title: Genomic organization and chromosomal localization of the mouse synexin gene.
abstract: We have isolated and characterized the gene encoding mouse synexin, which consists of 14 exons and spans approximately 30 kbp of genomic DNA. The protein's unique N-terminal domain is encoded by six exons, and the C-terminal tetrad repeat, the site of the membrane-fusion and ion-channel domain, is encoded by seven exons. The first exon encodes the 5'-untranslated region. Analysis of synexin-gene expression in different mouse tissues shows that mRNA with exon 6 is only present in brain, heart and skeletal muscle. mRNA lacking exon 6 is expressed in all tissues we have examined. The initiation site for transcription was determined by primer-extension analysis and S1 nuclease mapping. Sequence analysis of the 1.3 kb 5'-flanking region revealed that the promoter has a TATA box located at position -25 and a number of potential promoter and regulatory elements. A CCAAT motif was not observed but CCATT is located in an appropriate position for the CCAAT motif upstream from the transcription-initiation start site. In addition, the 5'-flanking region contains two sets of palindromic sequences. Finally, we have determined that the functional synexin gene (Anx7) is located on mouse chromosome 14 and that a pseudogene (Anx7-ps1) is located on chromosome 10.
authors.affiliation: Laboratory of Cell Biology and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Zhang-Keck
authors.forename: M
authors.initials: M
authors.lastname: Srivastava
authors.forename: C A
authors.initials: CA
authors.lastname: Kozak
authors.forename: H
authors.initials: H
authors.lastname: Caohuy
authors.forename: A
authors.initials: A
authors.lastname: Shirvan
authors.forename: A L
authors.initials: AL
authors.lastname: Burns
authors.forename: H B
authors.initials: HB
authors.lastname: Pollard
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D017310:Annexin A7 / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001923:Brain Chemistry; D002874:Chromosome Mapping; D004247:DNA / Q000737:chemistry*; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping; D017421:Sequence Analysis; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014018:Tissue Distribution
chemical_list: D017310:Annexin A7; D012333:RNA, Messenger; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases