[The use of verapamil in tests for determining the drug resistance of leukemic blasts].
To determine prognostically unfavourable groups of acute leukemia patients, the authors studied the in vitro accumulation of 3H-vincristine (Vcr) and adriamycin (ADR) as well as inclusion of 3H-cytosar (Ara-C) into marrow blast DNA from patients showing different effects of treatment. It was found resistant to induction chemotherapy increases with verapamil addition to culture medium (Vrp+ cells). ADR inclusion into Vrp+ cells was the same as that into Vrp- cells. The inclusion of 3H-Ara-C into S-phase cell DNA in the cells Vrp+ was 3 time that in the cells Vrp-. All the responders to cytosar treatment had Vrp- blasts. It is evident that evaluation of Vrp effect on 3H-Vcr accumulation under short-term culturing is able to indicate groups of patients with low probability of achieving complete remissions in response to standard regimens of Vcr, Ara-C and anthracyclines treatment.
Mukhammed
A
A
Turkina
A G
AG
Al'pidovskiĭ
V K
VK
Sidorova
T A
TA
Golenko
O D
OD
Gerasimova
G K
GK
D000293:Adolescent; D000328:Adult; D000368:Aged; D004351:Drug Resistance; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy*; D008875:Middle Aged; D010641:Phenotype; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy*; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014700:Verapamil
D014316:Tritium; D014700:Verapamil
Ispol'zovanie verapamila v testakh dlia opredeleniia lekarstvennoĭ ustoĭchivosti leĭkoznykh blastov.