LPS-inducible nuclear factor in human monocytes that binds the negative regulatory element of the HIV LTR.
We studied the constitutive and lipopolysaccharide (LPS)-induced expression of nuclear protein binding to the negative regulatory element (NRE) of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in fresh human monocytes. We demonstrated the existence of a constitutive factor binding to the NRE 73-bp HpaII/HpaII fragment (-216 to -143) whose expression is up-regulated by LPS treatment. Competition experiments with overlapping oligonucleotides covering the HpaII/HpaII fragment and with mutated oligonucleotides mapped the binding within the TTTCATCAC region (-171 to -163). This binding pattern is unique to human monocytes.
Biological Carcinogenesis and Development Program, PRI/DynCorp, Turin, Italy.
T
T
Musso
M C
MC
Bosco
D L
DL
Longo
I
I
Espinoza-Delgado
A
A
Sica
G W
GW
Cox
G L
GL
Gusella
G
G
Forni
L
L
Varesio
D001483:Base Sequence; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D006678:HIV / Q000235:genetics; D016325:HIV Long Terminal Repeat / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D004279:DNA, Viral; D008070:Lipopolysaccharides; D009687:Nuclear Proteins