title: Induction of ovulation in rabbits by pure urinary luteinizing hormone.
abstract: In 18-week-old nulliparous rabbit does, ovulation was induced with 50 IU of pure urinary luteinizing hormone (LH; LH group), or 50 IU of human chorionic gonadotrophin (HCG; HCG group), in order to determine the effect of these treatments on 17 beta-oestradiol and progesterone concentrations, and on oocyte and embryo quality. Luteinizing follicles, recovered oocytes, progesterone concentration and grade 5 embryos were significantly reduced when pure urinary LH was used. Statistically significant correlations were found: (i) between oestradiol concentration and number of degenerated oocytes in both groups (positive); (ii) between oestradiol concentration and grade 1 and 2 embryos (negative), and grade 5 embryos (positive) in the HCG group; (iii) between progesterone concentration and metaphase II oocytes (negative), and between progesterone and grade 5 embryos (positive), in the HCG group; and (iv) between progesterone and oestradiol concentrations (negative) in the LH group. It seems that the oestradiol to progesterone ratio improves during the early luteal phase when ovulation is induced with LH, and that oestradiol and progesterone concentrations could play a role in determining oocyte and embryo quality.
authors.affiliation: Unidad de Reproducción Humana, Hospital Universitario La Fe, Valencia, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Molina
authors.forename: A G
authors.initials: AG
authors.lastname: Santana
authors.forename: M
authors.initials: M
authors.lastname: Plá
authors.forename: A
authors.initials: A
authors.lastname: Romeu
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000097:blood; D005260:Female; D007986:Luteinizing Hormone / Q000494:pharmacology* / Q000652:urine; D010053:Ovary / Q000033:anatomy & histology; D010062:Ovulation Induction / Q000379:methods*; D011374:Progesterone / Q000097:blood; D011817:Rabbits; D014599:Uterus / Q000033:anatomy & histology
chemical_list: D006063:Chorionic Gonadotropin; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone