title: Growth-arrested dependence of aldolase A L-type mRNA expression in rodent cell lines.
abstract: Two ubiquitous (L- and F-type) and one muscle-specific (M-type) mRNA species are generated by the human aldolase A gene. Despite the high degree of sequence similarities in the promoter region between human and rodents, no L-type mRNA expression has yet been found in the latter. Here we demonstrate that L-type aldolase A mRNA is expressed during the differentiation of mouse myogenic C2.7 and rat oligodendrocyte precursor CEINGE C13 cells. The L-type mRNA expression is increased during differentiation and is associated with cell-growth arrest caused by nocodazole treatment or serum deprivation in C2.7 and CEINGE C13 cells, respectively. The L-type aldolase A mRNA is correctly processed at the L1-L2 junction.
authors.affiliation: Dipartimento Biochimica e Biotecnologie Mediche, Facoltà di Medicina, Università degli studi di Napoli Federico II.
authors.forename: A
authors.initials: A
authors.lastname: Lupo
authors.forename: P
authors.initials: P
authors.lastname: Costanzo
authors.forename: M
authors.initials: M
authors.lastname: De Rosa
authors.forename: T
authors.initials: T
authors.lastname: Russo
authors.forename: F
authors.initials: F
authors.lastname: Salvatore
authors.forename: P
authors.initials: P
authors.lastname: Izzo
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D016895:Culture Media, Serum-Free; D004247:DNA; D005091:Exons; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007438:Introns; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D015739:Nocodazole / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic
chemical_list: D016895:Culture Media, Serum-Free; D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D005634:Fructose-Bisphosphate Aldolase; D015739:Nocodazole