Molecular cloning and sequencing of equine interleukin 4.
We have cloned equine interleukin 4 (IL-4) cDNA using the polymerase chain reaction (PCR) and primers based on the human IL-4 sequence. The cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC). The cloned PCR product shares extensive homology ith IL-4 sequences from other species.
Vandergrifft
E V
EV
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70808.
Swiderski
C E
CE
Horohov
D W
DW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D006736:Horses / Q000276:immunology*; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D015847:Interleukin-4; D004247:DNA