Shuttle mutagenesis of Legionella pneumophila: identification of a gene associated with host cell cytopathicity.
We performed shuttle mutagenesis of Legionella pneumophila. Mutants were screened for reduced cellular infectivity. Approximately 10% of the mutants had decreased cytopathicity. The DNA sequence of one locus was determined; the inferred amino acid sequence revealed homology with transport proteins including Escherichia coli TolC, Bordetella pertussis CyaE, and Alcaligenes eutrophus CzcC and CnrC.
Arroyo
J
J
Department of Microbiology, University of Michigan Medical School, Ann Arbor 48109.
Hurley
M C
MC
Wolf
M
M
McClain
M S
MS
Eisenstein
B I
BI
Engleberg
N C
NC
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D006801:Humans; D016952:Legionella pneumophila / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D016296:Mutagenesis
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; C088933:Hel protein, Legionella pneumophila