ELISA for in vivo assessment of nonenzymatically glycated platelet glutathione peroxidase.
Using a combination of boronate affinity chromatography and ELISA methodology, a simple procedure was devised to selectively determine the in vivo glycated state of the platelet glutathione peroxidase (GSH-Px) from normal and diabetic subjects. The mean total GSH-Px levels in the normal (n = 14) and diabetic (n = 18) platelets were 1167 +/- 97 and 1007 +/- 73 ng/mg protein, respectively. The mean percentage glycated GSH-Px in the normal and diabetic platelets were 5.79 +/- 0.72% and 11.68 +/- 0.95%, respectively. When the percentage glycated GSH-Px was compared with the fructosamine values, a correlation coefficient of 0.71 was obtained. This indicates that the glycation status of platelet GSH-Px can be utilized as a sensitive, short-term index of plasma glucose levels.
Muruganandam
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A
Department of Chemistry and Biochemistry, University of Windsor, Ontario, Canada.
Tannous
M
M
Mutus
B
B
D000818:Animals; D000918:Antibody Specificity; D001792:Blood Platelets / Q000201:enzymology*; D015153:Blotting, Western; D001897:Boronic Acids / Q000737:chemistry; D002417:Cattle; D002846:Chromatography, Affinity; D003198:Computer Simulation; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000097:blood*; D006031:Glycosylation; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D016014:Linear Models
D001897:Boronic Acids; D007106:Immune Sera; C028592:3-aminobenzeneboronic acid; D005979:Glutathione Peroxidase