Differential ontogenetic appearance and regulation of stimulatory G protein isoforms in rat cerebral cortex by thyroid hormone deficiency.
We have examined the effects of perinatal hypothyroidism on the levels of several G protein subunits in developing rat cerebral cortex. Thyroid deficiency significantly reduced the levels of the short form of G alpha s (G alpha s-s) by 70% and 83%, on P17 and P22, respectively, but had no effect on the long form of G alpha s (G alpha s-1), except that the G alpha s-1 levels were moderately increased on P22. Compared with age-matched controls, no significant differences were observed for G alpha i-1, G alpha i-2, G alpha o, G alpha q/11, G beta 1 and G beta 2 immunoreactivity in the cerebral cortex of the 22-day-old hypothyroid pups. These findings suggest that thyroid hormones may play an important role in controlling the pattern of expression of G alpha s isoforms during neonatal brain development and the reduced levels of G alpha s-s may contribute to some of the developmental abnormalities seen with perinatal hypothyroidism by altering signal transduction and intercellular communication.
Wong
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Section of Biochemical Psychiatry, Clarke Institute of Psychiatry, Toronto, Ontario, Canada.
Warsh
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Sibony
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Li
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D000375:Aging; D000818:Animals; D002540:Cerebral Cortex / Q000254:growth & development* / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000276:immunology; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism*; D015151:Immunoblotting; D007536:Isomerism; D008713:Methimazole; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D008713:Methimazole; D019204:GTP-Binding Proteins