Applications of capillary electrophoresis in the eye-care pharmaceutical industry.
The speed and resolution of capillary electrophoresis (CE), combined with its low cost and low volume requirements, make it a promising method of separating eye-care pharmaceuticals and analyzing the ionic composition of tears. Capillary zone electrophoresis (CZE) offers linearity, precision, and recovery comparable to high-performance liquid chromatography (HPLC) at a fraction of its cost. In addition, CZE separates and quantitates cocoamphocarboxyglycinate in the presence of a non-ionic fatty acid amide surfactant seven times faster than HPLC. CE easily detects such cations as sodium and potassium in human tears.
Chadwick
R R
RR
Allergan Optical, Irvine, CA 92715-2002.
Hsieh
J C
JC
Resham
K S
KS
Nelson
R B
RB
D000111:Acetylcysteine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004345:Drug Industry; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D009883:Ophthalmic Solutions / Q000032:analysis*; D013666:Tears / Q000737:chemistry
D009883:Ophthalmic Solutions; D000111:Acetylcysteine