LTE1 of Saccharomyces cerevisiae is a 1435 codon open reading frame that has sequence similarities to guanine nucleotide releasing factors.
The DNA sequence of the LTE1 gene on the left arm of chromosome I of Saccharomyces cerevisiae has been determined. The LTE1 open reading frame comprises 4305 bp that can be translated into 1435 amino acid residues. The position of this open reading frame corresponds well to that of a 4.7 kb transcript that has been mapped to this position. The derived amino acid sequence has significant similarities to the amino acid sequence of the guanine nucleotide releasing factor isolated from a rat brain library. The carboxy-terminus of the LTE1 protein also shows similarities to other guanine nucleotide exchange factors of the S. cerevisiae CDC25 family.
Keng
T
T
Biology Department, McGill University, Montreal, Canada.
Clark
M W
MW
Storms
R K
RK
Fortin
N
N
Zhong
W
W
Ouellette
B F
BF
Barton
A B
AB
Kaback
D B
DB
Bussey
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal; D013696:Temperature; D020663:ras Guanine Nucleotide Exchange Factors; D020666:ras-GRF1
D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; C089357:LTE1 protein, S cerevisiae; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D020663:ras Guanine Nucleotide Exchange Factors; D020666:ras-GRF1; D010749:Phosphoprotein Phosphatases