Role of a distal promoter element in the S-phase control of the human H1.2 histone gene transcription.
The expression of one of the human main type H1 histone genes (termed H1.2) appears to be regulated by several trans-acting factors. Upstream of consensus regulatory regions, such as the TATA-, CCAAT- and H1-box (AAACACA) sequences, a crucial control site is located between nucleotide positions -536 and -412 (relative to the ATG initiation site). Removal of this promoter portion causes in chloramphenicol acetyl transferase reporter gene constructs a loss of the S-phase control function of the H1.2 promoter in HeLa cells. Electrophoretic mobility-shift assay and DNase I footprinting analysis suggest that the H1-box variant AAACAGA is a potential control element within the distal promoter region.
Eilers
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Abt. Molekularbiologie, Georg-August-Universität Göttingen, Germany.
Bouterfa
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Triebe
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Doenecke
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D
D016590:Aphidicolin / Q000494:pharmacology; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003850:Deoxyribonuclease I; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006657:Histones / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016196:S Phase; D016385:TATA Box; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection
D006657:Histones; D009841:Oligonucleotides; D016590:Aphidicolin; D015500:Chloramphenicol O-Acetyltransferase; D003850:Deoxyribonuclease I