The BE (2)-M17 neuroblastoma cell line synthesizes and secretes corticotropin-releasing factor.
The BE (2)-M17 human neuroblastoma has previously been shown to express corticotropin-releasing factor (CRF) mRNA following retinoic acid treatment. It is demonstrated in this report that both cell extracts and cell incubation medium of retinoic acid-treated BE (2)-M17 cells were shown to contain CRF-like immunoreactivity (CRF-LI) by RIA. CRF-LI secretion and content were also dose-dependently increased by forskolin. In addition, cell extracts were applied to a C18 Vydac column and peak CRF-LI from the collected fractions was shown to coincide in time of elution with peak immunoreactivity seen with oxidized synthetic CRF standard. Thus, in containing the CRF peptide, the BE (2)-M17 cells are useful models for further study of CRF cellular and genetic regulation.
Kasckow
J W
JW
Department of Psychiatry and Behavioral Sciences, Emory University Medical Center, Atlanta, GA 30322.
Parkes
D G
DG
Owens
M J
MJ
Stipetic
M D
MD
Han
J H
JH
Nemeroff
C B
CB
Vale
W W
WW
D001932:Brain Neoplasms / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005576:Colforsin / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism*; D011863:Radioimmunoassay; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D005576:Colforsin; D014212:Tretinoin; D003346:Corticotropin-Releasing Hormone