Effects of storage time and temperature on ionized calcium concentration in bovine and ovine blood, plasma, and serum.
Stability of ionized calcium (Ca2+) concentration and pH values in bovine and ovine venous samples (n = 12 in each group) stored at 4 C for 3, 9, 24, and 48 hours (blood, plasma, and serum) or for 240 hours (plasma and serum), and at -20 C for 240 hours (plasma and serum), was studied. Storage of bovine and ovine blood, plasma, and serum samples at 4 C for up to 48 hours and of ovine serum samples at 4 C for up to 240 hours, despite appreciable pH changes, was associated with < 2.0% change in blood, plasma, and serum Ca2+ concentrations. Therefore, Ca2+ concentration in bovine and ovine venous blood, plasma, and serum samples stored up to 48 hours, and in ovine serum samples stored up to 240 hours, at 4 C is of diagnostic use.
Szenci
O
O
Department of Obstetrics and Reproduction, Faculty of Veterinary Science, University of Budapest, Hungary.
Németh
F
F
Stollár
Z
Z
Brydl
E
E
D000818:Animals; D001793:Blood Preservation / Q000662:veterinary*; D002118:Calcium / Q000097:blood*; D002417:Cattle / Q000097:blood*; D005260:Female; D006863:Hydrogen-Ion Concentration; D010949:Plasma / Q000737:chemistry; D011247:Pregnancy; D012016:Reference Values; D012756:Sheep / Q000097:blood*; D013696:Temperature; D013997:Time Factors; D014680:Veins
D002118:Calcium