title: Comparison between immunofluorescence and enzyme-linked-immunosorbent assay in the determination of serum HHV-6 IgG.
abstract: 546 human sera from healthy subjects, subjects with cytomegalovirus or Epstein-Barr virus infection and from cordal blood were tested for the human herpesvirus-6 (HHV-6) IgG content in an enzyme-linked immunosorbent assay (ELISA) to verify results previously obtained with an immunofluorescence assay (IFA). The HHV-6 isolate CV, obtained from a child with exanthem subitum, employed as antigen in both tests, belonged to HHV-6 variant B. The ELISA results were similar to those obtained with IFA. 445/546 (81.5%) sera had the same type of reactivity with the two serological procedures. To establish whether different HHV-6 strains may give different serological results, 117 out of the 546 sera tested with IFA against CV infected cells were also tested against cells infected with the HHV-6 isolate U1102 belonging to variant A. All the sera showed a similar reactivity.
authors.affiliation: Institute of Hygiene, University of Modena, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Cermelli
authors.forename: A
authors.initials: A
authors.lastname: Moroni
authors.forename: M
authors.initials: M
authors.lastname: Montorsi
authors.forename: G
authors.initials: G
authors.lastname: Fabio
authors.forename: M
authors.initials: M
authors.lastname: Portolani
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006566:Herpesviridae Infections / Q000276:immunology; D015654:Herpesvirus 6, Human / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G