[Antimutagenic and bactericidal substances in the fruit body of a Basidiomycete Agaricus blazei, Jun-17].
The fruit body of a Basidiomycete Agaricus blazei, Jun-17 (Himematsutake) was extracted with hexane and chloroform-methanol (2:1, v/v), and the antimutagenic effect of the extracts was examined using an Ames/Salmonella/microsome assay. Both extracts of Agaricus inhibited the mutagenicity of benzo[a]pyrene(B[a]P). The hexane extract was purified by silica gel column chromatography and high performance liquid chromatography (HPLC), and linoleic acid was isolated as a main substance having antimutagenic activity. Fr. IIa, IIb, IIc and IIb, which reduced the number of His+ revertant colonies induced by B[a]P, were separated from the chloroform-methanol extract by silica gel column chromatography and HPLC. An antimutagenic substance in Fr. IIa was linoleic acid. From Fr. IIb, a bactericidal, not antimutagenic, substance was isolated and identified as 13-hydroxy cis-9, trans-11-octadecadienoic acid (13ZE-LOH). Antimutagenic substances in Fr. IIc and IId were not purified. The possible source and mechanism of formation of 13ZE-LOH are discussed.
Tokyo College of Pharmacy, Japan.
Y
Y
Osaki
T
T
Kato
K
K
Yamamoto
J
J
Okubo
T
T
Miyazaki
D000364:Agaricus / Q000737:chemistry*; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000302:isolation & purification / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects*
D016587:Antimutagenic Agents; D008041:Linoleic Acids; C024348:13-hydroxy-9,11-octadecadienoic acid; D019787:Linoleic Acid