Regular initiation of translation of Plasmodium berghei aldolase-2 after pre-mRNA splicing.
In Plasmodium falciparum aldolase a UAG or a regular AUG codon has been proposed for the initiation of ribosomal protein synthesis. A UAG codon present at the beginning of the coding sequence of the aldolase 2 gene (aldo-2) of Plasmodium berghei is not recognised in vitro as an initiation codon, which suggests addition of a regular AUG codon by mRNA splicing. Sequence analysis of cDNA amplified by the reversed polymerase chain reaction reveals addition of an ATG codon with a splice donor consensus sequence to the aldo-2 exon. By the same technique and northern blot analysis, substantial amounts of partially spliced P. berghei aldo-2 precursor mRNA are detected which could explain the isolation of immature P. falciparum aldolase cDNA clones starting with a stop codon.
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Department PRTB, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003062:Codon; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D017125:Genes, Protozoan / Q000235:genetics; D016678:Genome; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D010442:Peptide Chain Initiation, Translational; D010962:Plasmodium berghei / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing; D017422:Sequence Analysis, DNA
D003062:Codon; D018076:DNA, Complementary; D012322:RNA Precursors; D005634:Fructose-Bisphosphate Aldolase