Neutralization enzyme immunoassay for influenza virus.
A neutralization enzyme immunoassay (N-EIA) was developed for the detection of antibody titer rises in sera of patients infected with influenza A (H3N2) virus. In this N-EIA, a selected strain of influenza A (H3N2) virus was added to monolayers of LLC-MK2 cells in microtiter plates. After 24 h, the replicated virus could be demonstrated with a virus-specific enzyme-labeled monoclonal antibody. Preincubation of the influenza virus with convalescent-phase sera of patients infected with influenza A (H3N2) virus resulted 1 day later in decreased absorbance values that could be used for calculation of neutralization titers. From use of paired serum samples from 10 patients with a history of flu-like symptoms, the results obtained with N-EIA correlated well (r = 0.83) with those of the standard hemagglutination inhibition test.
Eijkman-Winkler Laboratory of Medical Microbiology, University Hospital Utrecht, The Netherlands.
C A
CA
Benne
M
M
Harmsen
J C
JC
De Jong
C A
CA
Kraaijeveld
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral; D002460:Cell Line; D005069:Evaluation Studies as Topic; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology* / Q000502:physiology; D007251:Influenza, Human / Q000175:diagnosis* / Q000276:immunology; D051379:Mice; D009500:Neutralization Tests / Q000379:methods*; D012698:Serologic Tests / Q000379:methods; D014776:Virus Cultivation; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral