[Nasal reactivity in anesthetized guinea pigs].
The authors elaborated a methodical procedure for observing the nasal reactivity in anaesthetized guinea pigs (Pentobarbital Spofa, 32 mg.kg-1 i.p.): Volume changes of the nasal mucosa were estimated from the changes in nasal airflow resistance (Rn). Via tracheostomic aperture the authors introduced a cannula into the nasopharynx. By the use of the cannula they drove doses of continual airflow through the nasal cavities, the airflow doses being 0.5, 1.0, 1.5, thereafter in reversed order: 1.0 and 0.5 l.min-1. Contemporaneously they registered the nasopharyngeal pressure. Rn represented the quotient of the transnasal pressure (the difference between the nasopharyngeal and atmospheric pressure) and the relevant airflow. They filled the nasal cavities for three minutes with histamine solution, the concentration of which was doubled following each application, gradually from 0.625 to 2.0 g.l-1. They discovered that the average values of Rn increase in dependence of histamine doses. Maximal response of the nasal mucosa was detected in between the doses of 0.5-1.0 g.l-1. For estimation of the threshold dose it is necessary to observe still lower histamine concentrations. (Fig. 1, Ref. 14.).
Tatár
M
M
Ustav patologickej fyziológie LFUK, Martine, Slovakia.
Nikel
J
J
Mráz
R
R
D000403:Airway Resistance; D000758:Anesthesia; D000818:Animals; D006168:Guinea Pigs; D006632:Histamine; D008297:Male; D009299:Nasal Provocation Tests; D010424:Pentobarbital
D006632:Histamine; D010424:Pentobarbital
Sledovanie nazálnej reaktivity u anestézovaných morciat.