Resolution of murine chlamydial genital infection by the adoptive transfer of a biovar-specific, Th1 lymphocyte clone.
MoPn-specific T-cell clones were isolated from a T-cell line that was capable of curing chlamydial genital infection by the Chlamydia trachomatis agent of mouse pneumonitis (MoPn) after adoptive transfer. Two clones (designated as 2.14-0 and 2.14-3) were characterized by flow cytometry techniques to be homogenous for L3T4, CD3, and alpha/beta T cell receptor (TcR) T-helper cell markers. The two clones were biovar specific, because they reacted to MoPn but not the Chlamydia psittaci agent of guinea pig inclusion conjunctivitis (GPIC) or C. trachomatis, serovar type E. Cytokine profile analysis, by a combination of bioassays, ELISA, and slot/Northern blotting for specific cytokine messenger RNAs, further revealed that cultures of antigen-stimulated clone 2.14-0 contained interleukin-2 (IL-2), tumor necrosis factor-alpha, and gamma interferon (a T helper 1 cell [Th1] profile). Clone 2.14-3 was also positive for gamma interferon, a level much lower than that of clone 2.14-0, and negative for IL-4 secretion, suggesting a Th1 profile as well. The ability of these clones to bring about the resolution of the chronic genital chlamydial infection of nude mice was tested by the adoptive transfer of 10(7) cells of each clone into the mice. By 4 weeks after cell transfer of clone 2.14-0, 81% of recipient nude mice (30 of 37) resolved the disease. In contrast, clone 2.14-3 or a control T-cell clone specific for a heterologous antigen were unable to resolve the infection in 20 recipients in each case, even after 100 days.(ABSTRACT TRUNCATED AT 250 WORDS)
Igietseme
J U
JU
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205.
Ramsey
K H
KH
Magee
D M
DM
Williams
D M
DM
Kincy
T J
TJ
Rank
R G
RG
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000276:immunology / Q000628:therapy*; D002692:Chlamydia trachomatis / Q000276:immunology; D002908:Chronic Disease; D002999:Clone Cells; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005831:Genital Diseases, Female / Q000276:immunology / Q000628:therapy*; D016130:Immunophenotyping; D016219:Immunotherapy, Adoptive; D008213:Lymphocyte Activation; D051379:Mice; D008819:Mice, Nude; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000637:transplantation*
D000907:Antibodies, Bacterial; D017252:CD3 Complex; D016207:Cytokines; D016693:Receptors, Antigen, T-Cell, alpha-beta