title: Vasopressin antisense oligonucleotide induces temporary diabetes insipidus in rats.
abstract: The purpose of this study was to downregulate the transcriptional message of arginine vasopressin (AVP) by antisense treatment. A complete phosphorothioate antisense oligodesoxynucleotide corresponding to the beginning of the coding region of rat AVP mRNA was constructed and injected into the lateral ventricle of rats. Within 3-6 h animals exhibited a temporary diabetes insipidus, which lasted up to 9 h. Accordingly, vasopressin immunoreactivity in the hypothalamic nuclei was reduced. Our results demonstrate that a specific and reversible inhibition of neuropeptide expression can be accomplished in the intact hypothalamo-neurohypophysial system by antisense treatment, thus providing a novel tool for studies on stimulus-secretion coupling in vivo.
authors.affiliation: Department of Neuroendocrinology, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Skutella
authors.forename: J C
authors.initials: JC
authors.lastname: Probst
authors.forename: M
authors.initials: M
authors.lastname: Engelmann
authors.forename: C T
authors.initials: CT
authors.lastname: Wotjak
authors.forename: R
authors.initials: R
authors.lastname: Landgraf
authors.forename: G F
authors.initials: GF
authors.lastname: Jirikowski
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis*; D001483:Base Sequence; D003919:Diabetes Insipidus / Q000139:chemically induced* / Q000652:urine; D015536:Down-Regulation / Q000187:drug effects; D007276:Injections, Intraventricular; D008297:Male; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000494:pharmacology*; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016376:Oligonucleotides, Antisense; D001127:Arginine Vasopressin