Characterization of R plasmids from Shigella sonnei in Taiwan.
Plasmids of 70MDa from 6 Shigella sonnei isolates originating in Kaohsiung, Taiwan were found. All of them encoded resistance to chloramphenicol and tetracycline. These plasmids belonged to the H II incompatibility group. The fertility inhibition property of plasmids was the fi- type. Restriction endonuclease analysis revealed the six plasmids all had identical restriction patterns with BamH I, Hind III and Nru I. All six plasmids carried the type I chloramphenicol acetyltransferase gene and the class B tetracycline resistance gene as determined by Southern hybridization with DNA probes of various antimicrobial resistance genes. The results demonstrated that S. sonnei isolates harboured the same R plasmid.
Chang
C Y
CY
Department of Microbiology, Kaohsiung Medical College, Taiwan, Republic of China.
Chang
L L
LL
Lin
S R
SR
Chang
S F
SF
D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D011815:R Factors; D015183:Restriction Mapping; D012764:Shigella sonnei / Q000187:drug effects / Q000235:genetics*
D015500:Chloramphenicol O-Acetyltransferase