The effect of allopurinol on Na+K+ATPase related lipid peroxidation in ischemic and reperfused rabbit kidney.
1. Na+K+ATPase is a membrane bound enzyme whose activity is essential for maintenance of cell viability. Lipid peroxidation changes membrane fluidity and enzyme activity. 2. The purpose of this study was to investigate the effect of allopurinol (free radical scavenger) on Na+K+ATPase activity in rabbit kidney cortex membrane. In this in vivo study we created ischemia and reperfusion in rabbit kidneys. 3. Enzyme activity were low in ischemic and reperfused kidneys, compared to the controls. In allopurinol treated ischemic and reperfused groups, the levels of Na+K+ATPase activity were high compared to the untreated group. 4. It has been concluded that allopurinol may protect this enzyme activity.
Department of Biochemistry, Medical Faculty, Gazi University, Ankara, Turkey.
A
A
Aricioğlu
S
S
Aydin
N
N
Turkozkan
O
O
Durmus
D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D066298:In Vitro Techniques; D007511:Ischemia / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D011817:Rabbits; D012079:Renal Circulation / Q000187:drug effects*; D015424:Reperfusion; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D010710:Phosphates; D008315:Malondialdehyde; D000493:Allopurinol; D000254:Sodium-Potassium-Exchanging ATPase