Rapid determination of antifungal activity by flow cytometry.
We have developed a rapid assay of antifungal activity which utilizes flow cytometry to detect accumulation of a vital dye in drug-damaged fungal cells. Results of these studies suggest that flow cytometry may provide an improved, rapid method for determining and comparing the antifungal activities of compounds with differing modes of action.
Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, Indiana 46202.
L
L
Green
B
B
Petersen
L
L
Steimel
P
P
Haeber
W
W
Current
D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D015169:Colony Count, Microbial; D003455:Cryptococcus neoformans / Q000187:drug effects; D054714:Echinocandins; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000379:methods*; D015725:Fluconazole / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects* / Q000378:metabolism; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010456:Peptides, Cyclic / Q000494:pharmacology; D011419:Propidium / Q000493:pharmacokinetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013997:Time Factors
D000935:Antifungal Agents; D054714:Echinocandins; D010456:Peptides, Cyclic; D011419:Propidium; D015725:Fluconazole; C042101:cilofungin