Xenotransplantation: an examination of the adhesive interactions between human lymphocytes and porcine renal epithelial cells.
Specific adhesion molecules stabilize the binding between lymphocytes and antigen bearing cells; this intercellular adhesion is vital to both the affector and effector phases of an immune response. It is not known whether adhesion molecules and their counter-receptors can form the cross-species interactions that will facilitate human T cell recognition of xenogeneic porcine target cells. In this report it is demonstrated that a higher proportion of mitogen-activated than of resting human lymphocytes adhere to cultured porcine renal epithelial cells. Furthermore, antibody blocking experiments demonstrated that at least part of this cell-cell binding is stabilized by the human adhesion molecules LFA-1 (lymphocyte function-associated antigen-1) and the alpha 4-containing integrins. It is possible that this capacity for cross-species adhesion will play a role during the cell-mediated rejection of clinical porcine xenografts.
Pleass
H C
HC
Department of Surgery, University of Newcastle upon Tyne, UK.
Forsythe
J L
JL
Proud
G
G
Taylor
R M
RM
Kirby
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D018801:CD2 Antigens / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000276:immunology*; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D006801:Humans; D039441:Integrin alpha4; D016023:Integrins / Q000276:immunology; D007668:Kidney / Q000166:cytology / Q000276:immunology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D013552:Swine / Q000276:immunology; D014183:Transplantation, Heterologous / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018801:CD2 Antigens; D015815:Cell Adhesion Molecules; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D039441:Integrin alpha4