Expression of tumor-associated protein variants in chemically induced rat hepatomas and transformed rat liver cell lines determined by two-dimensional electrophoresis.
High resolution two-dimensional electrophoresis (2-DE), using the rat liver as a model, was applied to study hepatocarcinogenesis induced by different chemical carcinogens. Several tumor-associated protein variants were detected by 2-DE in chemically induced rat hepatomas and transformed rat liver cell lines compared to normal rat liver tissue. Proliferation-related protein changes and/or protein alterations due to culture conditions were corrected for by comparison with 2-DE patterns of isolated cells from regenerating liver and short-term cultivated liver cells. Some of the tumor-associated variants were further characterized: (i) By peptide mapping with limited proteolysis we detected clear relationships between several variants. (ii) By studying post-translational modifications phosphorylated and glycosylated variants could be determined. (iii) A tumor-associated protein variant was identified by amino acid analysis and amino acid sequencing.
Zeindl-Eberhart
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Institute of Pathology, University of Munich, Germany.
Jungblut
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Rabes
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HM
D000449:Aldehyde Reductase / Q000096:biosynthesis / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D002461:Cell Line, Transformed; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D014644:Genetic Variation; D007202:Indicators and Reagents; D007525:Isoelectric Focusing / Q000379:methods; D007908:Lens, Crystalline / Q000201:enzymology; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D008770:Methylnitrosourea; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D010449:Peptide Mapping; D010761:Phosphorus Radioisotopes; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000302:isolation & purification; D051381:Rats; D017386:Sequence Homology, Amino Acid
D007202:Indicators and Reagents; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D010761:Phosphorus Radioisotopes; D011506:Proteins; D008770:Methylnitrosourea; D000449:Aldehyde Reductase