Determination of the calcium antagonist SIM6080 in plasma by high-resolution gas chromatography.
A sensitive and selective gas chromatographic method for the determination of the calcium antagonist SIM6080 in plasma has been developed and validated. A three-step extraction procedure is employed followed by capillary gas chromatographic analysis using nitrogen-selective detection and the programmed temperature vaporizer injection technique. The defluorinated analogue was used as the internal standard. The analysis of spiked plasma demonstrated the good accuracy and precision of the method with limit of detection of 1 ng/ml. The method has been used for pharmacokinetic studies in laboratory animals.
Castelnovo
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Drug Discovery Analysis Group, Zambon Research s.p.a., Bresso-Milan, Italy.
D000042:Absorption; D000818:Animals; D002121:Calcium Channel Blockers / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D004285:Dogs; D004355:Drug Stability; D005029:Ethylenediamines / Q000097:blood* / Q000493:pharmacokinetics; D005615:Freezing; D005898:Glass; D006801:Humans; D011786:Quality Control; D012680:Sensitivity and Specificity
D002121:Calcium Channel Blockers; D005029:Ethylenediamines; C063868:SIM 6080