title: Unusual immunocytochemical and ultrastructural features of synovial fluid cells in multicentric reticulohistiocytosis. A case report.
abstract: We present the first report in which cells in synovial fluid from a patient with multicentric reticulohistiocytosis (MRH) were studied by immunocytochemistry for correlation with routine light and electron microscopy. MRH cells stained predominantly for lymphocyte-related surface antigens and not for the monocyte marker LEU M3 (CD14). These findings suggest a lymphocytic origin of MRH cells and not a histiocytic origin, as previously suggested. In addition, large numbers of membrane-bound, electron-dense, secretory-type granules were found ultrastructurally in the cytoplasm of these cells.
authors.affiliation: Rheumatology-Immunology Center, Veterans Administration Medical Center, Philadelphia, Pennsylvania 19104.
authors.forename: S S
authors.initials: SS
authors.lastname: Samaan
authors.forename: H R
authors.initials: HR
authors.lastname: Schumacher
authors.forename: T
authors.initials: T
authors.lastname: Villanueva
authors.forename: R
authors.initials: R
authors.lastname: Levin
authors.forename: B F
authors.initials: BF
authors.lastname: Atkinson
mesh_terms: D000911:Antibodies, Monoclonal; D001707:Biopsy, Needle; D015616:Histiocytosis, Non-Langerhans-Cell / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D013582:Synovial Fluid / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal