Effect of electromagnetic stimulation with different waveforms on cultured chick tendon fibroblasts.
An energy efficient electromagnetic stimulator device for fracture healing was compared to a commercially available device in stimulating cell growth in tissue cultures. The energy efficient device, which conserves energy by using a bidirectional time-dependent magnetic wave form, and the commercially available stimulator, which uses a unidirectional time-dependent magnetic wave form, were tested on chick tendon fibroblasts in primary culture. Comparing non-stimulated control and cells electromagnetically stimulated with unidirectional and bidirectional waveforms showed that at the growth phase between days 2 and 3, both electrical stimulation techniques increased cell division as measured by DNA synthesis. When cells were dividing rapidly, collagen synthesis was reduced. When the cells reached the confluence there was no difference among the groups (control, unidirectionally stimulated, and bidirectionally stimulated) in terms of number of cells or collagen produced.
Guzelsu
N
N
Biomechanics Program, S.O.M., Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
Salkind
A J
AJ
Shen
X
X
Patel
U
U
Thaler
S
S
Berg
R A
RA
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D004574:Electromagnetic Fields; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D005583:Fourier Analysis; D013710:Tendons / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*
D003094:Collagen; D004247:DNA; D000242:Cyclic AMP