Simultaneous determination of organic acids and vitamin C in green beans by liquid chromatography.
A method is described for determining and quantitating organic acids (oxalic, malic, citric, and fumaric) and vitamin C by liquid chromatography with a UV-visible detector that allows simultaneous monitoring at 2 wavelengths. The method was applied to samples of green beans (Phaseolus vulgaris L.). Recoveries were 97.8% for oxalic acid, 98.9% for malic acid, 98.7% for citric acid, 99.2% for fumaric acid, and 98.5% for vitamin C. Method precisions (coefficients of variation) were 1.7% for oxalic acid, 0.8% for malic acid, 0.9% for citric acid, 1.5% for fumaric acid, and 1.2% for vitamin C. Measurement precisions (coefficients of variation) were 1.32% for oxalic acid, 0.33% for malic acid, 0.62% for citric acid, 1.01% for fumaric acid, and 0.39% for vitamin C. Limits of detection were 0.025 mg/mL for oxalic acid, 0.022 mg/mL for malic acid, 0.024 mg/mL for citric acid, 1.0 x 10(-4) mg/mL for fumaric acid, and 2.7 x 10(-4) mg/mL for vitamin C.
Vazquez Oderiz
M L
ML
Universidad de Santiago de Compostela, Facultad de Farmacia, Departmento de Química Analítica, Nutrición y Bromatología, La Coruña, Spain.
Vazquez Blanco
M E
ME
Lopez Hernandez
J
J
Simal Lozano
J
J
Romero Rodriguez
M A
MA
D000143:Acids / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D002951:Citrates / Q000032:analysis; D019343:Citric Acid; D007887:Fabaceae / Q000737:chemistry*; D005650:Fumarates / Q000032:analysis; D008293:Malates / Q000032:analysis; D010070:Oxalates / Q000032:analysis; D019815:Oxalic Acid; D010946:Plants, Medicinal; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000143:Acids; D002951:Citrates; D005650:Fumarates; D008293:Malates; D010070:Oxalates; D019343:Citric Acid; C030298:malic acid; C032005:fumaric acid; D019815:Oxalic Acid; D001205:Ascorbic Acid