title: Improved purification of arylsulfate sulfotransferase from human intestinal bacterium by using polyclonal antibody.
abstract: Arylsulfate sulfotransferase (ASST) from a human intestinal bacterium stoichiometrically catalyzed the transfer of the sulfate group of phenylsulfate esters to phenolic compounds. Polyclonal antibodies against ASST were obtained from rabbit sera. These antisera did not inhibit ASST activity. ASST was recognized by the IgG fraction of the antisera, but rat liver phenol sulfotransferase did not show cross-reactivity to ASST on Western blot (immunoblot) analysis. The ASST was purified by an anti-ASST immobilized affinity column chromatography to homogeneity on SDS-PAGE. The NH2-terminal amino acid and partial sequence of the purified enzyme were serine and SVKYSFEDHIINRQYEAEQAMLAKF, respectively. We corrected the previous result that the NH2-terminal of ASST was arginine.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University.
authors.forename: L
authors.initials: L
authors.lastname: Konishi-Imamura
authors.forename: K
authors.initials: K
authors.lastname: Dohi
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Kobashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015239:Arylsulfotransferase / Q000032:analysis / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D005051:Eubacterium / Q000201:enzymology*; D006801:Humans; D015151:Immunoblotting; D007422:Intestines / Q000382:microbiology*; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats
chemical_list: D000906:Antibodies; D015239:Arylsulfotransferase; C090707:arylsulfate sulfotransferase