Differential evolution of substrates for an RNA enzyme in the presence and absence of its protein cofactor.
Selection of substrates for an RNA enzyme, the catalytic subunit of RNAase P from E. coli, has been carried out by simulation of evolution in vitro in the presence and absence of the protein cofactor of the enzyme. In the presence of the protein, substrates resembling precursor tRNAs, which were readily cleaved by the catalytic RNA, were selected in addition to others, with different sequences and structures (one of which resembled the precursor to 4.5S RNA) that were not readily cleaved by the catalytic RNA alone. The ribonucleoprotein enzyme is more versatile than the RNA enzyme, and our results suggest that it and 4.5S RNA may have evolved after ancestral tRNAs.
Liu
F
F
Department of Biology, Yale University, New Haven, Connecticut 06520.
Altman
S
S
D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D005075:Biological Evolution; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000378:metabolism; D043262:Ribonuclease P; D013379:Substrate Specificity
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D016337:RNA, Catalytic; D012330:RNA, Double-Stranded; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli