Pseudomonas acidovorans: a bacterium capable of mineralizing 2-chloroaniline.
Prolonged adaptation of Ca-alginate immobilized cells of Pseudomonas acidovorans CA28 to a mixture of 3-chloroaniline (3-CA)1) and 2-CA and subsequently to 2-CA as sole substrate led to the isolation of another strain, termed CA50 with the additional capability of utilizing 2-CA as sole source of carbon, nitrogen, and energy. Batch-degradation of 190 mg/l of 2-CA at pH 6.1 by this newly isolated strain was achieved within 3 days, at higher concentrations up to 0.6 g/l increasing lag-phases and degradation periods were observed. Except chloride and ammonium no further metabolites were detectable in the medium. Mineralization of 2-CA proceeds via the modified ortho-cleavage pathway as demonstrated by the presence of catechol 1,2-dioxygenase (C120) activity, which is characterized by its substrate specificity and elution behaviour on DEAE-cellulose.
Abteilung für Umweltmikrobiologie, Technische Universität Wien, Osterreich.
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D000814:Aniline Compounds / Q000378:metabolism*; D001673:Biodegradation, Environmental; D050548:Catechol 1,2-Dioxygenase; D003470:Culture Media; D049308:Dioxygenases; D010105:Oxygenases / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D013379:Substrate Specificity
D000814:Aniline Compounds; D003470:Culture Media; C031392:3-chloroaniline; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; C025257:2-chloroaniline