Gene transfer of TNF receptor for treatment of cancer by TNF.
Tumor necrosis factor-alpha receptors (TNFR-55 and TNFR-75) were inserted into retrovirus derived vector. They were transfected into a packaging cell line. The high titer of transfectants, which produced virus containing TNFR-55 or TNFR-75, was obtained. TNFR-55 and TNFR-75 negative human colon cancer cells were infected with this virus as a model experiment of gene therapy. Although the original colon cancer cell line did not express TNFR-55 or TNFR-75, the colon cancer cells, which were infected by recombinant virus, expressed a high level of TNFR-55 or TNFR-75. TNFR-55 or TNFR-75 transformed colon cancer cells were killed by recombinant TNF.
Isobe
K
K
Department of Immunology, Nagoya University School of Medicine, Japan.
Fan
Z H
ZH
Emi
N
N
Nakashima
I
I
D002460:Cell Line; D003110:Colonic Neoplasms / Q000628:therapy*; D018076:DNA, Complementary; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000627:therapeutic use*
D018076:DNA, Complementary; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha