title: XDJ1, a gene encoding a novel non-essential DnaJ homologue from Saccharomyces cerevisiae.
abstract: The gene encoding a novel DnaJ-like protein, termed Xdj1, has been identified by amplification of Saccharomyces cerevisiae genomic DNA. An open reading frame of 1380 bp was detected. Disruption of XDJ1 did not yield any detectable new phenotype. A double-deletion strain containing a disruption of both XDJ1 and YDJ1, another gene coding for a DnaJ-like protein, was still viable. Under a variety of growth conditions, no XDJ1 transcripts could be detected by Northern blot analysis and no translation product was found by immunoblotting with antibody against Xdj1 produced in Escherichia coli. Thus, XDJ1 is either expressed only under very specific conditions or represents a silent gene.
authors.affiliation: Institut für Physiologische Chemie, München, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Schwarz
authors.forename: B
authors.initials: B
authors.lastname: Westermann
authors.forename: A J
authors.initials: AJ
authors.lastname: Caplan
authors.forename: G
authors.initials: G
authors.lastname: Ludwig
authors.forename: W
authors.initials: W
authors.lastname: Neupert
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D004271:DNA, Fungal; C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; C088620:XDJ1 protein, S cerevisiae