Gene targeting by a vector with hairpin-shaped oligonucleotide caps.
To improve the efficiency of gene targeting, a linearized vector containing part of the mouse transthyretin (ttr) gene, a bacterial neomycin-resistance gene and a herpes simplex virus thymidine kinase gene was capped with hairpin-shaped oligonucleotides. The capped vector not only improved the efficiency of positive-negative selection about 3.5-fold, but also increased about 2.5-fold the ratio of ttr gene targeted clones to the selectants.
Horie
K
K
Department of Medical Genetics, Osaka University, Japan.
Shimada
K
K
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011228:Prealbumin / Q000235:genetics; D018139:Simplexvirus / Q000201:enzymology; D013234:Stem Cells / Q000166:cytology; D013937:Thymidine Kinase / Q000235:genetics; D014407:Tumor Cells, Cultured
D009838:Oligodeoxyribonucleotides; D011228:Prealbumin; D013937:Thymidine Kinase