title: Density gradient separation of L-asparaginase-loaded human erythrocytes.
abstract: L-Asparaginase has been encapsulated in human red blood cells using a hypotonic dialysis process. Erythrocytes loaded with L-Asparaginase were separated into eight fractions using a discontinuous Percoll density gradient. A minor cell subpopulation of low density cells and a major subpopulation of denser erythrocytes was obtained after hypotonic dialysis treatment, in both the absence or presence of L-Asparaginase. The encapsulated L-Asparaginase activity per resealed erythrocyte was higher in low-density cells and decreased progressively with increasing in cellular density.
authors.affiliation: Laboratoire de Biopharmacologie Transfusionnelle, Centre Régional de Transfusion Sanguine, Tours, France.
authors.forename: M I
authors.initials: MI
authors.lastname: Garín
authors.forename: R
authors.initials: R
authors.lastname: Kravtzoff
authors.forename: N
authors.initials: N
authors.lastname: Chestier
authors.forename: S
authors.initials: S
authors.lastname: Sanz
authors.forename: M
authors.initials: M
authors.lastname: Pinilla
authors.forename: J
authors.initials: J
authors.lastname: Luque
authors.forename: C
authors.initials: C
authors.lastname: Ropars
mesh_terms: D001215:Asparaginase; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003956:Dialysis; D004339:Drug Compounding / Q000379:methods*; D004912:Erythrocytes; D006801:Humans; D007038:Hypotonic Solutions; D011205:Povidone; D012822:Silicon Dioxide
chemical_list: D007038:Hypotonic Solutions; C016039:Percoll; D012822:Silicon Dioxide; D001215:Asparaginase; D011205:Povidone