Cloning and correct expression in E. coli of the petJ gene encoding cytochrome c6 from Synechocystis 6803.
Cytochrome c6 from the cyanobacterium Synechocystis 6803 has been isolated and purified to electrophoretic homogeneity. The gene coding for such a heme protein (petJ) has been cloned and properly expressed in E. coli. This procedure yields a protein preparation completely identical to that obtained from the cyanobacterial cells. The N-terminal amino acid sequences of cytochrome c6 synthesized in both organisms are the same, thus allowing us to conclude that the petJ gene product is correctly processed in E. coli. To the best of our knowledge, this is the first time that any cytochrome c6 is produced in the enterobacterium. The identical physicochemical and kinetic properties of the proteins isolated from both sources confirm that expression of the petJ gene in E. coli is an adequate tool to address the study of Synechocystis cytochrome c6 by site-directed mutagenesis in a parallel way to that carried out with plastocyanin from the same organism.
Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla, Spain.
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Díaz
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Navarro
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Hervás
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Chávez
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Florencio
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De la Rosa
D001483:Base Sequence; D015139:Blotting, Southern; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics*; D003580:Cytochromes / Q000737:chemistry / Q000235:genetics*; D045348:Cytochromes f; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010970:Plastocyanin / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D013053:Spectrophotometry
D003580:Cytochromes; D015342:DNA Probes; D011994:Recombinant Proteins; D010970:Plastocyanin; D045348:Cytochromes f