Basal and thrombin-stimulated platelet cell calcium.
To study platelet cell calcium in dogs, we measured the fura-2-determined calcium concentration (Cai) in platelets derived from blood drawn through indwelling arterial catheters in four dogs. Platelet samples were obtained 3 days a week for 39 days. Canine platelet Cai averages 40.5 +/- 1.7 nmol/L when corrected for extracellular fura-2 dye (n = 46); this mean is significantly smaller than the value obtained without such a correction (50.2 +/- 2.0 nmol/L, P < .05). The amplitude of thrombin-induced Ca2+ transients is proportional to basal Cai, increasing 4.9 +/- 0.9 nmol/L peak Ca2+ per nmol/L increase in baseline Ca2+.
Dwyer
T M
TM
Department of Physiology, University of Mississippi Medical Center, Jackson.
Diveki
K
K
Newton
G
G
Szebeni
A
A
Hildebrandt
D
D
Petrini
M
M
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D004285:Dogs; D016257:Fura-2; D006973:Hypertension / Q000097:blood; D008345:Manganese / Q000097:blood; D013917:Thrombin / Q000494:pharmacology*
D008345:Manganese; D013917:Thrombin; D002118:Calcium; D016257:Fura-2