title: Ultrastructure of developing kidney glomerular basement membranes: temporal changes in binding of anti-laminin IgG and cationized ferritin.
abstract: In vivo labeling of infant rat and mouse glomerular basement membranes (GBMs) with polyclonal anti-laminin IgGs results in binding across the full widths of GBMs at all stages of development. These stages include the pre-fusion, double basement membranes found beneath endothelial cells and podocytes in early glomeruli, and the subepithelial matrix outpockets where newly synthesized GBM is spliced into fused basement membrane during glomerular maturation. Identical binding results are obtained either with peroxidase or post-embedding immunogold techniques. Although injected cationized ferritin also binds abundantly to all developing GBMs, it quickly disappears and, 24 hours after injection, is generally absent from GBMs but remains within mesangial matrices. Injection of newborn mice with monoclonal anti-laminin IgGs results in dense labeling of pre-fusion GBMs but post-fusion GBMs and subepithelial outpockets are weak-negative. Although masking can not be excluded, these results indicate that laminin epitopes are removed during GBM fusion and splicing, either by isoform substitution or proteolytic processing. The loss of bound cationized ferritin is believed to occur mainly through rapid turnover of GBM proteoglycans.
authors.affiliation: Department of Cell Biology, School of Medicine, University of Alabama at Birmingham 35294-0019.
authors.forename: D R
authors.initials: DR
authors.lastname: Abrahamson
authors.forename: P L
authors.initials: PL
authors.lastname: St John
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*; D005293:Ferritins / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*; D007797:Laminin / Q000276:immunology*; D008024:Ligands; D051379:Mice; D051381:Rats
chemical_list: D007074:Immunoglobulin G; D007797:Laminin; D008024:Ligands; C010297:polycationic ferritin; D005293:Ferritins