title: Differential effects of interleukin 1-alpha (IL-1 alpha) or tumor necrosis factor-alpha (TNF-alpha) on motility of human melanoma cell lines on fibronectin.
abstract: Interleukin-1 alpha (IL-1 alpha) and tumor necrosis factor-alpha (TNF-alpha) induce a motogenic response in a number of benign and malignant cells. We examined the chemokinetic effects of these cytokines on the cell migration of four melanoma cell lines on fibronectin using modified Boyden chambers and video-time lapse analysis. Flow cytometry analysis of IL-1 receptors, TNF receptors, and shifts in beta 1 integrin expression were correlated with the effects of these cytokines on cell migration on fibronectin. The four melanoma cell lines exhibited heterogeneous expression of types I and II IL-1 receptors as well as p60 TNF receptors. Scant p80 TNF receptor expression was detected on only one cell line. Three of four melanoma cell lines demonstrated type I IL-1 receptors by Western blotting. IL-1 alpha and TNF-alpha induced heterogeneous modulation of beta 1 integrin expression in the four melanoma cell lines tested; downward shift of the alpha 2, alpha 3, alpha 4, and beta 1 integrin subunits was detected among three of the melanoma cell lines as were upward shifts of the alpha 4, alpha 5, and alpha 6 integrin subunits among three of the melanoma cell lines. IL-1 alpha and TNF-alpha induced enhanced migration on fibronectin in one of the melanoma cell lines and were related to an upward shift in the alpha 4 and alpha 5 integrin subunit expression. Taken together, the findings indicate that expression of a particular receptor for IL-1 or TNF does not necessarily signal a motogenic response in melanoma cells, but induces heterogeneous shifts in beta 1 integrin expression. However, upregulation in alpha 4 and alpha 5 integrin subunits appears to relate to enhanced migration on fibronectin.
authors.affiliation: Department of Pathology, Harvard Medical School, Boston, Massachusetts.
authors.forename: S K
authors.initials: SK
authors.lastname: Dekker
authors.forename: J
authors.initials: J
authors.lastname: Vink
authors.forename: B J
authors.initials: BJ
authors.lastname: Vermeer
authors.forename: J A
authors.initials: JA
authors.lastname: Bruijn
authors.forename: M C
authors.initials: MC
authors.lastname: Mihm
authors.forename: H R
authors.initials: HR
authors.lastname: Byers
mesh_terms: D015153:Blotting, Western; D002465:Cell Movement / Q000502:physiology; D005353:Fibronectins; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008545:Melanoma / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D017472:Receptors, Interleukin-1 / Q000032:analysis / Q000502:physiology; D016029:Receptors, Very Late Antigen / Q000032:analysis / Q000502:physiology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D005353:Fibronectins; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D016029:Receptors, Very Late Antigen; D014409:Tumor Necrosis Factor-alpha