Unaltered aconitase activity, but decreased complex I activity in substantia nigra pars compacta of patients with Parkinson's disease.
Substantia nigra pars compacta of seven patients who had died of Parkinson's disease, has been investigated for the iron-depending aconitase (reactions I and II). In addition we analysed respiratory chain enzymes. While complex I activity of the respiratory chain was significantly reduced, other enzymes of this pathway were unaltered. The citric acid cycle enzyme aconitase (reactions I and II) showed no difference between patients and controls. Thus this ferrous iron dependent and oxidatively sensitive enzyme is not affected by the unphysiological high amount of ferric iron and the 'oxidative stress' present in substantia nigra of parkinsonian patients.
Janetzky
B
B
Department of Neurology, University of Würzburg, Germany.
Hauck
S
S
Youdim
M B
MB
Riederer
P
P
Jellinger
K
K
Pantucek
F
F
Zöchling
R
R
Boissl
K W
KW
Reichmann
H
H
D000154:Aconitate Hydratase / Q000378:metabolism*; D000368:Aged; D002540:Cerebral Cortex / Q000201:enzymology; D002950:Citrate (si)-Synthase / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000378:metabolism; D008875:Middle Aged; D009245:NADH Dehydrogenase / Q000378:metabolism*; D010300:Parkinson Disease / Q000201:enzymology*; D011699:Putamen / Q000201:enzymology; D013378:Substantia Nigra / Q000201:enzymology*
D007501:Iron; D009245:NADH Dehydrogenase; D002950:Citrate (si)-Synthase; D000154:Aconitate Hydratase