[Identification of membrane proteins of sarcoplasmic reticulum binding 2,4,6-trinitrobenzene sulfonate].
Sarcoplasmic reticulum was treated with 2,4,6-trinitrobenzene sulfonate (TNBS), which binds covalently only with surface amino groups. After solubilization of the membranes and gel-filtration of the SR protein on the Sephadex G-100, the label was discovered in the protein fraction 100-80 and 70-60 kDa. The amino groups of proteins with molecular weight 50-20 kDa are inaccessible for the 2,4,6-TNBS. Incorporation of the latter into proteins increases in presence of KCl.
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D005591:Chemical Fractionation; D002850:Chromatography, Gel; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D011485:Protein Binding; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D012995:Solubility; D014302:Trinitrobenzenesulfonic Acid / Q000378:metabolism*
D008565:Membrane Proteins; D014302:Trinitrobenzenesulfonic Acid
Identifikatsiia belkov membran sarkoplazmaticheskogo retikuluma, sviazyvasiushchikh 2,4,6-trinitrobenzolsul'fonat.