[Application of western blotting technique in the serodiagnosis of Mycobacterium tuberculosis infection].
We have used western blotting technique for examining antibodies in patients with tuberculosis and in healthy individuals. In patients with tuberculosis, the number and intensity of the developed antigen bands were much higher than in normal individuals. Furthermore, disease-associated protein antigens of 30,000-43,000, 28,000 and 25,000 were found to react with patients' sera. A specificity of 0.9639 and sensitivity of 0.9012 were obtained about 30,000-43,000 antigens. 25,000 antigen reacted with 41.74% of patients' sera but with none of the controls.
Basic Department of Shaanxi Provincial Geriatric Research Centre, Xian.
X D
XD
Lu
H P
HP
Sun
Y H
YH
Zhang
D000907:Antibodies, Bacterial / Q000032:analysis*; D015153:Blotting, Western; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012698:Serologic Tests; D014390:Tuberculosis, Meningeal / Q000175:diagnosis; D014396:Tuberculosis, Pleural / Q000175:diagnosis; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D000907:Antibodies, Bacterial