A human promyelocyte mRNA transiently induced by TPA is homologous to yeast IPP isomerase.
A cDNA clone was isolated from HL60 human promyelocyte cells on the basis of the induction of its cognate mRNA by the phorbol ester TPA (12-O-tetradecanoyl phorbol-13-acetate) in the presence of cycloheximide. Northern analysis showed a peak of induction of a 1.9-kb mRNA at 3-6 h after TPA addition. Sequence analysis revealed that this cDNA was likely a human homolog of yeast isopentenyl diphosphate:dimethylallyl diphosphate isomerase, an enzyme important for isoprenoid synthesis and the prenylation of proteins such as ras p21.
Cancer Research Laboratory, University of Western Ontario, London, Canada.
J W
JW
Xuan
J
J
Kowalski
A F
AF
Chambers
D T
DT
Denhardt
D000595:Amino Acid Sequence; D001483:Base Sequence; D019748:Carbon-Carbon Double Bond Isomerases; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism*; D045782:Hemiterpenes; D006801:Humans; D007535:Isomerases / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D018076:DNA, Complementary; D045782:Hemiterpenes; D012333:RNA, Messenger; D007535:Isomerases; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase; D013755:Tetradecanoylphorbol Acetate