Influence of tumor site on the therapy of murine kidney cancer.
OBJECTIVE
The purpose of this study was to evaluate the influence of tumor site on the therapy of an experimental murine kidney cancer model, Renca.


METHODS
Equal number of tumor cells were injected subcutaneously, intraperitoneally, sub-renal capsule, and intravenously to induce tumors. The animals were then assessed for growth of the primary tumor, metastases and survival. Immunohistochemistry and flow cytometry was performed to identify the phenotype of infiltrating lymphocytes. Tumor bearing animals were treated with high dose interleukin-2 or whole body radiation, and response of the primary tumors as well as the metastases was assessed.


RESULTS
Renca tumors grew well regardless of the methods of induction. Spontaneous metastasis could be best induced in the sub-renal capsule model. More consistent numbers of pulmonary metastases were obtained by intravenous injection of the tumor cells. Immunotherapy was able to reduce the size of the primary tumor as well as the number of metastasis. Whole body radiation caused some reduction in the primary tumor but did not have a major effect in reducing metastasis.


CONCLUSIONS
The Renca model is a suitable animal model to study the response to different therapeutic interventions. The site of the tumor growth is not a significant variable in the response to treatment.
Department of Urology, Wayne State University School of Medicine, Detroit, MI 48201.
A
A
Chakrabarty
G G
GG
Hillman
R L
RL
Maughan
D W
DW
Visscher
E
E
Ali
J E
JE
Pontes
G P
GP
Haas
D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy / Q000628:therapy*; D000818:Animals; D002455:Cell Division; D005260:Female; D006801:Humans; D007167:Immunotherapy; D007376:Interleukin-2 / Q000627:therapeutic use*; D007680:Kidney Neoplasms / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy / Q000628:therapy*; D007700:Kinetics; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000627:therapeutic use; D012016:Reference Values; D013154:Spleen / Q000276:immunology; D013370:Subrenal Capsule Assay; D016176:T-Lymphocyte Subsets / Q000276:immunology; D016042:Transplantation, Heterotopic
D007376:Interleukin-2; D011994:Recombinant Proteins