title: Determination of flunitrazepam in plasma by liquid chromatography.
abstract: A reversed-phase liquid chromatographic method has been used to determine flunitrazepam in plasma. Extraction was simple and there was no need to hydrolyse the drug. Separation was achieved on a 150 x 3.9 mm i.d. column packed with 4-microns Nova Pack C18 using a mobile phase of water-acetonitrile-triethylamine (700:300:4, v/v/v) (adjusted to pH 7.5 with orthophosphoric acid). The method was shown to be rapid and reliable with a lower limit of detection of 5 ng ml-1. Results are reported of simple experiments on the effects of temperature and light on the stability of flunitrazepam in plasma kept on the laboratory bench.
authors.affiliation: Department of Clinical Pharmacology and Toxicology, CHU, Bordeaux, France.
authors.forename: F
authors.initials: F
authors.lastname: Benhamou-Batut
authors.forename: F
authors.initials: F
authors.lastname: Demotes-Mainard
authors.forename: L
authors.initials: L
authors.lastname: Labat
authors.forename: G
authors.initials: G
authors.lastname: Vinçon
authors.forename: B
authors.initials: B
authors.lastname: Bannwarth
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005445:Flunitrazepam / Q000097:blood* / Q000506:poisoning; D006801:Humans; D013048:Specimen Handling; D013696:Temperature
chemical_list: D005445:Flunitrazepam