Lymphocyte and neuronal antigens in neuropsychiatric lupus: presence of an elutable, immunoprecipitable lymphocyte/neuronal 52 kd reactivity.
OBJECTIVE
To examine specific lymphocyte or neuronal antigens immuno-precipitated by systemic lupus erythematosus (SLE) sera.


METHODS
SLE sera were screened for the presence of antibodies binding to surface antigens of CD4(+) HUT-78 or SK-N-SH and IMR-6 neuroblastoma cells using Western blotting or radioimmunoprecipitation.


RESULTS
IgG eluates from both lymphocytes and neuroblastoma cells recognised a 52 kd band in HUT 78 cell lysates. Eight sera studied further using radioimmunoprecipitation also demonstrated binding to a 52 kd antigen (4/8 on HUT-78, 8/8 on SK-N-SH cells), partially depleted by absorption with viable HUT-78.


CONCLUSIONS
A 52 kd antigen recognised by SLE sera on lymphocytes and neuronal cells may play a role in the pathogenesis of neuropsychiatric-SLE.
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
J A
JA
Denburg
S A
SA
Behmann
D000954:Antigens, Surface / Q000032:analysis*; D015153:Blotting, Western; D002478:Cells, Cultured; D003429:Cross Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D001523:Mental Disorders / Q000276:immunology; D009422:Nervous System Diseases / Q000209:etiology / Q000276:immunology*; D009474:Neurons / Q000276:immunology*; D015531:Radioimmunoprecipitation Assay
D000954:Antigens, Surface; D007074:Immunoglobulin G