Purification of lipases, phospholipases and kinases by heparin-Sepharose chromatography.
Heparin interacts with lipases, phospholipases and kinases. Immobilized heparin can be used for the purification of diacylglycerol and triacylglycerol lipases, phospholipases A2 and C and protein and lipid kinases. The use of heparin-Sepharose is an important development in analytical and preparative techniques for the separation and isolation of lipases, phospholipases and kinases.
Farooqui
A A
AA
Department of Medical Biochemistry, Ohio State University, Columbus 43210.
Yang
H C
HC
Horrocks
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LA
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006493:Heparin; D006801:Humans; D008049:Lipase / Q000302:isolation & purification*; D010740:Phospholipases / Q000302:isolation & purification*; D010770:Phosphotransferases / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
C037405:heparin-sepharose; D006493:Heparin; D012685:Sepharose; D010770:Phosphotransferases; D010740:Phospholipases; D008049:Lipase