Hemin binding to DNA with bis-dentate acridine intercalator.
Hemin was covalently connected to two 9-aminoacridines (9AA) through its two propionates, and the binding properties of this bis-dentate compound (hemin(9AA)2) to DNA were examined by visible absorption spectroscopy. The binding affinity of the hemin(9AA)2 was found to be higher than that of the hemin, and this should be attributed to the two linked acridine moieties. The binding constant (K) and the number of binding sites per nucleotide (n) were estimated by Scatchard plot analyses. Though the order of the K value of hemin(9AA)2 was similar to that of 9AA, the hemin(9AA)2 was analyzed to have a smaller n value, the order of which was of about 10(-4). The small n value may reflect the sequence specificity of the bis-dentate hemin(9AA)2 on binding to the DNA.
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
T
T
Uno
N
N
Sowa
S
S
Shimabayashi
D000585:Aminacrine / Q000378:metabolism*; D000818:Animals; D004247:DNA / Q000378:metabolism*; D006427:Hemin / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D012474:Salmon
D006427:Hemin; D000585:Aminacrine; D004247:DNA