Capillary gas chromatographic determination with nitrogen-phosphorus detection of the calcium antagonist nicardipine and its pyridine metabolite M-5 in plasma.
A capillary gas chromatographic method with nitrogen-phosphorus detection for the simultaneous determination of nicardipine and its pyridine metabolite M-5 was developed. The method involves extraction of the plasma with hexane-methylene chloride (1:1, v/v), followed by evaporation of the organic phase. The extract is injected into a fused-silica capillary column coated with cross-linked 5% phenyl-methylsilicone. A temperature gradient (85-285 degrees C) is applied and the two products and the internal standard can be separated within 22 min. The limit of detection is 0.5 ng/ml for both products. The method is suitable for pharmacokinetic studies in humans.
Heymans Institute of Pharmacology, University of Ghent Medical School, Belgium.
M T
MT
Rosseel
R A
RA
Lefebvre
D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D009529:Nicardipine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D009584:Nitrogen / Q000032:analysis*; D010758:Phosphorus / Q000032:analysis*
D010758:Phosphorus; C052820:2-(N-benzyl-N-methylamino)ethyl methyl 2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylate; D009529:Nicardipine; D009584:Nitrogen