Heterologous gene expression and protein secretion from Candida glabrata.
We have examined heterologous protein secretion from Candida glabrata with the aid of a stable C. glabrata vector and a secretion reporter cassette comprising the Saccharomyces cerevisiae PGK-gene promoter and a Kluveromyces Iactis secretion signal to drive secretion of Escherichia coli beta-lactamase. Abundant secretion of beta-lactamase from C. glabrata indicates that the S. cerevisiae PGK promoter functions in C. glabrata. Furthermore, we show that C. glabrata processes the secreted beta-lactamase in a manner similar to, but not identical with, S. cerevisiae and K. lactis. C. glabrata may be a suitable new host for the expression of foreign genes.
Macreadie
I G
IG
Biomolecular Research Institute, Parkville, Victoria, Australia.
Castelli
L A
LA
Mehra
R K
RK
Thorvaldsen
J L
JL
Winge
D R
DR
D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D008969:Molecular Sequence Data; D010735:Phosphoglycerate Kinase / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D001618:beta-Lactamases / Q000378:metabolism
D004274:DNA, Recombinant; D011994:Recombinant Proteins; D010735:Phosphoglycerate Kinase; D001618:beta-Lactamases