Regulation of growth hormone receptor and binding protein expression in domestic species.
Growth hormone receptor (GHR) expression has been analyzed at the RNA level. In the rat, relative expression of the RNA species encoding the GHR and the GH-binding protein (GHBP) appears to be sensitive to endocrine status. Full-length GHR cDNA clones from ovine, porcine, and chicken were used as probes to investigate the existence of unique RNAs for GHBPs in these species. In the sheep and pig, only a single, approximately 4.5-kb RNA is apparent. Although quite high levels of GH binding activity are found in pig serum, a variety of methods failed to isolate a separate GHBP message, suggesting that porcine GHBP is produced via a mechanism different from that which is known for rat. One class of chicken GHR cDNA, resulting from alternative use of a splice acceptor 17 bases upstream of the intron 6/Exon 7 junction, is also presented.
Agricultural Research Division, American Cyanamid Company, Princeton, New Jersey 08543.
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Bingham
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Oldham
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Baumbach
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011986:Receptors, Somatotropin / Q000235:genetics*; D012756:Sheep; D013552:Swine
D002352:Carrier Proteins; D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D011986:Receptors, Somatotropin; C051815:somatotropin-binding protein