The effect of media composition on EDTA degradation by Agrobacterium sp.
EDTA degradation by an Agrobacterium sp. has been examined by quantifying 14C-labeled CO2 produced from iron-[2-14C] EDTA and by measured loss of nonlabeled EDTA by HPLC. Fe-EDTA degradation resulted in a rise in pH, nitrate concentration, and ammonia concentration. Addition of glycerol resulted in suppression of Fe-EDTA degradation and in a decrease in pH and NH4+ concentration in the media. Addition of peptone or yeast extract did not affect degradation. Some of the components (e.g., biotin) of the media are not necessary for growth and biodegradation. Although cobalt-EDTA cannot be degraded, ferrous iron can be added to displace the cobalt.
Environmental Sciences Division, Oak Ridge National Laboratory, TN 37831-6036.
A V
AV
Palumbo
S Y
SY
Lee
P
P
Boerman
D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D003037:Cobalt Radioisotopes / Q000378:metabolism; D003470:Culture Media; D004492:Edetic Acid / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D005990:Glycerol / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology; D012231:Rhizobium / Q000378:metabolism*; D014876:Water Pollution
D003037:Cobalt Radioisotopes; D003470:Culture Media; D005290:Ferric Compounds; D005296:Ferrous Compounds; D004492:Edetic Acid; D009584:Nitrogen; D005990:Glycerol