Interaction between lead toxicity and some sulphonamides in rabbits: effect on certain blood constituents and serum enzymes.
Two main equal groups of clinically healthy, non pregnant rabbits were classified into 4 subgroups (5 rabbits each). The 1st and 2nd subgroups were treated with sulphaquinoxaline or sulphadiazine in a single oral dose of 100 mg/kg b. wt., while the 3rd and 4th subgroups received a repeated oral dose of 100 mg/kg b. wt., daily for 5 successive days, respectively. The second main group received lead acetate in a dose of 4.2 mg/kg b. wt. per day for 2 months, then was classified as in case of the 1st main group and administered the respective sulphonamides in their recommended doses. The experimental lead intoxication was found to decrease the free delta-aminolevulinic acid dehydratase (delta-ALA-D) activity in blood of lead intoxicated rabbits after 4 and 8 weeks. Also, the ratio of free and with glutathione reactivated delta-ALA-D was increased 2.9 and 2.2 after 4 and 8 weeks, respectively as compared with before lead administration (1.19), indicating toxicity. The sulphonamide/creatinine ratio was increased after administration of both sulphonamides but higher in lead intoxicated rabbits as compared with healthy ones. The AST/ALT ratio was decreased 4 and 8 weeks after lead exposure. The AST, ALT and AST/ALT ratio, alkaline phosphatase, urea and creatinine were not altered in healthy rabbits. Repeated oral administration of sulphadiazine caused a significant increase in serum AST, ALT, alkaline phosphatase and creatinine level in healthy and lead intoxicated rabbits. On the other hand, AST/ALT ratio in both healthy and lead intoxicated rabbits was found to decrease 1 h after the last dose as compared with before treatment.
Atef
M
M
Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Youssef
S A
SA
Ramadan
A
A
Afifi
N A
NA
Muity
A A
AA
D000818:Animals; D003404:Creatinine / Q000378:metabolism; D004347:Drug Interactions; D005260:Female; D007854:Lead / Q000633:toxicity*; D000623:Porphobilinogen Synthase / Q000097:blood; D011817:Rabbits / Q000097:blood*; D013411:Sulfadiazine / Q000493:pharmacokinetics / Q000633:toxicity*; D013428:Sulfaquinoxaline / Q000493:pharmacokinetics / Q000633:toxicity*
D013411:Sulfadiazine; D007854:Lead; D003404:Creatinine; D000623:Porphobilinogen Synthase; D013428:Sulfaquinoxaline