A dioxygenase gene (Ids2) expressed under iron deficiency conditions in the roots of Hordeum vulgare.
A lambda zapII cDNA library was constructed from mRNA isolated from Fe-deficient barley roots and screened with cDNA probes made from mRNA of Fe-deficient and Fe-sufficient (control) barley roots. Seven clones were selected. Among them a clone having the putative full-length mRNA of dioxygenase as judged by northern hybridization was selected and named Ids2 (iron deficiency-specific clone 2). Using a cDNA fragment as probe, two clones from the genomic library (lambda EMBL-III) were isolated and one was sequenced. The predicted amino acid sequence of Ids2 resembled that of 2-oxoglutarate-dependent dioxygenase. Ids2 is expressed in the Fe-deficient barley roots but is not in the leaves. The expression is repressed by the availability of Fe. Ids2 was also strongly expressed under Mn deficiency and weakly under Zn deficiency or excess NaCl (0.5%). The upstream 5'-flanking region of Ids2 has a root-specific cis element of the CaMV 35S promoter and a nodule-specific element of leghemoglobin, a metal regulatory element (MRE) and several Cu regulatory elements (UAS) of yeast metallothionein (CUP1).
Okumura
N
N
Laboratory of Plant Nutrition and Fertilizer, University of Tokyo, Japan.
Nishizawa
N K
NK
Umehara
Y
Y
Ohata
T
T
Nakanishi
H
H
Yamaguchi
T
T
Chino
M
M
Mori
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D016384:Consensus Sequence; D015870:Gene Expression / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015723:Gene Library; D017343:Genes, Plant / Q000187:drug effects*; D001467:Hordeum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D007501:Iron / Q000494:pharmacology*; D007874:Leghemoglobin / Q000096:biosynthesis; D008668:Metallothionein / Q000096:biosynthesis; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D007874:Leghemoglobin; D010940:Plant Proteins; D012333:RNA, Messenger; D008668:Metallothionein; D007501:Iron; D006899:Mixed Function Oxygenases; C088978:Ids2 protein, Hordeum vulgare