title: Isolation of rotavirus strains from naturally infected piglets.
abstract: Twenty rotavirus strains were isolated in 1991-92 from 60 faecal samples collected from diarrhoeic piglets in the Czech and Slovak Republics. Three isolates were adapted to the growth in the cell line MA-104 and produced cytopathic effect. Rotavirus was demonstrated by immunofluorescence test, electron microscopy, polyacrylamide gel electrophoresis, immunoperoxidase test and ELISA.
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic.
authors.forename: K
authors.initials: K
authors.lastname: Sesták
authors.forename: J
authors.initials: J
authors.lastname: Musilová
mesh_terms: D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D018153:Czech Republic; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000382:microbiology / Q000662:veterinary*; D018154:Slovakia; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D012367:RNA, Viral