title: Transforming growth factor beta-1 facilitates establishing clonal populations of ovine muscle satellite cells.
abstract: Myogenic cells isolated from lamb fetuses (approximately mid-gestation) exhibited a concentration-dependent decrease in myogenic cell proliferation in response to transforming growth factor (TGF) beta-1 (P < .001). Half-maximal inhibition of proliferation occurred at approximately .05 ng of TGF beta-1/mL and maximal inhibition of proliferation occurred at approximately .1 ng of TGF beta-1/mL. The specificity of this inhibition was confirmed by neutralization of the activity following exposure to a TGF beta antibody. The TGF beta-1 also suppressed proliferation of ovine satellite cells isolated from 5-d-old lambs (P < .0035), but to a lesser extent than observed for embryonic cells. In contrast, TGF beta-1 did not significantly suppress serum-stimulated proliferation of ovine satellite cells isolated from 30- or 150-d-old lambs. Similarly, TGF beta-1 did not suppress proliferation of skeletal muscle fibroblast-like cells isolated from either fetal lambs or 150-d-old lambs. In fact, proliferation of fibroblast-like cells derived from embryonic ovine muscle was enhanced by exposure to TGF beta-1 at all levels tested; however, a concentration-dependent response was not observed. Media transfer experiments showed that conditioning of culture media by postnatally derived cells did not render TGF beta-1 inactive. The studies described in this manuscript suggest that sensitivity of ovine myogenic cells to the antiproliferative effect of TGF-beta may vary with the stage of development.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Animal Science, University of Minnesota, St. Paul 55108.
authors.forename: M R
authors.initials: MR
authors.lastname: Hathaway
authors.forename: M S
authors.initials: MS
authors.lastname: Pampusch
authors.forename: J R
authors.initials: JR
authors.lastname: Hembree
authors.forename: W R
authors.initials: WR
authors.lastname: Dayton
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D017077:Culture Media, Conditioned; D005333:Fetus / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D018482:Muscle, Skeletal / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D012756:Sheep / Q000196:embryology / Q000254:growth & development*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D017077:Culture Media, Conditioned; D016212:Transforming Growth Factor beta