title: Sensitive method for measuring lactate dehydrogenase activity in human serum by differential-pulse polarography.
abstract: A sensitive and accurate differential-pulse polarographic method is reported for the measurement of lactate dehydrogenase (LDH) activity. The method is based on the polarographic measurement of the NAD+ formed via the enzymic reaction of LDH with pyruvate and NADH. The method was successfully applied to measure LDH activity in the serum of normal and diseased individuals.
authors.affiliation: Department of Chemistry, College of Science, University of Mosul, Iraq.
authors.forename: S T
authors.initials: ST
authors.lastname: Sulaiman
authors.forename: T S
authors.initials: TS
authors.lastname: al-Najafi
authors.forename: H S
authors.initials: HS
authors.lastname: Hamdon
mesh_terms: D004194:Disease; D006801:Humans; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D009243:NAD / Q000032:analysis; D011048:Polarography / Q000379:methods; D011773:Pyruvates; D012016:Reference Values; D012680:Sensitivity and Specificity
chemical_list: D007202:Indicators and Reagents; D011773:Pyruvates; D009243:NAD; D007770:L-Lactate Dehydrogenase