Receptor-mediated Mn2+ influx in rat hepatocytes: comparison of cells loaded with Fura-2 ester and cells microinjected with Fura-2 salt.
In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not. The pattern of Fura-2 quenching by Mn2+ only correlated with the movement of Mn2+ across the plasma membrane.
Kass
G E
GE
Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
Webb
D L
DL
Chow
S C
SC
Llopis
J
J
Berggren
P O
PO
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D004952:Esters; D016257:Fura-2; D006873:Hydroquinones / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008345:Manganese / Q000378:metabolism*; D008845:Microinjections; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012492:Salts; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
D004952:Esters; D006873:Hydroquinones; D011956:Receptors, Cell Surface; D012492:Salts; D013729:Terpenes; C019359:2,5-di-tert-butylhydroquinone; D008345:Manganese; D019284:Thapsigargin; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium; D016257:Fura-2