Progesterone metabolism by the filamentous fungus Cochliobolus lunatus.
Studies of Cochliobolus lunatus m118 steroid metabolism by thin-layer chromatography, mass spectrometry and NMR spectroscopy revealed that the fungus hydroxylates progesterone at positions 7 alpha, 11 beta and 14 alpha, and oxidizes the 11 beta-hydroxy group to the ketone. The 1H NMR spectra of two of the steroid metabolites, 11 beta,14 alpha-dihydroxyprogesterone and 11-oxo-14 alpha-hydroxyprogesterone, are reported for the first time. It is still not known if all the hydroxylation reactions are performed in C. lunatus by a single, non-specific, steroid hydroxylase, structurally different from the 11 beta-hydroxylase found in higher eucaryotes, or if different forms of the enzyme are involved.
Vita
M
M
Institute of Biochemistry, Medical Faculty, Ljubljana, Slovenia.
Smith
K
K
Rozman
D
D
Komel
R
R
D001203:Ascomycota / Q000378:metabolism*; D001711:Biotransformation; D006908:Hydroxyprogesterones / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011374:Progesterone / Q000737:chemistry / Q000378:metabolism*; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism
D006908:Hydroxyprogesterones; D011374:Progesterone; D013252:Steroid 11-beta-Hydroxylase