Studies on pharmacological activation of human immunoglobulin G by chemical modification and active subfragments. XII. Effect of carboxamidemethylated Fc fragment (CM-Fc) from human immunoglobulin G on mixed lymphocyte reaction.
The effect of carboxamidemethylated Fc fragment (CM-Fc) from human serum immunoglobulin G on mixed lymphocyte reaction (MLR) was studied. CM-Fc enhanced the MLR response when added at the start of cultivation, but suppressed the response when added 48 h after onset of cultivation. The Fc fragment had no effect. CM-Fc elevated the levels of interleukin-1 and tumor necrosis factor when added at the start of splenocyte cultivation, and somewhat did so when added 48 h after onset of cultivation. These elevations caused the induction of prostaglandin E2 in MLR culture supernatant. The suppression of MLR by CM-Fc was abrogated by the addition of indomethacin. We considered that CM-Fc regulated the immune response being affected to the function of macrophages.
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
T
T
Mimura
M
M
Itoh
T
T
Sugiyama
N
N
Suzuki
T
T
Nakanishi
K
K
Tsujikawa
Y
Y
Kohama
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone; D007213:Indomethacin