The effect of granulocyte factor and grass pollen allergen on T-lymphocytes from atopic patients in vitro.
In allergy the immune response is significantly modified by inflammatory processes. Polymorphonuclear leukocytes (PMNLs) are involved in inflammatory processes. Activated PMNLs release many substances, including granulocyte factor (GF), which exerts immunomodulating effects. The present study was performed to determine the effects of allergens and/or GF on the expression of lymphocyte differentiation antigens in short-term cultures and to evaluate the production of migration inhibitory factor (MIF) under the influence of these substances. The studies were carried out on peripheral blood mononuclear cells isolated from patients with type I hypersensitivity, before and after the grass pollen season, and from healthy subjects. GF and allergens were found to increase the CD8 cell number, particularly in 7-day cultures and in patients before exposure to allergens, which correlated with MIF release in these patients under the influence of these factors. The results suggest that the PMNLs may participate in allergic inflammatory reactions.
Department of Pathophysiology & Immunology, Institute of Basic Medical Sciences, Military Medical Academy, Lódź, Poland.
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Kocur
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Zeman
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Tchorzewski
D000328:Adult; D000485:Allergens / Q000494:pharmacology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D002478:Cells, Cultured; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008263:Macrophage Migration-Inhibitory Factors / Q000378:metabolism; D008297:Male; D006109:Poaceae; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D000485:Allergens; D000945:Antigens, Differentiation, T-Lymphocyte; D008263:Macrophage Migration-Inhibitory Factors