Characterisation of the cDNA clones of two beta-tubulin genes and their expression in the potato plant (Solanum tuberosum L.).
The cDNA clones of two potato beta-tubulin genes were isolated from a tuberising stolon tip library. Analysis of 20 positive clones showed that they represented one or another of two different but very similar beta-tubulin genes, designated TUBST1 and TUBST2. The expression pattern of beta-tubulin genes in the potato plant was investigated by RNA blot analysis and by RT-PCR. Southern analysis of potato genomic DNA with coding and non-coding beta-tubulin probes revealed that there are multiple beta-tubulin genes in the potato genome and that there is likely to be considerable divergence in the 3' non-coding sequences. Phylogenetic analysis of plant beta-tubulin genes is described.
Taylor
M A
MA
Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Wright
F
F
Davies
H V
HV
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009024:Morphogenesis; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution; D014404:Tubulin / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D014404:Tubulin; C091034:LOC102577624 protein, Solanum tuberosum