An improved technique for the 2H/1H analysis of urines from diabetic volunteers.
The H2-H2O ambient-temperature equilibration technique for the determination of 2H/1H ratios in urinary waters from diabetic subjects provides improved accuracy over the conventional Zn reduction technique. The standard deviation, approximately 1-2%, is at least a factor of three better than that of the Zn reduction technique on urinary waters from diabetic volunteers. Experiments with pure water and solutions containing glucose, urea and albumen indicate that there is no measurable bias in the hydrogen equilibration technique.
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Clinical Diabetes and Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Phoenix, Arizona 85016.
Harper
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D001786:Blood Glucose / Q000378:metabolism; D003903:Deuterium / Q000378:metabolism; D003920:Diabetes Mellitus / Q000652:urine*; D005951:Glucose Tolerance Test; D006801:Humans; D007231:Infant, Newborn; D010084:Oxidation-Reduction; D014556:Urine / Q000737:chemistry; D015032:Zinc / Q000652:urine
D001786:Blood Glucose; D003903:Deuterium; D015032:Zinc