title: Assisted fertilization by subzonal insemination and intracytoplasmic sperm injection.
abstract: The results of 600 consecutive treatment cycles of subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) are described in couples with failed fertilization after standard IVF or insufficient spermatozoa in the ejaculate for IVF. More oocytes were damaged by ICSI (16.3%) than by SUZI (8.5%) and the normal fertilization rate was substantially higher after ICSI (49.1% v. 16.6%). Subsequent development of two-pronuclear oocytes in vitro was 80% after SUZI and 73.9% after ICSI. Significantly more triple embryo replacements were carried out after ICSI than after SUZI. Embryo transfers were possible in 421 of the 600 cycles. There were 63 pregnancies after ICSI (215 transfers) and 23 after SUZI (156 transfers); 10 additional pregnancies were achieved after 50 transfers of a mixture of SUZI and ICSI embryos. The results of fetal karyotypes and follow-up of the children do not indicate an increase in congenital malformations.
authors.affiliation: Centre for Reproductive Medicine, University Hospital and Medical School, Vrije Universiteit Brussel, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Van Steirteghem
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Joris
authors.forename: Z
authors.initials: Z
authors.lastname: Nagy
authors.forename: C
authors.initials: C
authors.lastname: Staessen
authors.forename: M
authors.initials: M
authors.lastname: Camus
authors.forename: A
authors.initials: A
authors.lastname: Wisanto
authors.forename: E
authors.initials: E
authors.lastname: Van Assche
authors.forename: P
authors.initials: P
authors.lastname: Devroey
mesh_terms: D000328:Adult; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods*; D005500:Follow-Up Studies; D006801:Humans; D007248:Infertility, Male; D008297:Male; D008845:Microinjections; D009865:Oocytes; D011247:Pregnancy; D011256:Pregnancy Outcome; D012189:Retrospective Studies; D013048:Specimen Handling / Q000379:methods; D013094:Spermatozoa; D015044:Zona Pellucida