Determination of porphyrins in bile using high performance liquid chromatography and some clinical applications.
A simple and fast HPLC method for the determination of porphyrins in bile without extraction is described. Porphyrins were determined in bile from control subjects and from patients after orthotopic liver transplantation, including three patients with erythropoietic protoporphyria. It was found that: 1) coproporphyrin I is the predominant porphyrin in bile of controls, accompanied by some coproporphyrin III and protoporphyrin, whereas protoporphyrin mostly but not always is the predominant porphyrin in the bile of erythropoietic protoporphyria patients. In two of the three erythropoietic protoporphyria patients, the bile contained a hundred times more protoporphyrin than that of non-porphyric orthotopic liver transplantation patients. The third erythropoietic protoporphyria patient remained cholestatic and was unable to excrete sufficient amounts of protoporphyrin. 2) All investigated bile samples contained no secondary porphyrins derived from protoporphyrin, i.e. no deutero-, pempto-, or mesoporphyrin. Even when extracts of bile and serum were concentrated fifty to a hundred times, no traces of deutero-, pempto- and mesoporphyrin were detected. This complete absence of secondary porphyrins suggests that an enterohepatic circulation of dicarboxylic porphyrins from the distal gastrointestinal tract does not exist. 3) The HPLC chromatograms contain peaks from unknown compounds. No correlation between porphyrins and these compounds was found. Porphyrin profiles were followed in the bile of some orthotopic liver transplantation patients. Three episodes are recognizable. During the first three days after orthotopic liver transplantation there is a very high coproporphyrin excretion. There is then a lag of one to three weeks, in which no or very low porphyrin concentrations are detectable, followed by the restoration of normal biliary porphyrin patterns.
Department of Clinical Chemistry, University Hospital, Groningen, The Netherlands.
G J
GJ
Beukeveld
L
L
Meerman
J R
JR
Huizenga
E E
EE
Venekamp-Hoolsema
C H
CH
Gips
B G
BG
Wolthers
D001646:Bile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D016031:Liver Transplantation; D017121:Porphyria, Hepatoerythropoietic / Q000378:metabolism / Q000601:surgery; D011166:Porphyrins / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results
D011166:Porphyrins