Bidirectional promoter of human monoamine oxidase A (MAO A) controlled by transcription factor Sp1.
The core promoter region of human monoamine oxidase (MAO) A has been identified in the two 90 bp repeat sequences, which can be further divided into four imperfect tandem repeats, each containing an Sp 1 binding site in the reversed orientation. Gel retardation and DNase 1 footprinting assays identified Sp 1 to be the major transcription factor binding to MAO A core promoter. In addition, positive association has been observed between cellular Sp1 concentration and MAO A promoter or catalytic activity, indicating that Sp1 is a controlling factor for human MAO A expression. DNA fragments from MAO A core promoter exhibit promoter activity in both orientations in a transient transfection assay, using human growth hormone as the reporter gene. A DNA probe isolated from upstream of the core promoter detected positive signals in a Northern analysis, suggesting that the reverse promoter activity may endogenously transcribe a new gene located upstream of MAO A.
Zhu
Q S
QS
Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles 90033.
Chen
K
K
Shih
J C
JC
D001483:Base Sequence; D001665:Binding Sites; D002384:Catalysis; D006801:Humans; D008969:Molecular Sequence Data; D008995:Monoamine Oxidase / Q000235:genetics* / Q000378:metabolism; D015345:Oligonucleotide Probes / Q000235:genetics; D009994:Osmolar Concentration; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016329:Sp1 Transcription Factor / Q000378:metabolism / Q000502:physiology*
D015345:Oligonucleotide Probes; D016329:Sp1 Transcription Factor; D008995:Monoamine Oxidase