title: Negative regulation of Sp1 trans-activation is correlated with the binding of cellular proteins to the amino terminus of the Sp1 trans-activation domain.
abstract: Sp1 is a well characterized and ubiquitously expressed transcription factor that regulates the constitutive and induced expression of a variety of mammalian genes. It is unclear whether Sp1 activity is regulated in vivo; the mechanism by which Sp1 interacts with the basal transcription complex has not been firmly established. We report the identification of a ubiquitously expressed and evolutionarily conserved nuclear protein, p74, that specifically binds Sp1 in vivo and in vitro. p74 interacts with several portions of the Sp1 trans-activation domain in vitro, and we correlate the binding of p74 to the amino-terminal serine/threonine-rich subdomain of Sp1 with the inhibition of Sp1-mediated transcription in vivo.
authors.affiliation: Department of Molecular Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: Y
authors.initials: Y
authors.lastname: Murata
authors.forename: H G
authors.initials: HG
authors.lastname: Kim
authors.forename: K T
authors.initials: KT
authors.lastname: Rogers
authors.forename: A J
authors.initials: AJ
authors.lastname: Udvadia
authors.forename: J M
authors.initials: JM
authors.lastname: Horowitz
mesh_terms: D001665:Binding Sites; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D016329:Sp1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D016329:Sp1 Transcription Factor; D014157:Transcription Factors