Interaction of fibronectin (FN) cell binding fragments and interleukin-8 (IL-8) in regulating neutrophil chemotaxis.
This study investigated the possible interaction of FN fragments in regulating IL-8-mediated neutrophil chemotaxis in vitro using Neuroprobe microchambers. Human neutrophil suspensions were incubated with purified FN fragments or an RGD-containing peptide and allowed to migrate in response to chemotactically active concentrations of human recombinant IL-8. The 120-kD fragment of FN containing the RGD sequence or an RGD peptide (GRGDSP) inhibited IL-8-mediated neutrophil chemotaxis; however, these RGD peptides did not inhibit neutrophil chemotaxis in response to other chemotactic agents. Furthermore, FN fragments not containing the RGD sequence had no effect on IL-8-mediated chemotaxis. These data suggest that directed migration of neutrophils in response to IL-8 is inhibited in the presence of cell-binding fragments of FN and may represent a local mechanism for terminating neutrophil migration at areas of tissue injury.
Gudewicz
P W
PW
Department of Physiology and Cell Biology, Albany Medical College, New York 12208.
Heinel
L A
LA
Stanton
K
K
Singleton
D
D
Frewin
M B
MB
D000595:Amino Acid Sequence; D001665:Binding Sites; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005353:Fibronectins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000166:cytology*; D009842:Oligopeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
D005353:Fibronectins; D016209:Interleukin-8; D009842:Oligopeptides; D010446:Peptide Fragments; D011994:Recombinant Proteins; C047981:arginyl-glycyl-aspartic acid