Analysis of immunogenic properties of nonapeptide TVGRGDPHQ from Bordetella pertussis filamentous hemagglutinin.
Immunogenic properties of TVGRGDPHQ nonapeptide which is correspondent to the region 1094-1102 of B. pertussis filamentous hemagglutinin (FHA) were studied. The conjugate of bovine serum albumin with nonapeptide was used for immunization of BALB/c and CBA mice. Antisera of the both lines of mice cross-reacted with a number of antigens, but using affinity chromatography peptide and FHA specific antibodies were extracted. Affinity purified rabbit antibodies to TVGRGPHQ which recognize FHA were also obtained. Therefore the antibodies to the peptide which placed RGD-containing region responsible for macrophage CR3-integrin interaction are capable to distinguish the native antigen. Thus these data are an additional evidence for the nonapeptide use as a component of synthetic vaccine against whooping-cough.
Kavoon
E M
EM
A.V. Palladin Institute of Biochemistry, Academy of Sciences of Ukraine, Kyiv.
Pkhakadze
E G
EG
Marinets
A V
AV
Chechot
V A
VA
Radavsky
J L
JL
Komissarenko
S V
SV
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001886:Bordetella pertussis / Q000737:chemistry*; D003429:Cross Reactions; D006388:Hemagglutinins; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D006388:Hemagglutinins; D009842:Oligopeptides; D012710:Serum Albumin, Bovine