DNA binding and transactivation activity of A-myb, a c-myb-related gene.
A partial-length A-myb complementary DNA recently cloned by low-stringency hybridization with a c-myb probe to complementary DNA libraries derived from human cell lines showed a high degree of homology with the DNA-binding domain of c-myb and B-myb, suggesting that A-myb also encoded a DNA-binding protein. We report here the sequence of the entire coding region of A-myb complementary DNA and show that the full-length GST-A-myb fusion protein or a truncated derivative corresponding only to the putative DNA-binding domain interacts specifically with Myb-binding sites of the c-myb responsive promoters, MIM-1 and CD34. In transient transfection assays, A-myb transactivated the bound promoters. These results suggest that, analogous to the other members of the Myb family, the A-myb gene encodes a bona fide transactivator. The distinct function of A-myb might derive from its pattern of expression and/or its relative potency as a transactivator of myb target genes.
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Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Calabretta
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics* / Q000502:physiology; D005809:Genes, Regulator / Q000235:genetics* / Q000502:physiology; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009857:Oncogenes / Q000235:genetics* / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
D018076:DNA, Complementary; C088831:MYBL1 protein, human; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D015500:Chloramphenicol O-Acetyltransferase