Mechanical loading triggers specific biochemical responses in mandibular condylar chondrocytes.
The effect of mechanical loading on the phosphorylation state of condylar cartilage proteins was investigated by high resolution electrophoretic analysis of 32P-labelled proteins from mechanically stimulated rat mandibular condylar chondrocytes. Specific dephosphorylation (and/or loss) of an acidic, 35-36 kDa protein(s) and of proteins overlapping with members of the ras superfamily of small GTP-binding proteins was observed. These responses may constitute part of a mechanically induced transduction system which establishes the differentiated phenotype.
Poliklinik für Kieferorthopädie, Universität Heidelberg, Germany.
E K
EK
Basdra
L A
LA
Huber
G
G
Komposch
A G
AG
Papavassiliou
D016475:3T3 Cells; D000818:Animals; D002356:Cartilage / Q000378:metabolism; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D019204:GTP-Binding Proteins / Q000737:chemistry; D007525:Isoelectric Focusing; D008334:Mandible / Q000378:metabolism*; D051379:Mice; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D013314:Stress, Mechanical
D011506:Proteins; D019204:GTP-Binding Proteins