Unique localization of fucosyl GM1 in rabbit spinal cord and peripheral nerve: immunohistochemical study using monoclonal anti-fucosyl GM1 antibody CRD73-6.
We generated a murine monoclonal antibody (CRD73-6) for ganglioside fucosyl GM1 using a homogenate of rabbit dorsal root ganglia as an immunogen. CRD73-6 immunostained a subset of neurons and the satellite cells surrounding them in rabbit dorsal root ganglia, unmyelinated fibers in the dorsal root, and dorsal horn of the spinal cord. After the tissue sections had been treated with acetone, CRD73-6 also immunostained large neurons and the perineuronal area around them in the ventral horn, and axons and myelin of the small myelinated fibers in the dorsal root. Unique localization of fucosyl GM1 implicates its possible role in cell adhesion and recognition in the nervous system.
Kusunoki
S
S
Department of Neurology, Faculty of Medicine, University of Tokyo, Japan.
Chiba
A
A
Shimizu
T
T
Kanazawa
I
I
D000096:Acetone / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000737:chemistry; D002240:Carbohydrate Sequence; D005260:Female; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D005727:Ganglia, Spinal / Q000737:chemistry; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009186:Myelin Sheath / Q000737:chemistry; D009474:Neurons / Q000737:chemistry; D010525:Peripheral Nerves / Q000737:chemistry*; D011817:Rabbits; D013116:Spinal Cord / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D000096:Acetone; D005677:G(M1) Ganglioside; C039123:fucosyl GM1 ganglioside