Two-dimensional gel mapping of small GTPases reveals transformation-specific changes during oncogenesis.
Epithelial cells transformed by oncogenes in vitro change their gene expression program, thereby losing features of cell polarity and cell adhesion. Using ras-transformed mammary epithelial cells, we have investigated the expression of other small GTP-binding proteins by high-resolution two-dimensional gel electrophoresis and direct GTP ligand binding after renaturing transfer onto nitrocellulose. Ras-transformed cells lost the expression of one epithelial-specific GTP-binding protein (21-22 kDa, pI 4.5-4.8) and instead expressed a fibroblast GTP-binding protein (21-22 kDa, pI 4.8-5.0).
Huber
L A
LA
European Molecular Biology Laboratory, Cell Biology Program, Heidelberg.
Beug
H
H
Simons
K
K
Reichmann
E
E
D000818:Animals; D001345:Autoradiography / Q000379:methods; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004848:Epithelium; D020558:GTP Phosphohydrolases / Q000096:biosynthesis* / Q000302:isolation & purification*; D011905:Genes, ras; D006160:Guanosine Triphosphate / Q000378:metabolism; D007668:Kidney; D008321:Mammary Glands, Animal; D051379:Mice; D010761:Phosphorus Radioisotopes
D010761:Phosphorus Radioisotopes; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases