Cross-linking of iodinated prolactin to rat liver nuclear protein.
The specific binding of prolactin (PRL) in rat liver nuclei have been examined. Following cross-linking with the use of 125I-rat prolactin and autoradiography the evidence is presented for the specific binding of PRL to a nuclear protein with low Mr of about 7000. This study shows that the PRL binder in rat liver nuclei is a small protein and probably differs from molecular form of the rat liver membrane prolactin receptor.
Knopp
J
J
Intitute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava.
Zaliberová
Y
Y
Jurcovicová
J
J
Torda
T
T
Brtko
J
J
D000818:Animals; D001345:Autoradiography; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes; D008099:Liver / Q000166:cytology / Q000648:ultrastructure*; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley
D007457:Iodine Radioisotopes; D009687:Nuclear Proteins; D011388:Prolactin