title: A closed chamber for intravital microscopy of the rat mesentery under controlled oxygen tension and temperature.
abstract: An airtight and watertight closed chamber for intravital microscopy of the rat mesentery was developed. It incorporates an oxygen microelectrode and a thermistor for monitoring the local environment around the mesentery, as well as a heater for controlling the chamber temperature. This chamber provides an alternative to the superfusion method for intravital microscopy of the mesenteric microcirculation under circumstances where well-defined experimental conditions are required.
authors.affiliation: Department of Physiology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Yaegashi
authors.forename: T
authors.initials: T
authors.lastname: Kosaka
authors.forename: T
authors.initials: T
authors.lastname: Kinoshita
authors.forename: T
authors.initials: T
authors.lastname: Morimoto
mesh_terms: D000818:Animals; D004867:Equipment Design; D008297:Male; D008643:Mesentery / Q000033:anatomy & histology* / Q000502:physiology*; D008833:Microcirculation; D008853:Microscopy / Q000295:instrumentation*; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation; D013696:Temperature
chemical_list: D010100:Oxygen