Stabilized hemoglobin vesicles.
The Hb-vesicles which encapsulate the purified and concentrated Hb more than 40 g/dl with a uni- or bi-lamellar membrane are prepared by extruding the dispersion of mixed lipids through membrane filters (final pore size: 0.2 micron phi). They transport large amount of oxygen with satisfying rheological properties such as oncotic pressure and solution viscosity. Oxygen affinity of the Hb-vesicles is adjusted so as to exceed the ability of oxygen transport of human blood by coencapsulating allosteric effectors in the Hb-vesicles. The solution is sterilizable because of the diameter of Hb vesicles less than 0.2 micron phi. The Hb-vesicles are stabilized by using polyphospholipid or glycolipid as membrane components. No change in oxygen affinity and particle size was confirmed during long time storage at 4 degrees C. The stabilized Hb-vesicles can also be stored as frozen or dried state. The dried Hb-vesicles are regenerated by simply adding pure water. Simple in vitro test indicates that Hb-vesicles have the reduced inhibitory action of Hb to the EDRF-mediated vasorelaxation.
Tsuchida
E
E
Department of Polymer Chemistry, Waseda University, Tokyo, Japan.
D000109:Acetylcholine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004355:Drug Stability; D004356:Drug Storage; D005260:Female; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D008297:Male; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010316:Particle Size; D014677:Pharmaceutical Vehicles; D011817:Rabbits; D012996:Solutions; D014664:Vasodilation / Q000187:drug effects
D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes; D014677:Pharmaceutical Vehicles; D012996:Solutions; C016484:hemoglobin, stroma free; D009569:Nitric Oxide; D000109:Acetylcholine