Simple and sensitive quantitation method for mevalonic acid in plasma using gas chromatography/mass spectrometry.
The combination of gas chromatography and mass spectrometry using a polar capillary column and isobutane chemical ionization made it possible to determine mevalonic acid (MVA) as the lactone at subnanogram levels using 1 mL of plasma. The pretreatment procedure consisted of only three steps, namely lactonization, washing with chloroform and liquid/liquid extraction. This simple, rapid and sensitive method, having good precision and accuracy, is useful for evaluating the change of plasma MVA, which is well correlated with whole-body cholesterol biosynthesis.
Department of Physical and Analytical Chemistry, Eisai Co. Ltd, Ibaraki, Japan.
Y
Y
Ishihama
N
N
Mano
Y
Y
Oda
H
H
Hiyoshi
H
H
Ikuta
Y
Y
Yoshida
D000328:Adult; D000818:Animals; D004285:Dogs; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007783:Lactones / Q000737:chemistry; D008297:Male; D008798:Mevalonic Acid / Q000097:blood*; D008875:Middle Aged
D007783:Lactones; D008798:Mevalonic Acid