Membrane-associated interleukin-1 promotes osteoclast-like cell formation in vitro.
The effect of paraformaldehyde-fixed murine macrophage P388D1 cells on osteoclast-like cell formation was investigated in mouse marrow cultures. When mouse marrow cells were cocultured for 8 days with paraformaldehyde-fixed P388D1 cells stimulated with lipopolysaccaride (LPS), many tartrate-resistant acid phosphatase (TRACP)-positive multinucleated cells were formed. Non-stimulated, and paraformaldehyde-fixed P388D1 cells did not induce the formation of TRACP-positive multinucleated cells. Salmon calcitonin and indomethacin strongly inhibited the paraformaldehyde-fixed, LPS-stimulated P388D1 cells-induced TRACP-positive multinucleated cell formation. Monospecific anti-mouse recombinant IL-1 alpha serum inhibited TRACP-positive multinucleated cell formation in the presence of paraformaldehyde-fixed P388D1 cells stimulated with LPS. These results suggest that membrane-associated IL-1 on macrophages is responsible for the resorptive effect of paraformaldehyde-fixed P388D1 cells stimulated with LPS.
Nishihara
T
T
Department of Oral Science, National Institute of Health, Tokyo, Japan.
Takahashi
T
T
Ishihara
Y
Y
Senpuku
H
H
Takahashi
N
N
Suda
T
T
Koga
T
T
D016475:3T3 Cells; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D001862:Bone Resorption / Q000209:etiology*; D002460:Cell Line; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism; D005404:Fixatives; D005557:Formaldehyde; D007106:Immune Sera / Q000276:immunology; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology*; D007527:Isoenzymes / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D010010:Osteoclasts / Q000166:cytology* / Q000201:enzymology; D011108:Polymers; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000276:immunology; D000071681:Tartrate-Resistant Acid Phosphatase
D005404:Fixatives; D007106:Immune Sera; D007375:Interleukin-1; D007527:Isoenzymes; D008070:Lipopolysaccharides; D011108:Polymers; D011994:Recombinant Proteins; D005557:Formaldehyde; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D015232:Dinoprostone; D007213:Indomethacin; C003043:paraform