Peroxidation of human blood lipoproteins induced by exogenous hypochlorite or hypochlorite generated in the system of "myeloperoxidase + H2O2 + Cl-".
Oxidation of human plasma lipoprotein (LP) was studied in the presence of exogenous hypochlorite anion (OCl-) or OCl- generated in the "myeloperoxidase + H2O2 + Cl-" system. OCl- effectively initiates peroxidation of lipids extracted from LP and those within LP particles, as can be judged from accumulation of secondary (thiobarbituric acid [TBA] reactive) and final (Schiff bases) products of lipid peroxidation (LPO) in LP after incubation with myeloperoxidase or exogenous OCl-. Very low density and low density lipoproteins classified as atherogenic LP are more sensitive to OCl(-)-induced LPO than high density lipoproteins. These data allow us to propose that OCl- secreted by activated neutrophils and monocyte-macrophages can produce oxidative modification of LP in vivo. The latter is known as a risk factor in the development of atherosclerosis.
Panasenko
O M
OM
Research Institute of Physico-Chemical Medicine, Moscow, Russia.
Evgina
S A
SA
Aidyraliev
R K
RK
Sergienko
V I
VI
Vladimirov
Y A
YA
D002712:Chlorides / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006997:Hypochlorous Acid / Q000494:pharmacology*; D007700:Kinetics; D015227:Lipid Peroxidation; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry / Q000187:drug effects; D008315:Malondialdehyde / Q000032:analysis; D009195:Peroxidase / Q000378:metabolism*; D012545:Schiff Bases; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D002712:Chlorides; D008074:Lipoproteins; D012545:Schiff Bases; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D009195:Peroxidase