[Expression of hybrid genes containing sequences coding for human adrenocorticotropic hormone in Escherichia coli strains].
E. coli strains producing a hybrid protein containing human adrenocorticotropic hormone (ACTH) and protein A of S. aureus were obtained. The sequence coding for ACTG was obtained from the bovine one using oligonucleotide-directed mutagenesis. The ACTG gene was linked with the protein A gene and its derivatives by synthetic adaptors. It was shown that each of the constructed plasmids directed the synthesis of hybrid protein in E. coli. This protein was purified on IgG-Sepharose. Then ACTH was obtained by HPLC after specific hydrolysis. The amino acid composition of purified sample was determined.
Karapetian
V E
VE
Parsadanian
A Sh
ASh
Galoian
A A
AA
D000324:Adrenocorticotropic Hormone / Q000235:genetics*; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics*
D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins; D000324:Adrenocorticotropic Hormone
Izuchenie ékspressii gibridnykh genov, soderzhashchikh posledovatel'nost', kodiruiushchuiu adrenokortikotropnyĭ gormon cheloveka v shtammakh Escherichia coli.