Characterization of a cDNA and gene encoding a cuticular protein from rigid cuticles of the giant silkmoth, Hyalophora cecropia.
We have isolated a cDNA and gene encoding a protein (HCCP66) found in the rigid cuticles of both larvae and pupae of the silkmoth, Hyalophora cecropia. The cDNA encoded a protein similar to cuticle proteins isolated from several other insects and contained a sequence motif similar to one present in a "family" of cuticular proteins from flexible cuticles. The gene had a structure similar to that of cuticle protein genes isolated from Drosophila melanogaster, albeit with a much larger intron that contained three copies of a transposable element-like sequence similar to short interspersed repeated DNA elements (SINEs). A sequence found 5' to the transcription start site matched the Octamer (Oct) cis-acting element. This sequence was capable of binding protein(s) from whole cell extracts of wing epidermis with high affinity and sequence specificity suggesting a role in transcriptional regulation.
Lampe
D J
DJ
Department of Entomology, University of Illinois, Urbana 61801.
Willis
J H
JH
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000378:metabolism; D018076:DNA, Complementary; D015723:Gene Library; D017344:Genes, Insect / Q000235:genetics*; D019476:Insect Proteins; D007814:Larva / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011679:Pupa / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014921:Wings, Animal / Q000737:chemistry
D000596:Amino Acids; C088240:CP66 protein, Hyalophora cecropia; D018076:DNA, Complementary; D019476:Insect Proteins; D011506:Proteins; D012333:RNA, Messenger; D004247:DNA