Staurosporine and gossypol are inhibitors of the function of peptide elongation factor 1 alpha from rabbit reticulocytes.
As it has been found, the incubation of [gamma-32P]ATP with elongation factor--1 alpha purified from rabbit reticulocytes resulted in the phosphorylation of several substrate proteins /Tuhácková, Z. (1992) In: Rec. Adv. Cell. Mol. Biol. 4, 79-86, Peeters Press, Leuven/ (1). In the present paper chromatofocusing of the purified eEF-1 alpha demonstrates that the ATP-dependent protein kinase activity is associated with a single protein catalyzing the GTP-dependent binding of aminoacyl-tRNA to ribosomes. Both of these activities are inhibited by staurosporine and gossypol. The inhibition by GDP but not by GTP indicates a possible involvement of conformation changes also in the modulation of the protein kinase activity displayed by eEF-1 alpha.
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Praha.
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Tuhácková
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006072:Gossypol / Q000494:pharmacology*; D006153:Guanosine Diphosphate / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000037:antagonists & inhibitors* / Q000097:blood / Q000302:isolation & purification; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D019311:Staurosporine
D000470:Alkaloids; D006657:Histones; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D006153:Guanosine Diphosphate; D011493:Protein Kinase C; D019311:Staurosporine; D006072:Gossypol