EPR evidence for nitric oxide formation via L-arginine-dependent way in stomach of mice in vivo.
Suspension of baker's yeast loaded with a specific trap of nitric oxide (NO), a complex of Fe2+ with exogenous diethyldithiocarbamate (DETC), was used for the detection of NO formed in mouse stomach at its adaptive relaxation in vivo. NO formation was determined by the increase of intensity of the ERP signal due to trapping of NO in mononitrosyl iron complex with DETC (MNIC-DETC) which appeared in yeast cells infused into the stomach. An increase in signal intensity was observed in stomach preparations isolated from mice when the doses of yeast suspensions injected p/o into mouse stomach for 40 min were increased. The intensity of this signal which was proportional to the concentration of MNIC-DETC in yeast cells was diminished when the NO-synthase inhibitor, NG-nitro-L-arginine was injected i/p into mice.
Mikoyan
V D
VD
Institute of Chemical Physics, Russian Academy of Sciences, Moscow.
Kubrina
L N
LN
Vanin
A F
AF
D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D004050:Ditiocarb / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005296:Ferrous Compounds / Q000737:chemistry; D005753:Gastric Mucosa / Q000378:metabolism*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D019335:Nitroarginine; D012441:Saccharomyces cerevisiae; D013270:Stomach / Q000187:drug effects
D005296:Ferrous Compounds; D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D004050:Ditiocarb