Studies on inactivation of pathogenic microorganisms in culture media and in bovine semen by photosensitive agents.
The application of three photosensitive agents for disinfection of bovine semen was investigated. Bovine microbial pathogens suspended in tissue culture medium and/or PBS and also added to bovine semen were exposed to the photosensitive agents followed by irradiation. Hematoporphyrin, hematoporphyrin derivative and thiopyronine were effective against bovine herpes virus-1, bovine viral diarrhoea virus, Mycoplasma bovigenitalium, Mycoplasma canadense, and Ureaplasma diversum in culture media. In addition, thiopyronine was effective against Leptospira pomona. Similar treatments were not effective against Leptospira hardjo, Mycoplasma bovis, or Campylobacter fetus subsp. venerealis. When microorganisms were added to bovine semen, only bovine herpes virus-1 was controlled by the photosensitive agents used at concentrations which did not appear harmful to sperm cells.
Agriculture Canada, Animal Diseases Research Institute, NEPEAN, Ont., Canada.
M D
MD
Eaglesome
A
A
Bielanski
W C
WC
Hare
H L
HL
Ruhnke
D000818:Animals; D002168:Campylobacter fetus / Q000187:drug effects; D002417:Cattle; D003470:Culture Media; D001908:Diarrhea Viruses, Bovine Viral / Q000187:drug effects; D017324:Hematoporphyrin Derivative / Q000633:toxicity; D006415:Hematoporphyrins / Q000633:toxicity; D007242:Herpesvirus 1, Bovine / Q000187:drug effects; D007919:Leptospira / Q000187:drug effects; D008027:Light; D008297:Male; D009174:Mycoplasma / Q000187:drug effects; D011754:Pyronine / Q000031:analogs & derivatives / Q000633:toxicity; D011838:Radiation-Sensitizing Agents / Q000633:toxicity*; D012661:Semen / Q000382:microbiology*; D014509:Ureaplasma / Q000187:drug effects
D003470:Culture Media; D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative; C005792:thiopyronine; D011754:Pyronine