The influence of long-term administration of Tołpa Peat Preparation on immune reactivity in mice. II. The effect of intermittent TPP administration on the morphological picture of lymphatic organs.
The studies were conducted on 200 Balb/c mice (inbred strain), aged 8 weeks. The mice were administered Tołpa Peat Preparation (TPP) in drinking water (10 or 50 mg/kg/day) for seven weeks (experiment I). Two lobes of the thymus, the spleen and the axillary and mesenteric lymph nodes of mice were taken for histological examinations after 3, 5 and 7 weeks of continuous TPP treatment and also 12 and 16 weeks from the beginning of the treatment. In experiment II, TPP was administered daily at the same doses for 3 weeks, and intermittently for 9 consecutive weeks; a week interval followed each seven-day cycle. The lymphatic organs of mice were taken for histological examinations after 3, 5, 7, 9 and 12 weeks of TPP treatment. It was found that daily administration of TPP (as described above) to mice for 7 weeks (10 mg/kg) induced a marked stimulation of the lymphatic organs (in the first weeks of TPP treatment) and resulted in retrograde changes (depletion of thymocytes and lymphocytes) in the lymphatic structures when TPP administration was prolonged (4-7 weeks). The morphological changes due to TPP administered at the dose of 50 mg/kg were less pronounced in the organs examined. The retrograde changes in the lymphatic structures disappeared 9 weeks after TPP treatment had been stopped. Similarly, the changes showing stimulation of the lymphatic organs were maintained throughout the entire period of 9 weeks.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Pathological Anatomy and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, Agriculture University, Wrocław, Poland.
J A
JA
Madej
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B
Obmińska-Domoradzka
T
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Garbuliński
D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000494:pharmacology; D000596:Amino Acids / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D002241:Carbohydrates / Q000008:administration & dosage* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D006812:Humic Substances / Q000008:administration & dosage* / Q000494:pharmacology; D008221:Lymphoid Tissue / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012987:Soil; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects; D014574:Uronic Acids / Q000008:administration & dosage* / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000596:Amino Acids; D002241:Carbohydrates; D004338:Drug Combinations; D006812:Humic Substances; D012987:Soil; C084029:Tolpa; D014574:Uronic Acids