Characterization and identification of Vagococcus fluvialis strains isolated from domestic animals.
Strains of Vagococcus fluvialis, a species of Gram-positive catalase-negative cocci, related to the genera Enterococcus and Carnobacterium, were isolated from various lesions of pigs, from lesions and tonsils of cattle and cats and from tonsils of a horse. Most lesion strains were isolated in mixed culture from animals with disease conditions unrelated to coccal infection. Certain differences with the species description of Vagococcus fluvialis were found: only a proportion of the strains was motile; many strains gave positive reactions to Voges-Proskauer, alkaline phosphatase and leucine arylamidase tests or produced acid from galactose and D-tagatose. SDS-PAGE of whole-cell protein patterns, however, confirmed the phenotypic identification. Guidelines for identification of Vagococcus fluvialis are given and an emended description of the species is proposed.
Pot
B
B
Laboratory of Microbiology, Faculty of Sciences, Universiteit Gent, Belgium.
Devriese
L A
LA
Hommez
J
J
Miry
C
C
Vandemeulebroecke
K
K
Kersters
K
K
Haesebrouck
F
F
D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D001426:Bacterial Proteins / Q000302:isolation & purification; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000378:metabolism; D002415:Cats; D002417:Cattle; D002455:Cell Division; D002465:Cell Movement; D004591:Electrophoresis, Polyacrylamide Gel; D016983:Enterococcus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D006095:Gram-Positive Cocci / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D006736:Horses; D010641:Phenotype; D013045:Species Specificity; D013552:Swine
D001426:Bacterial Proteins; D002374:Catalase