Regulation of low density lipoprotein receptor activity in Chinese hamster ovary cells transfected with the c-fms gene.
Chinese hamster ovary (CHO) cells were transfected with the human c-fms gene, which encodes the receptor for macrophage colony-stimulating factor, to examine the effects on the low density lipoprotein (LDL) receptor activity. Degradation of [125I]LDL was significantly reduced in the transfected CHO cells as compared to non-transfected cells when incubated in lipoprotein-deficient serum. Quantitative analysis of LDL receptor mRNA using a competitive PCR method demonstrated that LDL receptor suppression occurred at transcription. These findings suggest that expression of the c-fms gene is involved in the regulation of LDL receptors on macrophages.
Yamamoto
K
K
Third Department of Internal Medicine, University of Tokyo, Japan.
Shimano
H
H
Inaba
T
T
Gotoda
T
T
Harada
K
K
Shimada
M
M
Watanabe
Y
Y
Osuga
J
J
Kawamura
M
M
Yazaki
Y
Y
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers; D005786:Gene Expression Regulation; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics*; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D017931:DNA Primers; D012333:RNA, Messenger; D011973:Receptors, LDL; D016173:Macrophage Colony-Stimulating Factor