The different behavior of diphtheria toxin, modeccin and ricin in HeLa cells infected with Trypanosoma cruzi.
We have studied the action of diphtheria toxin, modeccin and ricin on HeLa cells infected by Trypanosoma cruzi. Parasitized HeLa cells were resistant to diphtheria toxin and modeccin, whereas non-parasitized cells from the same cultures and control cultures showed cytopathological alterations. Protein synthesis, assayed by the incorporation of labelled methionine, diminished in toxin-treated control cultures but remained unaltered in the infected ones, compared to synthesis by untreated infected cells. Ricin, on the other hand, is a toxin that enters the cytoplasm by endocytosis. It has greater cytopathological effects in parasitized cells than in non-parasitized ones from the same cultures or uninfected control cells. Protein synthesis was inhibited in infected cultures treated with ricin.
Osuna
A
A
Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Spain.
Rodriguez-Cabezas
N
N
Gamarro
F
F
Mascaro
C
C
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004167:Diphtheria Toxin / Q000633:toxicity*; D004351:Drug Resistance; D006367:HeLa Cells / Q000187:drug effects / Q000469:parasitology; D006801:Humans; D037102:Lectins / Q000633:toxicity*; D037121:Plant Lectins; D014176:Protein Biosynthesis; D054790:Ribosome Inactivating Proteins, Type 2; D012276:Ricin / Q000633:toxicity*; D014118:Toxins, Biological / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000502:physiology*
D004167:Diphtheria Toxin; D037102:Lectins; D037121:Plant Lectins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C016004:modeccin; D012276:Ricin