[The assessment of the proliferative activity of bone marrow cells by flow cytofluorometry].
The distribution of bone marrow cells by the stages of cellular cycle (G1/0, S, G2 + M) was studied in 56 hematologically normal subjects and in 30 anemic patients by flow cytofluorometry. In health the analyzed parameters were found rather stable, well reproducible, this permitting their use as reference values. In pernicious anemia the S-phase fraction of bone marrow cells was found increased; a lesser increase was observed in the counts of myelokaryocytes in the postsynthetic phase of the mitotic cycle; in iron deficiency anemia these parameters virtually did not differ from the reference values, whereas in autoimmune anemia the pool of proliferating cells was increased. The author discusses the problems of the pathogenesis of anemias and the diagnostic value of his results.
Shmarov
D A
DA
D000744:Anemia, Hemolytic, Autoimmune / Q000473:pathology; D018798:Anemia, Iron-Deficiency / Q000473:pathology; D000752:Anemia, Pernicious / Q000473:pathology; D001707:Biopsy, Needle; D001854:Bone Marrow Cells; D002455:Cell Division; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D005456:Fluorescent Dyes; D006801:Humans; D012016:Reference Values
D005456:Fluorescent Dyes; D004247:DNA
Otsenka proliferativnoĭ aktivnosti kletok kostnogo mozga metodom protochnoĭ tsitofliuorometrii.