Platelet-derived growth factor alpha receptor gene expression: isolation and characterization of the promoter and upstream regulatory elements.
Receptors for the platelet-derived growth factors (PDGFs) are expressed conditionally in developing embryos and adult tissues. Aberrant expression of PDGF receptors is a molecular marker for proliferative disorders such as atherosclerosis, myofibrosis, and malignant astrocytoma. We isolated genomic clones that encompass the 5' end of the mouse PDGF alpha receptor mRNA transcript and extend 10 kb into the upstream flanking region of the gene. Using these clones, we constructed a partial genomic map that locates the promoter and transcription start sites of the gene. One of our genomic clones contains cis-acting regulatory elements that drive expression of reporter gene constructs selectively in cells that express PDGF alpha receptors.
Wang
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Dana-Farber Cancer Institute, Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Stiles
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D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology; D005786:Gene Expression Regulation; D051379:Mice; D008807:Mice, Inbred BALB C; D011401:Promoter Regions, Genetic; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic
D017479:Receptors, Platelet-Derived Growth Factor