Gq and G11 are concurrently activated by bombesin and vasopressin in Swiss 3T3 cells.
The alpha-subunits of the widely expressed G-proteins Gq and G11 indistinguishably activate beta-isoforms of phospholipase C. In this report we have tested whether differences exist in the activation of both G-proteins via phospholipase C-linked receptors. We found that bombesin and vasopressin, with very similar potencies and time dependencies, induce the activation of both Gq and G11 in Swiss 3T3 cells, suggesting that these G-proteins, at least in part, serve interchangable functions.
Offermanns
S
S
Institut für Pharmakologie, Freie Universität Berlin, Germany.
Heiler
E
E
Spicher
K
K
Schultz
G
G
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001839:Bombesin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010738:Type C Phospholipases / Q000378:metabolism*; D014667:Vasopressins / Q000378:metabolism*
D014667:Vasopressins; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins; D001839:Bombesin