The decreased transfer of arachidonic acid from triglycerides to phospholipids during proliferation of a human gastric tumor (HGT) cell line is not linked to modification of membrane fluidity.
Prelabeling-chase experiments with [14C]arachidonic acid in human cancer gastric cells (HGT) indicated a preferential incorporation of arachidonic acid into triglycerides followed by a transfer to phospholipids. This phenomenon was quicker using cells in log phase growth than in quiescent phase and was not linked to modification of HGT membrane fluidity assessed by the fluorescence polarization of the 1,6-diphenyl hexatriene probe. By contrast, growing cells in a medium enriched with oleic or linoleic acids increase plasma membrane fluidity. The importance of the site of arachidonic incorporation in HGT lipid is discussed.
Laboratoire d'Immuno-Hémato-Biochimie, Pôle Recherche GCA, Groupe Guerbet, Chasse sur Rhône, France.
A
A
Najid
D
D
Es-Saady
Y
Y
Denizot
D016718:Arachidonic Acid / Q000378:metabolism*; D002455:Cell Division; D006801:Humans; D008560:Membrane Fluidity; D010743:Phospholipids / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014280:Triglycerides / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D010743:Phospholipids; D014280:Triglycerides; D016718:Arachidonic Acid