Separation of closely related intrinsic membrane polypeptides of the photosystem II light-harvesting complex (LHC II) by reversed-phase high-performance liquid chromatography on a poly(styrene-divinylbenzene) column.
The three closely related intrinsic membrane polypeptides of the photosystem II light-harvesting complex (LHC II) were successfully resolved on a PRP-1 poly(styrene-divinylbenzene) column using a three-stage linear water-acetonitrile gradient containing 0.1% trifluoroacetic acid. The hydrophobic proteins of photosystem I (PS I-200) and photosystem II core particles were also separated by this method, showing that membrane proteins of different sizes and hydrophobicities can be resolved in this system.
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Botany Department, University of British Columbia, Vancouver, Canada.
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D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004591:Electrophoresis, Polyacrylamide Gel; D007475:Ion Exchange Resins; D008565:Membrane Proteins / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D011137:Polystyrenes; D013056:Spectrophotometry, Ultraviolet
D007475:Ion Exchange Resins; D008565:Membrane Proteins; D010455:Peptides; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D011137:Polystyrenes; C003771:Amberlite XAD-2 resin