[A new thermophilic strain of Bacillus coagulans--a producer of BcoK1 site-specific endonuclease].
The strain, producing new site-specific endonuclease BcoKI has been found at the screening of the thermophilic bacteria isolated from tobacco. A phenotype characteristic of the strain is given. It has been identified as a new strain Bacillus coagulans. BcoKI, a class-IIS restriction endonuclease has been obtained by three consecutive chromatographies on blue agarose hydroxyapatite and heparin-Sepharose. BcoKI, an isoschizomer of Ksp6321, recognizes the six base non-palindromic sequence 5'CTCTTC3' and cleaves one nucleotide 3' of the 3' cytosine on this strand and four nucleotide 5' of the 5' guanine on the opposite strand to generate a three base 5' overhang.
Kovalevskaia
N P
NP
Zheleznaia
L A
LA
Zelinskaia
N V
NV
Matvienko
N I
NI
D001345:Autoradiography; D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D013379:Substrate Specificity
C057073:endodeoxyribonuclease Ksp632I; D015252:Deoxyribonucleases, Type II Site-Specific
Novyĭ termofil'nyĭ shtamm Bacillus coagulans--produtsent saĭt- spetsificheskoĭ éndonukleazy BcoKI.