title: [Cloning and sequencing of a DNA fragment from Plasmodium falciparum FCC1/HN isolate].
abstract: A specific DNA fragment isolated from Plasmodium falciparum FCC1/HN isolate has been cloned in Bam H1 site of pUC18 and first partially sequenced by Sanger's method. The results show: G+C percent of DNA sequence is 26.8%, and the cloned DNA has many restriction sites which are conventional for subcloning. The authors suggest that this clone and sequence may be used as a guide for developing a DNA probe or PCR primers.
authors.affiliation: Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, Shanghai.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: J W
authors.initials: JW
authors.lastname: He
authors.forename: Z X
authors.initials: ZX
authors.lastname: Zheng
authors.forename: W B
authors.initials: WB
authors.lastname: Guan
authors.forename: Y C
authors.initials: YC
authors.lastname: Zhou
authors.forename: L R
authors.initials: LR
authors.lastname: Cai
authors.forename: F Y
authors.initials: FY
authors.lastname: Ye
authors.forename: H
authors.initials: H
authors.lastname: Xin
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010963:Plasmodium falciparum / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D010446:Peptide Fragments