Sequencing of oligonucleotide phosphorothioates based on solid-supported desulfurization.
We described a solid-supported desulfurization procedure allowing easy access to the sequence analysis of oligonucleotide phosphorothioates. The described method is based upon selective removal of the 2-cyanoethyl phosphate protecting groups, followed by iodine-promoted desulfurization of the resulting phosphorothioate diesters. Automatic oxidation of oligonucleotide phosphorothioates, anchored via an ester linkage to a standard solid support (LCAA/CPG), is combined with Maxam-Gilbert solid-support sequencing. The overall procedure allows rapid simultaneous sequence analysis of several oligonucleotide analogs.
Wyrzykiewicz
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ISIS Pharmaceuticals, Carlsbad Research Center, CA 92008.
Cole
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D001483:Base Sequence; D004247:DNA; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*; D063086:Organothiophosphates / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*; D013455:Sulfur / Q000737:chemistry; D013873:Thionucleotides / Q000737:chemistry*
D009841:Oligonucleotides; D063086:Organothiophosphates; D013873:Thionucleotides; D013455:Sulfur; D004247:DNA