von Willebrand factor is not altered in azotemic dogs with prolonged bleeding time.
The effects of azotemia on von Willebrand factor (vWf) plasma concentration, structure, and function were studied by utilizing canine models for both uremic bleeding and type I vWf deficiency (vWd). Seventy-five percent to 80% renal mass reduction in eight mixed-breed dogs induced marked azotemia (blood urea nitrogen [BUN] 103 +/- 7 mg/dl [mean +/- SEM]; creatinine 5.8 +/- 1 mg/dl) and prolonged mean buccal mucosal bleeding time (BMBT) from 1.8 +/- 0.2 minutes to 7.0 +/- 0.4 minutes. The mean vWf plasma concentration increased from 0.88 +/- 0.11 U/ml to 1.26 +/- 0.14 U/ml. The pre- and postsurgical sodium dodecyl sulfate-agarose gel electrophoresis multimeric patterns were similar in all dogs. Administration of cryoprecipitate from pooled azotemic mixed-breed dog plasma to five Doberman pinschers with type I vWd increased the mean plasma vWf from 0.14 +/- 0.01 U/ml to 0.48 +/- 0.04 U/ml and decreased the BMBT from 7.1 +/- 0.6 minutes to 3.14 +/- 0.09 minutes. After renal mass reduction, five type I vWd Dobermans developed marked azotemia (BUN 79 +/- 8.6 mg/dl; creatinine 3.7 +/- 0.6 mg/dl) and prolonged BMBT (16.1 +/- 3.6 minutes). Findings in the eight azotemic mixed-breed dogs indicated that (1) vWf plasma levels were normal to increased in azotemic dogs; (2) vWf structure and multimeric distribution were not altered in canine azotemia; and (3) vWf was functional when placed in a non-azotemic environment. The prolongation of the BMBT in azotemic vWd dogs indicated that factors other than alteration of vWf function were responsible for the prolonged BMBT in canine azotemia.
Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California.
J A
JA
Brassard
K M
KM
Meyers
D000818:Animals; D001760:Bleeding Time; D001769:Blood; D011232:Chemical Precipitation; D003080:Cold Temperature; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology; D004285:Dogs / Q000097:blood; D005353:Fibronectins / Q000097:blood; D006487:Hemostasis; D009994:Osmolar Concentration; D012016:Reference Values; D014511:Uremia / Q000097:blood*; D014841:von Willebrand Factor / Q000032:analysis* / Q000737:chemistry
D005353:Fibronectins; D014841:von Willebrand Factor; D003894:Deamino Arginine Vasopressin