Influence of age on the production of Fos and Jun by influenza virus-exposed T cells.
This study investigated age-related T cell responses after in vitro exposure to influenza A virus. Mononuclear leukocytes from young or elderly persons were sham-exposed or exposed to influenza virus for 1, 24, and 72 h. Immunofluorescent staining and flow cytometric analysis were then used to detect T cells producing the transcriptional regulating proteins Fos and Jun. Fewer virus-exposed cells from elderly donors stained for Fos and Jun at each data point compared with cells from young donors. Flow cytometric analysis also showed that at 72 h of virus exposure fewer T cells from the elderly produced interferon-gamma (IFN-gamma), suggesting a link between the magnitude of the Fos and Jun and IFN-gamma responses. Thus, failure of virus-exposed T cells to produce Fos and Jun could contribute to the increase in illness due to influenza virus in the elderly.
Salkind
A R
AR
Department of Medicine, University of Mississippi School of Medicine, Jackson 39216.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood / Q000276:immunology / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007113:Immunity, Innate; D009980:Influenza A virus / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D008875:Middle Aged; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000821:virology*
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D007371:Interferon-gamma