Eicosapentaenoic acid enhances myo-inositol uptake in cultured human aortic smooth muscle cells.
The effects of elevated glucose and Eicosapentaenoic acid (EPA, 20:5) on myoinositol uptake in human aortic smooth muscle cells (HASMC) were evaluated. Myo-inositol incorporation into HASMC was dependent on an active transport system via Na(+)-K+ ATPase activity based on the results with Na+ deprivation and Ouabain (5 mM). Although glucose (27.5, 55 mM) inhibited 2-[3H] myo-inositol uptake, the addition of EPA (3 x 10(-4) M) prevented glucose-mediated inhibition. In addition, EPA potentiated Na(+)-K+ ATPase activity of HASMC. Since EPA decrease glucose-mediated inhibition of myo-inositol uptake, this agent might ameliorate aortic smooth muscle cell function associated with diabetes.
Department of Internal Medicine, University of Tsukuba, Japan.
Y
Y
Okuda
M
M
Ezure
T
T
Sawada
M
M
Mizutani
K
K
Tsukahara
M
M
Soma
K
K
Yamashita
D000328:Adult; D001011:Aorta; D002478:Cells, Cultured; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007294:Inositol / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D007294:Inositol; D015118:Eicosapentaenoic Acid; D005947:Glucose