[Detection by PCR of genomic markers in canine parvovirus and feline panleukopenia virus].
The NS1 and VP1/VP2 genes of canine parvovirus and feline panleukopenia virus were amplified by the polymerase chain reaction (PCR). The restriction cleavage patterns of the amplified DNA fragments were compared among these parvoviruses including vaccine strains. Differences of the restriction site in the NS1 portions were observed between the vaccine strain and the wild type strain as well as between canine parvoviruses and feline panleukopenia viruses. The restriction patterns of feline panleukopenia viruses were distinct from those of canine parvoviruses, and showed differences between the vaccine strain and its wild type strain. This PCR-based restriction cleavage can be used for ecological study of such viruses.
Centre zoologique pour Recherches biomédicales, Faculté de Médecine, Université de Tokyo, Japan.
R
R
Harasawa
M
M
Yoshida
Y
Y
Kataoka
H
H
Katae
D028301:Feline Panleukopenia Virus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D005819:Genetic Markers; D066298:In Vitro Techniques; D017993:Parvovirus, Canine / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014765:Viral Vaccines; D014774:Virulence
D005819:Genetic Markers; D014765:Viral Vaccines
Détection par PCR de marqueurs génomiques dans le parvovirus canin et le virus de la panleucopénie féline.