Localization of transglutaminase-reactive glutamine residues in bovine osteopontin.
Here we report the identification of two transglutaminase-reactive glutamines (Gln-34 and Gln-36) in bovine osteopontin (OPN). Sequence alignment revealed that these glutamines are conserved in all known OPN sequences, indicating a functional importance of this region of the protein. Furthermore, immunological analysis of bovine bone demonstrated that OPN is present in high-molecular-mass complexes in vivo. These findings support the functional aspects of a transglutaminase-catalysed cross-linking of OPN in facilitating cellular attachment and tissue calcification.
Sørensen
E S
ES
Protein Chemistry Laboratory, University of Aarhus, Denmark.
Rasmussen
L K
LK
Møller
L
L
Jensen
P H
PH
Højrup
P
P
Petersen
T E
TE
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D005973:Glutamine / Q000737:chemistry*; D008969:Molecular Sequence Data; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000737:chemistry*; D013057:Spectrum Analysis; D011503:Transglutaminases / Q000737:chemistry*
D012795:Sialoglycoproteins; D005973:Glutamine; D053495:Osteopontin; D011503:Transglutaminases