Interferon-beta endogenously produced by intratumoral injection of cationic liposome-encapsulated gene: cytocidal effect on glioma transplanted into nude mouse brain.
The cytocidal effect of endogenously produced human interferon-beta was tested on glioma transplanted into the brain of nude mice by intracranial injection of a cell suspension of human glioma cell line U251-SP. When plasmid pSV2IFN-beta, bearing the human interferon-beta gene, was encapsulated into cationic multilamellar liposomes composed of N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroyl phosphatidylcholine, and dioleoyl phosphatidylethanolamine in a molar ratio of 1:2:2 and injected intratumorally, human interferon-beta was produced in the tumor. The tumor completely disappeared if the injection was done shortly after the transplantation. Even when the tumor did not disappear, survival of the tumor-bearing nude mice was markedly prolonged. In control experiments made with normal brain having no glioma, interferon-beta was not detected.
Institute of Applied Biochemistry, Yagi Memorial Park, Gifu, Japan.
K
K
Yagi
Y
Y
Hayashi
N
N
Ishida
M
M
Ohbayashi
N
N
Ohishi
M
M
Mizuno
J
J
Yoshida
D000818:Animals; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005910:Glioma / Q000473:pathology / Q000628:therapy*; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010957:Plasmids; D014407:Tumor Cells, Cultured
D016899:Interferon-beta