[Experimental study on the role of oxygen-free radicals in ischemic reperfusion tissue injury is island skin flaps].
In this study the role of oxygen-free radicals and the effect of treatment with superoxide dismutase (SOD) had been investigated in a 3 x 6 cm2 abdominal area island skin flaps of rat model which were subjected to 8 hours of total venous occlusion. In experiment 1, 56 rats were divided into seven test groups. Tissue assay for Malonydialdehyde (MDA) were obtained. Flaps with ischemia had higher MDA at 8.12 hours and all reperfusion groups had higher MDA as compared to the control groups (P < 0.01). In reperfusion groups, the MDA had increased at 4.8 hours compared to ischemia group at the same time. In experimental 2, 28 rats were used. Treatment with superoxide dismutase injected into the artery following the onset of reperfusion significantly enhanced island flap survival from 28.6% (4/14) to 78.6% (11/14) (P < 0.01).
Hu
Y
Y
Beijing Stomatology Hospital.
D000818:Animals; D016166:Free Radical Scavengers; D005609:Free Radicals; D008315:Malondialdehyde / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology; D013482:Superoxide Dismutase / Q000627:therapeutic use*; D013524:Surgical Flaps
D016166:Free Radical Scavengers; D005609:Free Radicals; D008315:Malondialdehyde; D013482:Superoxide Dismutase