Effects of slaframine and 4-diphenylacetoxy-N-methylpiperidine methiodide (4DAMP) on pancreatic exocrine secretion in the bovine.
Three Holstein steers (345 +/- 22 kg) surgically fitted with a pancreatic cannula were used in two 3 x 3 Latin square design experiments to examine the effects of slaframine (SF), a muscarinic agonist, or 4-diphenylacetoxy-N-methylpiperidine methiodide (4DAMP), an M3 muscarinic glandular receptor antagonist, on pancreatic exocrine secretion. Pancreatic exocrine secretion was collected for 8 h postdosing at 30-min intervals beginning 1 h postfeeding. In experiment 1, steers were dosed with 0, 25, or 50 micrograms.kg-1 body weight (BW) of SF. Secretion of pancreatic juice and the pH of the secreted juice increased linearly (p < 0.05) with SF; however, secretion rate showed a time by treatment interaction (p < 0.05), as treatments converged 7 h postdosing. Trypsin secretion tended (p < 0.10) to show a quadratic response to SF administration, with the 25 micrograms SF.kg-1 BW dose having the lowest value. In experiment 2, steers received 50 micrograms.kg-1 BW of SF (positive control), 113 micrograms.kg-1 BW of 4DAMP (isosmolar with SF), or both. SF caused a greater pancreatic fluid secretion (p < 0.10) than 4DAMP, with SF plus 4DAMP intermediate. A time by treatment interaction (p < 0.04) was found, since treatments converged 8 h postdosing. Trypsin secretion was higher (p < 0.05) for SF than the other treatments. Chymotrypsin, alpha-amylase, and protein secretion were not affected. SF and 4DAMP alter pancreatic fluid secretion in the steer but have minimal effects on enzyme secretions.
Walker
J A
JA
Department of Animal Sciences, University of Kentucky, Lexington 40546-0215.
Krehbiel
C R
CR
Harmon
D L
DL
St Jean
G
G
Croom
W J
WJ
Hagler
W M
WM
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008297:Male; D018727:Muscarinic Antagonists; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism; D010276:Parasympatholytics / Q000494:pharmacology*; D010277:Parasympathomimetics / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism
D000470:Alkaloids; D018727:Muscarinic Antagonists; D010276:Parasympatholytics; D010277:Parasympathomimetics; D010880:Piperidines; D011506:Proteins; C009275:slaframine; C042375:4-diphenylacetoxy-1,1-dimethylpiperidinium; D000516:alpha-Amylases; D002918:Chymotrypsin