Selective inhibition of dexamethasone-induced apoptosis in rat thymocytes by herbimycin A.
DNA fragmentation and cell death in rat thymocytes induced by dexamethasone were inhibited by herbimycin A but not by the other inhibitors of tyrosine kinase including genistein and tyrphostin. Herbimycin A also prevented the inter-nucleosomal DNA fragmentation induced by dexamethasone. On the contrary, apoptosis induced by DNA topoisomerase inhibitors such as camptothecin and etoposide were not affected by herbimycin A. These results demonstrate that dexamethasone-induced apoptosis is specifically inhibited by herbimycin A.
Lee
E
E
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
Miura
M
M
Yoshinari
M
M
Iwai
H
H
Kariya
K
K
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D016227:Benzoquinones; D002166:Camptothecin / Q000494:pharmacology; D002396:Catechols / Q000494:pharmacology; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D004249:DNA Damage; D003907:Dexamethasone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005047:Etoposide / Q000494:pharmacology; D019833:Genistein; D007529:Isoflavones / Q000494:pharmacology; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D047029:Lactams, Macrocyclic; D009570:Nitriles / Q000494:pharmacology; D009707:Nucleosomes / Q000187:drug effects / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011809:Quinones / Q000494:pharmacology*; D051381:Rats; D017828:Rifabutin / Q000031:analogs & derivatives; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D059004:Topoisomerase I Inhibitors; D020032:Tyrphostins
D016227:Benzoquinones; D002396:Catechols; D007529:Isoflavones; D047029:Lactams, Macrocyclic; D009570:Nitriles; D009707:Nucleosomes; D011809:Quinones; D059004:Topoisomerase I Inhibitors; D020032:Tyrphostins; C060152:tyrphostin 47; D017828:Rifabutin; D005047:Etoposide; C020754:herbimycin; D003907:Dexamethasone; D004247:DNA; D019833:Genistein; D007770:L-Lactate Dehydrogenase; D011505:Protein-Tyrosine Kinases; D002166:Camptothecin