The use of anticlusterin monoclonal antibodies for the combined assessment of human sperm morphology and acrosome integrity.
Clusterin is an abundant protein in the human male reproductive tract which appears to be produced by the testis, epididymis and the seminal vesicles. Using monoclonal antibodies and an amplified immunoperoxidase technique, we have identified two apparently biochemically distinct forms of clusterin on human spermatozoa. Morphologically abnormal spermatozoa have an extensive surface coating of conventional 80 kDa native clusterin, but this form of clusterin is not detectable on normal spermatozoa. Normal spermatozoa, however, contain within the acrosomal cap a different form of clusterin, reactive with an anticlusterin alpha-chain antibody. Agglutinated spermatozoa, most of which are grossly abnormal, were intensely labelled with the antibody against conventional 80 kDa clusterin, suggesting that the 'clustering' properties of this protein may play a role in the aggregation of abnormal spermatozoa. Anticlusterin monoclonal antibodies may be useful for semen analysis. Staining spermatozoa with anticlusterin monoclonal antibodies is a technically simple method which provides a visually obvious means of assessing spermatozoa morphology and acrosome status simultaneously. The current data also suggest that different functions of clusterin in the reproductive tract may be attributed to different molecular forms of the protein.
O'Bryan
M K
MK
Department of Medicine (St Vincent's Hospital), University of Melbourne, Parkville, Australia.
Murphy
B F
BF
Liu
D Y
DY
Clarke
G N
GN
Baker
H W
HW
D000177:Acrosome / Q000187:drug effects / Q000502:physiology*; D000911:Antibodies, Monoclonal; D000001:Calcimycin / Q000494:pharmacology; D051152:Clusterin; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D018832:Molecular Chaperones; D008970:Molecular Weight; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities* / Q000737:chemistry / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; C495242:CLU protein, human; D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; D000001:Calcimycin