Poly(ADP-ribose) molecules formed during DNA repair in vivo.
We have found that human cells respond to treatment with an alkylating carcinogen by producing a specific, highly conserved array of poly(ADP-ribose) molecules. Using in situ radiolabeling and boronate affinity chromatography, we have been able to isolate ADP-ribose polymers from living cells and to quantify individual molecular size classes on high resolution polyacrylamide gels. Despite carcinogen-induced changes in poly(ADP-ribose) turnover, the relative frequency of linear polymers was strictly conserved. However, the abundance of branched polymers with high affinity for histones increased 2.6-fold. These polymers were degraded more slowly than linear polymers. Our results indicate that the poly(ADP-ribose) molecules involved in the shuttling of histones on DNA in vitro also play a role in the repair of DNA damage in vivo.
Malanga
M
M
Institute of Pharmacology and Toxicology, University of Zürich-Tierspital, Switzerland.
Althaus
F R
FR
D000478:Alkylation; D002460:Cell Line; D002846:Chromatography, Affinity; D004249:DNA Damage; D004260:DNA Repair; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis* / Q000302:isolation & purification
D011064:Poly Adenosine Diphosphate Ribose