title: The porcine mammary gland as a bioreactor for complex proteins.
abstract: The similar biological activity of rhPC and hPC indicates that porcine mammary gland can perform many of the processing reactions necessary for recombinant synthesis of complex human proteins and produce them at levels suitable for industrial bioreactor applications. The health of the transgenic pigs appeared unaffected by the expression of high levels of the heterologous protein. We suggest that one of the advantages of using the mammary gland as a bioreactor appears to be the high cell density relative to that of cell culture.
authors.affiliation: Department of Chemical Engineering, Virginia Polytechnic Institute and State University, Blacksburg 24061.
authors.forename: T
authors.initials: T
authors.lastname: Morcöl
authors.forename: R M
authors.initials: RM
authors.lastname: Akers
authors.forename: J L
authors.initials: JL
authors.lastname: Johnson
authors.forename: B L
authors.initials: BL
authors.lastname: Williams
authors.forename: F C
authors.initials: FC
authors.lastname: Gwazdauskas
authors.forename: J W
authors.initials: JW
authors.lastname: Knight
authors.forename: H
authors.initials: H
authors.lastname: Lubon
authors.forename: R K
authors.initials: RK
authors.lastname: Paleyanda
authors.forename: W N
authors.initials: WN
authors.lastname: Drohan
authors.forename: W H
authors.initials: WH
authors.lastname: Velander
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001709:Biotechnology; D005260:Female; D006031:Glycosylation; D006801:Humans; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000033:anatomy & histology / Q000378:metabolism*; D008892:Milk / Q000737:chemistry; D015394:Molecular Structure; D011486:Protein C / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D013552:Swine; D014812:Vitamin K / Q000378:metabolism
chemical_list: D011486:Protein C; D011994:Recombinant Proteins; D014812:Vitamin K