Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin.
Gender identification of forensic samples was determined by amplifying a segment of the X-Y homologous gene amelogenin. Using a single pair of primers spanning part of the first intron, 106 bp and 112 bp PCR products were generated from the X and Y homologues respectively, which were then resolved by agarose gel electrophoresis. This test enabled as little as 20 pg of DNA from severely degraded bones to be amplified and typed in a single tube reaction. Furthermore, using dye-labelled primers, it was possible to quantitate, by automated fluorescence detection, the relative yields of X and Y-specific PCR products generated from mixtures of male and female DNA. The versatility of this sex test was further demonstrated by co-amplifying with the HLA-DQA1 Amplitype kit in a combined gender/identity DNA test.
Istituto di Medicina Legale, Universita' di Genova, Italy.
A
A
Mannucci
K M
KM
Sullivan
P L
PL
Ivanov
P
P
Gill
D000483:Alleles; D053523:Amelogenin; D001842:Bone and Bones / Q000378:metabolism; D002874:Chromosome Mapping; D003746:Dental Enamel Proteins / Q000235:genetics*; D004190:Disasters; D005260:Female; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011180:Postmortem Changes; D017385:Sequence Homology; D012732:Sex Determination Analysis / Q000379:methods*; D014960:X Chromosome; D014998:Y Chromosome
D053523:Amelogenin; D003746:Dental Enamel Proteins; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen