title: Microbial hydrolysis of glutaronitrile derivatives with Brevibacterium sp. R 312.
abstract: The enantiomerically pure (S)-cyano acids 3 and 4 can be obtained by biotransformation with Brevibacterium sp. R 312 of the corresponding prochiral dinitriles 5 and 6, respectively. The hydrolysis is probably a two step process involving a nitrile hydratase and an amidase. In connection with these investigations a facile method for the synthesis of racemic 4-cyano-3-hydroxybutanoic acid derivatives was developed.
authors.affiliation: Department of Biological Sciences, University of Exeter, Devon, U.K.
authors.forename: A
authors.initials: A
authors.lastname: Kerridge
authors.forename: J S
authors.initials: JS
authors.lastname: Parratt
authors.forename: S M
authors.initials: SM
authors.lastname: Roberts
authors.forename: F
authors.initials: F
authors.lastname: Theil
authors.forename: N J
authors.initials: NJ
authors.lastname: Turner
authors.forename: A J
authors.initials: AJ
authors.lastname: Willetts
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D001555:Benzene Derivatives; D001711:Biotransformation; D001951:Brevibacterium / Q000254:growth & development / Q000378:metabolism*; D002087:Butyrates / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D015394:Molecular Structure; D009570:Nitriles / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013237:Stereoisomerism
chemical_list: C091063:3-benzyloxy-4-cyanobutanoic acid; D001555:Benzene Derivatives; D002087:Butyrates; D009570:Nitriles; C091062:3-benzoyloxy-4-cyanobutanoic acid; D000581:Amidohydrolases; D006836:Hydro-Lyases; C048831:nitrile hydratase