[Lack of foto reactivation of the Haemophilus influenzae transforming DNA mutated with near ultraviolet light (325-400nm)].
Three extracts from Saccharomyces cerevisiae were obtained by salting out with ammonium sulfate, these were I-G, EFRL-II-G and III-G. Fraction EFRL-II-G showed the highest photoreactivating activity on DNA str2000 irradiated with far UV light. However, the same fraction did not reactivate DNA str2000 previously inactivated by near UV irradiation. We think that the inactivation by near-UV was not due to photochemically-formed pyrimidine dimers. Decrease in spontaneous mutation frequency of cells transformed with DNA str2000 irradiated with near-UV light, was the same with the DNA treated with active or heat inactivated EFRL-II-G, therefore we may conclude that DNA lesions responsible for the effect are difference to pyrimidine dimers.
Alarcón-Hernández
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Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, México, D.F.
Cabrera-Juárez
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D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000302:isolation & purification / Q000378:metabolism; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D006193:Haemophilus influenzae / Q000235:genetics* / Q000528:radiation effects; D016296:Mutagenesis; D011740:Pyrimidine Dimers / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014169:Transformation, Bacterial / Q000528:radiation effects*; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D005656:Fungal Proteins; D011740:Pyrimidine Dimers; D004255:Deoxyribodipyrimidine Photo-Lyase
Ausencia de fotorreactivación en el DNA transformante de Haemophilus influenzae, mutado con luz de ultravioleta cercano (325-400 nm).