Structural investigation of the capsular polysaccharide of Escherichia coli O101 : K103 : H- using bacteriophage degradation and NMR spectroscopy.
NMR spectroscopy was performed on the depyruvated capsular antigen of E. coli K103 and on the oligosaccharide obtained by depolymerisation of the native polysaccharide with a viral-borne endoglycanase. This capsular polysaccharide is the only one to be co-expressed with O group 101 and joins a small group of unusual capsular polysaccharides which possess pyruvic acid as the only acidic function. The primary structure was shown to be composed of the repeating unit: [formula: see text]
Grue
M R
MR
School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
Parolis
H
H
Parolis
L A
LA
D016667:Bacterial Capsules / Q000737:chemistry* / Q000378:metabolism; D001435:Bacteriophages / Q000378:metabolism*; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure