Transcription-modulatory activity of full-length E6 and E6*I proteins of human papillomavirus type 16.
The E6 gene of human papillomavirus type 16 (HPV16) has the potential to encode full-length as well as truncated E6 proteins (E6*I and E6*II) by alternative splicings. Spliced ORF E6*I is considered to facilitate the translation of the neighboring E7 ORF; however, the putative E6*I protein is suspected to be functionless. In this study, the transcription-modulatory functions of full-length E6 and E6*I proteins were examined using cDNAs from a cervical carcinoma cell line. E6*I cDNA was able to trans-activate the autologous P97 promoter and the heterologous adenovirus E2 promoter. Full-length E6 was found to trans-activate the heterologous promoter, but repress transcription from the autologous P97 promoter. The transcription-modulatory functions of full-length E6 and E6*I proteins suggested that transcriptional regulation of HPVs associated with mucosal malignant lesions is complex.
Department of Microbiology, School of Medicine, Chiba University, Japan.
H
H
Shirasawa
M H
MH
Jin
K
K
Shimizu
N
N
Akutsu
Y
Y
Shino
B
B
Simizu
D017502:Adenovirus E2 Proteins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002461:Cell Line, Transformed; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D000882:Haplorhini; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D027383:Papillomaviridae / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012097:Repressor Proteins / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*; D015533:Transcriptional Activation / Q000502:physiology
D017502:Adenovirus E2 Proteins; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins