Increased secretion of latent elastase activity following contact between human skin fibroblasts and elastin derived peptides.
Elastin-derived peptides were previously shown to influence human skin fibroblasts (HSF) chemotaxis and proliferation (Ghuysen et al., 1992). We report here that culturing HSF on kappa-elastin (kappa E) but not onto fibronectin (FN) enhanced the secretion of latent elastinolytic activity. The proteinase involved was identified as the 72 kDa gelatinase A. Moreover, HSF-kappa E as well as HSF-FN interactions modulated the secretions of Il1 induced expressions of elastinolytic activities.
Biochemistry Department, Faculty of Dental Surgery, University René Descartes Paris V, Montrouge, France.
J M
JM
Landeau
B
B
Pellat
W
W
Hornebeck
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004549:Elastin / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D005353:Fibronectins / Q000378:metabolism*; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000378:metabolism; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D010662:Phenylmercuric Acetate / Q000031:analogs & derivatives; D012867:Skin / Q000166:cytology / Q000201:enzymology*; D013439:Sulfhydryl Reagents
D016895:Culture Media, Serum-Free; D005353:Fibronectins; D007375:Interleukin-1; D010446:Peptide Fragments; D013439:Sulfhydryl Reagents; C010024:4-aminophenylmercuriacetate; D004549:Elastin; D010450:Endopeptidases; D010196:Pancreatic Elastase; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D010662:Phenylmercuric Acetate