Molecular cloning of a novel human cDNA homologous to CDC10 in Saccharomyces cerevisiae.
We isolated a novel human cDNA, termed hCDC10, whose predicted product showed a high degree of homology to the CDC10 protein of Saccharomyces cerevisiae. This cDNA contained an open reading frame of 1254 nucleotides encoding 418 amino acids, which included a GTP-binding motif, GX4GKS--DX2G--KXD. The predicted peptide sequence also revealed partial amino-acid identity (40-50%) with Diff 6 in Drosophila and with H5 in mouse. Each of these sequence homologues, including Saccharomyces cerevisiae CDC10, contains the GTP-binding motif. Northern blot analyses indicated that the hCDC10 gene is expressed ubiquitously in normal tissues.
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
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Nakatsuru
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Sudo
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Nakamura
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila / Q000378:metabolism; D005260:Female; D005333:Fetus; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins / Q000737:chemistry*; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D058112:Septins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics*
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D005656:Fungal Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C061178:cdc10 protein, S pombe; C512892:CDC10 protein, S cerevisiae; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C512895:Sept7 protein, mouse; D058112:Septins