title: [The use of ELISA and indirect immunofluorescence technics for the rapid detection of eastern equine encephalomyelitis].
abstract: We present the results attained in the identification of Eastern equine encephalomyelitis virus isolations in Vero and XL-2 cell systems, using a double-antibody ELISA technique and the indirect immunofluorescence method. The results attained through these two techniques coincided by 100% with identification through neutralization. With the former, the virus was detected within 6-8 hours after inoculation. Better results were attained with XL-2 cells.
authors.affiliation: Instituto de Medicina Tropical Pedro Kourí.
authors.forename: J L
authors.initials: JL
authors.lastname: Pelegrino
authors.forename: S
authors.initials: S
authors.lastname: Vázquez
authors.forename: L
authors.initials: L
authors.lastname: Morier
authors.forename: A
authors.initials: A
authors.lastname: Castillo
authors.forename: M G
authors.initials: MG
authors.lastname: Guzmán
authors.forename: G
authors.initials: G
authors.lastname: Kourí
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004663:Encephalitis Virus, Eastern Equine / Q000276:immunology* / Q000302:isolation & purification; D004683:Encephalomyelitis, Equine / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D009500:Neutralization Tests / Q000662:veterinary; D013997:Time Factors
vernacular_title: Utilización de las técnicas ELISA e inmunofluorescencia indirecta para la detección rápida de la encefalomielitis equina del este.