title: Hydroxyl radical generation in skeletal muscle atrophied by immobilization.
abstract: Male Wistar rats (15 weeks old), the ankle joints of one hindlimb of which were immobilized in the extended position for 12 days, were injected with salicylate as a trapping agent for hydroxyl radicals before sacrifice. Atrophied and contralateral soleus, typical slow red muscles were collected and their levels of 2,3-dihydroxybenzoic acid (2,3-DHB), one of the main reaction products formed by the attack of hydroxyl radical on salicylate, were determined using high-performance liquid chromatography with an electrochemical detector. There was a significant increase in 2,3-DHB in the atrophied muscle. This result strongly suggests the enhanced generation in vivo of hydroxyl radicals in atrophied muscle.
authors.affiliation: Department of Hygiene, Faculty of Medicine, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kondo
authors.forename: K
authors.initials: K
authors.lastname: Nishino
authors.forename: Y
authors.initials: Y
authors.lastname: Itokawa
mesh_terms: D000818:Animals; D016166:Free Radical Scavengers; D062385:Hydroxybenzoates / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism*; D007103:Immobilization; D008297:Male; D009132:Muscles / Q000378:metabolism*; D009133:Muscular Atrophy / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012459:Salicylates; D020156:Salicylic Acid
chemical_list: D016166:Free Radical Scavengers; D062385:Hydroxybenzoates; D012459:Salicylates; D017665:Hydroxyl Radical; C009135:2,3-dihydroxybenzoic acid; D020156:Salicylic Acid