title: Post-traumatic activation of draining lymph node cells. II. Proliferative and phenotypic characteristics.
abstract: Proliferative and phenotypic characteristics of cells in regional lymph nodes that drain burn injury were examined in rats on day 3 postburn, i.e. at the time of maximal spontaneous proliferation and of interleukin-2 and accessory cytokine (IL-1 and IL-6) production. The importance of IL-1 in spontaneous proliferation of draining lymph node cells was demonstrated by stimulation of IL-2-driven proliferation by recombinant IL-1 in vitro and by susceptibility of unstimulated proliferation to anti-IL-1 antibodies, while requirements for IL-6 in draining lymph node cell proliferation were less pronounced. Cell surface phenotyping revealed a slightly increased percentage of CD25+ cells in the blast cell population of freshly isolated draining lymph node cells after injury, which increased further during cultivation. Enrichment in CD8+ cells on day 3 following burn injury was demonstrated, while no changes in total cell population and CD4+ cells was noted. This was however preceded by pronounced percentual decrease of total T cells and CD4+ cells and by increases of B cells and MHC class II+ cells on day 1 postburn. Inhibition of draining lymph node cell proliferation by anti-MHC class II antibodies suggested that this proliferation was class II MHC dependent. The contribution of cell proliferation and/or cell influx to day 3 postburn draining lymph node cell activity is discussed.
authors.affiliation: Institute for Medical Research, Military Medical Academy, Belgrade, Yugoslavia.
authors.forename: M
authors.initials: M
authors.lastname: Kataranovski
authors.forename: J
authors.initials: J
authors.lastname: Kucuk
authors.forename: M
authors.initials: M
authors.lastname: Colić
authors.forename: M
authors.initials: M
authors.lastname: Rapajić
authors.forename: D
authors.initials: D
authors.lastname: Lilić
authors.forename: N
authors.initials: N
authors.lastname: Pejnović
authors.forename: A
authors.initials: A
authors.lastname: Dujić
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D015703:Antigens, CD / Q000032:analysis; D002056:Burns / Q000276:immunology / Q000473:pathology*; D002455:Cell Division; D005434:Flow Cytometry; D016130:Immunophenotyping; D007378:Interleukins / Q000494:pharmacology; D008198:Lymph Nodes / Q000473:pathology*; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex / Q000276:immunology; D008297:Male; D051381:Rats; D015375:Receptors, Interleukin-2 / Q000032:analysis
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D007378:Interleukins; D015375:Receptors, Interleukin-2