Serotyping of human rotavirus by enzyme immuno-assay with monoclonal antibodies.
Two series of enzyme immunoassays with monoclonal antibodies produced in two laboratories (A and B) were compared in use for serotyping of human rotavirus in stool samples collected in Japan between 1988 and 1991 from patients with gastroenteritis. Of 358 samples, 222 were determined to be the same serotype, while 61 samples could not be serotyped by either assay. A hundred and one and 92 samples were not serotyped by the A and B antibodies, respectively. We believe that the A and B monoclonal antibodies are useful clinically for serotyping rotaviruses at the present time.
Hasegawa
A
A
Department of Epidemiology, National Institute of Health, Tokyo.
Mukoyama
A
A
Akatani
K
K
Ikegami
N
N
Urasawa
S
S
Ushijima
H
H
D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology*; D016679:Genome, Viral; D006801:Humans; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000145:classification*; D012703:Serotyping
D000911:Antibodies, Monoclonal; D012367:RNA, Viral