Estimation of xyloglucan in vegetables by enzyme-linked immunosorbent assay.
We prepared the antibody specific against plant xyloglucan and applied it to enzyme-linked immunosorbent assay in order to estimate xyloglucan contents in several vegetables. In this experiment, xyloglucan was detected in the 24% aqueous potassium hydroxide-soluble fractions of tomato, cabbage, lettuce, and eggplant. The xyloglucan contents of these vegetables were estimated to be 1.4 mg, 54 micrograms, 14 micrograms, and 4 micrograms per one hundred grams of their edible portions, respectively, using tamarind xyloglucan as a standard xyloglucan.
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Department of Food and Nutrition, Faculty of Science of Living, Osaka City University, Japan.
Fujikawa
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D005591:Chemical Fractionation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005936:Glucans; D008745:Methylation; D011134:Polysaccharides / Q000032:analysis* / Q000276:immunology; D014675:Vegetables / Q000737:chemistry*; D014990:Xylans
D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan