Molecular cloning of a bovine MSH receptor which is highly expressed in the testis.
A novel G protein-coupled receptor (BDF3) was isolated from bovine genomic DNA by a combined approach of polymerase chain reaction (PCR) and hybridization techniques. The predicted amino acid sequence is 317 amino acids in length and displays 80% homology to the human alpha-MSH receptor MC1. Stably transfected into CHO-K1 cells, BDF3 mediates an increase of intracellular cAMP-levels following incubation with NLE-alpha-MSH, a potent alpha-MSH analog. The stimulation with ACTH1-10 is only moderate and gamma-MSH is ineffective. Northern blot analysis of bovine tissues revealed that the BDF3 gene is highly expressed in the testis as a single 2.3 kb mRNA species, suggesting an involvement of the BDF3 receptor in spermatogenesis.
Physiologisches Institut, Ludwig Maximilian Universität München, Germany.
M
M
Vanetti
C
C
Schönrock
W
W
Meyerhof
V
V
Höllt
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002417:Cattle; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011979:Receptors, Pituitary Hormone / Q000737:chemistry / Q000235:genetics*; D017385:Sequence Homology; D013737:Testis / Q000378:metabolism*; D014162:Transfection
D011979:Receptors, Pituitary Hormone; C029446:MSH receptor; D004247:DNA; D019204:GTP-Binding Proteins