Interleukin-1 beta induces cytosolic phospholipase A2 gene in rats C6 glioma cell line.
Treatment of the rat C6 glioma cell line with interleukin-1 beta (IL-1 beta) resulted in an accumulation of cytosolic phospholipase A2 (cPLA2) in mRNA level. The increase of cPLA2 in mRNA level was observed at 6 hours after treatment of IL-1 beta and reached to the maximal level at 12 hours. The accumulation also remained sustained for up to 72 hours. Dose response curve of IL-1 beta showed that as little as 40 units/ml concentration induced the accumulation of mRNA and 100 units/ml concentration showed the maximal effect. In contrast, cyclooxygenase 2 gene was not affected with treatment of IL-1 beta.
Ozaki
M
M
Department of Neuroscience, Osaka Bioscience Institute, Japan.
Morii
H
H
Qvist
R
R
Watanabe
Y
Y
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic; D005910:Glioma; D007375:Interleukin-1 / Q000494:pharmacology*; D010741:Phospholipases A / Q000235:genetics* / Q000378:metabolism; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D012333:RNA, Messenger; D010741:Phospholipases A; D054467:Phospholipases A2