Displacement of Enterococcus faecalis from hydrophobic and hydrophilic substrata by Lactobacillus and Streptococcus spp. as studied in a parallel plate flow chamber.
The displacement of Enterococcus faecalis 1131 from hydrophobic and hydrophilic substrata by isolates of Lactobacillus casei 36 and Streptococcus hyointestinalis KM1 was studied in a parallel plate flow chamber. The experiments were conducted with either 10 mM potassium phosphate buffer or human urine as the suspending fluid, and adhesion and displacement were measured by real-time in situ image analysis. The results showed that E. faecalis 1131 was displaced by lactobacilli (31%) and streptococci (74%) from fluorinated ethylene propylene in buffer and that displacement by lactobacilli was even more effective on a glass substratum in urine (54%). The passage of an air-liquid interface significantly impacted on adhesion, especially when the surface had been challenged with lactobacilli (up to 100% displacement) or streptococci (up to 94% displacement). These results showed that the parallel plate flow system with real-time in situ image analysis was effective for studying bacterial adhesion and that uropathogenic enterococci can be displaced by indigenous bacteria.
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
K
K
Millsap
G
G
Reid
H C
HC
van der Mei
H J
HJ
Busscher
D000328:Adult; D000898:Antibiosis / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology; D013293:Enterococcus faecalis / Q000502:physiology*; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D008297:Male; D013291:Streptococcus / Q000502:physiology*; D014556:Urine / Q000382:microbiology