Characterization of a novel heteroglucan from the rhizome of Cnidium officinale exhibiting high reticuloendothelial system-potentiating and anti-complementary activities.
A novel beta-heteroglucan, called cnidirhan SIIA, was isolated from the rhizome of Cnidium officinale MAKINO. It was homogeneous on electrophoresis and gel chromatography, and its molecular mass was more than 1 x 10(7). It was composed of D-glucose, D-galactose and L-arabinose in the molar ratio of 85:5:8, in addition to small numbers of O-acetyl groups. Methylation analysis and nuclear magnetic resonance studies indicated its main structural features composed of beta-1,6-linked D-glucopyranosyl residues with both 3,6- and 4,6-branching points. In addition, it has both beta-1,4-linked L-arabinopyranosyl and beta-1,6-linked D-galactopyranosyl residues. The polysaccharide showed very pronounced reticuloendothelial system-potentiating activity in a carbon clearance test, as well as a marked anti-complementary activity.
Tomoda
M
M
Kyoritsu College of Pharmacy, Tokyo, Japan.
Ohara
N
N
Shimizu
N
N
Gonda
R
R
D000328:Adult; D000818:Animals; D003169:Complement Inactivator Proteins / Q000494:pharmacology*; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012157:Mononuclear Phagocyte System / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry*
D003169:Complement Inactivator Proteins; D005936:Glucans