Identification of metabolic intermediates in microbial degradation of phenol using laser desorption time-of-flight mass spectrometry.
Laser desorption time-of-flight (LD-TOF) mass spectrometry was utilized to determine the metabolic intermediates in microbial degradation of phenol. The identified components were in good agreement with the well-documented pathway. This technique also demonstrated excellent precision. Analytical merits, instrumentation and methodology are discussed.
Xu
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Department of Chemistry, University of Kentucky, Lexington 40506.
Majidi
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V
D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D004785:Environmental Pollutants / Q000378:metabolism*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D019800:Phenol; D010636:Phenols / Q000378:metabolism*; D016958:Pseudomonas putida / Q000378:metabolism*
D004785:Environmental Pollutants; D010636:Phenols; D019800:Phenol