title: Different mass spectrometric approaches in the identification of endogenous methylated purine bases in urine extracts.
abstract: A preliminary identification of endogenous and exogenous methylated purine bases in urinary extracts of healthy and tumor-bearing subjects has been performed using high performance liquid chromatography, and tandem mass spectrometry (MS/MS). MS/MS gave particularly fast and sensitive analyses, allowing the simultaneous and rapid determination of 26 different urinary methylated purines. Both sets of data confirm that tumor-bearing patients show changed levels of methylated purine bases.
authors.affiliation: Istituto di Biochimica ed Enzimologia, Siena, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Porcelli
authors.forename: R
authors.initials: R
authors.lastname: Pagani
authors.forename: L
authors.initials: L
authors.lastname: Lorenzini
authors.forename: A
authors.initials: A
authors.lastname: De Martino
authors.forename: S
authors.initials: S
authors.lastname: Catinella
authors.forename: P
authors.initials: P
authors.lastname: Traldi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015179:Colorectal Neoplasms / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry; D008745:Methylation; D008970:Molecular Weight; D011687:Purines / Q000652:urine*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011687:Purines