The phylogenetic position of Hydrogenobacter acidophilus based on 16S rRNA sequence analysis.
Hydrogenobacter acidophilus strain 3H-1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen-oxidizer isolated from a Japanese solfataric field. Strain 3H-1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H-1, and sequenced the amplification product directly. Phylogenetic analyses show strain 3H-1 is closely related to Aquifex pyrophilus and may be located in the deepest branch within the eubacterial phylogenetic tree. Sulfur-dependency of the ancestral eubacterium is also discussed.
Department of Biotechnology, Central Research Institute of Electric Power Industry, Chiba, Japan.
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Shima
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Yanagi
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Saiki
D001105:Archaea / Q000145:classification / Q000235:genetics; D001483:Base Sequence; D005051:Eubacterium / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry* / Q000235:genetics
D012336:RNA, Ribosomal, 16S