(CAC)5 detects DNA fingerprints and sequences homologous to gene transcripts in rice.
In the present report the potential of (CAC)5 is demonstrated for DNA fingerprinting in rice. Based on the fingerprint data, (CAC)5 was estimated to differentiate up to 6.3 x 10(8) genotypes of rice. In a search for homologous sequences in rice transcripts, four oligonucleotide probes, namely, (CAC)5, (GATA)4, (GACA)4, and (TG)10, were used. Among these probes, (CAC)5 showed hybridization to total rice RNA in a tissue-specific manner; while a band at 1.2 kb was common in both seed and leaf RNA, there were additional prominent bands at 1.9 and 2.3 kb in seed and leaf RNA, respectively. When (CAC)5 was hybridized to poly(A)+ RNA, in addition to a band at 1.2 kb in seed and leaf, hybridization was observed only in seed to heterogeneously sized RNAs.
Plant Molecular Biology Unit, National Chemical Laboratory, Pune, India.
V S
VS
Gupta
W
W
Ramakrishna
S R
SR
Rawat
P K
PK
Ranjekar
D001483:Base Sequence; D004247:DNA / Q000032:analysis; D016172:DNA Fingerprinting; D005838:Genotype; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000138:chemical synthesis; D012275:Oryza / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA