C-type natriuretic peptide in the human central nervous system: distribution and molecular form.
The distribution and molecular form of C-type natriuretic peptide (CNP) in the human central nervous system were studied with a specific radioimmunoassay for CNP-22. Immunoreactive (IR-) CNP was detectable in all regions of the brain examined (cerebral cortex, thalamus, hypothalamus, pons, and cerebellum) (0.21-0.81 pmol/g wet tissue, n = 4). The highest concentration of IR-CNP was found in the spinal cord at 1.83 +/- 0.13 pmol/g wet tissue (mean +/- SD, n = 3). Reversed-phase high performance liquid chromatography revealed a major peak migrating at the position corresponding to synthetic human CNP-53 and minor peaks comigrating with synthetic CNP-22 and the methionine-oxidized form of CNP-22, respectively. These findings suggest that IR-CNP is widely present in the human central nervous system mainly in a high molecular weight form as the major component and in the molecular form of CNP-22 as the minor component.
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
K
K
Totsune
K
K
Takahashi
M
M
Ohneda
K
K
Itoi
O
O
Murakami
T
T
Mouri
D000368:Aged; D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000737:chemistry; D002490:Central Nervous System / Q000737:chemistry*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000737:chemistry; D011863:Radioimmunoassay
D009419:Nerve Tissue Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor