Cross-reactivity of ovine IgE with anti-human IgE.
Cross-reactivity between ovine and human IgE was investigated using monoclonal antibodies to human IgE heavy chain. Western immunoblotting, reverse cutaneous anaphylaxis (RCA), passive cutaneous anaphylaxis inhibition (PCAI) and reverse passive cutaneous anaphylaxis (RPCA) tests were all used to assess cross-reactivity between the monoclonals and ovine IgE. No cross-reactivity was demonstrated using Western immunoblotting, RCA and PCAI tests. However, evidence for homology in the region of the molecule concerned with mast cell binding was demonstrated by RPCA performed in sheep skin primed with human myeloma IgE.
School of Veterinary Science, Department of Veterinary Medicine, Bristol, UK.
H
H
Yilmaz
K L
KL
Morgan
J M
JM
Roe
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D003429:Cross Reactions / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology; D012756:Sheep / Q000276:immunology*; D012882:Skin Tests
D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D007073:Immunoglobulin E