[Frequency potentiation mechanisms of cyclic AMP-dependent responses of snail neurons].
Intracellular injection of cAMP with the help of microiontophoresis (5-40 nA, 1-10 s) into land snail neurons induces rapid membrane depolarization. Action potentials have not influence on the following cAMP-responses. When the intervals between cAMP-injections are less than 5 minutes the effect of frequency potentiation of cAMP-responses is obtained. The experiments with cAMP-injection from two different barrels of multibarreled microelectrode show that a change of the microelectrode barrels during repetitive injections causes an abolition of the frequency potentiation effect of cAMP-responses. The results suggest that the frequency potentiation effect of cAMP-responses may be explained by peculiarities of microiontophoresis.
Borisova
O V
OV
Solntseva
E I
EI
Skrebitskiĭ
V G
VG
D000818:Animals; D000242:Cyclic AMP / Q000494:pharmacology*; D006372:Helix, Snails; D007478:Iontophoresis; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D008846:Micromanipulation; D009474:Neurons / Q000187:drug effects*
D000242:Cyclic AMP
Izuchenie mekhanizmov chastotnoĭ potentsiatsii tsAMP-zavisimykh otvetov neĭronov vinogradnoĭ ulitki.