The organization of the beta-globin gene of the bivalve mollusc Anadara trapezia and its evolutionary relationship to other invertebrate and vertebrate globin genes.
Three pairs of oligonucleotide primers based on partial DNA and amino acid sequences were used in a combination of PCR experiments to amplify the beta-globin gene of the bivalve mollusc Anadara trapezia. The sequence of 2,139 bp presented contains the whole of the beta-globin gene with the exception of the 5' flanking sequence. This gene possesses the three-exon-and-two-intron gene structure typical of vertebrate globin genes but the lengths of the introns (762 bp and 690 bp, respectively) are only approximately half the size of those present in a beta-variant gene previously characterized from this organism. The encoded amino acid sequence shows two changes when compared to the previously published amino acid sequence.
Nassif
N T
NT
School of Biochemistry and Molecular Genetics, University of New South Wales, Kensington, Australia.
Glenn
W K
WK
Mackinlay
A G
AG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D005091:Exons; D014644:Genetic Variation; D005914:Globins / Q000235:genetics*; D007438:Introns; D007448:Invertebrates / Q000235:genetics*; D008969:Molecular Sequence Data; D008974:Mollusca / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D014714:Vertebrates / Q000235:genetics*
D017931:DNA Primers; D005914:Globins; D004247:DNA