Protein tyrosine phosphatase activity enhancement is induced upon Fc epsilon receptor activation of mast cells.
Immunological stimulation of rat mucosal type mast cells (line RBL-2H3) by clustering the type I Fc epsilon receptor (Fc epsilon RI) causes a fast and transient tyrosine phosphorylation of several proteins. This implied the involvement of both, protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPases) in that process. In order to identify the PTPases involved in these very early steps coupling Fc epsilon RI stimulus to cell response, we undertook the purification and characterization of PTPases present in RBL-2H3 cells. In one of the cells' membranal fractions, a PTPase activity was found to be enhanced 2- to 3-fold upon cell stimulation by Fc epsilon RI clustering. Characterization of this activity implies its involvement in control of the Fc epsilon RI signalling cascade.
Hampe
C S
CS
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Pecht
I
I
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D004140:Dinitrophenols / Q000276:immunology; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007073:Immunoglobulin E / Q000276:immunology; D007700:Kinetics; D008407:Mast Cells / Q000201:enzymology* / Q000276:immunology; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011961:Receptors, Fc / Q000502:physiology*; D012710:Serum Albumin, Bovine / Q000276:immunology; D013379:Substrate Specificity
D004140:Dinitrophenols; D011961:Receptors, Fc; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E; D017027:Protein Tyrosine Phosphatases; D002118:Calcium