Tat-binding protein 7 is a subunit of the 26S protease.
Subunit 6 (S6), an integral component of the 26S protease from human erythrocytes, has been studied by SDS-PAGE, peptide mapping and sequence analysis. S6 was cleaved with CNBr and three internal peptides were sequenced. A comparison with known proteins in Genbank revealed that all three S6 peptides match the predicted sequence of TBP7, Tat-binding protein 7. Based on peptide matches covering more than 10% of the TBP7 sequence, and the fact that the migration of S6 on SDS-PAGE is consistent with the estimated molecular mass for TBP7, we conclude that subunit 6 of the 26S protease is TBP7.
Dubiel
W
W
Institut für Biochemie, Medizinische Fakultät (Charité), Humboldt-Universität, Berlin, Germany.
Ferrell
K
K
Rechsteiner
M
M
D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D003488:Cyanogen Bromide; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000737:chemistry*; D010449:Peptide Mapping; D046988:Proteasome Endopeptidase Complex; D014157:Transcription Factors / Q000737:chemistry* / Q000302:isolation & purification
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D010447:Peptide Hydrolases; C088863:PSMC4 protein, human; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; D000251:Adenosine Triphosphatases; D000074183:ATPases Associated with Diverse Cellular Activities; D003488:Cyanogen Bromide