Localization of mRNAs for calpain and calpastatin in the adult rat brain by in situ hybridization histochemistry.
The detailed localization of mRNAs for calpain II and calpastatin was examined in adult rat brain by in situ hybridization histochemistry. The expression patterns of the two mRNAs were similar to each other throughout the brain in terms of relative expression intensity, and almost all neurons expressed both mRNAs more or less. Among them, neurons in cranial nerve nuclei and some others in the brain stem expressed at relatively high levels, suggesting the high involvement of the non-lysosomal proteolytic system in the function of these neurons. On the other hand, the expression levels of the two mRNAs in non-neuronal cells including glia were basically low with the choroid plexuses expressing calpastatin mRNA relatively highly.
Goto
K
K
Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan.
Iwamoto
T
T
Kondo
H
H
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000648:ultrastructure*; D001923:Brain Chemistry; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D002154:Calpain / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar
D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C033668:calpastatin; D002154:Calpain