title: Post-transcriptional regulation of the Pseudomonas aeruginosa algC gene.
abstract: The algC gene (encoding phosphomannomutase) of Pseudomonas aeruginosa, similarly to the algD gene, is environmentally regulated through transcriptional activation of its promoter. This gene, like algD, has a long (244 bp) 5' untranslated leader region (5' UTR). Using transcriptional and translational algC::lacZ fusions, we show that even though the transcript levels are similar, the beta-galactosidase-specific activities of the translational fusions are much higher than those of the transcriptional fusions during the entire growth phase. Both the 5' UTR and the ribosomal-binding site are shown to be important for efficient translation of the algC mRNA.
authors.affiliation: Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago 60612.
authors.forename: S
authors.initials: S
authors.lastname: Fujiwara
authors.forename: A M
authors.initials: AM
authors.lastname: Chakrabarty
mesh_terms: D002237:Carbohydrate Dehydrogenases / Q000235:genetics; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D007763:Lac Operon; D017875:Phosphotransferases (Phosphomutases) / Q000235:genetics*; D014176:Protein Biosynthesis; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D014158:Transcription, Genetic
chemical_list: D002237:Carbohydrate Dehydrogenases; C020562:GDPmannose dehydrogenase; D017875:Phosphotransferases (Phosphomutases); C052799:phosphomannomutase