[Cloning the human cytomegalovirus immediate-early gene and expression of it in Escherichia coli].
The major immediate early gene of human cytomegalovirus has been cloned. The fragment of the immediate early protein containing 351 amino acids has been fused with cro-beta-galactosidase and expressed in Escherichia coli cells. The obtained recombinant protein reacts in immunoblotting with the antibodies in human sera from patients suffering from acute cytomegalovirus infection.
Klichko
V I
VI
Zvonarev
A Iu
AIu
Shatalin
K Iu
KIu
Kuliakina
M N
MN
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics*; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics*; D017781:Genes, Immediate-Early; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D012097:Repressor Proteins / Q000235:genetics; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D001616:beta-Galactosidase / Q000235:genetics
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D001616:beta-Galactosidase
Klonirovanie gena osnovnogo predrannego belka tsitomegalovirusa cheloveka i ékspressiia ego v kletkakh Escherichia coli.