title: Effect of simple carbohydrates, casein hydrolysate, and a lipid test meal on ethane exhalation rate.
abstract: To determine the potential differences in the effect of various nutrients on lipid peroxidation, the ethane exhalation (EE) rate, an index of lipid peroxidation, was measured in rats at 4 (young), 18 (intermediate age), and 24 (aged) mo of age at fasting conditions and after acute ingestion of various test meals. The EE rate (means +/- SD) after a 15-h fast was significantly reduced in 24-mo-old rats (2.45 +/- 0.44 pmol.min-1.100 g body wt-1) and 18-mo-old rats (3.51 +/- 0.55 pmol.min-1.100 g body wt-1) compared with 4-mo-old rats (4.44 +/- 0.66 pmol.min-1.100 g body wt-1; P < 0.01). The EE rate significantly increased in 4-mo-old rats after ingestion of 50% (wt/vol) dextrose (8.59 +/- 2.9 pmol.min-1.100 g body wt-1), 50% casein hydrolysate (6.77 +/- 1.23 pmol.min-1.100 g body wt-1), and 20% neutral lipid emulsion (7.33 +/- 1.96 pmol.min-1.100 g body wt-1; P < 0.01). The response of aged rats to these nutrients compared with young rats was reduced by approximately 50%. A 25% dextrose solution or a 50% solution of sucrose, fructose, maltose, or galactose did not significantly alter EE rate. It is concluded that various macronutrients have a diverse potential of inducing lipid peroxidation. The responsiveness of aged rats to meal-induced enhancement of EE and presumably lipid peroxidation is significantly reduced.
authors.affiliation: St. Louis Veteran Affairs Medical Center, Missouri 63104.
authors.forename: A D
authors.initials: AD
authors.lastname: Mooradian
authors.forename: M P
authors.initials: MP
authors.lastname: Habib
authors.forename: F
authors.initials: F
authors.lastname: Dickerson
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D002364:Caseins / Q000494:pharmacology*; D004032:Diet; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology*; D004980:Ethane / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D011492:Protein Hydrolysates / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D002364:Caseins; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D005947:Glucose; D004980:Ethane