A fatty-acid-binding protein from wheat kernels.
A protein of about 7 kDa (W-FABP) has been isolated from mature wheat kernels by H2O extraction and gel filtration of the extract, followed by two steps of high-performance liquid chromatography. The N-terminal amino acid-sequence has been determined up to the 28th residue and found to be identical (except for positions 4 and 5) to that deduced from a barley cDNA (EMBL X15257), which had been improperly classified as a non-specific lipid transfer protein (LTP2). Similarly with LTPs, W-FABP does bind fatty acids, but in contrast, it is not significantly homologous to LTPs, it is not recognized by LTP antibodies, it has a more acidic isoelectric point (pH < 6.8 vs. pH > 9.6), and it does not show antibiotic properties.
Castagnaro
A
A
Laboratorio de Bioquímica y Biología Molecular, ETS Ingenieros Agrónomos, UPM, Madrid, Spain.
García-Olmedo
F
F
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D001467:Hordeum / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D017421:Sequence Analysis; D013045:Species Specificity; D014908:Triticum / Q000378:metabolism*
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins; D009829:Oleic Acids; D019301:Oleic Acid