Characterization and localization of the TCL-1 oncogene product.
The TCL-1 gene maps at chromosome 14q32.1 and is activated in T cell leukemias and lymphomas by either chromosome translocations or inversions that juxtapose the TCL-1 gene to the alpha/delta or the beta locus of the T cell receptor. The open reading frame of the TCL-1 gene, coding for a protein of 114 amino acids, was expressed in bacteria and antisera were raised against it. The antibodies recognized the predicted TCL-1 M(r) 14,000 protein product in cells expressing TCL-1 mRNA. Cell fractionation experiments indicated that the TCL-1 protein is present in the microsomal fraction. These results were confirmed by confocal microscopy. The TCL-1 protein has considerable sequence similarities to the product of the MTCP-1 gene on chromosome Xq28, which is involved in T cell lympho-proliferative diseases. Thus, TCL-1 may represent a member of a novel family of genes involved in lymphoid proliferation and/or survival and in T cell malignancies.
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Jefferson Cancer Institute, Jefferson Medical College, Philadelphia, Pennsylvania 19107.
Virgilio
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Narducci
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Facchiano
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A
Russo
G
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Croce
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CM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D006801:Humans; D015448:Leukemia, B-Cell / Q000235:genetics; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics; D017433:Protein Structure, Secondary; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured; D014960:X Chromosome / Q000235:genetics
D011518:Proto-Oncogene Proteins; C090851:TCL1A protein, human