Enhanced chemiluminescent assays for acetylcholine.
Acetylcholine and choline chemiluminescent assays have limitations when these compounds are detected in small areas of mammalian nervous tissue. Use of 7-dimethyl-aminonaphthalene-1,2-dicarbonic acid hydrazide (7-DMAN), instead of luminol, gives a threefold increase in emitted light in the chemiluminescent assay for acetylcholine based on the coupled choline oxidase-peroxidase reaction. Addition of light enhancers, such as para-iodophenol or D-luciferin, to luminol or 7-DMAN further increased the light emission. Under these conditions the detection limit for acetylcholine was 650 femtomoles. This enhanced chemiluminescent assay should be convenient for the detection of in vivo and in vitro acetylcholine release from mammalian neurons.
Unité de Neurocybernétique Cellulaire, UPR 418 CNRS, Marseille, France.
J P
JP
Ternaux
M C
MC
Chamoin
D000109:Acetylcholine / Q000032:analysis*; D000110:Acetylcholinesterase; D000818:Animals; D002794:Choline / Q000032:analysis*; D049454:Firefly Luciferin; D006834:Hydrazines; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol; D008322:Mammals; D009281:Naphthalenes; D009420:Nervous System / Q000737:chemistry; D011874:Radiometry / Q000379:methods; D012680:Sensitivity and Specificity
D006834:Hydrazines; D007202:Indicators and Reagents; D009281:Naphthalenes; C045681:7-dimethylaminonaphthalene-1,2-dicarbonic acid hydrazide; D008165:Luminol; D049454:Firefly Luciferin; D000110:Acetylcholinesterase; D002794:Choline; D000109:Acetylcholine