[A cell-free translation system based on mollicute ribosomes].
Active cell-free protein-synthesizing systems are created on the basis of ribosomes of Acholeplasma laidlawii var. granulum str. 118, A. laidlawii PG-8 and active lysates of eucaryotic cells (lysate of rabbit reticulocytes and wheat germ extract) in which activity of 80S-ribosomes was suppressed by addition of actidione in concentrations of 150 and 200 mkg/ml, respectively. It is shown that preincubation of active lysates of eucaryotic cells with actidione does not influence their ribosome inhibition level. It was found that the system of in vitro translation on the basis of wheat germ extract was more specific for ribosomes of phytopathogenic acholeplasmas (str. 118), while ribosomes of the saprophytic strain (PG-8) revealed no such specificity with respect to cell-free translation systems.
Korobkova
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D000128:Acholeplasma laidlawii / Q000187:drug effects / Q000378:metabolism*; D002458:Cell Fractionation; D003513:Cycloheximide / Q000494:pharmacology; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D014176:Protein Biosynthesis / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*
D003513:Cycloheximide
Beskletochnaia sistema transliatsii na osnove ribosom mollikutov.