The effect of anti-exotoxin A on the adherence of Pseudomonas aeruginosa to hamster tracheal epithelial cells in vitro.
One of the most important initial events of colonization and infection of epithelial tissues is the adherence of bacteria to mucosal surfaces. Bacterial adhesion to the epithelial cell may be mediated by a variety of adhesins, including exoproducts. One of these exoproducts, exotoxin A (EA) is a three-domain bacterial toxin that kills mammalian cells by gaining entry to the cytosol and inactivating protein synthesis. In the present study, HTE cultures, 2-4 weeks in vitro (containing both ciliated and non-ciliated cells), were treated for 1 hr with two different non-mucoid strains of Pseudomonas aeruginosa (1 x 10(8) organisms/ml) in the presence of anti-EA. 50 randomly selected fields were evaluated via SEM at x2500 magnification and the number of bacterial clusters/field quantitated. The results of this study indicate, first, that both piliated (ATCC15692) and non-piliated (PAKp) P. aeruginosa will bind to the HTE cells and, second, that treatment of HTE cells with either strain of P. aeruginosa in the presence of anti-EA will reduce bacterial binding by 25% to 50%. Thus, EA may participate in the adhesion of P. aeruginosa to respiratory tract epithelia.
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.
P C
PC
Moller
M J
MJ
Evans
R C
RC
Fader
L C
LC
Henson
B
B
Rogers
J P
JP
Heggers
D036002:ADP Ribose Transferases; D000818:Animals; D000911:Antibodies, Monoclonal; D001422:Bacterial Adhesion / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D002478:Cells, Cultured; D006224:Cricetinae; D004847:Epithelial Cells; D005098:Exotoxins / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D014132:Trachea / Q000166:cytology*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D005098:Exotoxins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A