Analysis of sites of foot and mouth disease virus persistence in carrier cattle via the polymerase chain reaction.
This study was undertaken in order to explore possible sites of foot-and-mouth disease virus (FMDV) persistence during the carrier state. Tissue samples taken from experimentally infected animals at different times post-infection (p.i.) were examined by conventional viral isolation and the polymerase chain reaction (PCR) technique. The analysis of samples from several organs taken from 17 bovines between 3 and 270 days p.i. allowed the following conclusions: 1) Virus present in oesophageal-pharyngeal fluids (OPF) during the carrier state originates in the pharynx as shown by the detection of antisense FMDV RNA by PCR, 2) PCR is more sensitive than standard virus isolation techniques and may be used for the rapid detection of FMDV in specimens obtained during the acute stage of FMD and for identification of persistently infected cattle.
Prato Murphy
M L
ML
Institute of Virology, CICV-INTA, Moron, Argentina.
Meyer
R F
RF
Mebus
C
C
Schudel
A A
AA
Rodriguez
M
M
D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000302:isolation & purification*; D015139:Blotting, Southern / Q000662:veterinary; D002353:Carrier State / Q000382:microbiology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D005536:Foot-and-Mouth Disease / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary
D004279:DNA, Viral