Increased activation of 2',3'-dideoxycytidine by acivicin.
The effect of acivicin (ACV) on cell growth, cell cycle progression, and intracellular accumulation of 2',3'-dideoxycytidine (ddC) was examined. Treatment of H9 cells with ACV (1 microM) plus ddC (25 microM) for 24 hrs inhibited cell growth to 74 +/- 7%, arrested cells in the S phase, and increased the cellular ddC accumulation to 195-208% of control. The concentrations of ddCTP, the active form of ddC, was increased 3-4 fold in ACV treated cells.
Agarwal
R P
RP
Department of Medical Oncology, University of Miami School of Medicine, FL 33136.
D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004357:Drug Synergism; D006801:Humans; D007555:Isoxazoles / Q000494:pharmacology*; D016047:Zalcitabine / Q000378:metabolism* / Q000494:pharmacology
D007555:Isoxazoles; D016047:Zalcitabine; C006303:acivicin