title: The actions of estradiol and epidermal growth factor in endometrial and endometriotic stroma in vitro.
abstract: OBJECTIVE
  To compare the effects of E2 and epidermal growth factor (EGF) on DNA and protein synthesis in endometrial and endometriotic stromal cell culture.
  
  
  METHODS
  Measurement of DNA and protein synthesis in cell cultures by trichloroacetic acid precipitation of radioactively labeled macromolecules.
  
  
  METHODS
  Tissue samples collected in operating theaters were processed in the cell culture laboratory.
  
  
  METHODS
  Samples of endometrium and endometriosis were collected synchronously at laparotomy.
  
  
  METHODS
  Stromal cells were isolated from tissue samples. Cultures of endometrial and endometriotic stromal cells were treated with 0.272 ng/mL (1 nmol/L) E2 or 10 ng/mL (1.6 nmol/L) EGF.
  
  
  METHODS
  3H-Thymidine and 3H-leucine incorporation were used to assess DNA and protein synthesis respectively in cell cultures.
  
  
  RESULTS
  There was no response to 0.272 ng/mL (1 nmol/L) E2 with respect to DNA or protein synthesis in either endometrial or endometriotic stromal cells, whereas 10 ng/mL (1.6 nmol/L) EGF significantly stimulated DNA and protein synthesis in both cell types. The effect of EGF was significantly greater in endometrial stroma than in endometriotic stroma.
  
  
  CONCLUSIONS
  There are differences in the stimulation of 3H-thymidine incorporation by 10 ng/mL (1.6 nmol/L) EGF in endometrial and endometriotic stromal cells in vitro.
authors.affiliation: University of Southampton, Princess Anne Hospital, United Kingdom.
authors.forename: S J
authors.initials: SJ
authors.lastname: Mellor
authors.forename: E J
authors.initials: EJ
authors.lastname: Thomas
mesh_terms: D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007930:Leucine / Q000378:metabolism; D014176:Protein Biosynthesis; D012016:Reference Values; D017154:Stromal Cells / Q000187:drug effects* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D014316:Tritium; D004958:Estradiol; D004815:Epidermal Growth Factor; D004247:DNA; D007930:Leucine; D013936:Thymidine