A high-yield method for site-directed mutagenesis using polymerase chain reaction and three primers.
Steinberg
R A
RA
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Gorman
K B
KB
D001482:Base Composition; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase; D007202:Indicators and Reagents; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping; D013698:Templates, Genetic
D017931:DNA Primers; D007202:Indicators and Reagents; D004259:DNA-Directed DNA Polymerase