High-level production of proteolytically sensitive secreted proteins in Escherichia coli strains impaired in the heat-shock response.
Department of Chemical Engineering, University of Texas at Austin 78712.
H J
HJ
Meerman
G
G
Georgiou
D001709:Biotechnology; D010450:Endopeptidases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics
D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D010450:Endopeptidases; D001618:beta-Lactamases