Immunogenicity study of a synthetic T-cell epitope of rubella virus capsid protein recognized by human T cells in different strains of mice.
The immunogenicity of a human immunodominant T-cell epitope C9 (residues 205-233) of rubella virus capsid protein was studied in three strains of mice using C9 lipopeptide. This peptide induced strong T-cell responses in all three strains of mice. The minimal T-cell epitope C9B (residues 205-216) recognized by human T cells with HLA-DR4 phenotype did not specifically stimulate proliferation of T cells from mice in vitro. T cells specific for C9 from immunized mice were shown to be CD4+, in agreement with results of similar studies in RV-seropositive humans.
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Department of Pathology, University of British Columbia, Vancouver, Canada.
Chong
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Gillam
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D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D012412:Rubella virus / Q000276:immunology*; D013045:Species Specificity; D014758:Viral Core Proteins / Q000276:immunology*
D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D014758:Viral Core Proteins; C074573:nucleocapsid protein (C), rubella virus