Protein kinase C rapidly up-regulates the number of AT1 angiotensin receptors on cultured rat intestinal epithelial (RIE-1) cells.
Addition of 12-O-tetradecanoylphorbol-13-acetate to RIE-1 rat intestinal epithelial cells stimulated a rapid (mean 3-fold) increase in the subsequent binding of 125I-labelled angiotensin II which was reversed or prevented when cellular protein kinase C was depleted. The increased binding was due, in part, to an up-regulation in the number of AT1 angiotensin receptors on RIE-1 cells, without any significant change in their binding affinity. Since this rapid up-regulation was independent of receptor synthesis, it may result from an increased availability (to extracellular ligand) of performed, but previously 'cryptic', AT1 angiotensin receptors.
Department of Clinical Biochemistry, Addenbrooke's Hospital, Cambridge, UK.
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D000818:Animals; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011945:Receptors, Angiotensin / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015854:Up-Regulation
D011945:Receptors, Angiotensin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate