Changes in neuronal metabolism in hypothalamic periventricular regions following acute alteration of cerebrospinal fluid composition in conscious rats.
Quantitative 2-deoxyglucose (2-DG) autoradiography was employed to determine the effect of elevating cerebrospinal fluid osmolality on neuronal metabolism of hypothalamic periventricular regions in conscious rats. Injection of hypertonic mannitol solution into the dorsal third ventricle had no significant effect on local cerebral glucose utilization, whereas administration into the ventral third ventricle resulted in a significant elevation in 2-DG uptake in only the posterior pituitary, median preoptic nucleus (MnPo), median eminence and suprachiasmatic nucleus. These results indicate the contribution of a synaptic connection in the MnPo in the activation of the osmoreceptor complex.
Wells
T
T
Division of Neurophysiology and Neuropharmacology, NIMR, Mill Hill, London, UK.
Achaaban
M R
MR
Richards
H K
HK
Forsling
M L
ML
D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry / Q000502:physiology; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D003847:Deoxyglucose; D005947:Glucose / Q000378:metabolism; D006982:Hypertonic Solutions; D007032:Hypothalamus, Anterior / Q000166:cytology / Q000378:metabolism*; D007276:Injections, Intraventricular; D008297:Male; D008353:Mannitol / Q000008:administration & dosage / Q000134:cerebrospinal fluid / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism*; D011301:Preoptic Area / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D006982:Hypertonic Solutions; D008353:Mannitol; D003847:Deoxyglucose; D005947:Glucose