Rapid detection of rotavirus in faeces using a dipstick system with monoclonal antibodies and colloidal gold as marker.
Rotavirus (RV) is known to be the most common cause of severe diarrhoea in infants and young children, each year leading to an estimated 800,000-900,000 deaths. RV also infects bovines and other species, with high morbidity and mortality. A rapid and simple 'naked-eye' dipstick system was developed to detect human RV in faeces, using nitrocellulose as solid phase, two monoclonal antibodies, and colloidal gold as marker. The system detects 10(4) viral particles (1-2 ng)/g of faeces. For human RV the specificity and sensitivity were 100% when compared with a commercial latex system, and 99% and 98%, respectively, when correlated with traditional RNA-PAGE, and 100% and 98% compared to an ELISA system.
Fernández
D
D
Division of Immunotechnology and Diagnostics, Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.
Valle
I
I
Llamos
R
R
Guerra
M
M
Sorell
L
L
Gavilondo
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology*; D005759:Gastroenteritis / Q000175:diagnosis / Q000821:virology; D017979:Gold Colloid; D006801:Humans; D007223:Infant; D051379:Mice; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000821:virology; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D017979:Gold Colloid