Differential induction of Fos protein and a Fos-related antigen following acute and repeated cocaine administration.
The present study examined the effects of a single and five once daily injections of cocaine on the expression of c-fos mRNA, Fos protein and Fos-related antigens (Fra) in the striatum. A single injection (40 mg/kg, i.p.), which induces locomotion, increased the expression of c-fos mRNA, Fos protein and a 35 kDa Fra. In contrast, five injections (40 mg/kg, i.p.) given once a day, which induces even more behavioral stimulation, diminished the increase in c-fos mRNA and Fos protein expression. However, the cocaine-induced Fra expression was sustained and not reduced after the five injections. The results demonstrate that cocaine-induced expression of the Fos/Fra gene family is dynamic and the profile of gene transcription and translation in the striatum changes when animals are behaviorally more sensitive to cocaine.
Rosen
J B
JB
Biological Psychiatry Branch, National Institute of Mental Health, National Institute of Dental Research, Bethesda, MD 20892.
Chuang
E
E
Iadarola
M J
MJ
D000818:Animals; D003042:Cocaine / Q000008:administration & dosage*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004334:Drug Administration Schedule; D009043:Motor Activity / Q000187:drug effects; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D013239:Stereotyped Behavior / Q000187:drug effects
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D003042:Cocaine