Alteration of the T cell repertoire after bone marrow transplantation.
We investigated T cell receptor (TCR) alpha/beta and gamma/delta repertoire reconstitution after autologous and allogeneic bone marrow transplantation (BMT) in humans using 13 monoclonal antibodies directed at constant and variable regions of the TCR. The TCR gamma/delta repertoire was studied kinetically during the first month and then 1 year post-BMT whereas alpha/beta peripheral blood T lymphocytes (PBTL) were studied within the first 3 months and 1 year post-BMT. Through these two studies, we found 7 of 26 patients with over-represented TCR gamma/delta subsets during the early period post-BMT. Moreover, during this period the V gamma 9V delta 2 frequency among gamma/delta T cells was found to be higher than among normal donors. Study on TCR alpha/beta T cells also revealed abnormally expanded V-specific subset (5 of 10 patients within 3 months following BMT) demonstrating that repertoire alteration early after BMT is a general phenomenon concerning potentially all T cell subsets. More surprisingly, the alpha/beta T cell repertoire was also found to be altered late after BMT (7 of 15 patients after 1 year post-BMT presented one or more overepresented alpha/beta TCR subset). These alterations of TCR combinatorial diversity should be taken into account in understanding the immunological status of patients after BMT.
INSERM U211 and CHR Nantes, Institut de Biologie, France.
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D
Villers
N
N
Milpied
J
J
Gaschet
F
F
Davodeau
M M
MM
Hallet
M
M
Bonneville
H
H
Vié
D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002648:Child; D005260:Female; D015870:Gene Expression; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000276:immunology / Q000601:surgery; D007958:Leukocyte Count; D008223:Lymphoma / Q000235:genetics / Q000276:immunology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics / Q000276:immunology / Q000601:surgery; D010641:Phenotype; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta