Glomerular metalloprotease activity in streptozotocin- treated rats and in spontaneously diabetic rats (BB/DP).
Differences in the type and extent of diabetic glomerular injury occur in rats made diabetic with streptozotocin (STZ) and in a spontaneously diabetic rat model of the BB/Wor strain. The diabetes prone (BB/DP) rat develops no mesangial matrix expansion with diabetes, whereas the STZ-treated Sprague Dawley rat does. The present study was performed to determine if glomerular metalloprotease activities were decreased in these two rat models of diabetes. Rats were studied at 8 weeks following the onset of hyperglycemia. All diabetic rats were maintained moderately hyperglycemic (plasma glucose = 350 mg/dL) with daily injection of insulin. Glomerular preparations were made and metalloprotease activities were assayed by hydrolysis of gelatin. Metalloprotease activities were decreased by 40-50% in STZ-treated Sprague Dawley rats, but not in BB/DP rats or diabetes resistant rats (BD/DR--the control for the BB/DP) made diabetic by STZ-injection. These data suggest that the decrease in glomerular metalloprotease activity may contribute to mesangial matrix expansion in the diabetic Sprague Dawley rats.
Reckelhoff
J F
JF
Department of Physiology, University of Mississippi Medical Center, Jackson 39216.
Tygart
V L
VL
Racusen
L C
LC
Dzielak
D J
DJ
D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000201:enzymology*; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy / Q000201:enzymology*; D004195:Disease Models, Animal; D007328:Insulin / Q000627:therapeutic use; D007668:Kidney / Q000473:pathology; D007678:Kidney Glomerulus / Q000201:enzymology*; D008297:Male; D008666:Metalloendopeptidases / Q000032:analysis*; D009929:Organ Size; D051381:Rats; D011913:Rats, Inbred BB; D017207:Rats, Sprague-Dawley
D007328:Insulin; D008666:Metalloendopeptidases