IL-4 induces chemotaxis of blood eosinophils from atopic dermatitis patients, but not from normal individuals.
T lymphocytes present in allergically inflamed tissue synthesize and secrete the cytokines interleukin (IL)-3, IL-4, IL-5, and granulocyte/macrophage colony-stimulating factor (GM-CSF). IL-3, IL-5, and GM-CSF, but also IL-4, may act as a chemotaxin on eosinophils. In contrast to the former cytokines, IL-4 is only chemotactic for eosinophils from the peripheral blood of patients with atopic dermatitis and not for eosinophils from normal individuals. IL-4 has the same chemotactic potency as the other cytokines. The optimal chemotactic potency is reached at a concentration of 10 nM. In contrast, neutrophils do not respond chemotactically to IL-4. Checkerboard analysis, inhibition studies with monoclonal anti-IL-4 antibodies, and desensitization experiments indicated specific interaction of IL-4 with eosinophils. In eosinophils from normal individuals, IL-4 responsiveness could be induced by pretreatment of the cells with IL-5 and GM-CSF. In addition to the fact that IL-4 may be responsible for selective eosinophil transendothelial migration, IL-4 may exert an important modulatory mode of action on eosinophil migration and function within allergically inflamed tissue. Our findings suggest the presence of a functional IL-4R on eosinophils from atopic dermatitis patients.
Dubois
G R
GR
Department of Dermatology/Allergology, University Hospital Utrecht, The Netherlands.
Bruijnzeel-Koomen
C A
CA
Bruijnzeel
P L
PL
D000328:Adult; D002630:Chemotactic Factors / Q000378:metabolism / Q000502:physiology; D002633:Chemotaxis / Q000187:drug effects* / Q000502:physiology; D003876:Dermatitis, Atopic / Q000097:blood* / Q000378:metabolism / Q000503:physiopathology; D004804:Eosinophils / Q000166:cytology* / Q000473:pathology* / Q000502:physiology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007377:Interleukin-3 / Q000378:metabolism / Q000494:pharmacology; D015847:Interleukin-4 / Q000378:metabolism / Q000494:pharmacology*; D015848:Interleukin-5 / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D013601:T-Lymphocytes / Q000166:cytology / Q000473:pathology / Q000502:physiology
D002630:Chemotactic Factors; D007377:Interleukin-3; D015848:Interleukin-5; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor