Use of [6,6-2H2]glucose and of low-enrichment [U-13C6]-glucose for sequential or simultaneous measurements of glucose turnover by gas chromatography-mass spectrometry.
We developed gas chromatography-mass spectrometric methods for assaying the enrichment of 99 at.% [6,6-2H2]glucose and 30 at.% [U-13C6]glucose, although both tracers are mostly M + 2. 13C enrichment is determined either by the C-1 to C-5 fragment of glucose aldonitrile pentaacetate or by oxidation of glucose to glucarate. 2H enrichment is assayed as the difference between the 13C enrichment of glucarate and the 2H + 13C enrichment of glucose. The techniques, which were validated in in vivo experiments, are applicable to the determination of simultaneous or sequential measurements of the rate of glucose appearance before and after an intervention. They could also be applied to the simultaneous determination of (i) gluconeogenesis by incorporation of a 13C-labeled precursor into glucose and (ii) the rate of glucose appearance by [6,6-2H2]glucose infusion.
Previs
S F
SF
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106.
Ciraolo
S T
ST
Fernandez
C A
CA
Beylot
M
M
Agarwal
K C
KC
Soloviev
M V
MV
Brunengraber
H
H
D000818:Animals; D002247:Carbon Isotopes; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D005937:Glucaric Acid / Q000737:chemistry*; D005947:Glucose / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D009570:Nitriles / Q000032:analysis*; D010084:Oxidation-Reduction; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley
D002247:Carbon Isotopes; D009570:Nitriles; C088693:glucose aldonitrile pentaacetate; D003903:Deuterium; D005947:Glucose; D005937:Glucaric Acid