title: Crystallization and preliminary X-ray diffraction studies on ADP-ribosylation factor 1.
abstract: ADP-ribosylation factor 1 (ARF-1) is a member of a family of small G-proteins that regulate both intracellular vesicle transport and phospholipase D activity. Crystals of ARF-1 suitable for X-ray diffraction analysis have been grown in the presence of GDP by the hanging drop vapour diffusion method. Crystals grow in space group C2 with cell dimensions a = 122.36 A, b = 45.01 A, c = 91.96 A and beta = 133.62 degrees and diffract to at least 2.3 A resolution. A second crystal form has been characterized (space group C2, a = 69.70 A, b = 45.25 A, c = 60.45 A, beta = 109.6 degrees) but does not grow reproducibly.
authors.affiliation: Department of Biomolecular Structure, Glaxo Research and Development, Greenford, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Greasley
authors.forename: H
authors.initials: H
authors.lastname: Jhoti
authors.forename: A C
authors.initials: AC
authors.lastname: Fensome
authors.forename: S
authors.initials: S
authors.lastname: Cockcroft
authors.forename: G M
authors.initials: GM
authors.lastname: Thomas
authors.forename: B
authors.initials: B
authors.lastname: Bax
mesh_terms: D020727:ADP-Ribosylation Factors; D002352:Carrier Proteins / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D019204:GTP-Binding Proteins / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors