Effect of several electrolyte preservation solutions on the water permeability of isolated cortical collecting ducts perfused in vitro.
The extent of nephron injury after long-term preservation is unknown. An experimental protocol was designed to study the effects of 1 and 24 h of cold storage in preservation solution on renal slices of rabbit cortical collecting duct (CCD) isolated and perfused in vitro and evaluated by water hydraulic conductivity. The preservation solutions were Ringer-bicarbonate, Collins, Euro-Collins, and University of Wisconsin (UW). Under these experimental preservation conditions, the following results were observed: after 1 h of preservation, the CCDs obtained from slices incubated in Ringer-bicarbonate, Collins, and Euro-Collins solutions at 4 degrees C presented a high basal hydraulic conductivity compared to the control group and responded well to arginine-vasopressin (AVP) water permeability stimulation. The CCDs derived from renal slices stored for 24 h in Ringer-bicarbonate solution at 4 degrees C showed no function, while those preserved in Collins and Euro-Collins solutions showed a lack of responsiveness to AVP water permeability stimulation. The CCDs preserved in UW solution showed better values than those preserved in the other solutions, although there was a decrease in hydraulic conductivity after 24 h of preservation. These data show that long-term cold preservation with flushed solutions impairs the cortical collecting duct response to AVP.
Ribeiro
C A
CA
Department of Physiology and Biophysics, Faculty of Medical Sciences, State University of Campinas, São Paulo, Brazil.
Falkenstein
D
D
Figueiredo
J F
JF
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D004573:Electrolytes; D005069:Evaluation Studies as Topic; D005978:Glutathione; D006982:Hypertonic Solutions; D066298:In Vitro Techniques; D007328:Insulin; D007552:Isotonic Solutions; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010477:Perfusion; D010539:Permeability / Q000187:drug effects; D011817:Rabbits; D011887:Raffinose; D000077331:Ringer's Solution; D014867:Water / Q000378:metabolism
C024102:Collins' solution; D004573:Electrolytes; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D014867:Water; D001127:Arginine Vasopressin; D000493:Allopurinol; D000077331:Ringer's Solution; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose