Cloning and characterization of a sulphite-resistance gene of Saccharomyces cerevisiae.
In this paper we describe the cloning and sequencing of the gene (SUL1) responsible for sulphite resistance in a Saccharomyces cerevisiae mutant (Casalone et al., 1992). The deduced amino acid sequence predicted that the gene codes for a zinc-finger protein with five fingers. Comparison of wild-type and mutant gene sequences demonstrated that the mutation event was a transversion from C to G; as a consequence of the mutation a histidine substituted an aspartic acid, affecting directly the fourth finger structure. The SUL1 gene sequence corresponds to that of FZF1 gene (Breitwieser et al., 1993) to which no function was attributed.
Casalone
E
E
Department of Animal Biology and Genetics, University of Florence, Italy.
Colella
C M
CM
Daly
S
S
Fontana
S
S
Torricelli
I
I
Polsinelli
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D018628:Gene Dosage; D005800:Genes, Fungal / Q000235:genetics*; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D017354:Point Mutation; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013447:Sulfites / Q000494:pharmacology*; D014157:Transcription Factors; D016335:Zinc Fingers
C090870:FZF1 protein, S cerevisiae; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D013447:Sulfites; D014157:Transcription Factors