Immunoreactive C-type natriuretic peptide in human adrenal glands and adrenal tumors.
C-type natriuretic peptide (CNP) in human adrenal glands and adrenal tumors was measured with a specific radioimmunoassay for CNP. Tissue immunoreactive (IR-) CNP concentrations were 0.54 +/- 0.40 pmol/g wet tissue (gwt) (mean +/- SD) in 14 pheochromocytomas, 0.69 +/- 0.19 pmol/gwt in six adrenocortical tumors, and 0.49 +/- 0.22 pmol/gwt in seven normal adrenal glands (cortex and medulla mixed). These concentrations were comparable to those found in tissues from human brains. Sephadex G-50 superfine column chromatography and reverse-phase high performance liquid chromatography revealed that IR-CNP in normal adrenal glands and pheochromocytoma consisted of at least two components: a component in low molecular weight form chromatographically identical to CNP-22 and the other, a high molecular weight form very similar to human CNP-53. This study has shown that IR-CNP is present in human adrenal glands and adrenal tumors with similar molecular forms and comparable concentrations to those in the human brain.
Totsune
K
K
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Takahashi
K
K
Murakami
O
O
Itoi
K
K
Sone
M
M
Ohneda
M
M
Satoh
F
F
Miura
Y
Y
Mouri
T
T
D000236:Adenoma / Q000737:chemistry / Q000276:immunology; D000310:Adrenal Gland Neoplasms / Q000737:chemistry* / Q000276:immunology; D000311:Adrenal Glands / Q000737:chemistry*; D000368:Aged; D009320:Atrial Natriuretic Factor / Q000276:immunology / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000302:isolation & purification*; D010673:Pheochromocytoma / Q000737:chemistry / Q000276:immunology; D011863:Radioimmunoassay
D009419:Nerve Tissue Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor