[A morphological evaluation of the protective properties of noninvasive recombinant strains of Shigella].
In 2-3 weeks after the oral immunization of rabbits, made in one or two administrations, with attenuated two-marker S. dysenteriae 1 strain VS-12 and recombinant S. dysenteriae VS-12/S. sonnei NR-18 and S. flexneri y433/S. sonnei NR-18 pronounced immunological reaction developed in the mucous membrane of the small intestine: blast transformation follicles of Peyer's patches, an increase in the number of lymphoblasts and plasmocytes in the cupolae of follicles and in intestinal villi, and an increase in the number of lymphocytes and macrophages in the intestinal epithelium with their release into the lumen of the intestine after challenge with virulent shigellae. The protective potency of these recombinants after challenge with massive doses of virulent shigellae was found to be high, which was shown by quantitative evaluation of the decrease of adhesion, invasiveness and cytotoxicity, suppression of epithelial lesions and development of inflammation in the intestinal mucosa.
Polotskiĭ
Iu E
IuE
Efremov
V E
VE
Bondarenko
V M
VM
Nastichkin
I A
IA
Belov
A G
AG
Kan
I M
IM
Smirnova
G V
GV
Romanova
Iu M
IuM
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D004353:Drug Evaluation, Preclinical; D004405:Dysentery, Bacillary / Q000276:immunology / Q000473:pathology* / Q000517:prevention & control*; D007114:Immunization; D007125:Immunogenetics; D007421:Intestine, Small / Q000276:immunology / Q000473:pathology; D011817:Rabbits; D011995:Recombination, Genetic; D012762:Shigella dysenteriae / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D012763:Shigella flexneri / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D012764:Shigella sonnei / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000276:immunology; D014774:Virulence
D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated
Morfologicheskaia otsenka protektivnosti neinvazivnykh rekombinantnykh shtammov shigell.