Solution properties of the capsular polysaccharide produced by Klebsiella pneumoniae K40.
This paper reports some physicochemical properties of the capsular polysaccharide produced by Klebsiella pneumoniae serotype K40 (K40-CPS) in aqueous solution. The polymer has a linear hexasaccharide repeating unit containing one glucuronic acid residue as the only ionizable group. Potentiometric, viscometric, chiro-optical and rheological measurements have been carried out over a range of ionic strength, pH and temperature, with the aim of characterizing the conformational state of the polysaccharide in aqueous solution. All the data reported indicate that the K40-CPS does not undergo a cooperative conformational transition under the investigated experimental conditions. Furthermore, the viscosity data and the viscoelastic spectra suggest that the K40-CPS is rather flexible and adopts a random coil conformation in solution.
Centro Ricerche POLY-biòs, LBT-AREA di Ricerca, Trieste, Italy.
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Flaibani
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Leonhartsberger
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Navarini
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Cescutti
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Paoletti
D002240:Carbohydrate Sequence; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000737:chemistry*; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012212:Rheology; D012996:Solutions; D013696:Temperature; D014783:Viscosity
D011135:Polysaccharides, Bacterial; D012996:Solutions