Lipophorin as a lipid carrier in the hemolymph of the horseshoe crab, Limulus polyphemus.
A high-density lipoprotein (HDL) was obtained from the hemolymph of Limulus polyphemus in yields generally less than 30 micrograms/ml by ultracentrifugal flotation. SDS-PAGE revealed two apolipoproteins with masses similar to those of apolipophorins (apoLp-I, 265 +/- 14 kDa; apoLp-II, 89 +/- 6 kDa). Lipid composition was different from both insect lipophorin and crustacean HDL, and showed less diacylglycerols than triacylglycerols (3.8% and 36.2% of total lipids, respectively). Since Limulus polyphemus is closely related to precambrian chelicerates, our results confirm that lipophorin was present early in the evolution of arthropods.
Rehn
K G
KG
Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brasil.
Lee
R F
RF
D000818:Animals; D001053:Apolipoproteins / Q000097:blood / Q000302:isolation & purification; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D006458:Hemolymph / Q000737:chemistry*; D006737:Horseshoe Crabs; D008074:Lipoproteins; D008075:Lipoproteins, HDL / Q000097:blood / Q000302:isolation & purification; D014461:Ultracentrifugation / Q000379:methods
D001053:Apolipoproteins; D002352:Carrier Proteins; D008074:Lipoproteins; D008075:Lipoproteins, HDL; C032830:lipophorin