Genes encoding homologues of three consecutive enzymes in the butyrate/butanol-producing pathway of Clostridium acetobutylicum are clustered on the Clostridium difficile chromosome.
Screening of a Clostridium difficile lambda EMBL3 gene library with antisera raised against C. difficile culture supernatant identified several clones expressing a 31-kDa protein. A 1.8-kb HindIII fragment subcloned from one of the clones was sufficient for expression of the 31-kDa polypeptide. Southern blot analysis showed a region homologous to this fragment to be present in all of 13 different C. difficile strains tested. Sequence analysis of the 1.8-kb fragment revealed three adjacent open reading frames. A database search showed that these three open reading frames appeared to encode homologues of three consecutive enzymes in the butanol/butyrate-producing pathway of Clostridium acetobutylicum (crotonase, beta-hydroxybutyryl coenzyme A dehydrogenase and thiolase).
Mullany
P
P
Department of Medical Microbiology, St. Bartholomew's Hospital Medical College, West Smithfield, London, UK.
Clayton
C L
CL
Pallen
M J
MJ
Slone
R
R
al-Saleh
A
A
Tabaqchali
S
S
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics; D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D000440:Butanols / Q000378:metabolism; D002087:Butyrates / Q000378:metabolism; D020148:Butyric Acid; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D016360:Clostridioides difficile / Q000201:enzymology / Q000235:genetics*; D003013:Clostridium / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D004746:Enoyl-CoA Hydratase / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames / Q000235:genetics; D010084:Oxidation-Reduction
D000440:Butanols; D002087:Butyrates; D005227:Fatty Acids; D020148:Butyric Acid; D015094:3-Hydroxyacyl CoA Dehydrogenases; C021388:3-hydroxybutyryl-CoA dehydrogenase; D000101:Acetyl-CoA C-Acetyltransferase; D004746:Enoyl-CoA Hydratase