[The induction of specific immunity to human ovarian carcinoma cells by anti-idiotypic antibodies].
Monoclonal anti-idiotypic antibodies, 6B11 and H12, were employed instead of the tumor antigen to induce a specific cellular immunity to ovarian carcinoma cell line SKOV3. 6B11 and 1H12 carring the internal image of ovarian carcinoma antigen. BALB/c mice were immunized with 6B11 or 1H12 and then the mouse foot pad was attacked by the injection of target cell SKOV3 to stimulate a specific delayed-type hypersensitivity (DTH). The results showed that 6B11 induced a severe foot pad swelling (Mean thickness 0.93mm) as seen in the positive control (P > 0.05) induced by ovarian carcinoma antigen; the 1H12 induced swelling was significantly lower than the positive control (P < 0.05). Both 6B11 and 1H12 expressed the effective responses only to SKOV3, but not to the unrelated target cells. Pathological examinations found that there was marked infiltration of inflammatory cells in foot pad of mice primed by 6B11, while by 1H12, only induced a narrow pathological changes. These observations indicated that there would be some essential differences between 6B11 and 1H12 in the behavior of inducing the specific DTH to SKOK3. And as an idiotypic vaccine, 6B11 would be more powerful against ovarian carcinoma.
Research Laboratory of Gynecologic Oncology, People's Hospital, Beijing Medical University.
W Y
WY
Lu
H N
HN
Qian
W J
WJ
Li
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm / Q000276:immunology; D005260:Female; D006968:Hypersensitivity, Delayed / Q000209:etiology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010051:Ovarian Neoplasms / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm