cDNA cloning of human kidney pyruvate carboxylase.
cDNA clones covering a total of 4017 bp were isolated corresponding to the full length of the message for human pyruvate carboxylase. The coding sequence consisted of a stretch of 3534 bp flanked by a 5' untranslated sequence of 82 bp and a 3' untranslated region of 389 bp excluding the poly-A tail. The translated amino acid sequence of 1178 residues contained consensus sequences for the covalent attachment for biotin, the binding of ATP and the binding of the substrate, pyruvate.
MacKay
N
N
Department of Biochemistry, University of Toronto, Ontario, Canada.
Rigat
B
B
Douglas
C
C
Chen
H S
HS
Robinson
B H
BH
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011766:Pyruvate Carboxylase / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D011766:Pyruvate Carboxylase