International validation study for the determination of chloramphenicol in bovine muscle.
A gas chromatographic (GC) procedure for the quantitation and GC/negative ion chemical ionization mass spectrometric (NICIMS) confirmation of chloramphenicol in calf muscle tissue was the subject of a validation study. Five analysts representing 5 laboratories in 4 countries participated in the quantitative method and analyzed 7 randomly numbered blind triplicates at 4 fortified and 3 incurred tissue concentrations on 3 separate days. The chloramphenicol concentrations ranged from 0 to 2.5 ppb. All data were reported to 3 significant figures. The coefficients of variation were 9.5-28.7% for repeatability and 14.6-38% over the study range for reproducibility. NICIMS data representing 3 laboratories in 3 countries successfully confirmed chloramphenicol in samples at 0.6 ppb or greater with no false positives in blank tissues.
Epstein
R L
RL
U.S. Department of Agriculture, Food Safety and Inspection Service, Washington, DC 20250.
Henry
C
C
Holland
K P
KP
Dreas
J
J
D000818:Animals; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis*; D002849:Chromatography, Gas / Q000706:statistics & numerical data*; D008401:Gas Chromatography-Mass Spectrometry / Q000706:statistics & numerical data*; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results
D002701:Chloramphenicol