Application of column-switching techniques to the determination of medium polarity drugs: determination of acetazolamide in urine.
A column-switching system for the determination of the medium polarity diuretic acetazolamide in urine, has been designed. An Hypersil ODS C18, 30 microns (20 x 2.1 mm I.D.) pre-column was used for the pre-concentration and separation of acetazolamide from the biological matrix. The most polar urinary compounds were removed by washing the pre-column with a phosphate buffer solution (pH 3), and the fraction of eluate containing the analyte was switched to a LiChrospher RP C18, 5 microns (125 x 4 mm I.D.) analytical column, where it was chromatographed using gradient elution with acetonitrile-water, and detected at 275 nm. The most apolar urinary compounds were directly discarded by means of a second switching valve. Under these conditions the recovery of drug was 96 +/- 5% in the 0.50-100.0 micrograms/ml concentration range. The limit of detection was 10 ng/ml, the total analysis time being less than 8 min.
Campíns-Falcó
P
P
Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Spain.
Herráez-Hernández
R
R
Sevillano-Cabeza
A
A
D000086:Acetazolamide / Q000493:pharmacokinetics / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D012680:Sensitivity and Specificity
D000086:Acetazolamide