title: Equilibrium dialysis of metal-serum albumin. I. Successive stability constants of Zn(II)-serum albumin and the Zn(2+)-induced cross-linking self-association.
abstract: The binding of Zn(II) to human serum albumin (HSA) and bovine serum albumin (BSA) was intensively studied by equilibrium dialysis. The successive stability constants were obtained by least-squares fitting. For both the Zn(II)-HSA and Zn(II)-BSA systems, the successive stability constants are basically similar, though such constants for the latter generally slightly larger than those for the former; the order of magnitude of K1 and K2 was found to be approximately 10(5) M-1. The twelve binding sites found for Zn(II)-HSA and fourteen binding sites for Zn(II)-BSA can be divided into three and two different sets, respectively, but in both systems, there exist approximately three identical strongest binding sites. The binding equilibrium of Zn(II)-HSA markedly depends on the concentration of HSA. The type of Scatchard plots indicates the existence of the Zn(2+)-induced cross-linking self-association of HSA, which was found from Wyman plots to be the strongest at a concentration of Zn2+ in the range (4-6) x 10(-6) M. The maximum of the apparent self-association constants k2 so obtained was approximately 2.6 x 10(4) M-1. Such a self-association mechanism and the order of magnitude of K1 and K2 both to some extent support the inference that the zinc group ions are bound to the cysteinyl sulphur atoms.
authors.affiliation: Department of Chemistry, Nankai University, Tianjin, People's, Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Hu
authors.forename: J
authors.initials: J
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Hao
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: P
authors.initials: P
authors.lastname: Shen
mesh_terms: D000818:Animals; D002417:Cattle; D006801:Humans; D007700:Kinetics; D008433:Mathematics; D008962:Models, Theoretical; D012709:Serum Albumin / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
chemical_list: D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D015032:Zinc