32P-post-labeling detection of DNA adducts in mononuclear cells of workers occupationally exposed to styrene.
32P-Post-labeling was used to analyze for the presence of DNA adducts in 47 workers exposed to styrene in a boat manufacturing facility. Individual airborne exposures measured several times over the course of 1 year ranged from 1 to 235 mg/m3 with a mean value of 65.6 mg/m3. Two adducts were detected in the DNA of mononuclear cells of these workers. The following levels of adducts were detected: adduct 1, range 0.6-102 x 10(-8) (mean 15.8 x 10(-8); adduct 2, range 0.1-70.9 x 10(-8) (mean 14.2 x 10(-8). Significant linear relationships were found between styrene exposure and both DNA adducts (adduct 2, r = 0.330, P = 0.012; adduct 1, r = 0.244, P = 0.049). Co-chromatography experiments identified DNA adduct 1 in the exposed samples as N2-(2-hydroxy-1-phenylethyl)-2'-deoxyguanosine-3',5'-bisphosphate. DNA adduct 2 remains unidentified. No significant linear relationships were observed between the level of DNA adducts and sister chromatid exchanges, possibly because of the poor precision of the 32P-post-labeling assay (the estimated coefficients of variation for adducts 1 and 2 were 2.54 and 1.96, respectively). These results demonstrate that occupational exposure to styrene results in the formation of DNA adducts in human mononuclear cells.
Department of Neurological Surgery, University of California, San Francisco 94143-0806.
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Horvath
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Pongracz
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Rappaport
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WJ
Bodell
D004247:DNA / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects*; D010761:Phosphorus Radioisotopes; D012854:Sister Chromatid Exchange / Q000187:drug effects; D020058:Styrene; D013343:Styrenes / Q000009:adverse effects*
D004852:Epoxy Compounds; D010761:Phosphorus Radioisotopes; D013343:Styrenes; D020058:Styrene; D004247:DNA; C013690:styrene oxide