Regulation of D-aspartate release by glutamate and GABA receptors in cerebral cortical slices from developing and ageing mice.
The basal release of D-[3H]aspartate, an unmetabolized analogue of glutamate, from cerebral cortical slices remained at the same level from three-day-old to 24-month-old mice, but the response to K+ stimulation (50 mM) was smaller in young than in adult or aged mice. Kainate, N-methyl-D-aspartate and quisqualate (0.1 mM) stimulated the basal release of D-aspartate in the cerebral cortex of seven-day-old mice, the effects of kainate and N-methyl-D-aspartate being reduced by their antagonists 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and dizocilpine maleate, respectively, indicating that in the immature cerebral cortex the kainate and N-methyl-D-aspartate types of the glutamate receptor are involved in the basal release. The K(+)-stimulated release was not affected by glutamate agonists in developing mice, though they markedly attenuated the evoked release in adults. The inhibitory amino acids GABA, taurine and glycine depressed the K(+)-stimulated release only in the adult cerebral cortex. The action of GABA was abolished by bicuculline, demonstrating the involvement of presynaptic GABAA receptors. The glycine effect was strychnine-insensitive, characteristic of the glycine modulatory site in the N-methyl-D-aspartate receptor. This kind of regulation by both kainate and N-methyl-D-aspartate receptors could be of physiological significance, particularly in the immature cerebral cortex.
Saransaari
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P
Tampere Brain Research Center, University of Tampere, Finland.
Oja
S S
SS
D000375:Aging / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002540:Cerebral Cortex / Q000254:growth & development* / Q000378:metabolism*; D018691:Excitatory Amino Acid Antagonists; D005260:Female; D018756:GABA Antagonists; D005998:Glycine / Q000494:pharmacology; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008297:Male; D051379:Mice; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors / Q000494:pharmacology; D016318:Quisqualic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D018079:Receptors, GABA / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism; D017470:Receptors, Glutamate / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D018691:Excitatory Amino Acid Antagonists; D018756:GABA Antagonists; D018079:Receptors, GABA; D011963:Receptors, GABA-A; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D013654:Taurine; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D016202:N-Methylaspartate; D016318:Quisqualic Acid; D007608:Kainic Acid; D005998:Glycine