Identification of carboxypeptidase H transcripts by antisense RNA.
Carboxypeptidase H is a metallopeptidase involved in the processing of neuropeptide precursors and prohormones. In this study, northern analysis with antisense CPH RNA as probe detected the presence of three CPH mRNA transcripts of 2.2, 2.6, and 5.9 kb that are expressed in a tissue-specific manner in several species. Specifically, mouse brain and the mouse AtT-20 corticotroph cell line possess all three transcripts, but mouse pituitary and adrenal possess the 2.2 kb CPH mRNA as the main form. In bovine, the 5.9 kb CPH mRNA was detected in adrenal medulla, but not in pituitary; whereas, both of these bovine tissues possess the 2.2 kb form. Also, in rat brain, adrenal, heart, and pituitary, the 5.9 m,kb form was detected mainly in brain, and the 2.6 kb form was most abundant in pituitary; all rat tissues examined possessed the 2.2 kb form. Additional evidence for the existence of the largest CPH transcript was provided from a bovine adrenal medulla cDNA library by in vitro transcription of total library phage DNA, followed by analysis of resultant RNAs by Northern blot. It will be important in future studies to define the structural similarities and differences among these multiple CPH mRNA transcripts that are expressed in a tissue-specific manner.
Hwang
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SR
Department of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
Hook
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D000313:Adrenal Medulla / Q000737:chemistry; D000818:Animals; D016375:Antisense Elements (Genetics); D015152:Blotting, Northern; D043423:Carboxypeptidase H; D002268:Carboxypeptidases / Q000235:genetics*; D002417:Cattle; D015698:Genomic Library; D051379:Mice; D010902:Pituitary Gland / Q000737:chemistry; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats
D016375:Antisense Elements (Genetics); D016372:RNA, Antisense; D012333:RNA, Messenger; D002268:Carboxypeptidases; D043423:Carboxypeptidase H