Adjuvant effect of liposomes and adamantylamide dipeptide on antigenicity of entrapped synthetic peptide derived from HIV-1 transmembrane region glycoprotein gp41.
The synthetic peptide antigen (Ag) (the primary structure Tyr-Leu-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly-Cys-Ser-Gly-Lys-Leu-Ile- Cys-Thr derived from the envelope glycoprotein gp41 of the human immunodeficiency virus type 1 (HIV-1) and exerting specificity with all HIV-1-positive sera available in the Czech Republic (and also in a panel of 10,000 sera from WHO)) was conjugated with bovine serum albumin (BSA) and encapsulated into liposomes. Adjuvant activities of liposomes with various lipid compositions were compared with Freund's complete adjuvant (FCA) and with aluminium hydroxide (AL). The immune response to BSA-Ag liposomes with coentrapped adamantylamide dipeptide (AdDP) was comparable with that of FCA in terms of longevity and levels of specific antibodies in mouse sera.
Veterinary Research Institute, Brno, Czech Republic.
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Turánek
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Toman
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J
Novák
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Krchnák
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P
Horavová
D000276:Adjuvants, Immunologic; D000547:Amantadine / Q000031:analogs & derivatives* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D004151:Dipeptides / Q000276:immunology*; D005260:Female; D015483:HIV Antibodies / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008081:Liposomes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D012710:Serum Albumin, Bovine; D014614:Vaccines, Synthetic / Q000276:immunology
D000276:Adjuvants, Immunologic; D004151:Dipeptides; D015483:HIV Antibodies; D015488:HIV Antigens; D015700:HIV Envelope Protein gp41; D008081:Liposomes; D010455:Peptides; D014614:Vaccines, Synthetic; D012710:Serum Albumin, Bovine; C044891:adamantylamide-alanyl-isoglutamine; D000547:Amantadine