Regulation of transcription of the cel1 gene in Agaricus bisporus.
Northern analysis showed that accumulation of Agaricus bisporus cel1 mRNA was regulated by two independent mechanisms: (i) induction by cellulose; and (ii) repression by glucose and other sugars. Isolated A. bisporus nuclei were transcriptionally active. Nuclei isolated from cellulose-grown mycelium synthesized six times more cel1 mRNA than nuclei from glucose-grown mycelium. The start point of transcription (tsp) was identified by primer extension and S1 nuclease analysis. Putative glucose-, and cAMP-responsive elements as well as regions with homology to promoter regions of other fungal cellulase genes were detected both upstream and downstream from the tsp of the cel1 gene.
Yagüe
E
E
Division of Life Sciences, King's College London, UK.
Wood
D A
DA
Thurston
C F
CF
D000364:Agaricus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics*; D043366:Cellulose 1,4-beta-Cellobiosidase; D000242:Cyclic AMP / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D000242:Cyclic AMP; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase