Some enzymes of gluconeogenesis in various fractions of sarcocysts of Sarcocystis fusiformis of buffalo (Bubalus bubalis).
A biochemical study was conducted to assess the relative presence of some enzymes of gluconeogenesis in various fractions--the cyst wall, cyst fluid and zoites--of sarcocysts of Sarcocystis fusiformis obtained from the oesophageal muscles of naturally infected Indian water buffalo (Bubalus bubalis). The activities of fructose 1,6-diphosphatase and malic enzyme were beyond detectable limits. Phosphoenol pyruvate carboxykinase (PEPCK) activity was maximally present in the zoites, whereas glucose 6-phosphatase activity was highest in the cyst wall. PEPCK seemed to play a crucial role in carbon dioxide fixation metabolism.
Department of Biochemistry, College of Veterinary Science and Animal Husbandry, Jabalpur, India.
R S
RS
Gupta
H S
HS
Kushwah
A
A
Kushwah
D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D006597:Fructose-Bisphosphatase / Q000032:analysis; D005943:Gluconeogenesis; D005952:Glucose-6-Phosphatase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000032:analysis; D012522:Sarcocystis / Q000201:enzymology*; D012523:Sarcocystosis / Q000469:parasitology / Q000662:veterinary*
D008291:Malate Dehydrogenase; C030574:malate dehydrogenase (decarboxylating); D006597:Fructose-Bisphosphatase; D005952:Glucose-6-Phosphatase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)