Sequence and activity of the rat PB-inducible aldehyde dehydrogenase promoter.
The 5' flanking region of the rat PB inducible aldehyde dehydrogenase gene was isolated and the sequence from +42 to -1339 was determined. This sequence contains several putative binding sites for the liver-enriched factors HNF3 and DBP as well as a GRE and several possible AP1 sites. A TATA and CCAAT motif were assigned at positions -26 and -53. A promoter construct containing the -1339 bp of the aldehyde dehydrogenase 5' flanking region was active when transfected into both H411 and HepG2 liver cell lines.
Shaw
P M
PM
Department of Cell Biology, New York University School of Medicine, New York, NY 10016.
Adesnik
M
M
D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010634:Phenobarbital / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D004247:DNA; D000444:Aldehyde Dehydrogenase; D010634:Phenobarbital