Molecular cloning and functional expression of the guinea pig cardiac Na(+)-Ca2+ exchanger.
The cDNA of the guinea pig cardiac Na(+)-Ca2+ exchanger was cloned from a lambda ZAP cDNA library. The deduced sequence of the protein corresponds to 970 amino acids and is 98% identical to the canine cardiac exchanger. The leader peptide region shows substantial variation among species. The cloned cDNA can induce Na(+)-Ca2+ exchange activity when in vitro transcribed cRNA is injected into Xenopus laevis oocytes.
Tsuruya
Y
Y
Department of Physiology, UCLA School of Medicine 90024-1760.
Bersohn
M M
MM
Li
Z
Z
Nicoll
D A
DA
Philipson
K D
KD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D019831:Sodium-Calcium Exchanger
D002352:Carrier Proteins; D018076:DNA, Complementary; D019831:Sodium-Calcium Exchanger; D012964:Sodium; D002118:Calcium