[Monoclonal immunophenotyping of acute lymphoblastic leukemia cells in children using rapid immune alkaline phosphatase].
Monoclonal immunophenotyping of leukemia cells of the bone marrow was carried out by the method of rapid immune alkaline phosphatase (RIAP) in 30 patients with acute lymphoblastic leukemia (ALL) aged 6 months-14 years. The authors used a panel of monoclonal antibodies (MCA) produced by Leu (Belgium) and DAKO (Denmark) directed to antigens of differentiation clusters: Tdt, HLA-DR, CD: 10, 19, 20, 22, 7, 8, 2, 5, 13, 33, 14. The results indicate diversity of compositions of differentiating antigens on leukemia cells of the dominant population and a different degree of leukemic cell pool heterogenicity. RIAP advantages over flow cytometry in immunotyping of ALL cells are shown. Arguments are provided in favor of introduction of broader MCA panel.
G V
GV
Kolenkova
A G
AG
Luk'ianova
R V
RV
Lenskaia
N S
NS
Kisliak
M
M
Kenny
T Zh
TZh
Makhartykh
K L
KL
Kondratchik
V M
VM
Chernov
D000469:Alkaline Phosphatase; D000911:Antibodies, Monoclonal; D002648:Child; D002675:Child, Preschool; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000473:pathology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase
Monoklonal'noe immunofenotipirovanie kletok ostrogo limfoblastnogo leĭkoza u deteĭ s pomoshch'iu metoda bystroĭ immunnoĭ shchelochnoĭ fosfatazy.