Water diffusivity in porcine stratum corneum measured by a thermal gravimetric analysis technique.
Water is a natural constituent of the stratum corneum (sc) affecting its plasticity and modulating its barrier function. Diffusion coefficients (D) were calculated by measuring the desorption rates of water from porcine sc and dermis samples by a thermal gravimetric analysis (TGA) technique at isothermal conditions in the range 30-80 degrees C. Water-loaded samples were exposed to a flow of dry air, and the change of weight and of temperature were monitored with time. Distinct abrupt decreases in rate of desorption marked three different phases of water in the sc, designated as free, bulk, and bound water. Concomitant with the sharp change in evaporation rate, an increase in temperature was observed, in accordance with the absorption of heat accompanied with the water desorption process. Desorption curves were plotted against time and optimized. Values of D were estimated from the ratio of the evaporated water to the initial sc water content, as a function of the square root of time. The "initial slope" and the t1/2 (time for which Mt/Mo = 0.5, where Mo is the equilibrium amount of water absorbed in the membrane and Mt is the amount of water released by the membrane in a time t) methods gave similar results. The water D values of sc at 30 degrees C calculated by the two methods were 3.3 +/- 0.6 x 10(-10) and 2.7 +/- 0.8 x 10(-10) cm2/s, respectively. These values were about two orders of magnitude lower than the calculated D value for water in the dermis.(ABSTRACT TRUNCATED AT 250 WORDS)
Liron
Z
Z
Israel Institute for Biological Research, Ness Ziona.
Wright
R L
RL
McDougal
J N
JN
D000818:Animals; D004058:Diffusion; D066298:In Vitro Techniques; D007700:Kinetics; D012867:Skin / Q000737:chemistry*; D012869:Skin Absorption; D013552:Swine; D013818:Thermogravimetry; D014867:Water / Q000737:chemistry
D014867:Water