title: [Identification of membrane proteins of sarcoplasmic reticulum binding 2,4,6-trinitrobenzene sulfonate].
abstract: Sarcoplasmic reticulum was treated with 2,4,6-trinitrobenzene sulfonate (TNBS), which binds covalently only with surface amino groups. After solubilization of the membranes and gel-filtration of the SR protein on the Sephadex G-100, the label was discovered in the protein fraction 100-80 and 70-60 kDa. The amino groups of proteins with molecular weight 50-20 kDa are inaccessible for the 2,4,6-TNBS. Incorporation of the latter into proteins increases in presence of KCl.
authors.forename: V A
authors.initials: VA
authors.lastname: Tugaĭ
authors.forename: V P
authors.initials: VP
authors.lastname: Zimina
mesh_terms: D005591:Chemical Fractionation; D002850:Chromatography, Gel; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D011485:Protein Binding; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D012995:Solubility; D014302:Trinitrobenzenesulfonic Acid / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D014302:Trinitrobenzenesulfonic Acid
vernacular_title: Identifikatsiia belkov membran sarkoplazmaticheskogo retikuluma, sviazyvasiushchikh 2,4,6-trinitrobenzolsul'fonat.