Experimental evaluation of Celsior, a new heart preservation solution.
An original heart preservation solution (Celsior) has been developed, the formulation of which has been designed to fulfil two major objectives: (1) to combine the general principles of hypothermic organ preservation with those specific for the myocardium, and (2) to offer the possibility of being used not only as a storage medium but also as a perfusion fluid during initial donor heart arrest, poststorage graft reimplantation and early reperfusion. The major principles addressed by the Celsior formulation include (1) prevention of cell swelling (by mannitol and lactobionate), (2) prevention of by the Celsior formulation include (1) prevention of cell swelling (by mannitol and lactobionate), (2) prevention of oxygen-derived free radical injury (by reduced glutathione, histidine and mannitol), and (3) prevention of contracture by enhancement of energy production (glutamate) and limitation of calcium overload (high magnesium content, slight degree of acidosis). Two experimental preparations were used: The isolated isovolumic buffer-perfused rat heart model and the heterotopic rabbit heart transplantation model. In isolated heart experiments, hearts were arrested with and stored in Celsior for 5 h at 4 degrees C and subsequently reperfused for 1 h. A similar protocol was used in the transplantation experiments except that the total ischemic time was approximately 1 1/2 h longer (corresponding to 6 h of storage followed by the 25 additional minutes of cold ischemia required for graft implantation.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Cardiovascular Surgery, Hôpital Lariboisière, Paris.
P
P
Menasché
J L
JL
Termignon
F
F
Pradier
C
C
Grousset
C
C
Mouas
G
G
Alberici
M
M
Weiss
A
A
Piwnica
G
G
Bloch
D000005:Abdomen; D000818:Animals; D001639:Bicarbonates; D002122:Calcium Chloride; D002314:Cardioplegic Solutions; D003080:Cold Temperature; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D005978:Glutathione; D016027:Heart Transplantation / Q000502:physiology; D006639:Histidine; D008274:Magnesium; D008297:Male; D008353:Mannitol; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D009926:Organ Preservation / Q000379:methods*; D011189:Potassium Chloride; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride; D013997:Time Factors; D016042:Transplantation, Heterotopic
D001639:Bicarbonates; D002314:Cardioplegic Solutions; C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; C041711:St. Thomas' Hospital cardioplegic solution; D008353:Mannitol; D012965:Sodium Chloride; D006639:Histidine; D011189:Potassium Chloride; D005978:Glutathione; D008274:Magnesium; D002122:Calcium Chloride