Purification of type 1 protein (serine/threonine) phosphatases by microcystin-Sepharose affinity chromatography.
A microcystin (MC)-Sepharose column was prepared by addition of 2-aminoethanethiol to the alpha, beta-unsaturated carbonyl of the N-methyldehydroalanine residue of MC-LR, followed by reaction of the introduced amino group with N-hydroxysuccinimide-activated CH-Sepharose. The MC-Sepharose bound protein phosphatase-1 (PP1) with high capacity and purified human PP1 gamma in one step from E. coli extracts. It was also used to purify forms of PP1 bound to myofibrils from skeletal muscle. Two of these comprised PP1 complexed to N-terminal fragments of the M-subunit which enhance its myosin phosphatase activity, while the third comprised PP1 and an N-terminal fragment of the glycogen-binding (G)-subunit.
Moorhead
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G
Department of Biochemistry, University of Dundee, Scotland, UK.
MacKintosh
R W
RW
Morrice
N
N
Gallagher
T
T
MacKintosh
C
C
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli; D052998:Microcystins; D018482:Muscle, Skeletal / Q000201:enzymology; D010456:Peptides, Cyclic; D010749:Phosphoprotein Phosphatases / Q000302:isolation & purification*; D054645:Protein Phosphatase 1; D011817:Rabbits; D011994:Recombinant Proteins / Q000302:isolation & purification; D012685:Sepharose
D052998:Microcystins; D010456:Peptides, Cyclic; D011994:Recombinant Proteins; C078588:microcystin; D012685:Sepharose; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1