Adaptation of signal transduction in brain.
Cell culture models were used to study the effects of long-term ethanol exposure on neuronal cells. Effects on phospholipase C and phospholipase D mediated signal transduction were investigated by assaying receptor-binding, G protein function, activities of lipases, formation of second messengers and c-fos mRNA. The signal transduction cascades displayed abnormal activities from 2 to 7 days of exposure which differed from the acute effects. Phosphatidylethanol formed by phospholipase D is an abnormal lipid that may harmfully affect nerve cell function.
Alling
C
C
Dept. of Psychiatry and Neurochemistry, Lund University, Sweden.
Gustavsson
L
L
Larsson
C
C
Lundqvist
C
C
Rodriguez
D
D
Simonsson
P
P
D000064:Acclimatization; D000818:Animals; D001921:Brain / Q000502:physiology*; D002460:Cell Line; D004075:Diglycerides / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D019204:GTP-Binding Proteins / Q000378:metabolism; D016762:Genes, fos; D005910:Glioma; D006801:Humans; D006822:Hybrid Cells; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D008959:Models, Neurological; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010739:Phospholipase D / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D015290:Second Messenger Systems; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism
D004075:Diglycerides; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D000431:Ethanol; D015544:Inositol 1,4,5-Trisphosphate; D008049:Lipase; D010738:Type C Phospholipases; D010739:Phospholipase D; D019204:GTP-Binding Proteins