Identification of S-locus linked PCR fragments in rye (Secale cereale L.) by denaturing gradient gel electrophoresis.
Rye inbred lines segregating at the S-locus and homozygous at the Z-locus were investigated by PCR with primers derived from Brassica SLG-sequences. After denaturing gradient gel electrophoresis (DGGE), a 280 bp PCR-fragment displays a polymorphism perfectly correlated to the underlying S-genotypes. This is the first report on S-related DNA polymorphism in a bifactorial self-incompatibility system of the Poaceae.
Wehling
P
P
Institute of Applied Genetics, Hannover, Germany.
Hackauf
B
B
Wricke
G
G
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005821:Genetic Techniques; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012434:Secale / Q000235:genetics*