title: Ex vivo studies of polymorphonuclear neutrophils from patients with early-onset-periodontitis (II). Chemiluminescence response analysis.
abstract: Previous studies have indicated that oxygen-free radicals may cause damage to the periodontal tissues. This study compared the luminol-dependent chemiluminescence response (after stimulation with either opsonized zymosan or phorbol myristate acetate (PMA) of peripheral blood polymorphonuclear leukocytes (PMN) isolated from human subjects with a healthy periodontium (n = 7), gingivitis (n = 8), adult periodontitis (n = 8), or early-onset periodontitis (n = 17). These results were also compared with those obtained in a larger reference group which consists of 50 subjects without infection or inflammation, selected on the basis of laboratory investigations. An enhanced response was defined as being 2 standard deviations above the reference group mean; a reduced response was defined as being 2 standard deviations below this mean. Although PMN from patients with either gingivitis or periodontitis were often functionally activated (when compared to the PMN from the reference group), no significant differences could be found between the 4 groups, with regard to the chemiluminescence response means obtained in a basal state or after stimulation.
authors.affiliation: Faculté de Chirurgie-Dentaire, Département de Parodontologie, Centre de Recherches Parodontales, Rennes, France.
authors.forename: P
authors.initials: P
authors.lastname: Mouynet
authors.forename: M
authors.initials: M
authors.lastname: Delamaire
authors.forename: M C
authors.initials: MC
authors.lastname: Legoff
authors.forename: B
authors.initials: B
authors.lastname: Genetet
authors.forename: M
authors.initials: M
authors.lastname: Yardin
authors.forename: J F
authors.initials: JF
authors.lastname: Michel
mesh_terms: D000328:Adult; D017668:Age of Onset; D010520:Aggressive Periodontitis / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D005609:Free Radicals / Q000378:metabolism; D005891:Gingivitis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010518:Periodontitis / Q000378:metabolism* / Q000473:pathology; D010519:Periodontium / Q000378:metabolism / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D005609:Free Radicals; D017382:Reactive Oxygen Species; D008165:Luminol; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate