Identification of a protein encoded by a mouse simple repeated sequence.
In order to study the possibility that a mouse repeated 'simple sequence', containing an ORF, could encode a protein, we inserted a fragment of a cDNA clone into the expression vector pEX31C. The fragment containing the short sequence 'CAGAGAGG' was expressed as MS2 polymerase fusion protein in Escherichia coli. This fusion protein (H5fp) was injected subcutaneously into rabbit and the corresponding polyclonal antibodies generated. Western blot analysis of proteins extracted from different mouse tissues established that anti H5fp antibodies recognized, in vivo, an antigen of 28 KDa. Immunolocalization with anti H5fp antibodies showed the presence of the related antigen in the cytoplasm of the examined sections. An hypothetical secondary structure for the protein was predicted by the Chou and Fasman method.
Di Carlo
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M
Institute of Developmental Biology, C.N.R., Palermo, Italy.
Romancino
D P
DP
Ghersi
G
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Montana
G
G
Monteleone
D
D
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli; D005822:Genetic Vectors; D007106:Immune Sera / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry; D008168:Lung / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009206:Myocardium / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000737:chemistry
D007106:Immune Sera; D011993:Recombinant Fusion Proteins; D004247:DNA