title: Serum biochemical changes and chemiluminescent responses of whole blood in Holstein cattle with leukocyte adhesion deficiency.
abstract: Serum biochemical profile and whole blood chemiluminescent (CL) responses in 8 Holstein cattle affected with leukocyte adhesion deficiency (LAD) were evaluated. Concentrations of sodium, chloride and calcium in serum from cattle affected with LAD were significantly (p < 0.05) decreased as compared with controls. The characteristic changes in serum proteins were hypoalbuminemia and hyperglobulinemia, and the concentrations of albumin and gammaglobulin in serum from normal cattle and cattle affected with LAD were significantly (p < 0.01) different. Significantly (p < 0.01) diminished CL indices and prolonged peak time of CL responses in whole blood were detected in cattle affected with LAD. These findings indicate that the CL response associated with iC3b receptor mediated phagocytic activity is impaired in cattle affected with LAD. The whole blood CL assay appeared to be practical and useful for routine evaluation of blood samples from cattle affected with LAD.
authors.affiliation: Department of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagahata
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Oba
authors.forename: S
authors.initials: S
authors.lastname: Minami
authors.forename: H
authors.initials: H
authors.lastname: Noda
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D018821:CD18 Antigens / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D005260:Female; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D008163:Luminescent Measurements; D010587:Phagocytosis; D017463:Receptors, Complement 3b / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D018821:CD18 Antigens; D017463:Receptors, Complement 3b