DNA profiling of Bursaphelenchus species.
A 1190-bp DNA fragment, designated X14, was isolated from a Bursaphelenchus xylophilus (isolate J10) genomic library. Used as a probe for DNA profiling, this fragment identifies the pinewood nematode (Bursaphelenchus spp.) at both the species and isolate level. The DNA profiles of a number of different species and isolates hybridised with the X14 probe, are presented. The nucleotide sequence of the 1190-bp probe was determined. Secondary structure analysis identified a large imperfect inverted repeat (IR) element within the probe which could form a large stem-loop structure in the RNA.
Harmey
J H
JH
Department of Botany, University College Dublin, Belfield, Ireland.
Harmey
M A
MA
D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D015698:Genomic Library; D008969:Molecular Sequence Data; D009348:Nematoda / Q000145:classification* / Q000235:genetics*; D009349:Nematode Infections / Q000453:epidemiology; D010935:Plant Diseases / Q000469:parasitology; D013045:Species Specificity; D014197:Trees / Q000469:parasitology
D015342:DNA Probes