IL-10 inhibits HIV-1 replication and is induced by tat.
Interleukin 10 (IL-10) is produced by TH2 lymphocytes and regulates both lymphoid and myeloid cells. In the present study we demonstrate that IL-10 is expressed and produced spontaneously in the peripheral blood mononuclear cells (PBMCs) of all HIV-1 infected individuals tested, 3 of 19 cases of HIV-negative lymphoma and none of five healthy controls. IL-10 mRNA was detectable in both monocytes/macrophages and T lymphocytes isolated from PBMCs of HIV infected patients. We have also shown that infection of promonocytic (U937) and T (H9) cell lines with HIV stimulates IL-10 secretion. Furthermore, a T cell line (H9) stably transfected with a HIV tat expression-vector secreted higher levels of IL-10. We have also demonstrated that rhIL-10 inhibited HIV-1 replication in infected monocytes and PBMCs in a dose dependent manner. IL-10 may thus participate in long latency between HIV-1 infection and development of AIDS.
Department of Medicine, University of Southern California, Los Angeles 90033.
R
R
Masood
Y
Y
Lunardi-Iskandar
T
T
Moudgil
Y
Y
Zhang
R E
RE
Law
C L
CL
Huang
R K
RK
Puri
A M
AM
Levine
P S
PS
Gill
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D015870:Gene Expression; D015696:Gene Products, tat / Q000378:metabolism*; D015756:Genes, tat; D018023:HIV Seronegativity; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000494:pharmacology* / Q000502:physiology*; D008223:Lymphoma / Q000097:blood / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012016:Reference Values; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014162:Transfection; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology*; D054322:tat Gene Products, Human Immunodeficiency Virus
D017931:DNA Primers; D015696:Gene Products, tat; D012333:RNA, Messenger; D054322:tat Gene Products, Human Immunodeficiency Virus; D016753:Interleukin-10