The application of electrospray and neutral-loss mass spectrometry to the identification of the metabolites of S12813 in rat liver slices.
The metabolism of S12813, (3-(2-[4-phenyl piperazin-1-yl] ethyl)-2-oxo-2,3-dihydro oxazolo [4,5-b] pyridine chlorohydrate), in rat liver slice incubates was examined by high performance liquid chromatography combined with mass spectrometry. Electrospray ionization was used together with tandem mass spectrometric techniques of analysis (MS/MS). Polar phase I and phase II metabolites were identified as C-oxidation products, which were then conjugated to form either sulphate or glucuronide metabolites. On the basis of the identifications made, a metabolic pathway of S12813 in rat liver slices has been proposed.
Servier Research and Development Ltd., Slough, UK.
R
R
Brownsill
J P
JP
Combal
A
A
Taylor
M
M
Bertrand
W
W
Luijten
B
B
Walther
D000700:Analgesics / Q000493:pharmacokinetics*; D000818:Animals; D001711:Biotransformation; D002853:Chromatography, Liquid; D005965:Glucuronates / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D010080:Oxazoles / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013431:Sulfates / Q000378:metabolism
D000700:Analgesics; D005965:Glucuronates; D010080:Oxazoles; D013431:Sulfates; C088312:S 12813