3H-naloxone binding in brain regions of normotensive Wistar, spontaneously hypertensive and renal hypertensive rats.
In order to study the role of opioid receptors in two models of experimental hypertension the binding of 3H-naloxone to membranes prepared from discrete brain regions and spinal cord was determined. Renal hypertensive rats (RHR) were found to have a greater density of 3H-naloxone binding sites in the hippocampus and hypothalamus when compared to spontaneously hypertensive rats (SHR) and normotensive Wistar rats (NR). The apparent dissociation constant (Kd) for 3H-naloxone binding did not differ between groups.
Widy-Tyszkiewicz
E
E
Department of Experimental and Clinical Pharmacology, Medical Academy, Warsaw, Poland.
Kotapski
J
J
Czlonkowski
A
A
D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000378:metabolism*; D006973:Hypertension / Q000378:metabolism*; D006977:Hypertension, Renal / Q000378:metabolism*; D008297:Male; D009270:Naloxone / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D012016:Reference Values; D014018:Tissue Distribution; D014316:Tritium
D014316:Tritium; D009270:Naloxone