Insulin receptor autophosphorylation sites tyrosines 1162 and 1163 control both insulin-dependent and insulin-independent receptor internalization pathways.
We previously reported that Chinese hamster ovary (CHO) cell lines overexpressing mutated human insulin receptors (hIRs) in which the tyrosine residues 1162 and 1163 were replaced by phenylalanines (CHO-Y2) exhibited a marked defect in hormone-induced receptor internalization as compared to CHO transfectants overexpressing wild-type hIRs (CHO-R). These two cell lines are now used to compare the role of tyrosines 1162-1163 in basal and ligand-stimulated receptor internalization as well as in receptor turnover. We show here that (1) in CHO-Y2 cells, basal endocytosis, like insulin-induced internalization, was markedly altered despite normal receptor turnover and (2) in both CHO-R and CHO-Y2 cells, basal receptor endocytosis was altered by tunicamycin, an inhibitor of protein N-glycosylation, whereas insulin-induced internalization was not. These results support a role for tyrosines 1162-1163 of the IR beta-subunit major autophosphorylation domain in both basal and ligand-stimulated receptor endocytosis and provide evidence that the two processes follow distinct pathways.
Reynet
C
C
Laboratoire de Biochimie et Biologie Cellulaire, I.N.S.E.R.M. U.181, Faculté de Médecine Saint-Antoine, Paris, France.
Caron
M
M
Magré
J
J
Picard
J
J
Cherqui
G
G
Capeau
J
J
D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D007328:Insulin / Q000494:pharmacology; D008024:Ligands; D010766:Phosphorylation; D011972:Receptor, Insulin / Q000187:drug effects / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism*
D007328:Insulin; D008024:Ligands; D014415:Tunicamycin; D014443:Tyrosine; D011972:Receptor, Insulin