Translactational exposure of F1 mouse pups to selenium.
In an investigation of the modulation of certain neonatal xenobiotic-metabolizing enzymes in liver of mouse pups postnatally exposed to selenium through the transmammary route, sodium selenite was administered in drinking water to lactating dams at the dose levels of 1 or 5 ppm from day 1 of lactation and continued daily for 14 or 21 days. The higher dose of selenium was found to increase the hepatic acid-soluble sulfhydryl content significantly after 21 days of treatment in dams, their pups (P < 0.01) and in the 14-day-old male pups (P < 0.05). Cytochrome b5 content decreased in the livers of dams that received 5 ppm selenium (P < 0.01) and in the F1 pups (P < 0.01) translactationally exposed to selenium for 14 days. Cytochrome P-450 content decreased in dams and pups exposed to 5 ppm selenium for 14 days and either dose for 21 days (P < 0.01). Hepatic glutathione S-transferase decreased in the dam that had received 5 ppm selenium for 14 days (P < 0.05) and in the 14-day-old pups (P < 0.01). Glutathione reductase and glutathione peroxidase activities decreased in both dams and pups (P < 0.01). The overall suppression of neonatal hepatic detoxification enzymes demonstrates that selenium may have far-reaching consequences on neonatal growth, development and drug pharmacokinetics.
Chhabra
S K
SK
Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University (J.N.U.), New Delhi, India.
Rao
A R
AR
D000818:Animals; D000831:Animals, Newborn; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D015786:Cytochromes b5 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007774:Lactation; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D012643:Selenium / Q000633:toxicity*; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D013438:Sulfhydryl Compounds; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D012643:Selenium