Altered ganglioside expression by SH-SY5Y cells upon retinoic acid-induced neuronal differentiation.
SH-SY5Y Neuroblastoma cells were used to study the effect of retinoic acid (RA)-induced differentiation on the expression of gangliosides and neuronal markers. In the presence of 10 microM RA, more than 70% of the cells differentiate to a neuronal phenotype within 8 days. They extend long neuritic processes and show an enhanced immuno-expression of neurone-specific enolase (NSE), neurofilament protein (NF-M), and polysialic acid (PSA). SH-SY5Y cells were found to express at least 12 different gangliosides. RA-induced neuronal differentiation led to a decrease in the content of GM2, GD3, and GD2 and to a 3-7 fold increased concentration of the ganglio-tetraosyl gangliosides GM1, GD1a, GT1a, GD1b, and GT1b. Thus, RA-induced neuronal differentiation of SH-SY5Y cells is accompanied by ganglioside changes similar to those observed during embryonic neuronal differentiation.
Rebhan
M
M
Institute of Zoology, University of Hohenheim, Stuttgart, Germany.
Vacun
G
G
Bayreuther
K
K
Rösner
H
H
D000911:Antibodies, Monoclonal; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005732:Gangliosides / Q000096:biosynthesis*; D006801:Humans; D009423:Nervous System Neoplasms / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism; D009474:Neurons / Q000187:drug effects*; D009479:Neuropeptides / Q000096:biosynthesis / Q000276:immunology; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D005732:Gangliosides; D009479:Neuropeptides; D014212:Tretinoin