Involvement of gp130/interleukin-6 receptor transducing component in interleukin-11 receptor.
The recently cloned interleukin (IL)-11 displays many biological properties in common with those reported for IL-6. In order to analyze the nature and the functionality of the IL-11 receptor we developed a proliferative assay using the human multifactor-dependent cell line TF1. We showed that a blocking monoclonal antibody GPX7 raised against the gp130/IL-6 receptor transducing subunit was also able to inhibit the IL-11-triggered TF1 line proliferation. In addition, involvement of gp130 in IL-11 signaling was demonstrated by an induction of the transducing protein phosphorylation in response to IL-11, as observed for IL-6. In contrast, the blocking monoclonal antibody B-R6, which recognized the gp80/IL-6 binding subunit failed to interfere with the IL-11 proliferative signal in the TF1 cell line. Similarly, we did not observe any competition between IL-6 and IL-11 for a putative common binding site on the cell surface. These results suggest that the IL-11 binding component is different from the gp80/IL-6 receptor. In conclusion, IL-11, along with IL-6, leukemia inhibitory factor, oncostatin M and ciliary neurotrophic factor, belongs to the same family of cytokines, using gp130 as a transducing protein.
Fourcin
M
M
INSERM U 298, Laboratoire de biologie cellulaire, CHRU Angers, France.
Chevalier
S
S
Lebrun
J J
JJ
Kelly
P
P
Pouplard
A
A
Wijdenes
J
J
Gascan
H
H
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D002455:Cell Division / Q000276:immunology; D050822:Cytokine Receptor gp130; D006801:Humans; D015151:Immunoblotting; D017370:Interleukin-11 / Q000502:physiology*; D053703:Interleukin-11 Receptor alpha Subunit; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology / Q000502:physiology*; D011485:Protein Binding; D018123:Receptors, Interleukin / Q000502:physiology*; D053702:Receptors, Interleukin-11; D019947:Receptors, Interleukin-6; D015398:Signal Transduction / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C508428:IL11RA protein, human; C494199:IL6ST protein, human; D017370:Interleukin-11; D053703:Interleukin-11 Receptor alpha Subunit; D008562:Membrane Glycoproteins; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11; D019947:Receptors, Interleukin-6; D050822:Cytokine Receptor gp130