Single-strand conformation polymorphism (SSCP) analysis of the COL3A1 gene detects a mutation that results in the substitution of glycine 1009 to valine and causes severe Ehlers-Danlos syndrome type IV.
A single base mismatch was detected by single-strand conformation polymorphism (SSCP) of the collagen type III gene in a patient with Ehlers-Danlos syndrome type IV. The patient's fibroblasts secreted both normal and slowly migrating type III procollagen molecules. Two-dimensional CNBr peptide mapping suggested that the defect was localised in the CB9 peptide or the C-propeptide region of the alpha 1 (III)-chain. Analysis of a set of restriction-endonuclease-digested fragments of an amplified cDNA sequence encoding CB9, identified a single-strand conformation polymorphism and localized it within a region of 79 bp corresponding to the carboxyl-terminal end of the CB9 peptide of the alpha 1(III)-chain. DNA sequence analysis demonstrated that the patient was heterozygous for a point mutation converting G to T at base pair 3440 of the collagen alpha 1(III) cDNA resulting in the substitution of glycine with valine at amino acid position 1009 of the alpha 1(III)-chain. The mutation in this patient lies within a region of mutations at the carboxyl-terminal end of the type III collagen alpha-helix which all produce a severe "acrogeric" form of EDS IV.
Nuytinck
L
L
Centre for Medical Genetics, University Hospital Gent, Belgium.
De Paepe
A
A
Renard
J P
JP
Adriaens
F
F
Leroy
J
J
D000293:Adolescent; D000595:Amino Acid Sequence; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis / Q000235:genetics*; D004535:Ehlers-Danlos Syndrome / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005821:Genetic Techniques; D005998:Glycine; D006801:Humans; D008297:Male; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011347:Procollagen / Q000032:analysis / Q000235:genetics*; D015183:Restriction Mapping; D012867:Skin / Q000378:metabolism; D014633:Valine
D011347:Procollagen; D003094:Collagen; D014633:Valine; D005998:Glycine