Production of 26-deoxymonensins A and B by Streptomyces cinnamonensis in the presence of Metyrapone.
Metyrapone, a potent cytochrome P-450 inhibitor, added at 9 mM to a submerged culture of Streptomyces cinnamonensis caused partial inhibition of total monensin biosynthesis and coproduction of new metabolites, 26-deoxymonensins A and B. The latter was isolated as its 25-O-methyl derivative. Metyrapone was simultaneously reduced to metyrapol. All of these compounds were identified by nuclear magnetic resonance spectroscopy and mass spectrometry.
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague.
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Pospísil
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Sedmera
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Havlícek
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Tax
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008797:Metyrapone / Q000031:analogs & derivatives / Q000378:metabolism; D008985:Monensin / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D011725:Pyridines / Q000378:metabolism / Q000494:pharmacology*; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D011725:Pyridines; C060472:26-deoxymonensin A; C088001:26-deoxymonensin B; C002196:metyrapol; C013109:metapyrone; D008985:Monensin; D008797:Metyrapone