[Impact on lymphocyte transformation with BCG different strains vaccination].
The authors compared the influence of BCG produced strains D1 and D2 vaccination on lymphocyte transformation of spleen lymphocytes from guinea pigs. The guinea pigs were divided into D1 strain vaccinated group, D2 strain vaccinated group and nonvaccinated group. The response of lymphocyte transformation of spleen lymphocytes from the three groups to the stimulation of PHA, ConA and PPD were compared at 3, 4, and 5 weeks following vaccination. It was shown that the lymphocyte proliferation of D2 strain vaccinated guinea pigs to the stimulation of PHA, ConA and PPD was higher than that of D1 strain vaccinated. The most sensitivity of immunized lymphocytes to PHA and ConA was at 4 weeks following vaccination. Lymphocytes proliferation to PPD at 5 weeks following vaccination was higher than that at 4 weeks. This study showed that the ability of D2 strain to elicit the CMI was more than that of D1 strain.
National Institute for the Control of Pharmaceutical and Biological Products, Beijing.
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D000818:Animals; D001500:BCG Vaccine / Q000145:classification / Q000276:immunology*; D002478:Cells, Cultured; D003208:Concanavalin A; D005260:Female; D006168:Guinea Pigs; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D013154:Spleen / Q000166:cytology; D014373:Tuberculin
D001500:BCG Vaccine; D014373:Tuberculin; D003208:Concanavalin A