Cloning and sequence analysis of a recA-like gene from Streptomyces venezuelae ISP5230.
When genomic DNA fragments from Streptomyces venezuelae ISP5230 were probed at moderate stringency with recA from Mycobacterium tuberculosis, a 2.0-kb SmaI fragment was identified. The fragment was isolated by cloning a BamHI digest of S. venezuelae DNA in pHJL400 and screening the plasmids in Escherichia coli by Southern hybridization using a sib-selection technique. Sequencing the hybridizing region located an open reading frame encoding 377 amino acids. Its deduced amino acid sequence resembled that of recA genes from other bacteria. The cloned S. venezuelae gene conferred partial resistance to ethyl methanesulfonate when expressed in E. coli from the lacZ promoter.
Yao
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Biology Department, Dalhousie University, Halifax, Nova Scotia, Canada.
Vining
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011938:Rec A Recombinases / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics*
D004269:DNA, Bacterial; D005020:Ethyl Methanesulfonate; D011938:Rec A Recombinases