Use of biodegradable mesh as a transport for a cultured uroepithelial graft: an improved method using collagen gel.
OBJECTIVE
To create an improved delivery vehicle for cultured uroepithelial cells using biodegradable mesh and collagen gel.


METHODS
Twenty uroepithelial grafts were prepared by seeding cultured urothelial cells onto previously prepared collagen gel-polyglactin mesh. As a control, cells were seeded onto 20 plain polyglactin mesh (without gel) squares in the same fashion.


RESULTS
The presence of urothelial cells was confirmed in all cultures by immunohistochemistry testing with anticytokeratin antibody. After 5 to 7 days, a confluent monolayer of cells covered 90% to 100% of the surface of all 20 collagen gel-mesh grafts. A confluent monolayer of cells did not form on any control graft (plain mesh). After 3 weeks, the cells had formed a sporadic, multiple cell layer confluence over 10% to 50% of the control graft surface; however, the mesh became friable and fell apart.


CONCLUSIONS
By combining collagen gel with a biodegradable mesh scaffold, we created a surgically implantable cultured uroepithelial graft. The production of the collagen gel-mesh vehicle is simple and inexpensive. With this technique, the use of cultured urothelium for reconstructive urologic surgery can be investigated in greater detail.
Hakim
S
S
Department of Surgery, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire.
Merguerian
P A
PA
Chavez
D R
DR
D000818:Animals; D001673:Biodegradation, Environmental; D002478:Cells, Cultured; D003094:Collagen; D046508:Culture Techniques; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology / Q000637:transplantation; D005782:Gels; D011098:Polyglactin 910; D011817:Rabbits; D051381:Rats; D013526:Surgical Mesh; D001743:Urinary Bladder / Q000166:cytology / Q000502:physiology / Q000637:transplantation*
D005782:Gels; D011098:Polyglactin 910; D003094:Collagen