The use of fed batch cultivation for achieving high cell densities in the production of a recombinant protein in Escherichia coli.
The production of the fusion protein staphylococcal protein A/E. coli beta-galactosidase in Escherichia coli was studied in batch and fed batch cultivations. Batch cultivation of a recombinant E. coli strain yielded a final cell dry weight of 16.4 g l-1 with a final intracellular product concentration of recombinant protein corresponding to approximately 38% of the cell dry weight. Fed batch cultivation made it possible to increase the final cell dry weight to 77.0 g l-1. The intracellular product concentration (25%) was lower as compared to batch cultivation resulting in a total concentration of recombinant protein of 19.2 g l-1.
Strandberg
L
L
Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
Andersson
L
L
Enfors
S O
SO
D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D010101:Oxygen Consumption; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000235:genetics; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D001616:beta-Galactosidase; D005947:Glucose