Characteristics of porin-like major outer membrane proteins of Listonella anguillara serotypes J-O-1, -2 and -3.
Major outer membrane protein (MOMP) was prepared from fish pathogen Listonella anguillara. Triton X-100 treatment could extract the MOMP from the bacterium but not from Escherichia coli, suggesting loose association of the MOMP of L. anguillara to the membrane. Properties of purified MOMP from L. anguillara were similar to Omp C porin of E. coli. Similar antigenicity of the porin like-MOMP was found among different serotypes of L. anguillara, although the molecular sizes of the MOMP were different among the strains.
Fish Disease Laboratory, Faculty of Agriculture, Kochi University, Japan.
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S
Suzuki
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K
Kuroe
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R
Kusuda
D000942:Antigens, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry; D008970:Molecular Weight; D018272:Porins / Q000737:chemistry* / Q000302:isolation & purification; D014461:Ultracentrifugation; D014733:Vibrio / Q000737:chemistry* / Q000145:classification
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018272:Porins