Markers of coagulation and fibrinolysis in blood drawn into citrate with and without D-Phe-Pro-Arg-Chloromethylketone (PPACK).
In order to compare levels of Prothrombin Fragment 1 + 2 (F1 + 2), Thrombin-antithrombin III complex (TAT), Fibrinogen Degradation Products (FgDP) and Fibrin Degradation Products (FbDP) in plasma from blood drawn into sodium citrate with and without the protease inhibitor D-Phe-Pro-Arg-Chloromethylketone (PPACK), blood samples were collected from 41 patients on the first day after elective gastric surgery. Levels of F1 + 2, TAT and FbDP were not significantly different in plasma with and without PPACK. FgDP levels were significantly higher in plasma with PPACK. Our results did not support previous suggestions that PPACK should be used as a routine anticoagulant for measurement of haemostatic activation products.
Venous Thrombosis Group, Aalborg Hospital, Denmark.
H B
HB
Rahr
J V
JV
Sørensen
D
D
Danielsen
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000990:Antithrombin III / Q000032:analysis; D016477:Artifacts; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation / Q000187:drug effects*; D001800:Blood Specimen Collection / Q000379:methods*; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005342:Fibrinolysis / Q000187:drug effects*; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases / Q000032:analysis; D011184:Postoperative Period; D011516:Prothrombin / Q000032:analysis
D000590:Amino Acid Chloromethyl Ketones; D015415:Biomarkers; D002951:Citrates; D005338:Fibrin Fibrinogen Degradation Products; D010446:Peptide Fragments; C046193:antithrombin III-protease complex; C045879:prothrombin fragment 1.2; D019343:Citric Acid; D000990:Antithrombin III; D011516:Prothrombin; D010447:Peptide Hydrolases; C040308:phenylalanyl-prolyl-arginine-chloromethyl ketone