Iminodipeptides containing proline with C-terminal and N-terminal residues prime the stimulation of human neutrophil superoxide generation by fMLP.
Preincubation of human peripheral blood polymophonuclear leukocytes with an iminodipeptide containing proline at the C-terminus and/or N-terminus, Pro-Pro, Gly-Pro, Pro-Gly, Ala-Pro and Pro-Ala, significantly enhanced N-formyl-methionyl-leucyl-phenylalanine-induced superoxide generation in a concentration-dependent manner. The iminodipeptide with C-terminal proline showed higher effect than that of the counterpart with N-terminal proline. These iminodipeptides also enhanced the superoxide generation induced by opsonized zymosan but not that induced by arachidonic acid or phorbol myristate acetate. Tyrosyl phosphorylation of the 45-kDa protein occurred in parallel with the iminodipeptide-dependent enhancement of superoxide generation in neutrophil.
Watanabe
Y
Y
Department of Medical Biology, Kochi Medical School, Japan.
Sagara
Y
Y
Sugahara
K
K
Kodama
H
H
D000595:Amino Acid Sequence; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011392:Proline / Q000032:analysis*; D013481:Superoxides / Q000378:metabolism*
D004151:Dipeptides; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D011392:Proline