Detection of DNA strand breaks in barley roots after gamma-ray irradiation using the alkaline unwinding assay.
The alkaline unwinding assay was applied to measure strand breaks in DNA of intact barley roots after gamma-ray irradiation without extracting DNA from the tissue. The most suitable condition for unwinding was determined to be 0.03 M NaOH containing 0.5 M NaCl for alkaline solution composition, and 15 min at 20 degrees C for unwinding time. Under this condition, DNA strand breaks were shown to be linearly increased with increasing dose, and further be rejoined during the post-irradiation incubation for 6 hr.
Shikazono
N
N
Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo, Japan.
Watanabe
H
H
Tanaka
A
A
Tano
S
S
Hirai
A
A
D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D005720:Gamma Rays / Q000009:adverse effects; D005821:Genetic Techniques; D001467:Hordeum / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D012972:Sodium Hydroxide; D012996:Solutions
D012996:Solutions; D012972:Sodium Hydroxide; D004247:DNA