[Evaluation of a selective medium with dilutions for the isolation of Actinobacillus pleuropneumoniae and Haemophilus spp. in pigs].
The aim of the present research was to evaluate the efficiency of a method used to enhance the isolation of Actinobacillus pleuropneumoniae and Haemophilus spp. 134 samples of pneumonic lungs of swine were directly grown in blood agar medium, 120 of these samples were simultaneously processed by a dilution method inoculating then into a selective and enriched broth (1% poly-enriched, 5% yeast extract. 5 micrograms/ml bacitracin, 1 microgram/ml lincomycin and 1 microgram/ml crystal violet). The dilution method proved to be more efficient than the direct one.
Rosado-Vallado
M
M
Unidad de Posgrado e Investigación, Facultad de Química, Universidad Autónoma de Yucatán, Merida, Mexico.
Quintero-Mármol
E
E
Garcilazo
J A
JA
D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000302:isolation & purification*; D003470:Culture Media; D006190:Haemophilus / Q000302:isolation & purification*; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary*; D011014:Pneumonia / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D003470:Culture Media
Evaluación de un medio selectivo con diluciones para el aislamiento de Actinobacillus pleuropneumoniae y Haemophilus spp. en cerdos.