Copy-choice illegitimate DNA recombination revisited.
Nearly precise excision of a transposon related to Tn10 from an Escherichia coli plasmid was used as a model to study illegitimate DNA recombination between short direct repeats. The excision was stimulated 100-1000 times by induction of plasmid single-stranded DNA synthesis and did not involve transfer of DNA from the parental to the progeny molecule. We conclude that it occurred by copy-choice DNA recombination, and propose that other events of recombination between short direct repeats might be a result of the same process.
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
E
E
d'Alençon
M
M
Petranovic
B
B
Michel
P
P
Noirot
A
A
Aucouturier
M
M
Uzest
S D
SD
Ehrlich
D001483:Base Sequence; D004261:DNA Replication / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004274:DNA, Recombinant / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D017911:Inoviridae / Q000502:physiology; D008957:Models, Genetic; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D014764:Viral Proteins