title: Electrical properties of mammalian lens epithelial gap junction channels.
abstract: OBJECTIVE
  To establish an "electrical fingerprint" for the gap junction channels between mammalian lens epithelial cells.
  
  
  METHODS
  The double whole cell patch clamp technique was applied to isolated cell pairs obtained from mouse lens epithelium and a continuous cell line of lens epithelial cells derived from the sheep lens (SLE 2.1).
  
  
  RESULTS
  The junctional conductance in mouse lens epithelial cells and in cultured SLE 2.1 cells was found to be moderately voltage dependent. SLE 2.1 cells were analyzed in more detail. The voltage dependence could be described by a Boltzmann distribution with Vo = +/- 63.1 mV and Gmin = 0.34. In cell pairs that exhibited spontaneously low junctional conductance, single channel events could be distinguished. Single gap junction channel currents had a linear current-voltage relationship. A frequency histogram of single channel conductances from eight cell pairs had three major peaks of 35, 60 and 97 pS.
  
  
  CONCLUSIONS
  The electrical properties of gap junction channels between mammalian lens epithelial cells were virtually identical to those previously reported for transfected cell lines expressing connexin43. The authors' physiological data are therefore in agreement with molecular studies that have identified connexin43 as the major connexin of lens epithelial cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Auckland, New Zealand.
authors.forename: P J
authors.initials: PJ
authors.lastname: Donaldson
authors.forename: M
authors.initials: M
authors.lastname: Roos
authors.forename: C
authors.initials: C
authors.lastname: Evans
authors.forename: E
authors.initials: E
authors.lastname: Beyer
authors.forename: J
authors.initials: J
authors.lastname: Kistler
mesh_terms: D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D018031:Connexin 43 / Q000502:physiology; D004594:Electrophysiology; D004848:Epithelium / Q000502:physiology; D005455:Fluorescent Antibody Technique; D017629:Gap Junctions / Q000502:physiology*; D007908:Lens, Crystalline / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D012756:Sheep
chemical_list: D018031:Connexin 43