Determination of sertraline and desmethylsertraline in human serum using copolymeric bonded-phase extraction, liquid chromatography and gas chromatography-mass spectrometry.
The determination of the new antidepressant drug sertraline and its main metabolite, desmethylsertraline, in human serum is described. A new solid-phase extraction method employing the dual functionality Clean Screen cartridge is presented followed by reversed-phase liquid chromatographic (LC) analysis. The sample preparation yielded extremely clean extracts and absolute recoveries in excess of 90% for both drugs from human serum. The response of the LC system was linear over the concentration range 0.01-2.5 mg/l for both sertraline and desmethylsertraline with a limit of detection of 0.01 mg/l. A gas chromatographic-mass spectrometric (GC-MS) system is also described should confirmation of the drugs be necessary.
Rogowsky
D
D
United Chemical Technologies, Bristol, PA 19007.
Marr
M
M
Long
G
G
Moore
C
C
D015057:1-Naphthylamine / Q000031:analogs & derivatives* / Q000032:analysis; D000928:Antidepressive Agents / Q000097:blood*; D002853:Chromatography, Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D020280:Sertraline / Q000031:analogs & derivatives; D013056:Spectrophotometry, Ultraviolet
D000928:Antidepressive Agents; D015057:1-Naphthylamine; C065162:desmethylsertraline; D020280:Sertraline