title: A newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic fields.
abstract: To examine the biological effects of extremely low frequency magnetic field (ELFMF), we have designed and manufactured a new equipment for long-term and high-density exposure of cells to ELFMF. The ELFMF exposure system consists of a generator of magnets with a built-in CO2 incubator, an alternating current (AC) power supply, a gas compressor and a thermocontroller for the incubator, and a cooling unit for the magnets. The CO2 incubator made of acrylic resin is inserted into the inner-space of the silicon steel strip-cores. In this system, the temperature of the incubator is maintained at 37 +/- 0.5 degrees C. The maximum magnetic flux density on the exposure area of the incubator is 500 mT (T; tesla) at a current of 556 Arms (rms; root mean square) at 50 Hz. The long-term (up to 120 hr) exposure of 400 mT ELFMF did not affect the growth of both HL60RG and CCRF-CEM cells originated from human leukemia. The post-X-irradiation exposure of 400 mT ELFMF for 2 hr also did not affect the radiation sensitivity of GM0637 and TAT2SF cells originated from a normal human and an ataxia telangiectasia patient.
authors.affiliation: Department of Experimental Radiology, Faculty of Medicine, Kyoto University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Miyakoshi
authors.forename: S
authors.initials: S
authors.lastname: Ohtsu
authors.forename: J
authors.initials: J
authors.lastname: Tatsumi-Miyajima
authors.forename: H
authors.initials: H
authors.lastname: Takebe
mesh_terms: D002455:Cell Division / Q000528:radiation effects; D004574:Electromagnetic Fields; D004867:Equipment Design; D006801:Humans; D007938:Leukemia / Q000473:pathology; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000528:radiation effects