Cloning and expression of a cDNA encoding bovine muscarinic acetylcholine m3 receptor.
A cDNA clone encoding bovine muscarinic acetylcholine m3 receptor has been isolated from a bovine brain library. Sequencing of this clone has revealed a single open reading frame encoding a protein of 590 amino acids. Comparison with known muscarinic m3 receptor sequences from other species shows a high degree of conservation (92-98% homology) in the protein sequence. COS cells transfected with this cDNA expressed a single high-affinity (Kd = 43.9 pM) binding site for muscarinic receptor ligand N-[3H]methylscopolamine.
Lee
P H
PH
Division of Cell Biology, Burroughs Wellcome Co., Research Triangle Park, NC 27709.
Hodges
P K
PK
Glickman
F
F
Chang
K J
KJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011976:Receptors, Muscarinic / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D018076:DNA, Complementary; D011976:Receptors, Muscarinic