Suppressive effects of 2-acetylaminofluorene on concanavalin A-stimulated murine splenocyte proliferation in vitro: inhibition of interleukin-2 receptor expression.
Treatment of 2-acetylaminofluorene (AAF) to murine splenocyte culture produced a dose-related suppression on the lymphoproliferative response to concanavalin A (Con A). The amount of interleukin 2 (IL-2) activity in the culture supernatants was increased when AAF was treated for 48 hr. Since IL-2 activity did not increase if AAF was treated for the last 4 hr of a 48-hr culture period, the increase of IL-2 activity in culture supernatants did not appear to be due to the leakage of IL-2 from intracellular pool. Treatment of colchicine, an agent known to increase IL-2 activity in culture supernatants by inducing the cytoskeletal structure modification, increased IL-2 activity in splenocyte culture supernatants in 4 hr treatment. Meanwhile, the IL-2 receptor alpha (IL-2R alpha) positive cell population was decreased by the treatment of AAF. These results suggested that suppressive effects of AAF on the lymphoproliferative response to Con A in murine splenocyte culture may be associated with the inhibition of IL-2 receptor expression.
Koh
W S
WS
Department of Life Science, Korea Advanced Institute of Science and Technology, Taejon.
Chun
Y J
YJ
Yang
K H
KH
D015073:2-Acetylaminofluorene / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003208:Concanavalin A / Q000037:antagonists & inhibitors*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005455:Fluorescent Antibody Technique; D007376:Interleukin-2 / Q000378:metabolism; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000096:biosynthesis; D013154:Spleen / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D003208:Concanavalin A; D015073:2-Acetylaminofluorene; D003078:Colchicine