Cytogenetic analysis of murine cell lines from diethylstilbestrol-induced uterine endometrial adenocarcinomas.
Treatment of female CD-1 mice with the synthetic estrogen diethylstilbestrol (DES) on days 1 through 5 after birth results in a 90% incidence of endometrial adenocarcinomas by 18 months of age Three cell lines were established from DES-induced uterine carcinomas and studied for specific chromosomal changes. Each cell line exhibited numerical decreases in chromosomes 9, 11, 13, and X as common abnormalities. Structural alterations involving chromosomes 3, 6, 11, and 19 occurred nonrandomly among the three cell lines. Every cell line showed a rearrangement in the long arm of chromosome 3 (3q+), a translocation between chromosomes 3 and 19 [t(3;19)], isochromosome of chromosome 11 [i(11)], and a marker chromosome (M2) either as common abnormalities or recurrent abnormalities. t(3;19), i(11), and M2 were observed also in the primary colonies from which the cell lines arose. The changes were not observed in a cell line derived from the uterus of one untreated control mouse, suggesting that these chromosomal alterations may have occurred during DES-induced neoplastic transformation. The chromosomal alterations found in the present study may prove useful in investigating the genetic changes involved in DES carcinogenesis.
Laboratory of Reproductive and Development Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
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Endo
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Kodama
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Newbold
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McLachlan
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Barrett
D000230:Adenocarcinoma / Q000139:chemically induced* / Q000235:genetics*; D000782:Aneuploidy; D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002869:Chromosome Aberrations; D004054:Diethylstilbestrol / Q000009:adverse effects*; D005260:Female; D007621:Karyotyping; D051379:Mice; D014178:Translocation, Genetic; D014594:Uterine Neoplasms / Q000139:chemically induced* / Q000235:genetics*
D004054:Diethylstilbestrol