Effects of cholinergic stimulation and aldosterone administration on salivary parotid secretion in the brushtail possum, Trichosurus vulpecula.
Parotid salivation was stimulated by infusion of bethanechol chloride into anaesthetized brushtail possums to ascertain maximal flow rates, salivary composition and dietary adaptations of salivary function. Secretion rates for one gland ranged from 5.3 +/- 0.16 to 84.3 +/- 3.20 microliters/min (2.4 +/- 0.07 to 37.8 +/- 1.43 microliters/min per kg body weight). Salivary osmolality (160.8 +/- 15.39 to 248.2 +/- 8.70 mosmol/kg) and the concentrations of Na (63.1 +/- 10.93 to 124.1 +/- 5.52 mmol/l) and HCO3 (19.5 +/- 3.41 to 89.0 +/- 3.19 mmol/l) were positively correlated with flow rate. The concentrations of urea (7.8 +/- 0.64 to 4.6 +/- 0.33 mmol/l), PO4 (2.6 +/- 0.25 to 0.96 +/- 0.10 mmol/l), H+ (46.2 +/- 16.30 to 9.0 +/- 1.51 nequiv/l), K (21.4 +/- 3.73 to 13.7 +/- 2.33 mmol/l), Ca (3.7 +/- 0.53 to 2.2 +/- 0.27 mmol/l) and Mg (0.3 +/- 0.07 to 0.04 +/- 0.007 mmol/l) fell with increasing flow rate. The relations between flow rate and amylase activity (22.6 +/- 10.47 to 10.5 +/- 3.68 mu kat/l), protein concentration (2.7 +/- 0.61 to 1.3 +/- 0.28 g/l), and Cl concentration (62.1 +/- 6.88 to 50.6 +/- 6.37 mmol/l) were inconsistent between experiments. Salivary Na/K ratios were not decreased by infusion of aldosterone (2.2-22.2 nmol/h for 5 h), showing that the gland of Na-replete possums is unresponsive to short-term increases in mineralocorticoids. The low salivary amylase activity, less than that of kangaroos, presumably reflects the interaction of evolutionary history of the possum with its natural low-starch diet.(ABSTRACT TRUNCATED AT 250 WORDS)
Scott
N A
NA
School of Biological Science, University of N.S.W., Kensington, Australia.
Beal
A M
AM
D000109:Acetylcholine / Q000494:pharmacology; D000450:Aldosterone / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D001626:Bethanechol Compounds / Q000494:pharmacology; D004573:Electrolytes / Q000378:metabolism; D005260:Female; D008297:Male; D009893:Opossums; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism*; D010949:Plasma / Q000737:chemistry* / Q000201:enzymology; D012044:Regression Analysis; D012463:Saliva / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000032:analysis; D012636:Secretory Rate / Q000187:drug effects*
D001626:Bethanechol Compounds; D004573:Electrolytes; D012471:Salivary Proteins and Peptides; D000450:Aldosterone; D000109:Acetylcholine