Lack of promoting effect of proline on bile duct cancer development in dimethylnitrosamine-initiated hamster livers.
Bile duct hyperplasia caused by proline is believed to represent a chemical effect of the liver fluke, Fasciola hepatica, and the resultant cell division might be expected to play a role as a tumor promoter. To investigate the potential promoting effect of proline on bile duct cancer development, Syrian hamsters were therefore divided into 8 treatment groups: dimethylnitrosamine (DMN) + proline intraperitoneally (i.p.); DMN + proline s.c.; DMN + saline i.p.; DMN + saline s.c.; proline i.p.; proline s.c.; saline i.p.; and saline s.c. DMN was injected i.p. at 20 mg/kg to the animals 2 weeks prior to commencement of proline treatment, whereby 1 ml of a 2 M solution was given by i.p. or s.c. injection 3 times a week for 20 weeks. At the end of week 42, assessment of preneoplastic lesion development did not reveal any significant modulating influence of proline on DMN-initiated lesion development nor did it itself cause persistent bile duct hyperplasia.
Thamavit
W
W
Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok.
Pairojkul
C
C
Tiwawech
D
D
Shirai
T
T
Ito
N
N
D000818:Animals; D001650:Bile Duct Neoplasms / Q000139:chemically induced*; D001652:Bile Ducts / Q000187:drug effects / Q000473:pathology; D006224:Cricetinae; D004128:Dimethylnitrosamine; D006965:Hyperplasia; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced*; D008297:Male; D008647:Mesocricetus; D011230:Precancerous Conditions / Q000139:chemically induced*; D011392:Proline / Q000633:toxicity*
D011392:Proline; D004128:Dimethylnitrosamine