Mutations in the tetA(B) gene that cause a change in substrate specificity of the tetracycline efflux pump.
The tetA(B) gene from transposon Tn10 fails to mediate resistance to the novel tetracycline analog 9-(dimethylglycylamido)minocycline (DMG-Mino) (P. E. Sum, V. J. Lee, R. T. Testa, J. J. Hlavka, G. A. Ellestad, J. D. Bloom, Y. Gluzman, and F. P. Tally, J. Med. Chem. 37:184-188, 1994; R. T. Testa, P. Petersen, N. V. Jacobus, P. E. Sum, V. J. Lee, and F. P. Tally, Antimicrob. Agents Chemother. 37:2270-2277, 1993). Mutations in either of two codons of tetA(B) that resulted in increased resistance to DMG-Mino also caused diminished resistance to tetracycline, identifying amino acid residues critical for the recognition of tetracycline.
Department of Microbial Genetics, Lederle Laboratories, Pearl River, New York 10965.
G G
GG
Guay
M
M
Tuckman
D M
DM
Rothstein
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001693:Biological Transport, Active; D008566:Membranes / Q000378:metabolism; D008911:Minocycline / Q000494:pharmacology; D008969:Molecular Sequence Data; D010957:Plasmids; D017433:Protein Structure, Secondary; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013752:Tetracycline / Q000378:metabolism*; D013753:Tetracycline Resistance / Q000235:genetics*
D001426:Bacterial Proteins; D013752:Tetracycline; D008911:Minocycline