Parallel changes in Glut 4 and Rab4 movements in two insulin-resistant states.
Insulin-induced Glut 4 and Rab4 movements were studied in two insulin-resistant states. In adipocytes from streptozotocin diabetic rats, the amount of Glut 4 was decreased by 60%. The remaining Glut 4 molecules were translocated in response to insulin, and in parallel, Rab4 left the intracellular compartment. In contrast, in 3T3-L1 adipocytes rendered insulin-resistant by a prolonged insulin treatment, both Rab4 and Glut 4 remained in the intracellular compartment following an acute insulin stimulation. Those results illustrate a similar behavior of Glut 4 and Rab4 in two situations where insulin resistance results from different mechanisms, and add further support for a role of Rab4 in Glut 4 translocation.
Institut National de la Santé et de la Recherche Médicale, INSERM U 145, Faculté de Médecine, Nice, France.
J M
JM
Ricort
J F
JF
Tanti
M
M
Cormont
E
E
Van Obberghen
Y
Y
Le Marchand-Brustel
D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002451:Cell Compartmentation; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004822:Epididymis / Q000166:cytology; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D051275:Glucose Transporter Type 4; D007328:Insulin / Q000494:pharmacology; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D009124:Muscle Proteins; D051381:Rats; D013311:Streptozocin; D020695:rab4 GTP-Binding Proteins
D051275:Glucose Transporter Type 4; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495694:Slc2a4 protein, rat; D013311:Streptozocin; D019204:GTP-Binding Proteins; D020695:rab4 GTP-Binding Proteins; D005947:Glucose