A simplified method for large-scale HPA-1a phenotyping for antenatal screening.
A simplified method for large-scale HPA-1a phenotyping of platelets was developed for use in an antenatal screening programme for fetomaternal alloimmune thrombocytopenia (FMAIT). The test was based on the MAIPA assay, which was modified for antigen-typing with a well-characterized anti-HPA-1a reagent. The resulting assay gave reliable results, was inexpensive and allowed testing of large batches using semiautomated equipment.
Department of Haematology, St Bartholomew's Hospital and Medical College, London, U.K.
P
P
Metcalfe
H A
HA
Doughty
M F
MF
Murphy
A H
AH
Waters
D000911:Antibodies, Monoclonal / Q000276:immunology; D016824:Antigens, Human Platelet / Q000032:analysis* / Q000235:genetics; D016208:Databases, Factual; D005260:Female; D005820:Genetic Testing / Q000191:economics; D006801:Humans; D007124:Immunoenzyme Techniques; D039661:Integrin beta3; D010641:Phenotype; D011247:Pregnancy / Q000097:blood* / Q000276:immunology; D012699:Serology; D013921:Thrombocytopenia / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3