A rapid method for whole mount preparations of mammalian oocytes and early embryos.
Whole mounts of mouse oocytes and embryos are useful for observing intracellular structures while preserving morphological integrity. This method is inconvenient for rapid processing of a large number of specimens because washing each specimen in a protein-free solution is required prior to transfer into the fixative. We have developed a new fixative which does not cause protein precipitation which can be added directly to the culture medium. Specimens can be preserved in culture dishes for at least one month, and processed for cytological observation at a convenient time. When stained with hematoxylin, details of cellular structures such as nuclei, nucleoli, chromosomes and spindle microtubules can be observed while maintaining the organization of the organelles.
Moses
R M
RM
Department of Zoology, University of Toronto, Ontario, Canada.
Masui
Y
Y
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000033:anatomy & histology* / Q000648:ultrastructure; D004626:Embryology / Q000379:methods; D005260:Female; D005404:Fixatives; D016591:Histocytological Preparation Techniques; D051379:Mice; D009865:Oocytes / Q000166:cytology* / Q000648:ultrastructure
D003470:Culture Media; D005404:Fixatives