Platelet-activating factor antagonists and implantation in rabbits.
In an initial experiment, rabbits were injected i.v. with a platelet-activating factor (PAF) antagonist CV-3988 twice a day on days 5 and 6 of pregnancy. Some inhibition of implantation was observed. This effect could not be reproduced in subsequent experiments at the same or at larger or smaller doses. The non-metabolized analogue of PAF, N-carbamyl-PAF (C-PAF) had an inhibitory effect on implantation only when given at toxic concentrations. When CV-3988 and C-PAF were given together on days 5 and 6, there was no effect on implantation. None of the other PAF antagonists tested--BN52021, SRI63,441, WEB2086 or TCV-309--at various doses could inhibit implantation when given on the same days of pregnancy. TCV-309, at 0.1 mg kg-1 i.v. given on days 2-4 of pregnancy, was also ineffective. These results provide no clear support for a role of PAF in implantation in rabbits.
Norris
C J
CJ
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio 78284-7836.
Peairs
W A
WA
Kudolo
G B
GB
Newton
E R
ER
Harper
M J
MJ
D000818:Animals; D004334:Drug Administration Schedule; D010064:Embryo Implantation / Q000187:drug effects*; D005260:Female; D010742:Phospholipid Ethers / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D011247:Pregnancy; D011817:Rabbits
D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; C053479:1-O-hexadecyl-2-N-methylcarbamylphosphatidylcholine; C037913:CV 3988