A truncated version of an ADP-glucose pyrophosphorylase promoter from potato specifies guard cell-selective expression in transgenic plants.
ADP-glucose pyrophosphorylase (AGPase) is a key regulatory enzyme in starch biosynthesis in higher plants. A 3.2-kb promoter of the large subunit gene of the AGPase from potato has been isolated and its activity analyzed in transgenic potato and tobacco plants using a promoter-beta-glucuronidase fusion system. The promoter was active in various starch-containing cells, including guard cells, tuber parenchyma cells, and the starch sheath layer of stems and petioles. No expression was observed in mesophyll cells. Analysis of various promoter derivatives showed that with respect to expression in petioles and stems, essential elements must be located in the 5' distal region of the promoter, whereas elements important for expression in tuber parenchyma cells are located in an internal fragment comprising nucleotides from positions -500 to -1200. Finally, a 0.3-kb 5' proximal promoter fragment was identified that was sufficient to obtain exclusive expression in guard cells of transgenic potato and tobacco plants. The implications of our observations are discussed with respect to starch synthesis in various tissues and the use of the newly identified promoter as a tool for stomatal biology.
Institut für Genbiologische Forschung Berlin GmbH, Germany.
B
B
Müller-Röber
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U
La Cognata
U
U
Sonnewald
L
L
Willmitzer
D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D051599:Glucose-1-Phosphate Adenylyltransferase; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000096:biosynthesis / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics*; D016385:TATA Box
D009713:Nucleotidyltransferases; D051599:Glucose-1-Phosphate Adenylyltransferase; D005966:Glucuronidase