Characterization of the gene for human neutrophil-activating peptide 78 (ENA-78).
The genomic DNA for ENA-78 has been obtained from a human chromosome 4 flow-sorted cosmid library. Three out of 25,000 screened single colonies yielded the same 2.2-kB EcoRI ENA-78 gene fragment. A similar size fragment was observed on genomic southern blots, suggesting the presence of a single ENA-78 gene. The transcriptional start site was localized using a 5' RACE protocol on first strand cDNA prepared from stimulated alveolar type-II epithelial cell (A549) poly(A) mRNA. The ENA-78 gene contains four exons and three introns and the open reading frame of 342 nucleotides encodes for a protein of total 114 amino acids. The 5' flanking region contains potential binding sites for several nuclear factors such as AP-2, NF-kappa B, and interferon regulatory factor-1.
Corbett
M S
MS
Theodor Kocher Institute, University of Bern, Switzerland.
Schmitt
I
I
Riess
O
O
Walz
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D016209:Interleukin-8 / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism
C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D018076:DNA, Complementary; D016209:Interleukin-8; D014157:Transcription Factors