Bioassay quantitation of ramoplanin in human and in nonprimate sera.
This study developed and validated a bioassay for ramoplanin in human, dog, rabbit, and rat sera. Mean analyte recoveries and coefficients of variation for duplicate assays with each serum using coded and spiked (30-720 ng/ml) samples ranged from 93.5% to 106.3% and from 1.8% to 5.4% respectively. All correlation coefficients were > 0.99 and, for each serum, there was no significant difference in overall analyte recovery between the 2 test days.
Kenny
M T
MT
Microbiology Department, Marion Merrell Dow Inc., Kansas City, Missouri 64137.
Brackman
M A
MA
Dulworth
J K
JK
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D001681:Biological Assay; D047630:Depsipeptides; D004285:Dogs; D005069:Evaluation Studies as Topic; D006801:Humans; D010456:Peptides, Cyclic; D011817:Rabbits; D051381:Rats; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D047630:Depsipeptides; D010456:Peptides, Cyclic; C041438:ramoplanin