A complete cDNA sequence for core I protein subunit of human ubiquinol-cytochrome c reductase.
Core I protein is the largest subunit of ubiquinol-cytochrome c reductase. We have isolated a complete cDNA clone of 1575 bp encoding the precursor to this protein by screening a human fibroblast cDNA library. Nucleotide sequence comparison showed that the human core I protein cDNA is about 85% homologous with the reported bovine counter part but with a large difference in the length of coding region which consists of 480 amino acids in human and 362 amino acids in bovine. Human core I protein is presumed to contain a presequence of 34 amino acids. Amino acid sequence alignment study showed that the predicted human core I protein has a significant homology with other members of matrix processing peptidase (MPP) and processing enhancing protein (PEP) family.
Islam
M M
MM
Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.
Tanaka
M
M
Suzuki
H
H
Torii
K
K
Hattori
N
N
Ozawa
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics*; D014450:Electron Transport Complex III / Q000737:chemistry / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D015723:Gene Library; D005815:Genetic Code; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D015342:DNA Probes; D018076:DNA, Complementary; D010446:Peptide Fragments; D014450:Electron Transport Complex III