Stereochemistry of hydrogen addition to C-25 of desmosterol by sterol-delta 24-reductase of rat liver homogenate.
Chemically synthesized (26)- and (27)-methyl-13C-labeled desmosterol (3, 5) were separately incubated with rat liver homogenate. 13C-NMR analysis of the incubation products indicated that the C-25 hydrogen of cholesterol was introduced from the si-face of 3 and the re-face of 5.
Yagi
T
T
Kyoritsu College of Pharmacy, Tokyo, Japan.
Kobayashi
N
N
Morisaki
M
M
Hara
N
N
Fujimoto
Y
Y
D000818:Animals; D003897:Desmosterol / Q000737:chemistry / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D003897:Desmosterol; D006859:Hydrogen; D010088:Oxidoreductases; C022673:delta 24-sterol reductase