The presence of two types of gene that encode the chlorophyll a/b-binding protein (LHCPII) and their light-independent expression in pine (Pinus thunbergii).
A cDNA clone (pPDLHC3117) for LHCPII of pine (Pinus thunbergii) was isolated and sequenced. From a comparison of the deduced amino acid sequence with those of proteins from other seed plants, pPDLHC3117 was identified as encoding a type I LHCPII. Specific probes for genes for type I and type II LHCPII were made from pPDLHC3117 and a type II cDNA (pPDLHC2176), respectively. Northern blotting with the specific probes showed that both types of LHCPII were expressed in dark-grown seedlings of pine.
Forestry and Forest Products Research Institute, Ibaraki, Japan.
N
N
Yamamoto
K
K
Kojima
M
M
Matsuoka
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D005786:Gene Expression Regulation; D017343:Genes, Plant; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000096:biosynthesis / Q000235:genetics*; D010802:Phylogeny; D010944:Plants / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014197:Trees / Q000235:genetics* / Q000378:metabolism*
D018076:DNA, Complementary; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins