Genetically engineered synthesis of natural products.
The feasibility of multi-step, one-flask total synthesis of natural products is demonstrated by cloning and overexpression of the corresponding biosynthetic enzymes from plant and microbial sources. As many as eight or nine enzymes can be recombined in vitro to reach an advanced intermediate in the vitamin B12 pathway.
Scott
A I
AI
Department of Chemistry, Texas A&M University, College Station 77843-3255.
D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004798:Enzymes / Q000096:biosynthesis*; D005818:Genetic Engineering; D008969:Molecular Sequence Data; D010944:Plants / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis
D004798:Enzymes; D011994:Recombinant Proteins