Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to Dot-ELISA.
The alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) was assessed in dot-ELISA for the diagnosis of Chagas' disease. Serum samples (355) from chagasic and non-chagasic patients were studied, and IgG antibodies to ASEA were found in all patients with chronic Chagas' disease. In non-chagasic patients 95.6% were negative, except for those with leishmaniasis (visceral and mucocutaneous), and some patients from control group reacted in low titers. The data indicate that dot-ELISA using ASEA is suitable for seroepidemiologic surveys to be employed in endemic areas for Chagas' disease.
Lissaldo
A M
AM
Instituto de Ciências Biomédicas, USP, São Paulo, Brasil.
Hoshino-Shimizu
S
S
Umezawa
E S
ES
Stolf
A M
AM
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D014355:Chagas Disease / Q000097:blood / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan