Sertoli cell secretion of a factor that inhibits the incorporation of 3H-thymidine into cells in vitro.
Rat Sertoli cells secrete a low molecular weight factor in vitro that inhibits the incorporation of 3H-thymidine into cells. Although the addition of Sertoli cell-conditioned medium (rSCCM) resulted in nearly a threefold stimulation of cell growth, the incorporation of 3H-thymidine was decreased in a dose-dependent manner and did not reflect the increase in cell number. Peritubular cell- and germ cell-conditioned medium did not contain this inhibitory activity. Nor did the conditioned medium from fibroblasts and a variety of cell lines tested. A low molecular weight filtrate of rSCCM (< 1,000 Da) contained virtually all of the 3H-thymidine inhibiting activity, as well as about 50% of the mitogenic activity in the rSCCM. The inhibitory activity was eliminated upon removal of the rSCCM and was not due to either growth inhibition or a toxic effect on cell proliferation.
Lamb
D J
DJ
Scott Department of Urology, Baylor College of Medicine, Houston, Texas 77030.
Lee
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S
Shubhada
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D000818:Animals; D001685:Biological Factors / Q000378:metabolism*; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D017077:Culture Media, Conditioned; D008297:Male; D009928:Organ Specificity; D051381:Rats; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013737:Testis / Q000166:cytology; D013936:Thymidine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014316:Tritium
D001685:Biological Factors; D003470:Culture Media; D017077:Culture Media, Conditioned; D014316:Tritium; D013936:Thymidine