title: Experimental models for antiphospholipid studies.
abstract: Three types of Experimental Animal Models have been used to investigate APA. 1) Passive transfer of APA to nocive mice. Branch et al injected pregnant Balb/c mice with purified IgG APA from patients with recurrent fetal loss. 2) Active immunization with APA. Backimer et al immunized Balb/c mice with a human monoclonal APA carrying the pathogenic idiotypes. 3) Immunization with beta 2 glycoprotein I (beta 2 GPI) (APA cofactor). Using affinity chromatography, we separated two distinct populations of antibodies. One was specific for beta 2GPI without binding to PL. Another population had a dual specificity for PL and beta 2GPI.
authors.affiliation: LSU Medical Center, New Orleans 70112.
authors.forename: A E
authors.initials: AE
authors.lastname: Gharavi
authors.forename: A L
authors.initials: AL
authors.lastname: Aron
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000209:etiology / Q000276:immunology; D000818:Animals; D017152:Antibodies, Antiphospholipid / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D016736:Antiphospholipid Syndrome / Q000150:complications / Q000276:immunology; D001327:Autoimmune Diseases / Q000150:complications / Q000276:immunology; D002846:Chromatography, Affinity; D004195:Disease Models, Animal; D005260:Female; D005327:Fetal Resorption / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB; D010743:Phospholipids / Q000276:immunology; D011247:Pregnancy; D011817:Rabbits; D014611:Vaccination; D053482:beta 2-Glycoprotein I
chemical_list: D017152:Antibodies, Antiphospholipid; D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D010743:Phospholipids; D053482:beta 2-Glycoprotein I