The original pink-eyed dilution mutation (p) arose in Asiatic mice: implications for the H4 minor histocompatibility antigen, Myod1 regulation and the origin of inbred strains.
Allelic variation of the mouse pink-eyed dilution (p) gene in common laboratory strains and wild mice was examined by Southern blot and by polymerase chain reaction. In these assays the original p mutation allele found in strains SJL/J, 129/J, B10.129(21m), P/J and FS/Ei most closely matches an Asian Mus musculus allele, confirming anecdotal accounts of the Asian origin of this mutation. In contrast, the wild-type allele found in other common laboratory strains was apparently derived from Mus domesticus. Analysis of chromosome 7 loci both proximal and distal to the p locus demonstrates that strains SJL/J, 129/J, B10.129(21M), P/J and FS/Ei contain DNA segments of varying length derived from M. musculus. Strains 129/J and B10.129(21M) contain the largest segment of M. musculus-derived DNA (about 5 cM), including the loci Myod1, p, three clustered GABAA receptor subunit loci (Gabrg3, Gabra5 and Gabrb3), and Snrpn. The difference in the species origin of genes from this region of chromosome 7 may underlie the basis of the antigenicity of the minor histocompatibility antigen H4, defined by the strain B10.129(21M), and may account for the enhanced Myod1 activity observed in SJL/J mice.
Brilliant
M H
MH
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Ching
A
A
Nakatsu
Y
Y
Eicher
E M
EM
D000483:Alleles; D000818:Animals; D000835:Animals, Wild / Q000235:genetics / Q000276:immunology; D001208:Asia; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005127:Eye Color / Q000235:genetics*; D005786:Gene Expression Regulation; D051379:Mice / Q000235:genetics* / Q000276:immunology; D008815:Mice, Inbred Strains / Q000235:genetics / Q000276:immunology; D015778:Minor Histocompatibility Antigens / Q000235:genetics; D008912:Minor Histocompatibility Loci; D008969:Molecular Sequence Data; D009154:Mutation; D017570:MyoD Protein / Q000235:genetics; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015778:Minor Histocompatibility Antigens; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D004247:DNA