A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators.
Our investigations of mammalian class II gene transcription resulted in identification, purification, and cloning of the corresponding cDNA of a cellular factor (p15) that mediates the effects of several distinct activators on transcription in vitro. Functional deletion analyses revealed a bipartite structure of p15 comprising an amino-terminal regulatory domain and a carboxy-terminal cryptic DNA-binding domain. We provide evidence that activity of p15 is controlled by protein kinases that target the regulatory domain. Structural and functional similarities, including sequence homology to domains essential for cofactor function, cofactor activity, promiscuity with respect to transcriptional activators, and interactions with components of the basal transcription machinery, relate this novel cellular cofactor to viral immediate-early transcriptional regulators.
Kretzschmar
M
M
Laboratorium für Molekulare Biologie-Genzentrum, Ludwig-Maximilians-Universität München, Martinsried, Federal Republic of Germany.
Kaiser
K
K
Lottspeich
F
F
Meisterernst
M
M
D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D006564:Herpesviridae / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins
C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D017874:Immediate-Early Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D011494:Protein Kinases; D012319:RNA Polymerase II