Reduction of sunburn damage to skin by topical application of vitamin E acetate following exposure to ultraviolet B radiation: effect of delaying application or of reducing concentration of vitamin E acetate applied.
The skin of the skh-1 mouse after ultraviolet B (280-320 nm, UVB) irradiation shows the pathological changes typical of sunburn damage: spongiosis (edematous spaces) around some cells, necrosis of keratinocytes, giving rise to sunburn cells, inflammatory infiltration of polymorphonuclear leucocytes, etc. In our previous study, these were accompanied by erythema, increased skin sensitivity, and edematous swelling. The topical application of tocopherol acetate (TA) immediately after the UVB exposure decreased these changes. In this paper, multiple measurements of the skin thickness were made at different locations along the magnetic resonance imaging (MRI) cross-sectional image of the skin. This permits effects to be quantified with (if desired) the contralateral half of the back serving as an internal control, either exposed (positive control) or unexposed (negative control). Topical application of TA resulted in an increase in the concentration of free tocopherol in the skin. No qualitative differences in ultrastructural appearance of the UVB-irradiated, TA-treated skin could be discerned by careful examination. In vivo high resolution video microscopy of blood flow in venules of the irradiated mouse ear revealed a large (tenfold) but not statistically significant decrease in stationary lymphocytes adhering to the venule walls. The delaying of the application of TA up to 8 hours after the termination of UVB irradiation still offered statistically significant protection as did immediate application of 5% TA in diluent Myritol 318 (Delios S, Henkel).
Department of Biochemistry, Faculty of Medicine, University of Western Ontario, London, Canada.
J R
JR
Trevithick
D T
DT
Shum
S
S
Redae
K P
KP
Mitton
C
C
Norley
S J
SJ
Karlik
A C
AC
Groom
E E
EE
Schmidt
D000287:Administration, Topical; D000818:Animals; D004305:Dose-Response Relationship, Drug; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008854:Microscopy, Electron; D011833:Radiation Injuries, Experimental / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control; D011837:Radiation-Protective Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D012867:Skin / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects* / Q000648:ultrastructure; D012879:Skin Physiological Phenomena; D013471:Sunburn / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors; D024505:Tocopherols; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D011837:Radiation-Protective Agents; D014810:Vitamin E; D024502:alpha-Tocopherol; D024505:Tocopherols