The effect of cell confluency state on the expression of neural cell surface glycoconjugates.
The expression of neural cell surface glycoconjugates during development reflects the precise control of cellular adhesivity that is required for the exact positioning of the developing cells. We have investigated the effect of cell confluency state on the expression of key cell surface glycoconjugates using the mouse neuroblastoma cell line, neuro-2A. There was an up-regulation of expression of the neural cell adhesion molecule (NCAM) coincident with an increase in cell confluency state and a parallel decrease in the expression of the amyloid precursor protein, beta APP. The expression pattern of cell surface glycoconjugates in neuro-2A cells is therefore similar to that observed during the early embryonic period of development during which cells aggregate to form collectives.
Breen
K C
KC
Dept. of Pharmacology and Clinical Pharmacology, University of Dundee, Ninewells Hospital and Medical School, Scotland, UK.
Ronayne
E
E
D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis / Q000276:immunology; D000818:Animals; D001932:Brain Neoplasms / Q000503:physiopathology; D002448:Cell Adhesion / Q000502:physiology; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis*; D002455:Cell Division / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D009447:Neuroblastoma / Q000503:physiopathology; D009474:Neurons / Q000378:metabolism* / Q000502:physiology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000502:physiology
D016564:Amyloid beta-Protein Precursor; D015816:Cell Adhesion Molecules, Neuronal; D008562:Membrane Glycoproteins