Adenovirus-mediated gene transfer to murine retinal cells in vitro and in vivo.
Adenovirus-mediated gene transfer to retinal cells was evaluated using the replication-defective recombinant adenovirus vector Ad2/CMVlacZ-1 (coding for beta-galactosidase) both in an in vitro murine culture model and in vivo in adult mice. In vitro, no difference in infectability of neuronal and glial cells was observed, and 50% of neurons expressed the exogenous gene at low viral concentration (10 pfu/cell). In vivo, intraocular injection of 3 x 10(6) pfu Ad2/CMVlacZ-1 resulted in expression of the transferred beta-galactosidase gene in retinal pigment epithelium and ganglion cells. These results demonstrate that Ad2/CMVlacZ-1 is an effective vector for gene transfer into retinal cells.
Department of Pharmacology, Rayne Institute, UMDS, Guy's, London, UK.
C
C
Jomary
T A
TA
Piper
G
G
Dickson
L A
LA
Couture
A E
AE
Smith
M J
MJ
Neal
S E
SE
Jones
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D016900:Neurofilament Proteins / Q000032:analysis; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics*
D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins; D001616:beta-Galactosidase