title: Efficient identification and regional positioning of YAC and cosmid clones to human chromosome 21 by radiation fusion hybrids.
abstract: The use of integrated mapping strategies involving bacterial, yeast, and rodent cells as hosts simplifies the construction of maps, which combine long-range order, high resolution, and easy access to the cloned DNA. Radiation-fusion hybrids offer a specially powerful long-range mapping system for human chromosomes. We describe here techniques for establishing a radiation-fusion hybrid map of Chromosome (Chr) 21q and its integration with local information on YAC and cosmid positions.
authors.affiliation: Department of Genome Analysis, Imperial Cancer Research Fund, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Kumlien
authors.forename: T
authors.initials: T
authors.lastname: Labella
authors.forename: G
authors.initials: G
authors.lastname: Zehetner
authors.forename: R
authors.initials: R
authors.lastname: Vatcheva
authors.forename: D
authors.initials: D
authors.lastname: Nizetic
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast; D002891:Chromosomes, Human, Pair 21; D006224:Cricetinae; D004926:Escherichia coli; D005819:Genetic Markers; D005821:Genetic Techniques; D005822:Genetic Vectors; D005838:Genotype; D006801:Humans; D006822:Hybrid Cells
chemical_list: D005819:Genetic Markers