Multiple peptide synthesis on acid-labile handle derivatized polyethylene supports.
Using the multipin peptide synthesis approach, a range of peptides with native amide and carboxylate C-termini were generated using an acid-labile approach. Polyethylene crowns grafted with hydroxyethylmethacrylate (HEMA) polymer were functionalized with either 4-hydroxymethylphenoxyacetic acid for the generation of peptide-carboxylate or p-[(R,S)-alpha-[1-(9H-fluoren-9-yl)methoxyformamido]-2,4-dim ethoxy- benzyl]phenoxyacetic acid for peptide-amide. A range of known peptide hormone sequences and other peptides with native C-termini were assembled by sequential incorporation of N alpha-Fmoc protected amino acids. Peptides were sidechain deprotected and cleaved from crowns with TFA/scavengers within 2 mL centrifuge tubes, and isolated by a series of ether/petrol wash and centrifugation steps. In this way it was possible to avoid a cleavage and isolation botteneck, allowing rapid processing of large numbers of peptides.
Valerio
R M
RM
Chiron Mimotopes Pty. Ltd., Clayton, Victoria, Australia.
Bray
A M
AM
Maeji
N J
NJ
D000595:Amino Acid Sequence; D002625:Chemistry, Organic / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D011095:Polyethylenes / Q000737:chemistry; D013237:Stereoisomerism
D010455:Peptides; D011095:Polyethylenes