[Regulation of proliferation of stromal fibroblasts with methylprednisolone in cultures in vitro].
The effect of methylprednisolone [MP) in the concentration 10(-7) M on cloning capacity and proliferative potential of splenic and medullary stromal fibroblast precursors was studied in hematologically normal children. Medullary fibroblast culturing was performed according to A. Ya. Fridenstein method, splenic fibroblast culturing by an original procedure proposed by the authors. MP stimulated proliferation of human marrow clonogenic fibroblasts more profoundly than a xenogenic feeder could do. A successive introduction of MP and the feeder gives no rise to synergism, while their simultaneous application induces a blocking effect. Basing on these findings it is suggested that MP and the feeder compete for the same receptors on the adhesive cells of the explant. Splenic fibroblasts proved sensitive to neither xenogenic feeder nor to MP in concentration 10(-7) M.
Vladimirskaia
E B
EB
Aĭtmanbetova
A A
AA
Kryzhanovskiĭ
O I
OI
Osipova
E Iu
EIu
Zamaraeva
N V
NV
RumiantsevAG
D000293:Adolescent; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008775:Methylprednisolone / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects*
D008775:Methylprednisolone
Reguliatsiia proliferatsii stromal'nykh fibroblastov metilprednizolonom v kul'turakh in vitro.