[The development of a method for isolating and the study of the properties of an extract of the allergens from granary mites].
The method for obtaining allergenic extracts from T. putrescentiae bodies has been developed. As nutrient medium for the cultivation of these mites oat flour has been selected: it has no allergenic cross-reactive properties with T. putrescentiae. Some physico-chemical properties and specific activity of allergenic extracts obtained from these mites were studied. Protein content in these extracts has been found to vary from 0.93 to 0.26 mg/ml, depending on extraction conditions. Protein constitutes 1.5-30.0% of the dry substance of the allergen. After lyophilization the allergen becomes grayish-white powder, readily soluble in water and aqueous saline solutions. In addition, allergenic extracts from T. putrescentiae have pronounced capacity for binding specific IgE antibodies in the sera of patients sensitized to storage mites of this species, which has been confirmed by RAST inhibition and ELISA. Lyophilization produces no changes in the properties and activity of T. putrescentiae allergenic extracts.
Zheltikova
T M
TM
Mokronosova
M A
MA
Gervazieva
V B
VB
D000485:Allergens / Q000032:analysis / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002523:Edible Grain / Q000469:parasitology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D007158:Immunologic Techniques; D008925:Mites / Q000276:immunology*; D013997:Time Factors
D000485:Allergens; D007073:Immunoglobulin E
Razrabotka metoda polucheniia i izuchenie svoĭstv ékstrakta allergenov iz kleshcheĭ ambarno-zernovogo kompleksa.