Accumulation of HLA-DM, a regulator of antigen presentation, in MHC class II compartments.
The HLA-DM genes encode an unconventional HLA (human leukocyte antigen) class II molecule that is required for appropriate binding of peptide to classical HLA class II products. In the absence of DM, other class II molecules are unstable upon electrophoresis in sodium dodecyl sulfate and are largely associated with a nested set of peptides derived from the invariant chain called CLIP, for class II-associated invariant chain peptides. DMA and DMB associated and accumulated in multilaminar, intracellular compartments with classical class II molecules, but were found infrequently, if at all, at the cell surface. Thus, DM may facilitate peptide binding to class II molecules within these intracellular compartments.
Sanderson
F
F
Human Immunogenetics Laboratory, Imperial Cancer Research Fund, Holborn, London, UK.
Kleijmeer
M J
MJ
Kelly
A
A
Verwoerd
D
D
Tulp
A
A
Neefjes
J J
JJ
Geuze
H J
HJ
Trowsdale
J
J
D000818:Animals; D017951:Antigen Presentation; D002451:Cell Compartmentation; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D004721:Endoplasmic Reticulum / Q000276:immunology; D005802:Genes, MHC Class II; D006681:HLA-D Antigens / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007739:L Cells; D051379:Mice; D016253:Microscopy, Immunoelectron; D013347:Subcellular Fractions / Q000276:immunology; D014407:Tumor Cells, Cultured
C416189:H2-M antigens; D006681:HLA-D Antigens; C084245:HLA-DM antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II