Determination of binding constants of ligands to proteins by affinity capillary electrophoresis: compensation for electroosmotic flow.
This paper describes the estimation of binding constants (Kb) between carbonic anhydrase B (CAB, EC 4.2.1.1, from bovine erythrocytes) and charged benzenesulfonamides by affinity capillary electrophoresis (ACE) under conditions in which the migration time is affected by changes in electroosmotic flow and by nonspecific interactions accompanying changes in the concentration of ligand. Comparisons of values of migration times of the protein of interest, and of "noninteracting" marker proteins, with those of a neutral internal standard provide the basis for corrections for variable electroosmotic flow; these corrections make possible the estimation of Kb and its uncertainty even in the presence of substantial variations in electroosmotic flow.
Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138.
F A
FA
Gomez
L Z
LZ
Avila
Y H
YH
Chu
G M
GM
Whitesides
D000818:Animals; D001665:Binding Sites; D002257:Carbonic Anhydrase Inhibitors / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002417:Cattle; D004586:Electrophoresis / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology; D007700:Kinetics; D008024:Ligands; D009995:Osmosis; D009997:Osmotic Pressure; D011506:Proteins / Q000378:metabolism*; D013449:Sulfonamides / Q000378:metabolism*; D000096926:Benzenesulfonamides
D002257:Carbonic Anhydrase Inhibitors; D002256:Carbonic Anhydrases; D008024:Ligands; D011506:Proteins; D013449:Sulfonamides; D000096926:Benzenesulfonamides