A simple and rapid fluorometric determination method of alpha 1-acid glycoprotein in serum using quinaldine red.
We examined different fluorescent probes suitable for fluorometric determination of alpha 1-acid glycoprotein (AGP) in serum. Quinaldine red (QR) was shown to bind strongly and selectively to AGP. Taking advantage of the enhanced fluorescence of QR in the presence of AGP, we developed a direct method for the determination of serum AGP without removal of other serum proteins such as albumin. AGP concentrations in serum of healthy volunteers and patients correlated well with results from the conventional single radial immunodiffusion (SRID) method (r = 0.93, slope = 1). The newly developed method is faster and has a larger analytical concentration range than the SRID method. This method can also be used to determine AGP in serum of experimental animals, and it can serve to monitor AGP serum concentrations for pharmacokinetic evaluation of basic drugs.
Imamura
H
H
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Maruyama
T
T
Okabe
H
H
Shimada
H
H
Otagiri
M
M
D000328:Adult; D000735:Androstenedione / Q000737:chemistry; D001798:Blood Proteins / Q000378:metabolism; D003956:Dialysis; D005456:Fluorescent Dyes; D006801:Humans; D008875:Middle Aged; D009961:Orosomucoid / Q000032:analysis*; D011485:Protein Binding; D011798:Quinaldines; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D001798:Blood Proteins; D005456:Fluorescent Dyes; D009961:Orosomucoid; D011798:Quinaldines; D000735:Androstenedione; C021324:quinaldine red