Negative segregation of Mtv loci in H-2E+ mice selected for high antibody response.
Endogenous mouse mammary tumor proviruses (Mtvs) encode superantigens (Sags), which can delete T lymphocytes expressing particular Tcrb-V genes when associated with the H-2E molecule. In the present work, distribution of Mtvs was investigated in six independent pairs of mouse lines genetically selected for high (H) or low (L) Ab production to specific T-cell-dependent Ags. These experiments were performed to evaluate the role of Mtv-encoded Sags in the determination of H and L phenotypes. No systematic difference is observed in Mtv segregation between H-2E- H and L mouse lines. However, a clear differential segregation of Mtv loci is observed between H-2E+ H and L mice from three independent selections. The number of endogenous Mtvs in L lines is close to that found in laboratory mice. In contrast, Mtv loci-encoding Sags are almost absent in H animals. Although Sags profoundly affect the available Tcrb-V repertoire in H-2E+ L mice, it seems unlikely that Mtvs account for the L phenotype which can be achieved independently of Mtv segregation in H-2E- lines. More importantly, the results suggest that negative segregation of Mtv-encoding Sags contribute to determine the super-responder phenotype of H lines.
Roger
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T
Laboratorie d'Immunodifférenciation, Hall de Biotechnologies, Université Denis Diderot (Paris 7), France.
Boudaly
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Seman
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D000818:Animals; D000917:Antibody Formation / Q000235:genetics*; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006183:H-2 Antigens / Q000235:genetics*; D007107:Immune System; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011533:Proviruses / Q000235:genetics* / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis; D012641:Selection, Genetic; D018089:Superantigens / Q000235:genetics* / Q000276:immunology
D006183:H-2 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens