title: Nucleotide and deduced amino acid sequence of human cysteinyl-tRNA synthetase.
abstract: We have determined the nucleotide sequence of a cDNA coding human cysteinyl-tRNA synthetase. The predicted protein sequence of 638 amino acids has substantial sequence similarity to the E. coli cysteinyl-tRNA synthetase in the nucleotide-binding fold region that constitutes the potential active site. The results are consistent with the hypothesis that evolution of the full complement of aminoacyl-tRNA synthetases occurred prior to the divergence of prokaryotes and eukaryotes.
authors.affiliation: Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717.
authors.forename: M E
authors.initials: ME
authors.lastname: Cruzen
authors.forename: S M
authors.initials: SM
authors.lastname: Arfin
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D018076:DNA, Complementary; D000604:Amino Acyl-tRNA Synthetases; C019975:cysteinyl-tRNA synthetase