Crystallization and preliminary crystallographic data for Rab guanine nucleotide dissociation inhibitor (RabGDI) from bovine brain.
X-ray quality crystals of Rab guanine nucleotide dissociation inhibitor (RabGDI) from bovine brain expressed in Escherichia coli have been obtained from 1.73 M ammonium sulfate. The crystals are prismatic long rods and belong to the monoclinic space group P21 with approximate cell dimensions a = 91.9 A, b = 43.5 A, c = 63.2 A, beta = 104.5 degrees and one molecule per asymmetric unit. The crystals are stable in the X-ray beam and diffract to at least 2.3 A. Reverse screening, streak seeding and macroseeding methods were used to obtain and improve the crystals.
Schalk
I J
IJ
Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037.
Stura
E A
EA
Matteson
J
J
Wilson
I A
IA
Balch
W E
WE
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D018360:Crystallography, X-Ray; D019204:GTP-Binding Proteins / Q000737:chemistry*; D020730:Guanine Nucleotide Dissociation Inhibitors; D020692:rab3 GTP-Binding Proteins
C086796:GDP dissociation inhibitor 1; D020730:Guanine Nucleotide Dissociation Inhibitors; D019204:GTP-Binding Proteins; D020692:rab3 GTP-Binding Proteins