Insertion of the human cytomegalovirus enhancer into a myeloproliferative sarcoma virus long terminal repeat creates a high-expression retroviral vector.
A new retroviral vector is characterized in which the enhancer in the long terminal repeat of myeloproliferative sarcoma virus (MPSV) is combined with the strong and universally active immediate-early human cytomegalovirus (hCMV) enhancer. We demonstrate that insertion of the hCMV enhancer increases the amount of vector-specific mRNA in various rodent cell lines and in human diploid fibroblasts, by at least 3-5-fold. The vector may be particularly useful if high expression of two genes is desired.
Laboratory of Molecular Carcinogenesis, University of Leiden, The Netherlands.
D J
DJ
van den Wollenberg
R C
RC
Hoeben
H
H
van Ormondt
A J
AJ
van der Eb
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008980:Moloney murine sarcoma virus / Q000235:genetics*; D051381:Rats; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*