Organization and chromosomal location of the major histone cluster in brown trout, Atlantic salmon and rainbow trout.
The major histone cluster (hisDNA) was mapped by fluorescent in situ hybridization (FISH) to mitotic chromosomes of Atlantic salmon, brown trout, and rainbow trout. The data reveal that in the three species hisDNA is tandemly repeated in a single locus. Southern blots of genomic DNA indicate that these clusters are representative of the vast majority of the histone genes in these species. Similar reiteration values were found among the three species. Genetic variability in the hisDNA was found only in brown trout for an EcoRI site.
Pendás
A M
AM
Departamento de Biologia Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Morán
P
P
García-Vázquez
E
E
D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes; D014644:Genetic Variation; D006657:Histones / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D005810:Multigene Family / Q000235:genetics*; D017686:Oncorhynchus mykiss / Q000235:genetics; D012474:Salmon / Q000235:genetics; D012487:Salmonidae / Q000235:genetics*; D014337:Trout / Q000235:genetics
D006657:Histones