Comparison of two monoclonal antibodies, H6B11 and GS2C4, against human plasma gelsolin.
We compared the properties of a new monoclonal antibody developed in our laboratory, H6B11, against human plasma gelsolin with those of GS2C4, which is commercially available. Both antibodies recognize human plasma gelsolin in an enzyme-linked immunosorbent assay (ELISA), and demonstrated similar interference activity for actin gelation, but exhibited differing behaviors toward gel electrophoresis and electroblotting. H6B11 did not bind to gelsolin on an electroblotted membrane after electrophoresis on sodium dodecyl sulfate-polyacrylamide gel, in contrast to GS2C4. However, under certain milder conditions, H6B11 was able to bind to gelsolin. H6B11 was thus judged to be capable of partially recognizing gelsolin conformation, while GS2C4 recognized part of the amino acid-sequence of gelsolin. The faster reaction of H6B11 in the ELISA and the linearity of the results, indicates that the ELISA for plasma gelsolin can be used in routine laboratory testing.
Hiyoshi
M
M
Department of Laboratory Medicine, Osaka City University Medical School, Japan.
Nakao
Y
Y
Tatsumi
N
N
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018260:Gelsolin / Q000032:analysis / Q000097:blood* / Q000302:isolation & purification; D006801:Humans
D000911:Antibodies, Monoclonal; D018260:Gelsolin