Acute beneficial effect of Fluosol-DA on urinary excretion of stroma-free hemoglobin in the isolated rat kidney.
In a timed study over 75 min, divided into 5 15-min periods, experimental perfusions of isolated rat kidneys using Krebs-Ringer bicarbonate (KRB) containing 3.5% (uncrosslinked) stroma-free hemoglobin (SFH) plus 3.5% of the perfluorochemical Fluosol-DA were found to excrete only 13% as much total hemoglobin (Hb) as control perfusions using KRB containing 7% SFH alone (controls). The glomerular filtration rate was the same for experimentals and controls in 3 periods, and slightly higher in the experimentals in 2 periods. However, urine flow was found to be significantly decreased in all 5 periods for the experimentals relative to the controls. These effects can be explained by reabsorption of glomerular filtrate containing Hb by endocytosis of renal tubular cells.
Millis
R M
RM
Department of Physiology and Biophysics, College of Medicine, Howard University, Washington, DC 20059.
Barber
J D
JD
Anderson
W A
WA
Dehkordi
O
O
Toussaint
R M
RM
Ertugrul
L
L
D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D004338:Drug Combinations; D005466:Fluorocarbons / Q000494:pharmacology*; D005919:Glomerular Filtration Rate; D006454:Hemoglobins / Q000494:pharmacology*; D006895:Hydroxyethyl Starch Derivatives; D007668:Kidney / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014556:Urine
D001802:Blood Substitutes; D004338:Drug Combinations; D005466:Fluorocarbons; D006454:Hemoglobins; D006895:Hydroxyethyl Starch Derivatives; C016484:hemoglobin, stroma free; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination