title: Isolation of Serratia marcescens mutants which could overproduce and excrete Escherichia coli alkaline phosphatase and beta-lactamase.
abstract: Serratia marcescens mutants, which excrete Escherichia coli alkaline phosphatase (APase) encoded by the plasmid-bearing phoA gene, were isolated after mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine. These mutants produced two to four times as much APase as did the parent strain under a phosphate-limiting condition, and more than 70% of the enzyme was released into the culture medium. In addition, overproduction and excretion of beta-lactamase was observed in these mutants.
authors.affiliation: Department of Biotechnology, College of Agriculture, Ehime University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Asdornnithee
authors.forename: K
authors.initials: K
authors.lastname: Akiyama
authors.forename: R
authors.initials: R
authors.lastname: Takata
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D012706:Serratia marcescens / Q000201:enzymology* / Q000235:genetics; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000469:Alkaline Phosphatase; D001618:beta-Lactamases