Analysis of oligosaccharides of human IgG from serum of leukemia patients.
The biantennary N-acetyllactosamine type oligosaccharide is attached to asparagine-297 of the human IgG heavy chain, which is an integral part of the tertiary structure of the Fc region, and is quite important in the role of IgG. Pyridylamination and analysis by HPLC can be performed more rapidly and simply than conventional carbohydrate analysis. We applied it to the analysis of the oligosaccharide structures of IgG from the serum of patients with leukemia, CLL and AML. The proportion of oligosaccharide from leukemia patients with a bisecting N-acetylglucosamine residue was almost equal to that of healthy individuals. However, the proportion of fucose and galactose residues differed. These data suggest that the analysis of the fucose and the terminal galactose residue of such oligosaccharides will be useful in the classification of leukemia.
Kondo
A
A
Department of Applied Bioorganic Chemistry, Gifu University, Japan.
Hosokawa
Y
Y
Kiso
M
M
Hasegawa
A
A
Kato
I
I
D000117:Acetylglucosamine / Q000097:blood; D000588:Amines; D002240:Carbohydrate Sequence; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood*; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000097:blood*; D012016:Reference Values
D000588:Amines; D007074:Immunoglobulin G; D009844:Oligosaccharides; D000117:Acetylglucosamine