Effect of basic fibroblast growth factor and alpha-melanocytic stimulating hormone on nerve regeneration through a collagen channel.
An experimental study on the rat sciatic nerve was performed to evaluate nerve regeneration through a collagen guide and to study the effects of alpha-melanocytic stimulating hormone (alpha-MSH) and basic fibroblast growth factor (b-FGF) in accelerating axonal elongation. After transection, nerves were repaired over a 7 mm gap using a placental collagen type IV guide. The channel was filled with either a b-FGF solution or an alpha-MSH solution or was produced with b-FGF incorporated into the guide. Four weeks later, only groups in which b-FGF had been injected or incorporated displayed a significant somatosensory evoked potential response. Histological and quantitative analysis of nerve fibres confirmed the existence of nerve continuity in groups receiving an alpha-MSH solution or a channel containing b-FGF. These results demonstrate that alpha-MSH in solution and b-FGF incorporated into a collagen type IV channel enhance peripheral nerve regeneration. However, at 4 weeks, only b-FGF (3 ng) restores functional activity.
Laquerriere
A
A
Pathology Laboratory, Charles Nicolle Hospital, Rouen, France.
Peulve
P
P
Jin
O
O
Tiollier
J
J
Tardy
M
M
Vaudry
H
H
Hemet
J
J
Tadie
M
M
D000818:Animals; D001672:Biocompatible Materials; D003094:Collagen; D005073:Evoked Potentials, Somatosensory; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000521:alpha-MSH / Q000494:pharmacology*
D001672:Biocompatible Materials; D016222:Fibroblast Growth Factor 2; D000521:alpha-MSH; D003094:Collagen