Horizontal polyacrylamide gel electrophoresis for the separation of DNA fragments.
A discontinuous borate/formate buffer system is presented for horizontal polyacrylamide gel electrophoresis of DNA fragments. The resolution potential of the system could be altered by changing the total monomer concentration (5-9%T), the concentration of the crosslinker piperazine diacrylamide (2-5%CPDA), as well as the concentration of formate in the gel (40-120 mM), the leading ion of the buffer system. The separation of DNA fragments would be improved by increasing the migration distance from 22 to 28 cm. This discontinuous polyacrylamide gel electrophoresis system proved highly reproducible.
Institute of Legal Medicine, Justus-Liebig University, Giessen, Germany.
H
H
Haas
B
B
Budowle
G
G
Weiler
D002021:Buffers; D003432:Cross-Linking Reagents; D004247:DNA / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005561:Formates
D002021:Buffers; D003432:Cross-Linking Reagents; D005561:Formates; C030544:formic acid; D004247:DNA