Glucan-binding proteins of Streptococcus sobrinus B13 grown in high glucose media.
To improve the yield of an 87-kDa glucan-binding protein (GBP) of Streptococcus sobrinus B13 (serogroup d), trypticase-yeast extract (TYE) medium supplemented with higher (1 and 2%) than the usual amount (0.2%) of glucose was used for growth. The production of this GBP extracellularly in 1.0 and 2.0% glucose-TYE media was examined and compared with the control (0.2% glucose). Upon analysis using SDS-PAGE, extracellular culture concentrates of 1.0 and 2.0% glucose-TYE cultures revealed similar protein profiles as the control. Higher glucose concentrations did not inhibit the synthesis of the 87-kDa GBP. Cells grown in 1.0 or 2.0% glucose-supplemented media aggregated rapidly compared to those observed in the control cells (0.2% glucose grown). Higher cell yield and higher extracellular protein content were obtainable in both 1.0 and 2% glucose-TYE cultures, thus improving the yield of the 87 kDa GBP.
Wu-Yuan
C D
CD
Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City 52242-1010.
Scheinost
M
M
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002352:Carrier Proteins / Q000096:biosynthesis*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005936:Glucans / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D013291:Streptococcus / Q000254:growth & development / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003470:Culture Media; D005936:Glucans; D005947:Glucose