title: Immunohistochemical localization of metabotropic glutamate receptors, mGluR2 and mGluR3, in rat cerebellar cortex.
abstract: The distribution of the metabotropic glutamate receptors mGluR2 and mGluR3 was immunohistochemically examined in the rat cerebellar cortex at both light and electron microscope levels. An antibody was raised against a fusion protein containing a C-terminal portion of mGluR2. On immunoblot, the antibody reacted with both mGluR2 and mGluR3 in rat brain. mGluR2/3 immunoreactivity was expressed in cell bodies, dendrites, and axon terminals of Golgi cells, as well as in presumed glial processes. Golgi axon terminals with mGluR2/3 immunoreactivity were often encountered in the vicinity of glutamatergic mossy fiber terminals. The results suggest that transmitter glutamate may exert control influences upon Golgi cells not only through dendritic mGluR2/3, but also through axonal mGluR2/3.
authors.affiliation: Department of Morphological Brain Science, Faculty of Medicine, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ohishi
authors.forename: R
authors.initials: R
authors.lastname: Ogawa-Meguro
authors.forename: R
authors.initials: R
authors.lastname: Shigemoto
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: S
authors.initials: S
authors.lastname: Nakanishi
authors.forename: N
authors.initials: N
authors.lastname: Mizuno
mesh_terms: D000818:Animals; D001369:Axons / Q000737:chemistry; D002460:Cell Line; D002525:Cerebellar Cortex / Q000737:chemistry* / Q000648:ultrastructure; D003712:Dendrites / Q000737:chemistry; D005260:Female; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D011689:Purkinje Cells / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000032:analysis*; D014018:Tissue Distribution
chemical_list: D018094:Receptors, Metabotropic Glutamate