Cyto- and genotoxic potential of the photosensitizer Photosan 3 in the absence of light.
In the present investigation, cultures of rat hepatocytes were treated with Photosan 3 (PS 3) and examined for possible mutagenic effects in the absence of light. Hepatocytes are particularly useful in genotoxicity studies because of their capacity to metabolize a large variety of promutagenic/procarcinogenic compounds. The cytogenetic endpoints determined were chromosomal aberrations and the induction of micronuclei. Three hours of incubation with PS 3 (at concentrations of 0.1, 1, 10, and 100 micrograms ml-1) induced significantly elevated levels of chromosomal aberrations and micronuclei already at the lowest concentration of 0.1 micrograms ml-1 compared with the controls. A concentration of 100 micrograms ml-1 PS 3 appeared to be cytotoxic: no mitotic figures could be detected.
Division of Physics and Biophysics, University of Salzburg, Austria.
D M
DM
Fiedler
B
B
Krammer
P M
PM
Eckl
D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D005260:Female; D006415:Hematoporphyrins; D008027:Light; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D017319:Photosensitizing Agents / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344
D006415:Hematoporphyrins; D009153:Mutagens; D017319:Photosensitizing Agents; C071875:photosan III