title: alpha-Tocopherol protects against radical-induced injury in cultured neurons.
abstract: The effect of alpha-tocopherol on neurons in protecting against radical-induced injuries caused by cumene hydroperoxide was assessed using a culture system. Structural and biochemical deteriorations in both clonal neuroblastoma cells and primary cultured neurons were induced by cumene hydroperoxide. These deteriorative changes were prevented prevented by pretreatment of alpha-tocopherol. Since simultaneous supplementation of alpha-tocopherol and cumene hydroperoxide had limited protective effects, prophylactic administration of alpha-tocopherol for risk patients with brain diseases concerned with damages induced by free radical is recommended to hamper the progression of neuronal impairment after attacks.
authors.affiliation: Department of Pathology, Shiga University of Medical Science, Otsu, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Amano
authors.forename: M
authors.initials: M
authors.lastname: Ohashi
authors.forename: M
authors.initials: M
authors.lastname: Kirihara
authors.forename: X H
authors.initials: XH
authors.lastname: Yang
authors.forename: F
authors.initials: F
authors.lastname: Hazama
mesh_terms: D000818:Animals; D001555:Benzene Derivatives / Q000633:toxicity; D002478:Cells, Cultured; D002999:Clone Cells; D005609:Free Radicals / Q000009:adverse effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D001555:Benzene Derivatives; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D007770:L-Lactate Dehydrogenase; C007164:cumene hydroperoxide