PE-1, a novel ETS oncogene family member, localizes to chromosome 1q21-q23.
The v-ets oncogene family shares a conserved peptide motif called the ETS domain that mediates sequence-specific DNA binding. This motif is unique among transcription factor families. Using partially degenerate oligonucleotides from conserved regions of the ETS domain and the polymerase chain reaction, we isolated a new member of the v-ets family designated PE-1 from HL60 cells. PE-1 was expressed as an approximately 7.5-kb transcript in most cell lines tested. In the hairy cell leukemia line Eskol, there was an additional 1.8-kb transcript observed. PE-1 was the most common ETS domain gene found in CD34+HLA-DR- hematopoietic progenitors. PE-1 was localized to human chromosome 1q21-q23 using both in situ chromosomal hybridization and human-hamster hybrids.
Department of Microbiology/Immunology, Indiana University Medical Center, Indianapolis 46202.
M
M
Klemsz
R
R
Hromas
W
W
Raskind
E
E
Bruno
R
R
Hoffman
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D015513:Oncogene Proteins / Q000737:chemistry / Q000235:genetics*; D009857:Oncogenes; D016133:Polymerase Chain Reaction; D050783:Proto-Oncogene Proteins c-ets; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C088164:ETV3 protein, human; D015513:Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D015682:Retroviridae Proteins, Oncogenic; C050379:oncogene proteins v-ets