Enhancement of rotavirus immunogenicity by microencapsulation.
It was determined whether microencapsulation of rotavirus enhanced virus-specific immunity in mice. Combinations of several water-soluble anionic polymers and amines were tested for their capacity to form microcapsules which were stable in the presence of simulated gastric acid. Using the combinations of sodium alginate and spermine hydrochloride or sodium chondroitin sulfate and spermine hydrochloride we found that microcapsules (1) captured infectious rotavirus, (2) penetrated into the persisted in gut-associated lymphoid tissue (GALT) after oral inoculation, (3) delivered rotavirus antigen to GALT at levels greater than those detected after oral inoculation with free virus, and (4) enhanced the virus-specific humoral immune response after oral or parenteral immunization.
Division of Infectious Diseases, Children's Hospital of Philadelphia, Pennsylvania 19104.
P A
PA
Offit
C A
CA
Khoury
C A
CA
Moser
H F
HF
Clark
J E
JE
Kim
T J
TJ
Speaker
D000588:Amines; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002214:Capsules; D002460:Cell Line; D002522:Chlorocebus aethiops; D004355:Drug Stability; D005744:Gastric Acid / Q000378:metabolism; D007422:Intestines / Q000276:immunology; D008221:Lymphoid Tissue / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011108:Polymers; D012401:Rotavirus / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology* / Q000493:pharmacokinetics
D000588:Amines; D000914:Antibodies, Viral; D002214:Capsules; D011108:Polymers; D014765:Viral Vaccines