In vivo replication of the hamster polyomavirus genome and generation of specific deletions in the process of lymphomagenesis.
Hamster polyomavirus (HaPV) causes lymphomas when injected into newborn hamsters. These tumors are virus-free but accumulate large amounts of deleted extrachromosomal viral genomes. In order to identify the major sites of virus replication in animals, we have monitored the HaPV DNA present in different organs at various times after injection. The data demonstrate that viral replication preferentially occurs in lymphoid organs. Lymphoma-associated viral genomes display specific deletions. PCR analysis shows that such viral genomes are the only variants detectable in infected animals, suggesting that they are generated by a specific cellular mechanism. We have tested the possible role of the lymphoid cell-specific V(D)J recombination activity in the generation of these specific variants. Our results indicate that this mechanism is not solely responsible for the viral genome rearrangement, if involved at all.
Mazur
S
S
Laboratoire d'Oncologie Moléculaire, Unité de Recherche Associeé 1158, Centre National de la Recherche Scientifique, Institut Gustave Roussy, Villejuif, France.
Goodhardt
M
M
Feunteun
J
J
de La Roche Saint André
C
C
D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000235:genetics; D015321:Gene Rearrangement; D008221:Lymphoid Tissue / Q000382:microbiology; D008223:Lymphoma / Q000382:microbiology*; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000254:growth & development* / Q000472:pathogenicity; D027601:Polyomavirus Infections / Q000382:microbiology*; D017384:Sequence Deletion; D014412:Tumor Virus Infections / Q000382:microbiology*; D014779:Virus Replication
D017931:DNA Primers; D004279:DNA, Viral