Physical map of Mycoplasma gallisepticum.
Physical chromosomal maps of two Mycoplasma gallisepticum strains, R and ATCC 19610, were constructed by using field inversion gel electrophoresis. To assist in the ordering of chromosomal fragments and the construction of the chromosomal maps, the gram-positive transposon Tn4001 was modified to serve as a mobile restriction site. The total sizes of the M. gallisepticum R and ATCC 19610 genomes were estimated to be 1,037 and 998 kb, respectively. The restriction enzyme locations for EagI and SmaI were determined along with several transposon insertion sites. The two strain maps were similar except for three small deletions and one additional EagI site in strain ATCC 19610.
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Veterinary Medical Research Institute, Iowa State University, Ames.
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D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000379:methods; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C046751:endodeoxyribonuclease XmaIII; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific