title: Antitumor effect of neocarzinostatin conjugated to human/mouse chimeric Fab fragments of the monoclonal antibody A7 on human pancreatic carcinoma.
abstract: The anticancer agent neocarzinostatin (NCS) was bound covalently to human/mouse chimeric Fab fragments of the monoclonal antibody A7 to form the conjugate chA7Fab-NCS. The antitumor effect of chA7Fab-NCS was tested by measuring the inhibition of 3H-thymidine incorporation into human pancreatic carcinoma cells. The chA7Fab-NCS was approximately 2.3 times as effective as free NCS against human pancreatic carcinoma cells which reacted with the monoclonal antibody A7. The antitumor activity of chA7Fab-NCS was inhibited by excess chA7Fab. ChA7Fab-NCS had an antitumor effect equivalent to free NCS on human pancreatic carcinoma cells which did not react with the monoclonal antibody A7. ChA7Fab-NCS appears to be a potentially useful conjugate for immunotargeting chemotherapy against pancreatic carcinoma.
authors.affiliation: First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Otsuji
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Yata
authors.forename: H
authors.initials: H
authors.lastname: Nishi
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: K
authors.initials: K
authors.lastname: Taniguchi
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Kotani
authors.forename: N
authors.initials: N
authors.lastname: Yamaoka
authors.forename: K
authors.initials: K
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D004337:Drug Carriers; D006801:Humans; D018796:Immunoconjugates / Q000493:pharmacokinetics*; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism*; D051379:Mice; D010190:Pancreatic Neoplasms / Q000188:drug therapy*; D014407:Tumor Cells, Cultured; D009353:Zinostatin / Q000627:therapeutic use*
chemical_list: D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D018796:Immunoconjugates; D007140:Immunoglobulin Fab Fragments; D009353:Zinostatin