Cloning and sequencing of the pheU gene for tRNA(Phe) of Thermus thermophilus HB8, and genomic mapping of the pheU and pheST genes.
The gene pheU for tRNA(Phe) from the extreme thermophile Thermus thermophilus HB8 was cloned and sequenced. It differed from the published tRNA(Phe) sequence by one nucleotide. Both the pheU gene and the pheST genes encoding the alpha and beta subunits of phenylalanyl-tRNA synthetase were located on the physical map of the T. thermophilus chromosome, where pheU was present on a hitherto unrecognized HpaI DNA fragment.
Keller
B
B
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, Zürich, Switzerland.
Hennecke
H
H
D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010652:Phenylalanine-tRNA Ligase / Q000235:genetics; D012360:RNA, Transfer, Phe / Q000235:genetics*; D016963:Thermus thermophilus / Q000235:genetics*
D012360:RNA, Transfer, Phe; D010652:Phenylalanine-tRNA Ligase