title: Calcium-induced translocation of annexins to subcellular organelles of human neutrophils.
abstract: The annexins are Ca(2+)-regulated, phospholipid-binding proteins which have been suggested to take part in cellular events such as exocytosis. The subcellular localization of annexins in human neutrophils was determined using monoclonal antibodies against annexins I, II, IV and VI and a polyclonal peptide antiserum against an annexin consensus sequence. Several annexins were translocated to the light membrane fraction enriched in plasma membranes and secretory vesicles. Annexins were associated also with the azurophil and specific granules. Whereas annexins I, IV and VI and one unidentified 35 kDa protein translocated to each of the isolated organelles, annexin II, a 66 kDa annexin IV-like protein, and a 38 kDa annexin I-like protein exhibited organelle-related differences in their association with membranes. The 38 kDa annexin associated only with specific granules and the secretory vesicles/plasma membrane but not with azurophil granules. Annexin II and the 66 kDa annexin IV-like protein associated with each of the neutrophil organelles, but the binding to specific granules and secretory vesicles/plasma membrane showed a Ca(2+)-dependency different from that of azurophil granules. This observation suggests that these proteins may contribute to the secretory process in neutrophils.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Sjölin
authors.forename: O
authors.initials: O
authors.lastname: Stendahl
authors.forename: C
authors.initials: C
authors.lastname: Dahlgren
mesh_terms: D000595:Amino Acid Sequence; D017302:Annexins / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism* / Q000648:ultrastructure; D015388:Organelles / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D017302:Annexins; D000911:Antibodies, Monoclonal; D002118:Calcium