Hybridization of strains of Escherichia coli O157 with probes derived from the eaeA gene of enteropathogenic E. coli and the eaeA homolog from a Vero cytotoxin-producing strain of E. coli O157.
A total of 375 Escherichia coli O157 strains were tested by colony hybridization with the eae probe from the central portion of the eaeA gene of the classical enteropathogenic E. coli strain E2348/69. They were also tested with a probe, eaeO157, from the C-terminal end of the eae gene homolog from a Vero cytotoxin (VT)-producing strain of E. coli (VTEC) of serotype O157:H7. Both probes hybridized with all 246 O157:H7 or H- VTEC strains tested. The majority were from human infections, and the remainder were from cattle. A further 10 strains (H7 or H-) hybridized with both eae and eaeO157 sequences but not with VT probes. They resembled O157 VTEC and were probably naturally occurring derivatives that had lost VT genes. The remaining 119 strains of O157 were from human, animal, and food sources and belonged to 16 H types other than H7 or were H-. They were VT negative and differed in their properties from O157 VTEC: generally they fermented sorbitol in 1 day, produced beta-glucuronidase, and could not be phage typed by the scheme for O157 VTEC. The eae probe but not the eaeO157 sequence hybridized with 18 H8 or H39 strains, predominantly from human diarrhea. The remaining 101 VT-negative strains hybridized with neither probe. However, 16 strains of O157:H45 hybridized with a probe for diffusely adherent E. coli and attached to HEp-2 cells in a diffuse pattern. Serogroup O157 comprises strains with heterogeneous properties. The eaeO157 probe is a valuable addition to the VT probes used to differentiate O157 strains.
Willshaw
G A
GA
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, United Kingdom.
Scotland
S M
SM
Smith
H R
HR
Cheasty
T
T
Thomas
A
A
Rowe
B
B
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012703:Serotyping; D022622:Shiga Toxin 1; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
D001427:Bacterial Toxins; D015342:DNA Probes; D004269:DNA, Bacterial; D022622:Shiga Toxin 1