Construction of recombinant infectious laryngotracheitis virus expressing the LacZ gene of E. coli with thymidine kinase gene.
We constructed the recombinant infectious laryngotracheitis virus (ILTV), CE strain, containing the LacZ gene of E. coli in the thymidine kinase gene. The growth property of the recombinant virus was almost the same as parental CE strain in chicken embryo fibroblasts. The recombinant CE strain of ILTV could be used as a live vaccine vector.
Okamura
H
H
Chemo-Sero Therapeutic Research Institute, Kumamoto, Japan.
Sakaguchi
M
M
Honda
T
T
Taneno
A
A
Matsuo
K
K
Yamada
S
S
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005814:Genes, Viral; D005819:Genetic Markers; D005822:Genetic Vectors; D001352:Herpesvirus 1, Gallid / Q000235:genetics* / Q000254:growth & development; D007763:Lac Operon; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D013937:Thymidine Kinase / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers; D013937:Thymidine Kinase