Reduction in muscarinic receptors by antisense oligodeoxynucleotide.
Treatment of rat superior cervical ganglion cells in culture for 4 days with an antisense oligodeoxynucleotide corresponding to a partial sequence of the mRNA coding the m1 muscarinic receptor decreased m1 receptor mRNA by 64% without affecting m2 receptor mRNA. [3H]Pirenzepine binding to M1 receptors was decreased by 43%. Intraventricular infusion of this antisense oligodeoxynucleotide reduced muscarinic receptor density in M1 but not M2 receptor-rich brain regions.
Zang
Z
Z
Center for Molecular and Behavioral Neuroscience, Rutgers, State University of New Jersey, Newark 07102.
Florijn
W
W
Creese
I
I
D000818:Animals; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011976:Receptors, Muscarinic / Q000187:drug effects* / Q000235:genetics; D016914:Ribonuclease H / Q000378:metabolism; D017783:Superior Cervical Ganglion / Q000187:drug effects / Q000378:metabolism
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D016914:Ribonuclease H