title: Light chains of immunoglobulins in human secretions.
abstract: Immunoglobulin kappa light chains are predominant in normal human serum and a kappa/lambda ratio of 1.7 to 2 has been reported. However, little is known of the partition of light chains in secretions. The levels of IgA, kappa and lambda were assayed by nephelometry in a series of secretions and in normal human serum. Although kappa chains remained predominant, the different secretions studied could be classified according to their kappa/lambda ratio. In saliva (P < 0.001), nasal fluid (P = 0.01) and tears (P = 0.04) the kappa/lambda ratio was significantly lower than in serum. Conversely, higher kappa/lambda ratios were obtained in gastric juice (P = 0.005) and hepatic bile (P = 0.004) and no significant difference was noted between serum and gall bladder bile (P = 0.62). The lower ratios were all observed in fluids produced by glands surrounded by lymphoid tissue included in the mucosae-associated lymphoid tissue. These data strengthen previous observations that suggest a preferential production of lambda chains in human mucosae.
authors.affiliation: Laboratoire d'Immunologie, GRIP, Faculté de Médecine, Vandoeuvre, France.
authors.forename: C M
authors.initials: CM
authors.lastname: Molé
authors.forename: M C
authors.initials: MC
authors.lastname: Béne
authors.forename: P M
authors.initials: PM
authors.lastname: Montagne
authors.forename: E
authors.initials: E
authors.lastname: Seilles
authors.forename: G C
authors.initials: GC
authors.lastname: Faure
mesh_terms: D000328:Adult; D001826:Body Fluids / Q000737:chemistry*; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D007146:Immunoglobulin lambda-Chains / Q000032:analysis; D009391:Nephelometry and Turbidimetry
chemical_list: D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains