A comparison of the effects of human rIL-2 and autologous TCGF on Xenopus laevis splenocytes in vitro.
Prior studies have shown that intraperitoneal (ip) injection of 25 IU of human rIL-2 can effectively modulate in vivo immune reactivity to thymus-dependent and thymus-independent type 2 immunogens in Xenopus laevis, the South African clawed toad, but is less successful at affecting toad cells in vitro. Here we compare the capacities of human rIL-2 and autologous TCGF to modulate Xenopus splenocytes in vitro and find that autologous TCGF (1) is more effective at stimulating mitogenesis, (2) can serve as a ligand for inducible receptors that will also bind rIL-2 and an F1*-mouse anti-human p55 antibody, and (3) will regulate the expression these receptors.
Ruben
L N
LN
Department of Biology, Reed College, Portland, Oregon 97202.
Rak
J C
JC
Johnson
R O
RO
Shiigi
S
S
Clothier
R H
RH
D000818:Animals; D001667:Binding, Competitive; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D008934:Mitogens / Q000494:pharmacology*; D010802:Phylogeny; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D014982:Xenopus laevis / Q000276:immunology*
D007376:Interleukin-2; D008934:Mitogens; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins