Interindividual variability of the human hepatic sulphotransferases.
The variability among subjects of the hepatic activities of O-sulphotransferase towards dopamine, p-nitrophenol, testosterone and ethinyloestradiol and of N-sulphotransferase with 1,2,3,4-tetrahydroisoquinoline (TIQ) as substrate is described. The rates of testosterone and TIQ sulphation were higher in men than women whereas those of ethinyloestradiol, dopamine and p-nitrophenol were similar in both sexes. The sulphotransferase activities towards p-nitrophenol and dopamine were positively skewed whereas those towards ethinyloestradiol approached normality. The coefficients of variations for the sulphotransferase activities ranged between 34% and 62% indicating a considerable variability among subjects. The rates of dopamine-, TIQ- and p-nitrophenol-sulphation were measured in the mucosa of the human intestine, and the duodenum/liver ratios were 10, 0.9 and 0.1, respectively. Thus the contribution of the intestine in the sulphation of xenobiotics is substrate dependent.
Department of Biomedicine, Medical School, University of Pisa, Italy.
G M
GM
Pacifici
A
A
Temellini
M
M
Castiglioni
C
C
D'Alessandro
A
A
Ducci
L
L
Giuliani
D000328:Adult; D000368:Aged; D015239:Arylsulfotransferase / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D007546:Isoquinolines / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D012727:Sex Characteristics; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000378:metabolism*; D044005:Tetrahydroisoquinolines
D007527:Isoenzymes; D007546:Isoquinolines; D044005:Tetrahydroisoquinolines; D004997:Ethinyl Estradiol; C014843:1,2,3,4-tetrahydroisoquinoline; D015238:Sulfotransferases; C084556:ethinylestradiol-17 alpha sulfotransferase; C087860:testosterone sulfotransferase; D015239:Arylsulfotransferase; D004298:Dopamine