title: Hypericin uptake in rabbits and nude mice transplanted with human squamous cell carcinomas: study of a new sensitizer for laser phototherapy.
abstract: Tissue uptake and biodistribution of hypericin was measured in rabbits and in nu/nu mice xenografted with P3 human squamous cell carcinoma to assess the value of this dye as an in vivo sensitizer for laser photoinactivation of solid tumors. Hypericin has absorption maxima at 545 and 590 nm with a fluorescence emission peak at 640 nm in ethanol. Dye uptake after intravenous injection was tested at 4 and 24 hours in rabbit tissues by ethanol extraction and quantitative fluorescence spectrophotometry. Maximum dye levels were seen at 4 hours in most vascular organs with lung having fivefold higher uptake than spleen followed by liver, blood, and kidney. Mice were examined after 2, 4, 6, 8, and 24 hours and after 3 and 7 days for dye uptake. The peak concentration of hypericin in murine organs was reached at 4 hours with uptake per gram of tissue as follows: lung > spleen > liver > blood > kidney > heart > gut > tumor > stomach > skin > muscle > brain. Elimination of hypericin was rapid in most murine organs with residual dye under 10% of maximum by 7 days compared to 25% to 30% retention for the squamous cell tumors and several normal tissues. These results suggest that hypericin may be a useful photosensitizer for KTP/532 laser interstitial therapy of human cancer.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Dankook University, College of Medicine, Cheonan, Korea.
authors.forename: P S
authors.initials: PS
authors.lastname: Chung
authors.forename: R E
authors.initials: RE
authors.lastname: Saxton
authors.forename: M B
authors.initials: MB
authors.lastname: Paiva
authors.forename: C K
authors.initials: CK
authors.lastname: Rhee
authors.forename: J
authors.initials: J
authors.lastname: Soudant
authors.forename: A
authors.initials: A
authors.lastname: Mathey
authors.forename: C
authors.initials: C
authors.lastname: Foote
authors.forename: D J
authors.initials: DJ
authors.lastname: Castro
mesh_terms: D000818:Animals; D000873:Anthracenes; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010569:Perylene / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D010778:Photochemotherapy / Q000379:methods*; D011817:Rabbits; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics*; D013154:Spleen / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D000873:Anthracenes; D011838:Radiation-Sensitizing Agents; D010569:Perylene; C004965:hypericin