Effect of chronic ethanol feeding on plasma and liver alpha- and gamma-tocopherol levels in normal and vitamin E-deficient rats. Relationship to lipid peroxidation.
The effects of chronic ethanol intake on the levels of alpha-tocopherol and gamma-tocopherol in serum and liver of both vitamin E-deficient and normal rats were studied. An intragastric feeding rat model was used. Both normal and vitamin E-deficient animals were fed a liquid diet and ethanol for 1 month. In pair-fed animals, dextrose was isocalorically replaced by ethanol. The blood ethanol level in the ethanol-fed animals was between 150 and 250 mg/dL. Liver peroxidation was determined by measuring thiobarbituric acid reactive substances (TBARS). Plasma alanine aminotransferase (ALT) was increased by 3-fold in vitamin E-deficient ethanol-fed rats compared with normal ethanol-fed rats. Plasma alpha- and gamma-tocopherol were decreased in the normal ethanol-fed rats by 22.3 and 65%, respectively (P < 0.01). Liver alpha- and gamma-tocopherol were also decreased by 51.7 and 76%, respectively (P < 0.01). Vitamin E-deficient animals had significantly lower mean plasma alpha-tocopherol (5670 vs 530 ng/mL, P < 0.01), and ethanol feeding did not decrease the levels any further. However, ethanol feeding decreased liver alpha- and gamma-tocopherol by 58.5 and 56.5% (P < 0.01), respectively, beyond the already low levels observed in this group. There was an inverse correlation between liver TBARS and liver alpha-tocopherol (r = -0.59, P < 0.05) and gamma-tocopherol (r = -0.65, P < 0.02). Also of significance is that ethanol feeding decreased the plasma and liver gamma-tocopherol more than the alpha-tocopherol in both normal and vitamin E-deficient animals. In conclusion, ethanol feeding markedly decreased both alpha- and gamma-tocopherol in livers of normal and vitamin E-deficient rats, but it only decreased plasma levels of tocopherols in normal rats. The higher ALT in vitamin E-deficient animals and the inverse correlation between TBARS and alpha- and gamma-tocopherol suggest that enhanced lipid peroxidation is associated with greater severity of liver injury induced by ethanol in vitamin E-deficient rats.
Department of Pathology, New England Deaconess Hospital, Boston, MA 02215.
S M
SM
Sadrzadeh
A A
AA
Nanji
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M
Meydani
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000431:Ethanol / Q000008:administration & dosage* / Q000097:blood / Q000494:pharmacology; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014810:Vitamin E / Q000097:blood / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000378:metabolism*
D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D000431:Ethanol; D000410:Alanine Transaminase