title: Glyoxylic acid production using immobilized glycolate oxidase and catalase.
abstract: A variety of methods for the immobilization of glycolate oxidase have been examined for the preparation of a catalyst for the oxidation of glycolic acid to glyoxylic acid. The co-immobilization of glycolate oxidase and catalase on oxirane acrylic beads produced a catalyst which was stable to the reaction conditions used for the oxidation, where glycolic acid and oxygen are reacted in aqueous solution in the presence of the immobilized enzyme catalyst and ethylenediamine. Under optimum reaction conditions, 99% yields of glyoxylic acid were obtained at greater than 99% conversion of glycolic acid, and the recovery and reuse of the co-immobilized enzyme catalyst was demonstrated.
authors.affiliation: Central Research and Development Department, E. I. du Pont de Nemours & Co., Wilmington, DE 19880-0328.
authors.forename: J E
authors.initials: JE
authors.lastname: Seip
authors.forename: S K
authors.initials: SK
authors.lastname: Fager
authors.forename: J E
authors.initials: JE
authors.lastname: Gavagan
authors.forename: D L
authors.initials: DL
authors.lastname: Anton
authors.forename: R
authors.initials: R
authors.lastname: Di Cosimo
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D001234:Aspergillus niger / Q000201:enzymology; D002374:Catalase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006038:Glyoxylates / Q000138:chemical synthesis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D018724:Spinacia oleracea / Q000201:enzymology; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D006038:Glyoxylates; D000429:Alcohol Oxidoreductases; C020821:glycollate oxidase; D002374:Catalase; C031150:glyoxylic acid