Temperature and salt optimization of kappa-carrageenan fractionation by DEAE-cellulose.
Carrageenans play an important role in many medical and food technology applications. However, the study of their properties has been hampered by a lack of well-defined samples (fractions). In this paper, a new principle is presented by which well-defined fractions can be obtained. It is based on batch adsorption to DEAE-cellulose combined with careful control of temperature and salt content. The parameters have been pre-optimized in an analytical column.
Uppsala Universitet, Uppsala Biomedical Center, Sweden.
N
N
Caram-Lelham
R L
RL
Cleland
L O
LO
Sundelöf
D002240:Carbohydrate Sequence; D002351:Carrageenan / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose / Q000379:methods*; D008969:Molecular Sequence Data; D012492:Salts; D013696:Temperature
D012492:Salts; D002351:Carrageenan