Characterization of glucose transport and cloning of a hexose transporter gene in Trypanosoma cruzi.
A gene from Trypanosoma cruzi, TcrHT1, which encodes a member of the glucose transporter superfamily has been cloned. The gene is similar in sequence to the T. brucei hexose transporter THT1 and the Leishmania transporter Pro-1 and is present in the T. cruzi genome as a cluster of at least eight tandemly reiterated copies. Northern blot analysis revealed two mRNA transcripts which differ in size with respect to their 3' untranslated regions. When injected with in vitro transcribed TcrHT1 mRNA, Xenopus oocytes express a hexose transporter with properties similar to those of T. cruzi. Glucose transport in T. cruzi is mediated via a carrier with unique properties when compared with the other glucose transporters already characterized among the Kinetoplastida. It is a facilitated transporter with a high affinity for D-glucose (Km = 84.1 +/- 7.9 microM and Vmax = 46 +/- 9.4 nmol/min per mg of protein) that shares with other kinetoplastid hexose transporters the ability to recognize D-fructose, which distinguishes these carriers from the human erythrocyte glucose transporter GLUT1.
Tetaud
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Laboratoire Biologie Moleculaire et Immunologie de Protozoaires Parasites, Université Bordeaux II, France.
Bringaud
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F
Chabas
S
S
Barrett
M P
MP
Baltz
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001693:Biological Transport, Active / Q000187:drug effects; D015152:Blotting, Northern; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D017125:Genes, Protozoan; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D007891:Leishmania / Q000378:metabolism; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009005:Monosaccharides / Q000494:pharmacology; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016053:RNA, Protozoan / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014349:Trypanosoma cruzi / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; D018076:DNA, Complementary; D009004:Monosaccharide Transport Proteins; D009005:Monosaccharides; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D011994:Recombinant Proteins; C079374:THT1 protein, Trypanosoma brucei; C100828:TcrHT1 protein, Trypanosoma cruzi; D005947:Glucose