NGF increases [Ca2+]i in regenerating mature oligodendroglial cells.
The effect of NGF on [Ca2+]i of mature regenerating oligodendroglial cells was investigated by measuring fluo-3 fluorescence. NGF caused transient increases in [Ca2+]i, which could be inhibited by anti-NGF antibody. The rise in [CA2+]i was in part due to influx of extracellular Ca2+ since it was markedly attenuated in Ca2+-free solution. It also depended on release of Ca2+ from intracellular stores as tested by prior depletion with cyclopiazonic acid. These results support a role for Ca2+ in the effects of NGF on oligodendroglial cells.
Engel
J
J
Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany.
Althaus
H H
HH
Kristjansson
G I
GI
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D009836:Oligodendroglia / Q000187:drug effects* / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects; D013552:Swine
D009414:Nerve Growth Factors; D002118:Calcium