Improved detection of loss of heterozygosity at retinoblastoma gene locus in human breast carcinoma.
Loss of heterozygosity (LOH) at the retinoblastoma (Rb) gene locus was investigated in 33 breast carcinomas by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis after tumor cell enrichment by cell sorting. The efficacy of cell sorting was evaluated by comparing the results of PCR-SSCP with and without cell sorting. Ten of 17 (59%) informative cases showed LOH at this locus by cell sorting combined with PCR-SSCP, although LOH was detectable in six (35%) cases without cell sorting. Flow cytometry and histologic examination revealed that this underestimation may occur when the tumor cell population is less than 50% in the specimens analyzed. It is concluded that LOH of the Rb gene occurs more frequently in human breast carcinoma than previously thought, and thus may contribute significantly to the development and/or progression of this tumor.
Department of Pathology, Iwate Medical University School of Medicine, Morioka, Japan.
G
G
Tamura
C
C
Maesawa
Y
Y
Suzuki
M
M
Kashiwaba
M
M
Ishida
K
K
Saito
R
R
Satodate
D000230:Adenocarcinoma / Q000235:genetics / Q000556:secondary; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D005260:Female; D016161:Genes, Retinoblastoma / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D008207:Lymphatic Metastasis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded