A temporal study of RNAs produced in maedi-visna virus infection of choroid plexus cells.
We have examined the types and cellular distribution of transcripts of maedi-visna virus during infection of choroid plexus fibroblasts. Early in infection (19 hours post infection) only small spliced transcripts are found in the cytoplasm. Little virus specific RNA is detected in the nucleus at this stage. Later in infection structural gene transcripts are detectable in the cytoplasm as well as the nucleus. We have measured the half life of each type of transcript in total cellular RNA late in infection by two different methods (northern blotting or S1 protection assays after alpha-amanitin treatment, or specific hybridisation after pulse chase). Both of these methods suggest half lives for unspliced viral RNAs of 8-12 hours. Although the gel based methods were unsuitable for the accurate determination of half life for the shorter transcripts, they did confirm a long half life for these transcripts: pulse chase measurements suggested that this was similar to that seen for the unspliced transcripts. These observations are consistent with the virus exerting temporal control on protein synthesis by a method analogous to that of the HIV Rev protein.
Sargan
D R
DR
Department of Veterinary Pathology, University of Edinburgh, Summerhall, UK.
Roy
D J
DJ
Dalziel
R G
RG
Watt
N J
NJ
McConnell
I
I
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002831:Choroid Plexus / Q000166:cytology / Q000382:microbiology*; D005347:Fibroblasts / Q000382:microbiology; D015694:Gene Products, rev / Q000502:physiology; D015696:Gene Products, tat / Q000502:physiology; D006207:Half-Life; D015347:RNA Probes; D012326:RNA Splicing; D012367:RNA, Viral / Q000096:biosynthesis*; D015183:Restriction Mapping; D012756:Sheep; D013997:Time Factors; D014790:Visna-maedi virus / Q000235:genetics*
D015694:Gene Products, rev; D015696:Gene Products, tat; D015347:RNA Probes; D012367:RNA, Viral