Nucleotide sequence comparisons of the fusion protein gene from virulent and attenuated strains of rinderpest virus.
We have cloned and sequenced the entire fusion (F) protein gene of the RBOK vaccine strain of rinderpest virus and the coding regions for the F genes of two mild field isolates of the virus from Africa. Analysis of the nucleotide and the predicted amino acid sequences showed that the vaccine virus was more than 99% identical in the protein coding region to the virulent Kabete O strain from which it was derived, whereas the field isolates differed by 10 to 12% from each other and from the vaccine strain. No changes were found in the F protein which could explain attenuation of the vaccine; however, each of the mild field isolates had amino acid changes in important functional areas which may be related to their attenuated phenotype.
Evans
S A
SA
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
Baron
M D
MD
Chamberlain
R W
RW
Goatley
L
L
Barrett
T
T
D000349:Africa; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D015723:Gene Library; D005814:Genes, Viral / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012302:Rinderpest virus / Q000235:genetics* / Q000472:pathogenicity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014613:Vaccines, Attenuated / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics*
C079748:F protein, Rinderpest virus; D006023:Glycoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014613:Vaccines, Attenuated; D014760:Viral Fusion Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines