title: Different reinnervation patterns in the celiac/mesenteric and superior cervical ganglia following guanethidine sympathectomy in adult rats.
abstract: We sought to determine whether chronic guanethidine (Gu) treatment in adult rats produces depletion of sympathetic neurons and hyperinnervation by sensory neuropeptides in the celiac/superior mesenteric (C/SMG) ganglion. Rats received Gu 40 mg/kg per day i.p or saline for 5 weeks. Upon completion of treatment, the C/SMG and the superior cervical ganglion (SCG) were examined for neuropeptide Y (NPY), substance P (SP), calcitonin gene-related peptide (CGRP) and vasoactive intestinal polypeptide (VIP), both by immunocytochemistry (ICC) and radioimmunoassay (RIA). Gu produced marked depletion of NPY-containing neurons and NPY content in the C/SMG, similar to that in the SCG (-89 +/- 2 vs. -92 +/- 4%, respectively). SP and CGRP immunoreactivities were significantly higher in control C/SMG as compared with SCG; after Gu treatment, there was no significant increase in either SP or CGRP in the C/SMG, however, both increased in the SCG. In contrast, VIP levels were similar in the SCG and C/SMG in controls and increased in the C/SMG but not in the SCG after Gu treatment. Thus, in adult rats, the C/SMG is as susceptible as the SCG to Gu treatment; the different pattern of hyperinnervation by SP, CGRP and VIP of the C/SMG as compared with the SCG may reflect the different sources for these neuropeptides in prevertebral as compared with paravertebral ganglia.
authors.affiliation: Department of Neurology, Mayo Clinic, Rochester, MN 55905.
authors.forename: E E
authors.initials: EE
authors.lastname: Benarroch
authors.forename: P J
authors.initials: PJ
authors.lastname: Zollman
authors.forename: I L
authors.initials: IL
authors.lastname: Smithson
authors.forename: J D
authors.initials: JD
authors.lastname: Schmelzer
authors.forename: P A
authors.initials: PA
authors.lastname: Low
mesh_terms: D000818:Animals; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000502:physiology*; D006145:Guanethidine; D007150:Immunohistochemistry; D008297:Male; D009479:Neuropeptides / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D017783:Superior Cervical Ganglion / Q000166:cytology / Q000502:physiology*; D013563:Sympathectomy, Chemical
chemical_list: D009479:Neuropeptides; D006145:Guanethidine