Separation of 2-arylpropionic acids on a cellulose based chiral stationary phase by RP-HPLC.
The enantiomers of eight 2-arylpropionic acids, a group of chiral non steroidal antiinflammatory drugs, were resolved as their benzylamide derivatives on a high-performance liquid chromatographic chiral stationary phase consisting of a covalently bound tris (4-methylbenzoate) cellulose layer on silica gel. The column was used under reversed-phase conditions using methanol as the main mobile phase component, with a perchlorate buffer pH 2.0. A compromise for derivatization with a water soluble carbodiimide and 1-hydroxybenzotriazole of a group of eight analytes was obtained. The derivatives were identified by IR- and MS-spectroscopy.
Van Overbeke
A
A
Laboratory of Drug Analysis, Faculty of Pharmaceutical Sciences, University of Ghent, Belgium.
Baeyens
W
W
Van den Bossche
W
W
Dewaele
C
C
D000577:Amides / Q000737:chemistry / Q000302:isolation & purification; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification*; D001565:Benzoates; D002482:Cellulose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D000432:Methanol; D010666:Phenylpropionates / Q000302:isolation & purification*; D058428:Silica Gel; D012822:Silicon Dioxide; D013055:Spectrophotometry, Infrared; D013237:Stereoisomerism
D000577:Amides; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D010666:Phenylpropionates; C090722:tris(4-methylbenzoate)cellulose; D058428:Silica Gel; D012822:Silicon Dioxide; D002482:Cellulose; D000432:Methanol