Glucose transport by the phosphoenolpyruvate:mannose phosphotransferase system in Lactobacillus casei ATCC 393 and its role in carbon catabolite repression.
A 2-deoxy-D-glucose-resistant mutant of a pLZ15-cured derivative of Lactobacillus casei ATCC 393 was isolated on agar medium containing 10 mM 2-deoxy-D-glucose and 5 g lactose I-1. The mutant was impaired in the main glucose transport mechanism, a PTSman-type system. Additionally a proton-motive-force-dependent glucose permease was detected. The growth response and the sugar consumption rates of the wild-type and the PTSman-deficient mutant suggested that the mutated element of the complex IIABCman was, in the wild-type, responsible for a strong repression by glucose and mannose of the lactose and ribose assimilation genes, while assimilation of galactose was only weakly repressed. It is postulated that they are regulated by a different mechanism of catabolite repression.
Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Valencia, Spain.
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Veyrat
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Monedero
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Pérez-Martínez
D001692:Biological Transport / Q000235:genetics; D002455:Cell Division; D003847:Deoxyglucose / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004790:Enzyme Induction; D005690:Galactose / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D012266:Ribose / Q000378:metabolism
D012266:Ribose; D003847:Deoxyglucose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C037992:phosphoenolpyruvate-mannose phosphotransferase; D005947:Glucose; D007785:Lactose; D008358:Mannose; D005690:Galactose