title: Lipid hydroperoxide-induced peroxidation and turnover of endothelial cell phospholipids.
abstract: The effects of lipid peroxidation on rabbit aortic endothelial cell phospholipid turnover was studied using linoleic acid hydroperoxide (LOOH). Following treatments with 20-40 microM LOOH, cells prelabeled with either arachidonic acid (20:4) or oleic acid (18:1) showed a movement of these fatty acids out of the phospholipids and into neutral lipid and free fatty acid pools. There was also a release of radioactive free fatty acids and phospholipids into the media, which was significantly increased as compared to cells maintained under standard culture conditions. Fatty acid uptake and distribution among phospholipid pools was also affected by LOOH treatment where incorporation of 20:4 and 18:1 into phosphatidylcholine (PC) decreased, while uptake into phosphatidylinositol (PI) increased after 1 h of incubation with 40 microM LOOH. These effects were also inhibited by vitamin E. In cells prelabeled with 20:4 or 18:1 under conditions where approximately 99% of the fatty acids were incorporated into neutral and phospholipid pools, LOOH treatment produced a decrease in radioactivity associated with PC, while the specific activity of PI increased. The extent of these changes was greater for 20:4 than 18:1, but in each case the effects were inhibited by vitamin E. The temporal pattern of uptake for labeled choline and inositol after LOOH treatments paralleled those found for fatty acid incorporation. These cell responses indicate that induction of lipid peroxidation produces rapid fatty acid release and phospholipid turnover involving repair as well as de novo synthesis. The implications of these effects on turnover of specific phospholipids and cell responses to oxidative stress are discussed.
authors.affiliation: University of Southern California, Department of Molecular Pharmacology & Toxicology, School of Pharmacy, Los Angeles 90033.
authors.forename: E H
authors.initials: EH
authors.lastname: Pacifici
authors.forename: L L
authors.initials: LL
authors.lastname: McLeod
authors.forename: A
authors.initials: A
authors.lastname: Sevanian
mesh_terms: D000818:Animals; D001011:Aorta; D016718:Arachidonic Acid / Q000378:metabolism; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D002855:Chromatography, Thin Layer; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D008041:Linoleic Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000494:pharmacology*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D011865:Radioisotope Dilution Technique; D014316:Tritium; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D002250:Carbon Radioisotopes; D005230:Fatty Acids, Nonesterified; D008041:Linoleic Acids; D008054:Lipid Peroxides; D009829:Oleic Acids; D010743:Phospholipids; D014316:Tritium; D014810:Vitamin E; C008285:linoleic acid hydroperoxide; D016718:Arachidonic Acid; D019301:Oleic Acid; D002794:Choline