Characteristics of Hb-vesicles and encapsulation procedure.
The performance of Hb-vesicles depends on the weight ratio of Hb to lipid ([Hb]/[Lipid]). This value is improved by lowering the number of bilayer membrane of the vesicle and raising the concentration of Hb in the interior of the vesicle. Maximum [Hb]/[Lipid] ratio was obtained at ca. pH 7, that would relate to the isoelectric point (pI) of Hb at 25 degrees C. On the other hand, the [Hb]/[Lipid] ratio decreased with ionic strength and increased with lowering temperature. The Hb-vesicles encapsulating 40 g/dl Hb with only one bilayer membrane were isolated by using the difference in the density of the vesicles.
Takeoka
S
S
Department of Polymer Chemistry, Waseda University, Tokyo, Japan.
Sakai
H
H
Terase
K
K
Nishide
H
H
Tsuchida
E
E
D001802:Blood Substitutes / Q000008:administration & dosage* / Q000737:chemistry; D006454:Hemoglobins / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007526:Isoelectric Point; D008081:Liposomes; D009994:Osmolar Concentration; D012996:Solutions; D013696:Temperature
D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes; D012996:Solutions; C016484:hemoglobin, stroma free