title: Group-selective immunoassay.
abstract: A new solid-phase immunoassay technique has been applied for anti-digoxin monoclonal antibodies (Mabs) detection. At the first assay step, anti-digoxin Mabs (IgG1, Kaff = 9.2 x 10(9) M-1) were bound to a specially prepared immunosorbent, the microtiter plates coated with digoxin-human serum albumin conjugate (Dig-HSA), in which free amino groups were protected by a glutaraldehyde cross-linking modification. The modification did not essentially influence the antibody-binding capacity of the immunosorbent. After antigen-antibody reaction, free amino groups were located only on the anti-digoxin Mabs, bound to chemically modified immunosorbent. At the second assay step, free amino groups of anti-digoxin Mabs were biotinylated by N-hydroxysuccinimide-biotin ester. Then the biotin residues were detected by the streptavidin-peroxidase conjugate. The method does not require second labeled antibodies and may be used for anti-hapten hybridoma screening.
authors.affiliation: Department of Molecular Genetic Immunology, V.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
authors.forename: S A
authors.initials: SA
authors.lastname: Yazynin
authors.forename: S M
authors.initials: SM
authors.lastname: Deyev
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D004077:Digoxin / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007124:Immunoenzyme Techniques
chemical_list: D000911:Antibodies, Monoclonal; D004077:Digoxin