Optimal conditions of 1 M NaCl splitting technique to demonstrate basement membrane zone antigens in bullous pemphigoid, epidermolysis bullosa acquisita and linear IgA bullous dermatoses.
We compare a series of conditions for inducing lamina lucida separation through normal skin specimens using 1 M NaCl for the detection of basement membrane zone auto-antibodies using indirect immunofluorescence. Skin was split at room temperature and at 4 degrees C with and without phenylmethylsulphonyl fluoride. Our results demonstrate that for the detection of bullous pemphigoid and epidermal-binding linear IgA bullous dermatoses antibodies skin should be split at 4 degrees C with enzyme inhibitors. For epidermolysis bullosa acquisita and dermal-binding antibodies skin can be split at room temperature.
Jenkins
R E
RE
St. John's Institute of Dermatology, St. Thomas' Hospital, London, UK.
Rodenas
J
J
Bhogal
B S
BS
Black
M M
MM
D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis*; D001485:Basement Membrane / Q000276:immunology; D016107:Epidermolysis Bullosa Acquisita / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006652:Histological Techniques; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D010391:Pemphigoid, Bullous / Q000276:immunology; D012867:Skin / Q000276:immunology*; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology*; D012965:Sodium Chloride
D001323:Autoantibodies; D001324:Autoantigens; D007070:Immunoglobulin A; D012965:Sodium Chloride