The effect of denervation on myosin isoform synthesis in rabbit slow-type and fast-type muscles during terminal differentiation. Denervation induces differentiation into slow-type muscles.
The soleus and gastrocnemius medialis of eight-day-old rabbits were denervated and the effects were examined after fifty-two days by biochemical, cytochemical and mechanical methods. The contralateral soleus exhibited the properties of slow-type muscle, namely a predominance of slow-type myosin isoforms and slow-type oxidative fibers, slow twitch and low maximal velocity for shortening. The contralateral gastrocnemius exhibited the properties of fast-type muscle, namely a predominance of fast-type myosin isoforms and fast-type non-oxidative fibers, fast twitch and high maximal velocity of shortening. Denervation of muscles caused the differentiation of the two muscles towards slow-type muscles. Both denervated soleus and gastrocnemius muscles exhibited a predominance of slow-type myosins (either the normal type, made up of slow heavy and light chains, or the hybrid type, made up of slow heavy and regulatory light chains and fast essential light chains), a predominance of slow-type fibers, and slow mechanical properties. Thus, innervation in rabbit appears to be a determining factor for differentiation into fast-type muscle, but it is not necessary for differentiation into slow-type muscle. This conclusion contradicts the findings of previous studies in rat and thus raises new questions concerning the role of nerves in controlling the expression of myosin isoforms and the differentiation of muscle fibers.
Laboratoire de Biologie Physicochimique, Unité de Recherche Associée au Centre National de la Recherche Scientifique 1131, Université Paris-Sud, Orsay, France.
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A
d'Albis
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Goubel
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R
Couteaux
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C
Janmot
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JC
Mira
D000818:Animals; D001696:Biomechanical Phenomena; D015153:Blotting, Western; D002454:Cell Differentiation; D003714:Denervation; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006651:Histocytochemistry; D066298:In Vitro Techniques; D009132:Muscles / Q000166:cytology / Q000294:innervation / Q000378:metabolism*; D009218:Myosins / Q000096:biosynthesis*; D011817:Rabbits; D051381:Rats
D009218:Myosins