Density gradient separation of L-asparaginase-loaded human erythrocytes.
L-Asparaginase has been encapsulated in human red blood cells using a hypotonic dialysis process. Erythrocytes loaded with L-Asparaginase were separated into eight fractions using a discontinuous Percoll density gradient. A minor cell subpopulation of low density cells and a major subpopulation of denser erythrocytes was obtained after hypotonic dialysis treatment, in both the absence or presence of L-Asparaginase. The encapsulated L-Asparaginase activity per resealed erythrocyte was higher in low-density cells and decreased progressively with increasing in cellular density.
Garín
M I
MI
Laboratoire de Biopharmacologie Transfusionnelle, Centre Régional de Transfusion Sanguine, Tours, France.
Kravtzoff
R
R
Chestier
N
N
Sanz
S
S
Pinilla
M
M
Luque
J
J
Ropars
C
C
D001215:Asparaginase; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003956:Dialysis; D004339:Drug Compounding / Q000379:methods*; D004912:Erythrocytes; D006801:Humans; D007038:Hypotonic Solutions; D011205:Povidone; D012822:Silicon Dioxide
D007038:Hypotonic Solutions; C016039:Percoll; D012822:Silicon Dioxide; D001215:Asparaginase; D011205:Povidone