Cloning, nucleotide sequence and characterization of a gene encoding superoxide dismutase from Campylobacter jejuni and Campylobacter coli.
Genes encoding superoxide dismutase (SOD: EC 1.15.1.1) were cloned from Campylobacter jejuni NCTC 11351 and Campylobacter coli UA585 by heterologous complementation of a SOD-deficient Escherichia coli mutant. Deletion analysis of the cloned C. jejuni DNA assigned the sod gene to a 1.2 kb insert and this contained an open reading frame of 660 bp. The deduced gene product of 220 amino acids was 71% identical to the E. coli iron-containing SOD and 60% identical to the E. coli manganese-containing SOD. The recombinant SOD was expressed at high levels in E. coli and protected a sodA sodB double mutant from the toxic effects of methyl viologen. Nucleotide sequence analysis of the corresponding gene from C. coli showed it to be 92% identical to that from C. jejuni.
Purdy
D
D
Institute of Food Research, Reading Laboratory, UK.
Park
S F
SF
D000595:Amino Acid Sequence; D001483:Base Sequence; D017000:Campylobacter coli / Q000201:enzymology / Q000235:genetics*; D016123:Campylobacter jejuni / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*
D011994:Recombinant Proteins; D013482:Superoxide Dismutase