Correlation of bioenergetic parameters with cell death in Listeria monocytogenes cells exposed to nisin.
In Listeria monocytogenes, nisin induced ATP efflux, reduced the intracellular ATP concentration within 1 min, and dissipated the proton motive force within 2 min. Efflux accounted for only 20% of the ATP depletion, suggesting that ATP hydrolysis also occurred. ATP efflux depended on nisin concentration and followed saturation kinetics. These results suggest that nisin breaches the membrane permeability barrier in a manner more consistent with pore formation than with a nonspecific detergent-like membrane destabilization.
Winkowski
K
K
Department of Food Science, New Jersey Agricultural Experiment Station, Cook College, Rutgers, State University of New Jersey, New Brunswick 08903.
Bruno
M E
ME
Montville
T J
TJ
D000255:Adenosine Triphosphate / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D009561:Nisin / Q000493:pharmacokinetics*; D011522:Protons
D011522:Protons; D009561:Nisin; D000255:Adenosine Triphosphate