Effects of flavonoid compounds on the activity of NADPH diaphorase prepared from the mouse brain.
The effects of flavonoids on NADPH diaphorase activity were studied in vitro, and we found that the enzyme activity was markedly inhibited by quercetin. This inhibitory action was shown to be accompanied by an increase in the apparent Km value of the enzyme for the cofactor NADPH, with a decrease in the Vmax, and an increase in the apparent Km for the substrate nitro blue tetrazolium, without any significant change in the Vmax. These results indicate that quercetin may directly inhibit NADPH diaphorase, thus suggesting the possibility that this compound may be able to inhibit the production of nitric oxide in the brain.
Tamura
M
M
Department of Urology, Tokushima University School of Medicine, Japan.
Kagawa
S
S
Tsuruo
Y
Y
Ishimura
K
K
Morita
K
K
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D020843:Chamomile; D047309:Flavones; D005419:Flavonoids / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009252:NADPH Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009822:Oils, Volatile / Q000494:pharmacology; D010946:Plants, Medicinal; D011794:Quercetin / Q000494:pharmacology
D047309:Flavones; D005419:Flavonoids; D009822:Oils, Volatile; D011794:Quercetin; D009252:NADPH Dehydrogenase; C043562:flavone