Vanadium inhibition of human parietal lobe ATPases.
In vitro influence of vanadate and vanadyl ions on the activities of Na,K- and Ca,Mg-ATPase from synaptosomal membranes of the parietal lobe of the human brain were compared. 1. Vanadate and vanadyl inhibit the enzymes activities in the investigated fraction. 2. Vanadate is a more effective inhibitor of both ATPases in the concentrations above 50 microM and vanadyl is an effective inhibitor in a very low concentration (10 nM). 3. Vanadate seems to be an uncompetitive inhibitor of Na,K-ATPase (K1 = 880 nM).
II Department of Biochemistry, Medical University of Lód, Poland.
G
G
Janiszewska
L
L
Lachowicz
D
D
Jaskólski
E
E
Gromadzińska
D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors*; D006801:Humans; D066298:In Vitro Techniques; D010296:Parietal Lobe / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013570:Synaptic Membranes / Q000201:enzymology; D009435:Synaptic Transmission / Q000502:physiology; D013574:Synaptosomes / Q000201:enzymology; D014638:Vanadates / Q000494:pharmacology; D014639:Vanadium / Q000494:pharmacology* / Q000502:physiology
D014639:Vanadium; D014638:Vanadates; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase