Comparison of the effects of caffeine and other methylxanthines on [Ca2+]i in rat ventricular myocytes.
1. The effects of caffeine and other methylxanthines were investigated on intracellular calcium concentration ([Ca2+]i) and contraction in rat isolated ventricular myocytes. The use of the fluorescent indicator, Indo-1, allowed simultaneous measurement of [Ca2+]i and the intracellular concentration of the methylxanthines. 2. Rapid application of caffeine (10 mM) produced a transient rise of [Ca2+]i which decayed to resting levels. This was accompanied by a transient contraction which decayed to a level above baseline. The addition of theophylline also produced a transient increase of [Ca2+]i. However, following the initial transient, contraction decayed before redeveloping to a maintained level. 3. Direct measurements showed that [caffeine]i rose more quickly than did [theophylline]i. The slower rise of [theophylline]i was associated with a delay in the increase of [Ca2+]i. At lower concentrations of the methylxanthines, theophylline was less effective than caffeine at initiating Ca release. The rate of entry of theobromine was similar to that of theophylline. 4. Isocaffeine did not produce a rise of [Ca2+]i. The rate of rise of [isocaffeine]i was much slower than that of either caffeine or theophylline. 5. Measurements of the oil:water partition coefficient showed that the order of relative partitioning into oil was: caffeine > theophylline > theobromine > isocaffeine. This is similar to the order of rate of entry into the cell. 6. We conclude that many of the differences in the effects of these methylxanthines can be attributed to differences in membrane permeability due to differences in oil:water partition.
Donoso
P
P
Department of Veterinary Preclinical Sciences, University of Liverpool.
O'Neill
S C
SC
Dilly
K W
KW
Negretti
N
N
Eisner
D A
DA
D000818:Animals; D002110:Caffeine / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005456:Fluorescent Dyes; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D012995:Solubility; D013805:Theobromine / Q000493:pharmacokinetics / Q000494:pharmacology; D013806:Theophylline / Q000493:pharmacokinetics / Q000494:pharmacology; D014970:Xanthines / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*
D005456:Fluorescent Dyes; D014970:Xanthines; D002110:Caffeine; D013806:Theophylline; D013805:Theobromine; D002118:Calcium