Lipidheme-microsphere (LH-M). A new type of totally synthetic oxygen carrier and its oxygen carrying ability.
We have succeeded in synthesizing a new type of totally artificial oxygen carrier which was produced by covering oil droplets (microsphere) with synthetic hemes (LH-M). We studied its oxygen-transporting ability in hemorrhagic dogs. Four beagles weighing about 8 kg were studied. Under controlled ventilation, exchange-transfusion of 30 ml/kg was carried out. Cardiac output, hemoglobin and LH-M concentration in the blood, and blood gas were measured to 5 hours after intravenous injection of LH-M solution. LH-M delivered 15.7 to 22.3 ml/min (11 to 16%) of oxygen to the tissue and 5.5 to 8.2 ml/min (11 to 17%) of oxygen was consumed from LH-M to 5 hours after intravenous injection. Its half-life time in the blood stream was about 12 hours. It was confirmed that LH-M transported oxygen and released it to the tissue in vivo.
Kakizaki
T
T
Department of Surgery, School of Medicine, Keio University, Japan.
Kobayashi
K
K
Komatsu
T
T
Nishide
H
H
Tsuchida
E
E
D000818:Animals; D001802:Blood Substitutes / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism*; D002302:Cardiac Output; D004285:Dogs; D005069:Evaluation Studies as Topic; D006418:Heme / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D006470:Hemorrhage / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy; D008055:Lipids; D008081:Liposomes; D008863:Microspheres; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D013997:Time Factors
D001802:Blood Substitutes; D006454:Hemoglobins; D008055:Lipids; D008081:Liposomes; D006418:Heme; D010100:Oxygen