Antibodies to synthetic peptides based on band 3 motifs react specifically with Plasmodium falciparum (human malaria)-infected erythrocytes and block cytoadherence.
Rabbit polyclonal and mouse monoclonal antibodies (Mabs) prepared against synthetic peptides patterned on exofacial loops 3 (amino acids 546-555) and 7 (821-834) of the human anion transport protein band 3 inhibited the cytoadherence of Plasmodium falciparum-infected erythrocytes to C32 amelanotic melanoma cells. Mabs directed against exofacial loop 4 (amino acids 628-642) did not inhibit adherence to a significant degree. The murine Mabs recognized only P. falciparum-infected cryptic in uninfected erythrocytes.
Crandall
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University of California Riverside 92521.
Sherman
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D000595:Amino Acid Sequence; D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000469:parasitology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D018328:Melanoma, Amelanotic; D051379:Mice; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000276:immunology*; D011817:Rabbits; D012878:Skin Neoplasms; D014407:Tumor Cells, Cultured
D001457:Anion Exchange Protein 1, Erythrocyte; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D007106:Immune Sera