vanA-mediated vancomycin-resistant Enterococcus spp. in sewage.
Three high-level vancomycin-resistant Enterococcus strains (two Enterococcus faecium and one Enterococcus durans) were recovered from three of eight sewage samples taken from the general sewage collector at Logroño (Northern Spain). The strains were present in the sewage samples at estimated concentrations of ten resistant bacteria/mL, corresponding to about 0.4% of the enterococcal population. The VanA protein was detected in each strain by immunoblotting of membrane extracts of the vancomycin-induced cells, and the vanA gene was demonstrated in the wild strains and their transconjugants by DNA-DNA hybridization. This is the first, confirmed report of vanA mediated vancomycin resistance in E. durans.
Area de Bioquímica y Biología Molecular, Universidad de la Rioja, Logroño, Spain.
C
C
Torres
J A
JA
Reguera
M J
MJ
Sanmartin
J C
JC
Pérez-Díaz
F
F
Baquero
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D019729:Carbon-Oxygen Ligases; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial / Q000235:genetics; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics; D008025:Ligases / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012722:Sewage; D014640:Vancomycin / Q000494:pharmacology*; D014871:Water Microbiology
D001426:Bacterial Proteins; D012722:Sewage; C071437:VanA ligase, Bacteria; D014640:Vancomycin; D008025:Ligases; D019729:Carbon-Oxygen Ligases