Effects of oxygen, pH and nitrate concentration on denitrification by Pseudomonas species.
The production of nitrogen-containing gases by denitrification in three organisms was examined using membrane inlet mass spectrometry. The effects of O2 (during both growth and maintenance) and of pH, nitrate concentration and carbon source were tested in non-proliferating cell suspensions. Two strains of Pseudomonas aeruginosa were capable of co-respiration of NO3- and O2 and, under controlled O2 supply, gave oscillatory denitrification. Variations in culture and assay conditions affected both the rate of denitrification and the ratio of end products (N2O:N2). Higher rates were seen following anaerobic growth. Optimum values of pH and nitrate concentration for denitrification are given. Generally, the optimum pH was 7.0-7.5, approximately that of the growth medium. Optimum nitrate concentration was generally 20 mM.
Microbiology Group, School of Pure and Applied Biology, University of Wales College of Cardiff, UK.
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Thomas
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Lloyd
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Boddy
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000332:Aerobiosis; D000693:Anaerobiosis; D002245:Carbon Dioxide / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods; D009566:Nitrates / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D017680:Potassium Compounds / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000254:growth & development / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
D000085:Acetates; D009566:Nitrates; D017680:Potassium Compounds; D013386:Succinates; D002245:Carbon Dioxide; D019802:Succinic Acid; D005947:Glucose; D009609:Nitrous Oxide; D009584:Nitrogen; D019342:Acetic Acid; C023844:potassium nitrate