Organization of the V gene segments in mouse T-cell antigen receptor alpha/delta locus.
The mouse T-cell receptor (TCR) alpha/delta locus was mapped using 17 V alpha and 4 V beta subfamily-specific probes. Four complementary methods were used: (1) an estimate of the V gene repertoire by Southern blot analysis of genomic DNA with subfamily-specific probes; (2) an analysis of V gene segments deleted by TCR gene rearrangements from a panel of T-cell tumors and hybridomas; (3) an analysis of overlapping clusters of cosmid clones; and (4) an analysis of large DNA fragments separated by field-inversion gel electrophoresis. The alpha/delta locus spans about 1 Mb. The distance between the 3'-most V gene segment (V delta 1) and the delta constant gene (C delta) is no more than 150 kb. Sixty-six V gene segments have been mapped physically on cosmids. The members of individual V alpha gene segment subfamilies are dispersed throughout the locus. In contrast, the V delta gene segments V delta 1 to 5 are clustered at the 3' end of the V gene segment cluster. At least two DNA segment duplications, 45 to 80 kb in length, are present in the locus. These data provide information on the evolution of the alpha/delta locus and on organization features that might influence the expression of specific V gene segments in gamma delta cells.
Division of Biology, California Institute of Technology, Pasadena 91125.
K
K
Wang
J L
JL
Klotz
G
G
Kiser
G
G
Bristol
E
E
Hays
E
E
Lai
E
E
Gese
M
M
Kronenberg
L
L
Hood
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D015342:DNA Probes; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D014644:Genetic Variation; D051379:Mice; D008806:Mice, Inbred AKR / Q000235:genetics*; D008807:Mice, Inbred BALB C / Q000235:genetics*; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D015183:Restriction Mapping; D017384:Sequence Deletion
D017931:DNA Primers; D015342:DNA Probes; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA