Subdivision of Salmonella enteritidis PT 4 by pulsed-field gel electrophoresis: potential for epidemiological surveillance.
Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to pulsed-field profile (PFP) 1 predominated. DNA-DNA hybridization studies demonstrated that a band of approximately 57 kb was associated with the presence of the 38 MDa S. enteritidis 'serovar-specific' plasmid. It is concluded that PFGE provides a method for discriminating strains of S. enteritidis PT 4 suitable for epidemiological investigations.
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
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Powell
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Threlfall
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Rowe
D000818:Animals; D002645:Chickens / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008460:Meat; D010957:Plasmids; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial