Isolation of a mRNA encoding a nucleoside diphosphate kinase from tomato that is up-regulated by wounding.
A cDNA clone (TAB2) encoding a nucleoside diphosphate (NDP) kinase has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) cDNA library. The clone is 590 bp long and exhibits a high degree of sequence identity with spinach NDP kinases I and II, Pisum sativum NDP kinase I, Arabidopsis thaliana NDP kinase, Drosophila melanogaster NDP kinase, Dictyostelium discoideum NDP kinase and human Nm 23-H1 and Nm23-H2. Northern analysis has revealed that the mRNA encoded by TAB2 is up-regulated in both leaf and stem tissue in response to wounding. The increase is apparent within 1 h of wounding and is not further elevated by application of ethylene. Southern blot analysis indicates that TAB2 is a member of a small gene family.
Harris
N
N
Department of Physiology and Environmental Science, Faculty of Agricultural and Food Sciences, University of Nottingham, Loughborough, Leics, UK.
Taylor
J E
JE
Roberts
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology; D003001:Cloning, Molecular; D004023:Dictyostelium / Q000201:enzymology; D004330:Drosophila / Q000201:enzymology; D007887:Fabaceae / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D015723:Gene Library; D008969:Molecular Sequence Data; D009701:Nucleoside-Diphosphate Kinase / Q000096:biosynthesis* / Q000235:genetics; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D014675:Vegetables / Q000201:enzymology*
D012333:RNA, Messenger; D009701:Nucleoside-Diphosphate Kinase