Integration and expression of human growth hormone gene in Caladium bicolor.
Human growth hormone (hGH) gene has been inserted into the plasmid pLGV1103 to give the recombinant plasmid pLB-9. It has been introduced into the agrobacterium containing plasmid pGV3850. The recombinant Ti plasmid pGL198(hGH) has been obtained by homologous recombination. The monocotyledon Caladium bicolor has been transferred with pGL198 (hGH) with the leaf-disk co-cultivation method, and transgenic plants have been regenerated. The results of nopaline analysis, NPT II detection Southern blot and Western blot show that the hGH gene was integrated into the genome of Caladium bicolor, and a 22-kD protein was synthesized in the transgenic plants.
Li
B J
BJ
Biotechnology Research Centre, Zhongshan University, Guangzhou, PRC.
Wang
J F
JF
Xu
Z F
ZF
Xu
Y Q
YQ
Yu
M Z
MZ
He
X
X
Shen
Y
Y
D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D014170:Transformation, Genetic
D011994:Recombinant Proteins; D013006:Growth Hormone