Different requirements of CD3 cross-linkage for the activation of memory and naive CD8+ T cells.
In the present experiments, the requirement of CD3 cross-linkage was studied in terms of activation of naive and Ag-primed CD8+ T cells in a murine system. The results showed that Ag-primed CD8+ T cells and both monoclonal and polyclonal T cell lines produced IL-2 by Fab anti-CD3 stimulation without cross-linkage in the absence of accessory cells. In contrast, naive T cells did not produce IL-2 in response to Fab anti-CD3 without participation of accessory cells; however, they produced IL-2 in the presence of accessory cells. These results indicate that the cross-linkage of CD3 molecules is not necessarily required for both naive and memory CD8+ T cells, but naive T cells, not memory T cells, require some interaction with accessory cells for IL-2 production in response to soluble monovalent anti-CD3.
Department of Allergology, Institute of Medical Science, Tokyo, Japan.
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Yee
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Kato
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Tamura
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Nariuchi
D000818:Animals; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000502:physiology; D015152:Blotting, Northern; D017252:CD3 Complex / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000276:immunology; D013154:Spleen / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D016827:CD8 Antigens; D007140:Immunoglobulin Fab Fragments; D007376:Interleukin-2; D017260:Receptor-CD3 Complex, Antigen, T-Cell