Crystallization of enzyme IIB of the cellobiose-specific phosphotransferase system of Escherichia coli.
Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4 degrees. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 A resolution and have the monoclinic space group P2(1), with cell dimensions a = 53.6 A, b = 31.7 A, c = 60.0 A and beta = 101.7 degrees. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.
van Montfort
R L
RL
Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands.
Pijning
T
T
Kalk
K H
KH
Schuurman-Wolters
G K
GK
Reizer
J
J
Saier
M H
MH
Robillard
G
G
Dijkstra
B W
BW
D002475:Cellobiose; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry*
D002475:Cellobiose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System