title: Early phenotypic choices by neuronal precursors, revealed by clonal analysis of the chick embryo hindbrain.
abstract: The mechanisms that generate diverse neuronal phenotypes within the central nervous system are thought to involve local cues or cell-cell interactions acting late in neurogenesis, perhaps as late as the last precursor cell division. We describe here a clonal analysis of neuronal development in the chick hindbrain, using an intracellular tracer to mark single precursor cells, that suggests the operation of an alternative strategy. The majority of clones, ranging from 1 to 46 cells, contained neurons of only one of several possible phenotypes. These single-phenotype clones were not positionally restricted within a rhombomere but were interspersed with other clones containing distinct phenotypes. The assignment of neuronal phenotype in this brain region may, therefore, be made in early precursors and remembered through several rounds of mitotic expansion and dispersal.
authors.affiliation: MRC Brain Development Programme, Division of Anatomy and Cell Biology, United Medical and Dental Schools, Guy's Hospital, London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Lumsden
authors.forename: J D
authors.initials: JD
authors.lastname: Clarke
authors.forename: R
authors.initials: R
authors.lastname: Keynes
authors.forename: S
authors.initials: S
authors.lastname: Fraser
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002642:Chick Embryo; D002999:Clone Cells; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D012249:Rhombencephalon / Q000166:cytology / Q000196:embryology*; D013234:Stem Cells / Q000502:physiology*