Altered cytosolic calcium homeostasis in rat cardiac myocytes in CRF.
Chronic renal failure (CRF) is associated with an increase in calcium content of heart. This was attributed to the secondary hyperparathyroidism of CRF, since PTH augments entry of calcium into cardiac myocytes. At present, it is not known whether the increase in calcium content of heart reflects a rise in basal levels of cytosolic calcium ([Ca2+]) of cardiac myocytes. Further, in order for the PTH-induced entry of calcium into cardiac myocytes to raise their basal levels of [Ca2+]i, calcium extrusion out of these cells should be impaired as well. The present study examined the effect of CRF with and without excess PTH (PTX) and of the treatment of CRF rats with verapamil on basal levels of [Ca2+]i and ATP content of cardiac myocytes and on the activities of the pumps that are directly (Ca(2+)-ATPase and Na(+)-Ca2+ exchanger) and indirectly (Na(+)-K+ ATPase) responsible for calcium extrusion out of these cells. The basal levels of [Ca2+]i of cardiac myocytes increased (P < 0.01) and their ATP content decreased (P < 0.01) as the duration of CRF advanced. CRF was associated with significant decrement in Vmax of Ca2+ ATPase and Na(+)-K+ ATPase and in Na(+)-Ca2+ exchange. These derangements were prevented by prior PTX of the CRF rats or by their treatment with verapamil.(ABSTRACT TRUNCATED AT 250 WORDS)
Zhang
Y B
YB
Division of Nephrology, University of Southern California, School of Medicine, Los Angeles.
Smogorzewski
M
M
Ni
Z
Z
Massry
S G
SG
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D006706:Homeostasis; D006962:Hyperparathyroidism, Secondary / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019831:Sodium-Calcium Exchanger; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014700:Verapamil / Q000008:administration & dosage
D002352:Carrier Proteins; D010281:Parathyroid Hormone; D019831:Sodium-Calcium Exchanger; D000255:Adenosine Triphosphate; D014700:Verapamil; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium