[The effect of structure of benzimidazoles on the character of forming intramolecular cross-links in DNA and chromatin].
An investigation of a number of benzimidazole class preparations, being distinguished by a position of aminomethyl substitutes, has been carried out. It has been shown, that the non-substituted preparation BIO-10 does not form UV-cross-links in DNA and chromatine; BIO-40, having one substitute in the position 2, causes the formation of inter-molecular cross-links DNA-DNA. The preparation BIO-50, having 2 aminomethyl groups in the imidazole nucleus positions 2 and 6, forms cross-links DNA-DNA and DNA-protein in chromatine. The generation of radicals by the preparations BIO-10 and BIO-50 has been studied by the EPR-method by use of spin trap. It has been demonstrated, that BIO-10, unlike BIO-50, actively generates superoxide. A supposition has been made, that an UV-formation of superoxide-radical in the presence of BIO-10 might be a reason of DNA-macromolecule destruction.
Mil'
E M
EM
Zhil'tsova
V M
VM
Biniukov
V I
VI
Zhizhina
G P
GP
Stoliarova
L G
LG
Kuznetsov
Iu P
IuP
D000818:Animals; D001562:Benzimidazoles / Q000737:chemistry*; D002417:Cattle; D002843:Chromatin / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D014466:Ultraviolet Rays
D001562:Benzimidazoles; D002843:Chromatin; D003432:Cross-Linking Reagents; D005609:Free Radicals; D004247:DNA
Vliianie struktury preparatov klassa benzimidazola na kharakter obrazovaniia vnutrimolekuliarnykh sshivok v DNK i khromatine.