Isolation of mouse complement component C7.
Mouse complement component C7 was purified from serum by a sequential procedure of fractionation precipitation by ammonium sulfate, followed by DE-52 anion exchange chromatography. Protein G affinity column chromatography, Mono S cation exchange chromatography and Superdex 200 gel filtration. The final product contained a highly purified mouse C7 component showing a single band on SDS-PAGE at the apparent Mrs of 90 kDa and 100 kDa under non-reduced and reduced conditions respectively. The yield of C7, which was measured by the biological activity, was 7.0%
Hitsumoto
Y
Y
Department of Clinical Laboratory Medicine, Ehime University School of Medicine, Japan.
Ohnishi
H
H
Okada
M
M
Nishimukai
H
H
Saheki
S
S
Takeuchi
N
N
D000818:Animals; D005591:Chemical Fractionation; D002845:Chromatography / Q000379:methods; D003184:Complement C7 / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008811:Mice, Inbred DBA
D003184:Complement C7