The proliferating cell marker monoclonal antibody Ki-67 recognizes specific antigens associated with the nuclear envelope of the early Drosophila embryo.
Immunofluorescence and immunoelectron microscopy indicated that the antibody raised against the nuclear antigen Ki-67 of mammalian cells recognized antigenic determinants of early Drosophila embryos, localized on the outside of the nuclear envelope. Hence, the nuclear envelope of Drosophila appears to share a similar epitope with the chromosome scaffold of mitotic mammalian cells. With the progression of mitosis the antigen persisted around the mitotic spindle region and was also found in the pole regions at metaphase and anaphase. The antibody also stained the equatorial regions of the spindles from anaphase to late telophase. The antibody may therefore be used as a biochemical marker of the nuclear envelope for studying nuclear membrane biogenesis and behavior during the mitotic divisions of the Drosophila embryo.
Callaini
G
G
Department of Evolutionary Biology, University of Siena, Italy.
Riparbelli
M G
MG
Cintorino
M
M
Tripodi
S A
SA
Bianciardi
G
G
Tosi
P
P
Dallai
R
R
D000705:Anaphase; D000818:Animals; D000911:Antibodies, Monoclonal; D004331:Drosophila melanogaster / Q000196:embryology* / Q000276:immunology; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D008677:Metaphase; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008938:Mitosis; D009363:Neoplasm Proteins / Q000032:analysis*; D009685:Nuclear Envelope / Q000276:immunology*; D009687:Nuclear Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins