[Effects of bimolane on cell cycle cytokinesis in human lymphocytes in vitro].
Bimolane, 1,2-bis(4-morpholinomethyl-3,5-dioxopiperazinyl) ethane, is a new antineoplastic agent synthesized first in China. The effects of bimolane on the rate of S phase cells, mitotic index, and cytokinesis in human peripheral blood lymphocytes cultured in vitro were studied by autoradiography with [3H]TdR and counting the number of mono- and multi-nucleated lymphocytes. Results showed that bimolane (5, 10, 20 micrograms.ml-1) inhibited the progression of cell cycle, so that the rate of S and M phases decreased. Bimolane inhibited cytokinesis, which formed bi- and multi-nucleated cells. The effects were concentration-dependent. Bimolane induced micronuclei in mono- and bi-nucleated lymphocytes. Our results indicate that bimolane is a cytotoxic and genotoxic agent.
Xue
K X
KX
Jiangsu Institute of Cancer Research, Nanjing, China.
Ma
G J
GJ
Wu
J Z
JZ
D000293:Adolescent; D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D008938:Mitosis / Q000187:drug effects; D011929:Razoxane / Q000031:analogs & derivatives* / Q000494:pharmacology
D000970:Antineoplastic Agents; C031186:bimolane; D011929:Razoxane