Effects of bradykinin receptor agonists, and captopril and thiorphan in ferret isolated trachea: evidence for bradykinin generation in vitro.
Bradykinin B2 receptor agonists, but not a B1 receptor agonist, were potent spasmogens of ferret isolated trachea. Bradykinin-induced contractions were unaffected by several pharmacological agents, indicating a direct effect on airway smooth muscle B2 receptors. Captopril plus thiorphan caused contractions in approximately 70% of preparations that were abolished by B2 receptor antagonists, but not by B1 receptor antagonist. Thus, ferret tracheal tissues appear capable of releasing and degrading kinins in vitro. Inhibition of peptide degradation with captopril and thiorphan may allow the endogenous kinins to accumulate in concentrations sufficient to elicit tracheal contraction via activation of B2 receptors.
Farmer
S G
SG
Zeneca Pharmaceuticals Group, Wilmington, DE 19897-2300.
Broom
T
T
DeSiato
M A
MA
D000818:Animals; D001920:Bradykinin / Q000096:biosynthesis*; D065168:Bradykinin Receptor Antagonists; D002216:Captopril / Q000494:pharmacology*; D005289:Ferrets; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D018002:Receptors, Bradykinin / Q000819:agonists*; D015244:Thiorphan / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism*
D065168:Bradykinin Receptor Antagonists; D018002:Receptors, Bradykinin; D002216:Captopril; D015244:Thiorphan; D001920:Bradykinin