Effect of hydroxyurea on 2',3'-dideoxycytidine activation.
The effect of hydroxyurea (HU) on cell growth, cell cycle progression, and the intracellular accumulation of 2',3'-dideoxycytidine (ddC) and ddCTP formation was examined. Twenty-four-hour exposure of H9 cells (human lymphocytic cell line) to 500 microM HU significantly increased the cells in the G1 phase and inhibited cell growth to 33.3 +/- 10.6% of control. The total intracellular ddC and ddCTP accumulation in HU-treated cells increased to 232.3% and 310% of control, respectively.
Agarwal
R P
RP
Department of Medical Oncology (R-71), University of Miami School of Medicine, FL 33101.
He
J
J
D001711:Biotransformation; D002460:Cell Line; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D016047:Zalcitabine / Q000493:pharmacokinetics*
D016047:Zalcitabine; D006918:Hydroxyurea