Immobilization of Escherichia coli expressing the lux genes of Xenorhabdus luminescens.
The luxCDABE operon of Xenorhabdus luminescens was cloned into pUC18 to make pLITE27. Expression of the lux genes from the lac promoter resulted in strong constitutive light emission by Escherichia coli DH5 carrying the recombinant lux plasmid, pLITE27. When strain DH5(pLITE27) was immobilized with sodium alginate-CaCl2, the embedded cells retained their luminescence up to 2 weeks under appropriate storage conditions.
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Plant Molecular Genetics Laboratory, Grasslands Research Centre, Palmerston North, New Zealand.
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D003001:Cloning, Molecular; D003470:Culture Media; D004755:Enterobacteriaceae / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D049449:Luminescence
D003470:Culture Media