Evaluation of a kinetic enzyme-linked immunosorbent assay for detection of caprine arthritis-encephalitis virus-specific antibodies.
A kinetic indirect enzyme-linked immunosorbent assay (k-ELISA) was evaluated for detection of antibody to caprine arthritis-encephalitis virus (CAEV), using sodium dodecyl sulfate-treated CAEV-63 as antigen. Two hundred fifteen caprine sera submitted to the diagnostic laboratory were tested for CAEV antibody by the k-ELISA and by immunoprecipitation of [35S]-methionine-labeled CAEV. A k-ELISA positive cutoff point of 80 yielded a sensitivity of 94.4% and a specificity of 100%, as compared with immunoprecipitation. A k-ELISA cutoff point of 50 resulted in a sensitivity of 100%, with 95.6% specificity. When sera with k-ELISA scores between 50 and 80 were considered suspect, testing of 1,001 diagnostic sera resulted in < 1.5% suspect reactions. Using the 80 cutoff point, the CAEV k-ELISA had good sensitivity and specificity, with the added advantages of quick turn-around time, few suspect reactions, and adaptability to large numbers of samples
Washington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Washington State University, Pullman 99165.
J
J
Vander Schalie
D S
DS
Bradway
T E
TE
Besser
J F
JF
Evermann
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D016088:Arthritis-Encephalitis Virus, Caprine / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015511:Goat Diseases; D006041:Goats; D007700:Kinetics; D016180:Lentivirus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D008715:Methionine / Q000378:metabolism; D011233:Precipitin Tests / Q000379:methods; D012680:Sensitivity and Specificity; D013462:Sulfur Radioisotopes
D000914:Antibodies, Viral; D013462:Sulfur Radioisotopes; D008715:Methionine