Transplantation of hepatocytes: an in-vitro and in-vivo study in canines.
We isolated and transplanted hepatocytes in the canine, large animal model to evaluate hepatocyte yield and purity as well as the optimal site for hepatocyte engraftment (i.e., the spleen or the portal bed). We obtained viable, pure, single hepatocyte suspensions that were readily preserved at 4 degrees C in University of Wisconsin (UW) solution for up to 3 days. Both intrasplenic and portal vein injection were well tolerated, with minimal recipient morbidity and mortality when 1-2 x 10(9) hepatocytes were injected into immunosuppressed allogeneic hosts. We noted the embolization of hepatocytes into the parenchyma of the native liver within 7 days of intrasplenic transplantation that produced a mild reversible derangement of liver function and histology. These results warrant consideration prior to clinical trials of hepatocyte transplantation in man.
Rivas
P A
PA
Department of Surgery/Division of Transplantation, University of Illinois at Chicago 60680.
Fabrega
A J
AJ
Schwartz
D
D
Dagiantis
W
W
Ward
M G
MG
Blanchard
J
J
Pollak
R
R
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D004285:Dogs; D005978:Glutathione; D007328:Insulin; D008099:Liver / Q000166:cytology*; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000473:pathology / Q000502:physiology*; D019188:Organ Preservation Solutions; D011887:Raffinose; D013154:Spleen; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods; D016042:Transplantation, Heterotopic
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose