Effect of ascorbic acid on the binding of cloxacillin sodium to bovine serum albumin.
Using dynamic and equilibrium dialysis methods, it has been demonstrated that ascorbic acid (CAS 50-81-7) inhibits the binding of cloxacillin sodium (CAS 7081-44-9) to bovine serum albumin (BSA) in vitro. Normally, ascorbic acid has a lower number of binding sites and a much lower binding constant for BSA than cloxacillin sodium. There is an indication that ascorbic acid inhibits the binding of cloxacillin to BSA through a noncompetitive mechanism. The probable interactions leading to the non-competitive inhibition were suggested.
Department of Pharmaceutics, University of Nigeria, Nsukka.
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Okore
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002417:Cattle; D003023:Cloxacillin / Q000097:blood*; D003864:Depression, Chemical; D003956:Dialysis; D011485:Protein Binding / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000378:metabolism*
D012710:Serum Albumin, Bovine; D003023:Cloxacillin; D001205:Ascorbic Acid