Stability of oxytetracycline hydrochloride in eye-drops, prepared in pharmacies.
In the ophthalmological practice the so-called "Oxytetracycline-eye-drop" is frequently used, prepared in pharmacies ex tempore with a short self-life (3 days). In this paper a TLC-densitometric method is described for the determination of the degradation products of oxytetracycline HCl (OTC) formed in the "Oxytetracycline-eye-drop". A chloroform-methanol-water (65 + 25 + 5 v/v) solvent system is applied on silica gel TLC plate, predeveloped with saturated disodium ethylenediamine-tetraacetate aqueous solution. UV densitometry at 254 nm is used for quantitative determination. The chemical stability of OTC in different eye-drops as well as the effects of the pH and the mode of the preparation and storage conditions on the decomposition process are discussed.
National Institute of Pharmacy, Budapest.
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D002855:Chromatography, Thin Layer; D003720:Densitometry; D004355:Drug Stability; D009883:Ophthalmic Solutions; D010118:Oxytetracycline / Q000737:chemistry*; D012997:Solvents; D013053:Spectrophotometry; D013997:Time Factors
D009883:Ophthalmic Solutions; D012997:Solvents; D010118:Oxytetracycline