A p12 gag gene homologue is present in the mouse genome.
A replication-defective virus (BM5d) of approximately 4.9 kb, is responsible for a retrovirus induced immunodeficiency syndrome in mice (MAIDS) that shares many features with AIDS. BM5d is characterized by deletions in env and pol genes, furthermore its gag gene differs markedly from gag of other BM5 ecotropic viruses, particularly in its p12 sequence. The p12 region of the gag gene has been shown to account for the pathogenicity of the BM5d retrovirus. During our studies of BM5d integration in mice we found that p12-like sequences are present in the mouse genome of uninfected healthy C57BL/6 mice. Cloning and sequencing of this p12 gag homologue has revealed a high (63% to 89%) amino acid derived sequence identity with other retroviruses and shown that the major differences among p12 of pathogenic viral strains compared to non-pathogenic ones consist of a four amino acids deletion and a high abundance of proline and basic amino acids in their p12 region.
Institute of Biological Chemistry G. Fornaini, University of Urbino, Italy.
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Casabianca
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Magnani
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D003673:Defective Viruses / Q000235:genetics; D015683:Gene Products, gag / Q000737:chemistry / Q000235:genetics*; D015750:Genes, gag; D016678:Genome; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016183:Murine Acquired Immunodeficiency Syndrome / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D015683:Gene Products, gag; D004247:DNA