Genotypic and phenotypic relatedness of 80 strains of Branhamella catarrhalis of worldwide origin.
80 clinical Branhamella catarrhalis strains of worldwide origin were examined for genotypic relatedness and phenotypic characteristics. Using a quantitative bacterial dot method for DNA-DNA hybridization the strains were found to form a homogeneous group with delta Tm-values ranging from 0.0-2.3 degrees C, In Minibact-N, an identification kit for oxidase positive, Gram-negative diplococci using eight phenotypic characteristics, all isolates were correctly identified and also demonstrated complete homogeneity except for beta-lactamase production. Type strains representing the genera Branhamella, Moraxella and Neisseria were also examined for comparison. B. catarrhalis strain NCTC 4103-known to be atypical-had a delta Tm-value of 5.7 degrees C and produced gamma-glutamylaminopeptidase, in contrast to all other B. catarrhalis strains. In GN MicroPlate, a kit which tests utilizable carbon sources, B. catarrhalis strains were found to be able to utilize up to 16 to 95 carbon sources.
Department of Clinical Microbiology, Herlev Hospital, Denmark.
J J
JJ
Christensen
J
J
Ursing
B
B
Bruun
D002244:Carbon / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D001936:Moraxella catarrhalis / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis; D010641:Phenotype
D004269:DNA, Bacterial; D002244:Carbon; D010088:Oxidoreductases