Immunochemical study of polysaccharide antigen in Streptococcus sobrinus and Streptococcus downei with a cross-reactive monoclonal antibody.
A monoclonal antibody (mAb h-448) was prepared after cell fusion of mouse myeloma cells (SP2/0-Ag-14) to the spleen cells of mice immunised with serotype h strain (MF25) of Streptococcus downei. The antibody (IgM class) reacted in enzyme immunoassay only with whole cells as well as purified polysaccharide (PS) antigen of Streptococcus sobrinus (types d and g) and Streptococcus downei (serotype h), but not with cells or purified PS antigen from any other serotypes of the mutans group of streptococci. mAb h-448 also quantitatively precipitated in solution with the purified antigens. Competitive hapten inhibition tests demonstrated that beta-methylgalactopyranoside inhibited the reaction most strongly. Although rhamnose also showed a substantial inhibitory effect, the results of this study indicate that the antigenic determinant of the PS antigen has a structure similar to the beta-methylgalactopyranoside molecule.
Ota
F
F
Department of Food Microbiology, School of Medicine, University of Tokushima, Japan.
Kato
H
H
Hirota
K
K
Inoue
H
H
Tomotake
H
H
Uchiyama
C
C
Minato
Y
Y
D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D006241:Haptens; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011233:Precipitin Tests; D013291:Streptococcus / Q000276:immunology*; D016986:Streptococcus sobrinus / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006241:Haptens; D011135:Polysaccharides, Bacterial