title: Regional myocardial flow and capillary permeability-surface area products are nearly proportional.
abstract: Analyses of data on the transcapillary exchange and cellular uptake in the normal heart have generally been based on the assumption that local membrane conductances and volumes of distribution are everywhere the same. The question is whether such an assumption is justified in view of the marked (sixfold) heterogeneity of local blood flows per gram tissue. The method was to estimate both flow and capillary membrane permeability-surface area products (PS) locally in the heart. For each of five dogs running on a sloped treadmill, the deposition of tracer microspheres and of [131I]iodophenylpentadecanoic acid (IPPA), after left atrial injection, was determined in 256 pieces of left ventricular myocardium by killing the animals at approximately 100 s after radiotracer injection. A hydraulic occluder stopped the flow to a portion of the myocardium supplied by the left circumflex coronary artery 30 s before tracer injection. Regional flows ranged from 0.1 to 7.0 ml.g-1.min-1. IPPA extractions ranged from 20 to 49%. Using the known flows, we assumed the applicability of an axially distributed blood-tissue exchange model to estimate the PS for the capillary (PSc) and the parenchymal cell. It was impossible to explain the data if the PSc values for membrane transport were uniform throughout the organ. Rather, the only reasonable descriptors of the data required that local PSc values increase with local flow, almost in proportion. Current methods of analysis using data based on deposition methods need to be revised to take into account the near proportionality of PS to flow for at least some substrates.
authors.affiliation: Division of Cardiology, Veterans Affairs Medical Center, Seattle, Washington 98108.
authors.forename: J H
authors.initials: JH
authors.lastname: Caldwell
authors.forename: G V
authors.initials: GV
authors.lastname: Martin
authors.forename: G M
authors.initials: GM
authors.lastname: Raymond
authors.forename: J B
authors.initials: JB
authors.lastname: Bassingthwaighte
mesh_terms: D000818:Animals; D002199:Capillary Permeability; D003326:Coronary Circulation; D004285:Dogs; D007462:Iodobenzenes; D008863:Microspheres; D008955:Models, Cardiovascular; D009043:Motor Activity
chemical_list: D007462:Iodobenzenes; C025391:omega-(4-iodophenyl)pentadecanoic acid