title: Conjugation reactions of cyclodepsipeptide to glutathionyl adducts by direct 'in-beam' synthesis under negative-ion fast-atom bombardment conditions.
abstract: The conjugation products of E-destruxin (cyclodepsipeptide) with glutathione have been identified by fast-atom bombardment mass spectrometry. Appropriate conditions were developed for cyclodepsipeptide substrate, which enabled the direct, dynamic mass spectra analysis of spontaneous as well as glutathione-s-transferase catalyzed conjugation reactions. Application to the most active cyclopeptide in the series of destruxins yielded glutathionyl adduct.
authors.affiliation: Département de Chimie, Université de Versailles, France.
authors.forename: C
authors.initials: C
authors.lastname: Loutelier
authors.forename: J C
authors.initials: JC
authors.lastname: Cherton
authors.forename: C
authors.initials: C
authors.lastname: Lange
mesh_terms: D000595:Amino Acid Sequence; D047630:Depsipeptides; D005656:Fungal Proteins; D005978:Glutathione / Q000737:chemistry*; D005982:Glutathione Transferase / Q000378:metabolism; D003904:Mitosporic Fungi / Q000737:chemistry; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D047630:Depsipeptides; D005656:Fungal Proteins; D009183:Mycotoxins; D010456:Peptides, Cyclic; D005982:Glutathione Transferase; D005978:Glutathione; C040425:destruxin E