Detection of hepatitis B virus DNA and determination of surface antigen expression in peripheral blood mononuclear cells from patients with AIDS.
The polymerase chain reaction (PCR) was used to analyze the presence of hepatitis B virus (HBV) DNA in serum and peripheral blood mononuclear cells (PBMCs) from 20 patients with AIDS with and without conventional HBV serological markers. DNA sequences of HBV were detected in PBMCs from 13 patients, nine of whom were positive for anti-HBc only and four of whom were also positive for anti-HBs. When PBMCs from patients were activated in culture with phytohemagglutinin, the presence of HBsAg could be detected in the culture supernatants from four of 13 patients with HBV DNA in their PBMCs; for two of the four, HBV DNA could also be detected in the culture supernatant after DNA amplification. It was observed that HBV DNA sequences found in PBMCs can be reactivated by mitogen stimulation in some HIV-1 infected patients.
Malavé Lara
C
C
Microbiology and Immunology Department, University of Basque Country, Bilbao, Spain.
Gorriño
M T
MT
Campelo
C
C
Lardelli
P
P
Cisterna
R
R
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000382:microbiology*; D000328:Adult; D001483:Base Sequence; D002478:Cells, Cultured; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification; D006515:Hepatitis B virus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins / Q000494:pharmacology; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D010835:Phytohemagglutinins