Differential expression of protease inhibitor and small proline-rich protein genes between normal human oral tissue and odontogenic keratocysts.
The technique of differential hybridization was used to compare gene transcription between normal oral mucosa and odontogenic keratocyst lining. Protease inhibitors, elafin and stefin-B as well as beta-actin and two epithelial-specific small proline-rich (spr) proteins, which we have named SPRC and SPRK and which are distinct from salivary proline-rich proteins, were differentially expressed. Increased abundance of alpha I(I) collagen and elafin transcripts was demonstrated in the keratocyst, with decreased abundance of stefin B, SPRC and cytokeratins 4 and 13 transcripts compared to normal palatal mucosa. The deduced protein sequences of SPRC and SPRK were described and compared, and the relative abundance of their respective cDNAs in palatal and keratocyst libraries determined. Identification of factors controlling transcription of these genes could advance our understanding of the development of odontogenic keratocysts.
Division of Dental Surgery, Leeds Dental Institute, West Yorkshire, U.K.
P A
PA
Robinson
J J
JJ
Marley
A S
AS
High
W J
WJ
Hume
D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D055233:Cornified Envelope Proline-Rich Proteins; D055313:Cystatin B; D015891:Cystatins / Q000235:genetics*; D015342:DNA Probes; D005786:Gene Expression Regulation; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009807:Odontogenic Cysts / Q000235:genetics*; D011392:Proline / Q000235:genetics*; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000235:genetics*; D015842:Serine Proteinase Inhibitors / Q000235:genetics*; D014158:Transcription, Genetic
D000199:Actins; C524994:CSTB protein, human; D055233:Cornified Envelope Proline-Rich Proteins; D015891:Cystatins; D015342:DNA Probes; D008565:Membrane Proteins; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors; D055313:Cystatin B; D011392:Proline