Composition of the transfer medium is crucial for high-resolution immunocytochemistry of cryosectioned human neutrophils.
One of the more routine steps associated with cryosectioning of ultra-thin and semi-thin sections for immunocytochemistry is the transfer of sections to EM grids or coverslips. In a study of the granules of human neutrophils, we have found that the composition of the transfer medium was crucial to the success of these experiments.
Takizawa
T
T
Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, Columbus 43210.
Robinson
J M
JM
D003470:Culture Media; D005629:Frozen Sections / Q000379:methods; D005780:Gelatin; D006801:Humans; D007150:Immunohistochemistry; D009504:Neutrophils / Q000737:chemistry*; D013395:Sucrose
D003470:Culture Media; D013395:Sucrose; D005780:Gelatin