title: Cloning and sequence analysis of the coat protein genes of an Australian strain of peanut mottle and an Indonesian 'blotch' strain of peanut stripe potyviruses.
abstract: We have analysed the coat protein gene sequences of two potyviruses infecting peanut. The 3' terminal 1247 nucleotides (nt) of an Australian strain of peanut mottle virus (PeMoV-AU) and the 3' terminal 1388 nt of an Indonesian 'blotch' strain of peanut stripe virus (PStV-Ib) were cloned and sequenced. Those regions included the 861 and 864 nt encoding the respective putative coat proteins as well as the 285 and 253 nt, respectively of 3' non-coding sequences. Comparison of the nucleotide sequences of PeMoV-AU and PStV-Ib revealed a sequence similarity of 64.4% for the coat protein gene and 34.6% for the 3' non-coding region. The deduced amino acid sequences of PeMoV-AU and PStV-Ib coat proteins are 66.7% identical. These results provide further evidence that PeMoV and PStV are distinct viruses. Comparisons of the 3' terminal sequences of PeMoV-AU and PStV-Ib with those of the genomic RNA of other strains of PeMoV and PStV and with other potyviruses are discussed.
authors.affiliation: Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Gehrmann Laboratories, University of Queensland, St. Lucia, Australia.
authors.forename: P Y
authors.initials: PY
authors.lastname: Teycheney
authors.forename: R G
authors.initials: RG
authors.lastname: Dietzgen
mesh_terms: D000595:Amino Acid Sequence; D010367:Arachis / Q000382:microbiology*; D001315:Australia; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry*; D003001:Cloning, Molecular; D007214:Indonesia; D008969:Molecular Sequence Data; D010802:Phylogeny; D017800:Potyvirus / Q000737:chemistry* / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics
chemical_list: D012367:RNA, Viral