Prostaglandin E1 protects dog pancreas from ischemia-reperfusion injury.
Effects of prostaglandin (PG) E1 on ischemia-reperfusion (I-R) injury to the pancreas was evaluated using isolated in vivo perfused dog pancreas. Pancreatic endocrine and exocrine functions were stimulated with 10(-12) M cholecystokinin octapeptide (CCK-8). This amount of CCK-8 promoted production of insulin, glucagon, PGI2, and thromboxane (Tx) A2 in the pancreas. Sixty minutes of ischemia and subsequent reperfusion induced damage to pancreatic ductular, acinar, and beta cells. Intra-arterial administration of PGE1 at a dose of 0.5 microgram/kg/min throughout the experiment prevented the I-R injury, reducing plasma lipid peroxides, and elevating PGI2 without changing TxA2 in the pancreas. PGE1 thus appears to protect pancreatic function from I-R injury both by depressing the effect of free-radicals and by decreasing TxA2/PGI2 which predicts cell injury.
Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
T
T
Kuroda
E
E
Shiohara
Y
Y
Haba
K
K
Hanazaki
T
T
Homma
K
K
Iwatsuki
S
S
Chiba
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D004285:Dogs; D005260:Female; D005934:Glucagon / Q000097:blood; D007328:Insulin / Q000097:blood; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D010179:Pancreas / Q000098:blood supply / Q000187:drug effects*; D015427:Reperfusion Injury / Q000517:prevention & control*; D013929:Thromboxane B2 / Q000097:blood
D007328:Insulin; D008054:Lipid Peroxides; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D005934:Glucagon; D000527:Alprostadil