title: Germinating conidiospores of Aspergillus amino acid auxotrophs are hypersensitive to heat shock, oxidative stress and DNA damage.
abstract: Germinating conidiospores (conidia) of Aspergillus nidulans amino acid-requiring strains are hypersensitive to heat, oxidative stress, UV radiation and chemical mutagens when compared with other strains. They also showed an increased mutation rate. Sensitivity to stress conditions has been correlated with an abnormal RAS/cAMP pathway in mutants of S. cerevisiae. We suggest that the RAS/cAMP pathway is defective in germinating conidia of Aspergillus amino acid auxotrophs and that this is responsible for suppressing DNA repair and conferring sensitivity to oxidative stress and heat shock.
authors.affiliation: Department of Biological and Biomedical Sciences, University of Ulster, Jordanstown, N. Ireland, UK.
authors.forename: E
authors.initials: E
authors.lastname: Donnelly
authors.forename: Y A
authors.initials: YA
authors.lastname: Barnett
authors.forename: W
authors.initials: W
authors.lastname: McCullough
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004249:DNA Damage; D006358:Hot Temperature; D009154:Mutation; D018384:Oxidative Stress; D013172:Spores, Fungal / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D000596:Amino Acids; D015112:4-Nitroquinoline-1-oxide