Randomly amplified polymorphic DNA (RAPD) and isoenzyme analysis of Trypanosoma rangeli strains.
Sixteen Trypanosoma rangeli strains were compared by isoenzyme and randomly amplified polymorphic DNA (RAPD) analysis. Eight strains were isolated from either Rhodnius prolixus or Homo sapiens from Honduras, Colombia and Venezuela. Another eight strains were isolated from either Panstrongylus megistus or the rodent Echimys dasythrix from the State of Santa Catarina, southern Brazil. All six T. rangeli strains isolated from P. megistus were co-infections with Trypanosoma cruzi, demonstrating an overlap of the sylvatic cycles of these parasites and that the accurate identification of species is of utmost importance. Both isoenzyme and RAPD analysis revealed two distinct groups of T. rangeli strains, one formed by the strains from Santa Catarina and the other, by the strains from Honduras, Colombia and Venezuela. With the five enzymes used, all the strains from Santa Catarina had identical profiles which overlapped with those of the other regions only in the pattern obtained with malic enzyme. Analysis of 138 RAPD bands by means of an unweighted pair group method analysis (UPGMA) phenogram using the Dice similarity coefficient allowed the separation of the two groups based on their divergence at a lower level of similarity than the phenon line. We show that the identification of T. cruzi and T. rangeli in naturally mixed infections is readily achieved by either RAPD or isoenzyme analysis.
Steindel
M
M
Departmento de Microbiologia e Parasitologia Universidade Federal de Santa Catarina C.P. Brazil.
Dias Neto
E
E
Pinto
C J
CJ
Grisard
E C
EC
Menezes
C L
CL
Murta
S M
SM
Simpson
A J
AJ
Romanha
A J
AJ
D000410:Alanine Transaminase / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D001483:Base Sequence; D016054:DNA, Protozoan / Q000032:analysis*; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D006721:Honduras; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis; D008969:Molecular Sequence Data; D010203:Panstrongylus; D010733:Phosphoglucomutase / Q000032:analysis; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012239:Rhodnius; D012377:Rodentia; D013020:South America; D014345:Trypanosoma / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D014352:Trypanosomiasis / Q000469:parasitology
D016054:DNA, Protozoan; D007527:Isoenzymes; D008291:Malate Dehydrogenase; C030574:malate dehydrogenase (decarboxylating); D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase