Expressed mu opiate receptor couples to adenylate cyclase and phosphatidyl inositol turnover.
The recently cloned rat mu opiate receptor cDNA has been expressed in COS and Chinese hamster ovary (CHO) cells to examine the coupling of this receptor to G-protein linked second messenger systems and examine possible coupling to multiple second messenger systems. Morphine (1 microM) reduced both forskolin-stimulated cAMP levels and IP3 levels by 20 +/- 5 and 34 +/- 8% respectively in COS and CHO cell cultures expressing the cloned rat mu receptor cDNA. Both effects could be blocked by naloxone and Gpp(NH)p. These results represent the first clear representation of the second messenger system promiscuity possible with a single cloned opiate receptor.
Johnson
P S
PS
Molecular Neurobiology Branch, National Institute on Drug Abuse, NIH, Baltimore, MD.
Wang
J B
JB
Wang
W F
WF
Uhl
G R
GR
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D003001:Cloning, Molecular; D005576:Colforsin / Q000494:pharmacology; D006224:Cricetinae; D018076:DNA, Complementary / Q000096:biosynthesis; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D009020:Morphine / Q000494:pharmacology; D009270:Naloxone / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D017450:Receptors, Opioid, mu / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D015290:Second Messenger Systems / Q000502:physiology*
D018076:DNA, Complementary; D010716:Phosphatidylinositols; D017450:Receptors, Opioid, mu; D011994:Recombinant Proteins; D005576:Colforsin; D006165:Guanylyl Imidodiphosphate; D009270:Naloxone; D009020:Morphine; D000262:Adenylyl Cyclases