Cloning of a VLP-protein coding gene from a parasitoid wasp Venturia canescens.
Virus-like particles (VLPs) that are bound to the egg surface of a parasitic wasp Venturia canescens are void of nucleic acid and unable to infect host tissue but instead provide a passive protection against the host's immune recognition. To investigate evolutionary and functional properties of Venturia particles, we isolated cDNAs coding for a VLP-protein by screening an expression library of the wasp, using antibodies against purified VLPs. The corresponding coding DNA is present in the wasp genome as a single-copy gene.
Theopold
U
U
Institut für Biologie III, Freiburg, Germany.
Krause
E
E
Schmidt
O
O
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D005091:Exons; D015723:Gene Library; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010447:Peptide Hydrolases; D011506:Proteins / Q000235:genetics*; D018626:Retroelements; D014863:Wasps / Q000235:genetics*
D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D011506:Proteins; D018626:Retroelements; D004247:DNA; D010447:Peptide Hydrolases; C063089:Copia protein, Drosophila