[Amplification of DNA for 15-30 minutes in single-use pipette tips].
Amplification of 200-1000-bp DNA fragments was performed in 15-30 min using a rapid thermal cycler based on the commercial instrument TC-1000-1 (IRLEN, St. Petersburg, Russia). Plastic pipette tips were used as thin walled, high surface to volume-ratio tubes, to increase the rate of heating (cooling) of 20 microliters samples, which allowed the time required for DNA amplification to be considerably reduced (5-10 times).
Tret'iakov
A N
AN
Gel'fand
V M
VM
Pantina
R A
RA
Shevtsov
S P
SP
Bulat
S A
SA
D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D012568:Schizosaccharomyces / Q000235:genetics
D017931:DNA Primers; D004271:DNA, Fungal; D004247:DNA
Amplifikatsiia DNK za 15-30 minut v odnorazovykh nakonechnikakh dlia mikrodozatorov.