title: Serine residue 45 of nodulation protein NodF from Rhizobium leguminosarum bv. viciae is essential for its biological function.
abstract: A system for testing the role of the Rhizobium nodF gene in the production of host-specific lipochitin oligosaccharides and in nodulation was developed. We show that a mutant nodF gene, in which the codon for serine residue 45 was changed to that for threonine, still expresses NodF, which, however, is no longer functional.
authors.affiliation: Clusius Laboratory, Institute of Molecular Plant Sciences, Leiden University, The Netherlands.
authors.forename: T
authors.initials: T
authors.lastname: Ritsema
authors.forename: O
authors.initials: O
authors.lastname: Geiger
authors.forename: P
authors.initials: P
authors.lastname: van Dillewijn
authors.forename: B J
authors.initials: BJ
authors.lastname: Lugtenberg
authors.forename: H P
authors.initials: HP
authors.lastname: Spaink
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D007887:Fabaceae / Q000382:microbiology; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000382:microbiology*; D010946:Plants, Medicinal; D016961:Rhizobium leguminosarum / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000235:genetics; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides; C068605:nodF protein, Rhizobium; D012694:Serine