title: [Electron microscopic study of a macroporous calcium phosphate ceramic implanted in an osseous site].
abstract: Cellular and tissular responses to intraosseous graft of a macroporous calcium phosphate ceramic was studied using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Twelve specimens were implanted in 6 rabbits (tibiae), taken at day 14 after implantation and processed either for TEM (6 samples) or SEM (6 samples). As early as day 14 after implantation osteogenesis so that resorption of the newly formed bone and of the biomaterial, were observed at the surface of the ceramic, inside the macropores. Osteoblasts were clearly visible and well differentiated with abundant rough endoplasmic reticulum and large Golgi zone. The resorption processes were associated with 2 types of multinucleated cells. Based on ultrastructural observations (cellular characteristics and measurement of the microporosity) it appears that incompletely differentiated osteoclast was the major cell responsible of the biodegradation of the ceramic. These results suggest that the cellular events occurring at the surface of a macroporous calcium phosphate ceramic are similar to that observed in physiological bone remodelings.
authors.affiliation: Laboratoire d'Histologie-Embryologie, Faculté de Médecine, ANGERS.
authors.forename: F
authors.initials: F
authors.lastname: Grizon
authors.forename: R
authors.initials: R
authors.lastname: Filmon
authors.forename: D
authors.initials: D
authors.lastname: Chappard
authors.forename: A
authors.initials: A
authors.lastname: Rebel
authors.forename: M F
authors.initials: MF
authors.lastname: Basle
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000601:surgery / Q000648:ultrastructure*; D002130:Calcium Phosphates; D002516:Ceramics; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D016062:Porosity; D019736:Prostheses and Implants; D011817:Rabbits
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous
vernacular_title: Etude en microscopie électronique d'une céramique phosphocalcique macroporeuse implantée en site osseux.