Replication of human cytomegalovirus in a rhabdomyosarcoma cell line depends on the state of differentiation of the cells.
The replication of human cytomegalovirus (HCMV) was investigated in a new human rhabdomyosarcoma cell line (KFR) with morphological and biochemical characteristics of fetal striated muscle precursors (rhabdomyoblasts). KFR cells exhibited the unique property for spontaneous morphological transformation from a poorly-differentiated state into well-differentiated (myotube-like) rhabdomyoblasts. The poorly-differentiated rhabdomyoblasts promoted both complete viral gene expression and the production of infectious virus. In contrast, in well-differentiated rhabdomyoblasts HCMV infection was abortive. The results showed that replication of HCMV in this human rhabdomyosarcoma cell line depended on the state of cellular differentiation.
Cinatl
J
J
Centre of Hygiene, Institute of Medical Virology, J. W. Goethe University, Frankfurt a. M, Federal Republic of Germany.
Cinatl
J
J
Radsak
K
K
Rabenau
H
H
Weber
B
B
Novak
M
M
Benda
R
R
Kornhuber
B
B
Doerr
H W
HW
D000956:Antigens, Viral / Q000096:biosynthesis; D001483:Base Sequence; D002454:Cell Differentiation; D003587:Cytomegalovirus / Q000276:immunology / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology / Q000821:virology*; D012208:Rhabdomyosarcoma / Q000473:pathology; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D014764:Viral Proteins; C044582:immediate-early proteins, cytomegalovirus