Optimized conditions for the fermentation of Helicobacter pylori and production of vacuolating cytotoxin.
We report here improvements to the growth media and fermentation conditions which result in a substantial increase of Helicobacter pylori growth and in the enhanced production of vacuolating cytotoxin. Addition of glucose to the medium resulted in the increase of cell yield, cell viability and a significant improvement in the production of vacuolating cytotoxin.
Marchini
A
A
IRIS, Biocine Immunobiological Research Institute Siena, Italy.
Massari
P
P
Manetti
R
R
Olivieri
R
R
D001426:Bacterial Proteins / Q000096:biosynthesis*; D001427:Bacterial Toxins / Q000096:biosynthesis; D018533:Biomass; D003470:Culture Media; D003603:Cytotoxins / Q000096:biosynthesis*; D005285:Fermentation; D005947:Glucose; D016480:Helicobacter pylori / Q000254:growth & development / Q000378:metabolism*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; D003603:Cytotoxins; C074689:VacA protein, Helicobacter pylori; D005947:Glucose