title: Capillary zone electrophoretic determination of histamine in fish.
abstract: Histamine, the principal causative agent in scombroid food poisoning, was analyzed in seafood by a new, rapid, and sensitive method using capillary zone electrophoresis (CZE) with UV detection at 210 nm. Incurred histamine in methanolic fish extracts migrated within 4 min in a fused silica capillary filled with 0.02 M citrate buffer, pH 2.5, under an applied potential of 375 V/cm. The analytical response was linear from 0.5 to 100 ppm histamine (correlation coefficient, r = 0.999). The coefficients of variation for migration time and peak area response were < 1 and < 3%, respectively. Recovery of histamine in fortified fish composites was satisfactory. CZE was considered for alternative application in seafood speciation.
authors.affiliation: U.S. Food and Drug Administration, Northeast Regional Laboratory, Brooklyn, NY 11232-1593.
authors.forename: B
authors.initials: B
authors.lastname: Mopper
authors.forename: C J
authors.initials: CJ
authors.lastname: Sciacchitano
mesh_terms: D000818:Animals; D004586:Electrophoresis / Q000379:methods*; D006632:Histamine / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D012680:Sensitivity and Specificity; D014413:Tuna
chemical_list: D006632:Histamine