Cytostatin, a novel inhibitor of cell adhesion to components of extracellular matrix produced by Streptomyces sp. MJ654-NF4. I. Taxonomy, fermentation, isolation and biological activities.
Cytostatin has been identified as a novel inhibitor of cell adhesion to components of extracellular matrix (ECM) in cultured broth of Streptomyces sp. MJ654-NF4. Though cytostatin did not inhibit EL-4 cell adhesion to ECM components such as laminin and fibronectin; it inhibited the adhesion of B16 melanoma cells to laminin and collagen type IV but not to fibronectin. It exhibited antimetastatic activity on B16 melanoma cells in mice. The cytotoxicity of cytostatin are also reported.
Institute for Chemotherapy, M. C. R. F. Shizuoka, Japan.
M
M
Amemiya
M
M
Ueno
M
M
Osono
T
T
Masuda
N
N
Kinoshita
C
C
Nishida
M
M
Hamada
M
M
Ishizuka
T
T
Takeuchi
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005285:Fermentation; D007939:Leukemia L1210 / Q000188:drug therapy; D008546:Melanoma, Experimental / Q000188:drug therapy; D051379:Mice; D009362:Neoplasm Metastasis / Q000503:physiopathology / Q000517:prevention & control; D010755:Organophosphates / Q000302:isolation & purification / Q000494:pharmacology*; D011753:Pyrones / Q000302:isolation & purification / Q000494:pharmacology*; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification*; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D016326:Extracellular Matrix Proteins; D010755:Organophosphates; D011753:Pyrones; C088526:cytostatin