Pharmacology of dopamine D3 receptors in the islands of Calleja of the rat using quantitative receptor autoradiography.
The pharmacological properties of [3H]7-hydroxy-N,N-di-n-propyl-2- aminotetralin ([3H]7-OH-DPAT) binding sites in the islands of Calleja of the rat were studied using quantitative receptor autoradiography. The KD and the Bmax values of [3H]7-OH-DPAT binding were about 1.6 nM and 100 fmol/mg protein, respectively. The rank order of potency was R(-)-propylnorapomorphine > 7-OH-DPAT approximately haloperidol > raclopride > dopamine > remoxipride. Remoxipride injected in vivo (0.03-100 mumol/kg i.p., 1 h before decapitation) did not inhibit subsequent [3H]7-OH-DPAT binding. These results indicate that the pharmacological profile of dopamine D3 receptors in the islands of Calleja of the rat resembles that obtained from cell lines or membrane preparations.
Hillefors-Berglund
M
M
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Von Euler
G
G
D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D018491:Dopamine Agonists / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009830:Olfactory Bulb / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011954:Receptors, Dopamine / Q000187:drug effects*; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D012044:Regression Analysis; D017330:Remoxipride / Q000494:pharmacology; D013764:Tetrahydronaphthalenes / Q000493:pharmacokinetics
D018491:Dopamine Agonists; D018492:Dopamine Antagonists; C493595:Drd3 protein, rat; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D013764:Tetrahydronaphthalenes; D017330:Remoxipride; C031349:7-hydroxy-2-N,N-dipropylaminotetralin