Decreased bradykinin binding sites in fibroblasts from progressive systemic scleroderma.
The numbers of bradykinin receptors (BK-R) in cultured dermal fibroblasts from patients with progressive systemic sclerosis (PSS) and from healthy controls were measured using a receptor binding assay. The numbers of BK-R were significantly fewer in PSS fibroblasts than in control fibroblasts (P < 0.02). However, no differences in affinity were observed in BK-R between PSS and control fibroblasts. The BK-R mRNA levels were determined in PSS and control fibroblasts by Northern blot hybridization using BK-R cDNA, but no significant differences were found. These findings suggest that the decrease in BK-R in PSS fibroblasts might occur during a posttranslational step.
Ozeki
T
T
Department of Dermatology, Gifu University School of Medicine, Japan.
Osada
K
K
Seishima
M
M
Mori
S
S
Nozawa
Y
Y
D001665:Binding Sites; D002478:Cells, Cultured; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D018002:Receptors, Bradykinin / Q000032:analysis* / Q000235:genetics; D012595:Scleroderma, Systemic / Q000378:metabolism*
D012333:RNA, Messenger; D018002:Receptors, Bradykinin