Cell culture adaptation of astrovirus involves a deletion.
Astroviruses have been adapted to culture by serial blind passage in primary human embryo cells. All viruses thus adapted possess a 45-nucleotide deletion relative to fecal viruses or isolates made in CaCo-2 cells; this deletion may be responsible for the change in host cell range.
Willcocks
M M
MM
School of Biological Sciences, University of Surrey, Guildford, United Kingdom.
Ashton
N
N
Kurtz
J B
JB
Cubitt
W D
WD
Carter
M J
MJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D001257:Mamastrovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014779:Virus Replication
D017931:DNA Primers; D014764:Viral Proteins