title: Determination of OPC-17116, a new fluoroquinolone, in human alveolar macrophages and other biological matrices by high performance liquid chromatography (HPLC).
abstract: A sensitive and reproducible high performance liquid chromatography (HPLC) assay was developed for the determination of cell-associated levels of the new fluoroquinolone antimicrobial agent, OPC-17116 (Otsuka Pharmaceutical Co. Ltd.). The assay consisted of a reverse phase C18 analytical column with fluorescence detection at 288 nm and was used to measure levels of OPC-17116 in the constituents of bronchoalveolar lavage (epithelial lining fluid and alveolar macrophages). Thus it was possible to measure drug concentration at specific sites in the respiratory tree. This is especially important because of differences that may exist between these levels and those found in serum. In addition, a good correlation was found between the HPLC assay and an established microbiological assay.
authors.affiliation: Department of Microbiology, Dudley Road Hospital, Birmingham, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Woodcock
authors.forename: J M
authors.initials: JM
authors.lastname: Andrews
authors.forename: D
authors.initials: D
authors.lastname: Honeybourne
authors.forename: R
authors.initials: R
authors.lastname: Wise
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024841:Fluoroquinolones; D006801:Humans; D016676:Macrophages, Alveolar / Q000737:chemistry*; D010879:Piperazines / Q000032:analysis* / Q000097:blood; D015363:Quinolones / Q000032:analysis* / Q000097:blood
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D010879:Piperazines; D015363:Quinolones; C075375:grepafloxacin