title: Susceptibility to adenosine agonists of giant migrating contraction induced by glycerol enema in anesthetized rats.
abstract: The present study examined whether adenosine agonists influence the occurrence of giant migrating contractions (GMCs) induced by glycerol enema (65%, 2 ml/kg) in rats. Catheter pressure transducers were used to measure the colonic luminal manometric alterations. The adenosine A1 agonists (2S)-N6-(2-endo-norbornyl)adenosine ((S)-ENBA) (10 micrograms/kg, i.v.) and N6-cyclohexyladenosine (30 micrograms/kg, i.v.) abolished the GMCs, whereas the adenosine A2 agonist 2-[p-(2-carboxyethyl)phenethylamino]-5'-N-ethylcarboxamidoadenosin e (CGS 21680) (30-300 micrograms/kg, i.v.) failed to influence the GMCs. The suppressive action of (S)-ENBA on the GMCs was entirely counteracted by the peripheral adenosine antagonist 8-(p-sulfophenyl)theophylline (10 mg/kg, i.v.). The present observations suggest that the adenosine A1 agonist suppresses the GMCs via peripheral adenosine receptors.
authors.affiliation: Pharmaceutical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Shizuoka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tomaru
authors.forename: A
authors.initials: A
authors.lastname: Ishii
authors.forename: N
authors.initials: N
authors.lastname: Kishibayashi
authors.forename: A
authors.initials: A
authors.lastname: Karasawa
mesh_terms: D000241:Adenosine / Q000819:agonists* / Q000031:analogs & derivatives / Q000494:pharmacology; D000758:Anesthesia; D000818:Animals; D003106:Colon / Q000187:drug effects* / Q000502:physiology; D004733:Enema; D005769:Gastrointestinal Motility / Q000187:drug effects*; D005990:Glycerol / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D016065:Myoelectric Complex, Migrating / Q000187:drug effects; D009636:Norbornanes / Q000494:pharmacology; D010627:Phenethylamines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: C089285:N(6)-(2-endo-norbornyl)adenosine; D009636:Norbornanes; D010627:Phenethylamines; C061282:2-(4-(2-carboxyethyl)phenethylamino)-5'-N-ethylcarboxamidoadenosine; C027513:N(6)-cyclohexyladenosine; D000241:Adenosine; D005990:Glycerol