title: Repulsive contribution of surface sialic acid residues to cell adhesion to substratum.
abstract: Roles of surface sialic acid residues in cell adhesion to substratum were investigated in model systems to clarify their contribution to intercellular interactions. Treatment of cells of a fibroblastic cell line, Swiss 3T3, an epithelial cell line, TES-1, and a T lymphoma cell line, BW5147, with a sialylated oligosaccharide binding lectin enhanced adhesion to plastic plates irrespective of the cell lineage. This enhancement was inhibited by the addition of sialyl lactose. Digestion of the cells with sialidase or endoglycoceramidase also augmented adhesion. On the other hand, adhesion was reduced by pre-coating plastic plates with gangliosides but not with desialylated gangliosides. These findings suggest that sialic acid residues exposed to cell surfaces negatively regulate cell adhesiveness.
authors.affiliation: Laboratory of Molecular Immunology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shimamura
authors.forename: N
authors.initials: N
authors.lastname: Shibuya
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Yamagata
mesh_terms: D016475:3T3 Cells / Q000166:cytology; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D004847:Epithelial Cells; D005732:Gangliosides; D037102:Lectins; D016399:Lymphoma, T-Cell; D051379:Mice; D008807:Mice, Inbred BALB C; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids; D013950:Thymus Gland / Q000166:cytology; D014407:Tumor Cells, Cultured
chemical_list: D005732:Gangliosides; D037102:Lectins; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid