Thyroid hormone potentiates the antiviral action of interferon-gamma in cultured human cells.
Human interferon-gamma (hIFN gamma) exhibits a number of biological effects, including antiviral activity in homologous cells. The antiviral activity of recombinant (r) hIFN gamma, estimated by inhibition of vesicular stomatitis virus yield, was potentiated up to 120-fold in human fibroblast (BG-9) cells exposed to free L-T4 (0.5 x 10(-10) mol/L). Thyroid hormone alone did not induce the antiviral state in BG-9 cells. L-T3 also potentiated the antiviral action of rhIFN gamma, but D-T4 and D-T3 were ineffective. The antiviral effect of hIFN alpha in BG-9 cells was not influenced by thyroid hormone. Exposure of rhIFN gamma-treated BG-9 cells to L-T4 for only 3 h was sufficient to potentiate hIFN gamma-mediated antiviral activity. Similar potentiation by L-T4 of the antiviral effect of rhIFN gamma in HeLa cell cultures was also observed. Although the mechanism of potentiation of rhIFN gamma action by thyroid hormone is incompletely understood, the absence of antiviral activity of thyroid hormone alone indicates that the iodothyronine effect does not depend upon hormonal action on genes able to be stimulated by IFN gamma.
Department of Medicine, Albany Medical College, New York 12208.
H Y
HY
Lin
H R
HR
Thacore
P J
PJ
Davis
F B
FB
Davis
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004357:Drug Synergism; D005347:Fibroblasts / Q000382:microbiology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D011994:Recombinant Proteins; D013974:Thyroxine / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000254:growth & development
D000998:Antiviral Agents; D011994:Recombinant Proteins; D014284:Triiodothyronine; D007371:Interferon-gamma; D013974:Thyroxine