Influence of epidermal growth factor and transforming growth factor-alpha on in vitro maturation of cumulus cell-enclosed bovine oocytes in a defined medium.
This study was carried out to determine the effects of growth factors (epidermal growth factor, transforming growth factors-alpha and -beta 1, basic fibroblast growth factor, insulin), gonadotrophins (LH, FSH), and fetal bovine serum added to TCM199 medium on cumulus expansion and fertilization during in vitro maturation, and on subsequent embryonic development of bovine cumulus cell-enclosed oocytes. Epidermal growth factor, transforming growth factor-alpha, LH and FSH enhanced cumulus expansion and oocyte fertilizability. No significant effect was achieved with transforming growth factor-beta 1 nor with basic fibroblast growth factor. No additive stimulation on cumulus expansion and oocyte fertilizability was observed when epidermal growth factor was combined with LH or FSH. The addition of either epidermal growth factor or transforming growth factor-alpha to the maturation medium increased the number of fertilized ova that developed to the blastocyst stage. These results demonstrate the potential use of epidermal growth factor and transforming growth factor-alpha in obtaining high quality mature bovine oocytes for in vitro fertilization.
Research Institute for the Functional Peptides, Yamagata, Japan.
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K
Kobayashi
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S
Yamashita
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H
Hoshi
D000818:Animals; D002417:Cattle; D003470:Culture Media; D005314:Embryonic and Fetal Development / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects; D009866:Oogenesis / Q000187:drug effects*; D016211:Transforming Growth Factor alpha / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D003470:Culture Media; D006133:Growth Substances; D007328:Insulin; D016211:Transforming Growth Factor alpha; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone