[Cloning transport protein genes for two strains of tobacco mosaic virus and their expression in Escherichia coli cells].
Recombinant constructs expressing fusion (His)6 movement proteins of two strains (Cruciferous and Vulgare) of tobacco mosaic virus were generated by cloning the PCR-amplified fragments into the pQE-9 vector. The movement proteins containing N-terminal (His)6 affinity tags were purified on a metal chelate adsorbent.
Ivanov
K I
KI
Ivanov
P A
PA
Timofeeva
E K
EK
Efimov
V A
VA
Dorokhov
Iu L
IuL
D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
D002352:Carrier Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins
Klonirovanie genov transportnykh belkov dvukh shtammov virusa tabachnoĭ mozaiki i ikh ékspressiia v kletkakh Escherichia coli.