title: Factors affecting the transformation of octacalcium phosphate to apatite in vitro.
abstract: A fresh octacalcium phosphate (OCP) precipitate without drying and three kinds of dried OCP powders were soaked for 3 weeks in 11 kinds of physiological solutions consisting of different combinations of Ca2+, Mg2+, K+, Na+, HCO3-, HPO4(2-), F-, albumin, collagen and alkaline phosphatase, in three different pH values and at three different temperatures. X-ray diffraction study showed that most of the OCP had been transformed to apatite with low crystallinity after soaking in the solutions without Mg2+. The IR absorption spectra revealed that CO3 was incorporated in the apatite formed from OCP in the solution without Mg2+, whereas OCP changed little after soaking in the solution containing Mg2+. These results suggest that the presence of Mg2+ in the solution is one of the most effective means of inhibiting the transformation of OCP to apatite by interrupting the precipitation process of apatite.
authors.affiliation: Department of Dental Material Science, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ban
authors.forename: M
authors.initials: M
authors.lastname: Matsuura
authors.forename: N
authors.initials: N
authors.lastname: Arimoto
authors.forename: J
authors.initials: J
authors.lastname: Hayashizaki
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: J
authors.initials: J
authors.lastname: Hasegawa
mesh_terms: D000704:Analysis of Variance; D001031:Apatites / Q000138:chemical synthesis*; D002130:Calcium Phosphates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D002130:Calcium Phosphates; C022045:octacalcium phosphate; D008274:Magnesium