Comparison of the utility of capillary zone electrophoresis and high-performance liquid chromatography in peptide mapping and separation.
Capillary zone electrophoresis (CZE) and high-performance liquid chromatography (HPLC) have been used in the analysis of the primary structure of recombinant human insulin-like growth factor I (rhIGF-I). CZE both complements and supplements HPLC separations. CZE has been used to resolve peaks which co-elute on HPLC, as well as to help establish the identity of tryptic fragments in peptide mapping experiments.
Rudnick
S E
SE
Department of Chemistry, Manhattan College, Riverdale, NY.
Hilser
V J
VJ
Worosila
G D
GD
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry
D010455:Peptides; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I