Human hepatic uptake of two vinca alkaloids: navelbine and vincristine.
A human liver plasma membrane model for the evaluation of the specific binding and transport processes of drugs presenting high hepatic clearance such as vinca alkaloids was developed. Uptake of the two structural antitumor analogs, navelbine (NVB) and vincristine (VCR), which exhibit wide variabilities in their respective pharmacokinetic parameters and antitumor spectra, was investigated. The high yield, the enzymatic profile and the retention of physiologic transport capacities, as demonstrated by taurocholate uptake, revealed that this membrane preparation was well suited for studies of hepatic drug transport systems. For both drugs two distinct processes were observed: mainly membrane binding and transport. NVB was found to bind to the membrane vesicles more intensively than VCR, but the transport processes were almost identical. However only NVB uptake seems to involve Na(+)-dependent processes. These significant differences may be related to the respective lipophilicity of the drugs. The more lipophilic molecule (NVB) presents the highest uptake, which is presumably at the origin of its greatest distribution volume in vivo.
Rahmani-Jourdheuil
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D
INSERM U 278, Faculté de Pharmacie, Marseille, France.
Coloma
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F
Placidi
M
M
Rahmani
R
R
D000970:Antineoplastic Agents / Q000493:pharmacokinetics*; D015415:Biomarkers; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D013656:Taurocholic Acid / Q000493:pharmacokinetics; D013696:Temperature; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D014750:Vincristine / Q000493:pharmacokinetics*; D000077235:Vinorelbine
D000970:Antineoplastic Agents; D015415:Biomarkers; D013656:Taurocholic Acid; D014750:Vincristine; D014747:Vinblastine; D000077235:Vinorelbine