Nucleotide sequence analysis of two 5-nitroimidazole resistance determinants from Bacteroides strains and of a new insertion sequence upstream of the two genes.
DNA sequence analysis of regions from plasmid pIP417 and chromosome BF8 which encode 5-nitroimidazole resistance in Bacteroides strains allowed the identification of two open reading frames corresponding to new genes, nimA (528 bp) and nimB (492 bp). Either gene may confer 5-nitroimidazole resistance to susceptible strains of Bacteroides. The encoded polypeptides have deduced molecular masses of 20.1 and 18.6 kDa, respectively, and share about 73% identity and 85% similarity. A new insertion sequence (IS) element named IS1168 lies 14 bases upstream of the nimA gene. The complete sequence of IS1168 was determined. A similar IS exists 12 bp upstream of the nimB gene. About 60% of the BF8 IS element was also sequenced and shown to be almost identical to IS1168.
Haggoud
A
A
Unité des Anaérobies, Institut Pasteur, Paris, France.
Reysset
G
G
Azeddoug
H
H
Sebald
M
M
D001439:Bacteroides / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D008969:Molecular Sequence Data; D009593:Nitroimidazoles / Q000494:pharmacology*; D010957:Plasmids
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009593:Nitroimidazoles