Interaction of basic fibroblast growth factor (FGF-2) with nonresponsive HeLa cells.
Human HeLa adenocarcinoma cells did not respond to basic fibroblast growth factor (bFGF or FGF-2) but did bind the same amount of bFGF as responsive Chinese hamster lung fibroblasts (CCL 39). Heparinase II treatment of HeLa and CCL 39 cells resulted in a decrease of bFGF binding by 96 and 57%, respectively, indicating that heparan sulfate molecules were involved in bFGF binding. On HeLa cells, bFGF bound to a single family of low-affinity sites. Cross-linking experiments of 125I-bFGF to HeLa cells yielded several labeled complexes. Cell-associated 125I-bFGF was internalized in both cell types either by high-affinity receptors and heparitinase-sensitive sites in CCL 39 cells or by heparitinase-sensitive binding sites only in HeLa cells. The binding of bFGF to nonresponsive HeLa cells and its internalization via a family of heparitinase-sensitive binding sites might illustrate other functions of bFGF unrelated to cell proliferation.
Gannoun-Zaki
L
L
Laboratoire C.R.R.E.T., INSERM CJF 9014, Université Paris XII, Créteil, France.
Pieri
I
I
Badet
J
J
Barritault
D
D
D000818:Animals; D001665:Binding Sites; D015152:Blotting, Northern; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002704:Chlorates / Q000494:pharmacology; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006367:HeLa Cells / Q000166:cytology / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000166:cytology / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
D002704:Chlorates; D004247:DNA; D016222:Fibroblast Growth Factor 2; D006497:Heparan Sulfate; D011133:Polysaccharide-Lyases; D011994:Recombinant Proteins; C021349:heparitinsulfate lyase; C032706:sodium chlorate