[The alteration of histidase catalytic activity and the expression of the enzyme protein in rat primary hepatomas].
The catalytic activity of histidase and the expression of the enzyme protein were investigated in rat hepatomas induced by diethylnitrosamine. The results indicated that the activities of histidase in the hepatomas were significantly diminished as compared with normal rat livers (P < 0.01). Western blot analysis revealed that enzyme protein levels in hepatomas were markedly reduced. It is thus inferred that some factors which control expression of histidase protein may be altered during carcinogenesis. These findings lay a foundation for studying the relationship between the regulation and expression of the histidase gene and hepato carcinogenesis.
Zhao
Q
Q
Cancer Institute, CAMS, Beijing.
Zhao
M
M
Zhang
C
C
D000818:Animals; D015153:Blotting, Western; D004052:Diethylnitrosamine; D005260:Female; D006638:Histidine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D051381:Rats
D004052:Diethylnitrosamine; D006638:Histidine Ammonia-Lyase