Asymmetric expression of protein kinase CK2 subunits in human kidney tumors.
Renal clear cell carcinomas and the corresponding ipsilateral control tissues were investigated for protein kinase CK2 activity and subunit ratio. The average protein kinase CK2 activity from 21 different kidney samples was 318 U/mg and that from the corresponding tumors 610 U/mg. The subunit ratio of protein kinase CK2 alpha in tumors/normal tissue (T/N) was 1.58 and that of the protein kinase CK2 beta (T/N) was 2.65. The data suggest that the generally described increase in protein kinase CK2 activity in tumor cells may to some extent result from a deregulation in subunit biosynthesis or degradation. This at least partly owing to the presence of excess enzymatically active protein kinase alpha-subunit but also to a significantly higher presence of the non-catalytic beta-subunit.
Urologische Klinik, Universität des Saarlandes, Homburg/Saar, Germany.
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Stalter
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Siemer
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Becht
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Ziegler
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Remberger
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Issinger
D018262:Adenocarcinoma, Clear Cell / Q000201:enzymology* / Q000473:pathology; D000595:Amino Acid Sequence; D047390:Casein Kinase II; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006801:Humans; D015151:Immunoblotting; D007668:Kidney / Q000201:enzymology*; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000473:pathology; D008163:Luminescent Measurements; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000302:isolation & purification; D013379:Substrate Specificity
D046911:Macromolecular Substances; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases