Regulation of oxidative degradation of L-lysine in rat liver mitochondria.
The generation of 14CO2 from [1-14C]lysine by hepatic mitochondria through the saccharopine pathway is controlled by intramitochondrial concentrations of lysine, 2-oxoglutarate and NADPH. Mitochondria, isolated from rats pre-treated with glucagon, exhibited higher activities of L-lysine: 2-oxoglutarate reductase, saccharopine dehydrogenase and 2-aminoadipate aminotransferase. The flux through this pathway is stimulated in liver mitochondria after glucagon treatment. Multiple regulation of lysine oxidation in liver mitochondria confirms a complex mechanism of 'mitochondrial activation' by glucagon.
Rowett Research Institute, Bucksburn, Aberdeen, U.K.
P W
PW
Scislowski
A R
AR
Foster
M F
MF
Fuller
D000818:Animals; D005934:Glucagon / Q000494:pharmacology*; D008239:Lysine / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D012443:Saccharopine Dehydrogenases / Q000378:metabolism*
D005934:Glucagon; D012443:Saccharopine Dehydrogenases; D008239:Lysine