title: A simple method for obtaining highly viable virus from culture supernatant.
abstract: Traditionally density-gradient methods are used to purify viruses. However, these procedures are not only time consuming and cumbersome, recovery of viable viruses are often quite low. In this report, a single-step concentration technique was used to concentrate a mutant of Moloney murine leukemia virus (ts1) virus from culture supernatants by ultrafiltration. A special ultrafiltration unit with a 100,000 mol wt cut-off was able to concentrate viruses about 30-fold without losing any infectivity. In comparison, traditional sucrose density gradient purified viruses lost a significant portion of their infectivity. This technique could be used for concentrating other viruses for many useful purposes where more viable viruses are needed, e.g., study of virus-cell binding.
authors.affiliation: University of Texas, M.D. Anderson Cancer Center, Science Park-Research Division, Smithville 78957.
authors.forename: K
authors.initials: K
authors.lastname: Saha
authors.forename: Y C
authors.initials: YC
authors.lastname: Lin
authors.forename: P K
authors.initials: PK
authors.lastname: Wong
mesh_terms: D002460:Cell Line; D008979:Moloney murine leukemia virus / Q000254:growth & development / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000379:methods*