Messenger RNA of G-proteins alpha-subunit in rat brown adipose tissue.
The present study was addressed to quantify the steady-state mRNA levels for the alpha subunit of stimulatory (Gs) and inhibitory (Gi-1 and Gi-2) G-proteins in brown (interscapular) male rat adipose tissue (n = 6 rats). The quantification of specific mRNA, estimated using a solution hybridization RNAse protection assay, showed that the amounts of G alpha i-1, G alpha i-2 and G alpha s mRNA were 0.88 +/- 0.28 amol/microgram DNA, 76 +/- 4 amol/micrograms DNA and 460 +/- 16 amol/micrograms DNA, respectively. When the amounts of G alpha i-1 and G alpha i-2 and G alpha s mRNA in brown adipose tissue were compared with those in epididymal white adipose tissue (obtained from the same rats), G alpha i-1 and G alpha i-2 mRNA levels were very similar between brown and white adipose tissue, whereas the level of G alpha s mRNA was significantly higher in brown than in white fat tissue (P < 0.001). In conclusion, the present study shows the steady-state levels of mRNA for the alpha subunit of Gs, Gi-1 and Gi-2 in rat brown fat and suggests that the quantity of G alpha s mRNA is higher in brown than in white adipose tissue. Further studies are needed to explain the possible physiological importance of these findings.
De Pergola
G
G
Istituto di Clinica Medica, Endocrinologia e Malattie Metaboliche dell'Università di Bari, Policlinico di Bari, Italy.
Xu
X
X
Carlsson
B
B
Eriksson
P
P
Giorgino
R
R
Bjorntorp
P
P
D000273:Adipose Tissue / Q000737:chemistry; D002001:Adipose Tissue, Brown / Q000737:chemistry*; D000818:Animals; D004247:DNA / Q000032:analysis; D019204:GTP-Binding Proteins / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism
D012333:RNA, Messenger; D004247:DNA; D012260:Ribonucleases; D019204:GTP-Binding Proteins