Minisatellite DNA-binding proteins in mouse brain, liver, and kidney.
Minisatellites are tandemly repeated DNA sequences that are found in most higher eukaryotes. They are genetically unstable and often gain or lose repeat units. Minisatellite repeats contain a "core" sequence which is highly conserved among a family of minisatellites. The core sequence resembles the chi sequence of Escherichia coli which is a binding site for recombination proteins. It has therefore been suggested that minisatellite core sequences may also be binding sites for proteins involved in recombination. In this paper, we report several proteins in mouse brain, liver, and kidney nuclear extracts which bind to various minisatellite sequences. We have found several proteins which have not been previously reported and, in addition, have noted that brain has a different profile of minisatellite-binding proteins than liver and kidney. Moreover, we have also observed probe-specificity in the binding of some of these proteins, suggesting that different "families" of minisatellites may have qualitatively different functions.
Department of Safety Assessment, Merck Research Laboratories, West Point, Pennsylvania 19486.
G A
GA
Shinder
S
S
Manam
B J
BJ
Ledwith
W W
WW
Nichols
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D017124:Conserved Sequence; D004276:DNA, Satellite / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D015151:Immunoblotting; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism; D015345:Oligonucleotide Probes; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes