Brain-derived neurotrophic factor and trkB receptor mRNAs in grafts of cortex cerebri.
Trophic factors are expressed by neurons throughout several areas of the CNS. We studied the mRNA expression of a member of the neurotrophin family, brain-derived neurotrophic factor (BDNF), and of the two receptor transcripts, full-length trkB and truncated trkB in single intraocular cortex cerebri grafts and in double cortex cerebri grafts. All single as well as double intraocular cortex grafts grew well, reaching a maximal size 4 weeks postgrafting. BDNF mRNA was moderately expressed in neurons in all intraocular grafts and significantly increased compared to that in adult rat cortex. Truncated trkB mRNA was strongly expressed in neurons and glia, while full-length trkB mRNA was moderately expressed only in neurons in the intraocular cortical grafts. The expression of the two trkB transcripts in the grafted cortex did not differ from that of adult rat cortex. No difference in the expression of mRNAs for full-length or truncated trkB was found between single grafts grown for 4 or 8 weeks or between single and double grafts. Similarly, no difference in expression of BDNF mRNA in single grafts grown for 4 or 8 weeks was detected. However, BDNF mRNA levels were significantly lower in grafts which were placed in close contact with previously grafted cortex in the eye chamber. Moreover, contact with a second graft led to downregulation of BDNF mRNA in the first graft.(ABSTRACT TRUNCATED AT 250 WORDS)
Humpel
C
C
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Giacobini
M M
MM
Wetmore
C
C
Olson
L
L
D000818:Animals; D016380:Brain Tissue Transplantation; D019208:Brain-Derived Neurotrophic Factor; D002540:Cerebral Cortex / Q000378:metabolism* / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009799:Ocular Physiological Phenomena; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor / Q000235:genetics*
D019208:Brain-Derived Neurotrophic Factor; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor