title: Crystallization and preliminary X-ray diffraction studies of mandelonitrile lyase from almonds.
abstract: Single crystals of three different isoenzymes of (R)-(+) mandelonitrile lyase (hydroxynitrile lyase) from almonds (Prunus amygdalus) have been obtained by hanging drop vapor diffusion using polyethylene glycol 4000 and isopropanol as co-precipitants. The crystals belong to the monoclinic space group P2(1) with unit cell parameters a = 69.9, b = 95.1, c = 95.6 A, and beta = 118.5 degrees. A complete set of diffraction data has been collected to 2.6 A resolution on native crystals of isoenzyme III.
authors.affiliation: Institut für Organische Chemie und Isotopenforschung, Universität Stuttgart, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Lauble
authors.forename: K
authors.initials: K
authors.lastname: Müller
authors.forename: H
authors.initials: H
authors.lastname: Schindelin
authors.forename: S
authors.initials: S
authors.lastname: Förster
authors.forename: F
authors.initials: F
authors.lastname: Effenberger
mesh_terms: D000446:Aldehyde-Lyases / Q000737:chemistry* / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification; D009754:Nuts / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D000446:Aldehyde-Lyases; C021541:mandelonitrile lyase