title: [Binding and degradation of 125I insulin of erythrocyte receptors--effect of physical exertion].
abstract: The rate of binding and degradation of 125I insulin by red blood cell receptors was determined in children with insulin-dependent diabetes at rest and after physical exercise of moderate intensity (35% VO2max). Anthropometric values, duration of the disease, parameters of lipid and carbohydrate metabolism as well as blood serum levels of insulin and C-peptide were used as the features characterizing the studied children. The determination of the amount of bound and degraded 125I insulin by intact erythrocytes was carried out by the modified method of Gambhir. The method of Hildebrandt was used when the analogous measurements were carried out with hemolysate as a source of receptor. It was found that physical exercise of aerobic type resulted in an increase in the amount of 125I-insulin bound to the red blood cell receptors by about 30%. This value was not statistically significant. Statistically significant differences were, however, obtained for post-exercise concentrations of insulin and C-peptide in blood serum and for the amounts of 125I-insulin degraded by intact erythrocytes and by hemolysate. The observed increase in the amount of degraded insulin may reflect an increase in the activity of insulinase, an enzyme bound to the cell membrane and partly present in the cytoplasm.
authors.affiliation: Katedra Fizjologii, Biochemii i Higieny Akademii Wychowania Fizycznego, Poznaniu.
authors.forename: S
authors.initials: S
authors.lastname: Lucja
authors.forename: B
authors.initials: B
authors.lastname: Franciszek
authors.forename: R
authors.initials: R
authors.lastname: Tadeusz
authors.forename: W
authors.initials: W
authors.lastname: Mieczysław
authors.forename: G
authors.initials: G
authors.lastname: Jerzy
mesh_terms: D000293:Adolescent; D000886:Anthropometry; D002096:C-Peptide / Q000097:blood; D050260:Carbohydrate Metabolism; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000097:blood / Q000493:pharmacokinetics*; D007457:Iodine Radioisotopes; D005082:Physical Exertion / Q000502:physiology*; D011972:Receptor, Insulin / Q000378:metabolism*
chemical_list: D002096:C-Peptide; D007328:Insulin; D007457:Iodine Radioisotopes; D011972:Receptor, Insulin
vernacular_title: Wiazanie i degradacja insuliny 125I przez receptory erytrocytów--wpływ wysiłku fizycznego.