Failure of adenine nucleotide and water content of cold-stored rat livers to serve as graft viability indices for transplantation.
A study was undertaken to determine whether the cellular viability of the liver graft and posttransplantation outcome could the assessed by the graft energy status or change in water content prior to the transplantation procedure. These measurements were performed in a rat liver transplantation model. In a first set of experiments, the time for 50% survival of grafts preserved with UW solution or saline solution was determined. Grafts preserved with UW solution could be cold-stored for 19.9 h and grafted with a 50% success rate. The corresponding figure for saline-preserved grafts was 6.2 h. The energy status and water content of liver grafts preserved with UW solution for 19.9 h and livers preserved with saline solution for 6.2 h were compared. There were significant differences between livers preserved with UW solution and saline solution for ATP (p < 0.01), total adenine nucleotides (p < 0.05), ATP/ADP (p < 0.05) and energy charge levels (p < 0.05). ATP concentrations in the donor livers did not decrease significantly during cold storage when the success rate decreased from 100 to 0% after liver transplantation. The livers preserved with NaCl solution took up water during preservation. In contrast, the water content was reduced slightly in the livers preserved with UW solution and the differences were significant at all times. During cold storage using either the UW solution or saline solution for preservation, there were no significant time-related changes in the water content of the liver graft when the posttransplantation success rate decreased from 100 to 0% after liver transplantation.(ABSTRACT TRUNCATED AT 250 WORDS)
Svensson
G
G
Department of Thoracic and Cardiovascular Surgery, Sahlgrenska Hospital, Göteborg, Sweden.
Soussi
B
B
Wiklund
L
L
Karlberg
I
I
D000227:Adenine Nucleotides / Q000378:metabolism*; D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001834:Body Water / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015925:Cryopreservation; D005260:Female; D005978:Glutathione; D006085:Graft Survival; D007328:Insulin; D008099:Liver / Q000378:metabolism*; D016031:Liver Transplantation; D019188:Organ Preservation Solutions; D009994:Osmolar Concentration; D011887:Raffinose; D051381:Rats; D011920:Rats, Inbred WF
D000227:Adenine Nucleotides; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose