Stimulation of tyrosine- and serine-phosphorylation of focal adhesion kinase in mouse 3T3 cells by fibronectin and fibroblast growth factor.
Phosphorylation of both tyrosine and serine residues of focal adhesion kinase (FAK) was stimulated by the adhesion of BALB/c mouse 3T3 cells to fibronectin, but phosphorylation of threonine was not detectable. Acidic and basic fibroblast growth factors also stimulated the phosphorylation of serine and tyrosine of FAK in cells adhered to poly-L-lysine, but epidermal growth factor and platelet-derived growth factor did not. A fusion protein of fibronectin and basic fibroblast growth factor effectively induced the phosphorylation of FAK. Phosphorylation of FAK in the rat myoblast L-6 cell line, which lacks fibroblast growth factor receptors, was not stimulated by fibroblast growth factors, suggesting that the interaction of fibroblast growth factors with their receptors might cause the phosphorylation of FAK.
Hatai
M
M
Biology Division, National Cancer Center Research Institute, Tokyo, Japan.
Hashi
H
H
Mogi
A
A
Soga
H
H
Yokota
J
J
Yaoi
Y
Y
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology*; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010766:Phosphorylation / Q000187:drug effects; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D051381:Rats; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism; D012694:Serine / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D003432:Cross-Linking Reagents; D005353:Fibronectins; D017468:Receptors, Fibroblast Growth Factor; D014443:Tyrosine; D012694:Serine; D005346:Fibroblast Growth Factors; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496050:PTK2 protein, human; C496051:Ptk2 protein, mouse; C496052:Ptk2 protein, rat