Mathematical modelling for the generation of L-[3-2H,3-13C]lactic acid isotopomers by erythrocytes exposed to either D-[1-13C]glucose or D-[6-13C]glucose in the presence of 2H2O.
The production of C3-trisdeuterated, bisdeuterated, monodeuterated or non-deuterated L-[3-13C]lactate by human erythrocytes exposed to either D-[1-13C]glucose or D-[6-13C]glucose in the presence of 2H2O can be assessed by 13C NMR spectroscopy. Such a deuteration may occur at the level of the reactions catalyzed by phosphoglucoisomerase, phosphomannoisomerase, pyruvate kinase and glutamate-pyruvate transaminase. In this report, a mathematical model is proposed for the analysis of experimental data. It allows to estimate the relative extent of deuteration at each step of D-glucose metabolism. This approach may thus provide novel information on the extent of back-and-forth interconversion of either hexose 6-phosphates in both the phosphoglucoisomerase and phosphomannoisomerase reactions or pyruvate and L-alanine in the reaction catalyzed by glutamate-pyruvate transaminase.
Laboratory of Experimental Medicine, Erasmus Medical School, Brussels Free University (ULB), Belgium.
W J
WJ
Malaisse
M
M
Biesemans
R
R
Willem
D000410:Alanine Transaminase; D002247:Carbon Isotopes; D017666:Deuterium Oxide / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D005636:Fructosephosphates / Q000737:chemistry; D005947:Glucose / Q000494:pharmacology*; D005956:Glucose-6-Phosphate Isomerase; D006801:Humans; D007773:Lactates / Q000097:blood* / Q000737:chemistry; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D008359:Mannose-6-Phosphate Isomerase; D008433:Mathematics; D008954:Models, Biological; D011770:Pyruvate Kinase / Q000737:chemistry; D014316:Tritium
D002247:Carbon Isotopes; D005636:Fructosephosphates; D007773:Lactates; D014316:Tritium; D019344:Lactic Acid; C027618:fructose-6-phosphate; D000410:Alanine Transaminase; D011770:Pyruvate Kinase; D008359:Mannose-6-Phosphate Isomerase; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose; D017666:Deuterium Oxide