Universal phosphorescence immunoassay.
The aim of this study is to develop a universal phosphorescence immunoassay method using monoclonal antibodies to Pd-coproporphyrin (Pd-CP) and conjugates of various proteins with Pd-CP. Pd-CP and monoclonal antibodies obtained allow a convenient method for the determination of various antigens to be developed. The conditions for immunological reactions with Pd-CP were optimized with respect to the components affecting the nonspecific binding Pd-CP and Pd-CP conjugates.
Mantrova EYu
Institute of Biochemistry, Russian Academy of Sciences, Moscow.
Demcheva
M V
MV
Savitsky
A P
AP
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D003306:Coproporphyrins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007118:Immunoassay / Q000379:methods*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D051379:Mice / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013954:Thyroglobulin / Q000032:analysis*; D014325:Tromethamine; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000941:Antigens; D002593:Cetrimonium Compounds; D003306:Coproporphyrins; D007202:Indicators and Reagents; C057160:palladium coproporphyrin; D014325:Tromethamine; D013954:Thyroglobulin; D014360:Trypsin Inhibitor, Kunitz Soybean; D000077286:Cetrimonium