Assignment of the human 8.5 H gene to chromosome 5, region 5q35.
The human 8.5 H probe was isolated from a human cerebellum cDNA library with a probe corresponding to the coding region of the murine 8.5 M cDNA. This cDNA isolated from a murine cDNA library constructed from newborn cerebral hemispheres was selected because of its strong expression in embryonic neurons. Consequently the corresponding human gene could be a candidate for hereditary neurodegenerative diseases. The human 8.5 H gene was assigned by somatic hybrid analysis to chromosome 5; this chromosome contains the gene(s) for spinal muscular atrophy (SMA), a group of heritable degenerative diseases that selectively affect the anterior horn motor neuron of the spinal cord. The localization by in situ hybridization of 8.5 H on 5q35 excluded the possibility that this gene is identical to SMA. The SMA gene(s) was (were) known, from linkage analysis, to be in a region (5q11.2-q13.3) very distant from 5q35.
Diriong
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S
Laboratoire de Cytogénétique et de Génétique Oncologiques, UA 1158 Oncologie Moléculaire CNRS, PR2, Institut Gustave-Roussy, Villejuif, France.
Sabéran-Djoneidi
D
D
Lévi-Strauss
M
M
Bernheim
A
A
Nguyen
V C
VC
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D015342:DNA Probes; D018076:DNA, Complementary; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D012377:Rodentia
D015342:DNA Probes; D018076:DNA, Complementary