A gene-sized DNA molecule encoding the catalytic subunit of DNA polymerase alpha in the macronucleus of Oxytricha nova.
We have isolated a gene-sized molecule encoding the catalytic subunit of DNA polymerase alpha from a macronuclear genomic library of Oxytricha nova, by using a 0.7-kb fragment of the corresponding human gene as a hybridization probe. Two different versions of the gene are present in the macronucleus, one with an EcoRI site (RI+) and one without an EcoRI site (RI-). The cloned RI- version has been characterized. It is 4938 bp in length, excluding telomeres. It consists of a 329-bp 5' leader, a 4479-bp coding region and a 130-bp 3' trailer. The deduced amino-acid sequence shares conserved regions with the yeast and human polypeptides. We also demonstrate by Southern analysis that gene-sized molecules of similar size, homologous to the isolated O. nova gene are present in the mac genome of closely and distantly related hypotrichs.
Mansour
S J
SJ
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347.
Hoffman
D C
DC
Prescott
D M
DM
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D005075:Biological Evolution; D002467:Cell Nucleus / Q000201:enzymology*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004257:DNA Polymerase II / Q000235:genetics*; D016054:DNA, Protozoan; D006801:Humans; D008969:Molecular Sequence Data; D016813:Oxytricha / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
D016054:DNA, Protozoan; D004257:DNA Polymerase II