High-performance liquid chromatographic method for the separation of chlorambucil and its N-oxide prodrug.
A reversed-phase high-performance liquid chromatographic method is described to distinguish chlorambucil N-oxide from the parent chlorambucil and quantitate both after separation from biological samples. The influence of solvent pH, alcohol, acid and ion-pairing agent on the separation is described. The stability of chlorambucil and its N-oxide in buffers and alcohols, as well as stability during filtration is discussed with potential application for metabolic studies.
Chandler
K J
KJ
Department of Chemistry, University of Regina, Sask, Canada.
McCabe
J B
JB
Kirkpatrick
D L
DL
D000097:Acetonitriles; D000438:Alcohols; D000818:Animals; D002021:Buffers; D002699:Chlorambucil / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003497:Cyclic N-Oxides / Q000302:isolation & purification*; D004355:Drug Stability; D005021:Ethylamines; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D008862:Microsomes, Liver / Q000737:chemistry; D011355:Prodrugs / Q000302:isolation & purification; D051381:Rats; D014269:Trifluoroacetic Acid
D000097:Acetonitriles; D000438:Alcohols; D002021:Buffers; D003497:Cyclic N-Oxides; D005021:Ethylamines; D011355:Prodrugs; D002699:Chlorambucil; C087810:chlorambucil N-oxide; D014269:Trifluoroacetic Acid; C016162:triethylamine; D000432:Methanol; C032159:acetonitrile