title: Immunohistochemical study of cathepsin B. Prognostic significance in human lung cancer.
abstract: BACKGROUND
  The concentration of cysteine proteinase cathepsin B has been shown to be elevated in association with malignancy or metastatic potential of human and rodent tumors, but its prognostic value for human lung cancer remains undetermined.
  
  
  METHODS
  Using a polyclonal antibody, immunohistochemical analyses of cathepsin B were performed on paraffin embedded sections of tumors obtained surgically from 108 patients with non-small cell lung cancer (49 squamous cell carcinomas, 59 adenocarcinomas). The immunohistochemical expressions of cathepsin B in the tumors were compared with patient survival.
  
  
  RESULTS
  Higher grade expression of cathepsin B was associated significantly with shorter survival in non-small cell lung cancer (P < 0.01), in squamous cell carcinoma (P < 0.05), and in adenocarcinoma (P < 0.01). A similar result also was seen in Stage I non-small cell lung cancer (P < 0.05).
  
  
  CONCLUSIONS
  The authors concluded that the immunohistochemical staining pattern of cathepsin B may be a useful predictor of survival for human lung cancer.
authors.affiliation: First Department of Medicine, School of Medicine, Hokkaido University Hospital, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sukoh
authors.forename: S
authors.initials: S
authors.lastname: Abe
authors.forename: S
authors.initials: S
authors.lastname: Ogura
authors.forename: H
authors.initials: H
authors.lastname: Isobe
authors.forename: H
authors.initials: H
authors.lastname: Takekawa
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: Y
authors.initials: Y
authors.lastname: Kawakami
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000201:enzymology / Q000401:mortality; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis; D002401:Cathepsin B / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000201:enzymology / Q000401:mortality; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011237:Predictive Value of Tests; D011379:Prognosis; D012044:Regression Analysis; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D002401:Cathepsin B