title: Immunoperoxidase procedures for rapid detection of bovine viral diarrhea-mucosal disease virus antigen.
abstract: Bovine viral diarrhea-mucosal disease (BVD-MD) virus antigen was detected in bovine fetal muscular cells from sera of 42 cattle persistently infected with noncytopathic BVD-MD virus by the indirect immunoperoxidase procedure (IIP) but not from sera of 100 apparently healthy cattle. These findings agreed with the results obtained by the interference method. Furthermore, BVD-MD virus antigens were detectable in the smears of buffy coats by the IIP procedure and in the formalin-fixed tissue sections by the avidin-biotin-peroxidase complex procedure. These procedures are therefore expected to be useful for rapid detection of BVD-MD virus antigen.
authors.affiliation: Abashiri Livestock Hygiene Service Center, Hokkaido, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Saino
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Ikehata
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis / Q000821:virology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D007564:Japan; D007962:Leukocytes / Q000821:virology
chemical_list: D000956:Antigens, Viral