Determination of 18 beta-glycyrrhetic acid in human plasma by high-performance liquid chromatography.
A sensitive high-performance liquid chromatographic method has been developed for the determination of 18 beta-Glycyrrhetic acid in human plasma. 18 alpha-Glycyrrhetic acid was used as an internal standard. The procedure includes pretreatment by liquid-liquid extraction and chromatographic separation on a reversed-phase column with ultraviolet detection. The detection limit of the drug was 5 ng/ml. The method was applied to the determination of 18 beta-glycyrrhetic acid in plasma after oral administration of a herbal medicine containing 18 beta-glycyrrhitic acid in human volunteers.
Abe
K
K
Traditional Chinese Medicine Research Labs., Kanebo Ltd., Osaka, Japan.
Suzuki
A
A
Katayama
H
H
Tatsumi
Y
Y
Yumioka
E
E
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics; D006034:Glycyrrhetinic Acid / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; C018142:saiko-keishi-to; D006034:Glycyrrhetinic Acid