Purification of 14-3-3 protein and analysis of isoforms in chicken brain.
14-3-3 proteins are apparently ubiquitous eukaryotic proteins that comprise a large number of isoforms. We have used specific antibodies raised against each mammalian isoform to probe for 14-3-3 isoforms in adult hen brains. The results suggest that there is a remarkable degree of similarity in primary structure (at least in the regions containing the epitopes). Reverse-phase HPLC of the purified avian 14-3-3 proteins indicates a high overall degree of similarity in sequence and levels of expression of each isoform that are remarkably similar to their mammalian counterparts.
Patel
Y
Y
Laboratory of Protein Structure, National Institute for Medical Research, Mill Hill, London, UK.
Martin
H
H
Howell
S
S
Jones
D
D
Robinson
K
K
Aitken
A
A
D048948:14-3-3 Proteins; D000818:Animals; D001923:Brain Chemistry; D002645:Chickens; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D014446:Tyrosine 3-Monooxygenase