Platelet 3H-paroxetine binding in control subjects and depressed patients: relationship to serotonin uptake and age.
3H-paroxetine is regarded as a better ligand for the serotonin (5-hydroxytryptamine; 5-HT) uptake site than 3H-imipramine. In the present study, platelet 14C-5-HT uptake and 3H-paroxetine binding were simultaneously measured in 12 control subjects. There was a significant positive correlation between the individual Bmax value for 3H-paroxetine binding and the Vmax value for 14C-5-HT uptake. Platelet 3H-paroxetine binding was also determined in 21 drug-free patients who satisfied DSM-III-R criteria for major depression and 21 control subjects. A negative correlation was found between the Bmax values for 3H-paroxetine binding with age in control subjects. There was no change in 3H-paroxetine binding in depressed patients compared with control subjects. Our results indicated that 3H-paroxetine was a good ligand for evaluating 5-HT uptake sites, and the influence of age ought to be taken into consideration in the study of 3H-paroxetine binding. The present study indicated that there was no change in 5-HT uptake sites in platelets from depressed patients.
Department of Neuropsychiatry, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
M
M
Nankai
S
S
Yamada
S
S
Yoshimoto
A
A
Watanabe
H
H
Mori
K
K
Asai
M
M
Toru
D000328:Adult; D000367:Age Factors; D001665:Binding Sites; D001792:Blood Platelets / Q000378:metabolism*; D003866:Depressive Disorder / Q000378:metabolism*; D003891:Desipramine / Q000493:pharmacokinetics; D005260:Female; D005473:Fluoxetine / Q000493:pharmacokinetics; D006801:Humans; D007099:Imipramine / Q000493:pharmacokinetics; D007223:Infant; D008297:Male; D017374:Paroxetine / Q000493:pharmacokinetics*; D012701:Serotonin / Q000378:metabolism*
D005473:Fluoxetine; D012701:Serotonin; D017374:Paroxetine; D007099:Imipramine; D003891:Desipramine