Efficient entrapment of amikacin and teicoplanin in liposomes.
A higher encapsulation rate was obtained using the dehydration-rehydration method compared with the reverse-phase evaporation technique in negative multilamellar vesicles with amikacin (AMK) (45% versus 15%; P < 0.05) and teicoplanin (TCP) (34% versus 25%; P < 0.05). The addition of 250 mM sucrose to AMK- or TCP-containing liposomes without prior drying prevented a significant decrease in antibiotic content in unilamellar and multilamellar vesicles over a 3-month period at -70 degrees C.
Ravaoarinoro
M
M
Hôtel-Dieu de Montréal, Qué., Canada.
Toma
E
E
Agbaba
O
O
Morisset
R
R
D000583:Amikacin / Q000008:administration & dosage* / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D002784:Cholesterol / Q000737:chemistry; D004337:Drug Carriers; D004355:Drug Stability; D008081:Liposomes; D008854:Microscopy, Electron; D010743:Phospholipids / Q000737:chemistry; D017334:Teicoplanin / Q000008:administration & dosage* / Q000737:chemistry
D004337:Drug Carriers; D008081:Liposomes; D010743:Phospholipids; D017334:Teicoplanin; D000583:Amikacin; D002784:Cholesterol