[Study of conditions for cleaving bovine leukemia virus RNA in vitro using ribozymes directed against long terminal repeats].
Ribozyme has been constructed by the oligonucleotide synthesis technique that is capable of splitting bovine leukemia virus (BLV) RNA-substrate in the R-region (358th nucleotide). Ribozyme has been shown to efficiently split the RNA containing R-region of the virus. The efficiency of ribozyme function depends on conditions of the reaction reaching its maximum at 25 degrees C and pH 7.3-7.6. The efficiency of splitting is increased by multiple heating-cooling cycles of the substrate-ribozyme mixture.
Zelivianskiĭ
S V
SV
Karpov
V A
VA
Baurin
V V
VV
Miroshnichenko
O I
OI
Tikhonenko
T I
TI
D001483:Base Sequence; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D016337:RNA, Catalytic / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D013696:Temperature
D016337:RNA, Catalytic; D012367:RNA, Viral
Izuchenie usloviĭ rasshchepleniia RNK virusa leĭkoza korov in vitro ribozimom, napravlennym protiv dlinnykh kontsevykh povtorov.