The mechanism of transcription termination by RNA polymerase I.
Eukaryotic ribosomal gene transcription units are bordered at their 3' ends by short DNA sequences which specify site-specific termination by RNA polymerase I (polI). PolI terminators from yeast through to mammals appear to follow similar rules: they contain a site for a sequence-specific DNA-binding protein; they function only in one orientation; 3' ends are formed upstream of the binding site; and 5' flanking sequences influence the position and efficiency of 3' end formation. Recent progress in understanding the mechanism of RNA chain elongation by other polymerases suggests a model for polI termination in which termination is seen as one of the several outcomes possible when a polymerase encounters a pause site.
Reeder
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Fred Hutchinson Cancer Research Center, Basic Sciences Division, Seattle, Washington 98104.
Lang
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D000818:Animals; D001001:Anura / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D005656:Fungal Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D012318:RNA Polymerase I / Q000378:metabolism*; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D012318:RNA Polymerase I