title: Conjugal transfer of the vancomycin resistance determinant vanB between enterococci involves the movement of large genetic elements from chromosome to chromosome.
abstract: Resistance to various levels of vancomycin and susceptibility to teicoplanin in enterococci (VanB phenotype) is mediated by the vanB gene cluster. VanB-type resistance was transferred by intra- and inter-specific conjugation between different strains of Enterococcus. Analysis of SfiI-digested genomic DNA by zero integrated-field electrophoresis followed by Southern hybridization revealed vanB-containing chromosomal insertions of approximately 90-250 kb in the transconjugants. Thus, transfer of VanB-type resistance is associated with the movement of large genetic elements from chromosome to chromosome.
authors.affiliation: Unité des Agents Antibactériens, CNRS EPJ0058, Institut Pasteur, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Quintiliani
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D003227:Conjugation, Genetic / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D013293:Enterococcus faecalis / Q000235:genetics*; D014640:Vancomycin
chemical_list: D004269:DNA, Bacterial; D014640:Vancomycin