Changes in nuclear macromolecules and nucleolar volume associated with selective ribosomal cistron activation by ethylene.
Pieces of leaf base tissue from the equatorial region of the third turgid onion (Allium cepa L.; yellow, sweet Spanish, quiescent tissue) leaf base with two visible major nucleoli (volume = 0.51 microns3) and two inactive minor nucleolar organizer regions (NORs) in the outer epidermal cells were placed in shallow water (time = T0 control). After 3 h (T3 control), the two visible nucleoli enlarged (178% of T0) and changed shape (from 88% round and 12% oval at T0 to 5% round, 68% oval, 20% elongated-oval, and 7% dumbbell). The minor NORs remained inactive. Nuclear RNA and non-histone protein (nHP) increased (RNA = 128% and nHP = 134%, compared with T0 data, set at 100%). The content of DNA and histone protein (H) in nuclei remained unchanged. Major nucleoli in pieces of onion placed in 10(-5) M ethylene enlarged and minor nucleoli became visible in 33% of the nuclei; i.e., ethylene acted as a selective ribosomal cistron regulator. The nucleolar volume (212% of T0) was statistically greater than those for T0 and T3. DNA and H remained unchanged (not statistically different) but RNA (159% of T0) and nHP (169% of T0) were greater (statistically significant) than those for T0 and T3 controls. Cobalt chloride (10(-5) M Co) alone or in 10(-5) M ethylene (Co + E) inhibited the enlargement of some of the major nucleoli and completely inhibited the appearance of minor nucleoli. The nucleolar volumes for the cobalt and for cobalt-ethylene treatments were significantly less than those for T3 controls and ethylene treatment at T3. DNA and histone did not change.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Biology, Indiana University Northwest, Gary 46408.
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Bhattacharya
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Karagiannis
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Pappelis
D000490:Allium / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000187:drug effects* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000187:drug effects; D004247:DNA / Q000187:drug effects; D005030:Ethylenes / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D017343:Genes, Plant / Q000187:drug effects*; D006657:Histones / Q000187:drug effects; D009687:Nuclear Proteins / Q000187:drug effects; D015138:RNA, Nuclear / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects*
D002868:Chromosomal Proteins, Non-Histone; D005030:Ethylenes; D006657:Histones; D009687:Nuclear Proteins; D015138:RNA, Nuclear; D004247:DNA; C036216:ethylene