[Variations of the biosynthesis and quantification of mRNA of human fibronectin during aging in culture].
There are during aging quantitative and qualitative alterations of the macromolecular components of extracellular matrix. The aim of our work was a comparative study of the biosynthesis of fibronectin, an adhesive molecule playing a role in the maintenance of tissue structure and function, with cultures of normal fibroblasts at different passages and the correlation with mRNA levels. There was an increased biosynthesis of fibronectin during fibroblast senescence as well as an increased mRNA coding for fibronectin. This suggests that fibronectin is a biomarker for replicative age.
Laboratoire de Biologie du Tissu Conjonctif, URA CNRS 1460, Faculté de Médecine, Créteil, France.
P
P
Rasoamanantena
J
J
Labat-Robert
S
S
Goldstein
D000328:Adult; D000375:Aging; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D012333:RNA, Messenger / Q000032:analysis*; D012867:Skin / Q000166:cytology
D005353:Fibronectins; D012333:RNA, Messenger
Variations de la biosynthèse et quantification de l'ARNm de la fibronectine humaine au cours du vieillissement en culture.