Hematoporphyrin derivative-induced photodynamic inhibition of Na+/K(+)-ATPase in L929 fibroblasts, Chinese hamster ovary cells and T24 human bladder transitional carcinoma cells.
The photodynamically induced inhibition of Na+/K(+)-ATPase with hematoporphyrin derivative as photosensitizer was studied in murine L929 fibroblasts, Chinese hamster ovary (CHO) cells and T24 human bladder transitional carcinoma cells. In T24 cells the inhibition of the enzyme activity appeared to be caused by ATP depletion rather than by direct damage from the enzyme itself. In L929 and CHO cells, on the other hand, the inhibition was caused by photodynamic damage from the enzyme molecule. For all three cell lines it was shown that a causal relationship between photodynamically induced reduction in Na+/K(+)-ATPase activity and the loss of clonogenicity is highly unlikely.
Sylvius Laboratory, Department of Medical Biochemistry, Leiden, The Netherlands.
L C
LC
Penning
K
K
Tijssen
J
J
VanSteveninck
T M
TM
Dubbelman
D000818:Animals; D016466:CHO Cells / Q000187:drug effects / Q000201:enzymology; D002460:Cell Line; D006224:Cricetinae; D017324:Hematoporphyrin Derivative / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D010778:Photochemotherapy; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D017324:Hematoporphyrin Derivative; D000254:Sodium-Potassium-Exchanging ATPase