[Synthesis and cloning of genes for the antisense peptides for human calcitonin and miniproinsulin].
Because of the potential significance of the 'molecular recognition' theory for studies in molecular biology and biotechnology, the theory is worth being examined using methods of chemical-enzymatic gene synthesis, recombinant DNA construction and microbiological peptide synthesis. We therefore undertook the synthesis of human Va18-calcitonin, miniproinsulin, and the corresponding antisense peptides as model compounds. In designing an experimental system the idea was to combine sense and antisense polypeptides into a single chain and to examine their intramolecular interaction. In this paper the chemical-enzymatic synthesis, cloning and expression of the genes for calcitonin, miniproinsulin, the corresponding antisense peptides and their combinations are described. The recombinant DNAs obtained were able to direct in vivo expression of the target polypeptides as hybrid proteins with the IgG-binding domain of the staphylococcal A protein in bacterial cells.
Efimov
V A
VA
Aronova
E A
EA
Buriakova
A A
AA
Kalinkina
A L
AL
Letunova
A B
AB
Fradkov
A F
AF
Chakhmakhcheva
O G
OG
D000595:Amino Acid Sequence; D001483:Base Sequence; D002116:Calcitonin / Q000138:chemical synthesis / Q000235:genetics*; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000235:genetics*; D011384:Proinsulin / Q000138:chemical synthesis / Q000235:genetics*
D004274:DNA, Recombinant; D010455:Peptides; C060793:miniproinsulin; D002116:Calcitonin; D011384:Proinsulin
Sintez i klonirovanie genov antismyslovykh peptidov kal'tsitonina i miniproinsulina cheloveka.