The effect of Tołpa Peat Preparation on the phagocytic and metabolic activity of neutrophils in normothermic rabbits and with pyrogen-induced fever.
The studies on normothermic rabbits show that intravenous administration of Tołpa Peat Preparation (TPP) at a dose of 5 mg/kg for 3 or 6 consecutive days increases the percentage of phagocytizing cells and the number of bacteria phagocytized by a single neutrophil. The stimulated phagocytic activity of neutrophils coincides with increased nitroblue tetrazolium (NBT) reduction. In contrast, a single administration of TPP to rabbits with fever induced by E.coli lipopolysaccharide (LPS) has a modulating effect on the metabolic activity of neutrophils, depending on TPP dose. TPP administered at a dose of 0.5 mg/kg potentiates the stimulating effect of pyrogen on the percent of NBT-positive neutrophils. A tenfold increase of TPP dose (5 mg/kg) reduces the stimulating effect of LPS and a hundredfold increase (50 mg/kg) leads to total inhibition of LPS-induced changes.
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Agricultural University, Wrocław, Poland.
B
B
Obmińska-Domoradzka
M
M
Switała
J
J
Debowy
T
T
Garbuliński
D000596:Amino Acids / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002241:Carbohydrates / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005334:Fever / Q000097:blood / Q000276:immunology*; D006812:Humic Substances / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010084:Oxidation-Reduction; D010587:Phagocytosis / Q000187:drug effects; D011817:Rabbits; D012987:Soil; D014574:Uronic Acids / Q000008:administration & dosage / Q000494:pharmacology*
D000596:Amino Acids; D002241:Carbohydrates; D004338:Drug Combinations; D006812:Humic Substances; D012987:Soil; C084029:Tolpa; D014574:Uronic Acids