HPLC purification and separation of 5,5'-substituted-2,4-imidazolidinedithiones.
Several 5,5'-substituted-2,4-imidazolidinedithiones, synthesized from aldehydes or ketones, have been purified by HPLC using poly(styrene-divinylbenzene) packings. Purified 5,5'-substituted-2,4-imidazolidinedithiones have been identified in column effluent by UV absorbance and corroborated by mass spectrometry. Several silica-based, polymeric, and poly(styrene-divinylbenzene)-based packings were evaluated as matrices for resolution of a mixture of purified 5,5'-substituted-2,4-imidazolidinedithiones.
Hill
J C
JC
Division of Cytokine Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892.
Flannery
G M
GM
Fraser
B A
BA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D013867:Thiohydantoins / Q000302:isolation & purification*
D013867:Thiohydantoins