title: Induction of differentiation by radiation and hyperthermia in neuroblastoma-glioma hybrid cells.
abstract: The effects of either radiation or hyperthermia on the differentiation potential of NG108-15, a neuroblastoma-glioma hybrid cell line, were studied. After radiation and hyperthermia, the outgrowth of neurites from NG108-15 cells was potentiated, and polarizing current and voltage pulses induced a distinct action potential and a diphasic (inward following outward) current, respectively. An increase in the specific activity of acetylcholinesterase was also observed. In addition, both treatments induced an elevation of the concentration of intracellular calcium in some cells. The increase in intracellular calcium concentration caused by applying the calcium ionophore, A23187, induced differentiation. It is suggested that both the radiation- and the hyperthermia-induced increases of electrical excitability and acetylcholinesterase activity may have originated from an increase in intracellular Ca2+ concentration.
authors.affiliation: Department of Radiology, Shimane Medical University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sugihara
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita
authors.forename: K
authors.initials: K
authors.lastname: Enomoto
authors.forename: T
authors.initials: T
authors.lastname: Maeno
authors.forename: T
authors.initials: T
authors.lastname: Ishida
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis; D002118:Calcium / Q000032:analysis; D002454:Cell Differentiation; D048429:Cell Size; D005910:Glioma; D006358:Hot Temperature; D006822:Hybrid Cells / Q000528:radiation effects; D008564:Membrane Potentials; D009447:Neuroblastoma; D009474:Neurons / Q000502:physiology* / Q000528:radiation effects*; D014407:Tumor Cells, Cultured
chemical_list: D000110:Acetylcholinesterase; D002118:Calcium