[An observation of DNA and RNA within the cells of laryngocarcinoma by acridine orange fluorescent staining].
The shape of cells in groups of 7 individuals with normal laryngeal mucosal epithelium, 12 patients with laryngeal papilloma, 25 with atypical hyperplasia of laryngeal epithelium, 44 with laryngeal squamous cell carcinoma and DNA, RNA were observed by acridine orange fluorescent staining. The result has proved that fluorescent staining is a method of staining, which can be used to show the extent of cell differentiation. As to DNA and RNA within the cells the method has potent specificity and the shape and structure of cells and the change of the content of two kinds of nucleic acids can be demonstrated by means of the method, which is simple, easy and quick to master. The clear video can demonstrate the atypical hyperplasia of laryngeal mucosal epithelium and the changing features of DNA and RNA of laryngocarcinoma, which is of practical value of popularization in early diagnosis of laryngeal malignant tumor.
Dong
X W
XW
Affiliated Hospital of Jinzhou Medical College.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000609:Aminoacridines; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D004848:Epithelium / Q000737:chemistry; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007820:Laryngeal Mucosa / Q000737:chemistry; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology; D012334:RNA, Neoplasm / Q000032:analysis*; D011897:Random Allocation
D000609:Aminoacridines; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D012334:RNA, Neoplasm; C025902:acridine yellow