Photocleavable nitrobenzyl-protein conjugates.
We have developed methods to allow the reversible binding of up to 15 nitrobenzyl residues per bovine serum albumin molecule and show 95% of these residues can be removed by exposure to UV light for 10 min. The general non-specific coating method is presented by means of a model system but is applicable to a wide range of proteins with important biological functions. Potentially, any protein could be coated with sufficient photo-removable groups to inhibit its biological function. Its activity may then be restored at will by exposure to UV light removing the coupled 2-nitrobenzyl groups.
Thompson
S
S
Department of Clinical Biochemistry, Medical School, Newcastle Upon Tyne, United Kingdom.
Spoors
J A
JA
Fawcett
M C
MC
Self
C H
CH
D009578:Nitrobenzenes / Q000737:chemistry*; D010777:Photochemistry; D010782:Photolysis; D011506:Proteins / Q000502:physiology*; D012710:Serum Albumin, Bovine; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D009578:Nitrobenzenes; D011506:Proteins; D012710:Serum Albumin, Bovine