title: Cryopreservation of human hookworms.
abstract: First-stage larvae (L1) of the human hookworms Ancylostoma duodenale and Necator americanus were frozen over liquid nitrogen after a 1-hr incubation in a cryoprotectant (10% DMSO and 10% dextran). Thawed larvae developed to the infective third stage (L3) on agar plates. In the case of A. duodenale, the larvae were infective to dogs. The infectivity of N. americanus L3 was not tested.
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
authors.forename: T J
authors.initials: TJ
authors.lastname: Nolan
authors.forename: V M
authors.initials: VM
authors.lastname: Bhopale
authors.forename: Z
authors.initials: Z
authors.lastname: Megyeri
authors.forename: G A
authors.initials: GA
authors.lastname: Schad
mesh_terms: D000722:Ancylostoma / Q000472:pathogenicity / Q000502:physiology*; D000724:Ancylostomiasis / Q000469:parasitology; D000818:Animals; D015925:Cryopreservation; D004285:Dogs; D006801:Humans; D007814:Larva / Q000472:pathogenicity / Q000502:physiology; D017221:Necator americanus / Q000472:pathogenicity / Q000502:physiology*; D009332:Necatoriasis / Q000469:parasitology