[Studies on the relationship between HPV and respiratory tract tumors. I. Correlation between HPV and laryngeal carcinoma].
In this paper, alpha-32P-dCTP-labeled HPV-11 and HPV-16 DNA were used as probe, and slot and Southern blot hybridization techniques were used to detect HPV-11 and HPV-16 DNA related sequences in 37 laryngeal carcinoma tissues taken from patients in Qingdao and Beijing. Using the slot blot technique, 86% (32/37) of the cases showed positive hybridization with HPV-11 probe, while 81% (30/37) showed positive results with HPV-16. Another 81% (30/37) showed positive hybridization with HPV-11 and HPV-16 simultaneously. Using Southern blot hybridization Pst digestion, the hybridization pattern corresponded to that of HPV-16 in 46; (13/28), and none corresponded to HPV-11. The results suggest that HPV-16 may induce laryngeal carcinoma.
Cui
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Cancer Institute, CAMS and PUMC, Beijing.
Yang
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He
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D000328:Adult; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000821:virology*; D015344:DNA Probes, HPV; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000821:virology*; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections; D014412:Tumor Virus Infections
D015344:DNA Probes, HPV; D004273:DNA, Neoplasm