title: Preparation of monoclonal antibodies for immunoblotting human growth hormone receptor and growth hormone-binding protein.
abstract: Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal antibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive against hGHRs from human IM-9 cells. The lower limit of detection for immunoblotting using this mAb was about 200 pg hGHR. The GHRP2-88 antibody also reacted with deglycosylated hGHRs from tunicamycin-treated IM-9 cells and with the growth hormone-binding protein (GH-BP) in human plasma.
authors.affiliation: Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Yamazaki
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Ikebuchi
authors.forename: A
authors.initials: A
authors.lastname: Asakura
authors.forename: Y
authors.initials: Y
authors.lastname: Ota
authors.forename: J
authors.initials: J
authors.lastname: Sawada
authors.forename: T
authors.initials: T
authors.lastname: Terao
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D006801:Humans; D015151:Immunoblotting; D011986:Receptors, Somatotropin / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D011986:Receptors, Somatotropin; C051815:somatotropin-binding protein