[Species-specific monoclonal antibodies against Chlamydia pneumoniae].
The purified elementary bodies (EBs) of C. pneumoniae ATCC VR1310 were used for primary immunization of male BALB/c mice (8 weeks of age), with the boost following on day 14. Spleen cells were fused with murine myeloma NS-1 cells on day 24 and hybrid cells were cloned by limiting dilution. Two clones, P17C2 & P33C, secreting species-specific monoclonal antibodies (MAbs) and immunoglobulin IgG2a class were obtained after elimination of those clones that produced antibodies against C. trachomatis L1, L2, A, B, C, E serovars, C. psittaci EAE strain and uninfected BGMK cell antigens. It was showed that the two clones of MAbs reacted with the MOMP 39.5 x 10(3) Da major outer membrane protein of all chlamydia species in Western blot and their ascites titers were more than 1:25 600 in Micro-IF test.
Ni
A P
AP
Department of Clinical Laboratories, Peking Union Medical College Hospital, Beijing.
Everson
S
S
Li
Y Z
YZ
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D006825:Hybridomas / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal