Variability within the Candida rugosa lipases family.
Several fungi secrete lipase isozymes differing in biochemical properties and in some cases in substrate specificity. In the yeast Candida rugosa, a family of related genes encodes for multiple lipase proteins, highly homologous in sequence but partially different in the regions interacting with the substrate molecule. Analysis of these substitutions performed on the basis of multiple alignments and using a 3-D model of the enzyme, allows identification of a restricted number of amino acids possibly involved in substrate specificity of Candida lipases.
Dipartimento Fisiologia e Biochimica Generali, Università degli Studi di Milano, Italy.
M
M
Lotti
A
A
Tramontano
S
S
Longhi
F
F
Fusetti
S
S
Brocca
E
E
Pizzi
L
L
Alberghina
D000595:Amino Acid Sequence; D002175:Candida / Q000201:enzymology*; D017124:Conserved Sequence; D014644:Genetic Variation; D007527:Isoenzymes / Q000235:genetics*; D008049:Lipase / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D007527:Isoenzymes; D008049:Lipase