title: Bacterial contamination of allografts.
abstract: The risk of bacterial infection through allogeneic bone transplantation is one of the problems facing tissue banks. The purpose of this study is to report the contamination rate in 987 grafts obtained under strictly aseptic conditions, between 1989 and 1992. The grafts were stored at -80 degrees C (cortical bone and tendons) and -40 degrees C (cancellous bone). The overall contamination rate was 6.6%, with Gram-positive bacteria responsible for 80% of the positive cultures. We discuss the sources of contamination, the most frequently isolated bacteria and the steps in the donation and transplantation procedures that help to reduce the risk of contamination. We conclude that the methods of procurement, processing and storage of tissues are effective in making sterile allografts available.
authors.affiliation: Department of Orthopedic Surgery and Traumatology, School of Medicine, University of Navarra, Pamplona, Spain.
authors.forename: R H
authors.initials: RH
authors.lastname: Barrios
authors.forename: M
authors.initials: M
authors.lastname: Leyes
authors.forename: S
authors.initials: S
authors.lastname: Amillo
authors.forename: C
authors.initials: C
authors.lastname: Oteiza
mesh_terms: D016025:Bone Transplantation; D001842:Bone and Bones / Q000382:microbiology*; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005615:Freezing; D006094:Gram-Positive Bacteria / Q000302:isolation & purification; D006801:Humans; D009343:Neisseria / Q000302:isolation & purification; D013710:Tendons / Q000382:microbiology* / Q000637:transplantation; D014021:Tissue Preservation; D014184:Transplantation, Homologous