Evaluation of two commercial systems for identification of coagulase-negative staphylococci to species level.
Bloodstream (224) and urine (nine) isolates of coagulase-negative staphylococci (CNS) were evaluated using MicroScan Pos ID and Rapid Pos ID panels. A modification of the conventional method of Kloos and Schleifer served as the reference method. The isolates were selected to include a broad range of CNS species, including 44 S. epidermidis, 50 S. hominis, 39 S. warneri, 33 S. capitis, 21 S. haemolyticus, 12 S. simulans, 11 S. saprophyticus, six S. cohnii, five S. lugdunensis, three S. xylosus, four S. auricularis, two S. schleiferi, two S. intermedius, and one S. sciuri. The Pos ID panel had an overall rate of agreement (correct plus probably correct) with the reference method of 79%, including 95% for S. epidermidis, 95% for S. haemolyticus, 64% for S. hominis, 67% for S. simulans, 79% for S. warneri, and 100% for S. saprophyticus. The Rapid Pos ID panel had an overall rate of agreement (correct plus probably correct) of 76%, including 91% for S. epidermidis, 90% for S. haemolyticus, 64% for S. hominis, 58% for S. simulans, 77% for S. warneri, and 100% for S. saprophyticus. Both systems are acceptable for the identification of the clinically significant species S. haemolyticus, S. saprophyticus, and S. epidermidis, but are less reliable for the infrequently isolated species of CNS.
Grant
C E
CE
Department of Pathology, Oregon Health Sciences University, Portland.
Sewell
D L
DL
Pfaller
M
M
Bumgardner
R V
RV
Williams
J A
JA
D016470:Bacteremia / Q000175:diagnosis; D015373:Bacterial Typing Techniques; D001437:Bacteriuria / Q000175:diagnosis; D003030:Coagulase / Q000378:metabolism; D005069:Evaluation Studies as Topic; D006801:Humans; D011933:Reagent Kits, Diagnostic; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification
D003030:Coagulase; D011933:Reagent Kits, Diagnostic