The fractionation of human plasma proteins. III. Purification of complement factors D and I using affinity chromatography.
A purification protocol for complement factors D and I was designed as part of a larger scheme for the purification of more than 20 human plasma proteins. The key affinity step in the purification of both serine proteases, factors D and I, was affinity chromatography using benzamidine Sepharose. The recoveries were 19 and 12% for factors D and I, respectively.
DiScipio
R G
RG
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
D001550:Benzamidines; D001798:Blood Proteins / Q000302:isolation & purification; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid / Q000379:methods; D011416:Complement Factor D / Q000302:isolation & purification*; D017244:Complement Factor I / Q000302:isolation & purification*; D016264:Dextran Sulfate; D006801:Humans
D001550:Benzamidines; D001798:Blood Proteins; D016264:Dextran Sulfate; D017244:Complement Factor I; C493488:CFD protein, human; D011416:Complement Factor D; C032157:benzamidine