Effect of prenatal dexamethasone administration: fetal rabbit intestinal nutrient uptake and disaccharidase development.
To examine the effect of prenatal steroids on fetal intestinal maturation, eight pregnant rabbits received either dexamethasone (Dex) or saline (Cont) on Days 25-27 of a 31-day gestation. As the rabbit provides a model of growth retardation based on uterine position, fetuses were identified as favored (Fav) or runt (Runt), generating four study groups: ContFav, ContRunt, DexFav, and DexRunt. On Day 31 the small intestinal uptake of glucose and proline was measured by an everted sleeve technique. Additionally, lactase and maltase activity was determined. Small intestinal length and nutrient uptake was significantly increased in the Dex fetuses. Control runts had a trend to decreased levels of nutrient uptake when compared to their favored counterparts. This trend reversed in the Dex fetuses with runt nutrient uptake surpassing that of the favored fetus. A trend to increased enzyme activity of both lactase and maltase was demonstrated. This report provides the first description of maternal steroid administration causing a marked increase in fetal small intestinal length and glucose and proline absorption in an in vivo model of intrauterine growth retardation.
Department of Surgery, UCLA School of Medicine 90024.
T L
TL
Buchmiller
K S
KS
Shaw
M L
ML
Lam
R
R
Stokes
J S
JS
Diamond
E W
EW
Fonkalsrud
D000704:Analysis of Variance; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology* / Q000627:therapeutic use; D004186:Disaccharidases / Q000378:metabolism; D004195:Disease Models, Animal; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D004761:Enterocolitis, Pseudomembranous / Q000517:prevention & control; D005260:Female; D005317:Fetal Growth Retardation / Q000188:drug therapy / Q000378:metabolism; D005325:Fetal Organ Maturity; D005947:Glucose / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects*; D007421:Intestine, Small / Q000196:embryology* / Q000201:enzymology; D008431:Maternal-Fetal Exchange; D008871:Microvilli / Q000502:physiology; D011247:Pregnancy; D011392:Proline / Q000378:metabolism; D011817:Rabbits
D003907:Dexamethasone; D011392:Proline; D004186:Disaccharidases; D005947:Glucose