An improved method for analysis of technetium radiolabeled antibody by size exclusion HPLC.
A quantitative radio HPLC method for the analysis of radiolabeled 99mTc compounds, in particular labeled antibodies or proteins, is described. The method is based on the quantitative re-oxidation of the reduced column absorbed 99mTc species to pertechnetate using hydrogen peroxide. The pertechnetate is quantitatively eluted from the column. The method can be used as a stand alone technique for determining the true radiolabeling yield of labeled proteins/antibodies. The method has been automated for routine use and the typical analysis time is 30 min.
Muddukrishna
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Biomira Inc., Edmonton, Alberta, Canada.
Jorge
P
P
Machner
W
W
Sykes
T R
TR
Noujaim
A A
AA
D000911:Antibodies, Monoclonal / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D008970:Molecular Weight; D011933:Reagent Kits, Diagnostic; D013667:Technetium
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D013667:Technetium