title: The amino acid sequence and reactive site of a single-headed trypsin inhibitor from wheat endosperm.
abstract: The sequence of a trypsin inhibitor, isolated from wheat endosperm, is reported. The primary structure was obtained by automatic sequence analysis of the S-alkylated protein and of purified peptides derived from chemical cleavage by cyanogen bromide and digestion with Staphylococcus aureus V8 protease. This protein, named wheat trypsin inhibitor (WTI), which is comprised of a total of 71 amino acid residues, has 12 cysteines, all involved in disulfide bridges. The primary site of interaction (reactive site) with bovine trypsin has been identified as the dipeptide arginyl-methionyl at positions 19 and 20. WTI has a high degree of sequence identity with a number of serine proteinase inhibitors isolated from both cereal and leguminous plants. On the basis of the findings presented, this protein has been classified as a single-headed trypsin inhibitor of Bowman-Birk type.
authors.affiliation: Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Viterbo, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Poerio
authors.forename: C
authors.initials: C
authors.lastname: Caporale
authors.forename: L
authors.initials: L
authors.lastname: Carrano
authors.forename: C
authors.initials: C
authors.lastname: Caruso
authors.forename: F
authors.initials: F
authors.lastname: Vacca
authors.forename: V
authors.initials: V
authors.lastname: Buonocore
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000032:analysis; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D004220:Disulfides; D014361:Trypsin Inhibitors