Endothelin-1 inhibits insulin-stimulated glucose uptake in isolated rat adipocytes.
A single class of high affinity endothelin-1 (ET-1) binding sites with an apparent Kd of 350 pM and a binding capacity of 69,000 sites/cell was found in isolated rat adipocytes. Whereas ET-1 had no effect on basal glucose uptake, it inhibited insulin-stimulated glucose uptake in both a dose- and time-dependent manner. Insulin binding, however, was not altered. Thus, the present study demonstrates the presence of ET-1 receptor in isolated rat adipocytes and the inhibition of insulin-stimulated glucose uptake by ET-1 via a post-receptor mechanism.
Chou
Y C
YC
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.
Perng
J C
JC
Juan
C C
CC
Jang
S Y
SY
Kwok
C F
CF
Chen
W L
WL
Fong
J C
JC
Ho
L T
LT
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D016232:Endothelins / Q000378:metabolism / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D016232:Endothelins; D007457:Iodine Radioisotopes; D005947:Glucose