title: Immunocytochemical identification of androgen receptor in mouse osteoclast-like multinucleated cells.
abstract: Expression of androgen receptor (AR) in mouse osteoclast-like multi-nucleated cells (OCs) was examined with immunocytochemical techniques. Murine OCs were obtained by co-culturing mouse osteoblastic cells and bone marrow cells. Three preparations of polyclonal anti-AR antibody which were raised in rabbit against different parts of the human AR were employed for the experiments. Specific staining for AR was demonstrated in the nuclei and the perinuclear area of mouse OCs. This is the first report demonstrating the presence of AR in osteoclast-like cells.
authors.affiliation: Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mizuno
authors.forename: T
authors.initials: T
authors.lastname: Hosoi
authors.forename: S
authors.initials: S
authors.lastname: Inoue
authors.forename: A
authors.initials: A
authors.lastname: Ikegami
authors.forename: M
authors.initials: M
authors.lastname: Kaneki
authors.forename: Y
authors.initials: Y
authors.lastname: Akedo
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Ouchi
authors.forename: C
authors.initials: C
authors.lastname: Chang
authors.forename: H
authors.initials: H
authors.lastname: Orimo
mesh_terms: D000818:Animals; D000906:Antibodies; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D010010:Osteoclasts / Q000737:chemistry* / Q000166:cytology; D011944:Receptors, Androgen / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D011944:Receptors, Androgen