title: Crystallization and preliminary X-ray diffraction studies of an endoglucanase from Aspergillus aculeatus.
abstract: Most fungal cellulases are found in multiple forms varying in size and substrate specificity. Aspergillus aculeatus is known to produce nine cellulolytic enzymes including an endoglucanase (FI CM-cellulase, M(r) = 24,002) as the major component. Single crystals of FI CM-cellulase from Aspergillus aculeatus have been prepared by sitting-drop vapour diffusion using ammonium sulphate as a precipitant. The cellulase crystals belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions of a = 52.79(2) A, b = 106.40(4) A and c = 33.15(1) A. The crystals contain one enzyme molecule per asymmetric unit. They diffract to at least 2.0 A resolution and are very stable against X-ray irradiation.
authors.affiliation: Institute for Chemical Research, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hata
authors.forename: K
authors.initials: K
authors.lastname: Natori
authors.forename: Y
authors.initials: Y
authors.lastname: Katsube
authors.forename: T
authors.initials: T
authors.lastname: Ooi
authors.forename: M
authors.initials: M
authors.lastname: Arai
authors.forename: H
authors.initials: H
authors.lastname: Okada
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D001230:Aspergillus / Q000201:enzymology*; D002480:Cellulase / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight
chemical_list: D002480:Cellulase; D000645:Ammonium Sulfate