Ascorbic acid enhances the decrease in peroxidase activity in inflamed tissues of mice.
The aim of this study was to explore whether intraperitoneal administration of ascorbic acid (AA) at a dose of 500 mg/kg, once a day for 3 following days, affected the peroxidase (PO) activity in inflamed feet of mice. The foot inflammatory reaction induced by the carrageenan (CAR), n-formyl-methionyl-leucyl-phenylalanine (FMLP) and xanthine-xanthine oxidase was accompanied by suppression of PO activity. Administration of AA, having no effect on the degree of foot oedema, skin temperature and microscopic picture of tissue specimens significantly enhanced the decline in PO activity provoked by inflammatory agents. This activity decreased 2.0-, 1.6- and 1.9-fold (p < 0.001, p < 0.01, p < 0.05) when inflammatory response was induced with FMLP, CAR and X-XO, respectively. Also in vitro AA (50-100 micrograms/ml) inhibited PO activity of leukocyte lysate and foot extract obtained from untreated animals. In conclusion we found that AA, having no effect on inflammatory response, significantly enhanced inhibition of PO activity in inflamed tissues in mice which could be a result of direct action of AA on the enzyme molecule.
Department of Pneumology and Allergology, Medical Academy, Lódź, Poland.
D
D
Nowak
J
J
Słodkowska
T
T
Pietras
G
G
Piasecka
A
A
Antczak
M
M
Król
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000201:enzymology; D005528:Foot; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009195:Peroxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D012881:Skin Temperature / Q000187:drug effects; D019820:Xanthine; D014969:Xanthine Oxidase; D014970:Xanthines
D014970:Xanthines; D019820:Xanthine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002351:Carrageenan; D009195:Peroxidase; D014969:Xanthine Oxidase; D001205:Ascorbic Acid