title: In vitro adhesion and invasion of Salmonella enterica serovar Havana.
abstract: A total of 132 isolates of the Havana serovar of Salmonela enterica were studied for their adhesion to and invasion of Hela cells in the presence of D-mannose. A total of 124 (93.9%) isolates were adherent, and 51% of these were invasive. Of these 124 strains 82 (66%) adhered to HeLa cells with a pattern typical for enteroaggregative (EAgg) Escherichia coli whereas the remaining 34% adhered in a non-aggregative (non-EAgg) pattern. Of the 82 strains showing the EAgg pattern 60% were invasive whereas only 33% of the 49 non-EAgg isolates invaded HeLa cells (P < 0.01). The action of mannose-sensitive type 1 pili was inhibited by the addition of D-mannose. Therefore we conclude that type 1 pili are not important in adhesion and invasion of Havana.
authors.affiliation: Molecular Biology Unit, Pasteur Institute of Iran, Tehran.
authors.forename: A
authors.initials: A
authors.lastname: Jafari
authors.forename: S
authors.initials: S
authors.lastname: Bouzari
authors.forename: A A
authors.initials: AA
authors.lastname: Farhoudi-Moghaddam
authors.forename: M
authors.initials: M
authors.lastname: Parsi
authors.forename: F
authors.initials: F
authors.lastname: Shokouhi
mesh_terms: D001422:Bacterial Adhesion; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D006367:HeLa Cells; D006801:Humans; D007492:Iran / Q000453:epidemiology; D012475:Salmonella / Q000472:pathogenicity*; D012703:Serotyping; D014774:Virulence