[Ectopic bone induction by human fetal enamel proteins].
Mixture of amelogenin, enamelin and soluble dentin proteins with plaster of Paris and distilled water were implanted in the left thigh-muscle pouch of C57BL/6 T mice, and PLP or BSA/PLP were implanted in the contralateral limbs for controls. The hind limbs were examined by means of microradiographic and histological methods three weeks after the implantation. Implants of PLP, BSA/PLP or E/PLP did not evoke formation of new cartilage or bone. Roentgenography showed highly mineralized tissues in the implantation areas of A/PLP or D/PLP, histological examination confirmed this as induced new bone or cartilage formation. Thus it indicates that amelogenins and soluble dentin proteins have bone induction activity as bone morphogenetic protein, they could induce the differentiation of mesenchymal cell in the muscles into chondrocyte and osteocyte.
Department of Stomatology, School of Medical, Ji-nan University.
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Wang
D053523:Amelogenin; D000818:Animals; D019485:Bone Morphogenetic Proteins; D002133:Calcium Sulfate / Q000494:pharmacology; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D005333:Fetus; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010012:Osteogenesis / Q000187:drug effects*; D010750:Phosphoproteins / Q000494:pharmacology*; D019736:Prostheses and Implants; D011506:Proteins / Q000494:pharmacology
D053523:Amelogenin; C507200:Amelx protein, mouse; D019485:Bone Morphogenetic Proteins; D003746:Dental Enamel Proteins; D006133:Growth Substances; D010750:Phosphoproteins; D011506:Proteins; C017630:phosphophoryn; C038456:tuftelin; D002133:Calcium Sulfate