Specific interaction of penicillin-binding proteins 3 and 7/8 with soluble lytic transglycosylase in Escherichia coli.
Soluble lytic transglycosylase 70 (Slt70), one of the better characterized murein hydrolases of Escherichia coli, was covalently bound to CNBr-activated Sepharose and used as a specific tool to screen for proteins showing an affinity for Slt70. Several proteins were specifically enriched by Slt-Sepharose affinity chromatography. Two of them were identified as the penicillin-binding proteins (PBP)3 and PBP7/8. Thus, the bifunctional synthase PBP3, specifically involved in septum formation, and PBP7/8, recently shown to be a DD-endopeptidase, bind to Slt70 in vitro. In addition, PBP7/8 was found not only to stabilize but also to stimulate the enzymatic activity of Slt70 by a protein-protein interaction. It is concluded that Slt70, PBP7/8, and PBP3 may form a multienzyme complex in vivo.
Romeis
T
T
Max-Planck-Institut für Entwicklungsbiologie, Abteilung Biochemie, Tübingen, Federal Republic of Germany.
Höltje
J V
JV
D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D016695:Glycosyltransferases; D006602:Hexosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D009097:Multienzyme Complexes / Q000302:isolation & purification / Q000378:metabolism*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000302:isolation & purification / Q000378:metabolism*; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000378:metabolism; D010457:Peptidoglycan / Q000378:metabolism; D010458:Peptidyl Transferases / Q000302:isolation & purification / Q000378:metabolism*; D014166:Transferases / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D010457:Peptidoglycan; D014166:Transferases; D010458:Peptidyl Transferases; D016695:Glycosyltransferases; D006602:Hexosyltransferases; C021815:murein transglycosylase; D002267:Muramoylpentapeptide Carboxypeptidase