[Effects of panaxadiol saponins isolated from Panax notoginseng on intracellular and extracellular calcium].
Panaxadiol saponins (PDS) contain saponins Panax notoginseng B1 and E. The spontaneous beating induced by isoproterenol in isolated rat right atria and the increase of contractile force induced by Ca2+ in isolated guinea pig colon were inhibited by PDS 75 and 150 micrograms.ml-1, respectively and perhexiline 6.25 and 12.5 mumol.L-1, respectively. It suggested that PDS could block the potential-dependent and receptor-operated calcium channel in smooth muscle. PDS 150 micrograms.ml-1 and perhexiline 6.25 mumol.L-1 depressed the contractile force induced by norepinephrine and Ca2+ in isolated rat aortic strips in Ca(2+)-free Krebs' solution. It suggested that PDS and perhexiline not only inhibited the release of intracellular Ca2+ but also blocked the inflow of extracellular Ca2+.
Dan
H X
HX
Department of Pharmacology, School of Basic Medical Sciences, Beijing Medical University, China.
Zhang
B H
BH
Xie
S R
SR
Yao
J A
JA
Jia
J N
JN
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D002118:Calcium / Q000037:antagonists & inhibitors*; D015220:Calcium Channels / Q000187:drug effects*; D003106:Colon / Q000187:drug effects; D005260:Female; D036145:Ginsenosides; D006168:Guinea Pigs; D006339:Heart Rate / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D051381:Rats; D014315:Triterpenes / Q000494:pharmacology*
D015220:Calcium Channels; D036145:Ginsenosides; D014315:Triterpenes; C004564:panaxadiol; D002118:Calcium