Mechanism of macrophage recognition of SH-oxidized erythrocytes: recognition of glycophorin A on erythrocytes by a macrophage receptor for sialosaccharides.
Mouse erythrocytes treated with diamide, an SH-oxidizing agent, attach to mouse resident peritoneal macrophages in the absence of serum. The mechanism by which macrophages recognize the SH-oxidized erythrocytes was investigated. Although phosphatidylserine-liposomes inhibited the macrophage recognition, there was no detectable phosphatidylserine on the outer surface of diamide-treated erythrocytes. It is unlikely that phosphatidylserine, that has been proposed to be a determinant in the recognition of some pathologic erythrocytes by macrophages, is involved in the recognition of diamide-treated erythrocytes. Sialyl lactose and glycophorin A effectively inhibited the macrophage recognition, while lactose and neuraminidase-treated glycophorin A did not. Disialoganglioside GD1a, but not monosialoganglioside GM1, partially inhibited the recognition. Trypsinized erythrocytes, in which majority of glycophorin A glycopeptides were expected to be removed from the cell surface, poorly attached to macrophages after diamide treatment. Therefore, it is likely that an interaction between glycophorin A on diamide-treated erythrocytes and a macrophage receptor for sialosaccharides is involved in the recognition. Similar inhibition specificity was observed in the macrophage recognition of erythrocytes treated with periodate, an oxidant that induces disulfide-dependent erythrocyte changes causing macrophage recognition, and of erythrocytes treated with SH-blocking agents, N-ethylmaleimide and p-chloromercuribenzoic acid, that were also found to be susceptible to macrophage recognition in the absence of serum. These results suggest that the macrophages recognize sialosaccharide chains of glycophorin A molecules on SH-oxidized or SH-blocked erythrocytes through a receptor for sialosaccharides.
Beppu
M
M
Tokyo College of Pharmacy, Japan.
Takahashi
T
T
Hayashi
T
T
Kikugawa
K
K
D000818:Animals; D002240:Carbohydrate Sequence; D002448:Cell Adhesion / Q000187:drug effects; D003958:Diamide / Q000494:pharmacology*; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D005732:Gangliosides / Q000494:pharmacology; D006021:Glycophorins / Q000737:chemistry* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010587:Phagocytosis; D010718:Phosphatidylserines / Q000032:analysis; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*
D005732:Gangliosides; D006021:Glycophorins; D010718:Phosphatidylserines; D011956:Receptors, Cell Surface; C034993:ganglioside receptor; C025447:sialogangliosides; D003958:Diamide