title: Rapid differential diagnosis of rinderpest and peste des petits ruminants using an immunocapture ELISA.
abstract: An immunocapture ELISA for the diagnosis of rinderpest and peste des petits ruminants is described. Monoclonal antibodies directed against non-overlapping antigenic domains on the nucleocapsid (N) were used to detect the virus N protein in supernatants from infected cells and in field specimens. The assay, which is very sensitive, can be performed in one hour on pre-coated plates. There was no cross reaction between the two viruses in the test and the N protein could be detected in infected cell supernatants kept at ambient temperature for one week. These results show that the ELISA is suitable for routine diagnosis of field samples.
authors.forename: G
authors.initials: G
authors.lastname: Libeau
authors.forename: A
authors.initials: A
authors.lastname: Diallo
authors.forename: F
authors.initials: F
authors.lastname: Colas
authors.forename: L
authors.initials: L
authors.lastname: Guerre
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002478:Cells, Cultured; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D015511:Goat Diseases / Q000175:diagnosis*; D006041:Goats; D018185:Morbillivirus Infections / Q000175:diagnosis / Q000662:veterinary*; D018107:Peste-des-petits-ruminants virus; D012301:Rinderpest / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D014709:Vero Cells
chemical_list: D000914:Antibodies, Viral