alpha-Tocopherol protects against radical-induced injury in cultured neurons.
The effect of alpha-tocopherol on neurons in protecting against radical-induced injuries caused by cumene hydroperoxide was assessed using a culture system. Structural and biochemical deteriorations in both clonal neuroblastoma cells and primary cultured neurons were induced by cumene hydroperoxide. These deteriorative changes were prevented prevented by pretreatment of alpha-tocopherol. Since simultaneous supplementation of alpha-tocopherol and cumene hydroperoxide had limited protective effects, prophylactic administration of alpha-tocopherol for risk patients with brain diseases concerned with damages induced by free radical is recommended to hamper the progression of neuronal impairment after attacks.
Amano
S
S
Department of Pathology, Shiga University of Medical Science, Otsu, Japan.
Ohashi
M
M
Kirihara
M
M
Yang
X H
XH
Hazama
F
F
D000818:Animals; D001555:Benzene Derivatives / Q000633:toxicity; D002478:Cells, Cultured; D002999:Clone Cells; D005609:Free Radicals / Q000009:adverse effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000494:pharmacology*
D001555:Benzene Derivatives; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D007770:L-Lactate Dehydrogenase; C007164:cumene hydroperoxide