Protective effects of idebenone on vascular endothelial cells against toxicity induced by oxidatively modified low density lipoprotein.
We examined the protective effects of idebenone, 6-(10-hydroxydecyl)-2,3-dimethoxy-5-methyl-1,4-benzoquinone, on the cytotoxicity of oxidatively-modified low density lipoprotein (oxLDL), using cultured vascular endothelial cells from fetal bovine aorta. When the cells were incubated with idebenone, the toxicity of oxLDL was inhibited dose-dependently (10(-7)-10(-5) M). When cells were preincubated with idebenone, the toxicity of oxLDL was inhibited only at a high concentration (10(-5) M). However, idebenone had no significant effects on copper-induced modification of LDL. The protective effects of idebenone on oxLDL-induced endothelial toxicity may be beneficial for the inhibition of the development of atherosclerosis in the brain and other arterial systems.
Naito
M
M
Department of Geriatrics, Nagoya University School of Medicine, Japan.
Hayashi
T
T
Yamada
K
K
Asai
K
K
Yoshimine
N
N
Iguchi
A
A
D000818:Animals; D001013:Aorta, Thoracic; D016227:Benzoquinones / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood / Q000187:drug effects / Q000302:isolation & purification; D008315:Malondialdehyde / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014451:Ubiquinone / Q000031:analogs & derivatives; D014810:Vitamin E / Q000494:pharmacology
D016227:Benzoquinones; D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; D014451:Ubiquinone; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D008315:Malondialdehyde; D003300:Copper; D007770:L-Lactate Dehydrogenase; C036619:idebenone