PEBP2--a modulator of polyoma DNA replication.
Previously, we have shown that integrated copies of polyoma DNA can be induced to replicate in rat fibroblasts (H3 cells) exposed to a DNA-damaging agent. In the current study, we demonstrate that UV-irradiation of mouse fibroblasts (WOP cells), transiently transfected with polyoma DNA, results in repression of polyoma replication. Cotransfection of oligomers representing wild-type but not mutated forms of the PEBP2 target sequence restored levels of viral replication indicating a role of PEBP2 binding proteins in mediating this effect. DNA-binding assays revealed that a different subset of complexes was formed with the PEBP2 target sequence when nuclear proteins from sham and UV-irradiated WOP and H3 cells were compared, suggesting that the activities of PEBP2 binding proteins are differentially regulated upon UV-irradiation in these two cell types. The ability of PEBP2 to modulate polyoma replication following UV-irradiation in WOP cells suggests a potential role of PEBP2 proteins in the cellular response to DNA damage.
Rutberg
S E
SE
Molecular Carcinogenesis Program, American Health Foundation, Valhalla, NY 10595.
Dolan
L R
LR
Ronai
Z
Z
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit; D050658:Core Binding Factor beta Subunit; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000235:genetics* / Q000502:physiology; D051381:Rats; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000502:physiology*; D014466:Ultraviolet Rays; D014779:Virus Replication
C493693:Cbfb protein, mouse; D050659:Core Binding Factor Alpha 1 Subunit; D050658:Core Binding Factor beta Subunit; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C493716:Runx2 protein, mouse; C493717:Runx2 protein, rat; D050656:Transcription Factor AP-2; D014157:Transcription Factors