Molecular forms of circulating atrial natriuretic peptides in human plasma and their metabolites.
High performance gel permeation chromatography (HP-GPC) followed by four radioimmunoassays (RIAs) devised to amino acids (a.a.) 1-30, 31-67, 79-98, and 99-126 of the 126 a.a. atrial natriuretic factor (ANF) prohormone revealed that the proANF(1-30) assay immunoreactivity in plasma is 50% proANF(1-30) and 50% proANF(1-98). The HP-GPC evaluation of plasma followed by proANF(31-67) and ANF [i.e., proANF(99-126)] assays revealed that proANF(31-67) and ANF circulate as distinct peptides. the HP-GPC plasma examination followed by proANF(79-98) assay immunologically recognized three peaks in plasma consistent with proANF(1-98), -(68-98), and -(79-98). Similar HP-GPC evaluation of urine followed by these RIAs indicated that the proANF(1-30), -(79-98), and ANF assays only recognize 500 mol.wt. or less peptides, and the proANF(31-67) RIA recognizes a nearly intact proANF(31-67) with only two to three amino acids removed during processing of this peptide.
Gower
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WR
Department of Biochemistry, University of South Florida Health Sciences Center, and James A. Haley Veterans Hospital, Tampa 33612.
Chiou
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Skolnick
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Vesely
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DL
D000328:Adult; D009320:Atrial Natriuretic Factor / Q000737:chemistry / Q000378:metabolism*; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D011863:Radioimmunoassay; D012016:Reference Values
D010446:Peptide Fragments; D009320:Atrial Natriuretic Factor