Establishment of two distinct anti-cardiolipin antibody-producing cell lines from the same individual by Epstein-Barr virus transformation.
Two anti-cardiolipin antibody (ACA)-producing cell lines were established, using Epstein-Barr virus transformation followed by a repeated cluster-picking from the same individual with antiphospholipid syndrome who had a history of 8 consecutive fetal losses and deep venous thrombosis. Characterization of the two ACAs derived from these cell lines revealed that one (Ab-019, subclass IgM, kappa) reacted exclusively with cardiolipin and showed strong lupus anticoagulant activity, while the other (Ab-226, subclass IgM, lambda) reacted with negatively charged phospholipids such as phosphatidylserine and phosphatidylglycerol, as well as cardiolipin. Furthermore, Ab-226 showed reactivity with human umbilical vein endothelial cells whereas Ab-019 did not. It is suggested that ACA is heterogeneous even in the same individual, and that reactivity against negatively charged phospholipids corresponds to reactivity against endothelial cell.
Hasegawa
I
I
Department of Obstetrics and Gynecology, Niigata University School of Medicine, Japan.
Suzuki
T
T
Ishii
S
S
Takakuwa
K
K
Tanaka
K
K
D000328:Adult; D017153:Antibodies, Anticardiolipin / Q000096:biosynthesis*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000276:immunology*; D005260:Female; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans
D017153:Antibodies, Anticardiolipin