The crystallization and preliminary X-ray analysis of allosteric chorismate mutase.
An allosteric chorismate mutase, the Thr226-->Ile mutant, from the yeast Saccharomyces cerevisiae has been crystallized in space group P6(1)(P6(5)) using the hanging drop vapour diffusion method at room temperature. The cell dimensions are a = b = 95.8 A, c = 157.9 A, alpha = beta = 90 degrees, gamma = 120 degrees. It contains a dimer in the crystallographic asymmetric unit. The crystal diffracts to 2.2 A resolution. A native data set has been collected to 82% completeness at this resolution.
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Gibbs Chemical Laboratory, Harvard University, Cambridge, MA 02138.
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D000494:Allosteric Regulation; D002826:Chorismate Mutase / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D009154:Mutation / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D002826:Chorismate Mutase