Measurement of the individual pKa values of acidic residues of hen and turkey lysozymes by two-dimensional 1H NMR.
The pH dependence of the two-dimensional 1H nuclear magnetic resonance spectra of hen and turkey egg-white lysozymes has been recorded over the pH range 1-7. By monitoring the chemical shifts of the resonances of the various protons of ionizable residues, individual pKa values for the acidic residues have been determined for both proteins. The pKa values are displaced, with the exception of those of the residues in the active site cleft, by an average of 1 unit to low pH compared to model compounds.
Bartik
K
K
Chimie Organique E.P., Université Libre de Bruxelles, Belgium.
Redfield
C
C
Dobson
C M
CM
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D055592:Biophysical Phenomena; D001703:Biophysics; D002645:Chickens; D005260:Female; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009113:Muramidase / Q000737:chemistry*; D010063:Ovum / Q000201:enzymology; D014422:Turkeys
D009113:Muramidase