Disappearance of heme metabolites following chelation therapy with meso 2,3-dimercaptosuccinic acid (DMSA).
A lead poisoned adult (blood lead level 384 micrograms/dL) had a specific urinary porphyrin profile of elevated porphyrins including coproporphyrin III and 5-carboxylic acid derivatives. A multilinear gradient elution, modified with an ion-pair and a reversed phase column were employed for the separation of the diagnostically important porphyrin isomers. Fluorescence detection enhanced both sensitivity and selectivity. Both compounds were restored to normal levels following two courses of meso 2,3-dimercaptosuccinic acid: 90 mg/kg/d x 5 d at one month intervals. The decrease of clinical symptoms was associated with increase of delta aminolevulinic acid dehydratase from 53 to 230 U/mL blood and hemoglobin from 8.4 to 12.7 g/dL. Blood lead decreased from 384 to 24 micrograms/dL, urine lead from 1286 to 188 micrograms/L and urine coproporphyrin III from 5712 to 25 micrograms/L.
Tutunji
M F
MF
Department of Chemistry, Faculty of Science, University of Jordan, Amman.
al-Mahasneh
Q M
QM
D000284:Administration, Oral; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003306:Coproporphyrins / Q000652:urine; D006801:Humans; D007855:Lead Poisoning / Q000097:blood / Q000188:drug therapy* / Q000652:urine; D008297:Male; D008669:Metallurgy; D009784:Occupational Diseases / Q000097:blood / Q000188:drug therapy* / Q000652:urine; D016273:Occupational Exposure; D000623:Porphobilinogen Synthase / Q000097:blood; D004113:Succimer / Q000627:therapeutic use*
D003306:Coproporphyrins; C026971:coproporphyrin III; D004113:Succimer; D000623:Porphobilinogen Synthase