IgE-specific unresponsiveness in mice induced by ovalbumin coupled with murine red blood cells.
Ovalbumin (OVA) was coupled with murine red blood cells (MRBC) using glutaraldehyde. OVA-MRBC conjugate induced anti-OVA IgG antibody production in mice at almost the same level as OVA in alum. However, no IgE antibody production specific for OVA was observed in OVA-MRBC-injected mice. IgE-specific unresponsiveness was also observed in mice injected with OVA coupled with sheep red blood cells (OVA-SRBC), suggesting that our present observation was not restricted to the property of MRBC. These results show the potential ability of antigen-RBC conjugate for the development of vaccine that induces sufficient IgG antibody production without IgE synthesis.
Department of Safety Research on Biologics, National Institute of Health, Tokyo, Japan.
T
T
Uchida
A
A
Horino
S
S
Naito
J
J
Mizuguchi
D000534:Alum Compounds; D000818:Animals; D004912:Erythrocytes / Q000276:immunology* / Q000378:metabolism; D005260:Female; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000534:Alum Compounds; D007074:Immunoglobulin G; C041524:aluminum sulfate; D007073:Immunoglobulin E; D010047:Ovalbumin