Molecular cloning and sequence analysis of the cDNA encoding porcine acrosin inhibitor.
A full-length cDNA encoding the porcine acrosin inhibitor has been isolated from a boar seminal vesicle cDNA library. Nucleotide sequence analysis of the 667-bp cDNA predicts a precursor protein of 97 amino acid residues, which includes a 26-residue signal peptide and a 71-residue secreted protein. The predicted amino acid sequence of the mature protein agrees completely with that of the sperm-associated acrosin inhibitor determined by conventional amino acid sequence analysis. However, the asparagine/aspartic acid and glutamine/glutamic acid substitutions, as reported in the seminal plasma counterpart, have not been observed. Southern blot analysis shows only a single hybridizing band with three different restriction endonucleases, suggesting the presence of a single copy of the acrosin inhibitor gene in the porcine genome.
Department of Obstetrics and Gynecology, Albert Einstein Medical Center, Philadelphia, PA 19141.
S C
SC
Kwok
G
G
Dai
J P
JP
McMurtry
D000176:Acrosin / Q000037:antagonists & inhibitors*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics; D029607:Seminal Vesicle Secretory Proteins; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014018:Tissue Distribution; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D011498:Protein Precursors; D029607:Seminal Vesicle Secretory Proteins; C087994:acrosin inhibitor, porcine; D014359:Trypsin Inhibitor, Kazal Pancreatic; D000176:Acrosin