Electron holographic observation of thin biological filaments.
A non-staining bacterial flagellum filament only 24 nm in diameter has been observed clearly by using a phase-shifting electron holographic technique. This method produces a sufficiently high contrast in-focus image that could never be obtained by ordinary transmission electron microscopic (TEM) methods.
Tonomura Electron Wavefront Project, ERATO, JRDC, Saitama, Japan.
K
K
Aoyama
G
G
Lai
Q
Q
Ru
D005407:Flagella / Q000648:ultrastructure*; D006696:Holography / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D012486:Salmonella typhimurium / Q000648:ultrastructure*