Effect of thyrotropin on inositol phospholipids pathway in human thyroid non-toxic goitre.
The present study demonstrates that exposure of human thyroid slices to thyrotropin causes an increase in phospholipid metabolism as measured by an increased incorporation of myo-2-[3H]-inositol into phosphatidylinositol, the generation of InsP3 and formation of GInsP as well as metabolism of phosphatidylcholine and phosphatidic acid. These results indicate that adenosine, probably via the A1 type of P1 receptor, modulates both incorporation of labelled substrate into thyroid slices and its metabolism to active compounds which could play a role in a cell signalling system. These observations confirmed suggestions about the physiological role of the phosphatidylinositol pathway in signalling action of thyrotropin and purinergic receptors in human thyroid.
Department of Physical Chemistry Medical School Białystok, Mickiewicza, Poland.
P
P
Mosko
W
W
Zarzycki
L
L
Jaroszewicz
I
I
Kinalska
D000241:Adenosine / Q000494:pharmacology; D000328:Adult; D005260:Female; D006042:Goiter / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D011983:Receptors, Purinergic / Q000378:metabolism; D015398:Signal Transduction; D013961:Thyroid Gland / Q000502:physiology*; D013972:Thyrotropin / Q000494:pharmacology*
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D011983:Receptors, Purinergic; D007294:Inositol; D013972:Thyrotropin; D000241:Adenosine