Morphological transformation of Syrian hamster embryo cells by pseudorabies virus related growth factor.
Syrian hamster embryo (SHE) clonal morphological transformation assay has been used for the in vitro testing of transforming activity of Pseudorabies virus (PRV) related growth factor (PRGF). It was found that PRGF induces morphologically transformed colonies of SHE cells in the wide titer scale (in the range 1 x 10(7) to 1 x 10(13) U/ml). The concentrations of PRGF which induced the transforming phenotype of SHE cells in the colonies did not cause significant cytotoxic effect.
Department of Mutagenesis and Chemical Carcinogenesis, Slovak Academy of Sciences, Bratislava.
M
M
Dusinskaá
J
J
Lesko
F
F
Golais
D
D
Slamenová
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D006133:Growth Substances / Q000494:pharmacology*; D011558:Herpesvirus 1, Suid; D008647:Mesocricetus / Q000196:embryology; D014764:Viral Proteins / Q000494:pharmacology*
D006133:Growth Substances; D014764:Viral Proteins