A single Arabidopsis GF14 isoform possesses biochemical characteristics of diverse 14-3-3 homologues.
Arabidopsis cDNA clones of GF14 proteins originally were isolated on the basis of their association with the G-box DNA/protein complex by a monoclonal antibody screening approach. GF14 proteins are homologous to the 14-3-3 family of mammalian proteins. Here we demonstrate that recombinant GF14 omega, one member of the Arabidopsis GF14 protein family, is a dimeric protein that possesses many of the attributes of diverse mammalian 14-3-3 homologues. GF14 omega activates rat brain tryptophan hydroxylase and protein kinase C in a manner similar to the bovine 14-3-3 protein. It also activates exoenzyme S of Pseudomonas aeruginosa as does bovine brain factor activating exoenzyme S (FAS), which is itself a member of 14-3-3 proteins. In addition, GF14 omega binds calcium, as does the human 14-3-3 homologue reported to be a phospholipase A2. These results indicate that a single isoform of this plant protein family can have multiple functions and that individual GF14 isoforms may have multiple roles in mediating signal transductions in plants. However, GF14 omega does not regulate growth in an in vivo test for functional similarity to the yeast 14-3-3 homologue, BMH1. Thus, while a single plant GF14 isoform can exhibit many of the biochemical attributes of diverse mammalian 14-3-3 homologues, open questions remain regarding the physiological functions of GF14/14-3-3 proteins.
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Department of Horticulture, University of Florida, Gainesville 32611.
de Vetten
N C
NC
Sehnke
P C
PC
Isobe
T
T
Ichimura
T
T
Fu
H
H
van Heusden
G P
GP
Ferl
R J
RJ
D048948:14-3-3 Proteins; D000818:Animals; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins; D001921:Brain / Q000201:enzymology; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D003001:Cloning, Molecular; D004789:Enzyme Activation; D004926:Escherichia coli; D007700:Kinetics; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D014365:Tryptophan Hydroxylase / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D029681:Arabidopsis Proteins; D002135:Calcium-Binding Proteins; C087620:GF14 protein, Arabidopsis; D009419:Nerve Tissue Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D014446:Tyrosine 3-Monooxygenase; D014365:Tryptophan Hydroxylase; D011493:Protein Kinase C