Comparative studies on tryptophan binding to hepatic nuclear envelopes in Sprague-Dawley and Lewis rats.
Since Lewis rats are susceptible to many inflammatory diseases and have been used in an experimental model of the eosinophilia-myalgia syndrome, we investigated whether Lewis rats would respond to L-tryptophan as have Sprague-Dawley rats reported earlier. In this comparative study using females of both strains, we observed a decrease in the affinity of in vitro L-tryptophan binding to hepatic nuclei and nuclear envelopes of Lewis rats compared with Sprague-Dawley rats. However, in vivo stimulatory effects of administering L-tryptophan on hepatic polyribosomal aggregation, protein synthesis, and nuclear RNA release were similar in both strains. In vitro [3H]tryptophan binding to hepatic nuclear envelopes, using L-tryptophan implicated in cases of the eosinophilia-myalgia syndrome, revealed less specific binding than when using nonimplicated L-tryptophan in both strains. The possible significance of the quantitative difference in the binding affinity of L-tryptophan to hepatic nuclei of Lewis rats compared with those of Sprague-Dawley rats is as yet undetermined.
Sidransky
H
H
Department of Pathology, George Washington University Medical Center, Washington, District of Columbia 20037.
Verney
E
E
D000818:Animals; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000378:metabolism; D004339:Drug Compounding; D016603:Eosinophilia-Myalgia Syndrome / Q000139:chemically induced; D005215:Fasting; D005260:Female; D007564:Japan; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew / Q000378:metabolism*; D017207:Rats, Sprague-Dawley / Q000378:metabolism*; D014364:Tryptophan / Q000009:adverse effects / Q000378:metabolism* / Q000494:pharmacology; D014366:Tryptophan Oxygenase / Q000378:metabolism
D014364:Tryptophan; D014366:Tryptophan Oxygenase