Specificity and sequence requirements for interactions between various retroviral Gag proteins.
We previously established a genetic assay for retroviral Gag polyprotein multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S. P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to demonstrate homomeric interactions between Gag polyproteins encoded by six different retroviruses. Of the Gag polyproteins tested, only those encoded by closely related retroviruses formed heteromultimers. To determine the primary sequence requirements for human immunodeficiency virus type 1 Gag polyprotein multimerization, we studied the effects on multimerization of deletion and linker insertion mutations. Sequences necessary for this process were located between the C-terminal one-third of the capsid domain and the C terminus of the nucleocapsid domain.
Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
E K
EK
Franke
H E
HE
Yuan
K L
KL
Bossolt
S P
SP
Goff
J
J
Luban
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D005821:Genetic Techniques; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012190:Retroviridae / Q000235:genetics / Q000378:metabolism*
D004279:DNA, Viral; D015683:Gene Products, gag