title: Different events involved in the induction of macrophage tumor necrosis factor by Candida albicans and lipopolysaccharide.
abstract: Using an in vitro experimental model, we have recently demonstrated that Candida albicans in its hyphal form (H-Candida), similarly to lipopolysaccharide (LPS), enhances tumor necrosis factor (TNF) secretory response in the cloned macrophage (M phi) population ANA-1. Here we show that H-Candida and LPS each differ in their requirements for intact protein kinase functions, susceptibility to 0.4-microns micropore-size membranes, and sensitivity to polymyxin B. These results, together with the synergistic effect occurring between H-Candida and LPS in inducing TNF response, indicate the existence of different receptor(s) and/or signal-transduction pathway(s) through which the two stimuli act.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Blasi
authors.forename: L
authors.initials: L
authors.lastname: Pitzurra
authors.forename: M
authors.initials: M
authors.lastname: Puliti
authors.forename: R
authors.initials: R
authors.lastname: Mazzolla
authors.forename: R
authors.initials: R
authors.lastname: Barluzzi
authors.forename: S
authors.initials: S
authors.lastname: Saleppico
authors.forename: F
authors.initials: F
authors.lastname: Bistoni
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000494:pharmacology; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology*; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011112:Polymyxin B / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000946:Antigens, Fungal; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D011112:Polymyxin B