Elucidation of structural abnormalities of the X chromosome using fluorescence in situ hybridisation with a Y chromosome painting probe.
Particular regions of the X and Y chromosomes share DNA sequence homology to the extent that cross hybridisation occurs. Thus, chromosome painting with a whole Y chromosome probe consistently results in fluorescence on specific regions of the X chromosome as well as the complete Y chromosome. This phenomenon has been exploited to elucidate the structure of unusual X chromosome rearrangements, without Y involvement, in two females.
South Western Regional Cytogenetics Centre, Southmead Hospital, Bristol, UK.
R T
RT
Howell
R
R
Millener
S
S
Thorne
J
J
O'Loughlin
J
J
Brassey
A
A
McDermott
D000328:Adult; D002675:Child, Preschool; D002872:Chromosome Deletion; D015342:DNA Probes; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008607:Intellectual Disability / Q000235:genetics; D012729:Sex Chromosome Aberrations / Q000235:genetics; D014960:X Chromosome / Q000648:ultrastructure*; D014998:Y Chromosome
D015342:DNA Probes