title: High-molecular-weight proteins of nontypeable Haemophilus influenzae mediate bacterial adhesion to cellular proteoglycans.
abstract: A family of high-molecular-weight (HMW) surface-exposed proteins of nontypeable Haemophilus influenzae (NT H. influenzae) mediated adherence of these organisms to human epithelium. To better understand the molecular basis for this adherence, the role of glycosaminoglycans (GAGs), substances commonly expressed on cell surfaces, was examined. Bacterial adherence to cells with specific deficiencies in GAG biosynthesis was measured. HMW protein-dependent bacterial adherence to normal cells was significantly greater than adherence to cells deficient in sulfated GAGs or to cells deficient in heparan sulfate but overexpressing chondroitin sulfate. Cells expressing undersulfated heparan sulfate exhibited intermediate levels of bacterial adherence. The addition of exogenous dextran sulfate or heparin inhibited over 70% of the adherence of NT H. influenzae to normal cells, whereas hyaluronic acid and chondroitin sulfate tested at the same concentration (100 micrograms/ml) inhibited bacterial adherence by less than 11%. Treatment of cells with heparinase significantly reduced bacterial adherence. Following electrophoretic separation, HMW proteins were shown to bind directly to radiolabeled heparin. These results indicate that HMW protein-dependent adherence of NT H. influenzae is mediated by cellular sulfated GAGs and that heparan sulfate may be the predominant GAG involved in this process. However, the decreased adherence of bacteria to cells expressing undersulfated heparan sulfate and the inhibition of bacterial adherence by the addition of exogenous dextran sulfate suggest that bacterial adhesion to mammalian cells is likely to be influenced by a variety of factors, including the degree of sulfation and the specificity of the carbohydrate moieties contained in the cellular proteoglycans.
authors.affiliation: Department of Pediatrics, Cornell University Medical College, New York, New York 10021.
authors.forename: G J
authors.initials: GJ
authors.lastname: Noel
authors.forename: D C
authors.initials: DC
authors.lastname: Love
authors.forename: D M
authors.initials: DM
authors.lastname: Mosser
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000502:physiology*; D016466:CHO Cells; D006224:Cricetinae; D016264:Dextran Sulfate / Q000494:pharmacology; D006193:Haemophilus influenzae / Q000472:pathogenicity*; D006493:Heparin / Q000378:metabolism; D008970:Molecular Weight; D011509:Proteoglycans / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D011509:Proteoglycans; D006493:Heparin; D016264:Dextran Sulfate