Molecular cloning of human cytosolic purine 5'-nucleotidase.
The cDNA of human cytosolic purine 5'-nucleotidase (EC 3.1.3.5), which is the supposed regulatory allosteric enzyme of purine nucleotide degradation, has been cloned from a human placenta cDNA library. The predicted open reading fram encodes a protein of 561 amino acids with a molecular mass of 64,966 Da. The deduced amino acid sequence exhibits 95% identity with the sequence of the B type subunit of chicken cytosolic purine 5'-nucleotidase. Northern blot analysis of human placenta poly(A) +RNA revealed a single band of 3.6 kilobases using the cloned cDNA.
Oka
J
J
National Institute of Health and Nutrition, Tokyo, Japan.
Matsumoto
A
A
Hosokawa
Y
Y
Inoue
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003600:Cytosol / Q000201:enzymology*; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D009708:Nucleotidases / Q000235:genetics*
D018076:DNA, Complementary; D009708:Nucleotidases; C029296:purine 5'-nucleotidase