The mixed leukocyte reaction may promote the growth of human pericorneal fibroblasts in primary culture.
Keratitis of the cornea is believed to be the leading cause of loss of vision due to external eye disease in the United States and in Europe. At present, primary and secondary cell cultures obtained from corneal explants are the most suitable system to study the role of drug-resistant viruses in severe recurrences of infections leading to irreversible corneal scarring. In this work, the growth of human pericorneal fibroblasts in primary and secondary cultures has been obtained by cocultivation with a mixed leukocyte reaction.
Muratore
O
O
Institute of Microbiology, University of Genoa, Italy.
Tonoli
E L
EL
Pesce Schito
A
A
Schito
G C
GC
Saccà
S C
SC
Ghiglione
D
D
Ciurlo
G
G
D002455:Cell Division; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*; D007959:Lymphocyte Culture Test, Mixed