DNA sequencing with a hexamer string primer and dye-labeled terminators.
A method for fluorescence-based DNA sequencing using a hexamer string primer and dye-labeled terminators is described. Sequencing protocols have been determined that provide an average read length of 393 bases with 99.6% base-calling accuracy for single-stranded M13mp18 DNA, and an average read length of 367 bases with 99.4% base-calling accuracy for double-stranded M13mp19 DNA. The signal intensity is stronger than that obtained with a standard 18-mer primer, while the resolution and length of readable sequence are comparable.
Hou
W
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Department of Chemistry, University of Wisconsin-Madison 53706.
Smith
L M
LM
D017931:DNA Primers / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D005453:Fluorescence; D017422:Sequence Analysis, DNA / Q000379:methods*
D017931:DNA Primers; D004277:DNA, Single-Stranded