Effect of bradykinin on loss of density-dependent growth inhibition of normal rat kidney cells.
Normal rat kidney fibroblasts, density-arrested in the presence of epidermal growth factor (EGF), can be restimulated to proliferate in a synchronous way and acquire a transformed phenotype following treatment with additional growth factors like retinoic acid (RA) and transforming growth factor (TGF)-beta. It was found that bradykinin has a strong inhibitory effect on growth stimulation induced by these factors, an effect which cannot be mimicked by PGF2 alpha. The growth-inhibiting effect can be blocked by inhibitors of cyclo-oxygenase activity, indicating that the relevant second messenger is most likely a prostaglandin. Externally added PGJ2, at a concentration of 10 microM, can mimic the inhibitory effect of bradykinin on the loss of density-arrest induced by RA suggesting that PGJ2 is a possible candidate for being the bradykinin induced growth-inhibiting prostaglandin.
Lahaye
D H
DH
Department of Cell Biology, University of Nijmegen, Faculty of Science, The Netherlands.
Afink
G B
GB
Bleijs
D A
DA
Van Alewijk
D C
DC
Van Zoelen
E J
EJ
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D015237:Dinoprost / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D007668:Kidney / Q000166:cytology / Q000187:drug effects*; D015230:Prostaglandin D2 / Q000031:analogs & derivatives / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology; D051381:Rats; D018002:Receptors, Bradykinin / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D016861:Cyclooxygenase Inhibitors; D011453:Prostaglandins; D018002:Receptors, Bradykinin; D016212:Transforming Growth Factor beta; D014212:Tretinoin; C037112:9-deoxy-delta-9-prostaglandin D2; D004815:Epidermal Growth Factor; D015237:Dinoprost; D015230:Prostaglandin D2; D001920:Bradykinin