Degradability of chlorine-free bleachery effluent lignins by two fungi: effects on lignin subunit type and on polymer molecular weight.
A bleachery effluent from a sulfite process pulp mill, which was extracted with alkali and treated with oxygen and hydrogen peroxide (EOP), was treated with two fungi, Trametes versicolor and Stagonospora gigaspora. Trametes versicolor did not cause any depolymerization or degradation of effluent lignins but increased the amount of chromophores, whereas S. gigaspora depolymerized the EOP lignins and caused a substantial reduction in aromatic compounds. For both fungal treatments, CuO oxidation caused a decrease in the yield of the aldehydes within the vanillyl and p-hydroxy phenol families, which was faster than the rates of decrease in the yields of the corresponding acids and ketones. However, only S. gigaspora caused changes in the pattern of the 11 characteristic lignin phenols produced by CuO oxidation, reflecting a preferential metabolism of some phenolic precursors. This fungus decreased the yield of total vanillyl phenols (V), which contributed the bulk of the 11 lignin oxidation products, from 93% initially to 59%. As a consequence, coumaryl (C), syringyl (S), and p-hydroxy phenols (P) became relatively enriched to 1.2, 6.5, and 33%, respectively. The stability of EOP-lignin constituent subunits is S > P > C > V. The two fungi differed significantly in their level of enzyme activities. In effluent-free medium, the ratio of laccase to peroxidase was higher for T. versicolor than for S. gigaspora. The presence of EOP-lignins significantly increased this ratio. No lignin peroxidase was detected but manganese peroxidase and laccase were detected during degradation activities.
Bergbauer
M
M
Fachgebiet Botanik und Mikrobiologische Chemie, Technische Universität, Berlin, Germany.
Eggert
C
C
D001487:Basidiomycota / Q000201:enzymology / Q000378:metabolism; D001673:Biodegradation, Environmental; D005658:Fungi / Q000201:enzymology / Q000378:metabolism*; D007220:Industrial Waste; D042845:Laccase; D008031:Lignin / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D010209:Paper; D010636:Phenols / Q000378:metabolism; D013045:Species Specificity
D007220:Industrial Waste; D010636:Phenols; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase