Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression.
A full-length cDNA clone encoding a potato invertase (Inv) has been isolated. It is highly related (77% nucleotide identity) to a previously characterised potato cDNA clone encoding a putative extracellular Inv. These Inv genes encode a subfamily of apoplastic enzymes which are shown to be distinct, on the basis of sequence similarity, from the related subfamily of vacuolar enzymes. In order to differentiate between the expression of the two potato genes encoding apoplastic Inv, a single-stranded conformational polymorphism (SSCP) assay was developed for products generated by reverse transcription-polymerase chain reaction (RT-PCR) utilising primers designed to amplify both potato sequences. Using this approach, we have shown that these two identified Inv from potato are expressed in a tissue-specific and developmentally regulated manner.
Hedley
P E
PE
Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Machray
G C
GC
Davies
H V
HV
Burch
L
L
Waugh
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D018087:Plastids; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics*; D014018:Tissue Distribution; D043324:beta-Fructofuranosidase
D018076:DNA, Complementary; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase