Concomitant p53 gene mutation and increased radiosensitivity in rat lung embryo epithelial cells during neoplastic development.
A rat lung cell population had been treated with benzo(a)pyrene, and a set of different epithelial cell lines was derived from it. These cell lines carried either a wild-type or mutant p53 gene and represented grading states of neoplastic development. We demonstrate here that the cells lacking both wild-type p53 alleles display a significant decrease in survival after gamma-irradiation with doses of 2 to 12 Gy, compared with their counterparts carrying wild-type p53 alleles. This is the first reported model in which cells bearing a mutation of the p53 gene display enhanced sensitivity to ionizing radiation.
Biard
D S
DS
Département de Pathologie et Toxicologie Expérimentale, CEA-Saclay, Gif sur Yvette, France.
Martin
M
M
Rhun
Y L
YL
Duthu
A
A
Lefaix
J L
JL
May
E
E
May
P
P
D000818:Animals; D001564:Benzo(a)pyrene; D002460:Cell Line; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D017353:Gene Deletion; D016158:Genes, p53 / Q000187:drug effects / Q000235:genetics*; D008168:Lung / Q000473:pathology*; D017354:Point Mutation; D011836:Radiation Tolerance; D051381:Rats
D001564:Benzo(a)pyrene