Development of an enzyme-mediated assay for phenylalanine in blood spots.
We describe the optimization of a rapid procedure for the elution of phenylalanine from blood spots and the estimation of the amino acid eluted using an enzyme-mediated assay linked to a colorimetric detection system. The method is rapid, fully quantitative, interference-free, accurate and precise unlike many of the methods currently employed for phenylalanine determination. The performance of the proposed method may necessitate the review of reference ranges and cut-off assignment strategies.
Campbell
R S
RS
Department of Clinical Biochemistry, London Hospital Medical College, UK.
Hollifield
R D
RD
Varsani
H
H
Milligan
T P
TP
Brearley
G
G
Price
C P
CP
D000328:Adult; D000594:Amino Acid Oxidoreductases; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D006801:Humans; D010649:Phenylalanine / Q000097:blood*; D012016:Reference Values; D015203:Reproducibility of Results; D013696:Temperature
D010649:Phenylalanine; D000594:Amino Acid Oxidoreductases; C022342:phenylalanine oxidase