[The protective activity of Yersinia pseudotuberculosis antigens].
Samples of Y.pseudotuberculosis (serovar I) antigens, represent a high-molecular lipopolysaccharide (LPS) fraction with a mol. wt. of 22.5 kD and fractions of outer membrane proteins isolated by the method of M. Osborn and R. Munson (1974), were tested in comparison with the activity with live cells of Y. pseudotuberculosis I attenuated mutant KV 9/2, having lost its Cad plasmid of virulence with a mol. wt. of 47 MD and carrying 2 attenuating markers: resistance to crystal violet and nalidixic acid. In experiments on guinea pigs pathomorphological studies demonstrated high protective activity of Y.pseudotuberculosis I attenuated mutant KV 9/2 and a pronounced protective effect achieved after the immunization of the animals with complex biopolymers, including a high-molecular LPS fraction and outer membrane proteins.
Tseneva
G Ia
GIa
Bondarenko
V M
VM
Voskresenskaia
E A
EA
Kuliashova
L B
LB
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000276:immunology / Q000302:isolation & purification; D004353:Drug Evaluation, Preclinical; D006168:Guinea Pigs; D007114:Immunization; D008070:Lipopolysaccharides / Q000276:immunology / Q000302:isolation & purification; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000276:immunology / Q000302:isolation & purification; D015011:Yersinia pseudotuberculosis / Q000276:immunology* / Q000302:isolation & purification; D015012:Yersinia pseudotuberculosis Infections / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D014613:Vaccines, Attenuated
Protektivnaia aktivnost' antigenov Yersinia pseudotuberculosis.