title: Shuttle mutagenesis of Legionella pneumophila: identification of a gene associated with host cell cytopathicity.
abstract: We performed shuttle mutagenesis of Legionella pneumophila. Mutants were screened for reduced cellular infectivity. Approximately 10% of the mutants had decreased cytopathicity. The DNA sequence of one locus was determined; the inferred amino acid sequence revealed homology with transport proteins including Escherichia coli TolC, Bordetella pertussis CyaE, and Alcaligenes eutrophus CzcC and CnrC.
authors.affiliation: Department of Microbiology, University of Michigan Medical School, Ann Arbor 48109.
authors.forename: J
authors.initials: J
authors.lastname: Arroyo
authors.forename: M C
authors.initials: MC
authors.lastname: Hurley
authors.forename: M
authors.initials: M
authors.lastname: Wolf
authors.forename: M S
authors.initials: MS
authors.lastname: McClain
authors.forename: B I
authors.initials: BI
authors.lastname: Eisenstein
authors.forename: N C
authors.initials: NC
authors.lastname: Engleberg
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D006801:Humans; D016952:Legionella pneumophila / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D016296:Mutagenesis
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; C088933:Hel protein, Legionella pneumophila