title: Abdominal rubber drain piece aggravates intra-abdominal sepsis in the rat.
abstract: Biomaterials in the peritoneal cavity disrupt the physiology of the host and may cause bacterial translocation. The current study was performed to determine whether biomaterials exacerbate intra-abdominal infections. Adult male rats were divided into four groups: group 1, celiotomy+intraperitoneal (i.p.) saline; group 2, celiotomy+i.p. Escherichia coli (3 x 10(8) cfu); group 3, i.p. rubber+i.p. saline; and group 4, i.p. rubber+i.p. E. coli (3 x 10(8) cfu). Twelve h after the challenge, enteric bacterial translocation, bacterial population levels in the cecum and serum levels of IL-6 and TNF were measured. Bacterial translocation to mesenteric lymph nodes and the liver was observed in animals from groups 2 and 3, but significantly increased in group 4 with a concomitant elevation of serum levels of TNF and IL-6, as compared with group 1. Histological examination revealed a more pronounced inflammatory reaction in the peritoneum and distal ileum in group 4 than in groups 2 and 3. These results suggest that the presence of rubbers in the peritoneal cavity aggravates intra-abdominal sepsis.
authors.affiliation: Department of Surgery, Lund University, Sweden.
authors.forename: W
authors.initials: W
authors.lastname: Guo
authors.forename: V
authors.initials: V
authors.lastname: Soltesz
authors.forename: J W
authors.initials: JW
authors.lastname: Ding
authors.forename: R
authors.initials: R
authors.lastname: Willén
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: R
authors.initials: R
authors.lastname: Andersson
authors.forename: S
authors.initials: S
authors.lastname: Bengmark
mesh_terms: D000005:Abdomen / Q000601:surgery; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects*; D002465:Cell Movement; D004322:Drainage / Q000009:adverse effects*; D004755:Enterobacteriaceae / Q000472:pathogenicity; D006400:Hematocrit; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D010537:Peritoneum / Q000473:pathology; D010538:Peritonitis / Q000150:complications*; D019736:Prostheses and Implants / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012408:Rubber / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D001672:Biocompatible Materials; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D012408:Rubber