GC-MS determination of phenobarbitone entrapped in poly-epsilon-caprolactone nanocapsules.
In order to evaluate the concentration of a hydrophilic drug, phenobarbitone, in a suspension of poly-epsilon-caprolactone nanocapsules, a gas chromatographic-mass spectrometric procedure, performed after methylation of the drug, was developed and validated. Free phenobarbitone (in solution in the liquid phase), released phenobarbitone (after opening the nanocapsules with ethyl acetate) and total entrapped phenobarbitone (after extraction with methylene chloride), were measured. Experimental results for four lots with various concentrations showed that the highest preparation of entrapped drug (80%) was obtained for a total concentration of 3.64 mg ml-1 in the nanocapsule suspension.
Laboratoire de Pharmacochimie-Biopharmacie, Faculté de Médecine et de Pharmacie de Rouen, Saint-Etienne du Rouvray, France.
M
M
Berrabah
D
D
André
F
F
Prévot
A M
AM
Orecchioni
O
O
Lafont
D004339:Drug Compounding; D008401:Gas Chromatography-Mass Spectrometry; D008752:Methylene Chloride / Q000737:chemistry; D010634:Phenobarbital / Q000032:analysis*; D011091:Polyesters / Q000737:chemistry*
D011091:Polyesters; C016240:polycaprolactone; D008752:Methylene Chloride; D010634:Phenobarbital