title: Detection of genetic variations in serotype I isolates of infectious bursal disease virus using polymerase chain reaction and restriction endonuclease analysis.
abstract: Reverse transcription with polymerase chain reaction (PCR) followed by restriction endonuclease analysis detected genetic variations among serotype I isolates of infectious bursal disease virus (IBDV). Using a set of synthetic primers derived from the large genome segment of APHIS-IBDV, the hypervariable region (AccI-SpeI fragment) located in the VP2 gene was amplified. With all strains, a cDNA fragment of approximately 643 bp was amplified, indicating that there were no apparent deletions or insertions in this region among isolates. Fragments amplified from 9 isolates were digested with 14 restriction enzymes. Restriction fragment profiles generated by restriction enzymes NaeI, StuI, TaqI, and SacI, showed genetic variations among isolates. This study provided a simple and sensitive method for detection of genetic variations among isolates that are closely related serologically and could not be differentiated using current serologic methods.
authors.affiliation: Department of Poultry Science, Auburn University, AL 36849-5416.
authors.forename: H J
authors.initials: HJ
authors.lastname: Liu
authors.forename: J J
authors.initials: JJ
authors.lastname: Giambrone
authors.forename: T
authors.initials: T
authors.lastname: Dormitorio
mesh_terms: D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000662:veterinary / Q000821:virology; D002645:Chickens; D017931:DNA Primers / Q000235:genetics; D004262:DNA Restriction Enzymes; D018076:DNA, Complementary / Q000235:genetics; D014644:Genetic Variation; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000821:virology; D012703:Serotyping; D014773:Virology / Q000379:methods
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004262:DNA Restriction Enzymes