title: DNA-ploidy, G2M-fractions and prognosis of stages B and C prostate carcinoma.
abstract: Paraffin embedded tissue of 49 stage C and 27 stage B prostate adenocarcinomas was investigated by flow cytometry. All patients were treated by radical prostatectomy with pelvic lymphadenectomy and followed up for 5-10 years. The tumour was separated from the benign tissue to increase the proportion of tumour cells. Ten stage C and seven stage B carcinomas had to be excluded because of poor fixation. Six of the 39 (15%) stage C and 1/20 (5%) stage B carcinomas were aneuploid. Cell cycle analysis was done with correction for sliced nuclei and background subtraction. The threshold between carcinomas with low and with increased ("tetraploid") G2M-fraction was determined by comparing carcinomas with and without tumour progression. Sixty-seven percent of the patients with non-euploid stage C carcinomas and 11% of those with euploid carcinomas suffered from tumour progression (P < 0.01). The respective values for the stage B carcinomas were 67% and 6% (P < 0.01). These results demonstrate the strong prognostic impact of DNA-ploidy and G2M-fractions for each individual patient.
authors.affiliation: Institute of Pathology, University of Würzburg, Germany.
authors.forename: J G
authors.initials: JG
authors.lastname: Müller
authors.forename: S
authors.initials: S
authors.lastname: Demel
authors.forename: M P
authors.initials: MP
authors.lastname: Wirth
authors.forename: A
authors.initials: A
authors.lastname: Manseck
authors.forename: H G
authors.initials: HG
authors.lastname: Frohmüller
authors.forename: H A
authors.initials: HA
authors.lastname: Müller
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics; D016195:G2 Phase; D006801:Humans; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm