Chromosomal localization of human RNA polymerase II subunit genes.
The eukaryotic DNA-dependent RNA polymerase II (or B) is composed of 10 to 14 polypeptides ranging from 220 to 10 kDa. To gain further insight into the molecular structure and function of these subunits, we have undertaken the molecular cloning of nucleotide sequences corresponding to the human enzyme. The cDNAs of five subunits (hRPB220, hRPB140, hRPB33, hRPB25, and hRPB14.5) have been isolated. Using in situ hybridization, we show that the genes of these subunits have distinct chromosomal locations (17p13, 4q12, 16q13-q21, 19p13.3, and 19q12, respectively). Thus, if assembly of active polymerase molecules requires coordinated expression from these independent genes, mechanisms that ensure tight coregulation of the corresponding promoters must exist.
Laboratoire de Génétique Moléculaire des Eucaryotes (CNRS), Unité 184 (INSERM), Strasbourg, France.
J
J
Acker
M G
MG
Mattei
M
M
Wintzerith
N
N
Roeckel
D
D
Depetris
M
M
Vigneron
C
C
Kedinger
D002871:Chromosome Banding; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D002880:Chromosomes, Human, Pair 11; D002885:Chromosomes, Human, Pair 16; D002886:Chromosomes, Human, Pair 17; D002888:Chromosomes, Human, Pair 19; D002894:Chromosomes, Human, Pair 4; D015342:DNA Probes; D005091:Exons; D006801:Humans; D017403:In Situ Hybridization; D046911:Macromolecular Substances; D012319:RNA Polymerase II / Q000235:genetics*
D015342:DNA Probes; D046911:Macromolecular Substances; D012319:RNA Polymerase II