title: [Cell culture model and concept of bone surface].
abstract: Cellular differentiation areas leading to bone nodular formation from rat bone calvaria cells were studied under optic and electronic transmission microscope. 3H-thymidine labeling, BrdU proliferating cells and alkaline phosphatase cytoenzymatic reaction allowed us to dynamically describe the development of a cellular group called "Active Osteogenic Unit" (AOU) responsible for bone nodule formation. This AOU was formed by synchronized, localized and increased cell surface proliferation allowing a three dimensional cellular organization leading to an underneath osteoblastic cell proliferation. The osteocyte embedding process observed secondly are in relation with the cell heterogeneity forming the AOU. AOU's final cell activity might be a triggering factor in bone remodeling.
authors.affiliation: Laboratoire de Biologie-Odontologie, Institut Biomédical des Cordeliers, Paris, France.
authors.forename: J R
authors.initials: JR
authors.lastname: Nefussi
authors.forename: J M
authors.initials: JM
authors.lastname: Sautier
authors.forename: N
authors.initials: N
authors.lastname: Forest
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001842:Bone and Bones / Q000166:cytology* / Q000196:embryology / Q000201:enzymology; D001973:Bromodeoxyuridine; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D008854:Microscopy, Electron; D008953:Models, Anatomic; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013936:Thymidine
chemical_list: D000469:Alkaline Phosphatase; D001973:Bromodeoxyuridine; D013936:Thymidine
vernacular_title: Modèle de culture et concept de surface osseux.