C3-independent glomerulonephritis in guinea pigs: dependence upon primary humoral response.
We used C3-deficient (C3D) guinea pigs to evaluate the role of C3 in an active model of experimental nephritis. Normal strain 2 (C3N, n = 13) and C3D (n = 6) guinea pigs were immunized with cationized bovine gamma-globulin (CBGG). Fourteen days later (Day 0), daily intravenous injections of CBGG were given for 3 to 7 days and the animals were sacrificed on Day 10 or 21. Immunofluorescence (IF) microscopy of renal tissue revealed two patterns of glomerular IgG deposition: granular loop (11/13 C3N, 3/6 C3D), and predominantly mesangial (2/13 C3N, 3/6 C3D). Codeposited C3 was seen in all C3N and in no C3D animals. Electron microscopy showed subepithelial deposits in all. A significant correlation (P < 0.005) was seen between an animal's IF pattern and its level of serum antibodies to CBGG; those with lower antibody levels exhibited the mesangial pattern. C3D animals had lower mean antibody levels than C3N (P < 0.01), but both IF patterns were represented. Urine protein concentration, which was increased relative to controls, did not differ between C3N and C3D groups, but was significantly greater in those with loop IF. Serum albumin was significantly reduced in animals with loop IF. C3N animals showed a significant reduction in mean serum C3. In this model, immune deposit location and degree of proteinuria are independent of C3 deposition and dependent upon the level of antibody response to CBGG. Induction of antibody to CBGG is impaired in the absence of C3.
Schurman
S J
SJ
Division of Nephrology, Children's Hospital Medical Center, Cincinnati, Ohio 45229-3039.
McAdams
A J
AJ
Beischel
L
L
Davis
A E
AE
Welch
T R
TR
D000818:Animals; D000906:Antibodies / Q000097:blood; D000917:Antibody Formation; D003167:Complement Activation; D003176:Complement C3 / Q000032:analysis / Q000502:physiology*; D005921:Glomerulonephritis / Q000276:immunology*; D006168:Guinea Pigs; D006417:Hematuria / Q000378:metabolism; D007105:Immune Complex Diseases / Q000276:immunology; D008853:Microscopy; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011507:Proteinuria / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism
D000906:Antibodies; D003176:Complement C3; D012709:Serum Albumin