Effect of minoxidil sulfate and pinacidil on single potassium channel current in cultured human outer root sheath cells and dermal papilla cells.
Minoxidil sulfate and pinacidil are K channel openers and are considered to promote hair growth. However, there have been no studies on the single channel current of isolated cells from hair follicles. Therefore, we characterized the single K channel current of outer root sheath cells and dermal papilla cells and the effect of K channel openers on K currents by patch clamp. We also carried out 86Rb efflux studies to observe macroscopic K channel currents. In physiological saline, these two cells showed two types of K channels, large and small conductance Ca(2+)-activated K channels, both intact cell-attached and excised inside-out patches. In symmetrical 150 mM K solution, unitary conductances were 246 and 70 pS, respectively. Intracellular ATP (up to 5 mM) or glibenclamide (20 nM), a specific ATP-sensitive K channel blocker, did not block these channels. Minoxidil sulfate (5 micrograms/ml) or pinacidil (10 microM) did not open these two types of K channels or increase 86Rb efflux. These results suggest that minoxidil sulfate or pinacidil did not activate K channel current in hair follicles, and that the drug effect on hair growth might be mediated by other mechanisms such as increased blood flow.
Nakaya
Y
Y
Department of Dermatology, Tokushima University, Japan.
Hamaoka
H
H
Kato
S
S
Arase
S
S
D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured; D005905:Glyburide / Q000494:pharmacology; D006146:Guanidines / Q000494:pharmacology*; D006197:Hair / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D006801:Humans; D008914:Minoxidil / Q000031:analogs & derivatives* / Q000494:pharmacology; D020110:Pinacidil; D015221:Potassium Channels / Q000187:drug effects* / Q000378:metabolism; D012413:Rubidium / Q000493:pharmacokinetics
D006146:Guanidines; D015221:Potassium Channels; C039516:minoxidil sulfate ester; D008914:Minoxidil; D020110:Pinacidil; D012413:Rubidium; D005905:Glyburide; D002118:Calcium