Rabbit knee-joint meniscal proteoglycans.
A major proteoglycan (PG) in rabbit meniscal fibrocartilage was extracted with 4 M guanidine-HCl. It represented 63% of the total uronic acid originally present in the tissue. The non-extractable PG could be released by treating the guanidine HCl-extracted tissue with trypsin followed by alkaline hydrolysis. The glycosaminoglycan (GAG) chains from the non-extractable PGs are shown to be of a different type than the extractable forms since the relative amounts of chondroitin sulphate isomers differ from each other.
Cell Biology Division, Escola Paulista de Medicina, Rua Botucatu, São Paulo, Brazil.
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D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002810:Chondroitinases and Chondroitin Lyases; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D006025:Glycosaminoglycans / Q000737:chemistry; D019791:Guanidine; D006146:Guanidines; D007596:Joints / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification; D011817:Rabbits; D014357:Trypsin
D004187:Disaccharides; D006025:Glycosaminoglycans; D006146:Guanidines; D011509:Proteoglycans; D014357:Trypsin; D002810:Chondroitinases and Chondroitin Lyases; D019791:Guanidine