title: PCR-cloning of goldfish and tilapia metallothionein complementary DNAs.
abstract: Metallothionein (MT) is believed to be a sensitive and effective "biomarker" for monitoring metal contamination in fish. Comparison of amino acid sequences between flounder MT and trout MT revealed that there is a conserved region at their N-terminals. Using oligonucleotides derived from this conserved region, reverse transcription- polymerase chain reaction (RT-PCR) was performed to obtain MT complementary DNAs (cDNAs) from goldfish, Carassius auratus, and tilapia, Tilapia mossambica. These cDNA probes would be useful in developing sensitive PCR-based methods to detect MT gene expression for monitoring metal pollution in local waters.
authors.affiliation: Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T.
authors.forename: K M
authors.initials: KM
authors.lastname: Chan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006054:Goldfish; D008668:Metallothionein / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D017210:Tilapia
chemical_list: D018076:DNA, Complementary; D008668:Metallothionein