A reliable method of embedding a small amount of dispersed cells for electron microscopy.
To embed a small amount of dispersed cells for electron microscopy, we used the supernatant of agarose solution which had been centrifuged beforehand. We also warmed the chamber of the centrifuge by placing a flexible ribbon heater at the bottom of the chamber to prevent the premature gelation of agarose. By these methods, as few as 10(6) dispersed cells can be reproducibly processed and embedded for electron microscopy.
Second Department of Anatomy, Kanazawa Medical University, Ishikawa, Japan.
Y
Y
Taniguchi
R
R
Tamatani
Y
Y
Kawarai
D002478:Cells, Cultured / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D012685:Sepharose; D016610:Tissue Embedding / Q000379:methods*
D012685:Sepharose