title: Histologic and ultrastructural changes after large-colon torsion, with and without use of a specific platelet-activating factor antagonist (WEB 2086), in ponies.
abstract: The role of platelet-activating factor (PAF) in mediating the colonic damage that develops after large-colon torsion was studied in 14 ponies. Morphologic changes in areas of the ascending colon and selected abdominal and thoracic viscera after 1 hour of large-colon torsion and 3 to 5 hours of reperfusion were determined, as well as the protective effects of systemic administration of a specific PAF antagonist (WEB 2086). Ponies were selected then allocated at random and in equal numbers to 2 groups that received 1 of 2 treatments prior to induction of large-colon torsion: group 1--control (saline solution), and group 2--WEB 2086 (3 mg/kg of body weight loading dose and 3 mg/kg/h for the remainder of the study). In each pony, full-thickness tissue specimens from the gastrointestinal tract--cecum, pelvic flexure, left and right ventral colon, and right dorsal colon--heart, left lung, liver, left adrenal gland, spleen, and right kidney were collected and histologically evaluated. Edema, mucosal necrosis, and neutrophil infiltration in colonic sections were graded from 0 (normal) to 3 (most severe changes). Sections of liver and lung from 3 ponies in each group, and colon from 1 pony in each group, also were examined by transmission electron microscopy to determine the presence of ultrastructural alterations. Ischemia and reperfusion induced marked changes in all sections of colon in all ponies: moderate to severe submucosal edema, moderate necrosis of the superficial epithelium and lamina propria, and necrosis of the mucosal crypt epithelium. Extravascular neutrophil accumulation was evident in all sections of colon and cecum, but not in other tissues.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824.
authors.forename: D V
authors.initials: DV
authors.lastname: Wilson
authors.forename: J S
authors.initials: JS
authors.lastname: Patterson
authors.forename: J A
authors.initials: JA
authors.lastname: Stick
authors.forename: P J
authors.initials: PJ
authors.lastname: Provost
mesh_terms: D000818:Animals; D001381:Azepines / Q000627:therapeutic use*; D003106:Colon / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D003108:Colonic Diseases / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D004487:Edema; D004848:Epithelium / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D006734:Horse Diseases; D006736:Horses; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009336:Necrosis; D009504:Neutrophils / Q000473:pathology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D014102:Torsion Abnormality; D014230:Triazoles / Q000627:therapeutic use*
chemical_list: D001381:Azepines; D010972:Platelet Activating Factor; D014230:Triazoles; C052518:WEB 2086