Use of signal-distinguishable probes in differential or sequential autoradiography in hybridization analysis.
To avoid the time-consuming reprobing process in hybridization analysis, signal-distinguishable probes (32P, 35S or antigenic hapten-labeled DNA) can be added to the same hybridization mixture. After hybridization, an unambiguous result can be obtained by differential or sequential autoradiography.
Au
L C
LC
Veterans General Hospital, Taipei, Taiwan.
Chang
K J
KJ
Shih
C M
CM
Teh
G W
GW
D000199:Actins / Q000235:genetics; D001345:Autoradiography; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D004076:Digoxigenin; D005260:Female; D005914:Globins / Q000235:genetics; D006801:Humans; D007553:Isotope Labeling; D008113:Liver Neoplasms; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization / Q000379:methods*; D027383:Papillomaviridae / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013462:Sulfur Radioisotopes; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000382:microbiology
D000199:Actins; D015342:DNA Probes; D004279:DNA, Viral; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D005914:Globins; D004076:Digoxigenin