Inhibition of mammary gland carcinogenesis by green tea catechins and other naturally occurring antioxidants in female Sprague-Dawley rats pretreated with 7,12-dimethylbenz[alpha]anthracene.
Effects of the naturally occurring antioxidants on mammary gland carcinogenesis were examined in female Sprague-Dawley rats pretreated with 7,12-dimethylbenz[alpha]anthracene (DMBA). Groups of 15-16 7-week-old rats received a 50 mg/kg body weight intra-gastric dose of DMBA, and starting one week thereafter placed on diet containing 0.4% catechol, 1.0% gamma-oryzanol, 2.0% phytic acid, 1.0% green tea catechins (GTC), 1.0% tannic acid or basal diet alone for 35 weeks. Although the final incidences and multiplicities of mammary tumors were not significantly different between DMBA-treated groups, the numbers of survivors in the antioxidant-treated groups at the end of the experiment at week 36 were significantly higher than in the basal diet group. In particular, the survival rate of the GTC group at 93.8% strongly contrasted with that of only 33.3% for rats on the basal diet. At the end of week 18, when all the animals were still alive, the average size of palpable mammary tumors was significantly smaller in the catechol, phytic acid and catechins groups. These results indicate that antioxidants, and GTC in particular, inhibit rat mammary gland carcinogenesis after DMBA initiation.
Hirose
M
M
First Department of Pathology, Nagoya City University, Medical School, Japan.
Hoshiya
T
T
Akagi
K
K
Futakuchi
M
M
Ito
N
N
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000970:Antineoplastic Agents; D000975:Antioxidants / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology; D004428:Ear Neoplasms / Q000139:chemically induced; D005260:Female; D047348:Hydrolyzable Tannins / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced*; D009374:Neoplasms, Experimental / Q000139:chemically induced; D010666:Phenylpropionates / Q000494:pharmacology; D010833:Phytic Acid / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea
D000970:Antineoplastic Agents; D000975:Antioxidants; D002396:Catechols; D047348:Hydrolyzable Tannins; D010666:Phenylpropionates; D013662:Tea; D015127:9,10-Dimethyl-1,2-benzanthracene; D010833:Phytic Acid; C013172:gamma-oryzanol