Vinca alkaloids enhance the half-life of tumour ornithine decarboxylase.
Naturally occurring vinca alkaloids, vincristine and vinblastine, at micromolar concentrations enhanced the ornithine decarboxylase activity in a dose-response manner in primary cultures of Ehrlich ascites carcinoma cells. After 8 h of culture in the presence or absence of vinblastine, cycloheximide was added to the medium, a 4.5-fold increase in the half-life of the ornithine decarboxylase activity was observed in vinblastine-treated cells. This effect is probably due to the inhibition of the intracellular enzyme degradation by the vinca alkaloids.
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.
P
P
Morata
J
J
Jiménez-Mesa
I
I
Núñez de Castro
F M
FM
Sánchez-Jiménez
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D006207:Half-Life; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D014748:Vinca Alkaloids / Q000494:pharmacology*
D014748:Vinca Alkaloids; D009955:Ornithine Decarboxylase