High tissue content of soluble beta 1-40 is linked to cerebral amyloid angiopathy.
We developed two highly sensitive enzyme immunoassays for beta-protein with different specificities. One is specific for beta 1-40, while the other is equally sensitive to beta 1-38, beta 1-39, beta 1-40, and beta 1-42. With the enzyme immunoassays we investigated whether the soluble fraction from brain tissue contains beta 1-40 or other species of beta-protein. Aged control and Alzheimer's diseased brains showed highly variable values of beta 1-40, which was found to be the major beta species in their extracts. High tissue content of soluble beta 1-40 was not correlated to the abundance of senile plaques but was invariably associated with cerebral amyloid angiopathy. Thus, the tissue level of soluble beta 1-40 should be useful for the quantification of cerebral amyloid angiopathy.
Suzuki
N
N
Discovery Research Division, Takeda Chemical Industries, Ltd., Ibaraki, Japan.
Iwatsubo
T
T
Odaka
A
A
Ishibashi
Y
Y
Kitada
C
C
Ihara
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D016657:Cerebral Amyloid Angiopathy / Q000378:metabolism*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism*; D014020:Tissue Extracts / Q000378:metabolism
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D014020:Tissue Extracts; C078592:amyloid beta-protein (1-40)