Th1 and Th2 CD4+ T cell clones specific for Plasmodium chabaudi but not for an unrelated antigen protect against blood stage P. chabaudi infection.
The host protective immune response to blood stage malaria infection was studied using Plasmodium chabaudi chabaudi (P.chabaudi) in NIH mice. It has been shown previously that CD4+ cells are critically required for protection against erythrocytic infection. Mice lacking a functional CD4+ cell compartment suffer unremitting patent primary parasitemias for at least 60 days after infection. Here, we report that the adoptive transfer of eight P. chabaudi-specific CD4+ T cell clones of either the Th1 or Th2 type to mice rendered CD4-depleted by adult thymectomy and anti-CD4 monoclonal antibody therapy fully restored the ability of recipients to control challenge infection. Control Th1 and Th2 clones specific for an unrelated antigen, ovalbumin, were unable to confer a comparable level of protection in CD4-depleted mice, even though they received regular doses of the antigen. These data demonstrate that protective immunity to asexual P. chabaudi parasites can be mediated through immune CD4+ T cell clones of either the Th1 or the Th2 subset.
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Scotland.
A W
AW
Taylor-Robinson
R S
RS
Phillips
D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D005434:Flow Cytometry; D008213:Lymphocyte Activation; D008222:Lymphokines; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000276:immunology; D016788:Plasmodium chabaudi / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000637:transplantation; D013934:Thymectomy; D013950:Thymus Gland / Q000502:physiology
D000911:Antibodies, Monoclonal; D008222:Lymphokines; D010047:Ovalbumin