Calcium binding properties of rabbit liver protein disulfide isomerase.
We have characterized the Ca2+ binding properties of protein disulfide isomerase (PDI). It binds 19 mol Ca2+/mol protein with low affinity, which is reduced by increasing the ionic strength. Ca2+ induced conformational changes detected by UV difference spectroscopy. These Ca2+ binding properties resemble those of a sea urchin egg 58 kDa protein.
Lebeche
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D
Department of Physiology, Boston University School of Medicine, MA 02118.
Lucero
H A
HA
Kaminer
B
B
D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007535:Isomerases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D019704:Protein Disulfide-Isomerases; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
D002135:Calcium-Binding Proteins; D007535:Isomerases; D019704:Protein Disulfide-Isomerases; D002118:Calcium