Production of 26-deoxymonensins A and B by Streptomyces cinnamonensis in the presence of Metyrapone.
Metyrapone, a potent cytochrome P-450 inhibitor, added at 9 mM to a submerged culture of Streptomyces cinnamonensis caused partial inhibition of total monensin biosynthesis and coproduction of new metabolites, 26-deoxymonensins A and B. The latter was isolated as its 25-O-methyl derivative. Metyrapone was simultaneously reduced to metyrapol. All of these compounds were identified by nuclear magnetic resonance spectroscopy and mass spectrometry.
Pospísil
S
S
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague.
Sedmera
P
P
Havlícek
V
V
Tax
J
J
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008797:Metyrapone / Q000031:analogs & derivatives / Q000378:metabolism; D008985:Monensin / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D011725:Pyridines / Q000378:metabolism / Q000494:pharmacology*; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D011725:Pyridines; C060472:26-deoxymonensin A; C088001:26-deoxymonensin B; C002196:metyrapol; C013109:metapyrone; D008985:Monensin; D008797:Metyrapone