title: MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast.
abstract: The discovery that mutations in DNA mismatch repair genes can cause hereditary nonpolyposis colorectal cancer has stimulated interest in understanding the mechanism of DNA mismatch repair in eukaryotes. In the yeast Saccharomyces cerevisiae, DNA mismatch repair requires the MSH2, MLH1, and PMS1 proteins. Experiments revealed that the yeast MLH1 and PMS1 proteins physically associate, possibly forming a heterodimer, and that MLH1 and PMS1 act in concert to bind a MSH2-heteroduplex complex containing a G-T mismatch. Thus, MSH2, MLH1, and PMS1 are likely to form a ternary complex during the initiation of eukaryotic DNA mismatch repair.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06511.
authors.forename: T A
authors.initials: TA
authors.lastname: Prolla
authors.forename: Q
authors.initials: Q
authors.lastname: Pang
authors.forename: E
authors.initials: E
authors.lastname: Alani
authors.forename: R D
authors.initials: RD
authors.lastname: Kolodner
authors.forename: R M
authors.initials: RM
authors.lastname: Liskay
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D002846:Chromatography, Affinity; D004260:DNA Repair; D004261:DNA Replication; D004271:DNA, Fungal / Q000378:metabolism*; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000378:metabolism*; D008957:Models, Genetic; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D051718:MutS Homolog 2 Protein; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C088859:MLH1 protein, S cerevisiae; D009692:Nucleic Acid Heteroduplexes; C088858:PMS1 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C489751:MSH2 protein, S cerevisiae; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D051718:MutS Homolog 2 Protein