title: Presence of cytomegalovirus genome and leucocyte subsets in renal transplant biopsies. Relationship with prognosis.
abstract: The influence of immunosuppressant therapy and of the presence of CMV genome on the distribution of lymphoid subpopulations of the inflammatory infiltrate in renal graft rejection was analyzed, as was the role of both factors in the evolution and survival of the graft. The study included 22 patients treated with Cyclosporin A (CsA) and 22 patients treated with Azathioprine (AZA). Inflammatory infiltrate was studied by immunostaining with a panel of monoclonal antibodies, and CMV DNA was detected by in situ hybridization on tissue sections. In patients treated with CsA, increased cellularity was found at both glomerular and interstitial levels, consisting mainly of macrophages and T-cells, which was consistent with the higher rate of glomerulointerstitial rejection found in this group. In contrast, the vascular type of rejection predominated in AZA treated patients. However, the presence of CMV DNA did not influence the phenotype of the inflammatory infiltrate, and was not associated with any specific lesion. Furthermore, the final outcome of the renal graft was independent of the detection of CMV. Therefore, this study provides no evidence of any active role of the CMV genome in renal graft rejection, and suggests that therapy should be adapted to the type of rejection as defined on morphologic and immunophenotypic grounds.
authors.affiliation: Department of Pathology, University Hospital, School of Medicine, University of Granada, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Lardelli
authors.forename: D
authors.initials: D
authors.lastname: Aguilar
authors.forename: M
authors.initials: M
authors.lastname: Gómez-Morales
authors.forename: I
authors.initials: I
authors.lastname: Antón
authors.forename: N
authors.initials: N
authors.lastname: Navarro
authors.forename: A
authors.initials: A
authors.lastname: Montes
authors.forename: M
authors.initials: M
authors.lastname: Andújar
authors.forename: M
authors.initials: M
authors.lastname: Bustos
authors.forename: F
authors.initials: F
authors.lastname: O'Valle
authors.forename: J
authors.initials: J
authors.lastname: Aneiros
mesh_terms: D000328:Adult; D001379:Azathioprine / Q000627:therapeutic use; D016572:Cyclosporine / Q000627:therapeutic use; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D016679:Genome, Viral; D006084:Graft Rejection; D006801:Humans; D007150:Immunohistochemistry; D007165:Immunosuppression Therapy; D017403:In Situ Hybridization; D007668:Kidney / Q000382:microbiology* / Q000473:pathology*; D016030:Kidney Transplantation; D007962:Leukocytes / Q000145:classification*; D011379:Prognosis
chemical_list: D004279:DNA, Viral; D016572:Cyclosporine; D001379:Azathioprine