[Analysis of fibroblast growth factor receptor genes expressed in the retinal pigment epithelium of chick embryos by reverse transcription polymerase chain reaction].
To characterize the mechanism by which retinal pigment epithelial cells (RPE) respond to fibroblast growth factors (FGFs), reverse transcription polymerase chain reaction (RT-PCR) was performed on 7.5-day chick embryonic RPE. We amplified and cloned tyrosine kinase related sequences from chick embryonic RPE messenger RNA and characterized the partial complementary DNA (cDNA) by nucleic acid sequencing. Three different FGF receptor (FGFR) genes were found to be expressed in 7.5-day chick embryonic RPE; two of them were cek 2 (FGFR3) and cek 3 (FGFR2), and the third one, named chick pigment epithelium derived FGFR (CPE-FGFR), has not been previously identified. The deduced amino acid sequence of CPE-FGFR is more than 70% identical with each previously characterized FGFR. It appears that the effect of FGFs on differentiation of RPE are mediated by several types of FGFRs.
Fujiwara
M
M
Department of Ophthalmology, Okayama University Medical School, Japan.
D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D015870:Gene Expression; D008969:Molecular Sequence Data; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor