title: Secretion of a temporally controlled cell-associated protein in Arthrobacter aureus: production of the protein and inactivation of its structural gene.
abstract: Arthrobacter aureus secretes 2 proteins of 22 and 100 kDa (P22 and P100, respectively). P22 is a protease, while P100, of unknown function, is associated with the cells before being released into the medium. An immunologically related P100 protein was also found in the culture supernatant of A. ureafasciens. Despite the fact that production of P22 and P100 proteins is induced by growth in the presence of bovine serum albumin, their synthesis is not always induced coordinately. Inactivation of the P100 chromosomal structural gene had no effect on growth characteristics of the mutant under inducing conditions.
authors.affiliation: Laboratoire de Génétique et Physiologie microbiennes, Université de Rennes I, France.
authors.forename: V
authors.initials: V
authors.lastname: Michotey
authors.forename: V
authors.initials: V
authors.lastname: Leret
authors.forename: C
authors.initials: C
authors.lastname: Blanco
mesh_terms: D001173:Arthrobacter / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D066298:In Vitro Techniques; D012697:Serine Endopeptidases / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D012697:Serine Endopeptidases