A rapid method for the preparation of ganglioside Glac2 (GD3).
A method is described for the preparation of ganglioside Glac2 [(II3(NeuAc)2-LacCer, GD3] from cream of bovine milk using liquid-phase extraction with methanol or ethanol followed by anion exchange chromatography. The method is rapid and inexpensive; 1 kg cream, centrifuged from 14-15 L of bovine milk, yields approximately 70 mg of pure ganglioside Glac2. The sialic acid constituent of ganglioside Glac2 isolated from bovine milk cream consists solely of the N-acetylneuraminic acid derivative. The major components of its ceramide consist of octadecasphing-4-enine and the 22:0 (behenic acid) and 23:0 fatty acids.
Jennemann
R
R
Physiologisch-Chemisches Institut, Philipps-Universität, Marburg, Germany.
Wiegandt
H
H
D000818:Animals; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000737:chemistry; D002240:Carbohydrate Sequence; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods; D002855:Chromatography, Thin Layer; D003611:Dairy Products; D000431:Ethanol; D005260:Female; D005732:Gangliosides / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D000432:Methanol; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012794:Sialic Acids / Q000032:analysis; D013045:Species Specificity
D005732:Gangliosides; D012794:Sialic Acids; D000431:Ethanol; C026226:ganglioside, GD3; D000432:Methanol