title: In vitro phagocytosis of polylactide microspheres by retinal pigment epithelial cells and intracellular drug release.
abstract: We investigated phagocytosis of biodegradable microspheres containing a drug by retinal pigment epithelial (RPE) cells and drug release within the cells to evaluate the potential usefulness of microspheres for intracellular drug delivery. The biodegradable polymers used were L-lactic acid, and DL-lactic acid with different molecular weights or the copolymers of different monomer compositions. The microspheres containing a non-bioactive fluorescent dye (rhodamine 6GX) as a model drug, were prepared by a solvent evaporation method. The in vitro release of the dye from the microspheres was examined. Phagocytosis of the microspheres by RPE cells was conducted to evaluate the extent of phagocytosis by phase-contrast microscopy and transmission electron microscopy. The RPE cells ingesting the microspheres at different stages were examined by fluorescent microscopy to estimate the intracellular release of the dye. The dye was released with time from every microsphere and the release was controlled by changing the type of polymers constituting microspheres. The microspheres containing the dye were phagocytosed by RPE cells and the dye was released intracellularly with time. The present study indicates that the drug incorporated in the microspheres was delivered into RPE cells by way of phagocytosis and released within the cells. It is concluded that this microsphere system is a promising delivery form capable of drug targeting to RPE cells.
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: Y
authors.initials: Y
authors.lastname: Ogura
authors.forename: T
authors.initials: T
authors.lastname: Moritera
authors.forename: Y
authors.initials: Y
authors.lastname: Honda
authors.forename: Y
authors.initials: Y
authors.lastname: Tabata
authors.forename: Y
authors.initials: Y
authors.lastname: Ikada
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D002417:Cattle; D002478:Cells, Cultured; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D008863:Microspheres; D010587:Phagocytosis / Q000502:physiology*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D011091:Polyesters; D012235:Rhodamines / Q000008:administration & dosage*
chemical_list: D003692:Delayed-Action Preparations; D011091:Polyesters; D012235:Rhodamines; C033616:poly(lactide)