Immunochemical analysis of some proteins in cerebrospinal fluid and serum of patients with ischemic strokes.
Immunochemical studies of gamma gamma-neuron specific enolase (NSE), parvalbumin (PV), S-100 protein (S-100) and acidic fibrillary glial protein (GFAP) were studied in the cerebrospinal fluid and blood serum in 7 patients with ischemic cerebral stroke, aged 57 to 81 years. Cerebrospinal fluid and the first blood sample were taken on the first or second day of the disease. Further blood samples were taken once a week till the end of patients hospitalization, ending by patients discharge or death. Immunochemical identification of proteins under study were performed with Western-blotting technique. It was found that all proteins studied were present in both cerebrospinal fluid and blood serum on the first two days of the disease in small quantities. The blood content of both NSE and PV increased significantly during the first week of the disease. Both proteins disappeared from the blood serum between the second and fourth disease weeks. S-100 protein and GFAP contents in the blood reached significantly high level within the time interval between second and fifth disease weeks, and remained at a relatively high level till patients' death. In all cases computed tomography study and/or brain autopsy revealed extensive ischemic foci localized within areas supplied by the middle cerebral artery. No clear-cut correlation between extensiveness of the ischemic cerebral damage and the content of the proteins studied in both cerebrospinal fluid and blood serum was found. However, our data indicate that serial studies of the above proteins in patients with ischemic stroke may be useful in monitoring the progress of the disease, and occasionally in the prognosis at least in some cases.
Niebrój-Dobosz
I
I
Department of Neurology, School of Medicine, Polish Academy of Sciences, Warszawa.
Rafałowska
J
J
Lukasiuk
M
M
Pfeffer
A
A
Mossakowski
M J
MJ
D000368:Aged; D015153:Blotting, Western; D001921:Brain / Q000503:physiopathology; D002545:Brain Ischemia / Q000134:cerebrospinal fluid* / Q000000981:diagnostic imaging / Q000503:physiopathology; D005904:Glial Fibrillary Acidic Protein / Q000097:blood / Q000134:cerebrospinal fluid* / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D010320:Parvalbumins / Q000097:blood / Q000134:cerebrospinal fluid* / Q000276:immunology*; D010751:Phosphopyruvate Hydratase / Q000097:blood / Q000134:cerebrospinal fluid* / Q000276:immunology*; D014057:Tomography, X-Ray Computed
D005904:Glial Fibrillary Acidic Protein; D010320:Parvalbumins; D010751:Phosphopyruvate Hydratase