Expression of xenobiotic-metabolizing cytochrome P450 forms in human adult and fetal liver.
Expression of human cytochrome P450 (CYP) genes in human adult and fetal liver were studied using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. In adult liver mRNA of CYPs 1A1, 1A2, 2A6/2A7, 2B6/2B7, 2C8-19, 2D6, 2E1, 3A3/3A4 and 3A7 were detected while CYPs 2F1 and 4B1 were absent. In fetal liver mRNA of CYPs 2C8, 2D6, 3A3/3A4 and 3A7 were found but all other forms studied were undetectable. The results provide a comprehensive qualitative picture of the expression of CYP genes in families CYP1 through CYP4 in human adult and fetal liver.
Hakkola
J
J
Department of Pharmacology and Toxicology, University of Oulu, Finland.
Pasanen
M
M
Purkunen
R
R
Saarikoski
S
S
Pelkonen
O
O
Mäenpää
J
J
Rane
A
A
Raunio
H
H
D000328:Adult; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D015262:Xenobiotics / Q000378:metabolism*
D017931:DNA Primers; D007527:Isoenzymes; D012333:RNA, Messenger; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System