Immunohistochemical localization of GnRH in the crested newt (Triturus carnifex) brain and terminal nerve: a developmental study.
Localization of GnRH-immunoreactive neuronal system was studied by immunohistochemistry in the nasal-brain area of the crested newt, Triturus carnifex. Besides adults, developmental stages were those from hatchlings up to complete metamorphosis. Neurons containing immunoreactive GnRH were first detected in the nasal area of larvae with yet undifferentiated gonads. Subsequently, in prometamorphic stages, GnRH-immunoreactive cell bodies and fibers were detected in the proximal part of the terminal nerve as well as along the ventromedial surface of the olfactory bulbs. In older larvae with sexually differentiated gonads and up to the metamorphic climax GnRH-neurons were detected, as a rostral to caudal continuum, along the ventromedial surface of the olfactory bulbs and midtelencephalon. This is exactly the route followed by the terminal nerve. In the adult brain, besides the presence of occasional GnRH-neurons and fibers in the terminal nerve proximal to olfactory bulbs, olfactory bulbs and the mid-basal telencephalon, another aggregate of immunoreactive neurons was present in the anterior preoptic area, and a greater number of fibers in the habenular area as well as in the infundibular floor, median eminence and pars nervosa. These data suggest the nasal area to forebrain migration (along the course of the terminal nerve) of GnRH-neurons during development in the crested newt.
D'Aniello
B
B
Department of Zoology, University of Naples, Italy.
Masucci
M
M
di Meglio
M
M
Iela
L
L
Rastogi
R K
RK
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009830:Olfactory Bulb / Q000166:cytology / Q000502:physiology; D010525:Peripheral Nerves / Q000166:cytology / Q000502:physiology; D011301:Preoptic Area / Q000166:cytology / Q000502:physiology; D012727:Sex Characteristics; D013687:Telencephalon / Q000166:cytology / Q000502:physiology; D014319:Triturus
D007987:Gonadotropin-Releasing Hormone