Tn5401 disruption of the spo0F gene, identified by direct chromosomal sequencing, results in CryIIIA overproduction in Bacillus thuringiensis.
The Bacillus thuringiensis spo0F gene was identified by chromosomal DNA sequencing of sporulation mutants derived from a B. thuringiensis transposon insertion library. A spo0F defect in B. thuringiensis, which was suppressed by multicopy hknA or kinA, resulted in the overproduction of the CryIIIA insecticidal crystal protein.
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Ecogen Inc., Langhorne, Pennsylvania 19047-1810.
Baum
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D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D001427:Bacterial Toxins; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements / Q000235:genetics; D004731:Endotoxins / Q000096:biosynthesis*; D005798:Genes, Bacterial / Q000235:genetics*; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D014158:Transcription, Genetic
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D004731:Endotoxins; D006460:Hemolysin Proteins; C080951:Spo0F protein, Bacillus subtilis; C030956:insecticidal crystal protein, Bacillus Thuringiensis