Synergistic effect of oridonin and cisplatin on cytotoxicity and DNA cross-link against mouse sarcoma S180 cells in culture.
Oridonin (Ori) is an active principle isolated from Rabdosia rubescens. The cytotoxic effect of the combination of Ori and cisplatin was tested by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay. IC50 of cisplatin to cultured S180 cells in 24 h was 9.38 micrograms.ml-1. When the cells were treated with cisplatin plus Ori 0.5 and 1 microgram.ml-1, the IC50 were 1/3.4 and 1/6.7, respectively, of that with cisplatin alone. Modified alkaline elution was used to detect the DNA interstrand cross-link and DNA-protein cross-link in S180 cells induced by the 2 drugs. A greater amount of DNA cross-link was detected when the cells were treated with cisplatin plus Ori than with cisplatin alone (P < 0.05). After lysis by proteinase K, a reduction in DNA cross-link was seen, which suggested that the drugs could produce both kinds of DNA cross-link.
Gao
Z G
ZG
Department of Pharmacology, He-nan Institute of Medical Sciences, Zhengzhou, China.
Ye
Q X
QX
Zhang
T M
TM
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D045786:Diterpenes, Kaurane; D004357:Drug Synergism; D051379:Mice; D012510:Sarcoma 180 / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D004273:DNA, Neoplasm; D004224:Diterpenes; D045786:Diterpenes, Kaurane; C011959:oridonin; D002945:Cisplatin