Localization of a putative tumor suppressor gene by using homozygous deletions in melanomas.
The p21 region of human chromosome 9 is thought to contain a gene (MLM) involved in genetic susceptibility to melanoma and a gene or genes that influence progression of certain other tumors. Genomic clones that span a large region in 9p21 surrounding the presumptive tumor suppressor gene(s) have been isolated. A set of sequence-tagged sites in this region has been developed. By using these markers and others previously reported, the 9p21 region has been studied by physical mapping in 84 melanoma cell lines. A putative tumor suppressor gene, perhaps MLM itself, has been localized to a region of less than 40 kb that lies proximal (centromeric) to the alpha-interferon gene cluster.
Weaver-Feldhaus
J
J
Myriad Genetics, Inc., Salt Lake City, UT 84108.
Gruis
N A
NA
Neuhausen
S
S
Le Paslier
D
D
Stockert
E
E
Skolnick
M H
MH
Kamb
A
A
D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D006720:Homozygote; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D017384:Sequence Deletion; D016324:Sequence Tagged Sites; D014407:Tumor Cells, Cultured
D005819:Genetic Markers