Cryopreservation of microsurgically extracted ductal sperm: pentoxifylline enhancement of motility.
Microscopically extracted ductal sperm can be successfully cryopreserved using motility as the outcomes measure. The optimal regimen for cryopreservation of microscopically extracted ductal sperm awaits further determination. Pentoxifylline treatment improves initial post-thaw motility of cryopreserved microscopically extracted ductal sperm. The clinical efficacy of this practice awaits further determination. However, the best potential use for cryopreserved microscopically extracted ductal sperm may be IVF with subzonal or intracytoplasmic microinjection. If intracytoplasmic sperm injection becomes more widely available, then enhancement of motility with pentoxifylline may not be necessary. This approach allows for potential fertility insurance at the time of microsurgical bypass of obstruction, and it may preclude, the need for the andrologic surgeon to accurately time his sperm retrieval procedures with IVF. The latter potential advantage would significantly aid current logistical difficulties in the operating room.
Buch
J P
JP
Department of Surgery, University of Connecticut Health Center, Farmington 06030-3955.
Philips
K A
KA
Kolon
T F
TF
D015925:Cryopreservation; D004822:Epididymis; D005260:Female; D006801:Humans; D008297:Male; D008866:Microsurgery; D010431:Pentoxifylline / Q000494:pharmacology*; D013048:Specimen Handling; D013081:Sperm Motility / Q000187:drug effects*; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa; D014649:Vas Deferens
D010431:Pentoxifylline