Signalling via MHC class II molecules selectively induces IL-1 beta over IL-1 receptor antagonist gene expression.
Activation of human monocytes or human monocytic cell lines by several types of stimuli coordinately induces IL-1 beta and its antagonist (IL-1Ra) gene expression; alterations in their balance seem to mediate the inflammatory response. Using the human monocytic cell line THP-1, we report that superantigens, such as staphylococcal enterotoxin A (SEA) and Mycoplasma arthritidis -derived superantigen (MAM) induce an increase in the level of IL-1 beta mRNA without any detectable effect on IL-Ra mRNA. Unlike MAM-induced IL-1 beta mRNA, SEA-induced IL-1 beta mRNA was adequately translated into protein. Superantigen-induced gene expression is mediated by signalling, via their receptors, the MHC class II molecules. Thus, it appears that this mode of signalling selectively induces the proinflammatory cytokine IL-1 beta gene expression which, by itself, can have major importance in disease pathology especially in autoimmune diseases.
al-Daccak
R
R
Department of Medicine, Université Laval, St-Foy, Québec, Canada.
Mehindate
K
K
Poubelle
P E
PE
Mourad
W
W
D000818:Animals; D000941:Antigens; D000942:Antigens, Bacterial; D015152:Blotting, Northern; D002460:Cell Line; D004768:Enterotoxins / Q000494:pharmacology; D015870:Gene Expression; D006681:HLA-D Antigens / Q000502:physiology*; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000096:biosynthesis*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology; D009000:Monocytes; D011506:Proteins; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins; D012795:Sialoglycoproteins / Q000096:biosynthesis*; D015398:Signal Transduction; D013211:Staphylococcus aureus; D018089:Superantigens / Q000494:pharmacology*
D000941:Antigens; D000942:Antigens, Bacterial; D004768:Enterotoxins; D006681:HLA-D Antigens; C507763:IL1RN protein, human; C507764:Il1rn protein, mouse; D007369:Interferon Inducers; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008934:Mitogens; C052996:Mycoplasma arthritidis mitogen; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D018089:Superantigens; C026066:enterotoxin A, Staphylococcal; D007371:Interferon-gamma