Asymmetrical DNA and AT/GC base content of differential sector of Pleurodeles waltl sexual bivalent: a quantitative fluorescence imaging analysis in lampbrush chromosomes.
The mitotic Z and W sex chromosomes in Pleurodeles seem to be identical. Earlier morphological and molecular analyses of lampbrush paired chromosomes in the female meiosis showed clearly that 20% of the chromosomal length located in the middle part of the sex bivalent (bivalent IV) is heteromorphic. We investigated here the base content and composition of the DNA axes in the heteromorphic region by quantitative fluorescence imaging using various base-specific (DAPI, Hoechst 33342 and chromo-mycin A3) or base-nonspecific (ethidium bromide) fluorescent DNA probes. Our results show a significantly higher percentage of AT bases in Z than in W differential sectors. In addition the entire base content of Z appears slightly higher than that of W.
Loones
M T
MT
Laboratoire de Génétique du Dévelopment, Université P. et M. Curie, Paris, France.
Amirand
C
C
Debey
P
P
Lacroix
J C
JC
Vigny
P
P
D000818:Animals; D001482:Base Composition; D004247:DNA / Q000737:chemistry*; D005260:Female; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D015335:Molecular Probes; D010999:Pleurodeles / Q000235:genetics*; D012730:Sex Chromosomes / Q000648:ultrastructure*
D005456:Fluorescent Dyes; D015335:Molecular Probes; D004247:DNA