title: Cytotoxin-converting phages, phi CTX and PS21, are R pyocin-related phages.
abstract: phi CTX is a temperate phage of Pseudomonas aeruginosa harbouring the ctx gene that encodes cytotoxin (CTX). We identified phi CTX as an R pyocin-related phage, by serological and molecular analysis, based on the findings that the infectivity of the phage was inhibited with the antisera directed R pyocins and R pyocin-related phages and that the phi CTX genome showed DNA homology to the genome of PS17 (a representative of the R pyocin-related phages) as well as to the pyocin R2 genes. Another new CTX-converting, R pyocin-related phage named PS21 was isolated from a CTX-producing strain of P. aeruginosa, suggesting the distribution of the ctx gene by certain members of R pyocin-related phage family.
authors.affiliation: Department of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: M
authors.initials: M
authors.lastname: Ohnishi
authors.forename: S
authors.initials: S
authors.lastname: Yokota
authors.forename: T
authors.initials: T
authors.lastname: Shinomiya
authors.forename: M
authors.initials: M
authors.lastname: Kageyama
authors.forename: Y
authors.initials: Y
authors.lastname: Terawaki
mesh_terms: D036002:ADP Ribose Transferases; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics*; D005098:Exotoxins / Q000096:biosynthesis / Q000235:genetics*; D016679:Genome, Viral; D017105:Pseudomonas Phages / Q000145:classification* / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000821:virology*; D011709:Pyocins; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D011709:Pyocins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A