Banded krait minor-satellite (Bkm)-associated Y chromosome-specific repetitive DNA in mouse.
The mouse Y chromosome remains highly condensed in all somatic tissues but decondenses extensively in testis. We have isolated a mouse Y chromosome-specific repeat M34 (11.5 kb) and shown that this is distributed along the Y chromosome except the sex-determining region (the Y short arm) in which GATA repeats are predominantly concentrated. It has 32 copies of GATA repeats in a 2.7 kb fragment. About 200-300 copies of M34 on the Y chromosome are interspersed among other sequences. A 1.2 kb fragment (p3) of M34, containing GATA repeats, also has scaffold attachment region (SAR) motifs which bind to nuclear matrices. A strong affinity of histone H1 to SAR motifs is implicated in maintaining the condensed state of the Y chromosome in somatic tissues. The probable significance of molecular organization of the Y chromosome is discussed.
Centre for Cellular and Molecular Biology, Hyderabad, India.
L
L
Singh
S G
SG
Panicker
R
R
Nagaraj
K C
KC
Majumdar
D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000378:metabolism*; D005260:Female; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014998:Y Chromosome
D004276:DNA, Satellite