title: Aphidicolin induces myogenic differentiation in the human rhabdomyosarcoma cell line KFR.
abstract: The effects of aphidicolin, a specific inhibitor of DNA polymerase alpha, on cell growth, DNA synthesis and myogenic differentiation in the human alveolar rhabdomyosarcoma cell line KFR were studied. The treatment with aphidicolin at 5 x 10(-6) M concentration, which completely inhibited DNA synthesis and cell growth, induced morphological differentiation of small mononuclear cells to elongated, multinucleated (myotube-like) structures. The morphological differentiation was accompanied by the expression of skeletal muscle myosin; about 30% myosin-positive cells were observed after 14 days of treatment, compared to 2.3% in untreated cultures. The results showed that aphidicolin induces differentiation of human rhabdomyosarcoma cells and that multinucleated myotube-like elements may develop simply by cell fusion without cell division and DNA synthesis.
authors.affiliation: Centre of Hygiene, J. W. Goethe-University, Frankfurt a. M., Fed. Rep. of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: P H
authors.initials: PH
authors.lastname: Driever
authors.forename: H
authors.initials: H
authors.lastname: Rabenau
authors.forename: M
authors.initials: M
authors.lastname: Novak
authors.forename: M
authors.initials: M
authors.lastname: Stefanik
authors.forename: B
authors.initials: B
authors.lastname: Kornhuber
authors.forename: H W
authors.initials: HW
authors.lastname: Doerr
mesh_terms: D016590:Aphidicolin / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D009132:Muscles / Q000166:cytology*; D009218:Myosins / Q000096:biosynthesis; D018232:Rhabdomyosarcoma, Alveolar / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
chemical_list: D016590:Aphidicolin; D009218:Myosins