Simple and rapid high-performance liquid chromatography method for the determination of alcuronium in human plasma and urine.
A simple and quick HPLC assay for alcuronium is presented. Its characteristics are: precipitation of plasma proteins by acetonitrile; Spherisorb 5-CN column; acetonitrile-water (46:54, v/v) as mobile phase; flow-rate 1 ml/min; laudanosine 0.06 mg/l as internal standard with plasma; external standard with urine; UV detection at 294 nm; retention time 5.4 min; detection limit 0.025 mg/l; documented linearity: 0.025-2.0 mg/l for plasma and 1.0-80 mg/l for urine; intra- and inter-assay variability below 4%. None of nine drugs used in perioperative pharmacotherapy interfered with the assay. Satisfactory performance was exemplified in a 12-h pharmacokinetic evaluation of two patients.
University Department of Pharmacology, Köln, Germany.
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T
Künzer
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Buzello
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M
Theisohn
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C
Diefenbach
D000443:Alcuronium / Q000032:analysis* / Q000493:pharmacokinetics; D000758:Anesthesia; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D000443:Alcuronium