Cloning of multiple forms of goldfish vimentin: differential expression in CNS.
In efforts to determine the primary structure of intermediate filament proteins in the goldfish visual pathway, we isolated clones from a retinal lambda gt11 cDNA expression library that represent goldfish vimentin. We show that there are at least two forms of goldfish vimentin, designated as vimentin alpha and vimentin beta. RNase protection assays indicate that vimentin alpha mRNA is expressed in low amounts in retina, optic nerve, and brain and in higher amounts in spinal cord. In contrast, vimentin beta mRNA is expressed in low amounts in retina, optic nerve, brain, and spinal cord and in very high amounts in eye lens. Immunohistochemical studies show that in the optic nerve, vimentin alpha is mainly restricted to blood vessels, meninges, and septa. Light staining is observed with this antibody in an astrocytic glial pattern throughout the optic nerve. Two-dimensional gel analysis shows that all of these goldfish vimentins are low abundant components of optic nerve cytoskeletal preparations.
Glasgow
E
E
Department of Biochemistry, Health Sciences Center, State University of New York, Stony Brook, New York 11794.
Druger
R K
RK
Fuchs
C
C
Levine
E M
EM
Giordano
S
S
Schechter
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002347:Carps; D002490:Central Nervous System / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000302:isolation & purification; D015870:Gene Expression; D006054:Goldfish; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D007908:Lens, Crystalline / Q000378:metabolism; D008969:Molecular Sequence Data; D009900:Optic Nerve / Q000378:metabolism; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D012160:Retina / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000378:metabolism; D014746:Vimentin / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D014981:Xenopus
D017931:DNA Primers; D018076:DNA, Complementary; D007381:Intermediate Filament Proteins; D012333:RNA, Messenger; D014746:Vimentin