title: Sympathetic nervous system involvement in essential hypertension: increased platelet noradrenaline coincides with decreased beta-adrenoreceptor responsiveness.
abstract: Platelet catecholamine content may reflect integrated plasma catecholamine concentrations over time. The present study aimed at examining sympathetic nervous system (SNS) involvement in essential hypertension by assessing platelet noradrenaline (NA) and typically beta-adrenoreceptor mediated responses to adrenaline (A) infusion as indices of sympathetic tone. Healthy white men were recruited by public advertising and screening (mean +/- SD): Hypertensives (n = 13, sitting blood pressure [BP] 153 +/- 13/106 +/- 7 mmHg, age 34 +/- 5 years, weight 83 +/- 10 kg) were compared to normotensives (n = 13, sitting BP 114 +/- 9/75 +/- 9 mmHg, age 30 +/- 6 years [n.s.], weight 82 +/- 9 kg [n.s.]). Loss of platelet granular contents (including NA) prior to analysis was minimized by studying young subjects (age range 20-40 years, minimal atherosclerosis), using arterial blood sampling, and processing blood immediately. These procedures resulted in plasma beta-thromboglobulin and platelet factor 4 levels which were not significantly different between groups. Sympathetic activation resulting from stress was minimized by not labelling subjects as either hypertensive or normotensive. Mean arterial platelet NA content was significantly higher in hypertensives (64 +/- 31 pg/mg of platelet weight) compared to normotensives (43 +/- 20 pg/mg, p < 0.05) both at baseline and following 35% expansion of the circulating platelet pool by A infusion (p < 0.05) and correlated with arterial NA in the hypertensives (r = 0.79, p < 0.002) but not in the normotensives (r = 0.04, n.s.). Similar increases in platelet and plasma A during infusion in both groups suggest unchanged platelet uptake capacity and plasma clearance in the hypertensive group.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Internal Medicine, University of Michigan, Ann Arbor.
authors.forename: S E
authors.initials: SE
authors.lastname: Kjeldsen
authors.forename: A J
authors.initials: AJ
authors.lastname: Zweifler
authors.forename: J
authors.initials: J
authors.lastname: Petrin
authors.forename: A B
authors.initials: AB
authors.lastname: Weder
authors.forename: S
authors.initials: S
authors.lastname: Julius
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D001794:Blood Pressure; D001798:Blood Proteins / Q000378:metabolism; D002395:Catecholamines / Q000097:blood; D004837:Epinephrine / Q000494:pharmacology; D006339:Heart Rate; D006439:Hemodynamics / Q000187:drug effects; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000503:physiopathology*; D008297:Male; D009638:Norepinephrine / Q000097:blood*; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D013564:Sympathetic Nervous System / Q000503:physiopathology*
chemical_list: D001798:Blood Proteins; D002395:Catecholamines; D011943:Receptors, Adrenergic, beta; D009638:Norepinephrine; D004837:Epinephrine