Polymorphism of tumour necrosis factor-alpha (TNF-alpha) at position -308 in relation to ankylosing spondylitis.
In addition to HLA-B27, other genetic factors are thought to be involved in the pathogenesis of ankylosing spondylitis (AS). Because of the localization, in the proximity of the HLA-B locus, and the biological activities of TNF-alpha, we investigated the association between AS and a single base polymorphism located at position -308 of the TNF-alpha gene. An allele-specific polymerase chain reaction was developed to monitor this polymorphism. The frequency of the TNF-alpha alleles was determined in 66 AS patients and 37 healthy controls. The TNF-alpha allele frequency was not significantly different between AS patients and controls.
Department of Ophthalmo-Immunology, Netherlands Ophthalmic Research Institute, Amsterdam.
G M
GM
Verjans
B M
BM
Brinkman
C E
CE
Van Doornik
A
A
Kijlstra
C L
CL
Verweij
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005787:Gene Frequency; D015796:HLA-B27 Antigen / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D013167:Spondylitis, Ankylosing / Q000235:genetics* / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D017931:DNA Primers; D015796:HLA-B27 Antigen; D014409:Tumor Necrosis Factor-alpha