Free radical activation of acetaldehyde and its role in protein alkylation.
The formation of carbon centered free radicals, identified as methylcarbonyl species, was observed using ESR spectroscopy and the spin trapping agent 4-pyridyl-1-oxide-N-t-butyl nitrone (4-POBN) during the oxidation of acetaldehyde by xanthine oxidase. The reaction was dependent upon the presence of OH. radicals and was inhibited by the addition of superoxide dismutase, catalase or OH. radical scavengers. The generation of methylcarbonyl radicals was associated with a doubling of stable acetaldehyde adducts with serum albumin, and 4-POBN or superoxide dismutase and catalase, completely blocked this effect. Thus, methylcarbonyl radicals contributed to acetaldehyde-mediated protein alkylation which is involved in causing toxic as well as immunological reactions ascribed to acetaldehyde.
Department of Medical Sciences, University of Turin, Novara, Italy.
E
E
Albano
P
P
Clot
A
A
Comoglio
M U
MU
Dianzani
A
A
Tomasi
D000079:Acetaldehyde / Q000378:metabolism*; D000478:Alkylation; D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000378:metabolism; D010084:Oxidation-Reduction; D011506:Proteins / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D005609:Free Radicals; D011506:Proteins; D014969:Xanthine Oxidase; D000079:Acetaldehyde