Comparison of novel synthetic peptide-based DETECT-RUBELLA enzyme immunoassays with Enzygnost and IMx for detection of rubella-specific immunoglobulin G.
Enzyme immunoassays (EIAs) using synthetic peptides SP-E1 and SP-E1E2 (DETECT-RUBELLA [Bio-Chem]) were compared with two viral lysate-based EIAs (Enzygnost [Behring] and IMx [Abbott]) for the detection of rubella virus-specific immunoglobulin G antibodies. Sensitivities of 94.7, 100, 98.6, and 100% and specificities of 100, 97.4, 100, and 73.7% were found for the SP-E1, SP-E1E2, Enzygnost, and IMx EIAs, respectively.
Department of Microbiology and Immunology, Université de Montréal, Québec.
L
L
Pedneault
M
M
Zrein
L
L
Robillard
F
F
Landry
M
M
Lacroix
J
J
Joncas
D000914:Antibodies, Viral / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D007074:Immunoglobulin G / Q000032:analysis*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D012409:Rubella / Q000276:immunology*; D012412:Rubella virus / Q000276:immunology*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D010455:Peptides