Identification of the aromatase in the breast carcinoma cell lines T47D and MCF-7.
The presence of estrogens in tumour cells is considered to be a critical factor for the development of the hormone-dependent forms of breast cancer. The last, rate-limiting step of estrogen biosynthesis is controlled by cytochrome P-450 type enzyme complex named aromatase. In the present study we determined and characterized the expression of aromatase mRNA in the breast carcinoma cell lines T47D and MCF-7. The expression was characterized by slot blot hybridization, reverse transcriptase-polymerase chain reaction technique and Northern hybridization analysis. Northern blotting revealed the presence of 4.4 kb and 2.4 kb messengers in both cell lines.
Department of Oncology, Montreal General Hospital, McGill University, Que., Canada.
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Sadekova
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Tan
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Chow
D001141:Aromatase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000201:enzymology*; D002460:Cell Line; D005260:Female; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010920:Placenta / Q000201:enzymology; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D001141:Aromatase