A Clostridium perfringens vector for the selection of promoters.
A promoter selection vector for Clostridium perfringens genes was constructed from a C. perfringens-Escherichia coli shuttle vector, pJIR418. The plasmid carries a promoterless chloramphenicol acetyltransferase gene (catP), derived from pIP401, downstream of the multiple cloning sites of pUC18. When a promoter region of the phospholipase C gene was inserted into one of the cloning sites, derivatives of C. perfringens strain 13 carrying the resultant plasmid acquired resistance to chloramphenicol. This plasmid should be useful reporter system for C. perfringens genes.
Matsushita
C
C
Department of Microbiology, Kagawa Medical School, Japan.
Matsushita
O
O
Koyama
M
M
Okabe
A
A
D001483:Base Sequence; D015152:Blotting, Northern; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D003016:Clostridium perfringens / Q000235:genetics*; D017931:DNA Primers; D004926:Escherichia coli; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping
D017931:DNA Primers; D012333:RNA, Messenger; D015500:Chloramphenicol O-Acetyltransferase