[Urea sensor for the continuous ammonium-selective enzymatic process control of the artificial kidney].
Presented is a flow-through method for continuous ammonium-selective enzymatic monitoring of the artificial kidney by means of a bioelectrochemical urea electrode. The urea is converted in an enzyme membrane by covalently bound urease and the ammonium ions are detected by a Nonactin-PVC-membrane. In addition to detailed data from the oxygen-independent solid-state contact sensor, curves are obtained on-line from the patient during the haemodialysis session. The advantages of the method are described in detail. Furthermore, the urea sensor can be used for measurements in heparinized blood.
Schindler
J G
JG
Institut für Normale und Pathologische Physiologie, Philipps-Universität, Marburg/Lahn, Germany.
Schindler
M M
MM
Herna
K
K
Reisinger
E
E
Burk
B
B
Kuhlmann
U
U
Knaack
J
J
Schmidt
B
B
Lange
H
H
D000900:Anti-Bacterial Agents; D015374:Biosensing Techniques; D004566:Electrodes; D006801:Humans; D007688:Kidneys, Artificial; D018942:Macrolides; D008567:Membranes, Artificial; D011143:Polyvinyl Chloride; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D006435:Renal Dialysis; D014508:Urea / Q000097:blood*; D014510:Urease
D000900:Anti-Bacterial Agents; D018942:Macrolides; D008567:Membranes, Artificial; D000644:Quaternary Ammonium Compounds; D014508:Urea; D011143:Polyvinyl Chloride; D014510:Urease; C050476:nonactin
Harnstoff-Durchflussensor zur ammoniumselektiv-enzymatischen Prozessknotrolle Prozesskontrolle der Künstlichen Niere.