title: Spin trapping of superoxide in aqueous solutions of fresh and aged cigarette smoke.
abstract: Superoxide generation in aqueous solutions of cigarette smoke was determined as a function of the age of smoke using spin trapping. As a spin trap, 5,5-dimethylpyrroline-N-oxide (DMPO) was used. The superoxide adduct of DMPO was detected in a solution of fresh main-stream smoke for over 1 h. The superoxide-generating potential of smoke was rapidly lost as the smoke was kept in a plastic syringe. The smoke that was aged for 3 min did not generate superoxide. Additional evidence of superoxide generation in aqueous solutions of cigarette smoke was obtained by the chemiluminescence method.
authors.affiliation: First Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tanigawa
authors.forename: T
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Naito
authors.forename: M
authors.initials: M
authors.lastname: Kondo
mesh_terms: D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D012906:Smoke / Q000032:analysis*; D012907:Smoking; D012996:Solutions; D013113:Spin Labels; D013481:Superoxides / Q000032:analysis*; D013997:Time Factors; D014867:Water
chemical_list: D003497:Cyclic N-Oxides; D012906:Smoke; D012996:Solutions; D013113:Spin Labels; D014867:Water; D013481:Superoxides; C017245:5,5-dimethyl-1-pyrroline-1-oxide