Homogeneity in the distribution of matrix components in the hamster zona pellucida as revealed by backscattered electron imaging fracture-label.
BACKGROUND
Backscattered electron imaging fracture-label (BEI-FL), an adaptation of the fracture-label method for scanning electron microscopy, offers the advantage of providing information about the distribution of antigenic and receptor sites with respect to the three-dimensional organization of tissues and cells over relatively large surfaces. Recently, using post-embedding cytochemistry on thin-sections of Lowicryl-embedded oocytes, a homogenous distribution of glycoproteins in the zona pellucida (ZP) was demonstrated (Kan et al., 1989. Biol. Reprod., 40:585-598, Anat. Rec., 226:37-47; Roux and Kan, 1991. Anat. Rec., 230:347-360). However, it can be argued that the chemical nature of resins and the physical conditions of tissue processing required for post-embedding cytochemistry may introduce changes in the tissue components and result in altered distribution of components. On the other hand, freeze-fracture exposes constituents in a minimally denaturing manner and, since no embedding media are used, binding sites are sterically available to the probe. We have, therefore, applied BEI-FL to examine the distribution of matrix glycoproteins in the ZP of hamster oocytes.


METHODS
Ovaries and cumulus masses obtained from superovulated female golden hamsters were fixed by immersion in 2.5% glutaraldehyde and processed for fracture-label. Tissues were labeled, respectively, with Wheat germ agglutinin (WGA) followed by ovomucoid-colloidal gold, Ricinus communis agglutinin I (RCA I)-colloidal gold or a monoclonal antibody against Hamster Oviductin-1 followed by protein A-gold, and then examined in the scanning electron microscope.


RESULTS
Backscattered electron imaging revealed a homogenous distribution of WGA and RCA I binding sites throughout the cross-fractured matrix of the ZP of ovarian and postovulatory oocytes. Hamster Oviductin-1, an oviductal glycoprotein which is transferred to the ZP of oocytes during oviductal transit, was also found to be uniformly distributed throughout the ZP of postovulatory oocytes.


CONCLUSIONS
Our results indicate that BEI-FL can be advantageously used to examine extracellular matrices and are consistent with the concept that glycoproteins are uniformly distributed throughout the ZP of the hamster oocyte.
Kan
F W
FW
Department of Anatomy, Faculty of Medicine, Université de Montréal, Quebec, Canada.
Zalzal
S
S
Roux
E
E
Nanci
A
A
D000818:Animals; D006224:Cricetinae; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005260:Female; D005614:Freeze Fracturing / Q000379:methods; D006023:Glycoproteins / Q000032:analysis; D006651:Histocytochemistry; D037102:Lectins / Q000032:analysis; D008647:Mesocricetus; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009865:Oocytes / Q000737:chemistry / Q000648:ultrastructure; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins / Q000032:analysis; D015044:Zona Pellucida / Q000737:chemistry* / Q000648:ultrastructure
D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014909:Wheat Germ Agglutinins