High-performance liquid chromatographic assay for ibuprofen in whole blood using solid-phase extraction.
A precise, accurate, reproducible one-step method for the high-performance chromatographic determination of ibuprofen in whole blood is described. Samples were, after haemolysis, prepared by solid-phase extraction. Analyses were performed using reversed-phase chromatography on a Separon SGX C18 column with a mobile phase of methanol-water (pH 3) and ultraviolet detection at 222 nm. The method was used for pharmacokinetic studies in rabbits.
Sochor
J
J
Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic.
Klimes
J
J
Zahradnícek
M
M
Sedlácek
J
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007052:Ibuprofen / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D007202:Indicators and Reagents; D007213:Indomethacin / Q000097:blood; D007275:Injections, Intravenous; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D007052:Ibuprofen; D007213:Indomethacin