Effect of three Japanese kampo medicines on platelet activation by monoclonal anti-platelet membrane glycoprotein antibodies.
We studied the effect of three Japanese kampo medicines on platelet activation by an anti-CD9 monoclonal antibody (NNKY1-19) and an anti-human Fc gamma receptor II monoclonal antibody (NNKY3-2). Sho-saiko-to (TJ-9) and Sairei-to (TJ-114) partially suppressed platelet aggregation induced by NNKY1-19, while Juzen-taiho-to (TJ-48) suppressed aggregation induced by NNKY3-2. TJ-9 and TJ-114 also suppressed collagen-induced aggregation, but TJ-48 did not. Flow cytometry showed that the three medicines did not affect antibody binding to the platelets. Thus, all three kampo medicines suppressed platelet activation by anti-platelet glycoprotein antibodies without inhibiting antibody binding.
First Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
T
T
Kawakatsu
S
S
Nomura
H
H
Kido
K
K
Yamaguchi
T
T
Fukuroi
M
M
Suzuki
M
M
Yanabu
T
T
Kokawa
K
K
Yasunaga
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D016824:Antigens, Human Platelet / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007155:Immunologic Factors / Q000494:pharmacology; D066298:In Vitro Techniques; D015539:Platelet Activation / Q000187:drug effects*; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D004365:Drugs, Chinese Herbal; D007141:Immunoglobulin Fc Fragments; D007155:Immunologic Factors; D010980:Platelet Membrane Glycoproteins; C063587:juzentaihoto; C080903:sairei-to; C018142:saiko-keishi-to