title: Transmembrane distribution of lipophilic cations in response to an electrochemical potential in reconstituted cytochrome c oxidase vesicles and in vesicles exhibiting a potassium ion diffusion potential.
abstract: It has been shown previously that biogenic amines and a number of pharmaceutical agents can redistribute across vesicle membranes in response to imposed potassium ion or proton gradients. Surprisingly, drug accumulation is observed for vesicles exhibiting either a pH gradient (interior acidic) or a membrane potential (interior negative), implying that these compounds can traverse the lipid bilayer as either the neutral or charged species. This interpretation, however, is complicated by the fact that vesicles exhibiting a membrane potential (interior negative) accumulate protons in response to this potential, thereby creating a pH gradient (interior acidic). This raises the possibility that in both vesicle systems drug redistribution occurs in response to the proton gradient present. We have therefore compared the uptake of several lipophilic cations by reconstituted cytochrome c oxidase vesicles and by similar vesicles exhibiting a potassium ion diffusion potential. While turnover of the oxidase generates a membrane potential of comparable magnitude to the potassium ion diffusion system, it is associated with a proton gradient of opposite polarity (interior basic). Both systems show rapid uptake of the permanently charged lipophilic cation, tetraphenylphosphonium, but only the potassium ion diffusion system accumulates the lipophilic amines doxorubicin and propranolol. This provides compelling evidence that such weak bases redistribute only in response to pH gradients and not membrane potential.
authors.affiliation: Department of Pharmacology and Therapeutics, University of British Columbia, Vancouver, Canada.
authors.forename: T D
authors.initials: TD
authors.lastname: Madden
authors.forename: T E
authors.initials: TE
authors.lastname: Redelmeier
mesh_terms: D002412:Cations; D004058:Diffusion; D004317:Doxorubicin / Q000493:pharmacokinetics; D004563:Electrochemistry; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008081:Liposomes; D008564:Membrane Potentials; D009861:Onium Compounds / Q000493:pharmacokinetics; D009943:Organophosphorus Compounds / Q000493:pharmacokinetics; D011188:Potassium / Q000378:metabolism; D011433:Propranolol / Q000493:pharmacokinetics
chemical_list: D002412:Cations; D008081:Liposomes; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D004317:Doxorubicin; D011433:Propranolol; D003576:Electron Transport Complex IV; D011188:Potassium; C013289:tetraphenylphosphonium