title: A putative ATP-binding protein from the che/fla locus of Bacillus subtilis.
abstract: A majority of the chemotaxis and flagellar genes of Bacillus subtilis are found in the major che/fla operon which spans over 26 kilobases of DNA and encodes at least 30 genes. In this operon, a single open reading frame, designated orf298, has been demonstrated to encode a 33,131 Dalton protein which shows no evidence of being involved in chemotaxis or motility. A strain disrupted in orf298 was assayed for motility and chemotaxis and always behaved as the wild-type strain. Inspection of the translated sequence revealed a consensus ATP-binding region. While the role of ORF298 has not yet been determined, ATP-binding and/or hydrolysis is a likely function.
authors.affiliation: Department of Biochemistry, College of Medicine, University of Illinois, Urbana 61801.
authors.forename: M L
authors.initials: ML
authors.lastname: Kirsch
authors.forename: P B
authors.initials: PB
authors.lastname: Carpenter
authors.forename: G W
authors.initials: GW
authors.lastname: Ordal
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002633:Chemotaxis / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005407:Flagella / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames; D009876:Operon
chemical_list: C090796:ATP-binding protein, bacteria; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial