[Functional activity of the nucleolar organizer in the interphase granulocytic nuclei in chronic myeloid leukemia].
Silver staining was used to investigate transcription activity of ribosomal genes of leukemia granulocytes in the interphase in 32 patients with PA-positive chronic myeloid leukemia (CML) and 26 healthy subjects. Visual calculation of nucleolar argentophilic granules (AG) and measurements of AG total area on cytoanalyzer Morphoquant were made. Introduction of a specially devised computer program and combination of silver staining with autoradiography in vitro (3H-thymidine) made it possible to quantify the activity of the ribosome genes in each phase of the mitotic cycle (G0/1, S, G2). Moreover, a significant difference was established in AgNO3 staining capacity of mature granulocyte nuclei in normal condition and in CML.
V I
VI
Petushkov
I L
IL
Strozha
D K
DK
Bondare
N A
NA
Mironova
N I
NI
Voskoboĭnik
A Ia
AIa
Ose
D001345:Autoradiography; D006098:Granulocytes / Q000166:cytology / Q000648:ultrastructure*; D006801:Humans; D007399:Interphase / Q000502:physiology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D008938:Mitosis / Q000502:physiology; D009697:Nucleolus Organizer Region / Q000502:physiology*; D012016:Reference Values; D016622:Silver Staining; D012984:Software
Funktsional'naia aktivnost' iadryshkovogo organizatora v interfaznnykh iadrakh granulotsitov pri khronicheskom mieloleĭkoze.