Isolation of glucosinolate degrading microorganisms and their potential for reducing the glucosinolate content of rapemeal.
A range of microorganisms were assessed for their ability to degrade glucosinolates, using sinigrin (2-propenyl glucosinolate) as a model compound. Eight different species capable of growing on sinigrin as a sole carbon source were isolated. These were predominantly Gram-positive bacteria which also degraded the natural glucosinolates within rapemeal. Growth of the majority of these organisms in a sinigrin/glucose liquid medium wass biphasic; glucose was utilised during the initial rapid phase of growth. The ability to degrade sinigrin was found to be unstable and was rapidly and irreversibly lost when organisms were cultured on sinigrin-free media.
Brabban
A D
AD
Department of Genetics and Microbiology, University of Liverpool, UK.
Edwards
C
C
D000821:Animal Feed; D001419:Bacteria / Q000378:metabolism*; D001937:Brassica / Q000737:chemistry*; D005961:Glucosinolates / Q000032:analysis / Q000378:metabolism*
D005961:Glucosinolates; C010330:sinigrin