Pharmacokinetics, plasma protein binding and urinary excretion of N omega-nitro-L-arginine in rats.
The in vivo disposition of N omega-nitro-L-arginine (L-NOARG) has been examined in rats. Plasma concentrations of L-NOARG following an intravenous dose of 10 mg kg-1 were determined by high-performance liquid chromatography. Plasma L-NOARG concentrations declined biexponentially, with average half-lives of 11 min and 20 h. L-NOARG clearance did not appear to exhibit concentration-dependency below a plasma concentration of 4.56 x 10(-4) M (100 mg l-1). Ultrafiltration studies revealed insignificant binding of L-NOARG to rat plasma proteins. Urinary excretion of unchanged L-NOARG was minimal. These pharmacokinetic information may be useful in the design of in vivo experiments involving L-NOARG, as well as in the interpretation of the pharmacodynamics of this important nitric oxide synthase inhibitor.
Tabrizi-Fard
M A
MA
Department of Pharmaceutics, School of Pharmacy, State University of New York at Buffalo 14260.
Fung
H L
HL
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D008954:Models, Biological; D019335:Nitroarginine; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D014462:Ultrafiltration
D001798:Blood Proteins; D019335:Nitroarginine; D001120:Arginine