Cloning and expression of bovine and porcine interleukin-2 in baculovirus and analysis of species cross-reactivity.
The cDNAs encoding bovine and porcine interleukin-2 (IL-2) have been expressed using the baculovirus Autographa californica nuclear polyhedrosis virus as a vector in insect cells. Insect cells infected with recombinant viruses secreted bovine and porcine IL-2 into the culture medium, with biological activities for maintaining the proliferation of homologous cells. When the activities of these two IL-2 proteins and commercially available human IL-2 were tested on heterologous cells differences were found. Recombinant bovine (rb)IL-2 only supported the growth of bovine lymphocytes and was not active on human, mouse or porcine lymphocytes. Recombinant porcine (rp)IL-2 and recombinant human (rh)IL-2 supported the proliferation of human, bovine, porcine and murine cells. However, the proliferative response of human lymphocytes to rpIL-2 was only 50% of that seen with rhIL-2. Sequence differences at the predicted p55 and p75 contact binding sites may explain this.
Collins
R A
RA
Division of Immunology and Pathology, Institute for Animal Health, Compton, Newbury, UK.
Tayton
H K
HK
Gelder
K I
KI
Britton
P
P
Oldham
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000276:immunology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003429:Cross Reactions; D017931:DNA Primers; D015870:Gene Expression; D006801:Humans; D007313:Insecta / Q000382:microbiology; D007376:Interleukin-2 / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013552:Swine / Q000276:immunology*
D017931:DNA Primers; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins