Enhancement of beta-galactosidase productivity of Aspergillus niger NCIM-616.
A series of mutations was carried out with Aspergillus niger NCIM-616 as the parent strain. A mutant strain NG-4 with 28% increased beta-galactosidase productivity was produced with N-methyl-N-nitro-N-nitrosoguanidine (NTG) at 1500 micrograms ml-1 concentration and exposure time of 60 min. This mutant yielded a third generation u.v.-treated strain, UV-5 with a 117.6% increase in beta-galactosidase productivity with respect to the parent strain.
Rasouli
I
I
Department of Chemical Technology, University of Bombay, India.
Kulkarni
P R
PR
D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D005524:Food Technology; D006868:Hydrolysis; D007785:Lactose / Q000378:metabolism; D008769:Methylnitronitrosoguanidine; D009154:Mutation; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
D008769:Methylnitronitrosoguanidine; D001616:beta-Galactosidase; D007785:Lactose