title: Histopathologic effects of mitomycin C after trabeculectomy in human glaucomatous eyes with persistent hypotony.
abstract: We evaluated the histopathologic findings in seven patients who underwent surgical revision of the filtration site after trabeculectomy with mitomycin C because of persistent hypotonous maculopathy. Light microscopic examination of subconjunctival tissue and sclera demonstrated hypocellularity of fibroblasts and disruption of the normal architecture. Tissue fragments at the margin of the bleb wall demonstrated scarring and contained multiple fibroblasts. Additionally, we investigated the histopathologic changes in an eye obtained from a patient who died one week after a trabeculectomy with mitomycin C. Transmission electron microscopy showed myelin figures, increased melanolipofuscin granules, vacuolated cytoplasm, and disrupted mitochondria of the ciliary body epithelium underlying the site of mitomycin C application. On the basis of these findings, both overfiltration because of tissue disorganization of the filtering bleb and aqueous hyposecretion because of ciliary body toxicity might be involved in the causes of persistent hypotony after mitomycin C trabeculectomy.
authors.affiliation: Department of Ophthalmology, Academic Medical Center of the University of Amsterdam, The Netherlands.
authors.forename: R M
authors.initials: RM
authors.lastname: Nuyts
authors.forename: P C
authors.initials: PC
authors.lastname: Felten
authors.forename: E
authors.initials: E
authors.lastname: Pels
authors.forename: C T
authors.initials: CT
authors.lastname: Langerhorst
authors.forename: H C
authors.initials: HC
authors.lastname: Geijssen
authors.forename: H E
authors.initials: HE
authors.lastname: Grossniklaus
authors.forename: E L
authors.initials: EL
authors.lastname: Greve
mesh_terms: D000328:Adult; D000368:Aged; D002924:Ciliary Body / Q000187:drug effects / Q000648:ultrastructure; D003131:Combined Modality Therapy; D003228:Conjunctiva / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage; D005901:Glaucoma / Q000473:pathology* / Q000628:therapy; D006801:Humans; D008297:Male; D008875:Middle Aged; D016685:Mitomycin / Q000494:pharmacology*; D015814:Ocular Hypotension / Q000209:etiology / Q000473:pathology* / Q000628:therapy; D012086:Reoperation; D012590:Sclera / Q000187:drug effects / Q000648:ultrastructure; D013524:Surgical Flaps / Q000473:pathology; D013536:Suture Techniques / Q000009:adverse effects; D014130:Trabeculectomy
chemical_list: D016685:Mitomycin; D005472:Fluorouracil