title: Hepatocyte growth factor/scatter factor modulates intestinal epithelial cell proliferation and migration.
abstract: Various peptide growth factors have been found to regulate epithelial cell function within the mucosal epithelium of the gastrointestinal tract. In this study hepatocyte growth factor/scatter factor (HGF/SF) was found to stimulate intestinal epithelial cell proliferation: 2.5-fold in the non-transformed rat small intestinal epithelial cell line IEC-6 and 1.9-fold in the human colon cancer-derived HT-29 cell line. In addition, HGF/SF enhanced epithelial cell restitution, the initial step involved in gastrointestinal wound healing, in an in vitro model. Migration of IEC-6 in wounded monolayers was enhanced up to 7-fold. Enhancement of restitution by HGF could be completely abrogated by addition of immunoneutralizing anti-TGF beta 1, indicating that this process is mediated through a TGF beta-dependent pathway. These findings suggest that HGF exerts functional effects on intestinal epithelial cell populations and may play a role in the morphogenesis of the gastrointestinal tract and its remodeling following injury.
authors.affiliation: Department of Medicine, Massachusetts General Hospital, Boston 02114.
authors.forename: A U
authors.initials: AU
authors.lastname: Dignass
authors.forename: K
authors.initials: K
authors.lastname: Lynch-Devaney
authors.forename: D K
authors.initials: DK
authors.lastname: Podolsky
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002465:Cell Movement; D003110:Colonic Neoplasms; D004847:Epithelial Cells; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D007422:Intestines / Q000166:cytology*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D017228:Hepatocyte Growth Factor