Differential expression of multiple cell-surface heparan sulfate proteoglycans during embryonic tooth development.
Heparan sulfate accumulates on cell surfaces and at cell-matrix interfaces, and functionally modulates several of the effector molecules that support the interactions, growth, and differentiation of developing tissues. Using heparin sulfate-specific monoclonal antibodies MAb, we obtained evidence that extracts from rodent embryos contain multiple forms of cell surface-associated heparan sulfate proteoglycan (PG). Taking tooth development in the mouse embryo as a model to further investigate the relevance of this PG redundancy and using MAb against heparan sulfate, antibodies specific for syndecan (syndecan-1) and fibroglycan (syndecan-2) (two distinct members of a larger family of cell-surface heparan sulfate PGs), and specific cDNA probes for these two cell-surface PGs, we obtained in situ evidence for regulated and differential expression of multiple cell-surface heparan sulfate PGs. The unique, distinctive, and coordinated changes in the expressions of these PGs during morphogenesis and differentiation of dental tissues suggest that the various cell-surface PGs are not truly redundant but play important, specific, and potentially complementary roles during embryonic development.
Center for Human Genetics, University of Leuven, Belgium.
X M
XM
Bai
B
B
Van der Schueren
J J
JJ
Cassiman
H
H
Van den Berghe
G
G
David
D000818:Animals; D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006497:Heparan Sulfate / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008647:Mesocricetus / Q000196:embryology; D051379:Mice / Q000196:embryology; D009805:Odontogenesis / Q000502:physiology*; D011509:Proteoglycans / Q000378:metabolism*; D014070:Tooth / Q000196:embryology*
D000911:Antibodies, Monoclonal; D006497:Heparan Sulfate; D008562:Membrane Glycoproteins; D011509:Proteoglycans