Interleukin-2 (IL-2) production by human B-cell line.
A human B-cell line (Hairy-BM) constitutively secreting interleukin-2 (IL-2) was established from tumor tissue resected surgically from a patient with breast cancer. Hairy-BM was found to be 100% CD20+, 98% surface immunoglobulin (sIg) G+, 98% sIg kappa chain+, 100% HLA-DR+, 94% IL-2 receptor (IL-2R alpha), 98% IL-2R beta, and devoid of T-cell, monocyte, and natural killer cell surface antigens. The B-cell origin of Hairy-BM was also confirmed by clonally rearranged Ig heavy- and Ig light-chain genes. The growth of Hairy-BM expressing IL-2R was promoted by recombinant IL-2 (rIL-2) and anti-CD25 antibody significantly blocked the growth enhancement. IL-2 secretion from Hairy-BM was confirmed by radioimmunoassay. By using a sensitive polymerase chain reaction technique, we demonstrated that Hairy-BM expressed IL-2 mRNA, IL-2R alpha mRNA, and IL-2R beta mRNA. These findings indicate that certain B-cells not only produce, but also respond to IL-2 in an autocrine fashion with increased proliferation.
Katano
M
M
Department of Surgery, Saga Medical School, Japan.
Morisaki
T
T
Matsuo
T
T
Nakamura
M
M
Kubota
E
E
Hisatsugu
T
T
D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004279:DNA, Viral; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics; D004854:Herpesvirus 4, Human; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007376:Interleukin-2; D008565:Membrane Proteins; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins