[Cloning and sequencing of a DNA fragment from Plasmodium falciparum FCC1/HN isolate].
A specific DNA fragment isolated from Plasmodium falciparum FCC1/HN isolate has been cloned in Bam H1 site of pUC18 and first partially sequenced by Sanger's method. The results show: G+C percent of DNA sequence is 26.8%, and the cloned DNA has many restriction sites which are conventional for subcloning. The authors suggest that this clone and sequence may be used as a guide for developing a DNA probe or PCR primers.
Yu
Y
Y
Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, Shanghai.
He
J W
JW
Zheng
Z X
ZX
Guan
W B
WB
Zhou
Y C
YC
Cai
L R
LR
Ye
F Y
FY
Xin
H
H
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010963:Plasmodium falciparum / Q000235:genetics*; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D010446:Peptide Fragments