Liver fatty acid composition correlates with body fat and sex in a multigenic mouse model of obesity.
To determine whether there is altered liver lipid-fraction fatty acid distribution in a multigenic obese mouse model, we examined livers from eight lean (0.2-4.2% carcass fat), seven intermediate (5.7-13.8%), and five obese (20.2-48.7%) backcross progeny [(C57BL/6J x Mus spretus) x C57BL/6J] aged 2-3 mo. Thirteen males and seven females were fed a nonpurified stock diet. Liver lipid fractions were separated and fatty acids quantitated by thin-layer and gas chromatography. There was a significant effect of obesity on 18:2 omega 6 in liver phospholipids (PL), cholesteryl esters, and triglycerides. PL 18:2 omega 6 was negatively correlated with carcass fat (r = -0.74, P < 0.001); 20:3 omega 6 was elevated in PL with increased obesity (P < 0.0001), and was correlated with carcass fat (r = 0.92, P < 0.0001); and 20:4 omega 6 in PL did not differ with obesity status. PL 20:3 omega 6 and 20:4 omega 6 were lower in males (P < 0.01 and 0.02, respectively) than in females. We conclude that obesity and sex affect distribution of omega 6 essential fatty acids in mouse liver lipid fractions.
Phinney
S D
SD
Department of Medicine, University of California at Davis 95616.
Fisler
J S
JS
Tang
A B
AB
Warden
C H
CH
D000818:Animals; D001823:Body Composition; D001835:Body Weight; D002788:Cholesterol Esters / Q000032:analysis / Q000378:metabolism; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005228:Fatty Acids, Essential / Q000032:analysis / Q000378:metabolism*; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000378:metabolism; D005260:Female; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D051379:Mice; D008820:Mice, Obese; D008954:Models, Biological; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism; D012737:Sex Factors; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism
D002788:Cholesterol Esters; D005228:Fatty Acids, Essential; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D014280:Triglycerides