The identification of nuclear and mitochondrial genes by sequencing randomly chosen clones from a marsupial mammary gland cDNA library.
To increase the number of genes that can be mapped to the genome of the tammar wallaby (Macropus eugenii), we sequenced 100 randomly chosen clones from a mammary gland cDNA library. Provisional identifications were made of seven nuclear genes and one mitochondrial gene encoding two caseins, beta-galactosidase, acetyl-coenzyme A synthetase, lipoprotein lipase, inorganic pyrophosphatase, an ATP-dependent RNA helicase, and cytochrome c oxidase I. Highly conserved genes, such as that encoding acetyl-coenzyme A synthetase, were easily identified even from cross-kingdom matches. Genes which are highly divergent, however, such as those encoding the mature casein peptides, could not be aligned with homologues in the databases. Even in an organ where there is high mRNA species redundancy, the sequence characterization of expressed sequence tags provides a rapid means of gene identification for mapping purposes.
Collet
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C
CSIRO, Division of Wildlife and Ecology, Canberra, Australia.
Joseph
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R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002467:Cell Nucleus; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D015723:Gene Library; D005796:Genes; D005819:Genetic Markers; D006801:Humans; D007614:Macropodidae / Q000235:genetics*; D008969:Molecular Sequence Data; D011897:Random Allocation; D016415:Sequence Alignment; D017385:Sequence Homology
D018076:DNA, Complementary; D004272:DNA, Mitochondrial; D005819:Genetic Markers