Chiral separations by capillary electrophoresis: present state of the art.
Capillary electrophoresis is a powerful tool for chiral separation of ionogenic enantiomers in solutions. This article brings an overview of the theory of electrophoretic separations with special emphasis on enantiomer-chiral selector equilibria, followed by a survey of indirect separations, based on formation of diastereomers with different electrophoretic mobilities, and a comprehensive appraisal of direct separations when the chiral recognition stems from (i) host-guest interactions using cyclodextrins and crown ethers as hosting agents, (ii) ligand exchange mechanism, (iii) affinity interactions or (iv) a combination of solute-chiral selector interactions with micellar electrophoretic transport. Finally, some trends in chiral separations by capillary electrophoresis are discussed.
Vespalec
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R
Institute of Analytical Chemistry, Academy of Sciences of Czech Republic, Brno.
Bocek
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P
D003505:Cyclodextrins / Q000302:isolation & purification; D004586:Electrophoresis / Q000379:methods*; D004988:Ethers, Cyclic / Q000302:isolation & purification; D007700:Kinetics; D008024:Ligands; D011134:Polysaccharides / Q000302:isolation & purification; D011485:Protein Binding; D013237:Stereoisomerism
D003505:Cyclodextrins; D004988:Ethers, Cyclic; D008024:Ligands; D011134:Polysaccharides