Plasma 17-hydroxyprogesterone determination with two commercial immunoassays.
Plasma 17-hydroxyprogesterone (17-OH-P) was determined by two commercially available immunoassay kits, a radioimmunoassay (RIA) (OHP-CT, CIS) and an enzyme-immunoassay (EIA) (Serozyme 17 alpha-OH-progesterone, Serono). The determination by RIA was performed according to two procedures, directly on plasma or on a crude plasma extract, whereas that by EIA used only the second procedure. These determinations were carried out in 27 infants below 1 year of age and in 33 women in the follicular phase of the menstrual cycle. The results were compared to those obtained by an in-home RIA (RIA-FRH) which includes an extraction step followed by chromatography on Sephadex LH 20 column. The levels observed were overestimated by both kits. In infants, interference from 17-hydroxy-pregnenolone (17-OH-5P) sulfate occurred when the RIA (CIS) kit was used directly on plasma samples. Using plasma extracts, 17-OH-5P interfered with EIA (Serono) in the infant group and with the RIA (CIS) in the second group. The two kits do not appear to be adequate for 17-OH-P determination at least in infants and in women in the follicular phase of the menstrual cycle.
Nahoul
K
K
Foundation for Hormone Research, Fresnes, France.
D019326:17-alpha-Hydroxyprogesterone; D000328:Adult; D005260:Female; D005498:Follicular Phase; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood*; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008598:Menstruation; D011863:Radioimmunoassay
D006908:Hydroxyprogesterones; D019326:17-alpha-Hydroxyprogesterone