[Effect of over-synthesis of cloned gene products on methanol metabolism in the recombinant methylotrophic yeast Hansenula polymorpha].
As is shown expression homologous (dihydroxyacetone kinase) and heterologous (HBsAg, beta-galactosidase) genes in methylotrophic yeasts Hansenula polymorpha DL1 negatively affects on the growth parameters of a host strain. The reducing of specific growth rate (mu max) and yield of biomass per the unit of a consumed substrate (Yx/s) were found in all recombinant strains grown on methanol. Overproduction of dihydroxyacetone kinase and beta-galactosidase in recombinant H. polymorpha was accompanied by two-fold increasing of the activity of alcohol oxidase, which is the first enzyme of methanol oxidation. Otherwise, the activity of formaldehyde dehydrogenase two-fold decreased in the recombinant strain overproducing HBsAg compared with the host strain. It is suggested that the over-synthesis of foreign proteins requiring an additional energetic and metabolic expenses might reduce the growth parameters and the activities of some enzymes of methanol metabolism in recombinant methylotrophic yeast H. polymorpha.
Gorlatova
N V
NV
Kriukova
E G
EG
D000429:Alcohol Oxidoreductases / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005800:Genes, Fungal; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis; D000432:Methanol / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis; D010843:Pichia / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D001616:beta-Galactosidase / Q000096:biosynthesis
D005656:Fungal Proteins; D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D017853:Phosphotransferases (Alcohol Group Acceptor); C023132:glycerone kinase; D001616:beta-Galactosidase; D000432:Methanol
Vliianie sverkhsinteza produktov klonirovannykh genov na metabolizm metanola u rekombinantnykh metilotrofnykh drozhzheĭ Hansenula polymorpha.