An evaluation of receptor-mediated gene transfer using synthetic DNA-ligand complexes.
Receptor-mediated gene transfer is an attractive method for therapeutically correcting human genetic diseases since it permits the targeting of DNA to cellular receptors in specific tissues of adult animals. Genes introduced by this technique have been shown to be expressed in the target tissue for varying periods. However, to be useful for gene therapy, it is critical that both the chemical properties and physical interactions of the reagents involved in the design of the DNA delivery vehicle be rigorously characterized. In this review, we discuss the critical steps in the preparation of the DNA-ligand complex and the factors involved in the delivery and regulated expression of a transgene in animal tissues. The feasibility of using this technique for the therapeutic delivery of genes to mammalian tissues will also be evaluated.
Perales
J C
JC
Department of Biochemistry, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4935.
Ferkol
T
T
Molas
M
M
Hanson
R W
RW
D000818:Animals; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D018014:Gene Transfer Techniques; D006801:Humans; D008024:Ligands; D011956:Receptors, Cell Surface
D008024:Ligands; D011956:Receptors, Cell Surface; D004247:DNA