Gas chromatographic-mass spectrometric determination of ibuprofen enantiomers in human plasma using R(-)-2,2,2-trifluoro-1-(9-anthryl)ethanol as derivatizing reagent.
A relatively rapid, inexpensive, sensitive and stereospecific gas chromatographic-mass spectrometric method was developed for the quantification of S(+) and R(-)-ibuprofen in human plasma. This method uses a commercially available internal standard and has no interference from endogenous substances nor metabolites. The method involves derivatization of ibuprofen enantiomers with optically active R(-)-2,2,2-trifluoro-1-(9-anthryl)ethanol using oxalyl chloride as the coupling reagent. The subsequently formed diastereoisomers are separated by gas chromatography and analysed by mass spectrometry using selected-ion monitoring. The assay is successfully applied to a pharmacokinetic study. The simplicity, sensitivity and precision of the method make it convenient for the quantification of ibuprofen enantiomers in biological samples.
Zhao
M J
MJ
Laboratoire de Chimie Thérapeutique, Faculté de Pharmacie, Illkirch, France.
Peter
C
C
Holtz
M C
MC
Hugenell
N
N
Koffel
J C
JC
Jung
L
L
D000873:Anthracenes; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007052:Ibuprofen / Q000097:blood* / Q000493:pharmacokinetics; D007202:Indicators and Reagents; D013237:Stereoisomerism; D014270:Trifluoroethanol / Q000031:analogs & derivatives
D000873:Anthracenes; D007202:Indicators and Reagents; C090817:2,2,2-trifluoro-1-(9-anthryl)ethanol; D014270:Trifluoroethanol; D007052:Ibuprofen