Antibody against interleukin-6 reduces inflammation and numbers of cysts in brains of mice with toxoplasmic encephalitis.
Treatment of toxoplasmic encephalitis in C57BL/6 mice with monoclonal antibody (MAb) against interleukin-6 (IL-6) resulted in a remarkable decrease in the number of foci of acute inflammation in their brains caused by proliferation of tachyzoites. In brains of mice treated with isotype control MAb and those treated with anti-IL-6 MAb, tachyzoites were observed only in foci of acute inflammation. Immunoperoxidase staining revealed a greatly diminished frequency of tachyzoites in brains of mice treated with anti-IL-6 MAb. Of interest, treatment with MAb against IL-6 was also associated with reduced numbers of Toxoplasma gondii cysts in the brains and with higher serum levels of gamma interferon than in control mice. Paradoxically, the mice treated with anti-IL-6 MAb had higher serum levels of IL-6 as measured by an enzyme-linked immunosorbent assay than controls. These results revealed the importance of IL-6 in the immunopathogenesis of murine toxoplasmic encephalitis.
Suzuki
Y
Y
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301.
Yang
Q
Q
Conley
F K
FK
Abrams
J S
JS
Remington
J S
JS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D000913:Antibodies, Protozoan / Q000627:therapeutic use*; D001921:Brain / Q000473:pathology; D003560:Cysts / Q000628:therapy; D004660:Encephalitis / Q000628:therapy*; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007249:Inflammation / Q000628:therapy; D007371:Interferon-gamma / Q000097:blood; D015850:Interleukin-6 / Q000097:blood / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014124:Toxoplasmosis, Animal / Q000628:therapy*; D016781:Toxoplasmosis, Cerebral / Q000628:therapy*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D015850:Interleukin-6; D007371:Interferon-gamma