The amino acid sequence previously attributed to a protein kinase or a TCP1-related molecular chaperone and co-purified with phytochrome is a beta-glucosidase.
A 60 kDa protein (P60) co-purified with phytochrome was identified as avenacosidase, a beta-glucosidase which is part of the defense system of Avena sativa. An antiserum raised against P60 was used to isolate a cDNA clone coding for the complete amino acid sequence of P60. The cDNA-derived amino acid sequence contained the partial sequences described before for a protein kinase [(1989) Planta 178, 199-206] and for a TCP1-related molecular chaperone [(1993) Nature 363, 644-647] co-purified with phytochrome. We conclude that these activities were related to minor contaminants and that only sequences of avenacosidase had been obtained.
Botanisches Institut, Universität München, Germany.
S
S
Gus-Mayer
H
H
Brunner
H A
HA
Schneider-Poetsch
F
F
Lottspeich
C
C
Eckerskorn
R
R
Grimm
W
W
Rüdiger
D000595:Amino Acid Sequence; D016477:Artifacts; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins; D002523:Edible Grain / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010834:Phytochrome / Q000302:isolation & purification*; D011494:Protein Kinases; D011506:Proteins; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000235:genetics* / Q000302:isolation & purification*
D011506:Proteins; D010834:Phytochrome; D011494:Protein Kinases; C087992:avenacosidase; D001617:beta-Glucosidase; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins