Biosynthetic pathway of 19-noraldosterone in isolated rat glomerulosa cells.
The biosynthetic pathway of 19-noraldosterone (19-noraldo) in isolated rat glomerulosa cells (GC) and fasciculata-reticular cells (FC) was studied by analyzing [14C]pregnenolone metabolism using HPLC and quantification by specific RIA. In GCs, 18,19-dihydroxycorticosterone was detected after 15 min incubation with [14C]pregnenolone, 18-hydroxy-19-norcorticosterone was detected after 30 min and 19-noraldo was detected after 45 min before the appearance of an aldosterone peak. These three mineralocorticoids were not detected in FCs. The results demonstrate that 19-noraldo is synthesized in GCs and then undergoes further metabolism.
Takeda
Y
Y
Second Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.
Miyamori
I
I
Iki
K
K
Yoneda
T
T
Takeda
R
R
D015069:18-Hydroxycorticosterone / Q000031:analogs & derivatives / Q000378:metabolism; D000450:Aldosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D011284:Pregnenolone / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015383:Zona Fasciculata / Q000166:cytology / Q000378:metabolism; D015384:Zona Glomerulosa / Q000166:cytology / Q000378:metabolism*; D015385:Zona Reticularis / Q000166:cytology / Q000378:metabolism
C051358:18-hydroxy-19-norcorticosterone; C051957:18,19-dihydroxycorticosterone; D000450:Aldosterone; D015069:18-Hydroxycorticosterone; D011284:Pregnenolone; C027514:19-noraldosterone