title: Simple method of purification of Escherichia coli heat-labile enterotoxin and cholera toxin using immobilized galactose.
abstract: A simple method for purification of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli and for purification of cholera toxin using immobilized D-galactose column is described. A single run of column chromatography yielded homogeneous toxin as demonstrated by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis.
authors.affiliation: Institute for Diagnostic Reagents, Nissui Pharmaceutical Co., Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Uesaka
authors.forename: Y
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Z
authors.initials: Z
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: Yamasaki
authors.forename: J
authors.initials: J
authors.lastname: Yamaoka
authors.forename: H
authors.initials: H
authors.lastname: Kurazono
authors.forename: Y
authors.initials: Y
authors.lastname: Takeda
mesh_terms: D001427:Bacterial Toxins / Q000302:isolation & purification*; D002772:Cholera Toxin / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D004768:Enterotoxins / Q000302:isolation & purification*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005690:Galactose
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli; D005690:Galactose