Fractured carbon fiber-based biosensor for glucose.
A microsensor for glucose was constructed using DuPont E120 fibers, which exhibit extensive fracturing upon severe anodic pretreatment. The fracturing is accompanied by an increase in the electrochemical surface area of several orders of magnitude, which is exploited here in the design of a microsensor. Following fracture, the fibers were platinized to enhance their ability to detect enzymatically produced hydrogen peroxide, and glucose oxidase was immobilized at the surface of the fiber in polypyrrole. When operated amperometrically in flow injection analysis, the treated electrodes showed a linear response to injected glucose concentration up to 10 mM, with an observed Km' near 20 mM. The sensors were found to be stable for up to 2 months when stored dry at 4 degrees C.
Department of Chemistry, Lake Forest College, Illinois 60045.
J W
JW
Furbee
T
T
Kuwana
R S
RS
Kelly
D015374:Biosensing Techniques; D002244:Carbon; D004800:Enzymes, Immobilized / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism
D004800:Enzymes, Immobilized; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose