Characterization of a medium wavelength type DNA photolyase: purification and properties of photolyase from Bacillus firmus.
The gene for the apoenzyme of Bacillus firmus photolyase was cloned and sequenced. The enzyme was overproduced in Escherichia coli, purified, and characterized. It has the unique property of having the maximum activity over a wavelength range where all other known photolyases exhibit modest activity. The enzyme contains reduced FAD and methenyltetrahydrofolate and has an absorption and action spectrum peak at 410 nm, and it repairs DNA with a quantum yield of phi approximately 0.75.
Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina, Chapel Hill 27599.
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D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000302:isolation & purification* / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013057:Spectrum Analysis
D001426:Bacterial Proteins; D017931:DNA Primers; D011994:Recombinant Proteins; D004255:Deoxyribodipyrimidine Photo-Lyase