On the mechanism of biotin synthase of Bacillus sphaericus.
A cell-free system of a bioB transformant of Bacillus sphaericus, effecting the last step of biotin biosynthesis, namely the introduction of sulfur into dethiobiotin has been recently described. S-adenosyl methionine (SAM) is absolutely necessary for activity. We show here, through experiments with [35S]SAM and [35S]Cys, that the sulfur donor is not SAM but probably cysteine (Cys) or a derivative. This finding together with the fact that NADPH and FAD are required for activity leads us to postulate some analogy between the biotin synthase system and other systems which use SAM as a source of desoxyadenosyl radical.
Florentin
D
D
Laboratoire de Chimie Organique Biologique, URA CNRS 493, Université Paris-VI, France.
Bui
B T
BT
Marquet
A
A
Ohshiro
T
T
Izumi
Y
Y
D001407:Bacillus / Q000201:enzymology*; D001710:Biotin / Q000096:biosynthesis*; D003545:Cysteine / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism; D013466:Sulfurtransferases / Q000378:metabolism*
D001710:Biotin; D012436:S-Adenosylmethionine; D013466:Sulfurtransferases; D003545:Cysteine