[Molecular cytogenetic approach on malignant lymphoma].
Diagnosis of malignant lymphoma is often complicated by difficulties in distinguishing histologically from reactive or benign lymphoadenopathy. The clonality of the lymphoma cells by a cytogenetic analysis can provide a useful criterion for the diagnosis of lymphoma. Cytogenic studies on lymphoma have shown many chromosome translocations and deletions. Molecular analysis revealed the Ig gene or T cell receptor gene rearrangements in DNAs from lymphoma. In t(14;18) cases, it was amplified as a translocation-specific fragment by PCR. These findings indicated that chromosome and gene rearrangement analysis may be useful for lymphoma cells, especially for making a clinical diagnosis.
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Department of Clinical Laboratory and Medicine, Tohoku University School of Medicine, Sendai.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002869:Chromosome Aberrations; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D007621:Karyotyping; D008223:Lymphoma / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic