Hormonal control of exocrine pancreatic secretion in the isolated intact rat pancreas.
This study employs the isolated perfused rat pancreas to investigate the actions of the two gut hormones, cholecystokinin-octapeptide (CCK-8) and secretin on pancreatic juice flow and total protein output. Perfusion of the pancreas with either CCK-8 (100 pM) or secretin (100 pM) resulted in marked both pancreatic juice flow and total protein output. The effect of CCK-8 was three-fold larger compared to the responses obtained with secretin alone. Simultaneous application of CCK-8 and secretin (all 100 pM) failed to elicit either a potentiation or an additive effect in both pancreatic juice flow and total protein output. The net increases in both secretory parameters were reduced during combined application of CCK-8 and secretin compared to the responses obtained with CCK-8 alone. The results indicate that optimal concentrations of either CCK-8 or secretin can display marked secretagogue effects on the exocrine pancreas but when administered simultaneously they failed to elicit either an additive response or a potentiation in pancreatic juice secretion.
Camello
P J
PJ
Departamento de Fisiología Facultad de Veterinaria Universidad de Extremadura Cáceres, Spain.
Wisdom
D M
DM
Singh
J
J
Salido
G M
GM
D000818:Animals; D004347:Drug Interactions; D010179:Pancreas / Q000378:metabolism*; D010189:Pancreatic Juice / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012633:Secretin / Q000494:pharmacology*; D012636:Secretory Rate / Q000187:drug effects; D012844:Sincalide / Q000494:pharmacology*
D011506:Proteins; D012633:Secretin; D012844:Sincalide