Establishment of the ELISA for murine soluble gp130, a signal transducer for the IL-6 family cytokine, and its detection in the ascitic fluids of tumor-bearing mice.
Interleukin-6 (IL-6) signal is transduced through a membrane glycoprotein gp130, which associates with the IL-6 receptor alpha chain (IL-6R alpha) in the presence of IL-6. We prepared monoclonal antibodies (mAbs) specific to murine gp130 by immunizing rats and hamsters with a chimeric molecule consisting of the extracellular domain of murine gp130 and human immunoglobulin (Ig) G1 Fc (m130Ig). Furthermore, we developed a sandwich ELISA for detection of murine soluble gp130 (sgp130) and showed that sgp130 was present in the ascitic fluids of tumor-bearing mice. Because sgp130 can inhibit the biological activities of the IL-6-related cytokine subfamily that can enhance anti-tumor immune response and also has both growth inducing and growth inhibitory activities on various tumors, the results suggest that sgp130 in tumor-bearing host modulates tumor progression.
Division of Molecular Oncology, Osaka University Medical School, Japan.
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Matsuda
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Hirano
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001201:Ascites; D001402:B-Lymphocytes; D002460:Cell Line; D006224:Cricetinae; D050822:Cytokine Receptor gp130; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C494199:IL6ST protein, human; C494200:Il6st protein, mouse; C494201:Il6st protein, rat; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007377:Interleukin-3; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D050822:Cytokine Receptor gp130