ELISA-type titertray assay of IgM anti-GM1 autoantibodies.
We report an ELISA-type titertray assay for autoantibodies against the ganglioside GM1. Trays were coated with ganglioside GM1 and reacted with patients' sera; bound IgM was detected with rabbit antibody to human IgM. High-titer serum from a patient was used as calibrator, another patient's serum as the positive control, and the GM1-specific cholera toxin as the control for GM1 coating. Regression curves of serum titers obtained from different patients were linear and parallel. Intra- and interassay CVs were 4.0-7.8% and 5.5-16%, respectively. We detected antibodies at a titer of 1:250 in normal subjects. Analytical specificity of the calibrator serum against GM1 was demonstrated by immune thin-layer chromatography. Anti-GM1 antibodies were increased in patients with chronic inflammatory demyelinating polyradiculoneuropathy (P < 0.002) or multiple sclerosis (P < 0.01). In Guillain-Barré syndrome, preliminary longitudinal studies showed a decrease in anti-GM1 titer that was related to clinical recovery.
Bech
E
E
Department of Clinical Chemistry, Aarhus University Hospital, Denmark.
Jakobsen
J
J
Orntoft
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TF
D001323:Autoantibodies / Q000097:blood*; D018288:Carcinoma, Small Cell / Q000276:immunology; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D000431:Ethanol; D005677:G(M1) Ganglioside / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008175:Lung Neoplasms / Q000276:immunology; D016472:Motor Neuron Disease / Q000276:immunology; D009103:Multiple Sclerosis / Q000276:immunology; D011129:Polyradiculoneuropathy / Q000276:immunology; D012680:Sensitivity and Specificity; D012997:Solvents
D001323:Autoantibodies; D007075:Immunoglobulin M; D012997:Solvents; D005677:G(M1) Ganglioside; D000431:Ethanol