Susceptibility of monoclonal IgG paraproteins to plasmic cleavage using glycerin-stabilized human plasmin.
To shed further light on plasmin-IgG interactions a simple procedure is described that permitted 48 monoclonal IgG isolates from human serum to be profiled for their susceptibility to plasmic cleavage. In addition to anodal Fc and cathodal Fab fragments, combined immunoelectrophoresis-electrophoresis revealed transient anodal banding, as well as Fab-fragment subcleavage in many of the IgG subclass-1 isolates. The subcleavage of released Fab fragments, which bear the idiotype determinants, points to a possible ancillary role of plasmin in "idiotype processing" leading to immunoregulatory anti-idiotype networks. The cleavage of IgG by endogenous plasmin also points to a possible active role of plasmin in the "steady-state" metabolism of IgG.
Department of Pathology, New York University Medical Center, NY 10016.
J V
JV
Chuba
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D005341:Fibrinolysin / Q000378:metabolism*; D005990:Glycerol; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D010266:Paraproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D017433:Protein Structure, Secondary; D011817:Rabbits; D051381:Rats; D013379:Substrate Specificity
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D010266:Paraproteins; D005341:Fibrinolysin; D005990:Glycerol