title: Growth inhibition of Trypanosoma cruzi and Leishmania donovani infantum by different snake venoms: preliminary identification of proteins from Cerastes cerastes venom which interact with the parasites.
abstract: Venom from three different snake species was tested in vitro against the protozoan parasites Trypanosoma cruzi and Leishmania donovani infantum. Two of them, Cerastes cerastes and Naja haje, exerted a significant growth inhibition of T. cruzi and L. d. infantum parasites. Heating of the venoms abolished their activity, suggesting that the active factors are thermolabile. Incubation of parasites with 125I-labelled C. cerastes venom proteins allowed preliminary identification of components which interact preferentially with the pathogens.
authors.affiliation: Laboratoire de Recherche sur les Trypanosomatidae, INSERM U415, Institut Pasteur de Lille, France.
authors.forename: R
authors.initials: R
authors.lastname: Fernandez-Gomez
authors.forename: H
authors.initials: H
authors.lastname: Zerrouk
authors.forename: F
authors.initials: F
authors.lastname: Sebti
authors.forename: M
authors.initials: M
authors.lastname: Loyens
authors.forename: A
authors.initials: A
authors.lastname: Benslimane
authors.forename: M A
authors.initials: MA
authors.lastname: Ouaissi
mesh_terms: D000818:Animals; D001345:Autoradiography; D003470:Culture Media; D004546:Elapid Venoms / Q000737:chemistry / Q000494:pharmacology*; D017815:Elapidae; D004591:Electrophoresis, Polyacrylamide Gel; D007893:Leishmania donovani / Q000187:drug effects* / Q000254:growth & development; D011506:Proteins / Q000032:analysis*; D013696:Temperature; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development; D014757:Viper Venoms / Q000737:chemistry / Q000494:pharmacology*; D017819:Viperidae
chemical_list: D003470:Culture Media; D004546:Elapid Venoms; D011506:Proteins; D014757:Viper Venoms