High-performance liquid chromatography with UV detection and diode-array UV confirmation of isonicotinic acid hydrazide in cattle milk.
A method for the determination of isonicotinic acid hydrazide (isoniazid) in milk was developed. Milk was deproteinized with trichloroacetic acid. Isoniazid was condensed with cinnamaldehyde and assayed on a reversed-phase HPLC system, with good sensitivity and accuracy (10 micrograms/l) with UV detection at 330 nm. Use of solid-phase extraction with a C18 cartridge allows the detection limit to be lowered to 0.1 microgram/l with UV detection and confirmation of isoniazid hydrazone from the diode-array UV spectrum.
Defilippi
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Laboratorio di Sanità Pubblica USSL No. 40, Ivrea (TO), Italy.
Piancone
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Costa Laia
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Balla
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Tibaldi
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D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D007538:Isoniazid / Q000032:analysis* / Q000097:blood; D008892:Milk / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D007538:Isoniazid