In vivo transfection by hepatitis A virus synthetic RNA.
Marmosets injected intrahepatically with nucleic acids (cDNA and RNA transcripts) representing the full-length genome of the wild-type HM-175 strain of hepatitis A virus experienced acute hepatitis and seroconversion to hepatitis A virus capsid proteins. The hepatitis was comparable in severity to that caused by infection with the wild-type virus. The viral cDNA and the hepatitis A virus recovered from the feces of an injected animal contained the same marker mutation. Therefore, intermediate cell culture steps can be omitted and the virulence of a hepatitis A virus encoded by a cDNA clone can be evaluated by direct transfection of marmosets.
Emerson
S U
SU
National Institutes of Health, Bethesda, Maryland.
Lewis
M
M
Govindarajan
S
S
Shapiro
M
M
Moskal
T
T
Purcell
R H
RH
D000818:Animals; D002144:Callithrix; D006506:Hepatitis A / Q000235:genetics* / Q000473:pathology; D008099:Liver / Q000382:microbiology; D012367:RNA, Viral / Q000138:chemical synthesis / Q000235:genetics*; D014162:Transfection
D012367:RNA, Viral