Ubiquinone-10 protects neurons from virus-induced degeneration.
Cultured neurons from rat dorsal root ganglia and cerebral cortex were infected with Sendai virus, which gives a productive replication with lysis of most neurons, and with the RW strain of mumps virus, which undergoes defective replication causing degeneration of only 30-40% of the neurons within 5 days after initial infection. In Sendai virus-infected cells the amount of polyisoprenoid lipids was enhanced. In mumps virus-infected cultures there were transient reductions in the contents of cholesterol, dolichol, and ubiquinone-9 in the cultures, whereas the reduction in the ubiquinone-10 level was progressive, reaching 20% of its original value 21 days after infection. Treatment of mumps virus-infected cultures with ubiquinone-10 protected the neurons from degeneration, whereas no effects were observed on exposure to ubiquinone-9. Linolenic acid (18:3) and arachidonic acid (20:4), but not myristic acid (14:0) and palmitic acid (16:0), also had significant neuroprotective effects.
Edlund
C
C
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Holmberg
K
K
Dallner
G
G
Norrby
E
E
Kristensson
K
K
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000502:physiology*; D002784:Cholesterol / Q000378:metabolism; D004286:Dolichols / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000502:physiology*; D007700:Kinetics; D009109:Mumps virus / Q000472:pathogenicity / Q000502:physiology*; D009410:Nerve Degeneration / Q000187:drug effects*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010222:Parainfluenza Virus 1, Human / Q000472:pathogenicity / Q000502:physiology*; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000494:pharmacology; D013997:Time Factors; D014451:Ubiquinone / Q000378:metabolism / Q000494:pharmacology*; D014779:Virus Replication; D020122:tert-Butylhydroperoxide
D004286:Dolichols; D005230:Fatty Acids, Nonesterified; D010545:Peroxides; D017382:Reactive Oxygen Species; D014451:Ubiquinone; D020122:tert-Butylhydroperoxide; D002784:Cholesterol; C025204:Ubiquinone Q2; C030571:ubiquinone 9