An analysis of two spectral properties of vertebrate visual pigments.
Spectral data are scrutinized on two properties: (1) the chromophore-induced wavelength shift, i.e. the variation in absorbance maximum (lambda max) upon exchanging the vitamin A1-based chromophore with one based on vitamin A2 in the same opsin; (2) the half-band width (HBW) variation as a function of the reciprocal of the peak absorbance (lambda max)-1. It is shown that in an extended spectral range that includes the UV and when data are plotted in wavenumbers, the chromophore-induced shifts can be approximated with a parabola, whose minimum occurs near 23,000 cm-1 (approximately 430 nm). Similarly, HBW variations can also be fitted with parabolas, however, these show maxima near 430 nm. The theoretical implications of the two phenomena concerning lambda max tuning in vertebrate visual pigments are discussed.
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Laboratory of Sensory Physiology, Marine Biological Laboratory, Woods Hole, MA 02543.
D000663:Amphibians; D000818:Animals; D005399:Fishes; D015591:Microspectrophotometry; D012168:Retinal Pigments / Q000032:analysis*; D013045:Species Specificity; D013057:Spectrum Analysis; D014466:Ultraviolet Rays; D014801:Vitamin A / Q000032:analysis
D012168:Retinal Pigments; D014801:Vitamin A