[Different manifestation of DNA binding protein in healthy individuals and cancer patients].
By a modified serum 64-DP isolation method we successfully isolated alpha-DNA binding protein (alpha DBP) to electrophoretic purity. Analysis by SDS-PAGE revealed a molecular weight of 59,000. It suggested that alpha DBP is a glycoprotein. Goat anti-alpha DBP anti-serum was prepared and single radial immunodiffusion assay was used to screen 256 healthy individuals (teachers, students, workers and peasants) and serum samples from 969 patients with various kinds of cancers. Contrary to previous findings, we found that serum alpha DBP was abundant in healthy individuals with homogeneous precipitation rings, and was not significantly increased in the serum of cancer patients. However, it depicted a heterogeneous pattern with 1-4 rings of various thickness. This phenomenon was observed in 94.2% of patients with liver cancer regardless of the presence or absence of AFP. We would suggest that the change of alpha DBP band from homogeneity to heterogeneity may be a sign of carcinogenesis in the body.
Lu
X F
XF
Institute of Infectious Diseases, Zhejiang Medical University, Hangzhou.
Hu
Z R
ZR
Zhao
Z L
ZL
D004268:DNA-Binding Proteins / Q000097:blood* / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion / Q000379:methods; D008113:Liver Neoplasms / Q000097:blood; D009369:Neoplasms / Q000097:blood*
D004268:DNA-Binding Proteins; D007106:Immune Sera