title: Isolation of an adhesin from Staphylococcus aureus that binds Lewis a blood group antigen and its relevance to sudden infant death syndrome.
abstract: A 67 kDa protein was isolated from cell membrane preparations of Staphylococcus aureus (NCTC 10655) by affinity adsorption with synthetic Lewis a antigen conjugated to Synsorb beads. Pre-treatment of buccal epithelial cells expressing Lewis a with the purified protein reduced binding of the staphylococcal strain to a greater extent than the material not bound to the Synsorb beads. The significance of this work is discussed with reference to expression of Lewis a antigen in infants and the proposed role of toxigenic strains of staphylococci in some cases of sudden infant death syndrome.
authors.affiliation: Department of Medical Microbiology, University of Edinburgh, Medical School, UK.
authors.forename: A T
authors.initials: AT
authors.lastname: Saadi
authors.forename: D M
authors.initials: DM
authors.lastname: Weir
authors.forename: I R
authors.initials: IR
authors.lastname: Poxton
authors.forename: J
authors.initials: J
authors.lastname: Stewart
authors.forename: S D
authors.initials: SD
authors.lastname: Essery
authors.forename: C C
authors.initials: CC
authors.lastname: Blackwell
authors.forename: M W
authors.initials: MW
authors.lastname: Raza
authors.forename: A
authors.initials: A
authors.lastname: Busuttil
mesh_terms: D018829:Adhesins, Bacterial; D001422:Bacterial Adhesion / Q000276:immunology* / Q000502:physiology*; D001667:Binding, Competitive; D002473:Cell Wall / Q000737:chemistry; D002610:Cheek; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007223:Infant; D007983:Lewis Blood Group Antigens / Q000378:metabolism*; D009061:Mouth Mucosa / Q000166:cytology; D013211:Staphylococcus aureus / Q000276:immunology*; D013398:Sudden Infant Death / Q000209:etiology
chemical_list: D018829:Adhesins, Bacterial; D007983:Lewis Blood Group Antigens; C048165:adhesin, Staphylococcus aureus