Analysis of human T cell clones reactive with group V grass pollen allergens.
Twenty-seven T cell clones (TCC) reactive with group V allergens of Phleum pratense (Phlp V) were established from the peripheral blood of 3 patients allergic to grass pollen. Twenty-four TCC showed the helper cell phenotype CD3+, CD4+, CD8-; the remaining clones were CD3+CD4-CD8+. T cell recognition of Phlp V was exclusively HLA-DR restricted. Many of the Phlp V reactive TCC (19 of 27; 70%) were stimulated additionally by other group V allergens isolated from Lolium perenne, Poa pratensis, and Dactylis glomerata. These data indicate the existence of cross-reacting T cell epitopes among group V allergens of different grasses. The Phlp V triggered cytokine production demonstrated in 13 out of 24 CD4+ TCC a Th2-like pattern (high interleukin 4/gamma-interferon ratios) suggesting group V allergens as important targets of grass pollen specific IgE.
Müller
W D
WD
Abteilung für Klinische Immunologie, Friedrich-Schiller-Universität, Jena, Deutschland.
Karamfilov
T
T
Fahlbusch
B
B
Vogelsang
H
H
Jäger
L
L
D000328:Adult; D000485:Allergens / Q000276:immunology*; D002999:Clone Cells; D003429:Cross Reactions; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis; D008297:Male; D010641:Phenotype; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000485:Allergens; D016207:Cytokines; D007073:Immunoglobulin E