[Rapid in situ detection of chromosome 21 by the PRINS technique].
The PRINS technique has been used to rapidly detect chromosomes 21 in both metaphases and interphase nuclei. A specific labeling was obtained in preparations from lymphocytes and amniotic fluid cells. The method is based on annealing of specific oligonucleotide primers and subsequent primer extension by a Taq DNA polymerase. PRINS is an interesting alternative to in situ hybridization for cytogenetic diagnosis and physical mapping.
Pellestor
F
F
CNRS UPR 9008, Montpellier, France.
Girardet
A
A
Lefort
G
G
Andréo
B
B
Charlieu
J P
JP
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology; D001483:Base Sequence; D002891:Chromosomes, Human, Pair 21; D017931:DNA Primers; D004276:DNA, Satellite / Q000032:analysis; D004259:DNA-Directed DNA Polymerase; D004314:Down Syndrome / Q000175:diagnosis / Q000235:genetics; D005315:Fetal Diseases / Q000175:diagnosis; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000648:ultrastructure; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D019914:Taq Polymerase; D013997:Time Factors
D017931:DNA Primers; D004276:DNA, Satellite; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase
Détection rapide des chromosomes 21 in situ par la technique PRINS.