Evaluation of an orally administered vaccine, using hydrogels containing bacterial exotoxins of Pasteurella haemolytica, in cattle.
Poly(methacrylic acid) hydrogels were tested for oral delivery of a vaccine against Pasteurella haemolytica infection in cattle. Culture supernatants of P haemolytica, the most common bacterium associated with pneumonia in cattle, were used as the antigens in the vaccine. Hydrogels containing culture supernatants were administered orally to calves. Calves were then challenge-exposed with virulent P haemolytica. Calves were euthanatized 3 days after challenge exposure. The lungs of each calf were scored for severity and size of pneumonic lesions. Results indicated that vaccinated calves had smaller, less severe pneumonic lesions and lived longer than nonvaccinated calves. These results indicated that hydrogels can be used to deliver vaccines orally to calves to enhance resistance to pneumonia caused by P haemolytica.
School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907-1243.
T L
TL
Bowersock
W S
WS
Shalaby
M
M
Levy
M L
ML
Samuels
R
R
Lallone
M R
MR
White
D L
DL
Borie
J
J
Lehmeyer
K
K
Park
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D015151:Immunoblotting; D016978:Mannheimia haemolytica / Q000276:immunology*; D010326:Pasteurella Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D011092:Polyethylene Glycols
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D017077:Culture Media, Conditioned; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols