Various factors influencing the signal intensity of oligonucleotides in electrospray mass spectrometry.
The quality of electrospray mass spectra of oligonucleotides often suffers from the low signal intensity and clustering with metal ions. This sometimes makes an exact determination of the molecular masses impossible. We describe here several factors contributing to these effects and show some means for their improvement.
Institut für Organische Chemie, Universität Tübingen, Germany.
K
K
Bleicher
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E
Bayer
D001483:Base Sequence; D004247:DNA / Q000032:analysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D009841:Oligonucleotides / Q000032:analysis*
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA