Polyunsaturated fatty acids reduce pyrogen-induced tissue factor expression in human monocytes.
Endotoxin (LPS) and interleukin-1 beta (IL-1 beta) increased the expression of tissue factor, a membrane-anchored glycoprotein that initiates blood coagulation on the surface of cultured human umbilical vein endothelial cells (HUVEC) and human monocyte/macrophages. On monocyte/macrophages, oleic acid strongly inhibited LPS-induced tissue factor expression, a similar activity also being obtained with regard to the pyrogenic effects of IL-1 beta. Other polyunsaturated fatty acids such as linoleic or linolenic acid also reduced tissue factor expression whereas palmitic acid was ineffective. In contrast, these compounds showed no effect on LPS- or IL-1 beta-induced tissue factor expression in HUVEC when tested at the concentration of 10 microM. These data therefore suggest that the well-recognized antithrombotic and antiatherogenic effect of polyunsaturated fatty acids may in part be mediated through an inhibition of tissue factor expression in monocyte/macrophages.
Lalé
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Sanofi Recherche, Toulouse, France.
Herbert
J M
JM
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D015870:Gene Expression; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D011749:Pyrogens / Q000037:antagonists & inhibitors*; D013925:Thromboplastin / Q000378:metabolism*
D005231:Fatty Acids, Unsaturated; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011749:Pyrogens; D013925:Thromboplastin