title: A myosin from a higher plant has structural similarities to class V myosins.
abstract: In plant cells, myosin is believed to be the molecular motor responsible for actin-based motility processes such as cytoplasmic streaming and directed vesicle transport. In an effort to characterize plant myosin, a cDNA encoding a myosin heavy chain was isolated from Arabidopsis thaliana. The predicted product of the MYA1 gene is 173 kDa and is structurally similar to the class V myosins. It is composed of the highly-conserved NH2-terminal "head" domain, a putative calmodulin-binding "neck" domain, an alpha-helical coiled-coil domain, and a COOH-terminal domain. Northern blot analysis shows that the Arabidopsis MYA1 gene is expressed in all the major plant tissues (flower, leaf, root, and stem). We suggest that the MYA1 myosin may be involved in a general intracellular transport process in plant cells.
authors.affiliation: Department of Biology, University of Michigan, Ann Arbor 48109.
authors.forename: M
authors.initials: M
authors.lastname: Kinkema
authors.forename: J
authors.initials: J
authors.lastname: Schiefelbein
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009218:Myosins / Q000737:chemistry / Q000235:genetics*; D011487:Protein Conformation; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; C087819:MYA1 protein, Arabidopsis; D012333:RNA, Messenger; D009218:Myosins