Preimplantation embryo sexing by polymerase chain reaction amplification of the sry gene on single mouse blastomeres.
Accurate and rapid sex determination of preimplantation embryos has great potential both in animal breeding and in human pathology. In the past, sex determination has been accomplished by cytogenetic or immunologic means and by polymerase chain reaction amplification of Y-chromosome-specific repetitive sequences. More recently, amplification of the Y-specific single-copy ZFY gene has been used in humans for sex determination of preimplantation embryos. The experiments reported here indicate that another Y-chromosome-specific single-copy gene, the sex-determining region gene (sry) can be successfully amplified from single mouse blastomeres. Blastocysts positive for sry amplification were reimplanted to foster mothers, and six of six newborns were male. We conclude that sry gene amplification can represent a good marker for embryo sex determination.
Mulder
L C
LC
Joint Research Center, Center of European Communities, Ispra (Varese), Italy.
Sacco
M G
MG
Mangiarini
L
L
Brown
J
J
Collotta
A
A
Villa
A
A
De Giovanni
A M
AM
Vezzoni
P
P
Clerici
L
L
D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000145:classification*; D001757:Blastomeres; D004268:DNA-Binding Proteins / Q000235:genetics*; D004624:Embryo Transfer; D005260:Female; D005819:Genetic Markers; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis / Q000379:methods*; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014998:Y Chromosome
D004268:DNA-Binding Proteins; D005819:Genetic Markers; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; C497746:Sry protein, mouse; D014157:Transcription Factors