Androgen receptor mRNA in the rat ovary and uterus.
The distribution of androgen receptor messenger RNA (ARmRNA) in the reproductive tissues of adult rats was examined by Northern blot analysis and in situ hybridization using ARcRNA probes corresponding to the androgen binding domain of the receptor. About 10-kilobase rat ARmRNA was observed in all tissues examined in the Northern blot analysis. The amount of ARmRNA in the ovary, uterus and testis was less than that in the prostate. In the in situ hybridization study, extensive labeling was observed in the theca cells of the ovary (proestrous) and the endometrium and endometrial glands of the uterus (proestrous). Moderate labeling was observed in the granulosa cells and stromal cells of the ovary and in the myometrium of the uterus. These results were largely in agreement with the distribution of AR previously reported by ligand binding studies. This present in situ hybridization study may provide a useful tool for the analysis of the regional regulation of AR synthesis in the rat female reproductive tissues.
Hirai
M
M
Department of Obstetrics and Gynecology, Yamanashi Medical School, Japan.
Hirata
S
S
Osada
T
T
Hagihara
K
K
Kato
J
J
D000818:Animals; D018076:DNA, Complementary; D004717:Endometrium / Q000737:chemistry; D004971:Estrus; D005260:Female; D017403:In Situ Hybridization; D008297:Male; D009215:Myometrium / Q000737:chemistry; D010053:Ovary / Q000737:chemistry* / Q000166:cytology; D011467:Prostate / Q000737:chemistry; D015347:RNA Probes; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000235:genetics*; D012680:Sensitivity and Specificity; D013737:Testis / Q000737:chemistry; D014599:Uterus / Q000737:chemistry*
D018076:DNA, Complementary; D015347:RNA Probes; D016372:RNA, Antisense; D012333:RNA, Messenger; D011944:Receptors, Androgen