Serologic responses in diagnostic tests for brucellosis in cattle vaccinated with Brucella abortus 19 or RB51.
Serologic responses in the particle concentration fluorescence immunoassay and the card, complement fixation, and tube agglutination tests were measured for 10 weeks after vaccination of cattle with either Brucella abortus 19 or the lipopolysaccharide O-antigen-deficient mutant, strain RB51. The responses of strain 19-vaccinated cattle were positive, whereas those of strain RB51-vaccinated cattle were negative, in all of the tests. These results indicate that cattle vaccinated with strain RB51 fail to produce antibodies that can be detected by conventional serologic tests that are used to diagnose bovine brucellosis.
Brucellosis Research Unit, U.S. Department of Agriculture, Ames, Iowa 50010.
M G
MG
Stevens
S G
SG
Hennager
S C
SC
Olsen
N F
NF
Cheville
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D000942:Antigens, Bacterial; D002003:Brucella abortus / Q000145:classification / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis / Q000276:immunology* / Q000517:prevention & control; D002417:Cattle; D003168:Complement Fixation Tests / Q000662:veterinary; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D012698:Serologic Tests / Q000379:methods; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial