A new bullous pemphigoid antigen.
Previously we reported a case of subepidermal blistering disease, which resembled bullous pemphigoid clinically. An immunoblot analysis revealed that the patient's serum reacted exclusively with a 450-kD epidermal polypeptide. The 450-kD antigen was also expressed in human keratinocytes and a human squamous carcinoma (HO 1-u-1 or A431) cell line. Using the patient's serum, cDNA clones in a bacteriophage lambda gt11 library derived from human foreskin keratinocytes were immune-screened. One positive clone was isolated from 2 million clones. Northern blot analysis showed that the inserted part of this clone hybridized with a more than 12-kb mRNA species derived from HO 1-u-1 cells. The deduced amino acid sequence had no homology with any other human component.
Fujiwara
S
S
Department of Dermatology, Oita Medical University, Japan.
Kohno
K
K
Iwamatsu
A
A
Shinkai
H
H
D001324:Autoantigens / Q000032:analysis*; D015152:Blotting, Northern; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D002352:Carrier Proteins; D003001:Cloning, Molecular; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D006801:Humans; D015603:Keratinocytes / Q000276:immunology; D008970:Molecular Weight; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D010391:Pemphigoid, Bullous / Q000276:immunology*; D012867:Skin / Q000276:immunology; D012878:Skin Neoplasms / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology; D000097805:Collagen Type XVII
D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII; C486268:DST protein, human