Bacteria invading periapical cementum.
The aim of this study was to determine whether microorganisms invade periapical cementum of human teeth from the adjacent periapical lesions. We therefore attempted to isolate microorganisms from periapical cementum through the adoption of standard anaerobic procedures for obligate anaerobes. Samples of cementum were taken from 10 amputated tooth roots at the time of apicoectomy. From two of these samples, bacteria were recovered after anaerobic incubation, but no bacteria were recovered after aerobic incubation of the same samples. Of a total of eight isolates from the cementum, seven were obligate anaerobes and one was aerotolerant. The obligate anaerobes isolated were assigned to the genera Prevotella, Peptostreptococcus, Eubacterium, and Fusobacterium. The aerotolerant anaerobe was Campylobacter. From this, we conclude that bacteria can successfully invade cementum via periapical periodontal tissue, and that such bacteria may play a significant role in chronic periapical pathosis.
Kiryu
T
T
Department of Operative Dentistry and Endodontics, Niigata University School of Dentistry, Japan.
Hoshino
E
E
Iwaku
M
M
D000190:Actinomyces / Q000302:isolation & purification; D000328:Adult; D000368:Aged; D001421:Bacteria, Anaerobic / Q000302:isolation & purification / Q000472:pathogenicity*; D001439:Bacteroides / Q000302:isolation & purification; D002167:Campylobacter / Q000302:isolation & purification; D002908:Chronic Disease; D003739:Dental Cementum / Q000382:microbiology*; D005051:Eubacterium / Q000302:isolation & purification; D006095:Gram-Positive Cocci / Q000302:isolation & purification; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification; D008875:Middle Aged; D010462:Peptostreptococcus / Q000302:isolation & purification; D010483:Periapical Diseases / Q000382:microbiology*; D011842:Radicular Cyst / Q000382:microbiology; D013291:Streptococcus / Q000302:isolation & purification