Establishment of two distinct anti-cardiolipin antibody-producing cell lines from the same individual by Epstein-Barr virus transformation.
Two anti-cardiolipin antibody (ACA)-producing cell lines were established, using Epstein-Barr virus transformation followed by a repeated cluster-picking from the same individual with antiphospholipid syndrome who had a history of 8 consecutive fetal losses and deep venous thrombosis. Characterization of the two ACAs derived from these cell lines revealed that one (Ab-019, subclass IgM, kappa) reacted exclusively with cardiolipin and showed strong lupus anticoagulant activity, while the other (Ab-226, subclass IgM, lambda) reacted with negatively charged phospholipids such as phosphatidylserine and phosphatidylglycerol, as well as cardiolipin. Furthermore, Ab-226 showed reactivity with human umbilical vein endothelial cells whereas Ab-019 did not. It is suggested that ACA is heterogeneous even in the same individual, and that reactivity against negatively charged phospholipids corresponds to reactivity against endothelial cell.
Department of Obstetrics and Gynecology, Niigata University School of Medicine, Japan.
I
I
Hasegawa
T
T
Suzuki
S
S
Ishii
K
K
Takakuwa
K
K
Tanaka
D000328:Adult; D017153:Antibodies, Anticardiolipin / Q000096:biosynthesis*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000276:immunology*; D005260:Female; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans
D017153:Antibodies, Anticardiolipin