title: Cell culture adaptation of astrovirus involves a deletion.
abstract: Astroviruses have been adapted to culture by serial blind passage in primary human embryo cells. All viruses thus adapted possess a 45-nucleotide deletion relative to fecal viruses or isolates made in CaCo-2 cells; this deletion may be responsible for the change in host cell range.
authors.affiliation: School of Biological Sciences, University of Surrey, Guildford, United Kingdom.
authors.forename: M M
authors.initials: MM
authors.lastname: Willcocks
authors.forename: N
authors.initials: N
authors.lastname: Ashton
authors.forename: J B
authors.initials: JB
authors.lastname: Kurtz
authors.forename: W D
authors.initials: WD
authors.lastname: Cubitt
authors.forename: M J
authors.initials: MJ
authors.lastname: Carter
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D001257:Mamastrovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014779:Virus Replication
chemical_list: D017931:DNA Primers; D014764:Viral Proteins