6-Fluoropyridoxol: a novel probe of cellular pH using 19F NMR spectroscopy.
6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (approximately 10 ppm acid/base shift) and a pKa approximately 8.2 appropriate for physiological investigations. Using 19F NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and 31P NMR spectroscopy.
Mehta
V D
VD
Department of Radiology, University of Texas Southwestern Medical Center, Dallas 75235-9058.
Kulkarni
P V
PV
Mason
R P
RP
Constantinescu
A
A
Aravind
S
S
Goomer
N
N
Antich
P P
PP
D000818:Animals; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011730:Pyridoxal / Q000031:analogs & derivatives* / Q000737:chemistry; D011817:Rabbits
D007202:Indicators and Reagents; D011730:Pyridoxal; C044385:6-fluoropyridoxal