title: Comment: effect of cytokines on prolactin production by human decidual stromal cells in culture: studies using cells freed of bone marrow-derived contaminants.
abstract: Human decidua contains resident decidual cells alongside a population of bone marrow-derived cells, among which macrophages and large granular lymphocytes are most abundant. We hypothesized that soluble effectors produced by bone marrow-derived cells may modulate the function of the decidual cells. To investigate this, a cell purification protocol was devised that involved digestion of first-trimester decidua with collagenase and hyaluronidase to produce a mixed stromal cell suspension from which the bone marrow-derived cells were removed using immunomagnetic beads coated with anti-CD45. The resulting stromal cells were maintained in culture in the presence of progesterone and were found to produce PRL. The effect of a panel of cytokines on PRL production was examined. Tumor necrosis factors-alpha and -beta had a dose-dependent inhibitory effect, and tumor necrosis factor receptors were identified on the cells. Interleukin 1 alpha and 1 beta, platelet-derived growth factor, and transforming growth factor-beta 1 were also found to inhibit PRL production, and platelet-derived growth factor and transforming growth factor-beta 1 stimulated cell proliferation. These findings suggest an interaction between the immune and endocrine systems in regulating the maternal environment of early pregnancy.
authors.affiliation: INEP, University of Belgrade, Zemun, Yugoslavia.
authors.forename: L M
authors.initials: LM
authors.lastname: Vićovac
authors.forename: P M
authors.initials: PM
authors.lastname: Starkey
authors.forename: J D
authors.initials: JD
authors.lastname: Aplin
mesh_terms: D001854:Bone Marrow Cells; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D003656:Decidua / Q000378:metabolism*; D005260:Female; D006801:Humans; D018189:Immunomagnetic Separation; D007375:Interleukin-1 / Q000494:pharmacology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008214:Lymphocytes / Q000378:metabolism; D008233:Lymphotoxin-alpha / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D011388:Prolactin / Q000096:biosynthesis*; D017154:Stromal Cells / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D008233:Lymphotoxin-alpha; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D011374:Progesterone; D011388:Prolactin; D017493:Leukocyte Common Antigens