Detection of the readthrough protein of barley yellow dwarf virus.
The single open reading frame (ORF) 5 encoding the 50-kDa protein of barley yellow dwarf virus PAV-IL (BYDV-PAV-IL) was expressed in bacteria, purified, and used as an immunogen/antigen to produce/screen antibodies specific to the 50-kDa protein. Two monoclonal antibodies (MAb PAV-IL-22 kDa and MAb PAV-IL-50 kDa) raised against BYDV-PAV-IL could specifically detect the presence of the 72-kDa readthrough protein in extracts from the BYDV-infected leaf tissue. The results suggest that ORF 5 (50-kDa protein) is translated by readthrough of ORF 3 (22-kDa coat protein) to produce the 72-kDa protein. The readthrough protein is thought to be a structural protein on the external surface of BYDV.
Department of Plant Pathology, University of Illinois at Urbana-Champaign 61801.
S L
SL
Cheng
L L
LL
Domier
C J
CJ
D'Arcy
D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D005814:Genes, Viral; D001467:Hordeum; D017793:Luteovirus / Q000235:genetics* / Q000276:immunology; D008970:Molecular Weight; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015678:Viral Structural Proteins