Structural mannoproteins released by beta-elimination from Candida albicans cell walls.
Mild alkaline solutions (beta-elimination), after removing the non-covalently bonded wall materials by hot SDS, released 13% and 26% of remaining wall proteins from mycelial and yeast cells of Candida albicans, respectively. When the beta-elimination was carried out after digestion of the walls with chitinase, four-fold more proteinaceous materials were released from mycelium and a similar amount in yeast walls. The solubilized materials were shown to be highly polydisperse, and endo-glycosidase H reduced their polydispersity and molecular masses, revealing different electrophoretic patterns in yeast and mycelial cell walls. The solubilized mycelial proteins carried N-glycosidic sugar chains and the epitopes recognized by two monoclonal antibodies were preserved, although showing a different behaviour in yeast walls. These results are consistent with the idea that significant amounts of intrinsic O-glycosylated mannoproteins are interconnected in the walls of C. albicans.
Mormeneo
S
S
Departament de Microbiología, Facultat de Farmàcia, Universitat de València, Burjassot, Spain.
Marcilla
A
A
Iranzo
M
M
Sentandreu
R
R
D000911:Antibodies, Monoclonal; D002176:Candida albicans / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry*; D002688:Chitinases / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000737:chemistry; D008562:Membrane Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D011492:Protein Hydrolysates / Q000737:chemistry
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D011492:Protein Hydrolysates; C025363:mannoproteins; C037833:zymolyase; D006867:Hydrolases; D002688:Chitinases