A T7 promoter vector with a transcriptional terminator for stringent expression of foreign genes.
An expression vector, pKK233-2 [Amann and Brosius, Gene 40 (1985) 183-190], was modified by replacing the trc promoter with the T7 phi 10 promoter and introducing a strong transcriptional terminator upstream from this promoter. This modification successfully suppressed background transcription and resulted in strict dependence upon induction.
Nishihara
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T
Department of Biochemistry, Kawasaki Medical School, Okayama, Japan.
Iwabuchi
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T
Nohno
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T
D017123:Bacteriophage T7 / Q000235:genetics*; D001483:Base Sequence; D004274:DNA, Recombinant; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D004274:DNA, Recombinant