Chimerism in PCR products from a multigene family.
This paper describes a potential hazard in obtaining sequences of a multigene family by amplifying the mixed population by the polymerase chain reaction with primers from conserved sequences. We describe a product which appears to be a chimera formed during the PCR reaction from two different but related sequences.
Bhavsar
D
D
Department of Biochemistry, Tufts Medical School, Boston, MA 02111.
Zheng
H
H
Drysdale
J
J
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D005293:Ferritins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D004247:DNA; D005293:Ferritins