Effect of dietary fat on glomerular lipid composition and angiotensin II receptors.
Nutritional factors are major etiologic determinants which may affect the incidence or severity of hypertension. Since angiotensin II (Ang II) has a role in some forms of hypertension, the influence of dietary lipid composition on glomerular lipid content and on Ang II receptor parameters was determined. Three groups of rats were fed diets containing 14% by weight of 1) beef fat (saturated fatty acid rich), 2) safflower oil (n-6 fatty acid rich), or 3) fish oil (n-3 fatty acid rich), each supplemented with 2% corn oil. A fourth group of rats was fed rat chow which contained 6.5% fat. At the end of 7 weeks, the rats were sacrificed, the kidneys removed, and renal glomeruli isolated using a sieving technique. Feeding diets which varied in the quantity and composition of fatty acids altered glomerular fatty acid composition of n-6 and n-3 fatty acids and the unsaturation indices of glomeruli. The cholesterol content, but not the phospholipid content, was decreased in glomeruli of animals fed the high fat diets. This resulted in a lower cholesterol/phospholipid ratio, indicative of an increase in membrane fluidity. Glomerular binding was determined using 125I-Ang II in a radioreceptor assay. Binding was proportional to glomerular protein and was time dependent. Receptor affinity (Ka) and number were determined by Scatchard analysis of binding inhibition data. Glomerular Ka was significantly lower for animals fed the rat chow as compared to the semisynthetic diets. There was no significant difference in the concentration of Ang II receptors in glomeruli of animals fed the different diets. The data indicate that the induced alteration in glomerular lipid composition is associated with alteration in the binding affinity of glomerular Ang II receptors.
Awad
A B
AB
Nutrition Program, State University of New York at Buffalo, 14214.
Brown
G P
GP
Fink
C S
CS
Helinski
J D
JD
D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D007678:Kidney Glomerulus / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011945:Receptors, Angiotensin / Q000187:drug effects / Q000378:metabolism*
D004041:Dietary Fats; D005227:Fatty Acids; D011945:Receptors, Angiotensin