Crosslinking and cleavage of pBR322 DNA photosensitized by 7-methylpyrido[3,4-c]psoralen.
7-Methylpyrido[3,4-c]psoralen (7-MPP) had been designed to be a monofunctional sensitizer in which the pyrone double bond, being engaged in a pyridine ring, would be incapable of participating in the formation of a cyclobutane ring with a DNA component. However, one example of photosensitization of thymine-thymine dimer formation in DNA by 7-MPP had been reported. This paper proves that 7-MPP can sensitize interstrand crosslinks in pBR322 DNA. It also proves that 7-MPP photosensitizes double strand cleavage reactions in the DNA with an unusually large degree of site selectivity.
Chen
X
X
Department of Chemistry, University of Illinois, Chicago 60607-7061.
Kagan
J
J
D002273:Carcinogens / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000187:drug effects / Q000528:radiation effects; D004926:Escherichia coli; D011564:Furocoumarins / Q000494:pharmacology*; D015394:Molecular Structure; D017319:Photosensitizing Agents / Q000494:pharmacology; D010957:Plasmids / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays
D002273:Carcinogens; D004269:DNA, Bacterial; D011564:Furocoumarins; D017319:Photosensitizing Agents; C081211:7-methylpyrido(4,3-c)psoralen