title: In vitro fertilization of androgen sterilized mice.
abstract: In order to explore the effect of neonatal androgen administration on oocyte quality, ovulation induction and in vitro fertilization (IVF) were performed in androgen-sterilized mice. Androgen sterilized mice were produced by the subcutaneous injection of testosterone propionate (TP) at 5 days of age and ovulation induction was performed by pregnant mare's serum gonadotropin (PMSG)-human chorionic gonadotropin (hCG) treatment at 9 to 13 weeks old. The number of oocytes ovulated in TP-injected mice was 19.9 +/- 2.0 (Mean +/- SE) and was significantly less than that in normal mice (37.8 +/- 1.9; P < 0.01). The fertilization rate of oocytes retrieved from TP-treated mice (38.8%) was significantly lower than that from normal mice (60.3%; P < 0.01). These results indicate that neonatal androgen treatment has a detrimental effect on oocyte maturity.
authors.affiliation: Department of Obstetrics and Gynecology, Gunma University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kamijo
authors.forename: H
authors.initials: H
authors.lastname: Mizunuma
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Ibuki
mesh_terms: D000818:Animals; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009865:Oocytes / Q000502:physiology; D010062:Ovulation Induction; D011247:Pregnancy; D013245:Sterilization, Reproductive; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D013739:Testosterone