Monoclonal B cells and restricted oligoclonal T cells in T-cell-rich B-cell lymphoma.
Immunophenotyping of lymphoma using paraffin-embedded lymphoid tissue is useful in identifying the large neoplastic B cells in T-cell-rich B-cell lymphoma (TRBL), but does not succeed in deciding clonality. We studied six cases to determine the clonal population of B and T cells of TRBL. Immunohistochemistry on frozen and paraffin-embedded material showed that the cellular population in all six cases consisted mainly of T cells; fewer than ten percent of the cells stained as B cells. However, in all cases, monoclonality of the immunoglobulin was helpful for diagnosing the B-cell neoplasia. Southern blot-yielded genetic analysis showed monoclonality of B cells in three cases, but no evidence of clonality in the T cells. Moreover, gene monoclonality has been detected in all cases examined by polymerase chain reaction, using the primers for the V and J regions of the immunoglobulin heavy chain gene. For T cells, the D and J regions of the T-cell receptor (TCR) beta chain showed the same patterns of oligoclonal bands in all cells, and the V and J regions of the TCR gamma chain showed the same bands in all. The expression of TCR V beta families was polyclonal but restricted.
Ohshima
K
K
First Department of Pathology, School of Medicine, Fukuoka University, Japan.
Masuda
Y
Y
Kikuchi
M
M
Sumiyoshi
Y
Y
Kobari
S
S
Yoneda
S
S
Takeshita
M
M
Kimura
N
N
D000368:Aged; D001402:B-Lymphocytes / Q000473:pathology*; D001483:Base Sequence; D015139:Blotting, Southern; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000473:pathology*; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D015335:Molecular Probes