Partial characterization of a fraction from bull seminal vesicle fluid that potentiates the bull sperm acrosome reaction in vitro.
A fraction from bovine seminal vesicle fluid that initiated acrosome reaction of bovine epididymal spermatozoa in vitro in the presence of heparin was prepared by sequential chromatographies on heparin-Sepharose, gel filtration (Superose 12) and reversed phase chromatography (ProRPC). Sequence analysis of the separated fraction showed that it contained the major protein (PDC-109) with 100% homology. This fraction contained also Ca(2+)-dependent phospholipase A2-like activity which hydrolysed phosphatidylethanolamine and phosphatidylcholine with 14C-labelled linoleic (lino-PE, lino-PC) or arachidonic acid (ara-PE, ara-PC) at sn-2 position. This protein was not detected in N-terminal sequence analysis. Lysophosphatidylcholine, lysophosphatidylethanolamine, and p-bromophenacyl bromide (p-BPB) inhibited this lipolytic activity. Sulfoglycolipid (Seminolipid) had inhibitory effect at concentrations above 0.1 mM but activated slightly the enzyme at lower concentrations. Boiling destroyed acrosome initiating activity in the separated fraction.
Rönkkö
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Department of Anatomy, University of Kuopio, Finland.
Linnala-Kankkunen
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Tuhkanen
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AL
D000177:Acrosome / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006493:Heparin / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008297:Male; D008970:Molecular Weight; D010741:Phospholipases A / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D054467:Phospholipases A2; D012661:Semen / Q000737:chemistry* / Q000502:physiology*; D012669:Seminal Vesicles / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D013379:Substrate Specificity
D006493:Heparin; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium