[Effect of osmolarity of the medium on the chemiluminescence of human neutrophils].
The effect of osmolality of the incubation medium on the luminol-amplified chemiluminescence (CL) of human neutrophils stimulated by N-formyl-Met-Len-Phe (FMLP), phorbol-12-myristate-13-acetate (PMA), calcium ionophore A-23187, thermoaggregated IgG (aggIgG) and opsonized zymosan has been studied. It has been shown that increase of osmolality from 320 up to 420 mosM decreased the A-23187--and opsonized zymosan-induced CL responses by 90%. Under the same conditions PMA-, FMLP- and aggIgG-induced CL responses were decreased by 40-60%. The decrease of the osmolality of incubation medium resulted in 2-3 fold decrease of the A-23187-, PMA- and FMLP-induced CL and in 60-80% increase of opsonized zymosan- and aggIgG-induced CL. It is assumed that osmolality-mediated alteration of cell volume is an important regulatory step in the process of neutrophils' activation.
Kuchkina
N V
NV
Orlov
S N
SN
Pokudin
N I
NI
Chuchalin
A G
AG
D000001:Calcimycin / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D007700:Kinetics; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects*; D009994:Osmolar Concentration; D010100:Oxygen / Q000097:blood*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D007074:Immunoglobulin G; D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen
Vliianie osmotichnosti sredy na khemiliuminestsentsiiu neĭtrofilov cheloveka.