Studies on the effect of an heterologous fatty acid-binding protein on acyl-CoA oxidase induction in Saccharomyces cerevisiae.
The participation of fatty acid-binding protein (FABP) in the induction of peroxisomal beta-oxidation of fatty acids was investigated in vivo in an heterologous system. Bovine heart FABP was expressed in Saccharomyces cerevisiae under the control of two different promoters: a constitutive one and an oleic acid-inducible one. Constructs were introduced into yeast cells on multicopy and integrating plasmids. The heterologous FABP was present in yeast cells in two isoforms having pI values of about 5 and was able to bind oleic acid. The heterologous FABP had no significant effect on acyl-CoA oxidase activity at various concentrations of the inducing agent.
Smaczyńska
I
I
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa.
Skoneczny
M
M
Kurlandzka
A
A
D042962:Acyl-CoA Oxidase; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D050556:Fatty Acid-Binding Proteins; D015870:Gene Expression; D018014:Gene Transfer Techniques; D007525:Isoelectric Focusing; D009206:Myocardium / Q000737:chemistry; D009363:Neoplasm Proteins; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins; D012440:Saccharomyces / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009829:Oleic Acids; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D019301:Oleic Acid; D010088:Oxidoreductases; D002374:Catalase; D042962:Acyl-CoA Oxidase; C467991:POX1 protein, S cerevisiae