The effect of high salt intake on Na+, K(+)-ATPase activity of tissues in the rat.
1. The effect of chronic feeding of high salt diet on Na+, K(+)-ATPase activity of heart, liver, skeletal muscle, kidney and aorta was studied in the rat. 2. Groups of rats were either given tap water or 18 g/L saline to drink. After 7 days, 3 months or 12 months, the control group and salt loaded groups were sacrificed and Na+, K(+)-ATPase activity of heart, liver, skeletal muscle and kidney was determined by a coupled enzyme assay and that of the aorta by the K(+)-stimulated hydrolysis of 3-0-methylfluorescein phosphate. 3. Na+, K(+)-ATPase activity of heart, liver, skeletal muscle and aorta were not different between the experimental and control groups at 7 days. After 3 months, Na+, K(+)-ATPase activity of liver in salt-loaded group was higher than the control group. After 12 months of salt loading all tissues examined showed higher Na+, K(+)-ATPase activity compared to control groups. The activity of renal medulla of salt-loaded group was higher than that of control group as early as 7 days. 4. We conclude that long term salt loading causes an increase in the activity of Na+, K(+)-ATPase of kidney, heart, liver, muscle and aorta.
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, NT, Hong Kong.
P W
PW
Li
C S
CS
Ho
R
R
Swaminathan
D000818:Animals; D001011:Aorta / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000652:urine; D012982:Sodium, Dietary / Q000008:administration & dosage / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000378:metabolism
D012982:Sodium, Dietary; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase