Effect of gamma carboxylation on prothrombinase inhibitory activity of catalytically inactive factor XA.
Recombinant catalytically inactive factor Xa (factor rXai) is capable of assembly into inactive prothrombinase complexes, thus serving as a competitive inhibitor (Ki = 0.3nM) of active factor Xa. In order to study the role of gamma carboxylation in prothrombinase complex assembly, we have prepared differentially gamma carboxylated factor rXai and have measured the activities of these proteins in prothrombinase complex inhibition and in extension of plasma clotting. A factor rXai preparation containing 8 out of a possible maximum of 11 g carboxyglutamic acid (GLA) residues was found to be as active as chemically inactivated plasma factor Xa which was fully gamma carboxylated. Loss of a single additional g carboxyglutamic acid in the recombinant protein, however lead to a marked loss in activity. Factor rXai preparation with 8 GLA residues is also detected by a monoclonal antibody specific for a GLA dependent epitope. Thus assembly of the factor Va/Xa complex on phospholipid membranes does not require the presence of all of the g carboxyglutamic acid residues present in the plasma protein.
Sinha
U
U
COR Therapeutics Inc., South San Francisco, CA 94080.
Hancock
T E
TE
Nzerem
J J
JJ
Lin
P H
PH
Tomlinson
J E
JE
Wolf
D L
DL
D015055:1-Carboxyglutamic Acid / Q000032:analysis; D000818:Animals; D000918:Antibody Specificity; D016466:CHO Cells; D002264:Carboxylic Acids / Q000378:metabolism*; D002384:Catalysis; D002478:Cells, Cultured; D006224:Cricetinae; D015951:Factor Xa / Q000096:biosynthesis*; D065427:Factor Xa Inhibitors; D010314:Partial Thromboplastin Time; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D013925:Thromboplastin / Q000037:antagonists & inhibitors / Q000378:metabolism*
D002264:Carboxylic Acids; D065427:Factor Xa Inhibitors; D011994:Recombinant Proteins; C088216:factor rXai; D015055:1-Carboxyglutamic Acid; D013925:Thromboplastin; D015951:Factor Xa