The complete nucleotide sequence and construction of an infectious cDNA clone of a highly virulent encephalomyocarditis virus.
The nucleotide sequence of a highly variant of the encephalomyocarditis virus, PV21, was determined. Discounting the poly(A) tail the viral genome is 7.861 kb in length, including a poly(C) region of 157 or 158 nucleotides. The sequence differs in 38 positions from another virulent EMCV variant, EMCV-R (1). However, the PV21 sequence shows only 85% identity with two nonlethal variants isolated in this laboratory (2). A cDNA clone covering the complete virus sequence including a poly(C) region of approximately 150 nucleotides was constructed. The recombinant virus was shown to be infectious in NMRI mice.
Zimmermann
A
A
Institut für Virologie der Universität zu Köln, Federal Republic of Germany.
Nelsen-Salz
B
B
Kruppenbacher
J P
JP
Eggers
H J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000302:isolation & purification*; D004686:Encephalomyocarditis virus / Q000235:genetics* / Q000472:pathogenicity; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D011817:Rabbits; D014774:Virulence
D018076:DNA, Complementary