Transplacental exposure to genotoxins. Evaluation in haemoglobin of hydroxyethylvaline adduct levels in smoking and non-smoking mothers and their newborns.
The N-(2-hydroxyethyl)valine (HOEtVal) adduct resulting from the interaction of ethylene oxide with the N-terminal valine in haemoglobin, was determined in blood samples taken from non-smoking pregnant women (n = 10) and from pregnant women (n = 13) smoking 15 or more cigarettes a day. The HOEtVal levels were also determined in the haemoglobin from blood samples taken, within 48 h of delivery, from the newborns of these mothers. In the maternal haemoglobins, the average HOEtVal adduct level was increased from a background of 63 +/- 20 (mean +/- SD) pmol/g globin in the non-smoking mothers to 361 +/- 107 (mean +/- SD) pmol/g globin in the smoking mothers. Haemoglobin adducts were also detected in all the babies' blood samples. The concentrations in the newborns from smoking mothers [147 +/- 68 (mean +/- SD) pmol/g globin] was significantly higher (P < 0.01) than the concentrations in the newborns from non-smoking mothers [42 +/- 18 (mean +/- SD) pmol/g globin]. A significant correlation (P < 0.01) was found between newborns and maternal HOEtVal adduct levels.
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Portugal.
R
R
Tavares
P
P
Ramos
J
J
Palminha
M A
MA
Bispo
I
I
Paz
A
A
Bras
J
J
Rueff
P B
PB
Farmer
E
E
Bailey
D002273:Carcinogens / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000187:drug effects*; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D012907:Smoking / Q000009:adverse effects* / Q000378:metabolism*; D014633:Valine / Q000031:analogs & derivatives* / Q000378:metabolism
D002273:Carcinogens; D006454:Hemoglobins; C057442:2-hydroxyethylvaline; D014633:Valine