Lectin-binding patterns in the development of the cerebellum.
We studied the binding distribution of several lectins, Con A, DBA, UEA and WGA, in the embryonic development of the chick cerebellum between stages 18 to 45 of Hamburger and Hamilton. We observed a differential labeling (in intensity and distribution) in the migratory and cortical layers of the cerebellum anlage with these different lectins. The different distributions and modifications in the labeling pattern suggest intense variations of the glycoproteins and glycosaminoglycans in the extracellular matrix during development. These variations coincide with cellular and organizational phenomena in the migratory and cortical layers, and suggest compartmentalization of the Purkinje cell labeling.
Viejo Tirado
F
F
Departamento de Ciencias Morfológicas I, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Peña Melián
A
A
Puerta Fonollá
J
J
D000818:Animals; D002531:Cerebellum / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism; D002642:Chick Embryo; D003208:Concanavalin A / Q000032:analysis; D005314:Embryonic and Fetal Development; D005109:Extracellular Matrix / Q000737:chemistry; D006023:Glycoproteins / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis; D037102:Lectins / Q000032:analysis / Q000378:metabolism*; D037121:Plant Lectins; D011485:Protein Binding; D014909:Wheat Germ Agglutinins / Q000032:analysis
D006023:Glycoproteins; D006025:Glycosaminoglycans; D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D014909:Wheat Germ Agglutinins; C036554:dolichos biflorus agglutinin; D003208:Concanavalin A