title: Cellular defense mechanisms in C. capitata: recognition and entrapment of E. coli by hemocytes.
abstract: The mechanism of recognition of foreignness and entrapment of invaders by the immune system of insects is unknown. In this report using hemocyte monolayer preparations and biochemical analysis we demonstrate the requirements for recognition of E. coli in vitro, their entrapment by hemocytes, and nodule formation. A model system consisting of an isolated hemocyte protein (47 KDa), isolated hemocyte tyrosinase, isolated hemocytes, tyrosine, and E. coli was used to obtain these results. The 47 kDa polypeptide has the ability to form adducts with tyrosine derivatives generated by the action of tyrosinase and to attach to the E. coli surface. The latter process takes place independently of tyrosinase activity. When the E. coli-47KDa protein complex was overlaid on hemocyte monolayers followed by tyrosine and tyrosinase or vice versa, the bacteria were entrapped by hemocytes. The same results were obtained when the monolayers were overlaid with 47 KDa protein, followed by E. coli-47 KDa protein complex and then tyrosine and tyrosinase. The same experimental procedure in test tubes resulted in nodule formation. These results permit us to propose that the most likely explanation for the entrapment of E. coli to hemocytes and the formation of nodules is the production of E. coli-47 KDa complexes, and their crosslinking through a quinone intermediate generated by the action of tyrosinase on hemocytes.
authors.affiliation: Department of Biology, University of Patras, Greece.
authors.forename: V J
authors.initials: VJ
authors.lastname: Marmaras
authors.forename: N D
authors.initials: ND
authors.lastname: Charalambidis
authors.forename: M
authors.initials: M
authors.lastname: Lambropoulou
mesh_terms: D000818:Animals; D004175:Diptera / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D006434:Hemocytes / Q000276:immunology*; D014442:Monophenol Monooxygenase / Q000378:metabolism
chemical_list: D014442:Monophenol Monooxygenase