[Isolation of intracellular inhibitors of bacterial RNAses on a column with immobilized Bacillus intermedius RNAse].
Intracellular inhibitors of RNases from Bacillus amyloliquefaciens and Bac. intermedius were isolated using affinity chromatography on covalently immobilized RNase from Bac. intermedius. The inhibitor of RNase from Bac. amyloliquefaciens was isolated from cells of E. coli HB 101 and purified to homogeneity. Proteins with molecular weights of 70, 36 and 20 kD possessing an inhibitory activity were found in the extract obtained from frozen cells of Bac. intermedius.
Bannikova
G E
GE
Varlamov
V P
VP
D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004800:Enzymes, Immobilized; D008970:Molecular Weight; D012260:Ribonucleases / Q000037:antagonists & inhibitors* / Q000378:metabolism
D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; C057438:barstar protein, Bacillus amyloliquefaciens; D012260:Ribonucleases
Vydelenie vnutrikletochnykh ingibitorov RNKaz bakteriĭ na kolonke s immobilizovannoĭ RNKazoĭ Bacillus intermedius.