title: A microbiological assay for determining sarafloxacin and oxolinic acid concentrations in Atlantic salmon plasma.
abstract: A microbiological assay was developed to quantify the concentrations of the quinolone antibiotics sarafloxacin and oxolinic acid in Atlantic salmon (Salmo salar) plasma. The assay was a modification of the Association of Official Analytical Chemists (AOAC) method for antibiotic concentrations in feeds using disk diffusion and a lawn of Vibrio anguillarum ATCC 19264 as the test organism. With these modifications, sensitivities of 0.04 microgram/mL for sarafloxacin and 0.125 micrograms/mL for oxolinic acid were obtained.
authors.affiliation: Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Exward Island, Charlottetown, Canada.
authors.forename: J S
authors.initials: JS
authors.lastname: Giles
authors.forename: H
authors.initials: H
authors.lastname: Hariharan
authors.forename: S B
authors.initials: SB
authors.lastname: Heaney
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents; D001412:Bacillus subtilis; D001681:Biological Assay; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000097:blood; D004926:Escherichia coli; D024841:Fluoroquinolones; D010093:Oxolinic Acid / Q000097:blood*; D012474:Salmon; D014733:Vibrio
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D002939:Ciprofloxacin; D010093:Oxolinic Acid; C047224:sarafloxacin