Cloning and sequencing of the cDNA encoding human glutaredoxin.
Glutaredoxin (thioltransferase) is a small, heat-stable protein, which is involved in thiol/disulfide exchange reactions. We have isolated a cDNA that encodes glutaredoxin from a human brain cDNA library. The encoded protein contains 106 amino acids with a calculated molecular mass of 11.76 kDa and an isoelectric point of 8.09. The amino acid sequence deduced from the cDNA is more than 80% identical to those of other mammalian glutaredoxins.
Department of Biochemistry, Kyushu University School of Medicine, Fukuoka, Japan.
M R
MR
Fernando
H
H
Sumimoto
H
H
Nanri
S
S
Kawabata
S
S
Iwanaga
S
S
Minakami
Y
Y
Fukumaki
K
K
Takeshige
D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D054477:Glutaredoxins; D006801:Humans; D008969:Molecular Sequence Data; D010088:Oxidoreductases; D011506:Proteins / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D054477:Glutaredoxins; D011506:Proteins; D010088:Oxidoreductases