Selective delivery of a greater amount of methotrexate to regional lymph nodes using a new dosage formulation, methotrexate adsorbed on activated carbon particles, in rats.
A new dosage formulation (MTX-CH) comprising methotrexate (MTX) adsorbed onto a suspension of activated carbon particles was developed for the treatment of lymph node metastases. In vitro studies showed that activated carbon particles adsorbed a great amount of MTX. Subcutaneous injection of MTX-CH into the left hind foot pad of rats distributed a greater amount of MTX selectively to the regional lymph nodes (the left popliteal node and the para-aortic nodes) for a longer period of time than similar administration of MTX aqueous solution with a smaller amount of MTX distributed to the rest of the whole body.
Hagiwara
A
A
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Takahashi
T
T
Sawai
K
K
Sakakura
C
C
Yamaguchi
M
M
Osaki
K
K
Tsujimoto
H
H
Ohyama
T
T
Tomoda
H
H
Yamamoto
A
A
D000327:Adsorption; D000818:Animals; D002244:Carbon; D002851:Chromatography, High Pressure Liquid; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008727:Methotrexate / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics; D010316:Particle Size; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013154:Spleen / Q000378:metabolism; D013535:Suspensions; D013816:Thermodynamics; D014018:Tissue Distribution
D013535:Suspensions; D002244:Carbon; D008727:Methotrexate