Molecular cloning and tissue distribution of a putative member of the rat opioid receptor gene family that is not a mu, delta or kappa opioid receptor type.
A novel G protein-coupled receptor was cloned by PCR and homology screening. Its deduced amino acid sequence is 47% identical overall to the mu, delta and kappa opioid receptors and 64% identical in the putative transmembrane domains. When transiently expressed in COS-7 cells this receptor did not bind any of the typical mu, delta or kappa opioid receptor ligands with high affinity. In situ hybridization analysis revealed that LC132 mRNA is highly expressed in several rat brain areas, including the cerebral cortex, thalamus, subfornical organ, habenula, hypothalamus, central gray, dorsal raphe, locus coeruleus and the dorsal horn of the spinal cord. Based on this distribution and its high homology with the mu, delta and kappa opioid receptors, it is proposed that LC132 is a new member of the opioid receptor family that is involved in analgesia and the perception of pain.
Vollum Institute for Advanced Biomedical Research, OHSU, Portland, OR 97201.
J R
JR
Bunzow
C
C
Saez
M
M
Mortrud
C
C
Bouvier
J T
JT
Williams
M
M
Low
D K
DK
Grandy
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D002460:Cell Line; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000502:physiology; D015870:Gene Expression; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011957:Receptors, Opioid / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017465:Receptors, Opioid, delta / Q000737:chemistry; D017473:Receptors, Opioid, kappa / Q000737:chemistry; D017450:Receptors, Opioid, mu / Q000737:chemistry; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D014018:Tissue Distribution
D012333:RNA, Messenger; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins