Efficient identification and regional positioning of YAC and cosmid clones to human chromosome 21 by radiation fusion hybrids.
The use of integrated mapping strategies involving bacterial, yeast, and rodent cells as hosts simplifies the construction of maps, which combine long-range order, high resolution, and easy access to the cloned DNA. Radiation-fusion hybrids offer a specially powerful long-range mapping system for human chromosomes. We describe here techniques for establishing a radiation-fusion hybrid map of Chromosome (Chr) 21q and its integration with local information on YAC and cosmid positions.
Kumlien
J
J
Department of Genome Analysis, Imperial Cancer Research Fund, London, UK.
Labella
T
T
Zehetner
G
G
Vatcheva
R
R
Nizetic
D
D
Lehrach
H
H
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast; D002891:Chromosomes, Human, Pair 21; D006224:Cricetinae; D004926:Escherichia coli; D005819:Genetic Markers; D005821:Genetic Techniques; D005822:Genetic Vectors; D005838:Genotype; D006801:Humans; D006822:Hybrid Cells
D005819:Genetic Markers