title: Functional analysis of the promoters of phenylalanine ammonia-lyase genes in pea.
abstract: Pycnospore germination fluid of Mycosphaerella pinodes, a fungus pathogenic on pea, contains both an elicitor and a suppressor of the accumulation of pisatin, a major phytoalexin of pea. Transcription of the genes encoding key enzymes in the biosynthesis of pisatin, namely PAL (a gene encoding phenylalanine ammonia-lyase) and CHS (a gene encoding chalcone synthase), was shown to be activated upon the treatment of pea epicotyl tissues with the fungal elicitor and suppressed upon treatment with the fungal suppressor. To investigate the mechanisms underlying activation and suppression of plant defense genes by signal molecules secreted by a fungal pathogen and other stresses, such as ultraviolet (UV) light, we constructed chimeric genes composed of the 5'-flanking regions of two members of the PSPAL family (the genes encoding phenylalanine ammonia-lyase in Pisum sativum) fused to a bacterial gene for chloramphenicol acetyltransferase. Then, the cis-regulatory elements necessary for elicitor-mediated activation and suppressor-mediated suppression were examined in pea protoplasts. Functional analysis of 5' nested deletions of PSPAL1 and PSPAL2 suggested that an enhancer-like element is located in the TATA-distal region (-2,196 to -406) in PSPAL2. A cis-acting element(s) responsible for elicitor-mediated activation was found in the TATA-proximal region (-340 to -95 in PSPAL1; -406 to -158 in PSPAL2), in which the consensus sequence motifs known as box 1, box 2 and box 4 [Yamada et al. (1992) Plant Cell Physiol. 33: 715, Lois et al. (1989) EMBO J. 8: 1641] were present in close proximity. Furthermore, both promoters were activated by UV light but were partially suppressed in response to the fungal suppressor.
authors.affiliation: Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: P
authors.initials: P
authors.lastname: Sriprasertsak
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Hashimoto
authors.forename: H
authors.initials: H
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Shiraishi
mesh_terms: D001203:Ascomycota; D001483:Base Sequence; D002678:Chimera; D018744:DNA, Plant; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects; D017343:Genes, Plant; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014466:Ultraviolet Rays
chemical_list: D018744:DNA, Plant; D010650:Phenylalanine Ammonia-Lyase