Detection of rat, porcine, and bovine group B rotavirus in fecal specimens by solid-phase enzyme immunoassay.
An enzyme immunoassay that uses easily regenerated reagents was developed and evaluated for the ability to detect group B rotaviruses (GBR) in fecal specimens. Homologous rat GBR and heterologous porcine and bovine GBR were detected by this immunoassay, although a human GBR isolate was not. This immunoassay should prove useful in studies of GBR infections of animals.
Vonderfecht
S L
SL
Upjohn Company, Kalamazoo, Michigan 49001.
Lindsay
D A
DA
Eiden
J J
JJ
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D003967:Diarrhea / Q000382:microbiology; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D007202:Indicators and Reagents; D051381:Rats; D012401:Rotavirus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000382:microbiology; D012680:Sensitivity and Specificity; D013552:Swine
D000956:Antigens, Viral; D007202:Indicators and Reagents