Differential regulation of the 2',3'-cyclic nucleotide 3'-phosphodiesterase gene during oligodendrocyte development.
The two major isoforms of 2',3'-cyclic nucleotide phosphodiesterase (CNP), 48 and 46 kDa, have recently been shown to be produced from a single gene by alternative splicing. In addition, messenger RNA encoding the larger isoform is transcribed from a separate promoter, approximately 1 kb upstream from that encoding the smaller isoform. We have investigated the expression of these two CNP isoforms and have found that they are differentially expressed during the process of oligodendrocyte maturation. In oligodendrocyte precursors, only the mRNA encoding the larger protein is found. At the time of oligodendrocyte differentiation, however, both CNP mRNAs are induced. These patterns of CNP expression are likely due to stage-specific transcriptional regulation of the two CNP promoters during the process of oligodendrocyte differentiation.
Department of Neurology, Hospital of the University of Pennsylvania, Philadelphia 19104.
S S
SS
Scherer
P E
PE
Braun
J
J
Grinspan
E
E
Collarini
D Y
DY
Wang
J
J
Kamholz
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000096:biosynthesis*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology* / Q000254:growth & development; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis*; D009474:Neurons / Q000201:enzymology; D009836:Oligodendroglia / Q000187:drug effects / Q000201:enzymology*; D009900:Optic Nerve / Q000201:enzymology* / Q000254:growth & development; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic
D007527:Isoenzymes; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases