Sequence patterns and hybridization analysis of clones generated by Alu-PCR.
A series of clones from an Alu-PCR library were analysed in more detail. Characterization by Southern blot hybridization and sequencing displayed several features common to all probes generated by this approach: Short length of the PCR-products as well as the presence of homologous regions on both ends resulted in a limited feasibility for filter hybridization and a low probability of restriction length polymorphisms. In addition, a series of different short repeats at the 3'-ends of Alu-repeats and present in the generated probes offers a rich source of potential variable sites accessible by PCR.
Müllenbach
R
R
Institute of Anthropology and Human Genetics, University of Tübingen, Germany.
Tisborn
S
S
Blin
N
N
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D015342:DNA Probes; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D012377:Rodentia; D017422:Sequence Analysis, DNA
D015342:DNA Probes