[The biochemical and immunochemical characteristics of the protein preparations obtained by different methods from a biomass of Neisseria meningitidis serogroup B].
Protein preparations containing group B N. meningitidis outer membrane peptides of classes 1 (44-47 kD), 2 (40-42 kD) and 5 (26-30 kD) have been obtained from the biomass of group B N. meningitidis, grown in a fermenter in a synthetic medium under the conditions of controlled cultivation with regulated oxygen content and the intermittent drainage and filling of the fermenter and with the use of different extraction methods (lithium chloride extraction by the method of C. E. Frasch et al.; sodium deoxycholate and potassium thiocyanate extraction). The chemical composition of these preparations and their peptide content have been shown to depend on the method of extraction; the preparation containing the least amount of contaminating substances is obtained by extraction with potassium thiocyanate. High-molecular fractions of these protein preparations, studied by the method of immunoblotting, contain antigens cross-reacting with meningococcal sera, groups A and C.
Karabak
V I
VI
Basnak'ian
I A
IA
Aleksakhina
N N
NN
D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D001431:Bacteriological Techniques; D003470:Culture Media; D007120:Immunochemistry; D008970:Molecular Weight; D009345:Neisseria meningitidis / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D012703:Serotyping
D001426:Bacterial Proteins; D003470:Culture Media
Biokhimicheskaia i immunokhimicheskaia kharakteristika belkovykh preparatov, poluchennykh iz biomassy Neisseria meningitidis serogruppy B.