Molecular cloning of sheep lung dipeptidase: a glycosyl phosphatidylinositol-anchored ectoenzyme that converts leukotriene D4 to leukotriene E4.
Sheep lung dipeptidase was released from a plasma membrane preparation by digestion with a phosphatidylinositol-specific phospholipase C. The dipeptidase was purified to homogeneity using affinity chromatography followed by high performance liquid chromatography. The NH2-terminal sequence was determined and employed to prepare a probe to clone the c-DNA of the enzyme. The primary structure of sheep lung dipeptidase, deduced from the c-DNA exhibited a high homology to kidney dipeptidases cloned from other animal species. Northern analysis detected the m-RNA of the dipeptidase in lung, kidney, and intestinal tissues of the sheep.
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S
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Department of Biochemistry, University of Missouri-Columbia.
Schmidt
F J
FJ
Campbell
B J
BJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004150:Dipeptidases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017998:Leukotriene D4 / Q000378:metabolism*; D017999:Leukotriene E4 / Q000378:metabolism*; D008168:Lung / Q000201:enzymology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D012756:Sheep; D010738:Type C Phospholipases
D018076:DNA, Complementary; D012333:RNA, Messenger; D017998:Leukotriene D4; D017999:Leukotriene E4; D010738:Type C Phospholipases; D004150:Dipeptidases; C038937:dipeptidase