title: [The use of a biotinated probe for the detection of the genomic RNA of the hepatitis A virus in clinical specimens].
abstract: A detection technique for hepatitis A virus (HAV) RNA by means of molecular hybridization using ss-biotinated DNA probe on the basis of M13 bacteriophage is described. The technique sensitivity reached 1-10 pg of control DNA or 100-500 pg of HAV. The experiments for detection of HAV carriers among the patients and contacts from foci of HAV outbreaks were carried out. A comparative analysis of the above technique and enzyme immunoassay and amplification technique was done and good coincidence of the results was demonstrated.
authors.forename: E V
authors.initials: EV
authors.lastname: Budiak
authors.forename: N M
authors.initials: NM
authors.lastname: Sinagatullina
authors.forename: A V
authors.initials: AV
authors.lastname: Karpukhin
authors.forename: V A
authors.initials: VA
authors.lastname: Naumov
authors.forename: N V
authors.initials: NV
authors.lastname: Mamaeva
authors.forename: V E
authors.initials: VE
authors.lastname: Chizhikov
authors.forename: V F
authors.initials: VF
authors.lastname: Zolotova
authors.forename: N P
authors.initials: NP
authors.lastname: Mertvetsov
mesh_terms: D017104:Bacteriophage M13; D001710:Biotin; D002353:Carrier State / Q000097:blood; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D006506:Hepatitis A / Q000097:blood; D006507:Hepatovirus / Q000235:genetics*; D006801:Humans; D007124:Immunoenzyme Techniques; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral; D001710:Biotin
vernacular_title: Ispol'zovanie biotinirovannogo zonda dlia detektsii genomnoĭ RNK virusa gepatita A v klinicheskikh obraztsakh.