title: Molecular cloning and expression of two horse pancreatic cDNA encoding colipase A and B.
abstract: Pancreatic colipase plays an essential role in the intestinal fat digestion by anchoring lipase on lipid/water interfaces in the presence of bile salts. In contrast to other species, two molecular forms of colipase, A and B, have been found in horse. The two corresponding cDNAs were isolated from a horse pancreatic library and their nucleotide sequences were determined. Moreover, for the first time, active colipase has been obtained after transfection of COS cells by either colipase A or B cDNA.
authors.affiliation: Laboratoire de Unité de Bioénergétique et d'Ingénieric des Proteines/BIP, CNRS, Marseille, France.
authors.forename: I
authors.initials: I
authors.lastname: Crenon
authors.forename: S
authors.initials: S
authors.lastname: Granon
authors.forename: C
authors.initials: C
authors.lastname: Chapus
authors.forename: B
authors.initials: B
authors.lastname: Kerfelec
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003089:Colipases / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006736:Horses; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D014162:Transfection
chemical_list: D003089:Colipases; D018076:DNA, Complementary