title: Common structural features of the Ro RNP associated hY1 and hY5 RNAs.
abstract: The secondary structures of human hY1 and hY5 RNAs were determined using both chemical modification techniques and enzymatic structure probing. The results indicate that both for hY1 and for hY5 RNA the secondary structure largely corresponds to the structure predicted by sequence alignment and computerized energy-minimization. However, some important deviations were observed. In the case of hY1 RNA, two regions forming a predicted helix appeared to be single-stranded. Furthermore, the pyrimidine-rich region of hY1 RNA appeared to be very resistant to reagents under native conditions, although it was accessible to chemical reagents under semi-denaturing conditions. This may point to yet unidentified tertiary interactions for this region of hY1 RNA. In the case of hY5 RNA, two neighbouring internal loops in the predicted structure appeared to form one large internal loop.
authors.affiliation: Department of Biochemistry, University of Nijmegen, The Netherlands.
authors.forename: C W
authors.initials: CW
authors.lastname: van Gelder
authors.forename: J P
authors.initials: JP
authors.lastname: Thijssen
authors.forename: E C
authors.initials: EC
authors.lastname: Klaassen
authors.forename: C
authors.initials: C
authors.lastname: Sturchler
authors.forename: A
authors.initials: A
authors.lastname: Krol
authors.forename: W J
authors.initials: WJ
authors.lastname: van Venrooij
authors.forename: G J
authors.initials: GJ
authors.lastname: Pruijn
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015347:RNA Probes; D012335:RNA, Ribosomal / Q000737:chemistry* / Q000378:metabolism; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D000099207:SS-A Antigen
chemical_list: D001324:Autoantigens; D004247:DNA; D015347:RNA Probes; D012335:RNA, Ribosomal; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human