Comparison of liver microsome enzyme and fatty acid composition recovery in adult and old rats deficient in 18:3n-3 refed a diet containing 18:3n-3.
The effect of dietary n-3 deficiency on liver microsome enzymes activities and fatty acid composition was studied in adult (3 months old) and old rats (18 months old). At these two ages, deficient animals were refed with 18:3n-3 diet for 1 or 2 months and the recovery of these parameters was investigated. Cytochrome P 450 level was decreased by n-3 PUFA (Polyunsaturated fatty acid) deficiency. After refeeding, it returned to control values after 1 month. NADH-cytochrome b5 reductase activity was decreased, the activities of NADPH cytochrome c reductase, aminopyrine demethylase, aniline hydroxylase were also decreased, but in old rats they were increased by refeeding. N-3 PUFA deficiency caused a decrease of 18:2n-6 and 22:6n-3 and an increase in 20:4n-6, 22:5n-6 and 18:1n-9. After refeeding, in adult rats, the PUFA level remained lower; in old rats, the MUFA (Monounsaturated fatty acid) and PUFA levels returned to control values. Liver microsomal enzyme activities depend on the degree of unsaturation of fatty acids rather than the specific species of polyunsaturated fatty acids.
Dinh
L
L
Laboratoire de Neurotoxicologie, INSERM U26, Paris, France.
Dumont
O
O
Durand
G
G
D000375:Aging / Q000378:metabolism*; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005260:Female; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D005227:Fatty Acids; D015525:Fatty Acids, Omega-3