Localization of GABAA receptors in the rabbit retina.
The distribution of gamma-aminobutyric acidA (GABAA) receptors in the rabbit retina is investigated and compared with the distribution of GABAergic neurons using immunocytochemical methods. Antibodies against the alpha 1, beta 2/3, and gamma 2 subunits of the GABAA receptor label subpopulations of bipolar, amacrine and ganglion cells. Double labeling experiments show that the gamma 2 subunit is colocalized with the alpha 1 and the beta 2/3 subunits in bipolar, amacrine and ganglion cells. Electron microscopy reveals that in the outer plexiform layer, GABAA receptor immunoreactivity is present on dendrites of cone bipolar cells adjacent to the cone pedicles. Bipolar cell dendrites are also receptor-positive at synapses from interplexiform cells. Some receptor immunoreactivity is found intracellularly in processes of horizontal cells. In the inner plexiform layer, GABAA receptor immunoreactivity is present on both rod bipolar and cone bipolar axon terminals at putative GABAergic input sites. Amacrine and ganglion cell processes in sublamina a and b are also labeled.
Max-Planck-Institut für Hirnforschung, Frankfurt, Germany.
U
U
Greferath
U
U
Grünert
F
F
Müller
H
H
Wässle
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005136:Eye Proteins / Q000032:analysis*; D008854:Microscopy, Electron; D011817:Rabbits; D018079:Receptors, GABA / Q000032:analysis* / Q000276:immunology; D012160:Retina / Q000737:chemistry* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D005136:Eye Proteins; D018079:Receptors, GABA