[The isolation and purification of porcine interferon-gamma].
Porcine immune interferon (IF-gamma) was generated by stimulation with bacterial lectins. Physicochemical analysis showed porcine IF-gamma to be a glycoprotein with an isoelectric point around 8.4-8.8 and an apparent molecular weight of 28000 to 30000. A purification process used in this work included sequential steps of precipitation with ammonium sulphate and chromatographic separation on GPG-glass, con A-Sepharose and biogel P-100. This purification process gave a preparation of IF-gamma with specific activity of around 7 x 10(4) units per mg of protein.
Karpov
A V
AV
Ivanenko
V K
VK
Kishko
Ia G
IaG
Spivak
N Ia
NIa
Dumanskiĭ
V D
VD
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel; D007371:Interferon-gamma / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008722:Methods; D008970:Molecular Weight; D013552:Swine
D007371:Interferon-gamma
Poluchenie i ochistka svinogo gamma-interferona.