The interaction of glycosaminoglycans with heparin cofactor II.
The binding sites for dermatan sulfate and heparin in HCII overlap but are not identical. This may explain the observation that HCII binds nonspecifically to heparin oligosaccharides, but preferentially binds to a minor hexasaccharide isolated from dermatan sulfate. The tissue distribution of dermatan sulfate molecules containing the high-affinity HCII binding site may regulate HCII activity in vivo. Finally, in the presence of dermatan sulfate or heparin, the N-terminal acidic region of HCII may interact with the hirudin-binding site of thrombin to produce maximal stimulation of the thrombin-HCII reaction.
Tollefsen
D M
DM
Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003871:Dermatan Sulfate / Q000737:chemistry; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000378:metabolism*; D015844:Heparin Cofactor II / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013917:Thrombin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014961:X-Ray Diffraction
D006025:Glycosaminoglycans; D009844:Oligosaccharides; D011994:Recombinant Proteins; D003871:Dermatan Sulfate; D015844:Heparin Cofactor II; D013917:Thrombin