Human and rat TR4 orphan receptors specify a subclass of the steroid receptor superfamily.
We have identified a member of the steroid receptor superfamily and cloned it from human and rat hypothalamus, prostate, and testis cDNA libraries. The open reading frame between first ATG and terminator TGA can encode 615 (human) and 596 (rat) amino acids with calculated molecular mass of 67.3 (human) and 65.4 (rat) kDa. The amino acid sequence of this protein, called TR4 orphan receptor, is closely related to the previously identified TR2 orphan receptor. The high homology between TR2 and TR4 orphan receptor suggests that these two orphan receptors constitute a unique subfamily within the steroid receptor superfamily. These two orphan receptors are differentially expressed in rat tissues. Unlike TR2 orphan receptors, the TR4 orphan receptor appears to be predominantly located in granule cells of the hippocampus and the cerebellum, suggesting that it may play some role(s) in transcriptional regulation in these neurons.
Chang
C
C
Department of Human Oncology, University of Wisconsin, Madison 53792.
Da Silva
S L
SL
Ideta
R
R
Lee
Y
Y
Yeh
S
S
Burbach
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D015723:Gene Library; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011987:Receptors, Steroid / Q000096:biosynthesis* / Q000235:genetics; D011988:Receptors, Thyroid Hormone; D012689:Sequence Homology, Nucleic Acid; D013728:Terminator Regions, Genetic; D013737:Testis / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; C490515:NR2C2 protein, human; D009419:Nerve Tissue Proteins; C092882:Nr2c2 protein, rat; D012333:RNA, Messenger; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone