Apolipoprotein E inhibition of proliferation of mitogen-activated T lymphocytes: production of interleukin 2 with reduced biological activity.
Apolipoprotein E (apoE), but not apoAI or apoCIII, suppresses mitogen-activated T lymphocyte proliferation, independent of the type of activation signal. Both CD4 and CD8 T cells are inhibited. ApoE inhibits T cell proliferation, in part, by reducing the production of bioactive interleukin 2 (IL2). IL2 activity is reduced by approximately 50-65% in cultures of mitogen-stimulated T cells when apoE is present. ApoE does not significantly alter levels of IL2 mRNA or the mass of secreted IL2 protein, quantitated by enzyme immunoassay. Reduced IL2 activity was not a consequence of induction of IL2 inhibitors in response to apoE or effects of apoE on the bioassay. These results suggest that apoE antagonizes post-translational events in mitogen-activated T lymphocytes that are required for the secretion of a bioactive IL2 protein.
Kelly
M E
ME
Developmental Biology Program, University of Cincinnati College of Medicine, Ohio 45267-0575.
Clay
M A
MA
Mistry
M J
MJ
Hsieh-Li
H M
HM
Harmony
J A
JA
D001057:Apolipoproteins E / Q000302:isolation & purification / Q000502:physiology*; D002478:Cells, Cultured; D004261:DNA Replication / Q000502:physiology; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D001057:Apolipoproteins E; D007376:Interleukin-2; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate