A stable dicentric chromosome: both centromeres develop kinetochores and attach to the spindle in monocentric and dicentric configuration.
A stable, dicentric human chromosome, which is known from light microscopy to show a 50:50 distribution between monocentric/dicentric appearance, was examined by conventional electron microscopy and after labelling the centromere with anticentromere antibodies from CREST serum. Both centromeres of the chromosome developed kinetochores whether in monocentric or dicentric configuration. The eight monocentrics observed had all developed kinetochores at the centromere outside the constriction; at least six of them also had kinetochores at the centromere in the constriction. The dicentrics from glutaraldehyde fixed cells had spindle microtubules attached to both kinetochore sets irrespective of monocentric/dicentric configuration. The chromosome thus appeared to use both centromeres, either equally or with one serving a chromatid adhesion function while the second was used for transport along the spindle.
Institute of Medical Biochemistry and Genetics, Panum Institute, Copenhagen N, Denmark.
A
A
Wandall
D002460:Cell Line; D002503:Centromere / Q000378:metabolism* / Q000648:ultrastructure; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D008941:Spindle Apparatus / Q000378:metabolism* / Q000648:ultrastructure