Characterisation of immunoglobulin-binding leucocytes in carp (Cyprinus carpio L.).
This study demonstrates the immunoglobulin(Ig)-binding capacity of Ig-positive carp macrophages employing immunofluorescence and immunogold methods. These methods allow for the characterisation of the Ig-binding cells. After internalisation of fluorescent- or gold-labelled Ig (30 min at room temperature), most macrophages from the hindgut were able to bind added purified carp Ig, which could be demonstrated clearly with a second fluorescent or gold label. In pronephros, an important haemopoietic organ in fish, a limited number of monocyte-like cells also showed Ig binding. Pronephros macrophages and neutrophilic granulocytes appeared to be Ig-negative. The use of goat anti-mouse Ig gold particles bound by carp anti-goat antibodies revealed that, in addition to hindgut macrophages and pronephric monocyte-like cells, some lymphoid cells in both hindgut and pronephros cell suspensions were also able to bind Ig. The classic erythrocyte-antibody rosette assay resulted in a limited number of small rosettes in cell suspensions from both organs.
Koumans-van Diepen
J C
JC
Department of Experimental Animal Morphology and Cell Biology, Agricultural University, Wageningen, The Netherlands.
van de Lisdonk
M H
MH
Taverne-Thiele
A J
AJ
Verburg-van Kemenade
B M
BM
Rombout
J H
JH
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002347:Carps / Q000033:anatomy & histology / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006413:Hematopoietic System / Q000166:cytology / Q000276:immunology; D007136:Immunoglobulins / Q000378:metabolism*; D007150:Immunohistochemistry; D007422:Intestines / Q000166:cytology / Q000276:immunology; D007962:Leukocytes / Q000276:immunology* / Q000648:ultrastructure; D008264:Macrophages / Q000276:immunology; D016253:Microscopy, Immunoelectron; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis; D012397:Rosette Formation
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007136:Immunoglobulins