[The physicochemical and biological characteristics of the Yersinia pestis pH 6 antigen isolated by an immunosorption method].
The immunosorption method was shown to be suitable for the isolation of antigen pH 6, thus making it possible to obtain a highly active preparation. As eluent, 3.0 M KSCN was used. According to the data of gel filtration, the molecular weight of purified antigen pH 6 exceeds 1 x 10(6) daltons. In SDS disk electrophoresis antigen pH 6 is segregated into subunits of approximately 130,000 daltons. The preparation induces inflammatory reaction if introduced intradermally and possesses hemagglutinating and cytotoxic activity.
Stepanshina
V N
VN
Gremiakova
T A
TA
Anisimov
A P
AP
Potapov
V D
VD
D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D007114:Immunization; D007163:Immunosorbent Techniques; D016676:Macrophages, Alveolar / Q000276:immunology; D008970:Molecular Weight; D010586:Phagocytes / Q000276:immunology; D011817:Rabbits; D013696:Temperature; D015010:Yersinia pestis / Q000276:immunology* / Q000302:isolation & purification
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C081814:pH 6 antigen, Yersinia
Fiziko-khimicheskaia i biologicheskaia kharakteristika pH6-antigena Yersinia pestis, vydelennogo immunosorbtsionnym metodom.