Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G alpha q and not G alpha 11.
G-proteins of the q family have been implicated as mediators of bombesin receptors action. We cloned Xenopus G alpha q and G alpha 11 and specifically disrupted the synthesis of either protein with selective antisense oligonucleotides. G alpha q antisense inhibited responses mediated by neuromedin B receptor (NMB-R) by 74%, though not by gastrin-releasing peptide receptor (GRP-R). G alpha 11 antisense had little effect on either GRP-R- or NMB-R-mediated responses. This suggests that NMB-R couples to G alpha q, and that GRP-R and NMB-R show distinct G-protein coupling preferences in the Xenopus oocyte.
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel.
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Shapira
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Way
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Lipinsky
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Oron
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Battey
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009865:Oocytes; D018004:Receptors, Bombesin / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
D016376:Oligonucleotides, Antisense; D018004:Receptors, Bombesin; D019204:GTP-Binding Proteins