Comparative study on the procoagulant from the venom of Australian brown snakes (Elapidae; Pseudonaja spp.).
The procoagulant was isolated from the venom of four clinically significant species of brown snake; Pseudonaja affinis, P. inframacula, P. nuchalis, and P. textilis. HPLC elution profiles and PAGE showed a high degree of homology between the procoagulants from the four species. Antiserum from the CSL Ltd (Brown Snake Antivenom) produced a single band against all four procoagulants. The specific activity of the procoagulant varied between species, while inhibitory studies indicated that the procoagulants were serine proteases with a sialic acid component which also contributes to the coagulant action.
Williams
V
V
Haematology Department, Adelaide Children's Hospital, North Adelaide, Australia.
White
J
J
Mirtschin
P J
PJ
D000818:Animals; D000997:Antivenins / Q000494:pharmacology; D001315:Australia; D001777:Blood Coagulation / Q000187:drug effects*; D001779:Blood Coagulation Factors / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004546:Elapid Venoms / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000633:toxicity; D017815:Elapidae; D004591:Electrophoresis, Polyacrylamide Gel; D009842:Oligopeptides / Q000494:pharmacology; D011233:Precipitin Tests; D012697:Serine Endopeptidases / Q000302:isolation & purification / Q000494:pharmacology; D013045:Species Specificity
D000997:Antivenins; D001779:Blood Coagulation Factors; D004546:Elapid Venoms; D009842:Oligopeptides; C015608:benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide; D012697:Serine Endopeptidases