Tracheoplasty without stent, using preshaped cryopreserved cartilage allografts in neonatal pigs.
The management of congenital and acquired laryngotracheal stenosis in children often includes tracheoplasty with different materials. Autologous cartilage is the most commonly described graft for tracheal reconstruction. In 12 1-week old piglets a tracheal defect was created and repaired using cryopreserved allografts sculpted before preservation into a newly designed shape. In half the animals (group 1), a free pericardial autograft was interposed on the luminal surface of the cartilage graft. A stent was not used in any animal. The animals grew normally and were killed after 2 months. The grafted tracheas showed maintenance of their structural support, and no evidence of narrowing of the grafted area in comparison to the trachea above the graft. The lumen was lined by ciliated respiratory epithelium. There was no identifiable pericardium at the time of sacrifice and no differences between the two groups. This study shows that in neonatal pigs, cartilage allografts, sculpted before cryopreservation, are a suitable alternative to autografts to cover tracheal defects and can be used without stenting. No advantages were identified when interposing a free pericardial graft.
Department of Pediatrics, University of Padua, Italy.
A
A
Messineo
R M
RM
Filler
T
T
Joseph
A
A
Bahoric
C R
CR
Smith
D000818:Animals; D000831:Animals, Newborn; D002356:Cartilage / Q000637:transplantation*; D015925:Cryopreservation; D008722:Methods; D011182:Postoperative Care; D013552:Swine; D014132:Trachea / Q000601:surgery*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous