title: Comparison of polyacrylamide gel electrophoresis & silver staining method with ELISA for rotavirus detection.
abstract: A total of 421 human fecal samples were examined for the presence of rotavirus by enzyme-linked immunosorbent assay (ELISA) and by a modified polyacrylamide gel electrophoretic (PAGE) analysis of viral genome. Similar results by both methods were seen in 412 (97.86%) samples. The proportion of ELISA + PAGE - samples (2/421) was lower than the proportion of ELISA - PAGE + samples (7/421). This difference was not significant. The modified PAGE technique for the detection of viral RNA was found to be a rapid, simple, reliable and less expensive technique.
authors.affiliation: Department of Microbiology, Maulana Azad Medical College, New Delhi.
authors.forename: A
authors.initials: A
authors.lastname: Chakravarti
authors.forename: Z
authors.initials: Z
authors.lastname: Hakim
mesh_terms: D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D012401:Rotavirus / Q000302:isolation & purification*; D016622:Silver Staining