Microheterogeneity of alpha 1-acid glycoprotein in early and established rheumatoid arthritis.
OBJECTIVE
To determine whether factors related to disease activity determine changes in the glycosylation of alpha 1-acid glycoprotein (AGP) observed at the early stage of rheumatoid arthritis (RA).


METHODS
Using affinoimmunoelectrophoresis with the lectin concanavalin A (Con-A), the microheterogeneity of serum AGP was studied in patients with early (n = 54) and established (n = 46) RA and the results were expressed as reactivity coefficients (AGP-RC).


RESULTS
When compared with controls (n = 44), AGP-RC values were increased in the patients with very recent RA of no more than 3 months duration (p < 0.05), whereas normal or low AGP-Con-A reactivity was found in the patients with early RA with disease duration exceeding 3 months. In the entire group with early RA, the multiplicative model of regression described the relationship between AGP-Con-A reactivity and disease duration (p < 0.005). AGP variant with low binding affinity to Con-A predominated in the sera of patients with established RA and no relationship between the glycosylation profile of AGP and disease duration was observed in this group. When AGP-RC values were compared in the subgroups of patients with early and established RA with similar disease activity as measured by the Mallya-Mace score or erythrocyte sedimentation rate, there was a significant decrease in AGP-RC with increasing disease activity (p < 0.05).


CONCLUSIONS
In view of our findings, early RA begins as an acute inflammation with increase of AGP-Con-A reactivity and becomes chronic during the first year of the disease. Factors related to disease activity appear important in determining the rate at which RA enters a chronic phase.
Hochrhein Institute for Rheumatism Research and Prevention, Bad Säckingen, Germany.
P
P
Hrycaj
M
M
Sobieska
S
S
Mackiewicz
W
W
Müller
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000378:metabolism / Q000503:physiopathology; D003208:Concanavalin A / Q000378:metabolism; D016207:Cytokines / Q000502:physiology; D005260:Female; D006031:Glycosylation; D006801:Humans; D007122:Immunoelectrophoresis; D008297:Male; D008875:Middle Aged; D009961:Orosomucoid / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D012044:Regression Analysis; D012720:Severity of Illness Index; D013997:Time Factors
D016207:Cytokines; D009961:Orosomucoid; D003208:Concanavalin A