Purification of 14-3-3 protein and analysis of isoforms in chicken brain.
14-3-3 proteins are apparently ubiquitous eukaryotic proteins that comprise a large number of isoforms. We have used specific antibodies raised against each mammalian isoform to probe for 14-3-3 isoforms in adult hen brains. The results suggest that there is a remarkable degree of similarity in primary structure (at least in the regions containing the epitopes). Reverse-phase HPLC of the purified avian 14-3-3 proteins indicates a high overall degree of similarity in sequence and levels of expression of each isoform that are remarkably similar to their mammalian counterparts.
Laboratory of Protein Structure, National Institute for Medical Research, Mill Hill, London, UK.
Y
Y
Patel
H
H
Martin
S
S
Howell
D
D
Jones
K
K
Robinson
A
A
Aitken
D048948:14-3-3 Proteins; D000818:Animals; D001923:Brain Chemistry; D002645:Chickens; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D014446:Tyrosine 3-Monooxygenase