[Immunohistochemical studies of mononuclear cellular infiltrates of papillary, follicular and anaplastic thyroid cancers].
The mononuclear cellular infiltrate of thyroid neoplasms was examined by immunohistochemical techniques and compared to normal thyroid (control 1) and untreated Graves' disease tissue (control 2). Using a panel of monoclonal antibodies T-, B cells and macrophage antigens as well as the expression of activation-associated antigens and of MHC class II molecules were examined. In comparison to control 1 an increase of positive cells could be detected in carcinomas. This increase was significant for CD3+ and CD8+ T cells, for CD4+ macrophages and for HLA-DP+ cells in all types of carcinomas. In addition, in anaplastic carcinomas a significant increase of B cells, RM3/1+ macrophages, IL-2R+, ICAM-1+ and HLA-DQ+ cells was observed as well as of HLA-DR+ cells in follicular and of CD4+ T cells in papillary carcinomas. A comparison with control 2 revealed a significant increase of CD4+ cells (T cells and macrophages) in papillary and of CD8+ and RM3/1+ cells in anaplastic carcinomas. Our findings, an obvious increase of cell populations and of an upregulated expression of activation-associated antigens on these cells, indicated an immunological reaction caused by neoplastic thyrocytes. Further functional studies are however necessary.
Kornfehl
J
J
Univ. Klinik HNO, Universität Wien.
Wilfing
A
A
Hermann
M
M
D018263:Adenocarcinoma, Follicular / Q000276:immunology* / Q000473:pathology; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D002277:Carcinoma / Q000276:immunology* / Q000473:pathology; D002291:Carcinoma, Papillary / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000032:analysis; D005260:Female; D006111:Graves Disease / Q000276:immunology / Q000473:pathology; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000473:pathology; D013961:Thyroid Gland / Q000276:immunology / Q000473:pathology; D013964:Thyroid Neoplasms / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016207:Cytokines; D006680:HLA Antigens
Immunohistochemische Untersuchungen des mononukleären zellulären Infiltrates von papillären, follikulären und anaplastischen Schilddrüsenkarzinomen.