Digoxigenin-tagged anti-GFAP and multiple labelling of human glia, vessels and beta-amyloid.
Cytochemical procedures for the combined demonstration of astroglia, microglia, blood vessels and beta-amyloid in the human brain were developed. These multiple label experiments include the first adaptation of the digoxigenin-antidigoxigenin technique to immunocytochemistry by using digoxigenylated antibodies directed against the glial fibrillary acidic protein and its visualization in astrocytes with an antidigoxigenin-peroxidase conjugate and diaminobenzidine as chromogen. Furthermore, the specific labelling of microglial cells was performed with the novel enhanced polymer one-step staining technique, non-interfering with conventional detection systems. The demonstration of spatial relationships between glial and vascular components in normal tissue and their alterations, e.g. in Alzheimer's disease, might provide insights into the time course and localization of pathological events.
Department of Neurochemistry, Paul Flechsig Institute for Brain Research, University of Leipzig, Germany.
W
W
Härtig
D
D
Hausen
K
K
Brauer
T
T
Arendt
V
V
Bigl
G
G
Brückner
D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001253:Astrocytes / Q000737:chemistry* / Q000648:ultrastructure; D015415:Biomarkers; D001921:Brain / Q000098:blood supply / Q000473:pathology*; D001923:Brain Chemistry; D004076:Digoxigenin; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D017628:Microglia / Q000737:chemistry* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000032:analysis*; D013702:Temporal Lobe / Q000737:chemistry / Q000648:ultrastructure
D016229:Amyloid beta-Peptides; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D004076:Digoxigenin