8-Hydroxy-2'-deoxyguanosine formation and DNA damage induced by sulfur trioxide anion radicals.
The 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation and DNA damage by sulfur trioxide anion radicals (SO3.-) were investigated using ESR spin trapping, HPLC, and electrophoretic assays. Sulfite (SO3(2-) autoxidation generated both hydroxyl (.OH) and SO3.- radicals. Oxidation of SO3(2-) by chromium (VI) generated only SO3.- with much enhanced yield. Incubation of 2'-deoxyguanosine (dG) with SO3(2-) generated 8-OHdG albeit at low yield. Chromium (VI) enhanced the yield four-fold. Electrophoretic assays showed that SO3.- radicals generated by chromium (VI) oxidation of SO3(2-) caused DNA double strand breaks. The results demonstrate that SO3.- radicals are capable of causing dG hydroxylation and DNA double strand breaks.
Shi
X
X
Laboratory of Experimental Pathology, National Cancer Institute, Bethesda, MD 20892.
Mao
Y
Y
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000838:Anions; D002857:Chromium / Q000633:toxicity; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D004357:Drug Synergism; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D005609:Free Radicals; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D010084:Oxidation-Reduction; D013461:Sulfur Oxides / Q000737:chemistry / Q000633:toxicity*
D000838:Anions; D016166:Free Radical Scavengers; D005609:Free Radicals; D009153:Mutagens; D013461:Sulfur Oxides; D002857:Chromium; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; C011118:sulfur trioxide