title: Stimulation of ileal calcium absorption by sorbitol, L-xylose, or creatine via a decrease in luminal sodium concentration: relation with concomitant changes in enterocyte energy metabolism.
abstract: Ligated ileal loops, 30 cm in length, of 4-month-old male Wistar rats were instilled with 3 ml of a 10 mM CaCl2 solution (added with 0.25 muCi 45Ca) in the absence (control) or presence of 100mM sorbitol, L-xylose, or creatine. Ileal calcium (Ca) transport, measured by plasma 45Ca appearance, was found to be similar 30 minutes after fluid instillation in all four instances. However, thereafter, 45Ca appearance in plasma did not increase further in control animals whereas it increased twice as much during the subsequent 30 minutes in the presence of sorbitol, L-xylose, or creatine. However, when loops of similar length were instilled with only 1.0 ml of such solutions, the sorbitol effect was already observed during the first 30 minutes. The stimulation of ileal Ca absorption induced by the presence of sorbitol appeared to be due to a cellular effect, associated with a decreased flux across the paracellular pathway, as indicated by 3H-mannitol absorption. The presence of sorbitol in instilled ileal solution induced a significant decrease in luminal Na, K, bicarbonate, and Cl concentrations at each time point studied (30, 60, 120, or 240 minutes after instillation). Thirty minutes after instillation, no difference in soluble Ca concentration was observed between control and experimental rats. After 60 minutes, Ca concentration was dramatically decreased in control rats but it remained nearly constant in experimental animals. Thus, the presence of substances enhancing ileal Ca transport favored the maintenance of soluble Ca in ileal solution during longer time periods than their absence. In the ileal enterocyte, these substances induced a twofold increase of ATP content compared with controls.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratoire EPHE, Métabolisme Minéral des Mammifères, Physiologie, Faculté de Pharmacie, Chatenay-Malabry, France.
authors.forename: S
authors.initials: S
authors.lastname: Tardivel
authors.forename: P
authors.initials: P
authors.lastname: Fournier
authors.forename: Y
authors.initials: Y
authors.lastname: Dupuis
authors.forename: B
authors.initials: B
authors.lastname: Nabarra
authors.forename: T
authors.initials: T
authors.lastname: Drueke
authors.forename: B
authors.initials: B
authors.lastname: Lacour
mesh_terms: D000042:Absorption / Q000187:drug effects; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002713:Chlorine / Q000378:metabolism; D003401:Creatine / Q000494:pharmacology*; D004734:Energy Metabolism; D007082:Ileum / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008353:Mannitol / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000648:ultrastructure; D010710:Phosphates / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D013012:Sorbitol / Q000494:pharmacology*; D014994:Xylose / Q000494:pharmacology*
chemical_list: D001639:Bicarbonates; D010710:Phosphates; D008353:Mannitol; D002713:Chlorine; D013012:Sorbitol; D000255:Adenosine Triphosphate; D012964:Sodium; D014994:Xylose; D003401:Creatine; D011188:Potassium; D002118:Calcium