Differential inhibition of eukaryotic DNA polymerases by halenaquinol sulfate, a p-hydroquinone sulfate obtained from a marine sponge.
Halenaquinol sulfate, a p-hydroquinone sulfate obtained from a marine sponge, inhibited the activity of eukaryotic DNA polymerases in varying degrees; the Ki values for DNA polymerases, alpha, beta, delta and epsilon were 1.3, 80, 17.5 and 2.0 microM, respectively, whereas it was less effective against E. coli DNA polymerase I. The inhibition occurred competitively with each of dATP and dTTP, but non-competitively with dCTP, dGTP and the template DNA. Thus, halenaquinol sulfate is demonstrated to be a potential inhibitor of DNA polymerases alpha and epsilon, and be a useful tool for analyzing the dNTP binding sites of DNA polymerases.
Shioda
M
M
Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Japan.
Kano
K
K
Kobayashi
M
M
Kitagawa
I
I
Shoji
M
M
Yoshida
S
S
Ikegami
S
S
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D001551:Benz(a)Anthracenes / Q000494:pharmacology*; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D006493:Heparin / Q000494:pharmacology; D006873:Hydroquinones / Q000494:pharmacology*; D007700:Kinetics; D019384:Nucleic Acid Synthesis Inhibitors; D011161:Porifera / Q000737:chemistry
D001551:Benz(a)Anthracenes; D006873:Hydroquinones; D019384:Nucleic Acid Synthesis Inhibitors; D016590:Aphidicolin; D006493:Heparin; C074982:halenaquinol sulfate; D004257:DNA Polymerase II