title: Lack of promoting effect of proline on bile duct cancer development in dimethylnitrosamine-initiated hamster livers.
abstract: Bile duct hyperplasia caused by proline is believed to represent a chemical effect of the liver fluke, Fasciola hepatica, and the resultant cell division might be expected to play a role as a tumor promoter. To investigate the potential promoting effect of proline on bile duct cancer development, Syrian hamsters were therefore divided into 8 treatment groups: dimethylnitrosamine (DMN) + proline intraperitoneally (i.p.); DMN + proline s.c.; DMN + saline i.p.; DMN + saline s.c.; proline i.p.; proline s.c.; saline i.p.; and saline s.c. DMN was injected i.p. at 20 mg/kg to the animals 2 weeks prior to commencement of proline treatment, whereby 1 ml of a 2 M solution was given by i.p. or s.c. injection 3 times a week for 20 weeks. At the end of week 42, assessment of preneoplastic lesion development did not reveal any significant modulating influence of proline on DMN-initiated lesion development nor did it itself cause persistent bile duct hyperplasia.
authors.affiliation: Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok.
authors.forename: W
authors.initials: W
authors.lastname: Thamavit
authors.forename: C
authors.initials: C
authors.lastname: Pairojkul
authors.forename: D
authors.initials: D
authors.lastname: Tiwawech
authors.forename: T
authors.initials: T
authors.lastname: Shirai
authors.forename: N
authors.initials: N
authors.lastname: Ito
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms / Q000139:chemically induced*; D001652:Bile Ducts / Q000187:drug effects / Q000473:pathology; D006224:Cricetinae; D004128:Dimethylnitrosamine; D006965:Hyperplasia; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced*; D008297:Male; D008647:Mesocricetus; D011230:Precancerous Conditions / Q000139:chemically induced*; D011392:Proline / Q000633:toxicity*
chemical_list: D011392:Proline; D004128:Dimethylnitrosamine