Rapid detection and differentiation of tobamoviruses infecting L-resistant genotypes of pepper by RT-PCR and restriction analysis.
A procedure involving reverse transcription followed by polymerase chain reaction (RT-PCR) was developed for typing pathotypes of the tobamoviruses infecting the L-resistant genotypes of pepper. The method provides a much simpler alternative to the bioassay tests for the different Capsicum spp. genotypes previously used. Discrimination between the two pathotypes, P1,2 and P1,2,3, which cannot be differentiated by serological means, was achieved by restriction enzyme analysis of the PCR products. The assay also detects and distinguishes both pathotypes in a single mixed-infected plant. The procedure should be useful for the diagnosis and control of the disease and helpful to breeders and biotechnologists when producing and evaluating resistance in pepper plants.
Tenllado
F
F
Departamento de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
García-Luque
I
I
Serra
M T
MT
Díaz-Ruiz
J R
JR
D001483:Base Sequence; D002212:Capsicum / Q000235:genetics / Q000382:microbiology*; D004352:Drug Resistance, Microbial; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D013045:Species Specificity; D013997:Time Factors; D017898:Tobamovirus / Q000302:isolation & purification*
D012367:RNA, Viral