New osmoregulated beta(1-3),beta(1-6) glucosyltransferase(s) in Azospirillum brasilense.
A linear beta(1-3),beta(1-6) glucan was detected in the periplasm of Azospirillum brasilense cells growing in a medium of low osmotic strength. This glucan was produced in vitro by purified bacterial inner membranes with UDP-glucose as the sugar donor in the presence of Mg2+. Growth in a high-osmotic-strength medium strongly reduced the amount of this glucan accumulated in the periplasmic space, and the inhibition was associated with a reduction in the enzymatic activity of the beta(1-3),beta(1-6) glucosyltransferase(s).
Altabe
S G
SG
Instituto de Investigaciones Bioquímicas Fundación Campomar, Buenos Aires, Argentina.
Iñón de Iannino
N
N
de Mendoza
D
D
Ugalde
R A
RA
D015806:Azospirillum brasilense / Q000201:enzymology*; D002451:Cell Compartmentation; D005936:Glucans / Q000096:biosynthesis* / Q000737:chemistry; D005964:Glucosyltransferases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010504:Periodic Acid / Q000737:chemistry; D014882:Water-Electrolyte Balance
D005936:Glucans; D010504:Periodic Acid; D005964:Glucosyltransferases