[Primers for the nef gene in the diagnosis of HIV infection using the polymerase chain reaction].
A new system of primers and internal probe identifying the nef gene of HIV-1 was selected and tested for the laboratory diagnosis of HIV infection by means of PCR. The proposed primers and probe by their characteristics approached the optimal ones for PCR diagnosis. The diagnostic value of the above system was confirmed by the PCR analysis of blood samples from 29 patients including subjects with a doubtful diagnosis of HIV infection. The results of the PCR analysis using primers nef1/nef2 correlated with the results obtained by employing the widely used primers for the gag gene SK38/SK39 and attested to the high specificity and diagnostic efficiency of the proposed system.
Radiuk
S N
SN
Matsevich
G R
GR
Tarantul
V Z
VZ
Andzhaparidze
O G
OG
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis / Q000235:genetics; D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D015754:Genes, nef / Q000235:genetics*; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000175:diagnosis* / Q000235:genetics; D018023:HIV Seronegativity / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015483:HIV Antibodies
Praĭmery k genu nef v diagnostike VICh-infektsii s pomoshch'iu polimeraznoĭ tsepnoĭ reaktsii.