Growth inhibition of Trypanosoma cruzi and Leishmania donovani infantum by different snake venoms: preliminary identification of proteins from Cerastes cerastes venom which interact with the parasites.
Venom from three different snake species was tested in vitro against the protozoan parasites Trypanosoma cruzi and Leishmania donovani infantum. Two of them, Cerastes cerastes and Naja haje, exerted a significant growth inhibition of T. cruzi and L. d. infantum parasites. Heating of the venoms abolished their activity, suggesting that the active factors are thermolabile. Incubation of parasites with 125I-labelled C. cerastes venom proteins allowed preliminary identification of components which interact preferentially with the pathogens.
Fernandez-Gomez
R
R
Laboratoire de Recherche sur les Trypanosomatidae, INSERM U415, Institut Pasteur de Lille, France.
Zerrouk
H
H
Sebti
F
F
Loyens
M
M
Benslimane
A
A
Ouaissi
M A
MA
D000818:Animals; D001345:Autoradiography; D003470:Culture Media; D004546:Elapid Venoms / Q000737:chemistry / Q000494:pharmacology*; D017815:Elapidae; D004591:Electrophoresis, Polyacrylamide Gel; D007893:Leishmania donovani / Q000187:drug effects* / Q000254:growth & development; D011506:Proteins / Q000032:analysis*; D013696:Temperature; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development; D014757:Viper Venoms / Q000737:chemistry / Q000494:pharmacology*; D017819:Viperidae
D003470:Culture Media; D004546:Elapid Venoms; D011506:Proteins; D014757:Viper Venoms