Frequent occurrence of IgE and IgG4 antibodies against saliva of Aedes communis and Aedes aegypti mosquitoes in children.
We examined the prevalence of IgE and IgG4 class antibodies to the saliva of Aedes communis and Aedes aegypti mosquitoes in the sera of three groups of exposed children using a sensitive immunoblot method. The frequencies of IgE antibodies to the major 36-kD A. communis and A. aegypti saliva antigens ranged from 82 to 90% in the 20 Finnish, 17 Kenyan, and 20 Mexican children. The corresponding IgG4 antibody frequencies were 85, 41, and 20%, respectively. The nonexposed 20 Icelandic children did not show IgE or IgG4 antisaliva antibodies. Several of the Finnish children showed also IgE and IgG4 antibodies to a 22-kD A. communis saliva antigen. The Finnish children abnormally sensitive to mosquito bites had frequently IgE and IgG4 antibodies to the 22-kD A. communis saliva antigen, suggesting that these antibodies play a role in the pathogenesis of immediate cutaneous mosquito bite reactions. In contrast to this, no increase was found in the A. aegypti antibody frequencies in the Kenyan and Mexican children with papular urticaria, suggesting that humoral immune response to A. aegypti saliva is not involved in the development of this disorder. The present results show that humoral IgE and IgG4 immune responses to Aedes mosquito saliva antigens is common in children living both in temperature and tropical zones. The IgE antibodies seem to be involved in the immediate mosquito bite whealing, and the occurrence of the IgG4 subclass antisaliva antibodies might be an indicator of intense mosquito bite exposure.
Department of Dermatology, Helsinki University Hospital, Finland.
T
T
Reunala
H
H
Brummer-Korvenkontio
K
K
Palosuo
M
M
Miyanij
R
R
Ruiz-Maldonado
A
A
Löve
G
G
François
T
T
Palosuo
D000293:Adolescent; D000330:Aedes / Q000276:immunology*; D000818:Animals; D002648:Child; D002675:Child, Preschool; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification; D007223:Infant; D007630:Kenya; D008800:Mexico; D015995:Prevalence; D012463:Saliva / Q000276:immunology*; D012537:Scandinavian and Nordic Countries
D007074:Immunoglobulin G; D007073:Immunoglobulin E