[The use of pyrophosphate-dependent phosphofructokinase for the determination of fructose-2,6-bisphosphate concentration in biological material].
The authors discuss methods for purification of pyrophosphate fructose-6-phosphate phosphotransferase from potato tuber and enzymatic method for measuring fructose-2,6-biphosphate concentration in isolated hepatocytes of normal and diabetic rats and come to a conclusion on a higher sensitivity of the method they propose in comparison with the method making use of commercial phosphofructokinase from rabbit muscles. Their method may be used for fructose-2,6-biphosphate measurements in various biologic materials.
M A
MA
Golubev
M S
MS
Markova
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004795:Enzyme Stability; D005635:Fructosediphosphates / Q000032:analysis*; D007202:Indicators and Reagents / Q000302:isolation & purification; D008099:Liver / Q000737:chemistry; D008722:Methods; D010770:Phosphotransferases / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D015203:Reproducibility of Results; D011198:Solanum tuberosum / Q000201:enzymology
D005635:Fructosediphosphates; D007202:Indicators and Reagents; C027652:fructose 2,6-diphosphate; D010770:Phosphotransferases; C022502:pyrophosphate-fructose 6-phosphate 1-phosphotransferase
Ispol'zovanie pirofosfatzavisimoĭ fosfofruktokinazy dlia opredeleniia kontsentratsii fruktozo-2,6-bisfosfata v biologicheskom materiale.