Characterization of alpha-1 adrenoceptor subtypes in human and canine prostate membranes.
Experiments were designed to characterize the predominant subtype of alpha-1 adrenoceptors in human and canine prostate tissue. The chemical (+/-)-beta-([125I]iodo-4-hydroxyphenyl)-ethyl- aminomethyl-tetralone bound in a specific, saturable manner to a single class of binding sites in membranes that expressed recombinant hamster alpha-1B, bovine alpha-1C and rat alpha-1D receptors expressed in rat-1 fibroblasts and to those from prostate tissue. Competition assays with human and canine prostate membranes revealed only a single class of binding sites. Binding affinity in both human and canine prostate most significantly correlated with binding affinity for the recombinant bovine alpha-1C receptor (r = .98 human, .95 canine). Further analysis with leverage plots demonstrated that binding affinity in human and canine prostate tissue is best predicted by binding affinity to recombinant bovine alpha-1C (P < .01 human and P < .001 canine). These data are consistent with a single class of alpha-1 adrenoceptors in human and canine prostate tissue, which is best represented as the alpha-1C subtype.
Goetz
A S
AS
Department of Cellular Biochemistry, Glaxo Research Institute, Research Triangle Park, North Carolina.
Lutz
M W
MW
Rimele
T J
TJ
Saussy
D L
DL
D000818:Animals; D002417:Cattle; D006224:Cricetinae; D004285:Dogs; D006801:Humans; D008297:Male; D010627:Phenethylamines / Q000378:metabolism; D011467:Prostate / Q000737:chemistry*; D051381:Rats; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis / Q000145:classification*; D011994:Recombinant Proteins / Q000378:metabolism; D044024:Tetralones
D010627:Phenethylamines; D018340:Receptors, Adrenergic, alpha-1; D011994:Recombinant Proteins; D044024:Tetralones; C048008:2-(beta-(3-iodo-4-hydroxyphenyl)ethylaminomethyl)tetralone