[Low affinity human IgE receptor: cloning cDNA from B-lymphocytes of cell line 1B and study of its expression in peripheral blood B-cells].
Previously, the cDNA coding for human low-affinity receptor for IgE (FcERII/CD23) was cloned by three independent groups. Using oligonucleotide probes corresponding to the published cDNA sequence of CD23, we have isolated cDNA clones encoding FcERII from the cDNA library of B-lymphoblastoid cell line 1B obtained in our laboratory. Cloned nucleotide sequences of FcERII were shown to be identical to those from the RPMI8866 cell line. Using a DNA fragment containing the full-length cDNA copy of CD23 as a probe, CD23 expression in highly purified peripheral blood B lymphocytes was shown to be induced by recombinant human IL4 in a clearly dose-dependent manner.
Pivniuk
V I
VI
Chelidze
L K
LK
Martsen
E O
EO
Tsytsikov
E N
EN
Vasilov
R G
RG
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D008969:Molecular Sequence Data; D017455:Receptors, IgE / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000187:drug effects
D018076:DNA, Complementary; D017455:Receptors, IgE; D011994:Recombinant Proteins; D015847:Interleukin-4
Nizkoaffinnyĭ retseptor IgE cheloveka: klonirovanie kDNK iz B-limfoblastoidnoĭ kletochnoĭ linii 1B i izychenie ego ékspressii v B-kletkakh perifericheskoĭ krovi.