A comparison of Southern blot and polymerase chain reaction as techniques to identify loss of DNA heterozygosity in colorectal cancer.
Alterations in the tumour suppressor gene p53 have been found in a wide variety of tumours. The variable number tandem repeat present at the YNZ22 locus is a highly polymorphic marker closely linked to the p53 gene. Examination for loss of heterozygosity at the YNZ22 locus has been carried out by Southern blot or polymerase chain reaction (PCR). A comparison of these techniques shows a 100% concordance rate in normal tissue but a 90.9% rate in tumour tissue. Both false positive and false negative results were obtained. This must be considered in the interpretation of studies using PCR for this purpose.
Hewett
P J
PJ
Department of Surgery, Flinders Medical Centre, Bedford Park, South Australia.
Firgaira
F
F
Morley
A
A
D000483:Alleles; D015139:Blotting, Southern; D002886:Chromosomes, Human, Pair 17; D003110:Colonic Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D017353:Gene Deletion; D016158:Genes, p53 / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D012004:Rectal Neoplasms / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D004273:DNA, Neoplasm