Translocation of protein kinase C alpha and zeta in rat glomerular mesangial cells cultured under high glucose conditions.
The activities and expression of protein kinase C isoenzymes were examined in glomerular mesangial cells cultured under high glucose conditions. Exposure of cells to high glucose concentrations (27.8 mmol/l) for more than 3 days resulted in a significant elevation of protein kinase C activities in the membrane fraction. Of the protein kinase C isoenzymes, the levels of protein kinase C alpha significantly increased in the membrane fraction after 3 days of exposure to glucose, and protein kinase C zeta increased after 5 days of exposure. Levels of protein kinase C delta and epsilon remained unchanged and protein kinase, C beta and gamma were not detected. These results indicate that protein kinase C alpha and zeta are translocated under high glucose conditions possibly through different mechanisms.
Kikkawa
R
R
Third Department of Medicine, Shiga University of Medical Science, Japan.
Haneda
M
M
Uzu
T
T
Koya
D
D
Sugimoto
T
T
Shigeta
Y
Y
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D015870:Gene Expression; D005920:Glomerular Mesangium / Q000187:drug effects / Q000201:enzymology*; D005947:Glucose / Q000494:pharmacology*; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D007527:Isoenzymes; D011493:Protein Kinase C; D005947:Glucose