[A method for visualization of chromosomes from human sperm and its application].
A cytogenetic technique for analysis of human sperm chromosomes is described in this article. It is based on penetration of hamster oocytes after enzymatic treatment and inhibition of cell division during pronuclei stage. Two different methods of capacitation of sperm, that is necessary for them to penetrate the eggs have been considered. Attention is paid to manipulations and technical difficulties on consecutive steps of realisation of this method. This method is applied mainly in analysis of chromosome segregation in males, that are carriers of chromosome aberrations. The possibility of using this method in other clinical cases and basic research has been presented.
Barczyk
A
A
Zakładu Biologii Instytutu Nauk Podstawowych Wojskowej Akademii Medycznej w Lodzi.
D000818:Animals; D002869:Chromosome Aberrations / Q000235:genetics*; D003582:Cytogenetics / Q000379:methods*; D005306:Fertilization; D006168:Guinea Pigs; D006801:Humans; D007621:Karyotyping; D008297:Male; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000166:cytology*
Metoda uwidocznienia chromosomów z plemników ludzkich i jej zastosowanie.