[Role of the non-peptide thymic factor--hypoxanthine system in the proliferation of mature thymocytes of mice and rats].
The incorporation of [8-14C]hypoxanthine radioactive label into the nucleic acids of the mature thymocytes (that do not bind peanut agglutinin) of CBA mice and Wistar rats and the action of non-peptide thymic mitogenic factor (factor) on this process have been studied. It was shown, that the factor, shifting AMP catabolism towards hypoxanthine accumulation, accelerated by 50% the incorporation of hypoxanthine radioactive label into nucleic acids during 3-hour incubation. It is concluded that the factor being an activator of the hypoxanthine accumulation, abrogates the purine limits in thymocytes and activates the "salvage pathway" of purine synthesis which is the primary pathway in thymocytes. The factor is assumed to act as the mitogen on one of the stages of nucleic acids or nucleotides synthesis, but not to act on the guanine-hypoxanthine phosphoribosyl transferase. It was shown that the intrathymocyte ratio of hypoxanthine and factor concentrations (hypoxanthine/factor) is higher in rats than in mice. The dependence of proliferative activity on relative levels of both substances was revealed. The stimulation of cell proliferation by the system: factor--hypoxanthine is one of the mechanisms of thymus regeneration at the expense of mature thymocyte population.
Bezvershenko
I A
IA
Selishcheva
M Iu
MIu
Premyslov
V Kh
VKh
D000818:Animals; D002455:Cell Division / Q000502:physiology; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000737:chemistry / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D008934:Mitogens / Q000737:chemistry / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D013950:Thymus Gland / Q000166:cytology*; D013951:Thymus Hormones / Q000737:chemistry / Q000502:physiology*
D007042:Hypoxanthines; D008934:Mitogens; D013951:Thymus Hormones; D019271:Hypoxanthine
Vlyianye khymycheskoĭ modifykatsyy ostatkov arhinina fibrinohena i eho DH-frahmenta na ksorost' hidroliza étukh belkov plazminom.