No limits on restriction.
The structure of the endonuclease R.PvuII in the absence and presence of its recognition site indicates how the interaction with DNA might proceed.
G
G
Riddihough
D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D011514:Proteus vulgaris / Q000201:enzymology; D013329:Structure-Activity Relationship
D004247:DNA; D015246:Deoxyribonuclease EcoRI; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases