Analysis of the C-polyhedrin genes from different geographical isolates of a type 5 cytoplasmic polyhedrosis virus.
The C-polyhedrin genes of two different geographic isolates of a type 5 cytoplasmic polyhedrosis virus (CPV) were cloned. A CPV infecting Orgyia pseudotsugata (OpCPV), isolated in the Pacific Northwest of the U.S.A., and a CPV infecting Heliothis armigera, isolated in South Africa, were studied. Both genes were found to be 883 nucleotides in length and encoded a predicted protein of 246 residues (M(r) of 28,890). Comparison of the nucleotide sequences of these two viruses with another type 5 geographic isolate, infecting Euxoa scandens (EsCPV; isolated in Eastern Canada), showed that there were only 17 nucleotide differences among the three genes. The only nucleotide variation that had an effect on the encoded protein was a deletion of nucleotide 774 in the gene of EsCPV. The deletion introduces a frameshift mutation resulting in the alteration of the carboxyl-terminal amino acid sequence. Sequence alignment of the OpCPV C-polyhedrin showed little homology to a type 1 CPV (infecting Bombyx mori) or with analogous proteins (N-polyhedrins) from two baculoviruses infecting O. pseudotsugata. Interestingly, most of the conserved residues between the N- and C-polyhedrins were either basic or aromatic amino acids.
Galinski
M S
MS
Department of Molecular Biology, Cleveland Clinic Foundation, Ohio 44195.
Yu
Y
Y
Heminway
B R
BR
Beaudreau
G S
GS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002170:Canada; D005814:Genes, Viral / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009036:Moths / Q000382:microbiology*; D015205:Northwestern United States; D000077183:Occlusion Body Matrix Proteins; D012087:Reoviridae / Q000145:classification / Q000235:genetics*; D012088:Reoviridae Infections / Q000382:microbiology*; D017386:Sequence Homology, Amino Acid; D013019:South Africa; D013045:Species Specificity; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*; D015678:Viral Structural Proteins
D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus