Volatile carbonyl levels in tissues of transgenic mice with nerve sheath tumors.
Volatile carbonyl compounds in homogenates prepared from various tissues of tumor-bearing transgenic mice were determined. Formaldehyde and acetaldehyde were derivatized to thiazolidines. Malonaldehyde was derivatized to 1-methylpyrazole. The derivatives were quantified by gas chromatography with a highly sensitive and specific nitrogen-phosphorus detector. The limits of quantitation of formaldehyde and malonaldehyde were 2 micrograms/ml of homogenate and 27 ng/ml of homogenate, respectively. Levels of malonaldehyde in the erythrocytes and gastrocnemius of tumor-bearing transgenic mice were elevated as compared to the same tissue in control non-transgenic mice. Brain, liver, kidney, heart, and spleen tissues of the tumor-bearing mice exhibited decreased malonaldehyde levels. Similar results were obtained for formaldehyde and acetaldehyde.
Ebeler
S E
SE
Department of Environmental Toxicology, University of California, Davis 95616.
Hinrichs
S H
SH
Clifford
A J
AJ
Shibamoto
T
T
D000079:Acetaldehyde / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005557:Formaldehyde / Q000032:analysis* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009132:Muscles / Q000378:metabolism; D018317:Nerve Sheath Neoplasms / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014018:Tissue Distribution; D014835:Volatilization
D017392:Thiobarbituric Acid Reactive Substances; D005557:Formaldehyde; D008315:Malondialdehyde; D000079:Acetaldehyde