[Analysis of the gene encoding human PC2, a prohormone processing enzyme].
Prohormone Convertase 2 (PC2) is a specific endoprotease responsible for the processing of proinsulin to insulin. PC2 is expressed in pancreatic islets, pituitary and brain but is very low or absent in most other tissues, such as liver, spleen and kidney. To evaluate the regulated expression of the human PC2 gene we have analyzed its structure and characterized its promoter. The gene spans > 130 kilobase pairs and consists of 12 exons. Comparison with the structure of the gene encoding human furin, revealed a high degree of conservation of exon-intron junctions. The hPC2 gene was localized to chromosome 20, band p11.2. The promoter region of the PC2 gene is very G + C rich and contains six potential Sp1 binding sites but no TATA or CAAT box. Analysis of the level of chrolamphenicol acetyltransferase activity with several deletion mutants identified the region from -1100 to -539 from the translation start site as essential for the PC2 promoter activity.
Ohagi
S
S
First Department of Medicine, Wakayama University of Medical Science.
Yoshida
H
H
Nanjo
K
K
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003920:Diabetes Mellitus / Q000209:etiology / Q000235:genetics; D006801:Humans; D007515:Islets of Langerhans / Q000201:enzymology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D043484:Proprotein Convertases; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases / Q000235:genetics*; D013381:Subtilisins / Q000235:genetics*
D029701:Saccharomyces cerevisiae Proteins; D043484:Proprotein Convertases; D012697:Serine Endopeptidases; D013381:Subtilisins; C058751:KEX2 protein, S cerevisiae