title: Another putative heat-shock gene and aminoacyl-tRNA synthetase gene are located upstream from the grpE-like and dnaK-like genes in Chlamydia trachomatis.
abstract: The 4.1-kb sequence of genomic DNA located upstream from the Chlamydia trachomatis grpE-like and dnaK-like heat shock (HS) genes was determined. Another putative HS gene was located just 5' to grpE along with an inverted repeat (IR) sequence proposed to be involved in HS regulation. The overall organization of this locus in Chlamydia resembles that of Bacillus subtilis, rather than Escherichia coli. Two other open reading frames (ORFs) were found in the sequence, one of which has homology to aminoacyl-tRNA synthetases. The other ORF has no significant homology to reported genes. We also examined the codon usage bias for these newly identified chlamydial ORFs and for previously reported chlamydial genes, and found them to be different from E. coli.
authors.affiliation: Department of Microbiology and Immunology, University of North Carolina, School of Medicine, Chapel Hill 27599-7290.
authors.forename: D H
authors.initials: DH
authors.lastname: Schmiel
authors.forename: P B
authors.initials: PB
authors.lastname: Wyrick
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000235:genetics*; D002874:Chromosome Mapping; D003062:Codon; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C068113:GrpE protein, Bacteria; C489400:GrpE protein, E coli; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C063842:dnaK protein, E coli; D000604:Amino Acyl-tRNA Synthetases