A rapid and efficient PCR-based method for synthesizing high-molecular-weight multimers of oligonucleotides.
We have developed a simple PCR based approach for synthesizing high-molecular-weight DNA multimers of short oligonucleotide elements in yields sufficient for preparing DNA affinity columns or for using as probes to detect sequence-specific DNA binding proteins. The technique is rapid, efficient and produces multimers consisting of 30-100 repeating units.
Hemat
F
F
Laboratory of Structural Biology and Molecular Medicine, UCLA School of Medicine 90024.
McEntee
K
K
D001483:Base Sequence; D001704:Biopolymers; D002846:Chromatography, Affinity; D004247:DNA / Q000138:chemical synthesis*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction / Q000379:methods*
D001704:Biopolymers; D004247:DNA