title: APS-sulfotransferase activity is identical to higher plant APS-kinase (EC 2.7.1.25).
abstract: A cDNA from Arabidopsis thaliana L. Heynh encoding the APS-kinase (EC 2.7.1.25) was modified by deletion of a plastidic transit peptide to enable its expression in Escherichia coli. The resultant protein (MW 25,761) is enzymatically active as APS-kinase and restores prototrophic growth in an APS-kinase mutant. All transformants harbouring the modified plant DNA also acquired APS-sulfotransferase activity. In the absence of ATP but provided with DTT, a tetrameric form of recombinant APS-kinase exhibits APS-sulfotransferase activity. Monospecific polyclonal antibodies raised against the APS-kinase as immunogen also reacted against APS-sulfotransferase. We propose that APS-sulfotransferase activity is a nonphysiological side reaction of APS-kinase.
authors.affiliation: Biochemistry of Plants, Ruhr University Bochum, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schiffmann
authors.forename: J D
authors.initials: JD
authors.lastname: Schwenn
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D003429:Cross Reactions; D004229:Dithiothreitol / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D017343:Genes, Plant; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018087:Plastids / Q000201:enzymology* / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D017384:Sequence Deletion; D015238:Sulfotransferases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D010446:Peptide Fragments; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C019617:adenylylsulfate kinase; D015238:Sulfotransferases; C081239:adenosine 5'-phosphosulfate sulfotransferase; D004229:Dithiothreitol