A highly sensitive quantitative bioassay for human interleukin-11.
Human interleukin-11 (IL-11) is a cytokine with a broad spectrum of activity, similar to interleukin-6 (IL-6). However, its role in human disease is poorly understood, partly because of a lack of sensitive bioassays. A subclone (B9-11) obtained from the B9 hybridoma (which responds poorly to human IL-11) enabled us to develop a highly sensitive bioassay for human IL-11. B9-11 cells responded only to human IL-11 and IL-6 and not to other human cytokines using the same gp130 transducer chain (ciliary neurotrophic factor, leukemia inhibitory factor and oncostatin M) or to other human interleukins (interleukin-1 to interleukin-13), human hematopoietic cytokines (granulocyte colony stimulating factor, granulocyte-macrophage colony stimulating factor, colony stimulating factor-1) and various other human cytokines (interferon-alpha, tumor necrosis factor-alpha, tumor necrosis factor-beta, fibroblast growth factor and nerve growth factor). In addition, these cytokines did not interfere with the IL-11 response of B9-11 cells. IL-11-induced proliferation of B9-11 cells was unaffected by anti-murine IL-6 receptor mAb but inhibited by anti-gp130 mAb. Half-maximal proliferation of B9-11 cells was obtained with 30 pg/ml of recombinant IL-11, a concentration 300-fold lower than IL-11 concentrations known to be active on human cells. Finally, culturing of B9-11 cells with an anti-IL-6 mAb enabled us to measure the natural IL-11 produced by various cell lines. Thus, B9-11 cells should be useful for studies of IL-11 involvement in various human diseases as well as for a better understanding of the mechanisms of action of this cytokine.
Lu
Z Y
ZY
Institute for Molecular Genetics, CNRS, Montpellier, France.
Zhang
X G
XG
Gu
Z J
ZJ
Yasukawa
K
K
Amiot
M
M
Etrillard
M
M
Bataille
R
R
Klein
B
B
D000818:Animals; D001681:Biological Assay / Q000379:methods* / Q000706:statistics & numerical data; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D017370:Interleukin-11 / Q000032:analysis* / Q000096:biosynthesis / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D051379:Mice; D012680:Sensitivity and Specificity
D016207:Cytokines; D017370:Interleukin-11; D015850:Interleukin-6