Age related effects of lectins on GAG metabolism in cultured embryonic fibroblasts.
Lectins (WGA, SBA or PNA) were administered to 7 and 14 days chick embryo fibroblasts to evaluate their ability to influence GAG cellular and extracellular accumulation. HA and sulphated GAG were differently affected by lectins. WGA addition to culture medium enhanced HA and sulphated GAG in the two examined compartments. SBA and PNA increased sulphated GAG only in the cellular pool with a consequent reduction of secreted GAG. As far as HA is concerned, SBA and PNA promoted its accumulation in the extracellular compartment. NH4Cl administration indicated a decline of GAG degradation in the course of development in presence of lectins. Generally the effect of lectins was greater in the youngest stage: showing that lectins were able to bind 7 and 14 days-old fibroblasts differently.
Becchetti
E
E
Dipartimento di Medicina Sperimentale, Università di Perugia, Italy.
Locci
P
P
Marinucci
L
L
Lilli
C
C
Pezzetti
F
F
Carinci
P
P
D000375:Aging / Q000378:metabolism*; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015683:Gene Products, gag / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology*; D013461:Sulfur Oxides / Q000494:pharmacology
D015683:Gene Products, gag; D037102:Lectins; D013461:Sulfur Oxides; D000643:Ammonium Chloride; D005944:Glucosamine