Naive T cells can mediate delayed-type hypersensitivity response in T cell receptor transgenic mice.
We produced transgenic mice expressing T cell receptor-alpha beta chain genes, derived from the chicken ovalbumin (OVA)-specific I-Ad-restricted CD4+CD8- T helper cell clone 7-3-7. In transgenic mice with H-2d genetic background (Tg-d mice), delayed-type hypersensitivity (DTH) was induced in the hind footpad by one inoculation with OVA without any previous sensitization, suggesting that naive T cells have the potential to be involved in DTH response. Spleen cells from nonimmunized Tg-d mice showed a strong T cell proliferative response to in vitro stimulation with OVA. Furthermore, these spleen cells produce cytokines including interleukin(IL)-2, IL-3, interferon-gamma, granulocyte/macrophage colony-stimulating factor, macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta, which may play an important role in the attraction of mononuclear cells to an antigen-challenging site.
Department of Immunology, Tokai University School of Medicine, Kanagawa, Japan.
T
T
Sato
T
T
Sasahara
Y
Y
Nakamura
T
T
Osaki
T
T
Hasegawa
T
T
Tadakuma
Y
Y
Arata
Y
Y
Kumagai
M
M
Katsuki
S
S
Habu
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis*; D005434:Flow Cytometry; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007156:Immunologic Memory; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
D016207:Cytokines; D016693:Receptors, Antigen, T-Cell, alpha-beta