Detection of epidermal growth factor receptor gene amplification in human squamous cell carcinomas using fluorescence in situ hybridization.
The gene for epidermal growth factor receptor (EGFR) is associated with development of certain human cancers. In this study, we employed the improved fluorescence in situ hybridization technique to detect EGFR gene amplification in cell lines and tissue sections from human squamous cell carcinomas. We detected multiple distinct signals as arrayed amplicons on metaphase chromosomes and interphase nuclei of tumor cells. Our results provide a basis for rapid and quantitative DNA diagnosis of the EGFR gene amplification in individual cells of tumor specimens.
Shimizu
N
N
Department of Molecular Biology, Keio University School of Medicine, Tokyo.
Wang
Y
Y
Minoshima
S
S
Ishitoya
J
J
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002874:Chromosome Mapping; D066246:ErbB Receptors / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D014407:Tumor Cells, Cultured
D066246:ErbB Receptors