[Comparative study of chemically modified hemoglobins as a basis for oxygen-carrying blood substitutes].
The series of hemoglobin derivatives were obtained with the different content of the polymer fractions; their comparative study by the methods of gel-chromatography and SDS-electrophoresis was conducted and the estimation of their functional, rheological, colloid-oncotic properties and the degree of immunogenicity was carried out in order to choose the optimal substance judging by their physico-chemical, gas-carrying and antigenic characteristics. It was shown that the polymerised hemoglobin with the polymer content of 25-30% has the optimal properties as the oxygen carrier. The 10% solution of this compound possesses oxygen-carrying characteristics identical to donors erythrocytes, viscosity of no more than 1.5 sP, colloid oncotic pressure in the range of 15-17.5 torr, it practically doesn't influence the aggregation of the formula blood elements and has low antigenicity. The results obtained allow to consider this type of poly-hemoglobin as a potential oxygen-carrying blood substitute.
Azhigirova
M A
MA
Viazova
E P
EP
Shuvalova
A L
AL
Vashkevich
M G
MG
D001692:Biological Transport / Q000502:physiology; D001704:Biopolymers; D001802:Blood Substitutes / Q000737:chemistry*; D002850:Chromatography, Gel; D003102:Colloids; D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins / Q000737:chemistry*; D010100:Oxygen / Q000097:blood*; D011732:Pyridoxal Phosphate / Q000097:blood / Q000276:immunology; D013223:Statistics as Topic; D014783:Viscosity
D001704:Biopolymers; D001802:Blood Substitutes; D003102:Colloids; D006454:Hemoglobins; D011732:Pyridoxal Phosphate; D010100:Oxygen
Sravnitel'noe issledovanie khimicheski modifitsirovannykh gemoglobinov v kachestve osnovy krovezamenitelia--perenoschika kisloroda.