A patch-clamp study on a novel gamma-aminobutyric acid activated chloride channel of crayfish deep extensor abdominal muscle.
The patch-clamp technique was used to study the effect of rapid pulses of gamma-aminobutyric acid (GABA) on excised membrane-patches in the outside-out configuration of the deep extensor abdominal muscle (DEAM) of crayfish. Channel currents reversed at the equilibrium potential of Cl- and were blocked by picrotoxin. Rare channel openings were elicited by 0.1 mM GABA, and a saturating open probability of 0.9 was reached with 10 nM GABA. The investigated channel was only sensitive to GABA and is different from a previously described GABAergic channel in crayfish that is in addition sensitive to acetylcholine and glutamate and shows three subconductance states.
Adelsberger
H
H
Physiologisches Institute der Technischen Universität München, FRG.
von Beckerath
N
N
Franke
C
C
Dudel
J
J
D000009:Abdominal Muscles / Q000378:metabolism*; D000818:Animals; D003400:Astacoidea / Q000378:metabolism*; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000502:physiology*; D004594:Electrophysiology; D010852:Picrotoxin / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D018118:Chloride Channels; D010852:Picrotoxin; D005680:gamma-Aminobutyric Acid