Frequency of sister chromatid exchanges in humans at 14-16 week foetal stage and at birth.
In order to identify transplacental induced mutagenicity in humans the baseline frequency of sister chromatid exchanges (SCEs) in amniotic fluid cells (8.56 +/- 0.28 per cell) from 14 to 16-week-old foetuses as well as in peripheral blood lymphocytes (7.96 +/- 0.26 per cell) from newborns was determined. A group of twelve patients who were analysed both at 14-16 weeks gestation and at birth showed no SCE variations. These results suggest that foetal amniocytes and T lymphocytes from newborns present a similar sensitivity to the mutagenic activity of the thymidine analogue 5-bromodeoxyuridine employed in the sister chromatid differentiation.
Goyanes
V
V
Sección de Genética, Centro Materno-Infantil Teresa Herrera, La Coruña, Spain.
Fernández
J L
JL
Pereira
S
S
Campos
A
A
Rodriguez
E
E
González
M
M
Rodriguez
M
M
Espido
O
O
García
M
M
D000328:Adult; D000653:Amniotic Fluid / Q000166:cytology; D002875:Chromosomes / Q000648:ultrastructure; D005260:Female; D005333:Fetus / Q000166:cytology / Q000648:ultrastructure; D006801:Humans; D007231:Infant, Newborn; D007743:Labor, Obstetric; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D012854:Sister Chromatid Exchange; D013601:T-Lymphocytes / Q000166:cytology / Q000648:ultrastructure