A two-site monoclonal antibody ELISA for the quantification of group V allergens in grass extracts.
A two-site solid-phase enzymimmunoassay was used for the quantification of group V allergen in grass pollen extracts in mass units. The assay is based on the monoclonal antibodies (MoAbs), 1D11 and 3B2 which recognize different epitopes on the standard Phl p V. The MoAb-ELISA is very sensitive (15-100 ng/ml Phl p V) and highly specific for group V allergens. Six pollen extracts of different grasses demonstrated parallel binding curves. The group V content ranged between 73 and 673 micrograms/ml in the extracts. The International Standard of Phleum pratense (IS 82/520) contains 400 micrograms/ml Phl p V. A good correlation was observed between the group V content and RAST inhibition, with the exception of Poa pratensis.
Fahlbusch
B
B
Department of Clinical Immunology, University of Jena, Germany.
Schlenvoigt
G
G
Müller
W D
WD
Weber
B
B
Jäger
L
L
D000485:Allergens / Q000032:analysis* / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D010936:Plant Extracts / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology*; D006109:Poaceae / Q000276:immunology*; D011058:Pollen / Q000276:immunology; D011852:Radioallergosorbent Test / Q000379:methods
D000485:Allergens; D000911:Antibodies, Monoclonal; C076049:Phl p V protein, Phleum pratense; D010936:Plant Extracts; D010940:Plant Proteins