Determination of flunitrazepam in plasma by liquid chromatography.
A reversed-phase liquid chromatographic method has been used to determine flunitrazepam in plasma. Extraction was simple and there was no need to hydrolyse the drug. Separation was achieved on a 150 x 3.9 mm i.d. column packed with 4-microns Nova Pack C18 using a mobile phase of water-acetonitrile-triethylamine (700:300:4, v/v/v) (adjusted to pH 7.5 with orthophosphoric acid). The method was shown to be rapid and reliable with a lower limit of detection of 5 ng ml-1. Results are reported of simple experiments on the effects of temperature and light on the stability of flunitrazepam in plasma kept on the laboratory bench.
Benhamou-Batut
F
F
Department of Clinical Pharmacology and Toxicology, CHU, Bordeaux, France.
Demotes-Mainard
F
F
Labat
L
L
Vinçon
G
G
Bannwarth
B
B
D002851:Chromatography, High Pressure Liquid; D005445:Flunitrazepam / Q000097:blood* / Q000506:poisoning; D006801:Humans; D013048:Specimen Handling; D013696:Temperature
D005445:Flunitrazepam