Shared control of maltose induction and catabolite repression of the MAL structural genes in Saccharomyces.
Maltose utilization in yeast requires the presence of any one of the five unlinked, homologous MAL loci. Transcription of the two structural genes MALT (permease) and MALS (maltase) is induced by maltose and catabolite-repressed by glucose. MAL6T and MAL6S share a common 5' intergenic sequence; deletion studies within this sequence revealed a bi-directionally functioning upstream activation sequence (UASM) consisting of four 11 bp homologous sites. Activation of these sites by the MALR protein results in the coordinate expression of MAL6T and MAL6S. The basal promoter activates MALS expression to a greater extent than MALT and is located in a region that overlaps UASM. Deletion of several subsites within the UASM has an asymmetric effect on MAL gene expression, having a greater affect on MALT than on MALS. Catabolite repression of MAL6T and MAL6S by glucose is controlled at several levels. Using disruption mutants, the positively acting MAL1R protein was also found to play a role in catabolite repression of MAL6T and MAL6S.
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.
B
B
Yao
P
P
Sollitti
X
X
Zhang
J
J
Marmur
D001426:Bacterial Proteins; D001483:Base Sequence; D004252:DNA Mutational Analysis; D005656:Fungal Proteins / Q000235:genetics; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005800:Genes, Fungal / Q000235:genetics*; D005947:Glucose / Q000494:pharmacology*; D008320:Maltose / Q000494:pharmacology*; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017384:Sequence Deletion; D000520:alpha-Glucosidases / Q000235:genetics
D001426:Bacterial Proteins; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D012097:Repressor Proteins; C084528:malR protein, bacteria; D008320:Maltose; C021534:maltose permease; D000520:alpha-Glucosidases; D005947:Glucose