Detection of cochlear dysfunction by the measurement of transiently evoked otoacoustic emissions in guinea pigs with autoimmune-induced labyrinthitis.
For the evaluation of functional inner ear lesions caused by an autoimmune-induced labyrinthitis, transiently evoked otoacoustic emissions (TEOAEs) were detected in guinea pigs before and after transfer of sensitized lymphocytes from animals suffering from a labyrinthitis induced by the foreign protein keyhole limpet hemocyanin (KLH). Initially TEOAEs were detectable from 47 of 62 ears (76%) in healthy guinea pigs. These animals then were used as recipients for sensitized lymphocytes from donors exclusively. Three months after cell transfer the incidence of TEOAEs was reduced to 48% in animals receiving lymphocytes from donors sensitized with KLH intradermally and intracochlearly. In recipients of lymphocytes from donors sensitized only intradermally and in untreated animals no significant alteration of the TEOAE incidence was found. These findings showed strong correlation with those from scanning electron microscopy of the organ of Corti, indicating that the measurement of TEOAE is a useful, time-saving tool for the detection of cochlear dysfunction caused by sensitized lymphocytes in experimental animal. The present findings also show that the migration of sensitized lymphocytes actually leads to functional lesions in the cochlea.
Ross
U H
UH
Department of Oto-Rhino-Laryngology, University of Freiburg, Germany.
Rogowski
M
M
Reiss
G
G
Gloddek
B
B
D000818:Animals; D001307:Auditory Perception / Q000502:physiology*; D001327:Autoimmune Diseases / Q000473:pathology / Q000503:physiopathology*; D003051:Cochlea / Q000503:physiopathology* / Q000648:ultrastructure; D005072:Evoked Potentials, Auditory / Q000502:physiology*; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006168:Guinea Pigs; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology; D007114:Immunization; D007267:Injections; D007271:Injections, Intradermal; D007762:Labyrinthitis / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology*; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology; D008855:Microscopy, Electron, Scanning; D009925:Organ of Corti / Q000648:ultrastructure; D012022:Reflex, Acoustic / Q000502:physiology; D012533:Scala Tympani
D005620:Freund's Adjuvant; D006433:Hemocyanins