Selective isolation of poliovirus in recombinant murine cell line expressing the human poliovirus receptor gene.
Sixty-eight laboratory strains representing 49 enterovirus, 10 adenovirus, and 3 reovirus serotypes were inoculated in a recombinant murine cell line expressing the human poliovirus receptor gene (L alpha cells). Only polioviruses caused cytopathic effect over a 10-day period. Likewise, only polioviruses were isolated, by use of L alpha cells, from 168 fecal specimens from children from developing countries. These results suggest that the recombinant L alpha cells can be used for selective isolation of poliovirus from clinical specimens.
Hovi
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Enterovirus Laboratory, National Public Health Institute (KTL), Helsinki, Finland.
Stenvik
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D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005069:Evaluation Studies as Topic; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D011051:Poliomyelitis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D017955:Poliovirus / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology; D011055:Poliovirus Vaccine, Oral; D011991:Receptors, Virus / Q000235:genetics*; D014773:Virology / Q000379:methods; D014779:Virus Replication
D008565:Membrane Proteins; D011055:Poliovirus Vaccine, Oral; D011991:Receptors, Virus; C066100:poliovirus receptor