A sensitive reporter cell line for HIV-1 tat activity, HIV-1 inhibitors, and T cell activation effects.
The production and characterization of a cell line for quantitative HIV-1 Tat function and T cell activation assays is described. 1G5 is a clonal cell line derived from Jurkat T cells stably transfected with a luciferase gene driven by an HIV-1 long terminal repeat (HIV-LTR). The 1G5 clone was selected for low basal luciferase activity, susceptibility to HIV infection, and high responsiveness to Tat and T cell activation signals. A 10 to 1000-fold increase in luciferase activity after transfection or infection with tat-expressing vectors or HIV was observed. Equivalent levels of expression were detected after stimulation with T cell mitogens. The characteristics of 1G5 make it a valuable reagent for studies of HIV infection, HIV regulatory agents, and other T cell or HIV-activating factors, and for screening potential anti-HIV therapeutic agents.
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
E
E
Aguilar-Cordova
J
J
Chinen
L
L
Donehower
D E
DE
Lewis
J W
JW
Belmont
D000595:Amino Acid Sequence; D002460:Cell Line; D017931:DNA Primers; D015696:Gene Products, tat / Q000378:metabolism*; D015756:Genes, tat; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D015215:Zidovudine / Q000494:pharmacology; D054322:tat Gene Products, Human Immunodeficiency Virus
D017931:DNA Primers; D015696:Gene Products, tat; D054322:tat Gene Products, Human Immunodeficiency Virus; D015215:Zidovudine; D008156:Luciferases