title: Distribution of cholesterol among its carriers in the bile of male and female hamsters.
abstract: The distribution of cholesterol among its carriers was studied in the bile of male and female hamsters. Sasco hamsters (Sasco Inc., Omaha, NE) were fed a semipurified diet with 0.0% cholesterol and 4% butterfat (group 1, males; group 4, females); a semipurified diet with 0.3% cholesterol and 1.2% palmitic acid (group 2, males; group 5, females); and a semipurified diet with 0.3% cholesterol and 4% safflower oil (group 3, males; group 6, females). At the end of six weeks, gallstones were found only in male hamsters receiving both cholesterol and dietary fat (fatty acid) (incidence of cholesterol stones: 90% in group 2; 22% in group 3). The biliary cholesterol carriers were separated and isolated from the bile of the hamsters by gel filtration chromatography, using the method of Pattinson [Pattinson, N.R., Willis, K.E., and Frampton, C.M. (1991) J. Lipid Res. 32, 205-214]. In those male hamsters that formed cholesterol gallstones, significant amounts of cholesterol were present in the void volume which contained large cholesterol phospholipid vesicles (void volume vesicles) (23% in group 2 and 15% in group 3). Smaller cholesterol/phospholipid vesicles were eluted next (fractions 30-45) and contained 15% of biliary cholesterol in group 2 and 21% in group 3. The remainder of the cholesterol was associated with mixed cholesterol/phospholipid/bile salt micelles. The cholesterol/phospholipid ratio was larger in both the void volume vesicles and small vesicles (2.40 and 1.48 in group 2; 2.56 and 1.33 in group 3, respectively) compared to the micelles (about 0.3 in groups 2 and 3).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, Beth Israel Medical Center, New York, New York 10003.
authors.forename: T
authors.initials: T
authors.lastname: Mikami
authors.forename: B I
authors.initials: BI
authors.lastname: Cohen
authors.forename: Y
authors.initials: Y
authors.lastname: Mikami
authors.forename: N
authors.initials: N
authors.lastname: Ayyad
authors.forename: E H
authors.initials: EH
authors.lastname: Mosbach
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D002769:Cholelithiasis / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D006224:Cricetinae; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D008647:Mesocricetus; D008823:Micelles; D010743:Phospholipids / Q000378:metabolism*; D012727:Sex Characteristics
chemical_list: D008823:Micelles; D010743:Phospholipids; D002784:Cholesterol