title: Effects of herpes simplex virus type 1 infection on the plasma membrane and related functions of HeLa S3 cells.
abstract: In this study we evaluated modifications of various structural and functional properties of the plasma membrane of HeLa S3 cells following infection by the lytic virus herpes simplex virus type 1 (HSV-1). Na+/K(+)-ATPase activity considerably decreased during the first few hours post-infection (p.i.), whereas Na+ and K+ concentrations were not significantly affected until a much later period. By 8 h p.i., a partial membrane depolarization in infected cells had occurred, as indicated by a small change in the transmembrane potential. HSV infection induced a time-dependent lipid peroxidation of HeLa cell plasma membranes temporally correlated with the progressive reduction in Na+/K(+)-ATPase activity. Moreover, a significant decrease of membrane fluidity appeared at a late phase of the viral replicative cycle probably representing cumulative membrane damage. These results demonstrate that HSV-1 infection induced the production of free radicals in non-phagocytic cells. Since lipid peroxidation begins at an early stage of the virus replicative cycle, it may be directly related to viral cytopathicity.
authors.affiliation: Institute of Microbiology, University of Padova Medical School, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Palù
authors.forename: M A
authors.initials: MA
authors.lastname: Biasolo
authors.forename: G
authors.initials: G
authors.lastname: Sartor
authors.forename: L
authors.initials: L
authors.lastname: Masotti
authors.forename: E
authors.initials: E
authors.lastname: Papini
authors.forename: M
authors.initials: M
authors.lastname: Floreani
authors.forename: P
authors.initials: P
authors.lastname: Palatini
mesh_terms: D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology* / Q000821:virology; D002463:Cell Membrane Permeability; D003588:Cytopathogenic Effect, Viral; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D005456:Fluorescent Dyes; D005609:Free Radicals; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D008560:Membrane Fluidity; D008564:Membrane Potentials; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014779:Virus Replication
chemical_list: D005456:Fluorescent Dyes; D005609:Free Radicals; D004161:Diphenylhexatriene; D008315:Malondialdehyde; C037385:1-(4'-carboxyethyl)-6-diphenyl-1,3,5-hexatriene; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium