Catalytic antibodies: perusing combinatorial libraries.
Combinatorial libraries are a promising alternative for isolating catalytic antibodies produced by the immune system in response to the transition-state analog of a given reaction. Large, diverse panels of antibodies with high affinity for the transition-state analog can be isolated using screening or selection approaches. Furthermore, we have estimated that nucleotide sequences that bear close similarity to the sequence for a known catalytic antibody occur in combination at frequencies sufficient for their detection in such libraries.
Department of Chemistry, Pennsylvania State University, University Park 16802.
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Posner
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Smiley
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Lee
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Benkovic
D000595:Amino Acid Sequence; D017151:Antibodies, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D011994:Recombinant Proteins
D017151:Antibodies, Catalytic; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins