Effect of oxygen abstraction on the peroxidase-luminol-perborate system: relevance to the HRP enhanced chemiluminescence mechanism.
Abstraction of oxygen from the HRP enhanced chemiluminescence system has no significant effect on the chemiluminescence generated. It is, therefore, proposed that in the peroxidase-luminol-perborate system at pH 7.3, chemiluminescence is generated by a direct reaction of diazaquinones with hydrogen peroxide and not, as generally assumed, from the reaction of luminol radicals with the molecular oxygen.
Cercek
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B
Beckman Instruments Inc., Diagnostic Systems Group, Brea, California.
Cercek
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B
Roby
K
K
Cercek
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L
D001881:Borates; D005609:Free Radicals; D006735:Horseradish Peroxidase; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D008722:Methods; D010100:Oxygen; D013997:Time Factors
D001881:Borates; D005609:Free Radicals; D007202:Indicators and Reagents; D008165:Luminol; C010634:sodium borate; D006735:Horseradish Peroxidase; D010100:Oxygen