Cutinase gene disruption in Fusarium solani f sp pisi decreases its virulence on pea.
Fusarium solani f sp pisi (Nectria haematococca) isolate 77-2-3 with one cutinase gene produced 10 to 20% of the cutinase produced by isolate T-8 that has multiple cutinase genes, whereas cutinase gene-disrupted mutant 77-102 of isolate 77-2-3 did not produce cutinase. On the surface of pea stem segments, lesion formation was most frequent and most severe with T-8, less frequent and less severe with 77-2-3, and much less frequent and much milder with the gene-disrupted mutant. Microscopic examination of the lesions caused by the mutant strongly suggest that it penetrated the host mostly via the stomata. In seedling assays, 77-2-3 caused severe lesions on every seedling and stunted growth, whereas the mutant showed very mild lesions on one-third of the seedlings with no stunting. Thus, cutinase gene disruption resulted in a significant decrease in the pathogenicity of F. s. pisi on pea.
Rogers
L M
LM
Ohio State Biotechnology Center, Ohio State University, Columbus 43210-1002.
Flaishman
M A
MA
Kolattukudy
P E
PE
D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D004790:Enzyme Induction; D007887:Fabaceae / Q000254:growth & development / Q000382:microbiology*; D005670:Fusarium / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*; D005798:Genes, Bacterial; D008563:Membrane Lipids / Q000096:biosynthesis; D009154:Mutation; D010935:Plant Diseases; D010946:Plants, Medicinal; D013997:Time Factors; D014316:Tritium; D014774:Virulence / Q000235:genetics
D008563:Membrane Lipids; D014316:Tritium; C000521:cutin; D002265:Carboxylic Ester Hydrolases; C019952:cutinase