Characterization of the B cell-macrophage lineage transition in 70Z/3 cells.
The 70Z/3 cell line is able to undergo lineage conversion from a pre-B to macrophage phenotype. Data presented here show that the transition from pre-B to macrophage follows a reproducible pathway via a stable intermediate stage. The cells in the intermediate stage are adherent and have lost the ability to respond to lipopolysaccharide or interferon-gamma by induction of immunoglobulin kappa light chains. However, these cells do not yet display the full range of macrophage-specific properties such as receptors for macrophage-colony stimulating factor or the beta 2 integrin CD11b/CD18. Subcloning experiments with the intermediate cells revealed that they retain the options of either persisting along the macrophage line of differentiation, acquiring additional macrophage traits, or reverting to the pre-B phenotype. Further differentiation to the macrophage stage is accompanied by the apparent loss of the ability to revert. Thus, these studies define relationships among lineage-specific traits, and begin to reveal critical stages in lineage commitment.
Tanaka
T
T
Wellesley Hospital Research Institute, Toronto, Ontario, Canada.
Wu
G E
GE
Paige
C J
CJ
D000818:Animals; D000911:Antibodies, Monoclonal; D016175:B-Lymphocyte Subsets / Q000737:chemistry / Q000166:cytology*; D015152:Blotting, Northern; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D015870:Gene Expression; D008264:Macrophages / Q000737:chemistry / Q000166:cytology*; D051379:Mice; D051381:Rats
D000911:Antibodies, Monoclonal