Susceptibility testing of Dientamoeba fragilis ATCC 30948 with iodoquinol, paromomycin, tetracycline, and metronidazole.
Susceptibility testing was performed on Dientamoeba fragilis ATCC 30948 in a dixenic culture with Klebsiella pneumoniae and Bacteroides vulgatus. D. fragilis was cocultured with the bacteria in TYGM-9 medium (ATCC medium 1171). The activities of antiparasitic drugs were assessed by counting viable D. fragilis trophozoites with a hemacytometer by trypan blue exclusion. The minimal amebicidal concentrations of the following four drugs were determined: iodoquinol at 128 micrograms/ml, paromomycin at 16 micrograms/ml, tetracycline (questionably) at 32 micrograms/ml, and metronidazole at 32 micrograms/ml.
Chan
F T
FT
Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, Ottawa, Canada.
Guan
M X
MX
Mackenzie
A M
AM
Diaz-Mitoma
F
F
D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D001439:Bacteroides / Q000187:drug effects; D003470:Culture Media; D004029:Dientamoeba / Q000187:drug effects*; D004103:Iodoquinol / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008795:Metronidazole / Q000494:pharmacology; D010303:Paromomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology
D000981:Antiprotozoal Agents; D003470:Culture Media; D008795:Metronidazole; D010303:Paromomycin; D004103:Iodoquinol; D013752:Tetracycline