title: Alternative patterns of his operon transcription and mRNA processing generated by metabolic perturbation.
abstract: Previous studies have shown that the expression of the his operon of Salmonella typhimurium is regulated at the level of transcription initiation, transcription elongation and RNA processing. We have analyzed his RNA in both prototrophic strains or strains harboring regulatory and auxotrophic mutations grown under a variety of metabolic conditions that lead to differential expression of the operon. Under some of these conditions, there is an increase in the amount of prematurely released his-specific RNA, resulting in modulation of the relative amount of full-length transcripts. Under the same metabolic conditions, there is also a modulation of RNA processing events that generate a very stable RNA species comprising the five distal cistrons. These effects appear to be due to perturbation of the translation process caused by alterations in the intracellular pool of initiator transfer RNA.
authors.affiliation: Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università degli Studi di Napoli, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Alifano
authors.forename: F
authors.initials: F
authors.lastname: Rivellini
authors.forename: A G
authors.initials: AG
authors.lastname: Nappo
authors.forename: C B
authors.initials: CB
authors.lastname: Bruni
authors.forename: M S
authors.initials: MS
authors.lastname: Carlomagno
mesh_terms: D015964:Gene Expression Regulation, Bacterial; D006639:Histidine / Q000235:genetics*; D009876:Operon; D012333:RNA, Messenger; D012485:Salmonella typhi / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D006639:Histidine