Low-stringency PCR with diagnostically useful primers for identification of Leptospira serovars.
Primers proposed for the diagnosis of the pathogenic spirochete Leptospira spp. (C. Gravekamp, H. V. D. Kemp, M. Franzen, D. Carrington, G.J. Schoone, G.J.J.M. Van Eys, C. O. R. Everard, R.A. Hartskeel, and W.J. Terpstra, J. Gen. Microbiol. 139:1691-1700, 1993) have been found to produce complex serovar-specific patterns under low-stringency PCR conditions. Such patterns obtained by low-stringency PCR, which maintain the specific band as an internal control, offer, an approach to the standardized identification of Leptospira serovars in clinical laboratories.
de Caballero
O L
OL
Centro de Pesquisas René Rachou, FIOCRUZ, Minas Gerais, Brazil.
Dias Neto
E
E
Koury
M C
MC
Romanha
A J
AJ
Simpson
A J
AJ
D001431:Bacteriological Techniques / Q000592:standards / Q000706:statistics & numerical data; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D007919:Leptospira / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D007922:Leptospirosis / Q000175:diagnosis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012703:Serotyping
D017931:DNA Primers; D004269:DNA, Bacterial