Multiple NF-I-site binding factors associated with different cell-types of human lung carcinomas.
Nuclear extracts from human lung adenocarcinoma and squamous carcinoma cells were found to form two types of DNA-protein complexes with a consensus nuclear factor I (NF-I)-binding site. Three NF-I-site-binding cofactors (NCF90, NCFIIO and NCF120) were found to be differentially expressed in these two cell-types of human lung carcinomas. These data suggest that the NF-I-binding enhancer element with its differentially expressed NF-I cofactors may play an important role in the differentiation of neoplastic human bronchial epithelium.
Department of Medicine, San Fernando Valley Program, University of California, Los Angeles.
Y C
YC
Wong
S D
SD
Bernal
D000230:Adenocarcinoma / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D018287:Carcinoma, Large Cell / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D016384:Consensus Sequence; D004273:DNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human