title: Angioimmunoblastic lymphadenopathy type of T-cell lymphoma and angioimmunoblastic lymphadenopathy: a clinicopathological and molecular biological study of 13 Chinese patients using polymerase chain reaction and paraffin-embedded tissues.
abstract: The morphological classification of angioimmunoblastic lymphadenopathy (AILD) or T-cell lymphoma of AILD-type (AILD-TCL) is still a subject of considerable difficulty and controversy. The aim of the current study was to examine the value of clinical, morphological, immunohistochemical variables in paraffin-embedded tissues in predicting the clonality of the respective lesion. Fifteen lymph node biopsies derived from 13 patients from Chengdu, China, were diagnosed as AILD or AILD-TCL and included in this study. The specimens were examined using a panel of monoclonal antibodies and a scoring system of morphological features. Clonality of the paraffin-embedded material was investigated using a novel polymerase chain reaction-technique to amplify rearranged T-cell receptor (TCR)-gamma sequences. Additional experiments were carried out to investigate the presence of clonal rearrangements of the immunoglobulin heavy chain (IgH) locus. We found clonal rearrangements of the TCR-gamma locus in 9 out of 15 lymph node biopsies. In 3 patients, the predominant cell clones carried clonal IgH and TCR-gamma rearrangements whereas 1 patient with polyclonal TCR-gamma pattern displayed IgH-monoclonality. The statistical evaluation of morphological and immunohistochemical data indicated that no single variable was able significantly to predict the clonality of the lesion. Furthermore, demonstrable clonality for the TCR-gamma or the IgH loci of a lesion did not correlate with a bad clinical course. Our data correlate with findings of other studies investigating AILD-TCL in Caucasian populations.
authors.affiliation: Department of Pathology, University of Cologne, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Lorenzen
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Zhao-Höhn
authors.forename: C
authors.initials: C
authors.lastname: Wintzer
authors.forename: R
authors.initials: R
authors.lastname: Fischer
authors.forename: M L
authors.initials: ML
authors.lastname: Hansmann
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D002681:China; D002999:Clone Cells; D005260:Female; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000150:complications / Q000235:genetics / Q000473:pathology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000150:complications / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D007143:Immunoglobulin Heavy Chains; D016692:Receptors, Antigen, T-Cell, gamma-delta