cDNA and deduced amino acid sequences of a human colon dihydrodiol dehydrogenase.
A 1.24 kbp cDNA encoding a human colon HT29 cell dihydrodiol dehydrogenase of 323 amino acid residues (M(r) 36,735) has been isolated and sequenced. The deduced amino acid sequence was 97%, 81%, and 76% identical to human liver dihydrodiol dehydrogenase (also called bile acid binder), human liver chlordecone reductase, and bovine lung prostaglandin f synthase, respectively. It was 98% identical in 990 bp overlap with the nucleotide sequence of human liver pseudochlordecone reductase. Northern blots revealed that dihydrodiol dehydrogenase(s) were markedly induced in HT29 colon cells by treatment with the Michael acceptor agent ethacrynic acid.
Ciaccio
P J
PJ
Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, PA 19111.
Tew
K D
KD
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003106:Colon / Q000201:enzymology*; D003110:Colonic Neoplasms / Q000201:enzymology; D018076:DNA, Complementary / Q000737:chemistry*; D004790:Enzyme Induction / Q000187:drug effects; D004976:Ethacrynic Acid / Q000494:pharmacology; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000737:chemistry; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008969:Molecular Sequence Data; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D017385:Sequence Homology; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C023125:dihydrodiol dehydrogenases; D006911:Hydroxyprostaglandin Dehydrogenases; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; C031051:prostaglandin-F synthase; C048321:chlordecone reductase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C116788:cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase; D004976:Ethacrynic Acid