Glucose uptake by Listeria monocytogenes Scott A and inhibition by pediocin JD.
Glucose uptake by Listeria monocytogenes Scott A was inhibited by the bacteriocin pediocin JD and by the protonophore carbonyl cyanide m-chlorophenyhydrazone. Experiments with monensin, nigericin, chlorhexidine diacetate, dinitrophenol, and gramicidin, however, showed that glucose uptake could occur in the absence of a proton motive force. L. monocytogenes cell extracts phosphorylated glucose when phosphoenolpyruvate (PEP) was present in the assay mixture, and whole cells incubated with 2-deoxyglucose accumulated 2-deoxyglucose-6-phosphate, indicating the presence of a PEP-dependent phosphotransferase system in this organism. Glucose phosphorylation also occurred when ATP was present, suggesting that a proton motive force-mediated glucose transport system may also be present. We conclude that L. monocytogenes Scott A accumulates glucose by phosphotransferase and proton motive force-mediated systems, both of which are sensitive to pediocin JD.
Christensen
D P
DP
Department of Food Science and Technology, University of Nebraska, Lincoln 68583-0919.
Hutkins
R W
RW
D001430:Bacteriocins / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000378:metabolism*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D010766:Phosphorylation; D011522:Protons; D014475:Uncoupling Agents / Q000494:pharmacology
D001430:Bacteriocins; D011522:Protons; D014475:Uncoupling Agents; D003847:Deoxyglucose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose