Changes in ovulatory and steroidogenic responses in mice after administration of the ribosome inactivating proteins momorcochin, luffaculin and luffins.
1. Immature mice were induced to ovulate by pregnant serum gonadotropin and human chorionic gonadotropin. 2. The ribosome inactivating proteins luffaculin, luffin-a, luffin-b and momorcochin were administered i.p. 3. It was found that all four ribosome inactivating proteins elicited a reduction in the circulating titer of estradiol-17 beta. 4. Both luffaculin and momorcochin brought about an increase in the number of degenerated oocytes. 5. Only momorcochin caused a decrease in the ovarian weight and the number of ovulated oocytes.
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Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T.
Chan
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Yeung
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HW
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000097:blood*; D005260:Female; D006023:Glycoproteins / Q000494:pharmacology; D006064:Gonadotropins, Equine / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction; D010940:Plant Proteins / Q000494:pharmacology*; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects
D006063:Chorionic Gonadotropin; D006023:Glycoproteins; D006064:Gonadotropins, Equine; D010940:Plant Proteins; D012269:Ribosomal Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C063258:luffin-a protein, Luffa cylindrica; C069038:luffin-b protein, Luffa cylindrica; D004958:Estradiol