title: A T7 promoter vector with a transcriptional terminator for stringent expression of foreign genes.
abstract: An expression vector, pKK233-2 [Amann and Brosius, Gene 40 (1985) 183-190], was modified by replacing the trc promoter with the T7 phi 10 promoter and introducing a strong transcriptional terminator upstream from this promoter. This modification successfully suppressed background transcription and resulted in strict dependence upon induction.
authors.affiliation: Department of Biochemistry, Kawasaki Medical School, Okayama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishihara
authors.forename: T
authors.initials: T
authors.lastname: Iwabuchi
authors.forename: T
authors.initials: T
authors.lastname: Nohno
mesh_terms: D017123:Bacteriophage T7 / Q000235:genetics*; D001483:Base Sequence; D004274:DNA, Recombinant; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant