Some factors inhibiting amplification of the Staphylococcus aureus enterotoxin C1 gene (sec+) by PCR.
PCR amplification of the sec+ gene for staphylococcal enterotoxin C1 (SEC1) can be achieved from as little as 10 fg total genomic DNA (equivalent to less than 10 cells) using two nested primer pairs. The presence of bacterial cells, particularly thermonuclease-producing staphylococci, and the thermonuclease enzyme (TNase) itself, were found to be factors which individually and together reduced the sensitivity of PCR amplification.
Wilson
I G
IG
Food Microbiology Research Division, Queen's University Belfast, Northern Ireland, UK.
Cooper
J E
JE
Gilmour
A
A
D004269:DNA, Bacterial / Q000187:drug effects; D004768:Enterotoxins / Q000235:genetics*; D005798:Genes, Bacterial / Q000187:drug effects* / Q000235:genetics; D008836:Micrococcal Nuclease / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000187:drug effects*; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D004768:Enterotoxins; C034959:enterotoxin C, staphylococcal; D008836:Micrococcal Nuclease