Air samples from a building with asbestos-containing material: asbestos content and in vitro toxicity on rat pleural mesothelial cells.
Buildings equipped with asbestos-containing material may release asbestos fibers, the toxicity of which remains uncertain because of the generally low dose level. In 22 air samples collected in a building the asbestos level ranged between 0 and 0.027 f/ml. Both chrysotile and amphiboles were found. There was no association between in vitro cytotoxicity on rat pleural mesothelial cells and asbestos content, but there was a significant correlation with the total amount of particulate material. Four samples exhibited an enhancement of DNA synthesis in cells arrested in G1 with 5 mM hydroxyurea. This is more likely related to the particulate matter associated with asbestos fibers.
Dong
H
H
Laboratory of Environmental Cellular and Molecular Pathology, Institut National de la Santé et de la Recherche Médicale (INSERM) U. 139, CHU Henri Mondor, Créteil, France.
Saint-Etienne
L
L
Renier
A
A
Billon Galland
M A
MA
Brochard
P
P
Jaurand
M C
MC
D016902:Air Pollution, Indoor / Q000032:analysis*; D000818:Animals; D001194:Asbestos / Q000032:analysis* / Q000633:toxicity*; D017638:Asbestos, Crocidolite / Q000032:analysis / Q000633:toxicity; D017632:Asbestos, Serpentine / Q000032:analysis / Q000633:toxicity; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D009153:Mutagens / Q000633:toxicity; D010994:Pleura / Q000187:drug effects*; D051381:Rats; D013778:Tetrazolium Salts; D013844:Thiazoles
D017632:Asbestos, Serpentine; D009153:Mutagens; D013778:Tetrazolium Salts; D013844:Thiazoles; D017638:Asbestos, Crocidolite; D001194:Asbestos; D004247:DNA; C022616:thiazolyl blue