Measurement of iminodipeptides in the serum of patients with prolidase deficiency using liquid chromatography-mass spectrometry.
Iminodipeptides containing C-terminal proline or hydroxyproline were determined in sera from patients with prolidase deficiency, in their mother's serum, and in the sera of unrelated controls, using liquid chromatography-mass spectrometry with an atmospheric pressure ionization interface system. The separation was carried out on a reversed phase column using 1 g/l aqueous trifluoroacetic acid-methanol (75 + 25, by vol.). The quasi-molecular ions ([M + H])+ of various iminodipeptides containing C-terminal proline and hydroxyproline were observed in the sera of patients with prolidase deficiency, using selected ion monitoring. The quasi-molecular ions ([M + H])+ of iminodipeptides containing C-terminal proline were not observed in the sera of normal subjects or the patients' mother, but the latter did contain various iminodipeptides with C-terminal hydroxyproline. This method proved useful for the determination of iminodipeptides in the sera of patients with prolidase deficiency.
Department of Chemistry, Kochi Medical School, Japan.
K
K
Sugahara
Z
Z
Jianying
Y
Y
Yamamoto
K
K
Yasuda
H
H
Kodama
H
H
Kodama
D002853:Chromatography, Liquid; D004150:Dipeptidases / Q000172:deficiency*; D004151:Dipeptides / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D011392:Proline / Q000097:blood
D004151:Dipeptides; D011392:Proline; D004150:Dipeptidases; C022449:proline dipeptidase; D006909:Hydroxyproline