Inhibitory effects of sho-seiryu-to on acetylcholine-induced responses in nasal gland acinar cells.
Sho-seiryu-to, a traditional Japanese herbal medicine, has been used extensively in the treatment of allergic rhinitis. The effects of Sho-seiryu-to on electrical responses induced by acetylcholine in dissociated nasal gland acinar cells were investigated using patch-clamp and microfluorimetric imaging techniques. The application of Sho-seiryu-to inhibited both K+ and Cl- currents augmented by acetylcholine. The elevation of intracellular Ca2+ and Na+ concentrations induced by acetylcholine was also inhibited by Sho-seriyu-to. These findings suggest that Sho-seiryu-to attenuated the secretion of water and electrolytes from the nasal glands through an anti-cholinergic effect.
Ikeda
K
K
Department of Otolaryngology, Tohoku University School of Medicine, Sendai, Japan.
Wu
D Z
DZ
Ishigaki
M
M
Sunose
H
H
Takasaka
T
T
D000109:Acetylcholine / Q000633:toxicity; D000818:Animals; D002118:Calcium / Q000378:metabolism; D018118:Chloride Channels / Q000187:drug effects / Q000502:physiology; D003600:Cytosol / Q000378:metabolism; D004195:Disease Models, Animal; D004357:Drug Synergism; D005470:Fluorometry; D006168:Guinea Pigs; D007564:Japan; D008518:Medicine, East Asian Traditional; D008564:Membrane Potentials / Q000187:drug effects; D009300:Nasal Septum / Q000166:cytology / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy; D012964:Sodium / Q000378:metabolism
D018118:Chloride Channels; D010936:Plant Extracts; D015221:Potassium Channels; D012964:Sodium; D000109:Acetylcholine; D002118:Calcium