Enzyme immunoassay of motilin in human plasma.
A sensitive and specific double-antibody enzyme-linked immunoassay (EIA) for detecting a motilin-like immunoreactive substance (M-IS) in human plasma has been developed. In competitive reactions, the motilin antibody was incubated with a plasma sample (or motilin standard) and beta-D-galactosidase-linked synthetic motilin. Free and antibody-bound enzymes were separated using an anti-rabbit IgG-coated immunoplate. Enzyme activity on the plate was determined by fluorophotometric analysis. This immunoassay allows the detection of 20 to 200 fmol/ml (54 to 540 pg/ml) of motilin. The mean level of M-IS detected in human plasma was 53.9 +/- 25.6 pg/ml.
Takeyama
M
M
Department of Clinical Pharmacy, Oita Medical University, Japan.
Asakura
S
S
Kawano
A
A
Mori
K
K
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009037:Motilin / Q000097:blood* / Q000276:immunology; D010446:Peptide Fragments; D013552:Swine; D001616:beta-Galactosidase / Q000032:analysis
D015415:Biomarkers; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D009037:Motilin; D001616:beta-Galactosidase