The nusG gene of Streptomyces griseus: cloning of the gene and analysis of the A-factor binding properties of the gene product.
The nusG gene of Streptomyces griseus was cloned and the nucleotide sequence determined. It encodes a protein with an identity of 76% to the reported receptor (VbrA) for VB-C, an autoregulatory factor in Streptomyces virginae. NusG protein was expressed in Escherichia coli. However, no binding activity for A-factor, an butyrolactone autoregulator in S. griseus very similar to VB-C, could be detected. The nusG gene of S. griseus does not seem to encode the A-factor-binding protein.
Institut für Chemische Mikrobiologie, BUGH Wuppertal, FRG.
S
S
Kuberski
T
T
Kasberg
J
J
Distler
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010445:Peptide Elongation Factors / Q000235:genetics* / Q000378:metabolism; D013305:Streptomyces griseus / Q000235:genetics*; D014157:Transcription Factors
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C070291:NusG protein, E coli; D010445:Peptide Elongation Factors; D014157:Transcription Factors; C025035:A-factor (Streptomyces); D015107:4-Butyrolactone