Separation of closely related intrinsic membrane polypeptides of the photosystem II light-harvesting complex (LHC II) by reversed-phase high-performance liquid chromatography on a poly(styrene-divinylbenzene) column.
The three closely related intrinsic membrane polypeptides of the photosystem II light-harvesting complex (LHC II) were successfully resolved on a PRP-1 poly(styrene-divinylbenzene) column using a three-stage linear water-acetonitrile gradient containing 0.1% trifluoroacetic acid. The hydrophobic proteins of photosystem I (PS I-200) and photosystem II core particles were also separated by this method, showing that membrane proteins of different sizes and hydrophobicities can be resolved in this system.
Botany Department, University of British Columbia, Vancouver, Canada.
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Damm
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Green
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004591:Electrophoresis, Polyacrylamide Gel; D007475:Ion Exchange Resins; D008565:Membrane Proteins / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D011137:Polystyrenes; D013056:Spectrophotometry, Ultraviolet
D007475:Ion Exchange Resins; D008565:Membrane Proteins; D010455:Peptides; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D011137:Polystyrenes; C003771:Amberlite XAD-2 resin