Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting.
Deletion of the promoter and the first exon of the DNA polymerase beta gene (pol beta) in the mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived, site-specific recombinase Cre in a transgenic approach, the same mutation can be selectively introduced into a particular cellular compartment-in this case, T cells. The impact of the mutation on those cells can then be analyzed because the mutant animals are viable.
Gu
H
H
Institute for Genetics, University of Cologne, Germany.
Marth
J D
JD
Orban
P C
PC
Mossmann
H
H
Rajewsky
K
K
D000818:Animals; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D004256:DNA Polymerase I / Q000235:genetics* / Q000378:metabolism; D005260:Female; D017353:Gene Deletion; D005818:Genetic Engineering / Q000379:methods*; D006720:Homozygote; D019426:Integrases; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009154:Mutation; D011995:Recombination, Genetic; D013234:Stem Cells / Q000201:enzymology; D013601:T-Lymphocytes / Q000201:enzymology*; D014162:Transfection; D014764:Viral Proteins
D014764:Viral Proteins; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; D019426:Integrases; D004256:DNA Polymerase I