Interactions of polyamines in the measurement of free magnesium concentration by mag-fura-2 and 31P-NMR.
Polyamines, particularly spermine, in physiological concentrations interact with mag-fura-2 and the mag-fura-2/Mg2+ complex, resulting in reduced values of free Mg2+ concentration. Similarly, polyamines interact with ATP and MgATP. Thus, free Mg2+ concentration, as measured by 31P-NMR or mag-fura-2, is underestimated in the presence of polyamines, particularly of spermine.
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany.
T
T
Günther
J
J
Vormann
P
P
Konstanczak
A
A
Schäfer
D000255:Adenosine Triphosphate / Q000737:chemistry; D016257:Fura-2 / Q000031:analogs & derivatives*; D008274:Magnesium / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D011073:Polyamines / Q000737:chemistry*
D011073:Polyamines; C058776:2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid; D000255:Adenosine Triphosphate; D008274:Magnesium; D016257:Fura-2