title: Indoxyl-beta-D-glucuronide, the primary emitter of low-level chemiluminescence in plasma of hemodialysis patients.
abstract: Characteristic light emission induced by the oxidation of hydroxyl radicals has been found in plasma of hemodialysis patients (Agatsuma et al., Clin Chem 1992;38:48-55). We purified a primary emitter, a chemiluminescent component peaking at 430 nm, by anion-exchange chromatography and reversed-phase HPLC. By using proton nuclear magnetic resonance and authentic indoxyl compounds, we determined the primary emitter to be indoxyl-beta-D-glucuronide. Absorption and fluorescence spectra of the purified sample coincided well with those of authentic indoxyl-beta-D-glucuronide, as did the peak in the chemiluminescence emission spectrum. Retention time of the purified sample on reversed-phase HPLC, measured by fluorescence, was also in accordance with that of indoxyl-beta-D-glucuronide. To our knowledge, this is the first identification of a primary emitter of low-level chemiluminescence from a biological source.
authors.affiliation: Kohjinkai Hospital, Miyagi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Agatsuma
authors.forename: H
authors.initials: H
authors.lastname: Sekino
authors.forename: T
authors.initials: T
authors.lastname: Nagoshi
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005965:Glucuronates / Q000097:blood*; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D007211:Indoles / Q000097:blood*; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D007700:Kinetics; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D006435:Renal Dialysis; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013481:Superoxides / Q000378:metabolism
chemical_list: D005965:Glucuronates; D007211:Indoles; D013481:Superoxides; D017665:Hydroxyl Radical; C059836:indoxyl glucuronide