Platelet-derived growth factor isoforms prevent cell death during starvation of AKR-2B fibroblasts.
Confluent AKR-2B fibroblasts rapidly desintegrate upon removal of serum until a final density of approximately 50% of the initial value was reached after 12 h. This density remained unchanged for at least 48 h. Platelet-derived growth factor (PDGF)-BB stimulated more than 95% of these cells to divide. PDGF-AB or -BB added immediately after serum removal caused complete survival of the cells, but did not stimulate cell division as demonstrated by two-dimensional DNA flow cytometry. PDGF-AA was less effective leading to approximately 75% of the initial cell density. This effect could be augmented by the addition of ocadaic acid, a potent phosphatase inhibitor, suggesting that protein phosphorylation plays a role in this process. By using tyrphostin AG807 it was demonstrated that the signaling mechanism for survival requires receptor tyrosine autophosphorylation.
Department of Physiological Chemistry, Theodor-Boveri-Institute, University of Würzburg, Germany.
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Simm
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Hoppe
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Gazit
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D000818:Animals; D002396:Catechols / Q000494:pharmacology; D016923:Cell Death; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D004988:Ethers, Cyclic / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D009570:Nitriles / Q000494:pharmacology; D019319:Okadaic Acid; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000737:chemistry / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D020032:Tyrphostins
D002396:Catechols; D004988:Ethers, Cyclic; D009570:Nitriles; D010982:Platelet-Derived Growth Factor; D020032:Tyrphostins; C075278:platelet-derived growth factor A; C060152:tyrphostin 47; D019319:Okadaic Acid; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases