Pax-QNR/Pax-6, a paired box- and homeobox-containing gene expressed in neurons, is also expressed in pancreatic endocrine cells.
After differential screening of a cDNA library constructed from quail neuroretina cells infected with the v-myc containing avian retrovirus MC29, we have isolated a cDNA clone Pax-QNR, homologous to the murine Pax6 which is mutated in the autosomal dominant mutation small eye (Sey) of the mouse and aniridia in man. Here we report the characterization of Pax-QNR/Pax-6 expression in the chicken, quail, and mouse pancreas. In situ hybridization performed with E3 chick embryos demonstrated that, in addition to the documented expression of Pax-QNR/Pax-6 in the neural tube, this gene is also expressed in the pancreatic bud. This expression is later restricted to discrete parts of the organ. From bacterially expressed Pax-QNR peptides we obtained rabbit antisera (paired domain, serum 11; domain between paired and homeo, serum 12; homeodomain, serum 13; and carboxyl-terminal part, serum 14) capable of specifically recognizing Pax-QNR/Pax-6 proteins (48, 46 kilodaltons) in cell lines derived from alpha- and beta-pancreatic cells, but not from exocrine derived cell lines. We conclude that Pax-QNR/Pax-6 represents another gene expressed both in the endocrine pancreas and neuro-ectodermic tissues.
Turque
N
N
Centre National de la Recherche Scientifique CNRS EP 56 Institut Pasteur de Lille, France.
Plaza
S
S
Radvanyi
F
F
Carriere
C
C
Saule
S
S
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002461:Cell Line, Transformed; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics; D003370:Coturnix / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D005799:Genes, Dominant; D005801:Genes, Homeobox; D018398:Homeodomain Proteins; D007106:Immune Sera; D007515:Islets of Langerhans / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D051379:Mice / Q000235:genetics / Q000254:growth & development; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009474:Neurons / Q000378:metabolism*; D009928:Organ Specificity; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D010190:Pancreatic Neoplasms / Q000473:pathology; D010446:Peptide Fragments / Q000276:immunology; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins; D012160:Retina / Q000166:cytology; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D007106:Immune Sera; D009363:Neoplasm Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; D010446:Peptide Fragments; D012097:Repressor Proteins