Interleukin-1 receptor antagonist prevents low dose streptozotocin induced diabetes in mice.
The effect of the interleukin-1 receptor antagonist (IL-1ra) on development of hyperglycemia and insulitis in mice treated with multiple low doses of streptozotocin (STZ) was evaluated. C57BL/Ks mice were subjected to the following treatments: 1) Injections i.p. of phosphate-buffered saline (PBS) alone; 2) STZ (5 x 40 mg/kg bw); 3) STZ + PBS delivered by an osmotic pump implanted s.c; 4) STZ + IL-1ra delivered by an osmotic pump (approximately 8 mg/kg bw) for 12-14 days starting on day 5, the last day of STZ injection. IL-1ra had a clear protective action against hyperglycemia and insulitis until day 19, i.e., on cessation of IL-1ra administration. Thus, the results indicate that sustained administration of IL-1ra can prevent the diabetogenic process elicited by low dose STZ treatment, suggesting that IL-1 may have a role in the pathogenesis of this form of diabetes.
Department of Medical Cell Biology, Uppsala University, Sweden.
J O
JO
Sandberg
A
A
Andersson
D L
DL
Eizirik
S
S
Sandler
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D053590:Interleukin 1 Receptor Antagonist Protein; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D012016:Reference Values; D012795:Sialoglycoproteins / Q000494:pharmacology*; D013311:Streptozocin / Q000008:administration & dosage; D013997:Time Factors
D001786:Blood Glucose; C507764:Il1rn protein, mouse; D007328:Insulin; D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D013311:Streptozocin