Effect of diallyl trisulfide on induction of UDS by mutagenic drugs in primary rat hepatocytes.
Most of anticancer drugs are mutagenic. A possible exception is diallyl trisulfide (DAT), a component of garlic. It is an antimutagenic anticancer chemical although it is mainly used as antibiotic. Its modifying effect on induction of UDS by mutagenic mitomycin C (MMC), cyclophosphamide (CP) and cis-diamine dichloroplatin (DDP) was investigated with the UDS assay in the primary cultures of Wistar rat hepatocytes (hpc) using the autoradiographic technique. Results showed that 1.0-4.0 nmol/ml of DAT did not induce UDS and that MMC, CP and DDP resulted in a significant induction of dose-dependent UDS. DAT enhanced induction of UDS by these drugs. A dose-effect relationship was observed between dose of DAT and enhancement of induction of UDS. However, the mechanism of the enhancement is not clear.
Deng
D J
DJ
Beijing Institute for Cancer Research, China.
Mueller
K
K
Kasper
P
P
Mueller
L
L
D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D002945:Cisplatin / Q000494:pharmacology; D003520:Cyclophosphamide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D008934:Mitogens / Q000494:pharmacology*; D016685:Mitomycin / Q000494:pharmacology; D009152:Mutagenicity Tests; D051381:Rats; D017208:Rats, Wistar; D013440:Sulfides / Q000494:pharmacology*
D000498:Allyl Compounds; D008934:Mitogens; D013440:Sulfides; C042577:diallyl trisulfide; D016685:Mitomycin; D003520:Cyclophosphamide; D004247:DNA; D002945:Cisplatin