Genome fingerprinting of Salmonella typhi by pulsed-field gel electrophoresis for subtyping common phage types.
The genomic DNA of 39 strains of Salmonella typhi isolated from local residents and patients who had visited countries in the Asian region was analysed for restriction fragment length polymorphisms (RFLP). Pulsed-field gel electrophoretic (PFGE) analysis of Xba I- and Spe I-generated genomic restriction fragments established 22 PFGE types whereas phage typing differentiated the 39 isolates into 9 distinct phage types. This study showed that PFGE is more discriminatory than phage typing as it is capable of subtyping S. typhi strains of the same phage types. Genetic relatedness among the isolates was determined. Seven major clusters were identified at SABs of > 0.80 and the remaining 13 isolates were distributed into minor clusters which were related at SABs of less than 0.80. In conclusion, PFGE analysis in conjunction with distance matrix analysis served as a useful tool for delineating common S. typhi phage types of diverse origins from different geographical locales and separated in time.
Nair
S
S
Department of Microbiology, Faculty of Medicine, National University of Singapore, Kent Ridge.
Poh
C L
CL
Lim
Y S
YS
Tay
L
L
Goh
K T
KT
D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D006801:Humans; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D012485:Salmonella typhi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014435:Typhoid Fever / Q000453:epidemiology / Q000382:microbiology*
D004262:DNA Restriction Enzymes