[Interaction of catalytically active antibodies with oligoribonucleotides].
The interaction of antibodies from blood sera of patients with autoimmune pathology, systemic lupus erythematosus with oligoribonucleotides was studied. The RNA-hydrolyzing activity was shown to be an intrinsic property of autoantibodies. Enzymic activity of antibodies in hydrolysis of poly(U) was estimated at 20-40% of that of RNase A. In contrast to known eukaryotic RNases, the autoantibodies possess a specific RNA-hydrolyzing activity for oligo r(A). The RNA-nicking activity of antibodies in hydrolysis of oligoadenylates was more higher than with hydrolysis of oligo d(A). Optimal conditions of r(pA)13 hydrolysis were selected, including the optimal of pH = 8.7.
Buneva
V N
VN
Andrievskaia
O A
OA
Romannikova
I V
IV
Gololobov
G V
GV
Iadav
R P
RP
Iamkovoĭ
V I
VI
Nevinskiĭ
G A
GA
D017151:Antibodies, Catalytic / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009843:Oligoribonucleotides / Q000276:immunology* / Q000378:metabolism
D017151:Antibodies, Catalytic; D001323:Autoantibodies; D009843:Oligoribonucleotides
Vzaimodeĭstvie kataliticheski aktivnykh antitel s oligoribonukleotidami.