[S antigen in retinas of royal college of surgeons rats: time course and retinal pigment epithelium transplant study].
Retinas of Royal College of Surgeons (RCS) rats were investigated immunocytochemically for the distribution of S antigen prior to and during the retinal disease process. RCS retinas onto which retinal pigment epithelium (RPE) had been transplanted were also immunostained with antibody for S antigen. At P2, weak immunostaining for S antigen was seen in neuroepithelium. At P22, the staining pattern outlined the entire photoreceptor layer. At P30, at the beginning of retinal degeneration, immunostaining for S antigen was considerably reduced in the degenerating photoreceptor outer segment. In the RCS retina at P90, immunostaining for S antigen was completely lost. However, retinas with transplanted RPE showed preserved immunocytochemical staining for S antigen at this late stage. These results suggest a loss of production of S antigen in degenerated retinal tissue, and that RPE transplantation helps to preserve S antigen in the RCS rat retina.
Department of Ophthalmology, School of Medicine, Tohoku University, Japan.
Y
Y
Takeda
K
K
Yamaguchi
K
K
Yamada
M
M
Tamai
D000818:Animals; D000941:Antigens / Q000378:metabolism*; D019393:Arrestin; D005136:Eye Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D010857:Pigment Epithelium of Eye / Q000637:transplantation*; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000276:immunology*; D012162:Retinal Degeneration / Q000378:metabolism; D013997:Time Factors
D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins