Rat long-term bone marrow culture as a model for transient production of multinucleated giant cells into suspension culture fraction and for steady mineralization process of adherent stromal cells.
Under in vitro conditions rat bone marrow stromal cells induced into osteogenic differentiation by beta-glycerophosphate, exogenous ascorbic acid and dexamethasone are able to produce a mineralized matrix. Here we describe adult rat long-term bone marrow cultures (LTBMC), deprived of these substances. Mineralization process in stromal adherent cells occurred after 1 month of incubation and was proved by means of X-ray electron microprobe. The high content of sulfur in the extracellular matrix surrounding the mineralized nodules suggests non-distrophic pathway of Ca/P deposition. During the first 2 weeks of incubation the extensive production of multinucleated tartrate-resistant acid phosphatase (TRAP) positive and TRAP negative giant cells occurred into the suspension fraction of the cultures. Rat LTBMC may serve as a model for the investigation of differentiation of osteoblastic progenitors and their interactions with multinucleated TRAP positive cells.
Gan
O I
OI
Laboratoire de Recherche sur les Tissus Calcifiés et les Biomatériaux, Faculté de Chirurgie Dentaire, Nantes, France.
Soueidan
A
A
Castagné
A
A
Daculsi
G
G
D000818:Animals; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002458:Cell Fractionation; D002478:Cells, Cultured; D015726:Giant Cells / Q000166:cytology*; D008903:Minerals / Q000378:metabolism*; D008954:Models, Biological; D051381:Rats; D011917:Rats, Inbred Lew; D017154:Stromal Cells / Q000378:metabolism* / Q000502:physiology
D008903:Minerals