title: Cloning and expression of the axolotl proto-oncogene ski.
abstract: In vitro and in vivo overexpression studies have demonstrated that the c-ski proto-oncogene can influence proliferation, morphological transformation and myogenic differentiation. We report the isolation and expression of an axolotl (Ambystoma mexicanum) c-ski (aski) gene. Sequence analysis revealed a high degree of nucleotide and predicted amino acid (AA) homology with mammalian and anuran c-ski, showing the highest conservation to Xenopus laevis c-ski (74% nucleotide and 87% AA). Northern analysis showed that axolotl c-ski is expressed in unfertilized eggs and at increasing levels in embryos from blastula to tadpole stage. c-ski expression was also detected in larval limb muscle and in several stages of regenerating limb blastemas. These data indicate that axolotl c-ski is highly conserved among amphibians and mammals and suggests that it plays a role in urodele embryogenesis and limb regeneration.
authors.affiliation: Department of Biology, Indiana University, Bloomington 47405.
authors.forename: D C
authors.initials: DC
authors.lastname: Ludolph
authors.forename: A W
authors.initials: AW
authors.lastname: Neff
authors.forename: M A
authors.initials: MA
authors.lastname: Parker
authors.forename: A L
authors.initials: AL
authors.lastname: Mescher
authors.forename: R C
authors.initials: RC
authors.lastname: Smith
authors.forename: G M
authors.initials: GM
authors.lastname: Malacinski
mesh_terms: D000557:Ambystoma / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D014982:Xenopus laevis
chemical_list: D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; C060614:SKI protein, human