Species-dependent expression of the hyoscyamine 6 beta-hydroxylase gene in the pericycle.
The tropane alkaloid scopolamine is synthesized in the pericycle of branch roots in certain species of the Solanaceae. The enzyme responsible for the synthesis of scopolamine from hyoscyamine is hyoscyamine 6 beta-hydroxylase (H6H). The gene for H6H was isolated from Hyoscyamus niger. It has an exon/intron organization very similar to those for ethylene-forming enzymes, suggesting a common evolutionary origin. The 827-bp 5' flanking region of the H6H gene was fused to the beta-glucuronidase (GUS) reporter gene and transferred to three solanaceous species by Agrobacterium-mediated transformation systems: H. niger and belladonna (Atropa belladonna), which have high and low levels, respectively, of H6H mRNA in the root, and tobacco (Nicotiana tabacum), which has no endogenous H6H gene. Histochemical analysis showed that GUS expression occurred in the pericycle and at the root meristem of transgenic H. niger hairy roots, but only at the root meristem of transgenic H. niger hairy roots, but only at the root meristem of hairy roots and plants of transgenic tobacco. In transgenic hairy roots and regenerated plants of belladonna, the root meristem was stained with GUS activity, except for a few transformants in which the vascular cylinder was also stained. These studies indicate that the cell-specific expression of the H6H gene is controlled by some genetic regulation specific to scopolamine-producing plants.
Kanegae
T
T
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Japan.
Kajiya
H
H
Amano
Y
Y
Hashimoto
T
T
Yamada
Y
Y
D000595:Amino Acid Sequence; D001532:Atropa belladonna / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D015870:Gene Expression; D017343:Genes, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D006651:Histocytochemistry; D007438:Introns; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal / Q000201:enzymology* / Q000235:genetics*; D010947:Plants, Toxic; D013045:Species Specificity; D014026:Nicotiana / Q000235:genetics
D017931:DNA Primers; D004247:DNA; D006899:Mixed Function Oxygenases; C052273:hyoscyamine (6S)-dioxygenase; D005966:Glucuronidase