A simple method for N-15 labelling of exocyclic amino groups in synthetic oligodeoxynucleotides.
The use of the ammonia deprotection step to introduce (15)N labels at specific exocyclic amino positions of adenine, cytosine, guanine or 2-aminopurine of oligodeoxynucleotides is described.
Acedo
M
M
Department of Molecular Genetics, CID-CSIC, Barcelona, Spain.
Fàbrega
C
C
Aviño
A
A
Goodman
M
M
Fagan
P
P
Wemmer
D
D
Eritja
R
R
D015075:2-Aminopurine; D000225:Adenine; D000641:Ammonia; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003596:Cytosine; D006147:Guanine; D007553:Isotope Labeling / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis
D009587:Nitrogen Isotopes; D009838:Oligodeoxyribonucleotides; D015075:2-Aminopurine; D006147:Guanine; D000641:Ammonia; D003596:Cytosine; D000225:Adenine