Bacterial expression of a soluble T-cell receptor alpha chain.
A bacterial expression system was used to express the complete extracellular domain of a rat T-cell receptor alpha-chain, including variable-, joining and constant regions. By introduction of a His-tail at the C-terminus, a simple and rapid purification protocol utilizing Ni(+)-chelating chromatography was applied, with renaturation of the protein while bound to the matrix. This method produced large amounts of soluble protein, which provide a source for T-cell receptor analysis and further studies on T cell function.
Stangel
M
M
Department of Neurology, Julius-Maximilians Universität, Würzburg, Germany.
Toyka
K V
KV
Hartung
H P
HP
Giegerich
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D051381:Rats; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics*; D012995:Solubility
D004274:DNA, Recombinant; D016693:Receptors, Antigen, T-Cell, alpha-beta