Intracellular distribution of cystine in cystine-loaded proximal tubules.
Cellular cystine loading with cystine dimethyl ester has been shown to inhibit transport in proximal convoluted tubules perfused in vitro and decrease the rate of oxygen consumption in suspensions of proximal tubules. The present study was designed to examine the intracellular distribution of cystine in this model of the Fanconi syndrome of cystinosis and to determine whether cystine or its degradation product, cysteine, is the cytotoxic agent in cystine-loaded rabbit proximal tubules. Tubules were incubated with 2 mmol/L cystine dimethyl ester for 10 min at 37 degrees C and subjected to cellular fractionation. The intralysosomal cystine content (272 +/- 125 nmol/mg protein) was significantly higher than that measured in the nucleus (8.7 +/- 2.0 nmol/mg protein) and cytosol (9.8 +/- 4.0 nmol/mg protein (p < 0.05). Electron micrographs of tubules loaded with cystine depicted large swollen lysosomes. To determine whether cystine or its breakdown product, cysteine, was the cytotoxic agent in tubules incubated with cystine dimethyl ester, the intracellular cystine and cysteine contents were measured and found to be 86.5 +/- 14.8 and 5.7 +/- 1.7 nmol/mg protein, respectively. These tubules had a 50% decrease in the rate of O2 consumption. To examine whether the increased level of intracellular cysteine played a role in this decrease in O2 consumption, we loaded tubules with 2 mmol/L cysteine methyl ester for 10 min. Despite an intracellular cysteine concentration of 59.6 +/- 5.8 nmol/mg protein, cysteine-loaded tubules had a rate of O2 consumption equal to that measured in control tubules. Thus, intracellular cystine loading significantly increases intralysosomal cystine content.(ABSTRACT TRUNCATED AT 250 WORDS)
Sakarcan
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A
Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas 75235-9063.
Timmons
C
C
Baum
M
M
D000818:Animals; D002451:Cell Compartmentation; D003545:Cysteine / Q000378:metabolism / Q000633:toxicity; D003553:Cystine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000493:pharmacokinetics / Q000633:toxicity; D005198:Fanconi Syndrome; D007687:Kidney Tubules, Proximal / Q000737:chemistry* / Q000378:metabolism; D008247:Lysosomes / Q000737:chemistry*; D010101:Oxygen Consumption; D011817:Rabbits; D013347:Subcellular Fractions / Q000737:chemistry; D014018:Tissue Distribution
D003553:Cystine; D003545:Cysteine; C034582:cystine dimethyl ester