[A role of cytokines produced by allostimulated lymphocytes on maintenance of pregnancy--enhancement of trophoblast proliferation and beta-hCG secretion by cytokines].
The purpose of this study was to elucidate the enhancement of pregnancy maintenance by cytokines produced by allostimulated lymphocytes. Two-way mixed lymphocyte cultures between wife and husband were performed in 20 normal fertile couples and 21 unexplained recurrent aborting couples and the supernatants were harvested. Couples were defined as normally fertile if they had had two or more successful pregnancies. The effects of mixed wife-husband lymphocyte culture supernatant (MLCS) and wife's lymphocyte culture supernatant (LCS) on DNA synthesis and beta-hCG secretion of cultured human trophoblast cells were examined. 1. MLCS of normal fertile couples significantly enhanced DNA synthesis (238.2 +/- 19.9% of control, p < 0.0001) and beta-hCG secretion (190.5 +/- 8.9% of control, p < 0.001) from trophoblast cells. However, MLCS of recurrent aborting couples and LCS of both groups were observed to have no significant effect on trophoblast proliferation or beta-hCG secretion. 2. Concentrations of macrophage colony-stimulating factor (M-CSF), Interleukin (IL)-2 and IL-6 in MLCS and LCS were measured. The mean M-CSF level in MLCS of the normal fertile group was 182.0 +/- 26.1 IU/ml, in contrast, it was not detected in MLCS of recurrent aborting couples or LCS of either group. No significant difference was found between the groups in the IL-2 or IL-6 concentration. 3. Recombinant M-CSF (100 IU/ml, 250 IU/ml) significantly enhanced both DNA synthesis (191.7 +/- 15.7% of control, p < 0.05, 212.2 +/- 8.8% of control, p < 0.05) and beta-hCG secretion (144.8 +/- 13.9% of control, p < 0.02, 197.4 +/- 23.0% of control, p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Kanaya
M
M
Department of Obstetrics and Gynecology, Sapporo Medical University, School of Medicine.
Tanaka
S
S
D002455:Cell Division; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D011247:Pregnancy; D011255:Pregnancy Maintenance; D011994:Recombinant Proteins / Q000494:pharmacology; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor; D004247:DNA