Degradation of a polymerase chain reaction (PCR) product by heat-stable deoxyribonuclease (DNase) produced from Yersinia enterocolitica.
When crude deoxyribonucleic acid (DNA) preparations by boiling were used for the polymerase chain reaction (PCR) from pathogenic and non-pathogenic Yersinia enterocolitica strains, the amplified products were degraded after their storage at 4 C. The degradation of products was prevented by the addition of ethylenediaminetetraacetate (EDTA) or treatment with proteinase K. These findings indicate that Y. enterocolitica produced heat-stable deoxyribonuclease (DNase). Proteinase K treatment would be recommended to prevent heat-stable DNase contamination in the DNA preparations for PCR from Y. enterocolitica strains.
Nakajima
H
H
Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Japan.
Itoh
K
K
Arakawa
E
E
Inoue
M
M
Mori
T
T
Watanabe
H
H
D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004340:Drug Contamination; D004492:Edetic Acid; D019286:Endopeptidase K; D004795:Enzyme Stability; D006358:Hot Temperature; D016133:Polymerase Chain Reaction / Q000379:methods*; D012697:Serine Endopeptidases; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity
D004269:DNA, Bacterial; D004492:Edetic Acid; D003851:Deoxyribonucleases; D012697:Serine Endopeptidases; D019286:Endopeptidase K