Determination of clonidine in human plasma by gas chromatography-electron-impact mass spectrometry.
A new and sensitive gas chromatographic-mass spectrometric (GC-MS) assay for routine analysis of clonidine in human plasma is described. Quantification is carried out with simple electron-impact ionization mass spectrometry and solid-phase cartridges for preliminary extraction from plasma. The pentafluorobenzyl derivative of clonidine yields an intense ion fragment at m/z 354, and the lower limit of detection is 0.025 ng/ml for a 1-ml plasma sample. The practical applicability of this method is demonstrated by determining plasma concentrations of clonidine in a clinical study of a new transdermal delivery system for clonidine.
Yamahata
T
T
Research Laboratories, Maruho Co., Ltd., Osaka, Japan.
Dote
S
S
Ozawa
Y
Y
Nishikawa
H
H
Maeda
S
S
D000279:Administration, Cutaneous; D003000:Clonidine / Q000008:administration & dosage / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans
D003000:Clonidine