title: Comparison of immunohistochemistry, acid-fast staining, and cultivation for detection of Mycobacterium paratuberculosis in goats.
abstract: Forty-seven paired specimens of ileum and mesenterial lymph nodes from goats originating from 2 herds with paratuberculosis were investigated. Culture of the specimens for Mycobacterium paratuberculosis was compared with Ziehl-Neelsen staining and with immunohistochemical tests on paraffin-embedded tissue sections, using a M. paratuberculosis immune serum and an avidin-biotin-alkaline phosphatase method. Immunohistochemical techniques detected most positive samples and produced more clearly visible reactions than did acid-fast staining. Eighteen of 47 samples were positive by immunohistochemical techniques may represent a valuable adjunct to standard techniques for diagnosis of paratuberculosis in goats.
authors.affiliation: Central Veterinary Laboratory, Oslo, Norway.
authors.forename: O F
authors.initials: OF
authors.lastname: Thoresen
authors.forename: K
authors.initials: K
authors.lastname: Falk
authors.forename: O
authors.initials: O
authors.lastname: Evensen
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D015511:Goat Diseases / Q000175:diagnosis* / Q000382:microbiology; D006041:Goats; D006651:Histocytochemistry / Q000379:methods; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008828:Microbiological Techniques / Q000662:veterinary; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial