Myelinated axons demonstrated in the CNS and PNS by anti-neurofilament immunoreactivity and Luxol fast blue counterstaining.
A method which demonstrates myelinated axons in the central and peripheral nervous systems will be described. Paraffin, frozen or semi-thin epoxy-embedded sections were immunostained first with a monoclonal antibody raised against a 200 kilo-Dalton neurofilament protein and then counter-stained with Luxol fast blue.
Laboratory of Experimental Neuropathology, National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
D L
DL
Yao
S
S
Komoly
Q L
QL
Zhang
H D
HD
Webster
D000578:Amidines; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001369:Axons / Q000648:ultrastructure*; D002490:Central Nervous System / Q000166:cytology*; D006801:Humans; D007211:Indoles; D007382:Intermediate Filaments / Q000648:ultrastructure*; D051379:Mice; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure*; D016900:Neurofilament Proteins / Q000032:analysis* / Q000276:immunology; D010525:Peripheral Nerves / Q000166:cytology*; D051381:Rats
D000578:Amidines; D000911:Antibodies, Monoclonal; D007211:Indoles; D016900:Neurofilament Proteins; C031455:diamidino compound 253-50; C018588:Luxol Fast Blue MBS