title: Growth of Legionella and other heterotrophic bacteria in a circulating cooling water system exposed to ultraviolet irradiation.
abstract: The effect of ultraviolet irradiation on the growth and occurrence of legionella and other heterotrophic bacteria in a circulating cooling water system was studied. Water of the reservoir was circulated once in 28 h through a side-stream open channel u.v. radiator consisting of two lamps. Viable counts of legionellas and heterotrophic bacteria in water immediately after the u.v. treatment were 0-12 and 0.7-1.2% of those in the reservoir, respectively. U.v. irradiation increased the concentration of easily assimilable organic carbon. In the u.v. irradiated water samples incubated in the laboratory the viable counts of heterotrophic bacteria reached the counts in reservoir water within 5 d. The increase in viable counts was mainly due to reactivation of bacterial cells damaged by u.v. light, not because of bacterial multiplication. Despite u.v. irradiation the bacterial numbers in the reservoir water, including legionellas, did not decrease during the experimental period of 33 d. The main growth of bacteria in the reservoir occurred in biofilm and sediment, which were never exposed to u.v. irradiation.
authors.affiliation: Department of Environmental Microbiology, National Public Health Institute, Kuopio, Finland.
authors.forename: J M
authors.initials: JM
authors.lastname: Kusnetsov
authors.forename: P J
authors.initials: PJ
authors.lastname: Keskitalo
authors.forename: H E
authors.initials: HE
authors.lastname: Ahonen
authors.forename: A I
authors.initials: AI
authors.lastname: Tulkki
authors.forename: I T
authors.initials: IT
authors.lastname: Miettinen
authors.forename: P J
authors.initials: PJ
authors.lastname: Martikanen
mesh_terms: D000389:Air Conditioning / Q000009:adverse effects; D001419:Bacteria / Q000254:growth & development* / Q000302:isolation & purification / Q000528:radiation effects*; D002244:Carbon / Q000378:metabolism; D015169:Colony Count, Microbial; D004197:Disease Reservoirs; D007875:Legionella / Q000254:growth & development* / Q000302:isolation & purification / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014871:Water Microbiology
chemical_list: D002244:Carbon