New toxicity determination method that uses fluorescent assay of Escherichia coli.
We describe a new method that uses a fluorogenic bioassay of the beta-glucuronidase conversion of 4-methylumbelliferyl beta-D-glucuronide (MUG) to 4-methylumbelliferone to evaluate the individual toxic effects on Escherichia coli of Al3+, Cr6+, Hg2+ and Li+. This work was designed to examine the effectiveness of this method to measure the effects of five ionic concentrations of either Al3+, Cr6+, Hg2+ or Li+, on the growth of E. coli in a minimal medium that had MUG as the only source of carbon. This method was simple and fast, and its toxicity detection sensitivity was equal to, or greater than, existing bacterial bioassays. The use of the MUG substrate minimized the danger of interference by bacteria other than E. coli. Evaluations of toxicity in samples of public drinking water proved equally sensitive.
Mariscal
A
A
Department of Preventive Medicine and Public Health, School of Medicine, University of Málaga, Spain.
García
A
A
Carnero
M
M
Gómez
E
E
Fernández-Crehuet
J
J
D000535:Aluminum / Q000633:toxicity; D001681:Biological Assay; D002857:Chromium / Q000633:toxicity; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology; D005456:Fluorescent Dyes / Q000378:metabolism*; D005966:Glucuronidase / Q000032:analysis*; D006923:Hymecromone / Q000031:analogs & derivatives* / Q000378:metabolism; D008094:Lithium / Q000633:toxicity; D008628:Mercury / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000032:analysis*
D005456:Fluorescent Dyes; D014874:Water Pollutants, Chemical; D002857:Chromium; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide; D008094:Lithium; D000535:Aluminum; D005966:Glucuronidase; D008628:Mercury