Determination of a new immunosuppressant, mycophenolate mofetil, and its active metabolite, mycophenolic acid, in rat and human body fluids by high-performance liquid chromatography.
Mycophenolate mofetil, a new immunosuppressant, is a morpholinoethyl ester of mycophenolic acid. A new selective, sensitive and simple high-performance liquid chromatographic method was developed for the determination of mycophenolic acid and mycophenolate mofetil in biological samples. The preparation of samples was based on liquid-liquid extraction. The compounds were separated on a CN column using acetonitrile-0.01 M phosphate buffer (1:4, v/v) as the mobile phase. UV detection was used at wavelengths 215 and 304 nm. The detection limit was 5 ng per injection volume. This method enabled pharmacokinetic and pharmacodynamic studies in humans and rats.
Sugioka
N
N
Department of Hospital Pharmacy, Kyoto Prefectural University of Medicine, Japan.
Odani
H
H
Ohta
T
T
Kishimoto
H
H
Yasumura
T
T
Takada
K
K
D000818:Animals; D001646:Bile / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004386:Duodenum; D005965:Glucuronates / Q000032:analysis / Q000097:blood; D006801:Humans; D006868:Hydrolysis; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D007441:Intubation, Gastrointestinal; D008297:Male; D009173:Mycophenolic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet
D005965:Glucuronates; D007166:Immunosuppressive Agents; D007202:Indicators and Reagents; D009173:Mycophenolic Acid