Structure of low-molecular-weight chondroitinsulfate.
The structure of low-molecular-weight chondroitinsulfate (LMW-ChS, principal component of Matrix) was elucidated by a combination of physico-chemical and enzymatic methods. High-field 1H- and 13C-NMR spectra provide a characteristic fingerprint of this glycosaminoglycan. From the relative intensity of typical signals, the distribution of the sulfate group of LMW-ChS was shown to be 75.5% on O-6, and 27.5% on O-4 of the galactosamine unit. Together with the HPLC profiles, the 13C-NMR spectra also allowed, from the intensity of typical signals of end-residues, the determination of the average molecular weight and the molecular-weight distribution of LMW-ChS. This structural analysis is consistent with results obtained by enzymatic degradation with chondroitinases and analysis (by chromatography and NMR) of fragments.
Naggi
A
A
G. Ronzoni Institute of Chemistry and Biochemistry, Milan, Italy.
D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000648:ultrastructure; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D012016:Reference Values
D010446:Peptide Fragments; D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases