Detection of calcitonin gene expression in human infant and monkey carotid body chief cells by in situ hybridization.
Calcitonin mRNA was detected in human and monkey carotid bodies by in situ hybridization histochemistry, using a 35S-labeled oligonucleotide probe for human calcitonin. In both human and monkey carotid body, moderate to high hybridization signal for calcitonin mRNA was observed in all cases. The hybridization signal in the formalin-fixed, paraffin-embedded samples was comparable to that obtained from frozen paraformaldehyde-fixed tissue. Our observations extend the finding of calcitonin-like immunoreactivity in the carotid body chief cells and indicate that calcitonin is produced in the carotid body, probably in the chief cells.
Department of Pathology, Hospital for Sick Children, Toronto, Ontario, Canada.
Y Y
YY
Wang
E
E
Cutz
D G
DG
Perrin
D000818:Animals; D001483:Base Sequence; D002116:Calcitonin / Q000096:biosynthesis* / Q000235:genetics; D002344:Carotid Body / Q000166:cytology* / Q000378:metabolism; D002675:Child, Preschool; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D007223:Infant; D007231:Infant, Newborn; D008252:Macaca fascicularis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D013045:Species Specificity
D002116:Calcitonin