[A novel method for studying the free radical status of phagocytotic blood cells in patients with hemoglobinopathies].
Vital fluorochrome hydroethidine was used for measuring relative extra- and intracellular production of active oxygen in phagocytotic blood cells in hemoglobinopathies. Hydroethidine sensitivity to diverse free oxygen forms was studied in various model systems. Probability of false-positive results was estimated. It was established that hydroethidine interacts with superoxide anion radicals in molar ratio 1:1, that intracellular production of active oxygen forms in leukocytic mass cells stimulated by phorbol myristate acetate was enhanced in patients with major beta(+)-thalassemia and in a patient with hemoglobinopathy due to unstable hemoglobin Hb-Alesha. In thalassemia minor no significant differences compared to control were reported. Potential causes and value of the results obtained are under discussion.
Biziukin
A V
AV
Smetanina
N S
NS
Korkina
L G
LG
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005609:Free Radicals; D006453:Hemoglobinopathies / Q000097:blood*; D006801:Humans; D007223:Infant; D010587:Phagocytosis / Q000502:physiology*; D017382:Reactive Oxygen Species
D005609:Free Radicals; D017382:Reactive Oxygen Species
Novyĭ metod izucheniia svobodnoradikal'nogo statusa fagotsitiruiushchikh kletok krovi u patsientov s gemoglobinopatiiami.