Synthetic peptide corresponding to 30 amino acids of the C-terminal of neuron-specific enolase promotes survival of neocortical neurons in culture.
Neuron-specific enolase (NSE), one of the glycolytic enzymes, is a gamma gamma-isozyme of enolase that is specifically expressed in neurons. Our previous studies demonstrated that NSE promotes survival of rat embryonic neocortical neurons in culture but that the alpha alpha-isozyme (non-neuronal enolase; NNE) has no effect. In this study, we found that a synthetic peptide corresponding to the C-terminal portion of NSE (404-433) also promotes the survival of neocortical neurons. By contrast, a synthetic peptide of the C-terminal portion of NNE (404-433) has no effect on neuronal survival. These findings would be important for further analysis of the neurotrophic mechanism of NSE.
Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan.
T
T
Hattori
K
K
Ohsawa
Y
Y
Mizuno
K
K
Kato
S
S
Kohsaka
D000595:Amino Acid Sequence; D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D004622:Embryo, Mammalian; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D008869:Microtubule-Associated Proteins / Q000032:analysis; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010751:Phosphopyruvate Hydratase / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats
D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D010455:Peptides; D010751:Phosphopyruvate Hydratase