Anti-IgM and anti-IgG modulate c-fos RNA levels differently in human B lymphocytes.
One component of the B cell activation cascade is the induction of the protooncogene c-fos. Data presented here demonstrate that stimulation of mIgM-bearing cells with either anti-IgM or the combination of PDB plus ionomycin generated comparable levels of c-fos RNA. Furthermore, a synergistic response was observed when the cells were treated with selected concentrations of anti-IgM plus either PDB or ionomycin. In contrast, stimulation of mIgG-bearing B cells with anti-IgG did not induce the c-fos RNA levels that were seen when these cells were treated with the combination of PDB plus ionomycin. Treatment of mIgG-bearing cells with only the combination of anti-IgG plus ionomycin yielded a synergistic response and anti-IgG plus PDB did not. Thus, induction of c-fos RNA appears to be different in mIgM- and mIgG-bearing B cells after stimulation through mIg.
Shu
J
J
Department of Pharmacology and Toxicology, Higuchi Biosciences Center, University of Kansas, Lawrence 66045.
Benedict
S H
SH
Chan
M A
MA
D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology*; D015152:Blotting, Northern; D002460:Cell Line; D005786:Gene Expression Regulation / Q000276:immunology*; D016762:Genes, fos / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D015759:Ionomycin / Q000494:pharmacology; D009693:Nucleic Acid Hybridization / Q000276:immunology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012333:RNA, Messenger; D015240:Phorbol 12,13-Dibutyrate; D015759:Ionomycin