title: Relationship between infectivity and OspC expression in Lyme disease Borrelia.
abstract: Borrelia burgdorferi sensu stricto strain 297 and B. garinii strains HP1 and 12-92 were serially subcultured for 36-50 passages in vitro for 1 year. All low-passage strains showed abundant expression of outer surface protein C (OspC) in the 22-23-kDa range, but the high-passage strains lost or showed reduced expression of OspC in comparison with the low-passage strains. The low-passage strains efficiently infected outbred ddY mice when inoculated into the hind footpad or peritoneal cavity. In contrast, the incidence of infection with the high-passage strains was low. Isolates from the bladders of mice inoculated with the high-passage strains expressed large amounts of OspC in comparison with those originally inoculated. These results indicate that OspC expression is related to the infectivity of Lyme disease borreliae.
authors.affiliation: Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Masuzawa
authors.forename: T
authors.initials: T
authors.lastname: Kurita
authors.forename: H
authors.initials: H
authors.lastname: Kawabata
authors.forename: Y
authors.initials: Y
authors.lastname: Yanagihara
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D015748:Borrelia burgdorferi Group / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D008193:Lyme Disease / Q000382:microbiology*; D051379:Mice
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; C079616:OspC protein