title: Characterisation of angiotensin II binding to glomeruli from rainbow trout Oncorhynchus mykiss adapted to fresh water and seawater.
abstract: Specific binding of 125I-[Asn1, Val5]-angiotensin II to isolated glomeruli of freshwater-adapted and seawater-adapted rainbow trout, Oncorhynchus mykiss, reached a plateau after 20 min at 10 degrees and was displaced by nonradiolabelled [Asn1, Val5]-angiotensin II. Seawater adaptation increased the rate of dissociation of 125I-[Asn1, Val5]-angiotensin II from putative receptors. Competitive binding experiments revealed a single population of high-affinity receptors with a KD 0.3-0.4 x 10(-10) M, which was unchanged by seawater adaptation. Seawater adaptation did, however, reduce the density (Bmax) of the binding sites, which may reflect down-regulation by the higher plasma concentration of angiotensin II in seawater-adapted trout.
authors.affiliation: Department of Biological Sciences, Hatherly Laboratories, University of Exeter, United Kingdom.
authors.forename: C S
authors.initials: CS
authors.lastname: Cobb
authors.forename: J A
authors.initials: JA
authors.lastname: Brown
mesh_terms: D000222:Adaptation, Physiological; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D005260:Female; D005618:Fresh Water; D007457:Iodine Radioisotopes; D007678:Kidney Glomerulus / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D012623:Seawater
chemical_list: D007457:Iodine Radioisotopes; D000804:Angiotensin II