Similarity between pyridoxal/pyridoxamine phosphate-dependent enzymes involved in dideoxy and deoxyaminosugar biosynthesis and other pyridoxal phosphate enzymes.
A multiple sequence alignment among aspartate aminotransferase, dialkylglycine decarboxylase, and serine hydroxymethyltransferase (DAS) was used for profile databank search. The DAS profile could detect similarities to other pyridoxal or pyridoxamine phosphate-dependent enzymes, like several gene products involved in dideoxysugar and deoxyaminosugar synthesis. The alignment among DAS and such gene products shows the conservation of aspartate 222 and lysine 258, which, in aspartate aminotransferase, interacts with the N1 of the coenzyme pyridine ring and forms the internal Schiff base, respectively. The lysine is replaced by histidine in the pyridoxamine phosphate-dependent gene products. The alignment indicates also that the region encompassing the coenzyme binding site is the most conserved.
Pascarella
S
S
Dipartimento di Scienze Biochimiche, Università La Sapienza, Rome, Italy.
Bossa
F
F
D000595:Amino Acid Sequence; D001219:Aspartate Aminotransferases / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D002262:Carboxy-Lyases / Q000737:chemistry* / Q000378:metabolism; D016208:Databases, Factual; D012696:Glycine Hydroxymethyltransferase / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011732:Pyridoxal Phosphate / Q000378:metabolism / Q000494:pharmacology*; D011733:Pyridoxamine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D016415:Sequence Alignment; D012984:Software
D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D012696:Glycine Hydroxymethyltransferase; D001219:Aspartate Aminotransferases; D002262:Carboxy-Lyases; C063519:2,2-dialkylglycine decarboxylase; C010627:pyridoxamine phosphate