Cloning of an Arabidopsis histidine transporting protein related to nitrate and peptide transporters.
In plants, many of the proteins involved in transport of nitrogenous compounds have not been identified so far. The use of heterologous complementation in yeast mutants has enabled the isolation of a gene family encoding amino acid permease (AAP). A highly sensitive selection procedure was used to identify other proteins capable of transporting amino acids. In addition to members of the AAP gene family, an integral membrane protein (NTR1) that shows significant similarities to the low affinity nitrate transporter from Arabidpsis and to peptide transporters from yeast and rabbit was identified. NTR1 seems to be involved in the supply of reproductive organs with nitrogen as it is expressed at low levels in leaves and highly in developing pods.
Institut für Genbiologische Forschung, Berlin, Germany.
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WB
Frommer
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S
Hummel
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Rentsch
D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics*; D029681:Arabidopsis Proteins; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D018014:Gene Transfer Techniques; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D026901:Membrane Transport Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D009566:Nitrates / Q000378:metabolism; D033902:Periplasmic Binding Proteins; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017421:Sequence Analysis; D014018:Tissue Distribution
D026905:Amino Acid Transport Systems; D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009566:Nitrates; D033902:Periplasmic Binding Proteins; D010940:Plant Proteins; C018963:histidine-binding protein