Eicosapentaenoic acid enhances intracellular free calcium in cultured human endothelial cells.
Eicosapentaenoic acid (EPA) caused elevations of intracellular free Ca2+ concentration ([Ca2+]i) measured by fura-2 fluorescence in cultured human endothelial cells. The EPA-induced increase in [Ca2+]i could still be observed when either cyclooxygenase or lipoxygenase inhibitors were added. These results suggest that EPA itself rather than its metabolites has direct effects on intracellular Ca2+ mobilization, causing the elevation of [Ca2+]i.
Okuda
Y
Y
Department of Internal Medicine, University of Tsukuba, Ibaraki, Japan.
Ezure
M
M
Tsukahara
K
K
Sawada
T
T
Mizutani
M
M
Katori
T
T
Bannai
C
C
Yamashita
K
K
D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans
D015118:Eicosapentaenoic Acid; D002118:Calcium