The purified SoxABCD quinol oxidase complex of Sulfolobus acidocaldarius contains a novel haem.
A respiratory quinol oxidase complex that is encoded by the soxABCD operon has been purified from the thermoacidophilic archaeon Sulfolobus acidocaldarius. The enzyme was solubilized with dodecyl maltoside and purified in the presence of this detergent and ethylene glycol. The complex is hydrodynamically homogeneous and contains at least five different polypeptides. In addition to the major subunits SoxA, SoxB and SoxC, it has two small polypeptides. One of these is the translation product of a short open reading frame (now called the soxD gene) at the end of the operon. The optical and electron paramagnetic resonance spectra of the SoxABCD complex have been characterized. It probably contains four A-type haems which are bound to SoxB and SoxC. The structure of these haems is not identical to haem A. The novel haem As has a 2-hydroxyethyl geranylgeranyl in position 2 of the porphyrin ring whereas haem A has the related farnesyl-containing side-chain.
Lübben
M
M
European Molecular Biology Laboratory, Heidelberg, Germany.
Warne
A
A
Albracht
S P
SP
Saraste
M
M
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003198:Computer Simulation; D003574:Cytochrome c Group / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004578:Electron Spin Resonance Spectroscopy; D008401:Gas Chromatography-Mass Spectrometry; D005798:Genes, Bacterial; D006418:Heme / Q000031:analogs & derivatives* / Q000302:isolation & purification; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification; D008958:Models, Molecular; D015394:Molecular Structure; D009876:Operon; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016944:Sulfolobus / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D008565:Membrane Proteins; C027728:heme a; D006418:Heme; D010088:Oxidoreductases; C090100:dibenzothiophene monooxygenase; C090099:diheme cytochrome c; C064500:flavocytochrome c sulfide dehydrogenase