Cell lines used for the selection of recombinant baculovirus.
Four insect cell lines were used to isolate two recombinant baculoviruses which had the beta-galactosidase (beta-gal) gene for colorimetric assay purposes. Plaque assays were performed using two Trichoplusia ni cell lines: BTI-TN-5B1-4 and TN-368, and two Spodptera frugiperda cell lines: IPLB-SF-21AE and SF9. The number of plaques (occlusion positive and blue beta-gal+ recombinants) formed in the Trichoplusia cells was higher than in the Spodoptera cells. The appearance of Autographa californica NPV polyhedra was also faster in the T. ni cell lines. The effect of cell passage on the plaque formation proved to be critical when two different passages of the SF9 cells were tested. The higher passage produced a lower viral titration. The size and time of appearance of the plaques was also different.
Maruniak
J E
JE
Entomology and Nematology Department, University of Florida, Gainesville 32611.
Garcia-Canedo
A
A
Rodrigues
J J
JJ
D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000302:isolation & purification; D002460:Cell Line; D004247:DNA / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D009036:Moths / Q000166:cytology* / Q000382:microbiology; D011995:Recombination, Genetic; D013997:Time Factors; D010948:Viral Plaque Assay; D001616:beta-Galactosidase / Q000235:genetics
D004247:DNA; D001616:beta-Galactosidase