The UGA4 UASNTR site required for GLN3-dependent transcriptional activation also mediates DAL80-responsive regulation and DAL80 protein binding in Saccharomyces cerevisiae.
Expression of the nitrogen catabolic genes in Saccharomyces cerevisiae, including those of the gamma-aminobutyric acid (UGA) and allantoin (DAL) pathways, is regulated positively by the GLN3 protein and negatively by the DAL80 protein. The deduced sequences of the DAL80 and GLN3 proteins contain a zinc finger motif homologous to those shown to bind GATA sequences. In addition, DAL80 protein has been directly shown to bind to a pair of GATA-containing sequences (URSGATA) in vitro, and a pair of GATA-containing sequences (UASNTR) is required for GLN3-dependent transcriptional activation in a heterologous expression vector. We demonstrate here that the GATA-containing sites upstream of UGA4 required for optimal GLN3-dependent transcriptional activation also mediate DAL80 protein binding in vitro and DAL80-responsive regulation in vivo.
Cunningham
T S
TS
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Dorrington
R A
RA
Cooper
T G
TG
D000612:4-Aminobutyrate Transaminase / Q000235:genetics; D000481:Allantoin / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D050485:GABA Plasma Membrane Transport Proteins; D050980:GATA Transcription Factors; D015966:Gene Expression Regulation, Fungal; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D027361:Organic Anion Transporters; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012097:Repressor Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D014158:Transcription, Genetic; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D002352:Carrier Proteins; C071662:DAL80 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D050485:GABA Plasma Membrane Transport Proteins; D050980:GATA Transcription Factors; C071664:GLN3 protein, S cerevisiae; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C513599:UGA4 protein, S cerevisiae; D000481:Allantoin; D005680:gamma-Aminobutyric Acid; D000612:4-Aminobutyrate Transaminase