title: An improved method for analysis of technetium radiolabeled antibody by size exclusion HPLC.
abstract: A quantitative radio HPLC method for the analysis of radiolabeled 99mTc compounds, in particular labeled antibodies or proteins, is described. The method is based on the quantitative re-oxidation of the reduced column absorbed 99mTc species to pertechnetate using hydrogen peroxide. The pertechnetate is quantitatively eluted from the column. The method can be used as a stand alone technique for determining the true radiolabeling yield of labeled proteins/antibodies. The method has been automated for routine use and the typical analysis time is 30 min.
authors.affiliation: Biomira Inc., Edmonton, Alberta, Canada.
authors.forename: S N
authors.initials: SN
authors.lastname: Muddukrishna
authors.forename: P
authors.initials: P
authors.lastname: Jorge
authors.forename: W
authors.initials: W
authors.lastname: Machner
authors.forename: T R
authors.initials: TR
authors.lastname: Sykes
authors.forename: A A
authors.initials: AA
authors.lastname: Noujaim
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D008970:Molecular Weight; D011933:Reagent Kits, Diagnostic; D013667:Technetium
chemical_list: D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D013667:Technetium