title: Virus-like particles in MDCK cells persistently infected with Borna disease virus.
abstract: A line of Madin Darby canine kidney (MDCK) cells persistently infected with Borna disease virus was examined by electron microscopy. Thin sections revealed the presence of intracytoplasmic virus-like particles ranging from 50-100 nm in diameter. Nuclei of the infected cells exhibited accumulation of electron-dense granular structures 15-18 nm in diameter. The intracytoplasmic particles were roughly spherical with a limiting membrane, suggesting the presence of a lipid-containing envelope. The internal structure consisted of strand-like material which in some cases was condensed underneath the envelope. The possible relationship of these particles to Borna disease virions is discussed.
authors.affiliation: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322.
authors.forename: R W
authors.initials: RW
authors.lastname: Compans
authors.forename: L R
authors.initials: LR
authors.lastname: Melsen
authors.forename: J C
authors.initials: JC
authors.lastname: de la Torre
mesh_terms: D000818:Animals; D001891:Borna disease virus / Q000254:growth & development / Q000648:ultrastructure*; D002460:Cell Line; D002467:Cell Nucleus / Q000821:virology*; D003593:Cytoplasm / Q000821:virology*; D004285:Dogs; D004847:Epithelial Cells; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure*; D007668:Kidney; D008854:Microscopy, Electron; D014771:Virion / Q000648:ultrastructure