title: Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa.
abstract: Human papillomavirus (HPV) infections in oral carcinomas and normal oral mucosa were studied by consensus primer screening and typing for HPV types 6/11, 16 and 18 DNA. After polymerase chain reaction (PCR) the DNA species of interest were identified by Southern blot hybridization with digoxigenin-labeled oligonucleotide probes. Frozen tissue and scrapings were equally suitable for HPV testing and yielded high HPV detection rates in carcinomas. By comparison, HPV analysis of paraffin-embedded material was much less efficient. HPV were demonstrated in 61.5% (16/26) of oral squamous cell carcinomas, high risk HPV 16 and 18 being the preferential types. The frequency of HPV detection in non-neoplastic mucosa of tumor patients decreased clearly with increasing distance from the tumor (range 26.9-3.8%) suggesting focal HPV infections. In contrast, normal buccal mucosa of a group of healthy volunteers contained HPV DNA only in 1% (1/97).
authors.affiliation: Institute of Pathology, University of Rostock, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Ostwald
authors.forename: P
authors.initials: P
authors.lastname: Müller
authors.forename: M
authors.initials: M
authors.lastname: Barten
authors.forename: K
authors.initials: K
authors.lastname: Rutsatz
authors.forename: M
authors.initials: M
authors.lastname: Sonnenburg
authors.forename: K
authors.initials: K
authors.lastname: Milde-Langosch
authors.forename: T
authors.initials: T
authors.lastname: Löning
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000473:pathology / Q000821:virology*; D015345:Oligonucleotide Probes; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral; D015345:Oligonucleotide Probes