Pharmacokinetics and organ distribution of the sorbin C-terminal peptides.
Sorbin is a 153 amino acid peptide isolated from porcine small intestine. The heptapeptide-amide is the minimal active site of the natural molecule. A comparison of the distribution of C-7 and C-20 sorbin, which have been shown to share the activity of sorbin in increasing intestinal absorption of electrolytes, was undertaken by radioimmunoassay, after perfusion of 200 micrograms/kg/h. A longer half-life in plasma was observed for C-20 sorbin than for C-7 sorbin, with a clearance rate of 18 +/- 4 ml/min/kg vs. 40.6 +/- 13.5 ml/min/kg and a distribution volume of 192 +/- 35 ml/kg vs. 286 +/- 123 ml/kg. The accumulation of tritiated C-7 sorbin was observed in enterocytes, serosal acini of the salivary glands, and fundus chief cells. The recovery of intact peptide in the intestine was 0.06% per gram of tissue. Eighteen percent of the peptide was detected in urine.
Nicol
P
P
INSERUM Unité 45, Hôpital Edouard Herriot, Lyon, France.
el Fadil
F A
FA
Charpin
G
G
Jourdan
G
G
Dumas
C
C
Grognet
J M
JM
Tarrade
T
T
Pansu
D
D
Descroix-Vagne
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D006207:Half-Life; D007262:Infusions, Intravenous; D007553:Isotope Labeling; D007583:Jejunum / Q000033:anatomy & histology / Q000737:chemistry; D008297:Male; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010446:Peptide Fragments / Q000493:pharmacokinetics*; D010455:Peptides / Q000097:blood / Q000493:pharmacokinetics*; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D013363:Submandibular Gland / Q000033:anatomy & histology / Q000737:chemistry; D014018:Tissue Distribution; D014316:Tritium
D010446:Peptide Fragments; D010455:Peptides; D014316:Tritium; C071218:sorbin peptide