Microdialysis study of ischemia-induced hydroxyl radicals in the canine heart.
A new experimental approach for spin-trapping of oxygen radicals in a selected region of the heart in situ is described. This approach is based on microdialysis, and it permits the detection of oxygen radicals in conditions of local ischemia and restoration of normal blood flow. Increased hydroxyl radical generation in an ischemic area of canine myocardium, as a result of 40 min local occlusion, has been studied.
Timoshin
A A
AA
Institute of Experimental Cardiology, Russian Academy of Medical Sciences, Moscow.
Tskitishvili
O V
OV
Serebryakova
L I
LI
Kuzmin
A I
AI
Medvedev
O S
OS
Ruuge
E K
EK
D000818:Animals; D003497:Cyclic N-Oxides; D004285:Dogs; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D017665:Hydroxyl Radical / Q000032:analysis* / Q000378:metabolism; D008297:Male; D017551:Microdialysis / Q000379:methods*; D017202:Myocardial Ischemia / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D013113:Spin Labels; D017665:Hydroxyl Radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide