Moderate alcohol consumption and loss of cerebellar Purkinje cells.
OBJECTIVE
To examine the dose-response effect of alcohol consumption on the number of cerebellar Purkinje cells.


METHODS
A prospective necropsy study combined with detailed reports on use of alcohol from a relative or friend. The number of Purkinje cells was counted in the anterior midsagittal section of the cerebellar vermis, the area of which was measured by computer assisted morphometry.


METHODS
Department of forensic medicine, University of Helsinki.


METHODS
66 men, aged 35 to 69 years, subjected to medicolegal necropsy because of sudden or violent death. The average all year daily alcohol consumption over the year was 0 to 10 g in 17 men, 11 to 80 g in 24 men, and more than 80 g in 25 men.


METHODS
Number of Purkinje cells, alcohol consumption.


RESULTS
The numbers and density of Purkinje cells in the cross section of vermis showed a consistent but weak decrease with increasing daily alcohol intake but not with age. A wide variation in the cell counts was observed, especially in men drinking more than 80 g, suggesting differences in the susceptibility to effects of alcohol. Compared with men drinking 40 g or less, a long term moderate consumption of an average of 41 to 80 g daily was associated with a significant average loss of 242 (95% confidence interval 45 to 439) Purkinje cells (15.2%) from a mean of 1583 to 1341 cells. In those drinking 81 to 180 g the average loss was 535 (259 to 811) cells (33.4%) to a mean of 1048 cells. The density of cells in the cross section of vermis also fell significantly by 0.9 cell/mm (0.1 to 1.7) when the daily consumption exceeded 40 g and by 1.4 cell/mm (0.3 to 2.5) when the intake was 81 to 180 g. Only three cases (4.5%) in the series showed macroscopical cerebellar atrophy.


CONCLUSIONS
Long term intake of moderate doses of alcohol daily for 20-30 years may damage the cerebellum before the onset of macroscopical atrophy. Despite distinct individual differences an all year average daily alcohol intake of 41-80 g results in a risk of significant loss of Purkinje cells.
Department of Public Health, University of Tampere, Finland.
P J
PJ
Karhunen
T
T
Erkinjuntti
P
P
Laippala
D000328:Adult; D000368:Aged; D000375:Aging / Q000473:pathology; D000428:Alcohol Drinking / Q000009:adverse effects* / Q000473:pathology; D001284:Atrophy; D001344:Autopsy; D002452:Cell Count; D004305:Dose-Response Relationship, Drug; D005235:Fatty Liver, Alcoholic / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009929:Organ Size; D011446:Prospective Studies; D011689:Purkinje Cells / Q000187:drug effects / Q000473:pathology*