title: A new ATP-independent DNA endonuclease from Schizosaccharomyces pombe that recognizes cyclobutane pyrimidine dimers and 6-4 photoproducts.
abstract: We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA endonuclease (SPDE) catalyzes a single ATP-independent incision immediately 5' to the UV photoproduct and generates termini containing 3' hydroxyl and 5' phosphoryl groups. Based on these properties, we propose that SPDE may function in a DNA repair capacity, representing the initial recognition/cleavage step of a DNA excision repair pathway.
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Rollins Research Center, Atlanta, GA 30322.
authors.forename: K K
authors.initials: KK
authors.lastname: Bowman
authors.forename: K
authors.initials: K
authors.lastname: Sidik
authors.forename: C A
authors.initials: CA
authors.lastname: Smith
authors.forename: J S
authors.initials: JS
authors.lastname: Taylor
authors.forename: P W
authors.initials: PW
authors.lastname: Doetsch
authors.forename: G A
authors.initials: GA
authors.lastname: Freyer
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D008969:Molecular Sequence Data; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology*; D029702:Schizosaccharomyces pombe Proteins; D013379:Substrate Specificity; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers; D029702:Schizosaccharomyces pombe Proteins; D000255:Adenosine Triphosphate; D004247:DNA; D004706:Endodeoxyribonucleases; C105073:uve1 protein, S pombe