Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.
The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced. They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grown on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in AP3. The hyb operon was mapped at 65 min on the E. coli chromosome.
Department of Biochemistry, University of Georgia, Athens 30602.
N K
NK
Menon
C Y
CY
Chatelus
M
M
Dervartanian
J C
JC
Wendt
K T
KT
Shanmugam
H D
HD
Peck
A E
AE
Przybyla
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006864:Hydrogenase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon; D010641:Phenotype; D017422:Sequence Analysis, DNA
D006864:Hydrogenase