Regeneration of lesioned rat optic nerve fibers is improved after neutralization of myelin-associated neurite growth inhibitors.
Optic nerve axons do not regenerate after lesions in postnatal mammals, except if peripheral nerve transplants are offered as a substrate. In the present study, regeneration was assessed after intracranial freeze-crush lesions of the optic nerve, which completely interrupted all axons. In rats lesioned at the age of 8-9 days and surviving for additional 5-6 days, regenerating retinal fibers were seen to enter and partially cross the lesion site, reaching a maximum distance of 0.8 mm (mean +/- S.E.M. = 0.62 +/- 0.07 mm) in the presence of brain-derived neurotrophic factor (BDNF). Without BDNF, almost all the axons were lost due to axonal die-back. This regeneration distance could be significantly enhanced, up to 1.9 mm, by application of a monoclonal antibody (mAB-IN-1) directed against oligodendrocyte- and myelin-associated neurite growth inhibitory proteins. Similar results were obtained in rats lesioned at 16-18 days and surviving for 2 weeks: whereas fibroblast growth factor (FGF) stimulated sprouting did not exceed distances of 0.5 mm (mean = 0.38 +/- 0.07 mm), FGF and IN-1 antibody treated rats showed regenerations up to 2.3 mm (mean = 1.53 +/- 0.15 mm). The specificity of this effect was confirmed by lesions of myelin- and oligodendrocyte-free optic nerves: optic nerves were locally X-irradiated at birth, day 2, 4 and 6, a procedure which kills the dividing oligodendrocyte precursor cells. When these myelin-free nerves were lesioned at 3 weeks of age, regeneration distances between 2.5 and 3.2 mm were observed 3 weeks later.(ABSTRACT TRUNCATED AT 250 WORDS)
Brain Research Institute, University of Zurich, Switzerland.
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D
Weibel
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Cadelli
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Schwab
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001369:Axons / Q000502:physiology; D019208:Brain-Derived Neurotrophic Factor; D002452:Cell Count; D005615:Freezing; D006131:Growth Inhibitors / Q000037:antagonists & inhibitors*; D009409:Nerve Crush; D009413:Nerve Fibers, Myelinated / Q000502:physiology*; D009414:Nerve Growth Factors / Q000494:pharmacology; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects*; D009836:Oligodendroglia / Q000528:radiation effects; D009900:Optic Nerve / Q000502:physiology* / Q000648:ultrastructure; D020221:Optic Nerve Injuries; D051381:Rats; D011917:Rats, Inbred Lew
D000911:Antibodies, Monoclonal; D019208:Brain-Derived Neurotrophic Factor; D006131:Growth Inhibitors; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins