Effect of lipid peroxidation on beating frequency of human sperm tail.
The tail beat frequency (TBF) of sperm was measured with a sperm-head fixation method which was based on the tendency of sperm to attach its head to the surface of a glass slide. Ferrous sulphate, a promotor of lipid peroxidation, inhibited TBF within 5 min of mixing with sperm. This inhibition can be reversed dose-dependently by superoxide dismutase, catalase, glutathione, and albumin. It was concluded that TBF could be a new pharmacological model for studying the effect of lipid peroxidation and antioxidant on sperm motility. Both enzymatic and non-enzymatic antioxidants can be screened with this method.
Hong
C Y
CY
Institute of Traditional Medicine, National Yang-Ming Medical College, Taipei, Taiwan, ROC.
Lee
M F
MF
Lai
L J
LJ
Wang
C P
CP
D002374:Catalase / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013082:Sperm Tail / Q000502:physiology*; D013482:Superoxide Dismutase / Q000494:pharmacology
D005296:Ferrous Compounds; D012710:Serum Albumin, Bovine; C020748:ferrous sulfate; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione