No high affinity melatonin binding sites are detected in murine melanoma cells and in normal human melanocytes cultured in vitro.
Saturation studies of 2-(125I)-iodomelatonin binding to membranes from normal human melanocytes, mouse melanoma cells B16F10 and amelanotic S91 revealed no specific binding. Using 2-(125I)-iodomelatonin in the concentration range of 6 to 566 pM, no high affinity melatonin binding sites were detectable in any of the cell types. Even when the concentration of radioligand was increased up to 2000 pM, specific binding was either low or absent and not reproducible. These results suggest that in the culture conditions used in this study, no high affinity melatonin binding sites are detected in pigmented cells.
Mengeaud
V
V
INSERM U385, Faculté de Medecine, Nice, France.
Skene
D
D
Pevet
P
P
Ortonne
J P
JP
D000818:Animals; D001665:Binding Sites; D002645:Chickens; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008546:Melanoma, Experimental / Q000378:metabolism*; D008550:Melatonin / Q000031:analogs & derivatives / Q000378:metabolism*; D051379:Mice; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D007457:Iodine Radioisotopes; C044556:2-iodomelatonin; D008550:Melatonin