Transfer of shuttle vectors containing Bacillus thuringiensis toxin gene into wild-type B. cereus, B. brevis and B. subtilis by electroporation.
Gram positive and negative bacterial shuttle vectors carrying Bacillus thuringiensis (B.t.) toxin gene were introduced by electroporation into wild-type Bacillus cereus, B. brevis and B. subtilis. The transformation efficiencies for these bacteria were around 10(1)-10(4) transformants per micrograms DNA based on the numbers of neomycin- and ampicillin-resistant colonies produced. The structure of the transferred plasmids proved identical with the original ones both in size and restriction pattern. Toxicity assays showed that the transformants gave a mortality of 90-100% against caterpillar of Heliothis assulta, indicating that the gene function was not changed by electroporation.
Sun
L
L
Institute of Microbiology, Chinese Academy of Sciences, Beijing.
Liang
P
P
Tian
Y
Y
Mang
K
K
Ba
F
F
Ji
P
P
Mei
R
R
D001407:Bacillus / Q000235:genetics* / Q000254:growth & development; D001413:Bacillus thuringiensis / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D018274:Electroporation; D018014:Gene Transfer Techniques; D010957:Plasmids
D001427:Bacterial Toxins