Human recombinant interferons-beta and -gamma decrease gelatinase production and invasion by human KG-2 renal-carcinoma cells.
We investigated the effects of human recombinant interferons (r-IFNs) on gelatinase production and invasion by human renal-cell carcinoma (HRCC). Incubation of KG-2 HRCC with human r-IFN-beta or -gamma (but not -alpha) suppressed transcription of the 72-kDa gelatinase gene and, hence, production of gelatinase activity. These inhibitory effects of interferons (IFNs) were independent of their antiproliferative effects. Treatment of KG-2 cell with r-IFN-beta or -gamma significantly inhibited cell invasion through reconstituted basement membrane toward chemoattractants produced by kidney fibroblasts. The inhibitory activity of r-IFNs was specific to the KG-2 cells since gelatinase activity by various fibroblasts was unaffected. These findings suggest that r-IFN-beta or IFN-gamma may be used to inhibit the invasive potential of HRCC.
Gohji
K
K
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Fidler
I J
IJ
Tsan
R
R
Radinsky
R
R
von Eschenbach
A C
AC
Tsuruo
T
T
Nakajima
M
M
D002292:Carcinoma, Renal Cell / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D018093:Gelatinases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D000068556:Interferon beta-1a; D000068576:Interferon beta-1b; D016899:Interferon-beta / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D007680:Kidney Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D007370:Interferon Type I; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000068576:Interferon beta-1b; D016899:Interferon-beta; D007371:Interferon-gamma; D003094:Collagen; D018093:Gelatinases; D000068556:Interferon beta-1a