Determination of codeine and its metabolites in microsomal incubates by high-performance liquid chromatography.
A rapid and sensitive HPLC method has been developed for the determination of codeine, norcodeine and morphine in small volumes of a biological matrix, using a cyanopropyl column and a combination of coulometric and UV detection. The compounds were isolated using C18 solid-phase extraction cartridges prior to quantitative analysis. The limit of detection was 250 pg/ml for morphine and 5 ng/ml for both norcodeine and codeine. Recovery of each compound was greater than 90% and intra- and inter-assay precision was better than 10%. The method has been used to study the metabolism of codeine in microsomal incubations.
Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
M
M
Pawula
P N
PN
Shaw
D A
DA
Barrett
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003061:Codeine / Q000031:analogs & derivatives / Q000032:analysis* / Q000378:metabolism; D004563:Electrochemistry; D008862:Microsomes, Liver / Q000737:chemistry* / Q000378:metabolism; D009020:Morphine / Q000032:analysis; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
C010414:norcodeine; D009020:Morphine; D003061:Codeine