Potential use of IgG2-ELISA in the diagnosis of chronic elephantiasis and IgG4-ELISA in the follow-up of microfilaraemic patients infected with Brugia malayi.
Sera from fifty subjects with different presentations of Brugian filariasis and from common soil-transmitted helminth infections were tested for specific anti-filarial IgG and its subclasses. Anti-filarial IgG, IgG1 and IgG3 showed cross-reactivities with soil-transmitted helminthic infections and no significant differences in optical densities among the various groups of filarial patients. In comparison with other groups of subjects, IgG4-ELISA of sera from microfilaraemic patients and some previously microfilaraemic patients showed a significant increase in optical density readings, while IgG2-ELISA showed elevated optical density readings in sera of patients with chronic elephantiasis. Therefore IgG2-ELISA is potentially useful in the diagnosis of brugian chronic elephantiasis while IgG4-ELISA may be beneficial for follow-up diagnosis of treated microfilaraemic patients.
Rahmah
N
N
Dept. of Microbiol. and Parasitol., School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan.
Anuar
A K
AK
Karim
R
R
Mehdi
R
R
Sinniah
B
B
Omar
A W
AW
D000818:Animals; D000909:Antibodies, Helminth / Q000145:classification / Q000276:immunology*; D017178:Brugia malayi / Q000276:immunology*; D002908:Chronic Disease; D004605:Elephantiasis, Filarial / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005500:Follow-Up Studies; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification / Q000276:immunology*
D000909:Antibodies, Helminth; D007074:Immunoglobulin G