Cytokines modulate interleukin-6 production by human salivary gland cell line.
The ability of HrTNF-alpha, HrIL-1 beta, and HrIFN-gamma to modulate IL-6 production by cultured human salivary cell line (HSG) was examined. IL-6 activity was measured by the hybridoma growth factor biologic activity. HrTNF-alpha had a significant dose-dependent effect, whereas HrIL-1 beta and HrIFN-gamma had moderate and minor effects, respectively. Maximal effect (920 units) was noted when cytokines were applied in combination (1000 units HrIFN-gamma and 20 units HrTNF-alpha) and incubated with human salivary gland cells for 72 hr. The same combination of cytokines had also a marked inhibitory effect (70% of control) on human salivary gland cell growth. This is the first report of these concomitant phenomena in a human cell line originating from salivary tissue.
Katz
J
J
Heller Institute, Sheba Medical Center, Tel Aviv, Israel.
Nagler
R
R
Barak
S
S
Livneh
A
A
Baum
B
B
Atkinson
J
J
Shemer
J
J
D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D016207:Cytokines / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology; D015850:Interleukin-6 / Q000096:biosynthesis*; D012469:Salivary Glands / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma