The biomechanics and histopathology of chemically processed patellar tendon allografts for anterior cruciate ligament replacement.
A study was initiated to examine chemically processed patellar tendon allografts in sheep anterior cruciate ligament repairs, both mechanically and histologically. One group of animals received frozen, untreated allografts, one group received frozen grafts that were processed with a chloroform-methanol solvent extraction technique, and one group received frozen tendons treated with a permeation-enhanced extraction technique. All animals were operated on unilaterally, with the contralateral knee acting as a normal, intact control. Histologic analysis after 2 months of implantation revealed similar enhanced cellular repopulation in both chemically treated ligament allografts compared with the more hypocellular, untreated grafts. At 6 months the chloroform-methanol group demonstrated a more aggressive chronic cellular response with numerous thick-walled vessels relative to the untreated and permeation-enhanced grafts. Mechanical testing after 6 months of implantation showed statistically similar anterior drawer resistance in all grafted knees, yet the two chemically extracted grafts had significantly less stiffness than untreated anterior cruciate ligament grafts. Both treatment groups also tended to be weaker than the untreated allografts. All anterior cruciate ligament reconstructions showed excessive anterior drawer laxity and, regardless of treatment, had lower strength and less stiffness than normal anterior cruciate ligament tissue at the 6-month period.
Department of Orthopaedics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
M C
MC
Zimmerman
J H
JH
Contiliano
J R
JR
Parsons
A
A
Prewett
J
J
Billotti
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000601:surgery*; D001696:Biomechanical Phenomena; D001808:Blood Vessels / Q000473:pathology; D016723:Bone Remodeling; D016025:Bone Transplantation; D002358:Cartilage, Articular / Q000473:pathology; D002725:Chloroform; D003094:Collagen; D004548:Elasticity; D005260:Female; D005269:Femur / Q000473:pathology / Q000601:surgery; D005347:Fibroblasts / Q000473:pathology; D005615:Freezing; D000432:Methanol; D017847:Patellar Ligament / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D012756:Sheep; D013314:Stress, Mechanical; D013997:Time Factors; D014021:Tissue Preservation; D014184:Transplantation, Homologous; D016474:Weight-Bearing
D002725:Chloroform; D003094:Collagen; D000432:Methanol