[The introduction of the marker gene Neor into hematopoietic stem cells by electroporation].
An electroporation method has been used to introduce marker gene Neor into mouse stem hemopoietic cells which are capable of long-term hemopoiesis maintenance in marrow long-term cultures. Integration of the gene was tested by polymerase chain reaction. The effect of the procedure averaged 50-80% of marked CFUc. Electroporation did little damage to hemopoietic cell precursors. Gene transfer can be made most effectively using bone marrow from mice injected 5-fluorouracil 4 days prior to the experiment.
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NI
Drize
O I
OI
Gan
V L
VL
Surin
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D003114:Colony-Forming Units Assay; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D018014:Gene Transfer Techniques; D005819:Genetic Markers / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010957:Plasmids / Q000235:genetics; D013997:Time Factors
D005819:Genetic Markers; D005472:Fluorouracil
Vvedenie markernogo gena Neor v stvolovye krovetvornye kletki metodom élektroporatsii.