[A method of preparing specimens for the electron microscopic study of bacterial fimbrial structures].
A method for making bacterial preparations for studies of fimbrial structures by electron microscopy has been developed, making use of impressions of 'young' colonies in agar microscopy. The suggested method involves three steps: cultivation of the examined bacteria in lamellar media till they attain the 'young' age; agar microscopy of the grown culture and making of impressions from the selected sites; contrasting of the objects and drying of the preparations. The new method is simple, economic, highly reliable and yields good results. It maximally rules out the factors conducive to rupture and injury of fimbria, observed in traditional method of making the preparations.
Pokhil
S I
SI
Smelianskaia
M V
MV
D001431:Bacteriological Techniques; D003470:Culture Media; D010861:Fimbriae, Bacterial / Q000648:ultrastructure*; D007711:Klebsiella pneumoniae / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods
D003470:Culture Media
Sposob prigotovleniia preparatov dlia élektronno-mikroskopicheskogo issledovaniia fimbrial'nykh struktur bakteriĭ.