Anti-CD31 delays platelet adhesion/aggregation at sites of endothelial injury in mouse cerebral arterioles.
The arterioles on the surface of the mouse brain (pial arterioles) were observed by in vivo microscopy. A focus of minor endothelial damage was produced in a single pial arteriole in each mouse by briefly exposing the site to a helium neon laser after an intravenous injection of Evans blue. Mice were injected 10 minutes before injury with a monoclonal antibody (MAb) to mouse CD31, also known as platelet endothelial cell adhesion molecule. This treatment doubled (P < .01) the time required for the laser to produce a recognizable platelet aggregate. In additional experiments, an MAb to mouse CD61 and an MAb to mouse intercellular adhesion molecule 1 had no effect. The data support previous observations indicating that platelet adhesion/aggregation in this model is induced by endothelial injury without exposure of basal lamina. The data are consistent with the hypothesis that the endothelial injury exposes or activates a platelet endothelial cell adhesion molecule on the endothelium which is blocked by the MAb directed against CD31. This may be the first demonstration of an effect of an anti-platelet endothelial cell adhesion molecule on platelet endothelial cell adhesion molecule on platelet adhesion/aggregation in vivo.
Rosenblum
W I
WI
Department of Pathology (Neuropathology), Virginia Commonwealth University, Medical College of Virginia, Richmond 23298-0017.
Murata
S
S
Nelson
G H
GH
Werner
P K
PK
Ranken
R
R
Harmon
R C
RC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D001160:Arterioles / Q000187:drug effects; D001921:Brain / Q000098:blood supply / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000276:immunology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000293:injuries*; D051379:Mice; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D019408:Platelet Endothelial Cell Adhesion Molecule-1
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D015815:Cell Adhesion Molecules; D019408:Platelet Endothelial Cell Adhesion Molecule-1