title: Electrostatic attraction by surface charge does not contribute to the catalytic efficiency of acetylcholinesterase.
abstract: Acetylcholinesterases (AChEs) are characterized by a high net negative charge and by an uneven surface charge distribution, giving rise to a negative electrostatic potential extending over most of the molecular surface. To evaluate the contribution of these electrostatic properties to the catalytic efficiency, 20 single- and multiple-site mutants of human AChE were generated by replacing up to seven acidic residues, vicinal to the rim of the active-center gorge (Glu84, Glu285, Glu292, Asp349, Glu358, Glu389 and Asp390), by neutral amino acids. Progressive simulated replacement of these charged residues results in a gradual decrease of the negative electrostatic potential which is essentially eliminated by neutralizing six or seven charges. In marked contrast to the shrinking of the electrostatic potential, the corresponding mutations had no significant effect on the apparent bimolecular rate constants of hydrolysis for charged and non-charged substrates, or on the Ki value for a charged active center inhibitor. Moreover, the kcat values for all 20 mutants are essentially identical to that of the wild type enzyme, and the apparent bimolecular rate constants show a moderate dependence on the ionic strength, which is invariant for all the enzymes examined. These findings suggest that the surface electrostatic properties of AChE do not contribute to the catalytic rate, that this rate is probably not diffusion-controlled and that long-range electrostatic interactions play no role in stabilization of the transition states of the catalytic process.
authors.affiliation: Department of Biochemistry, Israel Institute for Biological Research, Ness-Ziona.
authors.forename: A
authors.initials: A
authors.lastname: Shafferman
authors.forename: A
authors.initials: A
authors.lastname: Ordentlich
authors.forename: D
authors.initials: D
authors.lastname: Barak
authors.forename: C
authors.initials: C
authors.lastname: Kronman
authors.forename: R
authors.initials: R
authors.lastname: Ber
authors.forename: T
authors.initials: T
authors.lastname: Bino
authors.forename: N
authors.initials: N
authors.lastname: Ariel
authors.forename: R
authors.initials: R
authors.lastname: Osman
authors.forename: B
authors.initials: B
authors.lastname: Velan
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000596:Amino Acids / Q000737:chemistry; D001665:Binding Sites; D002384:Catalysis; D003196:Computer Graphics; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D015394:Molecular Structure; D016254:Mutagenesis, Insertional; D009994:Osmolar Concentration
chemical_list: D000596:Amino Acids; D000110:Acetylcholinesterase