Maturation of human megakaryocytic cell line (CMK-7) on the human umbilical vein endothelial cell (EC) monolayer.
Human umbilical vein endothelial cells (EC) were studied to determine whether they were capable of stimulating the maturation of a human megakaryocytic cell line CMK-7. CMK-7 overlaid on EC monolayers in direct contact conditions (CMK-7EC1) and in non-contact conditions (CMK-7EC2), were compared with unstimulated CMK-7 (CMK-7US) and CMK-7 treated with 10(-8) M phorbol ester (CMK-7TPA). The increases in cell-size, CD41b and CD42 positivity, adenosine triphosphate (ATP) and serotonin (5-HT), platelet factor 4 (PF4), beta-thromboglobulin (beta-TG), and DNA contents, indicated both stimulated endomitosis and cytoplasmic maturation in the CMK-7TPA. In contrast, CMK-7EC1 increased in size, in ATP and 5-HT contents and in PF4 and beta-TG contents, without an increase in DNA content, indicating that only cytoplasmic maturation occurred. These findings showed that EC was able to stimulate the cytoplasmic maturation of CMK-7 only under direct contact conditions. Furthermore, the CMK-7EC1 on the EC monolayer developed long cytoplasmic processes that resembled those of megakaryocytes in the terminal stage.
Hoshi
M
M
Division of Hematology, University of Tsukuba, Japan.
Nagasawa
T
T
Abe
T
T
Satoh
T
T
Mitsui
Y
Y
D002460:Cell Line; D048429:Cell Size; D003593:Cytoplasm; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology*; D014471:Umbilical Veins