title: Preparative scale culture of Escherichia coli cells expressing the human immunodeficiency virus type 1 Tat protein.
abstract: A procedure leading to a 100-liter fermentor culture of Escherichia coli cells expressing the human immunodeficiency virus type 1 (HIV-1) trans-activator (Tat) protein is described. The effects of growth temperature and of cell density at the time of induction on the yield of Tat were investigated. Tat was identified by SDS-gel electrophoresis and Western blot. Tat represents approximately 10% of the soluble protein in the cell lysate.
authors.affiliation: Molecular Biology Institute, University of California at Los Angeles 90024.
authors.forename: M C
authors.initials: MC
authors.lastname: McKenna
authors.forename: C
authors.initials: C
authors.lastname: Muchardt
authors.forename: R
authors.initials: R
authors.lastname: Gaynor
authors.forename: D
authors.initials: D
authors.lastname: Eisenberg
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005285:Fermentation; D015696:Gene Products, tat / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D005813:Genes, Synthetic; D015756:Genes, tat; D015497:HIV-1 / Q000235:genetics*; D007218:Industrial Microbiology / Q000379:methods*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus