title: A possible role for the 60-kD Ro autoantigen in a discard pathway for defective 5S rRNA precursors.
abstract: The Ro autoantigen is a 60-kD protein that is usually found in small cytoplasmic RNA-protein complexes known as Ro RNPs. Although the Ro RNPs are abundant and conserved components of a variety of vertebrate and invertebrate cells, their function is unknown. We have discovered that the Ro protein is also found complexed with certain variant 5S rRNAs in Xenopus oocytes. These RNAs contain one or more point mutations compared with the major oocyte 5S rRNA sequence as well as additional nucleotides at the 3' end. We demonstrate that the Ro protein binds specifically mutant 5S rRNAs containing 3' terminal extensions. These mutant RNAs are processed inefficiently to mature 5S rRNA and most eventually are degraded. The observation that the Ro autoantigen specifically associates with defective 5S rRNA precursors suggests that this protein may function as part of a novel quality control or discard pathway for 5S rRNA production.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: C A
authors.initials: CA
authors.lastname: O'Brien
authors.forename: S L
authors.initials: SL
authors.lastname: Wolin
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000378:metabolism*; D001327:Autoimmune Diseases / Q000097:blood; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D009865:Oocytes / Q000502:physiology; D010053:Ovary / Q000502:physiology; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000378:metabolism*; D012341:RNA, Ribosomal, 5S / Q000096:biosynthesis*; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000378:metabolism*; D014158:Transcription, Genetic; D014981:Xenopus; D000099207:SS-A Antigen
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D017931:DNA Primers; D018076:DNA, Complementary; D012322:RNA Precursors; D012341:RNA, Ribosomal, 5S; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human