title: Gas chromatographic-mass spectrometric determination of ibuprofen enantiomers in human plasma using R(-)-2,2,2-trifluoro-1-(9-anthryl)ethanol as derivatizing reagent.
abstract: A relatively rapid, inexpensive, sensitive and stereospecific gas chromatographic-mass spectrometric method was developed for the quantification of S(+) and R(-)-ibuprofen in human plasma. This method uses a commercially available internal standard and has no interference from endogenous substances nor metabolites. The method involves derivatization of ibuprofen enantiomers with optically active R(-)-2,2,2-trifluoro-1-(9-anthryl)ethanol using oxalyl chloride as the coupling reagent. The subsequently formed diastereoisomers are separated by gas chromatography and analysed by mass spectrometry using selected-ion monitoring. The assay is successfully applied to a pharmacokinetic study. The simplicity, sensitivity and precision of the method make it convenient for the quantification of ibuprofen enantiomers in biological samples.
authors.affiliation: Laboratoire de Chimie Thérapeutique, Faculté de Pharmacie, Illkirch, France.
authors.forename: M J
authors.initials: MJ
authors.lastname: Zhao
authors.forename: C
authors.initials: C
authors.lastname: Peter
authors.forename: M C
authors.initials: MC
authors.lastname: Holtz
authors.forename: N
authors.initials: N
authors.lastname: Hugenell
authors.forename: J C
authors.initials: JC
authors.lastname: Koffel
authors.forename: L
authors.initials: L
authors.lastname: Jung
mesh_terms: D000873:Anthracenes; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007052:Ibuprofen / Q000097:blood* / Q000493:pharmacokinetics; D007202:Indicators and Reagents; D013237:Stereoisomerism; D014270:Trifluoroethanol / Q000031:analogs & derivatives
chemical_list: D000873:Anthracenes; D007202:Indicators and Reagents; C090817:2,2,2-trifluoro-1-(9-anthryl)ethanol; D014270:Trifluoroethanol; D007052:Ibuprofen