A luciferase expression system for Physarum that facilitates analysis of regulatory elements.
We have developed a transient expression system for the protist Physarum polycephalum based on firefly luciferase. We demonstrate the utility of this system for comparing the activities of different promoters in Physarum amoebae, and also for detecting genetic elements that affect the level of gene expression. This system is likely to facilitate improvements in the stable transformation of this organism.
Bailey
J
J
Genetics Department, University of Leicester, UK.
Benard
M
M
Burland
T G
TG
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D001517:Coleoptera; D018274:Electroporation; D015870:Gene Expression; D007700:Kinetics; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D016839:Physarum polycephalum / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping
D011994:Recombinant Proteins; D008156:Luciferases