Oestrogen and progesterone do not regulate the expression of retinoic acid receptors and retinoid 'X' receptors in human endometrial stromal cells in vitro.
Elucidation of the gene structure for retinoic acid receptor-beta (RAR-beta) has suggested a potential role for oestrogen in regulating the expression of RAR-beta. We have previously shown that all three RAR types are expressed in human endometrial stromal cells in vitro and that RAR-beta expression is induced in response to retinoic acid. The aim of this study was to ask whether oestradiol and progesterone could play a part in regulating the expression of RARs in human endometrial stromal cells and to establish the patterns of expression of a related group of nuclear retinoid receptors, retinoid 'X' receptors (RXRs) and their potential for regulation by steroid hormones. The RAR expression patterns of endometrial stromal cells, grown in steroid-free medium, did not change in response to the presence of steroid hormones. Furthermore, the retinoic acid-mediated induction of RAR-beta was not affected by oestradiol or progesterone, and was dependent on the continued presence of retinoic acid. Of the three RXR types, only RXR-alpha was detectably expressed in stromal cells in vitro and the expression of RXR-alpha did not change in response to steroid hormones or retinoic acid. These data indicate that oestradiol and progesterone are not important in the regulation of RAR and RXR expression in human endometrial stromal cells.
Department of Obstetrics & Gynaecology, Medical School, University of Newcastle, Newcastle upon Tyne, UK.
M K
MK
Kumarendran
C J
CJ
Matthews
M D
MD
Levasseur
A
A
Prentice
E J
EJ
Thomas
C P
CP
Redfern
D001483:Base Sequence; D002478:Cells, Cultured; D004717:Endometrium / Q000378:metabolism*; D004967:Estrogens / Q000502:physiology*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D011374:Progesterone / Q000502:physiology*; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis*; D018168:Receptors, Retinoic Acid / Q000096:biosynthesis*; D047488:Retinoid X Receptors; D017154:Stromal Cells / Q000378:metabolism; D014157:Transcription Factors
D004967:Estrogens; D009687:Nuclear Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D014157:Transcription Factors; D011374:Progesterone