title: Glucose and insulin responses in isolated human lymphocytes reflect in vivo status: effects of VLCD treatment.
abstract: Human lymphocyte growth response to mitogen was examined in vitro under different conditions to monitor aspects of carbohydrate utilization in three groups: obese nondiabetic, obese/NIDDM, and normal-weight individuals. Although lymphocyte growth capacity in minimal medium for these three groups was found to be similar at a glucose concentration of 72 micrograms/ml in the absence of insulin or glycerol, differences in mitogen-stimulated growth were observed at lower glucose concentrations or in the presence of insulin or glycerol. Interestingly, these metabolic alterations in lymphocytes can be normalized by treating obese and obese/NIDDM subjects with a very low calorie diet (VLCD) regimen. The results of this study indicate that lymphocyte culture in a defined medium may provide a mechanism to examine different metabolic states and to evaluate treatment regimens (diet, exercise, etc.) for obese and NIDDM subjects.
authors.affiliation: Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251.
authors.forename: K Y
authors.initials: KY
authors.lastname: Tu
authors.forename: R
authors.initials: R
authors.lastname: Matthews
authors.forename: N H
authors.initials: NH
authors.lastname: Topek
authors.forename: K S
authors.initials: KS
authors.lastname: Matthews
mesh_terms: D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003920:Diabetes Mellitus / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D002149:Energy Intake; D005947:Glucose / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D009765:Obesity / Q000097:blood*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D005947:Glucose; D005990:Glycerol