2,3-diphosphoglyceric acid blocks long-term potentiation of excitatory postsynaptic currents in hippocampal CA1 neurons of the rat.
The dependence of long-term potentiation on an intact inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] metabolism was investigated with the whole-cell voltage-clamp method in the CA1 region of hippocampal slices. The intracellular application of 2,3-diphosphoglyceric acid (1 mM), an inhibitor of the Ins(1,4,5)P3 5-phosphatase and of Ins(1,4,5)P3 3-kinase eliminated the potentiation of postsynaptic currents in pyramidal cells 30 min after paired pre- and postsynaptic activation. These data suggest a possible role of postsynaptic inositol 1,4-bisphosphate and/or inositol 1,3,4,5-tetra-kisphosphate in synaptic plasticity.
Institute for Neurobiology, Department of Neurophysiology, Magdeburg, FRG.
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Behnisch
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Reymann
D019794:2,3-Diphosphoglycerate; D000818:Animals; D004163:Diphosphoglyceric Acids / Q000494:pharmacology*; D005071:Evoked Potentials / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D000072181:Inositol Polyphosphate 5-Phosphatases; D017774:Long-Term Potentiation / Q000187:drug effects*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D017966:Pyramidal Cells / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D015544:Inositol 1,4,5-Trisphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase; D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases