Analysis of Epstein-Barr virus BamHI F DNA fragment in nude mouse-passaged nasopharyngeal carcinoma tissues.
Two nude mouse-passaged nasopharyngeal carcinoma (NPC) tissues, NPC-295 and NPC-306, were different in ability to transform cord blood lymphocytes and contained Epstein-Barr virus (EBV) genomes with difference in BamHI F DNA fragment. Four clones containing DNA sequences of the BamHI F fragment (54,853-62,249) were obtained from genomic libraries of NPC-295 and NPC-306 and their partial restriction enzyme maps and sequences were determined. The restriction enzyme maps of EBV DNA at the BamHI F fragment in NPC-295 and NPC-306 appeared to be similar to that of EBV B95-8 strain. However, 8 nucleotide differences were revealed between NPC-295 and the B95-8 strain when 566 nucleotides (55,405-55,970) were compared, and 15 out of 677 nucleotides analyzed (55,410-56,086) were different between NPC-306 and B95-8.
Liu
M Y
MY
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Lin
S F
SF
Hsu
T Y
TY
Chen
J Y
JY
Yang
C S
CS
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D015257:DNA-Cytosine Methylases / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D015183:Restriction Mapping
D004279:DNA, Viral; C042525:DNA modification methylase BamHI; D015257:DNA-Cytosine Methylases