Heterologous systems for expression of mammalian sulfotransferases.
This paper describes the use of both mammalian and bacterial expression systems as tools to study the structural and functional relationships of proteins encoded by cDNAs to both rat and human aryl sulfotransferases. In particular, we describe the use of the mammalian COS cell system for functional expression studies, and the use of Escherichia coli for the expression and purification of a sulfotransferase fusion protein suitable as an antigen for the generation of sulfotransferase antibodies.
Department of Clinical Pharmacology, Flinders University of South Australia, Adelaide.
M E
ME
Veronese
W
W
Burgess
D
D
Cruickshank
L
L
Sansom
X
X
Zhu
M E
ME
McManus
D000818:Animals; D000917:Antibody Formation; D015239:Arylsulfotransferase / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D015239:Arylsulfotransferase