Structural characterization of PNA-DNA duplexes by NMR. Evidence for DNA in a B-like conformation.
The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by 1H NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.
Leijon
M
M
Department of Biophysics, Arrhenius Laboratory, Stockholm University, Sweden.
Gräslund
A
A
Nielsen
P E
PE
Buchardt
O
O
Nordén
B
B
Kristensen
S M
SM
Eriksson
M
M
D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D011522:Protons
D009696:Nucleic Acids; D011522:Protons; D004247:DNA