Induction of a calbindin-D9K-like protein in avian muscle cells by 1,25-dihydroxy-vitamin D3.
1,25-dihydroxy-vitamin D3 induces the synthesis of a 9 kDa calcium binding protein in chick embryo myoblasts. This work revealed comigration of the myoblast protein with rat calbindin-D9K in SDS-polyacrylamide gels. Western-blot analysis with a specific calbindin-D9K antibody showed the presence of an immunoreactive protein of 9 kDa in chick tissues. In addition, higher levels of myoblast mRNA hybridizing to a specific cDNA probe for rat calbindin-D9K were detected in response to 1,25-dihydroxy-vitamin D3. Northern hybridization analysis showed that the increase was related to a single 550 nucleotide mRNA species. These results provide the first evidence on the presence of calbindin-D9K or a closely related protein in avian tissues and its inducible expression in muscle by 1,25-dihydroxy-vitamin D3.
Lila
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D
Departamento de Biologia, Universidad Nacional de Sur, Bahia Blanca, Argentina.
Susana
Z
Z
Ricardo
B
B
D000818:Animals; D064026:Calbindins; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis*; D014158:Transcription, Genetic
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; C576508:S100g protein, rat; D002117:Calcitriol