title: Iodide induced lymphocytic thyroiditis in the BB/W rat: evidence of direct toxic effects of iodide on thyroid subcellular structure.
abstract: A high dietary iodine intake accelerates the development of lymphocytic thyroiditis (LT) in the BB/W rat. Our previous studies have defined the temporal sequence of the immunological events triggered by excess iodide intake in these animals. It was still not clear, however, whether these observed immunological changes were a direct effect on immune effector cells, or whether they represented a secondary response to a toxic effect of iodine on thyroid tissue. In the present study, the effect of excessive iodine intake on the subcellular structure of the BB/W rat thyroid gland, particularly, whether iodide had a toxic effect independent of its immune response has been examined. BB/W rats were exposed, prenatally through maternal drinking water, to excessive iodide at two doses (Moderate 3 x 10(-6) M iodide/l; High 3 x 10(-3) M iodide/l); a third group of BB/W rats was given tap water; till 12 weeks postnatal age. Two groups of Wistar rats received high dose iodide water or tap water for the same period of time and served as controls. Thyroid gland ultrastructure was determined by electron microscopic (EM) examination. Thyroid 125I uptake and perchlorate discharge tests were also performed in separate experiments. We found that thyroid glands of non-iodine supplemented Wistar rats were morphlogically normal under EM. There were no overt changes in the iodide treated Wistar rats. By contrast, iodide treated BB/W rats exhibited marked accumulation of secondary lysosomes and lipid droplets; markedly swollen and disrupted mitochondria and extreme dilatation of rough endoplasmic reticulum (RER).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Clinical Endocrinology, Westmead Hospital, NSW, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: S C
authors.initials: SC
authors.lastname: Boyages
mesh_terms: D000818:Animals; D007455:Iodine / Q000506:poisoning*; D008854:Microscopy, Electron; D051381:Rats; D011913:Rats, Inbred BB; D017208:Rats, Wistar; D013961:Thyroid Gland / Q000187:drug effects* / Q000648:ultrastructure*; D013967:Thyroiditis, Autoimmune / Q000139:chemically induced* / Q000473:pathology*
chemical_list: D007455:Iodine