Separation of enantiomers of N-acetylcysteine by capillary electrophoresis after derivatization by o-phthaldialdehyde.
The optical purity of N-acetyl-L-cysteine was tested by capillary electrophoresis. The D-enantiomer can be found down to a ratio of 0.4:99.6 within 4 min without the use of the D-form or the racemate as a standard. The efficiency was optimized during method development up to a theoretical plate number, N, of several hundred thousand. The migration order and the separation mechanism was explained. The assumed structure of the formed isoindole S-[2-(1-carboxy-2-methylpropyl)isoindole-1-yl]-N-acetylcysteine diastereomers was confirmed and its stability was examined.
Dette
C
C
Institute of Pharmacy and Food Chemistry, University of Würzburg, Germany.
Wätzig
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H
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D007210:Indoleacetic Acids / Q000737:chemistry*; D054833:Isoindoles; D015394:Molecular Structure; D013237:Stereoisomerism; D009764:o-Phthalaldehyde
D007210:Indoleacetic Acids; D054833:Isoindoles; C090731:S-(2-(1-carboxy-2-methylpropyl)isoindole-1-yl)-N-acetylcysteine; D009764:o-Phthalaldehyde; D000111:Acetylcysteine