Establishment of a pluripotent cell line derived from a medaka (Oryzias latipes) blastula embryo.
A pluripotent cell line, OLES1, was established from a blastula embryo of a small freshwater fish, medaka (Oryzias latipes). Cells of this cell line were small and round, and they grew actively and stably in culture as dense clusters. They exhibited a positive alkaline phosphatase activity upon histochemical staining. When the cells were treated with retinoic acid, differentiation into various types of cells, including melanocytes, dopa-positive precursors of melanocytes, and cells with a molecular marker of skeletal muscles, troponin T, was induced in vitro. The present study opens a way to establishing embryonic stem cell lines in fish.
Wakamatsu
Y
Y
Laboratory of Freshwater Fish Stocks, Nagoya University, Japan.
Ozato
K
K
Sasado
T
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers; D001756:Blastoderm / Q000166:cytology*; D002460:Cell Line; D004627:Embryonic Induction; D009990:Oryzias / Q000196:embryology; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology; D014212:Tretinoin; D014336:Troponin / Q000032:analysis; D020107:Troponin T
D015415:Biomarkers; D014336:Troponin; D020107:Troponin T; D014212:Tretinoin; D000469:Alkaline Phosphatase