A survey of G6 and G10 serotypes of group A bovine rotaviruses from diarrheic beef and dairy calves using monoclonal antibodies in ELISA.
Group A bovine rotaviruses (BRV) have been identified worldwide as a major cause of diarrhea in the young of many species, including humans. Group A rotaviruses are classified into serotypes on the basis of the outer capsid proteins, VP7 (G types) and VP4 (P types). To date, there are 14 G types of group A rotaviruses, with G1, G6, G8, and G10 described for BRV isolates. In this study, G6- and G10-specific monoclonal antibodies (MAbs) were used in an enzyme-linked immunosorbent assay (ELISA) for the G typing of BRV-positive stool samples from diarrheic beef and dairy calves from South Dakota, Ohio, Michigan, Nebraska, and Washington, USA, and Ontario, Canada. ELISA plates were coated using a broadly reactive VP7 MAb (Common 60) or with G6- or G10-specific MAbs. BRV-positive fecal samples were diluted and added to duplicate wells, followed by the addition of polyclonal guinea pig anti-group A rotavirus serum as the secondary antibody. Several reference G6 and G10 BRV strains as well as other G types previously reported in cattle (G1, G2, G3, G8) and BRV-negative samples were included as G type specificity and negative controls. From a total of 308 field samples analyzed, 79% (244/308) tested positive by the broadly reactive VP7 MAb; of these, 54% (131/244) were G6 positive, 14% (35/244) were G10 positive, 4% (9/244) were both G6 and G10 positive, and 28% (69/244) were G6 and G10 negative.(ABSTRACT TRUNCATED AT 250 WORDS)
Lucchelli
A
A
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Kang
S Y
SY
Jayasekera
M K
MK
Parwani
A V
AV
Zeman
D H
DH
Saif
L J
LJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology*; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests / Q000662:veterinary; D012401:Rotavirus / Q000145:classification* / Q000276:immunology; D012400:Rotavirus Infections / Q000276:immunology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000662:veterinary
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral