Antisense peptides to the 43-57 region of acylphosphatase and to the 46-60 region of two isoenzymes of a low-M(r) phosphotyrosine protein phosphatase do not interact with the corresponding proteins.
Three peptides complementary to exposed regions of two low-M(r) phosphotyrosine protein phosphatase isoenzymes and of the acylphosphatase muscle isoenzyme have been synthesized. Each peptide was synthesized on two different types of resins; the peptides were anchored to the resins by amide linkages. The peptide resins were checked by amino acid analysis and Edman degradation and directly used for enzyme purification. Despite our attempts, none of the resins was able to bind significant amounts of the corresponding protein, indicating the lack of interaction between the three proteins and the corresponding complementary peptides. This result agrees with many other reports, confirming that the molecular-recognition theory has no general validity.
Stefani
M
M
Department of Biochemical Sciences, University of Florence, Italy.
Bucciantini
M
M
Taddei
N
N
Mincione
G
G
Manao
G
G
Ramponi
G
G
D017766:Acid Anhydride Hydrolases / Q000737:chemistry / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D016375:Antisense Elements (Genetics); D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000302:isolation & purification*; D011506:Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D012984:Software; D000097024:Acylphosphatase
D017766:Acid Anhydride Hydrolases; D016375:Antisense Elements (Genetics); D007527:Isoenzymes; D010455:Peptides; D017027:Protein Tyrosine Phosphatases; D011506:Proteins; D000097024:Acylphosphatase