Molecular cloning and characterization of the genes encoding the immunoreactive major cell-surface proteins of Porphyromonas gingivalis.
A 72-kDa major cell-surface protein (72K-CSP) was purified from the wash fluid of Porphyromonas gingivalis OMZ409. Using the synthetic oligonucleotide probes corresponding to the determined amino-terminal amino acid sequence of 72K-CSP, recombinant plasmid clones carrying approx. 3.4-kb KpnI-XhoI fragments in XL1-Blue libraries of P. gingivalis OMZ409 and 381 were obtained. The premature form proteins of 558 and 563 amino acids led by putative signal sequences were thought to be processed to form the mature proteins of a predicted size of 55,655 Da for strain OMZ409 and of 55,654 for strain 381. Both proteins had unusual proline-rich regions in their carboxyl-terminal regions. No homologous sequences could be found in protein databases. Examination of antigen-specific antibody responses in the serum of patients with adult periodontitis by ELISA revealed that 72K-CSP had a different immunoreactivity from that of P. gingivalis 381 fimbriae.
Ogawa
T
T
Department of Oral Microbiology, Osaka University Faculty of Dentistry, Suita-Osaka, Japan.
Mori
H
H
Yasuda
K
K
Hasegawa
M
M
D000328:Adult; D000595:Amino Acid Sequence; D000906:Antibodies / Q000097:blood; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D010518:Periodontitis / Q000097:blood / Q000276:immunology; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000235:genetics*
D000906:Antibodies; D007074:Immunoglobulin G; D008565:Membrane Proteins