Expression of the IL-2 receptor gamma chain on various populations in human peripheral blood.
We have established two rat mAbs, TUGh4 and TUGh5, specific for the human gamma chain of the IL-2 receptor (IL-2R), which is known to be shared among receptors for IL-2, IL-4 and IL-7. The antibodies bound to cell lines transfected with the human gamma chain gene but not to their parental cell lines, and precipitated 65-70 and 80-90 kDa cell surface molecules from lysates of human T cells surface-labeled with Na125I and chemically cross-linked with [125I]IL-2 respectively. Flow cytometry with TUGh4 and TUGh5 detected the gamma chain in a wide variety of peripheral blood cell populations including CD4+ T cells, CD8+ T cells, CD20+ B cells, CD56+ natural killer cells, CD14+ monocytes and granulocytes, contrasting with expression of the alpha and beta chains of IL-2R.
Ishii
N
N
Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
Takeshita
T
T
Kimura
Y
Y
Tada
K
K
Kondo
M
M
Nakamura
M
M
Sugamura
K
K
D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D011233:Precipitin Tests; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015375:Receptors, Interleukin-2