[Inhibitory effect of interferon on expression of oncogene in over-proliferative smooth muscle cell].
The model of balloon angioplasty (BA) was founded in rabbit abdominal aorta. In situ hybridization was used with the probes of oncogenes c-fos, c-myc and N-ras labeled by digoxin in vessel segments after BA and with the probes of oncogenes c-fos, c-myc, and N-ras labeled by biotin in smooth muscle cell (SMC) irritated by cultured medium of vessel segments after BA. Overexpression of c-fos, c-myc and N-ras oncogenes occurred in vessel segments after BA mainly in atherosclerotic plaques. There was expression of oncogene in normal vessel segments. Overexpression appeared in the over-proliferative SMC irritated by cultured medium of vessel segments after BA. A expression of N-ras occurred on the membrane of cellular nucleus. There was no expression of oncogenes in the normal SMC of control group. Overexpression could be partly inhibited by interferon-gamma.
Dong
R
R
Division of Cardiology, First Hospital, Beijing Medical University.
Wang
L I
LI
D000800:Angioplasty, Balloon / Q000009:adverse effects*; D000818:Animals; D001161:Arteriosclerosis / Q000235:genetics*; D002478:Cells, Cultured; D015870:Gene Expression; D016762:Genes, fos; D016259:Genes, myc; D011905:Genes, ras; D006965:Hyperplasia; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D011519:Proto-Oncogenes; D011817:Rabbits
D007371:Interferon-gamma