title: Effects of protein isolates from radish and spinach leaves on serum lipids levels in rats.
abstract: Radish and spinach leaf protein isolates (RLP and SLP, respectively) were prepared from chilled aqueous 0.2% sodium hydroxide extract of their leaves. The RLP and SLP, and those supplemented with methionine (RLP+Met and SLP+Met, respectively) to become equal to casein in methionine content, were compared with casein for their effects on serum cholesterol level in rats fed with a cholesterol-enriched diet for 14 days. Each protein isolate was incorporated into the cholesterol-enriched diet to provide a 15% protein level. RLP was extremely inferior to SLP and casein for body weight gain of rats, but that of rats fed with RLP+Met diet was almost equal to that of casein and SLP groups. The serum cholesterol level in rats fed with SLP and SLP+Met diets was significantly lower as compared with that of the casein-fed rats. Both the amounts of excreted cholesterol and bile acids were significantly higher in rats fed with the SLP and SLP+Met diets than that of the casein-fed rats. These results suggest that the hypocholesterolemic action of SLP may in part have been due to the inhibition of intestinal absorption of both cholesterol and bile acids. RLP+Met diet tended to decrease the serum cholesterol level as compared to casein diet, but the difference was not significant.
authors.affiliation: Department of Bioproduction, Faculty of Agriculture, Yamagata University, Tsuruoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.forename: K
authors.initials: K
authors.lastname: Igarashi
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D005243:Feces / Q000737:chemistry; D008055:Lipids / Q000097:blood*; D008099:Liver / Q000378:metabolism; D008297:Male; D014674:Plant Proteins, Dietary / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013256:Steroids / Q000032:analysis
chemical_list: D000596:Amino Acids; D008055:Lipids; D014674:Plant Proteins, Dietary; D013256:Steroids