Molecular characterization of the gene for human interleukin-1 beta converting enzyme (IL1BC).
Interleukin-1 beta (IL-1 beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor by a protease called the IL-1 beta converting enzyme (ICE). A cDNA encoding this protease was recently isolated. A human genomic clone containing the ICE gene (IL1BC) was isolated using the cDNA as a probe. The gene consists of 10 exons spanning at least 10.6 kb. 5'-anchored polymerase chain reaction indicated a single transcription start site approximately 33 bp upstream of the initiator Met codon. The 5'-flanking region does not have an apparent TATA box but may contain an initiator (Inr) promoter element. However, transcriptional activity could not be detected with a fusion gene containing the 5'-flanking region linked to the bacterial chloramphenicol acetyltransferase gene (CAT) when transfected into the human acute monocytic leukemia cell line THP-1. Using the genomic IL1BC clone, we have confirmed the localization of the gene to chromosome 11 band q22.2-q22.3 by fluorescence in situ hybridization.
Cerretti
D P
DP
Immunex Corporation, Seattle, Washington 98101.
Hollingsworth
L T
LT
Kozlosky
C J
CJ
Valentine
M B
MB
Shapiro
D N
DN
Morris
S W
SW
Nelson
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020170:Caspase 1; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D003062:Codon; D017931:DNA Primers; D015342:DNA Probes; D005091:Exons; D015698:Genomic Library; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D016385:TATA Box; D014158:Transcription, Genetic
D003062:Codon; D017931:DNA Primers; D015342:DNA Probes; D020170:Caspase 1; D008666:Metalloendopeptidases