Developing human lung and kidney are major sites for synthesis of bone morphogenetic protein-3 (osteogenin).
Bone morphogenetic protein-3 (BMP-3; osteogenin) is a member of the transforming growth factor-beta superfamily. We used human BMP-3 cDNA probes and a specific BMP-3 polyclonal peptide antibody to analyze BMP-3 expression and synthesis in human fetal and adult tissues. Northern blot hybridization revealed two mRNA species of 7 and 3 KB. High levels of BMP-3 mRNA were found in fetal lungs. By in situ hybridization, the BMP-3 transcripts were found in lung bronchial epithelium, straight collecting kidney tubules, intestinal mucosa, perichondrium, periosteum, and osteoblasts of human embryos of 6-14 weeks' gestation. Cellular BMP-3 immunostaining co-localized with the distribution of RNA. In addition, bone matrix showed intensive BMP-3 staining. These data suggest that although BMP-3 has been isolated from bone matrix, it may have additional regulatory roles in the morphogenesis and/or function of human lung, kidney, and intestine.
Vukicevic
S
S
Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
Helder
M N
MN
Luyten
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FP
D000328:Adult; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D005314:Embryonic and Fetal Development / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism; D008168:Lung / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism; D014176:Protein Biosynthesis; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis
C525757:BMP3 protein, human; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D015347:RNA Probes; D012333:RNA, Messenger