Selection and study of a Candida molischiana mutant derepressed for beta-glucosidase production.
A mutant strain of Candida molischiana was selected. Analysis of the exocellular activity of Candida molischiana 35M5N grown on different carbon sources revealed that the biosynthesis of beta-glucosidase is derepressed in this yeast strain. The strain is not a hyper-producer mutant. There were no observed differences in the endocellular and parietal activities of the wild and mutant strains. However, the mutant strain produced 35-fold more enzyme than the wild-type in the culture medium with glucose as carbon source. When glucose was used as carbon source, the mutant strain produced 90% more exocellular enzyme than when cellobiose was used as the carbon source.
Chaire de Microbiologie Industrielle et de Génétique des Microorganismes, Ecole Nationale Supérieure Agronomique de Montpellier, France.
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G
Janbon
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A
Arnaud
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P
Galzy
D002175:Candida / Q000201:enzymology* / Q000254:growth & development; D002455:Cell Division; D004794:Enzyme Repression / Q000187:drug effects / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000235:genetics*; D005947:Glucose / Q000494:pharmacology; D009154:Mutation; D012641:Selection, Genetic; D001617:beta-Glucosidase / Q000096:biosynthesis* / Q000235:genetics
D001617:beta-Glucosidase; D005947:Glucose