A reversed-phase high-performance liquid chromatographic method for the determination of Clanfenur in rat and human plasma.
A selective and specific high-pressure liquid chromatographic (HPLC) method for the simultaneous assay of Clanfenur and its metabolites in biological fluids of interest has been developed which is suitable for routine analysis, using micro volumes (0.1 ml) of plasma samples only. After protein precipitation the extract is analysed by reversed-phase HPLC with UV detection. Excellent recovery, linearity, accuracy and precision (less than 5% for plasma) are achieved by the assay which is able to quantify Clanfenur and its metabolites in plasma at concentrations between 0.025 and 5.0 mg l-1.
Noteborn
H P
HP
Division of Experimental Therapy, Netherlands Cancer Institute, Amsterdam.
Varossieau
F J
FJ
Monshouwer
M
M
Brands
R
R
Pinedo
H M
HM
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004132:Diflubenzuron / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D004132:Diflubenzuron; C086806:clanfenur