title: Transfer of shuttle vectors containing Bacillus thuringiensis toxin gene into wild-type B. cereus, B. brevis and B. subtilis by electroporation.
abstract: Gram positive and negative bacterial shuttle vectors carrying Bacillus thuringiensis (B.t.) toxin gene were introduced by electroporation into wild-type Bacillus cereus, B. brevis and B. subtilis. The transformation efficiencies for these bacteria were around 10(1)-10(4) transformants per micrograms DNA based on the numbers of neomycin- and ampicillin-resistant colonies produced. The structure of the transferred plasmids proved identical with the original ones both in size and restriction pattern. Toxicity assays showed that the transformants gave a mortality of 90-100% against caterpillar of Heliothis assulta, indicating that the gene function was not changed by electroporation.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing.
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: P
authors.initials: P
authors.lastname: Liang
authors.forename: Y
authors.initials: Y
authors.lastname: Tian
authors.forename: K
authors.initials: K
authors.lastname: Mang
authors.forename: F
authors.initials: F
authors.lastname: Ba
authors.forename: P
authors.initials: P
authors.lastname: Ji
authors.forename: R
authors.initials: R
authors.lastname: Mei
mesh_terms: D001407:Bacillus / Q000235:genetics* / Q000254:growth & development; D001413:Bacillus thuringiensis / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D018274:Electroporation; D018014:Gene Transfer Techniques; D010957:Plasmids
chemical_list: D001427:Bacterial Toxins