title: Properties of Yersinia enterocolitica porins: interference with biological functions of phagocytes, nitric oxide production and selective cytokine release.
abstract: We have extracted and purified Yersinia enterocolitica ATCC 9610 porins that have molecular weights of 36-38 kDa. They inhibited phagocytosis and phagosome-lysosome fusion (30%) in human monocytes and caused enhanced nitrite production. Preincubation of polymorphonuclear neutrophils with porins (1-10 micrograms/ml/10(6) cells) induced a reduction in chemotaxis, adherence to nylon wool and chemiluminescence. Human lymphomonocytes treated with Y. enterocolitica porins showed a distinctive cytokine profile. Interleukin-1 alpha, interleukin-6 and tumour necrosis factor alpha were released within 3-6 h, while interleukin-8, gamma interferon and granulocyte-macrophage colony stimulating factor were released after 18 h. Interleukin-3 and interleukin-4 were not detected at up to 48 h of incubation. In conclusion, these immunomodulating and histotropic properties may account for Y. enterocolitica infection and its sequelae.
authors.affiliation: Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, Italy.
authors.forename: M A
authors.initials: MA
authors.lastname: Tufano
authors.forename: F
authors.initials: F
authors.lastname: Rossano
authors.forename: P
authors.initials: P
authors.lastname: Catalanotti
authors.forename: G
authors.initials: G
authors.lastname: Liguori
authors.forename: A
authors.initials: A
authors.lastname: Marinelli
authors.forename: A
authors.initials: A
authors.lastname: Baroni
authors.forename: P
authors.initials: P
authors.lastname: Marinelli
mesh_terms: D002633:Chemotaxis / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects; D009566:Nitrates / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects*; D018272:Porins / Q000096:biosynthesis / Q000494:pharmacology*; D015008:Yersinia enterocolitica / Q000378:metabolism*
chemical_list: D016207:Cytokines; D009566:Nitrates; D018272:Porins