Efficient MHC class II-restricted presentation of measles virus to T cells relies on its targeting to its cellular receptor human CD46 and involves an endosomal pathway.
The role of the measles virus (MV) receptor, human CD46, in the uptake of MV and antigen presentation by Major Histocompatibility Complex (MHC) class II molecules was investigated. Expression of CD46 in murine B cells resulted in cells highly efficient in capturing UV-inactivated MV particles and presenting both envelope hemagglutinin H and nucleoprotein N to specific T cell hybridomas. Although MV fuse with the plasma membrane of its target cells, presentation of both MV-H and -N was sensitive to inhibition by chloroquine but was not affected by a tripeptide which prevents virus-cell fusion. Whereas 50 microM of chloroquine was required to inhibit presentation of MV-H, purified H or soluble N, only a two-fold lower concentration was required to inhibit that of MV-N. This shows that some CD46-mediated captured MV particles are endocytosed, then disrupted and processed in an endosome/lysosome compartment.
Gerlier
D
D
I.V.M.C., CNRS-UCBL UMR 30, Faculté Alexis Carrel, France.
Trescol-Biémont
M C
MC
Varior-Krishnan
G
G
Naniche
D
D
Fugier-Vivier
I
I
Rabourdin-Combe
C
C
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology; D015703:Antigens, CD / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology; D002738:Chloroquine / Q000494:pharmacology; D004705:Endocytosis / Q000502:physiology; D006389:Hemagglutinins, Viral / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008247:Lysosomes / Q000378:metabolism; D008459:Measles virus / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000276:immunology; D009842:Oligopeptides / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000276:immunology
D015703:Antigens, CD; C497857:CD46 protein, human; D006389:Hemagglutinins, Viral; D000949:Histocompatibility Antigens Class II; C497859:Mcp protein, mouse; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D009842:Oligopeptides; D014764:Viral Proteins; C032397:hemagglutinin protein G, measles virus; C058314:nucleoprotein, Measles virus; D002738:Chloroquine