Extraocular application of mitomycin C in a rabbit model: cytotoxic effects on the ciliary body and epithelium.
Since prolonged postoperative hypotony has been a frequent complication of glaucomatous filtration surgery in which mitomycin C (MMC) was used, it is important to determine the intraocular toxic effects of the drug. We placed MMC, in concentrations of 0.05, 0.2, and 1.0 mg/mL on the intact sclera of rabbits and obtained the eyes after 1, 7, and 28 days. Examination of the ciliary epithelium by transmission electron microscopy revealed an electron-dense material near the endoplasmic reticulum. Large intracellular vacuoles were present, and the mitochondria appeared swollen. These findings suggest a toxic effect of MMC on the ciliary epithelium in rabbits after extraocular application, possibly decreasing aqueous production.
Department of Ophthalmology, University of Cologne, Germany.
H
H
Mietz
K
K
Addicks
M
M
Diestelhorst
G K
GK
Krieglstein
D000818:Animals; D002924:Ciliary Body / Q000187:drug effects* / Q000473:pathology; D005260:Female; D008928:Mitochondria / Q000187:drug effects / Q000473:pathology; D016685:Mitomycin / Q000008:administration & dosage / Q000633:toxicity*; D010857:Pigment Epithelium of Eye / Q000187:drug effects* / Q000473:pathology; D011817:Rabbits; D012590:Sclera / Q000187:drug effects* / Q000473:pathology; D014617:Vacuoles / Q000187:drug effects / Q000473:pathology
D016685:Mitomycin