title: Characterization of peroxidases in luminol chemiluminescence coupled with copper-catalysed oxidation of cysteamine.
abstract: Hydrogen peroxide formed during the course of the copper(II)-catalysed oxidation of cysteamine with oxygen was continuously determined by a peroxidase (POD)-catalysed luminol chemiluminescence (CL) method. Horseradish peroxidase (HRP), lactoperoxidase (LPO) and Arthromyces ramosus peroxidase (ARP) were used as a CL catalyst. The respective PODs gave specific CL intensity-time profiles. HRP caused a CL delay, and ARP gave a time-response curve which followed the production rate of H2O2. LPO gave only a weak CL flash which decayed promptly. These differences of CL response curves could be explained in terms of the different reactivities of PODs for superoxide anion and the different formation rate of luminol radicals in the peroxidation of luminol catalysed by POD.
authors.affiliation: Faculty of Engineering, Hokkaido University, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kamidate
authors.forename: A
authors.initials: A
authors.lastname: Katayama
authors.forename: H
authors.initials: H
authors.lastname: Ichihashi
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D003300:Copper; D003543:Cysteamine; D006735:Horseradish Peroxidase; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D008722:Methods; D010084:Oxidation-Reduction; D013997:Time Factors
chemical_list: D007202:Indicators and Reagents; D008165:Luminol; D003543:Cysteamine; D003300:Copper; D006735:Horseradish Peroxidase