title: Immunoelectron microscopic studies on the specific adhesion of Trypanosoma congolense to cultured vascular endothelial cells.
abstract: Bloodstream forms of Trypanosoma congolense were cocultivated in vitro with vascular endothelial cells. The trypanosomes adhere specifically to the endothelial surfaces of the anterior part of their flagella, as shown by scanning and transmission electron microscopy. The interaction between parasite and host cell is very tight, and frequently the accumulation of endocytotic vesicles near the contact site is observed. Immunoelectron microscopy revealed a compound distributed over the total surface of the trypanosomes and reacting with antibodies against the beta 1 integrin chain, but no reaction was found with anti-alpha 1 or anti-alpha 2 antibodies. Integrins are typical adhesion molecules and are now shown to be present at the surface of T. congolense by electron microscopy and by immunofluorescence. A direct participation of this substance in the specific adhesion to endothelium, however, could not be proven.
authors.affiliation: Institut für Anatomie, Freie Universität Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Shakibaei
authors.forename: M
authors.initials: M
authors.lastname: Milaninezhad
authors.forename: H J
authors.initials: HJ
authors.lastname: Risse
mesh_terms: D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002417:Cattle; D002448:Cell Adhesion; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000502:physiology* / Q000648:ultrastructure*; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D016253:Microscopy, Immunoelectron / Q000379:methods; D013997:Time Factors; D014348:Trypanosoma congolense / Q000502:physiology* / Q000648:ultrastructure*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000032:analysis
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D014643:Variant Surface Glycoproteins, Trypanosoma; D016650:Fluorescein-5-isothiocyanate