Gene regulation during neuronal and non-neuronal differentiation of NTERA2 human teratocarcinoma-derived stem cells.
We constructed cDNA libraries from a clonal human teratocarcinoma-derived cell line and two retinoic acid-induced derivatives: a homogeneous population of neurons and a FACS-isolated, non-neuronal population. These libraries are large and representative of the cells from which they were derived, as determined by colony hybridization. PCR analysis indicates that the transcripts encoding P- and E-cadherin are down-regulated whereas the the prion protein (PrP) transcript is up-regulated in neurons. These cells offer a promising system for investigations of human prion infection and the cDNA libraries provide a source of neuron-specific genes.
Ackerman
S L
SL
Jackson Laboratory, Bar Harbor, ME 04609.
Knowles
B B
BB
Andrews
P W
PW
D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002469:Cell Separation; D054278:Embryonal Carcinoma Stem Cells; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000166:cytology*; D018243:Teratocarcinoma / Q000235:genetics* / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D014212:Tretinoin