Study on measurement of delta-aminolevulinic acid in plasma by high-performance liquid chromatography.
A method for the determination of delta-aminolevulinic acid in plasma of lead-exposed workers by high-performance liquid chromatography with fluorescence detection of a fluorescent delta-aminolevulinic acid derivative (2-methylidineamino-3,5-diacetyl-4,6-dimethylpropionic acid) was established. The detection limit of delta-aminolevulinic acid in plasma was 0.01 microgram/ml at a signal-to-noise ratio of 5:1. A linear correlation was obtained between the amounts of delta-aminolevulinic acid injected from 0.01 to 0.5 microgram/ml (r = 0.999). The recovery of 0.05 and 0.1 microgram/ml of delta-aminolevulinic acid added to plasma with various concentrations of delta-aminolevulinic acid in plasma ranged from 80.0 to 100.8%. This method, combined with the use of an automatic sampler, should facilitate the routine measurement of delta-aminolevulinic acid in plasma.
Miyajima
K
K
Department of Occupational Health, Osaka Prefectural Institute of Public Health, Japan.
Hirata
M
M
Yoshida
T
T
Kosaka
H
H
Okayama
A
A
D000622:Aminolevulinic Acid / Q000097:blood*; D001800:Blood Specimen Collection; D002851:Chromatography, High Pressure Liquid; D005898:Glass; D006801:Humans; D007855:Lead Poisoning / Q000097:blood; D008669:Metallurgy; D009784:Occupational Diseases / Q000097:blood / Q000139:chemically induced; D013050:Spectrometry, Fluorescence
D000622:Aminolevulinic Acid