title: Fusion properties of cells constitutively expressing human parainfluenza virus type 4A haemagglutinin-neuraminidase and fusion glycoproteins.
abstract: We established HeLa cell lines that constitutively expressed the fusion (F) and/or haemagglutinin-neuraminidase (HN) glycoproteins of human parainfluenza virus type 4A (PIV-4A) and used them to analyse the roles of these glycoproteins in virus-induced cell fusion. No syncytium formation occurred, even in HeLa cells expressing both the F and HN proteins (HeLa-4aF+HN cells). Also no syncytium was found in a mixed culture of cells expressing the F protein (HeLa-4aF) and the HN protein (HeLa-4aHN). Syncytia were observed in HeLa-4aF cells transfected with the HN gene, but no syncytium formation was found in HeLa-4aHN cells transfected with the F gene. Co-cultivation of HeLa-4aF+HN cells with HeLa-4aF cells generated large polykaryocytes, whereas co-cultivation with HeLa-4aHN cells induced no cell fusion. Infection of HeLa-4aF cells with PIV-4A generated large syncytia and degenerated nuclei, whereas little or no polykaryocytes were found in HeLa-4aHN cells infected with PIV-4A. From the above findings, the following conclusions were drawn: (i) the expression of both the F and HN proteins in the same cell is necessary for cell fusion; (ii) the expression of the F protein alone enhances susceptibility to cell fusion; (iii) the constitutive expression of the HN protein promotes resistance to paramyxovirus-induced cell fusion.
authors.affiliation: Department of Microbiology, Mie University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nishio
authors.forename: M
authors.initials: M
authors.lastname: Tsurudome
authors.forename: H
authors.initials: H
authors.lastname: Komada
authors.forename: M
authors.initials: M
authors.lastname: Kawano
authors.forename: N
authors.initials: N
authors.lastname: Tabata
authors.forename: H
authors.initials: H
authors.lastname: Matsumura
authors.forename: N
authors.initials: N
authors.lastname: Ikemura
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D002459:Cell Fusion; D003588:Cytopathogenic Effect, Viral; D015870:Gene Expression; D005814:Genes, Viral; D015726:Giant Cells / Q000502:physiology; D015707:HN Protein / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells / Q000821:virology; D006801:Humans; D019158:N-Acetylneuraminic Acid; D018108:Respirovirus / Q000502:physiology*; D012794:Sialic Acids / Q000032:analysis; D014162:Transfection; D014760:Viral Fusion Proteins / Q000235:genetics / Q000502:physiology*; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D015707:HN Protein; D012794:Sialic Acids; D014760:Viral Fusion Proteins; D015678:Viral Structural Proteins; D019158:N-Acetylneuraminic Acid