Identification of DNA segments capable of rescuing a non-mendelian mutant in paramecium.
The non-Mendelian mutant d48 of Paramecium tetraurelia contains micronuclear wild type A genes, but at autogamy and conjugation proper processing fails and new macronuclei lack A genes. When cloned A genes are injected into the macronucleus of d48, proper processing is restored at the next autogamy; d48 is rescued, becoming permanently wild type. In the present study we have injected portions of the A gene into d48. We find that the ability to rescue extends over a large portion of the gene, with highest activity near a series of 221-bp repeat units in the middle of the gene. Regions outside the A gene are inactive.
Kim
C S
CS
Department of Biology, Indiana University, Bloomington 47405.
Preer
J R
JR
Polisky
B
B
D000818:Animals; D000953:Antigens, Protozoan; D000954:Antigens, Surface / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D017125:Genes, Protozoan; D009154:Mutation; D010247:Paramecium / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C043323:immobilization antigens