title: Development of an enzyme-mediated assay for phenylalanine in blood spots.
abstract: We describe the optimization of a rapid procedure for the elution of phenylalanine from blood spots and the estimation of the amino acid eluted using an enzyme-mediated assay linked to a colorimetric detection system. The method is rapid, fully quantitative, interference-free, accurate and precise unlike many of the methods currently employed for phenylalanine determination. The performance of the proposed method may necessitate the review of reference ranges and cut-off assignment strategies.
authors.affiliation: Department of Clinical Biochemistry, London Hospital Medical College, UK.
authors.forename: R S
authors.initials: RS
authors.lastname: Campbell
authors.forename: R D
authors.initials: RD
authors.lastname: Hollifield
authors.forename: H
authors.initials: H
authors.lastname: Varsani
authors.forename: T P
authors.initials: TP
authors.lastname: Milligan
authors.forename: G
authors.initials: G
authors.lastname: Brearley
authors.forename: C P
authors.initials: CP
authors.lastname: Price
mesh_terms: D000328:Adult; D000594:Amino Acid Oxidoreductases; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D006801:Humans; D010649:Phenylalanine / Q000097:blood*; D012016:Reference Values; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D010649:Phenylalanine; D000594:Amino Acid Oxidoreductases; C022342:phenylalanine oxidase