Immunorecognition of ring skeleton of taxanes by chicken egg yolk antibodies.
Anti-10 deacetylbaccatin III (DAB) antibodies (IgY) were elicited in hens immunized with a succinyl-DAB/BSA conjugate and extracted from egg yolk. As shown by indirect competitive inhibition enzyme immunoassay (CIEIA), the addition of free-DAB competitively inhibited the binding of affinity purified anti-DAB IgY to DAB/BSA solid phase conjugated antigen. The assay enabled the detection of DAB in concentrations as low as 7.5ng/ml (13.7 nM DAB), whereas anti-DAB IgY did not react with taxol even at a concentration a thousand times higher. The structural requirements of the diterpenoid nucleus for binding to IgY were considered on the basis of the levels of cross-reaction found with 10 authentic taxanes. The results indicate that anti-DAB IgY represents the first high affinity antibody produced capable of recognizing the ring skeleton of taxol precursors.
Concetti
A
A
Dipartimento di Biologia M.C.A., Camerino MC, Italy.
Ripani
E
E
Barboni
L
L
Torregiani
E
E
Bombardelli
E
E
Gariboldi
P
P
Venanzi
F M
FM
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D000918:Antibody Specificity; D001643:Bridged Bicyclo Compounds / Q000737:chemistry* / Q000276:immunology; D001952:Bridged-Ring Compounds; D002645:Chickens; D003429:Cross Reactions; D004530:Egg Yolk / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007118:Immunoassay; D018796:Immunoconjugates / Q000737:chemistry / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013386:Succinates / Q000737:chemistry / Q000276:immunology; D043823:Taxoids; D014315:Triterpenes / Q000276:immunology
D000906:Antibodies; D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D018796:Immunoconjugates; D013386:Succinates; D043823:Taxoids; D014315:Triterpenes; C080625:taxane; D012710:Serum Albumin, Bovine; C042213:10-deacetylbaccatine III