Capillary zone electrophoretic determination of histamine in fish.
Histamine, the principal causative agent in scombroid food poisoning, was analyzed in seafood by a new, rapid, and sensitive method using capillary zone electrophoresis (CZE) with UV detection at 210 nm. Incurred histamine in methanolic fish extracts migrated within 4 min in a fused silica capillary filled with 0.02 M citrate buffer, pH 2.5, under an applied potential of 375 V/cm. The analytical response was linear from 0.5 to 100 ppm histamine (correlation coefficient, r = 0.999). The coefficients of variation for migration time and peak area response were < 1 and < 3%, respectively. Recovery of histamine in fortified fish composites was satisfactory. CZE was considered for alternative application in seafood speciation.
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U.S. Food and Drug Administration, Northeast Regional Laboratory, Brooklyn, NY 11232-1593.
Sciacchitano
C J
CJ
D000818:Animals; D004586:Electrophoresis / Q000379:methods*; D006632:Histamine / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D012680:Sensitivity and Specificity; D014413:Tuna
D006632:Histamine