Staphylococcal protein A containing phospholipid monolayers on aqueous and solid surfaces.
The staphylococcal protein A containing monolayer is characterized on both aqueous and solid surfaces. The density of SpA in dipalmitoylphosphatidic acid (DPPA) monolayers is determined from ultraviolet spectroscopy. The influence of calcium on the SpA concentration in the monolayer is also investigated. The spectroscopic results indicate that the incorporation of SpA in the monolayer is influenced by the calcium concentration in the subphase, and more than 90% of adsorbed protein is excluded from the monolayer when calcium concentration is in the order of millimoles. The IgG binding activity of the SpA in the reconstituted membrane is influenced by the surface pressure at which the membrane is formed.
Lu
B
B
Laboratory of Molecular and Biomolecular Electronics, Southeast Univ., Nanjing, P.R. China.
Yie
N
N
Wei
Y
Y
D002118:Calcium / Q000737:chemistry; D007074:Immunoglobulin G / Q000378:metabolism; D010712:Phosphatidic Acids / Q000737:chemistry*; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000378:metabolism; D014867:Water
D007074:Immunoglobulin G; D010712:Phosphatidic Acids; D013205:Staphylococcal Protein A; D014867:Water; C031599:dipalmitoylphosphatidic acid; D002118:Calcium