Cataloging of the genes expressed in human keratinocytes: analysis of 607 randomly isolated cDNA sequences.
The partial nucleotide sequences of 607 cDNAs randomly isolated from a cDNA library of cultured human epidermal keratinocytes were determined by single pass sequencing. Homology search of the sequences to the non-redundant nucleotide databases revealed that 27% of the cDNAs matched registered human-or non-human genes encoding not only keratinocyte specific genes, but also a variety of functional proteins, the expression of which had not been identified in keratinocytes. Non-matching cDNAs covering 49% of the cDNAs were not homologous even to ESTs from other organs, suggesting that these cDNAs include novel genes expressed in the cells. The large scale sequencing of keratinocyte cDNAs provides a useful molecular source for research into biology and diseases of the skin.
Konishi
K
K
Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
Morishima
Y
Y
Ueda
E
E
Kibe
Y
Y
Nonomura
K
K
Yamanishi
K
K
Yasuno
H
H
D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000032:analysis*; D016208:Databases, Factual; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
D018076:DNA, Complementary