A combined study of human complement C7 IEF and C7 M/N polymorphisms in the Chinese Han population.
Two polymorphisms of human complement C7 (IEF and M/N) have been studied in the Han population from Chengdu, People's Republic of China. One was determined by polyacrylamide gel isoelectric focusing (IEF) of neuraminidase-treated EDTA plasma, whereas the other was performed by a combination of two ELISAs using a C7-allospecific monoclonal antibody or polyclonal C7-specific IgG as coating antibodies. In 121 Chinese subjects IEF patterns of C7 were classified into three common and four rare phenotypes. The C7 IEF allele frequency estimates for C7*1, C7*2, C7*3, C7*4 and C7*7 were 0.831, 0.095, 0.045, 0.025 and 0.004, respectively. The C7 M/N typing in 113 Chinese individuals revealed the following allele frequencies: C7*M = 0.805, C7*N = 0.195. Allelic associations between C7 IEF alleles and the C7 M/N alleles are also discussed.
Zhang
L
L
Institut für Rechtsmedizin, Johannes-Gutenberg-Universität, Mainz, FRG.
Würzner
R
R
Stradmann-Bellinghausen
B
B
Rittner
C
C
Schneider
P M
PM
D044466:Asian People / Q000235:genetics*; D003184:Complement C7 / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*
D003184:Complement C7