Mucoadhesion of latexes. I. Analytical methods and kinetic studies.
A Fourier transform infrared spectroscopy/attenuated total reflection technique for direct quantification of adsorbed poly(styrene) latexes on rat intestinal mucosa was developed for deposited latex amounts up to 1.5 g/m2. The method agreed well with another dosage assay of adsorbed particles by turbidimetry after denaturation of the mucus. Adsorption kinetics were made under static conditions at latex concentrations of 4 g/L in physiological saline. Ninety percent of equilibrium was reached after 10 min for a particle size of 230 nm, 20 min for a size of 320 nm, and 30 min for a size of 670 nm. The plateaus were between 0.6 and 0.9 g/m2 (adsorbed mass per apparent surface of mucosa). The first phase of the kinetics was theoretically approached by a diffusion model in the suspension medium. Mucosa from rat jejunum and ileum could be considered as a homogeneous biological model for latex adsorption.
Durrer
C
C
Centre d'Etudes Pharmaceutique, URA CNRS 1218, Université Paris-Sud, Châtenay-Malabry, France.
Irache
J M
JM
Puisieux
F
F
Duchêne
D
D
Ponchel
G
G
D000327:Adsorption; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D007700:Kinetics; D007840:Latex / Q000737:chemistry*; D008297:Male; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry; D010316:Particle Size; D011137:Polystyrenes; D051381:Rats; D017208:Rats, Wistar; D013816:Thermodynamics
D007840:Latex; D011137:Polystyrenes