Expression of creatine kinase M and B mRNAs in treadmill trained rat skeletal muscle.
Gastrocnemius muscle from treadmill trained rats was analyzed for creatine kinase (CK) isoenzyme activities by agarose electrophoresis and M and B CK mRNA levels by Northern blot analysis. Total CK activity in exercise-trained (143 (SD 15) U/g) and control (154 (SD 16) U/g) muscles did not differ. CK-MB increased 220% in exercised-trained muscle compared to controls. CK-B subunit mRNA increased 40%, while CK-M subunit mRNA decreased 42% in exercised-trained muscle compared to control. Thus, gene expression of CK isoenzymes appears to be partially controlled at the level of transcription following exercise training.
Apple
F S
FS
Department of Laboratory Medicine and Pathology, Hennepin County Medical Center, University of Minnesota School of Medicine, Minneapolis 55415.
Billadello
J J
JJ
D000818:Animals; D003402:Creatine Kinase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics; D009132:Muscles / Q000378:metabolism*; D005082:Physical Exertion; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D007527:Isoenzymes; D012333:RNA, Messenger; D003402:Creatine Kinase