Viral replication and development of specific immunity in macaques after infection with different measles virus strains.
Cynomolgus monkeys (Macaca fascicularis) were experimentally infected with a wild type measles virus (MV) strain (MV-BIL). Following intratracheal inoculation with different infectious doses, the virus could be isolated from peripheral blood mononuclear cells (PBMC), lung lavage cells, and pharyngeal cells. The kinetics of the cell-associated viremia was similar in all infected animals. They developed specific serum IgM, IgG, and neutralizing antibody responses as well as MV-specific T cell-mediated immunity. Monkeys infected intratracheally or intramuscularly with the wild type MV-Edmonston or the attenuated MV-Schwartz strain showed a lower level of PBMC-associated viremia and less pronounced specific IgM responses. Nine months after infection with MV strains, all of the monkeys were protected from intratracheal reinfection with MV-BIL. This monkey model is suitable for study of new generations of vaccines and vaccination strategies for measles.
van Binnendijk
R S
RS
Department of Virology, Erasmus University, Rotterdam, Netherlands.
van der Heijden
R W
RW
van Amerongen
G
G
UytdeHaag
F G
FG
Osterhaus
A D
AD
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D004195:Disease Models, Animal; D006801:Humans; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008168:Lung / Q000166:cytology / Q000382:microbiology; D008252:Macaca fascicularis; D008457:Measles / Q000276:immunology / Q000382:microbiology*; D008459:Measles virus / Q000276:immunology / Q000502:physiology*; D010614:Pharynx / Q000166:cytology / Q000382:microbiology; D013601:T-Lymphocytes / Q000276:immunology; D014766:Viremia / Q000276:immunology / Q000382:microbiology; D014779:Virus Replication
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M