Effect of intraruminal propionic acid infusion on metabolism of mesenteric- and portal-drained viscera in growing steers fed a forage diet: I. Volatile fatty acids, glucose, and lactate.
This experiment investigated the effect of intraruminal infusion of propionic acid on ruminal VFA metabolism and the absorption of nutrients by the mesenteric- and portal-drained viscera of seven Friesian steers, average BW 127 kg, fed a dried grass-pellet diet. Each received by random allocation 0 (control), .5, or 1.0 mol of propionic acid/d for 7 d. Ruminal acetate and propionate irreversible loss rates and carbon exchange between VFA and CO2 were measured during continuous intraruminal infusions of 2-14C-acetic acid and 2-14C-propionic acid. Ruminal acetate irreversible loss rate was not affected by propionic acid infusion (overall mean 8.09, error mean square [EMS] 2.68 mol/d), whereas propionate irreversible loss increased incrementally with PA supply (3.22 vs 4.16, EMS .61 mol/d, for control and 1.0 mol of propionic acid/d, respectively, P = .09). Glucose irreversible loss rate was increased at the highest level of PA infusion (2.84, 2.83, and 3.22, EMS .06 mol/d, for control, .5, and 1.0 mol of propionic acid/d, respectively; P = .02 for control vs .5 + 1.0), although the proportion of glucose irreversible loss derived from propionate remained constant (.6). Net absorption into venous blood showed that propionate was extensively metabolized in the rumen wall and that the tissues of the small intestine utilized acetate. Utilization of glucose was reduced in portal tissues as a result of intraruminal infusion, and the data were used to derive a model of glucose and lactate interrelationships in gut tissues.
Seal
C J
CJ
Department of Biological and Nutritional Sciences, Faculty of Agriculture and Biological Sciences, University of Newcastle upon Tyne, U.K.
Parker
D S
DS
D000042:Absorption; D000818:Animals; D002339:Carotid Arteries; D002417:Cattle / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008297:Male; D008642:Mesenteric Veins; D011169:Portal Vein; D011422:Propionates / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D012417:Rumen / Q000378:metabolism; D014781:Viscera / Q000187:drug effects / Q000378:metabolism*
D005232:Fatty Acids, Volatile; D007773:Lactates; D011422:Propionates; D005947:Glucose; C029658:propionic acid