A sensitive spectrophotometry-based method for the determination of the rate of hydrogen peroxide generation in biological systems.
A new sensitive spectrophotometric method for the determination of the rate of hydrogen peroxide generation in biological systems has been developed. This method is based on the measurement of the oxidation rate of reduced cytochrome c by H2O2 in the presence of a mediator and permits the detection of H2O2 generation rates as low as 60 nM min-1. The solution of the differential equations of the kinetic process permitted the calculation of the kinetic rate constants and assessment of the conditions required to measure the hydrogen peroxide generation rate.
Di Paolo
M L
ML
Department of Biological Chemistry, University of Padova, Italy.
Scarpa
M
M
Rigo
A
A
D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D001681:Biological Assay; D002417:Cattle; D003574:Cytochrome c Group / Q000378:metabolism; D005470:Fluorometry; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000097:blood; D010648:Phenylacetates; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D013997:Time Factors
D003574:Cytochrome c Group; D010648:Phenylacetates; C008070:4-hydroxyphenylacetic acid; D006861:Hydrogen Peroxide; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors