Autologous bone marrow purging with LAK cells.
In this study we will demonstrate that LAK cells, in vitro, can lyse hematologic neoplastic cells with a minor toxicity of the staminal autologous marrow cells. In fact, after bone marrow and LAK co-culture at a ratio of 1/1 for 8 hours, the inhibition on the GEMM colonies resulted to be 20% less compared to the untreated marrow. These data made LAK an inviting agent for marrow purging in autologous bone marrow transplantation.
Division of Hematology, USSL 3, Pesaro, Italy.
L
L
Giuliodori
L
L
Moretti
S
S
Stramigioli
F
F
Luchetti
G M
GM
Annibali
A
A
Baldi
D016465:Bone Marrow Purging / Q000379:methods; D016026:Bone Marrow Transplantation; D002455:Cell Division; D002478:Cells, Cultured; D015925:Cryopreservation; D003602:Cytotoxicity, Immunologic; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology / Q000276:immunology; D007938:Leukemia / Q000628:therapy; D008223:Lymphoma / Q000628:therapy; D014182:Transplantation, Autologous