Cloning of a novel putative protein kinase having a leucine zipper domain from human brain.
We report a novel putative serine/threonine protein kinase containing a leucine-zipper domain, isolated from an human neuronal cell line. The teratocarcinoma cell line NT2 was differentiated to postmitotic NT2-N neurons by treatment with retinoic acid, and degenerate oligonucleotide primers to the catalytic domains of protein kinases were employed to PCR amplify subtractive cDNAs. We identified a clone, represented at higher abundance in NT2-N neurons than in the parental cell line, which encodes a putative serine/threonine kinase of 859 [corrected] amino acids, the leucine-zipper protein kinase (zpk). Zpk protein contains a leucine-zipper domain, found in many DNA-binding proteins, but few protein kinases. Steady-state mRNA levels for zpk are high in human brain and kidney. Further studies are required to evaluate the role of zpk in neuronal differentiation.
Children's Hospital of Philadelphia, PA 19104.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D006801:Humans; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D009474:Neurons / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013724:Teratoma; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D017346:Protein Serine-Threonine Kinases