Day time and night time effects of 24R,25-dihydroxycholecalciferol on renal alkaline phosphatase and gamma-glutamyltransferase activities in intact rats.
The effects of 24R,25-dihydroxycholecalciferol [24,25(OH)2 CC] on alkaline phosphatase (APA), gamma-glutamyltransferase (gamma-GT) and acid phosphatase (AP) activities were investigated on renal cortex slices of intact rats killed at 18h00 or at 06h00. At 06h00, three, six and nine hours after a single intraperitoneal injection of 24,25(OH)2 CC, APA activity was increased by 30%, 51% and 29%, respectively and gamma-GT activity, by 25%, 39% and 38%, as compared to their controls. AP on the other hand was not modified at all. These enzymatic stimulations were statistically higher than those measured at 18h00, respectively APA: 11%, 25%, 28% and gamma-GT: 2%, 20%, 22%. They can be explained by variations in new protein synthesis in relation with rat activity periods. Physiological significance of these renal effects remains to be elaborated.
Thanh
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XD
Laboratoire de Biochimie/C.H.U./Physiologie, U.F.R. Sciences Médicales/Pharmaceutiques, Angers.
Boisnard
P
P
Khan
M A
MA
Girault
A
A
D015650:24,25-Dihydroxyvitamin D 3 / Q000008:administration & dosage* / Q000494:pharmacology; D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002940:Circadian Rhythm; D005260:Female; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D015650:24,25-Dihydroxyvitamin D 3; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase