Perfusion systems for hybridoma cells based on sedimentation in chambers and Erlenmeyer flasks.
An inclined sedimentation chamber and a modified 250-ml Erlenmeyer flask have been used as separation devices for perfusion fermentations with hybridoma cells. The maximum cell density is increased 2-16-fold compared to batch fermentations when the separation units are used. When the sedimentation chamber is used, IgG is continuously produced and the daily production is increased by a factor 3.7 compared to batch fermentation.
Department of Biotechnology, Technical University of Denmark, Lyngby.
K M
KM
Lassen
C
C
Emborg
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002452:Cell Count; D005285:Fermentation; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D010477:Perfusion / Q000295:instrumentation / Q000379:methods
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G