title: Tn5401 disruption of the spo0F gene, identified by direct chromosomal sequencing, results in CryIIIA overproduction in Bacillus thuringiensis.
abstract: The Bacillus thuringiensis spo0F gene was identified by chromosomal DNA sequencing of sporulation mutants derived from a B. thuringiensis transposon insertion library. A spo0F defect in B. thuringiensis, which was suppressed by multicopy hknA or kinA, resulted in the overproduction of the CryIIIA insecticidal crystal protein.
authors.affiliation: Ecogen Inc., Langhorne, Pennsylvania 19047-1810.
authors.forename: T
authors.initials: T
authors.lastname: Malvar
authors.forename: J A
authors.initials: JA
authors.lastname: Baum
mesh_terms: D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D001427:Bacterial Toxins; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements / Q000235:genetics; D004731:Endotoxins / Q000096:biosynthesis*; D005798:Genes, Bacterial / Q000235:genetics*; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D014158:Transcription, Genetic
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D004731:Endotoxins; D006460:Hemolysin Proteins; C080951:Spo0F protein, Bacillus subtilis; C030956:insecticidal crystal protein, Bacillus Thuringiensis