title: Purification of cardiac annexin V from the beagle dog heart and changes in its localization in the ischemic rat heart.
abstract: We isolated and purified 35 kDa protein from the myocardium of the beagle dog and identified it to be annexin V from partial amino acid sequence determination. It was confirmed that anticanine cardiac annexin V rabbit polyclonal antibody, which was produced using the 35 kDa protein, cross-reacts with annexin V of the myocardium, lung, liver, kidney, and brain of the rat. The localization of cardiac annexin V and the effect of ischemia for 30-180 min in the rat were immunohistochemically studied with the use of the Langendorff perfusion heart. In the normal myocardium, annexin V, accompanied by cross-striation, was observed throughout the cell. In ischemia of 30 min, extracellular leakage of annexin V was observed with uneven staining in the cytoplasm. When the ischemic time exceeded 60 min, annexin V was observed in the cell membrane with a decrease of annexin V in the cytoplasm. Also, extracellular leakage of annexin V was observed prominently. In ischemia for 180 min, almost all the annexin V in the cytoplasm disappeared. These results suggest that the level of ischemia can be estimated from the changes in localization of annexin V.
authors.affiliation: Department of Cardiology, Tokyo Women's Medical College, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kaneko
authors.forename: R
authors.initials: R
authors.lastname: Matsuda
authors.forename: F
authors.initials: F
authors.lastname: Chiwaki
authors.forename: S
authors.initials: S
authors.lastname: Hosoda
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000302:isolation & purification*; D000906:Antibodies; D015153:Blotting, Western; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D008297:Male; D017202:Myocardial Ischemia / Q000473:pathology*; D009206:Myocardium / Q000473:pathology*; D010477:Perfusion; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
chemical_list: D017304:Annexin A5; D000906:Antibodies