Oncostatin M stimulates proliferation in B9 hybridoma cells: potential role of oncostatin M in plasmacytoma development.
Oncostatin M was found to stimulate the IL-6-addicted hybridoma line B9. Leukaemia inhibitory factor did not stimulate proliferation of this line. Both of these factors bind to the gp130 of the IL-6 receptor. In another cell line that is stimulated by LIF (DA.1), neither IL-6 nor oncostatin M stimulated proliferation. Previously it had been thought that the gp130 alone is sufficient to bind ligand and transduce signal and that oncostatin M could bind to and activate the LIF receptor, but these data show this is not always the case. Mice primed with Pristane were found to have IL-6 in their sera and peritoneal fluid only at a few time points following Pristane treatment; this was determined by IL-6-specific ELISA. When the same samples were analysed on IL-6-addicted B9 cells, stimulatory activity was found at all time points. When the Pristane-primed samples were assayed for oncostatin M activity in the A375 melanoma assay, there was oncostatin M activity at various time points. IL-6 did not have activity on the A375 cells. These data indicate that oncostatin M play a role in the generation of plasmacytoma in vivo.
Department of Immunology, Schering-Plough Research Institute, Kenilworth, NJ 07033.
B E
BE
Barton
J V
JV
Jackson
F
F
Lee
J
J
Wagner
D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000494:pharmacology; D006801:Humans; D006825:Hybridomas; D015850:Interleukin-6 / Q000096:biosynthesis / Q000494:pharmacology*; D007700:Kinetics; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology; D008297:Male; D008545:Melanoma; D051379:Mice; D008807:Mice, Inbred BALB C; D053683:Oncostatin M; D010452:Peptide Biosynthesis; D010455:Peptides / Q000494:pharmacology*; D010954:Plasmacytoma / Q000473:pathology*; D013729:Terpenes / Q000633:toxicity; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured
D002273:Carcinogens; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; C508351:OSM protein, human; C508352:Osm protein, mouse; D010455:Peptides; D013729:Terpenes; D053683:Oncostatin M; C009042:pristane