Monoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake).
A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a proteoglycan fraction derived from Grifola frondosa (Maitake mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G2b), designated MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not with dextran, pullulan, mannan and xylan. Immunohistochemistry (ABC-GO method) showed that MAb MPG2 reacted with lysosomal proteoglycan and (1-->6)-beta-branched laminaritriose taken up by rabbit peritoneal macrophages. These results suggest that this MAb may recognize mainly (1-->3)-beta-D-glucan, and may be useful for determining the immunological properties of Grifola frondosa-derived proteoglycan.
Hirata
A
A
Research Laboratory, Taito Co., Ltd., Kobe, Japan.
Adachi
Y
Y
Itoh
W
W
Komoda
M
M
Tabata
K
K
Sugawara
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001487:Basidiomycota; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005936:Glucans / Q000276:immunology*; D007114:Immunization; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011134:Polysaccharides / Q000276:immunology; D011509:Proteoglycans / Q000276:immunology*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D005936:Glucans; D011134:Polysaccharides; D011509:Proteoglycans