title: Concentration of autoantibodies to native 60-kd Ro/SS-A and denatured 52-kd Ro/SS-A in eluates from the heart of a child who died with congenital complete heart block.
abstract: OBJECTIVE
  To determine the serologic specificity of acid eluates from tissues of a child who died with congenital complete heart block (CCHB).
  
  
  METHODS
  Tissues were extracted, acid eluted, and the IgG and antibody titers determined on the eluates by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Antibodies to native 60-kd and denatured 52-kd Ro/SS-A were found to be enriched only in the heart eluate, and not in the eluates from brain, kidney, and skin.
  
  
  CONCLUSIONS
  These findings indicate a major role for anti-native 60-kd Ro/SS-A in the immunopathogenesis of CCHB.
authors.affiliation: Oklahoma Medical Research Foundation, Oklahoma City 73104.
authors.forename: M
authors.initials: M
authors.lastname: Reichlin
authors.forename: A
authors.initials: A
authors.lastname: Brucato
authors.forename: M B
authors.initials: MB
authors.lastname: Frank
authors.forename: P J
authors.initials: PJ
authors.lastname: Maddison
authors.forename: V R
authors.initials: VR
authors.lastname: McCubbin
authors.forename: M
authors.initials: M
authors.lastname: Wolfson-Reichlin
authors.forename: L A
authors.initials: LA
authors.lastname: Lee
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology* / Q000378:metabolism; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005313:Fetal Death / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006327:Heart Block / Q000151:congenital* / Q000276:immunology*; D006801:Humans; D008970:Molecular Weight; D009206:Myocardium / Q000276:immunology*; D011247:Pregnancy; D011489:Protein Denaturation; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000276:immunology* / Q000378:metabolism; D000099207:SS-A Antigen
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human