title: Murine PGK-1 promoter drives widespread but not uniform expression in transgenic mice.
abstract: Pgk-1 is an X-linked gene encoding 3-phosphoglycerate kinase, an enzyme necessary in every cell for glycolysis. The regulatory sequences of the Pgk-1 gene were used to drive the E. coli lacZ reporter gene and 2 strains of transgenic animals created with this Pgk-lacZ transgene carried on autosomes. The levels of expression of Pgk-1 varied from one adult tissue to another and the transgene was similarly regulated. However, in situ staining of the beta-galactosidase encoded by the transgene indicated extensive cell-to-cell variability in its level of expression. A reproducible subset of cells stained darkly for the transgene product. Some of these beta-galactosidase positive cells were rapidly proliferating while others appeared to be metabolically very active, suggesting that the Pgk-1 promoter is regulated so as to be more active in cells requiring high levels of glycolysis. Although Pgk-1 is X-linked and subject to X chromosome inactivation, the transgenes were not inactivated in either female somatic or male germ cells. Thus, the Pgk-1 promoter drives transgene expression in all tissues but the levels of expression are not uniform in each cell.
authors.affiliation: Department of Medicine, University of Ottawa, Ontario, Canada.
authors.forename: M W
authors.initials: MW
authors.lastname: McBurney
authors.forename: W A
authors.initials: WA
authors.lastname: Staines
authors.forename: K
authors.initials: K
authors.lastname: Boekelheide
authors.forename: D
authors.initials: D
authors.lastname: Parry
authors.forename: K
authors.initials: K
authors.lastname: Jardine
authors.forename: L
authors.initials: L
authors.lastname: Pickavance
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D004622:Embryo, Mammalian / Q000502:physiology; D005314:Embryonic and Fetal Development; D005260:Female; D015870:Gene Expression; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012727:Sex Characteristics
chemical_list: D010735:Phosphoglycerate Kinase