Purification and some properties of a Vero toxin 2 (Shiga-like toxin II) variant (SLT-IIva) of Escherichia coli isolated from infantile diarrhea.
A variant of Vero toxin 2 (VT2), or Shiga-like toxin II (SLT-II) was purified from a cloned strain of Escherichia coli carrying a gene that encodes SLT-IIva, which is most closely related to SLT-IIv (VT2vp, VTe) in its nucleotide sequence. The purified SLT-IIva showed a slight difference from the purified SLT-IIv (VT2vp) in mobility on polyacrylamide gel disc electrophoresis and SDS-polyacrylamide gel slab electrophoresis and in pI, but both showed a similar potency in biological activities. Ouchterlony double diffusion test revealed that the purified SLT-IIva and SLT-IIv (VT2vp) formed a spur against anti-SLT-IIva and anti-SLT-IIv (VT2vp) antisera.
Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.
M
M
Ohmura
C
C
Cao
T
T
Karasawa
J
J
Okuda
H
H
Kurazono
V P
VP
Gannon
C L
CL
Gyles
Y
Y
Takeda
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification / Q000633:toxicity*; D003968:Diarrhea, Infantile / Q000382:microbiology*; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D005779:Immunodiffusion; D007223:Infant; D051379:Mice; D022641:Shiga Toxin 2; D013552:Swine; D014709:Vero Cells / Q000187:drug effects
D001427:Bacterial Toxins; D022641:Shiga Toxin 2