[Effect of panaxadiol saponin (PDS) on phorbol ester induced change of protein kinase C activity in cardiomyocytes].
Partially purified protein kinase C and protein kinase A were separately incubated with various concentrations of PDS (1-1000 micrograms.ml-1) for 10 min in vitro. The results indicate that protein kinase C activity was inhibited in a dose-dependent manner by PDS, but type I and type II protein kinase A were not. In cardiomyocytes preincubated with PDS (250, 500, 1000 micrograms.ml-1) for 10 min, PMA-induced decrease of cytosol protein kinase C activity and increase of membrane protein kinase C activity were greatly inhibited in the same manner. These results suggest that PDS not only inhibited protein kinase C in vitro, but also inhibited activation of protein kinase C in cardiomyocytes.
Xu
X P
XP
Institute of Basic Medical Sciences, Chinese Academy Medical Sciences, Peking Union Medical College, Beijing.
Xiao
D M
DM
Zhou
W H
WH
Wang
X L
XL
Zhang
J B
JB
Shao
C J
CJ
Zhao
X J
XJ
Chen
H C
HC
D000818:Animals; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D036145:Ginsenosides; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D012503:Saponins / Q000494:pharmacology; D014315:Triterpenes / Q000494:pharmacology*
D036145:Ginsenosides; D010703:Phorbol Esters; D012503:Saponins; D014315:Triterpenes; C004564:panaxadiol; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C