Analysis of clones carrying repeated DNA sequences in two YAC libraries of Arabidopsis thaliana DNA.
YAC clones carrying repeated DNA sequences from the Arabidopsis thaliana genome have been characterized in two widely used Arabidopsis YAC libraries, the EG library and the EW library. Ribosomal, chloroplast and the paracentromeric repeat sequences are differentially represented in the two libraries. The coordinates of YAC clones hybridizing to these sequences are given. A high proportion of EG YAC clones were classified as containing chimaeric inserts because individual clones carried unique sequences and repetitive sequences originating from different locations in the genome. None of the EW YAC clones analysed were chimaeric in this way. YAC clones carrying tandemly repeated sequences, such as the paracentromeric or rDNA sequences, exhibited a high degree of instability. These observations need to be taken into account when using these libraries in the development of a physical map of the Arabidopsis genome and in chromosome walking experiments.
Department of Molecular Genetics, Cambridge Laboratory, AFRC Institute of Plant Science Research, John Innes Centre, Colney, Norwich, UK.
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R
Schmidt
J
J
Putterill
J
J
West
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G
Cnops
F
F
Robson
G
G
Coupland
C
C
Dean
D017360:Arabidopsis / Q000235:genetics*; D002678:Chimera; D002736:Chloroplasts / Q000378:metabolism; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D015247:Deoxyribonuclease HindIII; D006570:Heterochromatin; D012091:Repetitive Sequences, Nucleic Acid
D004275:DNA, Ribosomal; D006570:Heterochromatin; D015247:Deoxyribonuclease HindIII