Specific allergen stimulates increased motility of peripheral blood lymphocytes and neutrophils from atopics; the effect of specific immunotherapy.
The chemotactic response of peripheral blood lymphocytes and neutrophils from pollinotics and healthy subjects in pollen allergen gradients, before and after specific immunotherapy was investigated using modified Boyden method. It was found that lymphocytes and neutrophils from allergic patients only responded in a chemotactic way in the gradient of the specific allergen. This effect could be demonstrated also in the period when the patients were asymptomatic. Preincubation of the cells with antibodies against human IgE results in abrogation of this effect only in lymphocytes. Specific immunotherapy results in the increased motility of lymphocytes and neutrophils in the gradient of specific allergen just after immunotherapy and significantly decreased motility of these cells during the next exposure to allergen (pollen season) as compared to the values observed in these subjects before immunotherapy. Thus the reactivity of these cells is the dynamic process largely dependent upon exposure to allergen.
Department of Internal Medicine and Allergology, Medical Academy, Wrocław, Poland.
T
T
Zak-Nejmark
J
J
Małolepszy
M
M
Jutel
M
M
Wrzyszcz
G
G
Nadobna
D000328:Adult; D000485:Allergens; D000595:Amino Acid Sequence; D002630:Chemotactic Factors / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood*; D007073:Immunoglobulin E / Q000097:blood; D007167:Immunotherapy; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology; D011058:Pollen; D012680:Sensitivity and Specificity; D013268:Stimulation, Chemical
D000485:Allergens; D002630:Chemotactic Factors; D007073:Immunoglobulin E; D009240:N-Formylmethionine Leucyl-Phenylalanine