Evaluation of photoaffinity labeled sites of bacteriorhodopsin using molecular modeling.
Three successful photoaffinity labeling experiments of bacteriorhodopsin (bR) have been reported that used photoactivatible analogs of retinal to label the retinal binding site of the protein. Using molecular modeling techniques, the information about the retinal binding site derived from these studies is compared to the retinal binding site as defined by Henderson et al. (1990) using electron diffraction data. This comparison suggests some limitations to the use of photoaffinity labeling experiments for the determination of high resolution structural information.
Beischel
C J
CJ
Department of Pharmacology, Medical University of South Carolina, Charleston 29425.
Gray
K
K
Hazard
E S
ES
Knapp
D R
DR
Crouch
R K
RK
D000345:Affinity Labels; D000595:Amino Acid Sequence; D001436:Bacteriorhodopsins / Q000737:chemistry*; D005069:Evaluation Studies as Topic; D008027:Light; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017433:Protein Structure, Secondary
D000345:Affinity Labels; D001436:Bacteriorhodopsins