Near-tetraploid poorly differentiated acute myeloid leukemia M0 diagnosed by short-term cultures with a phorbol ester TPA.
Leukemic blasts of two patients with acute leukemia exhibited similar characteristics. They were heterogeneous in size with a diameter of 14-30 microns in smears and unclassifiable by morphological, cytochemical, immunophenotypic and ultrastructural examinations. Cytogenetic examinations of both revealed a near-tetraploid karyotype. Blasts from both patients differentiated into macrophages in cultures with 10 ng/ml 12-O-tetradecanoylphorbol-13-acetate (TPA) which is a feature specific for myeloid blasts and the cases were thus classified as poorly differentiated acute myeloid leukemias (AML M0). Near-tetraploid poorly differentiated acute myeloid leukemias M0 seem to be a special category of AML in the morphologic, immunologic and cytogenetic (MIC) classification. The presence of very large blasts in the heterogeneous blast population in acute unclassified leukemias could be a morphological sign of near-tetraploid leukemias AML M0.
Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
P
P
Lemez
J
J
Jelínek
K
K
Michalová
K
K
Koubek
J
J
Schwarz
V
V
Malasková
B
B
Rypácková
A
A
Jirásek
J
J
Brezinová
J
J
Hrabánek
D000368:Aged; D002454:Cell Differentiation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011123:Polyploidy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004273:DNA, Neoplasm; D013755:Tetradecanoylphorbol Acetate