Murine skin as a target for melatonin bioregulation.
Specific binding sites for 3H-melatonin were detected in membrane fractions prepared from C57 BL-6 mouse skin, and were localized to the epidermis and the epithelial bulb of the hair follicle by in situ autoradiography. In skin organ culture, melatonin stimulated DNA synthesis by the epidermal keratinocytes at concentration 0.1-10 nM, while at > or = 1 microM it inhibited tyrosinase activity. We concluded that murine skin is a target for melatonin bioregulation.
Slominski
A
A
Department of Pathology and Laboratory Medicine, Albany Medical College, NY 12208.
Chassalevris
N
N
Mazurkiewicz
J
J
Maurer
M
M
Paus
R
R
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006197:Hair / Q000254:growth & development; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis; D008550:Melatonin / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D008543:Melanins; D004247:DNA; D014442:Monophenol Monooxygenase; D008550:Melatonin