Inhibition of Abelson oncogene function by erbstatin analogues.
The authors examined the effect of a tyrosine kinase inhibitor, erbstatin, and its analogues on abl oncogene functions. Erbstatin and its stable analogue methyl 2,5-dihydroxycinnamate (2,5-MeC) inhibited the growth of v-ablts-NIH3T3 cells at the permissive temperature (33 degrees C) at lower concentrations than at the non-permissive temperature (39 degrees C). 2,5-MeC inhibited the morphological transformation and the activation of v-abl tyrosine kinase by the temperature shift (39 degrees C to 33 degrees C) more effectively than erbstatin. Previously the authors reported that erbstatin induced erythroid differentiation of K562 human chronic myelogenous leukaemia cells, so they examined the effect of erbstatin analogues on the erythroid differentiation. Among eight erbstatin analogues studied, ethyl 2,5-dihydroxycinnamate induced erythroid differentiation of K562 cells most effectively. Ethyl 2,5-dihydroxycinnamate also inhibited bcr-abl tyrosine kinase. These results indicate that the stable analogues of erbstatin suppress oncogene functions of Abl by inhibiting its tyrosine kinase.
Kawada
M
M
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Tawara
J
J
Tsuji
T
T
Honma
Y
Y
Hozumi
M
M
Wang
J Y
JY
Umezawa
K
K
D000011:Abelson murine leukemia virus / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D002472:Cell Transformation, Viral / Q000187:drug effects; D002934:Cinnamates / Q000494:pharmacology*; D016313:Genes, abl / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D006873:Hydroquinones / Q000494:pharmacology*; D016374:Oncogene Proteins v-abl / Q000037:antagonists & inhibitors* / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*
D002934:Cinnamates; D006873:Hydroquinones; D016374:Oncogene Proteins v-abl; C087931:ethyl 2,5-dihydroxycinnamate; C067472:methyl 2,5-dihydroxycinnamate; D011505:Protein-Tyrosine Kinases; C048212:erbstatin