title: The Escherichia coli RuvB branch migration protein forms double hexameric rings around DNA.
abstract: The RuvB protein is induced in Escherichia coli as part of the SOS response to DNA damage. It is required for genetic recombination and the postreplication repair of DNA. In vitro, the RuvB protein promotes the branch migration of Holliday junctions and has a DNA helicase activity in reactions that require ATP hydrolysis. We have used electron microscopy, image analysis, and three-dimensional reconstruction to show that the RuvB protein, in the presence of ATP, forms a dodecamer on double-stranded DNA in which two stacked hexameric rings encircle the DNA and are oriented in opposite directions with D6 symmetry. Although helicases are ubiquitous and essential for many aspects of DNA repair, replication, and transcription, three-dimensional reconstruction of a helicase has not yet been reported, to our knowledge. The structural arrangement that is seen may be common to other helicases, such as the simian virus 40 large tumor antigen.
authors.affiliation: Laboratory of Ultrastructural Analysis, University of Lausanne, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Stasiak
authors.forename: I R
authors.initials: IR
authors.lastname: Tsaneva
authors.forename: S C
authors.initials: SC
authors.lastname: West
authors.forename: C J
authors.initials: CJ
authors.lastname: Benson
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: E H
authors.initials: EH
authors.lastname: Egelman
mesh_terms: D000465:Algorithms; D001426:Bacterial Proteins / Q000648:ultrastructure*; D004265:DNA Helicases / Q000648:ultrastructure*; D004269:DNA, Bacterial / Q000648:ultrastructure*; D004926:Escherichia coli / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D008855:Microscopy, Electron, Scanning; D008958:Models, Molecular
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C061260:RuvB protein, Bacteria; D004265:DNA Helicases