[Affinity modification of EcoRII restriction endonuclease by a DNA-duplex containing a monosubstituted pyrophosphate internucleotide bond].
Oligonucleotide duplex with an active monosubstituted pyrophosphate bond within the recognition site of the EcoRII restriction endonuclease was cross-linked to this enzyme with a yield of 10-15%. The cross-linking specificity was proved by the absence of the cross-linking to a DNA duplex with the same modification but without the EcoRII recognition site as well as by unmodified EcoRII substrate's inhibition of the cross-linking.
Shabarova
Z A
ZA
Sheflian
G Ia
GIa
Kuznetsova
S A
SA
Kubareva
E A
EA
Sysoev
O N
ON
Ivanovskaia
M G
MG
Gromova
E S
ES
D000345:Affinity Labels; D001483:Base Sequence; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry*; D011756:Diphosphates / Q000032:analysis*; D008969:Molecular Sequence Data
D000345:Affinity Labels; D003432:Cross-Linking Reagents; D011756:Diphosphates; D004247:DNA; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Affinnaia modifikatsiia éndonukleazy restriktsii EcoRII DNA-dupleksom, soderzhashchim monozameshchennuiu pirofosfatnuiu mezhnukleotidnuiu sviaz'.