title: Spermatogenic and mutagenic damage after paternal exposure to systemic indium-114m.
abstract: The cytotoxic and mutagenic consequences of systemic administration of 114mIn have been examined. Adult male rats were dosed intraperitoneally with 14.8 or 3.7 MBq/kg 114mIn. Approximately 0.25% of the injected radioactivity was localized within the testis by 24 h and was retained with an effective half-life of 49.5 days. Breeding studies were started 3 days after injection, males being housed with two females for seven consecutive mating trials of 19 days, separated by 2 days. Indium-114m caused a reduction in litter size and an increase in the incidence of pre- and postimplantation losses and dominant lethal mutations. These effects became evident from 24 days but were most marked between 87-126 days after treatment and persisted up to 147 days. When animals were mated 200 days after treatment, no significant changes were observed. In a parallel study, administration of 14.8 MBq/kg 114mIn resulted in decreased testis and epididymal weight and sperm reserves. Maximal reduction occurred between 87-108 days after injection followed by recovery toward control values, but neither organ had reached normal levels at 200 days. A single dose of 3.7 MBq/kg, however, had no effect on reproductive organ weight or sperm content. Male F1 progeny from the 14.8 MBq/kg group of the second mating period (commencing at 24 days) displayed decreased testis weights and sperm content and provoked a higher incidence of dominant lethal mutations. This effect was not observed in male progeny from any other time or the alternative dose level.
authors.affiliation: Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, United Kingdom.
authors.forename: K P
authors.initials: KP
authors.lastname: Hoyes
authors.forename: H L
authors.initials: HL
authors.lastname: Sharma
authors.forename: H
authors.initials: H
authors.lastname: Jackson
authors.forename: J H
authors.initials: JH
authors.lastname: Hendry
authors.forename: I D
authors.initials: ID
authors.lastname: Morris
mesh_terms: D000818:Animals; D001835:Body Weight / Q000528:radiation effects; D007205:Indium Radioisotopes / Q000009:adverse effects; D007248:Infertility, Male / Q000209:etiology; D008098:Litter Size; D008297:Male; D009154:Mutation; D009929:Organ Size / Q000528:radiation effects; D011829:Radiation Dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis / Q000528:radiation effects*; D013737:Testis / Q000528:radiation effects
chemical_list: D007205:Indium Radioisotopes