[The use of rocket immunoelectrophoresis for determining glycoprotein in concentrated rabies vaccines].
Rocket immunoelectrophoresis (RIE) was shown to be useful for the evaluation of glycoprotein (GP) content in concentrated rabies vaccines, and disintegron B., a zwitterionic detergent made in this country, for treatment of the vaccines for these evaluations. The values of GP content obtained by RIE and single radial immunodiffusion test were similar. The highest values were obtained for all the vaccines tested with anti-PM GP serum compared with anti-ERA and Vnukovo-32 GP serum. When anti-PM and anti-Vnukovo-32 GP sera were used for RIE two components were revealed in the vaccines. All the preparations under study contained soluble GP.
Volkova
R A
RA
Runova
V F
VF
Romanova
L N
LN
Khrapova
I S
IS
El'bert
L B
LB
Mal'dov
D G
DG
D001622:Betaine / Q000031:analogs & derivatives; D002845:Chromatography; D003902:Detergents; D006023:Glycoproteins / Q000032:analysis*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis / Q000379:methods; D007202:Indicators and Reagents; D011819:Rabies Vaccines / Q000032:analysis* / Q000302:isolation & purification; D012995:Solubility; D014461:Ultracentrifugation; D014462:Ultrafiltration
D003902:Detergents; D006023:Glycoproteins; D007202:Indicators and Reagents; D011819:Rabies Vaccines; C066009:desintegron B; D001622:Betaine
Primenenie raketnogo immunoélektroforeza dlia opredeleniia glikoproteina v kontsentrirovannykh antirabicheskikh vaktsinakh.