Astragalus membranaceus and Polygonum multiflorum protect rat heart mitochondria against lipid peroxidation.
We isolated rat heart mitochondria and induced lipid peroxidation with ADP and FeSO4. Oxygen consumption and MDA formation were measured for quantitating the amount of lipid peroxidation. Using these methods, we screened the water extracts of 14 Chinese medicinal herbs for their effect on lipid peroxidation. It was found that Astragalus membranaceus inhibited 42.1 +/- 3.4% of oxygen consumption and 39.8 +/- 3.2% of MDA production at concentration of 2 mg dried herb/ml mitochondrial suspension. At the same concentration, Polygonum multiflorum inhibited 52.1 +/- 7.3% of oxygen consumption and 50.9 +/- 5.3% of MDA production. Other herbs did not inhibit lipid peroxidation to 50% of control at concentration up to 6 mg dried herb/ml mitochondrial suspension. Purification and identification of the active component(s) in Astragalus membranaceus and Polygonum multiflorum as well as their clinical application await further studies.
Hong
C Y
CY
Institute of Traditional Medicine, Yang-Ming Medical College, Taipei, Taiwan.
Lo
Y C
YC
Tan
F C
FC
Wei
Y H
YH
Chen
C F
CF
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D027885:Astragalus propinquus; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005290:Ferric Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D005290:Ferric Compounds; D010936:Plant Extracts; C024823:ferric sulfate; D008315:Malondialdehyde; D000244:Adenosine Diphosphate