DNA sequence and expression analysis of root-knot nematode-elicited giant cell transcripts.
Fifty-eight cDNA clones isolated from a library of transcripts exhibiting up regulation in tomato root giant cells induced by infection with the parasitic nematode Meloidogyne incognita were characterized. A survey of plant tissues identified 31 transcripts present in tissues other than root, including actively dividing and expanding tissues and mature leaf tissues. The identities of approximately 20% of the giant cell transcripts were inferred from DNA sequence data; they include sequences encoding a plasmalemma proton ATPase, a putative Myb-type transcription factor, and the largest subunit of RNA polymerase II.
Department of Nematology, University of California, Riverside 92521.
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D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009349:Nematode Infections / Q000235:genetics; D059828:Plant Cells; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000469:parasitology*; D010947:Plants, Toxic; D012333:RNA, Messenger; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017385:Sequence Homology; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D012333:RNA, Messenger