Responsiveness of the adult cricket (Gryllus bimaculatus and Acheta domesticus) retrocerebral complex to allatostatin-1 from a cockroach, Diploptera punctata.
Brain-retrocerebral complexes of female crickets, Gryllus bimaculatus and Acheta domesticus, treated with antibody to allatostatin-1 from a cockroach, Diploptera punctata, show extensive immunoreactivity. The results suggest that allostatins or allatostatin-like molecules are produced in neurosecretory cells of the brain and are delivered to the corpora allata through nervous connections and/or via haemolymph. Radiochemical measurements of juvenile hormone III biosynthesis by isolated corpora cardiaca-corpora allata complexes from adult G. bimaculatus have been used to demonstrate an in vitro sensitivity of these glands to allatostatin-1 from D. punctata. Allatostatin-1 is a relatively potent inhibitor of juvenile hormone III biosynthesis in corpora allata of both young adult females and males. In glands taken from 3-day virgin females, 50% inhibition of hormone biosynthesis is reached at ca. 3 nmol.l-1 allatostatin-1. The inhibitory action of allatostatin-1 is rapid, dose-dependent and reversible. Addition of 200 mumol.l-1 farnesol to the incubation medium prevents inhibition of juvenile hormone III biosynthesis by allatostatin-1. Juvenile hormone III biosynthesis by isolated corpora allata of 3-day female house crickets, A. domesticus, is also susceptible to inhibition by 1 mumol.l-1 allatostatin-1.
Abteilung Allgemeine Zoologie, Universität Ulm, Germany.
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D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D003058:Cockroaches; D003335:Corpora Allata / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005204:Farnesol / Q000494:pharmacology; D005260:Female; D006135:Gryllidae / Q000187:drug effects*; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000494:pharmacology*; D007605:Juvenile Hormones / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology*; D014018:Tissue Distribution
D007301:Insect Hormones; D007605:Juvenile Hormones; D009479:Neuropeptides; C061164:allatostatin 1; D005204:Farnesol