Interleukin-6 (IL-6) production and cell growth of cultured human ameningiomas:-interactions with interleukin-1 beta (IL-1 beta) and interleukin-4 (IL-4) in vitro.
We examined the production of interleukin (IL)-6 by human meningioma cells in vitro, and the effects of IL-1 beta and IL-4 on IL-6 production and meningioma cell growth. The histological classification of the tumours studied included transitional, syncytial, fibroblastic and atypical. All 10 meningiomas studied produced IL-6 (range 0.22-7.6 ng/ml/10(6) cells/24 h). Separate addition of IL-1 beta or IL-4 to cultures increased IL-6 production up to ten fold, and two to three fold, respectively. Growth studies with IL-6 indicated that this cytokine significantly increased terminal cell density at a concentration greater than 1 ng/ml in 60% of the meningioma cultures studied. IL-1 beta caused a significant decrease in the terminal cell density in 25% of the meningioma cultures studied whereas IL-4 had a tendency to significantly inhibit growth in 16.6% of the cultures. These data suggest that IL-6 production by meningiomas can be modified by other cytokines and secondly, that IL-6, IL-1 beta and IL-4 can modify growth in vitro and may act as autocrine factors in vivo. By further determining the cytokine profiles within meningiomas and their effects, a better understanding of meningioma growth characteristics may be obtained.
Boyle-Walsh
E
E
Department of Clinical Chemistry, University of Liverpool, UK.
Hashim
I A
IA
Speirs
V
V
Fraser
W D
WD
White
M C
MC
D002455:Cell Division / Q000187:drug effects; D017077:Culture Media, Conditioned; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis*; D008577:Meningeal Neoplasms / Q000378:metabolism* / Q000503:physiopathology; D008579:Meningioma / Q000378:metabolism* / Q000503:physiopathology; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D007375:Interleukin-1; D015850:Interleukin-6; D015847:Interleukin-4