Interaction of higher plant ribosomal 5S RNAs with Xenopus laevis transcriptional factor IIIA.
In this paper transcriptional factor IIIA (TFIIIA) has been used as a probe for identity of three-dimensional-structure of eukaryotic 5S rRNAs. I was interested in finding a common motif in plant and Xenopus 5S rRNAs for TFIIIA recognition. I found that the two eukaryotic 5S rRNAs (from wheat germ and lupin seeds) are recognized by X. laevis TFIIIA and the data clearly suggest that these 5S rRNAs have very similar if not identical three-dimensional structures. Also effects of various conditions on stability of these complexes have been studied.
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
M Z
MZ
Barciszewska
D000818:Animals; D001483:Base Sequence; D015335:Molecular Probes; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000737:chemistry*; D014982:Xenopus laevis
D015335:Molecular Probes; D012341:RNA, Ribosomal, 5S; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors