In vitro antimicrobial and cytotoxic effects of Kri 1 paste and zinc oxide-eugenol used in primary tooth pulpectomies.
The antimicrobial and cytotoxic effects of Kri 1 paste, an iodoform-based primary tooth filling material, were compared with zinc oxide-eugenol (ZOE), using in vitro techniques. Antimicrobial evaluation involved measuring inhibition zones of Streptococcus faecalis on brain heart agar. Cytotoxicity evaluation involved direct cell-medicament contact experiments of 4-hr and 24-hr duration using fresh and set medicaments, and indirect cell-medicament contact experiments of 24-hr duration using fresh and set medicaments. ZOE produced a greater zone of bacterial inhibition than Kri 1 paste. Kri 1 paste cytotoxicity remained high regardless of the amount of setting time in the 4-hr direct contact experiment, while ZOE cytotoxicity decreased with setting time. Both Kri 1 paste and ZOE had high cytotoxicity regardless of setting time in the 24-hr direct cell-medicament contact test. ZOE cytotoxicity decreased to control levels after only 1 day of setting in the indirect contact experiments, compared with greater than 7 days for Kri 1 paste. The results suggest ZOE has better antimicrobial activity than Kri 1 paste. ZOE also has lower cytotoxicity, although prolonged cell-medicament contact may result in both medicaments having similarly high cytotoxicity.
Department of Pediatric Dentistry and Orthodontics, University of Connecticut Health Center, Farmington.
K J
KJ
Wright
S V
SV
Barbosa
K
K
Araki
L S
LS
Spångberg
D000704:Analysis of Variance; D000818:Animals; D002164:Camphor / Q000494:pharmacology / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D013293:Enterococcus faecalis / Q000187:drug effects*; D005347:Fibroblasts / Q000502:physiology; D006847:Hydrocarbons, Iodinated / Q000494:pharmacology / Q000633:toxicity*; D051379:Mice; D008826:Microbial Sensitivity Tests; D012387:Root Canal Filling Materials / Q000494:pharmacology / Q000633:toxicity*; D014094:Tooth, Deciduous; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology / Q000633:toxicity*
D006847:Hydrocarbons, Iodinated; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C036374:Kri paste; D002164:Camphor