title: Metabolism of benz[a]anthracene by human liver microsomes.
abstract: The metabolism of benz[a]anthracene (BA) by human hepatic microsomes was investigated. Only dihydrodiols were observed when BA was the substrate. No tetrahydrotetrols were detected, indicating lack of diol epoxide formation. The BA-dihydrodiols identified by GCMS analysis and comparison to authentic standards were BA-8,9-dihydrodiol (42.4% of total metabolites), BA-5,6-dihydrodiol (25%), BA-10,11-dihydrodiol (24.8%), BA-3,4-dihydrodiol (5.3%), and BA-1,2-dihydrodiol (< 1.5%). BA-dihydrodiols were also used individually as substrates. Only BA-1,2-dihydrodiol, the least abundant isomer produced from BA, was converted efficiently to a tetrahydrotetrol (> 72% conversion). BA-10,11-dihydrodiol was converted to BA-8,9,10,11-tetrahydrotetrols in < 12% yield. BA-10,11- and BA-3,4-dihydrodiols were not converted to tetrahydrotetrols.
authors.affiliation: Division of Toxicology, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: Y
authors.initials: Y
authors.lastname: Sahali
authors.forename: L R
authors.initials: LR
authors.lastname: Kidd
authors.forename: P L
authors.initials: PL
authors.lastname: Skipper
authors.forename: S R
authors.initials: SR
authors.lastname: Tannenbaum
mesh_terms: D001551:Benz(a)Anthracenes / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004852:Epoxy Compounds; D006801:Humans; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism*
chemical_list: D001551:Benz(a)Anthracenes; D004852:Epoxy Compounds; C030935:benz(a)anthracene