Vitamin E prevents glucose-induced lipid peroxidation and increased collagen production in cultured rat mesangial cells.
High glucose directly promotes lipid peroxidation and stimulates collagen production by cultured mesangial cells. We conducted the following experiments to ascertain whether vitamin E, a lipophilic antioxidant agent, could reverse these effects in vitro. Rat mesangial cells were grown to confluence and then maintained for 7 additional days in control media (DME) containing 5.6 mM glucose or experimental media containing 33.3 mM glucose. The test condition resulted in a near doubling of mesangial cell lipid peroxidation, assessed by measuring the content of conjugated dienes. High-glucose media also enhanced collagen production by 28% above the basal level in control media. Addition of vitamin E (100 microM) reversed both effects of the elevated ambient glucose level and prevented lipid peroxidation and normalized collagen production in cultured mesangial cells. These findings suggest that vitamin E functions as an endogenous antioxidant agent in the kidney to limit the development of glomerulosclerosis in diabetic nephropathy.
Department of Pediatrics, Schneider Children's Hospital, Long Island Campus for the Albert Einstein College of Medicine, New Hyde Park, New York 11042.
H
H
Trachtman
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000037:antagonists & inhibitors*; D015227:Lipid Peroxidation / Q000187:drug effects*; D051381:Rats; D014810:Vitamin E / Q000494:pharmacology*
D014810:Vitamin E; D003094:Collagen; D005947:Glucose