Myelination of regenerated optic fibers in peripheral nerve graft of adult cats.
Retinal ganglion cells of adult cats have the potential to regenerate their axons into autografted peripheral nerve. Two months after transplantation of the sciatic nerve to the axotomized optic stump, regenerated axons were labeled anterogradely with biocytin, and myelin formation by Schwann cells was examined electron microscopically. Both myelinated and unmyelinated fibers were labeled with biocytin. Among 511 axons labeled in three grafts, 96 fibers (18.8%) were myelinated and 415 (81.2%) were unmyelinated. Mean diameter with SD of myelinated fibers was 1.28 +/- 0.39 micron (range 0.71-2.47) and that of unmyelinated fibers was 0.76 +/- 0.38 micron (range 0.18-2.46). The ratio of inner to outer diameters of the myelin sheath (g value) was 0.82, which is close to the value (0.8) for the optic fibers of intact adult cats.
Watanabe
M
M
Department of Physiology, Institute for Developmental Research, Aichi Colony, Kasugai, Japan.
Sawai
H
H
Fukuda
Y
Y
D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D002415:Cats; D017690:Cell Transplantation / Q000502:physiology*; D006735:Horseradish Peroxidase; D008239:Lysine / Q000031:analogs & derivatives; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000502:physiology* / Q000648:ultrastructure; D009412:Nerve Fibers / Q000502:physiology* / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology*; D009900:Optic Nerve / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D017933:Peripheral Nervous System / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000502:physiology* / Q000648:ultrastructure; D012583:Schwann Cells / Q000502:physiology / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000502:physiology / Q000637:transplantation / Q000648:ultrastructure
D006735:Horseradish Peroxidase; C013411:biocytin; D008239:Lysine