Regulation of xylanolytic enzymes in Bacillus subtilis.
The synthesis of the xylanolytic enzymes beta-xylanase and beta-xylosidase of Bacillus subtilis was studied. In contrast to many catabolic extracellular enzymes, beta-xylanase was synthesized constitutively during exponential growth and was not repressed by glucose. beta-Xylosidase synthesis was induced 100-fold by xylose and repressed 100-fold by glucose. Carbon catabolite repression was abolished in a ccpA mutant. Titration experiments using a multicopy operator sequence responsible for carbon catabolite repression indicated that the gene encoding beta-xylosidase is part of the same carbon catabolite repression regulon as the amyE and bgIS genes.
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität Greifswald, Germany.
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Lindner
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Stülke
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Hecker
D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism; D014994:Xylose / Q000378:metabolism; D014995:Xylosidases / Q000096:biosynthesis* / Q000235:genetics
D014990:Xylans; D014994:Xylose; D006026:Glycoside Hydrolases; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; C026579:exo-1,4-beta-D-xylosidase; D005947:Glucose