Primary structure of the major glycan from human seminal transferrin.
Human seminal transferrin (HSmT) is an iron-containing glycoprotein whose structural properties have not been adequately investigated. The carbohydrate content of the purified glycoprotein amount to 6.1%, and monosaccharide analysis revealed the major oligosaccharide moiety to be of the N-glycoside type. The carbohydrate chains were released from the iron-free form by digestion with peptide N-glycosidase F (PNGase F) in the presence of detergents such as SDS and beta-octylglucoside. After ethanol precipitation and fractionation on Bio-Gel P-6 and Bio-Gel P-2, the oligosaccharide was further purified on Mono-Q and desalted on Bio-Gel P-2. By 600-MHz 1H-NMR spectroscopy, the primary structure of the major N-linked oligosaccharide component was established to be: [formula: see text]
D'Andrea
G
G
Department of Sciences and Biomedical Technologies and Biometrics, University of L'Aquila, Italy.
D'Alessandro
A M
AM
Salucci
M L
ML
Oratore
A
A
D000581:Amidohydrolases; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003902:Detergents; D006801:Humans; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012661:Semen / Q000737:chemistry*; D014168:Transferrin / Q000737:chemistry* / Q000302:isolation & purification
D003902:Detergents; D009844:Oligosaccharides; D011134:Polysaccharides; D014168:Transferrin; D006859:Hydrogen; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase