title: Western blot analysis of thyrotropin receptor expression in human thyroid tumours and correlation with TSH-binding.
abstract: Expression of thyrotropin receptor (TSHr) was analyzed in normal human thyroids and differentiated thyroid tumours by Northern blotting of total RNA and by Western blotting of detergent-solubilized membrane proteins with an antibody specific for the extracellular domain of TSHr. Under nonreducing conditions TSHr stained as a 90-kDa protein in normal thyroid tissue. Expression varied in differentiated carcinomas from normal to not detectable in parallel to steady state TSHr mRNA levels and TSH binding in a TSH binding assay. The negative expression of TSHr in some patients with differentiated carcinomas may have consequences for the TSH suppressive treatments of these patients.
authors.affiliation: Department of Clinical Endocrinology, Medizinische Hochschule Hannover, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Pötter
authors.forename: R
authors.initials: R
authors.lastname: Horn
authors.forename: G F
authors.initials: GF
authors.lastname: Scheumann
authors.forename: H
authors.initials: H
authors.lastname: Dralle
authors.forename: S
authors.initials: S
authors.lastname: Costagliola
authors.forename: M
authors.initials: M
authors.lastname: Ludgate
authors.forename: G
authors.initials: G
authors.lastname: Vassart
authors.forename: J E
authors.initials: JE
authors.lastname: Dumont
authors.forename: G
authors.initials: G
authors.lastname: Brabant
mesh_terms: D001665:Binding Sites; D015153:Blotting, Western; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011989:Receptors, Thyrotropin / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000378:metabolism*; D013972:Thyrotropin / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011989:Receptors, Thyrotropin; D013972:Thyrotropin