Trypsin-like activity levels of Treponema denticola and Porphyromonas gingivalis in adults with periodontitis.
Treponema denticola (Td) and Porphyromonas gingivalis (Pg) are associated with human moderate and severe adult periodontal diseases. This study quantifies these two anaerobes and their trypsin-like (TL) activities in subgingival plaque collected from both clinically healthy and periodontally diseased sites of human periodontitis patients. Antigen levels of the microorganisms were determined by monoclonal antibodies and TL activities were measured by the fluorescent substrate Z-gly-gly-arg-AFC in a disc format. Significant positive correlations were observed between the antigen levels and the TL activities when the data were subjected to statistical analyses both on a site-specific and on a patient basis. Anaerobe synergism was found between Td and Pg in a continental US population, and positive correlations were found between anaerobe levels (individually and total) and clinical indicators of adult periodontitis.
Pederson
E D
ED
US Naval Dental Research Institute, Great Lakes, IL.
Miller
J W
JW
Matheson
S
S
Simonson
L G
LG
Chadwick
D E
DE
Covill
P J
PJ
Turner
D W
DW
Lamberts
B L
BL
Morton
H E
HE
D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins; D015169:Colony Count, Microbial; D003374:Coumarins / Q000378:metabolism; D003546:Cysteine Endopeptidases; D003773:Dental Plaque / Q000201:enzymology / Q000382:microbiology; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000382:microbiology / Q000473:pathology; D010518:Periodontitis / Q000201:enzymology / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000201:enzymology* / Q000276:immunology / Q000302:isolation & purification; D013559:Symbiosis; D014208:Treponema / Q000201:enzymology* / Q000276:immunology / Q000302:isolation & purification; D014357:Trypsin / Q000378:metabolism*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003374:Coumarins; C055467:7-amino-4-trifluoromethylcoumarin; D014357:Trypsin; D003546:Cysteine Endopeptidases; C078912:prtT protein, Porphyromonas gingivalis