Anorectic responses to dietary amino acid imbalance: effects of vagotomy and tropisetron.
We investigated the roles of the vagus nerve and the serotonin3 (5-HT3) receptor in mediating the food intake depression associated with amino acid deficiency. The food intake of sham-operated (sham) rats given an isoleucine-imbalanced (IMB) diet was reduced to < 40% of control basal (BAS) diet intake (P = 0.0009), and pretreatment with the 5-HT3 antagonist tropisetron (Trop) increased IMB intake by twofold over the vehicle (VEH)-treated group (P < or = 0.0001), as we have reported before. However, after subdiaphragmatic vagotomy (VAGX), IMB intake was increased to a level intermediate between the sham-VEH and sham-Trop groups, while administration of Trop did not increase IMB intake over VAGX alone. By the end of day 1, the VAGX-Trop group had eaten only 1 g more of IMB than the VAGX-VEH group (NS). We conclude that 1) the vagus is among the physiological systems involved in the anorectic responses to IMB and 2) intact vagal function is necessary for the full effect of 5-HT3 antagonists in alleviating the anorectic responses to IMB.
Washburn
B S
BS
Department of Medicine, School of Veterinary Medicine, University of California, Davis 95616.
Jiang
J C
JC
Cummings
S L
SL
Dixon
K
K
Gietzen
D W
DW
D000818:Animals; D000856:Anorexia Nervosa / Q000209:etiology* / Q000503:physiopathology; D004032:Diet; D004435:Eating / Q000187:drug effects; D007211:Indoles / Q000494:pharmacology*; D007532:Isoleucine / Q000008:administration & dosage / Q000172:deficiency*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012702:Serotonin Antagonists / Q000494:pharmacology; D000077526:Tropisetron; D014628:Vagotomy
D007211:Indoles; D012702:Serotonin Antagonists; D007532:Isoleucine; D000077526:Tropisetron