title: DNA topoisomerase I from Mycobacterium smegmatis.
abstract: DNA topoisomerase I has been purified from Mycobacterium smegmatis to near homogeneity using different column chromatographic techniques. The enzyme activity relaxes form I DNA into form IV DNA, requiring Mg2+, but not ATP or any other cofactors for its activity. Several properties of the enzyme were found to be similar to that of the prototype enzyme, Escherichia coli topoisomerase I.
authors.affiliation: Centre for Genetic Engineering, Indian Institute of Science, Bangalore.
authors.forename: T
authors.initials: T
authors.lastname: Bhaduri
authors.forename: V
authors.initials: V
authors.lastname: Nagaraja
mesh_terms: D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification*; D009161:Mycobacterium / Q000201:enzymology*
chemical_list: D004264:DNA Topoisomerases, Type I