Photoinactivation of bacteriophage lambda by kojic acid and Fe(III): role of oxygen radical intermediates in the reaction.
Kojic acid, a bacterial metabolite, intensively used in food and pharmaceutical industry has been reported to induce strand breaks in double stranded DNA. This genotoxic action of kojic acid was tested directly by using bacteriophage inactivation. The inactivating activity is mediated through DNA strand scissions by oxygen free radical intermediates. This was tested by quenching of the inactivation reaction in presence of radical scavengers. These results indicate the usefulness of a simple system to test various risk factors in dietary components.
Bhat
R
R
Department of Biochemistry, F/O: Life Sciences, A.M.U. Aligarh (U.P), India.
Hadi
S M
SM
D001386:Azides / Q000494:pharmacology; D010582:Bacteriophage lambda / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects; D002374:Catalase / Q000494:pharmacology; D004249:DNA Damage; D004279:DNA, Viral / Q000187:drug effects; D003676:Deferoxamine / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D005609:Free Radicals; D005798:Genes, Bacterial; D005819:Genetic Markers; D007501:Iron / Q000633:toxicity*; D008027:Light; D008353:Mannitol / Q000494:pharmacology; D017319:Photosensitizing Agents / Q000633:toxicity*; D011753:Pyrones / Q000633:toxicity*; D019810:Sodium Azide; D014466:Ultraviolet Rays
D001386:Azides; D004279:DNA, Viral; D005609:Free Radicals; D005819:Genetic Markers; D017319:Photosensitizing Agents; D011753:Pyrones; D008353:Mannitol; C011890:kojic acid; D019810:Sodium Azide; D007501:Iron; D002374:Catalase; D003676:Deferoxamine