Magnetically responsive diclofenac sodium-loaded erythrocytes: preparation and in vitro characterization.
Diclofenac sodium-bearing magnetic erythrocytes were prepared using a preswell technique. The optimum loading of drug and magnetite achieved were 63-73 and 12-18 per cent, respectively. Drug-loaded erythrocytes and drug-loaded magnetic erythrocytes were characterized for in vitro drug and haemoglobin release, magnetic responsiveness, osmotic fragility, turbulence shock, morphology and percentage cell recovery. The drug-loaded magnetic erythrocytes were found less resistant to osmotic and turbulence shock when compared with the normal and drug-loaded erythrocytes. However, in optimum concentration erythrocytes tolerated drug and coated magnetite appreciably. The drug-loaded magnetic erythrocytes responded effectively for an external magnetic field of 8.0 kOe. The study suggested the potentiality of diclofenac sodium-loaded magnetic erythrocytes, for active delivery of drug to painful inflamed joints.
Jain
S K
SK
Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Sagar, (MP), India.
Vyas
S P
SP
D000818:Animals; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D004008:Diclofenac / Q000008:administration & dosage*; D004337:Drug Carriers / Q000737:chemistry; D004912:Erythrocytes / Q000737:chemistry* / Q000502:physiology; D052203:Ferrosoferric Oxide; D006454:Hemoglobins / Q000378:metabolism; D007501:Iron; D008280:Magnetics; D008297:Male; D009996:Osmotic Fragility; D010087:Oxides; D051381:Rats
D004337:Drug Carriers; D006454:Hemoglobins; D010087:Oxides; D004008:Diclofenac; D007501:Iron; D052203:Ferrosoferric Oxide