Effect of growth temperature on complement-mediated killing of mesophilic Aeromonas spp. serotype O:34.
Mesophilic Aeromonas spp. strains (serotype O:34) showed sensitivity to complement-mediated killing when they were cultivated at 37 degrees C (serum-sensitive) but not when they were cultivated at 20 degrees C (serum-resistant). These strains produced smooth lipopolysaccharide when they were grown at 20 degrees C and rough lipolysaccharide when cultivated at 37 degrees C. The reason for the resistance to complement-mediated killing could be that C3b is rapidly degraded (possibly because it is bound far from the cell membrane), consequently the lytic complex (C5b-9) is not formed.
Merino
S
S
Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Spain.
Alvarez
D
D
Hernández-Allés
S
S
Tomás
J M
JM
D000333:Aeromonas / Q000254:growth & development* / Q000276:immunology*; D001770:Blood Bactericidal Activity; D003179:Complement C3b / Q000276:immunology / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000276:immunology / Q000378:metabolism; D003165:Complement System Proteins / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism*; D013696:Temperature
D015938:Complement Membrane Attack Complex; D008070:Lipopolysaccharides; D003179:Complement C3b; D003165:Complement System Proteins