Isolation of two species of Chlamydomonas reinhardtii flagellar mutants, ida5 and ida6, that lack a newly identified heavy chain of the inner dynein arm.
Two novel Chlamydomonas mutants, ida5 and ida6, that lack subsets of inner-arm dynein have been isolated and mapped to discrete loci on the right arm of linkage group XIV. Of the seven different inner-arm dynein subspecies (a, b, c, d, e, f and g) identified by ion-exchange chromatography, ida5 lacks a, c, d and e, while ida6 lacks e alone; these are the only mutants that have been shown to lack subspecies e. Both strains can swim, albeit more slowly than the wild type. Hence, subspecies e must contribute to flagellar movement although it is unnecessary for the generation of undulating movement.
Department of Molecular Biology, School of Science, Nagoya University, Japan.
T
T
Kato
O
O
Kagami
T
T
Yagi
R
R
Kamiya
D000818:Animals; D002465:Cell Movement / Q000235:genetics; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000302:isolation & purification; D002874:Chromosome Mapping; D004398:Dyneins / Q000737:chemistry / Q000235:genetics*; D005407:Flagella / Q000648:ultrastructure*; D008040:Genetic Linkage; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics*; D013329:Structure-Activity Relationship
D010446:Peptide Fragments; D004398:Dyneins