A comparative histologic study of the pulp-dentinal interface in undemineralized and demineralized tooth sections.
This study examines cellular differences between undemineralized and demineralized tooth sections. The material comprised unerupted and partly erupted third molars. After fixation for 24 h, 10- to 15-microns ground sections were prepared from half of the tooth, using a cutting-grinding system. The sections were compared with demineralized routine sections from the other half of the tooth. Undemineralized sections showed a better capacity for preserving the tissue integration. Especially between the odontoblast layer and the predentin a low frequency of disjunctions or tears were noted. At this level of examination fixation quality and vacuole frequency (intra-cellular spaces) did not show any differences between the two methods.
Bjørndal
L
L
Department of Cariology and Endodontics, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Denmark.
Thylstrup
A
A
D002462:Cell Membrane / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure; D003648:Decalcification Technique; D003782:Dental Pulp / Q000166:cytology*; D003804:Dentin / Q000166:cytology*; D005110:Extracellular Space; D016591:Histocytological Preparation Techniques; D006801:Humans; D008964:Molar, Third / Q000166:cytology; D009804:Odontoblasts / Q000166:cytology; D016707:Tissue Fixation; D014078:Tooth Eruption; D014097:Tooth, Unerupted / Q000473:pathology; D014617:Vacuoles / Q000648:ultrastructure
D002843:Chromatin