14-3-3 protein homologs required for the DNA damage checkpoint in fission yeast.
During the cell cycle, DNA is replicated and segregated equally into two daughter cells. The DNA damage checkpoint ensures that DNA damage is repaired before mitosis is attempted. Genetic studies of the fission yeast Schizosaccharomyces pombe have identified two genes, rad24 and rad25, that are required for this checkpoint. These genes encode 14-3-3 protein homologs that together provide a function that is essential for cell proliferation. In addition, S. pombe rad24 null mutants, and to a lesser extent rad25 null mutants, enter mitosis prematurely, which indicates that 14-3-3 proteins have a role in determining the timing of mitosis.
Ford
J C
JC
Research Centre, King Faisal Specialist Hospital, Riyadh, Saudi Arabia.
al-Khodairy
F
F
Fotou
E
E
Sheldrick
K S
KS
Griffiths
D J
DJ
Carr
A M
AM
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins; D002455:Cell Division; D004249:DNA Damage; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D004260:DNA Repair; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D005800:Genes, Fungal; D047908:Intracellular Signaling Peptides and Proteins; D008938:Mitosis; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment; D015398:Signal Transduction; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D029702:Schizosaccharomyces pombe Proteins; C501114:rad24 protein, S pombe; C489574:rad25 protein, S pombe; D014446:Tyrosine 3-Monooxygenase; D004265:DNA Helicases