Identification of the aromatase in the breast carcinoma cell lines T47D and MCF-7.
The presence of estrogens in tumour cells is considered to be a critical factor for the development of the hormone-dependent forms of breast cancer. The last, rate-limiting step of estrogen biosynthesis is controlled by cytochrome P-450 type enzyme complex named aromatase. In the present study we determined and characterized the expression of aromatase mRNA in the breast carcinoma cell lines T47D and MCF-7. The expression was characterized by slot blot hybridization, reverse transcriptase-polymerase chain reaction technique and Northern hybridization analysis. Northern blotting revealed the presence of 4.4 kb and 2.4 kb messengers in both cell lines.
Sadekova
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Department of Oncology, Montreal General Hospital, McGill University, Que., Canada.
Tan
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Chow
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D001141:Aromatase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000201:enzymology*; D002460:Cell Line; D005260:Female; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010920:Placenta / Q000201:enzymology; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D001141:Aromatase