Identification of a Xenopus cDNA that prevents mitotic catastrophe in the fission yeast Schizosaccharomyces pombe.
A Xenopus total ovary cDNA library was constructed in a fission yeast expression vector. Using a genetic functional complementation method, we have identified a Xenopus cDNA clone that can rescue several different yeast mitotic catastrophe mutants defective in Wee1 kinase function at the restrictive temperature. The 3.0-kb cDNA clone contains an open reading frame (ORF) of 2226 nucleotides, encoding a predicted 82-kDa protein. The deduced amino acid (aa) sequence shows seven almost identical 30-aa tandem repeats, each of which contains a phosphorylation site meeting the consensus for both Cdc2 kinase and MAP kinase.
Su
J Y
JY
Howard Hughes Medical Institute, University of Colorado School of Medicine, Denver 80262.
Maller
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016203:CDC2 Protein Kinase / Q000235:genetics; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D004247:DNA; D018076:DNA, Complementary; D005260:Female; D008938:Mitosis / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010053:Ovary / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins; D014981:Xenopus; D029867:Xenopus Proteins
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D009687:Nuclear Proteins; D011506:Proteins; D029702:Schizosaccharomyces pombe Proteins; C088622:XPMC1 protein, Xenopus; D029867:Xenopus Proteins; D004247:DNA; D011494:Protein Kinases; C530005:WEE1 protein, Xenopus; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases; D016203:CDC2 Protein Kinase