Low oxygen enhances endothelin-1 (ET-1) production and responsiveness to ET-1 in cultured cardiac myocytes.
To understand the pathophysiological role of ET-1 in heart, the ET-1 production ability and the responsiveness to ET-1 in cultured neonatal rat cardiac myocytes were compared between high (50%) and low (21%) oxygen atmosphere culture conditions. The amount of immunoreactive ET-1 secreted by the cardiac myocytes into the culture medium was much higher at the low oxygen condition. An analysis of ET-1 binding capacity to the cardiac myocytes revealed that the receptor number of ET-1 was about two-fold at low oxygen culture condition. In fact, an addition of ET-1 (1nM) increased in protein synthesis at the low but not the high oxygen culture condition. Thus, the increase in ET-1 production and responsiveness to ET-1 in the cardiac myocytes at the low oxygen culture condition suggests that autocrine ET-1 might be involved in the maintenance of beating ability in heart at hypoxia.
Kagamu
H
H
Cell Biology Laboratory, National Institute of Biosciences and Human Technology-Ibaraki, Japan.
Suzuki
T
T
Arakawa
M
M
Mitsui
Y
Y
D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D016232:Endothelins / Q000096:biosynthesis* / Q000502:physiology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017466:Receptors, Endothelin / Q000378:metabolism
D016232:Endothelins; D017466:Receptors, Endothelin