Identification of the rpmF-plsX-fabH genes of Rhodobacter capsulatus.
The rpmF-plsX-fabH gene cluster of Rhodobacter capsulatus homologous to that of Escherichia coli was identified. rpmF encodes ribosomal protein L32, plsX plays an undefined role in membrane lipid synthesis, and fabH encodes beta-ketoacyl-acyl carrier protein synthase III. The R. capsulatus plsX gene complemented a defect in an E. coli strain with the plsX50 mutation. Overproduction of the fabH gene product of R. capsulatus in E. coli resulted in dramatically increased beta-ketoacyl-acyl carrier protein synthase III activity. These results indicate that plsX and fabH apparently function the same in R. capsulatus as in E. coli.
Department of Biochemistry and Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061-0308.
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Carty
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Colbeau
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Vignais
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Larson
D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Q000096:biosynthesis / Q000235:genetics*; D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D016354:Rhodobacter capsulatus / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D000085:Acetates; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D010743:Phospholipids; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C490246:plsX protein, E coli; C088150:plsX protein, bacteria; C041806:ribosomal protein L32; C077402:3-ketoacyl-acyl carrier protein synthase III; D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; D019342:Acetic Acid