Chloramphenicol extraction from milk by using the diphasic dialysis method followed by liquid chromatographic determination.
An economical, fast, and simple method for the extraction and liquid chromatographic determination of chloramphenicol (CAP) in milk is described. CAP is extracted by using a recently developed membrane-based method named "diphasic dialysis." CAP is detected and quantitated in the organic solvent used in dialysis without additional cleanup steps by reversed-phase liquid chromatography and UV detection (270 nm). The determination limit of CAP in milk was about 5 micrograms/L, although as little as 1 microgram/L could be detected under optimal working conditions.
Bayo
J
J
Universidad Complutense, Facultad de Veterinaria, Departamento Patología Animal I, Madrid, Spain.
Moreno
M A
MA
Prieta
J
J
Díaz
S
S
Suárez
G
G
Domínguez
L
L
D000818:Animals; D002701:Chloramphenicol / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D003956:Dialysis / Q000379:methods*; D008892:Milk / Q000737:chemistry*
D002701:Chloramphenicol