Characterization of two protein kinases from Schizosaccharomyces pombe involved in the regulation of DNA repair.
We have identified two novel genes designated hhp1+ and hhp2+ in the fission yeast Schizosaccharomyces pombe. The hhp1+ and hhp2+ genes encode two closely related protein kinases that share significant sequence identities with Hrr25p from Saccharomyces cerevisiae. Characterization of strains harboring single and double mutations in the hhp+ genes reveals DNA repair defects in these cells. Schizosaccharomyces pombe strains lacking either or both Hhp activities reveal differences in their ability to withstand DNA lesions caused by either methyl methanesulfonate (MMS) or gamma-rays which correlate with their ability to repair DNA strand breaks caused by these agents. We suggest that Hhp1 and Hhp2 are involved in the regulation of distinct and overlapping DNA repair pathways in S. pombe.
Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037.
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Dhillon
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Hoekstra
D000595:Amino Acid Sequence; D001483:Base Sequence; D047389:Casein Kinase I; D047388:Casein Kinases; D004260:DNA Repair; D004271:DNA, Fungal / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005720:Gamma Rays; D005800:Genes, Fungal / Q000235:genetics*; D005816:Genetic Complementation Test; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid
D004271:DNA, Fungal; D005656:Fungal Proteins; D007527:Isoenzymes; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; C088232:hhp1 protein, S pombe; C088231:hhp2 protein, S pombe; D047389:Casein Kinase I; D047388:Casein Kinases; C070775:HRR25 protein, S cerevisiae