Interleukin-1 and interleukin-6 modify protein phosphorylation in the central nervous system of Hirudo medicinalis.
Recent data suggest that IL-1-like molecules have been conserved during evolution. The signal transduction mechanism of IL-1 is not known, but kinase activation has been reported. With the aim of studying if human IL-1 has any effect on the leech nervous system, we have added this cytokine to segmental ganglia labeled with [32P]ortophosphoric acid; proteins have been separated by electrophoresis and phosphoproteins detected by autoradiography. In the present paper we show that human IL-1 and IL-6 are able to induce changes on protein phosphorylation in the leech central nervous system and that these changes are similar to those ones induced by the neurotransmitter serotonin.
Department of Physiology and Biochemistry G. Moruzzi, University of Pisa, Italy.
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D
Bottai
M
M
Garcia-Gil
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ML
Zaccardi
L
L
Fineschi
M
M
Brunelli
D000818:Animals; D002490:Central Nervous System / Q000187:drug effects* / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D007858:Learning / Q000187:drug effects; D007865:Leeches / Q000187:drug effects* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation; D012701:Serotonin / Q000494:pharmacology*
D007375:Interleukin-1; D015850:Interleukin-6; D009419:Nerve Tissue Proteins; D012701:Serotonin