ELISA screening of monoclonal antibodies to haptens: influence of the chemical structure of hapten-protein conjugates.
An analysis has been made of the influence of the chemical structure of hapten-protein conjugates on ELISA based screening monoclonal antibodies to haptens. Evidence has been obtained that the size of haptens should be taken into account. For haptens with a relatively large molecular weight, one can use a conjugate with a carrier protein differing from the immunogen. For small haptens, the screening should be conducted with a conjugate differing from the immunogen with respect to both the carrier protein and the chemical linkage between the hapten and the protein. In addition, the hapten-protein coupling should involves different amino acid residues.
Laboratory of Hybridomas, Institute of Biotechnology, Moscow, Russia.
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Danilova
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000737:chemistry / Q000276:immunology*; D002417:Cattle; D004077:Digoxin / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D015394:Molecular Structure; D010634:Phenobarbital / Q000737:chemistry / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D013806:Theophylline / Q000737:chemistry / Q000276:immunology; D013974:Thyroxine / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D006241:Haptens; D012710:Serum Albumin, Bovine; D004077:Digoxin; D006433:Hemocyanins; D013806:Theophylline; C032808:keyhole-limpet hemocyanin; D013974:Thyroxine; D010634:Phenobarbital