title: Endothelin-1 inhibits insulin-stimulated glucose uptake in isolated rat adipocytes.
abstract: A single class of high affinity endothelin-1 (ET-1) binding sites with an apparent Kd of 350 pM and a binding capacity of 69,000 sites/cell was found in isolated rat adipocytes. Whereas ET-1 had no effect on basal glucose uptake, it inhibited insulin-stimulated glucose uptake in both a dose- and time-dependent manner. Insulin binding, however, was not altered. Thus, the present study demonstrates the presence of ET-1 receptor in isolated rat adipocytes and the inhibition of insulin-stimulated glucose uptake by ET-1 via a post-receptor mechanism.
authors.affiliation: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.
authors.forename: Y C
authors.initials: YC
authors.lastname: Chou
authors.forename: J C
authors.initials: JC
authors.lastname: Perng
authors.forename: C C
authors.initials: CC
authors.lastname: Juan
authors.forename: S Y
authors.initials: SY
authors.lastname: Jang
authors.forename: C F
authors.initials: CF
authors.lastname: Kwok
authors.forename: W L
authors.initials: WL
authors.lastname: Chen
authors.forename: J C
authors.initials: JC
authors.lastname: Fong
authors.forename: L T
authors.initials: LT
authors.lastname: Ho
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D016232:Endothelins / Q000378:metabolism / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016232:Endothelins; D007457:Iodine Radioisotopes; D005947:Glucose