[Drug antagonism of TNF induced proliferation of bovine cerebromicrovascular smooth muscle cells].
Effects of tumor necrosis factor (TNF) on the proliferation of bovine cerebromicrovascular smooth muscle cells (BCSMC) were investigated. At concentrations from 50 to 5000 U.ml-1, TNF was shown to induce proliferation of cultured BCSMC in a dose-dependent manner. After 24 h incubation of the cells, TNF significantly stimulated the proliferation of BCSMC and reached maximal effects after 48 h incubation, then the effects slightly decreased. At concentrations from 10(-6) to 10(-4) mol.L-1, both imperatorin (Imp) and iso-imperatorin (Isi) were found to antagonize the TNF induced proliferation of BCSMC. Their maximal inhibitory effects were 42.2 and 36.1% at 10(-4) mol.L-1 respectively. 6-(alpha,alpha-diphenylacetylpiperazinly) phenyl-5-methyl-4,5-dihydro-3 (2H)-pyridazinone (DMDP) and 6-(alpha-phenylacetylpiperazinyl) phenyl-5-methyl-4,5-dihydro-3(2H)-pyridazinone (PMDP) were also found to possess similar effect at lower concentration (10(-6) mol.L-1), but no significant effect was observed when the drug concentration was higher.
Ji
Y
Y
Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai.
Zeng
G Q
GQ
Sun
D X
DX
D000818:Animals; D001921:Brain / Q000098:blood supply*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D011564:Furocoumarins / Q000494:pharmacology*; D008833:Microcirculation / Q000166:cytology; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D010879:Piperazines / Q000494:pharmacology*; D011724:Pyridazines / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
D011564:Furocoumarins; D010879:Piperazines; D011724:Pyridazines; D014409:Tumor Necrosis Factor-alpha; C055542:isoimperatorin; C088483:6-(phenylacetylpiperazinyl)phenyl-5-methyl-4,5-dihydro-3(2H)-pyridazinone; C083460:6-(diphenylacetylpiperazinyl)pheny-5-methyl-4,5-dihydro-3-(2H)-pyridazinone; C031534:imperatorin