Determination of mirosamicin in animal tissues by high-performance liquid chromatography.
A simple and rapid method using high-performance liquid chromatography (HPLC) for the determination of mirosamicin in animal tissues has been developed. The drug was extracted with 0.3% metaphosphoric acid-methanol (7:3, v/v), and the extracts were cleaned on a Bond Elut SCX (500 mg) cartridge. The HPLC separation was performed on a Puresil 5C18 column (150 x 4.6 mm I.D.) with 0.05 M phosphate buffer (pH 2.5)-acetonitrile (70:30) as the mobile phase at a flow-rate of 0.5 ml/min; the drug was detected at 230 nm with 0.04 AUFS. The calibration graph was linear from 5 to 100 ng. The recoveries of microsamicin from various animal tissues fortified at 1.0 microgram/g were 83.7-88.6% with a relative standard deviation (R.S.D.) of 2.0-5.7%. The detection limit was 0.05 microgram/g.
Horie
M
M
Saitama Prefectural Institute of Public Health, Japan.
Saito
K
K
Nose
N
N
Oka
H
H
Nakazawa
H
H
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000494:pharmacology; D001681:Biological Assay; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D018942:Macrolides; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000187:drug effects; D013552:Swine
D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D018942:Macrolides; C026686:mycinamicins; C063027:mirosamicin