An inexpensive method for the rapid identification of specific cDNAs from cDNA libraries.
The identification of specific cDNAs by screening filters impregnated with DNA from phage or bacterial colonies is a time-consuming and expensive undertaking. We have developed a rapid and inexpensive method for identifying specific cDNAs that involves hybridization of cDNAs to specific oligonucleotides bound to nylon filters. Using this method, we have identified specific cDNAs for a human B-cell growth factor from different cDNA libraries. The total processing time was 3 days, and 30% of the cDNAs selected by this method were correct.
Washington University School of Medicine, St. Louis, MO.
J
J
Pippin
V
V
Contractor
J L
JL
Ambrus
D000327:Adsorption; D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry; D002051:Burkitt Lymphoma / Q000235:genetics; D018076:DNA, Complementary / Q000302:isolation & purification*; D015723:Gene Library; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics; D007526:Isoelectric Point; D008567:Membranes, Artificial; D051379:Mice; D009693:Nucleic Acid Hybridization; D009757:Nylons; D010957:Plasmids; D013601:T-Lymphocytes / Q000737:chemistry
D018076:DNA, Complementary; D008567:Membranes, Artificial; D009757:Nylons; D015847:Interleukin-4