Cloning and sequencing of the gene encoding benzaldehyde lyase from Pseudomonas fluorescens biovar I.
The gene (bzl) encoding benzaldehyde lyase (BL) from Pseudomonas fluorescens biovar I has been cloned and characterized. The nucleotide sequence contains an open reading frame encoding a protein of 563 amino acids. The deduced BL protein shares little homology with proteins contained in the NBRF-PIR data bank. However, the higher homologies (up to about 28%) were obtained with enzymes that also utilize thiamine pyrophosphate as a cofactor.
Laboratorio de Bioquímica, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago.
P
P
Hinrichsen
I
I
Gómez
R
R
Vicuña
D000446:Aldehyde-Lyases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000235:genetics
D004269:DNA, Bacterial; D000446:Aldehyde-Lyases; C059416:benzaldehyde lyase