Significant prolongation of guinea pig heart contraction transplanted in rat after removal of anti-xeno-antibodies by whole body rinse-out (WBRO) with hemoglobin solution.
OBJECTIVE
In order to investigate effectiveness of removal of the anti-xeno- antibodies in xenotransplantation (xeno Tx), WBRO using pyridoxalated-human hemoglobin-polyethyleneglycol conjugate (PHP solution) was performed prior to transplantation (Tx) of a guinea pig heart in a rat.


METHODS
Experiment I. Removal of the immunoglobulins and the anti-guinea pig lymphocytotoxic antibody (ALA) by WBRO. Exchange transfusion with the PHP solution was done in the Tx-expected rats until a hematocrit lowered below 5% (n = 11). Experiment II. Xeno heart Txs. Guinea pig hearts were transplanted into rats without immunosuppressants 1) without (n = 8) or 2) with the WBRO (n = 8).


RESULTS
Experiment I. Levels lowered to 14% in IgG, 17% in IgA and 6% in IgM, respectively, to initial values after the WBRO. An ALA titer lowered from 4 X (+) to 1 X (-) after the WBRO. Experiment II. An average heart contraction period was 10.4 +/- 1.8 minutes 1) without the WBRO in contrast to 472.5 +/- 4.8 minutes with the WBRO (p < 0.01).


CONCLUSIONS
WBRO using PHP solution is effective in removal of the anti-xeno-antibodies and consequent prolongation of survival of the xenograft.
Agishi
T
T
Department of Surgery, Kidney Center, Tokyo Women's Medical College, Japan.
Liu
H
H
Suga
H
H
Hayasaka
Y
Y
Ota
K
K
D000818:Animals; D000910:Antibodies, Heterophile / Q000302:isolation & purification*; D001802:Blood Substitutes / Q000008:administration & dosage*; D005069:Evaluation Studies as Topic; D006084:Graft Rejection / Q000517:prevention & control; D006168:Guinea Pigs; D016027:Heart Transplantation / Q000276:immunology / Q000379:methods* / Q000502:physiology; D006454:Hemoglobins / Q000008:administration & dosage*; D008297:Male; D009200:Myocardial Contraction; D010477:Perfusion; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D051381:Rats; D011917:Rats, Inbred Lew; D012996:Solutions; D013997:Time Factors; D014183:Transplantation, Heterologous
D000910:Antibodies, Heterophile; D001802:Blood Substitutes; D006454:Hemoglobins; D012996:Solutions; C050907:pyridoxalated-hemoglobin-polyethylene glycol conjugate; D011092:Polyethylene Glycols