[Detection of anti-neutrophil antibodies by enzyme linked immunosorbent assay--comparison of results obtained by ELISA with those by leukocyte agglutination test and granulocyte cytotoxicity test].
We examined anti-neutrophil antibodies in 12 patients with various disorders using enzyme-linked immunosorbent assay (ELISA), and the results obtained by ELISA were compared with those obtained by leukocyte agglutination test (LAT) and granulocyte cytotoxicity test (GCT). IgG anti-neutrophil antibody was positive in 7 of 12 patients, and IgM type antibody was positive in 6 patients. There was a significant correlation between IgG and IgM anti-neutrophil antibodies. The results obtained by ELISA were not in accord with those obtained by LAT or GCT. In addition, we examined anti-neutrophil antibodies in serially collected serum samples from two patients with immune neutropenia. The results obtained by ELISA correlated with their neutrophil counts, suggesting that anti-neutrophil antibodies detected by ELISA have pathological relevance.
Hayashi
S
S
Department of Blood Transfusion, Osaka University Hospital, Suita.
Kiyokawa
T
T
Aochi
H
H
Nagamine
K
K
Oshida
M
M
Tomiyama
Y
Y
Kurata
Y
Y
D000328:Adult; D000368:Aged; D000372:Agglutination Tests; D001323:Autoantibodies / Q000032:analysis*; D003601:Cytotoxicity Tests, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006098:Granulocytes; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007962:Leukocytes; D008297:Male; D009504:Neutrophils / Q000276:immunology*
D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M