[Study of the membrane expression of TcR/CD3 complex using somatic cell mutants].
T lymphocytes express membrane antigen receptor TcR/CD3 complexes only when all subunits are correctly assembled. Studies on TcR/CD3 membrane negative T cell variants containing all necessary subunits intracellularly, may allow to identify amino acids important for different subunit interactions. In this review, we summarize our recent work on TcR/CD3 negative variants of the human T cell line Jurkat. We found two critical amino acids in the TcR-alpha and TcR-beta extracellular constant regions (phenylalanine n. 216 and intrachain disulfide cysteine n. 212) involved in TcR-alpha beta/CD3- gamma epsilon, delta epsilon intermediary complex/zeta 2 homodimer interactions: (1) amino acid exchanges of phenylalanine demonstrated the importance of an aromatic amino acid residue at this position; (2) the intrachain disulfide bond assures a tertiary structure of the constant domain that is necessary for association with zeta 2 homodimers.
Caspar-Bauguil
S
S
Laboratoire d'Immunologie Cellulaire et Moléculaire, CRPG/CNRS, CHU Purpan, Toulouse, France.
Arnaud
J
J
Rubin
B
B
D000595:Amino Acid Sequence; D000818:Animals; D003545:Cysteine / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010649:Phenylalanine / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000096:biosynthesis* / Q000235:genetics; D016415:Sequence Alignment
D008565:Membrane Proteins; D017260:Receptor-CD3 Complex, Antigen, T-Cell; D010649:Phenylalanine; D003545:Cysteine
Etude de l'expression membranaire du complexe TcR/CD3 à l'aide de mutants cellulaires somatiques.