title: [Effect of panaxadiol saponin (PDS) on phorbol ester induced change of protein kinase C activity in cardiomyocytes].
abstract: Partially purified protein kinase C and protein kinase A were separately incubated with various concentrations of PDS (1-1000 micrograms.ml-1) for 10 min in vitro. The results indicate that protein kinase C activity was inhibited in a dose-dependent manner by PDS, but type I and type II protein kinase A were not. In cardiomyocytes preincubated with PDS (250, 500, 1000 micrograms.ml-1) for 10 min, PMA-induced decrease of cytosol protein kinase C activity and increase of membrane protein kinase C activity were greatly inhibited in the same manner. These results suggest that PDS not only inhibited protein kinase C in vitro, but also inhibited activation of protein kinase C in cardiomyocytes.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy Medical Sciences, Peking Union Medical College, Beijing.
authors.forename: X P
authors.initials: XP
authors.lastname: Xu
authors.forename: D M
authors.initials: DM
authors.lastname: Xiao
authors.forename: W H
authors.initials: WH
authors.lastname: Zhou
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.forename: J B
authors.initials: JB
authors.lastname: Zhang
authors.forename: C J
authors.initials: CJ
authors.lastname: Shao
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhao
authors.forename: H C
authors.initials: HC
authors.lastname: Chen
mesh_terms: D000818:Animals; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D036145:Ginsenosides; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D012503:Saponins / Q000494:pharmacology; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D036145:Ginsenosides; D010703:Phorbol Esters; D012503:Saponins; D014315:Triterpenes; C004564:panaxadiol; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C