Mass spectrometric detection of preproenkephalin A-derived peptides in bovine pituitary.
A new analytical system, including chromatography separation, enzyme digestion, radioimmunoassay detection, and mass spectrometry characterization, has been designed to detect native preproenkephalin A-derived peptides in the bovine pituitary. The direct evidence of these intact peptides was provided by the mass spectrometric detection of the (M+H)+ ions of ME-Lys at m/z 702 and of ME-Arg at m/z 730, following trypsin digestion and the detection of ME-like immunoreactivity in HPLC fractions.
Yan
L
L
Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee, Memphis 38163.
Tseng
J L
JL
Desiderio
D M
DM
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004745:Enkephalins / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010902:Pituitary Gland / Q000737:chemistry*; D011498:Protein Precursors / Q000032:analysis* / Q000737:chemistry; D011863:Radioimmunoassay; D014357:Trypsin
D004745:Enkephalins; D010446:Peptide Fragments; D011498:Protein Precursors; C033660:preproenkephalin; D014357:Trypsin