Cell type specific patterns of mRNA splicing in hepatoma cells transfected with the mutated albumin minigene of Nagase analbuminemic rats.
We constructed an SV40-derived expression vector containing a mutated albumin minigene of Nagase analbuminemic rats (NAR), and introduced it into cultured cells. Transient expression of the minigene mRNA was determined by RT-PCR. Three kinds of aberrant mRNAs were expressed by the non-hepatic cells COS-1, and the undifferentiated human hepatoma cells HLE, transfected with the minigene. Their predominant mRNA lacked exon H (delta H), while mRNAs lacking exons H and I, or exons G and H were less abundant. This pattern of the mRNAs was similar to that of albumin mRNAs in the liver of old NAR. In contrast, a differentiated type of human hepatoma cell line, HepG2, expressed only delta H mRNA, like young NAR. These findings indicate that the expression system of the mutated minigene in cultured hepatoma cells is useful for understanding two-exon-skipping of albumin pre-mRNA of NAR.
Sugiyama
K
K
Investigative Cancer Treatment Division, National Cancer Center Research Institute, Chiba, Japan.
Hitomi
Y
Y
Adachi
H
H
Esumi
H
H
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D017931:DNA Primers / Q000737:chemistry; D005091:Exons; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011922:Rats, Mutant Strains / Q000235:genetics*; D012709:Serum Albumin / Q000235:genetics*; D014162:Transfection
D017931:DNA Primers; D012333:RNA, Messenger; D012709:Serum Albumin