Human interleukin for DA cells or leukemia inhibitory factor is released by Vero cells in human embryo coculture.
In the light of the newly discovered implications of human interleukin for DA cells and leukemia inhibitory factor in embryology, we searched for the presence of this soluble cytokine in the supernatant of Vero cell coculture systems. Using a bioassay as well as a specific ELISA, we demonstrated that Vero cells are able to release large quantities of human interleukin for DA cells and leukemia inhibitory factor in the embryo-growing medium of such cocultures.
Papaxanthos-Roche
A
A
Department of Reproductive Biology, Maternité Pellegrin, CHU de Bordeaux, France.
Taupin
J L
JL
Mayer
G
G
Daniel
J Y
JY
Moreau
J F
JF
D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003470:Culture Media / Q000378:metabolism; D003584:Cytological Techniques; D004622:Embryo, Mammalian / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006131:Growth Inhibitors / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000378:metabolism*; D008297:Male; D051379:Mice; D014709:Vero Cells / Q000378:metabolism*
D003470:Culture Media; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines