cDNA-derived hypovirus RNA in transformed chestnut blight fungus is spliced and trimmed of vector nucleotides.
Unencapsidated double-stranded viral RNAs belonging to the genus Hypovirus attenuate virulence of the chestnut blight fungus, Cryphonectria parasitica. A full-length cDNA clone of hypovirus CHV1-713 double-stranded RNA was recently shown to be infectious when introduced into the C. parasitica genome by DNA-mediated transformation. In this study, we show that the viral RNA derived from the chromosomally integrated cDNA copy is trimmed of extraneous vector nucleotide sequences. The cDNA-derived viral RNA was also found to contain a 73-bp deletion located within the 5'-noncoding leader sequence as a result of a pre-mRNA splicing event. Implications of these results are discussed in terms of hypovirus RNA replication and anticipated field studies involving engineered hypovirulent C. parasitica strains.
Chen
B
B
Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.
Craven
M G
MG
Choi
G H
GH
Nuss
D L
DL
D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic; D014774:Virulence / Q000235:genetics; D013098:Xylariales / Q000235:genetics* / Q000472:pathogenicity
D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral