Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes.
Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression.
Holst
L S
LS
Department of Medical and Physiological Chemistry, Lund University, Sweden.
Hoffmann
A M
AM
Mulder
H
H
Sundler
F
F
Holm
C
C
Bergh
A
A
Fredrikson
G
G
D000818:Animals; D003456:Cryptorchidism / Q000201:enzymology / Q000473:pathology; D005260:Female; D017403:In Situ Hybridization; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology; D050356:Lipid Metabolism; D008297:Male; D008698:Mesylates / Q000633:toxicity; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000201:enzymology*; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000201:enzymology / Q000254:growth & development / Q000473:pathology
D008698:Mesylates; D002787:Sterol Esterase; C002994:ethylene dimethanesulfonate