Manipulations of germ-cell populations in the gonad of the fowl.
1. Embryos of the domestic fowl have been partially sterilised by injecting the drug busulphan into 24-h incubated eggs. 2. Some of these embryos were injected with primordial germ cells (PGCs) after 55 h of incubation to attempt to repopulate the gonads. 3. Primordial germ cells transfected with a defective retrovirus containing the reporter gene lac Z were shown to settle in these sterilised gonads. 4. Quantitative histology of 6-d embryos showed that busulphan produced 75% sterilisation but that PGCs could repopulate these gonads. 5. The technique of producing such germ line chimaeras is of value in studying cell kinetics, gonad differentiation and the production of transgenics.
Bresler
M
M
Department of Pure and Applied Zoology, University of Reading, England.
Behnam
J
J
Luke
G
G
Simkiss
K
K
D000818:Animals; D002066:Busulfan / Q000008:administration & dosage* / Q000633:toxicity; D002642:Chick Embryo / Q000166:cytology / Q000187:drug effects*; D005798:Genes, Bacterial; D005854:Germ Cells / Q000166:cytology / Q000187:drug effects; D013245:Sterilization, Reproductive / Q000379:methods; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis
D001616:beta-Galactosidase; D002066:Busulfan