[Effect of prolonged hemodialysis therapy on activity of superoxide dismutase and glutathione peroxidase in erythrocytes and leukocytes of patients with chronic renal failure].
The questions to be answered were: 1) what was the enzymatic activity of free radicals removal in patients with chronic renal failure treated with haemodialysis (CRF) and 2) what was the long-term effect of haemodialysis therapy activity of those enzymes. 51 patients with chronic renal failure treated with hemodialysis (CRF) and healthy subjects (control) were observed: in 29 patients treated with haemodialysis (HD) less than 4 years (group A); 11 patients treated with HD 4-8 years (group B); 11 patients treated with HD over 8 years (group C). Immediately before and after HD (cuprophan dialyser) blood samples were drawn and activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in erythrocytes and leukocytes was measured. In healthy subjects only one estimation was made. SOD activity was measured with the Misra-Fridovich method and GPX activity in erythrocytes and leukocytes was significantly lower in uremic subjects. A significant increase of SOD activity in erythrocytes was seen in long-term HD patients. A significant positive correlation between erythrocytes SOD activity and time of HD therapy was present. During HD with cuprophan dialyser a significant decrease activity above mentioned hormones was observed. In uremic subjects treated with HD enzymatic activity of autooxidans was significantly lower than in normals. Long-term HD therapy did not influence the activity of those enzymes. Only erythrocyte SOD activity significantly rose in long-term HD patients. After haemodialysis with cuprophan dialyser a significant decrease SOD and GPX activity was observed.
Katedry i Kliniki Chorób Wewnetrznych, Zabrzu.
W
W
Grzeszczak
B
B
Galwas
A
A
Jendryczko
J
J
Pachelski
E
E
Zukowska-Szczechowska
D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000201:enzymology* / Q000628:therapy*; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D013482:Superoxide Dismutase / Q000097:blood
D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
Wpływ wieloletniej hemodializoterapii na aktywność dysmutazy ponadtlenkowej i peroksydazy glutationu w erytrocytach i leukocytach u chorych z przewlekła niewydolnościa nerek.