The response of quiescent cell populations in murine solid tumors to irradiation with fast neutrons.
5-bromo-2'-deoxyuridine (BUdR) was injected into SCC VII tumor-bearing mice intraperitoneally to label all proliferating tumor cells. The mice were irradiated with fast neutrons or x-rays. Immediately, or 24 h after irradiation, the tumors were excised, minced and trypsinized. The tumor cell suspensions were incubated with cytochalasin-B (a cytokinesis blocker). The micronucleus frequency was determined using immunofluorescence staining to BUdR. The cells that were not labeled with BUdR could be regarded as the quiescent cells. The micronucleus frequency in total tumor cells was determined from the irradiated tumors that were not pretreated with BUdR. The difference in radiosensitivity between total and quiescent cells was markedly reduced with fast neutrons, especially at higher doses of radiation. Potentially lethal damage repair by total and quiescent cells was inhibited more strongly with neutrons than with x-rays. When using fast neutrons, the radiosensitivity of solid tumors depends on their heterogeneity less critically than for x-rays.
Masunaga
S
S
Radiation Oncology Research Laboratory, Kyoto University, Osaka, Japan.
Ono
K
K
Akuta
K
K
Akaboshi
M
M
Abe
M
M
Ando
K
K
Koike
S
S
D000818:Animals; D001973:Bromodeoxyuridine; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000532:radiotherapy*; D002467:Cell Nucleus / Q000648:ultrastructure; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000032:analysis*; D004307:Dose-Response Relationship, Radiation; D005214:Fast Neutrons; D005260:Female; D005455:Fluorescent Antibody Technique; D018499:Linear Energy Transfer; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D011836:Radiation Tolerance; D011882:Radiotherapy, High-Energy
D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine