Cloning and sequence analysis of the gene encoding pyruvate kinase in Trypanoplasma borelli.
The gene coding for pyruvate kinase in Trypanoplasma borelli has been cloned and characterized. A single gene copy was found with an open reading frame for a polypeptide of 496 amino acids and a molecular mass of 54337. The deduced amino acid sequence has a calculated net charge of -3. Comparison of the sequence with those of pyruvate kinases from members of the family Trypanosomatidae revealed amino acid identities of 58.4-61.5%, and, to some extent, conservation of residues supposed to be involved in the binding of the allosteric effector fructose 2,6-bisphosphate. Some kinetic properties of Trypanoplasma borelli pyruvate kinase have been determined and appear to be similar to those of the enzyme from Trypanosoma brucei and Leishmania mexicana.
Ernest
I
I
Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Opperdoes
F R
FR
Michels
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000032:analysis; D017125:Genes, Protozoan; D007700:Kinetics; D016830:Kinetoplastida / Q000201:enzymology* / Q000235:genetics*; D007894:Leishmania mexicana / Q000201:enzymology; D008969:Molecular Sequence Data; D011770:Pyruvate Kinase / Q000235:genetics* / Q000378:metabolism; D016053:RNA, Protozoan / Q000032:analysis; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014346:Trypanosoma brucei brucei / Q000201:enzymology
D016054:DNA, Protozoan; D016053:RNA, Protozoan; D011770:Pyruvate Kinase