Isoform patterns of lysosomal glycosidases in phenotypically different leukemic lymphoid cells.
Isoform patterns of six lysosomal glycosidases were studied in leukemic lymphoid cells phenotypically related to B and T cells at distinct stages of differentiation. In all types of cells, the activity of glycosidases under study was expressed in two major isoforms. No correlation was observed between isoform patterns and cell phenotypes. The beta-hexosaminidase isoform ratios for phenotypically related leukemic lymphoid cells but isolated from different sources (blood and spleen) differed. It was suggested that cell localization affects isoform expression. An anomalous alpha-mannosidase was detected in lymphoid cells from lymph nodes, while it was lacking in the phenotypically related blood lymphoid cells from the same patients. Isoform I of beta-hexosaminidase was recorded in lymphoid cells of patients with anemias.
Ushakova
N A
NA
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow.
Samojlova
R S
RS
Popova
I A
IA
Presnova
V N
VN
Preobrazhenskaya
M E
ME
D000740:Anemia / Q000201:enzymology*; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D005966:Glucuronidase / Q000032:analysis; D006026:Glycoside Hydrolases / Q000032:analysis*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007938:Leukemia / Q000201:enzymology*; D008198:Lymph Nodes / Q000201:enzymology; D008221:Lymphoid Tissue / Q000201:enzymology*; D008223:Lymphoma / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008361:Mannosidases / Q000032:analysis; D010641:Phenotype; D012751:Sezary Syndrome / Q000201:enzymology; D013154:Spleen / Q000201:enzymology; D013601:T-Lymphocytes / Q000276:immunology*; D043323:alpha-Mannosidase; D001619:beta-N-Acetylhexosaminidases / Q000032:analysis
D015703:Antigens, CD; D007527:Isoenzymes; D006026:Glycoside Hydrolases; D008361:Mannosidases; D043323:alpha-Mannosidase; D005966:Glucuronidase; D001619:beta-N-Acetylhexosaminidases