The influence of picolines on glutathione transferase activity and subunit composition in human liver derived Hep G2 cells.
Hep G2 cells, an established cell line derived from a human hepatoma, have retained a number of hepatocytic phase I and II reactions. The influence of picolines (2-, 3- and 4-methylpyridine), related compounds and some classical enzyme inducers on specific glutathione transferase (GST) activity and its subunit composition in Hep G2 cells was investigated. Increased GST activity was observed for rifamycin, phenobarbital, pyrazine and the picolines, of which the 4-isomer was the strongest inducer. The GST subunits were analysed by HPLC. GSTP1, GSTM1a, GSTA1 and GSTA2 were present in control Hep G2 cells. GSTM1a disappeared or was strongly reduced under the influence of the test chemicals. All GST increases were due to augmented GSTA1 expression. Thus, picolines stimulate GST activity in Hep G2 cells by influencing the class alpha GSTA1.
Dierickx
P J
PJ
Instituut voor Hygiëne en Epidemiologie, Afdeling Toxikologie, Brussel, Belgium.
D004790:Enzyme Induction; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D010847:Picolines / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D010847:Picolines; D005982:Glutathione Transferase