title: Cloned cells develop renal cortical collecting tubules.
abstract: We investigated the development and morphogenesis of stem cells cloned from cultured transformed collecting duct cells of the rabbit in the presence of hepatocyte growth factor/scatter factor (HGF), vitamin A, laminin and transforming growth factors, HGF and vitamin A induced peanut agglutinin (PNA) positivity in nonprincipal cells (PC) but not in PCs, only HGF induced tubule formation. Although HGF induced a tubule-like arrangement in both cell lines, tubules with PNA-positive cells were observed only in non-PC cells incubated with HGF. Vasopressin increased intracellular free calcium in PNA-negative cells but not in PNA-positive cells. Intracellular pH increased in PNA-positive cells but not in PNA-negative cells when gluconate was substituted for Cl in the buffer. Cloned non-PCs were stem cells that developed PC- and IC-like characteristics and formed collecting ducts when incubated with HGF.
authors.affiliation: Department of Pharmacology, Jichi Medical School, Tochigi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Ikeda
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: O
authors.initials: O
authors.lastname: Sakai
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002999:Clone Cells; D000242:Cyclic AMP / Q000096:biosynthesis; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D007685:Kidney Tubules, Collecting / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D007797:Laminin / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology; D019887:Peanut Agglutinin; D011817:Rabbits; D012995:Solubility; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D015291:Transforming Growth Factors / Q000494:pharmacology; D014801:Vitamin A / Q000494:pharmacology
chemical_list: D007797:Laminin; D037102:Lectins; D019887:Peanut Agglutinin; D014801:Vitamin A; D017228:Hepatocyte Growth Factor; D015291:Transforming Growth Factors; D000242:Cyclic AMP