Inhibition of G1 cyclin expression and G1 cyclin-dependent protein kinases by cAMP in an astrocytic cell line.
The effects of cAMP on cell cycle progression were examined using an astrocytic cell line. We show that forskolin and 8-bromo-cAMP block the basic Fibroblast Growth Factor-induced DNA synthesis, do not inhibit mitogen activated protein kinase activation whereas they reduce G1 cyclin (E and D1) expression without modification of cyclin A level. Furthermore, they inhibit the activation of cyclin A- and cyclin E-dependent histone H1 kinases. These results suggest that cAMP may exert its antiproliferative effects through the regulation of cyclin synthesis and cyclin-dependent kinase activation.
Gagelin
C
C
Unité de recherche sur la glande thyroide et la regulation hormonale, U.96 INSERM, Kremlin-Bicêtre.
Pierre
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M
Toru-Delbauffe
D
D
D001253:Astrocytes / Q000166:cytology*; D042846:CDC2-CDC28 Kinases; D002455:Cell Division; D002460:Cell Line; D000242:Cyclic AMP / Q000502:physiology*; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016213:Cyclins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004789:Enzyme Activation; D007150:Immunohistochemistry; D007700:Kinetics; D019950:Mitogen-Activated Protein Kinase 1; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism
D016213:Cyclins; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; D019950:Mitogen-Activated Protein Kinase 1