Establishment of a new lung sarcoma cell line from a human lung carcinosarcoma.
A cell-line (UACC 2561), was established from a biopsy specimen of a lung carcinosarcoma. The patient had Stage III non-small cell lung cancer. Characterization of this cell line revealed highly aneuploid cells containing multiple clonal chromosome alterations with growth in nude mice within 3 weeks following subcutaneous injection. The cell line UACC 2561 stained positive for vimentin and displayed resistance to a variety of chemotherapeutic agents. Polymerase chain reaction followed by single-strand conformational polymorphism analysis revealed the presence of a K-ras 12 codon mutation. This mutation was confirmed by mutation specific PCR analysis for mutant K-ras alleles and direct DNA sequencing to be a GGT to GTT at codon 12 of the K-ras gene. Examination by PCR-SSCP for p53 mutations did not reveal any point mutations in exon 5-8 of the p53 gene. This relatively rare cell line may represent a useful model to investigate human lung sarcomas.
Leibovitz
A
A
Department of Pathology, College of Medicine, University of Arizona.
Wymer
J
J
Massey
K
K
Thompson
F H
FH
Nelson
M A
MA
D000818:Animals; D002296:Carcinosarcoma / Q000235:genetics* / Q000473:pathology*; D002455:Cell Division; D002869:Chromosome Aberrations / Q000235:genetics; D025063:Chromosome Disorders; D004273:DNA, Neoplasm / Q000737:chemistry; D005091:Exons / Q000235:genetics; D005434:Flow Cytometry; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured / Q000473:pathology
D004273:DNA, Neoplasm