Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR.
Analysis of hepatitis C virus (HCV) replication has been hampered due to the difficulty encountered in in vitro cultivation of the virus in conventional tissue culture systems. In this study, primary chimpanzee hepatocyte cultures maintained in a serum-free medium formulation were susceptible to in vitro infection with HCV. In order to document infection, two new methods of reverse transcription/polymerase chain reaction were developed that permit accurate distinction between positive and negative strand HCV RNA. One method relied upon the use of a tagged cDNA primer, while the second method employed a thermostable reverse transcriptase. Following inoculation of chimpanzee hepatocytes with HCV, intracellular positive and negative strand HCV RNA were detectable 4 days postinfection and throughout the remainder of the experimental period, 25 days. Analysis of HCV-inoculated baboon hepatocytes revealed a total absence of negative strand HCV RNA, while residual positive strand RNA from the inoculum could be detected for up to 11 days. The in vitro replication of HCV RNA in chimpanzee hepatocytes could be suppressed by alpha-interferon. This system should be amenable to the study of HCV replication, antiviral compounds, and the development of neutralization assays.
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228.
R E
RE
Lanford
C
C
Sureau
J R
JR
Jacob
R
R
White
T R
TR
Fuerst
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D016174:Hepacivirus / Q000254:growth & development*; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000494:pharmacology; D008099:Liver / Q000382:microbiology*; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000382:microbiology*; D010215:Papio; D012367:RNA, Viral / Q000032:analysis*; D013045:Species Specificity; D014779:Virus Replication / Q000187:drug effects
D017931:DNA Primers; D016898:Interferon-alpha; D012367:RNA, Viral