title: Structural characterization of PNA-DNA duplexes by NMR. Evidence for DNA in a B-like conformation.
abstract: The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by 1H NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.
authors.affiliation: Department of Biophysics, Arrhenius Laboratory, Stockholm University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Leijon
authors.forename: A
authors.initials: A
authors.lastname: Gräslund
authors.forename: P E
authors.initials: PE
authors.lastname: Nielsen
authors.forename: O
authors.initials: O
authors.lastname: Buchardt
authors.forename: B
authors.initials: B
authors.lastname: Nordén
authors.forename: S M
authors.initials: SM
authors.lastname: Kristensen
authors.forename: M
authors.initials: M
authors.lastname: Eriksson
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D011522:Protons
chemical_list: D009696:Nucleic Acids; D011522:Protons; D004247:DNA