Effect of desialylation on low density lipoproteins: comparative study before and after oxidative stress.
Aim of our study was to investigate the effect of the desialylation induced by neuraminidase treatment on low density lipoprotein susceptibility to peroxidative stress induced by incubation with copper ions. Our results show that peroxidative stress induces the formation of aggregates that was not observed in desialylated low density lipoproteins. An increase in thiobarbituric reactive substances and a decrease in polyunsaturated fatty acids content have been shown in oxidized LDL. These modifications were less pronounced in oxidized low density lipoproteins previously treated by neuraminidase. The present data suggest a lower susceptibility to peroxidative stress in previously desialylated low density lipoproteins.
Department of Biochemistry, Rangueil University Hospital, Toulouse, France.
N
N
Dousset
J C
JC
Dousset
M
M
Taus
G
G
Ferretti
G
G
Curatola
M L
ML
Soléra
P
P
Valdiguié
D001055:Apolipoproteins B / Q000032:analysis / Q000302:isolation & purification; D003300:Copper; D004591:Electrophoresis, Polyacrylamide Gel; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D009439:Neuraminidase; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D001055:Apolipoproteins B; D005231:Fatty Acids, Unsaturated; D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper; D009439:Neuraminidase