title: Effect of Lewis blood group antigen expression on bacterial adherence to COS-1 cells.
abstract: Epithelial cells from secretor individuals demonstrate decreased bacterial adherence compared with cells from nonsecretors. Lewis blood group antigen expression is one component of the secretor/nonsecretor phenotype and several epidemiologic studies have suggested a link between Lewis blood group antigen phenotype and susceptibility to urinary tract infections. In this study, we examined the possibility that the expression of the difucosylated Lewis blood group determinants, Leb and Ley (associated with the secretor phenotype), made cells less susceptible to Escherichia coli adherence by masking receptors for pili. COS-1 cells, which do not produce Lewis (Lea, Leb, Le(x), and Ley) blood group antigens, were used as target cells for bacterial adherence. The surface blood group antigen expression pattern of the cells was then modified by cotransfection with plasmids containing DNA inserts encoding alpha (1,2)-fucosyltransferase and alpha (1,3)- and alpha (1,4)-fucosyltransferases, resulting in the expression of Leb and Ley. E. coli HB101 expressing various adhesins (type 1, PapJ96, PapIA2, PapAD110, Prs, and S) from recombinant plasmids bound equally well to untransfected cells and transfected cells expressing Lea and Le(x) (nonsecretor phenotype) and Leb and Ley (secretor phenotype) antigens. We conclude that the presence of Leb and Ley antigens on cells from secretors does not alone mask receptors for E. coli pili or hinder bacterial adherence.
authors.affiliation: Department of Urology, Northwestern University Medical School, Chicago, Illinois.
authors.forename: R A
authors.initials: RA
authors.lastname: Gaffney
authors.forename: A J
authors.initials: AJ
authors.lastname: Schaeffer
authors.forename: B E
authors.initials: BE
authors.lastname: Anderson
authors.forename: J L
authors.initials: JL
authors.lastname: Duncan
mesh_terms: D000818:Animals; D000941:Antigens / Q000096:biosynthesis*; D001422:Bacterial Adhesion / Q000502:physiology*; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D007983:Lewis Blood Group Antigens / Q000502:physiology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection
chemical_list: D000941:Antigens; D007983:Lewis Blood Group Antigens; D011994:Recombinant Proteins