T cell receptor V alpha gene usage in human T cells stimulated with SEE and SED.
V alpha gene usage in human T cells stimulated with SEE and SED was investigated by using polymerase chain reaction with specific primers. V beta 8 T cells from normal blood donors PBMC were sorted at day 5 after stimulation with SEE and analysed for TCR-V alpha gene usage. Whole T cells stimulated with anti-CD3 MoAb or SED were also analysed and compared at different time points after stimulation. There was no biased V alpha gene usage found as a response to either of the two superantigens. These results show that V alpha gene usage of human T cells stimulated with SEE or SED is normal.
Liu
J L
JL
Immunology Laboratory, Karolinska Institute, Stockholm, Sweden.
Hodara
V L
VL
Wigzell
H
H
D001483:Base Sequence; D015139:Blotting, Southern; D004768:Enterotoxins / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D018089:Superantigens / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D004768:Enterotoxins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; C049463:enterotoxin E, Staphylococcal; C019368:enterotoxin D, Staphylococcal