title: Determination of plasma cocaine and ethylcocaine (cocaethylene) in mice using gas chromatography-mass spectrometry and deuterated internal standards.
abstract: A gas chromatographic-mass spectrometric method is described for the determination of cocaine and ethylcocaine (cocaethylene) from mouse plasma microsamples (50 microliters). [2H3]Cocaine and [2H5]ethylcocaine served as internal standards, analytical separations were performed on a (5% phenyl)methylpolysiloxane capillary column, and detection was by selected-ion monitoring of electron-impact generated fragment ions [M - CO2Ph]. Pilot study plasma concentrations of ethylcocaine following coadministration of cocaine and ethanol were less than 5% of the parent drug.
authors.affiliation: Department of Pharmaceutical Sciences, Medical University of South Carolina, Charleston 29425-2303.
authors.forename: S R
authors.initials: SR
authors.lastname: Miller
authors.forename: A L
authors.initials: AL
authors.lastname: Salo
authors.forename: W O
authors.initials: WO
authors.lastname: Boggan
authors.forename: K S
authors.initials: KS
authors.lastname: Patrick
mesh_terms: D000818:Animals; D003042:Cocaine / Q000031:analogs & derivatives* / Q000097:blood* / Q000138:chemical synthesis; D003903:Deuterium / Q000032:analysis; D018765:Dopamine Uptake Inhibitors / Q000097:blood* / Q000737:chemistry; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling; D051379:Mice; D008810:Mice, Inbred C57BL; D012015:Reference Standards
chemical_list: D018765:Dopamine Uptake Inhibitors; D003903:Deuterium; C066444:cocaethylene; D003042:Cocaine