title: 6-Fluoropyridoxol: a novel probe of cellular pH using 19F NMR spectroscopy.
abstract: 6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (approximately 10 ppm acid/base shift) and a pKa approximately 8.2 appropriate for physiological investigations. Using 19F NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and 31P NMR spectroscopy.
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical Center, Dallas 75235-9058.
authors.forename: V D
authors.initials: VD
authors.lastname: Mehta
authors.forename: P V
authors.initials: PV
authors.lastname: Kulkarni
authors.forename: R P
authors.initials: RP
authors.lastname: Mason
authors.forename: A
authors.initials: A
authors.lastname: Constantinescu
authors.forename: S
authors.initials: S
authors.lastname: Aravind
authors.forename: N
authors.initials: N
authors.lastname: Goomer
authors.forename: P P
authors.initials: PP
authors.lastname: Antich
mesh_terms: D000818:Animals; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011730:Pyridoxal / Q000031:analogs & derivatives* / Q000737:chemistry; D011817:Rabbits
chemical_list: D007202:Indicators and Reagents; D011730:Pyridoxal; C044385:6-fluoropyridoxal