[Cloning in the lambda ZAPII/pBluescript system. I. Design of common cDNA libraries].
Here in I summarize some cloning tips that have been revealed during construction of cDNA libraries from rat tissues and mouse stem cell lines in lambda ZAPII, and that improve the cloning efficiency. These improvements can be applied to many different vectors and cDNA cloning systems.
Evtushenko
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D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D005822:Genetic Vectors; D015698:Genomic Library; D051379:Mice; D008970:Molecular Weight; D051381:Rats
D018076:DNA, Complementary
Klonirovanie v sisteme lambda ZAPPII/pBluescript. I. Konstruirovanie obychnykh konotek kDNK.