Purification, N-terminal sequence and structural characterization of a toxic protein from the Indian scorpion venom Buthus tamulus.
A toxic protein component (Bt-II) was isolated from the venom of the Indian scorpion, Buthus tamulus, by ion-exchange chromatography on Biorex-70 and reverse phase high pressure liquid chromatography (RP-HPLC). This 7800 mol. wt protein was found to have an LD50 value of 2.25 mg/kg body weight in mice by subcutaneous injection. Amino acid composition of the protein revealed the presence of aromatic and basic amino acids; the N-terminal amino acid sequence was also determined. Structural characterization of Bt-II, based on circular dichroic and fluorescent quenching studies, has also been reported.
Department of Life Sciences, University of Bombay, India.
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Lala
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Narayanan
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D007279:Injections, Subcutaneous; D007928:Lethal Dose 50; D051379:Mice; D008969:Molecular Sequence Data; D012604:Scorpion Venoms / Q000737:chemistry* / Q000302:isolation & purification* / Q000633:toxicity; D012605:Scorpions
D000596:Amino Acids; D012604:Scorpion Venoms