[The isolation and characteristics of monoclonal antibodies to the structural proteins of Coxsackie B5 virus].
Five hybridomas secreting monoclonal antibodies (MCA) to Coxsackie B5 virus were generated by fusion of Sp2/0 myeloma cells with BALB/c mouse immune splenocytes. Most MCA belonged to the IgG and one to the IgM class. Out of 4 MCA binding the structural protein VP2, two MCA showed type specificity in neutralization test and cross-reactivity with other enteroviruses, one MCA reacted with all enteroviruses tested, and the one showing type specificity among Coxsackie B group "recognized" an epitope on poliovirus type 1 as well. The MCA belonging to VP4 proved to be Coxsackie B group specific. It was assumed that MCA could be used for studies of the antigenic structure and serodifferentiation of Coxsackie B5 virus.
A
A
Boss
A
A
Piirsoo
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000276:immunology; D029822:Enterovirus B, Human / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D015151:Immunoblotting; D007132:Immunoglobulin Isotypes / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D015678:Viral Structural Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007132:Immunoglobulin Isotypes; D015678:Viral Structural Proteins
Poluchenie i kharakteristika monoklonal'nykh antitel k strukturnym belkam virusa Koksaki B5.