Effects of butyrate on the erythropoietin receptor of cell line IW201.
The murine erythroleukemic cell line, IW201, normally expresses only low-affinity erythropoietin receptors. Exposure of these cells for 48 hours to sodium butyrate results in a change in receptor kd from about 600 pmol/L to 100 to 200 pmol/L. This change in affinity is accompanied by downregulation of both receptor number and receptor mRNA. Cells exposed to sodium butyrate for 2 hours show a similar change in kd but no change in receptor number. The butyrate effect on kd at 2 hours is abrogated by either cycloheximide or actinomycin D. These data indicate that an accessory protein induced by sodium butyrate is responsible for high-affinity binding of erythropoietin.
Hermine
O
O
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
Dong
Y J
YJ
Goldwasser
E
E
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D015536:Down-Regulation; D004921:Erythropoietin / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D066298:In Vitro Techniques; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D017467:Receptors, Erythropoietin / Q000378:metabolism*
D002087:Butyrates; D012333:RNA, Messenger; D017467:Receptors, Erythropoietin; D004921:Erythropoietin; D003609:Dactinomycin; D003513:Cycloheximide