Evaluation of the E test for antimicrobial susceptibility testing of Porphyromonas gingivalis.
The antimicrobial susceptibilities of 81 recent clinical Porphyromonas gingivalis isolates and two reference strains were determined by the E test, a new method, and were compared with the minimal inhibitory concentrations for these strains obtained by the reference agar dilution method on supplemented Brucella blood agar. The following agreements were obtained: benzylpenicillin 100%, ampicillin 96%, cefaclor 82%, cefuroxime 91%, erythromycin 93%, clindamycin 99%, tetracycline 66%, doxycycline 89%, metronidazole 77% and ciprofloxacin 77%. Very major discrepancies were observed with ciprofloxacin. This study indicates that the E test is an acceptable method to determine the susceptibility of P. gingivalis for most antimicrobials.
Pajukanta
R
R
Department of Periodontology, University of Helsinki, Finland.
Asikainen
S
S
Forsblom
B
B
Piekkola
M
M
Jousimies-Somer
H
H
D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002433:Cefaclor / Q000494:pharmacology; D002444:Cefuroxime / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D002981:Clindamycin / Q000494:pharmacology; D015169:Colony Count, Microbial; D004318:Doxycycline / Q000494:pharmacology; D004917:Erythromycin / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010400:Penicillin G / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D013754:Tetracyclines / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D013754:Tetracyclines; D008795:Metronidazole; D002981:Clindamycin; D002939:Ciprofloxacin; D004917:Erythromycin; D002433:Cefaclor; D000667:Ampicillin; D004318:Doxycycline; D002444:Cefuroxime; D010400:Penicillin G