title: Lymphocyte subpopulations in jejunal and ileal Peyer's patches of calves with experimental Salmonella dublin infection.
abstract: Changes of lymphocyte subpopulations in jejunal and ileal Peyer's patches (JPP and IPP) of six calves inoculated with Salmonella dublin were investigated at 9 hours, 1, 2, 3 and 7 days post inoculation (p.i.) using immunohistochemistry. Reactive areas and area percentages of B-lymphocytes, as well as CD4+, CD8+ and gamma delta T-lymphocytes within the different compartments of PP were estimated using computer-assisted morphometric analysis. A significant, linear decline of the areas of lymphoid follicles and domes in JPP and IPP due to depletion of B-lymphocytes was found. The rate of decline was similar in JPP and IPP, but more severe in lymphoid follicles than in domes. Intraepithelial cells in follicle-associated epithelium changed from predominantly B-lymphocytes in controls to CD8+ T-lymphocytes in inoculated calves and clusters of B-lymphocytes were observed above domes at days 1 and 2 p.i. Areas of CD4+ and CD8+ T-lymphocytes within lymphoid follicles and domes were increased at 3 and 7 days. p.i. resulting in decreased compartmentalization of the normally segregated T- and B-lymphocyte populations. The increase of CD4+ and CD8+ T-lymphocytes was, however, significant in lymphoid follicles in the JPP only. No significant changes in the amount and distribution of gamma delta T-lymphocytes were observed.
authors.affiliation: Department of Pathology, Tierärztliche Hochschule Hannover, Germany.
authors.forename: E M
authors.initials: EM
authors.lastname: Liebler
authors.forename: C M
authors.initials: CM
authors.lastname: Press
authors.forename: T
authors.initials: T
authors.lastname: Landsverk
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology*; D007082:Ileum / Q000473:pathology; D007583:Jejunum / Q000473:pathology; D016131:Lymphocyte Subsets; D008297:Male; D010581:Peyer's Patches / Q000473:pathology*; D012481:Salmonella Infections, Animal / Q000473:pathology*