[Diagnosis of falciparum malaria by polymerase chain reaction].
Polymerase Chain Reaction (PCR) method using a pair of Plasmodium falciparum-specific oligonucleotides as primers was used to detect malaria patient blood samples collected from Yunnan endemic malarious area. The results showed that a DNA fragment of 492 bp was amplified in all 53 microscopically diagnosed P. falciparum samples, but negative in 8 P. vivax samples and 12 normal blood samples. This method seems to have high sensitivity and high specificity.
Zhan
B
B
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhang
L X
LX
Wang
J J
JJ
Feng
X P
XP
D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000032:analysis; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction
D016054:DNA, Protozoan