title: A dipeptidylpeptidase secreted by Fasciola hepatica.
abstract: A dipeptidylpeptidase (DPP) was isolated from Fasciola hepatica by gel-filtration and ion-exchange chromatography. The exoproteinase is secreted by newly excysted juveniles, immature and mature flukes. The liver fluke DPP is a serine proteinase of molecular weight > 200 kDa and differs from previously characterized mammalian DPPs in its substrate preference and susceptibility to inactivation by inhibitors. The parasite DPP may function in the latter stages of the proteolytic digestion of host macromolecules. In this manner, the enzyme may be important in providing the parasite with dipeptides that could be absorbed through the intestine as nutrient.
authors.affiliation: School of Biological Sciences, Dublin City University, Republic of Ireland.
authors.forename: C
authors.initials: C
authors.lastname: Carmona
authors.forename: S
authors.initials: S
authors.lastname: McGonigle
authors.forename: A J
authors.initials: AJ
authors.lastname: Dowd
authors.forename: A M
authors.initials: AM
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Coughlan
authors.forename: E
authors.initials: E
authors.lastname: McGowran
authors.forename: J P
authors.initials: JP
authors.lastname: Dalton
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D005210:Fasciola hepatica / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000469:parasitology; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D013379:Substrate Specificity
chemical_list: D011480:Protease Inhibitors; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases