Effect of the inoculum size on susceptibility tests performed on sessily growing bacteria.
It has been clearly established that the inoculum size greatly affects the results of antibiotic susceptibility tests performed in both liquid and solid media in standard laboratory growth conditions (i.e. planktonic). Recently methods were developed to perform antibiotic susceptibility tests on bacteria growing in sessile conditions. The present study investigates the effect of the inoculum size on results obtained by these methods. Results show that the inoculum size does not affect tests performed in sessile conditions. A simple and reliable method is proposed to be applied to routine microbiological laboratory procedures.
Selan
L
L
Cattedra di Microbiologia ed Igiene, Facoltà di Farmacia, Università La Sapienza, Rome, Italy.
Passariello
C
C
Berlutti
F
F
Thaller
M C
MC
Renzini
G
G
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development*; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000254:growth & development*
D000900:Anti-Bacterial Agents