[Clinical evaluation on the detection of rifampicin specific IgG, IgM].
Using RFP-BSA as antigen, an ELISA was established to detect RFP specific IgG/IgM in the serum of 128 RFP treated patients. The results showed that RFP specific IgG/IgM could not be detected either in tuberculosis patients or healthy controls as long as the subjects were RFP free. A small number of positive results (8%) could be found in the RFP treated patients without reactions. In RFP treated patients with untoward reactions, RFP specific IgG/IgM had a high positive rate (39.3%, P < 0.05). The reactions of the positive serum could be inhibited significantly by the addition of RFP, RFP-RSA but not by RSA. In 7 patients that RFP rechallenge test was performed the result of RFP Specific-IgG/IgM was in concordance with clinical test. The results showed that this method was specific for the detection of RFP specific IgG/IgM and at least a part of RFP adverse reactions may have some relation with RFP specific IgG/IgM.
Department of Dermatology, 3rd Hospital, Beijing Medical University.
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Li
D000293:Adolescent; D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D012293:Rifampin / Q000009:adverse effects / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000276:immunology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012293:Rifampin