Interactions of ginsenosides with ligand-bindings of GABA(A) and GABA(B) receptors.
1. Total saponin fraction decreased the affinity of specific [3H]muscimol binding without changes in Bmax. Ginsenoside Rb1 Rb2, Rc, Re, Rf and Rg1 inhibited the specific [3H]muscimol binding to the high-affinity site. 2. Total saponin fraction increased the affinity of specific [3H]flunitrazepam binding. Ginsenoside Re and Rf enhanced specific [3H]flunitrazepam binding. 3. Total saponin fraction decreased the affinity of specific [35S]TBPS binding without changes in Bmax. Ginsenosides did not affect specific or non-specific [35S]TBPS binding. 4. Total saponin fraction decreased the affinity of specific [3H]baclofen binding without changes in Bmax. Ginsenoside Rc inhibited specific [3H]baclofen binding.
Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson 39216-4045.
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D000818:Animals; D001418:Baclofen / Q000378:metabolism; D001643:Bridged Bicyclo Compounds / Q000378:metabolism; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002240:Carbohydrate Sequence; D005445:Flunitrazepam / Q000378:metabolism; D036145:Ginsenosides; D041743:Herb-Drug Interactions; D008024:Ligands; D008297:Male; D008969:Molecular Sequence Data; D009118:Muscimol / Q000378:metabolism; D005894:Panax; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D018080:Receptors, GABA-B / Q000187:drug effects / Q000378:metabolism*; D012503:Saponins / Q000494:pharmacology*; D013462:Sulfur Radioisotopes; D014316:Tritium
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D036145:Ginsenosides; D008024:Ligands; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D012503:Saponins; D013462:Sulfur Radioisotopes; D014316:Tritium; D009118:Muscimol; D005445:Flunitrazepam; C037476:tert-butylbicyclophosphorothionate; D001418:Baclofen