title: The melting of native domain structure in effector activation of IgG studied by using congo red as a specific probe.
abstract: The nature of structural changes in IgG molecules associated with the binding to antigen and/or heat aggregation was studied using bis azo dye (Congo Red) as the specific probe. It was found, that protein conformation responsible for binding the dye represents an unfolding intermediate with properties corresponding to a molten globule state. The properties of the dye-protein complex reveal the signs of an unfolding of the peptide chain with simultaneously preserved relatively compact packing. Immunoglobulins which were induced by heating, or binding to antigen in order to form the complex with dye ligands, become more susceptible for digestion. The main peptide of molecular weight 30,000 D which appears in products was suggested to originate from a heavy chain after its splitting in the region of CH1 domain. The energetic evaluation of stability of IgG domains also indicates that CH1 is the least stable fragment of the heavy chain and its conformation may be destabilized first. It was concluded that destabilized tertiary packing of antibodies bound to antigen may favour the association of closely situated immunoglobulin molecules increasing the stability of the immune complex and influencing in the result its effector activity.
authors.affiliation: Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Cracow, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Piekarska
authors.forename: I
authors.initials: I
authors.lastname: Roterman
authors.forename: J
authors.initials: J
authors.lastname: Rybarska
authors.forename: L
authors.initials: L
authors.lastname: Koniczny
authors.forename: J
authors.initials: J
authors.lastname: Kaszuba
mesh_terms: D000936:Antigen-Antibody Complex / Q000737:chemistry*; D003224:Congo Red; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry*; D007202:Indicators and Reagents; D007700:Kinetics; D008970:Molecular Weight; D011487:Protein Conformation; D014508:Urea / Q000737:chemistry
chemical_list: D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D003224:Congo Red; D014508:Urea