The effects of transcervical monofilament insertion on the microbial status of the uterus in guinea-pigs.
The monofilament marker tail attached to intra-uterine contraceptive devices (IUCDs) has been implicated in the development of pelvic infection because it acts as a substrate for bacterial adhesion, and facilitates the transmission of vaginal bacteria into the uterus. A guinea-pig model was used to investigate the role of transcervical monofilaments in the transmission of vaginal bacteria into the uterus. By 21 days the degree of uterine contamination was significantly lower than after 24 h (P < 0.05), but was still significantly greater than control values (P < 0.01). Coating the monofilaments with poly (2-hydroxyethyl-methacrylate) (polyHEMA) had no effect on uterine bacterial counts at 24 h. After 21 days, however, contamination within the uteri fitted with polyHEMA-coated monofilaments had significantly increased from levels observed at 24 h (P < 0.01). These counts were also significantly greater than those recorded for the uncoated and control groups at the same time interval. In animals fitted with monofilaments, in which chlorhexidine had been incorporated into the polyHEMA coating, the level of uterine contamination after 24 h was significantly lower than that observed with uncoated threads at the same time interval (P < 0.02). After 21 days there was no significant difference between uterine bacterial counts from the chlorhexidine group and control animals. It is concluded that the use of such drug-loaded monofilaments offers the potential to minimize infections associated with the transcervical insertion of IUCDs.
Department of Pharmacy, King's College London, UK.
J
J
Bilbruck
G W
GW
Hanlon
P R
PR
Gard
G P
GP
Martin
D000818:Animals; D001419:Bacteria / Q000187:drug effects; D002710:Chlorhexidine / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D007434:Intrauterine Devices / Q000009:adverse effects*; D009757:Nylons; D011102:Polyhydroxyethyl Methacrylate; D014599:Uterus / Q000382:microbiology*
D009757:Nylons; D011102:Polyhydroxyethyl Methacrylate; D002710:Chlorhexidine