[Effect of gonadotropin-releasing peptide (GRP) on LH secretion of mouse pituitary in vitro].
Synthetic GRP and its analogues, GRP-NH2, [Glu7.9.14 Lys6.10] GRP (6-14), [Phe14] GRP (5-14) and [Phe14] GRP, at the concentration of 0.05 mmol.L-1 were shown to have stimulatory effect on LH secretion in cultured mouse pituitary in vitro. The luteotropin releasing activity of GRP and its analogues was estimated to be 115.4, 114.2, 140.0, 162.0 and 179.0% of the control group, respectively. Subcutaneous administration of [Phe14] GRP on days 7-9 and 1-5 of pregnancy and [Phe14] GRP (5-14) on days 2-4 of gestation at dosage of 1 mg.d-1 (each mouse) caused fetal death in 40-60% of mice.
Wang
N G
NG
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical University, Beijing.
Guan
M Z
MZ
Wang
D X
DX
Lu
G S
GS
D000019:Abortifacient Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D000028:Abortion, Induced; D000818:Animals; D010064:Embryo Implantation / Q000187:drug effects; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000138:chemical synthesis / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009924:Organ Culture Techniques; D010902:Pituitary Gland / Q000378:metabolism*; D011247:Pregnancy
D000019:Abortifacient Agents; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone