Continuous noninvasive measurement of in vivo pH in conscious mice.
Extracellular pH was measured continuously and noninvasively in hairless mice using a transcutaneous spectrofluorometric technique and a fluorescent pH probe, bis-carboxyethyl carboxyfluorescein (BCECF). The acid form of BCECF was injected intravenously. An optical fiber system excited the fluorescent probe at 450 and 500 nm alternately, using one branch of a bifurcated, fiber optic bundle. The other branch of the bundle collected the emitted fluorescence which was measured by a photomultiplier tube. The ratio of fluorescence intensities from the two excitation wavelengths was pH dependent. Measurements could be obtained for approximately 60 min following a single injection. Mice exposed to elevated partial pressures of CO2 demonstrated changes in the fluorescence ratio indicative of a dramatic decrease in extracellular pH. If the exposure was brief, the fluorescence signal recovered within 20 min. The fluorescence intensity ratio was calibrated against aqueous BCECF samples in vitro. Fluorescence pH values determined using the in vitro calibration were compared with measurements made on blood samples taken from seven mice. Extracellular pH in hairless mice was found to be approximately 7.5, within the expected physiological range. The variability of the pH signal derived from the fluorescence signal was approximately 0.05 units.
School of Chemical Sciences, University of East Anglia, Norwich, Norfolk, UK.
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Russell
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Pottier
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Valenzeno
D000818:Animals; D002245:Carbon Dioxide; D005110:Extracellular Space / Q000378:metabolism*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008812:Mice, Hairless; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000379:methods*; D010777:Photochemistry; D013050:Spectrometry, Fluorescence
D005452:Fluoresceins; D005456:Fluorescent Dyes; D002245:Carbon Dioxide; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein