Interindividual variability of the human hepatic sulphotransferases.
The variability among subjects of the hepatic activities of O-sulphotransferase towards dopamine, p-nitrophenol, testosterone and ethinyloestradiol and of N-sulphotransferase with 1,2,3,4-tetrahydroisoquinoline (TIQ) as substrate is described. The rates of testosterone and TIQ sulphation were higher in men than women whereas those of ethinyloestradiol, dopamine and p-nitrophenol were similar in both sexes. The sulphotransferase activities towards p-nitrophenol and dopamine were positively skewed whereas those towards ethinyloestradiol approached normality. The coefficients of variations for the sulphotransferase activities ranged between 34% and 62% indicating a considerable variability among subjects. The rates of dopamine-, TIQ- and p-nitrophenol-sulphation were measured in the mucosa of the human intestine, and the duodenum/liver ratios were 10, 0.9 and 0.1, respectively. Thus the contribution of the intestine in the sulphation of xenobiotics is substrate dependent.
Pacifici
G M
GM
Department of Biomedicine, Medical School, University of Pisa, Italy.
Temellini
A
A
Castiglioni
M
M
D'Alessandro
C
C
Ducci
A
A
Giuliani
L
L
D000328:Adult; D000368:Aged; D015239:Arylsulfotransferase / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D007546:Isoquinolines / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D012727:Sex Characteristics; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000378:metabolism*; D044005:Tetrahydroisoquinolines
D007527:Isoenzymes; D007546:Isoquinolines; D044005:Tetrahydroisoquinolines; D004997:Ethinyl Estradiol; C014843:1,2,3,4-tetrahydroisoquinoline; D015238:Sulfotransferases; C084556:ethinylestradiol-17 alpha sulfotransferase; C087860:testosterone sulfotransferase; D015239:Arylsulfotransferase; D004298:Dopamine