Lipid peroxidation and antioxidant enzymes in vanadate-treated rats.
Male Wistar rats received an aqueous solution of ammonium metavanadate (AMV) of 0.15 mg/V/ml concentration instead of water for 14 days. The erythrocyte count and haemoglobin level in blood were not changed; the haematocrit index was slightly increased. The spontaneous lipid peroxidation in kidney and liver homogenates was increased. The Fe(II)- or ascorbate-induced lipid peroxidation was more pronounced in the kidney than in the liver. No changes in lipid peroxidation were observed in erythrocytes after AMV treatment. The AMV treatment resulted in a decrease in the activity of the antioxidant enzymes, catalase and glutathione peroxidase in the kidney and liver; the cytosolic Cu,Zn-SOD and mitochondrial Mn-SOD were unchanged. The activity of the enzymes in blood was not changed. The results are discussed with a view to the participation of lipid peroxidation in vanadium toxicity.
Russanov
E
E
Institute of Physiology, Bulgarian Academy of Sciences, Sofia.
Zaporowska
H
H
Ivancheva
E
E
Kirkova
M
M
Konstantinova
S
S
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D004906:Erythrocyte Count; D005502:Food; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014638:Vanadates / Q000633:toxicity*; D014867:Water
D000975:Antioxidants; D006454:Hemoglobins; D014867:Water; D014638:Vanadates; D008315:Malondialdehyde; D010088:Oxidoreductases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase