Inhibition of purine nucleobase transport in human erythrocytes and cell lines by papaverine. Investigation of structure-activity relationship.
Papaverine was found to be an effective inhibitor of hypoxanthine transport not only in human erythrocytes, but also in the human cell lines HL60 (myeloic) and U937 (monocytic). IC50 values for inhibition of hypoxanthine influx ranged from 6 to 20 microM. In erythrocytes papaverine was found to be a non-competitive inhibitor of hypoxanthine equilibrium-exchange transport with a Ki value of approximately 13 microM, which is in close agreement with the respective IC50 values estimated for zero-trans influx of hypoxanthine. In addition papaverine also had a slight inhibitory effect on unmediated nucleobase transport, most likely due to a perturbation of the membrane lipid environment. Several papaverine analogs were tested for their inhibitory effect on nucleobase transport. Only ethaverine was as effective as papaverine. Drotaverine and berberine were moderately inhibitory while laudanosine had no inhibitory effect at all. Isoquinoline acted as a very weak inhibitor.
Kraupp
M
M
Institut für Medizinische Chemie der Universität Wien, Vienna, Austria.
Paskutti
B
B
Schön
C
C
Marz
R
R
D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D007700:Kinetics; D010208:Papaverine / Q000031:analogs & derivatives / Q000494:pharmacology*; D011684:Purine Nucleosides / Q000378:metabolism*; D013329:Structure-Activity Relationship
D007042:Hypoxanthines; D011684:Purine Nucleosides; D019271:Hypoxanthine; D010208:Papaverine