The polymerase chain reaction detects B cell clonalities in patients with Sjögren's syndrome and suspected malignant lymphoma.
OBJECTIVE
To investigate the usefulness of the polymerase chain reaction (PCR) to detect B cell clonal expansions in tissues where lymphoproliferative disease was suspected in the course of Sjögren's syndrome (SS).


METHODS
Tissue samples from 7 patients with definite (5 cases) or probable (2 cases) SS were subjected to routine histopathologic studies, immunophenotyping, and genotypic analysis, including Southern blotting and PCR amplification of immunoglobulin heavy chain (IgH) variable-diversity-joining (VDJ) region gene rearrangements.


RESULTS
Malignant lymphoma was detected in 3 cases, and myoepithelial sialadenitis in the remaining 4. B cell clonal expansion was detected in 7/7 cases by PCR, 5/7 by Southern blotting, and in no case of myoepithelial sialadenitis by kappa/lambda light chain immunophenotyping.


CONCLUSIONS
PCR may represent a quick and powerful tool to detect B cell clonalities in SS. Such information may provide new insights into the pathogenesis of the disease and may improve the clinical approach to the patients.
De Vita
S
S
Division of Experimental Oncology 1, Centro di Riferimento Oncologico, Aviano (PN), Italy.
Ferraccioli
G
G
De Re
V
V
Dolcetti
R
R
Carbone
A
A
Bartoli
E
E
Boiocchi
M
M
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D015139:Blotting, Southern; D005260:Female; D015322:Gene Rearrangement, B-Lymphocyte; D005803:Genes, Immunoglobulin; D005838:Genotype; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D016130:Immunophenotyping; D008228:Lymphoma, Non-Hodgkin / Q000150:complications / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012793:Sialadenitis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D012859:Sjogren's Syndrome / Q000150:complications / Q000235:genetics / Q000276:immunology* / Q000473:pathology
D007143:Immunoglobulin Heavy Chains