title: Detection of HPV DNA by in situ hybridization in benign, premalignant and malignant lesions of the oral mucosa.
abstract: Evidence has accumulated in recent years that relates certain types of human papillomaviruses (HPV) to the development of some tumors. We use in situ hybridization to study DNA from HPV 6/11, 16/18 and 31/33/35 in 6 squamous papillomas, 18 hyperkeratotic/acanthosis lesions with and without dysplasia (5 and 13 cases respectively) and 27 squamous cell carcinomas of the oral mucosa. HPV DNA was found in 66% of squamous papillomas, 38.4% of hyperkeratotic/acanthosis lesions without dysplasia, 60% of epithelial dysplasia and 37% of squamous cell carcinomas. HPV DNA 6/11 was the most common type found, and in squamous cell carcinomas HPV DNA appear more frequently in well differentiated tumors.
authors.affiliation: Department of Oral Medicine, School of Dentistry, University of Granada.
authors.forename: M A
authors.initials: MA
authors.lastname: González-Moles
authors.forename: I
authors.initials: I
authors.lastname: Ruiz-Avila
authors.forename: S
authors.initials: S
authors.lastname: González-Moles
authors.forename: I
authors.initials: I
authors.lastname: Martinez
authors.forename: A
authors.initials: A
authors.lastname: Ceballos
authors.forename: F
authors.initials: F
authors.lastname: Nogales
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000821:virology*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000235:genetics / Q000821:virology*; D009061:Mouth Mucosa / Q000821:virology; D009062:Mouth Neoplasms / Q000235:genetics / Q000821:virology*; D010212:Papilloma / Q000235:genetics / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics*; D011230:Precancerous Conditions / Q000235:genetics / Q000821:virology*; D011379:Prognosis
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral