title: Chondrocyte-fibrin matrix transplants for resurfacing extensive articular cartilage defects.
abstract: Cartilage resurfacing by chondrocyte implantation, with fibrin used as a vehicle, was examined in large (12 mm) full-thickness articular cartilage defects in horses. Articular chondrocytes, isolated from a 9-day-old foal, were mixed with fibrinogen and injected with thrombin, in a 1:1 mixture, into 12 mm circular defects on the lateral trochlea of the distal femur of eight normal horses. The contralateral femoropatellar (knee) joint served as a control in which the defect was left empty. Synovial fluid from the femoropatellar joints was sampled on days 0, 4, 7, 30, 120, and 240 postoperatively. Groups of four horses were killed at 4 or 8 months postoperatively, and the repair tissue was evaluated by gross and histologic examination with use of hematoxylin and eosin and safranin O staining and by autoradiography. Biochemical analyses included quantitation of proteoglycan, total collagen, and type-II collagen in the repair tissue. Grossly, grafted defects had improved filling of the cartilage lesions; histologically, these areas consisted of differentiated chondrocytes in the deep and middle zones. The cellular arrangement in these zones resembled that of hyaline cartilage. The control defects contained poorly attached fibrous tissue throughout. Grafted tissue at 8 months had increased proteoglycan synthesis evident by both safranin O staining and autoradiography. Glycosaminoglycan quantitation by dye-binding assay confirmed a significantly elevated glycosaminoglycan content in grafted defects (58.8 micrograms/mg of dry weight) compared with control defects (27.4 micrograms/mg; p < 0.05). Similarly, the levels of chondroitin sulfate/dermatan sulfate was significantly elevated in the grafted defects, and this was the predominant glycosaminoglycan epitope present. There was a statistically significant (p < 0.05) increase in type-II collagen in the grafted tissue at 8 months (61.2% grafted; 25.1% control). This resurfacing attempt with use of allograft chondrocytes, secured in large full-thickness articular defects with polymerized fibrin, resulted in an improved cartilage surface in comparison with the control defects, a significantly greater aggrecan level, and a significantly higher proportion of type-II collagen.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
authors.forename: D A
authors.initials: DA
authors.lastname: Hendrickson
authors.forename: A J
authors.initials: AJ
authors.lastname: Nixon
authors.forename: D A
authors.initials: DA
authors.lastname: Grande
authors.forename: R J
authors.initials: RJ
authors.lastname: Todhunter
authors.forename: R M
authors.initials: RM
authors.lastname: Minor
authors.forename: H
authors.initials: H
authors.lastname: Erb
authors.forename: G
authors.initials: G
authors.lastname: Lust
mesh_terms: D000818:Animals; D001345:Autoradiography; D001857:Bone Matrix / Q000737:chemistry / Q000378:metabolism / Q000637:transplantation; D002357:Cartilage Diseases / Q000601:surgery; D002358:Cartilage, Articular / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation; D002469:Cell Separation; D017690:Cell Transplantation; D002809:Chondroitin Sulfates / Q000032:analysis / Q000378:metabolism; D003094:Collagen / Q000032:analysis / Q000378:metabolism; D005260:Female; D005337:Fibrin / Q000032:analysis* / Q000378:metabolism; D006736:Horses; D007719:Knee Joint; D008297:Male; D014184:Transplantation, Homologous
chemical_list: D005337:Fibrin; D002809:Chondroitin Sulfates; D003094:Collagen