title: Frozen-thawed cumulus-granulosa cells support bovine embryo development during coculture.
abstract: OBJECTIVE
  To evaluate the ability of bovine cumulus-granulosa cells to survive cryopreservation and subsequently support bovine embryo development during coculture.
  
  
  METHODS
  In vitro-matured and -fertilized bovine embryos (two- to four-cell) were allotted randomly to one of three treatment groups: [1] control medium alone consisting of Medium 199 containing 10% fetal bovine serum and antibiotics, [2] cocultured on fresh bovine cumulus-granulosa cells in control medium, or [3] cocultured on frozen-thawed cumulus-granulosa cells in control medium. Embryo development was assessed on days 7 and 8 after IVF.
  
  
  RESULTS
  Coculture improved embryo development on days 7 and 8 compared with the control group. However, embryo development on days 7 and 8 did not differ among coculture groups.
  
  
  CONCLUSIONS
  Frozen-thawed cumulus-granulosa cells enhance embryo development similar to fresh cells during in vitro coculture.
authors.affiliation: Department of Dairy Science, Louisiana State University, Baton Rouge.
authors.forename: J R
authors.initials: JR
authors.lastname: Broussard
authors.forename: J K
authors.initials: JK
authors.lastname: Thibodeaux
authors.forename: M W
authors.initials: MW
authors.lastname: Myers
authors.forename: J D
authors.initials: JD
authors.lastname: Roussel
authors.forename: S G
authors.initials: SG
authors.lastname: Prough
authors.forename: J
authors.initials: J
authors.lastname: Blackwell
authors.forename: R A
authors.initials: RA
authors.lastname: Godke
mesh_terms: D000818:Animals; D002417:Cattle / Q000196:embryology*; D046508:Culture Techniques / Q000379:methods; D005314:Embryonic and Fetal Development; D005260:Female; D005615:Freezing; D006107:Granulosa Cells / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology*