Murine experimental autoimmune hepatitis: nonspecific inflammation due to adjuvant oil.
Experimental autoimmune hepatitis (EAH) has been induced in several strains of mouse by immunization with liver cytosol plus Freund's complete adjuvant. Our goal was to validate EAH in female C57Bl/6 mice and to compare the ability of immunization with liver cytosol and hepatocyte plasma membranes in Freund's complete (CFA) or incomplete (IFA) adjuvants to induce EAH. Control mice received Hank's balanced salt solution (HBSS), alone or an emulsion of HBSS in either CFA or IFA. The severity of hepatitis in coded liver sections was compared. EAH-like lesions were found not only in mice immunized with liver antigens in CFA or IFA but also in mice injected with either CFA or IFA. Mice injected with HBSS alone showed no hepatitis. These results indicate that immunization with neither liver nor mycobacteria cell wall antigens is required for induction of EAH-like lesions and suggest that the EAH in C57Bl/6 mice may be a nonspecific inflammatory response to adjuvant oil.
Howell
C D
CD
University of Colorado, School of Medicine, Denver 80262.
Yoder
T D
TD
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology / Q000473:pathology*; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology / Q000633:toxicity*; D006520:Hepatitis, Animal / Q000276:immunology / Q000473:pathology*; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008099:Liver / Q000276:immunology / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009821:Oils; D010232:Paraffin
D009821:Oils; D010232:Paraffin; C015418:paraffin oils; D005620:Freund's Adjuvant