title: Characterization and transcriptional regulation of rat glutamine tRNA-encoding genes: repression of tRNA(UUGGln) synthesis.
abstract: In rat liver, the amount of tRNA(UUGGln) (RG-2) was found to be approx. 20% of that of tRNA(CUGGln) (RG-1). The independent RG-1 and RG-2 genes were isolated from a rat genomic library together with four RG-related genes containing two to five alterations in their coding regions. Sequence analysis demonstrated that there was no difference between the internal promoter sequences of RG-1 and RG-2. However, interestingly, the transcriptional activity of RG-1 was approximately four-times higher than that of RG-2 in an in vitro transcription reaction. Replacement of the 5'-flanking sequence of RG-2 by the corresponding sequence of RG-1 or by a plasmid DNA sequence caused activation of RG-2 transcription. Gel retardation assay demonstrated that the 5'-flanking region of RG-2 contained a unique sequence specifically recognized by a nuclear protein. Taken together, these results strongly suggest that the transcriptional activity of RG-2 might be negatively regulated by the binding of a nuclear protein at a specific site in the 5'-flanking region of the gene.
authors.affiliation: Biophysics Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Nemoto
authors.forename: T
authors.initials: T
authors.lastname: Kohda
authors.forename: H
authors.initials: H
authors.lastname: Mizushima
authors.forename: H
authors.initials: H
authors.lastname: Yoshikura
authors.forename: H C
authors.initials: HC
authors.lastname: Schröder
authors.forename: W E
authors.initials: WE
authors.lastname: Müller
authors.forename: Y
authors.initials: Y
authors.lastname: Kuchino
mesh_terms: D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D005796:Genes / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012351:RNA, Transfer, Gln / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012351:RNA, Transfer, Gln