Immune response to Moraxella catarrhalis in children with otitis media: opsonophagocytosis with antigen-coated latex beads.
Opsonic antibody activity against Moraxella catarrhalis was determined in sera from children with otitis media. The antibody was determined with a new assay utilizing outer membrane antigen-coated latex beads. Antigen-coated beads opsonized in heat-inactivated pooled human serum phagocytosed 47.5 +/- 36.1 beads per 100 neutrophils compared to 15.6 +/- 10.2 beads per 100 neutrophils opsonized in hypogammaglobulinemic serum (p < .025). Antigen-coated beads opsonized in homologous sera from 11 children with M. catarrhalis otitis media demonstrated increased opsonic activity in convalescent sera (34.6 +/- 27.1) compared to acute sera (15.5 +/- 6.7; p < .05). These data suggest that infection with M catarrhalis is associated with the development of opsonic antibody.
Department of Pediatrics, State University of New York, Buffalo School of Medicine.
H
H
Faden
J J
JJ
Hong
N
N
Pahade
D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002648:Child; D006801:Humans; D007159:Immunologic Tests; D007840:Latex; D008863:Microspheres; D001936:Moraxella catarrhalis / Q000276:immunology* / Q000302:isolation & purification; D016870:Neisseriaceae Infections / Q000276:immunology; D009895:Opsonin Proteins / Q000276:immunology*; D010033:Otitis Media / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007840:Latex; D009895:Opsonin Proteins