Single-donor platelet concentrates stored in synthetic medium. In vitro and in vivo studies.
Single-donor platelet concentrates (PC) were prepared in 80-120 ml plasma and stored in two polyolefin bags after addition of 250 ml plasmalyte, a simple, glucose-free synthetic medium that was previously used for platelet storage; when compared to PC stored in plasma, PC stored in plasmalyte, showed similar platelet quality, morphology and function after 5 days of storage. In vivo increments observed after transfusion of PC stored for 5 days in plasmalyte were similar to those observed after transfusion of 1-2 day old PC stored in plasma. Moreover, transfusion of 5-day old PC stored in plasmalyte was associated with correction of prolonged bleeding times in all 3 of the 3 patients evaluated. It is concluded that plasmalyte seems to be promising as a medium for single-donor PC storage.
Division of Hematology, Maggiore Hospital, Milan, Italy.
F
F
Bertolini
F
F
Marangoni
A
A
Loy
M
M
Marconi
D
D
Almini
P
P
Rebulla
G
G
Sirchia
D001782:Blood Donors; D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology; D001793:Blood Preservation; D006801:Humans; D066298:In Vitro Techniques; D010949:Plasma; D010976:Platelet Count; D017713:Platelet Transfusion; D010983:Plateletpheresis; D012996:Solutions
D012996:Solutions