Transformation of Mycoplasma capricolum and examination of DNA restriction modification in M. capricolum and Mycoplasma mycoides subsp. mycoides.
Plasmids pIK delta and pIK delta-erm have recently been developed as mycoplasmal cloning vectors. In this report, we demonstrate that these plasmids can replicate in Mycoplasma capricolum, a mycoplasmal species for which transformation had not previously been characterized. Both plasmids are stably maintained at a higher copy number than in their parental species, Mycoplasma mycoides subsp. mycoides. We have also examined the possibility of one or more restriction-modification systems affecting transformation frequencies in both species.
King
K W
KW
Department of Comparative Medicine, University of Alabama at Birmingham 35294.
Dybvig
K
K
D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015247:Deoxyribonuclease HindIII; D005822:Genetic Vectors; D009174:Mycoplasma / Q000235:genetics*; D009176:Mycoplasma mycoides / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D015247:Deoxyribonuclease HindIII