Length of uninterrupted CGG repeats determines instability in the FMR1 gene.
Analysis of 84 human X chromosomes for the presence of interrupting AGG trinucleotides within the CGG repeat tract of the FMR1 gene revealed that most alleles possess two interspersed AGGs and that the longest tract of uninterrupted CGG repeats is usually found at the 3' end. Variation in the length of the repeat appears polar. Alleles containing between 34 and 55 repeats, with documented unstable transmissions, were shown to have lost one or both AGG interruptions. These comparisons define an instability threshold of 34-38 uninterrupted CGG repeats. Analysis of premutation alleles in Fragile X syndrome carriers reveals that 70% of these alleles contain a single AGG interruption. These data suggest that the loss of an AGG is an important mutational event in the generation of unstable alleles predisposed to the Fragile X syndrome.
Eichler
E E
EE
Department of Molecular & Human Genetics, Baylor College of Medicine, Houston, Texas 77030.
Holden
J J
JJ
Popovich
B W
BW
Reiss
A L
AL
Snow
K
K
Thibodeau
S N
SN
Richards
C S
CS
Ward
P A
PA
Nelson
D L
DL
D001483:Base Sequence; D004247:DNA; D051860:Fragile X Messenger Ribonucleoprotein 1; D006801:Humans; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins; D012091:Repetitive Sequences, Nucleic Acid; D014960:X Chromosome
D004247:DNA; D051860:Fragile X Messenger Ribonucleoprotein 1; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; C497683:FMR1 protein, human; C022262:Okazaki fragments