Infectivity titration of the fast-replicating and cytopathic hepatitis A virus strain HM175A.2 by an in situ enzyme immunoassay.
A simple, rapid and objective infectivity assay based on an in situ enzyme immunoassay (EIA) was developed for the fast-growing and cytopathic cell culture-adapted hepatitis A virus (HAV) strain HM175A.2. Infectivity titration by EIA correlated well with titration by cytopathic effects. The reliability of this assay was demonstrated by close agreement in virus infectivity titers among different assays of the same virus aliquot and between assays of different virus aliquots. HAV infected cell cultures after fixation could be stored for up to 1 week before testing without decline in virus titer.
Yap
K L
KL
Department of Biomedical Sciences, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur.
Lam
S K
SK
D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D006506:Hepatitis A / Q000382:microbiology* / Q000473:pathology; D035921:Hepatitis A Antigens; D006507:Hepatovirus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D015588:Observer Variation; D015203:Reproducibility of Results; D014021:Tissue Preservation
D000956:Antigens, Viral; D035921:Hepatitis A Antigens