Chemiluminescent hybridization assay for hepatitis delta virus RNA in serum.
A nonradioactive hybridization assay for the detection of hepatitis delta virus RNA in serum is described. This assay utilizes a digoxigenin-labeled RNA hybridization probe and chemiluminescent immunodetection. The probe can detect as little as 0.4 pg of cDNA with a 60-min exposure. Results obtained are in agreement with serological indicators of hepatitis delta virus infection and are comparable to those obtained by hybridization assays employing radioactively labeled RNA.
Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
M
M
Gallagher
T T
TT
Morris
H A
HA
Fields
D005069:Evaluation Studies as Topic; D003699:Hepatitis D / Q000175:diagnosis* / Q000382:microbiology; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
D015347:RNA Probes; D012367:RNA, Viral