Proteinase K and T4 DNA polymerase facilitate the blunt-end subcloning of PCR products.
Hitti
Y S
YS
Division of Biology and Medicine, Brown University, Providence, RI 02912.
Bertino
A M
AM
D004259:DNA-Directed DNA Polymerase; D019286:Endopeptidase K; D016133:Polymerase Chain Reaction / Q000379:methods*; D012697:Serine Endopeptidases / Q000494:pharmacology*; D014764:Viral Proteins / Q000494:pharmacology*
D014764:Viral Proteins; C069983:gene 43 protein, Enterobacteria phage T4; D004259:DNA-Directed DNA Polymerase; D012697:Serine Endopeptidases; D019286:Endopeptidase K