title: Inhibition of aflatoxin B1-DNA binding and adduct formation by selenium in rats.
abstract: The effect of selenium on aflatoxin B1-DNA binding and adduct formation was studied. Male Fischer 344 rats, fed with up to 8 ppm of sodium selenite in drinking water for 8 weeks, were given a single i.p. dose of aflatoxin B1. The rats were killed 24 h later and the amount of AFB1 bound to hepatic DNA and the amount of DNA adducts formed were determined. Selenium pretreatment resulted in a dose-dependent inhibition of AFB1-DNA binding as well as adduct formation. This was accompanied by an increase of reduced glutathione (GSH) in the liver of selenium-treated animals. These results suggest that selenium could effectively inhibit AFB1-induced DNA damage, which may be partially responsible for its anticarcinogenic effect against AFB1.
authors.affiliation: Department of Community, Occupational and Family Medicine, National University of Singapore.
authors.forename: C Y
authors.initials: CY
authors.lastname: Shi
authors.forename: S C
authors.initials: SC
authors.lastname: Chua
authors.forename: H P
authors.initials: HP
authors.lastname: Lee
authors.forename: C N
authors.initials: CN
authors.lastname: Ong
mesh_terms: D016604:Aflatoxin B1 / Q000378:metabolism*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism; D019803:Glutathione Disulfide; D008099:Liver / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D012643:Selenium / Q000097:blood / Q000494:pharmacology*
chemical_list: D018736:DNA Adducts; C072611:aflatoxin B1-DNA adduct; D004247:DNA; D016604:Aflatoxin B1; D005978:Glutathione; D012643:Selenium; D019803:Glutathione Disulfide