title: Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes.
abstract: Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression.
authors.affiliation: Department of Medical and Physiological Chemistry, Lund University, Sweden.
authors.forename: L S
authors.initials: LS
authors.lastname: Holst
authors.forename: A M
authors.initials: AM
authors.lastname: Hoffmann
authors.forename: H
authors.initials: H
authors.lastname: Mulder
authors.forename: F
authors.initials: F
authors.lastname: Sundler
authors.forename: C
authors.initials: C
authors.lastname: Holm
authors.forename: A
authors.initials: A
authors.lastname: Bergh
authors.forename: G
authors.initials: G
authors.lastname: Fredrikson
mesh_terms: D000818:Animals; D003456:Cryptorchidism / Q000201:enzymology / Q000473:pathology; D005260:Female; D017403:In Situ Hybridization; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology; D050356:Lipid Metabolism; D008297:Male; D008698:Mesylates / Q000633:toxicity; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000201:enzymology*; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000201:enzymology / Q000254:growth & development / Q000473:pathology
chemical_list: D008698:Mesylates; D002787:Sterol Esterase; C002994:ethylene dimethanesulfonate