Mucopolysaccharidosis IV A: molecular cloning of the human N-acetylgalactosamine-6-sulfatase gene (GALNS) and analysis of the 5'-flanking region.
Mucopolysaccharidosis IV A (MPS IV A) is the result of a genetic deficiency in a lysosomal hydrolase, N-acetylgalactosamine-6-sulfatase (GALNS). To investigate MPS IV A patients at the level of the genome, we analyzed the structure of the human GALNS-encoding gene. From the genomic library of a normal subject in lambda EMBL3, we isolated five overlapping clones covering the coding region of the GALNS cDNA and determined the structural organization. The gene is about 50 kb long and contains 14 exons. The 5'-flanking region lacks a canonical TATA box and CCAAT sequences, but is G+C-rich (70.5%), with four GC boxes, characteristic of a housekeeping gene promoter. The transcription initiation site was determined by primer extension analysis, using RNA from human liver and HeLa cells. Transcription was found to initiate at a few sites, the major ones being 58 and 22 bp upstream of the translation initiation codon. The 5'-flanking region had promoter activity by transient expression, determined using a CAT assay. In addition, this region retained promoter activity, even in reverse orientation. The region -98 to -1 upstream of the ATG codon was defined by deletion analysis to be a minimal promoter. One GC box in this region is likely to be a binding site of a regulatory element.
Department of Pediatrics, Gifu University School of Medicine, Japan.
Y
Y
Nakashima
S
S
Tomatsu
T
T
Hori
S
S
Fukuda
K
K
Sukegawa
N
N
Kondo
Y
Y
Suzuki
N
N
Shimozawa
T
T
Orii
D001483:Base Sequence; D002678:Chimera / Q000235:genetics; D002811:Chondroitinsulfatases / Q000172:deficiency / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D015870:Gene Expression; D017930:Genes, Reporter; D015698:Genomic Library; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009085:Mucopolysaccharidosis IV / Q000201:enzymology* / Q000235:genetics*; D009154:Mutation; D011401:Promoter Regions, Genetic; D012326:RNA Splicing / Q000235:genetics
D018076:DNA, Complementary; D002811:Chondroitinsulfatases