title: Fast protein sequence verification by matrix assisted laser desorption mass spectrometric analysis of whole enzymatic digests.
abstract: Matrix assisted laser desorption mass spectrometry provides a very fast and efficient way to check protein sequences by the analysis of whole proteolytic digests. This method has been applied to the sequence verification of E. coli isoleucyl tRNA synthetase, for which two different sequences are found in data banks. The result of this investigation clearly shows that no one of these sequences is correct.
authors.affiliation: Laboratoire de Biochimie, CNRS URA 240, Ecole Polytechnique, Palaiseau, France.
authors.forename: B M
authors.initials: BM
authors.lastname: Hoang
authors.forename: P
authors.initials: P
authors.lastname: Dessen
authors.forename: J M
authors.initials: JM
authors.lastname: Schmitter
mesh_terms: D000595:Amino Acid Sequence; D010450:Endopeptidases; D007533:Isoleucine-tRNA Ligase / Q000737:chemistry / Q000378:metabolism; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D008666:Metalloendopeptidases; D008718:Methionine-tRNA Ligase / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis / Q000379:methods*; D012697:Serine Endopeptidases; D014357:Trypsin
chemical_list: D010450:Endopeptidases; D012697:Serine Endopeptidases; D014357:Trypsin; D008666:Metalloendopeptidases; C058921:endoproteinase Asp-N; D008718:Methionine-tRNA Ligase; D007533:Isoleucine-tRNA Ligase