Identification and activity of cytosol creatine phosphokinase enzymes in normal and diseased skin.
Phosphocreatine molecules (PCR) in skin regenerate adenosine triphosphate and help cutaneous tissue survive ischemia associated with skin flaps, grafts, and hair transplantation procedures. In addition, PCR concentration in psoriasis is elevated many times above normal, indicating either overproduction of PCR by mitochondrial creatine phosphokinase (CPK) enzymes or a defect in cytosol CPK enzymatic activity. Skin CPK isoenzymes, before this study, have not been identified. Herein, for the first time, cytosol CPK enzymatic activity was measured in normal and psoriatic, involved and uninvolved skin, skin tumors, and mouse skin and keratinocyte cell cultures. Creatine phosphokinase MM is the major isoenzyme in normal, uninvolved psoriatic and mouse skin. Total CPK enzymatic activity was increased in psoriasis and skin tumors. These data clearly indicate that increased PCR concentration in a psoriatic skin is not a result of decreased cytosol CPK enzymatic activity.
Zemtsov
A
A
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine.
Cameron
G S
GS
Bradley
C A
CA
Montalvo-Lugo
V
V
Mattioli
F
F
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002460:Cell Line; D003402:Creatine Kinase / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D004734:Energy Metabolism; D006801:Humans; D007527:Isoenzymes; D015603:Keratinocytes / Q000201:enzymology; D008297:Male; D051379:Mice; D010725:Phosphocreatine / Q000378:metabolism; D011565:Psoriasis / Q000201:enzymology; D012867:Skin / Q000201:enzymology* / Q000378:metabolism; D012871:Skin Diseases / Q000201:enzymology* / Q000378:metabolism; D012878:Skin Neoplasms / Q000201:enzymology
D007527:Isoenzymes; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D003402:Creatine Kinase