Immunolocalization of platelet-derived growth factor, transforming growth factor-beta, and fibronectin in acute megakaryoblastic leukemia manifesting tumor formation.
Acute megakaryoblastic leukemia (AMKL) manifesting myelofibrosis and tumor formation in the liver with marked increase of reticulin is described. The megakaryoblastic nature of the leukemic cells of the bone marrow and the hepatic tumor nodule was established by positive immunohistochemical stains for CD41a and CD41b on frozen tissue sections. Immunolocalization of the platelet-derived growth factor (PDGF) protein and transforming growth factor (TGF)-beta protein also was demonstrated in the leukemic cells of the bone marrow and the hepatic tumor. Further, the deposition of fibronectin that has been known as the ligand of CD41a molecule and collagen types I and IV were recognized in the extracellular matrix of the bone marrow and the hepatic tumor. These results suggest that specific expression of growth factor proteins by the leukemic cells may selectively regulate the fibrosis of the bone marrow as well as the tumor formation of AMKL. The expression of adhesion molecules and growth factor proteins by the leukemic cells and the deposition of extracellular matrix are discussed in relation to the myelofibrosis as well as the tumor-forming nature of AMKL.
Department of Pathology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
M
M
Kitagawa
S
S
Yoshida
I
I
Ishige
J
J
Minami
T
T
Kuwata
T
T
Tanizawa
R
R
Kamiyama
D003094:Collagen / Q000032:analysis; D016222:Fibroblast Growth Factor 2 / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D006133:Growth Substances / Q000032:analysis*; D006801:Humans; D016130:Immunophenotyping; D007797:Laminin / Q000032:analysis; D007947:Leukemia, Megakaryoblastic, Acute / Q000473:pathology*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000032:analysis
D005353:Fibronectins; D006133:Growth Substances; D007797:Laminin; D008562:Membrane Glycoproteins; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D003094:Collagen