Toxic and trophic effects of glial-derived factors on neuronal cultures.
To test the hypothesis that glial-derived factors exert differential effects on neurons in developing and aged brain, we treated NB2a/d1 neuroblastoma cells with conditioned medium (CM) obtained from C6 glioma cells. Undifferentiated NB2a/d1 cells rapidly (< 4 h) elaborated neurites in response to CM. Cells previously differentiated by 14 days treatment with 1 mM dbcAMP, which induces outgrowth of axonal neurites, exhibited no obvious effects when exposed to CM. By contrast, CM was toxic to cells that were treated with 1 mM dbcAMP for 7 days followed by 7 days in which dbcAMP had been reduced to 0.1 mM or withdrawn. These results highlight the potential of glial-derived factors to exert trophic and toxic effects depending upon neuronal differentiation state.
Laboratory for Cellular and Developmental Neurobiology, Mailman Research Center, McLean Hospital, Belmont, MA 02178.
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D003994:Bucladesine / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000378:metabolism / Q000494:pharmacology*; D009420:Nervous System / Q000187:drug effects* / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D003994:Bucladesine