Modified seroneutralization assay for measles virus antibody detection.
We describe a simple, inexpensive, sensitive and specific modified seroneutralization assay for use on a wide scale to screen for the presence of measles antibodies. After only one reaction (1 h) and a 3-day incubation period at 37 degrees C, the test can be easily read using a rapid, inexpensive methylene blue/phenol staining procedure. The determination of protective antibody titres by modified and standard seroneutralization methods was strongly correlated (corr. coff. = 0.98).
Instituto de Virología, Dr. J.M. Vanella, Facultad de Cencia Médicas, Unversidad Nacional de Córdoba, Ciudad Universitaria, Argentina.
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SV
Nates
G Y
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Rey
M O
MO
Giordano
M T
MT
Zapata
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A
Depetris
J
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Boshell
D000914:Antibodies, Viral / Q000097:blood*; D006801:Humans; D008459:Measles virus / Q000276:immunology*; D009500:Neutralization Tests / Q000379:methods*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral