Evidence for nitric oxide-mediated oxidative damage in chronic inflammation. Nitrotyrosine in serum and synovial fluid from rheumatoid patients.
Reaction of nitric oxide (NO.) with superoxide radical generates peroxy-nitrite, which can decompose to products that nitrate aromatic amino acids. Such nitro-aromatics may be 'markers' of NO.-dependent oxidative damage. Blood serum and synovial fluid from patients with the inflammatory joint disease rheumatoid arthritis contain 3-nitrotyrosine. By contrast, body fluids from normal subjects and patients with osteoarthritis contain no detectable 3-nitrotyrosine; much lower levels were found in serum from patients in the early stages of rheumatoid arthritis. This is evidence that NO. plays a role in joint damage in rheumatoid arthritis.
Kaur
H
H
Pharmacology Group, King's College, London, UK.
Halliwell
B
B
D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013582:Synovial Fluid / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism
D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine