Effect of sodium butyrate treatment on the granule morphology, histamine level and elemental content of the bone marrow-derived mast cell.
Mast cells derived from the bone marrow of BALB/mice (BMMC) were cultured and their growth ceased with sodium butyrate. Sodium butyrate treatment (1mM, 4 days) caused maturation of the granules, an increased histamine content from approx. 1 pg/cell to 4 pg/cell. X-ray microanalysis revealed that maturation of the granules was accompanied by the increase in relative weight percent of sodium, phosphorus and sulphur, with concomitant decrease in chloride. The sulphur to potassium ratio increased three-fold in butyrate-treated mast cells. The existence of a different elemental composition during mast cell maturation may provide additional parameter for rapid discrimination of mast cell subpopulations.
Department of Toxicity Evaluation, Institute of Occupational Medicine, Lódź, Poland.
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Dalen
D000818:Animals; D001854:Bone Marrow Cells; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002478:Cells, Cultured; D002712:Chlorides / Q000032:analysis; D003594:Cytoplasmic Granules / Q000737:chemistry* / Q000648:ultrastructure*; D004577:Electron Probe Microanalysis; D006632:Histamine / Q000032:analysis*; D066298:In Vitro Techniques; D008407:Mast Cells / Q000737:chemistry* / Q000166:cytology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D010758:Phosphorus / Q000032:analysis*; D011188:Potassium / Q000032:analysis; D012964:Sodium / Q000032:analysis*; D013455:Sulfur / Q000032:analysis
D002087:Butyrates; D002712:Chlorides; D020148:Butyric Acid; D010758:Phosphorus; D013455:Sulfur; D006632:Histamine; D012964:Sodium; D011188:Potassium