[Ontogenetic transformations in the energy metabolism system of the skeletal muscles under different conditions of animal development].
Age-related changes in the general activity of creatin kinase (CrK), mitochondrial fraction of this enzyme (CrKmit), general activity of lactate dehydrogenase (LDH) and its anaerobic fractions (LDHan), content and ratio of cytochromes of the mitochondrial respiration chain, respiration rate of isolated mitochondria on various substrates in the 3rd state have been studied in the skeletal muscle of Wistar rats developing under the conditions of normo- (NK) and hypokinesia (HK). General patterns of changes in these indices have been shown, that do not depend on the conditions of animal development: the minimal CrK and CrKmit activity and increased saturation of the respiration chain with cytochrome b562 during the playing period, days 30 to 40 of postnatal period. The highest level of cytochrome aa3 was observed in the peak of sexual maturation (Day 45). However the conditions of development affect the timing of extreme indices and their level in adult animals. For example, in the animals developing under the NK conditions the highest saturation of the respiration chain with cytochrome b562 was observed on Day 30, while in those developing under the HK conditions by five days later. In 30-day old rats from the NK group the cytochrome aa3 content of skeletal muscle was almost maximal, while in the HK group the maximum was observed only at the peak of sexual maturation. In the adult animals of the HK group the activity of CrKmit, LDH and LDHan was higher, while the content of cytochrome aa3 and saturation of the respiration chain with cytochrome b562 was lower. A lesser b562/aa3 ratio is related to the predominance of NADH2-dependent electron transport pathways of oxidation in the mitochondria from the rat skeletal muscle. Thus, the conditions of development in the early postnatal period, especially during the playing period, determine the state of energy metabolism in the adult skeletal muscle.
Demin
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Androsova
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D000375:Aging / Q000502:physiology*; D000693:Anaerobiosis; D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D006019:Glycolysis / Q000502:physiology; D018476:Hypokinesia / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000254:growth & development*; D051381:Rats; D017208:Rats, Wistar
D003580:Cytochromes; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase
Ontogeneticheskie preobrazovaniia sistemy énergeticheskogo metabolizma skeletnykh myshts pri razlichnykh usloviiakh razvitiia zhivotnykh.