title: Recombinant secreted nonpancreatic phospholipase A2 induces a synovitis-like inflammation in the rat air pouch.
abstract: OBJECTIVE
  The aim of this study was to test the proinflammatory action of human secreted phospholipase A2 (sPLA2) in an animal model of synovitis-like inflammation and to compare it with a Group 1 (porcine pancreatic) and a Group 2 (Naja mocambique mocambique) PLA2.
  
  
  METHODS
  The subcutaneous injection of air in the rat pouch induced the formation of a connective tissue cavity lined with cells closely resembling a synovium. sPLA2 (0.01-10 micrograms/pouch) porcine pancreatic (10 micrograms/pouch), PLA2 or Naja mocambique mocambique PLA2 (10 micrograms/pouch) were injected in a 6-day rat air pouch.
  
  
  RESULTS
  Our data show that recombinant human enzyme PLA2 (0.01-10 micrograms/pouch), but not Naja mocambique mocambique (Group 2 PLA2) and porcine pancreatic PLA2 (Group 1 PLA2), was able to exacerbate the inflammatory response without increasing eicosanoid release.
  
  
  CONCLUSIONS
  Only sPLA2 was able to exacerbate rat air pouch and its effect was unrelated to eicosanoid release.
authors.affiliation: Department of Experimental Pharmacology, University of Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Cirino
authors.forename: C
authors.initials: C
authors.lastname: Cicala
authors.forename: L
authors.initials: L
authors.lastname: Sorrentino
authors.forename: F M
authors.initials: FM
authors.lastname: Maiello
authors.forename: J L
authors.initials: JL
authors.lastname: Browning
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015777:Eicosanoids / Q000032:analysis; D007958:Leukocyte Count / Q000187:drug effects; D008297:Male; D010741:Phospholipases A / Q000009:adverse effects*; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000009:adverse effects; D013585:Synovitis / Q000097:blood / Q000139:chemically induced* / Q000473:pathology
chemical_list: D015777:Eicosanoids; D011994:Recombinant Proteins; D010741:Phospholipases A; D054467:Phospholipases A2