title: Inhibitory effect of tetrahydroberberine on platelet aggregation and thrombosis.
abstract: Tetrahydroberberine (THB), an alkaloid extracted from Corydalis ambigua, inhibited the rabbit platelet aggregation triggered by arachidonic acid (AA), ADP, and collagen with IC50 of 0.86, 1.31, and 1.10 mmol.L-1, respectively. THB reduced the thromboxane B2 (TXB2) generation in rabbit platelet-rich plasma triggered by AA. THB 30 mg.kg-1.d-1 ip for 3 or 5 d restrained the ADP-induced platelet aggregation in rats. THB 30 mg.kg-1.d-1 ip for 1, 3, or 5 d inhibited the AA-induced platelet aggregation in rats. THB 15-30 mg.kg-1 iv showed an inhibition of venous thrombosis in rats. The results show that THB is a potent inhibitor of platelet aggregation in vitro and in vivo and is a promising antithrombotic drug.
authors.affiliation: Department of Pharmacology, Nanjing Medical College, China.
authors.forename: B
authors.initials: B
authors.lastname: Xuan
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: D X
authors.initials: DX
authors.lastname: Li
mesh_terms: D000244:Adenosine Diphosphate / Q000037:antagonists & inhibitors; D000818:Animals; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors; D001599:Berberine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013927:Thrombosis / Q000517:prevention & control*; D013929:Thromboxane B2 / Q000097:blood
chemical_list: D001095:Arachidonic Acids; D010975:Platelet Aggregation Inhibitors; D001599:Berberine; D013929:Thromboxane B2; D000244:Adenosine Diphosphate; C004645:canadine