Ki-67 immunostaining and DNA flow cytometry as prognostic factors in epithelial ovarian cancers.
Samples from malignant epithelial ovarian tumors were examined for expression of Ki-67, DNA ploidy and S-phase fraction (SPF). In addition, normal ovary, benign and borderline tumors were immunostained with Ki-67 to examine its role in ovarian carcinogenesis and to estimate the prognostic significance. For both Ki-67, ploidy and SFP a significant correlation to survival in malignancy was observed. Furthermore, SPF above 10% showed significant correlation to decreased survival in both stage subgroups I-II and III-IV, while for aneuploidy a decrease in survival could be detected only in localized disease. Ki-67 expression was present in tumor cells in the main part of borderline and malignant tumors and even in 2 benign counterparts, which might indicate an active state in these commonly believed dormant neoplasms. In the malignant group the Ki-67 correlation to survival seemed to be independent of staining intensity, although the observed decay in survival seemed to be faster in the strongly stained group.
Henriksen
R
R
Department of Gynecology and Obstetrics, University Hospital, Uppsala, Sweden.
Strang
P
P
Backstrom
T
T
Wilander
E
E
Tribukait
B
B
Oberg
K
K
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins / Q000032:analysis*; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000401:mortality / Q000473:pathology*; D010053:Ovary / Q000166:cytology / Q000473:pathology; D011003:Ploidies; D011379:Prognosis; D012016:Reference Values; D016019:Survival Analysis; D015996:Survival Rate; D013997:Time Factors
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins