Immunocytochemical analysis of band 3 protein in renal cell carcinoma, nephroblastoma, and oncocytoma.
Band 3 protein is an anion-exchange protein that in the human kidney is restricted to a subpopulation of collecting duct-intercalated cells; however, it is absent in the metanephric-derived cells of the nephron. We have studied band 3 protein expression in a series of 60 renal tumors that included 10 oncocytomas, 42 renal cell carcinomas, and eight nephroblastomas by using frozen tumors and a monoclonal antibody. We detected band 3 protein expression in nine of 10 oncocytomas but not in any renal cell carcinomas or nephroblastomas. This finding confirms that band 3 protein is a specific marker for oncocytoma and therefore may have a potential diagnostic application. It also supports the contention that renal cell carcinoma and oncocytoma are histogenically unrelated; the former is metanephric blastema derived and the latter is ampullary bud derived.
Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City 52242.
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D018249:Adenoma, Oxyphilic / Q000737:chemistry*; D000328:Adult; D001457:Anion Exchange Protein 1, Erythrocyte / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000737:chemistry*; D002648:Child; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000737:chemistry*; D009396:Wilms Tumor / Q000737:chemistry*
D001457:Anion Exchange Protein 1, Erythrocyte