Hydrolytic enzymes and lectin-binding activity of black-pigmented anaerobic rods.
Recent taxonomic studies on black-pigmented anaerobic rods, a group of bacteria found on mucosal surfaces of humans and animals, led to the subdivision of existing species and to the creation of new species. The aim of this study was to characterize all 11 currently recognized species of black-pigmented bacteria (55 strains) for their ability to hydrolyse a variety of natural and synthetic substrates and for their lectin reactivity. Although most of the strains demonstrated some activity against proteinaceous substrates, Porphyromonas gingivalis was the only species able to hydrolyse type I collagen. Most strains possessed glycylprolyl protease activity, elastase-like activity and phospholipase C activity, whereas trypsin-like activity was restricted to P. gingivalis, Porphyromonas salivosa and Bacteroides macacae. beta-Lactamase activity was demonstrated in five strains belonging to the saccharolytic group. The lectin reactivity of the bacteria was determined by a dot-blot procedure using horseradish-peroxidase-conjugated lectins. Three lectins, LOTUS A, RCA-I and ConA, failed to react with any of the bacteria tested. WGA reacted strongly with the cell surface of human biotypes of asaccharolytic black-pigmented bacteria (P. gingivalis, Porphyromonas asaccharolytica and Porphyromonas endodontalis) and Prevotella intermedia. The animal biotype strains of P. gingivalis showed a higher affinity for SBA and PNA than for WGA.
Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, Canada.
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Grenier
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Labbé
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Mouton
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Meyrand
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis*; D001439:Bacteroides / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D017364:Collagenases / Q000032:analysis; D010450:Endopeptidases / Q000032:analysis; D006089:Gram-Negative Anaerobic Bacteria / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D006867:Hydrolases / Q000032:analysis*; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology; D010858:Pigmentation; D017861:Porphyromonas / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D013045:Species Specificity; D013379:Substrate Specificity; D010738:Type C Phospholipases / Q000032:analysis; D001618:beta-Lactamases / Q000032:analysis
D001426:Bacterial Proteins; D037102:Lectins; D006867:Hydrolases; D010738:Type C Phospholipases; D010450:Endopeptidases; D017364:Collagenases; D001618:beta-Lactamases