Establishment and characterization of synovial cell clones with integrated human T-cell leukemia virus type-I.
Human T cell leukemia virus type-I (HTLV-I)-integrated synovial cell clones (SCCs) were established from four HTLV-I-infected patients with chronic proliferative arthropathy. Extracted DNAs of these SCCs were examined for the existence of HTLV-I proviral DNA. Electron microscopic analysis and immunohistochemical staining were also performed to examine the nature of the HTLV-I integrated SCCs. SCCs derived from all four patients consisted of either HTLV-I integrated or nonintegrated cells. HTLV-I integrated SCCs also produced viral messenger RNA which was detected by reverse transcriptase-polymerase chain reaction as well as viral protein which was detected by immunohistochemical staining. Phenotypically, these SCCs containing HTLV-I proviral DNA were shown to be all cell types as observed by electron microscopic analysis and immunohistochemical analysis. Moreover, expression of HTLV-I glycoprotein was greater in SCCs with a monocyte marker. These findings suggest that SCCs harboring HTLV-I have an important role in synovial hyperplasia.
Hasunuma
T
T
Division of Rheumatology and Molecular Immunology, St. Marianna University School of Medicine, Kanagawa-ken, Japan.
Nakajima
T
T
Aono
H
H
Sato
K
K
Matsubara
T
T
Yamamoto
K
K
Nishioka
K
K
D000368:Aged; D000369:Aged, 80 and over; D002999:Clone Cells; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D013583:Synovial Membrane / Q000166:cytology* / Q000382:microbiology*
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral