Detection of canine parvovirus DNA in paraffin-embedded tissues by polymerase chain reaction.
Canine Parvovirus (CPV) is seemingly a 'new' virus which suddenly appeared during the mid-1970's in an epizootic of disease in dogs. The virus is very similar to the feline panleukopenia virus (FPV), and recent studies have underlined the possible emergence of CPV as a variant of a virus from some other carnivore--possibly from FPV (Parrish, 1990). Several conserved amino-acid changes between CPV and FPV isolates have been defined by cloning and sequencing the capsid-protein gene. An alternative to cloning and sequencing the entire capsid-protein gene would be to use PCR amplification of short regions of the gene containing the appropriate variable amino-acid codons. In addition, use of PCR would also facilitate the study of virus samples which cannot be recovered as infectious agents, e.g. after having undergone formalaldehyde fixation and paraffin-embedding procedures. This study reports on the amplification of CPV DNA from 15-year-old tissue sections which have been prepared by formaldehyde or paraformaldehyde-lysine-periodate-glutaraldehyde fixation, using PCR with various primer pairs within the capsid-protein gene of CPV.
Truyen
U
U
Ludwig-Maximilians-Universität, München, Germany.
Platzer
G
G
Parrish
C R
CR
Hänichen
T
T
Hermanns
W
W
Kaaden
O R
OR
D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000821:virology; D002415:Cats; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004279:DNA, Viral