Isolation and structure of the rat gene encoding troponin I(slow).
Troponin I is a myofibrillar protein involved in the Ca(2+)-mediated regulation of actomyosin ATPase activity. We report here the isolation and characterization of the gene coding for the slow-muscle-specific isoform of the rat troponin I polypeptide (TpnI). Using restriction mapping, PCR mapping and partial DNA sequencing, we have determined the exon/intron arrangement of this gene. The transcription unit is 10.5-kb long and contains nine exons ranging in size from 4 bp to 330 bp. The rat TpnI(slow) gene is interrupted by large intervening sequences; a 3.3-kb intron separates the 5' untranslated exons from the protein-coding exons. Comparison of the structure of rat TpnI(slow) with that of quail TpnI(fast) reveals that they have a similar intron/exon organization. The 5' untranslated region of the rat gene contains an additional exon, otherwise, the positions of introns and coding exons map to essentially identical regions in both genes.
Banerjee-Basu
S
S
Unit on Molecular Neurobiology, National Institutes of Health, Bethesda, MD 20892.
Buonanno
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005091:Exons / Q000235:genetics; D015698:Genomic Library; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014336:Troponin / Q000145:classification / Q000235:genetics*; D019210:Troponin I
D014336:Troponin; D019210:Troponin I