A gene-sized DNA molecule encoding the catalytic subunit of DNA polymerase alpha in the macronucleus of Oxytricha nova.
We have isolated a gene-sized molecule encoding the catalytic subunit of DNA polymerase alpha from a macronuclear genomic library of Oxytricha nova, by using a 0.7-kb fragment of the corresponding human gene as a hybridization probe. Two different versions of the gene are present in the macronucleus, one with an EcoRI site (RI+) and one without an EcoRI site (RI-). The cloned RI- version has been characterized. It is 4938 bp in length, excluding telomeres. It consists of a 329-bp 5' leader, a 4479-bp coding region and a 130-bp 3' trailer. The deduced amino-acid sequence shares conserved regions with the yeast and human polypeptides. We also demonstrate by Southern analysis that gene-sized molecules of similar size, homologous to the isolated O. nova gene are present in the mac genome of closely and distantly related hypotrichs.
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347.
S J
SJ
Mansour
D C
DC
Hoffman
D M
DM
Prescott
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D005075:Biological Evolution; D002467:Cell Nucleus / Q000201:enzymology*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004257:DNA Polymerase II / Q000235:genetics*; D016054:DNA, Protozoan; D006801:Humans; D008969:Molecular Sequence Data; D016813:Oxytricha / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
D016054:DNA, Protozoan; D004257:DNA Polymerase II