title: Hirsutism and acne in women: coordinated radioimmunoassays for eight relevant plasma steroids.
abstract: We developed and validated a coordinated set of RIAs for the following eight steroids in single small aliquots (< or = 1 mL) of plasma: androstenedione, dehydroepiandrosterone, 11-deoxycortisol, 21-deoxycortisol (21-DF), 11 beta-hydroxyandrostenedione, 17 alpha-hydroxypregnenolone (17-Hpreg), 17 alpha-hydroxyprogesterone, and testosterone. Samples were extracted and then chromatographed on celite microcolumns. Radioiodinated tracers were used for two of the assays (17-Hpreg and 21-DF). Tritiated tracers and scintillation proximity assay counting were used to give separation-free procedures for the other six assays, which considerably improved their practicability and reproducibility. The basal and postadrenocorticotropic hormone plasma values for these steroids in normal women sampled in the follicular phase are presented. Finally, the measurement of the eight steroids as a diagnostic method is evaluated with reference to data from 203 patients with hirsutism and (or) acne.
authors.affiliation: Laboratoire de Biologie Hormonale, Hôpital Saint-Louis, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Fiet
authors.forename: J P
authors.initials: JP
authors.lastname: Gosling
authors.forename: H
authors.initials: H
authors.lastname: Soliman
authors.forename: H
authors.initials: H
authors.lastname: Galons
authors.forename: P
authors.initials: P
authors.lastname: Boudou
authors.forename: P
authors.initials: P
authors.lastname: Aubin
authors.forename: A
authors.initials: A
authors.lastname: Belanger
authors.forename: J M
authors.initials: JM
authors.lastname: Villette
authors.forename: R
authors.initials: R
authors.lastname: Julien
authors.forename: J L
authors.initials: JL
authors.lastname: Brérault
mesh_terms: D006907:17-alpha-Hydroxypregnenolone / Q000097:blood; D019326:17-alpha-Hydroxyprogesterone; D000152:Acne Vulgaris / Q000097:blood*; D000293:Adolescent; D000328:Adult; D000735:Androstenedione / Q000031:analogs & derivatives / Q000097:blood; D003350:Cortodoxone / Q000097:blood; D003687:Dehydroepiandrosterone / Q000097:blood; D005260:Female; D005498:Follicular Phase; D006628:Hirsutism / Q000097:blood*; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods* / Q000706:statistics & numerical data; D012016:Reference Values; D012680:Sensitivity and Specificity; D013256:Steroids / Q000097:blood*; D013739:Testosterone / Q000097:blood
chemical_list: D006908:Hydroxyprogesterones; D013256:Steroids; D006907:17-alpha-Hydroxypregnenolone; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; C003582:11-hydroxyandrostenedione; C003556:21-deoxycortisol; D019326:17-alpha-Hydroxyprogesterone; D003350:Cortodoxone