[Analysis of T cell receptor gene of infiltrating T lymphocytes in the pancreas of insulin-dependent diabetic patients].
Insulin-dependent diabetes mellitus (IDDM) develops mainly from the destruction of pancreatic beta cells by cytotoxic T lymphocytes. One of the key phenomena observed in the pancreas of IDDM patients is the destructive process of beta cells by cytotoxic T cells. In NOD mice, an animal model of IDDM, the T cell receptor (TCR) of the infiltrating T lymphocytes was reported to be variable. Our analysis of RNAs obtained from the pancreas of newly diagnosed IDDM patients using the RT-PCR method indicated that the repertoire of T cell receptor alpha was restricted. Clustering of conservative amino acid sequences was found in each patient. The elucidation of the TCR of T cells recognizing and destructing beta cells in IDDM would enable us to develop a novel method immunologically intervening the occurrence of IDDM.
Hanafusa
T
T
Second Department of Internal Medicine, Osaka University Medical School.
Yamagata
K
K
Nakajima
H
H
D000818:Animals; D015551:Autoimmunity; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D011948:Receptors, Antigen, T-Cell