Further evidence for clustering of human GABAA receptor subunit genes: localization of the alpha 6-subunit gene (GABRA6) to distal chromosome 5q by linkage analysis.
GABAA receptors are hetero-oligomeric ion-channel complexes that are composed of combinations of alpha, beta, gamma, and delta subunits and play a major role in inhibitory neuro-transmission in the mammalian brain. We report here a microsatellite polymorphism within the human alpha 6-subunit gene (GABRA6). Mapping of this marker in a human-hamster hybrid cell-line panel and typing of the repeat in the Centre d'Etude du Polymorphisme Human (CEPH) reference families enabled the localization of this gene to chromosome 5q and established its linkage to the GABAA receptor alpha 1-subunit gene (GA-BRA1) with a maximum lod score (Zmax) of 39.87 at a theta of 0.069 (males) and 0.100 (females). These results reveal the clustering of GABRA6, GABRA1, and the GABAA receptor gamma 2-subunit gene (GABRG2) on distal chromosome 5q.
Hicks
A A
AA
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, United Kingdom.
Bailey
M E
ME
Riley
B P
BP
Kamphuis
W
W
Siciliano
M J
MJ
Johnson
K J
KJ
Darlison
M G
MG
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D004247:DNA; D004276:DNA, Satellite / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D011110:Polymorphism, Genetic; D018079:Receptors, GABA / Q000737:chemistry / Q000235:genetics*
D004276:DNA, Satellite; D018079:Receptors, GABA; D004247:DNA