Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp.
Various Streptomyces spp. including S. violaceus, S. scabies, S. griseus, S. exfoliatus, S. coelicolor and S. lividans secrete indole-3-acetic acid (IAA) when fed with L-tryptophan (Trp). Production of IAA was detected in Streptomyces strains causing potato scab as well as in non-pathogenic strains. The pathways for IAA synthesis from Trp were investigated in S. violaceus and S. exfoliatus. Indole-3-acetamide (IAM), indole-3-lactic acid (ILA), indole-3-ethanol (IEt) and IAA were identified by HPLC and GC-MS. Streptomyces cells were capable of catabolizing IAM, ILA, IEt and indole-3-acetaldehyde (IAAId) into IAA. Incorporation of radioactivity into IAM, IAA and ILA but not IEt was detected when cells were fed with L-[3-14C]tryptophan. Results indicate the presence of the IAM pathway (Trp-->IAM-->IAA) and the possible presence of additional pathways for IAA biosynthesis in Streptomyces.
Manulis
Shulamit
S
Shafrir
Hadas
H
Epstein
Ephraim
E
Lichter
Amnon
A
Barash
Isaac
I
D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D007210:Indoleacetic Acids / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism; D008954:Models, Biological; D010935:Plant Diseases / Q000382:microbiology; D013045:Species Specificity; D013302:Streptomyces / Q000378:metabolism* / Q000472:pathogenicity; D014364:Tryptophan / Q000378:metabolism; D014774:Virulence
D007210:Indoleacetic Acids; D007211:Indoles; C030737:indoleacetic acid; C015950:indoleacetamide; D014364:Tryptophan