Immunoreactive GnRH suggesting a third form of GnRH in addition to cIIGnRH and sGnRH in the brain and pituitary gland of Prochilodus lineatus (Characiformes).
Molecular variants of GnRH (gonadotropin-releasing hormone) in brain and pituitary extracts of the South American characiforme Prochilodus lineatus were studied using a combination of reverse-phase high-performance liquid chromatography and radioimmunoassay with different antisera. In brain extracts our study revealed that this fish has at least two different types of GnRH: cIIGnRH (chicken II) and sGnRH (salmon), and possibly a third variant of this molecule. In pituitary extracts we could find only two immunoreactive peaks corresponding to sGnRH and the possible third form.
Somoza
G M
GM
Departamento de Biotechnología Acuática, Fundación Centro de Investigaciones Médicas Albert Einstein, Buenos Aires, Argentina.
Stéfano
A
A
D'Eramo
J L
JL
Canosa
L F
LF
Fridman
O
O
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D005399:Fishes / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000032:analysis*; D007106:Immune Sera; D010902:Pituitary Gland / Q000737:chemistry*; D011863:Radioimmunoassay
D007106:Immune Sera; D007987:Gonadotropin-Releasing Hormone; C047717:LHRH, His(5)-Trp(7)-Tyr(8)-