Detailed analysis of a family of RNAs transcribed from varicella-zoster virus open reading frame 21 during replication.
The expression of VZV open reading frame (ORF) 21 during viral replication was examined at the RNA level. Three distinct mRNAs homologous to ORF 21, 3.6, 3.0, and 2.4 kb in size, were detected by Northern blot hybridization. Sequence analysis of eight ORF 21 cDNAs indicated that the corresponding RNAs were 3' coterminal and unspliced. The three largest cDNAs contained the entire ORF 21 coding sequence. Two upstream alternative polyadenylation signals located within ORF 21 were not utilized. Sequence analysis of the site of polyadenylation suggests a virus-specific cis-acting element is present in these transcripts.
Department of Neurology, University of Colorado School of Medicine, Denver 80262.
K J
KJ
Gilligan
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D014645:Herpesvirus 3, Human / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D015678:Viral Structural Proteins / Q000235:genetics*; D014779:Virus Replication
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins; D011061:Poly A