Glucose and insulin responses in isolated human lymphocytes reflect in vivo status: effects of VLCD treatment.
Human lymphocyte growth response to mitogen was examined in vitro under different conditions to monitor aspects of carbohydrate utilization in three groups: obese nondiabetic, obese/NIDDM, and normal-weight individuals. Although lymphocyte growth capacity in minimal medium for these three groups was found to be similar at a glucose concentration of 72 micrograms/ml in the absence of insulin or glycerol, differences in mitogen-stimulated growth were observed at lower glucose concentrations or in the presence of insulin or glycerol. Interestingly, these metabolic alterations in lymphocytes can be normalized by treating obese and obese/NIDDM subjects with a very low calorie diet (VLCD) regimen. The results of this study indicate that lymphocyte culture in a defined medium may provide a mechanism to examine different metabolic states and to evaluate treatment regimens (diet, exercise, etc.) for obese and NIDDM subjects.
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Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251.
Matthews
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Topek
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Matthews
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D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003920:Diabetes Mellitus / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D002149:Energy Intake; D005947:Glucose / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D009765:Obesity / Q000097:blood*
D001786:Blood Glucose; D007328:Insulin; D005947:Glucose; D005990:Glycerol