c-erbB-2 gene product associates with catenins in human cancer cells.
Phosphorylation of beta-catenin, an intracytoplasmic cadherin-binding protein, causes disruption of the cadherin-mediated cell adhesion system in cancer cells. A 185-kDa phosphorylated protein, identified as the c-erbB-2 gene product, was co-immunoprecipitated with the E-cadherin-catenin complex. Association of the c-erbB-2 gene product with the cadherin-catenin complex was proven to be mediated through beta-catenin and plakoglobin using an in vitro protein-protein precipitation system. These results indicate that the c-erbB-2 gene product associates with catenins and may regulate the cell adhesion and invasive growth of cancer.
Ochiai
A
A
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.
Akimoto
S
S
Kanai
Y
Y
Shibata
T
T
Oyama
T
T
Hirohashi
S
S
D015820:Cadherins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D051180:Desmoplakins; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D010766:Phosphorylation; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators; D014407:Tumor Cells, Cultured; D051176:beta Catenin; D051185:gamma Catenin
D015820:Cadherins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D051180:Desmoplakins; D018719:Erb-b2 Receptor Tyrosine Kinases; D015534:Trans-Activators; D051176:beta Catenin; D051185:gamma Catenin; C495270:CTNNB1 protein, human