Tissue autofluorescence as an intermediate endpoint in NMBA-induced esophageal carcinogenesis.
The capacity to identify subclinical neoplastic disease of the upper aerodigestive tract (UADT) using tissue auto-fluorescent spectroscopy would significantly contribute to cancer screening. Rats received N-nitrosomethyl benzylamine (NMBA), a carcinogen shown to cause esophageal malignancies. Following sacrifice at early weekly intervals, gross assessment of esophageal mucosa of NMBA-exposed rats was indistinguishable from saline-treated controls. Histopathologic evaluation, however, revealed NMBA-induced preneoplastic changes in the epithelium. Concurrent with these changes, the NMBA-exposed rats demonstrated specific alterations in autofluorescence. These results demonstrate that NMBA-induced esophageal premalignancy can be distinguished by autofluorescent properties. The capacity to detect alterations in autofluorescence may allow more sensitive screening of UADT mucosa at risk for cancer development.
Glasgold
R
R
Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10021.
Glasgold
M
M
Savage
H
H
Pinto
J
J
Alfano
R
R
Schantz
S
S
D000818:Animals; D002273:Carcinogens; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology; D004128:Dimethylnitrosamine / Q000031:analogs & derivatives; D004938:Esophageal Neoplasms / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology; D005453:Fluorescence; D011230:Precancerous Conditions / Q000175:diagnosis / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013057:Spectrum Analysis
D002273:Carcinogens; C014707:nitrosobenzylmethylamine; D004128:Dimethylnitrosamine