Entire nucleotide sequence and characterization of a hepatitis C virus of genotype V/3a.
The entire nucleotide sequence of a hepatitis C virus (HCV) genome (NZL1) of genotype V/3a was determined from overlapping cDNA clones obtained from a human carrier in New Zealand. It comprised 9425 nucleotides (nt) including a 5'-untranslated region of 339 nt, a single large open reading frame encoding a polyprotein of 3021 amino acids, a 3'-untranslated region of 23 nt, and 3'-terminal poly(U) stretches of variable lengths. The NZL1 genome was compared with 15 HCV isolates of other genotypes for which the full-length sequence has been determined. It differed from them by 31.1 to 34.3% in nucleotide sequence identity and by 24.5 to 29.1% in amino acid sequence identity, confirming the distinction of genotype V/3a from the other isolates.
First Department of Internal Medicine, Yamanashi Medical College, Japan.
M
M
Sakamoto
Y
Y
Akahane
F
F
Tsuda
T
T
Tanaka
D G
DG
Woodfield
H
H
Okamoto
D000595:Amino Acid Sequence; D001483:Base Sequence; D002353:Carrier State; D018076:DNA, Complementary; D005814:Genes, Viral / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D016679:Genome, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D006526:Hepatitis C / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009520:New Zealand; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary