Direct detection of Vibrio cholerae in stool samples.
A direct method to detect Vibrio cholerae in stool samples was developed by using a PCR procedure that did not require a DNA purification step. Dilution (1/100) of stool samples prevented inhibition of the reaction by contaminants, and two consecutive PCRs, the second one with a nested primer, achieved the desired sensitivity. Comparison of the results obtained from stool swab samples processed by the two-step PCR and by an enzyme-linked immunosorbent assay using GM1 as the capture molecule showed that the former is more sensitive and gave positive results even when V. cholerae was not culturable or dead.
Varela
P
P
Instituto Nacional de Microbiología Carlos G Malbrán, Buenos Aires, Argentina.
Pollevick
G D
GD
Rivas
M
M
Chinen
I
I
Binsztein
N
N
Frasch
A C
AC
Ugalde
R A
RA
D001118:Argentina; D001483:Base Sequence; D002771:Cholera / Q000175:diagnosis* / Q000382:microbiology; D002772:Cholera Toxin / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification*
D017931:DNA Primers; D004269:DNA, Bacterial; D002772:Cholera Toxin