Immunoperoxidase staining of hepatitis C virus in formalin-fixed, paraffin-embedded needle liver biopsies.
The localization of hepatitis C virus (HCV) in the liver has not been well clarified. We report successful indirect immunoperoxidase staining of the HCV core antigen using polyclonal antibodies raised in rabbits and conventional formalin-fixed, paraffin-embedded needle biopsy sections of liver. The core antigen was distributed in a fine granular pattern diffusely, perisinusoidally, or focally within the hepatocellular cytoplasm of livers from patients with HCV infection. The staining tended to show a more heterogeneous pattern in terms of intensity and distribution in cases of more advanced disease. Hepatocellular carcinoma cells were also frequently stained. HCV immunostaining will provide important information on the pathogenesis and treatment of HCV-related liver diseases.
Department of Pathology, Nihon University School of Medicine, Tokyo, Japan.
T
T
Uchida
T
T
Shikata
E
E
Tanaka
K
K
Kiyosawa
D000328:Adult; D000368:Aged; D005260:Female; D005557:Formaldehyde; D016174:Hepacivirus / Q000276:immunology*; D006512:Hepatitis B Core Antigens / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008107:Liver Diseases / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010232:Paraffin
D006512:Hepatitis B Core Antigens; D005557:Formaldehyde; D010232:Paraffin