title: Demonstration of a fluoride dose response with an in situ single-section dental caries model.
abstract: The in situ dental caries model developed in Glasgow was evaluated by determining whether it could demonstrate a fluoride dose response in a crossover study using 0, 250, and 1,000 ppm F from sodium fluoride dentifrices. In situ appliances, each carrying four enamel sections with multiple artificial carious lesions, were worn by 12 dentate subjects for three 5-week experimental runs, during which they brushed twice daily with a test paste. Mineral changes in the lesions were measured by analyzing microradiographs, using an image analysis system, after 0, 2, 4, and 5 weeks and the rate of change calculated. Two-way analysis of variance showed no significant intersubject effects (for any of the parameters), but demonstrated strong evidence of a linear fluoride dose effect: total mineral loss (delta z) p = 0.0596; lesion body p = 0.0423; surface zone p = 0.0081. However, the exclusion of the data from 1 low-caries (DMFS = 1), high-calculus-forming subject produced even stronger evidence as to the efficacy of this model (delta z p = 0.0226; lesion body p = 0.0164; surface zone p = 0.0081). Hence, these results suggest this model is useful for studying fluoride products according to guidelines of American Dental Association.
authors.affiliation: Department of Adult Dental Care, Glasgow Dental Hospital and School, UK.
authors.forename: F A
authors.initials: FA
authors.lastname: Damato
authors.forename: K W
authors.initials: KW
authors.lastname: Stephen
mesh_terms: D000328:Adult; D000571:American Dental Association; D000704:Analysis of Variance; D015169:Colony Count, Microbial; D004245:DMF Index; D003731:Dental Caries / Q000188:drug therapy*; D003773:Dental Plaque / Q000382:microbiology; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D004341:Drug Evaluation / Q000379:methods; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007778:Lactobacillus / Q000302:isolation & purification; D008297:Male; D012044:Regression Analysis; D012969:Sodium Fluoride / Q000008:administration & dosage* / Q000627:therapeutic use; D013295:Streptococcus mutans / Q000302:isolation & purification; D014089:Tooth Remineralization / Q000379:methods*; D014100:Toothpastes / Q000627:therapeutic use*; D014481:United States
chemical_list: D014100:Toothpastes; D012969:Sodium Fluoride