Fimbria-mediated adherence of Candida albicans to glycosphingolipid receptors on human buccal epithelial cells.
Candida albicans is an opportunist fungal pathogen that has the ability to adhere to host cell surface receptors via a number of adhesins. Yu et al. (L. Yu, K. K. Lee, K. Ens, P. C. Doig, M. R. Carpenter, W. Staddon, R. S. Hodges, W. Paranchych, and R. T. Irvin, Infect. Immun. 62:2834-2842, 1994) described the purification and initial characterization of a fimbrial adhesin from C. albicans. In this paper, we show that C. albicans fimbriae also bind to asialo-GM1 [gangliotetraosylceramide: beta Gal(1-3)beta GalNAc(1-4) beta Gal(1-4)beta Glc(1-1)Cer] immobilized on microtiter plates in a saturable and concentration-dependent manner. C. albicans fimbrial binding to exfoliated human buccal epithelial cells (BECs) was inhibited by asialo-GM1 in in vitro binding assays. The fimbriae interact with the glycosphingolipid receptors via the carbohydrate portion of the receptors, since fimbriae were observed to bind to synthetic beta GalNAc(1-4)beta Gal-protein conjugates and the disaccharide was able to inhibit binding of fimbriae to BECs in in vitro binding assays. We conclude from these results that the C. albicans yeast form expresses a fimbrial adhesin that binds to glycosphingolipids displayed on the surface of human BECs.
Yu
L
L
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Lee
K K
KK
Sheth
H B
HB
Lane-Bell
P
P
Srivastava
G
G
Hindsgaul
O
O
Paranchych
W
W
Hodges
R S
RS
Irvin
R T
RT
D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006028:Glycosphingolipids / Q000378:metabolism; D006801:Humans; D008297:Male; D009055:Mouth / Q000166:cytology / Q000378:metabolism*; D015388:Organelles / Q000502:physiology; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism
D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; D006023:Glycoproteins; D006028:Glycosphingolipids; D011956:Receptors, Cell Surface