Effects of isozyme-selective phosphodiesterase inhibitors on eosinophil infiltration in the guinea-pig lung.
The effects of selective phosphodiesterase isozyme inhibitors on eosinophil infiltration induced by antigen challenge or by exposure to an aerosol of platelet-activating factor (PAF) were investigated in the guinea-pig. Pretreatment 24 h and 3 h before antigen challenge or PAF exposure with theophylline (100 mg/kg), rolipram (5 mg/kg) and Ro 20-1724 (30 mg/kg) significantly reduced the increase in eosinophil numbers in bronchoalveolar lavage fluid. In contrast, milrinone, SK&F 94-120 and zaprinast were ineffective against antigen-induced eosinophil recruitment. Theophylline, rolipram and Ro 20-1724 also significantly reduced the release of eosinophil peroxidase into the bronchoalveolar lavage fluid. It is suggested that inhibitors of type IV phosphodiesterase have anti-inflammatory effects in the airways and may be useful in the treatment of asthma.
Laboratoire de Pharmacologie Expérimentale et Moléculaire, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Rennes I, France.
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V
Lagente
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I
Moodley
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Perrin
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Mottin
J L
JL
Junien
D000336:Aerosols; D000818:Animals; D000941:Antigens / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004804:Eosinophils / Q000187:drug effects / Q000201:enzymology; D006168:Guinea Pigs; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D008168:Lung / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008297:Male; D010544:Peroxidases / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000008:administration & dosage / Q000494:pharmacology; D011657:Pulmonary Eosinophilia / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology*
D000336:Aerosols; D000941:Antigens; D007527:Isoenzymes; D010726:Phosphodiesterase Inhibitors; D010972:Platelet Activating Factor; D010544:Peroxidases