Cryopreservation of Babesia caballi cultures.
Babesia caballi cultures were cryopreserved with a solution of 10% (w/v) polyvinylpyrrolidone 40 as cryoprotectant. Samples were cooled at rates of 1, 10, 30 and 100 degrees C min-1 using a programmable freezer. Additionally, a styrofoam box designed to cool samples at an approximate rate of 10 degrees C min-1 when placed in a -80 degrees C freezer was used. Samples were stored in liquid nitrogen, thawed rapidly and inoculated into cultures. Although, a high loss of infectivity was observed after cryopreservation, cultures could be initiated reliably from cryo-stabilates frozen at a rate of 10 and 30 degrees C min-1 or frozen with the styrofoam box.
Institute of Parasitology, School of Veterinary Medicine, Bünteweg, Hannover, Germany.
B
B
Hentrich
R
R
Böse
M
M
Doherr
D000818:Animals; D001403:Babesia / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary*; D004912:Erythrocytes / Q000469:parasitology*; D006736:Horses; D011205:Povidone
D011205:Povidone