A survey of Campylobacter jejuni in broilers from assignment to slaughter using DNA-DNA hybridization.
A survey of Campylobacter jejuni in the cecal contents of broilers raised on a farm was carried out by the DNA-DNA hybridization method from the day of assignment to slaughter at about 1-week intervals. C. jejuni was detected in chickens as early as 1 week of age, and was widely detected at each week of age throughout the growing period. In addition, of 20 chickens tested just after assignment, 7 (35%) were C. jejuni positive. It is suggested that newly introduced chickens may have already been contaminated with C. jejuni. The hybridization method was able to detect C. jejuni in the chickens from the day of assignment to 3 weeks of age, but C. jejuni was never detected in the same chickens by the enriched culture method. Therefore, it is suggested that the hybridization method is more sensitive than the enriched culture method.
Chuma
T
T
Department of Veterinary Public Health, Faculty of Agriculture, Kagoshima University, Japan.
Yamada
T
T
Yano
K
K
Okamoto
K
K
Yugi
H
H
D000367:Age Factors; D000822:Animal Husbandry; D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification*; D002432:Cecum / Q000382:microbiology; D002645:Chickens / Q000254:growth & development / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D007564:Japan; D009693:Nucleic Acid Hybridization
D004269:DNA, Bacterial