Dual regulation by opioids of 3H-norepinephrine release in the human neuroblastoma cell-line SK-N-SH.
Depolarization-evoked 3H-norepinephrine release from SK-N-SH cells was found to be regulated by opioid ligands. Opioids exerted either inhibition or augmentation of 3H-norepinephrine release. Both effects were mediated by opioid receptors. In addition, a nonopioid inhibitory effect of opiates on release was observed. The SK-N-SH cell-line provides a suitable model for studying the various mechanisms underlying the opioid regulatory pathways within single cells.
Keren
O
O
Clinical Pharmacology Unit-Medicine F, Beilinson Medical Center, Petah-Tiqva, Israel.
Garty
M
M
Sarne
Y
Y
D002460:Cell Line; D003917:Dextrorphan / Q000494:pharmacology; D004347:Drug Interactions; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D020881:Enkephalin, D-Penicillamine (2,5)-; D004745:Enkephalins / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D007981:Levorphanol / Q000494:pharmacology; D009020:Morphine / Q000494:pharmacology; D009270:Naloxone / Q000494:pharmacology; D009294:Narcotics / Q000494:pharmacology*; D009447:Neuroblastoma / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D011957:Receptors, Opioid / Q000187:drug effects / Q000502:physiology; D014316:Tritium; D014407:Tumor Cells, Cultured
D004745:Enkephalins; D009294:Narcotics; D011957:Receptors, Opioid; D003917:Dextrorphan; D014316:Tritium; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D007981:Levorphanol; D009270:Naloxone; D009020:Morphine; D020881:Enkephalin, D-Penicillamine (2,5)-; D009638:Norepinephrine