title: Biodynamic study of americium-241 accumulation in the cytosol of the hepatopancreas of the lobster Homarus gammarus.
abstract: In the lobster, most of the radionuclides ingested with contaminated food are concentrated in the digestive gland. Americium-241 accumulation in the hepatopancreas of the lobster was studied during the digestive cycle. Fractionations of cytosols at different times after ingestion of radioactive preys were performed by gel permeation chromatography to determine the distribution of 241Am in the different macromolecular components. 241Am was associated with ferritin during the whole digestive cycle. This observation suggests a correlation between 241Am distribution pathways and iron metabolism. The distribution of 241Am present in the other cytosolic proteins followed two major steps of accumulation which may be correlated to the evolution of the two main cellular types playing an important role in the digestive cycle (B and R type cells).
authors.affiliation: Laboratoire de Biochimie et de Radiobiochimie, GERMETRAD, Université de Nantes, France.
authors.forename: F
authors.initials: F
authors.lastname: Goudard
authors.forename: F
authors.initials: F
authors.lastname: Paquet
authors.forename: J P
authors.initials: JP
authors.lastname: Durand
authors.forename: M C
authors.initials: MC
authors.lastname: Milcent
authors.forename: P
authors.initials: P
authors.lastname: Germain
authors.forename: J
authors.initials: J
authors.lastname: Pieri
mesh_terms: D000576:Americium / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D004063:Digestion; D005293:Ferritins / Q000378:metabolism; D008024:Ligands; D008099:Liver / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism; D008121:Nephropidae / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism; D014018:Tissue Distribution; D014875:Water Pollutants, Radioactive / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity
chemical_list: D002352:Carrier Proteins; D008024:Ligands; D014875:Water Pollutants, Radioactive; D005293:Ferritins; D008668:Metallothionein; D000576:Americium