A phylogenetic analysis of Schistosoma haematobium group species based on randomly amplified polymorphic DNA.
Randomly amplified polymorphic DNA (RAPD) profiles were produced using four oligonucleotide primers with genomic DNA from 15 isolates of schistosome. Both inter- and intraspecific variation were noted. Intraspecific variation was greater for two species of the S. haematobium group (S. haematobium and S. intercalatum) than for S. mansoni. The inferred phylogeny placed S. curassoni and S. bovis as sister groups to S. mansoni-S. rodhaini group. S. mattheei and S. leiperi formed a separate lineage. The results confirm that RAPD profiles may be used for both strain and species differentiation and for the generation of phylogenetic trees.
Department of Zoology, Natural History Museum, London, U.K.
A
A
Kaukas
E
E
Dias Neto
A J
AJ
Simpson
V R
VR
Southgate
D
D
Rollinson
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012548:Schistosoma haematobium / Q000145:classification / Q000235:genetics*
D017931:DNA Primers; D004247:DNA