Structure and orientation of apo B-100 peptides into a lipid bilayer.
Peptides corresponding to lipid binding domains of Apo B-100 were synthesized, purified, and incubated with dimyristoylphosphatidylcholine (DMPC) liposomes. The secondary structure of the apo B-100 peptide-lipid complexes was evaluated by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Those peptides belonging to the hydrophobic "core" domain of apo B-100 when associated with phospholipids were rich in beta sheet structure; a predominant alpha helical conformation was shown to be associated with one peptide located in a surface region of apo B-100. IR dichroic spectra revealed, in the case of the "core" peptides, that the beta sheet component is the only oriented structure with respect to the phospholipid acyl chains. This orientation of the beta sheet was recently found in LDL particles after proteolytic digestion by trypsin (Goormaghtigh, E., Cabiaux, V., De Meutter, J., Rosseneu, M., and Ruysschaert, J. M., 1993, Biochemistry 32, 6104-6110). Altogether, the data suggest that beta sheet, present in a high proportion in the native apo B-100, is probably another protein structure in addition to the amphipathic helix which strongly interacts with the lipid outer layer surrounding the LDL particle.
Laboratoire de Chimie Physique des Macromolécules aux Interfaces, Free University of Brussels, Belgium.
L
L
Lins
R
R
Brasseur
M
M
Rosseneu
C Y
CY
Yang
D A
DA
Sparrow
J T
JT
Sparrow
A M
AM
Gotto
J M
JM
Ruysschaert
D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000737:chemistry*; D001665:Binding Sites; D004134:Dimyristoylphosphatidylcholine; D007202:Indicators and Reagents; D008051:Lipid Bilayers; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D007202:Indicators and Reagents; D008051:Lipid Bilayers; D010446:Peptide Fragments; D004134:Dimyristoylphosphatidylcholine