Molecular cloning and functional expression of a cDNA for mouse squalene synthase.
Using a probe obtained by PCR amplification, a full-length cDNA encoding squalene synthase was isolated from a mouse liver cDNA library. Its nucleotide sequence had an open reading frame fro a 416 amino acid polypeptide (calculated molecular mass, 48 kDa). In vitro transcription of the cDNA followed by in vitro translation produced a protein of the expected size. The deduced amino acid sequence was 93%, 88% and 46% identical to those of the rat, human and budding yeast squalene synthases, respectively. Blotting analyses showed that the mRNA is 1.6 kb in size and that less than two copies of the gene are present in the mouse genome. To establish the enzyme activity, the entire coding region was subcloned into an expression plasmid so that it was in frame with the N-terminal region of beta-galactosidase. Escherichia coli, which was transformed with the recombinant plasmid, expressed high activity of converting farnesyl diphosphate into squalene.
Inoue
T
T
Department of Life Science, Faculty of Science, Himeji Institute of Technology, Hyogo, Japan.
Osumi
T
T
Hata
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014158:Transcription, Genetic
D018076:DNA, Complementary; D013186:Farnesyl-Diphosphate Farnesyltransferase