In vitro metabolism of 3,3',4,4'-tetrachlorobiphenyl in relation to ethoxyresorufin-O-deethylase activity in liver microsomes of some wildlife species and rat.
A qualitative study was performed of the capacity of hepatic microsomes of several wildlife species to metabolize 3,3',4,4'-tetrachlorobiphenyl (TCB). Hepatic microsomes of species environmentally exposed to polychlorinated biphenyls (PCBs): harbour porpoise (Phocoena phocoena), harbour seal (Phoca vitulina), common tern (Sterna hirundo), and hepatic microsomes from species experimentally exposed to PCBs: eider duck (Somateria mollissima), rainbow trout (Salmo gairdneri), flounder (Platichthys flesus), and Wistar rat, were incubated with 14C-labelled TCB ([14C]TCB). The mammals and birds were able to metabolize TCB at a rate that correlated with their ethoxyresorufin-O-deethylase (EROD) activity. No [14C]TCB metabolism was observed in the fish, despite elevated EROD activity in the trout. HPLC analysis of diisopropylether extracts of the microsomal incubations indicated the presence of 4-OH-, 5-OH-, and 6-OH-tetrachlorobiphenyl metabolites and a yet unidentified metabolite. The ratio of the different hydroxy metabolites formed varied for the various species.
Murk
A
A
Department of Toxicology, Agricultural University, Wageningen, Netherlands.
Morse
D
D
Boon
J
J
Brouwer
A
A
D000818:Animals; D001717:Birds / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004289:Dolphins / Q000378:metabolism; D004372:Ducks / Q000378:metabolism; D005399:Fishes / Q000378:metabolism; D006900:Hydroxylation; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012474:Salmon / Q000378:metabolism; D046048:Seals, Earless / Q000378:metabolism; D013045:Species Specificity
D003577:Cytochrome P-450 Enzyme System; D011078:Polychlorinated Biphenyls; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1; C028451:3,4,3',4'-tetrachlorobiphenyl