Effects of slaframine and 4-diphenylacetoxy-N-methylpiperidine methiodide (4DAMP) on pancreatic exocrine secretion in the bovine.
Three Holstein steers (345 +/- 22 kg) surgically fitted with a pancreatic cannula were used in two 3 x 3 Latin square design experiments to examine the effects of slaframine (SF), a muscarinic agonist, or 4-diphenylacetoxy-N-methylpiperidine methiodide (4DAMP), an M3 muscarinic glandular receptor antagonist, on pancreatic exocrine secretion. Pancreatic exocrine secretion was collected for 8 h postdosing at 30-min intervals beginning 1 h postfeeding. In experiment 1, steers were dosed with 0, 25, or 50 micrograms.kg-1 body weight (BW) of SF. Secretion of pancreatic juice and the pH of the secreted juice increased linearly (p < 0.05) with SF; however, secretion rate showed a time by treatment interaction (p < 0.05), as treatments converged 7 h postdosing. Trypsin secretion tended (p < 0.10) to show a quadratic response to SF administration, with the 25 micrograms SF.kg-1 BW dose having the lowest value. In experiment 2, steers received 50 micrograms.kg-1 BW of SF (positive control), 113 micrograms.kg-1 BW of 4DAMP (isosmolar with SF), or both. SF caused a greater pancreatic fluid secretion (p < 0.10) than 4DAMP, with SF plus 4DAMP intermediate. A time by treatment interaction (p < 0.04) was found, since treatments converged 8 h postdosing. Trypsin secretion was higher (p < 0.05) for SF than the other treatments. Chymotrypsin, alpha-amylase, and protein secretion were not affected. SF and 4DAMP alter pancreatic fluid secretion in the steer but have minimal effects on enzyme secretions.
Department of Animal Sciences, University of Kentucky, Lexington 40546-0215.
J A
JA
Walker
C R
CR
Krehbiel
D L
DL
Harmon
G
G
St Jean
W J
WJ
Croom
W M
WM
Hagler
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008297:Male; D018727:Muscarinic Antagonists; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism; D010276:Parasympatholytics / Q000494:pharmacology*; D010277:Parasympathomimetics / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism
D000470:Alkaloids; D018727:Muscarinic Antagonists; D010276:Parasympatholytics; D010277:Parasympathomimetics; D010880:Piperidines; D011506:Proteins; C009275:slaframine; C042375:4-diphenylacetoxy-1,1-dimethylpiperidinium; D000516:alpha-Amylases; D002918:Chymotrypsin