[Sensitivity of immunocompetent cells to a hormonal signal in the presence of mitogen].
The chorionic gonadotropin (Cg) effects on the ability of intact and mitogen-activated splenocytes to form the adoptive immune response have been studied in a short-term cell culture. It has been established, that an hour incubation of non-fractionated spleen cells with CG (40 or 200 IU) leads to plaque-forming cell (PFC) suppression. The culture, deprived of macrophages, does not react on the hormone depressive effects, but its low dose, on the contrary, stimulates the PFC formation. Lipopolysaccharide addition into the culture of non-fractionated splenocytes does not influence the PFC level, while the CG addition leads to intensification of PFC formation processes. In the cell culture, deprived of macrophages, hormone abolishes the immunostimulating effect of lipopolysaccharide. The simultaneous addition of non-fractionated CG splenocytes and monoclonal anti-IgM antibodies does not influence the immunostimulating effect of mitogenic antibodies. The possible mechanisms of CG effect and the problem of hormonal immunocompetent cell regulation, depending on their functional activity are under consideration.
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D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008934:Mitogens / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D013154:Spleen / Q000166:cytology / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D006063:Chorionic Gonadotropin; D008070:Lipopolysaccharides; D008934:Mitogens
Chuvstvitel'nost' immunokompetentnykh kletok k gormonal'nomu signalu na fone mitogennogo vozdeĭstviia.