title: Production and characterization of new monoclonal antibodies that distinguish subsets of mink lymphoid cells.
abstract: Several hybridoma clones that produce monoclonal antibodies (MAbs) reacting with subpopulations of mink lymphoid cells were established. Two of the MAbs, MTS-4.3 and MTS-9.3, reacted with relatively small populations of surface immunoglobulin (Ig)-negative (Ig-) lymphocytes. MTS-4.3+ and MTS-9.3+ cells were distributed in the thymic cortex and medulla, paracortical areas of lymph nodes, and periarterial lymphoid sheaths of the spleen, indicating that these MAbs identify T lymphocytes. Another MAb, MTB-5.6, reacted with a large proportion of surface Ig+ lymph node cells, but not with surface Ig- cells. In immunohistochemistry this MAb stained dendritic epithelial cells of thymic cortex, large polygonal cells of thymic medulla, a large proportion of lymphocytes in the mantle zone of lymphoid follicles, dendritic-shaped cells of paracortical area, and some lymphocytes and macrophage-like cells of medullary cords and sinuses of lymph nodes. The expression of the cell-surface antigen reacting with MTB-5.6 on Ig+ lymph node cells was increased after concanavalin A stimulation. These new reagents may be useful to analyze cellular basis of the abnormal immune responses observed in Aleutian mink disease, a classical model of human autoimmune diseases.
authors.affiliation: Laboratory of Persistent Viral Diseases, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840.
authors.forename: M
authors.initials: M
authors.lastname: Miyazawa
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: G J
authors.initials: GJ
authors.lastname: Spangrude
authors.forename: J B
authors.initials: JB
authors.lastname: Wolfinbarger
authors.forename: M E
authors.initials: ME
authors.lastname: Bloom
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006825:Hybridomas / Q000276:immunology; D007132:Immunoglobulin Isotypes; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008907:Mink / Q000276:immunology*; D011247:Pregnancy; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes