Mitochondrial DNA restriction map for the Mediterranean fruit fly, Ceratitis capitata.
Molecular genetic research on the Mediterranean fruit fly, Ceratitis capitata, will provide tools to permit determination of source populations for new pest infestations. Restriction fragment length polymorphism (RFLP) of mitochondrial DNA provides some interpopulation discrimination. A restriction map, including the informative variable EcoRV and XbaI restriction sites, is constructed for the Mediterranean fruit fly, and several restriction sites are associated with specific gene regions based on polymerase chain reaction-RFLP and sequence analyses. A partial sequence of the mitochondrial 16S ribosomal RNA gene is reported.
McPheron
B A
BA
Department of Entomology, Penn State University, University Park, Pennsylvania 16802-3508.
Gasparich
G E
GE
Han
H Y
HY
Steck
G J
GJ
Sheppard
W S
WS
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D004175:Diptera / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D006239:Haplotypes; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S