Sensitive method for measuring lactate dehydrogenase activity in human serum by differential-pulse polarography.
A sensitive and accurate differential-pulse polarographic method is reported for the measurement of lactate dehydrogenase (LDH) activity. The method is based on the polarographic measurement of the NAD+ formed via the enzymic reaction of LDH with pyruvate and NADH. The method was successfully applied to measure LDH activity in the serum of normal and diseased individuals.
Sulaiman
S T
ST
Department of Chemistry, College of Science, University of Mosul, Iraq.
al-Najafi
T S
TS
Hamdon
H S
HS
D004194:Disease; D006801:Humans; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D009243:NAD / Q000032:analysis; D011048:Polarography / Q000379:methods; D011773:Pyruvates; D012016:Reference Values; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents; D011773:Pyruvates; D009243:NAD; D007770:L-Lactate Dehydrogenase