Contractile properties and temperature sensitivity of the extraocular muscles, the levator and superior rectus, of the rabbit.
1. Contractile and fatigue-resistance characteristics, temperature sensitivity (10-37 degrees C) of contraction, and histochemical fibre types were determined for two of the extraocular muscles, the superior rectus and levator palpebrae superioris (levator), of the rabbit. 2. The levator displayed similar contractile characteristics (time to peak, half-relaxation time of twitch response, and twitch-tetanus force ratio) to mammalian fast-twitch limb muscle at room temperature (20 degrees C). However, normalized twitch and tetanic force levels were significantly less than those found in limb muscle. The superior rectus displayed the characteristics of even faster contraction than the levator at 20 degrees C, but generated lower maximum force levels than the levator. 3. The twitch response of the superior rectus showed a biphasic relaxation phase. This response was not due to non-twitch (tonic) fibres present in the superior rectus as it was unaffected by propranolol application during muscle stimulation. 4. The superior rectus and levator displayed significantly less fatigue in the tetanic force response than fast-twitch limb muscles did in response to a fatiguing electrical stimulation protocol. The levator was significantly more fatigue resistant than the superior rectus. 5. The force responses of both extraocular muscles displayed a similar dependence on temperature (10-37 degrees C) to limb skeletal muscles. 6. The superior rectus and levator exhibited a high proportion of fast-twitch muscle fibres (type II) as shown by myosin ATPase staining. Succinate dehydrogenase activity indicated that these muscles showed a high oxidative capacity, with a staining intensity typical of type I or type II A fibres of limb muscles. 7. The results emphasize the morphological and functional complexity of mammalian extraocular muscles. The combination of very fast contractile properties with high oxidative capacity make these muscles well suited to their role in eye/eyelid movement.
Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.
B R
BR
Frueh
A
A
Hayes
G S
GS
Lynch
D A
DA
Williams
D000818:Animals; D004558:Electric Stimulation; D006651:Histocytochemistry; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000502:physiology; D009126:Muscle Relaxation / Q000502:physiology; D009218:Myosins / Q000378:metabolism; D009801:Oculomotor Muscles / Q000033:anatomy & histology / Q000201:enzymology / Q000502:physiology*; D010084:Oxidation-Reduction; D011817:Rabbits; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013696:Temperature
D013385:Succinate Dehydrogenase; D009218:Myosins