Kinetic studies of formaldehyde binding in tissue.
Specimens of rabbit liver were fixed for various periods up to 6 days in buffered 14C-formaldehyde. Binding of the isotope reached a plateau after fixation for approximately 24 hr; the half-maximal binding level was reached after approximately 100 min. Formaldehyde binding at 37 C was faster than at 25 C, and faster at pH 7.0 than at pH 4.0. During rinsing of the fixed tissue in water for up to 26 days there was a progressive decrease in isotope content to 10-20% of the pre-rinse level, indicating that formaldehyde fixation is a reversible process.
Helander
K G
KG
Laboratory of Membrane Biology, University of California, Los Angeles 90073.
D000818:Animals; D002250:Carbon Radioisotopes; D005557:Formaldehyde / Q000378:metabolism* / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011817:Rabbits; D013696:Temperature; D016707:Tissue Fixation / Q000379:methods
D002250:Carbon Radioisotopes; D005557:Formaldehyde