title: Genetic evidence that hepA gene is involved in the normal deposition of the envelope of both heterocysts and akinetes in Anabaena variabilis ATCC 29413.
abstract: The hepA gene in Anabaena sp. strain PCC 7120 is required for normal formation of the polysaccharide layer of the heterocyst envelope. A plasmid bearing hepA, interrupted by a neomycin-resistance cassette, was transferred by conjugation to wild type Anabaena variabilis ATCC 29413, so that the interrupted hepA gene replaced a homologous sequence. In the recombinant exconjugants, the envelopes of akinetes as well as of heterocysts were altered.
authors.affiliation: MSU-DOE Plant Research Laboratory, Michigan State University, E. Lansing 48824.
authors.forename: F
authors.initials: F
authors.lastname: Leganés
mesh_terms: D018528:ATP-Binding Cassette Transporters; D017033:Anabaena / Q000235:genetics* / Q000254:growth & development; D016667:Bacterial Capsules / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D011135:Polysaccharides, Bacterial / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; C079727:hepA protein, Anabaena