Sequence and expression of the todGIH genes involved in the last three steps of toluene degradation by Pseudomonas putida F1.
The todFC1C2BADE gene cluster in Pseudomonas putida F1 encodes enzymes for the first four steps of toluene degradation, leading to the formation of 2-hydroxypenta-2,4-dienoate (HPD). Here, we report the nucleotide (nt) sequence and expression of the remaining three genes of the tod pathway, downstream from todE and arranged in the order, todGIH. The deduced amino acid (aa) sequences of TodG [HPD hydratase (268 aa)], TodH [4-hydroxy-2-oxovalerate (HO) aldolase (352 aa)] and TodI [acylating aldehyde (AA) dehydrogenase (316 aa)] are compared with the isofunctional proteins present in the meta-cleavage pathways of other bacteria. New sequence motifs are identified. The highly conserved TodH and TodI sequences are potentially useful DNA probes for biomonitoring purposes.
Lau
P C
PC
Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec.
Bergeron
H
H
Labbé
D
D
Wang
Y
Y
Brousseau
R
R
Gibson
D T
DT
D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism; D014050:Toluene / Q000378:metabolism*
D014050:Toluene; D000445:Aldehyde Oxidoreductases; C027014:acetaldehyde dehydrogenase (acylating); C021417:4-hydroxy-2-oxovalerate aldolase; D007652:Oxo-Acid-Lyases; D006836:Hydro-Lyases; C061677:2-oxopent-4-enoate hydratase