Methoxypsoralen phototherapy of transitional cell carcinoma.
OBJECTIVE
The goal of this research was to assess whether methoxypsoralen compounds in combination with ultraviolet light were effective in preventing cellular proliferation in an in vitro model of human transitional cell carcinoma.


METHODS
Three methoxypsoralen compounds, 5-methoxypsoralen (5-MOP), 8-methoxypsoralen (8-MOP), and 4'-aminomethyl 4,5'-8'-trimethylpsoralen (AMT), were added in vitro to T-24 transitional cell carcinoma cells. Psoralens directly bind to DNA, cross-linking the strands when exposed to ultraviolet light and thereby prevent cellular division.


RESULTS
In vitro activity was demonstrated utilizing AMT and ultraviolet radiation at 320 to 340 nm, preventing cellular proliferation in T-24 transitional cell carcinoma.


CONCLUSIONS
Methoxypsoralen compounds in combination with ultraviolet light are effective in preventing proliferation of bladder carcinoma cells in vitro. This therapy may prove to be effective in clinical early stage transitional cell carcinoma and warrants further assessment.
Keane
T E
TE
Department of Surgery, Emory University School of Medicine, Atlanta, Georgia.
Petros
J A
JA
Velimirovich
B
B
Yue
K T
KT
Graham
S D
SD
D000078223:5-Methoxypsoralen; D002295:Carcinoma, Transitional Cell / Q000473:pathology*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008730:Methoxsalen / Q000031:analogs & derivatives / Q000494:pharmacology*; D014307:Trioxsalen / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D014466:Ultraviolet Rays; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
D000078223:5-Methoxypsoralen; D008730:Methoxsalen; D014307:Trioxsalen