title: Dose-effect relationship for in vivo and in vitro induction of dicentric aberrations in blood lymphocytes of children.
abstract: Chromosome aberrations induced in vivo were studied in nine children 5-12 years old treated with total-body high-energy photon irradiation (pulsed exposure from a LINAC) for different types of malignant diseases. Dose-effect relationships were obtained for each child by taking blood at different times during exposure. In vitro dose-effect relationships for chromosome aberrations in children and adults were obtained by exposing blood under the same conditions as the children. Exposure in vivo and in vitro yielded similar linear-quadratic dose-effect relationships for dicentric aberrations. The response in vitro was slightly greater than in vivo, but the difference was not very large. It is concluded that the dose-effect relationship for dicentric chromosome aberrations obtained in vitro for adults can be used for biological dosimetry in irradiated children. Some of the children displayed a high number of "rogue cells" before exposure; this may be due to the malignant disease as it was not found in the healthy controls.
authors.affiliation: Teratogenicity and Mutagenicity Unit, Catholic University of Louvain, Brussels, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Léonard
authors.forename: I
authors.initials: I
authors.lastname: Baltus
authors.forename: E D
authors.initials: ED
authors.lastname: Léonard
authors.forename: G B
authors.initials: GB
authors.lastname: Gerber
authors.forename: F
authors.initials: F
authors.lastname: Richard
authors.forename: A
authors.initials: A
authors.lastname: Wambersie
mesh_terms: D000293:Adolescent; D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D003131:Combined Modality Therapy; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000532:radiotherapy; D008214:Lymphocytes / Q000528:radiation effects*; D008223:Lymphoma / Q000188:drug therapy / Q000532:radiotherapy; D008297:Male; D009447:Neuroblastoma / Q000188:drug therapy / Q000532:radiotherapy; D010315:Particle Accelerators; D014916:Whole-Body Irradiation