title: Nanosecond absorption study of kinetics associated with carbon monoxide rebinding to hemoglobin S and hemoglobin C following ligand photolysis.
abstract: The absorption spectra of photolysis intermediates of the CO complex of hemoglobin S and hemoglobin C, in the tetramer form, have been measured between 10 ns and 200 ms after excitation. These data were analyzed using singular value decomposition (SVD) and global analysis to determine kinetic lifetimes associated with various processes involved in CO recombination. The results of this analysis show that, in the tetramer (non-aggregated) form, hemoglobin S and hemoglobin C exhibit the same kinetics associated with CO recombination as hemoglobin A.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064.
authors.forename: D B
authors.initials: DB
authors.lastname: Shapiro
authors.forename: S J
authors.initials: SJ
authors.lastname: Paquette
authors.forename: R M
authors.initials: RM
authors.lastname: Esquerra
authors.forename: D
authors.initials: D
authors.lastname: Che
authors.forename: R A
authors.initials: RA
authors.lastname: Goldbeck
authors.forename: R E
authors.initials: RE
authors.lastname: Hirsch
authors.forename: N
authors.initials: N
authors.lastname: Mohandas
authors.forename: D S
authors.initials: DS
authors.lastname: Kliger
mesh_terms: D000042:Absorption; D002248:Carbon Monoxide / Q000378:metabolism*; D006444:Hemoglobin C / Q000378:metabolism*; D006451:Hemoglobin, Sickle / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008024:Ligands; D010782:Photolysis; D013057:Spectrum Analysis
chemical_list: D006451:Hemoglobin, Sickle; D008024:Ligands; D002248:Carbon Monoxide; D006444:Hemoglobin C