title: Autoradiographic quantification of muscarinic cholinergic synaptic markers in bat, shrew, and rat brain.
abstract: We employed radioligand binding autoradiography to determine the distributions of pre- and post-synaptic cholinergic radioligand binding sites in the brains of two species of bat, one species of shrew, and the rat. High affinity choline uptake sites were measured with [3H]hemicholinium, and presynaptic cholinergic vesicles were identified with [3H]vesamicol. Muscarinic cholinergic receptors were determined with [3H]scopolamine. The distribution patterns of the three cholinergic markers were similar in all species examined, and identified known major cholinergic pathways on the basis of enrichments in both pre- and postsynaptic markers. In addition, there was excellent agreement, both within and across species, in the regional distributions of the two presynaptic cholinergic markers. Our results indicate that pharmacological identifiers of cholinergic pathways and synapses, including the cholinergic vesicle transport site, and the organizations of central nervous system cholinergic pathways are phylogenetically conserved among eutherian mammals.
authors.affiliation: Dept. of Neurology, University of Michigan, Ann Arbor.
authors.forename: R L
authors.initials: RL
authors.lastname: Albin
authors.forename: M M
authors.initials: MM
authors.lastname: Howland
authors.forename: D S
authors.initials: DS
authors.lastname: Higgins
authors.forename: K A
authors.initials: KA
authors.lastname: Frey
mesh_terms: D000818:Animals; D001345:Autoradiography; D015415:Biomarkers / Q000737:chemistry; D001923:Brain Chemistry / Q000502:physiology*; D002685:Chiroptera / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000032:analysis*; D012788:Shrews / Q000378:metabolism*; D013569:Synapses / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D011976:Receptors, Muscarinic