Structure and solution properties of lipidheme-microsphere.
Triglyceride microsphere emulsified with phospholipid derivative of heme (5,10,15,20-tetrakis[alpha,alpha,alpha,alpha-o-[2,2-dimethyl-20- [2-(trimethylammonioethoxy) phosphonatoxy]eicosanamido]phenyl]porphinatoiron(II); lipidheme) provides a totally synthetic artificial red cell (lipidheme-microsphere; LH-M). Its structure, solution properties and O2 binding ability are described. The particle diameter of the LH-M was ca. 90 nmø elucidated by electron microscopy. Viscosity of the LH-M suspension (approximately 1.5 cP) was much lower than that of human blood and the viscosity of mixed system of LH-M/human blood (1/1(v/v)) was 2.5 cP. Specific gravity, osmotic pressure, and colloid osmotic pressure of the LH-M suspension also satisfied the physiological needs. The LH-M can bind O2 reversibly in response to O2 pressure (P50(O2): 41 torr (pH 7.4, 37 degrees C)). O2 solubility of the LH-M was more than that of human blood caused by its high heme concentration.
Komatsu
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T
Department of Polymer Chemistry Waseda University, Tokyo, Japan.
Nishide
H
H
Tsuchida
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E
D001802:Blood Substitutes / Q000737:chemistry* / Q000378:metabolism; D006418:Heme / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes; D008854:Microscopy, Electron; D008863:Microspheres; D015394:Molecular Structure; D010100:Oxygen / Q000378:metabolism; D010316:Particle Size; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D012996:Solutions; D014783:Viscosity
D001802:Blood Substitutes; D008055:Lipids; D008081:Liposomes; D011108:Polymers; D012996:Solutions; D006418:Heme; D010100:Oxygen