[Design of the generalized consensus matrix for recognizing sites of 3'-terminal processing of vertebrate pre-mRNA].
Using a set of sequences of 63 cleavage/polyadenylation sites of vertebrate pre-mRNA, a generalized consensus matrix was constructed. The elements of the matrix were the absolute frequencies of oligonucleotides of length l at the i-th position of the sites (the cleavage point of each site was assigned the same position number). To recognize a polyadenylation site in a nucleotide sequence, a multiplicative measure was obtained using the elements of the generalized consensus matrix as weighting factors. For any omega-long fragment of a nucleotide sequence, the estimated value of the functional mu was compared with the threshold value mu. Based on the results obtained, we determined whether or not the given fragment is a processing site.
Kondrakhin
Iu V
IuV
Shamin
V V
VV
D000818:Animals; D001665:Binding Sites; D006801:Humans; D006868:Hydrolysis; D011061:Poly A / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D013698:Templates, Genetic
D012322:RNA Precursors; D012333:RNA, Messenger; D011061:Poly A
Postroenie matritsy obobshchennogo konsensusa dlia raspoznavaniia saĭtov 3'-kontsevogo protsessinga pre-mRNK pozvonochnykh.