The complete sequence of a human astrovirus.
We have determined the complete genomic sequence of human astrovirus serotype 1 isolated in Newcastle upon Tyne. The genome is 6813 nucleotides long and contains three sequential open reading frames (ORFs). The two closest to the 5' end are linked by a ribosomal frameshifting motif and contain sequence motifs indicative of non-structural virus proteins: serine protease and RNA-dependent RNA polymerase. A nuclear addressing sequence is also located here. The 3' ORF encodes the virion structural polypeptides as a polyprotein precursor. This genomic organization resembles that of the plant virus family Luteoviridae.
Division of Virology, School of Pathological Sciences, University of Newcastle upon Tyne, U.K.
M M
MM
Willcocks
T D
TD
Brown
C R
CR
Madeley
M J
MJ
Carter
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D005814:Genes, Viral / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D001257:Mamastrovirus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000235:genetics; D006113:United Kingdom
D012324:RNA-Dependent RNA Polymerase; D012697:Serine Endopeptidases