Development of an RNA/RNA hybridization assay for the detection of the HAV CF53 strain.
A quick and sensitive dot-blot assay using non-radioactive labelled RNA probes was developed for the detection of the CF53 strain of hepatitis A virus (HAV) in cell culture. The cDNA of the 5' end of the HM175 strain was inserted in a transcription vector pSPT18 and was used to synthesize 32P- or digoxigenin-labelled RNA probes. These RNA probes specifically detected the RNA of the CF53 strain and can be used to detect HAV in PLC/PRF/5 cells. The sensitivity of non-radioactive tests was comparable to that of radiolabelled probes.
van Cuyck-Gandré
H
H
Unité de Biologie Moléculaire, Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Livache
T
T
Ringeaud
J
J
Crance
J M
JM
D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D006507:Hepatovirus / Q000302:isolation & purification*; D015151:Immunoblotting / Q000379:methods*; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012367:RNA, Viral / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D015347:RNA Probes; D012367:RNA, Viral