Two-dimensional gas chromatography for the enantiomeric separation of ephedrine and phenaminum and their metabolites.
Two-dimensional gas chromatography was used for enantiomeric separation of ephedrine, phenaminum and their metabolites in human urine. The main column was coated with a 2,6-di-O-pentyl-3-O-trifluoroacetylated-alpha-cyclodextrin and an SE-54 phase column was used as precolumn. The results showed that two-dimensional chromatography could greatly simplify the sample preparation, eliminate the interference of sample matrices, and improve the resolution of the enantiomers of interest.
Wang
X
X
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences.
Jia
C
C
Wan
H
H
D000661:Amphetamine / Q000302:isolation & purification* / Q000652:urine; D002849:Chromatography, Gas; D003505:Cyclodextrins; D003913:Dextroamphetamine / Q000302:isolation & purification / Q000652:urine; D004809:Ephedrine / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D010665:Phenylpropanolamine / Q000302:isolation & purification / Q000652:urine; D013237:Stereoisomerism; D013607:Tablets; D047391:alpha-Cyclodextrins
D003505:Cyclodextrins; D013607:Tablets; D047391:alpha-Cyclodextrins; D010665:Phenylpropanolamine; D000661:Amphetamine; D004809:Ephedrine; D003913:Dextroamphetamine; C032613:alpha-cyclodextrin