[Determination of nucleolar organizer activity in the differential diagnosis of transition-cell tumors of the bladder].
Assessment of cells during cytologic diagnosis is based on a complex of morphologic features of the nucleus and nucleolus whose increased polymorphism is characteristic of tumor cells. Introduction in clinical cytology of the nucleoli silvering method permitted assessment of the activity of nucleolar organizers in which ribosomal RNA transcription is carried out determining further growth, maturation, and volume of a cell. Silver ions precipitate at sites of active transcription forming granules which may be analyzed. The number of argentophilic granules in transitional-cell tumors increases as differentiation of the studied cellular elements deteriorates. The highest activity of the nucleolar organizer was observed in poorly-differentiated cells. Silvering method may be widely used in studies of nucleolar morphofunctional organization and of complex metabolic processes in the cells.
Abenova
M T
MT
Shibanova
A I
AI
D002295:Carcinoma, Transitional Cell / Q000648:ultrastructure*; D003937:Diagnosis, Differential; D006801:Humans; D009697:Nucleolus Organizer Region / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000648:ultrastructure*
Opredelenie aktivnosti iadryshkovykh organizatorov pri differentsial'noĭ diagnostike perekhodno-kletochnykh opukholeĭ mochevogo puzyria.