Possibility of signal transduction through microfilaments below the membrane following ligand-receptor interaction.
In this paper, we describe the changes of microfilament assembly and 3H-TdR incorporation in mouse ascites liver cancer cells under the action of concanavalin A (ConA) and laminin (LN). We have also studied the variation of 3H-TdR incorporation induced by destroying microfilaments with cytochalasin B (CB) following ConA and LN binding with their membrane receptors. It was found that ConA and LN interactions with their membrane receptors could induce the assembly of microfilaments below the membrane and promote DNA synthesis in these cells, but this effect was inhibited when microfilaments were destroyed by CB treatment. These results suggest that microfilaments might play a role in transferring signals from the membrane to the nucleus.
Department of Cell Biology, Beijing Medical University.
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Wei
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Cai
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Liu
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Tao
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Su
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D007797:Laminin / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology; D051379:Mice; D011952:Receptors, Concanavalin A / Q000187:drug effects; D017474:Receptors, Laminin / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D007797:Laminin; D011952:Receptors, Concanavalin A; D017474:Receptors, Laminin; D003208:Concanavalin A; D003571:Cytochalasin B