Susceptibility to copper-enhanced autoxidation of VLDL+LDL fractions from diabetic patients.
We describe a simplified method for studying the susceptibility to in vitro autoxidation of lipoproteins containing apolipoprotein B. It comprises copper induced autoxidation of VLDL+LDL plasma fractions and an assay for thiobarbituric acid reactive soluble substances. We studied autoxidation of VLDL+LDL in a population of 30 healthy subjects and a population of 30 diabetic patients. No significant difference in susceptibility to autoxidation could be detected. Protection afforded by ascorbic acid amounted to more than 95% at concentrations easily attainable in vivo by oral supplementation. Thiobarbituric acid reactive substances were higher in plasma from diabetic patients: 0.51 +/- 0.25 mumol/l vs 0.35 +/- 0.07 mumol/l for control subjects (p < 0.001). Our data confirm the presence of higher levels of lipid peroxides in diabetic patients, but fail to demonstrate any difference in susceptibility to oxidation of apolipoprotein B containing particles between control and diabetic subjects.
Département d'Immunologie, Immunopharmacologie et Pathologie, Faculté de Pharmacie, Université Louis Pasteur, Illkirch, France.
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Gugliucci
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Menini
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Stahl
D001055:Apolipoproteins B / Q000378:metabolism; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology*; D019327:Copper Sulfate; D003920:Diabetes Mellitus / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper; D019327:Copper Sulfate; D001205:Ascorbic Acid