Action of alpha-momorcharin, a ribosome inactivating protein, on cultured tumor cell lines.
1. alpha-Momorcharin, a glycoprotein isolated from seeds of the bitter gourd, Momordica charantia inhibited incorporation of [3H]thymidine, [3H]leucine and [3H]uridine into P388 (mouse monocyte-macrophage), J774 (Balb/c macrophage), JAR (human placental choriocarcinoma) and sarcoma S180 cell lines. 2. The most potent inhibitory effect was exerted on P388 cells and the smallest effect on sarcoma cells. 3. alpha-Momorcharin also enhanced the tumoricidal effect of mouse macrophages on mouse mastocytomal (P815) cells.
Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T.
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Ng
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Liu
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Sze
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Yeung
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007930:Leucine / Q000378:metabolism; D007941:Leukemia P388 / Q000188:drug therapy / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D012515:Mast-Cell Sarcoma / Q000188:drug therapy / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D010940:Plant Proteins / Q000494:pharmacology*; D012269:Ribosomal Proteins; D054788:Ribosome Inactivating Proteins; D012510:Sarcoma 180 / Q000188:drug therapy / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014529:Uridine / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D010940:Plant Proteins; D012269:Ribosomal Proteins; D014316:Tritium; C039582:MMC protein, Momordica charantia; D054788:Ribosome Inactivating Proteins; D007930:Leucine; D013936:Thymidine; D014529:Uridine