title: Extracellular matrix metalloproteinases around loose total hip prostheses.
abstract: We have explored the tissue localization of extracellular matrix metalloproteinases MMP-1 (fibroblast collagenase), MMP-2 (72-kDa gelatinase/Type IV collagenase), MMP-3 (stromelysin), MMP-8 (polymorphonuclear leukocyte collagenase) and MMP-9 (92-kDa gelatinase/Type IV collagenase) in the tissues around loose hip prostheses. The findings were compared with those in synovial tissues obtained from patients with a fractured femoral neck. MMP-type specific antisera were applied in the sensitive avidin-biotin-peroxidase complex methods. MMP-1 was found in monocyte/macrophages, fibroblasts, and vascular endothelial cells in both interface tissues between bone and acetabular components and the pseudocapsular tissues obtained from loosening of hip prostheses. In these tissues, MMP-8 was occasionally found, but only in polymorphonuclear leukocytes. Cells showing immunoreactivity to 72- and 92-kDa gelatinase/Type IV collagenase, MMP-2 and MMP-9, respectively, and stromelysin, MMP-3, were abundant in both interface and pseudocapsular tissues in loose hip prostheses. In contrast, in hip fractures, immunoreactivity to MMP-1, 2, 3, and 9 was weak and only observed in synovial tissues. Immunoreactivity to MMP-8 was confined to polymorphonuclear leukocytes attached to the synovial membrane or in the infiltrate around blood vessels in the subsynovial connective tissues. The finding of MMP-1, 2, 3, and 9 in the tissues around loose hip prostheses suggests that they play a role in the weakening of connective tissues, and this leads to loosening.
authors.affiliation: Department of Anatomy, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Takagi
authors.forename: Y T
authors.initials: YT
authors.lastname: Konttinen
authors.forename: S
authors.initials: S
authors.lastname: Santavirta
authors.forename: T
authors.initials: T
authors.lastname: Sorsa
authors.forename: A Z
authors.initials: AZ
authors.lastname: Eisen
authors.forename: L
authors.initials: L
authors.lastname: Nordsletten
authors.forename: A
authors.initials: A
authors.lastname: Suda
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D004730:Endothelium, Vascular / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry*; D005260:Female; D006622:Hip Prosthesis; D006801:Humans; D008297:Male; D008666:Metalloendopeptidases / Q000032:analysis*; D008875:Middle Aged; D011475:Prosthesis Failure; D012086:Reoperation
chemical_list: D008666:Metalloendopeptidases