Differential action of staphylococcal alpha-toxin on young and old erythrocytes.
Band 3 of rabbit erythrocytes is a binding site for staphylococcal alpha-toxin. Young erythrocytes have a functionally intact band 3 of 100 kD that is hydrolysed as the cells age into a 35-kD fragment. Consequently, aging of erythrocytes renders them more resistant to the action of the toxin. Thus the age of a blood sample will reduce the sensitivity of the hemolytic assay and contribute to the variation of this assay.
Department of Biological Sciences, University of Windsor, Ontario, Canada.
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Kantor
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Fackrell
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000187:drug effects*; D001427:Bacterial Toxins / Q000494:pharmacology*; D002499:Centrifugation, Density Gradient; D004905:Erythrocyte Aging / Q000187:drug effects*; D004906:Erythrocyte Count / Q000187:drug effects; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D007521:Isocitrate Dehydrogenase / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D011770:Pyruvate Kinase / Q000097:blood; D011817:Rabbits
D001457:Anion Exchange Protein 1, Erythrocyte; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin; D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D011770:Pyruvate Kinase