A competitive enzyme immunoassay Subclass for the determination of total IgG-subclass levels in human serum. Comparison with single radial immunodiffusion.
A quantitative immunoassay has been developed for the determination of the total IgG subclass levels in human serum. The method is based on an enzyme immunoassay in which IgG subclass proteins IgGx in an unknown serum sample compete with a known quantity of peroxidase (PO)-labelled IgGx in fluid phase for subclass-specific monoclonal antibodies coated to the solid phase of microtiter wells. Using a series of human blood samples an excellent correlation was observed with the IgG-subclass levels determined by single radial immunodiffusion.
Research Group C.B.F.LETI, Tres Cantos (Madrid), Spain.
F
F
Marañón
M
M
Casanovas
L
L
Berrens
J M
JM
Ollés
M A
MA
Dieguez
D001667:Binding, Competitive; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification; D007132:Immunoglobulin Isotypes / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D011933:Reagent Kits, Diagnostic