[Inhibitory analysis of DNA polymerase of herpes simplex type 1].
The inhibitory potency of new analogs of nucleoside 5'-triphosphates modified at the sugar residue and or alpha-phosphate against herpes simplex virus type 1 DNA polymerase has been evaluated in a cell-free system containing M13mp10 phage DNA and a synthetic primer. Triphosphates of new acyclic nucleosides [1-(5-hydroxy-2-cis-pentenyl)nucleosides] were the most effective inhibitors among 15 types of nucleoside 5'-triphosphates under investigation, being threefold less active than acyclovirtriphosphate. 5'-Phosphonylmethyl-2'-deoxythymidine beta, gamma-diphosphate proved to be a poor substrate for DNA polymerase. Compounds with other modifications at alpha-phosphate were inactive. Constants of hydrolysis rate of acyclonucleosides incorporated into the 3' end of primer were determined.
Kukhanova
M K
MK
Kuznetsova
E V
EV
Ias'ko
M V
MV
O'Hara
B
B
Bekker
J
J
Morin
J
J
Gluzman
Y a
Ya
D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002474:Cell-Free System; D002478:Cells, Cultured; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D008969:Molecular Sequence Data; D019384:Nucleic Acid Synthesis Inhibitors; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics; D018411:Spodoptera
D004279:DNA, Viral; D019384:Nucleic Acid Synthesis Inhibitors
Ingibitornyĭ analiz DNK-polimerazy virusa gerpesa prostogo tipa 1.