title: The conversion of ethanolamine and of its metabolites to choline in human neuroblastoma clones: effect of differentiation induced by retinoic acid.
abstract: The sequential methylation of ethanolamine (Etn) or phosphorylethanolamine to the corresponding choline (Cho) derivatives was studied in both undifferentiated and retinoic acid (RA) differentiated human neuroblastoma clones LA-N-1 and LA-N-2. Conversion of Etn derivatives to the respective Cho metabolites was low in both cell types. However, after treatment of the cultures with ethanol or RA, the methylation of phosphoryl-Etn was stimulated while that of phosphatidyl-Etn was severely reduced in both cholinergic LA-N-2 and catecholaminergic LA-N-1 cells.
authors.affiliation: Centre de Neurochimie du CNRS, Strasbourg, France.
authors.forename: N E
authors.initials: NE
authors.lastname: Haidar
authors.forename: C
authors.initials: C
authors.lastname: Andriamampandry
authors.forename: M
authors.initials: M
authors.lastname: Carrara
authors.forename: J N
authors.initials: JN
authors.lastname: Kanfer
authors.forename: L
authors.initials: L
authors.lastname: Freysz
authors.forename: H
authors.initials: H
authors.lastname: Dreyfus
authors.forename: R
authors.initials: R
authors.lastname: Massarelli
mesh_terms: D001692:Biological Transport; D002454:Cell Differentiation / Q000187:drug effects; D002794:Choline / Q000378:metabolism*; D002999:Clone Cells; D000431:Ethanol / Q000494:pharmacology; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009447:Neuroblastoma / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D004983:Ethanolamines; D000431:Ethanol; D014212:Tretinoin; D019856:Ethanolamine; D002794:Choline