Different mass spectrometric approaches in the identification of endogenous methylated purine bases in urine extracts.
A preliminary identification of endogenous and exogenous methylated purine bases in urinary extracts of healthy and tumor-bearing subjects has been performed using high performance liquid chromatography, and tandem mass spectrometry (MS/MS). MS/MS gave particularly fast and sensitive analyses, allowing the simultaneous and rapid determination of 26 different urinary methylated purines. Both sets of data confirm that tumor-bearing patients show changed levels of methylated purine bases.
Porcelli
B
B
Istituto di Biochimica ed Enzimologia, Siena, Italy.
Pagani
R
R
Lorenzini
L
L
De Martino
A
A
Catinella
S
S
Traldi
P
P
D002851:Chromatography, High Pressure Liquid; D015179:Colorectal Neoplasms / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry; D008745:Methylation; D008970:Molecular Weight; D011687:Purines / Q000652:urine*; D013056:Spectrophotometry, Ultraviolet
D011687:Purines