[Chemiluminescent determination of superoxide dismutase activity].
A specific chemiluminescent method for the assessment of superoxide dismutase (SOD) activity in human blood has been developed. Intensive lucinogen-dependent chemiluminescence was observed during adrenalin oxidation in alkaline medium, and the level of this chemiluminescence essentially decreased in the presence of SOD. 50% inhibition of chemiluminescence was observed in the presence of 25 ng/ml of the enzyme, this indicating a high sensitivity of the method as against a similar spectrophotometric technique. SOD activities in donor blood samples were measured. The described method is offered for clinical application.
Z P
ZP
Cheremisina
T B
TB
Suslova
L G
LG
Korkina
D006801:Humans; D008163:Luminescent Measurements; D013482:Superoxide Dismutase / Q000097:blood*
D013482:Superoxide Dismutase
Khemiliuminestsentnoe opredelenie aktivnosti superoksiddismutazy.