Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis.
The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the delta lip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.
Laboratoire de Génétique Microbienne, Université Catholique de Louvain, Belgium.
V
V
Dartois
J Y
JY
Coppée
C
C
Colson
A
A
Baulard
D000483:Alleles; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D015870:Gene Expression; D008049:Lipase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids
D008049:Lipase