Gene therapy for the treatment of malignant brain tumors with in vivo tumor transduction with the herpes simplex thymidine kinase gene/ganciclovir system.
Murine retroviral vectors can infect a wide variety of proliferating mammalian cell types (e.g. lymphocytes). Non-proliferating tissues (e.g. neurons) are not transduced by murine retroviral vectors. These findings suggest that this type of vector may be useful for the selective introduction of genes into growing tumors in the brain, since the tumor is essentially the only tissue that will integrate and express the vector genes.
Culver
K W
KW
Van Gilder
J
J
Link
C J
CJ
Carlstrom
T
T
Buroker
T
T
Yuh
W
W
Koch
K
K
Schabold
K
K
Doornbas
S
S
Wetjen
B
B
D000328:Adult; D000818:Animals; D001932:Brain Neoplasms / Q000097:blood / Q000134:cerebrospinal fluid / Q000188:drug therapy / Q000601:surgery / Q000628:therapy*; D002470:Cell Survival; D002478:Cells, Cultured / Q000382:microbiology / Q000637:transplantation; D002985:Clinical Protocols; D003131:Combined Modality Therapy; D005260:Female; D015774:Ganciclovir / Q000627:therapeutic use*; D015316:Genetic Therapy / Q000009:adverse effects; D005822:Genetic Vectors; D006801:Humans; D007258:Informed Consent; D007278:Injections, Spinal / Q000295:instrumentation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008979:Moloney murine leukemia virus / Q000235:genetics; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D016879:Salvage Therapy; D012513:Sarcoma, Experimental / Q000628:therapy; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics; D013937:Thymidine Kinase / Q000037:antagonists & inhibitors / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D013937:Thymidine Kinase; D015774:Ganciclovir