Conditioned media of glial cell lines induce alkaline phosphatase activity in cultured artery endothelial cells. Identification of interleukin-6 as an induction factor.
Conditioned media of human glial cell lines induced alkaline phosphatase activity in cultured calf artery endothelial cells. The maximal alkaline phosphatase activity in the culture was comparable to the level in isolated brain capillary endothelial cells. An induction factor in the conditioned media was purified and identified as interleukin-6 from its amino-terminal sequence, molecular weight, amino acid composition and immunoreactivity. Recombinant interleukin-6 had similar induction activity. Our findings raise the possibility that interleukin-6 induces and modulates alkaline phosphatase activity in endothelial cells during normal development of the blood-brain barrier and under certain pathological conditions.
Takemoto
H
H
Shionogi Discovery Research Laboratories II, Shionogi & Co. Ltd., Osaka, Japan.
Kaneda
K
K
Hosokawa
M
M
Ide
M
M
Fukushima
H
H
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000201:enzymology*; D004790:Enzyme Induction; D006801:Humans; D015850:Interleukin-6 / Q000502:physiology*; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*; D011651:Pulmonary Artery / Q000201:enzymology; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D015850:Interleukin-6; D011994:Recombinant Proteins; D000469:Alkaline Phosphatase