[Inhibitory analysis of DNA polymerases from human viruses using modified substrates].
A systematic analysis of DNA polymerase of human herpes simplex type 1 virus, cytomegalovirus, and human type 2 adenovirus with the help of a broad set of modified substrates of these enzymes has been carried out. It revealed compounds capable of inhibiting the DNA synthesis catalyzed both by all three enzymes and DNA polymerase alpha from human placenta. Compounds have been found which effectively and specifically inhibit the DNA synthesis catalyzed by some of the abovementioned enzymes. It has been shown that the molecular mechanism of inhibition consists either in the termination of DNA elongation or in inhibition without incorporation into the growing DNA chain.
Kukhanova
M K
MK
American Cyanamid Company, New York.
Kuznetsova
E V
EV
Kraevskiĭ
A A
AA
O'Hara
B
B
Bekker
J
J
Morin
J
J
Gluzman
Ia
Ia
D000260:Adenoviruses, Human / Q000201:enzymology*; D000818:Animals; D001483:Base Sequence; D002384:Catalysis; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000201:enzymology*; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005260:Female; D018259:Herpesvirus 1, Human / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009036:Moths; D019384:Nucleic Acid Synthesis Inhibitors; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D013728:Terminator Regions, Genetic
D019384:Nucleic Acid Synthesis Inhibitors; D004259:DNA-Directed DNA Polymerase
Ingibitornyĭ analiz DNK-polimeraz virusov cheloveka s pomoshch'iu modifitsirovannykh substratov.