Cloning of Entamoeba genes encoding proteolipids of putative vacuolar proton-translocating ATPases.
Molecular cloning techniques were used to identify genes encoding the proteolipids of putative vacuolar proton-transporting ATPases (V-ATPases; EC 3.6.1.35) of Entamoeba histolytica (Ehvma3) and Entamoeba dispar. The Ehvma3 gene encoded a 177-amino-acid peptide, with an M(r) of 18,110, which showed extensive positional identities with peptides of E. dispar (92%), Schizosaccharomyces pombe (58%), and humans (56%).
Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts.
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Descoteaux
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Yu
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Samuelson
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004748:Entamoeba histolytica / Q000201:enzymology / Q000235:genetics*; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D010802:Phylogeny; D011510:Proteolipids / Q000235:genetics*; D006180:Proton-Translocating ATPases / Q000235:genetics*; D014617:Vacuoles / Q000201:enzymology*
D011510:Proteolipids; D006180:Proton-Translocating ATPases