title: Parthenogenetic development of bovine oocytes matured in vitro for 24 hr and activated by ethanol and cycloheximide.
abstract: This research was undertaken to improve development of parthenogenetic embryos following various combined treatments of ethanol and cycloheximide. In Experiment 1 in vitro matured oocytes (IVM, 24 hr) were treated with 7% ethanol for 5 min followed by incubation in 10 micrograms/ml cycloheximide in Medium 199 for 0 (control), 5, 10, and 20 hr. Development to 2-8 cells following culture for 3 days was similar among treated groups (32-41%; P > 0.05), which was higher than that of controls (6%; P < 0.05). Experiment 2 compared pre-ethanol exposures for 0, 1, 2.5, and 5 min, followed by 5 hr cycloheximide treatment on activation development. One- to 5-min groups resulted in 42-44% cleavage contrasted to 1-12% for controls (P < 0.05). Experiment 3 examined the effect on oocyte development of ethanol and different concentrations of cycloheximide (0, 1, 5, and 10 micrograms/ml). Cleavage to 2-8 cells was similar among the 5 and 10 micrograms/ml cycloheximide groups (36% and 42%, P > 0.05) but lower (P < 0.05) for the 1 micrograms/ml group (24%) and the controls (2-13%). When 5 micrograms/ml cycloheximide was used (Experiment 4), pre-exposure to ethanol (1, 2.5, and 5 min) resulted in more oocytes cleaved (38-41%) than in the cycloheximide alone group (0%) or the control (0%, P < 0.05). Experiment 5 tested blastocyst development of the activated oocytes with or without cytochalasin B treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Animal Science, Cornell University, Ithaca, New York.
authors.forename: G A
authors.initials: GA
authors.lastname: Presicce
authors.forename: X
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D001755:Blastocyst; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D003513:Cycloheximide / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D004357:Drug Synergism; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects*; D010312:Parthenogenesis / Q000187:drug effects*
chemical_list: D003571:Cytochalasin B; D000431:Ethanol; D003513:Cycloheximide; D002118:Calcium