Ki-67 immunostaining and DNA flow cytometry as prognostic factors in epithelial ovarian cancers.
Samples from malignant epithelial ovarian tumors were examined for expression of Ki-67, DNA ploidy and S-phase fraction (SPF). In addition, normal ovary, benign and borderline tumors were immunostained with Ki-67 to examine its role in ovarian carcinogenesis and to estimate the prognostic significance. For both Ki-67, ploidy and SFP a significant correlation to survival in malignancy was observed. Furthermore, SPF above 10% showed significant correlation to decreased survival in both stage subgroups I-II and III-IV, while for aneuploidy a decrease in survival could be detected only in localized disease. Ki-67 expression was present in tumor cells in the main part of borderline and malignant tumors and even in 2 benign counterparts, which might indicate an active state in these commonly believed dormant neoplasms. In the malignant group the Ki-67 correlation to survival seemed to be independent of staining intensity, although the observed decay in survival seemed to be faster in the strongly stained group.
Department of Gynecology and Obstetrics, University Hospital, Uppsala, Sweden.
R
R
Henriksen
P
P
Strang
T
T
Backstrom
E
E
Wilander
B
B
Tribukait
K
K
Oberg
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins / Q000032:analysis*; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000401:mortality / Q000473:pathology*; D010053:Ovary / Q000166:cytology / Q000473:pathology; D011003:Ploidies; D011379:Prognosis; D012016:Reference Values; D016019:Survival Analysis; D015996:Survival Rate; D013997:Time Factors
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins