Analysis of cocaine and metabolites in brain using solid phase extraction and full-scanning gas chromatography/ion trap mass spectrometry.
A method is described for the determination of cocaine, benzoylecgonine and cocaethylene in the human brain using Clean Screen solid phase extraction cartridges and gas chromatography/ion trap mass spectrometry with electron impact and full scan analysis. The procedure uses deuterated internal standards. Run-to-run and within-run coefficients of variation are < 7% and the sensitivity proved to be 50 ng/g from 1 g of sample. The procedure has been applied to a number of forensic cases involving cocaine intoxication. Cocaine was relatively unstable in brain tissue stored at 4 degrees C when compared to storage at -80 degrees C.
Dade County Medical Examiner Department, Miami, Florida 33136-1133.
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Hernandez
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Andollo
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Hearn
D001923:Brain Chemistry; D002423:Cause of Death; D003042:Cocaine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D009294:Narcotics / Q000032:analysis* / Q000097:blood; D014179:Neurotransmitter Uptake Inhibitors / Q000032:analysis* / Q000097:blood
D009294:Narcotics; D014179:Neurotransmitter Uptake Inhibitors; C005618:benzoylecgonine; C066444:cocaethylene; D003042:Cocaine