Chemiluminescent hybridization assay for hepatitis delta virus RNA in serum.
A nonradioactive hybridization assay for the detection of hepatitis delta virus RNA in serum is described. This assay utilizes a digoxigenin-labeled RNA hybridization probe and chemiluminescent immunodetection. The probe can detect as little as 0.4 pg of cDNA with a 60-min exposure. Results obtained are in agreement with serological indicators of hepatitis delta virus infection and are comparable to those obtained by hybridization assays employing radioactively labeled RNA.
Gallagher
M
M
Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Morris
T T
TT
Fields
H A
HA
D005069:Evaluation Studies as Topic; D003699:Hepatitis D / Q000175:diagnosis* / Q000382:microbiology; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
D015347:RNA Probes; D012367:RNA, Viral