title: Characterization of Acinetobacter type strains and isolates obtained from wastewater treatment plants by PCR fingerprinting.
abstract: Acinetobacter type strains and isolates from wastewater treatment plants were differentiated by PCR fingerprinting. On the first level, PCR fingerprinting with two tRNA-gene specific primers (T5B and T3A) was used for the identification of species (genospecies 1 to 17). On the second level, a single arbitrary primer (DAF 4) was employed for strain differentiation. Upon comparison of Acinetobacter type strains with 28 sewage sludge isolates, 2 could be classified as belonging to A. johnsonii, 8 isolates could be classified as A. lwoffii, 8 could be classified as A. baumannii, and 9 isolates were very closely related to the Acinetobacter species A. junii; only 1 isolate could not be classified as one of the Acinetobacter type strains. The PCR fingerprinting method was found to be a reproducible and fast method for differentiation and identification of Acinetobacter isolates. Because of some resulting discrepancies compared with previously described identification schemes, e.g., DNA-DNA hybridization methods, the original identification experiments should be repeated and the results should be reassessed.
authors.affiliation: Institut für Biologie II/Mikrobiologie, Freiburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Wiedmann-al-Ahmad
authors.forename: H V
authors.initials: HV
authors.lastname: Tichy
authors.forename: G
authors.initials: G
authors.lastname: Schön
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012722:Sewage; D013045:Species Specificity; D018505:Waste Management; D014871:Water Microbiology
chemical_list: D012722:Sewage