Cloning and expression of genes coding for carbohydrate degrading enzymes of Anaerocellum thermophilum in E.coli.
A gene library of Anaerocellum thermophilum Z-1320 was constructed in Escherichia coli using plasmid pUC18. Clones were selected by screening for hydrolysis of CMC, MU-Cel, lichenan and xylan. Recombinant clones expressing two beta-1,4-glucanases and two different xylanases were obtained. Both beta-1,4-glucanases were capable of degrading Avicel. Clones with xylanase activity also cleft PNPXyl, MU-Glu and MU-Cel. The genes of one beta-1,4-glucanase and one xylanase are probably located in the same region of A.thermophilum chromosome.
Bolshakova
E V
EV
Institute of Molecular Genetics, Russian Academy of Science, Moscow.
Ponomariev
A A
AA
Novikov
A A
AA
Svetlichnyi
V A
VA
Velikodvorskaya
G A
GA
D050260:Carbohydrate Metabolism; D002482:Cellulose / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005051:Eubacterium / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D044643:Glucan 1,4-beta-Glucosidase; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D006868:Hydrolysis; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D043325:Xylan Endo-1,3-beta-Xylosidase; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
D004269:DNA, Bacterial; D011994:Recombinant Proteins; D002482:Cellulose; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D043325:Xylan Endo-1,3-beta-Xylosidase; D044643:Glucan 1,4-beta-Glucosidase