Regulation of herpes simplex virus thymidine kinase in cells treated with a synergistic antiviral combination of alpha interferon and acyclovir.
Alpha interferon (IFN-alpha) and acyclovir (ACV) are synergistic in their anti-herpes simplex virus activities. IFN-alpha treatment reduced the herpes simplex virus thymidine kinase (TK) activity present in cells 6 h postinfection, while steady-state levels of TK mRNA remained at or above the amount in infected, untreated cells. The inhibition of TK production by IFN-alpha treatment appeared to be transient and translational, not transcriptional.
Department of Microbiology, Medical College of Wisconsin, Milwaukee 53226.
J L
JL
Taylor
P
P
Tom
J
J
Guy
R M
RM
Selvarajan
W J
WJ
O'Brien
D000212:Acyclovir / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004357:Drug Synergism; D018259:Herpesvirus 1, Human / Q000201:enzymology*; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D008163:Luminescent Measurements; D014176:Protein Biosynthesis / Q000187:drug effects; D012367:RNA, Viral / Q000096:biosynthesis; D011994:Recombinant Proteins; D013937:Thymidine Kinase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000096:biosynthesis
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D012367:RNA, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C044200:glycoprotein H, herpes simplex virus type 1; D013937:Thymidine Kinase; D000212:Acyclovir