The induction of murine tumor infiltrating lymphocytes (TIL) by interleukin-2 or T cell growth factor.
Mice were injected in the foot pad with either 5 x 10(5) syngeneic plasmacytoma (MOPC104E) or fibrosarcoma cells (Meth A). Lymph nodes containing tumor cells were harvested 14 days later and cultured. In the presence of recombinant interleukin-2 (r-IL-2) predominantly tumor cells proliferated. Culture with T cell growth factor (TCGF) resulted in the growth of lymphoid cells. Concanavalin A (Con A) had only a modest effect on elimination of tumor cells in the culture. Tumor-infiltrating lymphocytes (TIL) prepared from the lymph nodes showed specific tumor-neutralizing activity when grown in the presence of TCGF. In vitro examination revealed that Meth A cells could not be lysed by TIL, while TIL from MOPC tumors showed tumor specific activity. This study may explain negative results in human trials with TIL induced by IL-2 alone.
First Department of Surgery, Kyoto University, Faculty of Medicine, Japan.
N
N
Kan
K
K
Mise
M
M
Nakanishi
T
T
Okino
T
T
Harada
Y
Y
Ichinose
Y
Y
Moriguchi
T
T
Sugie
L
L
Li
M
M
Imamura
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005354:Fibrosarcoma / Q000473:pathology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D008207:Lymphatic Metastasis; D016246:Lymphocytes, Tumor-Infiltrating / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D010954:Plasmacytoma / Q000473:pathology; D051381:Rats; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D003208:Concanavalin A