[Components of membranous labyrinth proteins of guinea pig and the detection of anti-68kD antibody in sensorineural hearing loss].
The membranous labyrinth proteins (MLP) of guinea pig were isolated with various methods. The isolations with SDS or Triton X-100 were efficient. Western blot was successfully established and used to detect the anti-MLP antibody. Among 42 cases of idiopathic sensorineural hearing loss, 16 are positive of anti-68kD antibody in their sera with increased IgG, IgM and IgA. Anti-68kD antibody was also found in some normal controls, though it had weaker reaction and less repetition.
Institute of Otorhinolaryngology of PLA, Beijing.
J
J
Zou
S
S
Zhai
P
P
Wang
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D015153:Blotting, Western; D006168:Guinea Pigs; D006319:Hearing Loss, Sensorineural / Q000276:immunology*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification
D001323:Autoantibodies; D008565:Membrane Proteins