Cloning and analysis of chicken acetylcholinesterase transcripts from muscle and brain.
We have isolated cDNA clones from an embryonic chicken muscle cDNA library which encodes the complete catalytic T subunit of acetylcholinesterase. The deduced polypeptide comprises 767 amino acids, shows approximately 60% homology to acetylcholinesterases from other vertebrates and contains a 155 amino acid sequence inserted into the middle of the peptide which is unique to the chicken enzyme. Northern blots of embryonic chicken muscle and adult brain show three transcripts approximately 4.5, 5.5, and 6.0 kb hybridizing to a cDNA fragment of AChE. The 6.0 kb transcript is expressed transiently in embryonic muscle and is a major transcript in adult brain.
Randall
W R
WR
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
Rimer
M
M
Gough
N R
NR
D000110:Acetylcholinesterase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014101:Torpedo; D014158:Transcription, Genetic; D014714:Vertebrates / Q000235:genetics
D007527:Isoenzymes; D011994:Recombinant Proteins; D000110:Acetylcholinesterase