Real time measurement of gene expression in living endocrine cells.
The mechanisms underlying hormonal gene expression are complex, and key regulatory events are often temporally separated. Our understanding of these dynamic processes has been hindered in part by the lack of a method suitable for making dynamic measurements of gene expression. In an attempt to develop such a system, we modified conventional luciferase reporter technology to enable real time measurement of gene expression in living endocrine cells.
Frawley
L S
LS
Dept. of Cell Biology and Anatomy, Medical University of South Carolina, Charleston 29425.
Faught
W J
WJ
Nicholson
J
J
Moomaw
B
B
D000818:Animals; D003199:Computer Systems; D004702:Endocrine Glands / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D049454:Firefly Luciferin; D015870:Gene Expression; D017930:Genes, Reporter; D005821:Genetic Techniques; D008027:Light; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D010781:Photography; D051381:Rats
D049454:Firefly Luciferin; D008156:Luciferases