title: Macaca fascicularis as a model in which to assess the safety and efficacy of a vaccine for periodontitis.
abstract: We have assessed Macaca fascicularis as a potential model in which to test the efficacy and safety of a vaccine for periodontitis. Twenty-eight animals were surveyed and 20 studied in more detail. Clinical periodontal status was assessed, the subgingival microflora analyzed especially for the presence and proportions of Porphyromonas gingivalis and titers and avidities of serum antibodies reactive with P. gingivalis measured. Probing depths ranged from 0.90 mm to 3.80 mm, Gingival Index scores from 0.00 to 4.00 and Plaque Index scores from 0.00 to 3.00. About 40% of sites bled on probing. The animals manifested a subgingival flora characteristic of the anaerobic gram-negative bacteria found in human periodontal pockets, including Actinobacillus actinomycetemcomitans, P. gingivalis, Bacteroides forsythus, Campylobacter rectus, Prevotella intermedia and Fusobacterium nucleatum. P. gingivalis was detected in 70 of 80 samples studied, ranging from 0.01% to 20% of the total flora. Serum antibody reactive with antigens of P. gingivalis was observed in all animals, with titers ranging from 1.0 enzyme-linked immunosorbent assay (ELISA) unit to 25 ELISA units and avidities from 0.10 M to 2.20 M. Antibody titer and maximum percentage of P. gingivalis were inversely correlated, indicating that a humoral immune response may be effective in reducing P. gingivalis overgrowth. M. fascicularis appears to be an excellent model for use in vaccine development.
authors.affiliation: Department of Periodontics, Regional Primate Research Center, Health Science Center, University of Washington, Seattle.
authors.forename: G R
authors.initials: GR
authors.lastname: Persson
authors.forename: L D
authors.initials: LD
authors.lastname: Engel
authors.forename: C W
authors.initials: CW
authors.lastname: Whitney
authors.forename: A
authors.initials: A
authors.lastname: Weinberg
authors.forename: B J
authors.initials: BJ
authors.lastname: Moncla
authors.forename: R P
authors.initials: RP
authors.lastname: Darveau
authors.forename: L
authors.initials: L
authors.lastname: Houston
authors.forename: P
authors.initials: P
authors.lastname: Braham
authors.forename: R C
authors.initials: RC
authors.lastname: Page
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D003773:Dental Plaque / Q000382:microbiology; D003774:Dental Plaque Index; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005069:Evaluation Studies as Topic; D005260:Female; D006089:Gram-Negative Anaerobic Bacteria / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000097:blood; D008252:Macaca fascicularis; D008297:Male; D010512:Periodontal Index; D010518:Periodontitis / Q000382:microbiology / Q000517:prevention & control*; D016966:Porphyromonas gingivalis / Q000276:immunology / Q000302:isolation & purification; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G