Transforming growth factor beta 1, insulin and prostaglandin E1 enhance prostaglandin F2 alpha mitogenic action in Swiss 3T3 cells via separate events.
Transforming growth factor beta 1 (TGF beta 1) had no mitogenic effect in Swiss 3T3 cells, but could increase prostaglandin F2 alpha (PGF2 alpha)-induced DNA synthesis. Insulin, but not prostaglandin E1 (PGE1), further enhanced PGF2 alpha action at low TGF beta 1 concentrations. TGF beta 1 also acted concertedly with the protein kinase C (PKC) activator 1-oleoyl-2-acetylglycerol to induce mitogenesis. Thus, it appears that TGF beta 1 and insulin act via separate signals, while TGF beta 1 and PGE1 might share a common pathway not involving TGF beta 1-mediated prostaglandin synthesis. These results suggest that TGF beta 1 might elicit various signalling mechanisms to enhance PGF2 alpha-triggered events.
Gomez de Alzaga
M
M
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), Buenos Aires, Argentina.
Goin
M
M
Ortiz
M
M
Jimenez de Asua
L
L
D016475:3T3 Cells; D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D015237:Dinoprost / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D007328:Insulin; D008934:Mitogens; D016212:Transforming Growth Factor beta; D004247:DNA; D015237:Dinoprost; D000527:Alprostadil