Formation of 8-hydroxy-2'-deoxyguanosine in epidermis of hairless mice exposed to near-UV.
Six-week-old male albino hairless mice (Hos: Hr-1) were exposed to a near-ultraviolet (UV) fluorescent sun lamp (33.5 kJ/m2/hr; wave length > 270 nm with a peak at 312.5 nm) to investigate the induction of oxidative DNA damage in epidermal cells. Significantly higher levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) were detected in a dose-dependent manner in epidermis of mice exposed to near-UV than in those of control animals. The ratio of 8-OHdG in near-UV-exposed/unexposed control was 2.08 +/- 0.19 after 168 kJ/m2 exposure, P < 0.01; 3.49 +/- 0.36 after 335 kJ/m2 exposure, P < 0.01 (means +/- SE). The levels of 8-OHdG decreased with time after near-UV exposure, suggesting the presence of removal and/or repair mechanisms. This is the first report that oxidative DNA base modification is induced in vivo in epidermal cells by near-UV exposure. Oxidative DNA base modification may be one of the causes of sunlight-induced skin carcinogenesis.
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
Y
Y
Hattori-Nakakuki
C
C
Nishigori
K
K
Okamoto
S
S
Imamura
H
H
Hiai
S
S
Toyokuni
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008815:Mice, Inbred Strains; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine