The human ubiquitin-conjugating enzyme UbcH1 is involved in the repair of UV-damaged, alkylated and cross-linked DNA.
The human ubiquitin-conjugating enzyme UbcH1 shows 69% identity to the Saccharomyces cerevisiae RAD6/UBC2 which plays a key role in DNA repair. To examine the function of UbcH1 (formerly named E2, M(r) 17,000), [(1990) EMBO J. 9, 1431-1435]) we tested its ability to functionally substitute for yeast RAD6/UBC2 in the recovery of cells from various DNA damage. Complementation by expression of the human UbcH1 cDNA revealed that the UbcH1 carries out the function of S. cerevisiae RAD6/UBC2 in the repair of UV-damaged, alkylated and cross-linked DNA.
Kaiser
P
P
Institute of Biochemistry, University of Innsbruck, Austria.
Mansour
H A
HA
Greeten
T
T
Auer
B
B
Schweiger
M
M
Schneider
R
R
D000478:Alkylation; D003432:Cross-Linking Reagents; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D006801:Humans; D008025:Ligases / Q000502:physiology*; D008730:Methoxsalen / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D044763:Ubiquitin-Conjugating Enzymes; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; C066752:UBC1 protein, S cerevisiae; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases; D008730:Methoxsalen