Structure of the Acetobacter methanolicus MB 129 capsular polysaccharide, and of oligosaccharides resulting from degradation by bacteriophage Acm7.
The capsular polysaccharide of Acetobacter methanolicus MB 129 consists of D-Glc, D-Gal, L-Rha, and L-glyceric acid in the molar ratios 1:1:1:0.3. Periodate oxidation, methylation analysis, solvolysis with HF, and detailed 1H and 13C NMR analysis resulted in the structure of the repeating unit shown below. [formula: see text] Bacteriophage Acm7-associated end-alpha-L-rhamnopyranoside hydrolase depolymerizes the CPS even in the presence of the O-acyl group, to give the respective hexa-, nona-, and dodeca-saccharides.
Grimmecke
H D
HD
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Borstel, Germany.
Knirel
Y A
YA
Kiesel
B
B
Voges
M
M
Rietschel
E T
ET
D000091:Acetobacter / Q000737:chemistry* / Q000254:growth & development / Q000276:immunology; D001435:Bacteriophages / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D002241:Carbohydrates; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial