Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from Arabidopsis and a bacterial gene for beta-glucuronidase.
Production of a functional beta-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.
Molecular Genetics Research Laboratory, University of Tokyo, Japan.
T
T
Takahashi
N
N
Yabe
Y
Y
Komeda
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006358:Hot Temperature; D007700:Kinetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000378:metabolism
D006360:Heat-Shock Proteins; D005966:Glucuronidase