title: Endotoxin pretreatment inhibits neutrophil proliferation and function.
abstract: Gut ischemia/reperfusion (I/R) induces lung injury by a mechanism that involves neutrophils (PMNs). We have previously shown that endotoxin (LPS), when administered after gut I/R, amplifies this lung injury, while treatment with LPS prior to gut I/R prevents lung injury. The purpose of this study was to determine whether LPS pretreatment (Pre Rx) alters the PMN inflammatory component of the gut I/R injury. Specifically, we focused on whether LPS Pre Rx effected (i) PMN stem cell proliferation, (ii) gut I/R-induced PMN priming, and (iii) gut I/R-induced PMN lung sequestration. Bone marrow was harvested from normal and LPS-pretreated (0.5 mg/kg, ip, 3 days prior) rats, and colony forming units--granulocyte/macrophage (CFU-GM) were quantitated using a soft agar culture technique. In another experiment, normal and LPS-pretreated rats were subjected to gut I/R (45 min superior mesenteric artery occlusion/6 hr reperfusion), and blood and lungs were then harvested. The in vivo priming of PMN was assessed by measuring the difference in superoxide production (O2-) with and without the activating stimulus, N-formylmethionyl-leveyl-phenylalanine (fMLP). The quantity of myeloperoxidase (MPO) was used as an index of the number of PMN sequestered in lung tissue.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, Denver General Hospital, Colorado 80204.
authors.forename: K
authors.initials: K
authors.lastname: Koike
authors.forename: F A
authors.initials: FA
authors.lastname: Moore
authors.forename: E E
authors.initials: EE
authors.lastname: Moore
authors.forename: C E
authors.initials: CE
authors.lastname: Trew
authors.forename: A
authors.initials: A
authors.lastname: Banerjee
authors.forename: V M
authors.initials: VM
authors.lastname: Peterson
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D002199:Capillary Permeability; D002455:Cell Division / Q000187:drug effects; D004731:Endotoxins / Q000494:pharmacology*; D007422:Intestines / Q000098:blood supply*; D008168:Lung / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology*; D012709:Serum Albumin / Q000378:metabolism; D013234:Stem Cells / Q000473:pathology
chemical_list: D004731:Endotoxins; D012709:Serum Albumin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009195:Peroxidase