Monokines and endothelial cell proliferation in patients with diabetes mellitus.
In patients with insulin-dependent (Type 1) diabetes mellitus abnormal endothelial cell proliferation was considered as one of the possible explanations for the occurrence of retinopathy. Since monocyte-derived factors have been proposed to be involved in endothelial cell growth regulations we have measured the effect of monocyte-conditioned medium (MO-CM) on endothelial cell proliferation and the production of Tumor necrosis factor alpha and interleukin 1-beta, cytokines known to alter endothelial cell functions. The effect of MO-CM in 20 patients with Type 1 diabetes and proliferative retinopathy on endothelial cell proliferation estimated on [3H] thymidine incorporation, was lower to that observed with the MO-CM in 20 normal controls but the difference did not reach the statistical level of significance. The basal tumor necrosis factor alpha secretion expressed by monocytes was significantly lower in the MO-CM from Type 1 diabetes patients (median: 0.49, range 0.13-2.86 ng/10(6) monocytes) than in the MO-CM from the control subjects (median: 1.84, range: 0.13-7 ng-10(6) monocytes) p < 0.02. The increase in interleukin 1-beta and in tumor necrosis factor alpha production after lipopolysaccharide stimulation were similar in the 2 groups. A low basal tumor necrosis factor production per monocyte may contribute to the development of the diabetic complications as it is involved in several cellular regulation processes.
Dosquet
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Laboratoire de Biologie Vasculaire et Cellulaire, CHU Lariboisière-Saint-Louis, Paris, France.
Wautier
M P
MP
Guillausseau
P J
PJ
Wautier
J L
JL
D000293:Adolescent; D000328:Adult; D000368:Aged; D002455:Cell Division; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004247:DNA / Q000096:biosynthesis; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000276:immunology; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology*; D012016:Reference Values; D013936:Thymidine / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis*; D014471:Umbilical Veins
D017077:Culture Media, Conditioned; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D004247:DNA; D013936:Thymidine