[Hypomodified phenylalanine transfer RNAs (tRNA(Phe)) in uterine myoma tissue of women].
Phenol extracted crude tRNA samples obtained from uterine myoma tissue were additionally purified on DEAE-cellulose column and were finally fractionated on BD-cellulose column. Fractions eluted by ethanol gradient (concentration from 0% to 20%) were hybridized by dot blot technique with DNA probe which consisted of 15 nucleotides fragment complementary to 3' end of the tRNA(Phe). Five separate distinct peaks were found. According to genetic code rules restricted to two the number of tRNA(Phe) isoacceptors, it is assumed that three major peaks representing roughly 70% of total tRNA(Phe) pool, probably are hypomodified.
Baranowski
W
W
II Kliniki Ginekologii Operacyjnej AM w Lublinie.
Tomaszewski
J
J
D002848:Chromatography, DEAE-Cellulose; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000235:genetics*; D012334:RNA, Neoplasm / Q000032:analysis*; D012343:RNA, Transfer / Q000032:analysis*; D014594:Uterine Neoplasms / Q000235:genetics*
D012334:RNA, Neoplasm; D012343:RNA, Transfer
Hypomodyfikowane formy transportujacych RNA dla fenyloalaniny (tRNA(Phe)) w tkance mieśniaka macicy u kobiet.