title: Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides.
abstract: UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.
authors.affiliation: Institute for Medical Physics and Biophysics, Münster, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Nordhoff
authors.forename: F
authors.initials: F
authors.lastname: Kirpekar
authors.forename: M
authors.initials: M
authors.lastname: Karas
authors.forename: R
authors.initials: R
authors.lastname: Cramer
authors.forename: S
authors.initials: S
authors.lastname: Hahner
authors.forename: F
authors.initials: F
authors.lastname: Hillenkamp
authors.forename: K
authors.initials: K
authors.lastname: Kristiansen
authors.forename: P
authors.initials: P
authors.lastname: Roepstroff
authors.forename: A
authors.initials: A
authors.lastname: Lezius
mesh_terms: D001483:Base Sequence; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010848:Picolinic Acids; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013386:Succinates; D019802:Succinic Acid
chemical_list: D009838:Oligodeoxyribonucleotides; D010848:Picolinic Acids; D013386:Succinates; C049246:3-hydroxypicolinic acid; D019802:Succinic Acid