title: Trolox protects hyperglycemia-induced cataractogenesis in cultured rat lens.
abstract: Hyperglycemia-induced cataractogenesis has been studied in rat lenses cultured in 50 mM glucose using an inverted microscope connected with a Universal C-mount and a CCD camera. Digital images were acquired and the opacity was determined by quantitating the transmitted light. Antioxidants, butylated hydroxy toluene (BHT) and 6-hydroxy-2,5,7,8-tetramethenyl-chroman-2-carboxylic acid (Trolox) provided good protection against 50 mM glucose-induced cataractogenesis in rat lenses for upto 8 days. Sorbitol levels in the 50 mM glucose+antioxidant groups were approximately 1.5 mM fold higher than in 50 mM glucose. The results, besides further demonstrating that oxidative damage is the major mechanism of sugar-induced cataractogenesis, show that Trolox or related amphipathic compounds could be of therapeutic use in the prevention of diabetic cataracts.
authors.affiliation: Department of Ophthalmology, University of Texas Medical Branch, Galveston 77555.
authors.forename: N H
authors.initials: NH
authors.lastname: Ansari
authors.forename: A
authors.initials: A
authors.lastname: Bhatnagar
authors.forename: E
authors.initials: E
authors.lastname: Fulep
authors.forename: P
authors.initials: P
authors.lastname: Khanna
authors.forename: S K
authors.initials: SK
authors.lastname: Srivastava
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002084:Butylated Hydroxytoluene / Q000627:therapeutic use; D002386:Cataract / Q000209:etiology* / Q000517:prevention & control*; D002478:Cells, Cultured; D002839:Chromans / Q000627:therapeutic use*; D004347:Drug Interactions; D006943:Hyperglycemia / Q000150:complications*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013012:Sorbitol / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D002839:Chromans; D002084:Butylated Hydroxytoluene; D013012:Sorbitol; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid