Pharmacokinetic variables and bioavailability from muscle of creatine kinase in cattle.
Pharmacokinetic variables of skeletal muscle creatine kinase (CK) activity after IV administration of a muscle extract; CK bioavailability after IM administration of the muscle extract; and effect of IM administration of saline solution, to appreciate the possible release of CK consecutive to muscle puncture, were determined in 6 cows. A general equation for the quantitative estimation of skeletal muscle damage also was derived. Administration of saline solution IM had no effect on plasma CK activity (ANOVA, P > 0.05) in any of the cows. After IV administration of the muscle extract (150 U/kg of body weight), mean volume of the central compartment, plasma half-life, and plasma clearance of CK were 0.027 +/- 0.007 L/kg, 520 +/- 109 minutes, and 6.43 +/- 2.29 ml/kg/h, respectively. After IM administration (150 U/kg), mean bioavailability of CK was 51 +/- 17% and maximal plasma CK activity (500 +/- 97 U/L) was observed at 454 +/- 131 minutes. The rate of CK activity entry into plasma was determined by use of deconvolution analysis. Two peaks were observed; the first appeared before the 30th minute after IM administration, and the second appeared at 3.3 +/- 1.1 hours. Amplitudes were 6.31 +/- 4.45 and 6.57 +/- 3.08 U/kg/h, for the first and the second peaks, respectively. The quantity of CK liberated from control muscle was 0.69 +/- 0.12 U/kg/h, corresponding to a normal daily catabolism of 5.8 +/- 1.0 mg of muscle/kg.(ABSTRACT TRUNCATED AT 250 WORDS)
Département de Physiopathologie, Ecole National Vétérinaire, Toulouse, France.
H P
HP
Lefebvre
P L
PL
Toutain
J P
JP
Serthelon
V
V
Lassourd
L
L
Gardey
J P
JP
Braun
D000704:Analysis of Variance; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D003402:Creatine Kinase / Q000493:pharmacokinetics*; D005260:Female; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D009132:Muscles / Q000201:enzymology* / Q000473:pathology; D012965:Sodium Chloride; D014020:Tissue Extracts
D014020:Tissue Extracts; D012965:Sodium Chloride; D003402:Creatine Kinase