Striatal G-proteins: effects of unilateral 6-hydroxydopamine lesions.
To investigate the molecular mechanisms of denervation-induced dopamine receptor supersensitivity, levels of striatal G-proteins were examined in 6-hydroxydopamine (6-OHDA)-lesioned rats. Increased levels of the alpha-subunits of Gs and G(olf), as measured by immunoblotting, were observed 8 days postlesion and remained elevated up to day 28 (25-45% over the time course). G(i) levels were significantly increased only on day 8 (20%) and G(o) levels remained unaltered. These results suggest that stimulatory G-proteins may be involved in the long-term maintenance of dopamine receptor supersensitivity following denervation.
Marcotte
E R
ER
Department of Psychiatry, McMaster University, Hamilton, Ont., Canada.
Sullivan
R M
RM
Mishra
R K
RK
D000818:Animals; D019204:GTP-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism*; D016627:Oxidopamine / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000187:drug effects
D017447:Receptors, Dopamine D1; D016627:Oxidopamine; D019204:GTP-Binding Proteins