Interleukin-1 receptor deficiency in brains from NZB and (NZB/NZW)F1 autoimmune mice.
Interleukin-1 receptors (IL-1R) are expressed in the brain and the anterior pituitary of normal mice (C3H/He, Swiss), and appear to be involved in the neuroendocrine control of the immune response. Here we have studied the IL-1R density in the brain and the pituitary from several strains of autoimmune mice (NZB, (NZB/NZW)F1, MRL/MP-lpr), using quantitative autoradiography with recombinant human [125I]IL-1 alpha as a ligand. IL-1R was similar in the brain of C3H/He, Swiss and NZW (controls) and MRL/MP-lpr mice. In NZB mice a profound deficit (10% of control mice) in IL-1R was observed exclusively in the dentate gyrus. In (NZB/NZW)F1 the deficit was about 50%. These observations were independent of sex and age. Pituitary receptors were not affected in all the strains except NZW (30% increase). Competition experiments demonstrated that the affinity of IL-1R was not modified in dentate gyrus of (NZB/NZW)F1 and NZW mice. Thus, the number of IL-1R was the only parameter affected. This deficit was not reversed by corticosterone treatment (0.2 mg/20 g body weight, i.p.) and was poorly modified by lipopolysaccharide treatment (0.1 mg/20 g body weight, i.p.) compared to C3H/He mice. In conclusion, this central IL-1R deficit is unlikely to be the consequence of occupancy by abnormal synthesis of brain IL-1. This abnormality is tissue-specific with hereditary autosomal transmission. The role of central IL-1R in neuroimmunoendocrine interactions and in autoimmunity remains to be clarified.
Tehrani
M J
MJ
Institut Pasteur, Paris, France.
Hu
Y
Y
Marquette
C
C
Dietrich
H
H
Haour
F
F
Wick
G
G
D000367:Age Factors; D000818:Animals; D001327:Autoimmune Diseases / Q000378:metabolism*; D001923:Brain Chemistry; D003345:Corticosterone / Q000494:pharmacology; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008814:Mice, Inbred NZB; D010902:Pituitary Gland / Q000737:chemistry; D017472:Receptors, Interleukin-1 / Q000032:analysis* / Q000502:physiology; D012737:Sex Factors
D008070:Lipopolysaccharides; D017472:Receptors, Interleukin-1; D003345:Corticosterone