Expression of fos proto-oncogene product by monoclonal antibody FO-120 in smouldering adult T-cell leukaemia (ATL).
We investigated the expression of c-fos gene product in peripheral blood mononuclear cells of patients with smouldering adult T-cell leukaemia (ATL) and healthy human T-lymphotropic virus type-I (HTLV-I) carriers by an immunofluorescence assay, using a mouse monoclonal antibody (FO-120) specific for fos gene product. Peripheral blood mononuclear cells derived from healthy HTLV-I carriers were rarely positive for FO-120, less than 2% of the cells weakly reacted with FO-120, whereas positive cells were detected in more than about 10% of cells from patients with smouldering ATL. FO-120 appears to be a useful tool for detecting smouldering ATL in asymptomatic HTLV-I infected people without using molecular techniques.
Second Department of Internal Medicine, Oita Medical University, Japan.
M
M
Iwahashi
M
M
Otsuka
S
S
Shimotakahara
K
K
Uozumi
S
S
Hanada
T
T
Arima
M
M
Matsumoto
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000097:blood*; D002353:Carrier State / Q000097:blood / Q000175:diagnosis; D003937:Diagnosis, Differential; D005260:Female; D015870:Gene Expression; D015490:HTLV-I Infections / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D015458:Leukemia, T-Cell / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D000090063:Proto-Oncogene Mas; D016760:Proto-Oncogene Proteins c-fos / Q000097:blood*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C000715470:MAS1 protein, human; D009363:Neoplasm Proteins; D000090063:Proto-Oncogene Mas; D016760:Proto-Oncogene Proteins c-fos