Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides.
UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.
Nordhoff
E
E
Institute for Medical Physics and Biophysics, Münster, Germany.
Kirpekar
F
F
Karas
M
M
Cramer
R
R
Hahner
S
S
Hillenkamp
F
F
Kristiansen
K
K
Roepstroff
P
P
Lezius
A
A
D001483:Base Sequence; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010848:Picolinic Acids; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013386:Succinates; D019802:Succinic Acid
D009838:Oligodeoxyribonucleotides; D010848:Picolinic Acids; D013386:Succinates; C049246:3-hydroxypicolinic acid; D019802:Succinic Acid