[The determination of leukocyte osmotic resistance in hypertonic solutions of low-molecular nonelectrolytes].
A method for in vitro assessment of blood leukocyte osmotic stability has been developed. A characteristic feature of this method is assessment of qualitative and quantitative parameters in hypo- and hypertonic solutions of low-molecular nonelectrolytes characterized by erythrocytolytic properties (glycerol, urea, dimexide). The error is +/- 10%. The results of the method are compatible and reproducible, it gives sufficient volume of information about leukocyte osmotic stability as an integral indicator of these cells physicochemical properties. A parabolic type of osmotic stability relationship with concentrations in the 0.04 to 5.0 M range has been detected. The optimal concentrations at which the highest leukocyte intactness is observed are 2.0 M for glycerol, 0.5 M for urea, and 5.0 M for dimexide.
M P
MP
Vavilov
L A
LA
Apollonova
E K
EK
Nazarova
D000818:Animals; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D007552:Isotonic Solutions; D007962:Leukocytes / Q000187:drug effects* / Q000502:physiology; D008970:Molecular Weight; D009997:Osmotic Pressure / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D007552:Isotonic Solutions; D012965:Sodium Chloride; D014508:Urea; D005990:Glycerol; D004121:Dimethyl Sulfoxide
Opredelenie osmoticheskoĭ stoĭkosti leĭkotsitov v gipertonicheskikh rastvorakh nizkomolekuliarnykh neélektrolitov.