title: Elongation factor EF-1 delta, a new target for maturation-promoting factor in Xenopus oocytes.
abstract: A new physiological target for Cdc2 protein kinase has been identified. It corresponds to a protein EF-1 delta, a constituent of the nucleotide exchange factor EF-1 beta gamma delta, involved in the elongation step of protein synthesis. EF-1 delta is phosphorylated by Cdc2 kinase on threonine and serine residues. Threonine has been identified as Thr122 in the sequence VQVTPAAK. During oocyte maturation, Thr122 is phosphorylated at metaphase, when p34cdc2 is active. Phosphorylation studies revealed the presence of two post-translational regulated forms of EF-1 delta protein. Identification of two isoforms of the delta protein, together with the presence of two guanine-nucleotide exchange proteins (beta and delta) and physiologically regulated phosphorylation sites by Cdc2 kinase on gamma and delta proteins, implicate that EF-1 beta gamma delta exists in the cell under a multitude of macromolecular forms which suggests that EF-1 beta gamma delta is a sophisticated regulatory factor rather than a "housekeeping" element of the cell.
authors.affiliation: Laboratoire de Physiologie de la Reproduction, Université Pierre et Marie Curie, CNRS URA 1449, Institut National de la Recherche Agronómique, Paris, France.
authors.forename: O
authors.initials: O
authors.lastname: Mulner-Lorillon
authors.forename: O
authors.initials: O
authors.lastname: Minella
authors.forename: P
authors.initials: P
authors.lastname: Cormier
authors.forename: J P
authors.initials: JP
authors.lastname: Capony
authors.forename: J C
authors.initials: JC
authors.lastname: Cavadore
authors.forename: J
authors.initials: J
authors.lastname: Morales
authors.forename: R
authors.initials: R
authors.lastname: Poulhe
authors.forename: R
authors.initials: R
authors.lastname: Bellé
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D016203:CDC2 Protein Kinase / Q000378:metabolism; D016200:Maturation-Promoting Factor / Q000378:metabolism*; D008677:Metaphase; D008969:Molecular Sequence Data; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D009866:Oogenesis; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000378:metabolism*; D010766:Phosphorylation; D013379:Substrate Specificity; D014982:Xenopus laevis
chemical_list: D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D016203:CDC2 Protein Kinase; D016200:Maturation-Promoting Factor