Sperm-immunobead binding decreases with in vitro incubation.
OBJECTIVE
To characterize the stability of the sperm-immunobead association over time.


METHODS
Prospective evaluation of sperm-immunobead binding, using direct and indirect assays.


METHODS
Male Infertility Clinic, University of California, Davis, Davis, California.


METHODS
Eleven men with sperm surface antibodies and 25 men with serum antisperm antibodies volunteered.


METHODS
Repeated assessment of sperm-immunobead binding over time.


RESULTS
Serum immunoglobulin (Ig)G decreased a mean of 42.6% over 30 minutes, and serum IgA decreased a mean of 22.7% over 30 minutes. Semen-derived IgG binding fell a mean of 59.9% and semen-derived IgA fell a mean of 27.0% over 25 to 40 minutes.


CONCLUSIONS
Sperm-immunobead coincubation results in a decrease in the number of sperm bound to immunobeads.
Gould
J E
JE
University of California, School of Medicine, Davis.
Brazil
C K
CK
Overstreet
J W
JW
D000906:Antibodies / Q000032:analysis*; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007158:Immunologic Techniques; D008297:Male; D008863:Microspheres; D011446:Prospective Studies; D013094:Spermatozoa / Q000276:immunology*; D013997:Time Factors
D000906:Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M