title: The ecdysone response enhancer of the Fbp1 gene of Drosophila melanogaster is a direct target for the EcR/USP nuclear receptor.
abstract: The transcription of the Drosophila melanogaster Fbp1 gene is induced by the steroid hormone 20-hydroxyecdysone and restricted to the late-third-instar fat body tissue. In a previous study we showed that the -68 to -138 region relative to the transcription start site acts as an ecdysone-dependent third-instar fat body-specific enhancer in a transgenic assay. Here we report that seven nucleoprotein complexes are formed in vitro on this enhancer when a nuclear extract from late-third-instar fat body is used in a gel shift assay. Accurate mapping of the binding sites of the complexes revealed a remarkably symmetrical organization. Using specific antibodies, one of the complexes was identified as a heterodimer consisting of the ecdysone receptor (EcR) and Ultraspiracle (USP) proteins. The binding site of the heterodimer as defined by mutagenesis and methylation interference experiments bears strong sequence similarity to the canonical hsp27 ecdysone response element, including an imperfect palindromic structure. The two elements diverge at three positions in both half-sites, indicating that the structure of an active EcR/USP binding site allows considerable sequence variations. In vivo footprinting experiments using ligation-mediated PCR and wild-type or ecdysteroid-deficient larvae show that occupancy of the Fbp1 EcR/USP binding site and adjacent region is dependent on a high concentration of ecdysteroids. These results provide strong evidence for a direct role of the EcR/USP heterodimer in driving gene expression in response to changes of the ecdysteroid titer during Drosophila larval development.
authors.affiliation: Institut Jacques Monod, Centre National de la Recherche Scientifique, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Antoniewski
authors.forename: M
authors.initials: M
authors.lastname: Laval
authors.forename: A
authors.initials: A
authors.lastname: Dahan
authors.forename: J A
authors.initials: JA
authors.lastname: Lepesant
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D017931:DNA Primers; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D004440:Ecdysone / Q000502:physiology*; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000378:metabolism; D007814:Larva; D008745:Methylation; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C087777:Fbp1protein, Drosophila; D006360:Heat-Shock Proteins; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D004440:Ecdysone