Protective effects of Nocardia delipidated cell mitogen on the mucosa of the small intestine after irradiation of germ-free piglets.
The radioprotective effect of the bacterial immunomodulator Nocardia delipidated cell mitogen (NDCM) on intestinal mucosa and disaccharidase activities was studied in irradiated germ-free piglets. Three-week-old germ-free (GF) piglets were intragastrically pretreated with 1 mg NDCM per 1 kg body weight. The piglets were whole-body irradiated with 2.5 Gray five days after the NDCM pretreatment and sacrificed eight days after irradiation. In the non-irradiated group of GF piglets, NDCM application stimulated lactase activity and markedly increased sucrase activity. This stimulatory effect of NDCM disappeared after irradiation and the piglets exhibited a normal activity of lactase in the jejunal brush-border membrane vesicles, while the sucrase activity decreased to the level found in irradiated controls. NDCM-pretreated intestinal mucosa contained some infrequent lymphocytes which disappeared from the control irradiated tissue. It also exhibited less injury of the epithelium and stroma cells.
Kozakova
H
H
Dept. Immunology and Gnotobiology, Academy of Sciences of the Czech Rep., Prague.
Mandel
L
L
Trebichavsky
I
I
Kolinska
J
J
Barot-Ciorbaru
R
R
D000818:Animals; D005720:Gamma Rays; D005856:Germ-Free Life; D007082:Ileum / Q000166:cytology; D007413:Intestinal Mucosa / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D007583:Jejunum / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D043322:Lactase; D008871:Microvilli / Q000528:radiation effects / Q000648:ultrastructure; D008934:Mitogens / Q000494:pharmacology*; D009615:Nocardia / Q000737:chemistry*; D009930:Organic Chemicals; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013393:Sucrase / Q000378:metabolism; D013552:Swine; D001616:beta-Galactosidase / Q000378:metabolism
D008934:Mitogens; C101327:Nocardia delipidated cell mitogen; D009930:Organic Chemicals; D011837:Radiation-Protective Agents; D043322:Lactase; D001616:beta-Galactosidase; D013393:Sucrase