Isolation of a poplar and an Arabidopsis thaliana dihydrodipicolinate synthase cDNA clone.
A poplar DHDPS cDNA clone has been isolated by functional rescue of the dapA-deficient AT997 mutant of Escherichia coli. By sequence comparison between the poplar and maize DHDPS cDNAs, two oligonucleotides were designed to perform polymerase chain reaction (PCR) on Arabidopsis thaliana genomic DNA. The PCR fragment was subsequently used to isolate an Arabidopsis DHDPS genomic and cDNA clone.
Vauterin
M
M
Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, St.-Genesius Rode, Belgium.
Jacobs
M
M
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D015698:Genomic Library; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014197:Trees / Q000201:enzymology / Q000235:genetics
D018076:DNA, Complementary; D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase