Directed cell movement in pulsed electric fields.
Human granulocytes exposed to pulsed electric guiding fields were investigated. The trajectories were determined from digitized pictures (phase contrast). The basic results are: (i) No directed response was induced by pulsed electric guiding fields having a zero averaged field. (ii) A directed response was induced by pulsed electric guiding fields having a non-zero averaged field. (iii) The directed response was enhanced for pulse sequences having a repetition time of 8 s. (iv) The lag-time between signal recognition and cellular response was 8-10 s. The results are discussed in the framework of a self-ignition model.
Abteilung Biophysik, Universität Ulm, Bundesrepublik Deutschland.
K
K
Franke
H
H
Gruler
D002197:Capillary Action; D002634:Chemotaxis, Leukocyte; D004558:Electric Stimulation / Q000379:methods*; D006098:Granulocytes / Q000502:physiology*; D066298:In Vitro Techniques; D008433:Mathematics; D008954:Models, Biological; D013997:Time Factors