Disruption of endothelial barrier function: relationship to fluidity of membrane extracellular lamella.
1. Endothelial cells were cultured in tissue culture flasks or on microcarrier beads and labeled with a lipid specific spin-label. 2. Exposure of endothelial cells to benzyl alcohol caused a dose- and time-dependent increase in membrane fluidity using electron spin resonance (ESR). Maximum fluidity was reached after a 5-min exposure to 100 mM benzyl alcohol. 3. Albumin permeability across endothelial cells cultured on micropore filters was used as an indication of endothelial monolayer integrity. 4. A significant increase in permeability occurred with 50 mM benzyl alcohol. Maximal albumin permeability was reached after a 5-min exposure to 100 mM benzyl alcohol.
Department of Nutrition and Food Science, University of Kentucky, Lexington 40506-0054.
A
A
Alvarado
D A
DA
Butterfield
B
B
Hennig
D000418:Albumins / Q000493:pharmacokinetics; D000818:Animals; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D004578:Electron Spin Resonance Spectroscopy; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D008560:Membrane Fluidity / Q000187:drug effects; D013113:Spin Labels; D013552:Swine
D000418:Albumins; D001592:Benzyl Alcohols; D013113:Spin Labels; D019905:Benzyl Alcohol