Rapid and sensitive high-performance liquid chromatographic assay for novobiocin in human serum.
In this paper we present a new HPLC method for the determination of novobiocin in human serum. The assay uses mitomycin C as an internal standard, protein precipitation with acetonitrile, an ODS reversed-phase column with an isocratic mobile phase of acetonitrile-0.01 M phosphoric acid (80:20, v/v), and UV detection at 340 nm. The assay has a lower limit of quantitation of 1 microgram/ml and is linear over the range of 1-1000 micrograms/ml. The assay is ideally suited for use in clinical trials as it requires minimal amounts of serum, is highly sensitive and reproducible, is performed with minimal sample preparation, and involves a short run time. It should prove important in evaluating the potential of novobiocin as a means to modulate resistance to antineoplastic chemotherapy and in therapeutic drug monitoring of the growing number of patients receiving novobiocin to control methicillin-resistant Staphylococcus aureus infections.
Zuhowski
E G
EG
Division of Developmental Therapeutics, University of Maryland Cancer Center, Baltimore 21201.
Gutheil
J C
JC
Egorin
M J
MJ
D001798:Blood Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D008875:Middle Aged; D009675:Novobiocin / Q000097:blood*; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet
D001798:Blood Proteins; D009675:Novobiocin