Hippocampal long-term potentiation in vivo induces translocation of protein kinase C gamma.
The possible involvement of the Ca(2+)-dependent protein kinase C (PKC) isoenzymes alpha/beta and gamma in mechanisms of long-term potentiation (LTP) was investigated after tetanic stimulation of the perforant path in vivo. Brief tetanic stimulation of the perforant path resulted in a 150% increase in population spike amplitude recorded from the dentate gyrus synapses in response to test stimuli 5 and 10 min after tetanization. Immunoblot analysis of PKC immunoreactivity in cytosolic and membrane fractions revealed a LTP-induced translocation of gamma PKC but not alpha/beta PKC into the cytosol in dentate gyrus but also in the other ipsilateral hippocampal regions. These data suggest different physiological roles of Ca(2+)-dependent PKC isoenzymes in activity-dependent synaptic plasticity.
Angenstein
F
F
Institute for Neurobiology, Laboratory for Cellular Signalling, Magdeburg, Germany.
Riedel
G
G
Reymann
K G
KG
Staak
S
S
D000818:Animals; D003600:Cytosol / Q000201:enzymology*; D004558:Electric Stimulation; D006624:Hippocampus / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism*; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D007527:Isoenzymes; C087857:protein kinase C gamma; D011493:Protein Kinase C