Determination of basic drugs extracted from biological matrices by means of solid-phase extraction and wide-bore capillary gas chromatography with nitrogen-phosphorus detection.
Determination of basic drugs from biological matrices at concentration levels of 100-200 ng/mL was accomplished by using mixed-mode Bond Elut Certify columns and a gas chromatograph equipped with a nitrogen-phosphorus detector. The extraction procedure developed for general drug screening on a GC-FID system was found suitable for the extraction of basic drugs from plasma and whole blood for GC-NPD analysis. For urine samples, an extra wash step with 20% acetonitrile in water was required to obtain clean extracts. The recoveries of 18 basic drugs ranged from 77.7 to 104.5%, with relative standard deviations less than 7.0%.
Chen
X H
XH
University Center for Pharmacy, Department of Analytical Chemistry and Toxicology, Groningen, The Netherlands.
Franke
J P
JP
Wijsbeek
J
J
de Zeeuw
R A
RA
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D006801:Humans; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations / Q000032:analysis*
D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations