Changes in the expression of decorin and biglycan in human articular cartilage with age and regulation by TGF-beta.
In chondrocytes isolated directly from human articular cartilage, without subsequent culture, biglycan mRNA levels decreased with the age of the donor, whereas those for decorin increased. In cultured chondrocytes in the presence of FCS, mRNA levels for biglycan remained similar to those in uncultured counterparts, while decorin transcription became depressed. The differential effect on decorin and biglycan mRNA expression was mimicked by the addition of TGF-beta. In the absence of exogenously added growth factors (TGF-beta or FCS) biglycan mRNA levels decreased, while those for decorin increased. In contrast, IGF-I showed no differential modulation of the relative abundance of the two messages. The opposite regulation of these two proteoglycans by TGF-beta was also reflected at the level of protein synthesis. It would appear that the need of articular cartilage for decorin is greatest in the adult, whereas the need for biglycan is greatest in the juvenile.
Roughley
P J
PJ
Genetics Unit, Shriners Hospital for Crippled Children, Department of Surgery, McGill University, Montreal, Canada.
Melching
L I
LI
Recklies
A D
AD
D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D058578:Biglycan; D002358:Cartilage, Articular / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002478:Cells, Cultured; D058575:Decorin; D016326:Extracellular Matrix Proteins; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007231:Infant, Newborn; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007700:Kinetics; D008875:Middle Aged; D011509:Proteoglycans / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D016212:Transforming Growth Factor beta / Q000494:pharmacology
C546884:BGN protein, human; D058578:Biglycan; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I