Control of kinetic properties of AMPA receptor channels by nuclear RNA editing.
AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptor channels mediate the fast component of excitatory postsynaptic currents in the central nervous system. Site-selective nuclear RNA editing controls the calcium permeability of these channels, and RNA editing at a second site is shown here to affect the kinetic aspects of these channels in rat brain. In three of the four AMPA receptor subunits (GluR-B, -C, and -D), intronic elements determine a codon switch (AGA, arginine, to GGA, glycine) in the primary transcripts in a position termed the R/G site, which immediately precedes the alternatively spliced modules "flip" and "flop." The extent of editing at this site progresses with brain development in a manner specific for subunit and splice form, and edited channels possess faster recovery rates from desensitization.
Lomeli
H
H
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Germany.
Mosbacher
J
J
Melcher
T
T
Höger
T
T
Geiger
J R
JR
Kuner
T
T
Monyer
H
H
Higuchi
M
M
Bach
A
A
Seeburg
P H
PH
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D005091:Exons; D018698:Glutamic Acid / Q000494:pharmacology; D005998:Glycine / Q000235:genetics; D007438:Introns; D007700:Kinetics; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D009865:Oocytes; D016716:PC12 Cells; D018408:Patch-Clamp Techniques; D017393:RNA Editing; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014981:Xenopus
D018091:Receptors, AMPA; D011994:Recombinant Proteins; D018698:Glutamic Acid; D005998:Glycine