[Bacteriophage lambda:lux: design and expression of bioluminescence in E. coli cells].
The bacteriophages lambda:lux and lambda:luxAB have been constructed by ligation of phage arms generated by BamHI or SalGI restriction endonucleases digestion of EMBL4 to BamHI digested plasmid pF1 lux+ or to SalGI digested plasmid pF2 lambda:luxA+B+. Cells of Escherichia coli prototrophic strain Cs were infected with lambda:lux or lambda:luxAB and intensity of bioluminiscence of the samples registered at different time intervals determined. The signal of bioluminiscence was first detected 15 min after infection and its level increased exponentially thereafter demonstrating replication of the lambda:lux bacteriophages. We have used the recombinant lambda:luxAB bacteriophage to detect the enteric indicator bacteria without enrichment in 15 min, provided that they are present at levels higher than 10(4).
Duzhiĭ
D E
DE
Zavil'gel'skiĭ
G B
GB
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D008156:Luciferases / Q000235:genetics*; D008163:Luminescent Measurements; D010957:Plasmids
D008156:Luciferases
Bakteriofag lamda:lux: konstruirovanie i ékspressiia bioliuminestsentsii v kletkakh E. coli.