[Methodological study on the determination of oxiracetam concentration in serum and urine by HPLC].
A method was developed for the determination of oxiracetam concentration in serum and urine by HPLC. Acyclovir was used as an internal standard. The analytical column was a stainless-steel column (30 mm x 4.6 mm ID) filled with 10 microns Bondapak NH2. A mixture of acetonitrile and water (80: 20) as mobile phase was used at a flow rate of 1 ml.min-1. Detection was performed at 210 nm. The retention times were 6.3 min for oxiracetam and 8.1 min for the internal standard. The lower detection limits were 1 microgram.ml-1 for serum and 20 micrograms.ml-1 for urine. The precision and accuracy within-day and day-to-day for both serum and urine samples ranged from 5.0 to 10.7%. The mean recoveries were 99.7 +/- 5.9% and 99.0 +/- 5.6% for human serum and urine, respectively. The results showed that the method is simple, rapid, sensitive, reliable and good enough to be used in studying the clinical pharmacokinetics of oxiracetam.
Jiao
X L
XL
Department of Pharmacology, Beijing Medical University.
Yu
D H
DH
Zou
A Q
AQ
Lou
Y Q
YQ
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D011759:Pyrrolidines / Q000032:analysis* / Q000493:pharmacokinetics
D011759:Pyrrolidines; C040619:oxiracetam