Delivery of plasmid DNA to glial cells using pH-sensitive immunoliposomes.
Immunoliposomes were constructed with an antibody specific to glial cells. They were used to examine the specificity and efficacy of cell type plasmid transfection. Liposomes contained a beta-galactosidase gene under control of an SV-40 promotor. Two different monoclonal antibodies of a different subclass, IgM and IgG, were examined for their targeting ability using immunoliposomes. Cultured C6 glioma (specific target cell type) and NIH 3T3 (control cell type, fibroblast) cells were transfected using these immunoliposomes. Results indicate a three-fold increase in transfection by the glial specific immunoliposomes, "gliasomes", in glial cell culture over control liposomes. Gliasomes were exposed to NIH 3T3 cells and showed no enhanced transfection over control liposomes. Gliasomes were tested for their specificity by the addition of excess antibody to the cell culture in order to saturate specific receptors on C6 glioma cells. Results indicate a reduced transfection, nearly three-fold, in cells that were saturated with excess antibody prior to exposure to the immunoliposomes.
Holmberg
E G
EG
Department of Chemistry/Physics, University of Alaska Anchorage 99508.
Reuer
Q R
QR
Geisert
E E
EE
Owens
J L
JL
D016475:3T3 Cells; D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000008:administration & dosage* / Q000378:metabolism; D004337:Drug Carriers; D004926:Escherichia coli / Q000201:enzymology; D005910:Glioma / Q000378:metabolism*; D007672:Kidney Cortex / Q000166:cytology; D008081:Liposomes; D051379:Mice; D009457:Neuroglia / Q000378:metabolism*; D010957:Plasmids / Q000008:administration & dosage*; D051381:Rats; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D000911:Antibodies, Monoclonal; D004269:DNA, Bacterial; D004337:Drug Carriers; D008081:Liposomes; D001616:beta-Galactosidase