Localization of Rabphilin-3A on the synaptic vesicle.
Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein which is implicated in neurotransmitter release. Rabphilin-3A is expressed mainly in brain, but its subcellular localization remains to be clarified. Immunohistochemical analysis has revealed that Rabphilin-3A is most abundant in the synaptic area of the rat cerebellum, retina, and neuromuscular junction. Ultrastructural analysis of the neuromuscular junction using the immunogold method indicates that Rabphilin-3A is localized on the synaptic vesicle. Subcellular fractionation analysis of rat brain has shown that Rabphilin-3A is most highly concentrated in the purified synaptic vesicle fraction. These results indicate that Rabphilin-3A is localized on the synaptic vesicle in the presynapse.
Mizoguchi
A
A
Department of Anatomy, Kobe University School of Medicine, Japan.
Yano
Y
Y
Hamaguchi
H
H
Yanagida
H
H
Ide
C
C
Zahraoui
A
A
Shirataki
H
H
Sasaki
T
T
Takai
Y
Y
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002458:Cell Fractionation; D002531:Cerebellum / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism; D013572:Synaptic Vesicles / Q000378:metabolism*; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D000097624:Rabphilin-3A
D048868:Adaptor Proteins, Signal Transducing; D019204:GTP-Binding Proteins; D009419:Nerve Tissue Proteins; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D000097624:Rabphilin-3A