Relationship between structure and function of neurons in the rat rostral nucleus tractus solitarii.
To investigate the relationship between the structure and function of neurons in the rostral (gustatory) nucleus tractus solitarii (rNTS), we analyzed the morphological and biophysical properties of rNTS neurons by performing whole-cell recordings in a brain slice preparation. Overall, neurons (n = 58) had a mean somal diameter of 16 microns, an average dendritic length of 598 microns, an average dendritic thickness of 0.91 microns, and a spine density of 0.037 spines/microns. Neurons were separated into three groups (elongate, multipolar, and ovoid) on the basis of previously established morphological criteria. The highest percentage (49%) of neurons were classified as ovoid, while 35% were multipolar and only 16% were elongate. The most frequently observed firing pattern, in all three cell types, elicited by a 1,200 ms, 100 pA depolarizing current pulse was a regularly firing spike train. However, the intrinsic firing properties of the remaining neurons were different. Thirty-one percent of the ovoid neurons responded with a short burst of action potentials and 44% of the elongate neurons showed a delay in the onset of the spike train following a hyperpolarizing prepulse. Less than 16% of the multipolar neurons demonstrated either of these firing characteristics. Therefore, rNTS neurons with similar morphology do not have unique biophysical properties. However, the data suggest that there may be subpopulations of the three morphological types, each of which displays a different firing pattern. Since the structure and function of the three morphological groups were not strictly correlated, these subpopulations may represent functional groups.
King
M S
MS
Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor 48109-1078.
Bradley
R M
RM
D000200:Action Potentials / Q000502:physiology; D000344:Afferent Pathways / Q000166:cytology / Q000502:physiology; D000818:Animals; D003712:Dendrites / Q000648:ultrastructure; D005260:Female; D066298:In Vitro Techniques; D008239:Lysine / Q000031:analogs & derivatives; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000166:cytology / Q000502:physiology*; D013649:Taste / Q000502:physiology
C013411:biocytin; D008239:Lysine