title: Ultrastructural localization of calcium in neuromuscular junctions of smooth and skeletal muscles after aminoglycoside antibiotics treatment.
abstract: Aminoglycoside antibiotics are all capable of producing clinically significant neuromuscular paralysis. Since part of the mechanism of action of these antibiotics at neuromuscular junction is a calcium-dependent inhibition of acetylcholine release, so this experiment was carried out in vitro on both somatic (isolated rat phrenic-nerve hemidiaphragm) and autonomic neuro-effector transmission (guinea-pig ileum) using gentamicin and amikacin, to determine the calcium contents at this level. Electron microscopic observations on gentamycin- and/or amikacin-treated materials, using potassium pyroantimonate method suggest a reduction of internal calcium in nerve terminals of both preparations.
authors.affiliation: Department of Biology, Darou Pakhsh Co., Tehran, Iran.
authors.forename: P
authors.initials: P
authors.lastname: Nouhnejad
authors.forename: A R
authors.initials: AR
authors.lastname: Dehpour
authors.forename: T
authors.initials: T
authors.lastname: Samadian
authors.forename: S
authors.initials: S
authors.lastname: Amini
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002118:Calcium / Q000302:isolation & purification*; D003964:Diaphragm / Q000294:innervation; D005260:Female; D005839:Gentamicins / Q000494:pharmacology; D006168:Guinea Pigs; D007082:Ileum / Q000294:innervation; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000187:drug effects / Q000294:innervation; D009130:Muscle, Smooth / Q000737:chemistry* / Q000187:drug effects / Q000294:innervation; D009469:Neuromuscular Junction / Q000737:chemistry* / Q000187:drug effects; D010791:Phrenic Nerve; D051381:Rats; D013569:Synapses / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D000583:Amikacin; D002118:Calcium