Morphology of the viable but nonculturable Vibrio cholerae as determined by the freeze fixation technique.
The morphology of the nonculturable Vibrio cholerae strain TSI-4 was examined by the freeze fixation technique of electron microscopy and subsequently four unique structures were found in the fine structure s of this bacterium. The size of the cell was about 2/3 of the growing cell. Although the cell was observed to have an outer membrane as well as the cell membrane and cytoplasm, the outer membrane was undulated and had a surface layer of fine fibers. The peptidoglycan layer was thick and more electron dense than that of normal cells.
Kondo
K
K
Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Takade
A
A
Amako
K
K
D002473:Cell Wall / Q000648:ultrastructure; D005615:Freezing; D008854:Microscopy, Electron; D014734:Vibrio cholerae / Q000254:growth & development / Q000648:ultrastructure*