Expression of a calcium binding protein pEL98 (mts1) during differentiation of human promyelocytic leukemia HL-60 cells.
The expression of pEL98 (also termed mts1), an S100-related calcium-binding protein, was induced during macrophagic or granulocytic differentiation of human promyelocytic leukemia HL-60 cells in response to phorbol 12-myristate 13-acetate or dimethylsulfoxide, respectively. However, pEL98 expression remained at the low level during granulocytic differentiation of the cells by the treatment with all-trans retinoic acid. The expression of cell motility, but not adhesiveness or phagocytic ability, was found to be coincided with that of pEL98 (mts1), suggesting that pEL98 (mts1) is involved in regulating cell motility. Immunocytochemical analysis demonstrated that pEL98 (mts1) was expressed in human monocytes, macrophages and polymorphonuclear leukocytes.
Division of Chemotherapy, Chiba Cancer Center Research Institute, Japan.
K
K
Takenaga
Y
Y
Nakamura
S
S
Sakiyama
D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015870:Gene Expression; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*; D010587:Phagocytosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C077591:S100A4 protein, human; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide