title: High-performance liquid chromatography with UV detection and diode-array UV confirmation of isonicotinic acid hydrazide in cattle milk.
abstract: A method for the determination of isonicotinic acid hydrazide (isoniazid) in milk was developed. Milk was deproteinized with trichloroacetic acid. Isoniazid was condensed with cinnamaldehyde and assayed on a reversed-phase HPLC system, with good sensitivity and accuracy (10 micrograms/l) with UV detection at 330 nm. Use of solid-phase extraction with a C18 cartridge allows the detection limit to be lowered to 0.1 microgram/l with UV detection and confirmation of isoniazid hydrazone from the diode-array UV spectrum.
authors.affiliation: Laboratorio di Sanità Pubblica USSL No. 40, Ivrea (TO), Italy.
authors.forename: A
authors.initials: A
authors.lastname: Defilippi
authors.forename: G
authors.initials: G
authors.lastname: Piancone
authors.forename: R
authors.initials: R
authors.lastname: Costa Laia
authors.forename: S
authors.initials: S
authors.lastname: Balla
authors.forename: G P
authors.initials: GP
authors.lastname: Tibaldi
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D007538:Isoniazid / Q000032:analysis* / Q000097:blood; D008892:Milk / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007202:Indicators and Reagents; D007538:Isoniazid