Lipoic acid metabolism in Escherichia coli: the lplA and lipB genes define redundant pathways for ligation of lipoyl groups to apoprotein.
Lipoic acid is a covalently bound disulfide-containing cofactor required for function of the pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase, and glycine cleavage enzyme complexes of Escherichia coli. Recently we described the isolation of the lplA locus, the first gene known to encode a lipoyl-protein ligase for the attachment of lipoyl groups to lipoate-dependent apoenzymes (T. W. Morris, K. E. Reed, and J. E. Cronan, Jr., J. Biol. Chem. 269:16091-16100, 1994). Here, we report an unexpected redundancy between the functions of lplA and lipB, a gene previously identified as a putative lipoate biosynthetic locus. First, analysis of lplA null mutants revealed the existence of a second lipoyl ligase enzyme. We found that lplA null mutants displayed no growth defects unless combined with lipA (lipoate synthesis) or lipB mutations and that overexpression of wild-type LplA suppressed lipB null mutations. Assays of growth, transport, lipoyl-protein content, and apoprotein modification demonstrated that lplA encoded a ligase for the incorporation of exogenously supplied lipoate, whereas lipB was required for function of the second lipoyl ligase, which utilizes lipoyl groups generated via endogenous (lipA-mediated) biosynthesis. The lipB-dependent ligase was further shown to cause the accumulation of aberrantly modified octanoyl-proteins in lipoate-deficient cells. Lipoate uptake assays of strains that overproduced lipoate-accepting apoproteins also demonstrated coupling between transport and the subsequent ligation of lipoate to apoprotein by the LplA enzyme. Although mutations in two genes (fadD and fadL) involved in fatty acid failed to affect lipoate utilization, disruption of the smp gene severely decreased lipoate utilization. DNA sequencing of the previously identified slr1 selenolipoate resistance mutation (K. E. Reed, T. W. Morris, and J. E. Cronan, Jr., Proc. Natl. Acad. Sci. USA 91:3720-3724, 1994) showed this mutation (now called lplA1) to be a G76S substitution in the LplA ligase. When compared with the wild-type allele, the cloned lplA1 allele conferred a threefold increase in the ability to discriminate against the selenium-containing analog. These results support a two-pathway/two-ligase model of lipoate metabolism in E. coli.
Morris
T W
TW
Department of Microbiology, University of Illinois at Urbana-Champaign 61801.
Reed
K E
KE
Cronan
J E
JE
D000595:Amino Acid Sequence; D001051:Apoenzymes / Q000378:metabolism; D029681:Arabidopsis Proteins; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D002210:Caprylates / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D029968:Escherichia coli Proteins; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism; D050612:Fatty Acid Transport Proteins; D005798:Genes, Bacterial; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation; D010453:Peptide Synthases; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017386:Sequence Homology, Amino Acid; D008063:Thioctic Acid / Q000378:metabolism* / Q000494:pharmacology
D001051:Apoenzymes; D029681:Arabidopsis Proteins; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002210:Caprylates; D029968:Escherichia coli Proteins; D050612:Fatty Acid Transport Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D010940:Plant Proteins; C065691:fadL protein, E coli; C490345:lplA protein, E coli; C089935:lplA protein, bacteria; D008063:Thioctic Acid; D044943:Fatty Acid Desaturases; C084083:Fad7 protein, Arabidopsis; D008025:Ligases; D010453:Peptide Synthases; C072322:lipoate-protein ligase; C031492:octanoic acid