title: Crystallization and preliminary X-ray crystallographic analysis of phospholipid transfer protein from maize seedlings.
abstract: Phospholipid transfer protein from maize seedlings has been crystallized using trisodium citrate as precipitant. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions of a = 24.46 A, b = 49.97 A, and c = 69.99 A. The presence of one molecule in the asymmetric unit gives a crystal volume per protein mass (Vm) of 2.36 A3/Da and a solvent content of 48% by volume. The X-ray diffraction pattern extends at least to 1.6 A Bragg spacing when exposed to both CuK alpha and synchrotoron X-rays. A set of X-ray data to approximately 1.9 A Bragg spacing has been collected from a native crystal.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Shin
authors.forename: K Y
authors.initials: KY
authors.lastname: Hwang
authors.forename: K K
authors.initials: KK
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: R M
authors.initials: RM
authors.lastname: Sweet
authors.forename: S W
authors.initials: SW
authors.lastname: Suh
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D049294:Phospholipid Transfer Proteins; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D010940:Plant Proteins