Contact hemolysin production by strains of enteroaggregative Escherichia coli isolated from children with diarrhea.
Forty-five strains of enteroaggregative Escherichia coli were tested for hemolytic activity with different culture media and erythrocytes from different species. Thirty-seven strains showed proteinase K-sensitive contact hemolysin activity with sheep erythrocytes when cultured in Casamino Acids-yeast extract broth supplemented with 1 mM calcium chloride and when cultured in nutrient broth media. The production of contact hemolysin was dependent on temperature, pH, and culture conditions.
Department of Microbiology, Saga Medical School, Japan.
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Haque
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Ohki
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Kikuchi
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Kohashi
D000818:Animals; D001422:Bacterial Adhesion; D002648:Child; D003967:Diarrhea / Q000209:etiology / Q000382:microbiology*; D004912:Erythrocytes; D004926:Escherichia coli / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000209:etiology / Q000382:microbiology*; D006168:Guinea Pigs; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013696:Temperature
D006460:Hemolysin Proteins