A microplate-based bioassay system for measuring porcine serum mitogenic activity.
A bioassay system was developed to measure porcine serum mitogenic activity, which is an indicator of growth factor activity. A MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide++ +] cell proliferation assay was used to monitor cell growth. In comparison with electronic cell counting, this bioassay system is sensitive, economical, and semi-automatic. A non-fusing myoblast cell line, BC3H1, was used, making it possible to increase seeding density and thus improve precision. The assay was carried out in 96-well microplates with automatic reading and data processing by a computer-controlled ELISA reader. Results were highly correlated with Kotts' method. This assay system is of potential value in studying growth regulation in pigs.
Zhou
W
W
Department of Animal Science, Virginia Polytechnic Institute and State University, Blacksburg 24061-0306.
Kornegay
E T
ET
Storrie
B
B
D000818:Animals; D001681:Biological Assay / Q000662:veterinary*; D002452:Cell Count / Q000662:veterinary; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells; D004396:Coloring Agents; D003402:Creatine Kinase / Q000378:metabolism; D008934:Mitogens / Q000097:blood*; D018482:Muscle, Skeletal / Q000166:cytology; D009130:Muscle, Smooth / Q000166:cytology; D013552:Swine / Q000097:blood*; D013778:Tetrazolium Salts; D013844:Thiazoles
D004396:Coloring Agents; D008934:Mitogens; D013778:Tetrazolium Salts; D013844:Thiazoles; D003402:Creatine Kinase; C022616:thiazolyl blue