Formulation of a single-component kit for the preparation of technetium-99m labelled ethyl cysteinate dimer: biological and clinical evaluation.
Ethyl cysteinate dimer (ECD) labelled with reduced technetium-99m has recently been proposed as a promising radiopharmaceutical for brain perfusion imaging. In the present study a single-component kit formulation has been developed, thus simplifying the radiolabelling procedure. A method of analysis by electrophoresis has also been developed, permitting identification of radiochemical impurities in the preparation. 99mTc-ECD prepared by the single-component kit was further evaluated in primates and humans. The results demonstrated that the complex is stable in vivo, rapidly extracted and retained in the brain tissue for a sufficient time for single-photon emission tomography studies. Therefore the present single-component kit formulation can be proposed as a reliable instant freeze-dried kit for routine preparation of 99mTc-ECD required for scintigraphic assessment of regional cerebral blood flow.
Afshan
A
A
Nuclear Chemistry Division, Pakistan Institute of Nuclear Science and Technology, Islamabad.
Jehangir
M
M
Ashraf
M
M
Waqar
A
A
Chiotellis
E
E
D000328:Adult; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D002560:Cerebrovascular Circulation / Q000502:physiology; D003545:Cysteine / Q000031:analogs & derivatives*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D008875:Middle Aged; D015609:Organotechnetium Compounds; D011933:Reagent Kits, Diagnostic; D015899:Tomography, Emission-Computed, Single-Photon
D015609:Organotechnetium Compounds; D011933:Reagent Kits, Diagnostic; C059659:technetium Tc 99m bicisate; D003545:Cysteine