[The isolation of a reassortant strain of the A/Kiev/59/79, R (H1N1) influenza virus and its genomic characteristics].
As a result of two successive recombinations of influenza A viruses: A/Leningrad/0139/76 (H3N2) with A/PR/8/34 (H1N1) strain and the resulting reassortant A/Leningrad/0139/76, R (H3N2) with a field isolate A/Kiev/59/79 (H1N1), a virus. A/Kiev/59/79, R (H1N1) was obtained. It was established by electrophoresis in polyacrylamide gel and sequencing of kDNA fragments of its genome that the genes PB2, PB1 and NP of this virus belonged to serosubtype H3N2, while genes PA, M, and NS to serosubtype H1N1 (like A/PR/8/34). The surface glycoproteins HA and NA belonged to serosubtype H1N1/like A/USSR/90/77.
Ermachenko
T A
TA
Gorev
N E
NE
Iukhnova
L G
LG
Petrov
N A
NA
Golubev
D B
DB
D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification*; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D004279:DNA, Viral
Poluchenie reassortantnogo shtamma virusa grippa A/Kiev/59/79, R (H1N1) i kharakteristika ego genoma.