Cloning and characterization of the pho-2+ gene encoding a repressible alkaline phosphatase in Neurospora crassa.
The Neurospora crassa phosphate-repressible alkaline phosphatase-encoding gene pho-2+ was cloned and its nucleotide sequence was determined. An open reading frame was found that contains four introns and encodes a putative protein of 555 amino acids. 'Activator-independent expression' of ectopically integrated pho-2+ was observed, as noted before for ectopically integrated pho-4+.
Grotelueschen
J
J
Biomolecular Chemistry Department, University of Wisconsin, Madison 53706.
Peleg
Y
Y
Glass
N L
NL
Metzenberg
R L
RL
D000469:Alkaline Phosphatase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics
D004274:DNA, Recombinant; D000469:Alkaline Phosphatase