title: Detection of avocado sunblotch viroid in chloroplasts of avocado leaves by in situ hybridization.
abstract: In situ hybridization experiments were carried out to detect avocado sunblotch viroid (ASBVd) in foliar tissue of avocado, using a digoxigenin-labelled RNA probe complementary to the ASBVd-RNA in sections of aldehyde-fixed, LRGold-embedded leaf samples. Detection of the probe was made through anti-digoxigenin antibody and protein-A colloidal gold (20 nm). Seventy to 80% of the signals came from chloroplast while the cytoplasm and vacuole were labelled with ca. 10% of the gold particles. This is in contrast with the subcellular localization of potato spindle tuber viroid and some other related viroids, which are mainly found in the nucleus.
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brazil.
authors.forename: M I
authors.initials: MI
authors.lastname: Lima
authors.forename: M E
authors.initials: ME
authors.lastname: Fonseca
authors.forename: R
authors.initials: R
authors.lastname: Flores
authors.forename: E W
authors.initials: EW
authors.lastname: Kitajima
mesh_terms: D002736:Chloroplasts / Q000821:virology*; D005638:Fruit / Q000821:virology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015388:Organelles / Q000821:virology; D015347:RNA Probes; D012367:RNA, Viral / Q000032:analysis*; D014772:Viroids / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D015347:RNA Probes; D012367:RNA, Viral