[In vitro separation of bone marrow nuclear cells with methylcellulose and its application to autologous bone marrow transplantation in patients with leukemia].
We studied bone marrow separated with 0.05% methylcellulose and cryopreservatized with liquid nitrogen. The separation time was 43 minutes. The collection rates of nuclear cells and CFU-GM were 79.0 +/- 5.10% and 93.0 +/- 3.10% in normal marrow samples and 83.4 +/- 15.45% and 91.0 +/- 8.32% in those of acute leukemia patients. The rate of residual erythocytes was 12.5% and the bone marrow volume could be reduced by 55.2%. Application of this technique to autologous bone marrow transplantation in four patients with acute leukemia reconstituted all of their hemopoietic functions and no toxic side effect was found after transplantation.
Bai
H
H
Department of Hematology, Lanzhou General Hospital, Gansu.
Da
W M
WM
Dong
G P
GP
D001853:Bone Marrow / Q000473:pathology*; D016026:Bone Marrow Transplantation; D002469:Cell Separation / Q000379:methods; D015925:Cryopreservation; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000628:therapy*; D008747:Methylcellulose; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy*; D014182:Transplantation, Autologous
D008747:Methylcellulose