Induction of T cell tolerance by pretreatment with anti-ICAM-1 and anti-lymphocyte function-associated antigen-1 antibodies prevents antigen-induced eosinophil recruitment into the mouse airways.
To determine whether the induction of Th2 cell tolerance down-regulates Ag-induced eosinophil recruitment into the tissue, we studied the effect of the in vivo pretreatment with anti-ICAM-1 mAb, anti-LFA-1 mAb, and a soluble Ag on Ag-induced eosinophil infiltration in the trachea of sensitized mice. The in vivo pretreatment with anti-ICAM-1 mAb, anti-LFA-1 mAb, and a soluble Ag inhibited Ag-induced eosinophil infiltration in the mouse trachea in an Ag-specific manner. The pretreatment with anti-LFA-1 mAb and the Ag also had a weak inhibitory effect on the Ag-induced eosinophil infiltration. In contrast, the pretreatment with Ag alone, the combination of anti-ICAM-1 mAb and anti-LFA-1 mAb alone, or the combination of anti-ICAM-1 mAb and the Ag had no significant effect. In addition, the pretreatment with the two mAb and the Ag and, to a lesser degree, the pretreatment with anti-LFA-1 mAb and the Ag also prevented Ag-specific IgE production in mice. In vitro Ag-induced IL-2, IL-4, and IL-5 production was decreased in spleen cells of the mice pretreated with anti-ICAM-1 mAb, anti-LFA-1 mAb, and the Ag, but not with Ag alone or with the combination of anti-ICAM-1 mAb and anti-LFA-1 mAb alone, indicating the induction of both Th1 and Th2 cell tolerance in vivo. These results indicate that the induction of Th2 cell tolerance prevents Ag-induced eosinophil recruitment into the tissue and IgE Ab production and that ICAM-1/LFA-1 interaction is involved as a costimulatory signal in inducing T cell tolerance to a soluble Ag.
Nakao
A
A
Department of Internal Medicine, Chiba University School of Medicine, Japan.
Nakajima
H
H
Tomioka
H
H
Nishimura
T
T
Iwamoto
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D007108:Immune Tolerance / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology; D014132:Trachea / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D007376:Interleukin-2; D015848:Interleukin-5; D007378:Interleukins; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D015847:Interleukin-4; D007073:Immunoglobulin E