[A new site-specific endonuclease BspKT51].
The new site-specific endonuclease BspKT5I free from interfering impurities has been isolated from thermophilic soil bacteria Bacillus species KT5 by successive chromatography on blue agarose, hydroxyapatite and heparin-Sepharose. BspKT5I on double-stranded DNA recognizes the sequence 5'-CTGAAG16N decreases 3'-GACTTC14N increases and cleaves the DNA at the recognition site as indicated by the arrows to form dinucleotide 3'-protruding termini. The isolated endonuclease is an isoschisomer of Eco57I. However, unlike Eco57I, it is not stimulated by S-adenosylmethionine (SAM) and can therefore be related to subclass IIs but not to IV, as Eco57I. In addition, endonuclease BspKT5I, unlike Eco57I, has no methylase activity.
N I
NI
Shapovalova
L A
LA
Zheleznaia
N N
NN
Matvienko
N I
NI
Matvienko
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D002850:Chromatography, Gel; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D004247:DNA; D004262:DNA Restriction Enzymes; C054219:endodeoxyribonuclease Eco57I
Novaia saĭt-spetsificheskaia éndonukleaza BspKT51.