Introduction of hepatitis delta virus into animal cell lines via cationic liposomes.
Cationic liposomes are known to facilitate efficient transfection of animal cells with DNA and even some viruses. As reported here, we have been able to use such a commercially available formulation (Lipofectamine) and introduce human hepatitis delta virus (HDV) into lines of cultured cells and demonstrate replication of the HDV genome both by immunofluorescence and by Northern (RNA) analysis. As much as 10% of the human hepatoma cell line Huh7 was transfected with HDV. Also transfected were the baby hamster kidney cell line BHK-21 and the Morris rat hepatoma line 7777. Two initial applications of HDV transfection have been made. (i) The ribonucleoprotein structure of HDV was isolated from disrupted virions and demonstrated as being sufficient to transfect Huh7 cells. In contrast, naked HDV RNA was not sufficient. (ii) From a study of cells transfected with HDV particles, it was found that, even after as long as 7 weeks and the associated replication of the transfected cells, the HDV RNA genome was still replicating. Apparently, HDV, in the absence of helper virus and in the absence of virus assembly, can maintain persistent replication and expression of the HDV genome. Transfection was also achieved with woodchuck hepatitis virus introduced into Huh7 cells. In summary, this transfection procedure should be of use for the study of these and maybe other recalcitrant animal viruses.
Bichko
V
V
Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111-2497.
Netter
H J
HJ
Taylor
J
J
D000818:Animals; D015152:Blotting, Northern; D002411:Cation Exchange Resins; D002412:Cations; D002460:Cell Line; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D017919:Hepatitis B Virus, Woodchuck / Q000502:physiology; D003698:Hepatitis Delta Virus / Q000502:physiology*; D006801:Humans; D008055:Lipids; D008081:Liposomes / Q000737:chemistry; D051381:Rats; D014162:Transfection; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D002411:Cation Exchange Resins; D002412:Cations; D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes