Measurement of urinary endothelin-1-like immunoreactivity and comparison with other urinary parameters in patients with primary vesicoureteral reflux. A preliminary report.
By using a radioimmunoassay specific for endothelin-1 (ET-1), we measured urinary excretion of ET-1-like immunoreactivity in 63 spot urine samples of 48 patients with primary vesicoureteral reflux (VUR). Urinary excretion of N-acetyl-beta-D-glucosaminidase (NAG), beta 2-microglobulin (beta 2-MG), microalbumin (Alb), and creatinine (Cr) were also measured. There was no significant correlation in any pairs of ET-1 and NAG, ET-1 and beta 2-MG or ET-1 and Alb. In patients with grade 2, grade 3, and grade 4 VUR, urinary ET-1/Cr was significantly higher than normal (p < 0.05). Comparing the grade of reflux according to the International Classification with urinary ET-1/Cr, the ratio of more than normal urinary ET-1/Cr increased in proportion to the grade of reflux, but it conversely decreased in grade 5. In conclusion, urinary ET-1 may be an indicator of distal renal tubular or collecting duct injury in patients with primary VUR.
Takeda
M
M
Department of Urology, Niigata University, School of Medicine, Japan.
Komeyama
T
T
Katayama
Y
Y
Tsutsui
T
T
Mizusawa
T
T
Takahashi
H
H
Hatano
A
A
Obara
K
K
Sato
S
S
D000118:Acetylglucosaminidase / Q000652:urine; D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D003404:Creatinine / Q000652:urine; D016232:Endothelins / Q000652:urine*; D005260:Female; D006801:Humans; D007223:Infant; D007674:Kidney Diseases / Q000175:diagnosis* / Q000209:etiology; D008297:Male; D008875:Middle Aged; D014718:Vesico-Ureteral Reflux / Q000150:complications*; D001613:beta 2-Microglobulin / Q000652:urine
D015415:Biomarkers; D016232:Endothelins; D001613:beta 2-Microglobulin; D003404:Creatinine; D000118:Acetylglucosaminidase