Simple and rapid HPLC of quinine, hydroxychloroquine, chloroquine, and desethylchloroquine in serum, whole blood, and filter paper-adsorbed dry blood.
A simple high-performance liquid chromatographic assay for the measurement of quinine, hydroxychloroquine, chloroquine, and desethylchloroquine in small volumes (50-200 microL) of serum, whole blood, or filter paper-adsorbed dry blood was developed. The analytes are isolated by liquid-liquid extraction and chromatographed on a sulfophenylpropyl-modified silica column under isocratic conditions. The column effluent is monitored by fluorimetry (excitation wavelength, 215 nm; no emission filter). The limit of quantitation is 0.005-0.01 mg/L for chloroquine and hydroxychloroquine and 0.05 mg/L for quinine. No interference from other antimalarial drugs or other compounds commonly used has been observed. Quantitative results can be obtained within 1 hour of receipt of the sample.
Croes
K
K
National Poisons Unit, NHS Trust, London, U.K.
McCarthy
P T
PT
Flanagan
R J
RJ
D000327:Adsorption; D002738:Chloroquine / Q000031:analogs & derivatives / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006886:Hydroxychloroquine / Q000097:blood*; D011803:Quinine / Q000097:blood*; D012680:Sensitivity and Specificity; D013048:Specimen Handling
D006886:Hydroxychloroquine; D002738:Chloroquine; D011803:Quinine; C007278:desethylchloroquine