title: Bone resorbing activity of surface-associated material from Actinobacillus actinomycetemcomitans and Eikenella corrodens.
abstract: The results of this study demonstrate that saline extracted surface-associated material (SAM) of Actinobacillus actinomycetemcomitans and Eikenella corrodens stimulates bone resorption at picomolar concentrations. Various inhibitors of known osteolytic mediators--indomethacin, interleukin-1 receptor antagonist (IL-1ra) and a neutralising antibody to murine tumour necrosis factor (TNF) alpha--were tested to determine the mechanism of action of these SAMs. Bone resorption induced by SAM from E. corrodens was slightly inhibited by indomethacin and almost completely inhibited by blocking the action of TNF alpha; that from A. actinomycetemcomitans was not significantly affected by either of these inhibitors.
authors.affiliation: Maxillofacial Surgery Research Unit, Eastman Dental Institute, University of London.
authors.forename: S
authors.initials: S
authors.lastname: Meghji
authors.forename: M
authors.initials: M
authors.lastname: Wilson
authors.forename: P
authors.initials: P
authors.lastname: Barber
authors.forename: B
authors.initials: B
authors.lastname: Henderson
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001853:Bone Marrow / Q000382:microbiology; D001862:Bone Resorption / Q000382:microbiology*; D002241:Carbohydrates / Q000032:analysis; D046508:Culture Techniques; D016124:Eikenella corrodens / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000032:analysis; D005615:Freezing; D007213:Indomethacin / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010010:Osteoclasts / Q000382:microbiology; D051381:Rats; D017208:Rats, Wistar; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors; D012886:Skull / Q000187:drug effects / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D004731:Endotoxins; D017472:Receptors, Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D007213:Indomethacin