[The use of a biotinated probe for the detection of the genomic RNA of the hepatitis A virus in clinical specimens].
A detection technique for hepatitis A virus (HAV) RNA by means of molecular hybridization using ss-biotinated DNA probe on the basis of M13 bacteriophage is described. The technique sensitivity reached 1-10 pg of control DNA or 100-500 pg of HAV. The experiments for detection of HAV carriers among the patients and contacts from foci of HAV outbreaks were carried out. A comparative analysis of the above technique and enzyme immunoassay and amplification technique was done and good coincidence of the results was demonstrated.
Budiak
E V
EV
Sinagatullina
N M
NM
Karpukhin
A V
AV
Naumov
V A
VA
Mamaeva
N V
NV
Chizhikov
V E
VE
Zolotova
V F
VF
Mertvetsov
N P
NP
D017104:Bacteriophage M13; D001710:Biotin; D002353:Carrier State / Q000097:blood; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D006506:Hepatitis A / Q000097:blood; D006507:Hepatovirus / Q000235:genetics*; D006801:Humans; D007124:Immunoenzyme Techniques; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral; D001710:Biotin
Ispol'zovanie biotinirovannogo zonda dlia detektsii genomnoĭ RNK virusa gepatita A v klinicheskikh obraztsakh.