Resorption patterns following intentional vital root transection in Macaca mulatta.
Previous studies have shown degeneration of nerve tissue as well as reorganization by periodontal tissue in dental pulp chambers following intentional vital root transection in Macaca mulatta. The supporting structures of the teeth, as well as the root itself, have not been fully studied following such surgery. The purpose of this study was to observe histopathologic changes in bone and root structure over a 1-year postoperative period. Three adult Macaca mulatta were evaluated for this study. Roots were transected within the apical one third and the areas were surgically closed. Following a postoperative soft diet for 7 days, the monkeys were allowed normal masticatory function. Maxillary and mandibular quadrants were removed following vascular perfusion of the animals at 1, 2, 3, 6, 24, and 52 weeks; quadrants were then demineralized, embedded, sectioned, and stained with Preece's trichrome. Resorption of cementum, dentin, and bone was independently evaluated on microslides for each tooth at consecutive intervals of approximately 70 microns. Resorption was rated as mild, moderate, or severe depending on the number of Howship's lacunae and the dimension of the resorptive area. Severe bone resorption was evident at 1 week, decreasing at 2 weeks, and then progressively decreasing up to 52 weeks. Most active inflammatory root resorption was noted on maxillary and mandibular premolars and molars on first-week specimens. Arrested resorptive areas were present in similar locations in second- and third-week specimens. New cementum was present along the cut surface on specimens from 2 to 52 weeks and within the pulp canal beginning at 2 weeks.(ABSTRACT TRUNCATED AT 250 WORDS)
Allen
R
R
Department of Orthodontics, School of Dentistry, University of Alabama, Birmingham.
Sadowsky
P L
PL
Cox
C F
CF
D000818:Animals; D001047:Apicoectomy / Q000009:adverse effects*; D001861:Bone Regeneration; D005260:Female; D008253:Macaca mulatta; D010513:Periodontal Ligament / Q000293:injuries*; D010519:Periodontium / Q000473:pathology / Q000503:physiopathology; D012391:Root Resorption / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors; D018677:Tooth Injuries / Q000150:complications; D014092:Tooth Root / Q000293:injuries* / Q000601:surgery; D014945:Wound Healing