Nuclear localization of tumor necrosis factor-alpha in human osteoblast-like cells.
We have studied the internalization and subsequent nuclear localization of [125I]-rTNF-alpha in a human osteoblast-like cell line, HOS TE85. TNF-alpha is rapidly internalized and optimum cellular levels are achieved in approximately 20 minutes at 37 degrees C. A portion of the internalized cytokine is translocated to the nucleus as judged by its presence in highly purified nuclei as a 17-kDa form.
Department of Biochemistry and Molecular Biology, Graduate School of Biomedical Sciences, Newark, N.J.
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Panagakos
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Kumar
D000595:Amino Acid Sequence; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000378:metabolism*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha