title: Methods and reagents. Slants and spots.
abstract: Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methods-reagents, available on the Internet. This month's column discusses some of the unexpected and sometimes unexplained blotches that may appear on your gels or film plates. For details on how to partake in the newsgroup, see the accompanying box.
authors.affiliation: National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201.
authors.forename: P N
authors.initials: PN
authors.lastname: Hengen
mesh_terms: D001345:Autoradiography; D015152:Blotting, Northern; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D007202:Indicators and Reagents; D009994:Osmolar Concentration
chemical_list: D007202:Indicators and Reagents; D004247:DNA; D004996:Ethidium