title: Evaluation of a semi-automated 24-hour commercial system for identification of Enterobacteriaceae and other gram-negative bacteria.
abstract: A semi-automated commercial system (ID 32 E, bioMérieux) for 24-hour identification of Enterobacteriaceae and other gram-negative fermentative and nonfermentative bacteria encountered in diagnostic microbiology was evaluated. Overall, the system correctly identified 506 (91.5%) of the 553 strains tested, 94 (17.0%) strains requiring additional tests for complete identification. Six (1.1%) strains were misidentified and 33 (6.0%) strains were not identified. Eight (1.4%) strains were not present in the database and were misidentified or not identified. The system is a suitable alternative to existing systems for the identification of Enterobacteriaceae and other gram-negative bacteria frequently encountered in clinical samples.
authors.affiliation: Laboratoire de Microbiologie, Faculté de Médecine Lyon-Nord, France.
authors.forename: D
authors.initials: D
authors.lastname: Monnet
authors.forename: D
authors.initials: D
authors.lastname: Lafay
authors.forename: M
authors.initials: M
authors.lastname: Desmonceaux
authors.forename: J M
authors.initials: JM
authors.lastname: Boeufgras
authors.forename: F
authors.initials: F
authors.lastname: Allard
authors.forename: J
authors.initials: J
authors.lastname: Freney
mesh_terms: D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D012680:Sensitivity and Specificity