The sensitivity of head and neck squamous cell carcinomas to tumor necrosis factor-alpha.
Sensitivities to recombinant human tumor necrosis factor-alpha (TNF-alpha) and chemotherapeutic agents (cisplatin, peplomycin, methotrexate) were evaluated in 20 tumor cells of head and neck squamous cell carcinomas, using a dye uptake method. Also, numbers of TNF receptors of these tumor cells were measured by Scatchard plot analysis. There was no relationship between the number of TNF-alpha receptors and the sensitivity to TNF-alpha. Furthermore, there was no correlation between the sensitivity to TNF-alpha and that to chemotherapeutic drugs, nor between the sensitivity to TNF-alpha and the clinical response to chemotherapy including of cisplatin and peplomycin. The sensitivity to TNF-alpha was higher in poorly differentiated carcinomas than in well differentiated ones.
Department of Otorhinolaryngology, Yokoham City University, School of Medicine, Kanagawa, Japan.
I
I
Mochimatsu
M
M
Tsukuda
S
S
Furukawa
S
S
Watanabe
A
A
Kubota
S
S
Yanoma
D000970:Antineoplastic Agents / Q000633:toxicity; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000648:ultrastructure; D004354:Drug Screening Assays, Antitumor; D006258:Head and Neck Neoplasms / Q000188:drug therapy* / Q000648:ultrastructure; D006801:Humans; D007700:Kinetics; D018124:Receptors, Tumor Necrosis Factor / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000633:toxicity*
D000970:Antineoplastic Agents; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha