Determination of alprazolam and its major metabolites in serum microsamples by high-performance liquid chromatography and its application to pharmacokinetics in rats.
A high-performance liquid chromatographic method using a single-solvent extraction step is described for quantitating alprazolam and its metabolites in rat serum microsamples (50 microliters). The separation used a 2-mm I.D. reversed-phase Ultrasphere C18 column with a mobile phase of methanol-acetonitrile-sodium acetate buffer. By decreasing the methanol content in the mobile phase, triazolam and its metabolites can be extracted and separated using the same method. The detection limit was 5 ng/ml for all the compounds using an ultraviolet detector at 230 nm. The method showed the effect of aging on alprazolam pharmacokinetics following 0.8 mg/kg intravenous bolus alprazolam administration.
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Department of Psychology, Rutgers University, New Brunswick, NJ 08903.
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D000085:Acetates; D019342:Acetic Acid; D000097:Acetonitriles; D000375:Aging / Q000378:metabolism; D000525:Alprazolam / Q000097:blood* / Q000378:metabolism*; D000818:Animals; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000432:Methanol; D051381:Rats
D000085:Acetates; D000097:Acetonitriles; D002021:Buffers; D019342:Acetic Acid; D000432:Methanol; D000525:Alprazolam; C032159:acetonitrile