The SON gene encodes a conserved DNA binding protein mapping to human chromosome 21.
We report the identification and characterization of a clone for the DNA binding protein SON, which we have isolated from a human keratinocyte cDNA library. Using this clone we have found that the SON gene is expressed in different cell types and that homologous sequences can be detected in vertebrate and insect genomic DNA. Using the polymerase chain reaction (PCR) to amplify SON sequences from a panel of somatic cell hybrids we have assigned the gene encoding human SON to chromosome 21. By use of hybrids containing regions of chromosome 21 the localization has been refined to 21q 22.1-q22.2.
Department of Biochemistry, Charing Cross and Westminster Medical School, University of London.
I M
IM
Khan
R A
RA
Fisher
K J
KJ
Johnson
M E
ME
Bailey
M J
MJ
Siciliano
A M
AM
Kessling
M
M
Farrer
B
B
Carritt
T
T
Kamalati
L
L
Buluwela
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D005796:Genes / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D015603:Keratinocytes / Q000737:chemistry*; D015778:Minor Histocompatibility Antigens; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D001616:beta-Galactosidase / Q000235:genetics
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D015778:Minor Histocompatibility Antigens; D011498:Protein Precursors; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C088203:SON protein, human; C032253:involucrin; D001616:beta-Galactosidase