Multiplicity of dog kidney high-Km aldose reductase and conversion mechanism into aldose reductase.
1. High-Km aldose reductase purified from dog kidney inner medulla was easily converted into aldose reductase by incubation in the neutral buffer solution. 2. High-Km aldose reductase was found to be in multiple forms, and was separated into three kinds of species designated as a-, b- and c-forms by HPLC. 3. The a-form observed as a single peak by HPLC was assumed to be present in three forms (a1-, a2- and a3-forms), one was aldose reductase (a1-form) and the others were the precursors of aldose reductase (a2- and a3-form). 4. The b-form was rapidly converted into the a3-form, followed slowly by the a2-form and finally into the a1-form. 5. The c-form was either directly converted into the a1-form, or indirectly into the a2-form followed by the a1-form. 6. Four kinds of species (a2-, a3-, b- and c-forms) of high-Km aldose reductase were finally converted into aldose reductase (a1-form).
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan.
M
M
Ohta
T
T
Tanimoto
T
T
Hayakawa
D000449:Aldehyde Reductase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004789:Enzyme Activation; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000201:enzymology*; D007700:Kinetics; D008970:Molecular Weight
D000449:Aldehyde Reductase