A homozygous deletion in a small cell lung cancer cell line involving a 3p21 region with a marked instability in yeast artificial chromosomes.
All types of lung carcinoma are characterized by a high frequency of loss of sequences from the short arm of chromosome 3, the smallest region of overlap containing D3F15S2 in band p21. Here we characterize a 440-kilobase segment from this region, which we found homozygously deleted in one of our small cell lung cancer-derived cell lines. The homozygous deletion maps between UBE1L and ZnF16, just centromeric to D3F15S2. Yeast artificial chromosomes with inserts originating from the deleted region are very unstable and readily lose parts of their insert.
Kok
K
K
Department of Medical Genetics, University of Groningen, The Netherlands.
van den Berg
A
A
Veldhuis
P M
PM
van der Veen
A Y
AY
Franke
M
M
Schoenmakers
E F
EF
Hulsbeek
M M
MM
van der Hout
A H
AH
de Leij
L
L
van de Ven
W
W
D001483:Base Sequence; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002872:Chromosome Deletion; D018244:Chromosomes, Artificial, Yeast; D002893:Chromosomes, Human, Pair 3; D006720:Homozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured