title: Behavioral and anatomical deficits in mice homozygous for a modified beta-amyloid precursor protein gene.
abstract: The beta-amyloid precursor protein (beta APP) gene of the mouse was disrupted by inserting into exon 2 a cassette containing a neomycin resistance gene and a putative transcription termination sequence. Contrary to expectation, brain and other tissues from mice homozygous for the insertion still contained beta APP-specific RNA, albeit at a level 5- to 10-fold lower than wild type and lacking the disrupted exon, which had been spliced out. The brain contained shortened beta APP-specific protein at a low level. Mutant mice were severely impaired in spatial learning and exploratory behavior and showed increased incidence of agenesis of the corpus callosum.
authors.affiliation: Institut für Molekularbiologie I, Universität Zürich, Switzerland.
authors.forename: U
authors.initials: U
authors.lastname: Müller
authors.forename: N
authors.initials: N
authors.lastname: Cristina
authors.forename: Z W
authors.initials: ZW
authors.lastname: Li
authors.forename: D P
authors.initials: DP
authors.lastname: Wolfer
authors.forename: H P
authors.initials: HP
authors.lastname: Lipp
authors.forename: T
authors.initials: T
authors.lastname: Rülicke
authors.forename: S
authors.initials: S
authors.lastname: Brandner
authors.forename: A
authors.initials: A
authors.lastname: Aguzzi
authors.forename: C
authors.initials: C
authors.lastname: Weissmann
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001522:Behavior, Animal; D001921:Brain / Q000473:pathology*; D005091:Exons / Q000235:genetics; D005106:Exploratory Behavior; D005260:Female; D015870:Gene Expression; D006720:Homozygote; D007858:Learning; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012333:RNA, Messenger / Q000032:analysis; D013037:Spatial Behavior
chemical_list: D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger