Down-regulation of the human c-fos and c-myc proto-oncogene promoters by adeno-associated virus Rep78.
Adeno-associated virus (AAV) is a non-pathogenic human parvovirus which has anti-tumor and anti-proliferation properties in tissue culture and animal studies. Furthermore, AAV infection is negatively associated with human cervical cancer. C-myc has been implicated in cervical cancer, and c-fos is involved in signal transduction initiation of cell growth. To study the potential regulation of these two prominent human proto-oncogenes by AAV, the expression of three marker coding sequences ligated 3' of the proto-oncogene promoters were observed. Demonstrated here, the AAV Rep78 gene product was able to down-regulate the human c-fos and c-myc proto-oncogene promoters in all three assay systems. These interactions may partially explain AAVs anti-proliferation properties.
University of Arkansas for Medical Sciences, Department of Obstetrics and Gynecology, Little Rock 72205.
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D000818:Animals; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics*; D000229:Dependovirus / Q000235:genetics*; D015536:Down-Regulation; D016762:Genes, fos; D016259:Genes, myc; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D012091:Repetitive Sequences, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics*
D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D014764:Viral Proteins; C068734:rep proteins, Adeno-associated virus 2