Transcription of the human apolipoprotein A-II is down-regulated by the first intron of its gene.
Several reports indicate that apoA-II, the second most abundant HDL protein, plays a crucial role in modulating the anti-atherogenic behavior of HDL. Regulatory elements located 5' to the human apoA-II promoter have been previously described. In this paper we report that the first intron of the human apoA-II gene down-regulates its own promoter and the ubiquitous thymidine kinase promoter both in HepG2 and Caco-2 cells. The intron contains three sequences which bind nuclear proteins, thus demonstrating the presence of regulatory elements downstream of the transcription start site of the apoA-II gene.
Bossu
J P
JP
SERLIA, Institut Pasteur de Lille, France.
Chartier
F L
FL
Vu-Dac
N
N
Fruchart
J C
JC
Laine
B
B
D016633:Apolipoprotein A-II / Q000235:genetics*; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular; D003110:Colonic Neoplasms; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns; D008113:Liver Neoplasms; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D016633:Apolipoprotein A-II; D009687:Nuclear Proteins; D013937:Thymidine Kinase; D003850:Deoxyribonuclease I