The p15gag and p12gag regions are both necessary for the pathogenicity of the murine AIDS virus.
The defective murine AIDS (MAIDS) virus has unique sequences in its p15gag and p12gag regions. To clarify whether these sequences are responsible for the development of MAIDS, we constructed recombinant viruses by replacing various regions of the gag gene of the nonpathogenic replication-competent LP-BM5 ecotropic virus with those of the MAIDS virus. Recombinants containing both unique sequences of the MAIDS virus were replication defective and induced MAIDS. However, a recombinant containing either the p15gag or p12gag region of the MAIDS virus was also replication defective but nonpathogenic in mice. A recombinant virus containing only the p30gag region of the MAIDS virus was replication competent and nonpathogenic. These results indicate that the p15gag and p12gag regions of the MAIDS virus do not function like those of replication-competent viruses and that both of the unique sequences in the p15gag and p12gag regions are required to develop MAIDS.
Kubo
Y
Y
Department of Viral Oncology, Kyoto University, Japan.
Kakimi
K
K
Higo
K
K
Wang
L
L
Kobayashi
H
H
Kuribayashi
K
K
Masuda
T
T
Hirama
T
T
Ishimoto
A
A
D000818:Animals; D001483:Base Sequence; D015683:Gene Products, gag / Q000502:physiology*; D009052:Leukemia Virus, Murine / Q000472:pathogenicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016183:Murine Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D011993:Recombinant Fusion Proteins; D014779:Virus Replication
D015683:Gene Products, gag; D011993:Recombinant Fusion Proteins