A function of CD10 on bone marrow stroma.
Bone marrow (BM) stromal cells express CD10 (cALLA), a surface antigen now known to be a neutral endopeptidase (NEP-24.11). The function of CD10 in BM stroma is unknown, although purified NEP-24.11 is known to degrade different substrates including interleukin 1 beta (IL-1 beta). We have therefore employed a CD10-positive BM stromal cell line (L2AK) which proliferates in response to IL-1 beta to test the hypothesis that degradation of this cytokine is one of the functions of stromal CD10. We first showed that [3H]thymidine incorporation by L2AK cells is enhanced by IL-1 beta in a clear dose-dependent manner. Addition of the CD10 inhibitor, phosphoramidon, together with IL-1 beta resulted in a left shift in the dose-response curve which corresponded to a 10-fold potentiation of the IL-1 beta effect. These results indicate that CD10 on bone marrow stromal cells can degrade IL-1 beta and therefore provide a local control of the effects of this, and possibly other, growth factor(s).
Delikat
S E
SE
Department of Haematolology, royal Liverpool University Hospital.
Galvani
D W
DW
Zuzel
M
M
D001853:Bone Marrow / Q000276:immunology* / Q000378:metabolism; D001854:Bone Marrow Cells; D002455:Cell Division; D002460:Cell Line; D006020:Glycopeptides / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D015260:Neprilysin / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000494:pharmacology; D017154:Stromal Cells / Q000276:immunology
D006020:Glycopeptides; D007375:Interleukin-1; D015260:Neprilysin; C008890:phosphoramidon