Application of biotin-streptavidin technology in developing a xanthine biosensor based on a self-assembled phospholipid membrane.
We have designed an amperometric xanthine minibiosensor based on a supported biotinylated phospholipid membrane. To this membrane streptavidin-modified xanthine oxidase was coupled. The fabricated biosensor corresponds in shape and size to a needle of 0.3 mm in diameter. The assay is based on the electrochemical detection of enzymatically generated hydrogen peroxide. The response to xanthine was linear up to 1 mmol.l-1 with a detection limit of 0.02 mmol.l-1. The response time was less than one minute and the biosensor was stable for at least five days.
Rehák
M
M
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji.
Snejdárková
M
M
Otto
M
M
D001426:Bacterial Proteins; D015374:Biosensing Techniques; D001710:Biotin; D008051:Lipid Bilayers; D010743:Phospholipids; D019809:Streptavidin; D019820:Xanthine; D014970:Xanthines / Q000032:analysis*
D001426:Bacterial Proteins; D008051:Lipid Bilayers; D010743:Phospholipids; D014970:Xanthines; D019820:Xanthine; D001710:Biotin; D019809:Streptavidin