title: [The effect of deformation on the catalytic activity of carboxypeptidase A in the crystalline state].
abstract: A new approach to study of enzyme mechano-chemistry is proposed which is based on measuring of catalytic activity of crystalline cross-linked samples subject to uniaxial tension. Deformation of monoclinic P2(1) carboxypeptidase A crystals along [010] directions results in considerable increase of their esterase activity with no changes in the Michaelis constant. This can be explained as a consequence of considerable conformational changes accompanying the enzyme cycle.
authors.forename: T A
authors.initials: TA
authors.lastname: Zenchenko
authors.forename: V N
authors.initials: VN
authors.lastname: Morozov
mesh_terms: D002268:Carboxypeptidases / Q000737:chemistry / Q000378:metabolism*; D043422:Carboxypeptidases A; D002384:Catalysis; D003460:Crystallization; D011487:Protein Conformation
chemical_list: D002268:Carboxypeptidases; D043422:Carboxypeptidases A
vernacular_title: Vliianie deformatsii na kataliticheskuiu aktivnost' karboksipeptidazy A v kristallicheskom sostoianii.