Serum levels of steroid sulfatase protein in gynecologic carcinomas.
Steroid sulfatase (STS) desulfates a number of 3 beta-hydroxysteroid sulfates, converting inactive steroid hormone to the active form. We have established an enzyme-linked immunosorbent assay (ELISA) of STS by using polyclonal antibody against STS purified from human placenta to measure the amount of the enzyme protein in sera. ELISA was performed by a 'Sandwich' method using a peroxidase conjugated anti-STS IgG Fab' fragment. A range of STS of 10-1,500 ng/ml in serum was assayed by this method. When the serum STS from the patients with gynecologic carcinomas was assayed by the ELISA, the level was significantly elevated in endometrial carcinoma (P < 0.05) and ovarian carcinoma (P < 0.01), respectively, as compared with that of normal healthy women.
Sugawara
T
T
Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.
Honke
K
K
Gasa
S
S
Tanaka
T
T
Fujimoto
S
S
Makita
A
A
D000328:Adult; D000368:Aged; D000375:Aging / Q000097:blood; D000818:Animals; D001192:Arylsulfatases / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western; D002277:Carcinoma / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005833:Genital Neoplasms, Female / Q000201:enzymology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D008297:Male; D008875:Middle Aged; D011817:Rabbits; D012016:Reference Values; D043266:Steryl-Sulfatase
D007140:Immunoglobulin Fab Fragments; D001192:Arylsulfatases; D043266:Steryl-Sulfatase