title: Expression of mosquitocidal toxin genes in a gas-vacuolated strain of Ancylobacter aquaticus.
abstract: A series of plasmids bearing the binary toxin genes of Bacillus sphaericus 2297 or 2317.3, the 100-kDa toxin gene of B. sphaericus SSII-1, or the 130-kDa (cryIVB) toxin gene of Bacillus thuringiensis subsp. israelensis were constructed and introduced into Ancylobacter aquaticus by electroporation. The transformed A. aquaticus cells exhibited significant toxicity towards mosquito larvae, demonstrating a potential use of recombinant A. aquaticus for biological control of mosquitoes.
authors.affiliation: Institute of Molecular and Cell Biology, National University of Singapore.
authors.forename: W H
authors.initials: WH
authors.lastname: Yap
authors.forename: T
authors.initials: T
authors.lastname: Thanabalu
authors.forename: A G
authors.initials: AG
authors.lastname: Porter
mesh_terms: D000818:Animals; D001407:Bacillus / Q000235:genetics*; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics* / Q000633:toxicity; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics*; D007306:Insecticides; D007814:Larva / Q000187:drug effects; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D014169:Transformation, Bacterial; D014871:Water Microbiology
chemical_list: D001427:Bacterial Toxins; D007306:Insecticides