[Radioimmunoimaging with monoclonal antibody to HBV X protein in nude mice bearing human hepatocellular carcinoma].
A mouse monoclonal antibody directed against the protein of hepatitis B virus X open reading frame was prepared. After radiolabeled with sodium 131I-iodine and administration, imaging was performed with gamma camera and radioactivity of the tumor, organs, and blood were counted in a mini-gamma counter at day 1, 3, 5, 7 in nude mice bearing HCC xenografts, and the biodistribution and T: NT ratio was calculated. Selective accumulation was observed in tumor sites on day 3 and a clear image of tumor was shown in gamma camera on day 7. The image of tumor in the irrelevant IgG group was not obtained at any time. The tumor:blood ratio was 0.48 and tumor:liver was 1.2 on day 1, then increased to 1.5 and 4.5 on day 7 in experimental group, respectively. Specific mAb uptake by tumor was significantly greater than nonspecific IgG(P < 0.05) on day 7. The results were encouraging. The finding of this study indicates the possibility of using 131I-anti-HBx mAb to target cytostatic drugs to HCC.
Liver Cancer Institute, Shanghai Medical University.
J
J
Li
Z Y
ZY
Tang
K D
KD
Liu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000302:isolation & purification; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging* / Q000473:pathology; D006511:Hepatitis B Antigens / Q000276:immunology*; D007457:Iodine Radioisotopes; D008113:Liver Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016719:Radioimmunodetection; D015534:Trans-Activators / Q000276:immunology*; D054334:Viral Regulatory and Accessory Proteins
D000911:Antibodies, Monoclonal; D006511:Hepatitis B Antigens; D007457:Iodine Radioisotopes; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein