[Intratesticular subcapsular assay, a simple method for the preliminary screening of male contraceptives].
The MB-50 protocol worked out for long by the World Health Organization has been widely employed for the screening of male contraceptives. With this method, 40-80 rats will be used for 1 sample and the course of study will be about 3 months. In view of the high expenses and long duration of time needed, it seems to be not suitable for a preliminary screening. We have exploited a much simpler method, the intratesticular subcapsular assay. The method is as follows: Puncture the scrotal skin and the tunica albuginea at the equatorial plane of the testis just opposite to the epididymis with an intradermal needle fixed at a tuberculin syringe. Then push on the needle for 3-4 mm along the equator parallel to the tunica albuginea. Inject 100 microliters of solution containing 0.1-5 mg of the sample to each testis. To the controls, only the vehicle is injected. One week later the same procedure is repeated and 2 more weeks later, the animals are sacrificed and the data are observed (see Table 1 and 2). In this paper, 7 samples screened with this method were compared with results of the MB-50 method, no positive sample was missed except one false positive sample. These results indicate the coincidence between these two methods.
Xu
Y
Y
Jiangsu Family Planning Institute, Nanjing.
Wang
Y
Y
Zhang
Z T
ZT
Lin
N
N
Qian
S Z
SZ
D000818:Animals; D003272:Contraceptive Agents, Male / Q000008:administration & dosage* / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology; D004822:Epididymis / Q000187:drug effects; D005298:Fertility / Q000187:drug effects; D006072:Gossypol / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000187:drug effects; D029757:Tripterygium
D003272:Contraceptive Agents, Male; D004365:Drugs, Chinese Herbal; D006072:Gossypol