title: Free radical-induced double base lesions.
abstract: Evidence is presented for the formation of products in irradiated dinucleoside monophosphates in which both bases are damaged. The dinucleoside monophosphates d(GpT), d(GpC), d(TpG) and d(CpG) were X-irradiated in oxygenated aqueous solution. Product identification was by NMR spectroscopy. In products containing double base lesions, guanine is converted to 8-hydroxyguanine and the pyrimidine base is degraded to a formamido remnant.
authors.affiliation: Biophysics Department, Roswell Park Cancer Institute, Buffalo, New York 14263.
authors.forename: H C
authors.initials: HC
authors.lastname: Box
authors.forename: H G
authors.initials: HG
authors.lastname: Freund
authors.forename: E E
authors.initials: EE
authors.lastname: Budzinski
authors.forename: J C
authors.initials: JC
authors.lastname: Wallace
authors.forename: A E
authors.initials: AE
authors.lastname: Maccubbin
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003845:Deoxycytosine Nucleotides / Q000737:chemistry / Q000528:radiation effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects; D015226:Dinucleoside Phosphates / Q000737:chemistry / Q000528:radiation effects*; D005609:Free Radicals; D006151:Guanosine / Q000031:analogs & derivatives; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation; D015394:Molecular Structure; D014965:X-Rays
chemical_list: D003845:Deoxycytosine Nucleotides; D015226:Dinucleoside Phosphates; D005609:Free Radicals; D006151:Guanosine; C037781:deoxycytidylyl-(3'-5')-deoxyguanosine; C046215:8-hydroxyguanosine; C077117:thymidylyl-(3',5')-2'-deoxyguanosine; D003849:Deoxyguanosine