Characterization of a synthetic 37-residue fragment of a monoclonal antibody against herpes virus by capillary electrophoresis/electrospray (ionspray) mass spectrometry and 252Cf plasma desorption mass spectrometry.
A peptide comprising 37 amino acids of the antigen binding site of a monoclonal antibody directed against glycoprotein D of herpes simplex virus was synthesized. The synthetic peptide and the impurities formed in the synthesis were characterized by capillary electrophoresis/ionspray mass spectrometry and by 252Cf plasma desorption-time of flight mass spectrometry. The measured average molecular mass of the synthetic peptide was 4627.16 Da, which was only 0.08 Da higher than the calculated value (4627.08 Da). The plasma desorption mass spectrum of the synthetic peptide showed a protonated molecule at m/z 4624.1, which was 4 Da lower than the calculated one (4628.09 Da). The amino acid sequence of the peptide was confirmed in part by electrospray (ionspray) mass spectrometry using a high nozzle skimmer voltage difference. Five impurities were separated and identified by capillary electrophoresis/mass spectrometry and two of them also appeared in the plasma desorption mass spectrum.
University Centre for Pharmacy, Groningen, The Netherlands.
R
R
Kostiainen
E
E
Lasonder
W
W
Bloemhoff
P A
PA
van Veelen
G W
GW
Welling
A P
AP
Bruins
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D002142:Californium; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007128:Immunoglobulin Fragments / Q000032:analysis*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011946:Receptors, Antigen / Q000737:chemistry; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007128:Immunoglobulin Fragments; D011946:Receptors, Antigen; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; D002142:Californium