title: Recovery of dominant, autosomal flightless mutants of Drosophila melanogaster and identification of a new gene required for normal muscle structure and function.
abstract: To identify further mutations affecting muscle function and development in Drosophila melanogaster we recovered 22 autosomal dominant flightless mutations. From these we have isolated eight viable and lethal alleles of the muscle myosin heavy chain gene, and seven viable alleles of the indirect flight muscle (IFM)-specific Act88F actin gene. The Mhc mutations display a variety of phenotypic effects, ranging from reductions in myosin heavy chain content in the indirect flight muscles only, to reductions in the levels of this protein in other muscles. The Act88F mutations range from those which produce no stable actin and have severely abnormal myofibrillar structure, to those which accumulate apparently normal levels of actin in the flight muscles but which still have abnormal myofibrils and fly very poorly. We also recovered two recessive flightless mutants on the third chromosome. The remaining five dominant flightless mutations are all lethal alleles of a gene named lethal(3)Laker. The Laker alleles have been characterized and the gene located in polytene bands 62A10,B1-62B2,4. Laker is a previously unidentified locus which is haplo-insufficient for flight. In addition, adult wild-type heterozygotes and the lethal larval trans-heterozygotes show abnormalities of muscle structure indicating that the Laker gene product is an important component of muscle.
authors.affiliation: Department of Biology, University of York, England.
authors.forename: R M
authors.initials: RM
authors.lastname: Cripps
authors.forename: E
authors.initials: E
authors.lastname: Ball
authors.forename: M
authors.initials: M
authors.lastname: Stark
authors.forename: A
authors.initials: A
authors.lastname: Lawn
authors.forename: J C
authors.initials: JC
authors.lastname: Sparrow
mesh_terms: D000199:Actins / Q000235:genetics; D000483:Alleles; D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005426:Flight, Animal; D005799:Genes, Dominant; D008297:Male; D008854:Microscopy, Electron; D009132:Muscles / Q000378:metabolism* / Q000648:ultrastructure; D009154:Mutation; D009218:Myosins / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D013329:Structure-Activity Relationship
chemical_list: D000199:Actins; C076399:flight muscle protein, Act88F; D009218:Myosins