Circular dichroism studies of acid proteinases from Aspergillus niger and Aspergillus awamori.
Acid proteinases produced by strains of Aspergillus niger and Aspergillus awamori were isolated by means of ethanol precipitation, gel filtration and anion-exchange high resolution chromatography. In each case, the purified proteinase showed a single band in polyacrylamide gel electrophoresis. Their molecular weights were almost identical (approx. 45,000). However, the proteinase from Aspergillus awamori contained 16% of neutral hexoses while the other enzyme (Aspergillus niger) showed negligible amounts of these carbohydrates. Both enzymes displayed circular dichroism spectra that share a number of features with that of penicillopepsin. This suggests that proteinases from Aspergilli possess the structural folding pattern typical of aspartic proteinases. Proteolytic-activity pH optima were different, thus distinguishing one enzyme from another. This variation seems to be related to the particular resistance of the proteinases to acid denaturation, as indicated by changes in their circular dichroism spectra when the pH is decreased.
Tello-Solís
S R
SR
Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa.
Rodríguez-Romero
A
A
Hernández-Arana
A
A
D016282:Aspartic Acid Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification; D001230:Aspergillus / Q000201:enzymology*; D001234:Aspergillus niger / Q000201:enzymology; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D013045:Species Specificity
D002241:Carbohydrates; D005656:Fungal Proteins; D006023:Glycoproteins; D016282:Aspartic Acid Endopeptidases; C026442:Aspergillus acid proteinase