Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1.
Cell cycle arrest of Saccharomyces cerevisiae in G1 by the antimitogen alpha-factor is mediated by activation of a signal transduction pathway that results in inhibition of the cyclin-dependent kinase Cdc28-Cln. The Far1 protein is required for cell cycle arrest and associates with the Cdc28-Cln complex. The kinase activity of Cdc28-Cln was directly inhibited by Far1 both in vivo and in vitro, thus demonstrating that Far1 acts at the final step in the alpha-factor response pathway by inhibiting a G1 cyclin-dependent kinase.
Peter
M
M
Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448.
Herskowitz
I
I
D017489:CDC28 Protein Kinase, S cerevisiae / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism / Q000494:pharmacology*; D016193:G1 Phase; D000072235:Mating Factor; D010455:Peptides / Q000494:pharmacology; D010766:Phosphorylation; D006656:Protamine Kinase / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism / Q000494:pharmacology; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins
C082721:CLN2 protein, S cerevisiae; D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D016213:Cyclins; C081353:FAR1 protein, S cerevisiae; D005656:Fungal Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D000072235:Mating Factor; D006656:Protamine Kinase; D017489:CDC28 Protein Kinase, S cerevisiae