title: Peptide YY immunoneutralization inhibits meal-induced absorption in vivo.
abstract: BACKGROUND
  Plasma peptide YY (PYY) levels rise after a meal and have recently been shown to increase small bowel-absorption. The purpose of this study was to determine whether immunoneutralization of PYY would block postprandial absorption in vivo.
  
  
  METHODS
  Exteriorized, neurovascularly intact jejunal and ileal segments (25 cm) were created in six mongrel dogs. After a 2-week recovery luminal perfusion with an isotonic buffer, containing [14C]-polyethylene glycol as a volume marker, was used to analyze water and sodium flux after an oral meal. Each meal was accompanied by either intravenous anti-PYY (0.5 mg.kg-1.h-1) or nonspecific immunoglobulin IG (control). PYY antibody binding was determined by radioimmunoassay.
  
  
  RESULTS
  Displacement studies showed complete PYY neutralization. In control experiments feeding increased absorption of sodium and water in both segments. PYY immunoneutralization had no effect on jejunal absorption but significantly diminished ileal absorption (p < 0.05).
  
  
  CONCLUSIONS
  These results suggest that PYY acts selectively in the ileum to increase postprandial fluid and electrolyte absorption after a meal. Agents directed at PYY-stimulated absorption may prove to be of therapeutic benefit in patients with malabsorptive conditions.
authors.affiliation: Department of Surgery, University of California at Los Angeles.
authors.forename: A J
authors.initials: AJ
authors.lastname: Bilchik
authors.forename: O J
authors.initials: OJ
authors.lastname: Hines
authors.forename: S W
authors.initials: SW
authors.lastname: Ashley
authors.forename: T E
authors.initials: TE
authors.lastname: Adrian
authors.forename: J
authors.initials: J
authors.lastname: Walsh
authors.forename: H
authors.initials: H
authors.lastname: Wong
authors.forename: C D
authors.initials: CD
authors.lastname: Liu
authors.forename: M J
authors.initials: MJ
authors.lastname: Zinner
authors.forename: D W
authors.initials: DW
authors.lastname: McFadden
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D005502:Food; D005768:Gastrointestinal Hormones / Q000502:physiology*; D016756:Immunoglobulins, Intravenous / Q000276:immunology; D007408:Intestinal Absorption; D019894:Peptide YY; D010455:Peptides / Q000276:immunology / Q000502:physiology*
chemical_list: D005768:Gastrointestinal Hormones; D016756:Immunoglobulins, Intravenous; D010455:Peptides; D019894:Peptide YY