Cloning and high level expression of gene encoding ES antigen from Trichinella spiralis muscle larvae.
The partial structure gene encoding ES antigen derived from Trichinella spiralis (TSP) muscle larvae was cloned, characterized, and expressed in E. coli. The target DNA (0.7 kb) was directly obtained from the TSP total RNA by using RNA PCR technique. Based on the analysis with the RE digestion, the fragment was cloned into the fusion expression vector pEX31C. It was shown that a kind of 37kDa fusion protein was expressed in E. coli containing the recombinant plasmid by SDS-PAGE electrophoresis. The expressed protein was over 22% of the total cell protein, and it was aggregated in the form of inclusion bodies in E. coli. The purified protein could be recognized in ELISA both by sera from swine-infected with TSP and by the monoclonal antibody against TSP. These findings suggest that the recombinant protein is a potentially valuable antigen both for immunodiagnosis and vaccine development of trichinellosis.
Yan
Y
Y
Institute of Animal and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, China.
Xu
W
W
Chen
H
H
Ma
Z
Z
Zhu
Y
Y
Cai
S
S
D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis / Q000235:genetics*; D015153:Blotting, Western; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics; D015801:Helminth Proteins; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics; D017160:Trichinella spiralis / Q000235:genetics*
D000947:Antigens, Helminth; D015342:DNA Probes; D015801:Helminth Proteins; D011994:Recombinant Proteins; C045159:TES-26 protein, Toxocara canis