The inflammatory and proliferative response of normal skin in a model for acute chemical injury: ornithine decarboxylase induction as a common feature in various models for acute skin injury.
Application of sodium dodecyl sulphate (SDS) on the skin of healthy volunteers was used as a model for acute chemical injury. The time course of the response with respect to cell proliferation was studied using ornithine decarboxylase (ODC) activity. Erythema, polymorphonuclear leucocyte (PMN) infiltration, and the induction of epidermal antiproteinase activity (SKALP/elafin) were used as markers for the inflammatory response. ODC induction was similar to that in other models of acute skin injury, such as tape-stripping and ultraviolet light radiation. The amount of PMN infiltration correlated with erythema, but not with ODC induction. In contrast with findings in the tape-stripping model, no induction of SKALP/elafin activity was found after SDS application. We conclude that cell proliferation as measured by ODC induction is a common feature in the various models for skin injury. Both the kinetics and the intensity of the inflammatory response, and the induction of epidermal antiproteinase activity, appear to vary, depending on the specific model.
Department of Dermatology, University Hospital, Nijmegen, The Netherlands.
W M
WM
Van Duijnhoven-Avontuur
J A
JA
Alkemade
J
J
Schalkwijk
P D
PD
Mier
P G
PG
Van der Valk
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002057:Burns, Chemical / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004790:Enzyme Induction / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D009504:Neutrophils / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D010196:Pancreatic Elastase / Q000378:metabolism; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors / Q000378:metabolism; D012867:Skin / Q000201:enzymology / Q000473:pathology*; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage
D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors; D012967:Sodium Dodecyl Sulfate; D010196:Pancreatic Elastase; D009955:Ornithine Decarboxylase