A stable leukocyte-derived factor inhibits platelet aggregation.
The effect of human polymorphonuclear leukocyte (PMN)-derived supernatants on platelet aggregation was studied. The results showed that the supernatants produced a concentration-dependent inhibition of the platelet aggregation in response to thrombin. The effect was mainly on the second phase of the aggregation, suggesting that the secretory mechanisms of the platelets are disturbed. The activity of the supernatants was not reduced after treatment with heat (95 degrees C for 30 minutes), extreme pH (pH 12 or pH 2) or protease. In conclusion, human PMNs release a stable non-protein factor that inhibits platelet aggregation.
Liu
J J
JJ
University of Melbourne, Austin Hospital Department of Cardiac Surgery, Heidelberg, Vic., Australia.
Xie
B
B
Smith
I L
IL
Johnston
C I
CI
Buxton
B F
BF
D001685:Biological Factors / Q000097:blood* / Q000502:physiology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009504:Neutrophils / Q000502:physiology*; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D013917:Thrombin / Q000494:pharmacology; D014020:Tissue Extracts / Q000494:pharmacology
D001685:Biological Factors; D014020:Tissue Extracts; D013917:Thrombin