Sequence of Rhodococcus gene cluster encoding the subunits of ethanolamine ammonia-lyase and an APC-like permease.
Sequence analysis of a 5173-bp genomic fragment from the nocardioform actinomycete Rhodococcus sp. strain NI86/21 revealed the presence of two genes, eutB and eutC, encoding the putative homologues of the large and small subunits of the ethanolamine ammonia-lyase, respectively, from Salmonella typhimurium. This is the first report of the characterization of these genes in a Gram-positive species. Immediately upstream of eutB, a gene encoding a putative permease of the APC (amino acids, polyamines, choline) transporter family was located. At present, no other Gram-positive members of this permease family are known. The translational coupling of these eut genes suggests an operon-like organization of the ethanolamine genes in Rhodococcus species. A truncated open reading frame downstream of eutC contained an N-terminal motif characteristic of membrane-anchored lipoproteins.
De Mot
R
R
F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium.
Nagy
I
I
Schoofs
G
G
Vanderleyden
J
J
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004981:Ethanolamine Ammonia-Lyase / Q000235:genetics*; D005798:Genes, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D010802:Phylogeny; D012240:Rhodococcus / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D004981:Ethanolamine Ammonia-Lyase