title: ED-A region-containing isoform of cellular fibronectin is present in dentin matrix in dentinogenesis imperfecta associated with osteogenesis imperfecta.
abstract: To elucidate the defective dentin formation in osteogenesis imperfecta (OI), we analyzed the expression of selected fibronectin (FN) isoforms in the dentin matrix of a patient with dentinogenesis imperfecta (DI) associated with OI, and in normal teeth. Frozen tooth sections were immunostained with three monoclonal antibodies (MAbs). The MAb recognizing the major cell-binding region (f-33), shared by plasma FN (pFN) and cellular FN (cFN), stained the pulp of normal adult permanent teeth intensely, while no reactivity was present in predentin, (demineralized) dentin, or dental cementum. The periodontal ligament stained unevenly. The dentin matrix of the patient with OI displayed reactive zones, alternating layerwise or concentrically with non-reactive ones. Staining throughout the connective tissue of adult oral mucosa, analyzed for the form of FN present, was intense, and in dermis, which was also studied, it was moderate. Reactivities in dental tissues with the MAb specific for the ED-A region (IST-9), included in cFN but not pFN, were similar to those with MAb f-33. The mucosal connective tissue stained weakly and dermis was negative, except that nerves and endothelia of some large blood vessels stained clearly. The MAb specific for the ED-B segment (BC-1), also included in cFN only, did not stain any of the tissues analyzed. The results suggest that, unlike mucosal and dermal FNs, FNs in the dental tissues are largely cellular, and also that dentin formation in OI may be completed by successive generations of pulpal fibroblasts differentiated into hard-tissue-forming cells.
authors.affiliation: Department of Oral Pathology, University of Helsinki, Finland.
authors.forename: P L
authors.initials: PL
authors.lastname: Lukinmaa
authors.forename: A
authors.initials: A
authors.lastname: Vaheri
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D002648:Child; D003782:Dental Pulp / Q000737:chemistry; D003804:Dentin / Q000737:chemistry* / Q000473:pathology; D003810:Dentinogenesis; D003811:Dentinogenesis Imperfecta / Q000150:complications / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009061:Mouth Mucosa / Q000737:chemistry; D009804:Odontoblasts / Q000378:metabolism; D010013:Osteogenesis Imperfecta / Q000150:complications / Q000378:metabolism; D010519:Periodontium / Q000737:chemistry; D011487:Protein Conformation; D012867:Skin / Q000737:chemistry; D014070:Tooth / Q000737:chemistry / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D005353:Fibronectins