title: Differentiation of Cryptococcus neoformans serotypes A and D using creatinine dextrose bromothymol blue thymine medium.
abstract: A serotype differentiation of the encapsulated yeast Cryptococcus neoformans is described using creatinine dextrose bromothymol blue thymine (CDBT) medium. On CDBT medium C. neoformans serotype D grew as bright red colonies, turning the medium a bright orange after five days incubation at 28 degrees C. C. neoformans serotype A grew as pale colonies with no apparent colour effect on the medium. Serotypes B and C caused a slight greening of the medium. The reaction of the four serotypes of C. neoformans on CDBT medium is considered useful in the differentiation of the closely related serotype A and D.
authors.affiliation: National Veterinary Research Institute, Vom, Nigeria.
authors.forename: E A
authors.initials: EA
authors.lastname: Irokanulo
authors.forename: C O
authors.initials: CO
authors.lastname: Akueshi
authors.forename: A A
authors.initials: AA
authors.lastname: Makinde
mesh_terms: D001979:Bromthymol Blue; D003404:Creatinine; D003455:Cryptococcus neoformans / Q000145:classification*; D003470:Culture Media; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D012703:Serotyping / Q000379:methods*; D013941:Thymine
chemical_list: D003470:Culture Media; D003404:Creatinine; D005947:Glucose; D013941:Thymine; D001979:Bromthymol Blue