Motor cortex and pyramidal tract axons responsible for electrically evoked forelimb flexion: refractory periods and conduction velocities.
Double-pulse methods are used here to measure the refractory periods and conduction velocities of the pyramidal tract axons which cause forelimb flexion in pentobarbital anesthetized rats. In the refractory period experiments, conditioning and test pulses were delivered to the motor cortex, the ipsilateral internal capsule, or the ipsilateral pyramid, and the maximum force exerted by the contralateral forelimb was measured at various conditioning-test intervals. The movements increased as conditioning-test interval increased from 0.5 to 1.0 ms in pyramid sites, from 0.6 to 1.5 in internal capsule sites, and from 0.6 to 2.0 ms in surface cortical sites, suggesting longer refractory periods for the substrates at more rostral sites. In cortical sites, as the conditioning-test interval increased from 4.0 to 20.0 ms, the movements decreased gradually to the single-pulse level, suggesting decreasing temporal summation at longer conditioning-test intervals. In the collision experiments, when conditioning pulses were delivered to one site and test pulses to a second site, the movements increased at conditioning-test intervals that were longer by 0.5-1.3 ms than the refractory periods in either site. This suggests that collisions occurred between orthodromic and antidromic action potentials in the pyramidal tract axons responsible for the limb movement. The collision-like increase was greater between internal capsule and pyramid than between cortex and pyramid, or between cortex and internal capsule. The estimated conduction times were 0.9-1.5 ms between cortex and pyramid, 0.4-0.8 ms between cortex and internal capsule, and 0.5-0.8 ms between internal capsule and pyramid. The range of conduction velocities, therefore, was quite narrow between all pairs (8.8-16.8 m/s). The largest pyramidal tract axons appear to be responsible for most of the force of forelimb flexion in pentobarbital anesthetized rats.
Chapman
C A
CA
Department of Psychology, University of Toronto, Canada.
Yeomans
J S
JS
D000818:Animals; D001369:Axons / Q000502:physiology*; D003213:Conditioning, Psychological; D004558:Electric Stimulation; D005552:Forelimb / Q000294:innervation*; D008297:Male; D009043:Motor Activity; D009044:Motor Cortex / Q000502:physiology*; D009068:Movement; D009431:Neural Conduction; D011712:Pyramidal Tracts / Q000502:physiology*; D051381:Rats; D013997:Time Factors