Effect of surfactant mixtures on irritant contact dermatitis potential in man: sodium lauroyl glutamate and sodium lauryl sulphate.
Our purpose was to ascertain the irritant contact dermatitis potential of sodium lauroyl glutamate (SLG) and to assess its possible anti-irritating potential in a surfactant mixture on human skin, using visual scores and measurement of transepidermal water loss (TEWL). 15 healthy adult volunteers free of skin disease and with no history of atopic dermatitis were treated with sodium lauryl sulphate (SLS) solution (1%, 0.75%, 0.50%, 0.25%), 1% SLG solution, and 1% surfactant mixture solutions, M1 (75/25), M2 (50/50), M3 (25/50) (SLG/SLS). We applied 100 microliters of solution using a polypropylene chamber for 24 h. Application sites for each solution were randomized to minimize anatomical bias. We measured baseline TEWL before test solution application. After removal of the patches, each site was visually graded and TEWL recorded with an evaporimeter daily for 4 days. The visual scores and TEWL values of 1% SLG solutions were lower than those of the other test solutions, except the vehicle control (deionized water). 1% surfactant mixture solutions showed lower visual scores and TEWL values than 1% SLS solution. Increase of SLG concentration decreased the visual scores and TEWL values. Comparing the visual scores and TEWL values of M1 and 0.25% SLS solution, M2 and 0.50% SLS solution, and M3 and 0.75% SLS solution, M1, M2, and M3 showed lower values, although not statistically different. These findings suggest that SLG is a mild surfactant and its utilization can decrease irritation potential in SLS and possibly other mixtures. This model may offer a facile system for screening the surfactant and other mixtures for decreased irritancy potential.
Department of Dermatology, School of Medicine, University of California San Francisco.
C H
CH
Lee
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Kawasaki
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Maibach
D000328:Adult; D003877:Dermatitis, Contact / Q000209:etiology*; D017453:Dermatitis, Irritant / Q000209:etiology; D005260:Female; D005971:Glutamates / Q000009:adverse effects* / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D013501:Surface-Active Agents / Q000009:adverse effects* / Q000494:pharmacology; D014870:Water Loss, Insensible / Q000187:drug effects
D005971:Glutamates; D013501:Surface-Active Agents; C085318:N-dodecanoylglutamic acid