Virus-like particles in MDCK cells persistently infected with Borna disease virus.
A line of Madin Darby canine kidney (MDCK) cells persistently infected with Borna disease virus was examined by electron microscopy. Thin sections revealed the presence of intracytoplasmic virus-like particles ranging from 50-100 nm in diameter. Nuclei of the infected cells exhibited accumulation of electron-dense granular structures 15-18 nm in diameter. The intracytoplasmic particles were roughly spherical with a limiting membrane, suggesting the presence of a lipid-containing envelope. The internal structure consisted of strand-like material which in some cases was condensed underneath the envelope. The possible relationship of these particles to Borna disease virions is discussed.
Compans
R W
RW
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322.
Melsen
L R
LR
de la Torre
J C
JC
D000818:Animals; D001891:Borna disease virus / Q000254:growth & development / Q000648:ultrastructure*; D002460:Cell Line; D002467:Cell Nucleus / Q000821:virology*; D003593:Cytoplasm / Q000821:virology*; D004285:Dogs; D004847:Epithelial Cells; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure*; D007668:Kidney; D008854:Microscopy, Electron; D014771:Virion / Q000648:ultrastructure