In vivo 31P NMR studies of orientation effects upon rat brain metabolism during mild hypoxia.
Energy metabolites in rat brain under the same level of hypoxia were monitored by 31P NMR in both horizontal and vertical magnets. The changes in PCr, Pi, and pHi in the vertical setting toward the end of hypoxia were significantly larger and the recovery in the horizontally held animals was more complete. The results demonstrated quantitatively that the stress of the alignment is superimposed on the stress of hypoxia in the vertical magnet.
Chen
M
M
Department of Medicinal Chemistry, University of Utah, Salt Lake City 84112.
Stolk
J A
JA
Olsen
J I
JI
Schweizer
M P
MP
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002245:Carbon Dioxide / Q000097:blood; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D002534:Hypoxia, Brain / Q000097:blood / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D008297:Male; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption / Q000502:physiology; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D010759:Phosphorus Isotopes; D011187:Posture / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors
D010710:Phosphates; D010759:Phosphorus Isotopes; D010725:Phosphocreatine; D002245:Carbon Dioxide; D000255:Adenosine Triphosphate; D010100:Oxygen