Hyperploidization of V79 cells by K-252a in comparison with demecolcine.
The cellular responses of the V79 cell cycle to K-252a, a protein kinase inhibitor, were examined by flow cytometry (FCM) and by time-lapse videomicrography. At a concentration of 0.5 microM of K-252a for 72 h, V79 cells became hyperploid, having above 32c DNA content. The cycle times in the hyperploidizing process were 11.2 (4c-8c), 16.3 (8c-16c) and 19.3 (16c-32c) hours, values which were less than twice that of the control (10.5 h). After the washout of K-252a, the DNA content was widely distributed in the V79 cell population and hyperploid V79 cells reduced DNA content through a variety of cell division modes. These cellular responses to hyperploidization of V79 cells by K-252a were in reasonable agreement with those by demecolcine.
Division of Basic Science, Kanazawa Medical University, Ishikawa, Japan.
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Fujikawa-Yamamoto
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Teraoka
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Odashima
D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects* / Q000235:genetics; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D004261:DNA Replication / Q000187:drug effects; D003703:Demecolcine / Q000494:pharmacology*; D026121:Indole Alkaloids; D008954:Models, Biological; D011003:Ploidies; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014407:Tumor Cells, Cultured
D002227:Carbazoles; D026121:Indole Alkaloids; C049985:staurosporine aglycone; D011493:Protein Kinase C; D003703:Demecolcine