title: Non-communicating human and murine carcinoma cells produce alpha 1 gap junction mRNA.
abstract: The gap junctional communication capacity of six human carcinoma-derived tumorigenic cell lines (pancreatic, pharyngeal and cervical), two murine carcinoma-derived tumorigenic cell lines (bladder-derived and Ehrlich ascites) and one monkey non-tumorigenic cell line (kidney epithelium) have been compared by the dye-transfer technique. All the tumorigenic cell lines were communication defective, while the non-tumorigenic cell line was not. Moreover, six of the eight tumorigenic cell lines expressed a gap junction transcript coding for the connexin 43 (alpha 1). Finally, gap junction plaques were not detected between tumorigenic cells by immunofluorescence staining. Consequently, these data suggest that communication defects in tumorigenic cells may result from either abnormally low levels of translation of junction mRNA or alterations in the assembly of junction protein into cell surface plaques, and not from failure to produce junction transcripts. Furthermore, since induction of alpha 1 expression has been associated with dedifferentiation processes, the presence of alpha 1 mRNA might be a characteristic property of certain tumorigenic cells that originate from cells that do not normally express alpha 1 mRNA.
authors.affiliation: Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
authors.forename: M
authors.initials: M
authors.lastname: Mesnil
authors.forename: D
authors.initials: D
authors.lastname: Rideout
authors.forename: N M
authors.initials: NM
authors.lastname: Kumar
authors.forename: N B
authors.initials: NB
authors.lastname: Gilula
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000473:pathology*; D002450:Cell Communication; D017630:Connexins / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D017629:Gap Junctions / Q000502:physiology*; D006801:Humans; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D017630:Connexins; D012333:RNA, Messenger