Functional features of oriV of the broad host range plasmid RSF1010 in Pseudomonas aeruginosa.
The broad host range plasmid RSF1010 requires for its replication in Escherichia coli three plasmid-encoded proteins and specific nucleotide sequences ssiA, ssiB, and iterons in the oriVRSF1010. In Pseudomonas aeruginosa, a recombinant mini-RSF1010 plasmid lacking ssiB lost its replication ability, but a miniplasmid lacking ssiA or carrying a primosome assembly site in place of ssiA could replicate. Moreover, ssiA, as a sole ssi signal, in the orientation that ssiB had originally taken was sufficient for replication of the miniplasmid. These results indicated that only one RSF1010-specific ssi signal in the orientation that ssiB takes in wild-type oriVRSF1010 was essential for replication of RSF1010. Replication of the miniplasmids was dependent on the three plasmid-encoded proteins, RepA, B', and C, as in E. coli.
Higashi
A
A
Research and Development Center, DAIKEN Corporation, Okayama, Japan.
Sakai
H
H
Honda
Y
Y
Tanaka
K
K
Miao
D M
DM
Nakamura
T
T
Taguchi
Y
Y
Komano
T
T
Bagdasarian
M
M
D010584:Bacteriophage phi X 174 / Q000235:genetics; D004269:DNA, Bacterial; D004279:DNA, Viral; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012093:Replicon
D004269:DNA, Bacterial; D004279:DNA, Viral