Iron deprivation-induced apoptosis in HL-60 cells.
Iron deprivation of HL-60 cells with deferoxamine B mesylate (DFO) induced apoptosis. DNA fragmentation became apparent with 10(-6) M DFO after 48 h treatment. The apoptosis peak according to the DNA histogram on flow cytometry and typical nuclear collapse and were observed microscopically after 48 h treatment with 10(-4) M DFO. Cells treated with 10(-4) M DFO for as little as 24 h were shown to be committed to apoptosis, as chromatin condensation progressed gradually thereafter.
Fukuchi
K
K
Department of Clinical Pathology, School of Medicine, Showa University, Tokyo, Japan.
Tomoyasu
S
S
Tsuruoka
N
N
Gomi
K
K
D017209:Apoptosis / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000187:drug effects; D003676:Deferoxamine / Q000494:pharmacology*; D006801:Humans; D000090463:Iron Deficiencies; D007951:Leukemia, Myeloid / Q000473:pathology; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D003676:Deferoxamine