Polymorphonuclear leukocyte-generated oxygen metabolites decrease beat frequency of human respiratory cilia.
We investigated the effect of polymorphonuclear leukocyte (PMN)-generated oxygen metabolites on the ciliary beat frequency. PMNs were incubated with human respiratory cilia obtained by nasal brushing. The oxidative metabolism was stimulated by opsonized zymosan, and ciliary beat frequency was evaluated before and after activation of PMNs. Ciliary beat frequency was studied using video microscopy. Our results demonstrate a significant decrease in ciliary beat frequency after activation of PMNs. This effect was reduced by catalase. These data suggest that the PMN-generated oxygen metabolites, particularly H2O2, decrease beat frequency of human respiratory cilia.
Department of Pediatrics, University of Ancona, Italy.
A
A
Kantar
N
N
Oggiano
P L
PL
Giorgi
P C
PC
Braga
R
R
Fiorini
D001205:Ascorbic Acid / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002648:Child; D002923:Cilia / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D009297:Nasal Mucosa / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D017382:Reactive Oxygen Species; D016897:Respiratory Burst; D013482:Superoxide Dismutase / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D017382:Reactive Oxygen Species; D015054:Zymosan; D002374:Catalase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid