A Na:H exchanger subtype mediates volume regulation in bovine corneal epithelial cells.
To identify a role for a Na:H (NHE) exchanger subtype in volume regulation in bovine corneal epithelium, we determined: 1. its sensitivity to inhibition by amiloride analogues 2. the effects of either Cl removal or hypertonicity on the intracellular pH. Our results suggest that volume regulatory responses elicited by stimulation of NHE-2 may help preserve epithelial barrier function in the face of increases in tear film osmolarity.
Reinach
P
P
Medical College of Georgia, Augusta 30912.
Ganapathy
V
V
Torres-Zamorano
V
V
D000584:Amiloride / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology / Q000502:physiology*; D004848:Epithelium / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D006982:Hypertonic Solutions; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000502:physiology*
D006982:Hypertonic Solutions; D017923:Sodium-Hydrogen Exchangers; D011189:Potassium Chloride; D000584:Amiloride