Transformation of wheat protoplasts mediated by cationic liposome and regeneration of transgenic plantlets.
E. coli plasmid pCGN1055 containing the hygromycin phosphotransferase (Hpt) gene under the control of the plant promoter was introduced into wheat protoplasts by cationic liposome (lipofectin)-mediated transformation, and transgenic albino plantlets were obtained. The transformation frequencies were 6.0% and 8.8% according to hygromycin resistance and DNA molecular hybridization. The assay of Hpt enzyme activity and Southern blot indicated the foreign gene existed and expressed in transgenic plantlets.
Zhu
Z
Z
Institute of Genetics, Chinese Academy of Sciences, Beijing.
Sun
B
B
Liu
C
C
Xiao
G
G
Li
X
X
D002412:Cations; D008081:Liposomes; D010714:Phosphatidylethanolamines; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D030821:Plants, Genetically Modified; D011523:Protoplasts; D012038:Regeneration; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
D002412:Cations; D008081:Liposomes; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase