Seroepidemiological and clinical survey of feline immunodeficiency virus infection in northern Italy.
Four hundred and thirty-nine feline serum samples from cats with different living conditions in the north of Italy were tested for antibodies to feline immunodeficiency virus (FIV) and for antigen of Feline Leukemia Virus by enzyme-linked immunosorbent assay. A Western blot technique was also used on the positive sera in order to confirm the presence of specific antibodies to FIV. The Western blot enabled the detection of a false positive serum. The prevalence of FIV infection in this population was 12.5% and among the seropositive cats a greater proportion was male (74.5%) than female (25.5%). A correlation between the clinical status and the evolution of the pathology is described together with a score based on the severity of the stomatitis in infected cats. The Western blot patterns of positive samples were then compared with the stage of the pathology. Statistical analysis on the distribution of FIV in stray cats, cats with garden and courtyard access and strictly house-confined cats showed a highly significant risk of the infection in the first group.
Peri
E V
EV
Istituto di Microbiologia e Immunologia Veterinaria, Università degli Studi di Milano, Italy.
Ponti
W
W
Dall'ara
P
P
Rocchi
M
M
Zecconi
A
A
Bonizzi
L
L
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D015153:Blotting, Western / Q000662:veterinary; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005189:False Positive Reactions; D016181:Feline Acquired Immunodeficiency Syndrome / Q000453:epidemiology* / Q000276:immunology; D005260:Female; D016089:Immunodeficiency Virus, Feline / Q000276:immunology; D007558:Italy / Q000453:epidemiology; D008297:Male; D015995:Prevalence; D016036:Seroepidemiologic Studies
D000914:Antibodies, Viral