title: Control of kinetic properties of AMPA receptor channels by nuclear RNA editing.
abstract: AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptor channels mediate the fast component of excitatory postsynaptic currents in the central nervous system. Site-selective nuclear RNA editing controls the calcium permeability of these channels, and RNA editing at a second site is shown here to affect the kinetic aspects of these channels in rat brain. In three of the four AMPA receptor subunits (GluR-B, -C, and -D), intronic elements determine a codon switch (AGA, arginine, to GGA, glycine) in the primary transcripts in a position termed the R/G site, which immediately precedes the alternatively spliced modules "flip" and "flop." The extent of editing at this site progresses with brain development in a manner specific for subunit and splice form, and edited channels possess faster recovery rates from desensitization.
authors.affiliation: Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Lomeli
authors.forename: J
authors.initials: J
authors.lastname: Mosbacher
authors.forename: T
authors.initials: T
authors.lastname: Melcher
authors.forename: T
authors.initials: T
authors.lastname: Höger
authors.forename: J R
authors.initials: JR
authors.lastname: Geiger
authors.forename: T
authors.initials: T
authors.lastname: Kuner
authors.forename: H
authors.initials: H
authors.lastname: Monyer
authors.forename: M
authors.initials: M
authors.lastname: Higuchi
authors.forename: A
authors.initials: A
authors.lastname: Bach
authors.forename: P H
authors.initials: PH
authors.lastname: Seeburg
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D005091:Exons; D018698:Glutamic Acid / Q000494:pharmacology; D005998:Glycine / Q000235:genetics; D007438:Introns; D007700:Kinetics; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D009865:Oocytes; D016716:PC12 Cells; D018408:Patch-Clamp Techniques; D017393:RNA Editing; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014981:Xenopus
chemical_list: D018091:Receptors, AMPA; D011994:Recombinant Proteins; D018698:Glutamic Acid; D005998:Glycine