Effects of Ca channel agonists on 45Ca uptake differ depending on the state of NG108-15 cells.
We investigated the effects of various Ca channel agonists (Ca agonist) derived from 1,4-dihydropyridine on KCl-stimulated 45Ca uptake by differentiated and undifferentiated neuroblastoma x glioma hybrid NG108-15 cells with and without dibutyryl cAMP. Ca agonists Bay K 8644, YC-170 and CGP 28392 enhanced KCl-stimulated 45Ca uptake in differentiated NG108-15 cells, but only slightly in undifferentiated NG108-15 cells. The rank order of the enhancing effects was roughly Bay K 8644 > YC-170 > > CGP 28392. These results suggest that there is some difference between the mechanism by which these Ca agonists affect KCl-stimulated 45Ca uptake in differentiated and undifferentiated NG108-15 cells, although the nature of that difference is not clear.
Department of Biological Chemistry, Faculty of Pharmacy, Kinki University, Higashi-Osaka, Japan.
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S
Ichida
T
T
Wada
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N
Matsuda
S
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Nakazaki
D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000493:pharmacokinetics*; D002120:Calcium Channel Agonists / Q000493:pharmacokinetics*; D002132:Calcium Radioisotopes; D002454:Cell Differentiation; D005910:Glioma; D006801:Humans; D006822:Hybrid Cells / Q000187:drug effects* / Q000378:metabolism*; D009447:Neuroblastoma; D011189:Potassium Chloride / Q000494:pharmacology; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D002120:Calcium Channel Agonists; D002132:Calcium Radioisotopes; D003994:Bucladesine; D011189:Potassium Chloride; D002118:Calcium