Sequence and activity of the rat PB-inducible aldehyde dehydrogenase promoter.
The 5' flanking region of the rat PB inducible aldehyde dehydrogenase gene was isolated and the sequence from +42 to -1339 was determined. This sequence contains several putative binding sites for the liver-enriched factors HNF3 and DBP as well as a GRE and several possible AP1 sites. A TATA and CCAAT motif were assigned at positions -26 and -53. A promoter construct containing the -1339 bp of the aldehyde dehydrogenase 5' flanking region was active when transfected into both H411 and HepG2 liver cell lines.
Department of Cell Biology, New York University School of Medicine, New York, NY 10016.
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Shaw
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Adesnik
D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010634:Phenobarbital / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D004247:DNA; D000444:Aldehyde Dehydrogenase; D010634:Phenobarbital