Simple method of purification of Escherichia coli heat-labile enterotoxin and cholera toxin using immobilized galactose.
A simple method for purification of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli and for purification of cholera toxin using immobilized D-galactose column is described. A single run of column chromatography yielded homogeneous toxin as demonstrated by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis.
Uesaka
Y
Y
Institute for Diagnostic Reagents, Nissui Pharmaceutical Co., Ibaraki, Japan.
Otsuka
Y
Y
Lin
Z
Z
Yamasaki
S
S
Yamaoka
J
J
Kurazono
H
H
Takeda
Y
Y
D001427:Bacterial Toxins / Q000302:isolation & purification*; D002772:Cholera Toxin / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D004768:Enterotoxins / Q000302:isolation & purification*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005690:Galactose
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli; D005690:Galactose