title: Biological factors affecting subzonal sperm microinjection results.
abstract: One hundred and sixteen couples with severe male factor infertility underwent 139 subzonal sperm microinjection cycles. In total, 1343 oocytes were microinjected, resulting in a fertilization rate of 24%, followed by a cleavage rate of 65%. In 26% of the zygotes, fertilization was delayed and embryos derived from these zygotes demonstrated a poor capacity for further growth and implantation. In 102 of 139 cycles (73%) embryo transfer was performed, resulting in 9 pregnancies. This study followed the fate of injected oocytes and early embryo development to investigate biological factors that influence the results of subzonal injection.
authors.affiliation: Reproductive Medicine Center, S.I.S.ME.R., Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Gianaroli
authors.forename: M C
authors.initials: MC
authors.lastname: Magli
authors.forename: A P
authors.initials: AP
authors.lastname: Ferraretti
authors.forename: D
authors.initials: D
authors.lastname: Fortini
authors.forename: E
authors.initials: E
authors.lastname: Feliciani
authors.forename: A
authors.initials: A
authors.lastname: Fiorentino
mesh_terms: D004624:Embryo Transfer / Q000379:methods*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods*; D015181:Gamete Intrafallopian Transfer / Q000379:methods*; D006801:Humans; D007248:Infertility, Male; D008297:Male; D008845:Microinjections; D011247:Pregnancy