title: Calcium-dependent fusion among endosomes.
abstract: Fusion among endosomes is an important step for transport and sorting of internalized macromolecules. Working in a cell-free system, we have previously reported that, in the absence of externally added calcium, endosome fusion requires cytosol, ATP, and is sensitive to N-ethylmaleimide (NEM) and to anti-NEM-sensitive factor (NSF) antibody. This cytosol-dependent fusion is regulated by monomeric and heterotrimeric GTP-binding proteins. Further studies have revealed, however, that in the presence of micromolar concentrations of free calcium, fusion is observed even in the absence of cytosol and ATP. At the electron microscope level, Ca(2+)-dependent endosome aggregation and fusion were similar to that observed for cytosol-dependent fusion. Calcium-dependent fusion was not affected by non-hydrolyzable analogs of GTP or GDP nor by NEM or anti-NSF antibody. However, Ca(2+)-dependent fusion was abrogated by trypsin treatment of the vesicles or by a membrane wash with 60 mM EDTA indicating that peripheral proteins are required. An anti-annexin II antibody and an annexin II peptide blocked Ca(2+)-dependent fusion by 50%. After the EDTA wash, Ca(2+)-dependent fusion was reconstituted by addition of purified annexin II and arachidonic acid. We conclude that endosomes can fuse by two mechanisms, one that has an absolute requirement for calcium and is probably mediated by annexins, and another that does not require calcium.
authors.affiliation: Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo-CONICET, Mendoza, Argentina.
authors.forename: L S
authors.initials: LS
authors.lastname: Mayorga
authors.forename: W
authors.initials: W
authors.lastname: Berón
authors.forename: M N
authors.initials: MN
authors.lastname: Sarrouf
authors.forename: M I
authors.initials: MI
authors.lastname: Colombo
authors.forename: C
authors.initials: C
authors.lastname: Creutz
authors.forename: P D
authors.initials: PD
authors.lastname: Stahl
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000276:immunology; D017306:Annexin A2 / Q000276:immunology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000494:pharmacology; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D010741:Phospholipases A / Q000037:antagonists & inhibitors
chemical_list: D017305:Annexin A1; D017306:Annexin A2; D006150:Guanine Nucleotides; D008565:Membrane Proteins; D010741:Phospholipases A; D002118:Calcium