Ethanol neurotoxicity in vitro: effects of GM1 ganglioside and protein synthesis inhibition.
Cultures of septal and hippocampal neurons from fetal rat and dorsal root ganglion neurons from embryonic chick were pretreated with GM1 ganglioside or cycloheximide and then supplemented with toxic concentrations of ethanol. GM1 provided significant protection against ethanol neurotoxicity in each population. The inhibition of protein synthesis by cycloheximide, however, which protects against cell death resulting from withdrawal of neurotrophic factor support, did not ameliorate ethanol-induced neuronal loss.
Heaton
M B
MB
University of Florida Brain Institute, Department of Neuroscience, Gainesville 32610-0244.
Paiva
M
M
Swanson
D J
DJ
Walker
D W
DW
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003513:Cycloheximide / Q000494:pharmacology*; D000431:Ethanol / Q000633:toxicity*; D005333:Fetus; D005677:G(M1) Ganglioside / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009498:Neurotoxins / Q000633:toxicity*; D051381:Rats; D013997:Time Factors
D009419:Nerve Tissue Proteins; D009498:Neurotoxins; D005677:G(M1) Ganglioside; D000431:Ethanol; D003513:Cycloheximide