Sulfotransferase gene expression in rat hepatic and extrahepatic tissues.
Enzymatic sulfation has been implicated to play a key role in a number of essential biological pathways including xenobiotic detoxication, carcinogen activation, and the regulation of intra-tissue hormone activity. In order to increase our understanding of the critical determinants governing the regulation of sulfotransferase gene expression, we investigated age-, gender-, and xenobiotic-related alterations in hydroxysteroid sulfotransferase-a or aryl sulfotransferase-IV gene expression. Northern blot and slot blot analyses showed that rat hepatic hydroxysteroid sulfotransferase-a mRNA expression was responsive to age- and gender-related signals. The results also suggested that the rat hepatic aryl sulfotransferase-IV and hydroxysteroid sulfotransferase-a genes are differentially regulated. Northern blot and reverse transcriptase polymerase chain reaction analyses demonstrated that hydroxysteroid sulfotransferase-a mRNA was expressed to a greater extent in female rat liver than in lung or kidney tissue. In addition, rat hepatic hydroxysteroid sulfotransferase-a gene expression in mature female rats, although not substantially altered in response to short-term fasting or high-dose dexamethasone treatment, was suppressed after treatment with the polycyclic aromatic hydrocarbon, 3-methylcholanthrene.
Runge-Morris
M A
MA
Institute of Chemical Toxicology, Wayne State University, Detroit, MI 48201.
D000375:Aging / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D018076:DNA, Complementary / Q000737:chemistry; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012737:Sex Factors; D015238:Sulfotransferases / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D012333:RNA, Messenger; D015238:Sulfotransferases; C028152:alcohol sulfotransferase; C077557:tyrosine-ester sulfotransferase