Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans.
A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 x 10(2) to 2.5 x 10(4) transformants per 10(6) viable protoplasts and microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants.
Moreno
M A
MA
Department de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Pascual
C
C
Gibello
A
A
Ferrer
S
S
Bos
C J
CJ
Debets
A J
AJ
Suárez
G
G
D001230:Aspergillus / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics*; D004261:DNA Replication; D004271:DNA, Fungal / Q000032:analysis; D008938:Mitosis; D010957:Plasmids / Q000235:genetics; D011523:Protoplasts; D014170:Transformation, Genetic
D004271:DNA, Fungal