Nerve growth factor induces trkA mRNA expression in cultured basal forebrain cholinergic neurons from 17-day fetal rats.
We first examined the basal forebrain tissues for developmental changes in expression of the high-affinity NGF receptor (trkA) gene. Our reverse transcriptase polymerase chain reaction (RT-PCR) analysis showed that trkA mRNA is present in those tissues of postnatal rats, but not in those of fetal rats. Then we determined the effect of NGF on trkA gene expression in serum-free cultures of basal forebrain neurons from 17-day fetal rats. NGF was found to induce trkA mRNA 36 h after the addition of NGF, while no trkA mRNA was detected in the absence of NGF.
Kojima
M
M
Division of Protein Biosynthesis, Institute for Protein Research, Osaka University, Japan.
Ikeuchi
T
T
Hatanaka
H
H
D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D016548:Prosencephalon / Q000187:drug effects / Q000196:embryology / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
D009414:Nerve Growth Factors; D012333:RNA, Messenger; D002795:Choline O-Acetyltransferase; D020794:Receptor Protein-Tyrosine Kinases