Restriction endonuclease analysis of adenovirus isolates from sporadic and epidemic ocular infections: experience in a clinical laboratory.
Adenovirus isolates from 52 patients with ocular infection over a 3-year period were typed by restriction endonuclease analysis in a clinical laboratory. The results indicated that adenovirus type 8 was the most common cause of adenovirus eye infection during this period, being responsible for 42 (81%) of the 52 cases. Of 42 adenovirus type 8 isolates, 22 showed variant patterns by restriction endonuclease analysis and required multiple enzyme digests for identification. These isolates were readily identified by neutralisation tests.
Mahafzah
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A
Virology Reference Laboratory, Veterans Administration Medical Center, West Haven, CT.
Landry
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ML
D000258:Adenovirus Infections, Human / Q000382:microbiology*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics*; D003231:Conjunctivitis / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D015828:Eye Infections, Viral / Q000382:microbiology*; D006801:Humans; D007637:Keratoconjunctivitis / Q000382:microbiology; D009500:Neutralization Tests; D015183:Restriction Mapping
D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific