title: Neutrophil sequestration in lungs removed at surgery. The effect of microscopic emphysema.
abstract: Neutrophils within the lungs are considered to play an important role in the pathogenesis of pulmonary emphysema. We have studied the intravascular distribution of reinjected autologous 111In-labeled neutrophils in lung specimens resected 10 min after reinjection from 10 patients undergoing surgery for peripheral bronchogenic tumors. An excess of neutrophils relative to that expected for the 99mTc-labeled erythrocyte blood volume was confirmed in all specimens (range, 3- to 136-fold). In seven specimens which were completely examined, this excess displayed a skewed distribution, with a median neutrophil sequestration of 20-fold excess, and correlated with local blood volume (r = -0.51; p < 0.001). There was also a significant correlation between alveolar wall surface area per unit volume of lung (AWUV) and neutrophil excess, when randomly selected tissue blocks from each specimen were analyzed (r = 0.34, n = 51, p = 0.012). This same trend was demonstrated when whole specimen median values were considered (r = 0.64, n = 7, p = 0.07). Thus in areas of the lungs with lower AWUV values (increasing microscopic emphysema), fewer neutrophils were present. These studies add further support to the view that emphysema per se is not associated with an increased sequestration of pulmonary intravascular neutrophils.
authors.affiliation: Department of Medicine and Thoracic Surgery, City Hospital, Edinburgh, Scotland, U.K.
authors.forename: C
authors.initials: C
authors.lastname: Selby
authors.forename: E
authors.initials: E
authors.lastname: Drost
authors.forename: M
authors.initials: M
authors.lastname: Gillooly
authors.forename: E
authors.initials: E
authors.lastname: Cameron
authors.forename: D
authors.initials: D
authors.lastname: Lamb
authors.forename: W
authors.initials: W
authors.lastname: MacNee
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D001810:Blood Volume; D002283:Carcinoma, Bronchogenic / Q000097:blood* / Q000150:complications / Q000175:diagnosis* / Q000601:surgery; D005260:Female; D015902:Gamma Cameras; D006801:Humans; D007205:Indium Radioisotopes; D007958:Leukocyte Count; D008175:Lung Neoplasms / Q000097:blood* / Q000150:complications / Q000175:diagnosis* / Q000503:physiopathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000473:pathology*; D011013:Pneumonectomy; D011650:Pulmonary Alveoli / Q000473:pathology; D011652:Pulmonary Circulation; D011656:Pulmonary Emphysema / Q000097:blood* / Q000150:complications / Q000175:diagnosis* / Q000503:physiopathology / Q000601:surgery; D012044:Regression Analysis
chemical_list: D007205:Indium Radioisotopes