title: A comparative histologic study of the pulp-dentinal interface in undemineralized and demineralized tooth sections.
abstract: This study examines cellular differences between undemineralized and demineralized tooth sections. The material comprised unerupted and partly erupted third molars. After fixation for 24 h, 10- to 15-microns ground sections were prepared from half of the tooth, using a cutting-grinding system. The sections were compared with demineralized routine sections from the other half of the tooth. Undemineralized sections showed a better capacity for preserving the tissue integration. Especially between the odontoblast layer and the predentin a low frequency of disjunctions or tears were noted. At this level of examination fixation quality and vacuole frequency (intra-cellular spaces) did not show any differences between the two methods.
authors.affiliation: Department of Cariology and Endodontics, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Bjørndal
authors.forename: A
authors.initials: A
authors.lastname: Thylstrup
mesh_terms: D002462:Cell Membrane / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure; D003648:Decalcification Technique; D003782:Dental Pulp / Q000166:cytology*; D003804:Dentin / Q000166:cytology*; D005110:Extracellular Space; D016591:Histocytological Preparation Techniques; D006801:Humans; D008964:Molar, Third / Q000166:cytology; D009804:Odontoblasts / Q000166:cytology; D016707:Tissue Fixation; D014078:Tooth Eruption; D014097:Tooth, Unerupted / Q000473:pathology; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D002843:Chromatin