title: Structural investigation of the capsular polysaccharide of Escherichia coli O101 : K103 : H- using bacteriophage degradation and NMR spectroscopy.
abstract: NMR spectroscopy was performed on the depyruvated capsular antigen of E. coli K103 and on the oligosaccharide obtained by depolymerisation of the native polysaccharide with a viral-borne endoglycanase. This capsular polysaccharide is the only one to be co-expressed with O group 101 and joins a small group of unusual capsular polysaccharides which possess pyruvic acid as the only acidic function. The primary structure was shown to be composed of the repeating unit: [formula: see text]
authors.affiliation: School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
authors.forename: M R
authors.initials: MR
authors.lastname: Grue
authors.forename: H
authors.initials: H
authors.lastname: Parolis
authors.forename: L A
authors.initials: LA
authors.lastname: Parolis
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry* / Q000378:metabolism; D001435:Bacteriophages / Q000378:metabolism*; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure