[Synthesis of new fragments of VP1 protein fragments from foot and mouth disease virus type A22. Synthesis of fragments 134-139, 134-145, 140-145, 150-155, and 150-159].
Fragments 134-145 and 150-159 of the antigenic-region of the VP1 protein of the A22 foot-and-mouth disease virus were synthesized by classic methods of peptide chemistry with isobutyl chloroformate as a coupling reagent. After purification by HPLG and amino acid analysis, the free peptides H-Gly-Lys-Tyr-Ser-Ala-Gly-Gly-Leu-Gly-Arg-Arg-Gly-OH and H-Leu-Ala-Ala-Arg-Val-Ala-Lys-Gln-Leu-Pro-OH were conjugated with BSA by means of N,N-dicyclohexylcarbodiimide. The conjugates were used, with complete Freund adjuvant, for immunization on guinea pigs. The antibodies formed had a virus neutralization activity.
Khalikov
Sh Kh
ShKh
Alieva
S V
SV
Ashurov
S G
SG
D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004024:Dicyclohexylcarbodiimide; D005561:Formates; D006168:Guinea Pigs; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D012710:Serum Albumin, Bovine; D014764:Viral Proteins / Q000737:chemistry*
D005561:Formates; D007202:Indicators and Reagents; D010446:Peptide Fragments; D014764:Viral Proteins; D012710:Serum Albumin, Bovine; D004024:Dicyclohexylcarbodiimide; C039311:isobutyl chloroformate
Sintez novykh fragmentov belka VP1 virusa iashchura tipa A22. Sintez fragmentov 134-139, 134-145, 140-145, 150-155, and 150-159.