title: Purification and characterization of recombinant human sickle hemoglobin expressed in yeast.
authors.affiliation: Department of Biochemistry, Rockefeller University, New York, New York 10021.
authors.forename: J J
authors.initials: JJ
authors.lastname: Martin de Llano
authors.forename: O
authors.initials: O
authors.lastname: Schneewind
authors.forename: G L
authors.initials: GL
authors.lastname: Stetler
authors.forename: J M
authors.initials: JM
authors.lastname: Manning
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D002266:Carboxymethylcellulose Sodium; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005914:Globins / Q000235:genetics; D006441:Hemoglobin A / Q000235:genetics; D006451:Hemoglobin, Sickle / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development
chemical_list: D000596:Amino Acids; D018076:DNA, Complementary; D006451:Hemoglobin, Sickle; D011994:Recombinant Proteins; D005914:Globins; D006441:Hemoglobin A; D002266:Carboxymethylcellulose Sodium