A new ATP-independent DNA endonuclease from Schizosaccharomyces pombe that recognizes cyclobutane pyrimidine dimers and 6-4 photoproducts.
We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA endonuclease (SPDE) catalyzes a single ATP-independent incision immediately 5' to the UV photoproduct and generates termini containing 3' hydroxyl and 5' phosphoryl groups. Based on these properties, we propose that SPDE may function in a DNA repair capacity, representing the initial recognition/cleavage step of a DNA excision repair pathway.
Bowman
K K
KK
Department of Biochemistry, Emory University School of Medicine, Rollins Research Center, Atlanta, GA 30322.
Sidik
K
K
Smith
C A
CA
Taylor
J S
JS
Doetsch
P W
PW
Freyer
G A
GA
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D008969:Molecular Sequence Data; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology*; D029702:Schizosaccharomyces pombe Proteins; D013379:Substrate Specificity; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D029702:Schizosaccharomyces pombe Proteins; D000255:Adenosine Triphosphate; D004247:DNA; D004706:Endodeoxyribonucleases; C105073:uve1 protein, S pombe