[Production and identification of a monoclonal antibody against a tumor associated antigen from ovarian epithelial carcinoma].
The splenocyte of BALB/c mice, immunized with soluble and ovarian serous papillary cystedenoma-associated antigen CA925, was fused with NS-1 myeloma cell strain to produce a monoclonal antibody. 5 cell strains of the monoclonal antibody with continuous secretion were obtained. The fusion openings were used as code names: OC4D9, OC7E10, OC1B4, OC3B8, and OC9B9, their titers were 1: 10(7), 1: 10(7), 1: 10(6), 1: 10(4) and 1: 10(5) respectively. Ig subgroups were IgM and IgG. The chromosomes showed the characteristics of their parents. The 5 strains of the monoclonal antibody obtained presented specific reactions to CA925, no positive reaction with normal ovarian tissues or leiomyoma of the uterus were seen, and there were weak reactions with carcinomas of the lung, the stomach, the kidney, the esophagus and the rectum. Immunohistochemical tests proved that the monoclonal antibody produced was specific against ovarian epithelial carcinoma, and it might offer a means to the diagnosis and treatment of malignant ovarian tumors.
Gong
G L
GL
Third Clinical College, Norman Bethune University of Medical Sciences, Changchun.
Zou
J Y
JY
Cai
Z T
ZT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D018283:Cystadenocarcinoma, Papillary / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010051:Ovarian Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate