An unusual temperature dependence of malondialdehyde formation in Fe2+/H2O2-initiated lipid peroxidation of phosphatidylcholine liposomes.
Determination of malondialdehyde is a widely used procedure for measurement of lipid peroxidation. In this paper we report an unusual temperature dependence of malondialdehyde formation in egg yolk phosphatidylcholine liposomes oxidized by the Fenton system (0.1 mmol/l FeSO4 and 0.05 mmol/l H2O2). The amount of malondialdehyde formed was 37% higher in samples kept at 22 degrees C than at 50 degrees C. An alternative method for determination of lipid peroxidation, measurement of oxygen uptake, revealed complete consumption of dissolved oxygen to peroxidized lipids at 22 degrees C as well as 50 degrees C. Since oxygen is essential for the formation of cyclic peroxides--precursors of malondialdehyde--we conclude that the nature of the observed effect consists in limitation of oxygen availability at elevated temperatures.
Misík
V
V
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Brastilava.
Gergel'
D
D
Alov
P
P
Ondrias
K
K
D000818:Animals; D004530:Egg Yolk; D005296:Ferrous Compounds; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008081:Liposomes / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D013696:Temperature
D005296:Ferrous Compounds; D008081:Liposomes; D010713:Phosphatidylcholines; C020748:ferrous sulfate; D008315:Malondialdehyde; D006861:Hydrogen Peroxide