[Isolation and purification of transcortin (cortisol binding globulin--CBG) using two-step chromatography].
The isolation of cortisol and progesterone binding globulin (CBG) from pregnant women serum was performed using affinity and hydrophobic chromatography. The purity and specificity of isolated transcortin was tested by agarose gel electrophoresis using racket and cross immunoelectrophoresis and specific CBG antibodies. High purity and immunoreactivity of the isolated globulin destitute of other proteins contamination, were obtained.
Głyda
A
A
Instytutu Ginekologii i Połoznictwa Akademii Medycznej w Poznaniu.
Kozak
W
W
Waśko
R
R
Horst-Sikorska
W
W
Sawicka
J
J
D002845:Chromatography / Q000379:methods*; D002846:Chromatography, Affinity; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D011247:Pregnancy / Q000097:blood*; D011376:Progesterone-Binding Globulin / Q000737:chemistry / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014156:Transcortin / Q000737:chemistry / Q000302:isolation & purification*
D011376:Progesterone-Binding Globulin; D014156:Transcortin
Izolacja i oczyszczanie transcortyny globuliny wiazacej kortyzol--(CBG)--za pomoca dwustopniowej chromatografii.