Application of the matrix solid phase dispersion technique for the determination of ivermectin residues in fish muscle tissue.
A modified high-performance liquid chromatographic method has been developed for the determination of ivermectin (IVM) residues in fish muscle tissue. The extraction and clean-up procedure is based on the matrix solid phase dispersion technique. Control and IVM-fortified fish muscle samples (0.5 g) are blended with octadecyl (C18 end-capped) packing material. A column made from the C18-fish tissue blend is washed with hexane (8 ml) and the IVM is eluted with 8 ml of dichloromethane-ethyl acetate (3 + 1). Ivermectin is derivatized and analyzed by high-performance liquid chromatography (HPLC) with fluorescence detection. The recovery from fortified samples was greater than 80% in the concentration range 20-100 ng g-1 of tissue. The limit of determination was 10 ng g-1 of tissue. This method and another, using solvent extraction and clean-up of the extract on a solid phase extraction cartridge, gave comparable results for an incurred sample containing IVM residue. This method incorporates a rapid sample pre-treatment step which makes it an attractive and useful method for routine analysis of IVM in fish muscle tissue.
Iosifidou
E
E
Department of Food Hygiene and Technology, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece.
Shearan
P
P
O'Keeffe
M
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D005399:Fishes; D007202:Indicators and Reagents; D007559:Ivermectin / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D007202:Indicators and Reagents; D007559:Ivermectin