Investigation of the poultry idiotypic network using Pasteurella multocida.
The idiotypic network in leghorn laying hens was investigated by inoculating hens with a 35.5 kD outer membrane protein of Pasteurella multocida (Pm35.5), idiotype (Id), or Pm35.5 anti-Id. Egg yolks were analyzed for the presence of Id, anti-Id, or anti-anti-Id. Anti-Pm35.5 antibodies (Id) were not titered to an end-point but were present in high concentrations. The presence of anti-Id antibody in yolk was demonstrated by the inhibition of Pm35.5 binding to Id by anti-Id using a flow microsphere inhibition immunoassay. Inhibition of Pm35.5 binding to Id caused by different anti-Id preparations ranges from 51.5 to 56.1%. Not all of the anti-Id bound to a paratope-associated Id, since 8.3-12.8% of the fluoresceinated anti-Id bound to Id-coated beads in the presence of excess Pm35.5. We confirmed that a portion of the anti-Id antibodies was an internal image of the Pm35.5 Id and could mimic antigen by demonstrating that anti-Id inoculated in naive hens caused the synthesis of anti-anti-Id antibodies that bound to Pm35.5 in enzyme linked immunosorbent assay. Monoclonal anti-Id antibodies were also capable of inhibiting Pm35.5 binding to Pm35.5 Id coated microspheres and inducing anti-anti-Id antibodies in BALB/cJ mice that reacted with the original antigen, Pm35.5. Our investigation has also shown that idiotypic antibodies to P. multocida were transferred from chicken to egg.
Zhang
H
H
College of Veterinary Medicine, Mississippi State University, Mississippi State 39762.
Ainsworth
A J
AJ
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D002846:Chromatography, Affinity / Q000662:veterinary; D004530:Egg Yolk / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007112:Immunity, Maternally-Acquired / Q000276:immunology; D007074:Immunoglobulin G / Q000302:isolation & purification; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D016979:Pasteurella multocida / Q000276:immunology*
D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes