Transient induction of fatty acid synthase in rat liver after removal of a peroxisome proliferator.
Removal of a peroxisome proliferator from the diet triggered the degradation of peroxisomes and induced the transient expression of a 220 kDa soluble protein in rat liver. The 220 kDa protein was purified by conventional methods and analyzed by amino acid sequencing. A total of 99 amino acid residues in 4 lysylendopeptidase-digested peptides completely matched those in rat fatty acid synthase. The transient induction of fatty acid synthase mRNA during peroxisome degradation was confirmed by Northern blotting.
Motojima
K
K
Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Isaka
M
M
Takino
Y
Y
Goto
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA; D004790:Enzyme Induction; D064429:Fatty Acid Synthases / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008830:Microbodies / Q000378:metabolism*; D008969:Molecular Sequence Data; D051381:Rats; D011916:Rats, Inbred F344
D004247:DNA; D064429:Fatty Acid Synthases