Mutability of Salmonella tester strains TA1538 (hisD3052) and TA1535 (hisG46) containing the UmuD' and UmuC proteins of Escherichia coli.
We have studied the mutability of Salmonella typhimurium tester strains carrying plasmids in which either the umuDC or the umuD'C operon of Escherichia coli have been cloned. Reversion of the hisD3052 frameshift mutation by benzo[a]pyrene (B[a]P), aflatoxin B1 (AFB1) and 1-nitropyrene (1-NP), was very efficiently promoted by UmuD' (the activated form of UmuD) and UmuC proteins. In contrast, UmuD'C proteins promoted a moderate reversion of the missense hisG46 allele by B[a]P, and were not effective in mediating this reversion by AFB1. The Salmonella tester strain carrying the hisD3052 allele and containing the E. coli UmuD'C proteins has a sensitivity toward frameshift mutagens similar to that of the MucAB containing strain TA98, and may be useful for obtaining a high level of mutants generated by the SOS mutagenic mechanism in the absence of MucAB proteins.
Instituto de Investigaciones Citológicas, Fundación Valenciana de Investigaciones Biomédicas, Spain.
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Urios
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Herrera
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Aleixandre
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Sommer
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Blanco
D016604:Aflatoxin B1 / Q000633:toxicity; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001564:Benzo(a)pyrene / Q000633:toxicity; D001711:Biotransformation; D004259:DNA-Directed DNA Polymerase; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D016368:Frameshift Mutation; D005798:Genes, Bacterial; D008862:Microsomes, Liver / Q000201:enzymology; D016254:Mutagenesis, Insertional; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D009876:Operon; D010957:Plasmids; D011721:Pyrenes / Q000633:toxicity; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D013045:Species Specificity; D013489:Suppression, Genetic
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D009153:Mutagens; D011721:Pyrenes; C068606:mucAB protein, E coli; D001564:Benzo(a)pyrene; C078202:UmuC protein, E coli; D016604:Aflatoxin B1; D004259:DNA-Directed DNA Polymerase; C055218:UmuD protein, E coli; C032668:1-nitropyrene