Characterization of the determinant (traB) encoding sex pheromone shutdown by the hemolysin/bacteriocin plasmid pAD1 in Enterococcus faecalis.
pAD1 is a hemolysin/bacteriocin plasmid originally identified in Enterococcus faecalis DS16. It encodes a mating response to a peptide sex pheromone, cAD1, secreted by recipient bacteria. Once pAD1 is acquired, production of the pheromone ceases--a trait related in part to a determinant designated traB. Here we report the nucleotide sequence of traB and the position of several transposon insertions resulting in the characteristic self-induction phenotype. The deduced product has a mass of 43.7 kDa with the C-terminal third consisting primarily of hydrophobic amino acid residues.
Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor 48109.
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D003086:Bacteriocin Plasmids; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D013293:Enterococcus faecalis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006459:Hemolysin Factors; D008969:Molecular Sequence Data; D012724:Sex Attractants / Q000235:genetics*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012724:Sex Attractants; C082576:traB protein, Bacteria