Comparison of solution structure of free and complexed lac operator by molecular modelling with NMR constraints.
The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator adopt both a right-handed B helical conformation, but a more detailed analysis of the conformational parameters using the Curves program shows striking differences in the groove geometries, the rises, the twists and the total bending.
Gincel
E
E
CBM-CNRS, Orléans, France.
Lancelot
G
G
Maurizot
J C
JC
Thuong
N T
NT
Vovelle
F
F
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D007763:Lac Operon; D007786:Lactose Factors / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000737:chemistry*
D012097:Repressor Proteins