A common deletion in two gamma ray induced rat pulmonary tumor cell lines.
Subtraction hybridization was performed on normal WAG/Rij rat DNA with DNA from a syngeneic Ir-192 induced pulmonary tumor cell line L37. The residual DNA was amplified by means of sequence-independent PCR. This procedure yielded a sequence, of which multiple copies are present in normal rat DNA. In the tumor line L37 two restriction fragments hybridizing with this repeat sequence are lacking. In another Ir-192 induced pulmonary tumor line, L33, one of these fragments was also lacking. This indicates a common deletion in the two tumor lines.
Department of Experimental Dosimetry, TNO, Rijswijk, The Netherlands.
P
P
Van Klaveren
J
J
De Bruijne
H
H
Van der Winden
H B
HB
Kal
P
P
Bentvelzen
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000032:analysis*; D005720:Gamma Rays; D007496:Iridium Radioisotopes; D008175:Lung Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D011914:Rats, Inbred BN; D011919:Rats, Inbred Strains; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012091:Repetitive Sequences, Nucleic Acid; D017384:Sequence Deletion; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D004273:DNA, Neoplasm; D007496:Iridium Radioisotopes; D004247:DNA