title: CD23 and IgE expression during the human immune response to cutaneous leishmaniasis: possible role in monocyte activation.
abstract: Leishmania brasiliensis causes cutaneous leishmaniasis (CL) in humans. During this infection, a variety of inflammatory mediators are produced by T cells and monocytes/macrophages. In the present study, we analysed serum IgE levels and their correlation with in situ expression of the low affinity receptor for IgE (Fc epsilon RII/CD23) in patients infected with L. brasiliensis before and following therapy. These analyses were compared to in situ expression of tumour necrosis factor-alpha (TNF alpha), interleukin 3 (IL3), interferon-gamma (IFN gamma) and IL4. Disease-free individuals from the same endemic area sensitized with L. brasiliensis antigens were also included in this work. Our data indicate that during infection, serum levels of IgE and TNF alpha increased and correlated with elevated in situ expression of CD23, IL4 and TNF alpha mRNA. This expression disappeared following successful treatment, but persisted in patients resistant to anti-leishmania therapy. Patients resistant to therapy differed from other cases by a dramatic decrease in their in vivo expression of IFN gamma protein. Analysis of CD23 function in purified human monocytes indicated that this antigen mediates IgE/anti-IgE-dependent TNF alpha production. These data suggest a possible in vivo role of CD23 in acute immune responses in human CL.
authors.affiliation: Laboratoire de Parasitologie Expérimentale, INSERM U313, Paris.
authors.forename: I
authors.initials: I
authors.lastname: Vouldoukis
authors.forename: F
authors.initials: F
authors.lastname: Issaly
authors.forename: C
authors.initials: C
authors.lastname: Fourcade
authors.forename: N
authors.initials: N
authors.lastname: Paul-Eugène
authors.forename: M
authors.initials: M
authors.lastname: Arock
authors.forename: J P
authors.initials: JP
authors.lastname: Kolb
authors.forename: O A
authors.initials: OA
authors.lastname: da Silva
authors.forename: L
authors.initials: L
authors.lastname: Monjour
authors.forename: H
authors.initials: H
authors.lastname: Poinsot
authors.forename: Y
authors.initials: Y
authors.lastname: Tselentis
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000981:Antiprotozoal Agents / Q000008:administration & dosage / Q000627:therapeutic use; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007892:Leishmania braziliensis / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D009000:Monocytes / Q000276:immunology*; D015800:Protozoan Proteins / Q000008:administration & dosage; D012333:RNA, Messenger / Q000378:metabolism; D017455:Receptors, IgE / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000981:Antiprotozoal Agents; D016207:Cytokines; D015800:Protozoan Proteins; D012333:RNA, Messenger; D017455:Receptors, IgE; D007073:Immunoglobulin E