The effect of lactogenic hormones on protein synthesis and amino acid uptake in rat mammary acinar cell culture at various physiological stages.
1. The activities of protein synthesis and amino acid uptake at various physiological stages were determined by the incorporation of radioactive materials ([3H]-lysine, [14C]-cycloleucine) in rat mammary epithelial cell cultures. The activity of protein synthesis and amino acid uptake was higher in early lactation than in virgin, pregnant and late lactation stages. 2. Lactogenic hormones (prolactin, hydrocortisone and insulin) treatment related with mammary growth and differentiation increased the activities of protein synthesis and amino acid uptake. But increase of these activities was different at each physiological stage. 3. The effect of prolactin and hydrocortisone on the activities were greater in virgin, pregnant and late lactation than in early lactation. And effect of insulin was greater in pregnant and early lactation than in virgin and weanling.
Department of Animal Science and Technology, College of Agricultural and Life Sciences, Seoul National University, Republic of Korea.
S G
SG
Roh
M G
MG
Baik
Y J
YJ
Choi
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006728:Hormones / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007774:Lactation / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology; D014176:Protein Biosynthesis; D051381:Rats; D013997:Time Factors
D000596:Amino Acids; D006728:Hormones; D007328:Insulin; D011388:Prolactin; D006854:Hydrocortisone