The composition and function of the pulmonary surfactant system during metamorphosis in the tiger salamander Ambystoma tigrinum.
Mammalian lungs secrete a mixture of surface-active lipids (surfactant), which greatly reduces the surface tension of the fluid coating the inner lung surface, thereby reducing the risk of collapse upon deflation and increasing compliance upon inflation. During foetal lung maturation, these lipids become enriched in the primary and active ingredient, a disaturated phospholipid. However, disaturated phospholipids exist in their inactive gel-like form at temperatures below 37 degrees C and thus are inappropriate for controlling surface tension in the lungs of many ectotherms. We examined the development of the composition and function of the surfactant system of the tiger salamander (Ambystoma tigrinum) during metamorphosis from the fully aquatic larva (termed stage I) through an intermediate air-breathing larval form (stage IV) to the terrestrial adult (stage VII). Biochemical analysis of lung washings from these three life stages revealed a decrease in the percentage of disaturated phospholipid per total phospholipid (23.03 versus 15.92%) with lung maturity. The relative cholesterol content remained constant. The increased level of phospholipid saturation in the fully aquatic larvae may reflect their generally higher body temperature and the higher external hydrostatic compression forces exerted on the lungs, compared to the terrestrial adults. Opening pressure (pressure required for initial lung opening) prior to lavage decreased from larval to adult salamanders (7.96 versus 4.69 cm H2O), indicating a decrease in resistance to opening with lung development.(ABSTRACT TRUNCATED AT 250 WORDS)
Orgeig
S
S
Department of Human Physiology, School of Medicine, Flinders University of South Australia, Adelaide.
Daniels
C B
CB
Smits
A W
AW
D000557:Ambystoma / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology*; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D008168:Lung / Q000254:growth & development / Q000502:physiology; D008675:Metamorphosis, Biological; D010743:Phospholipids / Q000378:metabolism; D011312:Pressure; D011663:Pulmonary Surfactants / Q000378:metabolism* / Q000502:physiology*
D010743:Phospholipids; D011663:Pulmonary Surfactants; D002784:Cholesterol