[The use of streptococcal immunoglobulin Fc receptors in the technology of producing a pseudotuberculosis diagnostic test system].
The conditions of obtaining the IgM from hyperimmune sera to Yersinia pseudotuberculosis have been established. The process includes the adsorption of these sera by streptococci belonging to serological groups G and A and having receptors which bind the Fc-fragment of IgG and IgA molecules. The data confirming the fact that specific antibodies to Y. pseudotuberculosis belong to IgM have been obtained. The use of isolated IgM fractions for the preparation of a pseudotuberculosis diagnostic assay system has made it possible to establish that the new method of obtaining sensitin is economical and ensures a 3.5-fold greater yield of the conjugate and simplifies the technological cycle of its preparation.
Burova
L A
LA
Tseneva
G Ia
GIa
Kuliashova
L B
LB
Murashov
S V
SV
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000918:Antibody Specificity; D007114:Immunization; D007136:Immunoglobulins / Q000276:immunology* / Q000302:isolation & purification; D007159:Immunologic Tests / Q000379:methods; D011817:Rabbits; D011961:Receptors, Fc / Q000276:immunology* / Q000302:isolation & purification; D013291:Streptococcus / Q000276:immunology*; D015011:Yersinia pseudotuberculosis / Q000276:immunology; D015012:Yersinia pseudotuberculosis Infections / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D007136:Immunoglobulins; D011961:Receptors, Fc
Ispol'zovanie streptokokkovykh immunoglobulinovykh Fc-retseptorov v tekhnologii polucheniia diagnosticheskoĭ psevdotuberkuleznoĭ test-sistemy.