Cellular expression of a GPI-linked T cell activation protein.
A T cell activation protein was identified by generating a monoclonal antibody (anti-D2) against a gamma delta T cell receptor bearing gibbon ape T cell line (MLA144). Immunoprecipitation studies revealed three polypeptides of 180, 150, and 120 kDa. The antigen was also found to be expressed on endothelial cells in vivo and in vitro and on tumor cell lines from a variety of tissues. Studies performed using a variety of antibodies reveal this protein to be identical to an endothelial cell protein previously identified by several antibodies to T cell activation proteins (CDw109). We demonstrate that this protein is anchored in the membrane via a glycosylphosphatidylinositol (GPI) tail in T cells, tumor cells, and endothelial cells. An analysis of tissue sections reveals this protein to be normally highly expressed on vascular endothelial cells.
Laboratory of Infectious Diseases, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
A
A
Haregewoin
K
K
Solomon
R C
RC
Hom
G
G
Soman
J M
JM
Bergelson
A K
AK
Bhan
R W
RW
Finberg
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000302:isolation & purification / Q000378:metabolism*; D001792:Blood Platelets / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D004727:Endothelium / Q000276:immunology / Q000378:metabolism; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D006922:Hylobates; D008213:Lymphocyte Activation / Q000276:immunology*; D008970:Molecular Weight; D011233:Precipitin Tests; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D006023:Glycoproteins; D017261:Glycosylphosphatidylinositols; D016692:Receptors, Antigen, T-Cell, gamma-delta