Determination of alkylphosphocholines by high-performance liquid chromatography with light-scattering mass detection.
A rapid and sensitive method for the determination of five different alkylphosphocholines including the antineoplastic phospholipid analogues hexadecylphosphocholine and octadecylphosphocholine is presented. The method is based on the separation of the lipids by high-performance liquid chromatography and quantitation by light-scattering mass detection. The lower limit of detection is approximately 50 pmol for each alkyphosphocholine tested. Quantitation is linear over the range 0.05-75 nmol. Hexane-isopropanol extracts of cultured cells can be applied to the column without further cleanup. The high resolution of separation and the sensitivity of detection render this method useful for pharmacokinetic investigations dealing with the uptake of alkylphosphocholines into different types of cells.
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany.
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D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D008027:Light; D010713:Phosphatidylcholines / Q000032:analysis* / Q000097:blood / Q000652:urine; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet
D010713:Phosphatidylcholines