[Immunomorphocytochemical and immunofluorescent study methods in the immunodiagnosis of acute leukemias].
Immunophenotyping results were compared for blast cells of the bone marrow and blood in a wide panel of monoclonal antibodies to differentiating antigens of the cells in various stages of hemopoiesis. The study was performed in acute leukemia (AL) patients using the immunocytochemical investigation (APAAP) and immunofluorescence (IF). Morphological evaluation of the marrow and blood involved cytochemical tests on glycogen, lipids, acid sulfated mucopolysaccharides, measurements of lysosomal enzymes and myeloperoxidase activities. Immunophenotyping was made with APAAP and IF. The results of the latter appeared approximately similar. APAAP proved more sensitive and informative in determination of blast cell phenotype and probable level of differentiation block. This may be used for earlier differential immunodiagnosis of acute leukemia variants. The immunological variants were found to correlate with some cytochemical indices pointing to the validity of more profound acute lymphoblastic leukemia classification to improve the diagnosis and treatment protocol designing.
Shakhbazian
G P
GP
Miterev
G Iu
GIu
Bulycheva
T I
TI
D000208:Acute Disease; D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000276:immunology; D003937:Diagnosis, Differential; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007159:Immunologic Tests / Q000379:methods; D016130:Immunophenotyping; D007938:Leukemia / Q000175:diagnosis*
D000911:Antibodies, Monoclonal
Immunomorfotsitokhimicheskie i immunofliuorestsentnye metody issledovaniia v immunodiagnostike ostrykh leĭkozov.