Effects of infused amino acids and lipids on glucose metabolism in healthy lean humans.
The effects of infusion of a triglyceride emulsion (which induces peripheral insulin resistance) and amino acids (which stimulate gluconeogenesis) on glucose metabolism were investigated in healthy lean humans during exogenous infusion of glucose. One group of subjects (n = 5) was infused for 7.5 h with 11.1 mumol/kg/min glucose; during the last 4 h, amino acids were also infused at a rate of 3.33 mg/kg/min. A second group of subjects (n = 5) was infused with glucose+lipids (Lipovenös, 10% 10 ml/min) for 7.5 h and amino acids were added during the last 4 h. Infusion of lipids suppressed the increase in glucose oxidation observed during infusion of glucose alone (delta glucose oxidation: -2.1 +/- 1.1 vs. + 4.5 +/- 1.4 mumol/kg/min; P < 0.05) and during infusion of glucose+amino acids (delta glucose oxidation: + 1.6 +/- 1.4 vs. + 10.6 +/- 1.2 mumol/kg/min; P < 0.05). Gluconeogenesis (determined from 13C glucose synthesis during infusion of 13C bicarbonate) increased from 1.1 +/- 0.2 mumol/kg/min during infusion of glucose and 1.6 +/- 0.3 during infusion of glucose+lipids to 3.2 +/- 0.4 and 3.1 +/- 0.4, respectively, when amino acid infusion was superimposed (P < 0.05 in both instances). Plasma glucose concentrations were identical during infusion of glucose alone or glucose+amino acids, with or without lipids. Insulin concentrations were significantly increased by lipids both during infusion of glucose alone and of glucose+amino acids.(ABSTRACT TRUNCATED AT 250 WORDS)
Haesler
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E
Institute of Physiology, Lausanne University, School of Medicine, Switzerland.
Schneiter
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P
Temler
E
E
Jéquier
E
E
Tappy
L
L
D000328:Adult; D000596:Amino Acids / Q000008:administration & dosage / Q000378:metabolism*; D001786:Blood Glucose / Q000032:analysis; D001944:Breath Tests; D005217:Fat Emulsions, Intravenous; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D005934:Glucagon / Q000097:blood; D005943:Gluconeogenesis; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D007262:Infusions, Intravenous; D007328:Insulin / Q000097:blood; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D014280:Triglycerides / Q000008:administration & dosage / Q000378:metabolism*
D000596:Amino Acids; D001786:Blood Glucose; D005217:Fat Emulsions, Intravenous; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D014280:Triglycerides; D005934:Glucagon; D005947:Glucose; D009584:Nitrogen