Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals.
The formation of the lipophorin-lipopolysaccharide (LPS) complex in Bombyx mori hemolymph and its role in LPS detoxification were explored. LPS, an antibacterial protein inducer in insects, was injected into B. mori larvae. Analytical density gradient ultracentrifugation revealed that after injection the LPS peak shifts to a zone of lower density with time. The shifted peak was identified as the lipophorin-LPS complex. This complex formation was also achieved in an in vitro mixture of cell-free hemolymph and LPS at 25 degrees C but not at 1 degree C. The lipophorin-LPS complex had a significantly lower capacity to elicit the mRNA of cecropin B, an antibacterial protein. The biological activity of reextracted LPS from the complex was slightly reduced in the Limulus test and no structural modification was observed in sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These results suggested that the formation of lipophorin-LPS strikingly reduces the cecropin inducibility of LPS without any structural change in LPS. Similar serum lipoprotein-LPS complex formation and reduction of biological activities of LPS were also observed in mammals. We, therefore, suggest that the formation of the serum lipoprotein-LPS complex is a common pathway to inactivate LPS both in insects and in mammals.
Kato
Y
Y
Laboratory of Biological Defense, National Institute of Sericultural and Entomological Science, Ibaraki, Japan.
Motoi
Y
Y
Taniai
K
K
Kadono-Okuda
K
K
Yamamoto
M
M
Higashino
Y
Y
Shimabukuro
M
M
Chowdhury
S
S
Xu
J
J
Sugiyama
M
M
D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry; D006458:Hemolymph / Q000737:chemistry / Q000378:metabolism*; D008658:Inactivation, Metabolic; D007301:Insect Hormones / Q000235:genetics; D019476:Insect Proteins; D007814:Larva; D008070:Lipopolysaccharides / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D008074:Lipoproteins; D008297:Male; D008657:Metabolic Clearance Rate; D011485:Protein Binding; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects
D002352:Carrier Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D008070:Lipopolysaccharides; D008074:Lipoproteins; D012333:RNA, Messenger; C032830:lipophorin; C031162:cecropin B protein, Insecta