Characterization of the surface polypeptides of Strongyloides ratti: a comparison of homogonic and heterogonic strains.
Surface iodination, extraction and SDS-PAGE analysis techniques were employed to characterize and compare the surface polypeptides of two strains of Strongyloides ratti. Third stage infective larvae and parasitic adults of homogonic and heterogonic strains were studied using a variety of surface labelling procedures and detergents for the extraction of labelled molecules. Profiles obtained from SDS-PAGE analysis demonstrated that homogonic and heterogonic strains of S. ratti have identical surface antigens.
Power
J D
JD
School of Biological Sciences, University of Portsmouth, Hants, UK.
Harnett
W
W
Jenkins
T
T
D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry*; D000954:Antigens, Surface / Q000737:chemistry; D003840:Deoxycholic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015801:Helminth Proteins / Q000737:chemistry*; D007202:Indicators and Reagents; D007814:Larva / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D017170:Strongyloides ratti / Q000276:immunology*; D014508:Urea / Q000031:analogs & derivatives
D000947:Antigens, Helminth; D000954:Antigens, Surface; D015801:Helminth Proteins; D007202:Indicators and Reagents; D003840:Deoxycholic Acid; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D014508:Urea