Immunization and affinity purification of antibodies using resin-immobilized lysine-branched synthetic peptides.
A new method has been developed to raise antibodies against synthetic peptides. A multiple antigenic peptide system (MAP) containing a branched oligolysine was synthesized on a beaded polystyrene polyoxyethylene graft copolymer resin, which acts as a synthetic hapten carrier for use in immunization. The peptides, already attached to the carrier, can be used directly after final deprotection without any further purification steps. The utility of this peptide-carrier conjugate is highlighted by its additional application for affinity purification of antibodies generated.
Max-Planck-Institut für Immunobiologie, Germany.
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Butz
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Rawer
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Rapp
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Birsner
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D015820:Cadherins / Q000276:immunology / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D003598:Cytoskeletal Proteins / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D000922:Immunotoxins / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011092:Polyethylene Glycols; D011107:Polylysine / Q000276:immunology; D011137:Polystyrenes; D011233:Precipitin Tests; D011817:Rabbits; D012116:Resins, Plant; D015534:Trans-Activators; D051176:beta Catenin
D000906:Antibodies; D015820:Cadherins; D003598:Cytoskeletal Proteins; D000922:Immunotoxins; D010455:Peptides; D011137:Polystyrenes; D012116:Resins, Plant; D015534:Trans-Activators; D051176:beta Catenin; D011107:Polylysine; D011092:Polyethylene Glycols