Arachidonate metabolism in ischemia-reperfusion associated with pancreas transplantation.
The implication of eicosanoid metabolism and its relationship with oxygen free radical production in the process of ischemia-reperfusion associated with rat pancreas transplantation has been explored in this study. For this purpose male Sprague-Dawley rats were classified as follows: group I, control animals not surgically manipulated; group II, pancreas transplantation, after 30 min preservation in UW solution; group III, pancreas transplantation after 12 h preservation under the same conditions; group IV, same as group III but with administration of SOD 5 min prior to organ revascularization. The results show post-transplantation increases in 6-keto-PGF1 alpha, TXB2, LTB4 and 12-HETE in pancreatic tissue independent of preservation time. The fact that SOD administration could reverse these increases even though an efficient xanthine oxidase irreversible inhibitor such as allopurinol was present in the preservation solution suggests that eicosanoid generation in the recipient rat would be mediated by an oxygen free radical dependent mechanism not exclusively dependent on endothelial xanthine oxidase activity.
Hotter
G
G
Department of Neurochemistry, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.
Closa
D
D
Pi
F
F
Roselló-Catafau
J
J
Bulbena
O
O
Badosa
F
F
Fernández-Cruz
L
L
Gelpí
E
E
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D003080:Cold Temperature; D005609:Free Radicals; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D007511:Ischemia; D007975:Leukotriene B4 / Q000378:metabolism; D008297:Male; D009926:Organ Preservation; D010179:Pancreas / Q000098:blood supply / Q000378:metabolism; D016035:Pancreas Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D013482:Superoxide Dismutase / Q000494:pharmacology; D013929:Thromboxane B2 / Q000378:metabolism
D005609:Free Radicals; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D016718:Arachidonic Acid; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D013482:Superoxide Dismutase