title: [Measurement of the number of single-strand breaks in DNA of irradiated leukemia P-388 using alkaline denaturation].
abstract: Methods, used for indirect determination of single-thread breaks' quantity in DNA, based on it's denaturation in alkali medium and further determination of double-thread DNA quantity by it's fluorescence has been adapted to the measuring of thread disturbances on leukemia P-388 cells. Influence of cells' and alkali concentrations and the time of denaturation of quantity of double-thread DNA has been investigated.
authors.forename: V V
authors.initials: VV
authors.lastname: Fomin
authors.forename: M B
authors.initials: MB
authors.lastname: Borisov
authors.forename: A V
authors.initials: AV
authors.lastname: Zaĭtsev
authors.forename: D Iu
authors.initials: DIu
authors.lastname: Blokhin
mesh_terms: D000818:Animals; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D007941:Leukemia P388 / Q000235:genetics*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009691:Nucleic Acid Denaturation; D012972:Sodium Hydroxide; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D012972:Sodium Hydroxide
vernacular_title: Izmerenie kolichestva odnonitevykh razryvov v DNK obluchennykh kletok leĭkoza P-388 metodom shchelochnoĭ denaturatsii.