[The use of a primary astrocyte culture for modelling HIV neurological infection].
The article deals with the results of the experiment substantiating the in vitro model of HIV neuro-infection. In this work primary glial (astrocytic) tissue cultures obtained from normal human and animal (guinea pig) brain tissue were used. As revealed in this investigation, the following phenomena could be observed in human brain tissue monolayer culture, infected with HIV and subsequently subcultured: (a) the stimulation of tissue-cell growth; (b) the formation of multinuclear glial cells; (c) the presence of virus-specific proteins in astrocyte cytoplasm, detected by immunofluorescent and electrophoretic techniques; (d) the presence of HIV-1 DNA provirus in infected astrocytes. Cyto-destruction was not observed, reverse transcriptase activity was absent.
Rytik
P G
PG
Kvacheva
Z B
ZB
Eremin
V F
VF
Poleshchuk
N N
NN
Popov
S A
SA
Müller
W B
WB
Asher
D M
DM
D015526:AIDS Dementia Complex / Q000382:microbiology* / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000382:microbiology* / Q000648:ultrastructure; D002460:Cell Line; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006168:Guinea Pigs; D015497:HIV-1; D006801:Humans; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast
Ispol'zovanie pervichnoĭ kul'tury astrotsitov dlia modelirovaniia VICh-neĭroinfektsii.