title: Biochemical properties of recombinant human beta-glucuronidase synthesized in baby hamster kidney cells.
abstract: The cDNA sequence encoding human beta-glucuronidase [Oshima, Kyle, Miller, Hoffmann, Powell, Grubb, Sly, Troplak, Guise and Gravel (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 685-689] was expressed in baby hamster kidney (BHK) cells. After purification from the culture supernatant in one step by use of immunoaffinity chromatography, the biochemical properties of the enzyme were examined. With a pH optimum of 4.0, a Km of 1.3 mM and thermal stability up to 68 degrees C, this protein has characteristics very similar to those described for beta-glucuronidase from human placenta [Brot, Bell and Sly (1978) Biochemistry 17, 385-391. However, the recombinant product has several structural properties not previously reported for beta-glucuronidase isolated from natural sources. First, recombinant beta-glucuronidase is synthesized as a tetramer consisting of two disulphide-linked dimers. As can be inferred from the cDNA sequence, the enzyme possesses five cysteine residues after cleavage of the signal peptide. By introducing a C-terminal truncation, we eliminated the last cysteine at position 644. In the mutant, covalent linkage between two monomers is no longer observed, indicating that Cys-644 is involved in intermolecular disulphide-bond formation. The functional role of the disulphide bond remains elusive, as it was shown that (i) intracellular transport of the mutant is not impaired and (ii) it is still able to form an enzymically active tetramer. A second feature that has not previously been observed for beta-glucuronidase from any origin is the existence of two enzymically active species for recombinant beta-glucuronidase, when examined by gel filtration on a TSK 3000 column. With apparent molecular masses of 380 kDa and 190 kDa we propose that they represent tetramers and dimers respectively. Partial N-terminal sequencing and electrophoresis under denaturing conditions revealed that the dimers consist of subunits that have been proteolytically processed at their C-terminus losing 3-4 kDa in peptide mass. Controlled proteolysis demonstrates that the enzyme's overall protein backbone as well as its activity are resistant to a number of proteases. Only the C-terminal portion is susceptible to protease action, and the disulphide-linked form is readily converted into non-disulphide-bonded subunits. Pulse-chase analysis shows that human beta-glucuronidase remaining intracellular in BHK cells after synthesis undergoes a similar proteolytic processing event, i.e. a reduction in mass of 3-4 kDa.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Research Laboratories of Behringwerke AG, Marburg, Germany.
authors.forename: M C
authors.initials: MC
authors.lastname: Gehrmann
authors.forename: M
authors.initials: M
authors.lastname: Opper
authors.forename: H H
authors.initials: HH
authors.lastname: Sedlacek
authors.forename: K
authors.initials: K
authors.lastname: Bosslet
authors.forename: J
authors.initials: J
authors.lastname: Czech
mesh_terms: D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006224:Cricetinae; D018076:DNA, Complementary / Q000737:chemistry; D004220:Disulfides / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D004795:Enzyme Stability; D015870:Gene Expression; D005966:Glucuronidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009154:Mutation; D011994:Recombinant Proteins / Q000378:metabolism*; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D004220:Disulfides; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D005966:Glucuronidase; D010450:Endopeptidases