Ethyl alcohol reduces cardiac output, stroke volume, and end diastolic volume in the embryonic chick.
It has been established that ethanol causes both human congenital cardiac malformations and structural intracardiac abnormalities in the embryonic chick. In view of a theory that reduced embryonic tissue hemodynamics are associated with the development of malformations, we attempted to determine whether or not a) ethanol altered cardiac blood flow and b) altered hemodynamics were a function of ethanol dose in the chick embryo. Cardiac function in Hamburger-Hamilton stage 19 chick embryos was recorded on videotape before and up to 10 hours after exposure to graded doses of ethanol. Parameters of cardiac function, including cardiac output, were determined from videotaped images by means of computer assistance. Cardiac output decreased in a linear fashion with dose for up to 3 hours after exposure to ethanol. The maximum relative percent decrease in cardiac output was directly related to the dose of ethanol administered. Furthermore, the time required after ethanol treatment for mean cardiac output to return to pretreatment and control values was also dose-dependent--lower doses of ethanol required less time for mean cardiac output to return to pretreatment and control values. Although relatively high doses of ethanol depress cardiac rate, we attribute the significant decrease in cardiac output primarily to parallel dose-dependent decreases in both stroke volume and end diastolic volume. Our data are consistent with the hypothesis that reduced embryonic cardiac blood flow during cardiogenesis is associated with the development of ethanol-induced intracardiac defects in chick embryos.
School of Pharmacy, University of Wyoming, Laramie 82071.
H J
HJ
Bruyere
C E
CE
Stith
D000818:Animals; D002303:Cardiac Output, Low / Q000139:chemically induced*; D002642:Chick Embryo / Q000187:drug effects* / Q000502:physiology; D003326:Coronary Circulation / Q000187:drug effects; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology / Q000633:toxicity*; D006321:Heart / Q000187:drug effects / Q000196:embryology* / Q000503:physiopathology; D006339:Heart Rate / Q000187:drug effects; D013318:Stroke Volume / Q000187:drug effects*
D000431:Ethanol