Mapping of two phenol sulphotransferase genes, STP and STM, to 16p: candidate genes for Batten disease.
The cytosolic phenol sulphotransferase gene (STP) was mapped to a region of chromosome 16, within the interval defined by human-rodent somatic cell hybrid breakpoints CY160(D) and CY12, which contains FRA16E. YAC and cosmid clones from this 16p interval were screened for the presence of STP. Two non-overlapping cosmid contigs were identified which contain STP-like sequences. Sequencing of these STP-like sequences confirmed that STP is contained within contig 343.1 and maps proximal to FRA16E, and that a related sulphotransferase STM, encoding the catecholamine-sulphating enzyme, is contained within contig 55.4 and maps to the adjacent hybrid interval CY12-CY180A. Thus two phenol sulphotransferase genes (STP and STM) have been finely localised to chromosome 16p12.1-p11.2, to the same region as CLN3, the gene for Batten disease. Both genes are therefore candidate genes for Batten disease.
Dooley
T P
TP
Department of Genetics, Sowthwest Foundation for Biomedical Research, San Antonio, Texas.
Mitchison
H M
HM
Munroe
P B
PB
Probst
P
P
Neal
M
M
Siciliano
M J
MJ
Deng
Z
Z
Doggett
N A
NA
Callen
D F
DF
Gardiner
R M
RM
D000818:Animals; D015239:Arylsulfotransferase / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D003360:Cosmids; D017931:DNA Primers; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009472:Neuronal Ceroid-Lipofuscinoses / Q000235:genetics*; D012377:Rodentia
D017931:DNA Primers; D015239:Arylsulfotransferase