Xenopus laevis oocytes, eggs and tadpoles contain immunoactive insulin.
Insulin is a multifunctional polypeptide hormone that regulates metabolic processes and promotes mitogenesis and differentiation in vitro in the cells and tissues of several species. Its role in vivo during embryogenesis is still poorly understood. We have previously found insulin mRNA in mature Xenopus laevis oocytes and in embryos during neurulation (before organogenesis of the pancreas takes place). We have now measured insulin immunoactivity in mature oocytes, unfertilized eggs and day-2 tadpoles. Using reversed phase high performance liquid chromatography, we found low levels of insulin in extracts of oocytes (stage VI). Both Xenopus insulin I and II were detected in unfertilized eggs. The day-2 tadpoles (stages 31-33) also contained immunoactive insulin, and in swimming tadpoles (stage 46) a few clusters of cells containing insulin immunoactivity could be identified by indirect immunofluorescence. Immunoblot analysis was relatively insensitive, detecting insulin only in the adult Xenopus pancreas. In summary, insulin (from maternal origin and embryonic expression) appears to be present early enough in Xenopus laevis to influence developmental processes such as neurulation.
Diabetes Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892.
F
F
de Pablo
R
R
Dashner
A R
AR
Shuldiner
J
J
Roth
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004625:Embryo, Nonmammalian / Q000737:chemistry*; D005260:Female; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007150:Immunohistochemistry; D007328:Insulin / Q000032:analysis*; D009865:Oocytes / Q000737:chemistry*; D010179:Pancreas / Q000737:chemistry; D011863:Radioimmunoassay; D014982:Xenopus laevis / Q000196:embryology / Q000378:metabolism*; D015053:Zygote / Q000737:chemistry*
D007328:Insulin