[Urea sensor for the continuous ammonium-selective enzymatic process control of the artificial kidney].
Presented is a flow-through method for continuous ammonium-selective enzymatic monitoring of the artificial kidney by means of a bioelectrochemical urea electrode. The urea is converted in an enzyme membrane by covalently bound urease and the ammonium ions are detected by a Nonactin-PVC-membrane. In addition to detailed data from the oxygen-independent solid-state contact sensor, curves are obtained on-line from the patient during the haemodialysis session. The advantages of the method are described in detail. Furthermore, the urea sensor can be used for measurements in heparinized blood.
Institut für Normale und Pathologische Physiologie, Philipps-Universität, Marburg/Lahn, Germany.
J G
JG
Schindler
M M
MM
Schindler
K
K
Herna
E
E
Reisinger
B
B
Burk
U
U
Kuhlmann
J
J
Knaack
B
B
Schmidt
H
H
Lange
D000900:Anti-Bacterial Agents; D015374:Biosensing Techniques; D004566:Electrodes; D006801:Humans; D007688:Kidneys, Artificial; D018942:Macrolides; D008567:Membranes, Artificial; D011143:Polyvinyl Chloride; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D006435:Renal Dialysis; D014508:Urea / Q000097:blood*; D014510:Urease
D000900:Anti-Bacterial Agents; D018942:Macrolides; D008567:Membranes, Artificial; D000644:Quaternary Ammonium Compounds; D014508:Urea; D011143:Polyvinyl Chloride; D014510:Urease; C050476:nonactin
Harnstoff-Durchflussensor zur ammoniumselektiv-enzymatischen Prozessknotrolle Prozesskontrolle der Künstlichen Niere.