Accumulation of unintegrated viral DNA in rat glial cells infected with neurotropic Friend murine leukemia virus.
FrC6 virus isolated from Friend murine leukemia virus (FLV) as a neurotropic virus clone induced a high-degree of accumulation of unintegrated viral DNA during infection of rat glial cells by 72 h post-infection. When anti-FLV neutralizing antibody, dextran sulfate, or 3'-azido-3'-deoxythymidine (AZT) was added to the culture within 24 h after infection, the accumulation of unintegrated viral DNA was inhibited. However, after 30-36 h post-infection, addition of anti-FLV antibody or dextran sulfate scarcely inhibited the accumulation of the unintegrated viral DNA, while addition of AZT at 30-36 h post-infection still reduced the amount of unintegrated viral DNA. Our results demonstrate that after 30-36 h post-infection, when second-round infection had already taken place, further superinfection with cell-free virus to glial cells was not required for the accumulation of unintegrated viral DNA. Possible mechanisms are discussed.
Institute of Life Science, Soka University, Tokyo, Japan.
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Takase-Yoden
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Watanabe
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000914:Antibodies, Viral / Q000494:pharmacology; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis*; D016264:Dextran Sulfate / Q000494:pharmacology; D005622:Friend murine leukemia virus / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D009457:Neuroglia / Q000382:microbiology*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D051381:Rats; D016662:Virus Integration / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects; D015215:Zidovudine / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D015215:Zidovudine; D016264:Dextran Sulfate