Woodchuck hepatitis virus surface antigen produced in vitro fails to bind polymerized woodchuck serum albumin.
Using a plasmid (pSWS) similar to one that has been successfully used for large-scale production of hepatitis B virus (HBV) envelope protein particles (pSVS) but containing the corresponding woodchuck hepatitis virus (WHV) envelope gene sequences, we have stably transformed the rodent dihydrofolate reductase-deficient cell line CHO dhfr-. Although production of WHV envelope particles in CHO/pSWS cell lines was low, it was sufficient to test whether these particles could bind to polymerized serum albumin. Whereas binding of HBV particles produced in CHO/pSVS cells to polymerized human serum albumin could readily be detected, we found no evidence that the WHV envelope protein particles produced in vitro bind to either human or woodchuck polymerized serum albumin.
Shamoon
B
B
UPR 41, CNRS, Centre Hayem, Hôpital St. Louis, Paris, France.
Mandart
E
E
Kay
A
A
Galibert
F
F
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D017919:Hepatitis B Virus, Woodchuck / Q000276:immunology*; D011994:Recombinant Proteins / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism*; D000075462:Serum Albumin, Human; D013045:Species Specificity; D014170:Transformation, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
D000956:Antigens, Viral; D011994:Recombinant Proteins; D012709:Serum Albumin; D014759:Viral Envelope Proteins; C032137:polyalbumin; D000075462:Serum Albumin, Human