Inhibitory effect of cadmium acetate on synaptosomal ATP diphosphohydrolase (EC 3.6.1.5; apyrase) from adult rat cerebral cortex.
ATP diphosphohydrolase (EC 3.6.1.5; apyrase) is an enzyme that can promote ATP and ADP hydrolysis to AMP plus inorganic phosphate and depends on divalent cations such as Ca2+ or Mg2+. In previous papers we described this enzyme in the synaptosomal fraction from the central and peripheral nervous system. The present report examines whether cadmium acetate could affect the in vitro activity of the enzyme in the synaptosomal fraction from the cerebral cortex of adult male Wistar rats. Cadmium (Cd2+), a heavy metal with neurotoxic effects, inhibited the enzyme in a concentration-dependent manner. All concentrations tested (0.05-1.0 mM) significantly inhibited the hydrolysis of both substrates (ATP and ADP), with the exception of 0.05 mM on ATP hydrolysis. The kinetic data indicate a noncompetitive inhibition between the cations Cd2+ and Ca2+.
Barcellos
C K
CK
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Schetinger
M R
MR
Battastini
A M
AM
Silva
L B
LB
Dias
R D
RD
Sarkis
J J
JJ
D000085:Acetates; D000244:Adenosine Diphosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001081:Apyrase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000201:enzymology*; D006868:Hydrolysis; D007700:Kinetics; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013379:Substrate Specificity; D013574:Synaptosomes / Q000201:enzymology*
D000085:Acetates; D002104:Cadmium; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C028031:cadmium acetate; D000251:Adenosine Triphosphatases; D001081:Apyrase