Lysis of blood platelets by human osteosarcoma cells in vitro.
The in vitro interactions between human osteosarcoma (HOS) cells and platelets were studied in real time using video-enhanced microscopy. Interference reflection techniques showed that platelets were lysed within minutes after contacting HOS cells that had been treated with interferon-gamma. Untreated HOS cells lysed platelets less efficiently. Platelet lysis depended on platelet-tumor cell contact and on extracellular Ca2+. A number of possible mechanisms were excluded. Lysis of platelets in proximity to tumor cells can provide these with growth factors and thereby contribute to the metastasis-enhancing effect of platelets.
Hagmann
J
J
Friedrich Miescher Institute, Basel, Switzerland.
Dagan
D
D
Burger
M M
MM
D001792:Blood Platelets / Q000187:drug effects / Q000473:pathology*; D002118:Calcium / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D003199:Computer Systems; D006461:Hemolysis / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008857:Microscopy, Interference / Q000379:methods; D018715:Microscopy, Video; D012516:Osteosarcoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
D007371:Interferon-gamma; D002118:Calcium