Pancreatic beta-cell secretory defect associated with mitochondrial point mutation of the tRNA(LEU(UUR)) gene: a study in seven families with mitochondrial encephalomyopathy, lactic acidosis and stroke-like episodes (MELAS).
Recent evidence suggests possible linkage between diabetes mellitus and mitochondrial gene mutation. We surveyed mitochondrial tRNA(LEU(UUR)) (3243) mutation in 7 mitochondrial encephalomyopathy, lactic acidosis and stroke-like episode (MELAS) families and identified 24 mutated subjects (7 MELAS probands and 17 non-MELAS relatives) as well as 11 non-mutant family members. An OGTT in the 24 mutant relatives revealed 14 diabetic subjects, 3 with impaired glucose tolerance and 7 with normal glucose tolerance and all non-mutant family members as having normal glucose tolerance. Insulinogenic index was significantly reduced in the mutant diabetic subjects and those with impaired and normal glucose tolerance in comparison with the normal control subjects and the non-mutant members. Urinary 24-h C-peptide immunoreactivity excretion was markedly reduced in the mutant diabetic subjects and those with normal and impaired glucose tolerance, compared with the control subjects and the non-mutant family members. Plasma C-peptide immunoreactivity 6 min after glucagon injection was markedly reduced in the mutant diabetic subjects and those with normal and impaired glucose tolerance compared with the control subjects and the non-mutant family members. Si, an index of insulin sensitivity of the four mutant subjects was within normal range. Islet cell antibodies were negative in sera of eight mutated diabetic subjects, 2 and 6 with impaired and normal glucose tolerance, respectively. Diabetic retinopathy and nephropathy were demonstrated in 7 (50%) and 12 (85.7%) of 14 mutant diabetic subjects, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Suzuki
S
S
Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Hinokio
Y
Y
Hirai
S
S
Onoda
M
M
Matsumoto
M
M
Ohtomo
M
M
Kawasaki
H
H
Satoh
Y
Y
Akai
H
H
Abe
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D002096:C-Peptide / Q000652:urine; D002648:Child; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D003920:Diabetes Mellitus / Q000235:genetics* / Q000652:urine; D005260:Female; D006801:Humans; D007515:Islets of Langerhans / Q000502:physiology / Q000503:physiopathology*; D017241:MELAS Syndrome / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D010375:Pedigree; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012356:RNA, Transfer, Leu / Q000235:genetics*; D012016:Reference Values
D002096:C-Peptide; D017931:DNA Primers; D004272:DNA, Mitochondrial; D012356:RNA, Transfer, Leu