Alignment of physical and genetic maps of human 8q23-qter using somatic cell hybrid mapping panel.
We describe a mapping panel for the 8q23-qter region composed of human-hamster hybrid cell lines carrying deletion and translocation derivatives of human chromosome 8. The panel divides this region of the chromosome into nine intervals and has been used to map 40 loci by Southern blot hybridization and PCR. Use of this mapping panel has allowed us to align the terminal portions of two different genetic maps of chromosome 8 with each other and with the physical map of the chromosome.
Parrish
J E
JE
Department of Biology, University of Houston, Texas 77204-5513.
Wang
Y
Y
Wagner
M J
MJ
Wells
D E
DE
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D006224:Cricetinae; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D016133:Polymerase Chain Reaction
D015342:DNA Probes