Separation of pig liver esterase isoenzymes and subunits by capillary zone electrophoresis in the presence of fluorinated surfactants.
An attempt was made to separate the isoenzymes and subunits of pig liver esterase by capillary zone electrophoresis. This enzyme is a complex mixture and is strongly adsorbed on a fused-silica capillary. However, by simply adding a cationic fluorosurfactant to the running buffer, adsorption was significantly reduced. The effects of adding a zwitterionic and a neutral fluorosurfactant were also investigated. Large changes in the elution pattern were observed when using different combinations of these additives. Mixtures of different fluorosurfactants added to the running buffer can therefore be utilized in strategies for optimization of the separation selectivity.
Emmer
A
A
Department of Analytical Chemistry, Royal Institute of Technology, Stockholm, Sweden.
Jansson
M
M
Roeraade
J
J
D000818:Animals; D004586:Electrophoresis / Q000379:methods*; D004950:Esterases / Q000032:analysis*; D005461:Fluorine; D007527:Isoenzymes / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D013501:Surface-Active Agents; D013552:Swine
D007527:Isoenzymes; D013501:Surface-Active Agents; D005461:Fluorine; D004950:Esterases