title: Oxidative stress in hepatocytes and stimulatory state of Kupffer cells after reperfusion differ between warm and cold ischemia in rats.
abstract: Rat liver was kept at 4 degrees C or 37 degrees C in MEM, and reperfused through a closed circulation from the hepatic vein to the portal vein at 37 degrees C with the same solution. Although purine nucleoside phosphorylase and ALT activities were increased in the perfusate, depending on the duration of ischemia at both 4 degrees C and 37 degrees C, the ratio of the latter to the former was significantly higher after 37 degrees C-ischemia than after 4 degrees C-ischemia. The stimulation stage of Kupffer cells evaluated in situ by formazan deposition after liver perfusion with nitro blue tetrazolium and phorbol myristate acetate was elevated after 4 degrees C-ischemia longer than 1 h, but not after 37 degrees C-ischemia. In contrast, the degree of oxidative stress in hepatocytes assessed by formazan deposition after liver perfusion with nitro blue tetrazolium alone was greater after 37 degrees C-ischemia than after 4 degrees C-ischemia. These results suggest that oxidative stress in hepatocytes and the stimulatory state of Kupffer cells after ischemia-reperfusion may differ between 4 degrees C-ischemia and 37 degrees C-ischemia, probably leading to different development of liver damage.
authors.affiliation: Third Department of Internal Medicine, Saitama Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Mochida
authors.forename: M
authors.initials: M
authors.lastname: Arai
authors.forename: A
authors.initials: A
authors.lastname: Ohno
authors.forename: N
authors.initials: N
authors.lastname: Masaki
authors.forename: I
authors.initials: I
authors.lastname: Ogata
authors.forename: K
authors.initials: K
authors.lastname: Fujiwara
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D005562:Formazans / Q000378:metabolism; D007728:Kupffer Cells / Q000378:metabolism*; D008099:Liver / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009580:Nitroblue Tetrazolium / Q000494:pharmacology; D018384:Oxidative Stress; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion / Q000379:methods; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D013696:Temperature; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
chemical_list: D005562:Formazans; D009580:Nitroblue Tetrazolium; D011683:Purine-Nucleoside Phosphorylase; D000410:Alanine Transaminase; D013755:Tetradecanoylphorbol Acetate