Glucosylation of chimeric proteins in the cell wall of Saccharomyces cerevisiae.
Extension of a reporter protein with the carboxyterminal thirty amino acids of the cell wall mannoprotein alpha-agglutinin of Saccharomyces cerevisiae resulted in incorporation of the chimeric protein in the cell wall. By Western analysis it was shown that the incorporated protein contained beta-1,6-glucan similar to endogenous cell wall proteins, whereas excreted reporter protein was not glucosylated. This suggests that beta-1,6-glucan is involved in anchoring mannoproteins in the cell wall.
van Berkel
M A
MA
Institute for Molecular Cell Biology, University of Amsterdam, BioCentrum Amsterdam, The Netherlands.
Caro
L H
LH
Montijn
R C
RC
Klis
F M
FM
D002473:Cell Wall / Q000378:metabolism*; D017930:Genes, Reporter; D005936:Glucans / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D000072235:Mating Factor; D008562:Membrane Glycoproteins / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D047071:beta-Glucans
D005936:Glucans; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D010455:Peptides; D047071:beta-Glucans; C025363:mannoproteins; C064197:beta-1,6-glucan; D000072235:Mating Factor; D005947:Glucose