Two molecular forms of peptide YY (PYY) are abundant in human blood: characterization of a radioimmunoassay recognizing PYY 1-36 and PYY 3-36.
Two endogenous forms of PYY are abundant in man and dog, PYY 1-36 (PYY-I) and PYY 3-36 (PYY-II). PYY-II is a major molecular form of PYY in human colon, but it is not known, whether PYY-II is also released into the circulating blood. Several radioimmunoassays for measuring PYY-I in plasma have been developed, but it has not been reported, whether they equally detect PYY-II. We characterize a radioimmunoassay for measuring PYY in human plasma which equally recognizes PYY-I and PYY-II. Using this radioimmunoassay and reversed phase HPLC we demonstrate the existence of two forms of PYY in human blood, coeluting with synthetic PYY-I and PYY-II.
Grandt
D
D
Department of Gastroenterology, University of Essen, Germany.
Schimiczek
M
M
Beglinger
C
C
Layer
P
P
Goebell
H
H
Eysselein
V E
VE
Reeve
J R
JR
D000328:Adult; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004285:Dogs; D006801:Humans; D010446:Peptide Fragments; D019894:Peptide YY; D010455:Peptides / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D012680:Sensitivity and Specificity
D010446:Peptide Fragments; D010455:Peptides; D019894:Peptide YY; C061785:peptide YY (3-36)