An electrochemical method for detection of nucleic acid hybridisation.
Preliminary investigations on the detection of nucleic acid hybridisation by direct electrochemical techniques are described. Initial experiments were performed with calf thymus DNA, while as a model system, plasmid DNA (pEMBL) which can be prepared in single stranded forms from each of two original double stranded plasmids (+19 and -19) was used. Electrochemical analyses have been performed using mercury pool, glassy carbon and screen printed carbon working electrodes.
Cranfield Biotechnology Centre, Cranfield University, Bedfordshire.
J M
JM
Hall
J
J
Moore-Smith
J V
JV
Bannister
I J
IJ
Higgins
D000818:Animals; D015374:Biosensing Techniques; D002138:Calibration; D002244:Carbon; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D009693:Nucleic Acid Hybridization; D013950:Thymus Gland / Q000737:chemistry
D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D002244:Carbon