Synergistic effect of choline and carnitine on acetylcholine synthesis in neuroblastoma NB-2a cells.
An influence of carnitine on acetylcholine synthesis from radiolabeled glucose was monitored in neuroblastoma NB-2a cells. Upon addition of carnitine the distribution of its derivatives was found significantly different than the values published for brain, the level of long-chain acyl derivatives being much higher and reaching 60%. Carnitine itself did not change acetylcholine level. Together with choline (20 microM), carnitine was observed to stimulate (by 36%) acetylcholine synthesis in a synergistic way, which indicated that both substrates could be limiting factors of this process in NB-2a cell line of neuroblastoma.
Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa.
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D000109:Acetylcholine / Q000378:metabolism*; D000215:Acylation; D000818:Animals; D002331:Carnitine / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D002794:Choline / Q000378:metabolism / Q000494:pharmacology*; D004357:Drug Synergism; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D002794:Choline; D000109:Acetylcholine; D002331:Carnitine