[The effect of conditions of synthesis of immunolaccase conjugates on characteristics and composition of the compounds obtained].
Optimal conditions for preparing laccase conjugates by the periodate method have been selected. The effect of the initial molar ratio of IgG to laccase and pH of the medium on the composition of laccase conjugates was studied by the HPLC method. The maximum yield of the conjugates was observed, when laccase was oxidized with 0.12 M sodium periodate the pH of the medium was 8.5, and the initial molar ratio of IgG to laccase was 2:1. The conjugates can be stored for one year without any loss in immunological activity.
Skorobogat'ko
O V
OV
Dzhafarova
A N
AN
Iaropolov
A I
AI
D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000737:chemistry*; D042845:Laccase; D010088:Oxidoreductases / Q000737:chemistry* / Q000276:immunology; D010504:Periodic Acid / Q000737:chemistry
D007074:Immunoglobulin G; D010504:Periodic Acid; D010088:Oxidoreductases; D042845:Laccase
Vliianie usloviĭ sinteza immunolakkaznykh kon''iugatov na kharakteristiki i sostav poluchaemykh soedineniĭ.