Accumulation of amyloid beta-protein precursor (APP) in Purkinje cells and increase of amino-terminal fragments of APP in cerebrum and cerebellum of aged rat brain.
In aged rat brain, amyloid beta-protein precursor (APP) is accumulated in dendrites and cell bodies of Purkinje cells as full-length or truncated APP, because dendrites and cell bodies are positively stained by antibodies against both the amino- and carboxy-termini of APP. Western blot analysis of homogenates of brains of aged and young rats showed no apparent differences except for an increase in amino-terminal fragments in cerebrum and cerebellum of aged rat. These results indicate that the expression, transport or metabolism of APP in specific regions of brains may be affected by the aging process.
Department of Neuropsychiatry, Osaka University Medical School, Japan.
Y
Y
Nakamura
M
M
Takeda
H
H
Niigawa
F
F
Kametani
S
S
Hariguchi
I
I
Yoshida
S
S
Kitajima
T
T
Nishimura
D000375:Aging / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism; D007150:Immunohistochemistry; D010446:Peptide Fragments / Q000378:metabolism*; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D010446:Peptide Fragments