title: Interaction of fibronectin (FN) cell binding fragments and interleukin-8 (IL-8) in regulating neutrophil chemotaxis.
abstract: This study investigated the possible interaction of FN fragments in regulating IL-8-mediated neutrophil chemotaxis in vitro using Neuroprobe microchambers. Human neutrophil suspensions were incubated with purified FN fragments or an RGD-containing peptide and allowed to migrate in response to chemotactically active concentrations of human recombinant IL-8. The 120-kD fragment of FN containing the RGD sequence or an RGD peptide (GRGDSP) inhibited IL-8-mediated neutrophil chemotaxis; however, these RGD peptides did not inhibit neutrophil chemotaxis in response to other chemotactic agents. Furthermore, FN fragments not containing the RGD sequence had no effect on IL-8-mediated chemotaxis. These data suggest that directed migration of neutrophils in response to IL-8 is inhibited in the presence of cell-binding fragments of FN and may represent a local mechanism for terminating neutrophil migration at areas of tissue injury.
authors.affiliation: Department of Physiology and Cell Biology, Albany Medical College, New York 12208.
authors.forename: P W
authors.initials: PW
authors.lastname: Gudewicz
authors.forename: L A
authors.initials: LA
authors.lastname: Heinel
authors.forename: K
authors.initials: K
authors.lastname: Stanton
authors.forename: D
authors.initials: D
authors.lastname: Singleton
authors.forename: M B
authors.initials: MB
authors.lastname: Frewin
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005353:Fibronectins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000166:cytology*; D009842:Oligopeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D005353:Fibronectins; D016209:Interleukin-8; D009842:Oligopeptides; D010446:Peptide Fragments; D011994:Recombinant Proteins; C047981:arginyl-glycyl-aspartic acid