The formation of reactive oxygen species in a fraction of rat brain by metabolism of nitric oxide.
The potential of nitric oxide for the induction of reactive oxygen species (ROS) has been evaluated in a cerebral synaptosomal fraction. Nitric oxide (NO) was generated using S-nitroso-N-acetylpenicillamine (SNAP). NO stimulated ROS production, assayed using a fluorescent probe. This stimulation was largely inhibited by superoxide dismutase but not by catalase, implying that superoxide anion but not hydrogen peroxide, was a critical intermediate. An iron chelator, deferoxamine or hydroxyl radical scavengers failed to prevent stimulation of ROS by SNAP. This suggests the possibility of formation of a novel class of short-lived active oxygen species by NO.
Department of Community and Environmental Medicine, University of California at Irvine 92717.
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D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D003676:Deferoxamine / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D010396:Penicillamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D026423:S-Nitroso-N-Acetylpenicillamine; D013482:Superoxide Dismutase / Q000494:pharmacology; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014665:Vasodilator Agents / Q000494:pharmacology*
D017382:Reactive Oxygen Species; D014665:Vasodilator Agents; D009599:Nitroprusside; D009569:Nitric Oxide; D000431:Ethanol; D008353:Mannitol; D026423:S-Nitroso-N-Acetylpenicillamine; D002374:Catalase; D013482:Superoxide Dismutase; D010396:Penicillamine; D003676:Deferoxamine