Cloning and characterisation of the gene encoding cytochrome c4 from Pseudomonas stutzeri.
By use of a degenerate oligodeoxyribonucleotide probe corresponding to the N-terminal amino acid (aa) sequence and Southern blot analysis, the gene encoding the di-heme cytochrome c4 (CC4) from Pseudomonas stutzeri has been cloned. The aa sequence deduced from the nucleotide sequence shows a 20-aa signal peptide and a 190-aa mature protein with 79% identity to the Azotobacter vinelandii CC4 sequence, which had earlier been determined by aa sequencing.
Institute of Molecular and Cell Biology, National University of Singapore.
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Christensen
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000737:chemistry / Q000235:genetics*; D004274:DNA, Recombinant; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics
D003574:Cytochrome c Group; D004274:DNA, Recombinant; C034186:cytochrome C4