title: Recovery of heat-injured Listeria monocytogenes.
abstract: Concern has been expressed that the ability of heat-injured Listeria monocytogenes cells to resuscitate during refrigerated storage of food may lead to underestimation of their heat resistance. The recovery of heat-injured L. monocytogenes was therefore examined as a function of incubation temperature and composition of recovery medium. Heat-injured cells exhibited a broad optimum temperature for recovery centered around 20-25 degrees C. The best recovery medium of those tested was blood agar. Incubation of cells in broth or chicken slurry at 5 degrees C (cold-enrichment) did not allow repair of potentially lethal injury i.e. it did not allow recovery of cells that would otherwise have died if incubated at a higher temperature. In some cases incubation of heat-injured cells at 5 degrees C resulted in death of a proportion of the population. Repair of sublethal heat-injury, measured as the time of incubation in tryptone soya broth needed to regain the ability to grow on Listeria selective agar, was slower and less complete at 25 degrees C than at 2 degrees C; repair took 10-15 h at 25 degrees C compared with 8-12 days at 5 degrees C. Refrigeration of heat-treated foods should not therefore increase the risk that heat-injured cells will recover from the heat treatment.
authors.affiliation: BBSRC Institute of Food Research, Reading Laboratory, UK.
authors.forename: B M
authors.initials: BM
authors.lastname: Mackey
authors.forename: E
authors.initials: E
authors.lastname: Boogard
authors.forename: C M
authors.initials: CM
authors.lastname: Hayes
authors.forename: J
authors.initials: J
authors.lastname: Baranyi
mesh_terms: D015169:Colony Count, Microbial; D003470:Culture Media; D005516:Food Microbiology; D005519:Food Preservation; D006358:Hot Temperature; D008089:Listeria monocytogenes / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D012034:Refrigeration; D013696:Temperature
chemical_list: D003470:Culture Media