Identification of a target protein of US3 protein kinase of herpes simplex virus type 2.
Herpes simplex virus type 2 (HSV-2) gene US3 has been shown to encode a serine-threonine protein kinase. In this study, we have tried to identify target proteins of the US3 protein kinase using a US3 lacZ insertion mutant of HSV-2. When permeabilized cells were labelled with [gamma-32P]ATP under the optimum conditions for the US3 enzyme, the most striking difference between wild-type HSV-2 strain 186- and mutant-infected cells was observed in the phosphorylation of proteins ranging in M(r) values from 14K to 22K. Studies of in vitro phosphorylation with purified virions and with cells infected with a US9-defective HSV-1 mutant suggested that a tegment phosphoprotein encoded by the US9 gene may be a target of HSV-2 US3 protein kinase.
Daikoku
T
T
Laboratory of Virology, Nagoya University School of Medicine, Japan.
Kurachi
R
R
Tsurumi
T
T
Nishiyama
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D018258:Herpesvirus 2, Human / Q000201:enzymology / Q000378:metabolism*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism*
D009842:Oligopeptides; D014764:Viral Proteins; D011794:Quercetin; D017346:Protein Serine-Threonine Kinases; C084395:US3 protein, Human herpesvirus 2