title: [Affinity modification of EcoRII restriction endonuclease by a DNA-duplex containing a monosubstituted pyrophosphate internucleotide bond].
abstract: Oligonucleotide duplex with an active monosubstituted pyrophosphate bond within the recognition site of the EcoRII restriction endonuclease was cross-linked to this enzyme with a yield of 10-15%. The cross-linking specificity was proved by the absence of the cross-linking to a DNA duplex with the same modification but without the EcoRII recognition site as well as by unmodified EcoRII substrate's inhibition of the cross-linking.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
authors.forename: G Ia
authors.initials: GIa
authors.lastname: Sheflian
authors.forename: S A
authors.initials: SA
authors.lastname: Kuznetsova
authors.forename: E A
authors.initials: EA
authors.lastname: Kubareva
authors.forename: O N
authors.initials: ON
authors.lastname: Sysoev
authors.forename: M G
authors.initials: MG
authors.lastname: Ivanovskaia
authors.forename: E S
authors.initials: ES
authors.lastname: Gromova
mesh_terms: D000345:Affinity Labels; D001483:Base Sequence; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry*; D011756:Diphosphates / Q000032:analysis*; D008969:Molecular Sequence Data
chemical_list: D000345:Affinity Labels; D003432:Cross-Linking Reagents; D011756:Diphosphates; D004247:DNA; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: Affinnaia modifikatsiia éndonukleazy restriktsii EcoRII DNA-dupleksom, soderzhashchim monozameshchennuiu pirofosfatnuiu mezhnukleotidnuiu sviaz'.