Isolation of a human kappa opioid receptor cDNA from placenta.
By using a mouse delta opioid receptor cDNA as a probe, a kappa opioid receptor gene was isolated from a human genomic library. Reverse transcriptase/PCR was subsequently used to isolate the corresponding cDNA from human placenta mRNA. Characterization of the cloned receptor with a kappa agonist in transfected COS-7 cells, revealed a Kd of 0.67 nM, which is similar to rodent kappa 1 receptors. In competition binding experiments, non-kappa agonists had kis > 1000nM. Furthermore, when expressed in COS-7 cells, the kappa receptor is negatively linked to cAMP. A Northern blot showed the presence of two transcripts, 6 and 7 kB in size, in both placenta and brain mRNA, which hybridized to the kappa cDNA.
Mansson
E
E
Ohmeda PPD, Murray Hill, NJ 07974.
Bare
L
L
Yang
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D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D017473:Receptors, Opioid, kappa / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D017473:Receptors, Opioid, kappa