Effects of soluble interleukin-1 receptor and tumor-necrosis factor receptor, respectively, on the IL-1- and TNF-alpha-induced DNA synthesis of acute myeloblastic leukemia blasts in vitro.
This study demonstrates that soluble interleukin-1 receptor and tumor necrosis factor receptor modulate their corresponding cytokine-induced DNA synthesis of acute myeloblastic leukemia (AML) blasts in a dose-dependent, bimodal fashion; at lower concentrations they enhanced, while at high concentrations they inhibited, the cytokine-mediated effects. Furthermore, the concentrations of endogenously produced IL-1 beta and TNF-alpha were found to be significantly (p < 0.01) higher in supernatants of AML cells cultured in the presence of corresponding soluble receptors compared to their levels in supernatants of cells growing in the absence of these molecules. Our data might suggest that the attenuation of the spontaneous decay of IL-1 beta as well as TNF-alpha activities by soluble receptors may account for their ability to augment some of their effects.
Carter
A
A
Department of Hematology, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Haddad
N
N
Draxler
I
I
Tatarsky
I
I
D000293:Adolescent; D000328:Adult; D000368:Aged; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017472:Receptors, Interleukin-1 / Q000502:physiology*; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D004273:DNA, Neoplasm; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha