The effects of a 0.3% triclosan-containing dentifrice on the microbial composition of supragingival plaque.
144 subjects completed a 6-month, double-blind study which examined the effects of a 0.3% triclosan/2% copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. The subjects were randomly assigned to use, in an oral hygiene program, either the triclosan/copolymer/fluoride test dentifrice or a control dentifrice. The latter had the same formulation as the test dentifrice except it did not contain triclosan. Supragingival plaque was collected from the buccal and lingual surfaces of 4 teeth at baseline, 3 months, and 6 months, and microbiologically examined by darkfield microscopy, gram stain morphology, immunofluorescence, and selective and non-selective media. Antimicrobial susceptibilities were determined by agar dilution and whole plaque susceptibility methodologies on plaque samples from 136 subjects at each of the above sample periods and at 6-week intervals for an additional 6 months post-therapy. Both dentifrices resulted in highly statistically significant reductions in the total cultivable flora obtained at both the 3 and 6-month samples relative to baseline as well as at 6 months relative to the 3-month sample. The relative decrease in total anaerobic counts and in strict anaerobes, while not statistically significant, was more pronounced at both the 3- and 6-month sample periods in subjects receiving the triclosan dentifrice than for the controls. Neither dentifrice resulted in detrimental shifts in the microbial composition of the normal flora nor led to the emergence of periodontal or opportunistic pathogens.(ABSTRACT TRUNCATED AT 250 WORDS)
Periodontal Disease Research Center, University of Florida, Gainesville.
C
C
Walker
L C
LC
Borden
J J
JJ
Zambon
C Y
CY
Bonta
W
W
DeVizio
A R
AR
Volpe
D000190:Actinomyces / Q000187:drug effects; D000704:Analysis of Variance; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000382:microbiology*; D003802:Dentifrices / Q000494:pharmacology*; D004311:Double-Blind Method; D004352:Drug Resistance, Microbial; D004463:Ecology; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D008137:Longitudinal Studies; D008826:Microbial Sensitivity Tests; D014260:Triclosan / Q000494:pharmacology*; D014678:Veillonella / Q000187:drug effects
D003802:Dentifrices; D014260:Triclosan