Dehalogenation by Mycobacterium vaccae JOB-5: role of the propane monooxygenase.
Mycobacterium vaccae JOB-5 has an inducible propane monooxygenase that has been implicated in the catabolism of most major groundwater pollutants including trichloroethylene. Propane-grown cells are also induced for the dehalogenation of 1-chlorobutane and other chloroalkanes. 1-Chlorobutane is oxidized to 2-butanol, indicating that subterminal oxidation of 1-chlorobutane resulted in a concomitant release of the chloride. Nonproliferating suspensions of M. vaccae induced for the propane monooxygenase can dehalogenate a variety of chlorinated hydrocarbons including monochlorinated alcohols, dichlorinated short chain alkanes, and several multiple-substituted compounds including trichloroethylene. The results indicate that M. vaccae JOB-5 has a monooxygenase of broad specificity that can dehalogenate an array of halogenated hydrocarbons.
Department of Microbiology, North Carolina State University, Raleigh 27695-7615.
L A
LA
Vanderberg
J J
JJ
Perry
D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry; D004790:Enzyme Induction; D006846:Hydrocarbons, Halogenated / Q000378:metabolism*; D009161:Mycobacterium / Q000201:enzymology / Q000378:metabolism*; D010105:Oxygenases / Q000096:biosynthesis / Q000378:metabolism; D011407:Propane
D003470:Culture Media; D006846:Hydrocarbons, Halogenated; D010105:Oxygenases; D011407:Propane