Pertussis toxin activates platelets through an interaction with platelet glycoprotein Ib.
Platelets present a unique model to study the B-oligomer effects of pertussis toxin because they become activated in response to the B oligomer but are not susceptible to ADP-ribosylation by the holotoxin. In these studies, the B oligomer of pertussis toxin caused concentration-dependent platelet activation, as determined by increases in intracellular calcium concentration, dense granule secretion, and platelet aggregation. Stirring was required for pertussis toxin to increase intracellular calcium. A monoclonal antibody against platelet glycoprotein Ib abolished increases in intracellular calcium concentration and increased the latency and reduced the slope of the aggregation response elicited by the B oligomer. Pertussis toxin also evoked [14C]serotonin release from platelets, and this effect was inhibited, though not eliminated, by an antibody against platelet glycoprotein Ib. Binding of pertussis toxin to glycoprotein Ib was observed after nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These data suggest that the B oligomer of pertussis toxin induces platelet activation mediated, at least in part, by an interaction with platelet glycoprotein Ib.
Sindt
K A
KA
Department of Pharmacology, University of Virginia, Charlottesville 22908.
Hewlett
E L
EL
Redpath
G T
GT
Rappuoli
R
R
Gray
L S
LS
Vandenberg
S R
SR
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D006801:Humans; D037342:Pertussis Toxin; D015539:Platelet Activation / Q000187:drug effects*; D010980:Platelet Membrane Glycoproteins / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D010980:Platelet Membrane Glycoproteins; D010566:Virulence Factors, Bordetella; D012701:Serotonin; D037342:Pertussis Toxin; D002118:Calcium