Molecular characterisation of cDNA clones representing pectin esterase isozymes from tomato.
Two pectin esterase cDNA clones representing different isozymes with ca. 95% homology were isolated from an early ripening tomato fruit cDNA library. Both clones were longer than previously published sequences, and the encoded proteins possessed extended (229-233 amino acid) putative N-terminal extensions. In addition, the mRNA species corresponding to the two clones showed differential levels of expression in fruit.
Hall
L N
LN
Department of Physiology and Environmental Science, University of Nottingham, UK.
Bird
C R
CR
Picton
S
S
Tucker
G A
GA
Seymour
G B
GB
Grierson
D
D
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014675:Vegetables / Q000201:enzymology / Q000235:genetics*
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase