title: Inhibition of the respiratory burst in mouse macrophages by ultra-low doses of an opioid peptide is consistent with a possible adaptation mechanism.
abstract: The respiratory burst induced by phorbol myristate acetate in mouse macrophages was inhibited by ultra-low doses (10(-15)-10(-13) M) of an opioid peptide [D-Ala2]methionine enkephalinamide. The effect disappeared at concentrations above and below this range. The inhibition approached 50% and was statistically significant (P < 0.001). Increasing the time of the opioid incubation with cells brought about a shift in the maximal effect to lower concentrations of the opioid (from 10(-13) to 5 x 10(-15) M) and led to a decrease in the value of the effect, fully in accord with the previously proposed adaptation mechanism of the action of ultra-low doses.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
authors.forename: A M
authors.initials: AM
authors.lastname: Efanov
authors.forename: A A
authors.initials: AA
authors.lastname: Koshkin
authors.forename: L A
authors.initials: LA
authors.lastname: Sazanov
authors.forename: O I
authors.initials: OI
authors.lastname: Borodulina
authors.forename: S D
authors.initials: SD
authors.lastname: Varfolomeev
authors.forename: S V
authors.initials: SV
authors.lastname: Zaitsev
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004744:Enkephalin, Methionine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D016897:Respiratory Burst / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D004744:Enkephalin, Methionine; C022118:enkephalinamide-Met, Ala(2)-; D013755:Tetradecanoylphorbol Acetate