title: Inhibition of CT-26 murine adenocarcinoma growth in the rectum of mice treated with recombinant human interleukin-6.
abstract: In the present study we evaluated the antitumor effects of recombinant human interleukin-6 (rhIL-6), expressed in Chinese hamster ovary cells, in a murine primary tumor model. We showed that treatment with rhIL-6 substantially inhibited the implantation and growth rates of CT-26 adenocarcinoma tumor cells in the rectal submucosa of syngeneic mice. This effect was achieved by injecting rhIL-6 for 7 consecutive days starting 1 day prior to tumor inoculation. No obvious antitumor effect was noted when rhIL-6 injections started 5 days after tumor inoculation. Analysis of the mechanisms by which rhIL-6 exerts its antitumor effects did not reveal a direct antitumor effect on CT-26 tumor cells or the up-regulation of major histocompatibility complex antigens on these cells. However, infiltration of lymphocytes at the tumor site was observed. Increase of carcinoembryonic antigen by IL-6 was clearly seen in human HT-29 colon carcinoma cells. The possible application of these results for adjuvant immunotherapy of selected colorectal patients and prevention of reimplantation of tumor cells disseminated during surgery is discussed.
authors.affiliation: Department of Oncology, Sourasky Medical Center, Ichilov Hospital, Tel Aviv, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Rabau
authors.forename: H
authors.initials: H
authors.lastname: Kashtan
authors.forename: S
authors.initials: S
authors.lastname: Baron
authors.forename: J
authors.initials: J
authors.lastname: Yossiphov
authors.forename: Y
authors.initials: Y
authors.lastname: Skornik
authors.forename: M
authors.initials: M
authors.lastname: Revel
authors.forename: A
authors.initials: A
authors.lastname: Eisenthal
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000627:therapeutic use; D012004:Rectal Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D015850:Interleukin-6; D011994:Recombinant Proteins