Inhibition of aflatoxin B1-DNA binding and adduct formation by selenium in rats.
The effect of selenium on aflatoxin B1-DNA binding and adduct formation was studied. Male Fischer 344 rats, fed with up to 8 ppm of sodium selenite in drinking water for 8 weeks, were given a single i.p. dose of aflatoxin B1. The rats were killed 24 h later and the amount of AFB1 bound to hepatic DNA and the amount of DNA adducts formed were determined. Selenium pretreatment resulted in a dose-dependent inhibition of AFB1-DNA binding as well as adduct formation. This was accompanied by an increase of reduced glutathione (GSH) in the liver of selenium-treated animals. These results suggest that selenium could effectively inhibit AFB1-induced DNA damage, which may be partially responsible for its anticarcinogenic effect against AFB1.
Shi
C Y
CY
Department of Community, Occupational and Family Medicine, National University of Singapore.
Chua
S C
SC
Lee
H P
HP
Ong
C N
CN
D016604:Aflatoxin B1 / Q000378:metabolism*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism; D019803:Glutathione Disulfide; D008099:Liver / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D012643:Selenium / Q000097:blood / Q000494:pharmacology*
D018736:DNA Adducts; C072611:aflatoxin B1-DNA adduct; D004247:DNA; D016604:Aflatoxin B1; D005978:Glutathione; D012643:Selenium; D019803:Glutathione Disulfide