Prolongation of the intravascular retention of hemoglobin modified with a long-chain fatty acid derivative.
To develop hemoglobin (Hb) derivatives with an increased circulatory half-life, Hb was chemically modified with long chain fatty acid analogs. One compound, sodium 1-hexadecyl 6-(2-iodoacetamido)hexyl phosphate, specifically modified the Cys-93 beta residues of human hemoglobin (HbA) as determined by sulfhydryl titration analysis. The resulting modified Hb derivative, FAHbA, was isolated and was shown to have a two-fold longer circulatory half-life than native HbA in a rat low-dose acute transfusion model.
Fowler
S A
SA
Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242.
Andracki
M
M
Hurst
G
G
Honkan
V A
VA
Walder
J
J
Casteel
D A
DA
D000818:Animals; D001803:Blood Transfusion; D001808:Blood Vessels; D003545:Cysteine / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D006207:Half-Life; D006454:Hemoglobins / Q000737:chemistry* / Q000493:pharmacokinetics*; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000097:blood; D013997:Time Factors; D017186:Titrimetry
D005227:Fatty Acids; D006454:Hemoglobins; D013438:Sulfhydryl Compounds; D003545:Cysteine