title: Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4.
abstract: We constructed a yeast artificial chromosome (YAC) framework map of human chromosome 4 by screening a YAC library with 63 polymorphic DNA markers located on the chromosome. These genetic markers are from two framework meiotic maps that had previously been constructed by two research groups, and are placed on the two maps with odds for their order of 1000:1 or greater. In addition to isolating and determining the sizes of 141 YAC clones for 54 of these markers, we combined the two framework meiotic maps to produce a single integrated map. These combined maps and the YAC clones provide a set of extended DNA loci ordered at high odds that can be used to isolate additional polymorphic loci and genes, and to serve as a framework for obtaining a higher resolution physical map of the chromosome.
authors.affiliation: Department of Genetics, Stanford University, Palo Alto, CA 94304.
authors.forename: J B
authors.initials: JB
authors.lastname: Fan
authors.forename: J
authors.initials: J
authors.lastname: DeYoung
authors.forename: R
authors.initials: R
authors.lastname: Lagacé
authors.forename: R A
authors.initials: RA
authors.lastname: Lina
authors.forename: Z
authors.initials: Z
authors.lastname: Xu
authors.forename: J C
authors.initials: JC
authors.lastname: Murray
authors.forename: K H
authors.initials: KH
authors.lastname: Buetow
authors.forename: J
authors.initials: J
authors.lastname: Weissenbach
authors.forename: R D
authors.initials: RD
authors.lastname: Goold
authors.forename: D R
authors.initials: DR
authors.lastname: Cox
mesh_terms: D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002894:Chromosomes, Human, Pair 4; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D008540:Meiosis; D011110:Polymorphism, Genetic; D016324:Sequence Tagged Sites
chemical_list: D005819:Genetic Markers