Inhibition of topoisomerase II by a novel antitumor cyclic depsipeptide, BE-22179.
BE-22179, a novel cyclic depsipeptide antibiotic having two 3-hydroxyquinoline moieties, inhibited the DNA-relaxing activity of L1210 topoisomerase II completely at 0.08 microM. This effect was far stronger than that of VP-16. However, it did not show any marked effect on topoisomerase II-mediated DNA cleavage. BE-22179 was ineffective in inhibiting the DNA relaxation by topoisomerase I at concentrations up to 10 microM, but showed DNA-intercalating ability (DNA unwinding) at 30 microM. The structure of BE-22179 is quite novel for a topoisomerase II inhibitor. Echinomycin, a quinoxaline antibiotic structurally related to BE-22179, interfered with DNA relaxation by topoisomerase II, though the effect was not due to inhibition of the catalytic activity of topoisomerase II but to conformational change of DNA based on its intercalation into DNA. Therefore, the potent inhibitory activity on topoisomerase II might not be a common activity of quinoxaline antibiotics, but might rather be specific to BE-22179. BE-22179 prevented DNA synthesis as well as RNA synthesis in L1210 cells and inhibited the growth of the cells. However, it remains unclear to what extent the topoisomerase II inhibition was responsible for the cytotoxicity of BE-22179.
Banyu Tsukuba Research Institute.
T
T
Yoshinari
H
H
Okada
A
A
Yamada
D
D
Uemura
H
H
Oka
H
H
Suda
A
A
Okura
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004264:DNA Topoisomerases, Type I / Q000187:drug effects; D004448:Echinomycin / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology; D007939:Leukemia L1210 / Q000201:enzymology*; D009690:Nucleic Acid Conformation; D010455:Peptides; D059005:Topoisomerase II Inhibitors; D014407:Tumor Cells, Cultured
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D010455:Peptides; D059005:Topoisomerase II Inhibitors; C086645:BE 22179; D004448:Echinomycin; D005047:Etoposide; D004264:DNA Topoisomerases, Type I