Serine protease activity in boar seminal vesicles and its immunological similarity to sperm acrosin.
A mouse monoclonal antibody against boar acrosin and antiserum prepared to highly purified acrosin in female rabbits were used to detect the antigen in various fluids and tissues of boars using an indirect immunofluorescence technique. A strong reaction was found in fluid and epithelial tissue of the seminal vesicles as well as in the germinal cells in the testis. No immunoreactivity was detected in tissues of the epididymides and other organs of the boar. The antigens present in seminal vesicle fluid of boars were partially purified by column chromatography. It was demonstrated that two antigens differing in molecular mass were present and both possessed protease and amidase activity. The higher molecular mass antigen eluted from a gel filtration column in a volume identical to that of proacrosin. The same result was obtained in polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS-PAGE). The low molecular mass antigen was eluted from Sephadex G-75 column together with natural protease inhibitors corresponding in molecular mass to less than 20 kDa. The mobility of the antigen in SDS-PAGE was greater than that of chymotrypsin. It is assumed that the protease from seminal vesicle epithelial resembled acrosin in structure and function. Acrosin may therefore not be specific for spermatozoa.
Institute of Molecular Genetics, Czech Academy of Sciences, Prague.
D
D
Cechová
V
V
Jonáková
L
L
Veselský
E
E
Töpfer-Petersen
D000176:Acrosin / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D008297:Male; D012669:Seminal Vesicles / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D013094:Spermatozoa / Q000201:enzymology; D013552:Swine / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D012697:Serine Endopeptidases; D000176:Acrosin