Active and passive protection against variant and classic infectious bursal disease virus strains induced by baculovirus-expressed structural proteins.
Infectious bursal disease virus (IBDV) is responsible for a highly immunosuppressive disease in young chickens which causes significant economic losses to the poultry industry worldwide. The structural protein genes (VP2, VP3 and VP4) of a variant IBDV strain (GLS) were expressed in insect cells using a baculovirus expression system. Susceptible chickens vaccinated with a single dose of the recombinant IBDV antigens were completely protected against challenge with two variant strains of IBDV (GLS and Delaware), and partially protected against the standard challenge strain (STC). A booster dose of the recombinant antigens induced higher levels of neutralizing antibodies and afforded complete protection against both variant and standard virus challenges. Specific-pathogen-free hens vaccinated with a single dose of the same subunit vaccine produced virus-neutralizing antibodies that were capable of passively protecting the progeny from infection with variant IBDV.
Vakharia
V N
VN
Center for Agricultural Biotechnology of Maryland Biotechnology Institute, University of Maryland, College Park 20742.
Snyder
D B
DB
Lütticken
D
D
Mengel-Whereat
S A
SA
Savage
P K
PK
Edwards
G H
GH
Goodwin
M A
MA
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D018175:Birnaviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D002060:Bursa of Fabricius / Q000276:immunology / Q000382:microbiology; D002645:Chickens / Q000276:immunology*; D005822:Genetic Vectors; D007116:Immunization, Passive / Q000662:veterinary*; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D009036:Moths; D009500:Neutralization Tests; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D011201:Poultry Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000276:immunology; D015678:Viral Structural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines