A novel peptide in the AKH/RPCH family isolated from the corpora cardiaca of the Emperor dragonfly, Anax imperator.
Using a heterologous (in locusts and cockroaches) and a homologous bioassay, we have isolated the neuropeptide pGlu-Val-Asn-Phe-Ser-Pro-Ser-Trp-NH2 from extracts of corpora cardiaca of the Emperor dragonfly, Anax imperator. The sequence elucidation was achieved by Edman degradation of the deblocked peptide and by electrospray mass spectrometry. Low concentrations of the synthetic peptide injected into the Emperor dragonfly increased the hemolymph lipid concentration, suggesting a possible role of the peptide in lipid homeostasis during flight. Therefore, it is named Ani-AKH, Anax imperator adipokinetic hormone.
Zoology Department, University of Cape Town, Rondebosch, South Africa.
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Janssens
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Kellner
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D007301:Insect Hormones / Q000302:isolation & purification*; D007313:Insecta; D007447:Invertebrate Hormones / Q000302:isolation & purification*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000302:isolation & purification*; D009842:Oligopeptides / Q000302:isolation & purification*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives
D007301:Insect Hormones; D007447:Invertebrate Hormones; D009479:Neuropeptides; D009842:Oligopeptides; C044833:adipokinetic hormone; C022059:red pigment-concentrating hormone; D011761:Pyrrolidonecarboxylic Acid