Automated determination of midazolam in human plasma by high-performance liquid chromatography using column switching.
An automated gradient high-performance liquid chromatographic method using a column-switching technique was developed in order to determine and quantify midazolam (separated from the metabolite alpha-hydroximidazolam) in human plasma. After dilution with an internal standard (flurazepam) solution, containing 20% acetonitrile, 400 microliters of the plasma samples were injected onto a precolumn (17 x 4.6 mm I.D., C18 Corasil 37-53 microns) and retained. Proteins and polar plasma components were washed out using a 0.1 M sodium hydroxide solution, followed by an equilibration with a phosphate buffer of pH 8.0. After column-switching midazolam and flurazepam were eluted and transferred to the analytical column (RP-select B) in the backflush mode, separated by gradient elution and detected at 230 nm by ultraviolet detection. Precision of replicate analyses on the same day was 1.5% for midazolam and 0.7% for flurazepam. Recovery of midazolam was in the range 80-89% and the detection limit was 2 ng/ml plasma.
Lauber
R
R
Institute for Anaesthesiology and Intensive Care, University Hospital, Bern, Switzerland.
Mosimann
M
M
Bührer
M
M
Zbinden
A M
AM
D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008874:Midazolam / Q000097:blood*; D012680:Sensitivity and Specificity
D008874:Midazolam