[Construction of the genomic library for cyanobacteria Synechocystis sp. PCC 6803 using a cosmid].
The gene library of the phototrophic cyanobacterium Synechocystis PCC6803 was constructed in the cosmid vector Lorist6. About -3 x 10(3) recombinant clones were obtained, of which 760 were selected for further work. Each clone was kept in an individual well of a microtiter plate. The clone grids obtained were hybridized with two DNA probes, which contained the ndhE and dfr genes of Synechocystis PCC6803. Two groups of probe-overlapping cosmids (11 and 8 clones respectively), containing insertions averaging of 39 kb, were isolated. The capacity of the gene library is nine equivalents of the Synechocystis PCC6803 genome and its representativity exceeds 99%.
Shestopalov
V I
VI
Nashchokina
O O
OO
Shestakov
S V
SV
Iankovskiĭ
N K
NK
D003001:Cloning, Molecular; D003360:Cosmids; D000458:Cyanobacteria / Q000235:genetics*; D004269:DNA, Bacterial; D016680:Genome, Bacterial
D004269:DNA, Bacterial
Konstruirovanie kosmidnoĭ biblioteki genov tsianobakterii Synechocystis sp. PCC 6803.