Relationship between intercellular communication and adriamycin resistance in non-small cell lung cancer.
The adriamycin chemosensitivity and extent of gap junctional intercellular communication were assessed in a panel of seven human non-small cell lung cancer (NSCLC) cell lines. Communication was assessed by autoradiographic detection of transfer of 3H uridine nucleotides between coupled cells. The strength of coupling varied widely between the cell lines and they could be separated into 3 groups: those which exhibited strong coupling, L-DAN and A549; those which exhibited weak coupling, SK-MES-1, Calu-3 and NCI-H125; and an intermediate group, WIL and NCI-H23. Adriamycin chemosensitivity was assessed by both clonogenic and MTT assays. The range of IC50 values as measured by either assay was extremely narrow, with no important differences between the lines. Thus, despite the wide spectrum of intercellular communication observed in these lines, this did not correlate with their adriamycin resistance.
Cancer Research Campaign Beatson Laboratories, Beatson Institute for Cancer Research, Bearsden, Glasgow, U.K.
C
C
Bradley
R I
RI
Freshney
J
J
Pitts
D001345:Autoradiography; D002289:Carcinoma, Non-Small-Cell Lung; D002450:Cell Communication; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000633:toxicity*; D004351:Drug Resistance; D006801:Humans; D007365:Intercellular Junctions / Q000502:physiology*; D008175:Lung Neoplasms; D014316:Tritium; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay; D014529:Uridine / Q000378:metabolism*
D014316:Tritium; D004317:Doxorubicin; D014529:Uridine