Antigenic analysis of Dictyocaulus viviparus by use of test-positive bovine sera.
An antigen extract of Dictyocaulus viviparus was analyzed by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the antigen-recognition patterns of serum antibody from cattle not infected, cattle infected with D viviparus, and cattle with unknown history of D viviparus were analyzed by the use of ELISA and western blotting techniques. Cross-reactive antibody-recognition patterns were determined by comparing western blots of D viviparus-positive sera with blots of D viviparus-negative sera obtained from cattle singly infected with Bunostomum phlebotomum, Cooperia oncophora, C punctata, Nematodirus helvetianus, Oesophagostomum radiatum, or Ostertagia ostertagi. Five antigen bands unique to D viviparus were identified, and their frequency of appearance in western blots of sera from verified D viviparus-positive and -negative cattle, and sera from cattle exposed to the parasite, but with unknown D viviparus immune status, were determined. Of the 5 antigens unique to D viviparus, 29- and 19-kd bands had the highest frequencies of reaction (45.9 and 59.0%, respectively) with the test sera. These bands had strong reactivity with sera containing antibodies to D viviparus and did not react with the heterologous sera. We conclude that the 29- and 19-kd antigens may be useful for developing an improved serodiagnostic test for D viviparus infections in cattle.
Hale
G L
GL
Department of Veterinary Microbiology, College of Veterinary Medicine, University of Missouri, Columbia 65211.
Green
T J
TJ
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004022:Dictyocaulus Infections / Q000175:diagnosis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D012698:Serologic Tests
D000947:Antigens, Helminth