Selenium-arsenic interaction in renal cells: role of lysosomes. Electron microprobe study.
Selenium can modify the toxicity of different elements. Selenium and the element form a complex that is precipitated in the tissues and inhibits the toxicity of the element. We studied the interaction of arsenic and selenium at the subcellular level in rat kidney cells using electronic probe microanalysis that permits detection of elements in the intracellular organelles. This showed that arsenic and selenium are concentrated and precipitated in the lysosomes of the renal cells in the form of insoluble selenide (As2Se). In the long term, the lysosomes and their precipitate are eliminated in the urine. These processes enable the lethal toxicity of arsenic to be inhibited. This intralysosomal concentration and precipitation can serve as a model for the mechanism of interaction of selenium with other elements and can be compared with other mechanisms of concentration and precipitation of elements in the lysosomes.
SC27 of INSERM, Laboratory of Biophysics, Faculty of Medicine, Creteil, France.
J P
JP
Berry
P
P
Galle
D000818:Animals; D001151:Arsenic / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D004347:Drug Interactions; D004577:Electron Probe Microanalysis; D007668:Kidney / Q000737:chemistry* / Q000166:cytology* / Q000648:ultrastructure; D008247:Lysosomes / Q000737:chemistry / Q000502:physiology*; D008297:Male; D015388:Organelles / Q000737:chemistry / Q000648:ultrastructure; D011188:Potassium / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D013455:Sulfur / Q000032:analysis; D013997:Time Factors
D013455:Sulfur; D012643:Selenium; D001151:Arsenic; D011188:Potassium