Characterization of the sat4 gene encoding a streptothricin acetyltransferase in Campylobacter coli BE/G4.
The sat4 streptothricin resistance gene from Campylobacter coli BE/G4 was cloned into pUC18, and its nucleotide sequence was determined. Streptothricin acetyltransferase activity was detected in Escherichia coli cells containing recombinant plasmid pAT132 which carries the sat4 gene as an insert. The deduced amino acid sequence displayed 21-27% amino acid identity with streptothricin acetyltransferases from E. coli and streptothricin producers Streptomyces lavendulae and Streptomyces noursei. The sat4 gene was detected by hybridization in clinical and environmental isolates of Campylobacter spp.
Jacob
J
J
Unité des Agents Antibactériens, CNRS, Institut Pasteur, Paris, France.
Evers
S
S
Bischoff
K
K
Carlier
C
C
Courvalin
P
P
D000123:Acetyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D017000:Campylobacter coli / Q000201:enzymology* / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013309:Streptothricins
D013309:Streptothricins; D000123:Acetyltransferases; C044496:streptothricin acetyltransferase