[Study on the role of EB virus in carcinogenesis of nasopharyngeal carcinoma by use of in situ hybridization].
Cryosections of 45 nasopharyngeal carcinomas and 9 non-tumorous biopsies were studied for EBV DNA by use of in situ hybridization. No EBV DNA was detected in all types of non-epithelial cells. One to three copies of EBV genome as shown by Namalwa cells could occasionally be found in a small number of normal, hyperplastic, squamously metaplastic and dysplastic epithelial cells. A considerable number of neoplastic cells in 6 para-tumourous carcinoma in situ foci contained numerous EBV genome copies as shown by Raji cells. The majority of infiltrating cancer cells of 42 non-keratinizing carcinomas and 3 keratinizing squamous cell carcinomas also exhibited quite a number of EBV genome copies. The above findings illustrate that EBV may have a promotive effect during the early stage just after the malignant conversion of epithelial cells to increase the proliferative rate of carcinoma cells.
Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou.
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D002294:Carcinoma, Squamous Cell / Q000382:microbiology* / Q000473:pathology; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000032:analysis; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology* / Q000473:pathology
D004279:DNA, Viral