Unique change of pancreastatin-like immunoreactivity in cerebrospinal fluid by aging.
Using a specific antiserum for the C-terminal glycine amide region of human pancreastatin (PST), pancreastatin-like immunoreactivity (PST-LI) was measured in cerebrospinal fluid (CSF) from 447 subjects (368 +/- 10.8 pmol/l, mean +/- S.E.M.) free from endocrine diseases. The CSF contents of PST-LI showed a mountain-shape type change which peaked at 40 years of age. The highest concentration was found in the group of ages 40-49 years old (412 +/- 22.9 pmol/l) and the lowest concentration was found in the group of ages 80-89 years old (293.2 +/- 45.2 pmol/l) among various age groups. Gel chromatographic examination revealed the presence of two major forms (MW 13,500 and 5,400) of PST-LI in CSF. Because of the character of this antibody, the large molecular form is possibly an N-terminally elongated PST and the other may be PST-52. This may be the first report on the unique age-related change of PST concentration in CSF.
Sekiya
K
K
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Nakao
R
R
Takayanagi
R
R
Haji
M
M
Kurose
S
S
Oyama
M
M
Ohashi
M
M
Tateishi
K
K
Funakoshi
A
A
Akazawa
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000134:cerebrospinal fluid*; D002850:Chromatography, Gel; D053379:Chromogranin A; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010187:Pancreatic Hormones / Q000134:cerebrospinal fluid* / Q000737:chemistry / Q000276:immunology; D011863:Radioimmunoassay
D053379:Chromogranin A; D010187:Pancreatic Hormones; C050836:pancreastatin