Cloning of a novel putative protein kinase having a leucine zipper domain from human brain.
We report a novel putative serine/threonine protein kinase containing a leucine-zipper domain, isolated from an human neuronal cell line. The teratocarcinoma cell line NT2 was differentiated to postmitotic NT2-N neurons by treatment with retinoic acid, and degenerate oligonucleotide primers to the catalytic domains of protein kinases were employed to PCR amplify subtractive cDNAs. We identified a clone, represented at higher abundance in NT2-N neurons than in the parental cell line, which encodes a putative serine/threonine kinase of 859 [corrected] amino acids, the leucine-zipper protein kinase (zpk). Zpk protein contains a leucine-zipper domain, found in many DNA-binding proteins, but few protein kinases. Steady-state mRNA levels for zpk are high in human brain and kidney. Further studies are required to evaluate the role of zpk in neuronal differentiation.
Reddy
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Children's Hospital of Philadelphia, PA 19104.
Pleasure
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D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D006801:Humans; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D009474:Neurons / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013724:Teratoma; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D017346:Protein Serine-Threonine Kinases