title: Evaluation of topically administered tissue plasminogen activator for intraocular fibrinolysis in dogs.
abstract: Fibrin clots were induced in eyes of dogs by injection of autogenous citrated plasma into the anterior chamber. Twenty-four hours after clot formation, one 50-microliters drop of tissue plasminogen activator at a concentration of 5 mg/ml (group 1, n = 7) was administered topically 9 times at 5-minute intervals, or a collagen shield that was hydrated with tissue plasminogen activator at a concentration of 5 mg/ml (group 2, n = 7) was applied. The contralateral eye served as a nontreated control. Serial photographs were taken of the fibrin clots after topical application of tissue plasminogen activator. Computerized morphometric analysis was then used to evaluate changes in cross-sectional surface area of the fibrin clot. There was no significant mean percentage decrease in clot surface area of treated eyes of group-1 dogs or in treated eyes of group-2 dogs. In addition, there was no significant difference in mean percentage decrease in clot surface area between treated eyes of group-1 and group-2 dogs.
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana 61801.
authors.forename: P A
authors.initials: PA
authors.lastname: Gerding
authors.forename: R E
authors.initials: RE
authors.lastname: Hamor
authors.forename: D T
authors.initials: DT
authors.lastname: Ramsey
authors.forename: S
authors.initials: S
authors.lastname: Vasaune
authors.forename: D J
authors.initials: DJ
authors.lastname: Schaeffer
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000867:Anterior Chamber; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D005128:Eye Diseases / Q000188:drug therapy / Q000662:veterinary*; D005260:Female; D005342:Fibrinolysis / Q000187:drug effects*; D008297:Male; D009883:Ophthalmic Solutions; D010959:Tissue Plasminogen Activator / Q000008:administration & dosage* / Q000627:therapeutic use
chemical_list: D009883:Ophthalmic Solutions; D010959:Tissue Plasminogen Activator