Peroxisomal acyl-coenzyme A oxidase is a rate-limiting enzyme in a very-long-chain fatty acid beta-oxidation system.
The contents of peroxisomal fatty acid beta-oxidation enzymes in three rat hepatoma cell lines, i.e., H4IIEC3 (H4), N1S1, and McA-RH7777 (H7), were measured by immunoblot analysis, and a significant difference in acyl-coenzyme A oxidase (AOX) content became evident. These cell lines were respectively infected with a recombinant virus to express significant amounts of AOX protein. The expressed AOX mainly localized in organelle, supposing peroxisomes, and was catalytically active. The cDNA-expression in H4, N1S1, and H7 cells enhanced 2.6-, 2.2-, and 1.0-fold beta-oxidation activity of lignoceric acid, respectively. The enhancement in H4 and N1S1 cells suggests that AOX is a rate-limiting enzyme in the very-long-chain fatty acid beta-oxidation system, in these cell lines.
Aoyama
T
T
Department of Biochemistry, Shinshu University School of Medicine, Nagano, Japan.
Souri
M
M
Kamijo
T
T
Ushikubo
S
S
Hashimoto
T
T
D042962:Acyl-CoA Oxidase; D000818:Animals; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005227:Fatty Acids / Q000378:metabolism*; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D008830:Microbodies / Q000201:enzymology*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D051381:Rats
D018076:DNA, Complementary; D005227:Fatty Acids; D010171:Palmitoyl Coenzyme A; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase; C010210:lignoceric acid