Effect of concurrent exercise and physostigmine on lactate and pyruvate in plasma, muscle, and brain tissue of rats.
The purpose of this investigation was to determine the effect of physostigmine (Phy) and/or concurrent exercise on lactate, pyruvate, and L/P ratio in plasma, skeletal muscle, and brain tissue in male Sprague-Dawley rats. The Phy-dosed (Phy-D) and Phy-dosed + concurrent acute exercise (Phy-D + CAE) groups elicited significantly higher L/P ratios in plasma compared to the acutely exercised (AE) group at 30 min postexercise. Physostigmine dosing, with or without exercise, resulted in significantly lower muscle pyruvate levels, from 30 to 50 min postdrug administration, in Phy-D and Phy-D + CAE groups compared to the AE group. In the brain, lactate values were significantly elevated in the acutely exercised groups at 5 min postexercise with or without Phy dosing. However, at 15 to 30 min postexercise, lactate values were significantly elevated in the Phy-D + CAE compared to the AE group. These data suggest that when Phy is administered prior to a 20-min moderately intensive exercise bout, there is an accumulation of lactate for a prolonged period of time in recovery.
Department of Health and Physical Education, Syracuse University, NY 13244-5040.
P J
PJ
Buckenmeyer
S R
SR
Babu
R G
RG
Knowlton
S M
SM
Somani
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D007773:Lactates / Q000097:blood / Q000378:metabolism*; D019344:Lactic Acid; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D005082:Physical Exertion / Q000502:physiology*; D010830:Physostigmine / Q000494:pharmacology*; D011773:Pyruvates / Q000097:blood / Q000378:metabolism*; D019289:Pyruvic Acid; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D007773:Lactates; D011773:Pyruvates; D019344:Lactic Acid; D019289:Pyruvic Acid; D010830:Physostigmine