N-methyl-D-aspartate receptor-induced translocation of protein kinase C to the nucleus in rat cerebellar slices.
Rat cerebellar slices were incubated in absence and presence of N-methyl-D-aspartate and then used to prepare a purified nuclear fraction. The purity of the nuclear fraction was assessed by electron microscopy and measurements of Na+, K(+)-ATPase activity. The presence of protein kinase C in nuclear fractions was measured by [3H]phorbol dibutyrate binding. Treatment of cerebellar slices with N-methyl-D-aspartate caused a significant, two-fold increase in the density of nuclear [3H]phorbol dibutyrate binding sites, indicating the translocation of protein kinase C to the nuclear fraction. The effect of N-methyl-D-aspartate was prevented by the presence of the N-methyl-D-aspartate receptor antagonist (+)5-methyl-10,11-dihydro-5H-dibenzocyclohepten-5,10-imine maleate (MK-801). These results suggest a possible role for protein kinase C in mediating N-methyl-D-aspartate-induced nuclear events.
Fidia-Georgetown Institute for the Neurosciences, Georgetown University School of Medicine, Washington, DC 20007.
R
R
Raulli
H
H
Alho
J T
JT
Wroblewski
D000818:Animals; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D002531:Cerebellum / Q000648:ultrastructure*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D008854:Microscopy, Electron; D016202:N-Methylaspartate / Q000494:pharmacology; D009685:Nuclear Envelope / Q000378:metabolism; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D016194:Receptors, N-Methyl-D-Aspartate; D015240:Phorbol 12,13-Dibutyrate; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D011493:Protein Kinase C; D000254:Sodium-Potassium-Exchanging ATPase