tRNA regions which contact with the ribosomal poly(U)-programmed P-site.
Equilibrium binding affinity of yeast tRNA(Phe) for Escherichia coli poly(U)-programmed 70S ribosomal P-site was compared with corresponding affinities of several tRNA(Phe) 3'- and 5'-end-truncated derivatives, all containing the anticodon arm. Our findings strongly suggest that besides three 3'-terminal-CCA nucleotides (C74, C75 and A76), only the tRNA(Phe) anticodon arm (N28-N42) contains ribosomal P-site contact centers and that there are no such centers in the intermediate regions N1-N27 and N43-N73.
Nekhai
S A
SA
Division of Radiation and Molecular Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad region.
Parfenov
D V
DV
Saminsky
E M
EM
D000926:Anticodon; D001483:Base Sequence; D001665:Binding Sites; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000737:chemistry; D011072:Poly U / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D000926:Anticodon; D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D012360:RNA, Transfer, Phe; D011072:Poly U