NADPH oxidase is not essential for low density lipoprotein oxidation by human monocyte-derived macrophages.
NADPH oxidase has been reported to be involved in low density lipoprotein (LDL) oxidation by monocytes. We have investigated the ability of monocyte-derived macrophages from four chronic granulomatous disease patients, which lack NADPH oxidase, to oxidise LDL. All the cells oxidised LDL to significantly increase its uptake by mouse macrophages. We conclude therefore that NADPH oxidase is not essential for LDL oxidation by macrophages.
Wilkins
G M
GM
Department of Biochemistry & Physiology, University of Reading, Whiteknights, Berkshire, U.K.
Segal
A W
AW
Leake
D S
DS
D000818:Animals; D005260:Female; D006105:Granulomatous Disease, Chronic / Q000097:blood*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000201:enzymology / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D019255:NADPH Oxidases; D010084:Oxidation-Reduction
D008077:Lipoproteins, LDL; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases