title: [Amplification of DNA for 15-30 minutes in single-use pipette tips].
abstract: Amplification of 200-1000-bp DNA fragments was performed in 15-30 min using a rapid thermal cycler based on the commercial instrument TC-1000-1 (IRLEN, St. Petersburg, Russia). Plastic pipette tips were used as thin walled, high surface to volume-ratio tubes, to increase the rate of heating (cooling) of 20 microliters samples, which allowed the time required for DNA amplification to be considerably reduced (5-10 times).
authors.forename: A N
authors.initials: AN
authors.lastname: Tret'iakov
authors.forename: V M
authors.initials: VM
authors.lastname: Gel'fand
authors.forename: R A
authors.initials: RA
authors.lastname: Pantina
authors.forename: S P
authors.initials: SP
authors.lastname: Shevtsov
authors.forename: S A
authors.initials: SA
authors.lastname: Bulat
mesh_terms: D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D012568:Schizosaccharomyces / Q000235:genetics
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D004247:DNA
vernacular_title: Amplifikatsiia DNK za 15-30 minut v odnorazovykh nakonechnikakh dlia mikrodozatorov.