Phaseolus vulgaris lectin labelled and GABA immunogold stained terminals in nucleus rotundus. An EM study.
Having injected Phaseolus Vulgaris Lectin (PhA-L) iontophoretically to subpretectal (SP) and postero-ventralis (PV) thalamic nuclei, labelled fibers and terminals appeared in nucleus rotundus (Rt). The PhA-L immunostained fibers are divided dichotomically and they develop long terminal sections with large varicosities. In EM preparates PhA-L labelled terminals were observed establishing axo-somatic, axo-dendritic symmetrical synapses. These terminals were stained by GABA immunogold procedure and proved to be GABA-ergic, i.e. the neurons of SP and PV nuclei supply the principal neurons of Rt with GABA positive terminals, which are located on specific, exponent loci of the principal neuron.
Tömböl
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T
1st Department of Anatomy, Semmelweis University, Medical School, Budapest, Hungary.
Egedi
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G
Németh
A
A
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002645:Chickens; D007150:Immunohistochemistry; D007478:Iontophoresis; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000648:ultrastructure*; D010835:Phytohemagglutinins; D013787:Thalamic Nuclei / Q000648:ultrastructure*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D010835:Phytohemagglutinins; D005680:gamma-Aminobutyric Acid