[Effect of antiseptics and redox-cycling agents on Corynebacterium ammoniagenes and related microorganisms in relation to synthesis of a new macroergic compound].
Sublethal concentration of the antiseptic composition Desoxon-1 was shown to provoke in cells of Corinebacterium ammoniagenes in a liquid medium the biosynthesis and accumulation of a novel macroergic 2-methylbutane-1,2,3,4-tetraol-2,4-cyclopyrophosphate. This substance is also synthesized when C. ammoniagenes is cultivated in a solid agar medium supplemented with benzylviologen. Cells preloaded with the new cyclopyrophosphate maintain its content when treated with 4% phenol, DP-2, Desoxon-1 or boiled and heated in an autoclave. Experiments with Mycobacterium tuberculosis and BCG revealed the ability of these bacteria to grow in a medium supplemented with BV++ possibly due to ability of synthesis of a new cyclopyrophosphate which was shown to correlate with resistance toward redox-cycling drugs. Accumulation of polyphosphates in the control cells of M. tuberculosis was illustrated by 31P-NMR spectroscopy and disappearance of the polyphosphates during cultivation in a BV(++)-supplemented medium. No signal of the new cyclopyrophosphate was yet registered in cells of M. tuberculosis by 31P-NMR.
Ostrovskiĭ
D N
DN
Dosanov
K Sh
KSh
Kaliuk
A N
AN
Ogrel'
O D
OD
Sibel'dina
L A
LA
Kharat'ian
E F
EF
Shchipanova
I N
IN
Sharov
A N
AN
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001951:Brevibacterium / Q000187:drug effects* / Q000378:metabolism; D003352:Corynebacterium / Q000187:drug effects* / Q000378:metabolism; D004896:Erythritol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005609:Free Radicals; D009682:Magnetic Resonance Spectroscopy; D009163:Mycobacterium bovis / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010463:Peracetic Acid; D013481:Superoxides / Q000378:metabolism
D000891:Anti-Infective Agents, Local; D005609:Free Radicals; D013481:Superoxides; C073443:2-methyl-butan-1,2,3,4-tetraol-2,4-cyclopyrophosphate; D010463:Peracetic Acid; D004896:Erythritol
Deĭstvie antisepticheskikh sredstv i redokstsikliruiushchikh agentov na Corynebacterium ammoniagenes i rodstvennye mikroorganizmy v sviazi s sintezom novogo makroérga.