Immunoperoxidase procedures for rapid detection of bovine viral diarrhea-mucosal disease virus antigen.
Bovine viral diarrhea-mucosal disease (BVD-MD) virus antigen was detected in bovine fetal muscular cells from sera of 42 cattle persistently infected with noncytopathic BVD-MD virus by the indirect immunoperoxidase procedure (IIP) but not from sera of 100 apparently healthy cattle. These findings agreed with the results obtained by the interference method. Furthermore, BVD-MD virus antigens were detectable in the smears of buffy coats by the IIP procedure and in the formalin-fixed tissue sections by the avidin-biotin-peroxidase complex procedure. These procedures are therefore expected to be useful for rapid detection of BVD-MD virus antigen.
Saino
H
H
Abashiri Livestock Hygiene Service Center, Hokkaido, Japan.
Watanabe
H
H
Ikehata
T
T
D000818:Animals; D000956:Antigens, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis / Q000821:virology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D007564:Japan; D007962:Leukocytes / Q000821:virology
D000956:Antigens, Viral