Proton uptake by cytochrome c oxidase on reduction and on ligand binding.
On reduction, cytochrome oxidase was found to take up 2.4 +/- 0.1 protons in the pH range 7.2-8.5, of which 2 are associated with the binuclear centre, and the remaining fractional proton with haem a/CuA. Ligation to oxidised cytochrome oxidase of the azide, formate, fluoride or cyanide anions is accompanied by uptake of one proton. In the case of the reduced enzyme, no protonation changes are observed on binding O2 (Hallén S. and Nilsson T. (1992) Biochemistry 31, 11853-11859) or CO. Cyanide binding to reduced oxidase is, in contrast, still accompanied by uptake of a proton. These findings are discussed in terms of our previously-published proposal for the ligand chemistry of the binuclear site. The results overall suggest a principle of electroneutrality of redox and ligand state changes of the binuclear centre, with charge compensations provided only by protonation reactions.
Glynn Research Institute, Bodmin, UK.
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R
Mitchell
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Rich
D000818:Animals; D000838:Anions; D001386:Azides / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism; D002417:Cattle; D003486:Cyanides / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005486:Flavin Mononucleotide / Q000378:metabolism; D005459:Fluorides / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000201:enzymology; D010084:Oxidation-Reduction; D011522:Protons
D000838:Anions; D001386:Azides; D003486:Cyanides; D005561:Formates; D011522:Protons; D005486:Flavin Mononucleotide; D002248:Carbon Monoxide; D003576:Electron Transport Complex IV; D005459:Fluorides