[The use of the polymerase chain reaction method for the analysis of polymorphic sections of the human genome in performing forensic medical expertise on material evidence].
The method of expert evaluation is described which was carried out to define the appurtenance of seminal spots to one of two suspected subjects. Biological sample appurtenance was identified using genetic markers by analysis of allele status of loci containing variable numbers of tandem repeats. Two loci were analyzed, ApoB and IL-6. To determine the studied loci alleles polymerase chain reaction with the DNA isolated from material evidences used as the matrix was used as well as agarose gel electrophoresis. Analysis helped determine appurtenance of seminal spots to suspected subject V.
Kotliarova
S E
SE
Kovalenko
S P
SP
Sharonova
D A
DA
Novoselov
V P
VP
D000017:ABO Blood-Group System; D004247:DNA / Q000302:isolation & purification; D005104:Expert Testimony / Q000379:methods*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011902:Rape; D013094:Spermatozoa
D000017:ABO Blood-Group System; D004247:DNA
Primenenie metoda polimeraznoĭ tsepnoĭ reaktsii dlia analiza polimorfnykh uchastkov genoma cheloveka pri provedenii sudebno-meditsinskoĭ ékspertizy veshchestvennykh dokazatel'stv.