Formation of amyloid-like substance from beta-2-microglobulin in vitro. Role of serum amyloid P component: a preliminary study.
Although the pathogenesis has yet to be fully understood, beta 2-microglobulin (beta 2m) related amyloidosis is a frequent complication in long-term hemodialysis (HD) patients. In an attempt to clarify the association of two potential candidates with amyloidogenesis from beta 2m in HD patients, human urine-derived beta 2m solution alone or combined with glycosaminoglycans: hyaluronic acid, heparan sulfate, or serum amyloid P component (SAP) were dialyzed against physiological buffered solution (pH 7.4) using a microdialyzer in vitro for 72 h at 4 degrees C. This study demonstrates for the first time that SAP can play a crucial role in the formation of amyloid-like fibrils from beta 2m. This occurs by a direct influence on either the processing of a precursor protein, or protein folding, in vitro, by a short-period dialysis against a physiological buffered solution.
Ono Geka Clinic, Fukuoka, Japan.
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Uchino
D000682:Amyloid / Q000096:biosynthesis* / Q000648:ultrastructure; D000686:Amyloidosis / Q000209:etiology; D003956:Dialysis; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D006435:Renal Dialysis / Q000009:adverse effects; D000683:Serum Amyloid P-Component / Q000378:metabolism*; D001613:beta 2-Microglobulin / Q000378:metabolism* / Q000652:urine
D000682:Amyloid; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D000683:Serum Amyloid P-Component; D001613:beta 2-Microglobulin