The sequence of porcine 80 kDa 17 beta-estradiol dehydrogenase reveals similarities to the short chain alcohol dehydrogenase family, to actin binding motifs and to sterol carrier protein 2.
The cDNA of porcine 17 beta-estradiol dehydrogenase codes for a polypeptide of 737 amino acids. The dehydrogenase activity of the 80 kDa translation product is located in its N-terminal 32 kDa fragment, which is the major form isolated from endometrial epithelium. beta-Actin co-purifies with some of the 32 kDa enzyme, which contains actin-binding motifs and is homologous to hydratase-dehydrogenase-epimerase of Candida tropicalis. The microbody-targeting signal AKI and sequences resembling sterol carrier protein 2 are present in the C-terminal part of the 80 kDa protein. The N- and C-terminal parts are connected by a sequence containing the putative protease recognition signal AAP.
Leenders
F
F
Max-Planck-Institut für experimentelle Endokrinologie, Hannover, Germany.
Husen
B
B
Thole
H H
HH
Adamski
J
J
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000737:chemistry; D000199:Actins / Q000378:metabolism*; D000426:Alcohol Dehydrogenase / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002175:Candida / Q000201:enzymology; D002352:Carrier Proteins / Q000737:chemistry*; D016384:Consensus Sequence; D004717:Endometrium / Q000201:enzymology; D004746:Enoyl-CoA Hydratase / Q000737:chemistry; D004960:Estradiol Dehydrogenases / Q000737:chemistry*; D005260:Female; D007535:Isomerases; D008830:Microbodies / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D063994:Peroxisomal Bifunctional Enzyme; D010940:Plant Proteins; D019998:Racemases and Epimerases / Q000737:chemistry; D017385:Sequence Homology; D013552:Swine
D000199:Actins; D002352:Carrier Proteins; D009097:Multienzyme Complexes; D010446:Peptide Fragments; D010940:Plant Proteins; C072087:sterol carrier proteins; D015094:3-Hydroxyacyl CoA Dehydrogenases; D000426:Alcohol Dehydrogenase; D004960:Estradiol Dehydrogenases; D004746:Enoyl-CoA Hydratase; D063994:Peroxisomal Bifunctional Enzyme; D007535:Isomerases; D019998:Racemases and Epimerases