title: An amplified DNA element in Leishmania encodes potential integral membrane and nucleotide-binding proteins.
abstract: LD1 is a 27.5-kb sequence that occurs in an approx. 2.2-Mb chromosome in all species and strains of Leishmania. In Leishmania infantum MHOM/BL/67/ITMAP263, LD1 is also present as an inverted dimeric repeat in multicopy, 55-kb circular molecules. Sequence analysis of a 7873-nt segment derived from the circular DNA reveals 4 open reading frames (ORFs) with potential protein coding function. One ORF predicts a protein with an ATP/GTP binding site motif. Another ORF predicts a protein with 10-12 potential membrane-spanning domains, suggesting that it encodes an integral membrane protein. This protein also has homology with that predicted by the ESAG10 gene of Trypanosoma brucei.
authors.affiliation: Seattle Biomedical Research Institute, WA 98109-1651.
authors.forename: P J
authors.initials: PJ
authors.lastname: Myler
authors.forename: M J
authors.initials: MJ
authors.lastname: Lodes
authors.forename: G
authors.initials: G
authors.lastname: Merlin
authors.forename: T
authors.initials: T
authors.lastname: de Vos
authors.forename: K D
authors.initials: KD
authors.lastname: Stuart
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004270:DNA, Circular / Q000235:genetics; D016054:DNA, Protozoan / Q000032:analysis*; D005784:Gene Amplification; D006160:Guanosine Triphosphate / Q000378:metabolism; D018314:Leishmania infantum / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004270:DNA, Circular; D016054:DNA, Protozoan; D008565:Membrane Proteins; D009711:Nucleotides; D015800:Protozoan Proteins; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate