A rapid spectrophotometric blood cyanide determination applicable to emergency toxicology.
Cyanide determination in whole blood can be performed by spectrophotometry after using diffusion coupled with coloration by hydroxocobalamin in a Conway dish. The technique may be accelerated by the use of a heating sheet at 45 degrees C. The method proved to be specific, sensitive, and fast, thus permitting measurements in emergency situations.
Laforge
M
M
Laboratoire de Biochime-Toxicologie, Hôpital Fernand Widal, Paris, France.
Buneaux
F
F
Houeto
P
P
Bourgeois
F
F
Bourdon
R
R
Levillain
P
P
D003486:Cyanides / Q000097:blood*; D004058:Diffusion; D004630:Emergencies; D006801:Humans; D006856:Hydrogen Cyanide / Q000097:blood; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015208:Smoke Inhalation Injury / Q000097:blood; D012907:Smoking / Q000097:blood; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014805:Vitamin B 12 / Q000097:blood
D003486:Cyanides; D007202:Indicators and Reagents; D006856:Hydrogen Cyanide; D014805:Vitamin B 12