Inhibitory effect of karounidiol on 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion.
Karounidiol [D:C-Friedo-oleana-7,9(11)-diene-3 alpha,29-diol] and 7-oxodihydrokarounidiol [7-oxo-D:C-friedo-olean-8-ene-3 alpha,29-diol], isolated from the seeds of Trichosanthes kirilowii, were examined for the effect on 12-O-tetradecanoylphorbol-13-acetate (TPA, 1 microgram/ear)-induced inflammation, following application of this tumor-promoting agent, to the ears of mice. Both compounds inhibited the inflammatory activity induced by TPA and the 50% inhibitory dose for TPA-induced inflammation was 0.3 and 0.4 mg/ear, respectively. Furthermore, at 2 mumol/mouse, karounidiol markedly suppressed the promoting effect of TPA (1 microgram/mouse) on skin tumor formation in mice following initiation with 7,12-dimethylbenz[a]anthracene (50 micrograms/mouse).
College of Pharmacy, Nihon University, Chiba, Japan.
K
K
Yasukawa
T
T
Akihisa
T
T
Tamura
M
M
Takido
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D003043:Cocarcinogenesis; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control; D051379:Mice; D008813:Mice, Inbred ICR; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D010212:Papilloma / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D012878:Skin Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*; D014315:Triterpenes / Q000494:pharmacology*
D016588:Anticarcinogenic Agents; D014315:Triterpenes; C088116:karounidiol; C076913:7-oxodihydrokarounidiol; D015127:9,10-Dimethyl-1,2-benzanthracene; D009828:Oleanolic Acid; D013755:Tetradecanoylphorbol Acetate