Non-systemic expression of a stress-responsive maize polyubiquitin gene (Ubi-1) in transgenic rice plants.
We have used the promoter, 1st exon and 1st intron of the maize polyubiquitin gene (Ubi-1) for rice transformation experiments and revealed the characteristic expression of Ubi-1 gene: (1) Ubi-1 gene is not regulated systemically but rather individual cells respond independently to the heat or physical stress; (2) Ubi-1 gene changes its tissue-specific expression in response to stress treatment; (3) the expression of Ubi-1 gene is dependent on cell cycle.
Takimoto
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Department of Biological Science, Faculty of Science, Hokkaido University, Sapporo, Japan.
Christensen
A H
AH
Quail
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PH
Uchimiya
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Toki
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D000123:Acetyltransferases / Q000096:biosynthesis / Q000235:genetics; D000222:Adaptation, Physiological / Q000235:genetics; D001704:Biopolymers / Q000096:biosynthesis / Q000235:genetics*; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D006540:Herbicides / Q000494:pharmacology; D006358:Hot Temperature; D007438:Introns / Q000235:genetics; D009943:Organophosphorus Compounds / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018520:Plant Shoots / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects; D025821:Polyubiquitin; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014018:Tissue Distribution; D014452:Ubiquitins / Q000096:biosynthesis / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D001704:Biopolymers; D006540:Herbicides; D009943:Organophosphorus Compounds; D011993:Recombinant Fusion Proteins; D014452:Ubiquitins; D025821:Polyubiquitin; C003122:bialaphos; D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase; D005966:Glucuronidase