Further characterization of the pituitary-adrenocortical responses to stress in Chiroptera.
Previous studies on bats from this laboratory have revealed the presence of exceptionally high circulating levels of glucocorticoids in two species of the sub-order Megachiroptera. In the present study, the following questions were asked: (1) what effect does the routine handling and examination of captive bats have on the activity of their hypothalamic-pituitary-adrenocortical (HPA) axis?; (2) are the unusually high plasma levels of cortisol and corticosterone found in Pteropus hypomelanus associated with high levels of circulating adrenocorticotropic hormone (ACTH)?; (3) are there diurnal changes in stress responsivity in this species?; and (4) how do levels of glucocorticoids in P. hypomelanus compare with those found in other species of Chiroptera (both micro and megachiropteran species)? Of five species examined, P. hypomelanus had slightly higher total glucocorticoid levels than P. pumulis, but approximately 8-fold higher levels than in three species of Microchiroptera (Artibeus jamaicensis, Carollia perspicillata, and Myotis lucifigus). There was a pronounced diurnal rhythm in glucocorticoid levels in one species (M. lucifigus) for which this was determined. A 1-h period of restraint stress increased glucose and glucocorticoid levels in P. pumulis, and also increased ACTH and glucocorticoids in P. hypomelanus. Fifteen minutes of routine handling (weighting, measuring, etc.) elicited a significant rise in plasma glucocorticoids in P. hypomelanus to combined peak (cortisol plus corticosterone) levels of over 1,000 ng/ml (100 micrograms%). There was no significant difference in the response to handling in bats tested in the morning or evening. Basal ACTH levels as detected by radioimmunoassay were low in P. hypomelanus, in spite of high steroid levels.(ABSTRACT TRUNCATED AT 250 WORDS)
Widmaier
E P
EP
Department of Biology, Boston University, Massachusetts 02215.
Harmer
T L
TL
Sulak
A M
AM
Kunz
T H
TH
D000324:Adrenocorticotropic Hormone / Q000097:blood* / Q000378:metabolism; D000818:Animals; D000830:Animals, Laboratory; D002685:Chiroptera / Q000502:physiology*; D002940:Circadian Rhythm; D003345:Corticosterone / Q000097:blood* / Q000378:metabolism; D005260:Female; D006234:Handling, Psychological; D006854:Hydrocortisone / Q000097:blood* / Q000378:metabolism; D008297:Male; D010913:Pituitary-Adrenal System / Q000502:physiology* / Q000503:physiopathology; D012149:Restraint, Physical; D013045:Species Specificity; D013315:Stress, Psychological / Q000097:blood / Q000503:physiopathology*
D000324:Adrenocorticotropic Hormone; D003345:Corticosterone; D006854:Hydrocortisone