The influence of magnesium-pyridoxal-5'-phosphate-glutamate in comparison with probucol, alpha-tocopherol and trolox on copper-induced oxidation of human low density lipoprotein in vitro.
Low density lipoprotein (LDL) in the presence of magnesium-pyridoxal-5'-phosphate-glutamate (MPPG), pyridoxal-5'-phosphate (PP), alpha-tocopherol, probucol or trolox is more resistant against copper-induced oxidation as control-LDL in vitro. The efficiency of the drugs is: probucol > MPPG > trolox > alpha-tocopherol > PP. LDL oxidation is determined by its increasing negative surface charge, fragmentation of apolipoprotein B-100 and changes of the fatty acid content of LDL. The protection of the drugs depends on their concentration and incubation time. Different experiments point to the fact that copper-induced oxidation of LDL in vitro starts with the binding of copper at the apolipoprotein B-100, resulting in an increasing negative surface charge and fragmentation of the apolipoprotein B-100. Afterwards a decrease of LDL-bound linoleic acid (18:2) is measurable.
Technische Universität München, Lehrstuhl für Phytophathologie, Weihenstephan, Germany.
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C
Kögl
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Schneider
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Elstner
D001161:Arteriosclerosis / Q000097:blood; D002839:Chromans / Q000494:pharmacology*; D003300:Copper / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D005973:Glutamine / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D011341:Probucol / Q000494:pharmacology*; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
D002839:Chromans; D005227:Fatty Acids; D008077:Lipoproteins, LDL; D005973:Glutamine; D014810:Vitamin E; D011732:Pyridoxal Phosphate; C047672:magnesium-pyridoxal-5'-phosphate glutamate; D003300:Copper; D011341:Probucol; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid