title: Male meiotic segregation of gonosomes analysed by two colour FISH in human interphase spermatozoa.
abstract: Human meiotic segregation of X and Y chromosomes was simultaneously analysed by dual fluorescence in situ hybridization (FISH) on 10,638 interphase spermatozoa from the same donor. A modified method for sperm decondensation ensured access of both X and Y probes to the sperm chromatin and a 99% hybridization efficiency. Expected sex ratios were obtained (49.30% haploidy X and 49.22% haploidy Y). The frequencies of meiotic II non-disjunctions for X and Y chromosomes (0.05%) were similar to those observed in sperm karyotypes after heterospecific fertilization of hamster eggs. In contrast, the frequency of XY bearing cells was significantly higher (0.42%). However, XY cells detected by FISH could either be diploid somatic cells, diploid germinal cells or hyperhaploid XY spermatozoa, the latter resulting from meiotic I non-disjunctions.
authors.affiliation: Cytogenetic and Reproductive Biology Laboratory, Grenoble University Medical School, France.
authors.forename: E
authors.initials: E
authors.lastname: Chevret
authors.forename: S
authors.initials: S
authors.lastname: Rousseaux
authors.forename: M
authors.initials: M
authors.lastname: Monteil
authors.forename: J
authors.initials: J
authors.lastname: Cozzi
authors.forename: R
authors.initials: R
authors.lastname: Pelletier
authors.forename: J
authors.initials: J
authors.lastname: Mollard
authors.forename: B
authors.initials: B
authors.lastname: Sèle
mesh_terms: D002869:Chromosome Aberrations; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007399:Interphase / Q000235:genetics; D008297:Male; D008540:Meiosis / Q000235:genetics*; D012730:Sex Chromosomes / Q000235:genetics*; D013094:Spermatozoa / Q000737:chemistry*; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D015342:DNA Probes