Simple and rapid determination of phytase activity.
A simple and rapid method is described for determining the enzymatic activity of microbial phytase. The method is based on the determination of inorganic orthophosphate released on hydrolysis of sodium phytate at pH 5.5.
Engelen
A J
AJ
Gist-brocades b.v., Intracompany Service Laboratory, Delft, The Netherlands.
van der Heeft
F C
FC
Randsdorp
P H
PH
Smit
E L
EL
D010832:6-Phytase / Q000032:analysis* / Q000378:metabolism; D001234:Aspergillus niger / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000032:analysis / Q000378:metabolism; D010833:Phytic Acid / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry
D010710:Phosphates; D010833:Phytic Acid; D010832:6-Phytase