title: Antigenicity and immunogenicity of P30-derived peptides in experimental models of toxoplasmosis.
abstract: P30, also referred to as SAG-1, is now recognized as a major Toxoplasma gondii antigen potentially important for both diagnosis and immunoprophylaxis of toxoplasmosis. By using predictive algorithms, five synthetic peptides (48-67, 82-102, 213-230, 238-256 and 279-285) derived from P30, were investigated for B- and T-cell determinants in mouse and rat experimental models. Antibody recognition appeared more broadly distributed along the P30 sequence, whereas T-cell recognition was mainly targeted on the 238-256 peptide. In the absence of any carrier protein, this peptide induced a B- and T-cell immune response independent of the route of immunization (oral route or subcutaneous injection). This peptide (238-256) induced multiple antibody isotypes. In contrast with the 238-256 peptide, the 48-67 peptide, either free or in the form of a multiple antigenic peptide (MAP) construct or the 279-295 peptide, elicited antibodies associated with a TH2 response. This study reports for the first time the analysis of the antigenic and immunogenic properties of P30-derived peptides and are potentially useful for vaccinal strategies incorporating the P30 Toxoplasma gondii antigen.
authors.affiliation: Centre d'Immunologie et de Biologie Parasitaire, INSERM U167, CNRS 624, Institut Pasteur de Lille, France.
authors.forename: I
authors.initials: I
authors.lastname: Godard
authors.forename: J
authors.initials: J
authors.lastname: Estaquier
authors.forename: L
authors.initials: L
authors.lastname: Zenner
authors.forename: M
authors.initials: M
authors.lastname: Bossus
authors.forename: C
authors.initials: C
authors.lastname: Auriault
authors.forename: F
authors.initials: F
authors.lastname: Darcy
authors.forename: H
authors.initials: H
authors.lastname: Gras-Masse
authors.forename: A
authors.initials: A
authors.lastname: Capron
mesh_terms: D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007118:Immunoassay / Q000379:methods; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma