[Design of mixed polymers based on DNA fragments with consensus promotor elements, separated by nonnucleotide segments].
Mixed oligomers, representing oligonucleotides connected with long non-nucleotide spacers, have been synthesized using phosphoramidite chemistry. The oligonucleotide moieties of the mixed oligomers fully or partially correspond in the structure to the consensus elements of -35 (TTGACA) and -10 (TATAATG) regions of prokaryotic promoters. The non-nucleotide spacers, approximating in size 17-membered DNA fragments, were synthesized using phosphoramidite derivatives of polyethylene glycol (PEG600), tetraethylene glycol or dodecanediol. It is shown that the oligonucleotide moieties of the mixed oligomers can form "normal" DNA like antiparallel complementary complexes, being the substrates of T4 DNA ligase. To obtain the DNA-like polymers with alternating natural and non-natural regions or cyclic structures, the enzymatic ligation of different complexes of the oligomers synthesized was studied.
Koroleva
O N
ON
Volkov
E M
EM
Drutsa
V L
VL
D001345:Autoradiography; D001483:Base Sequence; D001704:Biopolymers; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D011088:DNA Ligases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013379:Substrate Specificity
D001704:Biopolymers; D004247:DNA; D011088:DNA Ligases
Konstruirovanie smeshannykh polimerov na osnove fragmentov DNK s konsensusnymi élementami promotorov, razdelennykh nenukleotidnymi uchastkami.