Chemotaxis, sporulation, and larvicide production in Bacillus sphaericus 2362. The influence of L-ethionine, and of aminophenylboronic acid.
4-Aminophenylboronic acid (APBA), a known inhibitor of sporulation in Bacilli, as well as L-ethionine, a known inhibitor of chemotaxis in Enterobacteria, inhibited both sporulation and chemotactic behavior but not growth of Bacillus sphaericus. Both compounds also inhibited the methyl group turnover on the methyl-accepting chemotaxis protein (P53) in this microorganism. Sporulation of B. sphaericus was inhibited only when APBA was added to the growing culture before the late logarithmic stage. It was previously demonstrated that the ability of B. sphaericus to respond to chemoattractants sharply declines at the same age of the culture. Thus, it seems plausible that the action of both inhibitors upon sporulation may be attributed to the inhibition of some regulatory pathway common to chemotaxis and sporulation and involving protein methylation. Possible exchange of the nutrient depletion-related sensory information between chemotaxis and sporulation systems at the level of methyl group transfer is discussed.
Andreev
J
J
Department of Biological Chemistry, A. Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel.
Dibrov
P A
PA
Klein
D
D
Braun
S
S
D001407:Bacillus / Q000187:drug effects / Q000502:physiology*; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001897:Boronic Acids / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects; D005001:Ethionine / Q000494:pharmacology*; D008565:Membrane Proteins / Q000037:antagonists & inhibitors; D008715:Methionine / Q000378:metabolism; D000072236:Methyl-Accepting Chemotaxis Proteins; D008745:Methylation; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001897:Boronic Acids; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; C046134:binB protein, Bacillus sphaericus; C028592:3-aminobenzeneboronic acid; D008715:Methionine; D005001:Ethionine