title: Fragmentation characteristics of neuropeptides related to chromogranin B and proenkephalin B using fast atom bombardment and collision-induced dissociation.
abstract: This study deals with the mass spectral characterization of selected neuropeptides related to chromogranin B and proenkephalin B precursor proteins using fast atom bombardment (FAB) ionization in combination with low- and high-energy collision-induced dissociation. Fragmentation pathways were investigated using linked scan and tandem mass spectrometric techniques. First-order FAB mass spectra and product ion spectra of [M+H]+ ions are discussed and analysed for structure-specific information. In the high-energy product ion spectra, abundant y and c ions are found to be indicative of the presence of proline and threonine residues, respectively. With regard to side chain specific ions, diagnostic d and w ions are found, which support the presence of leucine, glutamic acid and glutamine at specific positions in the amino acid sequence.
authors.affiliation: University of Antwerp (UIA), Department of Pharmaceutical Sciences, Wilrijk, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Boel
authors.forename: L
authors.initials: L
authors.lastname: Dillen
authors.forename: H
authors.initials: H
authors.lastname: van den Heuvel
authors.forename: M
authors.initials: M
authors.lastname: Claeys
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D002864:Chromogranins / Q000737:chemistry*; D004745:Enkephalins / Q000737:chemistry*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry*; D011498:Protein Precursors / Q000737:chemistry*; D012697:Serine Endopeptidases / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D009626:Terminology as Topic; D014357:Trypsin
chemical_list: D000596:Amino Acids; D002864:Chromogranins; D004745:Enkephalins; D009479:Neuropeptides; D011498:Protein Precursors; C029992:proenkephalin; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin