In vivo anti-inflammatory effects of the M20 IL-1 inhibitor: II. Effects on serum reactants.
We have previously described an IL-1 Inhibitor derived from the M20 myelomoncytic cell line. This line also secretes several molecules of IL-1. We have shown that this factor is specific to IL-1 in vitro, as well as in vivo. In vitro IL-1 induced proliferative responses of mouse thymocytes, human T cells and fibroblasts and IL-1 stimulated PGE2 secretion from fibroblasts, were all inhibited by the M20 IL-1 Inhibitor. In vivo, the IL-1 Inhibitor reduced parameters of acute inflammation such as fever, leukocytosis and local inflammation. This study describes additional effects of the M20 IL-1 Inhibitor on inflammatory serum reactants. Levels of corticosterone and fibrinogen were increased by injection of IL-1, and decreased by the IL-1 Inhibitor. IL-1 reduced zinc and iron plasma levels and elevated copper plasma levels. The M20 IL-1 Inhibitor reversed these changes in a dose dependent manner. Similar effects produced by IL-6 and TNF were unaffected by the M20 IL-1 Inhibitor. Our results indicate that the M20 IL-1 Inhibitor acts specifically on IL-1 induced responses in vivo. Therefore we conclude that this IL-1 Inhibitor has a great potential as an anti-inflammatory agent.
Oncology Department, Hadassah University Hospital, Jerusalem, Israel.
V
V
Barak
R
R
Gorodetsky
J
J
Weidenfeld
D
D
Peritt
P
P
Yanai
T
T
Halperin
A J
AJ
Treves
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood / Q000494:pharmacology*; D002412:Cations / Q000097:blood; D003300:Copper / Q000097:blood; D003345:Corticosterone / Q000097:blood; D005340:Fibrinogen / Q000378:metabolism; D007375:Interleukin-1 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D007501:Iron / Q000097:blood; D008670:Metals / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D015032:Zinc / Q000097:blood
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002412:Cations; D007375:Interleukin-1; D008670:Metals; D003300:Copper; D005340:Fibrinogen; D007501:Iron; D015032:Zinc; D003345:Corticosterone