Structural and conformational studies of [Ile7] and [Leu7]surfactins from Bacillus subtilis natto.
A novel [Ile7]surfactin (1), which showed anti-human immunodeficiency virus activity, has been isolated from Bacillus subtilis natto. Structural and conformational analysis of the peptide backbone of [Ile7]surfactin was conducted by a combination of various two-dimensional (2D) nuclear magnetic resonance (NMR), circular dichroism (CD) spectroscopy and simulated annealing calculations, compared with a known [Leu7]surfactin (2). Both surfactins were shown to exist in different conformational states in both polar and apolar solvents.
Itokawa
H
H
Tokyo College of Pharmacy, Japan.
Miyashita
T
T
Morita
H
H
Takeya
K
K
Hirano
T
T
Homma
M
M
Oka
K
K
D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000032:analysis* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000737:chemistry*; D015497:HIV-1 / Q000187:drug effects; D055666:Lipopeptides; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000032:analysis* / Q000494:pharmacology; D011487:Protein Conformation
D000998:Antiviral Agents; D055666:Lipopeptides; D010456:Peptides, Cyclic; C087392:surfactin, Ile(7)-; C087391:surfactin, Leu(7)-