title: Isolation of mouse complement component C7.
abstract: Mouse complement component C7 was purified from serum by a sequential procedure of fractionation precipitation by ammonium sulfate, followed by DE-52 anion exchange chromatography. Protein G affinity column chromatography, Mono S cation exchange chromatography and Superdex 200 gel filtration. The final product contained a highly purified mouse C7 component showing a single band on SDS-PAGE at the apparent Mrs of 90 kDa and 100 kDa under non-reduced and reduced conditions respectively. The yield of C7, which was measured by the biological activity, was 7.0%
authors.affiliation: Department of Clinical Laboratory Medicine, Ehime University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hitsumoto
authors.forename: H
authors.initials: H
authors.lastname: Ohnishi
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Nishimukai
authors.forename: S
authors.initials: S
authors.lastname: Saheki
authors.forename: N
authors.initials: N
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D005591:Chemical Fractionation; D002845:Chromatography / Q000379:methods; D003184:Complement C7 / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008811:Mice, Inbred DBA
chemical_list: D003184:Complement C7