title: Purification and partial characterization of isoforms of luteinizing hormone from the chicken pituitary gland.
abstract: Isoforms of chicken luteinizing hormone (cLH) were isolated from chicken pituitaries by differential extraction, sequential chromatography on HPLC cation- and anion-exchange columns, and gel-filtration chromatography. Three purified isoforms of the cLH had high biological potency in the chicken granulosa cell LH bioassay (4.21-7.4 x NIH-LH-S1 U/mg). One cLH isoform without detectable biological activity showed substantial immunological activity in a homologous cLH radioimmunoassay. The cLH isoforms contained negligible follicle-stimulating hormone activity, but some contained thyroid-stimulating hormone activity. The apparent molecular weight of cLH was 37,000 Da, and the holoprotein consisted of subunits with a molecular weight of approximately 17,000 Da.
authors.affiliation: USDA-ARS, Germplasm and Gamete Physiology Laboratory, Beltsville Agricultural Research Center, MD 20705.
authors.forename: K A
authors.initials: KA
authors.lastname: Krishnan
authors.forename: J A
authors.initials: JA
authors.lastname: Proudman
authors.forename: J M
authors.initials: JM
authors.lastname: Bahr
mesh_terms: D000818:Animals; D001681:Biological Assay; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D013006:Growth Hormone / Q000032:analysis; D007526:Isoelectric Point; D007986:Luteinizing Hormone / Q000032:analysis / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D010902:Pituitary Gland / Q000737:chemistry*; D011374:Progesterone / Q000378:metabolism; D011388:Prolactin / Q000032:analysis; D011863:Radioimmunoassay; D013237:Stereoisomerism; D013972:Thyrotropin / Q000032:analysis
chemical_list: D011374:Progesterone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D013006:Growth Hormone