Effects of protein kinase inhibitors on the cell motility stimulated by autocrine motility factor.
Preincubation of Dunn osteosarcoma cells for 1 h with both 100 nM of staurosporine and 10 micrograms/ml of genistein resulted in a significant decrease in the motility stimulated by autocrine motility factor (AMF), whereas these reagents did not affect the basal motility and proliferation at these concentrations. The effect of the agents on the stimulated motility was both dose- and time-dependent. The motility stimulated by the anti-AMF receptor mAb was also inhibited. In contrast, H-8 had a negligible effect upon the stimulated motility. These data suggest that both kinase C and tyrosine kinase play a role in AMF-stimulated cell motility, while protein kinase A, which is selectively associated with the adenylate cyclase pathway, may not be required for the stimulation.
Department of Orthopedic Surgery, Gunma University School of Medicine, Japan.
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Kanbe
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Chigira
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Watanabe
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D019833:Genistein; D005956:Glucose-6-Phosphate Isomerase / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007529:Isoflavones / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors; D019311:Staurosporine; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D000911:Antibodies, Monoclonal; D007529:Isoflavones; D007546:Isoquinolines; D047428:Protein Kinase Inhibitors; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; D019833:Genistein; D005956:Glucose-6-Phosphate Isomerase; D019311:Staurosporine