Cooperative action between band 3 and glycophorin A in human erythrocytes: immobilization of band 3 induced by antibodies to glycophorin A.
The ability of transmembrane receptor proteins to change their association with the cytoskeleton in response to ligand binding seems to be a key mechanism of signal transduction across membranes. To investigate the molecular features of this mechanism we have used the red cell membrane as a model system to study signal transduction through the integral protein, glycophorin A. In these studies the lateral mobility of integral proteins was measured in situ by fluorescence recovery after photobleaching, and membrane rigidity was characterized by micropipette aspiration technique. We found that binding either a monoclonal antibody or its monovalent Fab to the exoplasmic domain of glycophorin A in normal red cells immobilized the receptor and rigidified the membrane. Further, immobilization and rigidification did not occur when antibodies were bound to Miltenberger V cells containing a mutant form of glycophorin A lacking the cytoplasmic domain. These results imply that the site of the immobilization/rigidification lies within the membrane skeletal structure, not in exofacial receptor crosslinking, and requires the extended cytoplasmic domain of normal glycophorin A. In addition, we found that glycophorin A immobilization and membrane skeletal rigidification were accompanied by immobilization of band 3 receptors. This unexpected result indicates a cooperative coupling between liganded glycophorin A, band 3, and the membrane skeleton. We speculate that cooperation of this type may represent a general mechanism for cytoskeletal linkage and transformation initiated by receptors with short cytoplasmic sequences, such as integrins.
Knowles
D W
DW
Department of Physics, University of British Columbia, Vancouver, Canada.
Chasis
J A
JA
Evans
E A
EA
Mohandas
N
N
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000276:immunology / Q000378:metabolism*; D000906:Antibodies; D055592:Biophysical Phenomena; D001703:Biophysics; D002161:Camelids, New World / Q000097:blood; D003599:Cytoskeleton / Q000378:metabolism; D004907:Erythrocyte Deformability; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006021:Glycophorins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009154:Mutation; D011956:Receptors, Cell Surface / Q000378:metabolism; D013045:Species Specificity
D001457:Anion Exchange Protein 1, Erythrocyte; D000906:Antibodies; D006021:Glycophorins; D011956:Receptors, Cell Surface