Lipoperoxidation in plasma and red blood cells of patients undergoing haemodialysis: vitamins A, E, and iron status.
In 14 patients undergoing haemodialysis, lipoperoxidation (LPO) processes were determined in plasma and red blood cells (RBC) before and after a dialysis session by determining (a) the direct substrate, polyunsaturated fatty acids (PUFA); (b) the end product of LPO, malondialdehyde (MDA); and (c) the hydrophobic antioxidant systems, vitamins A and E. In plasma before dialysis, linoleic and arachidonic acid, and the antioxidant vitamin E, were significantly lowered as compared to the healthy controls (p < 0.05). On the contrary, the free MDA level was enhanced (p < 0.05). These results were emphasized by a dialysis session. In RBC of these patients, no difference in linoleic acid, free MDA, or vitamin E level were observed before or after dialysis when compared to controls. However, only vitamin A was significantly higher in haemodialysis patients (before and after dialysis) and in renal failure patients (p < 0.05) than in the healthy control group. The present results suggest that increased RBC vitamin A may offer some degree of protection against oxidative stress in erythrocytes, but not in plasma where LPO is demonstrated.
Peuchant
E
E
Laboratoire de Biochimie Médicale A, Université de Bordeaux II 146, France.
Carbonneau
M A
MA
Dubourg
L
L
Thomas
M J
MJ
Perromat
A
A
Vallot
C
C
Clerc
M
M
D000328:Adult; D000368:Aged; D004912:Erythrocytes / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005260:Female; D005609:Free Radicals; D006801:Humans; D007501:Iron / Q000097:blood; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D010949:Plasma / Q000378:metabolism; D006435:Renal Dialysis / Q000009:adverse effects*; D014511:Uremia / Q000097:blood / Q000628:therapy; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood
D005231:Fatty Acids, Unsaturated; D005609:Free Radicals; D014801:Vitamin A; D014810:Vitamin E; D008315:Malondialdehyde; D007501:Iron