The hydrolysis of oestrone sulphate and dehydroepiandrosterone sulphate by human steroid sulphatase expressed in transfected COS-1 cells.
Whether the same or distinct steroid sulphatases (STS) are involved in the hydrolysis of alkyl and aryl steroid sulphates remains controversial. We have examined the ability of a placental steroid sulphatase to hydrolyse oestrone sulphate and/or dehydroepiandrosterone sulphate (DHA-S) by expressing the enzyme in COS-1 cells. Using either intact cells or broken cell preparations, the expressed sulphatase was found to hydrolyse both oestrone sulphate and DHA-S. The catalysis of oestrone sulphate and DHA-S by the expressed sulphatase was almost completely abolished by the steroid sulphatase inhibitor, oestrone-3-O-sulphamate. It is concluded that both alkyl and aryl steroid sulphates can be hydrolysed by the same steroid sulphatase.
Purohit
A
A
Unit of Metabolic Medicine, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, England.
Dauvois
S
S
Parker
M G
MG
Potter
B V
BV
Williams
G J
GJ
Reed
M J
MJ
D000818:Animals; D001192:Arylsulfatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002384:Catalysis; D002460:Cell Line; D002522:Chlorocebus aethiops; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000378:metabolism; D019314:Dehydroepiandrosterone Sulfate; D004966:Estrogens, Conjugated (USP) / Q000378:metabolism*; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D006801:Humans; D006868:Hydrolysis; D010920:Placenta / Q000201:enzymology; D043266:Steryl-Sulfatase; D014162:Transfection
D004966:Estrogens, Conjugated (USP); C088768:estrone-3-O-sulfamate; D004970:Estrone; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D001192:Arylsulfatases; D043266:Steryl-Sulfatase; C017296:estrone sulfate