Skin-window-induced inflammation in breast cancer patients: a study of macrophage migration.
A decreased number of macrophages has previously been demonstrated by means of skin-window assays in operable breast cancer patients. However, in this type of cellular inflammatory response, a varying intensity of cellular activation, cellular recruitment and macrophage chemotaxis exists. In this study, we performed skin windows after stimulation by a chemo-attractant (FMLP) and a recall antigen (Candidin-latex). Cellular responses were classified in 19 breast cancer patients according to the presence (A+) or absence (A0) of macrophage activation by comparison to healthy controls. In 13 A0 patients, the admixture of a cytokine (IFN alpha) and an immunomodulator (P40) to one agent or the other or both resulted in the restoration of macrophage functions. Our study shows that uniform cellular responses to chemo-attractants and antigens are observed in healthy and not immunocompromised individuals as assessed by skin-window tests. However, the cellular response recorded in immunodeficient cancer patients is altered. It also shows that the admixture to chemoattractants and antigens of particular cytokines or better still of an immunomodulator displaying a wide spectrum of activity offers a way of restoring macrophage functions. Individual responses to immunotherapy in clinical oncology may be related to such results.
Hopital des Diaconesses, service de chirurgie générale et gynécologique, Paris.
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Henocq
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Villet
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Bizzini
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D001943:Breast Neoplasms / Q000276:immunology*; D002464:Cell Migration Inhibition; D003872:Dermatitis / Q000276:immunology*; D005260:Female; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology; D018942:Macrolides; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D011994:Recombinant Proteins; D012884:Skin Window Technique
D000276:Adjuvants, Immunologic; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D018942:Macrolides; D011994:Recombinant Proteins; C010428:candidin; D009240:N-Formylmethionine Leucyl-Phenylalanine