A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate.
A new method is reported for the incorporation of hydrophobic peptides into sodium dodecyl sulphate (SDS) micelles. First, a homogeneous solution of peptide and detergent is obtained by adding the peptide in trifluoroethanol to an equal volume of an aqueous solution of SDS. Upon subsequent addition of excess water, mixed peptide-SDS micelles are formed. Next, all solvent is removed by lyophilization and an appropriate amount of water is added to the dry powder. For various hydrophobic peptides this was shown to yield clear and stable solutions that are highly concentrated and suitable for characterization by spectroscopic techniques.
Killian
J A
JA
Department of Biochemistry of Membranes, University of Utrecht, The Netherlands.
Trouard
T P
TP
Greathouse
D V
DV
Chupin
V
V
Lindblom
G
G
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D008823:Micelles; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*
D008823:Micelles; D010455:Peptides; D012967:Sodium Dodecyl Sulfate