A new reliable method of culturing and measuring human decidual capillary endothelial cells.
A reliable method of culturing human decidual capillary endothelial cells was developed. Endothelial growth cell supplement, heparin and newborn bovine serum were added to the culture medium to facilitate growth. Decidual capillary endothelial cell cultures showed similar growth curves to the more traditional human umbilical cord vein endothelial cell cultures. Culture growth was assessed by a new morphometric method of measuring cell culture area with the use of a computerised measuring tablet. This new method was compared with the more laborious method of counting individual cells and was found to be as reliable and more efficient.
Peek
M J
MJ
Department of Obstetrics and Gynaecology, University of Sydney, New South Wales, Australia.
Fraser
I S
IS
Johannisson
E
E
Markham
R
R
D002455:Cell Division; D002478:Cells, Cultured / Q000166:cytology*; D003656:Decidua / Q000098:blood supply*; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D011247:Pregnancy; D015203:Reproducibility of Results; D014841:von Willebrand Factor / Q000032:analysis
D014841:von Willebrand Factor