Monitoring beta cell responses in obese and normal weight subjects--a pilot study.
Six obese and six normal subjects were given a test meal after an overnight fast. Blood glucose, plasma immunoreactive C peptide, immunoreactive insulin and proinsulin plus its circulating fragment des Lys64, Arg65 proinsulin were measured. Responses to the test meal were not significantly different for immunoreactive C peptide and insulin concentrations. Plasma insulin responses were similar in the two groups. Plasma C-peptide values showed wide variations in the obese subjects. Plasma proinsulin and proinsulin fragment levels rose slightly in the normal subjects reaching a peak level of 28.9 +/- 6.4 pmol/l whereas in the obese subjects these same levels rose to a maximum of 88 +/- 45 pmol/l. However because of the large intra group variation, differences were not significant.
School of Biological Sciences, University of Surrey, Guildford, England.
S M
SM
Hampton
L
L
Withey
D000328:Adult; D002096:C-Peptide / Q000097:blood; D004435:Eating / Q000502:physiology*; D006801:Humans; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000378:metabolism*; D008875:Middle Aged; D008991:Monitoring, Physiologic; D009765:Obesity / Q000097:blood / Q000503:physiopathology*; D010446:Peptide Fragments / Q000097:blood; D010865:Pilot Projects; D011384:Proinsulin / Q000097:blood; D012016:Reference Values
D002096:C-Peptide; D007328:Insulin; D010446:Peptide Fragments; C088305:proinsulin, des(Lys(64),Arg(65)); D011384:Proinsulin