[The correlation between multidrug resistance and glutathione (GSH) in EAC, EAC/ADR and EAC/ADR(R) cell lines].
Determination of GSH was performed by a modified fluorometric method. The experiments showed that the GSH content in resistant cell line (EAC/ADR) was 4 fold higher than that in sensitive cell line (EAC), being 1.25 +/- 0.35 microgram/10(6) cells in EAC/ADR and 0.31 +/- 0.12 microgram/10(6) cells in EAC. When the drug treatment was discontinued for 36 weeks, the sensitivity was partially recovered and the GSH level in EAC/ADR(R) cell parallelly decreased. In addition, vincristine, mitomycin C, actinomycin D and VP-16 showed no effect on the GSH content in the EAC/ADR cell.
Wang
Q D
QD
Henan Institute of Medical Sciences, Zhengzhou.
Li
G D
GD
Liu
M J
MJ
Zhang
Y
Y
Liu
J
J
Zhang
T M
TM
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D008297:Male; D051379:Mice; D016685:Mitomycin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014750:Vincristine / Q000494:pharmacology
D016685:Mitomycin; D014750:Vincristine; D004317:Doxorubicin; D005978:Glutathione