Immobilization of heparin on polyacrylamide derivatives.
Heparin was coupled via its carboxyl group with a polyacrylamide derivative containing covalently bound amino groups using the carbodiimide reaction. Heparin immobilized in this way proved to be useful as an affinity carrier for the isolation of antithrombin III and heparin-binding proteins from boar seminal plasma.
Tichá
M
M
Department of Biochemistry, Charles University, Prague, Czech Republic.
Zelezná
B
B
Jonáková
V
V
Filka
K
K
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D000990:Antithrombin III / Q000302:isolation & purification; D002234:Carbodiimides / Q000737:chemistry; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006493:Heparin / Q000737:chemistry*; D008297:Male; D012661:Semen / Q000737:chemistry; D013552:Swine
D000180:Acrylic Resins; D002234:Carbodiimides; C016680:polyacrylamide gels; D000990:Antithrombin III; D006493:Heparin