Purification and characterization of the U-particle, a cellular constituent whose synthesis is stimulated by Mengovirus infection.
We have isolated a cellular protein particle whose synthesis is induced by infection with Mengovirus or TMEV. The U-particle inhibits translation in vitro and binds to both capped and uncapped mRNA's. It is spherical, 12 nm in diameter, and is composed of multiple copies of two polypeptide subunits having molecular weights of 23,000 and 25,000 which do not appear to be glycosylated or phosphorylated. U-particles are capable of inhibiting mRNA translation in vitro.
Department of Biochemistry, University of Alberta, Edmonton, Canada.
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Mulvey
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Fang
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Scraba
D000818:Animals; D006801:Humans; D007181:Inclusion Bodies, Viral / Q000737:chemistry* / Q000648:ultrastructure; D004680:Maus Elberfeld virus / Q000254:growth & development; D008574:Mengovirus / Q000254:growth & development*; D051379:Mice; D011485:Protein Binding; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000302:isolation & purification* / Q000648:ultrastructure
D012333:RNA, Messenger; D016601:RNA-Binding Proteins