Transition from normal to premutated alleles in fragile X syndrome results from a multistep process.
In fragile X syndrome, the most common cause of inherited mental retardation, phenotypic expression has been linked to a region containing a repetitive sequence, (CGG)n, that appears to lengthen dramatically in fragile X patients and to show length variation in normal individuals. In order to investigate possible mechanisms responsible for further expansion of CGG in the normal population, we selected 31 normal unrelated X chromosomes carrying either the high-risk DX204-AC155 or DX196-AC151 haplotypes, as defined by the flanking microsatellites, DXS548 and FRAXAC2. Nearly 100% of CGGs with more than 35 repeats were found on DX204-AC155 haplotypes, with a mean length significantly higher and much more variable than in normal individuals carrying other haplotypes including the high-risk haplotype DX196-AC151. These findings suggest that the transition from the normal to the abnormal range occurs by a multistep process, a primary event, such as unequal crossing-over, leading to increased size and moderate instability of the repeat, and from which DNA polymerase slippage could lead to recurrent premutations. Our results also suggest that the upper limit of the normal range is roughly 35 repeats in the fragile X gene. The 36-54 repeats range would define an intermediate allele only observed, up to now, in DX204-AC155 fragile X chromosomes.
Montagnon
M
M
Centre d'Etudes de Biologie Prénatale, Paris, France.
Bogyo
A
A
Deluchat
C
C
Jokic
M
M
Chateau
C
C
Taillandier
A
A
Thomas
F
F
Simon-Bouy
B
B
Boué
J
J
Serre
J L
JL
D000704:Analysis of Variance; D003434:Crossing Over, Genetic; D004252:DNA Mutational Analysis; D005600:Fragile X Syndrome / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D012091:Repetitive Sequences, Nucleic Acid