Technical improvements in corrosion casting of small specimens: a study on mesonephric tubules and vessels of chicken embryos.
The injection technique for corrosion casting of small, embryonic material can be improved by using a "chemical ligature" (cyanoacrylate). With this simple method, leakage of the resin at the injection site is prevented and the mechanical stability of the cannula-vessel coupling is improved. The blood vascular system of chicken embryos as small as Hamburger-Hamilton stage 24 (approximately 4.5 days of incubation) has successfully been injected using this procedure. Corrosion casts of the mesonephric tubular system have been made in a similar manner. Additionally, a simple way for secure transport of the fragile casts by immersion in 10% gelatin is suggested.
Dept. Anatomy and Embryology, Veterinary Faculty, Autonomous University of Barcelona, Spain.
A
A
Carretero
H
H
Ditrich
M
M
Navarro
H
H
Splechtna
J
J
Ruberte
D000818:Animals; D001808:Blood Vessels / Q000648:ultrastructure*; D002642:Chick Embryo; D003487:Cyanoacrylates; D007684:Kidney Tubules / Q000648:ultrastructure; D008650:Mesonephros / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D012116:Resins, Plant
D003487:Cyanoacrylates; D012116:Resins, Plant