Sialylation and sulfation of the carbohydrate chains in respiratory mucins from a patient with cystic fibrosis.
Mucin glycopeptides were prepared from the sputum of a patient suffering from cystic fibrosis. The carbohydrate chains were released by alkaline borohydride treatment. Sialylated and sulfated oligosaccharide-alditols were purified by ion-exchange chromatography, gel filtration, and high performance anion-exchange chromatography. The structures of the oligosaccharide-alditols were determined by high resolution 1H NMR spectroscopy in combination with fast atom bombardment-mass spectrometry. Twenty-four oligosaccharides were characterized and illustrate the diversity of the carbohydrate chains of cystic fibrosis respiratory mucins. Among these 24 oligosaccharide-alditols, 15 are novel structures. Sialylation may occur on the C-6 of the N-acetylgalactosamine involved in the carbohydrate-peptide linkage or on a terminal galactose residue, either on C-3 or on C-6. Sulfation may occur either on the C-3 of a terminal galactose residue or on the C-6 of a N-acetylglucosamine residue. The most complex structures contain sulfated derivatives of the H,X, or Y determinants or a sialylated and sulfated derivative of the X determinant (Structure A).
Lo-Guidice
J M
JM
Unité INSERM No 377, Lille, France.
Wieruszeski
J M
JM
Lemoine
J
J
Verbert
A
A
Roussel
P
P
Lamblin
G
G
D002240:Carbohydrate Sequence; D002648:Child; D003550:Cystic Fibrosis / Q000378:metabolism*; D006020:Glycopeptides / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry*; D012794:Sialic Acids / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry
D006020:Glycopeptides; D009077:Mucins; D012794:Sialic Acids; D013431:Sulfates