Interleukin-1 and interleukin-6 modify protein phosphorylation in the central nervous system of Hirudo medicinalis.
Recent data suggest that IL-1-like molecules have been conserved during evolution. The signal transduction mechanism of IL-1 is not known, but kinase activation has been reported. With the aim of studying if human IL-1 has any effect on the leech nervous system, we have added this cytokine to segmental ganglia labeled with [32P]ortophosphoric acid; proteins have been separated by electrophoresis and phosphoproteins detected by autoradiography. In the present paper we show that human IL-1 and IL-6 are able to induce changes on protein phosphorylation in the leech central nervous system and that these changes are similar to those ones induced by the neurotransmitter serotonin.
Bottai
D
D
Department of Physiology and Biochemistry G. Moruzzi, University of Pisa, Italy.
Garcia-Gil
M
M
Zaccardi
M L
ML
Fineschi
L
L
Brunelli
M
M
D000818:Animals; D002490:Central Nervous System / Q000187:drug effects* / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D007858:Learning / Q000187:drug effects; D007865:Leeches / Q000187:drug effects* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation; D012701:Serotonin / Q000494:pharmacology*
D007375:Interleukin-1; D015850:Interleukin-6; D009419:Nerve Tissue Proteins; D012701:Serotonin