title: Enzyme-linked immunosorbent assay for measuring ileal symbiont intracellularis-specific immunoglobulin G response in sera of pigs.
abstract: Proliferative enteritis (PE) is a common intestinal disease on pig farms. The disease is caused by ileal symbiont (IS) intracellularis (Campylobacter-like organisms) bacteria. An enzyme-linked immunosorbent assay (ELISA) was developed to measure IS intracellularis-specific immunoglobulin G (IgG) response in the sera of pigs. The antigen used in the ELISA was filtered, percoll gradient-purified IS intracellularis extracted from the intestines of pigs affected with proliferative hemorrhagic enteropathy. The antibody responses of pigs challenged with intestinal homogenates from pigs affected with proliferative hemorrhagic enteropathy containing IS intracellularis or percoll-gradient purified IS intracellularis were low and variable. The low IgG titers measured in challenged pigs support previous findings that IgG plays a minor role in the immune response of pigs to IS intracellularis. On a farm in which infection was endemic, pigs seroconverted at between 7 and 24 weeks of age. High IgG titers, indicative of maternally acquired antibody, were present in 3-week-old pigs. The IgG titers in piglets were lowest at 6 weeks of age, which approximates the age of onset of clinical disease. These results suggest that IgG plays a role in determining the susceptibilities of pigs to natural infection. Measurements of seroconversion by the ELISA might aid in epidemiological investigations of PE in naturally infected herds. However, the variable antibody responses in experimentally challenged pigs would seem to limit its usefulness as an antemortem diagnostic test for PE.
authors.affiliation: Regional Veterinary Laboratory, Department of Agriculture, Bendigo, Victoria, Australia.
authors.forename: P K
authors.initials: PK
authors.lastname: Holyoake
authors.forename: R S
authors.initials: RS
authors.lastname: Cutler
authors.forename: I W
authors.initials: IW
authors.lastname: Caple
authors.forename: R P
authors.initials: RP
authors.lastname: Monckton
mesh_terms: D000367:Age Factors; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000917:Antibody Formation; D002169:Campylobacter Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004751:Enteritis / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007082:Ileum / Q000382:microbiology; D007074:Immunoglobulin G / Q000097:blood*; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D013559:Symbiosis
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G