title: Microencapsulation of Lactococcus lactis subsp. cremoris.
abstract: Lactococcus lactis subsp. cremoris was microencapsulated within alginate/poly-L-lysine (alg/PLL), nylon or crosslinked polyethyleneimine (PEI) membranes. Toxic effects were observed with solvents and reagents used in nylon and PEI membrane formation. Alg/PLL encapsulation resulted in viable and active cell preparations which acidified milk at a rate proportional to the cell concentration, but at rates less than that of free cell preparations. At 4 x 10(8) colony-forming units (cfu/ml milk), encapsulated cells took 17 per cent longer than free lactococci to reduce the pH of milk to 5.5. Similar activities of free and micro-encapsulated cells may be attained at higher cell concentrations (10(9) cfu/ml milk). The rate of lactic acid production was approximately 2 mmol/h at an encapsulated cell concentration of 4 x 10(8) cfu/ml.
authors.affiliation: Department of Chemical Engineering, McGill University, Montréal, Québec, Canada.
authors.forename: B C
authors.initials: BC
authors.lastname: Larisch
authors.forename: D
authors.initials: D
authors.lastname: Poncelet
authors.forename: C P
authors.initials: CP
authors.lastname: Champagne
authors.forename: R J
authors.initials: RJ
authors.lastname: Neufeld
mesh_terms: D000464:Alginates; D002470:Cell Survival; D002478:Cells, Cultured; D004339:Drug Compounding; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D013294:Lactococcus lactis / Q000166:cytology* / Q000378:metabolism; D008567:Membranes, Artificial; D009757:Nylons; D011094:Polyethyleneimine; D011107:Polylysine; D012997:Solvents
chemical_list: D000464:Alginates; D007773:Lactates; D008567:Membranes, Artificial; D009757:Nylons; D012997:Solvents; D011107:Polylysine; D019344:Lactic Acid; D011094:Polyethyleneimine