title: Cultured trout liver cells: utilization of substrates and response to hormones.
abstract: The characterization of a recently established system for the short-term culture of rainbow trout (Oncorhynchus mykiss) liver cells in chemically defined medium has been extended to studies on the metabolic competence of the cells and the characterization of their response to hormones. Three areas of metabolism have been addressed: a) the utilization of the exogenously added substrates fructose, lactate, glucose, dihydroxyacetone, and glycerol for glucose and lactate formation; b) the effects of the pancreatic hormones insulin and glucagon on cellular glucose formation, lactate formation, and fatty acid synthesis; and c) the effects of insulin and dexamethasone on the estradiol-dependent production of vitellogenin. Incubation of trout liver cells with fructose, lactate, glucose, dihydroxyacetone, or glycerol resulted in enhanced rates of cellular glucose and lactate production. Substrate-induced effects usually were more clearly expressed after extended (20 h) than after acute (5 h) culture periods. Addition of the hormones insulin or glucagon caused dose-dependent alterations in the flux of substrates to glucose and lactate. Rates of de novo synthesis of fatty acids from [14C]acetate were stimulated by insulin and inhibited by glucagon during acute and extended incubation periods. Treatment of liver cells isolated from male trout for 72 h with estradiol induced vitellogenin production and secretion into the medium. However, the addition of insulin or dexamethasone drastically reduced this estrogen-induced vitellogenesis. These results indicate that trout liver cells cultured in defined medium maintain central metabolic pathways, including glycolysis, gluconeogenesis, lipogenesis, and vitellogenesis as well as their responsiveness to various hormones, for at least 72 h.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Zoology II, University of Karlsruhe, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Segner
authors.forename: J B
authors.initials: JB
authors.lastname: Blair
authors.forename: G
authors.initials: G
authors.lastname: Wirtz
authors.forename: M R
authors.initials: MR
authors.lastname: Miller
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005934:Glucagon / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008055:Lipids / Q000096:biosynthesis; D008099:Liver / Q000166:cytology*; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D017686:Oncorhynchus mykiss; D014818:Vitellogenesis
chemical_list: D007328:Insulin; D007773:Lactates; D008055:Lipids; D008112:Liver Glycogen; D019344:Lactic Acid; D005934:Glucagon; D005947:Glucose