[The detection of GLUT2 gene mutation by polymerase-chain reaction single stranded conformation polymorphism (PCR-SSCP) method].
The polymerase chain reaction-single stranded conformation Polymorphism (PCR-SSCP) procedure was applied to examine whether the mutation in the liver/islet glucose transporter (GLUT2) gene could be associated with non-insulin-dependent diabetes mellitus (NIDDM) in Japanese. Samples were processed through 30-40 cycles of 1 min denaturation at 94 degrees C, 1 min annealing at 55-68 degrees C for 1-2 min, extention at 72 degrees C for 1 min, and denaturation at 94 degrees C for 1 min. We identified a silent mutation in codon 479 for PheTTT/TTC.
Miura
T
T
Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine.
Yamada
Y
Y
Someya
Y
Y
Kubota
A
A
Seino
Y
Y
D001483:Base Sequence; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic
D009004:Monosaccharide Transport Proteins