One of the retinoic acid-inducible cDNA clones in mouse embryonal carcinoma F9 cells encodes a novel isoenzyme of fructose 1,6-bisphosphatase.
Rae-30, one of the retinoic acid (RA)-inducible cDNA clones in mouse embryonal carcinoma F9 cells, was sequenced and the deduced RAE-30 protein showed about a 70% homology to mammalian fructose 1,6-bisphosphatase (EC 3.1.3.11) (FBPase), in comparison to over 85% homology observed among the previously documented rat liver, pig kidney and human leukemic HL-60 cell FBPases. The Rae-30 mRNAs were not detected in various tissues of adult mice, including the liver and kidney, but were detected in a placenta and predominantly in the intestine of adult mice. These findings indicate that the Rae-30 cDNA encodes a novel isoenzyme of FBPase, which is likely to be involved in early differentiation in mammalian cells.
Department of Medical Genetics, Osaka University, Japan.
M
M
Nomura
Y
Y
Takihara
T
T
Yasunaga
K
K
Shimada
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D018236:Carcinoma, Embryonal / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006597:Fructose-Bisphosphatase / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D014212:Tretinoin; D006597:Fructose-Bisphosphatase