title: Endogenous synthesis of N-methylnorsalsolinol in rat brain during in vivo microdialysis with epinine.
abstract: The in vivo metabolic pathway for the synthesis of N-methylnorsalsolinol, an analogue of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), was studied in the rat brain. N-Methyldopamine (epinine) was perfused at the striatum of the rat brain by in vivo microdialysis. N-Methylnorsalsolinol (NMNSAL) was identified in the brain dialysate after epinine perfusion using gas chromatography-selected-ion monitoring mass spectrometry (GC-SIM-MS). We demonstrated that NMNSAL could be synthesized from epinine with an aldehyde by the Piclet-Spengler condensation reaction in the rat brain.
authors.affiliation: Department of Pediatrics, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kajita
authors.forename: T
authors.initials: T
authors.lastname: Niwa
authors.forename: W
authors.initials: W
authors.lastname: Maruyama
authors.forename: D
authors.initials: D
authors.lastname: Nakahara
authors.forename: N
authors.initials: N
authors.lastname: Takeda
authors.forename: H
authors.initials: H
authors.lastname: Yoshizumi
authors.forename: A
authors.initials: A
authors.lastname: Tatematsu
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Naoi
authors.forename: T
authors.initials: T
authors.lastname: Nagatsu
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry; D003846:Deoxyepinephrine / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007546:Isoquinolines / Q000032:analysis / Q000138:chemical synthesis / Q000378:metabolism*; D017551:Microdialysis; D051381:Rats; D017208:Rats, Wistar; D044005:Tetrahydroisoquinolines
chemical_list: D000447:Aldehydes; D007546:Isoquinolines; D044005:Tetrahydroisoquinolines; C069194:2-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline; D003846:Deoxyepinephrine