A study on superoxide dismutase activity of some model compounds.
The synthesis and characteristics of a binuclear ligand N,N,N',N'-tetrakis (2'-benzimidazolyl methyl)-1,4-diethylene amino glycol ether (EGTB) and its series of coordination compounds containing copper(II), iron(III), and manganese(II) with and without exogenous bridging ligand which was imidazolate ion (Im-), bipyridine (bpy), or 1,10-phenanthroline (phen) are reported. Depending on the redox potentials by cyclic voltammetry, the coordination compounds can act as catalysts for the dismutation of superoxide radicals (O2-). The detection of the rate constant of the reaction of superoxide ion with nitroblue tetrazolium (NBT) which is inhibited by superoxide dismutase (SOD) and its model compounds of the EGTB system has been performed by a modified illumination method. The rate constants kQ of the catalytic dismutation have been obtained.
Liao
Z
Z
Department of Chemistry, Huazhong Normal University, Wuhan, P.R. China.
Liu
W
W
Liu
J
J
Jiang
Y
Y
Shi
J
J
Liu
C
C
D003300:Copper / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry; D004578:Electron Spin Resonance Spectroscopy; D005026:Ethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D005609:Free Radicals; D007700:Kinetics; D008345:Manganese / Q000737:chemistry / Q000378:metabolism; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D005026:Ethylene Glycols; D005290:Ferric Compounds; D005609:Free Radicals; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D008345:Manganese; D003300:Copper; C088779:N,N,N',N'-tetrakis(2'-benzimidazolylmethyl)-1,4-diethylene amino glycol ether; D013482:Superoxide Dismutase