Comparative effects of piroxicam and esculetin on incidence, proliferation, and cell kinetics of mammary carcinomas induced by 7,12-dimethylbenz[a]anthracene in rats on high- and low-fat diets.
We investigated the effects of piroxicam, a cyclooxygenase inhibitor, and esculetin, a lipoxygenase inhibitor, on 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary carcinogenesis in female rats. Seven days after receiving a 5-mg dose of DMBA, rats were fed either a high-fat (20% soybean oil) or low-fat (0.5% soybean oil) diet. One third of the rats received diets containing 0.01% piroxicam and one third received diets containing 0.03% esculetin. Esculetin significantly inhibited mammary tumorigenesis and tumor proliferation in the rats fed the high-fat and the low-fat diets. Piroxicam had no inhibitory effect. Our findings indicate that DMBA-induced mammary tumorigenesis is affected by lipoxygenase products rather than by cyclooxygenase products.
Kitagawa
H
H
Department of Surgery (II), Kanazawa University Hospital, School of Medicine, Japan.
Noguchi
M
M
D015127:9,10-Dimethyl-1,2-benzanthracene; D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004041:Dietary Fats / Q000008:administration & dosage*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D015994:Incidence; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D010894:Piroxicam / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014468:Umbelliferones / Q000494:pharmacology*
D004041:Dietary Fats; D014468:Umbelliferones; D010894:Piroxicam; D015127:9,10-Dimethyl-1,2-benzanthracene; D001973:Bromodeoxyuridine; C007628:esculetin