A gD homologous gene of feline herpesvirus type 1 encodes a hemagglutinin (gp60).
A gene-encoding glycoprotein D (gD) homologue of feline herpesvirus type 1 (FHV-1) was identified and sequenced. It was located within an EcoRI 4.3-kbp fragment in the middle of the unique short region. The primary translation product of the gD homologous gene is predicted to consist of 374 amino acids with a molecular weight (MW) of 43.2 kDa. Comparative analysis among gD counterparts of other herpesviruses revealed that six cysteine residues which correlate with disulfide bond structure were conserved, and the amino acid sequences had homologies of 32.6% with bovine herpesvirus type 1 gIV, 29.1% with pseudorabies virus gp50, 28.3% with equine herpesvirus type 1 gD, and 22.0% with herpes simplex virus type 1 gD. When the gD homologue was expressed in COS cells and examined by indirect immunofluorescence and immunoprecipitation assays, it was recognized only by monoclonal antibodies (MAbs) reactive with gp60 which have virus-neutralizing and hemagglutination (HA)-inhibition (HI) activities. This expressed product had a MW of approximately 60 kDa and was shown to be a hemagglutinin by HA and HI tests with the MAbs. These results suggest that the gD homologue of FHV-1 is a hemagglutinin.
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
K
K
Maeda
Y
Y
Kawaguchi
M
M
Ono
Y
Y
Inoshima
T
T
Miyazawa
Y
Y
Tohya
C
C
Kai
T
T
Mikami
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats / Q000382:microbiology; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics*; D006564:Herpesviridae / Q000235:genetics* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014759:Viral Envelope Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; C488988:glycoprotein D, Equid herpesvirus 1; C488986:glycoprotein D, Feline herpesvirus 1