title: [The development of a technology for the production of live dried vaccines against avian pasteurellosis and swine erysipelas].
abstract: The technology of the production of dried live vaccine against Pasteurella infection of fowl from Pasteur's 2nd avirulent strain, strains AB and K, has been developed. This technology includes the process of batch cultivation of Pasteurella cells, controlled in such parameters as eH, pO2 and glucose concentration, in fermenters in optimized culture medium, based on Hottinger hydrolysate and fermentative casein-yeast hydrolysate, and preservation in improved saccharose-gelatin medium prepared in potassium sulfate buffer solution. The new technology makes it possible to increase the yield of preparations with stable biological activity 5- to 13-fold in comparison with the traditional technology. Furthermore, the technology of the production of live dried vaccine against swine erysipelas from Erysipelothrix insidiosa strain BP-2 has been developed. This technology is based on maintaining the optimum conditions of the batch cultivation of E. insidiosa in meat medium based on Hottinger hydrolysate and media obtained from hydrolysate of pancreatic fermentation products of microbial biomass; the preparation thus obtained is stabilized in peptone-saccharose-gelatin medium prepared in potassium phosphate buffer solution. This increases the yield of the vaccine 8-fold in comparison with the traditional technology, while ensuring the stability of bacteria after drying and during prolonged storage.
authors.forename: M Ia
authors.initials: MIa
authors.lastname: Iartsev
authors.forename: I A
authors.initials: IA
authors.lastname: Basnak'ian
authors.forename: A A
authors.initials: AA
authors.lastname: Raevskiĭ
authors.forename: E P
authors.initials: EP
authors.lastname: Sapegina
authors.forename: V P
authors.initials: VP
authors.lastname: Shishov
authors.forename: S P
authors.initials: SP
authors.lastname: Rogozhin
authors.forename: E F
authors.initials: EF
authors.lastname: Tokarik
authors.forename: A A
authors.initials: AA
authors.lastname: Maslak
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology / Q000302:isolation & purification*; D002645:Chickens; D003470:Culture Media; D004353:Drug Evaluation, Preclinical / Q000662:veterinary; D004888:Erysipelothrix / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary*; D016979:Pasteurella multocida / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D011201:Poultry Diseases / Q000517:prevention & control*; D013552:Swine; D013554:Swine Erysipelas / Q000517:prevention & control*; D014613:Vaccines, Attenuated / Q000276:immunology / Q000302:isolation & purification; D014774:Virulence
chemical_list: D001428:Bacterial Vaccines; D003470:Culture Media; D014613:Vaccines, Attenuated
vernacular_title: Razrabotka tekhnologii proizvodstva zhivykh sukhikh vaktsin protiv pasterelleza ptits i rozhi svineĭ.