Essential involvement of interleukin-8 in neutrophil recruitment in rabbits with acute experimental arthritis induced by lipopolysaccharide and interleukin-1.
Rheumatoid arthritis and related inflammatory joint diseases are characterized by massive infiltration of polymorphonuclear cells (PMN) into inflamed joints. Interleukin-8 (IL-8) has recently been identified as a leukocyte chemotactic and activating factor produced by activated tissue cells as well as monocytes/macrophages. Examination was made of the involvement of IL-8 in acute arthritis induced by injecting lipopolysaccharide (LPS) or interleukin-1 alpha (IL-1 alpha) into the joints of rabbits. The neutralizing antibody to rabbit IL-8 blocked almost completely the infiltration of PMN into the joints and provided protection from damage to tissue in the early phase of inflammation induced by LPS or IL-1 alpha. Mononuclear cell infiltration observed later was not inhibited by this antibody. This is the first paper to clearly demonstrate that IL-8 is an essential and major mediator determining whether PMN infiltration will occur in the early phase of experimental acute arthritis.
Akahoshi
T
T
Department of Internal Medicine, Kitasato University School of Medicine, Japan.
Endo
H
H
Kondo
H
H
Kashiwazaki
S
S
Kasahara
T
T
Mukaida
N
N
Harada
A
A
Matsushima
K
K
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001168:Arthritis / Q000209:etiology / Q000276:immunology* / Q000473:pathology*; D004195:Disease Models, Animal; D007375:Interleukin-1 / Q000494:pharmacology; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D009500:Neutralization Tests; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology*; D011817:Rabbits
D000906:Antibodies; D007375:Interleukin-1; D016209:Interleukin-8; D008070:Lipopolysaccharides