Expression of lactic dehydrogenase isoenzymes in rabbit muscle during development.
1. Rabbit cDNA probes for H and M lactic dehydrogenase subunits were used to monitor mRNA levels in different muscle types during growth. 2. At the same time, lactic dehydrogenase activity and relative quantities of H and M protein subunits were measured. 3. The main results are that mRNA abundance depends on muscle type and age, and mRNA abundance is not correlated with enzymatic activity.
Laboratoire de Biochimie, Université Blaise Pascal-Clermont II, Aubiere, France.
V L
VL
Marieze
M
M
Briand
S
S
Badaoui
M H
MH
Dadet
Y
Y
Briand
D000367:Age Factors; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D024510:Muscle Development; D009132:Muscles / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D007770:L-Lactate Dehydrogenase