title: Chloramphenicol extraction from milk by using the diphasic dialysis method followed by liquid chromatographic determination.
abstract: An economical, fast, and simple method for the extraction and liquid chromatographic determination of chloramphenicol (CAP) in milk is described. CAP is extracted by using a recently developed membrane-based method named "diphasic dialysis." CAP is detected and quantitated in the organic solvent used in dialysis without additional cleanup steps by reversed-phase liquid chromatography and UV detection (270 nm). The determination limit of CAP in milk was about 5 micrograms/L, although as little as 1 microgram/L could be detected under optimal working conditions.
authors.affiliation: Universidad Complutense, Facultad de Veterinaria, Departamento Patología Animal I, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Bayo
authors.forename: M A
authors.initials: MA
authors.lastname: Moreno
authors.forename: J
authors.initials: J
authors.lastname: Prieta
authors.forename: S
authors.initials: S
authors.lastname: Díaz
authors.forename: G
authors.initials: G
authors.lastname: Suárez
authors.forename: L
authors.initials: L
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D002701:Chloramphenicol / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D003956:Dialysis / Q000379:methods*; D008892:Milk / Q000737:chemistry*
chemical_list: D002701:Chloramphenicol