Alteration of ozone-induced airway permeability by oxygen metabolites and antioxidants.
This study determined the interactive effects of O3 and enzymatically-generated oxidants and antioxidants in the lung. Rats treated with dimethylthiourea (DMTU) or H2O2, generated by glucose/glucose oxidase, were exposed for 2 h to 0.6 or 0.8 ppm O3. A significant increase in the flux of total albumin in the bronchoalveolar lavage (BAL) and a concomitant elevation in the transport of 99mTc-diethylenetriaminepentaacetate (99mTc-DTPA) from trachea to blood occurred after O3 exposure. Pretreatment of rats with DMTU prevented the albumin flux in the BAL. Intratracheal instillation of glucose/glucose oxidase produced a localized response in trachea, but it did not affect the broncho-alveolar permeability. The results demonstrate an additive effect of O3 and an enzymatically-generated oxidant, and an antagonistic effect of an antioxidant in rats exposed to O3. The observations support the suggestion that a balance of oxidant-antioxidant system may be critical in maintaining respiratory integrity following O3 exposure.
Bhalla
D K
DK
University of California, Irvine 92717.
D000418:Albumins / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002463:Cell Membrane Permeability / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology; D005949:Glucose Oxidase / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D010100:Oxygen / Q000494:pharmacology*; D010126:Ozone / Q000037:antagonists & inhibitors / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344; D012137:Respiratory System / Q000187:drug effects* / Q000378:metabolism; D016284:Technetium Tc 99m Pentetate; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology
D000418:Albumins; D000975:Antioxidants; D010126:Ozone; C038983:1,3-dimethylthiourea; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D013890:Thiourea; D005947:Glucose; D010100:Oxygen; D016284:Technetium Tc 99m Pentetate