Immobilization methods for NMR studies of cellular metabolism--a practical guide.
Nuclear magnetic resonance (NMR) can be used in a nondestructive manner to study cellular metabolism in intact cell samples such as a suspension of cells. However, many different cell types require a well-regulated medium that includes a buffered pH, as well as a continuous supply of oxygen. A series of methods that have been used for the maintenance of the extracellular conditions involves the immobilization of cells, followed by perfusion of the immobilized cell sample. NMR studies can then be performed for extended periods of time, as well as under sterile conditions. We discuss methodology, with perfused erythrocytes and thymocytes as specific examples.
Lundberg
P
P
Department of Biochemistry, University of Sydney, New South Wales, Australia.
Roy
S
S
Kuchel
P W
PW
D000464:Alginates; D000818:Animals; D002477:Cells / Q000378:metabolism*; D003470:Culture Media; D004912:Erythrocytes / Q000378:metabolism; D005782:Gels; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008863:Microspheres; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D012685:Sepharose; D012756:Sheep; D013048:Specimen Handling / Q000379:methods*; D013601:T-Lymphocytes / Q000378:metabolism; D013696:Temperature
D000464:Alginates; D003470:Culture Media; D005782:Gels; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D012685:Sepharose