPX1 to other peroxidase genes was less than 45%, there was a very high degree of identity in all of the conserved domains. The deduced amino acid sequence included the presence of a N-terminal signal peptide but not the C-terminal extension present in peroxidases targeted to the vacuole. The mature protein has a theoretical pI value of 7.5. Transcripts that hybridized to TPX1 were detected only in the roots with higher levels of mRNA in epidermal and subepidermal cell layers. Isoelectric focusing of root extracts showed two major bands of peroxidase activity at pI 5.9 and 6.2. Both activities increased with salt treatment. Southern analysis indicated the presence of only a single TPX1 gene in tomato.
Botella
M A
MA
Departamento de Bioquímica y Biología Molecular, Universidad de Málaga, Spain.
Quesada
M A
MA
Kononowicz
A K
AK
Bressan
R A
RA
Pliego
F
F
Hasegawa
P M
PM
Valpuesta
V
V
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000737:chemistry; D004790:Enzyme Induction / Q000187:drug effects; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics*; D010940:Plant Proteins; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology*; D014675:Vegetables / Q000201:enzymology* / Q000235:genetics
D018076:DNA, Complementary; D007527:Isoenzymes; D010940:Plant Proteins; D012333:RNA, Messenger; D012965:Sodium Chloride; D010544:Peroxidases; C087772:TPX1 protein, Lycopersicon esculentum