Immunoglobulin-secreting cells in healthy peripheral blood mononuclear cells.
Immunoglobulin-secreting cells were measured in healthy uncultured peripheral blood mononuclear cells (PBMCs) in vitro. The percentage of IgM-, IgG- and IgA-secreting cells in adult PBMCs was 0.053, 0.099 and 0.065%, respectively. The percentage of IgM-, IgG- and IgA-secreting cells was 0.73, 5.2 and 3.8% of surface IgM-, IgG- and IgA-bearing cells, respectively. The numbers of IgM-, IgG- and IgA-secreting cells increased with age in childhood. However, the numbers of all three classes were slightly decreased in adults compared with children aged 9-15 years. These results may explain the difference in immunity in vivo.
Department of Pediatrics, Gifu University School of Medicine, Japan.
N
N
Kondo
R
R
Inoue
S
S
Shinoda
T
T
Orii
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D002648:Child; D002675:Child, Preschool; D006462:Hemolytic Plaque Technique; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007148:Immunoglobulin mu-Chains / Q000096:biosynthesis / Q000235:genetics; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008875:Middle Aged; D010950:Plasma Cells / Q000276:immunology / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology*; D012323:RNA Processing, Post-Transcriptional; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D011506:Proteins; D011947:Receptors, Antigen, B-Cell