Preliminary characterization of the structural proteins of the coronaviruses, sialodacryoadenitis virus and Parker's rat coronavirus.
A procedure was developed for the partial purification of the rat coronaviruses, sialodacryoadenitis virus (SDAV) and Parker's rat coronavirus (PRC). The SDAV and PRC were replicated in L-2 cell monolayer cultures, precipitated with ammonium sulphate, and further concentrated using sucrose density gradient centrifugation. The major SDAV and PRC proteins were identified by immunoblotting and compared with those of the JHM strain of mouse hepatitis virus (MHV-JHM). Monoclonal antibodies (MAb) against the M protein of JHM recognized proteins interpreted to be slightly smaller in immunoblots of SDAV and PRC (22.8 vs 23K for JHM). Similarly, a monoclonal antibody against the JHM N protein reacted with proteins of 53K in SDAV and PRC (vs 56 K for JHM). Polyclonal antisera to all three viruses also cross-reacted with the M and N proteins. Some cross-reactivity amongst the S proteins was observed. Based on these data, the structural proteins of the rat coronaviruses, SDAV and PRC are closely related to those of MHV-JHM.
Barker
M G
MG
Department of Pathology, University of Guelph, Ontario.
Percy
D H
DH
Hovland
D J
DJ
MacInnes
J I
JI
D000645:Ammonium Sulfate; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D017941:Coronavirus, Rat / Q000737:chemistry*; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D008970:Molecular Weight; D051381:Rats; D015678:Viral Structural Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D015678:Viral Structural Proteins; D000645:Ammonium Sulfate