Development of cowpea mosaic virus as a high-yielding system for the presentation of foreign peptides.
It has recently been shown that cowpea plants can be infected with a cowpea mosaic virus (CPMV) chimera containing an antigenic site from foot-and-mouth disease virus (Usha et al., Virology 197, 366-374, 1993). Analysis of progeny RNA produced during such an infection has revealed that the inserted sequence is rapidly lost during serial passaging, probably by a process of homologous recombination. Using the information gained from this analysis, we have redesigned the chimeras in such a way that they are now genetically stable. The modified constructs have been used to obtain large quantities of purified virus particles expressing epitopes derived from human rhinovirus 14 (HRV-14) and human immunodeficiency virus type 1 (HIV-1). The chimeric virus particles possess the antigenic properties of the inserted sequence and, in the case of the HRV-14-derived construct, it has been shown that the inserted epitope is immunogenic in rabbits. These results demonstrate that CPMV can be used as a high-yielding system for the presentation of foreign peptide sequences.
Department of Virus Research, John Innes Institute, Norwich, United Kingdom.
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Porta
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Spall
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Loveland
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Johnson
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Barker
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Lomonossoff
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000276:immunology; D017798:Comovirus / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D012229:Rhinovirus / Q000276:immunology
D000956:Antigens, Viral; D015700:HIV Envelope Protein gp41; D010455:Peptides; D011993:Recombinant Fusion Proteins