title: Experimental arthritis and uveitis in rats associated with Mycobacterium butyricum.
abstract: OBJECTIVE
  To determine if the anterior uveitis associated with adjuvant arthritis (AA) in the rat can be passively transferred with arthritis to syngeneic recipients using spleen cells or T cell lines prepared from animals given complete Freund's adjuvant (CFA) and Mycobacterium butyricum (M. butyricum) in incomplete Freund's adjuvant (IFA).
  
  
  METHODS
  Spleen cells from Lewis or Lewis SsN rats given IFA, CFA, type I collagen in IFA (CI-IFA), or type II collagen in IFA (CII-IFA) were administered to naive rats or rats treated with pertussis toxin or bacterial endotoxin. Three CD4+ T cell lines, propagated from CFA injected rats and maintained in vitro with M. butyricum (M-1), bovine proteoglycan (PR-1) or an extract of M. butyricum (MBE-1) were administered to naive or immunosuppressed rats. The arthritogenic and uveitogenic properties of these cell preparations and intradermal MBE-IFA, CII-IFA and intraperitoneal (ip) M. butyricum without adjuvant were evaluated.
  
  
  RESULTS
  Uveitis was observed in 15/69 (22%) arthritic rats given CFA. Spleen cells prepared from CFA injected rats caused arthritis in 55 (82%) and uveitis in 2 (3%) of 67 cell recipients. Uveitis occurred in 2/6 cell recipients pretreated with bacterial endotoxin. Neither uveitis nor arthritis was observed in rats given IFA (0/6) or spleen cells prepared from rats given IFA (0/27), CI-IFA (0/6), or CII-IFA (0/28). CII-IFA produced polyarthritis in 5/6 rats, but no uveitis. CII-IFA induced arthritis associated uveitis in 1/15 animals receiving spleen cells from rats given CII-IFA, but not those given CI-IFA (0/3) or IFA (0/13). Uveitis was observed in one recipient of the M-1 T cell line and in 2 recipients of the PR-1 T cell line. Immunization with 400 micrograms of MBE-IFA induced uveitis but not arthritis in 3/11 animals. The MBE specific T cell line was neither arthritogenic nor uveitogenic. A high frequency (5/6) of uveitis accompanied arthritis in male Lewis rats given ip M. butyricum. Arthritis occurred in 4/10 female Lewis rats given ip M. butyricum and 2 arthritic animals also developed uveitis.
  
  
  CONCLUSIONS
  Uveitis occurs infrequently in arthritic rats given spleen cells from CFA injected animals. The ip administration of M. butyricum constitutes a novel disease model in which the immunopathological relationships between arthritis and uveitis may be more reliably studied.
authors.affiliation: Department of Pediatrics, University of British Columbia, Vancouver, Canada.
authors.forename: R E
authors.initials: RE
authors.lastname: Petty
authors.forename: D W
authors.initials: DW
authors.lastname: Hunt
authors.forename: D M
authors.initials: DM
authors.lastname: Mathers
authors.forename: A Q
authors.initials: AQ
authors.lastname: McCormick
authors.forename: H
authors.initials: H
authors.lastname: Barker
authors.forename: T R
authors.initials: TR
authors.lastname: Southwood
authors.forename: L
authors.initials: L
authors.lastname: Corson
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D003094:Collagen / Q000008:administration & dosage; D004195:Disease Models, Animal; D004731:Endotoxins / Q000008:administration & dosage; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007116:Immunization, Passive; D008297:Male; D009161:Mycobacterium / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D014605:Uveitis / Q000276:immunology*
chemical_list: D004731:Endotoxins; D003094:Collagen; D005620:Freund's Adjuvant