Isolation and structural characterization of a novel peptide related to gamma-melanocyte stimulating hormone from the brain of the leech Theromyzon tessulatum.
This paper reports the purification of a novel pro-opiomelanocortin derivative peptide (a gamma-melanocyte stimulating hormone-like (gamma-MSH-like) molecule) from the brain of the leech Theromyzon tessulatum. After reverse-phase HPLC purification, the sequence of the gamma-MSH-like peptide (YVMGHFRWDKFamide) was established by a combination of automated Edman degradation, electrospray mass spectrometry measurement, enzymatic treatment and co-elution experiments in reverse-phase HPLC with synthetic peptides.
Salzet
M
M
Laboratoire de Phylogénie moléculaire des Annélides, ER 87 CNRS, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Wattez
C
C
Bulet
P
P
Malecha
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D006801:Humans; D007865:Leeches; D009074:Melanocyte-Stimulating Hormones / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D010455:Peptides; D009074:Melanocyte-Stimulating Hormones