Inhibitory effects of triazolodiazepine on mouse splenocytes and peritoneal macrophages in vitro.
Effects of triazolodiazepine (Tri) on mouse splenocyte proliferation and macrophage phagocytosis were studied. Tri 0.1-10 mumol.L-1 significantly inhibited concanavalin A (Con A) 5 micrograms.ml-1 and lipopolysaccharides (LPS) 10 micrograms.ml-1-induced splenocyte proliferation and phagocytic activity of activated peritoneal macrophages. Tri 10 mumol.L-1 reduced Con A-induced colony stimulating factor release from mouse splenocytes and LPS-challenged intracellular and extracellular interleukin-1 production from peritoneal macrophages. These results partially explain the wide and strong anti-inflammatory effects of Tri.
Ju
D W
DW
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China.
Zheng
Q Y
QY
Wang
H B
HB
Fang
J
J
D000818:Animals; D001381:Azepines / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D010587:Phagocytosis / Q000187:drug effects*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D013154:Spleen / Q000166:cytology* / Q000378:metabolism; D014230:Triazoles / Q000494:pharmacology*
D001381:Azepines; D003115:Colony-Stimulating Factors; D007375:Interleukin-1; D010972:Platelet Activating Factor; D014230:Triazoles; C052518:WEB 2086