title: Differential expression of two major cytokines produced by neutrophils, interleukin-8 and the interleukin-1 receptor antagonist, in neutrophils isolated from the synovial fluid and peripheral blood of patients with rheumatoid arthritis.
abstract: OBJECTIVE
  Neutrophils have been shown to produce interleukin-8 (IL-8) and interleukin-1 receptor antagonist (IL-1ra) in large amounts compared with other cytokines. Since IL-8 has a proinflammatory action whereas IL-1ra is antiinflammatory, our objective was to examine the relative levels of production of these cytokines by synovial fluid (SF) neutrophils isolated from patients with rheumatoid arthritis (RA).
  
  
  METHODS
  We measured cytokine production using an enzyme-linked immunosorbent assay and analyzed messenger RNA accumulation in cells by Northern blot.
  
  
  RESULTS
  SF neutrophils produced significantly more IL-8 and IL-1 beta, but significantly less IL-1ra, than peripheral blood neutrophils.
  
  
  CONCLUSIONS
  These observations provide new information on the production of pro- and antiinflammatory molecules by neutrophils in the SF environment, and their possible role in RA.
authors.affiliation: Université Laval Sante-Foy, Quebec, Canada.
authors.forename: A D
authors.initials: AD
authors.lastname: Beaulieu
authors.forename: S R
authors.initials: SR
authors.lastname: McColl
mesh_terms: D001172:Arthritis, Rheumatoid / Q000097:blood / Q000473:pathology; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis* / Q000235:genetics; D009504:Neutrophils / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis* / Q000235:genetics; D013582:Synovial Fluid / Q000166:cytology
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D016209:Interleukin-8; D012333:RNA, Messenger; D012795:Sialoglycoproteins