Determination of acenocoumarol in human plasma by capillary gas chromatography with mass-selective detection.
A method for the determination of acenocoumarol in human plasma by capillary gas chromatography-mass-selective detection is described. After addition of a structurally related analogue as the internal standard, the compounds are extracted from plasma at acidic pH into toluene, back-extracted with a basic solution and re-extracted from hydrochloric acid solution with toluene, which is then evaporated to dryness. The compounds are converted into their methyl derivatives, which are determined by gas chromatography using a mass-selective detector at m/z 324 for acenocoumarol and m/z 338 for the internal standard. The reproducibility and accuracy of the method were found to be suitable over the acenocoumarol concentrations range 2.2-74 nmol/l. The method could be considered as selective for acenocoumarol in the presence of its major metabolites in plasma.
Pommier
F
F
Laboratories Ciba-Geigy, Biopharmaceutical Research Center, Rueil Malmaison, France.
Ackermann
R
R
Sioufi
A
A
Godbillon
J
J
D000074:Acenocoumarol / Q000097:blood*; D002138:Calibration; D002197:Capillary Action; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011786:Quality Control; D012680:Sensitivity and Specificity; D014050:Toluene
D014050:Toluene; D000074:Acenocoumarol