title: Cataloging of the genes expressed in human keratinocytes: analysis of 607 randomly isolated cDNA sequences.
abstract: The partial nucleotide sequences of 607 cDNAs randomly isolated from a cDNA library of cultured human epidermal keratinocytes were determined by single pass sequencing. Homology search of the sequences to the non-redundant nucleotide databases revealed that 27% of the cDNAs matched registered human-or non-human genes encoding not only keratinocyte specific genes, but also a variety of functional proteins, the expression of which had not been identified in keratinocytes. Non-matching cDNAs covering 49% of the cDNAs were not homologous even to ESTs from other organs, suggesting that these cDNAs include novel genes expressed in the cells. The large scale sequencing of keratinocyte cDNAs provides a useful molecular source for research into biology and diseases of the skin.
authors.affiliation: Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Konishi
authors.forename: Y
authors.initials: Y
authors.lastname: Morishima
authors.forename: E
authors.initials: E
authors.lastname: Ueda
authors.forename: Y
authors.initials: Y
authors.lastname: Kibe
authors.forename: K
authors.initials: K
authors.lastname: Nonomura
authors.forename: K
authors.initials: K
authors.lastname: Yamanishi
authors.forename: H
authors.initials: H
authors.lastname: Yasuno
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000032:analysis*; D016208:Databases, Factual; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary