Benzoylation of sugars, polyols and amino acids in biological fluids for high-performance liquid chromatographic analysis.
A precolumn benzoylation for analyzing sugars, polyols and neutral amino acids in biological fluids by high-performance liquid chromatography has been developed, which avoids protein precipitation, drying procedures and the use of pyridine. Derivatization and chromatography can be performed within one hour with a minimum detectable quantity of ca. 1 pmol (signal-to-noise ratio > 2). The derivatization products of glucose, mannitol and neutral amino acids were identified by electrospray mass spectrometry (ES-MS) to be tetra- and pentabenzoyl glucose, penta- and hexabenzoyl mannitol and 2-phenyl-5-benzoyloxyoxazoles, respectively.
Oehlke
J
J
Institute of Molecular Pharmacology, Berlin, Germany.
Brudel
M
M
Blasig
I E
IE
D000596:Amino Acids / Q000097:blood*; D000818:Animals; D002241:Carbohydrates / Q000097:blood*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D011108:Polymers / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D002241:Carbohydrates; D011108:Polymers; C024617:polyol