Effect of aspirin on beta-receptors in lymphocytes from patients with aspirin-induced asthma.
We studied the effect of aspirin on beta-adrenergic receptors in lymphocytes from three groups of subjects: 5 asthmatic patients presenting sensitivity to aspirin, 10 asthmatic patients with tolerance to aspirin and a control group. Lymphocytes were incubated with aspirin-lysine (36 micrograms/ml). The number of beta-receptors/cell and the dissociation constants (Kd) were assessed by means of [125I]-cyanopindolol (ICYP). There was a decrease in the number of beta-receptors after incubation with aspirin in all asthmatic patients with sensitivity. The differences between mean basal (660 +/- 171 receptors/cell) and postincubation (398 +/- 110 receptors/cell) values were significant (p < 0.05). There were also significant differences (p < 0.01) between basal (23.4 +/- 6 pM) and postincubation (15 +/- 5.1 pM) Kd values, which indicates that the affinity of ICYP increased. In the subjects in the other two groups, aspirin both increased and decreased the number of beta-receptors, but the effects were not significant. It is suggested that in patients with sensitivity, aspirin could induce changes in the plasmatic membrane, thus altering beta-receptors or inducing the release of inflammatory mediators that exert their effect upon these receptors.
Costa-Manso
E
E
Department of Allergology and Clinical Immunology, University Clinic, School of Medicine, University of Navarra, Pamplona, Spain.
Sanz
M L
ML
Croce
M
M
Córdoba
H
H
Oehling
A
A
D000328:Adult; D000368:Aged; D001241:Aspirin / Q000009:adverse effects / Q000031:analogs & derivatives / Q000494:pharmacology*; D001249:Asthma / Q000139:chemically induced* / Q000276:immunology; D015536:Down-Regulation / Q000187:drug effects; D004342:Drug Hypersensitivity / Q000276:immunology*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008239:Lysine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000187:drug effects* / Q000378:metabolism
D011943:Receptors, Adrenergic, beta; C047975:cyanopindolol; D010869:Pindolol; D008239:Lysine; D001241:Aspirin; C010395:acetylsalicylic acid lysinate