Pediocin A, a bacteriocin produced by Pediococcus pentosaceus FBB61.
Pediocin A, a bacteriocin produced by Pediococcus pentosaceus FBB61, was purified and partially characterized. The purification was achieved by dialysis against PEG 20,000, butanol extraction and electroendosmotic preparative electrophoresis. The protocol led to a 7843-fold increase in the specific activity, with 3.9% activity recovered. SDS-PAGE of pediocin A resulted in a single 80 kDa protein band. The antimicrobial compound was sensitive to proteolytic enzymes and heat (10 min at 100 degrees C). It exhibited inhibition against species of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Staphylococcus, Enterococcus, Listeria and Clostridium.
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Istituto di Zootecnia e Nutrizione Animale, Facoltà di Medicina Veterinaria, Università di Bologna, Ozzano Emilia (BO), Italy.
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D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006358:Hot Temperature; D008970:Molecular Weight; D010376:Pediococcus / Q000737:chemistry*
D001430:Bacteriocins