[Interaction of rabbit liver annexins with various divalent metal ions: competition of proteins with each other and change in the order of annexin binding with phospholipid vesicles].
The interaction of a natural annexin mixture isolated from rabbit liver with liposomes prepared from soybean azolectin has been studied. With a limited number of accessible binding sites when liposomes can bind no more than 45% of the annexins added to the medium the number pattern of total annexin binding is very different in the presence of various ions, especially in the presence of Ca2+ and Sr2+ when distinct maxima and minima of binding are seen. The observed changes are due to the competition of different annexins for the binding sites on the liposomes. The order of individual annexin binding was shown to alter significantly depending on the nature of the ion (Ca2+, Ba2+, Sr2+, Mn2+, Ni2+) present in the medium. The annexins are proposed to be just one constituent of a complex intracellular system interacting with ions of various bivalent metals.
Mel'gunov
V I
VI
Nabokina
S M
SM
D000818:Animals; D017302:Annexins / Q000378:metabolism*; D001667:Binding, Competitive; D008081:Liposomes; D008099:Liver / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011817:Rabbits
D017302:Annexins; D008081:Liposomes; D008670:Metals; D010743:Phospholipids
Vzaimodeĭstvie anneksinov pecheni krolika s ionami razlichnykh dvukhvalentnykh metallov: konkurentsiia belkov drug s drugom i izmenenie poriadka sviazyvaniia anneksinov s fosfolipidnymi vezikulami.