Effects of tubeimoside-1 on HIV core protein p24 and cytopathogenesis in vitro.
Tubeimoside-1 (Tub) is a triterpenoid saponin first isolated in China from Bolbostemma paniculatum (Maxim) Franquet, Cucurbitaceae. To find out whether Tub has any anti-infective activity on human immunodeficiency virus (HIV), we tested its effects on HIV core protein p24 (with an ELISA) and HIV-mediated cytopathogenesis (using a colorimetric assay). The results showed that Tub inhibited both p24 production and cytopathogenesis mediated by HTLV-IIIB, and the median effective concentrations (EC50) were 24.1 and 22.9 micrograms.ml-1, respectively. Tub also effectively neutralized the infection of 2 other isolates, HTLV-IIIRF and HTLV-IIIMN. It is concluded that Tub had an inhibitory action on the infection of HIV-1 isolates and would be a promising candidate for treatment of AIDS.
Yu
L J
LJ
Department of Biochemistry, Shaanxi Provincial Academy of Traditional Chinese Medicine and Pharmacology, Xi-an, China.
Ma
R D
RD
Jiang
S B
SB
D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D016655:HIV Core Protein p24 / Q000096:biosynthesis*; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D016655:HIV Core Protein p24; D012503:Saponins; D014315:Triterpenes; C051084:tubeimoside I