Ki-ras and c-myc oncogene expression measured by coamplification polymerase chain reaction.
Ki-ras and c-myc oncogene mRNA in carcinoma tissue was quantitatively detected by the coamplification polymerase chain reaction. After the reverse transcription of the mRNA mixture with random hexanucleotide primer the coamplification polymerase chain reaction of the oncogene and the internal standard beta-actin cDNA was done. The amount of oncogene mRNA was calculated from the coamplified product ratio. Ki-ras expression in a human gastric cancer strain H-111 was estimated to 5 percent of beta-actin. The expression of c-myc mRNA in colorectal carcinoma tissue was observed to be ten to one hundred times higher than normal mucosa. Gene expression was compared numerically in mole/liter without using hybridization and radioactive probes.
Sugimoto
T
T
Department of Oncologic Surgery, School of Medicine, Osaka University, Japan.
Tsukamato
F
F
Fujita
M
M
Takai
S
S
D000199:Actins / Q000096:biosynthesis; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D005753:Gastric Mucosa / Q000378:metabolism; D015870:Gene Expression; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis; D013274:Stomach Neoplasms; D013698:Templates, Genetic; D014407:Tumor Cells, Cultured
D000199:Actins; D017931:DNA Primers; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger