Toward cataloguing all rice genes: large-scale sequencing of randomly chosen rice cDNAs from a callus cDNA library.
A large-scale sequence analysis of rice cDNA was performed for a library from rice callus cultured in a medium containing 1 p.p.m. of 2,4-dichlorophenoxyacetic acid. Random sequencing of 2778 cDNA clones generated 2259 non-redundant expressed sequence tags (ESTs). The strategy of sequencing cDNAs can yield quickly a large number of novel genes. After translation, 690 sequences showed a significant amino acid sequence similarity to sequences already known from PIR. The source of known proteins ranged from bacteria to human. In this report, the non-redundant set of 280 identified ESTs is analyzed in detail.
Sasaki
T
T
Rice Genome Research Program, National Institute of Agrobiological Resources/Institute of Society for Technoinnovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
Song
J
J
Koga-Ban
Y
Y
Matsui
E
E
Fang
F
F
Higo
H
H
Nagasaki
H
H
Hori
M
M
Miya
M
M
Murayama-Kayano
E
E
D000818:Animals; D018076:DNA, Complementary; D004798:Enzymes / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D006801:Humans; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D004798:Enzymes; D010940:Plant Proteins