title: Crystallization of enzyme IIB of the cellobiose-specific phosphotransferase system of Escherichia coli.
abstract: Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4 degrees. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 A resolution and have the monoclinic space group P2(1), with cell dimensions a = 53.6 A, b = 31.7 A, c = 60.0 A and beta = 101.7 degrees. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.
authors.affiliation: Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands.
authors.forename: R L
authors.initials: RL
authors.lastname: van Montfort
authors.forename: T
authors.initials: T
authors.lastname: Pijning
authors.forename: K H
authors.initials: KH
authors.lastname: Kalk
authors.forename: G K
authors.initials: GK
authors.lastname: Schuurman-Wolters
authors.forename: J
authors.initials: J
authors.lastname: Reizer
authors.forename: M H
authors.initials: MH
authors.lastname: Saier
authors.forename: G
authors.initials: G
authors.lastname: Robillard
authors.forename: B W
authors.initials: BW
authors.lastname: Dijkstra
mesh_terms: D002475:Cellobiose; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry*
chemical_list: D002475:Cellobiose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System