Molecular cloning and sequence analysis of a cDNA encoding a molt-inhibiting hormone-like neuropeptide from the white shrimp Penaeus vannamei.
The X-organ of the eyestalk in crustaceans is a source of neurosecretory peptides. Total RNA was isolated from X-organs of the white shrimp Penaeus vannamei and used to clone a cDNA encoding a molt-inhibiting hormone-like (MIH-like) neuropeptide by the 3' and 5' rapid amplification of cDNA ends (RACE) method. Sequence analysis of the 470-bp cDNA reveals a 306-bp open reading frame and a 164-bp 3' untranslated region. The deduced polypeptide consists of a 72-amino acid mature peptide and a 30-amino acid region of propeptide. The mature peptide shares 49 and 29% amino acid identity to the MIH from the lobster Homarus americanus and the crab Carcinus maenas, respectively. Northern hybridization shows that the MIH-like mRNA has a molecular size of 2.0 kb and is present in the eyestalk.
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Molecular Endocrinology Program, Pacific Biomedical Research Center, University of Hawaii, Honolulu 96822.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D007447:Invertebrate Hormones / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics* / Q000302:isolation & purification; D033561:Penaeidae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D007447:Invertebrate Hormones; D009479:Neuropeptides; C012445:molt-inhibiting hormone; C088772:molt-inhibiting hormone-like neuropeptide