Nucleotide sequence and expression analysis of the Acetobacter xylinum phosphoglucomutase gene.
The Acetobacter xylinum gene (celB) encoding phosphoglucomutase (EC 5.4.2.2) has previously been cloned by complementation of cellulose-negative mutants. In the present report the nucleotide sequence of a 2.0 kb DNA fragment containing celB is described. Expression analysis using the bacteriophage T7 RNA polymerase promoter phi 10 resulted in identification of a probable translational start codon of celB, and this conclusion was confirmed by N-terminal amino acid sequencing of the recombinant protein. From the nucleotide sequence data it was deduced that celB encodes a protein with a calculated molecular mass of 59.6 kDa. A protein of similar size was visualized after in vitro transcription and translation, using the cloned 2.0 kb fragment as template. The results of an amino acid sequence comparison and a biochemical analysis indicated that the CelB protein is structurally and functionally related to the previously characterized human and rabbit phosphoglucomutases.
Brautaset
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Unigen Center for Molecular Biology, University of Trondheim, Norway.
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Fjaervik
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Valla
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D000595:Amino Acid Sequence; D000688:Amylose / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D041822:Gluconacetobacter xylinus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010733:Phosphoglucomutase / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D011994:Recombinant Proteins; D000688:Amylose; D010733:Phosphoglucomutase