Actin accumulation associated with clustered and localized adherence in Escherichia coli isolated from patients with diarrhea.
Escherichia coli D2 (serotype 07:H-) that was isolated from a child with diarrhea hybridized with an F1845 DNA probe used to detect diffuse adherence. Strain D2 adhered to tissue culture cells (HeLa and HEp-2 cells) in a clustered pattern but did not autoagglutinate on the cell surface and induced the elongation of microvilli after 3 h of incubation. After 6 h of incubation, the infected cells were positive for fluorescent-actin staining at the site of clustered adherence. When analyzed with a confocal laser scanning microscope, each D2 cell was surrounded by accumulated actin in a capsule-like formation. Capsule-like, accumulated actin was also observed with enteropathogenic E. coli (EPEC), although in this case, actin accumulation was associated with EPEC microcolonies in a localized pattern. Four other strains of F1845 DNA probe-positive, diffusely adhering E. coli were negative for actin accumulation. Strain D2 did not hybridize with EPEC attaching and effacing DNA or EPEC adherence factor DNA probes. In addition, clustered D2 cells were found inside tissue culture cells. The data suggest a novel infectious mechanism as well as genetic heterogeneity of F1845 DNA probe-positive E. coli. Capsule-like, accumulated actin may protect the bacteria from host defense mechanisms.
Yamamoto
T
T
Department of Bacteriology, School of Medicine, Juntendo University, Tokyo, Japan.
Kaneko
M
M
Changchawalit
S
S
Serichantalergs
O
O
Ijuin
S
S
Echeverria
P
P
D000199:Actins / Q000302:isolation & purification* / Q000648:ultrastructure; D001422:Bacterial Adhesion / Q000502:physiology*; D015342:DNA Probes; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D007223:Infant; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008871:Microvilli / Q000648:ultrastructure; D010590:Phalloidine / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D013785:Thailand / Q000453:epidemiology; D014170:Transformation, Genetic; D014407:Tumor Cells, Cultured; D014774:Virulence / Q000235:genetics
D000199:Actins; D015342:DNA Probes; D010590:Phalloidine; D016650:Fluorescein-5-isothiocyanate