Automated sequential trace enrichment of dialysates combined with high-performance liquid chromatography and automated heart-cutting for the determination of the nucleoside 1-(beta-D-arabinofuranosyl)-5-(1-propynyl)uracil and its metabolite 5-propynyluracil in urine.
The use of a new configuration to control the automated sequential trace enrichment of dialysate (ASTED) system has been used to estimate 1-(beta-D-arabinofuranosyl)-5-(1-propynyl)uracil and its metabolite 5-propynyluracil in urine. The system employs "heart-cutting" as a means to improve the efficiency of sample preparation and reduce analysis time. Using this technique the mean within- and between-run imprecision (coefficient of variation) at three different urine analyte concentrations was found to be 1.6 and 3.6% and 1.7 and 3.3% for 5-propynyluracil and 1-(beta-D-arabinofuranosyl)-5-(1-propynyl)uracil, respectively.
Cooper
J D
JD
Clinical Innovations Ltd., Kenilworth, Warwickshire, UK.
Sheung
C T
CT
Buick
A R
AR
D000480:Alkynes / Q000652:urine*; D000998:Antiviral Agents / Q000652:urine*; D001086:Arabinofuranosyluracil / Q000031:analogs & derivatives* / Q000652:urine; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D003201:Computers; D003956:Dialysis; D006801:Humans; D007202:Indicators and Reagents; D012984:Software; D013056:Spectrophotometry, Ultraviolet; D014498:Uracil / Q000031:analogs & derivatives* / Q000652:urine
D000480:Alkynes; D000998:Antiviral Agents; D007202:Indicators and Reagents; C083257:5-propynyluracil; D001086:Arabinofuranosyluracil; D014498:Uracil; C067854:5-propynylarabinofuranosyluracil