title: Methoxypsoralen phototherapy of transitional cell carcinoma.
abstract: OBJECTIVE
  The goal of this research was to assess whether methoxypsoralen compounds in combination with ultraviolet light were effective in preventing cellular proliferation in an in vitro model of human transitional cell carcinoma.
  
  
  METHODS
  Three methoxypsoralen compounds, 5-methoxypsoralen (5-MOP), 8-methoxypsoralen (8-MOP), and 4'-aminomethyl 4,5'-8'-trimethylpsoralen (AMT), were added in vitro to T-24 transitional cell carcinoma cells. Psoralens directly bind to DNA, cross-linking the strands when exposed to ultraviolet light and thereby prevent cellular division.
  
  
  RESULTS
  In vitro activity was demonstrated utilizing AMT and ultraviolet radiation at 320 to 340 nm, preventing cellular proliferation in T-24 transitional cell carcinoma.
  
  
  CONCLUSIONS
  Methoxypsoralen compounds in combination with ultraviolet light are effective in preventing proliferation of bladder carcinoma cells in vitro. This therapy may prove to be effective in clinical early stage transitional cell carcinoma and warrants further assessment.
authors.affiliation: Department of Surgery, Emory University School of Medicine, Atlanta, Georgia.
authors.forename: T E
authors.initials: TE
authors.lastname: Keane
authors.forename: J A
authors.initials: JA
authors.lastname: Petros
authors.forename: B
authors.initials: B
authors.lastname: Velimirovich
authors.forename: K T
authors.initials: KT
authors.lastname: Yue
authors.forename: S D
authors.initials: SD
authors.lastname: Graham
mesh_terms: D000078223:5-Methoxypsoralen; D002295:Carcinoma, Transitional Cell / Q000473:pathology*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008730:Methoxsalen / Q000031:analogs & derivatives / Q000494:pharmacology*; D014307:Trioxsalen / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D014466:Ultraviolet Rays; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
chemical_list: D000078223:5-Methoxypsoralen; D008730:Methoxsalen; D014307:Trioxsalen