title: A patch-clamp study on a novel gamma-aminobutyric acid activated chloride channel of crayfish deep extensor abdominal muscle.
abstract: The patch-clamp technique was used to study the effect of rapid pulses of gamma-aminobutyric acid (GABA) on excised membrane-patches in the outside-out configuration of the deep extensor abdominal muscle (DEAM) of crayfish. Channel currents reversed at the equilibrium potential of Cl- and were blocked by picrotoxin. Rare channel openings were elicited by 0.1 mM GABA, and a saturating open probability of 0.9 was reached with 10 nM GABA. The investigated channel was only sensitive to GABA and is different from a previously described GABAergic channel in crayfish that is in addition sensitive to acetylcholine and glutamate and shows three subconductance states.
authors.affiliation: Physiologisches Institute der Technischen Universität München, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Adelsberger
authors.forename: N
authors.initials: N
authors.lastname: von Beckerath
authors.forename: C
authors.initials: C
authors.lastname: Franke
authors.forename: J
authors.initials: J
authors.lastname: Dudel
mesh_terms: D000009:Abdominal Muscles / Q000378:metabolism*; D000818:Animals; D003400:Astacoidea / Q000378:metabolism*; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000502:physiology*; D004594:Electrophysiology; D010852:Picrotoxin / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D018118:Chloride Channels; D010852:Picrotoxin; D005680:gamma-Aminobutyric Acid