Interleukin 1 activity in haemolymph from strains of the snail Biomphalaria glabrata varying in susceptibility to the human blood fluke, Schistosoma mansoni: presence, differential expression, and biological function.
Cytokines control many of the steps in the complex pathways of immune and inflammatory responses in mammals. Recent reports also indicate that some invertebrates may produce cytokines such as interleukin 1 (IL-1). Certain strains of the snail, Biomphalaria glabrata (intermediate host for the human blood fluke, Schistosoma mansoni), possess a soluble plasma factor that stimulates the haemocyte-mediated killing of larval schistosomes, making them resistant to infection. In this study, we have sought to determine whether these snails possessed IL-1 in their plasma, and whether this cytokine was associated with resistance of B. glabrata to S. mansoni. Plasma from susceptible (M-line) and resistant (10-R2, 13-16-R1) strains of B. glabrata that had been unexposed or exposed to S. mansoni were tested for the presence of IL-1-like activity. Experiments employing both a bioassay and an immunoassay indicated that an IL-1-like molecule was present, in varying quantities, among the snail strains. Further, plasma IL-1 levels were significantly affected by exposure to S. mansoni, with levels dropping in M-line and 10-R2 snails, but increasing in the 13-16-R1 strain. However, both resistant strains maintained significantly higher IL-1 levels than M-line snails. Recombinant, human IL-1 (rhIL-1) was shown to prime haemocytes from both resistant snail strains for superoxide production, but had no effect on haemocytes from susceptible B. glabrata. Moreover, the addition of an IL-1 receptor antagonist protein (IRAP) eliminated this priming effect. Priming with rhIL-1 and/or IRAP had no effect on phagocytosis rates in any of the snail strains tested.(ABSTRACT TRUNCATED AT 250 WORDS)
Granath
W O
WO
Division of Biological Sciences, University of Montana, Missoula 59812.
Connors
V A
VA
Tarleton
R L
RL
D000818:Animals; D001681:Biological Assay; D001700:Biomphalaria / Q000276:immunology* / Q000469:parasitology*; D002460:Cell Line; D004198:Disease Susceptibility; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006434:Hemocytes / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D051379:Mice; D010587:Phagocytosis; D012550:Schistosoma mansoni / Q000472:pathogenicity*; D013481:Superoxides / Q000378:metabolism
D007375:Interleukin-1; D013481:Superoxides