Molecular cloning of two pigment-dispersing hormone (PDH) precursors in the blue crab Callinectes sapidus reveals a novel member of the PDH neuropeptide family.
A cDNA library was established from the eyestalk ganglia of the blue crab Callinectes sapidus. Screening resulted in the isolation of a clone (497 bp excluding poly(A) tail) which encodes a beta-PDH previously found in several crustacean species. It displays high sequence similarity with a clone isolated from an eyestalk cDNA library of the shore crab Carcinus maenas, indicating the close phylogenetic relationship of both species. A second clone (414 bp exclusive of the poly(A) tail) encodes a novel beta-PDH analog which displays 400-fold less potency in crab bioassays. Both cDNAs encode open reading frames of 234 bp for the prepropeptides, consisting of signal peptides, PDH-precursor-related peptides, and PDH sequences.
Klein
J M
JM
Department of Cellular and Molecular Biology, University of West Florida, Pensacola 32514-5751.
Mohrherr
C J
CJ
Sleutels
F
F
Riehm
J P
JP
Rao
K R
KR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D010455:Peptides / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D009479:Neuropeptides; D010455:Peptides; D011498:Protein Precursors; C027714:melanophore-dispersing hormone