Measles serodiagnosis: standardization and evaluation of a Dot-ELISA.
A Dot-ELISA using a measles virus (MV) antigen obtained by sodium deoxycholate treatment was standardized and evaluated for IgM and IgG antibody detection in measles patients and measles-vaccinated subjects. A total of 192 serum samples were studied, comprising 47 from patients with acute and convalescent measles, 55 from 9-month old children prior to measles vaccination and 41 from children of the same age after vaccination, and 49 from patients with unrelated diseases. The diagnostic performances of the IgG Dot-ELISA and IgG immunofluorescence test (IFT) were found to be close, varying from 0.97 to 1.00 in sensitivity and the specificities were maximum (1.00). Nevertheless, the sensitivity of the IgM Dot-ELISA (0.85) was higher than that (0.63) of the IgM IFT, although both assays had comparably high (1.00) specificities. The IgM Dot-ELISA in particular proved to be more sensitive in relation to other assays studied by revealing antibodies in 80.0% (12/15) of vaccinated children on the 15th day after immunization. In contrast, the IgM IFT, failed to detect antibodies in the same group of vaccinated children. The stability of the MV antigen was longer than that of the IFT antigen, and the reproducibility of the Dot-Elisa was satisfactory.
Vaz De Lima
L R
LR
Seção de Imunologia, Instituto Adolfo Lutz, São Paulo, Brazil.
Hoshino-Shimizu
S
S
de Souza
V A
VA
Pannuti
C S
CS
Andrade Júnior
H F
HF
Sumita
L M
LM
Ferreira
A W
AW
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D008297:Male; D008457:Measles / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy; D008458:Measles Vaccine / Q000627:therapeutic use; D008459:Measles virus / Q000276:immunology; D012680:Sensitivity and Specificity
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008458:Measles Vaccine