Studies on bcl-2 protein in cultured lymphoid cells.
Bcl-2 protein was analysed in cell cultures derived from non-Hodgkin lymphomas and chronic lymphocytic leukaemia patients. The presence of bcl-2 proto-oncogene protein product has been demonstrated on cytospin preparations utilising immunochemical methods. The correlation between the expression of the bcl-2 protein and the appearance of proliferation- or differentiation related figures was studied. The reported bcl-2 up regulation in malignant lymphoid cells was confirmed in haemo- and lymphopoietic progenitor cells as well as in TPA/PHA activated peripheral blood lymphocytes. In comparison to neoplastic cells lower intensity of bcl-2 positive staining in freshly isolated cells from non-leukaemic tissue was observed.
Filip
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Department of Human Genetics, Medical Academy, Lublin, Poland.
Rozynkowa
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D000911:Antibodies, Monoclonal; D001853:Bone Marrow; D002455:Cell Division; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism*; D008198:Lymph Nodes; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000737:chemistry*; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry*; D011505:Protein-Tyrosine Kinases; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D019253:Proto-Oncogene Proteins c-bcl-2; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011505:Protein-Tyrosine Kinases