The mechanism of chlorpromazine-induced red blood cell swelling.
1. Chlorpromazine (CPZ)-induced red blood cell (RBC) swelling was investigated by determination of electrolyte, mean cell volume (MCV) and water content changes in CPZ-treated cells. 2. CPZ-induced RBC swelling is dose-, hematocrit- and pH-dependent, and is associated with a net increase in RBC monovalent cation and Cl- contents. 3. The partition coefficient (Kp) for the CPZ-RBC membrane interaction is pH dependent (Kp = 2500 at pH 7.8; Kp = 840 at pH 6.8). 4. Despite the pH dependence of Kp values an equal number of CPZ molecules is required to induce a 12% increase in MCV at pH 7.8 and 6.8.
Children's Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine 19104.
A S
AS
Cornelius
M P
MP
Reilly
M
M
Suzuki
T
T
Asakura
K
K
Horiuchi
D000328:Adult; D048429:Cell Size / Q000187:drug effects; D002712:Chlorides / Q000097:blood; D002746:Chlorpromazine / Q000494:pharmacology*; D004911:Erythrocyte Volume / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000378:metabolism; D007700:Kinetics; D011188:Potassium / Q000097:blood; D012964:Sodium / Q000097:blood
D002712:Chlorides; D012964:Sodium; D011188:Potassium; D002746:Chlorpromazine