title: Eradication of caseous lymphadenitis in sheep with the help of a newly developed ELISA technique.
abstract: The purpose of the study was to attempt to eradicate caseous lymphadenitis in sheep with the help of serological monitoring. A newly developed double-antibody sandwich ELISA and the culling of positive or doubtful reactors was the basis of the disease control regimen. Seropositive pregnant ewes were kept in quarantine and removed after lambing; their lambs were artificially fed and allowed to remain in the herd. Under this regimen, supplemented by a number of hygienic measures, caseous lymphadenitis was eradicated from two large flocks of sheep in which the disease was endemic.
authors.affiliation: Central Veterinary Institute-DLO, The Netherlands.
authors.forename: B E
authors.initials: BE
authors.lastname: Schreuder
authors.forename: E A
authors.initials: EA
authors.lastname: ter Laak
authors.forename: D P
authors.initials: DP
authors.lastname: Dercksen
mesh_terms: D000818:Animals; D003354:Corynebacterium Infections / Q000175:diagnosis / Q000517:prevention & control / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis; D004196:Disease Outbreaks / Q000517:prevention & control / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D008199:Lymphadenitis / Q000175:diagnosis / Q000517:prevention & control / Q000662:veterinary*; D009426:Netherlands / Q000453:epidemiology; D011247:Pregnancy; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000517:prevention & control*