Simultaneous separation and quantification of free and metal-chelated protoporphyrins in blood by three-dimensional HPLC.
In this new method for simultaneous separation and quantification of free and metal-chelated porphyrins in blood, the porphyrins are extracted from blood samples with a mixture of diisopropylamine:water:methanol (25:100:900, by vol) and separated by HPLC elution. The data are collected in three-dimensional form with a microcomputer. This method permits a high recovery of protoporphyrin (PP) and Zn-chelated PP as well as heme from blood. By this method, the amounts of these analytes in erythrocytes from normal or abnormal subjects can be determined more accurately than by conventional methods.
Sato Pharmaceutical Research Institute, Inc., Chiba-ken, Japan.
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Sato
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Ido
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Kimura
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry; D006418:Heme / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007700:Kinetics; D007855:Lead Poisoning / Q000097:blood; D008297:Male; D000432:Methanol; D011437:Propylamines; D011524:Protoporphyrins / Q000097:blood*; D012997:Solvents; D013050:Spectrometry, Fluorescence; D014867:Water
D006454:Hemoglobins; D011437:Propylamines; D011524:Protoporphyrins; D012997:Solvents; D014867:Water; C017803:zinc protoporphyrin; D006418:Heme; C007442:diisopropylamine; D000432:Methanol