[Study of human lymphocyte proteins using two-dimensional electrophoresis].
Using O'Farrell 2DE modified method, a panel of human lymphocyte proteins have been studied. A 2-D map involving 273 fractions characterized in M(r)/pI coordinates has been developed. Some proteins were identified on the map and some comparability of experimental results to the Celis et al. data demonstrated. Ten protein fractions displaying some variability in a sample of 30 patients were revealed. A change in the amount of the E2 fraction M(r)/pI -51.0/5.70 was found among patients with chromosome 5 short arm deletion and translocation.
Lukashova
I V
IV
Kovalev
L I
LI
Efimochkin
A S
AS
Shishkin
S S
SS
D001798:Blood Proteins / Q000235:genetics* / Q000302:isolation & purification; D002872:Chromosome Deletion; D002895:Chromosomes, Human, Pair 5; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D011110:Polymorphism, Genetic
D001798:Blood Proteins
Izuchenie belkov limfotsitov cheloveka s pomoshch'iu dvumernogo élektroforeza.