cDNA cloning and expression of Cry j II the second major allergen of Japanese cedar pollen.
We have isolated and sequenced a cDNA clone coding for Cry j II, the second major allergen of Japanese cedar (Cryptomeria japonica) pollen. A 1.7-kilobase cDNA clone contained an open reading frame coding for a 514-amino acid protein, including a putative signal peptide of 54 amino acid and three potential glycosylation sites. The deduced amino acid sequence of the Cry j II shows significant identities to those of the polygalacturonases associated with fruit-ripening in tomato (40%) and avocado (43%) and found in pollen of maize (34%). Cry j II cDNA product expressed in E. coli was recognized by human IgE from patients suffering from cedar pollinosis, suggesting that recombinant Cry j II might be used as an allergen for the clinical diagnosis of cedar pollinosis.
Komiyama
N
N
Meiji Institute of Health Science, Kanagawa, Japan.
Sone
T
T
Shimizu
K
K
Morikubo
K
K
Kino
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli; D015723:Gene Library; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007564:Japan; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D011058:Pollen; D012016:Reference Values; D012220:Rhinitis / Q000097:blood / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D014197:Trees / Q000235:genetics*
C065291:Cry j II protein, Cryptomeria japonica; D015345:Oligonucleotide Probes; D010940:Plant Proteins; D007073:Immunoglobulin E