title: Histobacteriological analysis of acid red dye-stainable dentin found beneath intact amalgam restorations.
abstract: This study determined histologically the incidence of acid red dye-stainable dentin and its correlation to bacterial presence beneath intact clinically sound amalgam restorations. Fourteen of 16 clinically sound amalgam restorations (88%), serving an average of 11 years, had microscopically evident microorganisms in the subjacent dentin. Eleven of the 14 specimens containing bacteria (79%) exhibited acid red dye-stainable dentin. There was no obvious correlation between dye-stainable dentin and the presence of bacteria on the walls of the cavity preparations studied. The question of how much dye-stainable dentin can be present beneath an amalgam restoration before the restoration fails is still unanswered.
authors.affiliation: Temple University School of Dentistry, Department of Operative Dentistry, Philadelphia, PA 19140.
authors.forename: D W
authors.initials: DW
authors.lastname: Boston
authors.forename: H T
authors.initials: HT
authors.lastname: Graver
mesh_terms: D000328:Adult; D000368:Aged; D003723:Dental Amalgam; D003731:Dental Caries / Q000175:diagnosis*; D003732:Dental Caries Activity Tests; D003793:Dental Restoration, Permanent; D003804:Dentin / Q000382:microbiology*; D006801:Humans; D008875:Middle Aged; D012235:Rhodamines
chemical_list: D012235:Rhodamines; C022027:lissamine rhodamine B; D003723:Dental Amalgam