title: The BE (2)-M17 neuroblastoma cell line synthesizes and secretes corticotropin-releasing factor.
abstract: The BE (2)-M17 human neuroblastoma has previously been shown to express corticotropin-releasing factor (CRF) mRNA following retinoic acid treatment. It is demonstrated in this report that both cell extracts and cell incubation medium of retinoic acid-treated BE (2)-M17 cells were shown to contain CRF-like immunoreactivity (CRF-LI) by RIA. CRF-LI secretion and content were also dose-dependently increased by forskolin. In addition, cell extracts were applied to a C18 Vydac column and peak CRF-LI from the collected fractions was shown to coincide in time of elution with peak immunoreactivity seen with oxidized synthetic CRF standard. Thus, in containing the CRF peptide, the BE (2)-M17 cells are useful models for further study of CRF cellular and genetic regulation.
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Emory University Medical Center, Atlanta, GA 30322.
authors.forename: J W
authors.initials: JW
authors.lastname: Kasckow
authors.forename: D G
authors.initials: DG
authors.lastname: Parkes
authors.forename: M J
authors.initials: MJ
authors.lastname: Owens
authors.forename: M D
authors.initials: MD
authors.lastname: Stipetic
authors.forename: J H
authors.initials: JH
authors.lastname: Han
authors.forename: C B
authors.initials: CB
authors.lastname: Nemeroff
authors.forename: W W
authors.initials: WW
authors.lastname: Vale
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005576:Colforsin / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism*; D011863:Radioimmunoassay; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D005576:Colforsin; D014212:Tretinoin; D003346:Corticotropin-Releasing Hormone