title: Plants transformed with a mutant alfalfa mosaic virus coat protein gene are resistant to the mutant but not to wild-type virus.
abstract: Transgenic tobacco plants expressing the wild-type (wt) coat protein (CP) gene of alfalfa mosaic virus (AIMV) have been shown to be resistant to infection with viral particles and RNAs or to infection with viral particles only. The difference in resistance of these plants to RNA inocula was found to correlate with a difference in the expression level of the transgene. Plants expressing a mutant AIMV CP with the N-terminal serine residue changed to glycine have been shown to be susceptible to infection with wt viral particles or RNAs. By site-directed mutagenesis of AIMV cDNA a viable mutant virus encoding CP with the same N-terminal mutation was obtained. Plants expressing wt or mutant CP were resistant to the mutant virus, demonstrating that a single amino acid substitution in CP did not permit the virus to overcome CP-mediated resistance. Although the mutant CP did not confer resistance to wt virus when expressed in transgenic plants, it was still effective in classical cross-protection: plants infected with the mutant virus were resistant to severe strain of AIMV. A model to explain the data is discussed.
authors.affiliation: Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, The Netherlands.
authors.forename: P E
authors.initials: PE
authors.lastname: Taschner
authors.forename: G
authors.initials: G
authors.lastname: van Marle
authors.forename: F T
authors.initials: FT
authors.lastname: Brederode
authors.forename: N E
authors.initials: NE
authors.lastname: Tumer
authors.forename: J F
authors.initials: JF
authors.lastname: Bol
mesh_terms: D017794:Alfalfa mosaic virus / Q000235:genetics*; D001483:Base Sequence; D002213:Capsid / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000209:etiology*; D012367:RNA, Viral / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D012367:RNA, Viral