[Development of enzyme-linked immunosorbent assay for biliary apolipoprotein A-I and a diurnal change of apolipoprotein A-I concentration in human bile].
Enzyme-linked immunosorbent assay for measuring biliary apolipoprotein A-I was established. Utilizing this assay, a diurnal change of apolipoprotein A-I concentration in hepatic bile obtained from percutaneous transhepatic drainage was investigated. A biliary apolipoprotein A-I concentration changed from time to time, and correlations between apolipoprotein A-I and total protein concentration, cholesterol concentration and lithogenic index, r = 0.873, 0.863 and 0.567 respectively, were observed. Moreover, biliary total protein concentration was closely related with lithogenic index (r = 0.671). These data suggest that hepatic lithogenic bile may induce apolipoprotein A-I secretion into hepatic bile.
Second Department of Internal Medicine, Asahikawa Medical College.
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H
Nishimura
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T
Hasegawa
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H
Saitoh
D016632:Apolipoprotein A-I / Q000032:analysis*; D001646:Bile / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis; D002940:Circadian Rhythm; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans
D016632:Apolipoprotein A-I; D002784:Cholesterol