Increase in stimulatory G protein and glucagon-responsive adenylate cyclase activity in rat liver following partial hepatectomy.
Hepatocytes from partially hepatectomized rat livers show enhanced glucagon-sensitive adenylate cyclase activity. Bmax and Kd values of glucagon receptors (GR), measured by [125I]glucagon binding to liver plasma membranes, remained unchanged following partial hepatectomy. The amount of stimulatory G proteins (Gs), quantified by cholera toxin catalyzed ADP-ribosylation, increased in parallel with an increment in glucagon-responsive adenylate cyclase activity. It is proposed that an increment of Gs is one of the factors responsible for enhancement of glucagon-sensitive adenylate cyclase activity following partial hepatectomy.
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
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Yagami
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002772:Cholera Toxin / Q000737:chemistry; D000242:Cyclic AMP / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005934:Glucagon / Q000378:metabolism* / Q000494:pharmacology; D006498:Hepatectomy; D020120:Iodocyanopindolol; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D018027:Receptors, Glucagon / Q000378:metabolism
D018027:Receptors, Glucagon; D000246:Adenosine Diphosphate Ribose; D020120:Iodocyanopindolol; D005934:Glucagon; D002772:Cholera Toxin; D010869:Pindolol; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D002118:Calcium