Impaired Ca2+ response to glucose in mouse beta-cells infected with coxsackie B or Echo virus.
Five strains of Coxsackie B4 virus and one of Echo 11 virus were tested with regard to their ability to replicate in pancreatic mouse beta-cells and interfere with the alterations of the cytoplasmic Ca2+ concentration ([Ca2+]i) induced by glucose. All strains except one both multiplied and caused cytopathic effect. In a control group 68% of the beta-cells responded to 11 mM glucose with large amplitude oscillations of [Ca2+]i. After inoculation with the infectious strains these oscillations appeared in only 5% of the beta-cells, whereas the non-infectious strain did not modify the glucose effect on [Ca2+]i. Despite the virus interference with the glucose response, [Ca2+]i was increased after depolarization with excessive extracellular K+ and the oscillations were induced in most beta-cells when glucose was combined with the insulin-releasing sulfonylurea tolbutamide.
Frisk
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Department of Medical Virology, Uppsala University, Sweden.
Grapengiesser
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Diderholm
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H
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000378:metabolism; D029822:Enterovirus B, Human / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism / Q000821:virology*; D051379:Mice; D014044:Tolbutamide / Q000494:pharmacology; D014779:Virus Replication
D014044:Tolbutamide; D005947:Glucose; D002118:Calcium