Cloning and sequence analysis of the gene encoding isocitrate lyase from Rhodococcus fascians.
An isocitrate lyase (Icl)-encoding gene (icl) from the Gram+ plant pathogen Rhodococcus fascians was identified serendipitously as part of a scrambled fragment after shotgun cloning in the promoter probe vector, pDP1. The Icl protein is 429 amino acids long (47.11 kDa) and has a predicted pI of 4.84; it is 54% similar to the Escherichia coli Icl and 24-27% to eukaryotic homologues. Comparison of the prokaryotic and eukaryotic Icl confirms the earlier proposal of Matsuoka and McFadden [J. Bacteriol. 143 (1988) 4528-4536] that the enzyme has enlarged during evolution.
Vereecke
D
D
Laboratorium voor Genetica, Universiteit Gent, Belgium.
Villarroel
R
R
Van Montagu
M
M
Desomer
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D007522:Isocitrate Lyase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012240:Rhodococcus / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D007522:Isocitrate Lyase