title: Effect of oxygen abstraction on the peroxidase-luminol-perborate system: relevance to the HRP enhanced chemiluminescence mechanism.
abstract: Abstraction of oxygen from the HRP enhanced chemiluminescence system has no significant effect on the chemiluminescence generated. It is, therefore, proposed that in the peroxidase-luminol-perborate system at pH 7.3, chemiluminescence is generated by a direct reaction of diazaquinones with hydrogen peroxide and not, as generally assumed, from the reaction of luminol radicals with the molecular oxygen.
authors.affiliation: Beckman Instruments Inc., Diagnostic Systems Group, Brea, California.
authors.forename: B
authors.initials: B
authors.lastname: Cercek
authors.forename: B
authors.initials: B
authors.lastname: Cercek
authors.forename: K
authors.initials: K
authors.lastname: Roby
authors.forename: L
authors.initials: L
authors.lastname: Cercek
mesh_terms: D001881:Borates; D005609:Free Radicals; D006735:Horseradish Peroxidase; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D008722:Methods; D010100:Oxygen; D013997:Time Factors
chemical_list: D001881:Borates; D005609:Free Radicals; D007202:Indicators and Reagents; D008165:Luminol; C010634:sodium borate; D006735:Horseradish Peroxidase; D010100:Oxygen