An optical biosensor based upon glucose oxidase immobilized in sol-gel silicate matrix.
Glucose oxidase immobilized in a transparent silicate gel prepared by a sol-gel method was used as a simple solid-state optical biosensor for glucose. The sensor was based upon the measurement of initial rates of reduction of the FAD prosthetic group of the enzyme in the presence of various concentrations of glucose. The analytical range of the sensor was 1-100 mM, and the measurement time was 2 min. The enzyme was considerably protected by the silicate matrix against leaching, thermal inactivation and even H2O2-dependent inactivation. The sensor was stable in daily use for 6 months.
Shtelzer
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A. Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel.
Braun
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D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors / Q000737:chemistry*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D055095:Optics and Photonics; D010084:Oxidation-Reduction; D058428:Silica Gel; D012822:Silicon Dioxide; D013379:Substrate Specificity; D013696:Temperature
D004800:Enzymes, Immobilized; D005182:Flavin-Adenine Dinucleotide; D058428:Silica Gel; D012822:Silicon Dioxide; D005949:Glucose Oxidase; D005947:Glucose