title: Effects of carbamylcholine chloride on human antral gastrin mRNA levels.
abstract: The effects of the muscarinic receptor agonist, carbamylcholine chloride (carbachol), on gastrin release and gastrin mRNA levels in human antral mucosa (n = 15) were determined. During a-2-h incubation period, carbachol (10(-6)-10(-4) M) decreased gastrin mRNA levels to 71 +/- 8% (10(-6) M), 40 +/- 8% (10(-5) M), and 33 +/- 5% (10(-4) M) of control levels. Carbachol (10(-5) M) decreased intracellular gastrin (from 1634 +/- 103 to 1272 +/- 126 pg/mg tissue protein), while it increased gastrin release into the medium (from 609 +/- 48 to 918 +/- 68 pg/ml per mg tissue protein). After 6- and 9-h culture, carbachol gradually increased gastrin mRNA levels, by 96 +/- 12% and 126 +/- 23%, respectively. Atropine sulfate (10(-5) M) completely inhibited the carbachol-induced changes. Cycloheximide markedly decreased tissue gastrin concentration, but increased gastrin mRNA levels, whereas it had no effects on gastrin release. These findings suggested that carbachol may have a time-related biphasic action on human antral gastrin biosynthesis.
authors.affiliation: First Department of Internal Medicine, Kochi Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Okazaki
authors.forename: J
authors.initials: J
authors.lastname: Kino
authors.forename: K
authors.initials: K
authors.lastname: Suenaga
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D000328:Adult; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology*; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D005755:Gastrins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D011706:Pyloric Antrum / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D005755:Gastrins; D012333:RNA, Messenger; D001285:Atropine; D002217:Carbachol; D003513:Cycloheximide