Accumulation of adoptively transferred A-NK cells in murine metastases: kinetics and role of interleukin-2.
Infiltration of lung metastases by adoptively transferred A-NK cells was time- and dose-dependent. Infiltration became significant 6 hours after injection and reached a maximum after 16-24 hours. When no exogenous IL-2 was administered to support the injected cells, hardly any A-NK cells infiltrated the tumors. More A-NK cells accumulated in the tumors when high doses of IL-2 were given compared to lower doses. Significantly more A-NK cells accumulated in the tumours when the total dose of IL-2 was given as many small doses at short intervals compared to larger doses given less frequently. Use of IL-2 complexed to Polyethylene-glycol (Peg-IL-2) resulted in a significantly better tumor infiltration by A-NK cells than use of regular IL-2. IL-2 administered in mini-osmotic pumps did not lead to a better infiltration than ip injections of the same amount of IL-2. The route of administration of IL-2 did not seem to influence the degree of infiltration.
Basse
P H
PH
Institute of Medical Microbiology, University of Aarhus, Denmark.
Goldfarb
R H
RH
Herberman
R B
RB
Hokland
M E
ME
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004334:Drug Administration Schedule; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D007700:Kinetics; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology / Q000556:secondary*; D017710:Lymphocyte Transfusion; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008546:Melanoma, Experimental / Q000276:immunology* / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000276:immunology; D013154:Spleen / Q000276:immunology; D014407:Tumor Cells, Cultured
D007376:Interleukin-2