Determination of clenbuterol in cattle, sheep, and swine tissues by electron ionization gas chromatography/mass spectrometry.
A gas chromatographic/mass spectrometric procedure is described for the quantitation and confirmation of clenbuterol residues from cattle, sheep, and swine tissues. After liquid-liquid extraction and derivatization with phosgene in an aqueous pH 10.1 buffer, the cyclic oxazolidone derivative is quantitated with a clenbuterol analogue as internal standard (NAB-760 Cl). Confirmation is accomplished by comparison of ion ratios with those of a pure synthesized standard of clenbuterol oxazolidin-3-one obtained by selected ion monitoring, electron ionization gas chromatography/mass spectrometry on a benchtop instrument. Statistical information based on a series of standard curves for fortified tissues is included to describe method performance. Ion ratio variations were under 15%, and coefficients of variation for spiked tissue standard curves were above 0.997. Recoveries averaged 87.1 +/- 6.6% for liver tissues across all 3 species and 67.1 +/- 3.8% for muscle tissue across all 3 species.
Wilson
R T
RT
U.S. Department of Agriculture, Food Safety and Inspection Service, Midwestern Laboratory, St. Louis, MO 63115.
Groneck
J M
JM
Holland
K P
KP
Henry
A C
AC
D000818:Animals; D002417:Cattle; D002976:Clenbuterol / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008099:Liver / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D012756:Sheep; D013552:Swine
D002976:Clenbuterol