Linkage analysis does not support a role for glucokinase gene in the aetiology of type 2 diabetes mellitus among north western Italians.
The contribution of a 3' glucokinase gene polymorphism to the aetiology of type 2 diabetes mellitus was studied in 17 diabetic pedigrees from North-Western Italy; linkage methodology was used. A CA repeat sequence was employed as a marker and amplified by PCR. Three alleles were found: Z (195 bp), Z + 4 (199 bp) and Z + 10 (205 bp). Since in diabetic families linkage analysis gave values of LOD score between -0.000438 and 0.026, the association between GK polymorphism and type 2 diabetes could not be either excluded or accepted. Based on these data, we conclude that glucokinase polymorphism is not a major determinant of type 2 diabetes mellitus, at least in our population, but, consistent with LOD score obtained, in some pedigrees it could assume a minor role in the aetiology of this disease.
Laurino
C
C
Department of Endocrinology and Metabolism, University of Genova, Italy.
Bertolini
S
S
Cordera
R
R
D000483:Alleles; D001483:Base Sequence; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D008040:Genetic Linkage; D005838:Genotype; D005941:Glucokinase / Q000235:genetics*; D006801:Humans; D007558:Italy; D008126:Lod Score; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D005941:Glucokinase