A flow cytometric assay reveals an enhancement of phagocytosis by platelet activating factor in murine peritoneal macrophages.
Phagocytosis of fluorescein isothiocyanate-labeled latex beads by peritoneal macrophages from thioglycollate-stimulated mice was examined in vitro by means of flow cytometry. This assay revealed that platelet activating factor (PAF), a known inflammatory mediator, enhanced the phagocytosis in a dose-dependent manner. The enhancement was completely suppressed by a PAF antagonist, Y-24180, and partly suppressed by another antagonist, CV-3988. The phagocytosis of both nonstimulated and PAF-stimulated macrophages was suppressed in Ca(2+)-free ethylene glycol bis(beta-aminoethyl ether) N,N'-tetraacetic acid-containing solution, but the phagocytosis was enhanced by PAF even in this solution. These results suggest that phagocytosis by macrophages is enhanced by PAF in allergic and inflammatory reactions.
Department of Physiology, Shimane Medical University, Izumo, Japan.
M
M
Ichinose
N
N
Hara
M
M
Sawada
T
T
Maeno
D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D066298:In Vitro Techniques; D007840:Latex; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects* / Q000502:physiology; D010742:Phospholipid Ethers / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology* / Q000502:physiology; D014230:Triazoles / Q000494:pharmacology
D001381:Azepines; D007840:Latex; D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; D014230:Triazoles; C068035:4-(2-chlorophenyl)-2-(2-(4-isobutylphenyl)ethyl)-6,9-dimethyl-6H-thieno(3,2-f)(1,2,4)triazolo(4,3-a)(1,4)diazepine; C037913:CV 3988; D002118:Calcium