title: Adenovirus insertion encoding the Lac Z gene in human nervous cells in primary dissociated cultures.
abstract: Primary dissociated cultures of human fetal central nervous system cells were prepared and inoculated at different days in vitro with adenovirus that contained a reporter gene encoding beta-galactosidase. At various time intervals, the cultures were processed for characterization with X-gal histochemistry and additional immunostaining with neurofilament (NF), GABA and glial fibrillary acidic protein (GFA-P). We observed that NF (+) and GABA (+) neuronal as well as GFA-P (+) glial cells could express beta-galactosidase activity after inoculation. The labeling was detected up to 3 months after virus treatment. In addition, neurons cultivated for three months were found to be still permissive for virus infection. We can conclude that adenovirus may be considered as a potential vector to transfer genes to nerve cells.
authors.affiliation: INSERM U 336, Ecole Nationale Supérieure de Chimie, Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Levallois
authors.forename: A
authors.initials: A
authors.lastname: Privat
authors.forename: J
authors.initials: J
authors.lastname: Mallet
mesh_terms: D000260:Adenoviruses, Human / Q000201:enzymology / Q000235:genetics*; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000821:virology*; D018014:Gene Transfer Techniques; D005815:Genetic Code; D006801:Humans; D066298:In Vitro Techniques; D007763:Lac Operon / Q000235:genetics*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001616:beta-Galactosidase