Fast protein sequence verification by matrix assisted laser desorption mass spectrometric analysis of whole enzymatic digests.
Matrix assisted laser desorption mass spectrometry provides a very fast and efficient way to check protein sequences by the analysis of whole proteolytic digests. This method has been applied to the sequence verification of E. coli isoleucyl tRNA synthetase, for which two different sequences are found in data banks. The result of this investigation clearly shows that no one of these sequences is correct.
Hoang
B M
BM
Laboratoire de Biochimie, CNRS URA 240, Ecole Polytechnique, Palaiseau, France.
Dessen
P
P
Schmitter
J M
JM
D000595:Amino Acid Sequence; D010450:Endopeptidases; D007533:Isoleucine-tRNA Ligase / Q000737:chemistry / Q000378:metabolism; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D008666:Metalloendopeptidases; D008718:Methionine-tRNA Ligase / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis / Q000379:methods*; D012697:Serine Endopeptidases; D014357:Trypsin
D010450:Endopeptidases; D012697:Serine Endopeptidases; D014357:Trypsin; D008666:Metalloendopeptidases; C058921:endoproteinase Asp-N; D008718:Methionine-tRNA Ligase; D007533:Isoleucine-tRNA Ligase