Trichinella spiralis: recovery of newborn larvae from cardiac tissues.
Newborn larvae (NL) of Trichinella spiralis in cardiac tissues were recovered by incubating cardiac tissue blocks of mice and gerbils infected with T. spiralis in RPMI- 1640 medium supplemented with antibiotics and 5% FCS for 24 h in a humidified atmosphere of 5% CO2 in air at 37 degrees C. This technique is effective for the recovery of migrating NL in visceral tissues which are en route to skeletal muscles, and may help to clarify the involvement of NL in the pathogenesis of cardiac or cerebral lesions in trichinellosis.
Kumagai
M
M
Department of Parasitology, Hirosaki University School of Medicine, Aomori, Japan.
Kamiya
H
H
D000818:Animals; D005260:Female; D005849:Gerbillinae; D006321:Heart / Q000469:parasitology*; D007814:Larva / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D017160:Trichinella spiralis / Q000302:isolation & purification* / Q000502:physiology; D014235:Trichinellosis / Q000469:parasitology*