title: Comparison of two techniques for measurement of in vitro killing kinetics of five antibiotics against Pseudomonas aeruginosa.
abstract: The in vitro activity of ticarcillin, imipenem, gentamicin, amikacin and ciprofloxacin against six Pseudomonas aeruginosa isolates was evaluated by two time-killing curve methods: the conventional broth technique, and another method previously described which employs a transfer filter membrane. The patterns of the killing curves obtained over a 5 h period with the two techniques were similar. In contrast to the results obtained for beta-lactam agents, the reduction of inoculum was great and increased with the concentration for aminoglycosides and ciprofloxacin. After 5 h, regrowth of the six strains tested was observed in broth with all antibiotics, whereas a bactericidal effect was observed over 24 h with the filter membrane method. Further studies are warranted to determine the reasons for this difference.
authors.affiliation: Department of Bacteriology, Faculty of Medicine, Saint-Antoine, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Lesage
authors.forename: F
authors.initials: F
authors.lastname: Delisle
authors.forename: G
authors.initials: G
authors.lastname: Richard
authors.forename: B
authors.initials: B
authors.lastname: Burghoffer
authors.forename: D
authors.initials: D
authors.lastname: Le Cunff
authors.forename: J C
authors.initials: JC
authors.lastname: Petit
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D002939:Ciprofloxacin / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D015378:Imipenem / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D013982:Ticarcillin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D002939:Ciprofloxacin; D015378:Imipenem; D000583:Amikacin; D013982:Ticarcillin