title: Nucleosome-restricted antibodies are detected before anti-dsDNA and/or antihistone antibodies in serum of MRL-Mp lpr/lpr and +/+ mice, and are present in kidney eluates of lupus mice with proteinuria.
abstract: OBJECTIVE
  To compare the humoral response to nucleosomes with the response to their individual components (double-stranded DNA [dsDNA] and histones) and to assess the involvement of antinucleosome antibodies in immune deposits in the kidney of MRL mice.
  
  
  METHODS
  We used enzyme-linked immunosorbent assays of sera and kidney eluates for antibody activity against purified nucleosomes, dsDNA, and histones.
  
  
  RESULTS
  Antinucleosome antibodies emerged before anti-dsDNA and antihistone antibodies. A fraction of antinucleosome antibodies reacted exclusively with nucleosomes and not with their components, dsDNA and histones. These nucleosome-restricted antibodies were detected in the proteinuric MRL mouse kidney eluate.
  
  
  CONCLUSIONS
  Our findings support the notion that nucleosomes play a major role in the emergence of antinuclear autoantibodies and that antinucleosome antibodies might be involved in the nephritogenic process in murine lupus.
authors.affiliation: Unité INSERM 25, Hôpital Necker, Paris, France.
authors.forename: Z
authors.initials: Z
authors.lastname: Amoura
authors.forename: H
authors.initials: H
authors.lastname: Chabre
authors.forename: S
authors.initials: S
authors.lastname: Koutouzov
authors.forename: C
authors.initials: C
authors.lastname: Lotton
authors.forename: A
authors.initials: A
authors.lastname: Cabrespines
authors.forename: J F
authors.initials: JF
authors.lastname: Bach
authors.forename: L
authors.initials: L
authors.lastname: Jacob
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis* / Q000096:biosynthesis / Q000097:blood; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006657:Histones / Q000276:immunology*; D007668:Kidney / Q000276:immunology*; D008181:Lupus Nephritis / Q000150:complications / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D009707:Nucleosomes / Q000276:immunology*; D011507:Proteinuria / Q000209:etiology*; D013997:Time Factors
chemical_list: D000974:Antibodies, Antinuclear; D006657:Histones; D009707:Nucleosomes; D004247:DNA