[Evaluation of semen quality after freezing at the temperature of liquid nitrogen using a modification of two different methods].
Spermatozoon survival rate was analysed after freezing at the temperature of liquid nitrogen (-196 degrees C) with the application of three different cryoprotector substances, vitelline and non-vitelline. A modified method, based on the use of sperm cells of small diameter, was introduced in freezing semen. The best results were achieved while using non-vitelline, multi-ionic cryoprotector. It was found out that glycerol alone does not protect semen from the effects of low temperature.
Ochedalski
T
T
I Kliniki Ginekologii Instytutu Ginekologii i Połoznictwa Akademii Medycznej, Lodzi.
Salata
I
I
Kowalski
A
A
Koprek-Kowalska
U
U
Karowicz-Bilińska
A
A
Czechowski
B
B
D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D006801:Humans; D008297:Male; D009584:Nitrogen; D013094:Spermatozoa
D003451:Cryoprotective Agents; D009584:Nitrogen
Ocena jakości nasienia po zamrozeniu w temperaturze ciekłego azotu przy zastosowaniu modyfikacji dwõch rõznych metod.