Development of a candidate vaccine against Ross River virus infection.
Ross River virus is a mosquito-borne alphavirus which causes several thousand cases of arthritis (epidemic polyarthritis) each year. In this study, binary ethylenimine (BEI) was used to destroy the infectivity of this virus without abolishing the antigenicity or immunogenicity of the virion. Mice immunized intramuscularly with BEI-inactivated virus, with or without Alhydrogel adjuvant, produced antibody which neutralized Ross River virus in vitro, and the mice also failed to develop viraemia when challenged intravenously with live virus. Serum neutralization and in vivo protection were greatest when BEI-inactivated virus was administered without adjuvant.
Yu
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World Health Organization Collaborative Centre for Arbovirus Reference and Research, School of Life Science, Queensland University of Technology, Brisbane, Australia.
Aaskov
J G
JG
D000276:Adjuvants, Immunologic; D018354:Alphavirus Infections / Q000517:prevention & control / Q000821:virology*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001170:Arthritis, Infectious / Q000517:prevention & control / Q000821:virology*; D001388:Aziridines / Q000276:immunology / Q000494:pharmacology; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007117:Immunization, Secondary; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000473:pathology; D009500:Neutralization Tests; D012398:Ross River virus / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014709:Vero Cells; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D001388:Aziridines; D007106:Immune Sera; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; C033132:aziridine