Mannosylated liposomes as carriers for hamycin in the treatment of experimental aspergillosis in Balb/C mice.
Hamycin incorporated into mannosylated liposomes produced less toxicity and enhanced antifungal activity in experimental aspergillosis in balb/c mice in vivo. Incorporation of cholesterol into mannosylated liposomes led to a decrease in hamycin toxicity. The LD50 (mg/kg) values of hamycin contained in SPC/Chol/DPPE-Man (molar ratio 4:5:1) lipsomes was 2.8 whereas that in SPC/DPPE-Man liposomes (molar ratio 9:1) was 1.4. Incorporation of cholesterol into mannosylated liposomes increased the survival rates of infected animals: 70% survival was recorded after 7 days therapy as well as reduced fungal load in lung, liver, spleen and kidney. HPLC studies of distribution of hamycin in various tissues showed a reduction in the concentration of the liposomal drug in circulation compared to that observed for free drug and neutral liposomes after 1 hour.
Moonis
M
M
Department of Biochemistry, University of Delhi, India.
Ahmad
I
I
Bachhawat
B K
BK
D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage* / Q000627:therapeutic use* / Q000633:toxicity; D001228:Aspergillosis / Q000188:drug therapy* / Q000382:microbiology; D002784:Cholesterol / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D015169:Colony Count, Microbial; D004337:Drug Carriers; D007928:Lethal Dose 50; D008081:Liposomes; D008297:Male; D008358:Mannose / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D011090:Polyenes / Q000008:administration & dosage / Q000627:therapeutic use / Q000633:toxicity; D014018:Tissue Distribution
D000935:Antifungal Agents; D004337:Drug Carriers; D008081:Liposomes; D011090:Polyenes; C100114:hamycin; D002784:Cholesterol; D008358:Mannose