A cloned rat CD38-homologous protein and its expression in pancreatic islets.
Cyclic ADP-ribose recently has been suggested to be an important intracellular signal for insulin secretion. CD38, which was originally isolated from human lymphocytes as a surface marker, is active in the synthesis of cyclic ADP-ribose. We report here the cloning of a rat CD38-homologous protein (CD38H) which is expressed in pancreatic islets. The deduced amino acid sequence shows that rat CD38H is a protein of 303 amino acids (M(r), 34.4 kD) and contains one possible membrane-spanning domain, consistent with a type II transmembrane glycoprotein. The overall identity and similarity of the amino acid sequences between the human CD38 and the rat CD38H are 58% and 76%, respectively. RNA blot analysis showed a strong signal of 3.4 kb in rat brain, duodenum, and heart. CD38H also is shown to be expressed in pancreatic islets by the RT-PCR procedure, but its expression is not significantly different in Wistar and GK rats, a genetic model of non-insulin-dependent diabetes mellitus. The presence of rat CD38H in the pancreatic islets suggests that CD38H may be involved in insulin secretion by synthesizing cADP-ribose.
Li
Q
Q
Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Japan.
Yamada
Y
Y
Yasuda
K
K
Ihara
Y
Y
Okamoto
Y
Y
Kaisaki
P J
PJ
Watanabe
R
R
Ikeda
H
H
Tsuda
K
K
Seino
Y
Y
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D001048:Aplysia; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D036563:Cyclic ADP-Ribose; D017931:DNA Primers; D015870:Gene Expression; D006801:Humans; D007515:Islets of Langerhans / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D015703:Antigens, CD; D000943:Antigens, Differentiation; D017931:DNA Primers; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D036563:Cyclic ADP-Ribose; D000246:Adenosine Diphosphate Ribose; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; C498155:Cd38 protein, mouse; C498156:Cd38 protein, rat; D051997:ADP-ribosyl Cyclase 1