title: Fc gamma-receptor activity of placental annexin II.
abstract: We have previously produced a MoAb, B1D6, against a placental FcR. The antigen isolated using F(ab')2-fragments of B1D6 exhibits Fc-binding properties with low affinity for IgG. The antigen is a single-chained glycoprotein with a molecular weight of approximately 37 kDa and a pI of about 7.0-8.5. Amino acid sequences from enzymatic digests of the antigen indicated that it is annexin II. Immunoreactivity using anti-annexin antisera and purified placental annexin II have further established the specificity of B1D6 to annexin II. The B1D6 epitope appears to be intramembraneous and intracellular on placental syncytiotrophoblasts, monocytes and other cells investigated.
authors.affiliation: Department of Microbiology and Immunology, Gade Institute, University of Bergen, Norway.
authors.forename: E K
authors.initials: EK
authors.lastname: Kristoffersen
authors.forename: E
authors.initials: E
authors.lastname: Ulvestad
authors.forename: L
authors.initials: L
authors.lastname: Bjørge
authors.forename: A
authors.initials: A
authors.lastname: Aarli
authors.forename: R
authors.initials: R
authors.lastname: Matre
mesh_terms: D000595:Amino Acid Sequence; D017306:Annexin A2 / Q000032:analysis / Q000502:physiology*; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D015153:Blotting, Western; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D010920:Placenta / Q000276:immunology*; D011233:Precipitin Tests; D011247:Pregnancy; D017452:Receptors, IgG / Q000502:physiology*; D012397:Rosette Formation; D014162:Transfection
chemical_list: D017306:Annexin A2; D000911:Antibodies, Monoclonal; D017452:Receptors, IgG