An endogenous inhibitor of prostaglandin synthesis in human decidua.
Human first trimester decidual tissue has been reported to contain an inhibitor of cyclo-oxygenase enzyme, but a careful reading of the literature indicates that the inhibitor may be human albumin. Our own studies also showed that high levels of albumin were present in samples from homogenised decidual tissues. We therefore digested the tissue to obtain isolated cells and washed them extensively to minimise contamination with blood. Homogenates of such cells did not bind arachidonic acid, and inhibited cyclo-oxygenase enzyme-1 (COX-1) activity. The cellular factor inhibited COX-1 at concentrations of 60-100 micrograms protein/ml, and was therefore more potent than human albumin (0.5-2.0 mg/ml). The inhibitory activity was lost after heat treatment (100 degrees C for 10 mins) and after digestion with trypsin or papain, but was resistant to mercaptoethanol and urea. Preliminary data indicated that the protein had a molecular weight > 30 kD.
Sun
M Y
MY
R.P.M.S. Institute of Obstetrics and Gynaecology, Hammersmith Hospital, London, England.
Sullivan
M H
MH
Elder
M G
MG
D016718:Arachidonic Acid / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D003600:Cytosol / Q000737:chemistry; D003656:Decidua / Q000737:chemistry*; D015232:Dinoprostone / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D011261:Pregnancy Trimester, First; D012709:Serum Albumin / Q000494:pharmacology
D016861:Cyclooxygenase Inhibitors; D011257:Pregnancy Proteins; D012709:Serum Albumin; D016718:Arachidonic Acid; D015232:Dinoprostone