[Effect of bis(n-tributyltin)-oxide on the brain GABA-ergic system in vitro].
Wide concentration range (10(-14)-10(-4) M) of bis-(n-tributyltin)-oxide effect on Na(+)-dependent uptake, spontaneous and K(+)-stimulated release, specific receptor binding and GABA metabolism were studied in vitro experiments using brain slices, synaptic membrane fraction and brain tissue homogenates. It is shown that the dependence "concentration-effect" is of non-linear character in all cases. Prevailing suppression of Na(+)-dependent uptake and specific receptor binding during K(+)-stimulated release and metabolism (production and utilization) of GABA activation were marked as a general tendency. Mechanisms of TBTO effect on the studied processes and the involvement of GABA-ergic system in realization of TBTO neurotoxic effects are discussed.
Gerasimiak
G R
GR
Rozanov
V A
VA
Shafran
L M
LM
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000494:pharmacology; D013570:Synaptic Membranes / Q000187:drug effects* / Q000378:metabolism; D014220:Trialkyltin Compounds / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D014220:Trialkyltin Compounds; C005961:bis(tri-n-butyltin)oxide; D005680:gamma-Aminobutyric Acid; D012964:Sodium; D011188:Potassium
Izuchenie vliianiia bis(n-tributilolovo)-oksida na GAMK-ergicheskuiu sistemu mozga in vitro.