[Antiviral effect of recombinant interferon-alpha on hepatitis A virus replication in human liver cells].
Two recombinant interferons-alpha (IFNs-alpha) were assayed for their antiviral effect on hepatitis A virus (HAV) replication in the human hepatoma cell line PLC/PRF/5. IFN alpha-2a and IFN alpha-2b resulted in concentration-dependent inhibition of HAV antigen expression and HAV infectivity at non toxic concentrations. Their selectivity indices, calculated as the ratio of the dose that reduced the number of viable cells to 50% (CD50) to the effective dose that inhibited 50% of viral antigen expression (ED50) were > 1000. Recombinant IFN-alpha emerged, from the present study, as a promising candidate for chemotherapy of hepatitis A.
Crance
J M
JM
Unité de Biologie Moléculaire, Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Deloince
R
R
Leveque
F
F
Jouan
A
A
Trépo
C
C
D004305:Dose-Response Relationship, Drug; D006506:Hepatitis A / Q000628:therapy; D006507:Hepatovirus / Q000502:physiology*; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000821:virology*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured / Q000382:microbiology; D014779:Virus Replication / Q000187:drug effects
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins
Effet antiviral de l'interféron-alpha recombinant sur la multiplication du virus de l'hépatite A dans des cellules hépatiques humaines.