A synthetic peptide deduced from the sequence in the cross-region of laminin A chain mediates neurite outgrowth, cell attachment and heparin binding.
Synthetic peptides from the cross-region of the laminin A chain were prepared and tested for their biological activities, especially for neurite outgrowth. A synthetic 8-mer peptide (designated LMA-5) in the cross-region of the laminin A chain was found to promote neurite outgrowth in PC12 cells and cerebellar microexplant cultures. Furthermore, this peptide mediated cell attachment and heparin binding. In addition, an antibody against peptide LMA-5 inhibited laminin- and LMA-5-mediated cell attachment. This antibody also inhibited more than half of the LMA-5-promoted neurite outgrowth in cerebellar microexplant cultures. These data suggest that peptide LMA-5 is one of the active sites in laminin that regulate cell behaviour including neurite outgrowth, cell attachment and heparin binding.
Tashiro
K
K
Department of Medical Technology, School of Health Sciences, Kyushu University, Fukuoka, Japan.
Nagata
I
I
Yamashita
N
N
Okazaki
K
K
Ogomori
K
K
Tashiro
N
N
Anai
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion / Q000187:drug effects*; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D007797:Laminin / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D007074:Immunoglobulin G; D007797:Laminin; D010446:Peptide Fragments; C084098:laminin A; D006493:Heparin