Immunization with anticardiolipin cofactor (beta-2-glycoprotein I) induces experimental antiphospholipid syndrome in naive mice.
Beta-2-GPI is a 50 kDa glycoprotein which is known to be a serum co-factor, with a role in determining the binding of pathogenic anticardiolipin antibodies to phospholipids. Immunization of naive mice with beta-2-GPI resulted in elevated levels of antibodies directed against negatively charged phospholipids (cardiolipin, phosphotidylserine, phosphatidylinositol). The presence of increased titres of antiphospholipid antibodies in the sera of the mice was later followed by prolonged activated partial thromboplastin time (APTT), thrombocytopenia, and when the mice were mated, by a high percentage of fetal resorptions in the uterus. These data point to the ability of beta-2-GPI to induce pathogenic anti-cardiolipin antibodies following active immunization.
Blank
M
M
Department of Medicine B, Sheba Medical Center, Tel-Hashomer, Israel.
Faden
D
D
Tincani
A
A
Kopolovic
J
J
Goldberg
I
I
Gilburd
B
B
Allegri
F
F
Balestrieri
G
G
Valesini
G
G
Shoenfeld
Y
Y
D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis; D017152:Antibodies, Antiphospholipid / Q000096:biosynthesis*; D000918:Antibody Specificity; D016736:Antiphospholipid Syndrome / Q000209:etiology*; D001324:Autoantigens / Q000276:immunology; D001327:Autoimmune Diseases / Q000209:etiology*; D001772:Blood Cell Count; D004195:Disease Models, Animal; D005260:Female; D005327:Fetal Resorption / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D016682:Lupus Coagulation Inhibitor / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D010314:Partial Thromboplastin Time; D013921:Thrombocytopenia / Q000276:immunology; D014611:Vaccination; D053482:beta 2-Glycoprotein I
D000974:Antibodies, Antinuclear; D017152:Antibodies, Antiphospholipid; D001324:Autoantigens; D006023:Glycoproteins; D016682:Lupus Coagulation Inhibitor; D053482:beta 2-Glycoprotein I