title: Improved separation of diastereomeric derivatives of enantiomers by a physical network of linear polyvinylpyrrolidone applied as pseudophase in capillary zone electrophoresis.
abstract: Diastereomeric analytes were separated using capillary zone electrophoresis with polyvinylpyrrolidone as polymeric additive to the buffer solution. As test substances derivatives of D- and L- alpha-amino and alpha-hydroxy acids formed by reaction with (+)-O,O'-diacetyl- and (+)-O,O'-dibenzoyl-L-tartaric anhydride, respectively, were used. The physical network formed by the linear polymer is supposed to act as a kind of pseudophase. It was found that the network affects the mobility of diastereomeric compounds to a different extent, enhancing the selectivity of the system. In nearly all cases of aromatic acids the diastereomer carrying the D-amino acid was more strongly retained than the L-isomer, as opposed to the situation with aliphatic acids.
authors.affiliation: Istituto di Cromatografia, Consiglio Nazionale delle Ricerche, Monterotondo Scalo Rome.
authors.forename: W
authors.initials: W
authors.lastname: Schützner
authors.forename: G
authors.initials: G
authors.lastname: Caponecchi
authors.forename: S
authors.initials: S
authors.lastname: Fanali
authors.forename: A
authors.initials: A
authors.lastname: Rizzi
authors.forename: E
authors.initials: E
authors.lastname: Kenndler
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000812:Anhydrides; D004586:Electrophoresis / Q000379:methods*; D007202:Indicators and Reagents; D015394:Molecular Structure; D011205:Povidone / Q000737:chemistry*; D012996:Solutions; D013237:Stereoisomerism; D013644:Tartrates
chemical_list: D000596:Amino Acids; D000812:Anhydrides; D007202:Indicators and Reagents; D012996:Solutions; D013644:Tartrates; C059721:O,O-diacetyl tartaric acid anhydride; D011205:Povidone