Urinary growth hormone determination in prepubertal children using a modification of a commercial kit designed for determination of growth hormone in serum.
The aims of the present study were to validate a method for the determination of growth hormone (GH) in urine using a commercial immunoradiometric kit designed for serum GH determination and to derive a reference range for healthy prepubertal school children. The urinary GH concentrations in prepubertal children with short stature undergoing provocative GH tests were also investigated. Intra- and interassay coefficients of variance were 5.9% and 4.3% respectively and the mean detection limit of the assay, calculated as +2SD from the blank values was 0.2 microU l-1. Significant differences in the levels of urinary GH were observed between different collection times in the same individual. The urinary GH excretion in the normal school children was 8.2 +/- 4.5 microU night-1 (range 1.3-19.6 microU night-1). The urinary GH excretion in the short healthy children was not significantly lower than that of the healthy school children. No correlation was found between height, growth velocity or body-mass index and the overnight excretion of GH in the normal school children.
Department of Pediatrics, Orebro Medical Centre Hospital, Sweden.
J
J
Aman
I
I
Jones
D001827:Body Height; D002648:Child; D003404:Creatinine / Q000652:urine; D005260:Female; D013006:Growth Hormone / Q000097:blood / Q000652:urine*; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic; D013006:Growth Hormone; D003404:Creatinine