Homologous RNA recombination allows efficient introduction of site-specific mutations into the genome of coronavirus MHV-A59 via synthetic co-replicating RNAs.
We describe a novel strategy to site-specifically mutagenize the genome of an RNA virus by exploiting homologous RNA recombination between synthetic defective interfering (DI) RNA and viral RNA. Marker mutations introduced in the DI RNA were replaced by the wild-type residues during replication. More importantly, however, these genetic markers were introduced into the viral genome; even in the absence of positive selection, MHV recombinants were isolated. This finding provides new prospects for the study of coronavirus replication using recombinant DNA techniques. As a first application, we describe the rescue of the temperature sensitive mutant MHV Albany-4 using DI-directed mutagenesis. Possibilities and limitations of this strategy are discussed.
de Groot
R
R
Leiden University, Faculty of Medicine, Department of Virology, The Netherlands.
Heijnen
L
L
van der Most
R
R
Spaan
W
W
D001483:Base Sequence; D003673:Defective Viruses / Q000235:genetics; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000235:genetics* / Q000254:growth & development; D016297:Mutagenesis, Site-Directed / Q000235:genetics*; D017354:Point Mutation; D012367:RNA, Viral / Q000096:biosynthesis / Q000138:chemical synthesis; D011995:Recombination, Genetic; D017423:Sequence Analysis, RNA; D014779:Virus Replication
D012367:RNA, Viral