title: Quantitative analysis of residual detergent in proteoliposomes by Fourier transform infrared spectroscopy.
abstract: An infrared spectroscopic method has been developed to quantify the residual amount of detergent present in membranes, e.g., after reconstitution of a membrane protein in phospholipids. A detergent-specific band is selected in the ir spectrum and the intensity is ratioed against a phospholipid-specific band (around 1235 cm-1, vas (P = O)). This ratio shows a linear relation with the molar ratio of detergent of phospholipid, down to as low as 1 to 10. The new method is illustrated in two case studies, viz., tris(hydroxyethyl)ammonium cholate in Na+/K(+)-ATPase proteoliposomes and n-dodecyl-beta-D-maltoside in rhodopsin proteoliposomes.
authors.affiliation: Department of Biochemistry, University of Nijmegen, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Pistorius
authors.forename: F M
authors.initials: FM
authors.lastname: Stekhoven
authors.forename: P H
authors.initials: PH
authors.lastname: Bovee-Geurts
authors.forename: W J
authors.initials: WJ
authors.lastname: de Grip
mesh_terms: D000818:Animals; D002138:Calibration; D002417:Cattle; D003902:Detergents / Q000032:analysis*; D011510:Proteolipids / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003902:Detergents; D011510:Proteolipids; C020485:proteoliposomes