Cytogenetic analysis of murine cell lines from diethylstilbestrol-induced uterine endometrial adenocarcinomas.
Treatment of female CD-1 mice with the synthetic estrogen diethylstilbestrol (DES) on days 1 through 5 after birth results in a 90% incidence of endometrial adenocarcinomas by 18 months of age Three cell lines were established from DES-induced uterine carcinomas and studied for specific chromosomal changes. Each cell line exhibited numerical decreases in chromosomes 9, 11, 13, and X as common abnormalities. Structural alterations involving chromosomes 3, 6, 11, and 19 occurred nonrandomly among the three cell lines. Every cell line showed a rearrangement in the long arm of chromosome 3 (3q+), a translocation between chromosomes 3 and 19 [t(3;19)], isochromosome of chromosome 11 [i(11)], and a marker chromosome (M2) either as common abnormalities or recurrent abnormalities. t(3;19), i(11), and M2 were observed also in the primary colonies from which the cell lines arose. The changes were not observed in a cell line derived from the uterus of one untreated control mouse, suggesting that these chromosomal alterations may have occurred during DES-induced neoplastic transformation. The chromosomal alterations found in the present study may prove useful in investigating the genetic changes involved in DES carcinogenesis.
Endo
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Laboratory of Reproductive and Development Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
Kodama
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Newbold
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McLachlan
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Barrett
J C
JC
D000230:Adenocarcinoma / Q000139:chemically induced* / Q000235:genetics*; D000782:Aneuploidy; D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002869:Chromosome Aberrations; D004054:Diethylstilbestrol / Q000009:adverse effects*; D005260:Female; D007621:Karyotyping; D051379:Mice; D014178:Translocation, Genetic; D014594:Uterine Neoplasms / Q000139:chemically induced* / Q000235:genetics*
D004054:Diethylstilbestrol