Physical mapping of chromosome 17 cosmids by fluorescence in situ hybridization and digital image analysis.
We used fluorescence in situ hybridization and digital image analysis to localize cosmids along human chromosome 17. Seventy-one cosmids were selected at random from a chromosome 17 library constructed from a partial Sau3AI digest of flow-sorted chromosomes from a mouse-human hybrid cell line. Sixty-three of these (89%) gave a signal only on chromosome 17. The 40 cosmids producing the most distinct hybridization signals in metaphase and interphase cells were precisely mapped using digital image analysis. An additional 20 cosmids, previously mapped by linkage analysis, were also mapped. The order of these probes determined by metaphase mapping was consistent with the order determined by linkage analysis.
University of California, San Francisco.
O P
OP
Kallioniemi
A
A
Kallioniemi
L
L
Mascio
D
D
Sudar
D
D
Pinkel
L
L
Deaven
J
J
Gray
D002874:Chromosome Mapping / Q000379:methods*; D002886:Chromosomes, Human, Pair 17 / Q000648:ultrastructure; D003360:Cosmids / Q000235:genetics*; D015342:DNA Probes; D015723:Gene Library; D008040:Genetic Linkage; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000235:genetics; D008677:Metaphase / Q000235:genetics
D015342:DNA Probes