Purification of tropomyosin from bovine adrenal medulla and its inhibitory effect on the actin severing activity of adseverin (adrenal medulla 74 kDa actin severing protein).
Tropomyosin was purified from a gelation product produced in the crude extract of bovine adrenal medulla using several column chromatographies mixed with a boiling treatment. Purified tropomyosin was a mixture of major isoforms with low molecular weights and minor isoforms of high molecular weights. Purified tropomyosin showed a dose-dependent protective effect on actin filaments against the severing activity of adseverin derived from adrenal medulla, suggesting its important role in the regulation of cell shape and structure in vivo.
Maekawa
S
S
Graduate School, Division of Science, University of Tokyo, Japan.
Toriyama
M
M
D008841:Actin Cytoskeleton / Q000187:drug effects; D000199:Actins / Q000378:metabolism*; D000313:Adrenal Medulla / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D018260:Gelsolin / Q000037:antagonists & inhibitors / Q000378:metabolism; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008970:Molecular Weight; D014335:Tropomyosin / Q000302:isolation & purification*
D000199:Actins; D018260:Gelsolin; D008840:Microfilament Proteins; D014335:Tropomyosin; C065395:scinderin; D002118:Calcium