Effect of protein adsorption on the surface charge characteristics of aluminium-containing adjuvants.
The effect of adsorbing two model proteins, bovine serum albumin and lysozyme, on the point of zero charge of aluminium-containing vaccine adjuvants was studied. At physiological pH, the adsorption of the negatively charged albumin (isoelectric point = 5.0) by aluminium hydroxide adjuvant (point of zero charge = 11.1) resulted in a decrease in the point of zero charge. In contrast, the adsorption of positively charged lysozyme (isoelectric point = 9.6) by the negatively charged aluminium phosphate adjuvant (point of zero charge = 5.0) resulted in an increase in the point of zero charge. The surface charge characteristics of the aluminium-containing adjuvant dominated at low protein coverage. In contrast, the surface charge characteristics of the adsorbed protein dominated at high protein coverage. Therefore, the physicochemical properties of the antigen-adjuvant complex and not the adjuvant alone should be considered during vaccine preparation.
Department of Industrial and Physical Pharmacy, Purdue University, West Lafayette, IN 47907.
R
R
al-Shakhshir
F
F
Regnier
J L
JL
White
S L
SL
Hem
D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000327:Adsorption; D017607:Aluminum Compounds / Q000737:chemistry*; D000536:Aluminum Hydroxide / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D007526:Isoelectric Point; D009113:Muramidase / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D010710:Phosphates; D012710:Serum Albumin, Bovine; D000536:Aluminum Hydroxide; D009113:Muramidase; C012714:aluminum phosphate