[Determination of antiphospholipid antibodies in serum using ELISA].
The authors compare two ELISA methods for the assessment of antiphospholipid antibodies, classes IgG and IgM, in serum: ELISA Pin Plate System ALPHA DIALAB Co. and the ELISA method developed in the Research Institute of Rheumatic Diseases. Both methods use cardiolipin as antigen. In the Pin Plate test the immunochemical reaction antigen/antibody does not take place at the surface of the pits of the microtitration plates but on the tip of the next plate. The results of examinations of antiphospholipid antibodies obtained by the tested methods are comparable, the Pin Plate test is quicker and more sensitive, but its price limits routine use.
1. ústav lékarské chemie a biochemie, 1. lékarská fakulta Univerzity Karlovy, Praha.
L
L
Fialová
L
L
Mikulíková
I
I
Malbohan
M
M
Průcha
A
A
Palecková
V
V
Cerný
D017152:Antibodies, Antiphospholipid / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007247:Infertility, Female / Q000276:immunology
D017152:Antibodies, Antiphospholipid; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Stanovení antifosfolipidových protilátek v séru metodami ELISA.