Bovine x murine T-cell hybridomas specific for bovine herpesvirus 1 (BHV-1) glycoproteins.
Difficulties in the isolation and long-term maintenance of bovine herpesvirus-1 (BHV-1) specific T-cell clones have hindered the analysis of bovine cell-mediated immune response to this virus. In an effort to identify the T-cell epitopes of the virus, bovine murine T-cell hybridomas specific for BHV-1 were generated as an alternative to T-cell clones. Peripheral blood lymphocytes from a calf immunized with BHV-1 were restimulated in vitro with the virus to generate bulk T-cell cultures. The antigen-specific T-cell-enriched bulk culture lymphocytes were fused with the T-cell receptor-deficient mutant of the murine thymoma cell line BW 5147. T-cell hybridomas were screened for their ability to produce interferon-gamma in response to BHV-1 stimulation. Hybridomas with various specificities were obtained. One of them was specific for the BHV-1 glycoprotein gI, two were specific for gIV, while three other hybridomas were specific for gIII. One hybridoma responded to stimulation with BHV-1, but not to any of the glycoproteins gI, gIII, or gIV, suggesting that proteins other than these major glycoproteins may be involved in the bovine T-cell response to BHV-1. Of these hybridomas, one was MHC Class I restricted, while all the others were Class II restricted.
Nataraj
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C
Department of Veterinary and Biomedical Sciences, IANR, University of Nebraska-Lincoln.
Srikumaran
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S
D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology; D006825:Hybridomas; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology* / Q000302:isolation & purification
D006649:Histocompatibility Antigens; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins; D007371:Interferon-gamma