Primary retroperitoneal mucinous cystoadenocarcinomas: an immunohistochemical and molecular study.
Special immunohistochemical stains for the identification of gastroenteropancreatic antigens in two cases of primary retroperitoneal mucinous cystoadenocarcinomas (PRMC) show that these tumours have patterns similar to ovarian mucinous tumours. Markers of pyloric type gastric mucosa differentiation (M1, cathepsin E, concavavalin A, pepsinogen II) are mostly positive in benign and borderline areas with endocervical type differentiation, while immunoreactivity for intestinal cell markers (M3SI and CAR-5) and for DU-PAN-2 is present mainly in frankly malignant areas, regardless of differentiation type. DNA analysis shows a point mutation of K-ras oncogene at codon 12 (GGT to CGT) in one case. The immunohistochemical and genotypic similarity of PRMC and ovarian mucinous tumours may indicate similar mechanisms in their histogenesis.
Tenti
P
P
Department of Pathology, University of Pavia, Italy.
Romagnoli
S
S
Pellegata
N S
NS
Zappatore
R
R
Giunta
P
P
Ranzani
G N
GN
Carnevali
L
L
D000943:Antigens, Differentiation / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D020059:Cathepsin E; D002403:Cathepsins / Q000032:analysis; D003208:Concanavalin A / Q000032:analysis; D018282:Cystadenocarcinoma, Mucinous / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D004247:DNA / Q000032:analysis; D005260:Female; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009077:Mucins; D009154:Mutation; D010435:Pepsinogens / Q000032:analysis; D012186:Retroperitoneal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology
D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; C042317:DU-PAN-2 antigen, human; D009077:Mucins; D010435:Pepsinogens; C042384:gastro-intestinal mucus-associated antigens; D003208:Concanavalin A; D004247:DNA; D002403:Cathepsins; D020059:Cathepsin E