Direct characterization of bovine microsatellites from cosmids: polymorphism and synteny mapping.
A (TG)8 oligonucleotide probe was used to screen 186 cosmids from a commercial bovine cosmid library. Of the 56 positive discovered, 7 were sequenced in the region of the microsatellite and analysed for polymorphism. These microsatellites, IDVGA-2, -3, -7, -8, -9, -10, -11 showed the following number of alleles and polymorphism information content (PIC) values (7/0.616, 8/0.693, 6/0.641, 5/0.643, 2/0.239, 10/0.844, 6/0.720). The microsatellites were also assigned to synteny groups as follows: IDVGA-2/U17, IDVGA-3/U16, IDVGA-7/U7, IDVGA-8/U29, IDVGA-9/U3, IDVGA-10/U19, IDVGA-11/A (probably U18).
Ferretti
L
L
Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, CNR, Milano, Italy.
Leone
P
P
Pilla
F
F
Zhang
Y
Y
Nocart
M
M
Guérin
G
G
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary*; D003360:Cosmids; D017931:DNA Primers; D004276:DNA, Satellite; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D011110:Polymorphism, Genetic
D017931:DNA Primers; D004276:DNA, Satellite