Enhanced competitiveness of tomato bushy stunt virus defective interfering RNAs by segment duplication or nucleotide insertion.
We have analyzed atypical tomato bushy stunt virus defective interfering (DI) RNA species which accumulated during a passage series in protoplasts. We present a rationale for the order of appearance of these molecules and show, using competition assays, that either segment duplication or single nucleotide insertion can enhance DI RNA competitiveness. Possible mechanisms for the introduction of the modifications observed in these DI RNAs are discussed.
White
K A
KA
School of Biological Sciences, University of Nebraska-Lincoln 68588-0118.
Morris
T J
TJ
D001483:Base Sequence; D002478:Cells, Cultured; D003673:Defective Viruses; D015967:Gene Expression Regulation, Viral; D006860:Hydrogen Bonding; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000235:genetics*; D017864:Tombusvirus / Q000235:genetics*; D014675:Vegetables; D014762:Viral Interference; D014779:Virus Replication
D012367:RNA, Viral