The pacL gene of Synechococcus sp. strain PCC 7942 encodes a Ca(2+)-transporting ATPase.
An ATP-dependent Ca2+ uptake activity was identified in plasma membrane vesicles prepared from Synechococcus sp. strain PCC 7942. This activity was insensitive to agents which collapse pH gradients and membrane potentials but sensitive to vanadate, indicating that the activity is catalyzed by a P-type Ca(2+)-ATPase. A gene was cloned from Synechococcus sp. strain PCC 7942 by using a degenerate oligonucleotide based on a sequence conserved among P-type ATPases. This gene (pacL) encodes a product similar in structure to eukaryotic Ca(2+)-ATPases. We have shown that pacL encodes a Ca(2+)-ATPase by demonstrating that a strain in which pacL is disrupted has no Ca(2+)-ATPase activity associated with its plasma membrane. In addition, Ca(2+)-ATPase activity was restored to the delta pacL strain by introducing pacL into a second site in the Synechococcus sp. strain PCC 7942 chromosome.
Berkelman
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Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305.
Garret-Engele
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Hoffman
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NE
D001483:Base Sequence; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D017136:Ion Transport; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D014634:Valinomycin / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
D014634:Valinomycin; D014638:Vanadates; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000252:Calcium-Transporting ATPases; D002118:Calcium