To boldly glow ... applications of laser scanning confocal microscopy in developmental biology.
The laser scanning confocal microscope (LSCM) is now established as an invaluable tool in developmental biology for improved light microscope imaging of fluorescently labelled eggs, embryos and developing tissues. The universal application of the LSCM in biomedical research has stimulated improvements to the microscopes themselves and the synthesis of novel probes for imaging biological structures and physiological processes. Moreover the ability of the LSCM to produce an optical series in perfect register has made computer 3-D reconstruction and analysis of light microscope images a practical option.
Howard Hughes Medical Institute, Laboratory of Molecular Biology, University of Wisconsin, Madison 53706.
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D000818:Animals; D002080:Butterflies / Q000196:embryology; D003196:Computer Graphics; D004330:Drosophila / Q000196:embryology; D004625:Embryo, Nonmammalian / Q000166:cytology*; D006801:Humans; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D012617:Sea Urchins / Q000196:embryology