Manipulating blood T cells and B cells from squirrel monkeys: some technical considerations.
The squirrel monkey Saimiri sciureus is an experimental host for a range of human pathogens, and for the assessment of vaccine candidate antigens and vaccine strategies. This experimental host is thus particularly suitable for the follow-up of humoral responses. To understand some of the mechanisms that underlie the defense against experimental pathogens, there is a need of basic knowledge on cellular immune effectors also. The authors report here their experience in characterizing squirrel monkey blood T and B lymphocytes, and in studying in vitro induced activation and proliferation of T and B cells. Particular emphasis is given to the in vitro differentiation of squirrel monkey B cells into immunoglobulin secreting cells, with respect to Plasmodium falciparum antigens.
Garraud
O
O
Laboratoire d'Immunologie Parasitaire, Institut Pasteur de Guyane, French Guiana.
Perraut
R
R
Gysin
J
J
Behr
C
C
Dubois
P
P
Bonnemains
B
B
Jouin
H
H
Michel
J C
JC
Pereira da Silva
L
L
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D010963:Plasmodium falciparum / Q000276:immunology; D012453:Saimiri / Q000097:blood / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014168:Transferrin / Q000502:physiology
D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D014168:Transferrin