title: The large nonsymbiotic plasmid pRmeGR4a of Rhizobium meliloti GR4 encodes a protein involved in replication that has homology with the RepC protein of Agrobacterium plasmids.
abstract: The large plasmid, pRmeGR4a, of Rhizobium meliloti GR4 is a nonsymbiotic, self-transmissible replicon that shows a high degree of stability. Plasmid replication and stabilization functions are located on a 4.8-kb PstI fragment. Analysis of the nucleotide sequence of this DNA region revealed the presence of six open reading frames (ORFs) with coding capabilities; all are transcribed in the same direction. Only one of the ORFs (ORF3) seems to be essential for replication. Its predicted protein sequence shows homology with RepC, a protein that has been suggested to be involved in the replication of Agrobacterium Ri and Ti plasmids.
authors.affiliation: Departamento de Microbiología, Estación Experimental del zaidín, CSIC, Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Mercado-Blanco
authors.forename: J
authors.initials: J
authors.lastname: Olivares
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016962:Sinorhizobium meliloti / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C043650:RepC protein, Staphylococcus aureus