Liquid chromatographic determination of carnitine by precolumn derivatization with pyrene-1-carbonyl cyanide.
A method is described for the determination of carnitine chloride in pharmaceutical preparations by precolumn derivatization and high-performance liquid chromatography (HPLC). Carnitine chloride is derivatized by reaction with pyrene-1-carbonyl cyanide (PCC) in dimethyl sulphoxide. The PCC derivative of carnitine is then used for quantitative HPLC analysis on a cation-exchange column with fluorescence detection. The calibration graph was linear over a sample concentration range from 2.0 to 20 micrograms/ml; the limit of detection was 0.5 microgram/ml under the conditions studied. The proposed method was applied satisfactorily to the determination of carnitine chloride in pharmaceutical preparations.
Kamata
K
K
Tokyo Metropolitan Research Laboratory of Public Health, Japan.
Takahashi
M
M
Terasima
K
K
Nishijima
M
M
D002331:Carnitine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009570:Nitriles; D011721:Pyrenes; D012015:Reference Standards; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009570:Nitriles; D011721:Pyrenes; C088296:pyrene-1-carbonylcyanide; D002331:Carnitine