Survival and regeneration of adult human photoreceptors in vitro.
Adult human retina from enucleated ocular tissue was enzymatically dissociated and plated into plastic dishes for cell culture in serum-supplemented medium. Within a few days following seeding, islands of glial-like cells with rounded neurons growing on top of them were visible. Immunocytochemical labelling of these cultures revealed that virtually all the surviving neurons (> 98%) were rod photoreceptors, and that they extended long neurites across the glial cell surface. Hence, adult human photoreceptors retain a remarkable capacity for survival and regrowth, and such preparations may be of value for physiopathological and retinal grafting studies.
Laboratoire Laveran, INSERM CJF 92/02, Hôpital Civil, Strasbourg, France.
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D000906:Antibodies; D015415:Biomarkers / Q000032:analysis; D002256:Carbonic Anhydrases / Q000032:analysis; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D010786:Photoreceptor Cells / Q000166:cytology*; D012160:Retina; D012243:Rhodopsin / Q000032:analysis
D000906:Antibodies; D015415:Biomarkers; D012243:Rhodopsin; D002256:Carbonic Anhydrases