Thiopyronine- and 8-methoxypsoralen-sensitized photodynamic effect on cell growth, colony forming ability and RNA synthesis in Saccharomyces mutants deficient in DNA repair.
We compared the photodynamic effects of thiopyronine (TP) and visible light, and 8-methoxypsoralen (8-MOP) and ultraviolet A (UV-A) light, on growth, colony forming ability and RNA synthesis in a repair-proficient Saccharomyces strain and three mutants deficient in DNA repair mechanisms (DNA repair assays). With 8-MOP and UV-A repair-deficient mutants were significantly more sensitive than the repair-proficient strain indicating that the system is sensitive for the detection of DNA damage. With TP and visible light, the photodynamic effects were comparable in the mutants and the control, indicating no DNA damage. These results support previous work showing that the main target of TP photosensitization in eukaryotes is not nuclear DNA.
Roth
R M
RM
Biology Division, Illinois Institute of Technology, Chicago 60616.
Takamori
Y
Y
Lochmann
E R
ER
D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D008730:Methoxsalen / Q000494:pharmacology*; D010778:Photochemotherapy; D011754:Pyronine / Q000031:analogs & derivatives* / Q000494:pharmacology; D012331:RNA, Fungal / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000187:drug effects
D012331:RNA, Fungal; C005792:thiopyronine; D008730:Methoxsalen; D011754:Pyronine