Occurrence of a P450 showing high homology to yeast lanosterol 14-demethylase (P450(14DM)) in the rat liver.
The rat liver cDNA library in lambda gt11 was screened with the antisera against rat liver lanosterol 14 alpha-demethylase (P450(14DM)), and one clone (pRT-9) was isolated. The open reading frame of pRT-9 cDNA showed high homology to yeast P450(14DM) (48.9% in nucleotides and 37.7% in amino acids). Homology of the heme-binding region of pRT-9 protein to that of yeast P450(14DM) was 80%. Three additional regions showing more than 60% homology were identified and one of them was assignable as the distal helix. These findings suggest that pRT-9 protein is P450(14DM) and P450(14DMs) of rat and yeast may be orthologous. This is the first evidence suggesting the occurrence of orthologous P450 in widely diverged species.
Aoyama
Y
Y
Department of Biotechnology, Faculty of Technology, Soka University, Tokyo, Japan.
Funae
Y
Y
Noshiro
M
M
Horiuchi
T
T
Yoshida
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005656:Fungal Proteins / Q000737:chemistry; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D058886:Sterol 14-Demethylase
C548417:Cyp51 protein, rat; D005656:Fungal Proteins; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D058886:Sterol 14-Demethylase