Evidence that Escherichia coli isolated from the intestine of healthy pigs hybridize with LT-II, ST-Ib and SLT-II DNA probes.
A DNA probe specific for genes coding for the heat-labile toxin type II (LT-II), heat-stable toxin type Ib (ST-Ib) and Shiga-like toxin type II (SLT-II), were used to examine 118 fecal Escherichia coli strains isolated from healthy pigs. Fifty-six (47.4%) of the isolated were hybridized with the LT-II probe. Thirty-nine strains (33%) possessed ST-Ib genes and five of the 118 isolates (4.2%) showed homology with the SLT-II DNA probe. E. coli that possessed unusual toxin genes for pigs were isolated with a high frequency, which indicates the importance of toxigenic clones found in nature. Uncommon virulence factors should be examined in order to improve the efficiency of diagnosis and control procedures.
Celemin
C
C
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, Spain.
Anguita
J
J
Naharro
G
G
Suarez
S
S
D000375:Aging; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D006262:Health; D009693:Nucleic Acid Hybridization; D012007:Rectum / Q000382:microbiology*; D022641:Shiga Toxin 2; D013552:Swine / Q000382:microbiology*; D014886:Weaning
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022641:Shiga Toxin 2; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli