Mg+ MNS blood group phenotype: further observations.
Red cells carrying the low-frequency MNS antigen Mg reacted with the only example of anti-DANE, an antibody which had previously defined the GP. Dane (Mi.IX) phenotype. Furthermore, Mg+ cells reacted with the original anti-Mur (serum of Mrs. Murrell), but with none of 14 other anti-Mur. Therefore, Mg+ cells carry both DANE antigen and an atypical Mur antigen. Immunoblotting of membranes from Mg+ cells with anti-M, and with eluates prepared from anti-Mg and Mrs. Murrell's serum demonstrated a glycophorin A (GPA) molecule whose mobility was increased by an apparent M(r) of about 3,000 presumably due to the loss of the three O-glycans known to be absent from Mg-active GPA.
MRC Blood Group Unit, London, UK.
C
C
Green
G
G
Daniels
F
F
Skov
P
P
Tippett
D000595:Amino Acid Sequence; D006801:Humans; D015151:Immunoblotting; D007519:Isoantigens / Q000097:blood* / Q000235:genetics; D008951:MNSs Blood-Group System / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype; D012698:Serologic Tests
D007519:Isoantigens; D008951:MNSs Blood-Group System