In vivo observation of the human tear film by tandem scanning confocal microscopy.
A new method for observing normal and pathologic states of the human tear film using tandem scanning confocal microscopy is presented. The confocal microscope is configured with a horizontal light path, a 10 x dry objective, and an image-intensified camera for collecting images at a magnification of approximately 150x. The advantages of confocal microscopy can be used to collect reflected images of the human tear film with improved detail and resolution.
Mathers
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WD
Department of Ophthalmology, University of Iowa Hospitals and Clinics, Iowa City 52242-1091.
Daley
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TE
D006801:Humans; D008055:Lipids / Q000032:analysis; D018613:Microscopy, Confocal / Q000379:methods; D013666:Tears / Q000737:chemistry / Q000378:metabolism*
D008055:Lipids