Determination of L-2-oxothiazolidine-4-carboxylic acid (Procysteine) in human plasma by high-performance liquid chromatography.
A new high-performance liquid chromatographic (HPLC) method has been developed for the determination of the cysteine prodrug, L-2-oxothiazolidine-4-carboxylic acid (Procysteine), in human plasma. Samples were mixed with 5% metaphosphoric acid and the supernatants chromatographed using a reversed-phase analytical column. Procysteine was detected spectrophotometrically at 230 nm and quantitated by comparison with a standard curve of known amounts of Procysteine (20-1000 microM) in plasma. The coefficients of variation for 48.6 microM and 676 microM control pools were 6.5 and 4.0% respectively (n = 46). Deviations from the expected concentrations were less than 2%. The method has been utilized to evaluate the pharmacokinetics of a wide range of oral and intravenous doses.
Webb
L E
LE
Baxter Healthcare Corporation, Round Lake, IL 60073.
Gechman
M
M
Story
K
K
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D015658:HIV Infections / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D011761:Pyrrolidonecarboxylic Acid; D011786:Quality Control; D012015:Reference Standards; D012128:Respiratory Distress Syndrome / Q000097:blood; D013056:Spectrophotometry, Ultraviolet; D013844:Thiazoles / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D053778:Thiazolidines
D007202:Indicators and Reagents; D013844:Thiazoles; D053778:Thiazolidines; D011761:Pyrrolidonecarboxylic Acid; C029536:2-oxothiazolidine-4-carboxylic acid