A physical map of two clusters containing the genes for six proinflammatory receptors.
The genes encoding for six receptors involved in the proinflammatory response lie on different chromosomes. Two receptors for N-formylpeptides (FPR1, FPR2), one homologue of these (FPRL2), and the receptor for complement fragment C5a (C5aR) are encoded by four genes mapped to human chromosome 19. The genes encoding two receptors for Interleukin-8 (IL8RA, IL8RB) have been located on human chromosome 2. In this report we describe the physical linkage between these genes in two different clusters. DNA fragments obtained by digestion with several restriction enzymes were separated by pulsed field gel electrophoresis. Nylon filters were hybridized with probes corresponding to the complete translated sequences of these genes. These probes were obtained from a human neutrophil cDNA-library. The four genes on chromosome 19 are contained in a 200 kilobase (kb) fragment. Both Interleukin-8 receptors are on a 150 kb fragment. The complete translated sequences for these genes were amplified from genomic DNA, indicating that they are contained in a single exon.
Servicio de Immunología, Hospital Central de Asturias, Oviedo, Spain.
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V
Alvarez
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E
Coto
F
F
Setién
C
C
López-Larrea
D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D002888:Chromosomes, Human, Pair 19; D002889:Chromosomes, Human, Pair 2; D005561:Formates; D008040:Genetic Linkage; D015894:Genome, Human; D006801:Humans; D007249:Inflammation / Q000235:genetics; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000235:genetics*; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000235:genetics*; D018123:Receptors, Interleukin / Q000235:genetics*; D023062:Receptors, Interleukin-8A; D018000:Receptors, Peptide / Q000235:genetics*
D005561:Formates; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D018000:Receptors, Peptide; C030544:formic acid