Two GTPs are hydrolysed on two molecules of EF-Tu for each elongation cycle during code translation.
A new experimental design has been used to determine the number of GTPs hydrolysed per peptide bond in EF-Tu function in a poly(U)-translation system. We find that two GTPs are consumed for every amino acid incorporated into the nascent poly(Phe)-chains, in accordance with previous findings with other techniques. These results necessitate a revision of current views concerning E coli translation; also new schemes for ribosome function are discussed.
Department of Molecular Biology, University of Uppsala, Sweden.
J
J
Scoble
N
N
Bilgin
M
M
Ehrenberg
D002021:Buffers; D002855:Chromatography, Thin Layer; D004734:Energy Metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006868:Hydrolysis; D010441:Peptide Chain Elongation, Translational; D010444:Peptide Elongation Factor Tu / Q000378:metabolism*; D011072:Poly U / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics
D002021:Buffers; D011072:Poly U; D006160:Guanosine Triphosphate; D010444:Peptide Elongation Factor Tu