GATA-1, GATA-2, and stem cell leukemia gene expression in acute myeloid leukemia.
Transcription factors play an important role in the normal developmental process of hematopoietic cells. However, expression of transcription factors and its implication in various human leukemias is not well understood. We have studied GATA-1, GATA-2, and stem cell leukemia (SCL) gene expression in 30 patients with acute myeloid leukemia (AML) by the reverse transcription-polymerase chain reaction assay. In AML both GATA-1 and SCL genes were commonly expressed in M6 and M7 leukemias, and also in leukemias bearing the platelet-associated antigen. We found some AML patients with GATA-1, but not SCL expression. Most CD7+ AML and t(8;21)(q22;q22)-AML were included in this group, which often demonstrated immunoglobulin heavy chain and/or T-cell receptor gene rearrangements. Consequently, GATA-1+ SCL- AML may originate from early myeloid progenitors. Moreover, most AML patients of the M3, M4, or M5 groups were GATA-1- SCL-. Our data suggest that the expression pattern of transcription factors may help to define distinct phenotypes of AML cells.
First Department of Internal Medicine, Tokyo Medical College, Japan.
T
T
Shimamoto
J H
JH
Ohyashiki
K
K
Ohyashiki
K
K
Kawakubo
N
N
Kimura
S
S
Nakazawa
K
K
Toyama
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D050981:Erythroid-Specific DNA-Binding Factors; D005260:Female; D050982:GATA1 Transcription Factor; D050989:GATA2 Transcription Factor; D015870:Gene Expression; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D050989:GATA2 Transcription Factor; C494711:GATA2 protein, human; D014157:Transcription Factors