High-performance liquid chromatographic determination of iophenoxic acid in serum.
Iophenoxic acid (IPA), a marker used to investigate the feeding behaviour of bait-consuming animals has previously been indirectly determined by measuring protein-bound iodine levels in serum or plasma. For the first time a method is reported for the direct determination of IPA in biological fluids. IPA was determined in de-proteinized serum by high-performance liquid chromatography (HPLC) on a C18 column with a mobile phase of acetonitrile-water. Isocratic and gradient systems are described with limits of detection of 0.2 microgram/ml (isocratic) and 0.05 microgram/ml (gradient). Recoveries from fox serum were 85% at 0.5 microgram/ml, 95% at 5 micrograms/ml and 91% at 50 micrograms/ml.
Jones
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Central Science Laboratory, Ministry of Agriculture, Fisheries and Food, Slough, UK.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005589:Foxes / Q000097:blood*; D006801:Humans; D007480:Iopanoic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
C008160:iophenoxic acid; D007480:Iopanoic Acid