Effects of spermine on formation of HGPRT- mutants induced by ethylmethanesulfonate, methylmethanesulfonate, and mitomycin C in V79 Chinese hamster cells.
Spermine is a polyamine found in bacteria, animal, and plant tissues. It is involved in a variety of biological processes, and its interaction with DNA stabilizes the secondary structure of the double helix. Spermine is one of the first reported antimutagens, reducing the mutation rate in several prokaryotic test systems, while in eukaryotic organisms conflicting results have been obtained. In light of the significant antimutagenic effect of spermine, it is important to evaluate its activity in mammalian cells in culture. The present study was undertaken to evaluate the ability of spermine to suppress the level of HGPRT- mutants induced by ethylmethanesulfonate, methylmethanesulfonate, and mitomycin C. Spermine reduced the mutation frequency induced by ethylmethanesulfonate and methylmethanesulfonate but did not affect survival; with mitomycin C survival was reduced but mutation rate was not influenced.
Istituto di Mutagenesi e Differenziamento, C.N.R., Pisa, Italy.
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D000478:Alkylation / Q000187:drug effects; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001667:Binding, Competitive; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005978:Glutathione / Q000032:analysis; D005982:Glutathione Transferase / Q000378:metabolism; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008168:Lung / Q000166:cytology / Q000187:drug effects; D008741:Methyl Methanesulfonate / Q000633:toxicity; D016685:Mitomycin / Q000633:toxicity; D016296:Mutagenesis / Q000187:drug effects*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D013096:Spermine / Q000494:pharmacology*
D016587:Antimutagenic Agents; D009153:Mutagens; D013096:Spermine; D016685:Mitomycin; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate; D007041:Hypoxanthine Phosphoribosyltransferase; D005982:Glutathione Transferase; D005978:Glutathione