Comparison of polyacrylamide gel electrophoresis & silver staining method with ELISA for rotavirus detection.
A total of 421 human fecal samples were examined for the presence of rotavirus by enzyme-linked immunosorbent assay (ELISA) and by a modified polyacrylamide gel electrophoretic (PAGE) analysis of viral genome. Similar results by both methods were seen in 412 (97.86%) samples. The proportion of ELISA + PAGE - samples (2/421) was lower than the proportion of ELISA - PAGE + samples (7/421). This difference was not significant. The modified PAGE technique for the detection of viral RNA was found to be a rapid, simple, reliable and less expensive technique.
Chakravarti
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A
Department of Microbiology, Maulana Azad Medical College, New Delhi.
Hakim
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Z
D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D012401:Rotavirus / Q000302:isolation & purification*; D016622:Silver Staining