Characterization of the CNRE-1 family of repetitive DNA elements in Cryptococcus neoformans.
The pathogenic yeast Cryptococcus neoformans contains 10-20 dispersed repetitive elements that hybridize to clone CNRE-1.0. Screening of a genomic library with probes derived from CNRE-1.0 identified five phages with restriction maps that overlapped CNRE-1.0 and three additional phages that belonged to two distinct groups. Sequencing of internal 3.5-kb SstI fragments from two CNRE-1-like elements revealed 95% homology, as well as a conserved open reading frame. A PCR-RFLP assay was developed that can distinguish different subfamilies of CNRE-1-like elements.
Spitzer
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Department of Pathology, SUNY at Stony Brook 11794-8691.
Spitzer
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D001483:Base Sequence; D003455:Cryptococcus neoformans / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D005784:Gene Amplification; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal