Enhanced cholesterol esterase activity in the pancreas of rats with streptozotocin-induced diabetes.
Diabetes was induced in rats by the intravenous administration of streptozotocin (STZ; 60 mg/ml). The activity of cholesterol esterase (CEase) in various tissues was determined by use of either a fluorogenic or radioactive substrate. Significant CEase activity was detected in extracts of pancreas. The specific activity of pancreatic CEase was considerably greater in pancreatic extracts from diabetic rats compared with normal rats. The activity of pancreatic CEase increased 6 d after the injection of STZ, but the difference was not statistically significant. It reached a maximum value approximately twice that of normal pancreas at 30 d with statistic significance. The highest specific activity of pancreatic CEase was found in the cytosolic fraction from diabetic rats, whereas the specific activity of the enzyme was lowest the same fraction from normal rats.
Oshima
G
G
School of Pharmaceutical Science, Kitasato University, Tokyo, Japan.
Nakagawa
Y
Y
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D007422:Intestines / Q000201:enzymology; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D002787:Sterol Esterase / Q000378:metabolism*; D013311:Streptozocin
D013311:Streptozocin; D002787:Sterol Esterase