Isolation and characterization of a diuretic peptide common to the house fly and stable fly.
An identical CRF-related diuretic peptide (Musca-DP) was isolated and characterized from whole-body extracts of the house fly, Musca domestica, and stable fly, Stomoxys calcitrans. The peptide stimulates cyclic AMP production in Manduca sexta Malpighian tubules and increases the rate of fluid secretion by isolated Musca domestica tubules. The 44-residue peptide, with a mol.wt. of 5180, is amidated, and has the primary structure: NKPSLSIVNPLDVLRQRLLLEIARRQMKENTRQVELNRAILKNV-NH2. Musca-DP has a high percentage of sequence identity with other characterized CRF-related insect diuretic peptides.
Clottens
F L
FL
Food Animal Protection Research Laboratory, U.S. Department of Agriculture, College Station, TX 77845.
Holman
G M
GM
Coast
G M
GM
Totty
N F
NF
Hayes
T K
TK
Kay
I
I
Mallet
A I
AI
Wright
M S
MS
Chung
J S
JS
Truong
O
O
D000595:Amino Acid Sequence; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000031:analogs & derivatives*; D000242:Cyclic AMP / Q000378:metabolism; D004232:Diuretics / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006793:Houseflies / Q000737:chemistry; D008317:Malpighian Tubules / Q000187:drug effects; D018402:Manduca / Q000187:drug effects; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009117:Muscidae / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D004232:Diuretics; D010455:Peptides; D003346:Corticotropin-Releasing Hormone; D000242:Cyclic AMP