title: Biophysical characterization of the c-Myb DNA-binding domain.
abstract: We have examined proteins containing the DNA-binding domain of c-Myb with biophysical methods. This DNA-binding domain consists of three imperfect repeats (R1, R2, and R3) conserved among many species. Our results indicate that the DNA-binding domain forms unspecific and specific complexes with oligodeoxynucleotides. In the presence of R1, DNA sequences related to a canonical c-Myb-binding site are better discriminated. Furthermore, although R2 and R3 are sufficient for sequence-specific DNA binding, a structural change of the DNA-binding domain upon specific complex formation is induced only when R1 is present. Therefore, R1 might serve as an important element required for secondary structure alteration upon binding and its stabilization as well as for better discrimination between specific and related DNA sequences.
authors.affiliation: Zentrum Biochemie, Medizinische Hochschule Hannover, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Ebneth
authors.forename: O
authors.initials: O
authors.lastname: Schweers
authors.forename: H
authors.initials: H
authors.lastname: Thole
authors.forename: U
authors.initials: U
authors.lastname: Fagin
authors.forename: C
authors.initials: C
authors.lastname: Urbanke
authors.forename: G
authors.initials: G
authors.lastname: Maass
authors.forename: H
authors.initials: H
authors.lastname: Wolfes
mesh_terms: D001483:Base Sequence; D055592:Biophysical Phenomena; D001703:Biophysics; D002942:Circular Dichroism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D003852:Deoxyribonucleoproteins / Q000737:chemistry*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D017433:Protein Structure, Secondary; D011518:Proto-Oncogene Proteins / Q000737:chemistry*; D020598:Proto-Oncogene Proteins c-myb; D011994:Recombinant Proteins; D014357:Trypsin / Q000494:pharmacology; D014461:Ultracentrifugation
chemical_list: D004268:DNA-Binding Proteins; D003852:Deoxyribonucleoproteins; D009838:Oligodeoxyribonucleotides; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D011994:Recombinant Proteins; D014357:Trypsin