Nucleotide sequence and distribution of the pepPN gene from Lactobacillus helveticus CNRZ32.
The Lactobacillus helveticus CNRZ32 gene encoding a di-/tri- pepidase with prolinase activity (pepPN) was sequenced. An open reading frame of 912 base pairs was identified corresponding to a peptide with a molecular mass of 35.04 kDa. Southern hybridization indicated that the gene sequence is well conserved in strains of lactobacilli and pediococci.
Dudley
E G
EG
Department of Food Science, University of Wisconsin-Madison 53706.
Steele
J L
JL
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004150:Dipeptidases / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial; D007778:Lactobacillus / Q000235:genetics*; D008969:Molecular Sequence Data
D004150:Dipeptidases; C022454:prolyl dipeptidase