title: Radiation-induced genomic instability.
abstract: Quantitative assessment of the heritable somatic effects of ionizing radiation exposures has relied upon the assumption that radiation-induced lesions were 'fixed' in the DNA prior to the first postirradiation mitosis. Lesion conversion was thought to occur during the initial round of DNA replication or as a consequence of error-prone enzymatic processing of lesions. The standard experimental protocols for the assessment of a variety of radiation-induced endpoints (cell death, specific locus mutations, neoplastic transformation and chromosome aberrations) evaluate these various endpoints at a single snapshot in time. In contrast with the aforementioned approaches, some studies have specifically assessed radiation effects as a function of time following exposure. Evidence has accumulated in support of the hypothesis that radiation exposure induces a persistent destabilization of the genome. This instability has been observed as a delayed expression of lethal mutations, as an enhanced rate of accumulation of non-lethal heritable alterations, and as a progressive intraclonal chromosomal heterogeneity. The genetic controls and biochemical mechanisms underlying radiation-induced genomic instability have not yet been delineated. The aim is to integrate the accumulated evidence that suggests that radiation exposure has a persistent effect on the stability of the mammalian genome.
authors.affiliation: Life Sciences Division, Lawrence Berkeley Laboratory, Berkeley, CA 94720.
authors.forename: A
authors.initials: A
authors.lastname: Kronenberg
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D005796:Genes / Q000528:radiation effects*; D006801:Humans; D009154:Mutation; D009381:Neoplasms, Radiation-Induced / Q000209:etiology*; D011995:Recombination, Genetic