NaCl regulation of plasma membrane H(+)-ATPase gene expression in a glycophyte and a halophyte.
NaCl regulation of plasma membrane H(+)-ATPase gene expression in the glycophyte tobacco (Nicotiana tabacum L. var Wisconsin 38) and the halophyte Atriplex nummularia L. was evaluated by comparison of organ-specific mRNA abundance using homologous cDNA probes encoding the ATPases of the respective plants. Accumulation of mRNA was induced by NaCl in fully expanded leaves and in roots but not in expanding leaves or stems. The NaCl responsiveness of the halophyte to accumulate plasma membrane H(+)-ATPase mRNA in roots was substantially greater than that of the glycophyte. Salt-induced transcript accumulation in A. nummularia roots was localized by in situ hybridization predominantly to the elongation zone, but mRNA levels also increased in the zone of differentiation. Increased message accumulation in A. nummularia roots could be detected within 8 h after NaCl (400 mM) treatment, and maximal levels were severalfold greater than in roots of untreated control plants. NaCl-induced plasma membrane H(+)-ATPase gene expression in expanded leaves and roots presumably indicates that these organs require increased H(+)-electrochemical potential gradients for the maintenance of plant ion homeostasis for salt adaptation. The greater capacity of the halophyte to induce plasma membrane H(+)-ATPase gene expression in response to NaCl may be a salt-tolerance determinant.
Niu
X
X
Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, Indiana 47907-1165.
Narasimhan
M L
ML
Salzman
R A
RA
Bressan
R A
RA
Hasegawa
P M
PM
D002462:Cell Membrane / Q000201:enzymology; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D017343:Genes, Plant; D017403:In Situ Hybridization; D007700:Kinetics; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010944:Plants / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D010947:Plants, Toxic; D006180:Proton-Translocating ATPases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D012965:Sodium Chloride / Q000494:pharmacology*; D013045:Species Specificity; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
D012333:RNA, Messenger; D012965:Sodium Chloride; D006180:Proton-Translocating ATPases