Two-dimensional gas chromatography for the enantiomeric separation of ephedrine and phenaminum and their metabolites.
Two-dimensional gas chromatography was used for enantiomeric separation of ephedrine, phenaminum and their metabolites in human urine. The main column was coated with a 2,6-di-O-pentyl-3-O-trifluoroacetylated-alpha-cyclodextrin and an SE-54 phase column was used as precolumn. The results showed that two-dimensional chromatography could greatly simplify the sample preparation, eliminate the interference of sample matrices, and improve the resolution of the enantiomers of interest.
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences.
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X
Wang
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Jia
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H
Wan
D000661:Amphetamine / Q000302:isolation & purification* / Q000652:urine; D002849:Chromatography, Gas; D003505:Cyclodextrins; D003913:Dextroamphetamine / Q000302:isolation & purification / Q000652:urine; D004809:Ephedrine / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D010665:Phenylpropanolamine / Q000302:isolation & purification / Q000652:urine; D013237:Stereoisomerism; D013607:Tablets; D047391:alpha-Cyclodextrins
D003505:Cyclodextrins; D013607:Tablets; D047391:alpha-Cyclodextrins; D010665:Phenylpropanolamine; D000661:Amphetamine; D004809:Ephedrine; D003913:Dextroamphetamine; C032613:alpha-cyclodextrin