Characterization of a pollen-expressed gene encoding a putative pectin esterase of Petunia inflata.
From a pollen tube cDNA library of Petunia inflata, we isolated cDNA clones encoding a protein, PPE1, which exhibits sequence similarity with plant, bacterial, and fungal pectin esterases. Genomic clones containing the PPE1 gene were isolated using cDNA for PPE1 as a probe, and comparison of the cDNA and genomic sequences revealed the presence of a single intron in the PPE1 gene. During pollen development, PPE1 mRNA was first detected in anthers containing uninucleate microspores; it reached the highest level in mature pollen and persisted at a high level in in vitro germinated pollen tubes. The observed expression pattern of the PPE1 gene suggests that its product may play a role in pollen germination and/or tube growth.
Mu
J H
JH
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802.
Stains
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JP
Kao
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D010368:Pectins / Q000378:metabolism*; D063245:Plant Development; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011058:Pollen / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase