Radiation-induction of micronuclei in human peripheral blood lymphocytes: effect of freezing.
Lymphocytes in peripheral blood samples from three donors were irradiated at doses of 0, 1 and 2 Gy with cobalt-60 gamma rays. Whole blood cultures were established immediately after irradiation. Cultures of Ficoll-Paque separated lymphocytes were set up immediately after irradiation, or 3 weeks after storage in liquid nitrogen at -196 degrees C. The frequency of micronuclei was measured in binucleate cytokinesis-blocked cells and the different treatment groups were compared. No clastogenic effect of the lymphocyte separation technique, or of subsequent freezing in liquid nitrogen, was observed.
Prosser
J S
JS
Biomedicine and Health Program, Australian Nuclear Science and Technology Organisation, Menai, NSW.
Izard
B E
BE
Wallace
M A
MA
D000328:Adult; D002467:Cell Nucleus / Q000528:radiation effects / Q000648:ultrastructure; D002478:Cells, Cultured; D015925:Cryopreservation; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects* / Q000648:ultrastructure*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects* / Q000648:ultrastructure*; D013997:Time Factors