[Assisted hatching and micro-fertilization].
Erbium-YAG laser has been applied for micromanipulation in human beings. In a first series of attempts it was used in the fertilization process for subzonal insemination (SUZI): laser assisted micromanipulation achieved significant higher fertilization rates (34.8%) when compared to mechanical SUZI (16.1%), and for partial zona dissection (PZD): in this case laser did not improve the results (laser 14.8% vs mechanical 14%). Erbium-YAG laser was used to assist hatching. In the mouse it significantly improved the hatching rate (80% vs 29.3%) 110 hours post hCG. This technique was applied in two different centres to patients with previous IVF failures. The implantation rate per embryo (14.4% laser assisted hatching vs 6% control group) and the pregnancy rate per transfer (40% vs 16.2%) were improved.
Institute of Sterility Treatment, Vienna, Austria.
A
A
Obruca
H
H
Strohmer
D
D
Sakkas
Y
Y
Menezo
A
A
Kogosowski
Y
Y
Barak
W
W
Feichtinger
D000818:Animals; D004195:Disease Models, Animal; D010064:Embryo Implantation; D005260:Female; D006801:Humans; D007315:Insemination, Artificial / Q000379:methods*; D007834:Lasers; D051379:Mice; D008845:Microinjections / Q000379:methods*; D011247:Pregnancy; D011256:Pregnancy Outcome; D017211:Treatment Failure; D015044:Zona Pellucida
Eclosion et microfécondation assistées.