Formation of F2-isoprostanes during aortic endothelial cell-mediated oxidation of low density lipoprotein.
We investigated the formation of F2-isoprostanes produced by non-enzymatic peroxidation of arachidonic acid during rabbit aortic endothelial cell-mediated oxidation of low density lipoprotein (LDL). Free and total (sum of free and esterified) levels of F2-isoprostanes were measured using a solid-phase extraction procedure and gas chromatography-mass spectrometry. Free levels of F2-isoprostanes in native LDL were 0.06 +/- 0.03 ng/mg protein (n = 4), whereas total levels were 0.28 +/- 0.09 ng/mg protein (n = 4). Both free and total levels of the isoprostanes were found to increase during the oxidation. 8-epi-PGF2 alpha was the major isoprostane formed (free and total concentrations after 24 h, 2.50 +/- 0.24 and 6.42 +/- 1.36 ng/mg protein (n = 4), respectively). The release of F2-isoprostanes during aortic endothelial cell-induced oxidation of LDL could be a contributory factor in the development of atherosclerosis.
William Harvey Research Institute, St Bartholomew's Hospital Medical College, London, UK.
N K
NK
Gopaul
J
J
Nourooz-Zadeh
A I
AI
Mallet
E E
EE
Anggård
D000818:Animals; D001011:Aorta / Q000378:metabolism; D002470:Cell Survival; D015237:Dinoprost / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000378:metabolism*; D007700:Kinetics; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction; D011817:Rabbits; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; D015237:Dinoprost