Simultaneous measurement of NK cell cytotoxicity against two target cell lines labelled with fluorescent lanthanide chelates.
We describe a cytotoxicity assay which permits the simultaneous measurement of natural killer cell activity against two different cell lines. The target cell lines are labelled either with a fluorescent europium chelate or with a fluorescent terbium chelate and cell death is quantified by measuring the chelate release. K-562, Molt4 and Daudi cell lines have been used as targets. The release of the two chelates from the target cells can be detected with the help of time resolved fluorometry. As the measurements are made after background fluorescence has decayed no additional steps are needed to correct for the background from the medium. The assay procedure used for measurement of cytotoxicity against two target cell lines is very similar to the widely used 51Cr release assay.
Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.
J
J
Lövgren
K
K
Blomberg
D002460:Cell Line; D002614:Chelating Agents; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods* / Q000706:statistics & numerical data; D005063:Europium; D005069:Evaluation Studies as Topic; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D008674:Metals, Rare Earth; D012680:Sensitivity and Specificity; D013696:Temperature; D013725:Terbium; D014407:Tumor Cells, Cultured / Q000276:immunology
D002614:Chelating Agents; D005456:Fluorescent Dyes; D008674:Metals, Rare Earth; D013725:Terbium; D005063:Europium