title: Biochemical characterization of glucosaminylmuramyldipeptide binding sites of murine macrophages.
abstract: By using radioligand analysis, murine peritoneal macrophages were shown to express several hundred high-affinity cell surface GMDP-binding sites (Ka 350 pM). Photoaffinity labeling followed by SDS-PAGE enabled us to identify 32-34 and 38 kDa proteins inside these cells that bound GMDP specifically.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: T N
authors.initials: TN
authors.lastname: Golovina
authors.forename: M V
authors.initials: MV
authors.lastname: Sumaroka
authors.forename: L V
authors.initials: LV
authors.lastname: Samokhvalova
authors.lastname: Shebzukhov YuV
authors.forename: T M
authors.initials: TM
authors.lastname: Andronova
authors.forename: V A
authors.initials: VA
authors.lastname: Nesmeyanov
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000737:chemistry; D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002240:Carbohydrate Sequence; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011869:Radioligand Assay
chemical_list: D000276:Adjuvants, Immunologic; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C063429:glucosaminylmuramyl-2-alanine-D-isoglutamine