Expression of cytochrome P-450 3A in HT29-MTX cells and Caco-2 clone TC7.
HT-29 sublines and Caco-2 clones were analyzed for the expression of cytochrome P-450 3A. The enzyme was found to be expressed in differentiated HT-29 cells selected by resistance to methotrexate and in one of seven Caco-2 clones, TC7. Its expression parallels the differentiation process, with highest levels being observed at late confluency. P-450 3A mRNA and protein patterns, as well as subcellular distribution, are intermediate between those observed in human adult intestine and fetal liver.
Carrière
V
V
Unité de Recherches de Biochimie Pharmacologique et Métabolique (INSERM U75), Centre Hospitalo-Universitaire Necker-Enfants Malades, Paris, France.
Lesuffleur
T
T
Barbat
A
A
Rousset
M
M
Dussaulx
E
E
Costet
P
P
de Waziers
I
I
Beaune
P
P
Zweibaum
A
A
D015152:Blotting, Northern; D015153:Blotting, Western; D002277:Carcinoma / Q000201:enzymology*; D002451:Cell Compartmentation; D002454:Cell Differentiation; D002999:Clone Cells; D003110:Colonic Neoplasms / Q000201:enzymology*; D019392:Cytochrome P-450 CYP2E1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012641:Selection, Genetic; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D019392:Cytochrome P-450 CYP2E1; D008727:Methotrexate