Identification of a new member of the protegrin family by cDNA cloning.
The porcine leukocyte protegrins are a family of cysteine-rich antimicrobial peptides the primary structures of which combine features of defensins and tachyplesins. We cloned three protegrins from porcine bone marrow mRNA by PCR, including one (PG-4) that was previously unknown. The 691 bp protegrin cDNAs were > 98.8% identical, and each was surrounded by highly conserved 5' and (in some instances) 3' sequences present in structurally dissimilar antimicrobial and LPS-binding peptides of animal leukocytes.
Department of Medicine, UCLA Center for the Health Sciences 90024.
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Zhao
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Liu
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Lehrer
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D001853:Bone Marrow / Q000737:chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017385:Sequence Homology; D013552:Swine
D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; C084515:cathelin; C087841:protegrin-4