Differential expression of two testis-specific transcripts of the mouse Pdha-2 gene during spermatogenesis.
Analysis of the expression of the testis-specific isoform of the mouse pyruvate dehydrogenase E1 alpha subunit gene (Pdha-2) during various stages of spermatogenesis has shown that a 2.0-kb Pdha-2 mRNA is initially transcribed in meiotic prophase. The initial appearance of Pdha-2 mRNA precedes that of Pgk-2 and corresponds to the appearance of Ldh-3 mRNA. A second Pdha-2 1.7-kb transcript is present in post-meiotic round spermatids. Polysomal analysis of purified spermatogenic cell populations demonstrates that the 2.0-kb mRNA species is translated in diploid, pachytene spermatocytes and the 1.7-kb mRNA species is translated in round spermatids, although a large proportion is present on the nonpolysomal fraction and may be stored for use in later stages of spermiogenesis.
Department of Anesthetics, University of California, San Francisco 94143.
J
J
Fitzgerald
H H
HH
Dahl
R C
RC
Iannello
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D007527:Isoenzymes / Q000235:genetics*; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D014176:Protein Biosynthesis; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000201:enzymology; D013737:Testis / Q000378:metabolism*
D017931:DNA Primers; D007527:Isoenzymes; D010446:Peptide Fragments; D011768:Pyruvate Dehydrogenase Complex; D012333:RNA, Messenger; D007770:L-Lactate Dehydrogenase; D017853:Phosphotransferases (Alcohol Group Acceptor); C046331:phosphoglucokinase