Evaluation of a new citrate-acetate-NaCl platelet additive solution for the storage of white cell-reduced platelet concentrates obtained from half-strength CPD pooled buffy coats.
BACKGROUND
A new citrate-acetate-NaCl platelet additive solution, identified as PAS 2, was developed to prepare platelet concentrates (PCs) from pooled 0.5 CPD buffy coats (BCs).


METHODS
A study was undertaken to evaluate PAS 2 in vitro (n = 8) and in vivo (n = 9) against a commercially available solution (Plasma-Lyte A). In a paired in vitro study, a comparison was made of platelet and white cell concentration; blood gases and bicarbonate; glucose and lactate concentration; total intracellular concentration of adenine nucleotides and beta-thromboglobulin release.


RESULTS
A lower platelet yield (p < 0.0001) and a higher beta-thromboglobulin release (p < 0.01) are observed with Plasma-Lyte A. For this reason, half-strength (0.5) CPD was changed to full-strength CPD in the clinical study with Plasma-Lyte A. In a clinical evaluation of nine patients with bone marrow failure, all received PCs with both PAS 2 and Plasma-Lyte A that had a shelf life of at least 4 days. Corrected count increments (CCls) were as follows, on average (95% Cl): the CCl at 1 to 4 hours was 22.4 (95% Cl, 15.2-29.4) for PAS 2 and 24.0 (95% Cl, 16.9-31.2) for Plasma-Lyte A; that at 12 to 24 hours was 11.3 (95% Cl, 4.1-18.4) for PAS 2 and 14.2 (95% Cl, 7.1-21.3) for Plasma-Lyte A; and that at 36 to 48 hours was 4.2 (95% Cl, -3.0-11.3) for PAS 2 and 8.7 (95% Cl, 1.1-16.2) for Plasma-Lyte A. No significant difference between the two solutions was found.


CONCLUSIONS
PAS 2 and Plasma-Lyte A make important contributions to platelet transfusion quality improvement and give an excellent CCl even after 4 days of storage.
van Rhenen
D J
DJ
Red Cross Bloodbank, Rotterdam, the Netherlands.
Vermeij
H
H
Kappers-Klunne
M
M
Payrat
J M
JM
D000085:Acetates; D000227:Adenine Nucleotides / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001792:Blood Platelets; D001793:Blood Preservation; D002469:Cell Separation; D002951:Citrates; D005069:Evaluation Studies as Topic; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology*; D010976:Platelet Count; D012965:Sodium Chloride; D012996:Solutions; D001620:beta-Thromboglobulin / Q000378:metabolism
D000085:Acetates; D000227:Adenine Nucleotides; D002951:Citrates; D007773:Lactates; D012996:Solutions; D001620:beta-Thromboglobulin; D012965:Sodium Chloride; D000255:Adenosine Triphosphate; D005947:Glucose