Radiation-induction of micronuclei in human peripheral blood lymphocytes: effect of freezing.
Lymphocytes in peripheral blood samples from three donors were irradiated at doses of 0, 1 and 2 Gy with cobalt-60 gamma rays. Whole blood cultures were established immediately after irradiation. Cultures of Ficoll-Paque separated lymphocytes were set up immediately after irradiation, or 3 weeks after storage in liquid nitrogen at -196 degrees C. The frequency of micronuclei was measured in binucleate cytokinesis-blocked cells and the different treatment groups were compared. No clastogenic effect of the lymphocyte separation technique, or of subsequent freezing in liquid nitrogen, was observed.
Biomedicine and Health Program, Australian Nuclear Science and Technology Organisation, Menai, NSW.
J S
JS
Prosser
B E
BE
Izard
M A
MA
Wallace
D000328:Adult; D002467:Cell Nucleus / Q000528:radiation effects / Q000648:ultrastructure; D002478:Cells, Cultured; D015925:Cryopreservation; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects* / Q000648:ultrastructure*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects* / Q000648:ultrastructure*; D013997:Time Factors