[Integration and expression of ALV type retroviral vectors in bird embryos: implications for the cartography of cell lineage and in vivo gene transfer].
Retroviruses are, until now, the only efficient method to transfer genes in birds. However, little is known about their integration and expression patterns in the embryo. Embryos were infected with lacZ carrying non-replicative retroviral vectors. Integration and expression patterns appeared strongly correlated in the heart and the skin while they did not coincide for the liver and stomach. We used different viral subgroups. The A subgroup infects both chick and quail while the B and E are efficient only in chick and quail respectively. These results are discussed in relation with different studies aiming to study either cell lineages or transgenesis in birds.
Institut d'Embryologie Cellulaire et Moléculaire du CNRS, Nogent-sur-Marne, France.
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Jaffredo
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Dieterlen-Lièvre
D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D002642:Chick Embryo; D002874:Chromosome Mapping; D015973:Gene Expression Regulation, Leukemic; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D011784:Quail / Q000196:embryology / Q000235:genetics*; D016662:Virus Integration
Intégration et expression de vecteurs rétroviraux de type ALV chez l'embryon d'Oiseau: implications pour la cartographie de lignages cellulaires et le transfert de gènes in vivo.