Plasmids in several strains of Deinococcus radiodurans.
Deinococcus radiodurans is known as a radioresistant bacterium. For the construction of the shuttle vector, plasmids of this bacterium were isolated and investigated. At least two new plasmids in the cells of D. radiodurans MR1, KR1 and Sark were found in this experiment. However, a common plasmid among MR1, KR1 and Sark strains could not be found. These plasmids would be useful for the construction of the shuttle vector for cloning radioresistant genes of this bacterium.
Biotechnology Laboratory, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, Gunma.
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Kikuchi
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Kitayama
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Sjarief
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Watanabe
D004269:DNA, Bacterial / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005822:Genetic Vectors; D006095:Gram-Positive Cocci / Q000235:genetics*; D008970:Molecular Weight; D010957:Plasmids / Q000302:isolation & purification; D015183:Restriction Mapping; D013045:Species Specificity
D004269:DNA, Bacterial