An aroA homologue from Synechocystis sp. PCC 6803.
In this study, we report the entire nucleotide sequence of an aroA homologue encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), isolated from the cyanobacterium Synechocystis sp. PCC 6803. The proposed coding region is an open reading frame of 447 amino acids. The deduced sequence of the gene product is particularly similar to the Gram+ EPSPS sequences available to date, in particular to that in Bacillus subtilis. Analysis of the Synechocystis putative EPSPS sequence does not lead to an obvious explanation for the natural tolerance of this cyanobacterium to glyphosate.
Dalla Chiesa
M
M
Department of Biochemistry, Wolfson Laboratories, Imperial College of Science Technology and Medicine, London, UK.
Mayes
S R
SR
Maskell
D J
DJ
Nixon
P J
PJ
Barber
J
J
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D014166:Transferases / Q000737:chemistry / Q000235:genetics*
D004269:DNA, Bacterial; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase