In vitro effects of aspirin on malondialdehyde formation and on activity of antioxidant and some metal-containing enzymes.
The in vitro effects of aspirin in different concentrations on malondialdehyde formation and on the activity of antioxidant and some metal-containing enzymes in rat liver homogenate and erythrocytes were studied. Aspirin showed a biphasic dependence on concentration: low concentrations (to 1.0 mM) stimulated the spontaneously formed malondialdehyde in the liver homogenate and the high concentration (5.0 mM) inhibited it; all aspirin concentrations tested had no effect on the liposomes; 5.0 mM aspirin inhibited the Fe(2+)-induced lipid peroxidation in the liver homogenate but had an opposite effect on the liposomes. Aspirin dose-dependently inhibited nitro-blue tetrazolium reduction and decreased deoxyribose degradation. The higher aspirin concentrations inhibited the activity of antioxidant and some metal-containing enzymes. It is suggested that these aspirin effects are determined by the aspirin-metal complexes formed rather than by aspirin itself.
Kirkova
M
M
Institute of Physiology, Bulgaria Academy of Sciences, Sofia.
Ivancheva
E
E
Russanov
E
E
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology*; D003855:Deoxyribose / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004798:Enzymes / Q000097:blood; D005296:Ferrous Compounds / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000975:Antioxidants; D004798:Enzymes; D005296:Ferrous Compounds; D016166:Free Radical Scavengers; D008081:Liposomes; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D009580:Nitroblue Tetrazolium; D008315:Malondialdehyde; D003855:Deoxyribose; D004492:Edetic Acid; D001241:Aspirin