Phase behavior of the antineoplastic ether lipid 1-O-octadecyl-2-O-methyl-glycero-3-phosphochloline.
The physicochemical properties of the antineoplastic etherphospholipid 1-O-octadecyl-2-O-methyl-glycero-3-phosphochline were examined in the concentration range 1-35% (w/w) lipid, as a function of temperature (range -10 degrees C to 40 degrees C) and of different aqueous solvents by dynamic light scattering, small- and wide-angle X-ray scattering, differential scanning calorimetry and ultrasonic speed measurements. On cooling the lipid dispersion undergoes a phase transition near 6 degrees C, transforming slowly from a micellar into a lamellar gel phase with interdigitating hydrocarbon chains. The lamellar repeat distance is nearly constant over the hydration range 65-90% buffer (d = 5.09-5.14 nm). The size of the micelles in terms of the hydrodynamic radius is 3.8 +/- 0.1 nm, the polydispersity is low. Their average shape is spherical. The electron density distribution across the micelle gives 2.5 nm for the extension of the hydrocarbon chains and 1.5 nm for the polar moiety. The existence of micelles was verified up to a concentration of 35% lipid. Throughout this concentration range size and shape do not change significantly. The kinetics of formation of the low-temperature phase is slow on cooling, increasing with increasing concentration. Upon heating the phase behavior shows a hysteresis. The extended lamellar organizations start to break down into smaller aggregates near 3 degrees C. The micellar phase is reformed near 20 degrees C.
Institute of Physical Chemistry, University of Graz, Austria.
N
N
Maurer
E
E
Prenner
F
F
Paltauf
O
O
Glatter
D000970:Antineoplastic Agents / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D008823:Micelles; D010742:Phospholipid Ethers / Q000737:chemistry*; D012997:Solvents; D013696:Temperature; D014961:X-Ray Diffraction
D000970:Antineoplastic Agents; D008823:Micelles; D010742:Phospholipid Ethers; D012997:Solvents; C026659:edelfosine