Determination of aflatoxin Q1 in urine by automated immunoaffinity column clean-up and liquid chromatography.
A liquid chromatographic system with an automated clean-up procedure for aflatoxin Q1 in human urine is described. The samples were cleaned up by using immunoaffinity columns originally designed for aflatoxin M1. The chromatographic system was a C18 column with an acidic mobile phase of acetonitrile-water containing potassium bromide. Fluorescence detection (365/440 nm) of aflatoxin Q1 was enhanced by addition of bromine, using post-column derivatization, which was studied by factorial designs. Average recovery of aflatoxin Q1 in spiked 10-ml urine samples was 88% (R.S.D. = 6.4%) at a level of 50 pg/ml. The determination limit was 49.5 pg/ml urine.
Kussak
A
A
National Institute of Occupational Health, Analytical Chemistry Division, Umeå, Sweden.
Andersson
B
B
Andersson
K
K
D016607:Aflatoxin M1 / Q000276:immunology; D000348:Aflatoxins / Q000097:blood* / Q000652:urine*; D001322:Autoanalysis; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D006801:Humans; D007118:Immunoassay; D012015:Reference Standards; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins; C033759:aflatoxin Q1; D016607:Aflatoxin M1