Neurotrophins affect survival and neuritogenesis by adult injured auditory neurons in vitro.
This study evaluates the trophic effects of three neurotrophins on traumatized adult auditory neurons in culture, and the presence of these neurotrophins in cochlear nucleus tissue. BDNF and NT-3 promoted survival but very limited neuritogenesis by adult auditory neurons in vitro, while NGF, although without a survival effect, evoked a robust neuritic outgrowth response when combined with BDNF. Messenger RNAs that encode for NGF, BDNF and NT-3 were detected by RT-PCR in RNA extracts from adult cochlear nuclei tissue. Based on these in vitro and in vivo findings, we propose NT-3 as the agent of the peripheral target-derived survival promoting effect and NGF, BDNF, and NT-3 as mediators of trophic influences originating from the central target (i.e. cochlear nucleus).
Lefebvre
P P
PP
Department of Otorhinolaryngology and Audiophonology, University of Liege, Belgium.
Malgrange
B
B
Staecker
H
H
Moghadass
M
M
Van de Water
T R
TR
Moonen
G
G
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017626:Cochlear Nucleus / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000187:drug effects* / Q000648:ultrastructure; D020933:Neurotrophin 3; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D000159:Vestibulocochlear Nerve / Q000166:cytology; D061285:Vestibulocochlear Nerve Injuries
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3