Cloning and expression analysis of the cDNA encoding rat Zn-alpha 2-glycoprotein.
We report here the nucleotide (nt) sequence of a rat liver cDNA encoding Zn-alpha 2-glycoprotein (Zn-alpha 2-gp), a plasma protein with a high degree of sequence similarity to class-I major histocompatibility complex (MHC) antigens. The deduced amino acid (aa) sequence contains the coding information for 293 aa residues and shows 60% identity with the aa sequence of human Zn-alpha 2-gp and 35% identity with that corresponding to the extracellular domains of RT1, a rat class-I MHC antigen. Northern blot analysis showed that rat Zn-alpha 2-gp is expressed in liver, but not in a wide number of tissues, including prostate, mammary gland, kidney, intestine, lung, pancreas, ovary, uterus, thyroid, placenta, spleen, brain and heart.
Fueyo
A
A
Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Uría
J A
JA
Freije
J M
JM
López-Otín
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D029589:Seminal Plasma Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D000090203:Zn-Alpha-2-Glycoprotein
D018076:DNA, Complementary; D006023:Glycoproteins; D015395:Histocompatibility Antigens Class I; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein