Fungal metabolites. X. The effect of peptide antibiotics, trichosporin-Bs, on the respiratory activity of mitochondria.
The effect of the trichosporin-Bs, peptide antibiotics, on the respiration of mitochondria was investigated. Trichosporin-Bs stimulated the respiratory rate of state 4 rat liver mitochondria in a dose-dependent manner. The maximum respiratory rate obtained by trichosporin-Bs was essentially the same as for 2,4-dinitrophenol and SF6847. Trichosporin-B-VIb released oligomycin-inhibited respiration of mitochondria. This means that trichosporin-Bs are uncouplers of oxidative phosphorylation. The stimulatory effect of trichosporin-B-VIb, a component of trichosporin-Bs, on mitochondrial respiration was increased by inorganic phosphate but not by other permeant anions studied. These results suggest that the stimulation of mitochondrial respiration by trichosporin-B-VIb is mediated by the same mechanism as is operating in the case of hypelcins and alamethicins. Furthermore, the relative potencies of trichosporin-Bs on the mitochondrial respiration and their relative hydrophobicities were examined. A clear relationship was observed between the uncoupling potencies of trichosporin-Bs and their relative hydrophobicities.
Okuda
M
M
Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Iida
A
A
Uesato
S
S
Nagaoka
Y
Y
Fujita
T
T
Takaishi
Y
Y
Terada
H
H
D019297:2,4-Dinitrophenol; D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002458:Cell Fractionation; D004140:Dinitrophenols / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption / Q000187:drug effects*; D010455:Peptides; D010710:Phosphates / Q000494:pharmacology; D051381:Rats; D014475:Uncoupling Agents / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004140:Dinitrophenols; D009570:Nitriles; D009840:Oligomycins; D010455:Peptides; D010710:Phosphates; D014475:Uncoupling Agents; C001784:SF 6847; D019297:2,4-Dinitrophenol