Electrogenic and phosphatidylinositol-4-monophosphate-stimulated Cl- transport by Cl- pump in the rat brain.
Rat brain Cl(-)-ATPase was solubilized and reconstituted in asolectin liposomes. Phosphatidylinositol-4-monophosphate increased Cl(-)-ATPase and ATP-dependent Cl- uptake activities in proteoliposomes. The ATP-dependent Cl- uptake was inhibited by a Cl(-)-ATPase inhibitor, ethacrynic acid, and increased at an inside-positive membrane potential or in the presence of a protonophore. These findings suggest that Cl(-)-ATPase is an electrogenic Cl- transporter, or a primary Cl- pump, probably regulated by phosphoinositide turnover in vivo.
Department of Pharmacology, Kansai Medical University, Osaka, Japan.
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D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D027321:Anion Transport Proteins; D001692:Biological Transport / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004976:Ethacrynic Acid / Q000494:pharmacology; D008081:Liposomes; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D010713:Phosphatidylcholines; D018129:Phosphatidylinositol Phosphates / Q000494:pharmacology*; D010743:Phospholipids; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility
D027321:Anion Transport Proteins; D008081:Liposomes; D010713:Phosphatidylcholines; D018129:Phosphatidylinositol Phosphates; D010743:Phospholipids; C037178:phosphatidylinositol 4-phosphate; C024990:asolectin; D000251:Adenosine Triphosphatases; C031652:anion-sensitive ATPases; D004976:Ethacrynic Acid