Isolation of pseudorabies (Aujeszky's disease) virus from a Florida panther.
Pseudorabies virus was isolated in cell culture from the brain tissue of a 3.5-year-old male Florida panther (Felis concolor coryi). The virus was not isolated from other tissues collected at necropsy. Based upon a nested polymerase chain reaction (PCR), the virus was determined to have the classical wild-type virulent genotype, glycoprotein I+ (gI+) and thymidine kinase+ (TK+).
Glass
C M
CM
Florida Game and Fresh Water Fish Commission, Wildlife Research Laboratory, Gainesville 32601.
McLean
R G
RG
Katz
J B
JB
Maehr
D S
DS
Cropp
C B
CB
Kirk
L J
LJ
McKeirnan
A J
AJ
Evermann
J F
JF
D000818:Animals; D001921:Brain / Q000382:microbiology*; D002335:Carnivora / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D005838:Genotype; D011558:Herpesvirus 1, Suid / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D009500:Neutralization Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011557:Pseudorabies / Q000382:microbiology*; D013937:Thymidine Kinase / Q000235:genetics; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics
D004279:DNA, Viral; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins; D013937:Thymidine Kinase