title: Immunolocalization of microfibril and microfibril-associated proteins in the subendothelial matrix of the developing mouse aorta.
abstract: In the developing aorta, endothelial cell connecting filaments extend from the abluminal surface of the endothelial cell to the subjacent elastic lamina. The connecting filaments are in alignment with intracellular stress fibers and are oriented parallel to the direction of blood flow. In the present study, the composition of the endothelial cell connecting filaments was investigated by indirect immunogold labeling with antibodies to the microfibril proteins, MP340 (fibrillin) and MAGP, and to fibronectin and heparan sulfate proteoglycan (HSPG). In the subendothelial matrix of both 15-day gestational and 5-day post-natal mouse aortae, the connecting filaments showed moderate immunoreactivity with anti-MP340; however, no significant immunoreaction was seen with anti-MAGP. Anti-fibronectin strongly labeled the connecting filaments and a weak immunoreaction was seen with anti-HSPG. In contrast, the adjacent 'elastin-associated microfibrils' showed a very strong immunoreaction with anti-MP340 and a moderate reaction with anti-MAGP. Little or no reaction was seen with anti-fibronectin or anti-HSPG. The filaments that connect endothelial cells to the subjacent elastic lamina during aortic development are thus microfibrillar in nature and related to elastin-associated microfibrils as evidenced by their positive immunoreaction with anti-MP340. The absence of labeling with anti-MAGP, however, suggests that either these fibrillin-containing filaments do not contain MAGP or that the immunoreactive epitopes are blocked by the proteins that coat the connecting filaments such as fibronectin. These results suggest that microfibrils not in association with elastin may play a role in cell anchorage and, more specifically, in the aorta may be involved in maintaining the structural integrity of the endothelial cell layer during early development of the vessel wall. Furthermore, the absence of immunoreactivity with anti-MAGP on the fibrillin-containing endothelial cell connecting filaments raises the possibility that microfibrils may consist of a family of related filaments rather than a single structural entity.
authors.affiliation: Department of Anatomy, McGill University, Montreal, Quebec, Canada.
authors.forename: E C
authors.initials: EC
authors.lastname: Davis
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry*; D000818:Animals; D000831:Animals, Newborn; D001011:Aorta / Q000737:chemistry / Q000196:embryology / Q000254:growth & development; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development; D000071837:Fibrillins; D005353:Fibronectins / Q000032:analysis; D005865:Gestational Age; D006497:Heparan Sulfate / Q000032:analysis; D007150:Immunohistochemistry; D051379:Mice; D008840:Microfilament Proteins / Q000032:analysis*
chemical_list: D000071837:Fibrillins; D005353:Fibronectins; D006497:Heparan Sulfate; D008840:Microfilament Proteins