title: [Transferrable broad-spectrum beta-lactam resistance in Klebsiella pneumoniae R-3455].
abstract: Bacterial resistance to antimicrobial agents is a common problem observed in hospitals. We characterized a clinical isolate of Klebsiella pneumoniae (R-3455) which was resistant to high concentrations of broad spectrum beta-lactams, aminoglycosides, fluoroquinolones, chloramphenicol and tetracycline. Conjugation experiments showed that the multiresistance could be transferred to Escherichia coli J53-2 receptor strain. The transconjugant X-3455 was resistant to all antibacterials assayed in R-3455, except to fluoroquinolones. We found that both strains R-3455 and X-3455 produced a beta-lactamase which was sensitive to clavulanic acid. Southern hybridization and PCR analysis showed the presence of at least, a TEM type beta-lactamase gene in both strains.
authors.affiliation: Instituto Nacional de Salud Pública, Departamento de Bacteriología, Cuernavaca, Morelos, Mexico.
authors.forename: M C
authors.initials: MC
authors.lastname: Conde-Bonfil
authors.forename: J
authors.initials: J
authors.lastname: Silva
authors.forename: C
authors.initials: C
authors.lastname: Ovando
authors.forename: Z
authors.initials: Z
authors.lastname: Becerra
authors.forename: V
authors.initials: V
authors.lastname: González
authors.forename: Y
authors.initials: Y
authors.lastname: Fuchs
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics; D008969:Molecular Sequence Data; D011815:R Factors; D001618:beta-Lactamases / Q000235:genetics; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D047090:beta-Lactams; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1
vernacular_title: Resistencia transferible de beta-lactámicos de amplio espectro en Klebsiella pneumoniae R-3455.