One-minute exposure of 4-cell mouse embryos to glucose overcomes morula block in CZB medium.
One-cell mouse embryos that block at the 2-cell stage can progress to the morula stage in CZB medium, but fail to cavitate and then swell and lyse. A 1-min exposure to 27 mM glucose at the 4-cell stage (approximately 42 hr) will support a high frequency of development to the blastocyst stage (75%) in the same medium. A glucose exposure is beneficial anytime between 30 and 54 hr of culture (67-73% blastocysts). Of a group of additional sugars and glucose analogues tested for their ability to replace glucose, only galactose was equivalent in promoting embryo development to the blastocyst stage (64% blastocysts).
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts.
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Chatot
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J
Lewis-Williams
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I
Torres
C A
CA
Ziomek
D000818:Animals; D002970:Cleavage Stage, Ovum / Q000187:drug effects* / Q000502:physiology; D003470:Culture Media / Q000494:pharmacology; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D051379:Mice; D009024:Morphogenesis / Q000187:drug effects; D009028:Morula; D013997:Time Factors
D003470:Culture Media; D005947:Glucose; D005690:Galactose