Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody specific for the S antigen of hepatitis B virus.
The nucleotide (nt) sequences encoding the variable regions of the heavy and light chains of a murine monoclonal antibody (mAb) have been determined. The mAb recognizes a disulfide-bond-dependent conformational epitope on the hepatitis B virus surface antigen. The sequence analyses revealed that the variable regions of the heavy and light chains are members of mouse heavy-chain subgroup II(A) and kappa light-chain subgroup III, respectively.
Ryu
C J
CJ
Genetic Engineering Research Institute, KIST, Taejon, South Korea.
Jin
B R
BR
Chung
H K
HK
Han
M H
MH
Hong
H J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D018076:DNA, Complementary; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D006514:Hepatitis B Surface Antigens; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region