High EDTA concentrations cause entrapment of small DNA molecules in the compression zone of pulsed field gels, resulting in smaller than expected insert sizes in YACs prepared from size selected DNA.
Funke
R P
RP
Center for Legume Research, University of Tennessee, Knoxville 37901-1071.
Kolchinsky
A
A
Gresshoff
P M
PM
D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004492:Edetic Acid; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D013025:Glycine max / Q000235:genetics*; D014170:Transformation, Genetic
D004247:DNA; D004492:Edetic Acid