Molecular analysis of the F plasmid traVR region: traV encodes a lipoprotein.
The nucleotide sequences of the conjugative F plasmid transfer region genes, traV and traR, have been determined. The deduced amino acid sequence of TraV indicated that it may be a lipoprotein; this was confirmed by examining the effect of globomycin on traV-encoded polypeptides synthesized in minicells. An open reading frame that may represent a previously undetected transfer gene, now designated trbG, was identified immediately upstream of traV. The deduced product of traR was found to share amino acid similarity with proteins from the bacteriophages 186 and P2 and with the dosage-dependent dnaK suppressor DksA.
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
T J
TJ
Doran
S M
SM
Loh
N
N
Firth
R A
RA
Skurray
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001435:Bacteriophages / Q000737:chemistry; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005144:F Factor / Q000235:genetics*; D008074:Lipoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; C080512:TraR protein, Agrobacterium tumefaciens; C088022:TraV protein, E coli; D014764:Viral Proteins