Organization, sequence, and function of 34.5 kb of genomic DNA encompassing several murine T-cell receptor alpha/delta variable gene segments.
A cosmid-bearing murine genomic DNA, encompassing several T-cell receptor variable alpha/delta gene segments, has been sequenced using shotgun DNA cloning methodology coupled with fluorescence-based high-through-put DNA sequencing technology. This region, spanning 34.5 kb, contains a pseudogene V alpha gene segment (psi V alpha 16.1), a V delta gene segment (V delta 2), and a variable gene segment that has been reported to be expressed with both C alpha (V alpha 6) and C delta (V delta 3). Therefore, this cosmid is the ideal vehicle for examining the possible control sequences that may be involved in determining whether a V gene segment associates with either C alpha or C delta. Polymerase chain reaction experiments demonstrate that the two functional variable gene segments (V delta and V alpha/delta) are expressed individually with both C alpha and C delta genes as mRNA, indicating a permissiveness in their expression patterns. In addition, a variety of genomic sequence-related features have been identified.
Division of Biology (147-75), California Institute of Technology, Pasadena 91125.
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Koop
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Deshpande
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Howard
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Wilk
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Wang
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Hood
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D014644:Genetic Variation; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D004247:DNA