title: Lipophorin as a lipid carrier in the hemolymph of the horseshoe crab, Limulus polyphemus.
abstract: A high-density lipoprotein (HDL) was obtained from the hemolymph of Limulus polyphemus in yields generally less than 30 micrograms/ml by ultracentrifugal flotation. SDS-PAGE revealed two apolipoproteins with masses similar to those of apolipophorins (apoLp-I, 265 +/- 14 kDa; apoLp-II, 89 +/- 6 kDa). Lipid composition was different from both insect lipophorin and crustacean HDL, and showed less diacylglycerols than triacylglycerols (3.8% and 36.2% of total lipids, respectively). Since Limulus polyphemus is closely related to precambrian chelicerates, our results confirm that lipophorin was present early in the evolution of arthropods.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brasil.
authors.forename: K G
authors.initials: KG
authors.lastname: Rehn
authors.forename: R F
authors.initials: RF
authors.lastname: Lee
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000097:blood / Q000302:isolation & purification; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D006458:Hemolymph / Q000737:chemistry*; D006737:Horseshoe Crabs; D008074:Lipoproteins; D008075:Lipoproteins, HDL / Q000097:blood / Q000302:isolation & purification; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D001053:Apolipoproteins; D002352:Carrier Proteins; D008074:Lipoproteins; D008075:Lipoproteins, HDL; C032830:lipophorin