[Detection of anti-gonococcal antibodies using a dot immunobinding assay in a high risk female population].
The bacteriological diagnosis of gonorrhea in women has a low efficiency. A dot immunobinding assay, using whole piliated Neisseria gonorrhoeae cells, was used to detect antigonococcal antibodies in 300 prostitutes in whom a parallel Thayer-Martin culture of endocervical secretion was performed. Twenty sera from men with bacteriologically confirmed gonorrhoea were used as positive controls and 20 sera from children without history of gonococcal infections, as negative controls. Antibodies were detected in 49.7% of women and culture was positive in only one. All men with gonorrhea has positive titers and all sera from children were negative, except one that had a cross-reaction. Test sensitivity in the female population was 1 and specificity 0.5. There were no false negative reactions. Although the test detected antibodies in high risk women, there were cross-reactions. Therefore, the technique should be improved, using a more specific antigen.
Soto
L
L
Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
Rodríguez
G
G
Iglesias
T
T
Loyola
A
A
Boehme
C
C
Polette
M
M
Díaz
M
M
D000907:Antibodies, Bacterial / Q000097:blood*; D002582:Cervix Mucus / Q000382:microbiology; D002648:Child; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D008297:Male; D009344:Neisseria gonorrhoeae / Q000276:immunology* / Q000302:isolation & purification; D012307:Risk Factors; D012680:Sensitivity and Specificity; D011477:Sex Work
D000907:Antibodies, Bacterial
Detección de anticuerpos antigonocócicos por dot immunobinding assay en una población femenina de alto riesgo.