Placental lactogen and somatotropin: hormone binding to the corpus luteum and effects on the growth and functions of the ovary in heifers.
The effects of recombinant bovine placental lactogen (rbPL) and recombinant bovine somatotropin (rbST) on development of ovarian follicles and CL were tested in heifers. Estrus (day = 0) was synchronized and heifers were treated (Days 0-21) with either saline (control; n = 7), rbST (25 mg/day; n = 6), or rbPL. (50 mg/day; n = 8). Blood was collected daily for analyses of progesterone, estradiol, ST, PL, and insulin-like growth factor (IGF)-I; ultrasound was performed daily for measurement of follicles and CL. PL in plasma (mean +/- SE; ng/ml) averaged 4.1 +/- 0.2 for rbPL-treated heifers, and ST in plasma (ng/ml) averaged 2.7 +/- 0.3 for rbST-treated heifers. IGF-I in plasma (ng/ml) was increased for rbST-treated (198 +/- 10; p < 0.001) and rbPL-treated (143 +/- 9; p < 0.06) heifers compared to controls (117 +/- 9). After Day 9 of the estrous cycle, heifers treated with rbPL had larger CL (p < 0.001) and more progesterone in plasma (p < 0.001) than controls, whereas rbST-treated heifers were intermediate for these measures. Largest follicles were decreased in size (mm) throughout the estrous cycle for rbPL-treated heifers (12.9 +/- 0.4) compared to controls (14.2 +/- 0.5; p < 0.06) or heifers given rbST (14.0 +/- 0.5; p < 0.11). After Day 17 (preovulatory period), concentrations of estradiol in serum (pg/ml) were decreased for rbST-treated (2.7 +/- 0.3; p < 0.01) and rbPL-treated (2.9 +/- 0.2; p < 0.02) heifers compared to controls (3.8 +/- 0.3).(ABSTRACT TRUNCATED AT 250 WORDS)
Lucy
M C
MC
Animal Sciences Division, Monsanto Company, St. Louis, Missouri 63198.
Byatt
J C
JC
Curran
T L
TL
Curran
D F
DF
Collier
R J
RJ
D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000033:anatomy & histology / Q000502:physiology*; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000502:physiology; D005260:Female; D013006:Growth Hormone / Q000378:metabolism* / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000502:physiology; D010053:Ovary / Q000502:physiology*; D010060:Ovulation / Q000502:physiology; D010928:Placental Lactogen / Q000378:metabolism* / Q000494:pharmacology; D011374:Progesterone / Q000097:blood; D011869:Radioligand Assay; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D010928:Placental Lactogen