Differential abilities of the ribosome inactivating proteins luffaculin, luffins and momorcochin to induce abnormalities in developing mouse embryos in vitro.
1. Possible teratogenic activity in the ribosome inactivating proteins luffaculin, luffin-a, luffin-b and momorcochin was tested using cultured mouse embryos. 2. At a dosage of 200 micrograms/ml culture medium, luffin-a and momorcochin did not affect organogenesis in embryos. 3. Luffin-b at a dosage of 100 micrograms/ml brought about decreases in embryo somite number and axial length and induced defects in yolk sac circulation, body axis, optic placodes, branchial apparatus, forelimb buds and cranial neural tube. 4. Luffaculin at a dosage of 100 micrograms/ml caused only reductions in embryo somite number and axial length, but at 200 micrograms/ml produced all of the aforementioned teratogenic effects and defects in otic placodes. 5. The data indicate that ribosome inactivating proteins differ in their potencies as teratogens.
Department of Anatomy, Chinese University of Hong Kong, Shatin, N.T.
W Y
WY
Chan
T B
TB
Ng
H W
HW
Yeung
D000014:Abnormalities, Drug-Induced / Q000473:pathology*; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D006023:Glycoproteins / Q000633:toxicity*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques; D010940:Plant Proteins / Q000633:toxicity*; D011247:Pregnancy; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects*
D006023:Glycoproteins; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C088243:luffaculin protein, Luffa acutangula; C063258:luffin-a protein, Luffa cylindrica; C069038:luffin-b protein, Luffa cylindrica