title: Cloning of a VLP-protein coding gene from a parasitoid wasp Venturia canescens.
abstract: Virus-like particles (VLPs) that are bound to the egg surface of a parasitic wasp Venturia canescens are void of nucleic acid and unable to infect host tissue but instead provide a passive protection against the host's immune recognition. To investigate evolutionary and functional properties of Venturia particles, we isolated cDNAs coding for a VLP-protein by screening an expression library of the wasp, using antibodies against purified VLPs. The corresponding coding DNA is present in the wasp genome as a single-copy gene.
authors.affiliation: Institut für Biologie III, Freiburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Theopold
authors.forename: E
authors.initials: E
authors.lastname: Krause
authors.forename: O
authors.initials: O
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D005091:Exons; D015723:Gene Library; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010447:Peptide Hydrolases; D011506:Proteins / Q000235:genetics*; D018626:Retroelements; D014863:Wasps / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D011506:Proteins; D018626:Retroelements; D004247:DNA; D010447:Peptide Hydrolases; C063089:Copia protein, Drosophila