Aberrant expression of the monocyte/macrophage phenotype in a human T cell line immortalized by HTLV-I and an adult T cell leukemia/lymphoma cell line.
An HTLV-I-immortalized human T cell line (JP-2), a N-methyl-N'-nitro-N-nitrosoguanidine-treated JP-2 line (JP-2T), and an adult T cell leukemia cell line (ATL-1T) were examined morphologically and phenotypically. All of these cell lines expressed some T cell markers, including CD4, and showed rearrangement of T cell receptor (TCR) genes, but they lacked CD3 and TCR antigens and expressed some myelomonocytic markers (CD68, HL-21, CD15, CD16). JP-2 cells grew in suspension, but JP-2T and ATL-1T cells, which mostly adhered to the surface of culture vessels, showed macrophage-like morphological features and expressed more monocyte/macrophage markers (lysozyme, alpha 1-antitrypsin) and fibronectin. ATL-1T cells transplanted into SCID mice lost the macrophage features. These results suggest that HTLV-I infected T cells can express some macrophage features and that these cells may provide a model that will be useful in elucidating the phenotypic variability of T cell lymphomas.
Jeon
H J
HJ
Second Department of Pathology, Okayama University Medical School, Japan.
Akagi
T
T
Yoshino
T
T
Takahashi
K
K
Hayashi
K
K
Kondo
E
E
Sarker
A B
AB
Teramoto
N
N
Fujiwara
K
K
Ohara
N
N
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D015139:Blotting, Southern; D002461:Cell Line, Transformed / Q000276:immunology / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007231:Infant, Newborn; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000276:immunology / Q000473:pathology*; D008264:Macrophages / Q000276:immunology / Q000473:pathology*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008854:Microscopy, Electron; D009000:Monocytes / Q000276:immunology / Q000473:pathology*; D009368:Neoplasm Transplantation; D010587:Phagocytosis / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000473:pathology
D015703:Antigens, CD; D013755:Tetradecanoylphorbol Acetate