[Production of oxygenated free radicals and the role of exogenous antioxidants in liver transplantation in rats].
It has been suggested that, in transplantation organs, the lesions observed after conservation then reperfusion could be related to the formation of oxygenated free radicals. The aim of our work was first to verify the hypothesis that oxygenated free radical are formed after ischaemia-reperfusion of the liver, then to evaluate the effects of the allopurinol and glutathion, known antagonists of oxygenated free radicals, contained in the University of Wisconsin conservation fluid. The University of Wisconsin solution was compared with a Collins solution which does not contain oxygenated free radical antagonists. An orthotopic liver transplantation model was used in Wistar rats. Three groups were studied. In the control group, 5 rats underwent laparotomy then were closed with no surgery being performed. In the Wisconsin group (n = 6) and the Collins group (n = 6) the livers were washed and conserved in the corresponding solution at 4 degrees C before transplanting. Plasma levels of malonyldialdehyde, measured by high performance liquid chromatography, were used as a marker for the formation of oxygenated free radicals. Impaired liver function was assessed on the basis of mortality and serum transaminases, alkaline phosphatase and total bilirubin levels. Liver biopsy was performed at sacrifice. The level of malonyldialdehyde was significantly higher in the transplanted groups compared with the control group (p < 0.01). There was no difference between the Wisconsin and the Collins groups. Hepatic function was significantly reduced in the transplanted groups compared with the control group (p < 0.05). There was no significant difference between the Wisconsin and the Collins groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Yandza
T
T
Service de Chirurgie pédiatrique, Hôpital Bicêtre, Le Kremlin-Bicêtre.
Boutron
A
A
Conti
M
M
Fabre
M
M
Lambotte
L
L
Lemonnier
A
A
Martin
E
E
Valayer
J
J
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004195:Disease Models, Animal; D005609:Free Radicals / Q000378:metabolism; D016031:Liver Transplantation; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D005609:Free Radicals; D010100:Oxygen
Production des radicaux libres oxygénés et rôle des antioxydants exogènes en transplantation du foie chez le rat.