Removing anchorage-dependent cells from culture flasks.
We have described an inexpensive and simple method for removal of monolayered cells from tissue culture flasks that, when combined with the reusable procedure of Shepard and Hartmann (2), will save both time and money in laboratories on a limited budget or that do not desire to use a rubber policeman.
H.L. Snyder Memorial Research Foundation, Winfield, KS.
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Zeng
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Smith
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Mack
D000264:Adenylosuccinate Lyase / Q000032:analysis; D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002498:Centrifugation; D006224:Cricetinae; D005898:Glass; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008863:Microspheres
D007770:L-Lactate Dehydrogenase; D000264:Adenylosuccinate Lyase