[Detection of RB gene in osteosarcoma by PCR].
We have established a stable PCR system and studied the structural anomalies of RB gene by it in osteosarcoma, previous studies have proved that 13-17 exons of RB gene are the deletion hotpoints. We designed a pair of perimers, RB1, RB2 stretching across 14-16 exons and studied 10 cases of osteosarcoma. No structural anomaly was found in the leucocyte. Two cases (20%) showed no PCR product. This result demonstrates for the first time the deletion of RB gene is present in Chinese osteosarcoma patients.
Beijing Medical University.
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HG
Li
D001483:Base Sequence; D001859:Bone Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D017353:Gene Deletion; D016161:Genes, Retinoblastoma; D006801:Humans; D008969:Molecular Sequence Data; D012516:Osteosarcoma / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004273:DNA, Neoplasm