Plasma factor triggering alternative complement pathway activation by liposomes.
Several plasma components, such as complement (C) components, play a role in the clearance of liposomes from the circulation. The interactions between liposomes and the C system were investigated in this study. Multilamellar vesicle (MLV) liposomes, which were damaged by activation of the complement, became susceptible depending on the density of cetylmannoside (Man) on the liposome membrane, and activation proceeded through the alternative C pathway as observed for liposomes without Man (PC-MLV) (K. Funato et al., Biochim. Biophys. Acta 1103:198-204, 1992). In addition, the capacity of Man-modified liposomes (Man-MLV) to activate the alternative C pathway was abolished by preadsorption of plasma with Man-MLV but not with PC-MLV. The results suggest that a specific plasma factor adsorbed with Man-MLV was responsible for the augmentation of the C activation and, further, that the rapid clearance of Man-MLV from the circulation is caused by both enhanced C-mediated liposome permeability and enhanced C-mediated phagocytosis of liposomes.
Funato
K
K
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Yamashita
C
C
Kamada
J
J
Tominaga
S
S
Kiwada
H
H
D000818:Animals; D003170:Complement Pathway, Alternative / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D008081:Liposomes / Q000494:pharmacology*; D008297:Male; D008362:Mannosides / Q000494:pharmacology; D008567:Membranes, Artificial; D010587:Phagocytosis / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D008081:Liposomes; D008362:Mannosides; D008567:Membranes, Artificial; C069589:cetylmannoside