Characterization of pilin genes from seven serologically defined prototype strains of Moraxella bovis.
Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characterized by nucleotide sequence determination. The coding sequences show extensive homology (70 to 80%) while the proximal downstream sequences show a dichotomy into nonhomologous sets. The pilin genes of three more strains were also characterized. The presence of an additional, partial pilin gene in each prototype strain was confirmed by Southern blot analysis, and the partial pilin genes from two strains of one serogroup were characterized by sequence determination. Features of the pilin gene sequences are considered in relation to pilin gene inversion and the serological variants of strains which may arise from gene inversion events.
Atwell
J L
JL
Division of Biomolecular Engineering, Commonwealth Scientific and Industrial Research Organisation, Parkville, Victoria, Australia.
Tennent
J M
JM
Lepper
A W
AW
Elleman
T C
TC
D001315:Australia; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D003062:Codon; D004269:DNA, Bacterial / Q000235:genetics; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016955:Moraxella bovis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping
D001425:Bacterial Outer Membrane Proteins; D003062:Codon; D004269:DNA, Bacterial; D036781:Fimbriae Proteins