Tumorigenicity of BALB3T3 A31 cells transfected with hamster-complement-C1s cDNA.
Hamster-complement-C1s cDNA was inserted into an expression plasmid BCMGSNeo (BCMGSNeoHACS). BALB/c mouse fibroblast A31 cells, which do not produce C1s, were transfected with BCMGSNeoHACS and the transfectants were selected with G418. Normal C1s production by the transfectants was confirmed by Northern and immunoblot analysis and by an esterase assay. To examine the tumorigenicity of the transfectants, 1 x 10(6) cells were injected s.c. into 6-week-old BALB/c nu/nu mice. Three C1s cDNA transfectants (A3CS9, A3CS12, A3CS13) formed tumors whereas both A31 and A31 transfected with the vector alone (A3BCM1 and A3BCM3) did not. The tumors derived from the transfectants showed invasive growth, and many capillaries were observed in the tumors. A tumor derived from A3CS13 was examined immunohistochemically and found to be reactive with an anti-C1s monoclonal antibody. Tumor cells were cultured in vitro again and C1s secreted into the culture medium was examined by immunoblot analysis. C1s synthesized by the tumor cells derived from A3CS13 maintained its biological functions. Tumor cells derived from A3CS9 and A3CS12 cells, however, produced C1s having abnormal disulfide bonds.
Sakai
N
N
Division of Physiology and Pathology, National Institute of Radiological Science, Chiba, Japan.
Kusunoki
M
M
Nishida
M
M
Toyoguchi
T
T
Fukutomi
H
H
Sakiyama
H
H
D016475:3T3 Cells / Q000502:physiology; D000818:Animals; D002455:Cell Division / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D003173:Complement C1s / Q000235:genetics*; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D010957:Plasmids / Q000235:genetics; D014162:Transfection
D018076:DNA, Complementary; D003173:Complement C1s