Enzyme inhibition as visualized with the reservoir model: relationships between I50 and inhibition constant(s) of an enzyme inhibitor.
In the reservoir model, enzyme activity is simulated by holes in the wall of a reservoir (metabolic pool) containing liquid (substrate) at a certain level (concentration). The holes are computer drawn to reflect the kinetic properties of the enzyme, namely its maximum velocity (hole area) and Km (hole position). The model is here presented as a tool to intuitively visualize the effect of the different types of reversible enzyme inhibitors on the kinetic properties of an enzyme. The relationships between the concentration of an inhibitor producing 50% inhibition of an enzyme reaction (I50) and its inhibition constant(s) are also discussed.
Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Medicina, Universidad de Extremadura, Badajoz, Spain.
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Ribeiro
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Fontes
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Sillero
D001667:Binding, Competitive; D003198:Computer Simulation; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D004798:Enzymes / Q000187:drug effects* / Q000378:metabolism; D007700:Kinetics; D008433:Mathematics; D008956:Models, Chemical
D004791:Enzyme Inhibitors; D004798:Enzymes