Nucleotide sequence of an Onchocerca volvulus cDNA clone encoding an antigen homologous with the intermediate filament protein family.
A series of clones were isolated from an O. volvulus lambda gt11 cDNA library on the basis of strong recognition by sera from both Onchocerca volvulus or Wuchereria bancrofti patients. Several of these clones encoded a protein which clearly belonged to the intermediate filament (IF) class, and had most similarity with A1IF-A from Ascaris lumbricoides and a number of IF proteins from molluscs. Western blotting experiments implied that the first coiled domain appears to be markedly less antigenic in vivo than the remainder of the O. volvulus protein.
Department of Molecular Sciences, James Cook University of North Queensland, Townsville, Australia.
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D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007381:Intermediate Filament Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D017181:Onchocerca volvulus / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000947:Antigens, Helminth; D007106:Immune Sera; D007381:Intermediate Filament Proteins; C088304:intermediate filament protein 1, Onchocerca volvulus; D004247:DNA