Potassium channels of pig articular chondrocytes are blocked by propofol.
The effect of propofol on the voltage-activated potassium channels in pig articular chondrocytes was investigated. Propofol was found to reversibly block the potassium channels in a dose-dependent manner. The blocking effect was voltage-independent and the Hill coefficient was 1.85 +/- 0.18. No changes either in the slope conductance or in the single channel kinetics were observed. The half-blocking concentration (Ec50) was 6.0 +/- 0.49 microM which is much lower than the concentrations used to observe the scavenging effect of the drug in an artificial synovial fluid. Interestingly, Ec50 found in our experiments is also smaller than the blood concentration of propofol used in anaesthesia. These results show that propofol may strongly affect the potassium channels in some non-excitable cells.
Istituto di Fisiologia, Università degli Studi di Trieste, Italy.
J W
JW
Mozrzymas
M
M
Visintin
F
F
Vittur
F
F
Ruzzier
D000818:Animals; D002358:Cartilage, Articular / Q000502:physiology*; D002478:Cells, Cultured; D004553:Electric Conductivity; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000502:physiology*; D015742:Propofol / Q000494:pharmacology*; D013552:Swine
D026902:Potassium Channel Blockers; D015221:Potassium Channels; D015742:Propofol