title: The secretion of alpha-MSH from xenopus melanotropes involves calcium influx through omega-conotoxin-sensitive voltage-operated calcium channels.
abstract: The secretory activity of endocrine cells largely depends on the concentration of free cytosolic calcium. We have studied the mechanisms that are involved in supplying the calcium necessary for the secretion of alpha-melanophore-stimulating hormone (alpha-MSH) from melanotrope cells in the pituitary intermediate lobe of the amphibian Xenopus laevis. Using whole-cell voltage clamp, high-voltage activated calcium currents were observed, with a peak current between 0 and +20 mV. Two types of Ca(2+)-currents appeared, depending on the experimental setup. An inactivating current, which was observed after a 10 msec depolarizing prepulse, resembled currents through N-type channels as it was clearly inhibited by 1 microM omega-conotoxin. The second type was a non-inactivating current, which was blocked up to 50% by 1 microM nifedipine, indicating its L-type nature. Only a small component of this inactivating current could be blocked by omega-conotoxin. No evidence was found for the presence of transient, low-voltage activated currents. The spontaneous secretion of alpha-MSH from superfused neurointermediate lobes was dependent on extracellular calcium, as low calcium conditions (10(-4)-10(-8) M) rapidly inhibited this process. Under these conditions, secretion was not affected by depolarizing concentrations of potassium chloride. The calcium ionophore A23187 increased secretion under low calcium conditions, but had no effect on spontaneous alpha-MSH release. These results suggest that spontaneous alpha-MSH release depends on influx of calcium through voltage-operated calcium channels.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Cellular Animal Physiology, Nijmegen Institute for Neurosciences, The Netherlands.
authors.forename: W J
authors.initials: WJ
authors.lastname: Scheenen
authors.forename: H P
authors.initials: HP
authors.lastname: de Koning
authors.forename: B G
authors.initials: BG
authors.lastname: Jenks
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
authors.forename: E W
authors.initials: EW
authors.lastname: Roubos
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology*; D004553:Electric Conductivity; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000494:pharmacology*; D010902:Pituitary Gland / Q000378:metabolism*; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin; D014982:Xenopus laevis; D000521:alpha-MSH / Q000378:metabolism*; D020866:omega-Conotoxin GVIA
chemical_list: D015220:Calcium Channels; D010455:Peptides; D013729:Terpenes; D000001:Calcimycin; D000521:alpha-MSH; D019284:Thapsigargin; D020866:omega-Conotoxin GVIA; D002118:Calcium