Effect of mood stabilizing agents on agonist-induced calcium mobilization in human platelets.
The effect of mood stabilizing agents such as lithium, carbamazepine, valproic acid and clonazepam on serotonin(5-HT)- or thrombin-induced intracellular calcium (Ca) mobilization was studied in the platelets of healthy subjects using the fluorescent Ca indicator fura-2. After incubating platelet-rich plasma with these drugs for one or four hours, there was no significant difference in either basal Ca2+ concentration or 5-HT-stimulated Ca response between each agent treatment and control. 5-HT- or thrombin-induced Ca mobilization was not altered by four weeks of lithium carbonate administration in healthy volunteers. These results indicate that these mood stabilizers fail to affect the agonist-stimulated intracellular Ca mobilizing pathway either in vitro or ex vivo in the platelets of healthy subjects.
Department of Psychiatry and Neurology, Hokkaido University School of Medicine, Sapporo, Japan.
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Kusumi
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Koyama
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Yamashita
D000328:Adult; D000339:Affect / Q000187:drug effects*; D001792:Blood Platelets / Q000187:drug effects*; D002118:Calcium / Q000097:blood*; D015220:Calcium Channels / Q000187:drug effects*; D002220:Carbamazepine / Q000494:pharmacology*; D002478:Cells, Cultured; D002998:Clonazepam / Q000494:pharmacology*; D006801:Humans; D016651:Lithium Carbonate / Q000494:pharmacology*; D008297:Male; D012016:Reference Values; D014635:Valproic Acid / Q000494:pharmacology*
D015220:Calcium Channels; D016651:Lithium Carbonate; D002220:Carbamazepine; D002998:Clonazepam; D014635:Valproic Acid; D002118:Calcium