Posttreatment with amino compounds effective in prevention of calcification of glutaraldehyde treated pericardium.
An attempt has been made to compare the role of different amino compounds in prevention of calcification of glutaraldehyde (GA)-treated pericardium implanted subcutaneously in the rat for 12 weeks. GA pretreated pericardial samples were postfixed separately with glycine, albumin, gentamicin, glycine + gentamicin, and albumin + gentamicin. Severe calcification was noticed in animals undergoing albumin or gentamicin postfixation. Mild calcification was observed in glycine and albumin + gentamicin postfixed materials whereas calcium was undetectable either chemically or morphologically in GA-pretreated pericardium postfixed with glycine + gentamicin (0.32 +/- 0.13.mg/g calcium, dry wt; von Kossa 0) at the 12th week of implantation in the rat.
Chanda
J
J
Department of Cardiovascular Surgery, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
D000418:Albumins; D000818:Animals; D002118:Calcium; D002417:Cattle; D005839:Gentamicins; D005976:Glutaral; D005998:Glycine; D010496:Pericardium; D014021:Tissue Preservation
D000418:Albumins; D005839:Gentamicins; D002118:Calcium; D005976:Glutaral; D005998:Glycine