[Multiple genes of delta-endotoxins from Bacillus thuringiensis subspecies galleriae].
Cloning and expression in E. coli delta-endotoxin genes cryIAb7, cryIG and, cryIX from Bacillus thuringiensis ssp galleriae has been performed. Restriction mapping and partial sequencing have shown the identity of the 3'-terminal parts of cryIG and cryIX, although their 5'-terminal halves corresponding to the toxin are unique. Sequencing 5'-flanking region of cryIX revealed no translation initiation site, which indicates a nonfunctional state of the gene. The clusterized locating of the cryIG and cryIG genes has been shown. The absence of a promoter-like structure in the 5'-flanking region of cryIG suggests transcription of the gene as a bicistronic mRNA with cryIX. The extremely high homology of the cryIG and cryIX 3'-terminal parts (2 kB long) suggests a recombination act in the gene origin.
Shevelev
A B
AB
Karasin
A I
AI
Svarinskiĭ
M A
MA
Kadyrov
R M
RM
Kogan
Ia N
IaN
Chestukhina
G G
GG
Stepanov
V M
VM
D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D001483:Base Sequence; D003001:Cloning, Molecular; D004731:Endotoxins / Q000235:genetics*; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D009838:Oligodeoxyribonucleotides; C030956:insecticidal crystal protein, Bacillus Thuringiensis
Mnozhestvennye geny delta-éndotoksinov Bacillus thuringiensis podvida galleriae.