Differential proliferative and interleukin-10 responses to fractionated filarial antigens: preferential recognition by patients with chronic lymphatic dysfunction.
To characterize filarial antigens that may be associated with the development of chronic lymphatic dysfunction in persons with lymphatic filariasis, T cell responsiveness to Brugia pahangi adult worm extracts and SDS-PAGE antigen fractions were examined among Haitians from an area in which Wuchereria bancrofti is endemic. Greater T cell proliferation and interleukin-10 (IL-10) production were observed in amicrofilaremic patients with hydrocele or elephantiasis than in amicrofilaremic or microfilaremic asymptomatic persons. Antigen fractions that stimulated the highest proliferative responses (in the 25-49 kDa range) and IL-10 production were not identical. Further separation of an immunodominant 30- to 38-kDa fraction by ion exchange high-pressure liquid chromatography identified several subfractions, including a 32-kDa protein band, that elicited T cell responses from patients with elephantiasis or hydrocele. By immunoblot, these patients also had markedly greater humoral reactivity to parasite antigens of approximately 52, 43, 32, and 30 kDa.
Dimock
K A
KA
Division of Parasitic Diseases/F13, Centers for Disease Control and Prevention, Atlanta, GA 30341-3724.
Addiss
D G
DG
Eberhard
M L
ML
Lammie
P J
PJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D017179:Brugia pahangi / Q000276:immunology*; D005591:Chemical Fractionation; D002648:Child; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002908:Chronic Disease; D004605:Elephantiasis, Filarial / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D015151:Immunoblotting; D016753:Interleukin-10 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*
D000947:Antigens, Helminth; D016753:Interleukin-10