title: [A model prokaryotic promotor. III. Cloning and study of functional activity in vivo].
abstract: The efficiency of two schemes of oligonucleotide-directed insertion mutagenesis was studied in comparison with standard cloning of DNA duplexes based on blunt-end ligation. Using new approaches, the 30-bp consensus-like prokaryotic promoter was inserted in proper orientation in a promoter-testing plasmid at an almost 100% frequency. Data on the marker gal operon expression and S1-nuclease mapping of the transcription initiation points indicate formation of an active promoter in the region of the insertion.
authors.forename: O N
authors.initials: ON
authors.lastname: Koroleva
authors.forename: I A
authors.initials: IA
authors.lastname: Shilov
authors.forename: V N
authors.initials: VN
authors.lastname: Sergeev
authors.forename: V L
authors.initials: VL
authors.lastname: Drutsa
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids; D011387:Prokaryotic Cells; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D004247:DNA
vernacular_title: Model'nyĭ prokarioticheskiĭ promotor. III. Klonirovanie i issledovanie funktsional'noĭ aktivnosti in vivo.