Reduction in the contraction and intracellular calcium transient of single rat ventricular myocytes by gadolinium and the attenuation of these effects by extracellular NaH2PO4.
Gadolinium (Gd3+) is the most commonly used blocker of stretch-activated channels (SACs). The actions of Gd3+ in unstretched adult heart were investigated by observing its effects on the contraction and intracellular calcium transient of single rat ventricular myocytes. We found that Gd3+ caused a profound reduction in both these parameters at doses typically used to block SACs. However, the actions of Gd3+ were severely attenuated by the presence of 0.33 mM NaH2PO4, which possibly chelates the Gd3+ ion.
Department of Physiology, University of Leeds.
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Ward
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White
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000032:analysis* / Q000378:metabolism*; D004347:Drug Interactions; D005682:Gadolinium / Q000494:pharmacology*; D006352:Heart Ventricles / Q000737:chemistry; D008297:Male; D009200:Myocardial Contraction / Q000502:physiology*; D009206:Myocardium / Q000737:chemistry* / Q000166:cytology*; D010710:Phosphates / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010710:Phosphates; D005682:Gadolinium; C018279:sodium phosphate; D002118:Calcium