Identification of GPCMV infected cells in vitro and in vivo with a monoclonal antibody.
A monoclonal antibody was made which identifies a 160-180 kDa structural protein in guinea pig cytomegalovirus (GPCMV) infected cells by Western blot using non-reducing conditions. This protein was shown to be a virion structural protein by purification of GPCMV on a density viscosity gradient and Western blot analysis. Phosphoanacetic acid (PAA) experiments suggest that the protein is a late GPCMV protein. In vitro the monoclonal antibody labels a cytoplasmic protein in infected guinea pig embryo fibroblasts by 12 h postinfection. The monoclonal antibody also identifies GPCMV infected cells in vivo in paraffin embedded formalin fixed tissue.
Jones
C T
CT
Department of Pathology, University of Maryland, School of Medicine, Baltimore 21201.
Keay
S K
SK
Swoveland
P T
PT
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015153:Blotting, Western; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009500:Neutralization Tests; D012469:Salivary Glands / Q000821:virology; D015678:Viral Structural Proteins / Q000737:chemistry / Q000276:immunology; D014773:Virology / Q000379:methods*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015678:Viral Structural Proteins