[Establishment and characterization of a SV40 transformed human fetal gastric epithelial cell line-GES-1].
9 month human fetal gastric epithelial cells were primarily cultured and infected with SV40 virus. About 3 weeks after infection the transformed-like cells showed up and were isolated when most of the normal cells senesced. One transformed clone named GES-1 were analyzed biologically. The results indicated integration of the SV40 T gene and its expression in the cell nuclei and the cells became hyperploid in chromosomes. This cells also maintained a normal cytoskeleton, positive in PAS reaction and were non-tumorigenic in nude mice. The established cell line may be served as an important model system in study of human gastric carcinogenesis.
Ke
Y
Y
Department of Cytogenetics, Beijing Institute for Cancer Research.
Ning
T
T
Wang
B
B
D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004847:Epithelial Cells; D005333:Fetus; D005753:Gastric Mucosa / Q000166:cytology*; D015870:Gene Expression; D005814:Genes, Viral; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D013539:Simian virus 40 / Q000235:genetics; D016662:Virus Integration