Deoxyribonucleic acid hybridization method for the detection of Listeria in dairy products, seafoods, and meats: collaborative study.
The method is based on the hybridization of synthetic deoxyribonucleic acid probes to ribosomal ribonucleic acid sequences unique to Listeria. This method was compared to 2 culture methods: the U.S. Food and Drug Administration method for the detection of Listeria in dairy products and seafoods and the U.S. Department of Agriculture, Food Safety and Inspection Service method for Listeria in meats. Six food types with replicate samples containing various concentrations of Listeria were analyzed by the collaborating laboratories. Listeria was detected in 774 samples using the DNAH method and in 772 samples using a culture method. The DNAH and culture methods were in agreement for 668 samples containing Listeria and 306 samples without Listeria. The overall rate of agreement between methods was 82.3%. The method has been adopted first action by AOAC INTERNATIONAL.
Silliker Laboratories Group, Inc., Chicago Heights, IL 60411.
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D000818:Animals; D003386:Brachyura / Q000382:microbiology; D002417:Cattle; D002611:Cheese / Q000382:microbiology; D003124:Colorimetry / Q000706:statistics & numerical data; D004269:DNA, Bacterial / Q000032:analysis*; D003611:Dairy Products / Q000382:microbiology*; D005516:Food Microbiology; D008087:Listeria / Q000235:genetics / Q000302:isolation & purification*; D008460:Meat / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D009693:Nucleic Acid Hybridization / Q000379:methods* / Q000706:statistics & numerical data; D017747:Seafood / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013552:Swine
D004269:DNA, Bacterial