Increased urinary excretion of the oxidative DNA adduct, 8-hydroxydeoxyguanosine, as a possible early indicator of occupational cancer hazards in the asbestos, rubber, and azo-dye industries.
Oxidative damage to DNA has been suggested to contribute to a number of diseases including cancer. In order to study the relationship between oxidative damage to DNA and occupational exposures, urinary excretion of the oxidative DNA adduct, 8-hydroxydeoxyguanosine (8-OHdG), was determined in asbestos workers, rubber workers, azo-dye workers and controls. Levels of 8-OHdG in urinary samples were quantified by automated coupled-column high-performance liquid chromatography with electrochemical detection (HPLC-EC). The registered 8-OHdG levels were 1.40 +/- 0.56 mumol/mol creatinine in asbestos workers, 1.48 +/- 0.57 in rubber workers, 1.92 +/- 0.85 in azo-dye workers and 1.07 +/- 0.41 in controls (means +/- SD). Thus, 8-OHdG levels appeared to be significantly higher (p < 0.05) in each of the exposed groups than in the control group. Regression analysis revealed no important association between 8-OHdG excretion, age and smoking. These findings suggest that occupational exposures may contribute to an increased oxidative damage to human DNA and point to the possible use of urinary 8-OHdG assays in biomonitoring of biological effects of chemicals in selected industrial workplaces.
Department of Occupational and Environmental Medicine, Faculty of Health Sciences, Linköping University, Sweden.
C
C
Tagesson
D
D
Chabiuk
O
O
Axelson
B
B
Barański
J
J
Palus
K
K
Wyszyńska
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D001194:Asbestos; D015415:Biomarkers / Q000652:urine; D002617:Chemical Industry; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D007221:Industry; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000139:chemically induced* / Q000652:urine; D016273:Occupational Exposure / Q000032:analysis*; D012408:Rubber
D015415:Biomarkers; D001194:Asbestos; D000080242:8-Hydroxy-2'-Deoxyguanosine; D012408:Rubber; D003849:Deoxyguanosine