Presence of dityrosine bridges in thyroglobulin and their relationship with iodination.
The presence of dimeric thyroglobulin (19S), after reduction and alkylation, suggests that within thyroglobulin there may be intermolecular cross-links, other than disulphide bridges. However, the nature of these intermolecular cross-links is still unknown. In this study, we show the presence of 3-3' dityrosine bridges in the molecule of bovine thyroglobulin by NMR and fluorescence studies. Also, we evaluated the role of iodination in dityrosine formation in vivo and in vitro.
Leonardi
A
A
Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, C.N.R. II Facoltà di Medicina e Chirurgia, Napoli, Italy.
Acquaviva
R
R
Marinaccio
M
M
Liguoro
D
D
Fogolari
F
F
Di Jeso
B
B
Formisano
S
S
Consiglio
E
E
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004220:Disulfides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D009682:Magnetic Resonance Spectroscopy; D011441:Propylthiouracil / Q000494:pharmacology; D013954:Thyroglobulin / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D013961:Thyroid Gland / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D004220:Disulfides; D014443:Tyrosine; D011441:Propylthiouracil; D013954:Thyroglobulin; C007543:dityrosine