Protein kinase C rapidly up-regulates the number of AT1 angiotensin receptors on cultured rat intestinal epithelial (RIE-1) cells.
Addition of 12-O-tetradecanoylphorbol-13-acetate to RIE-1 rat intestinal epithelial cells stimulated a rapid (mean 3-fold) increase in the subsequent binding of 125I-labelled angiotensin II which was reversed or prevented when cellular protein kinase C was depleted. The increased binding was due, in part, to an up-regulation in the number of AT1 angiotensin receptors on RIE-1 cells, without any significant change in their binding affinity. Since this rapid up-regulation was independent of receptor synthesis, it may result from an increased availability (to extracellular ligand) of performed, but previously 'cryptic', AT1 angiotensin receptors.
Smith
R D
RD
Department of Clinical Biochemistry, Addenbrooke's Hospital, Cambridge, UK.
D000818:Animals; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011945:Receptors, Angiotensin / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015854:Up-Regulation
D011945:Receptors, Angiotensin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate