Virucidal effect of myeloperoxidase on human immunodeficiency virus type 1-infected T cells.
Myeloperoxidase is virucidal to human immunodeficiency virus type 1 (HIV-1) in the persistently infected CEM human T-cell line or in acutely infected human peripheral blood mononuclear cells, as judged by viral infectivity and P24 radioimmunoassay. HIV-1 was specifically inactivated by low doses of the human myeloperoxidase (1.4 to 14.3 mU/ml) and the cells were spared. A higher enzyme concentration (143 mU/m) was cytotoxic, but uninfected CEM cells and normal lymphocytes were resistant to > or = 143 mU of myeloperoxidase per ml. The enzyme was virucidal with the Cl- present in medium and did not require exogenous H2O2. Catalase, an antioxidant enzyme, partially inhibited the virucidal effect of myeloperoxidase. Hence, the H2O2 probably came from the HIV-infected cells themselves. These in vitro findings indicate that the myeloperoxidase system is capable of inactivating HIV-1 of infected cells.
Chochola
J
J
Laboratoire d'Immunologie, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Yamaguchi
Y
Y
Moguilevsky
N
N
Bollen
A
A
Strosberg
A D
AD
Stanislawski
M
M
D000998:Antiviral Agents / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D002460:Cell Line; D005949:Glucose Oxidase / Q000494:pharmacology; D016655:HIV Core Protein p24 / Q000276:immunology / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007784:Lactoperoxidase / Q000494:pharmacology; D009195:Peroxidase / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000187:drug effects / Q000382:microbiology*
D000998:Antiviral Agents; D016655:HIV Core Protein p24; D010835:Phytohemagglutinins; D005949:Glucose Oxidase; D007784:Lactoperoxidase; D002374:Catalase; D009195:Peroxidase