Expression of beta-galactosidase and luciferase in insect cell lines infected with a recombinant AcMNPV.
An AcMNPV recombinant (Ac-gal-luc) carrying the beta-galactosidase and luciferase genes under the control of the p10 and polyhedrin promoters, respectively, was used to study expression in nine insect cell lines. All AcMNPV-permissive cell lines expressed both reporter genes with the coleopteran cell line, Anthonomus grandis (AGE), producing the highest concentrations of beta-galactosidase (5.0 x 10(6) pg/ml) and luciferase (2.67 x 10(3) pg/ml). Both enzymes were detected as early as 12 h postinoculation in lysate samples of the AGE cell line. Helicoverpa armigera (HA), a nonpermissive cell line, expressed beta-galactosidase at 72 h postinoculation at a concentration of 3.5 x 10(3) pg/ml. However, expression of luciferase was not detected. Expression of luciferase and beta-galactosidase was also not detected in the nonpermissive Helicoverpa zea (HZ) cell line.
McIntosh
A H
AH
USDA, ARS Biological Control of Insects Research Laboratory, Columbia, Missouri 65205.
Grasela
J J
JJ
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D001517:Coleoptera / Q000166:cytology* / Q000382:microbiology; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D005822:Genetic Vectors; D007915:Lepidoptera / Q000166:cytology* / Q000382:microbiology; D008156:Luciferases / Q000235:genetics*; D011995:Recombination, Genetic; D001616:beta-Galactosidase / Q000235:genetics*
D008156:Luciferases; D001616:beta-Galactosidase