Daunomycin as an inhibitor of human lens epithelial cell proliferation in culture.
Posterior capsule opacification is still a major complication of both extracapsular cataract extraction and phacoemulsification. We evaluated the effects of the anti-proliferative agent daunomycin on cultured human and bovine lens epithelial cell viability and proliferation. After ten minutes of exposure, low concentrations of the agent markedly inhibited the proliferation of both cell types. The calculated LD50 for the drug against human cells was 2.20 micrograms/ml and against the bovine cells was 0.38 microgram/ml. The bovine cells appeared to be slightly more susceptible to the drug's effects, although this difference was not marked. Our results indicate that daunomycin is a potent inhibitor of both human and bovine lens epithelial cells in the laboratory.
Power
W J
WJ
Department of Ophthalmology, Royal Victoria Eye and Ear Hospital, Dublin, Ireland.
Neylan
D
D
Collum
L M
LM
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003630:Daunorubicin / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects*; D007928:Lethal Dose 50
D003630:Daunorubicin