Physical interaction of the HIV-1 Nef protein with beta-COP, a component of non-clathrin-coated vesicles essential for membrane traffic.
Nef is a 27-kDa myristylated protein conserved in most human immunodeficiency virus (HIV)-1, HIV-2, and simian immunodeficiency virus isolates. Simian immunodeficiency virus Nef is required in macaques for both high viral load and full pathological effects. Nef down-regulates the cell surface expression of CD4 by a post-translational mechanism that is not yet fully elucidated. We have used the yeast two-hybrid system to identify cellular proteins that interact with Nef. A cDNA was isolated which encodes a COOH-terminal fragment of human beta-COP, a major coat component of non-clathrin-coated vesicles. Nef and beta-COP interacted in vitro and were found to be physically associated in HIV-1-infected cells by co-immunoprecipitation. These observations suggest that beta-COP might be one of the cellular mediators of Nef function in HIV-1-infected cells.
Benichou
S
S
Unité 332, INSERM, Paris, France.
Bomsel
M
M
Bodéus
M
M
Durand
H
H
Douté
M
M
Letourneur
F
F
Camonis
J
J
Benarous
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism*; D020756:Coatomer Protein; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000302:isolation & purification; D015693:Gene Products, nef / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D015498:HIV-2 / Q000378:metabolism; D006801:Humans; D008251:Macaca; D008565:Membrane Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid; D015302:Simian Immunodeficiency Virus / Q000378:metabolism; D054311:nef Gene Products, Human Immunodeficiency Virus
D020756:Coatomer Protein; D017931:DNA Primers; D018076:DNA, Complementary; D015693:Gene Products, nef; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus