In vitro activation of the classical pathway of complement by a streptococcal lipoteichoic acid.
The purpose of this study was to find whether a glycerolphosphate-containing lipoteichoic acid prepared from Streptococcus sobrinus OMZ 176 cells would activate the classical pathway of complement while in solution. Reference activators were lipopolysaccharide from Escherichia coli 0111:B4 and heat-aggregated immunoglobulin G. Serum samples were taken from healthy students. Analysis through crossed immunoelectrophoresis showed that lipoteichoic acid caused an almost complete dissociation of the C1qrs macromolecule. All activators decreased the area of and slowed the electrophoretic mobility of the C4 protein peaks, with lipoteichoic acid causing the most pronounced alterations. Electroimmunoassays showed that lipoteichoic acid separately, yielded detectable amounts of free C1r2s2 subunits; it also generated significantly more trimer complexes between C1r, C1s and C1 inhibitor (C1INH) than did the other two activators. Lipoteichoic acid was, however, a comparatively weak inducer of tetramer C1INH-C1r-C1s-C1INH complexes. Analysis through Western blotting showed that all activators accelerated consumption of C1r, induced complex formations between C1INH and C1s and produced cleavage products of C2. Altogether, the immunochemical analysis gave clear evidence of classical pathway activation by lipoteichoic acid, but its activation profile differed from those seen with lipopolysaccharide and aggregated immunoglobulin G.
Monefeldt
K
K
Department of Periodontology, Dental Faculty, University of Oslo, Norway.
Helgeland
K
K
Tollefsen
T
T
D000704:Analysis of Variance; D015153:Blotting, Western; D003172:Complement C1; D015923:Complement C1r; D003173:Complement C1s; D003175:Complement C2; D003181:Complement C4; D003171:Complement Pathway, Classical / Q000276:immunology*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008070:Lipopolysaccharides / Q000276:immunology*; D016986:Streptococcus sobrinus / Q000276:immunology*; D013682:Teichoic Acids / Q000276:immunology*
D003172:Complement C1; D003175:Complement C2; D003181:Complement C4; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid; D015923:Complement C1r; D003173:Complement C1s