Mixtures of erythrocytes and acellular fluids: an in vitro evaluation.
To evaluate the biophysical properties of two acellular resuscitative fluids, experiments were performed using mixtures of these with suspensions of red blood cells. Two acellular resuscitative fluids were synthesized using a diacid to tetramerically stabilized hemoglobin and then complexing it with either a hydroxyethyl starch or a Tetronic Polyol. The new polymers were characterized with respect to the molecular weight, second virial coefficient and intrinsic viscosity. These fluids were then combined with red cells and the following measurements were made: non-Newtonian flow properties, malonamide induced hemolytic kinetics, sedimentation rates and oxygen transport. The in vitro evaluation indicates that these compounds are effective hemodiluents, offer protection to the red cell membrane and do not cause erythrocyte aggregation. The oxygen transport was satisfactory.
Cerny
L C
LC
Department of Chemistry, Utica College of Syracuse University, NY 13502.
Cerny
E R
ER
Barnes
B
B
Ferreira
A
A
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D001799:Blood Sedimentation; D001802:Blood Substitutes / Q000737:chemistry* / Q000378:metabolism; D004912:Erythrocytes / Q000502:physiology*; D005078:Exchange Transfusion, Whole Blood; D006438:Hemodilution; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D014783:Viscosity
D001802:Blood Substitutes; D006454:Hemoglobins; C037158:polyhemoglobin; D010100:Oxygen