A new method for bovine pepsinogen purification. Preparation of a specific antibody.
Bovine pepsinogen was purified from abomasum by ammonium sulphate precipitation and ionic exchange chromatography on DEAE-cellulose and Mono Q columns. Purified pepsinogen was shown to be homogeneous by analytical electrophoresis, having an estimated molecular mass of 46,000 Daltons. The isoelectric point, determined by analytical chromatofocusing was 4.6. Using this pepsinogen preparation to immunize rabbits, a specific antiserum of high titer was obtained.
Department of Parasitology, Federal University of Minas Gerais, Brazil.
M A
MA
Gomes
W dos S
Wdos S
Lima
J L
JL
Pesquero
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D010435:Pepsinogens / Q000276:immunology* / Q000302:isolation & purification*
D000906:Antibodies; D010435:Pepsinogens