An EBV-based vector allowing a high level of LTRHIV-directed expression in human cells.
We have developed a vector that allows high and transactivable expression of inserted genes. The vector contains a transcription unit in which the LTR from HIV flanks a multicloning site. The plasmid is based on the EBV p205 plasmid, which allows stable replication in human cells. The ability of the vector to express an exogenous DNA in human cells has been tested using the firefly luciferase gene.
Raimond
J
J
Centre de Biophysique Moléculaire, Orléans, France.
Plessis
C
C
Fargette
F
F
Legrand
A
A
Hebert
E
E
D018076:DNA, Complementary / Q000235:genetics; D005057:Eukaryotic Cells / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors / Q000235:genetics*; D016325:HIV Long Terminal Repeat; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D014779:Virus Replication
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D008156:Luciferases