title: Transformation of Mollicutes with single-stranded Tn4001 DNA.
abstract: Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log lower frequency than did A. oculi ISM1499. Restriction enzyme digestion of single-stranded DNA indicated homologous base pairing in the inverted repeat regions, which could account for the transpositional activity of single-stranded DNA.
authors.affiliation: Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011.
authors.forename: P A
authors.initials: PA
authors.lastname: Kapke
authors.forename: K L
authors.initials: KL
authors.lastname: Knudtson
authors.forename: F C
authors.initials: FC
authors.lastname: Minion
mesh_terms: D000127:Acholeplasma / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics*; D008957:Models, Genetic; D009174:Mycoplasma / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded