[Interrelationship between Epstein-Barr virus and Sjögren's syndrome].
We designed a specific primer of EB virus Bam W fragment and EBV DNA sequence by using the polymerase chain reaction (PCR) to amplify the EBV DNA sequences and then performing the hybridization analysis (dot blot and Southern transfer) with a gamma 32P end-labelled internal oligonucleotide probe. There was a significantly higher positive rate of 24% (7/29) for the labial glands of Sjögren's syndrome patients than 68% (20/29) for gamma 32P Bam W probe. In situ DNA hybridization, with (alpha 35S) DCTP labelled EBV DNA probe, the EBV genomes were demonstrated in the labial glands of the Sjögren's syndrome patients (16/28). Moreover, the elevated content of EBV DNA was identified in those with more severe destruction of labial glands. We conclude that EBV has a normal site of latency at salivary glands in a nonpathogenic state, and may be reactivated in Sjögren's syndrome patients and play a role in the pathogenesis of this disease. The great sensitivity of PCR and the ability to analyze very small tissue biopsies (fresh or paraffin-embedded) make this technique applicable to clinical diagnosis.
He
L Y
LY
People's Hospital, Beijing Medical University.
Wang
J H
JH
Wang
S W
SW
D000328:Adult; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006566:Herpesviridae Infections; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012470:Salivary Glands, Minor / Q000382:microbiology; D012859:Sjogren's Syndrome / Q000382:microbiology*; D014412:Tumor Virus Infections
D004279:DNA, Viral