title: Penetration of a dentin bonding agent into dentin.
abstract: OBJECTIVE
  To examine how the priming agents of Scotchbond Multi-Purpose (SMP) influenced the wettability of collagen in the demineralized zone and hence the penetration and adaptation of its bonding monomers.
  
  
  METHODS
  Freshly extracted bovine teeth were ground to dentin with SiC paper. SMP and Z100 resin were used. Shear bond testing was performed as well as scanning electron microscopic evaluation.
  
  
  RESULTS
  In contrast to the manufacturer's instructions, a 26.3% increase in shear bond strength was obtained when a second coat of priming agent was applied. This increase was statistically significant at the 5% level of confidence. SEM examination of failed conventionally air dried (CAD) specimens showed the presence of voids in the demineralized layer. SEM examination of failed specimens prepared by a critical point drying (CPD) technique showed that these voids were filled with collagen fibrils. The CPD technique also clearly showed that collagen fibrils were incorporated in the unfilled resin. Although priming agents appear to facilitate the penetration of unfilled resin into the demineralized layer and the dentin tubules, areas of unincorporated collagen fibrils can be seen even after two or more applications of the priming agent.
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dentistry, University of Toronto, Ontario, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Titley
authors.forename: R
authors.initials: R
authors.lastname: Chernecky
authors.forename: B
authors.initials: B
authors.lastname: Maric
authors.forename: D
authors.initials: D
authors.lastname: Smith
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry; D001840:Dental Bonding; D003793:Dental Restoration, Permanent / Q000379:methods; D003804:Dentin / Q000648:ultrastructure; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D019279:Resin Cements; D017155:Wettability
chemical_list: D017220:Dentin-Bonding Agents; D019279:Resin Cements; C084018:Scotchbond Multi-Purpose; D003094:Collagen