Thyroid hormone receptor messenger ribonucleic acid in human granulosa and ovarian stromal cells.
OBJECTIVE
To examine whether mRNA for thyroid hormone receptors alpha and beta is present in human granulosa cells in nonstimulated ovaries.


METHODS
Paraffin-embedded sections of ovaries from normally cycling women were analyzed by in situ hybridization with oligonucleotide probes for thyroid hormone receptors alpha and beta. The sense strand oligonucleotide was used as a control for each of the probes.


RESULTS
Granulosa cells from the preovulatory antral follicles examined showed positive staining for both the thyroid hormone receptor alpha and beta probes. Positive staining of ovarian stromal cells also was observed for both probes.


CONCLUSIONS
Thyroid hormone receptor mRNAs are expressed in both granulosa cells and ovarian stromal cells found in nonstimulated ovaries. It is, therefore, conceivable that thyroid hormone may play a direct role in human ovarian physiology.
Wakim
A N
AN
Medical College of Pennsylvania Allegheny Campus, Pittsburgh.
Paljug
W R
WR
Jasnosz
K M
KM
Alhakim
N
N
Brown
A B
AB
Burholt
D R
DR
D000328:Adult; D005260:Female; D005498:Follicular Phase; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011988:Receptors, Thyroid Hormone / Q000235:genetics*; D017154:Stromal Cells / Q000378:metabolism*
D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone