Contribution of membrane-associated cytotoxic proteins to cytolysis of L929 and K562 target cells mediated by LAK cells with different phenotypes.
We have demonstrated that the relative contribution of secreted and membrane-associated proteins to the cytotoxicity of LAK cells depended on LAK cell phenotype: the cytotoxicity of CD16+ CD8+ CD3- LAK cells was associated mainly with membrane-bound proteins, and the activity of CD3+ CD8+ CD16- LAK cells was due mainly to secreted soluble proteins. The cytotoxic activity of membrane fractions of LAK cells against cell line L929 was determined by 40-, 32- and 21-kDa proteins for LAK cells bearing NK-specific markers and due to proteins of 34 and 21 kDa in the case of 'CTL-like' LAK-cells.
Institute of Gene Biology, Russian Academy of Sciences, Moscow.
L P
LP
Saschenko
N V
NV
Gnuchev
I V
IV
Redchenko
O D
OD
Kabanova
T I
TI
Lukjianova
Blishchenko LYu
D K
DK
Satpaev
Chertov OYu
D000818:Animals; D003603:Cytotoxins / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D016130:Immunophenotyping; D015979:Killer Cells, Lymphokine-Activated / Q000502:physiology*; D007739:L Cells / Q000276:immunology; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D014407:Tumor Cells, Cultured / Q000276:immunology
D003603:Cytotoxins; D008565:Membrane Proteins