Luffin-S--a small novel ribosome-inactivating protein from Luffa cylindrica. Characterization and mechanism studies.
We purified and characterized a novel RIP (ribosome inactivating protein), Luffin-S from the seeds of Luffa cylindrica. Different from Luffin-A and B, which are RNA N-glycosidases with molecular weights of 27 and 28 kDa, respectively, Luffin-S has an M.W. of only approx. 10 kDa, much smaller than any other RIPs so far investigated. Its abundant resources, toxicity similar to TCS in a cell-free protein synthesis system and unique mechanism as phosphodiesterase, like alpha-sarcin, promisingly make it a potential toxic moiety of immunotoxin.
Shanghai Institute of Biochemistry, Academia Sinica, P.R. China.
W
W
Gao
J
J
Ling
X
X
Zhong
W
W
Liu
R
R
Zhang
H
H
Yang
H
H
Cao
Z
Z
Zhang
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002474:Cell-Free System; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects; D006358:Hot Temperature; D008970:Molecular Weight; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors; D012335:RNA, Ribosomal / Q000378:metabolism; D011817:Rabbits; D012259:Ribonuclease, Pancreatic / Q000032:analysis / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects*; D012639:Seeds / Q000737:chemistry*
D000596:Amino Acids; D005656:Fungal Proteins; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012335:RNA, Ribosomal; C014801:alpha-sarcin; D004722:Endoribonucleases; D012259:Ribonuclease, Pancreatic; D010727:Phosphoric Diester Hydrolases