[Test-systems of polymerase chain reaction for determining the tuberculosis pathogen].
Two polymerase chain reaction-based test systems were used to identify Mycobacteria tuberculosis by using a clinical material for practical health purposes. The former test system strictly specifically enables M. tuberculosis to be detected. The latter also allows one to differentiate a BCG vaccine strain from the M. tuberculosis. The test systems were tested by using 11 clinical sputum samples, three of them were taken from patients with fibrocavernous tuberculosis, two from those with focal tuberculosis and one from that with infiltrative one, 5 from those with non-specific pulmonary diseases (chronic bronchitis and asthma). In addition, one serum sample from a patient with fibrocavernous tuberculosis was examined. All tests obtained from patients with tuberculosis were positive, whereas those from patients with non-specific pulmonary diseases were negative. This suggests that differentiation of BCG vaccine strain from M. tuberculosis can be used both in model experiments and in the study of clinical material lysates.
Nesterenko
L N
LN
Aksënov
M Iu
MIu
Grishina
T D
TD
Shaginian
I A
IA
Golyshevskaia
V I
VI
Khomenko
A G
AG
Gintsburg
A L
AL
Prozorovskiĭ
S V
SV
D001500:BCG Vaccine / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D001769:Blood / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology*
D001500:BCG Vaccine; D004279:DNA, Viral
Test-sistemy na osnove polimeraznoĭ tsepnoĭ reaktsii dla identifikatsii vozbuditelia tuberkulëza.