title: Complete cDNA sequence of human lysosome-associated membrane protein-2.
abstract: The isolation and sequencing of 15 independent human lysosome-associated membrane protein-2 (h-lamp-2) recombinants from a primary human liver cDNA library has resulted in the determination of a transcript sequence significantly longer than previously reported and reveals the utilization of each of the four potential polyadenylation signals (AATAAA) present in the 3' untranslated region. The most 5' extending cDNA clone initiates upstream of the proposed transcription initiation site. A number of differences with published sequences for the h-lamp-2 transcript were observed, some of which result in amino acid changes in the predicted primary structure of the h-lamp-2 protein, and two of which give rise to restriction fragment length polymorphisms. The knowledge of these sequence alterations and polymorphisms is an important consideration for the further analysis of the h-lamp-2 locus with regard to the delineation of function and association with human inherited disorders.
authors.affiliation: Molecular Genetic Research Group, University Childrens Hospital, Heidelberg, FRG.
authors.forename: D S
authors.initials: DS
authors.lastname: Konecki
authors.forename: K
authors.initials: K
authors.lastname: Foetisch
authors.forename: M
authors.initials: M
authors.lastname: Schlotter
authors.forename: U
authors.initials: U
authors.lastname: Lichter-Konecki
mesh_terms: D000595:Amino Acid Sequence; D015703:Antigens, CD; D001483:Base Sequence; D018076:DNA, Complementary; D006801:Humans; D008099:Liver / Q000378:metabolism; D051907:Lysosomal Membrane Proteins; D008247:Lysosomes; D008562:Membrane Glycoproteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D015703:Antigens, CD; D018076:DNA, Complementary; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins