Distribution of the enantiomers of hydroxychloroquine and its metabolites in ocular tissues of the rabbit after oral administration of racemic-hydroxychloroquine.
The disposition of the enantiomers of hydroxychloroquine (HCQ) and its major metabolites in ocular tissues of rabbits has been studied. Both albino, New Zealand White (NZW), and pigmented animals were administered daily oral doses of rac-HCQ, (S)-HCQ or (R)-HCQ (20 mg/kg) over 1, 6, or 8 day periods or for 8 days followed by a 7-day washout period. At the end of the study periods, plasma and whole blood samples were collected and the rabbits were sacrificed. The eyes were collected, the aqueous humor removed with a syringe, and the eyes separated into the cornea, lens, vitreous body, iris, choroid-retina, sclera, and conjunctiva. The concentrations of (R)-HCQ, (S)-HCQ, and their respective metabolites were determined using a validated enantioselective liquid chromatographic assay. The data from these studies indicate that HCQ accumulated in both pigmented and nonpigmented ocular tissues. In the pigmented tissues, HCQ and its metabolites were bound to melanin and the binding was not enantiospecific. In the nonpigmented tissues and in the iris and retina-choroid of the NZW rabbits, the accumulation appeared to be the result of a reversible and enantioselective binding of HCQ and its metabolites to an unidentified biopolymer present in these ocular tissues.
Wainer
I W
IW
Department of Oncology, McGill University, Montreal, Quebec, Canada.
Chen
J C
JC
Parenteau
H
H
Abdullah
S
S
Ducharme
J
J
Fieger
H
H
Iredale
J
J
D000284:Administration, Oral; D000818:Animals; D005123:Eye / Q000378:metabolism*; D006886:Hydroxychloroquine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D008543:Melanins / Q000378:metabolism; D011817:Rabbits; D013237:Stereoisomerism; D014018:Tissue Distribution
D008543:Melanins; D006886:Hydroxychloroquine