Molecular analysis of mouse Rab11b: a new type of mammalian YPT/Rab protein.
Since the realization that the vesicular transport machinery is conserved from yeast to vertebrate neurons, much interest in the proteins involved has been generated. Here we describe a new type of mammalian YPT/Rab protein, named Rab11b, that is most abundantly expressed in brain, heart, and testis. It has all the hallmarks of a YPT3/Rab11 protein, but is more closely related to a ypt3-related gene isolated from fish (97% homology) than to the previously described four mammalian Rab11 genes (89% homology). The mammalian Rab11 genes discovered previously are, by contrast, 100% identical to each other. The genomic structure of a mammalian Rab11 protein is described for the first time. It is surprisingly asymmetrical, with 96% of the coding sequence contained in one-third of the transcription unit. Two copies of this gene are mapped to mouse chromosomes 1 and 17. The fine structural analysis of Rab11b and the protein sequence comparison provides a close look at all YPT3/Rab11-related genes cloned so far and clues to their possible relationship.
Lai
F
F
Department of Zoology, University of Texas at Austin 78712-1064.
Stubbs
L
L
Artzt
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D005810:Multigene Family; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
D004247:DNA; D005656:Fungal Proteins; D019204:GTP-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C075976:ypt3 protein, S pombe