Heterologous expression of cDNAs encoding barley (Hordeum vulgare) (1-->3)-beta-glucanase isoenzyme GV.
Two cDNAs have been isolated from libraries generated from poly(A)+RNA of young barley roots and leaves, using a cDNA encoding barley (1-->3)-beta-glucanase isoenzyme GII as a probe. Nucleotide sequence analyses and ribonuclease protection assays show that the two cDNAs differ only in the length of their 3'-untranslated regions; the corresponding mRNAs are likely to originate from a single gene by tissue-specific processing at separate polyadenylation sites. When the coding region of the cDNA is expressed in E. coli, the resultant protein catalyses the hydrolysis of (1-->3)-beta-glucan with an action pattern characteristic of a (1-->3)-beta-glucan endohydrolase (EC 3.2.1.39). The enzyme has been designated isoenzyme GV of the barley (1-->3)-beta-glucanase family).
Department of Plant Science, University of Adelaide, South Australia.
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Xu
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Harvey
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Fincher
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D004926:Escherichia coli; D043326:Glucan 1,3-beta-Glucosidase; D001467:Hordeum / Q000201:enzymology*; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011061:Poly A / Q000378:metabolism; D011994:Recombinant Proteins; D015183:Restriction Mapping; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D007527:Isoenzymes; D011994:Recombinant Proteins; D011061:Poly A; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase