Tumor necrosis factor alpha, a cytokine coregulating sugar uptake by cultured human synovial cells.
Confluent cultures of osteoarthritic and rheumatoid human synovial cells were treated with human recombinant tumor necrosis factor alpha (TNF-alpha). The cytokine increased uptake of 2-deoxy-D-[1-3H]-glucose (2-DOG) in a time- and concentration-dependent manner. In synovial cells obtained from osteoarthritic patients (OA cells), the stimulation of 2-DOG uptake occurred 3 hours following addition of TNF-alpha (1 ng/ml) and was maximal by 24 hours. Rheumatoid synovial cells (RA cells) appeared less sensitive to the cytokine: 2-DOG uptake stimulation was only significant after 6 hours of incubation. In both OA and RA cells, the effect was protein synthesis-dependent, and was not secondary to prostaglandin E2 synthesis or cell growth. Interleukin-1 beta was more efficient than TNF-alpha for 2-DOG uptake stimulation. The two cytokines seemed to act in an additive manner.
Laboratoire de Biochimie Cellulaire de l'Inflammation, Université Paris XI, Chatenay-Malabry, France.
A
A
Hernvann
C
C
Souliac-Marc
C
C
Aussel
O
O
Ekindjian
D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism / Q000493:pharmacokinetics*; D015232:Dinoprostone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D010003:Osteoarthritis / Q000378:metabolism / Q000473:pathology; D011994:Recombinant Proteins / Q000494:pharmacology; D013583:Synovial Membrane / Q000166:cytology* / Q000378:metabolism*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D007375:Interleukin-1; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D003513:Cycloheximide; D003847:Deoxyglucose; D015232:Dinoprostone; D007213:Indomethacin