Expression of prelamin A but not mature lamin A confers sensitivity of DNA biosynthesis to lovastatin on F9 teratocarcinoma cells.
The role of inhibition of prelamin A processing in the inhibition of DNA synthesis by lovastatin was examined by expressing prelamin A in F9 teratocarcinoma cells. These cells, normally lacking expression of the A/C lamins, were transfected with constructs expressing either prelamin A or mature lamin A and the effect of lovastatin on DNA biosynthesis was assessed. It was found that expression of prelamin A specifically conferred sensitivity to inhibition of DNA biosynthesis by lovastatin on F9 cells.
Sinensky
M
M
Eleanor Roosevelt Institute, Denver, CO 80206.
McLain
T
T
Fantle
K
K
D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D034904:Lamin Type A; D034882:Lamins; D008148:Lovastatin / Q000494:pharmacology*; D008798:Mevalonic Acid / Q000378:metabolism; D051379:Mice; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D011499:Protein Processing, Post-Translational; D016283:Proto-Oncogene Proteins p21(ras) / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis; D013234:Stem Cells; D018243:Teratocarcinoma; D014162:Transfection; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; D011498:Protein Precursors; D011994:Recombinant Proteins; C090764:prelamin A; D003907:Dexamethasone; D002784:Cholesterol; D008148:Lovastatin; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); D008798:Mevalonic Acid