title: Induction of a calbindin-D9K-like protein in avian muscle cells by 1,25-dihydroxy-vitamin D3.
abstract: 1,25-dihydroxy-vitamin D3 induces the synthesis of a 9 kDa calcium binding protein in chick embryo myoblasts. This work revealed comigration of the myoblast protein with rat calbindin-D9K in SDS-polyacrylamide gels. Western-blot analysis with a specific calbindin-D9K antibody showed the presence of an immunoreactive protein of 9 kDa in chick tissues. In addition, higher levels of myoblast mRNA hybridizing to a specific cDNA probe for rat calbindin-D9K were detected in response to 1,25-dihydroxy-vitamin D3. Northern hybridization analysis showed that the increase was related to a single 550 nucleotide mRNA species. These results provide the first evidence on the presence of calbindin-D9K or a closely related protein in avian tissues and its inducible expression in muscle by 1,25-dihydroxy-vitamin D3.
authors.affiliation: Departamento de Biologia, Universidad Nacional de Sur, Bahia Blanca, Argentina.
authors.forename: D
authors.initials: D
authors.lastname: Lila
authors.forename: Z
authors.initials: Z
authors.lastname: Susana
authors.forename: B
authors.initials: B
authors.lastname: Ricardo
mesh_terms: D000818:Animals; D064026:Calbindins; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis*; D014158:Transcription, Genetic
chemical_list: D064026:Calbindins; D064030:S100 Calcium Binding Protein G; C576508:S100g protein, rat; D002117:Calcitriol