title: Alternative cell fate choice induced by low-level expression of a regulator of protein phosphatase 2A in the Drosophila peripheral nervous system.
abstract: The Drosophila gene twins encodes the regulatory B subunit of type 2A protein phosphatase. Here we report that its partial loss-of-function mutations caused abnormal morphogenesis in the adult peripheral nervous system. In wild-type flies, the mechanoreceptor, one major class of sensory organs, is composed of four specialized cells (one neuron and three accessory cells) that are derived from a single precursor cell. The hypomorphic twins mutations did not block division of this precursor, but most likely altered cell fate in this lineage to produce only accessory cells that form sensory structures. Stepwise reductions of twins protein enhanced this transformation. In these mutants, another regulatory subunit, A, and the catalytic subunit, C, of the phosphatase were expressed at normal levels. Therefore, the modulation of the phosphatase activity by the B subunit appears to be crucial for specification of neural cell identity.
authors.affiliation: Department of Biophysics, Faculty of Science, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shiomi
authors.forename: M
authors.initials: M
authors.lastname: Takeichi
authors.forename: Y
authors.initials: Y
authors.lastname: Nishida
authors.forename: Y
authors.initials: Y
authors.lastname: Nishi
authors.forename: T
authors.initials: T
authors.lastname: Uemura
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D017344:Genes, Insect / Q000235:genetics*; D008969:Molecular Sequence Data; D017933:Peripheral Nervous System / Q000196:embryology* / Q000254:growth & development; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D054648:Protein Phosphatase 2
chemical_list: D017931:DNA Primers; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2