title: Up-regulation of Na+,K+ adenosine triphosphatase after massive intestinal resection.
abstract: BACKGROUND
  The mechanisms of intestinal adaptation after resection are not completely defined. The purpose of this study was to examine the changes after resection in the enterocyte basolateral Na+,K+ adenosine triphosphatase (ATPase) known to play a critical role in epithelial transport and homeostasis.
  
  
  METHODS
  Lewis rats underwent 70% small bowel resection or transection. At 6 hours, 24 hours, 1 week, and 2 weeks, jejunum and ileum were harvested for analysis of Na+,K+ ATPase activity, kinetic analysis, and alpha 1-ATPase messenger RNA and protein levels.
  
  
  RESULTS
  Na+,K+ ATPase activity increased (p < 0.05) in both the jejunum and ileum by 2 weeks after resection. This rise in activity correlated with an increase in the maximal activity of ATPase, from 20.8 to 101.01 mumol inorganic phosphate.mg-1.hr-1. ATPase messenger RNA levels increased sixfold in the jejunum and tenfold in the ileum by 2 weeks after resection (p < 0.05). Protein levels rose at 6 hours and remained elevated in both tissues.
  
  
  CONCLUSIONS
  After intestinal resection, enterocyte Na+,K+ ATPase activity rises as a result of an increase in the number of transporters per cell. This occurs through both transcriptional and translational mechanisms. It appears that intestinal adaptation after resection involves not only an increase in absorptive surface area but also functional adaptation by the individual enterocyte.
authors.affiliation: Department of Surgery, University of California, Los Angeles.
authors.forename: O J
authors.initials: OJ
authors.lastname: Hines
authors.forename: A J
authors.initials: AJ
authors.lastname: Bilchik
authors.forename: D W
authors.initials: DW
authors.lastname: McFadden
authors.forename: M J
authors.initials: MJ
authors.lastname: Skotzko
authors.forename: E E
authors.initials: EE
authors.lastname: Whang
authors.forename: M J
authors.initials: MJ
authors.lastname: Zinner
authors.forename: S W
authors.initials: SW
authors.lastname: Ashley
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001483:Base Sequence; D007422:Intestines / Q000201:enzymology* / Q000601:surgery*; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase