Dependence of cholecystokinin-8-stimulated insulin release on high glucose levels is evidenced by pseudo-alpha-D-glucose in rat pancreas and islets.
The glucose-dependency of cholecystokinin-8 (CCK-8)-stimulated insulin release was investigated at high (11.1 and 16.7 mM) glucose concentrations in rat pancreas perfusion and islet perifusion using glucose analogues, pseudo-alpha-D-glucose and 2-deoxy-D-glucose. In perfused pancreas, both glucose analogues (22.4 mM) inhibited high glucose (16.7 mM)-induced insulin release, but not normal glucose (5.6 mM)-induced insulin release, with or without CCK-8 (1 nM). In perifused islets, the same level of either of the glucose analogues inhibited high glucose (11.1 mM)-induced insulin release, with or without CCK-8 (100 nM). These results demonstrate that CCK-8-stimulated insulin release only at high glucose level is glucose-dependent.
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
I
I
Kimura
N
N
Nakashima
T
T
Komori
Y
Y
Kameda
M
M
Kimura
D000818:Animals; D003847:Deoxyglucose / Q000378:metabolism / Q000494:pharmacology; D005947:Glucose / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D012844:Sincalide / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D013268:Stimulation, Chemical
D007328:Insulin; C048030:5-hydroxymethyl-1,2,3,4-cyclohexanetetrol; D003847:Deoxyglucose; D005947:Glucose; D012844:Sincalide