M13 DNA fingerprinting in differentiation of marine Vibrio species.
The identification and differentiation of bacterial strains and species are frequently carried out by the use of diagnostic biochemical profiles, serology, and the detection of restriction fragment length polymorphisms in genomic DNA. We show here that DNA restriction fragment length polymorphisms detected using a probe derived from bacteriophage M13 can discriminate between several marine Vibrio species. We have also demonstrated that individual isolates of Vibrio species can be differentiated using the M13 probe.
Chikarmane
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H
Marine Biological Laboratory, Woods Hole, Massachusetts 02543.
Bullis
R A
RA
Halvorson
H O
HO
D000818:Animals; D001048:Aplysia / Q000382:microbiology; D017104:Bacteriophage M13 / Q000235:genetics*; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D014733:Vibrio / Q000145:classification / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D004279:DNA, Viral