[Diagnosis of experimental diabetes mellitus using isoelectric focusing of hemoglobin].
Glycosylated hemoglobin levels were measured in dogs with experimental diabetes mellitus over two months after hyperglycemia onset. Diabetes was induced by pancreas resection combined with intraarterial alloxan infusion. Modified isoelectrofocusing in PAAG plates was used for glycosylated hemoglobin measurements. Its concentration was found to increase by 55% 4 weeks after diabetes development and to persist growing, making up 183% of its initial level in 2 months. The suggested method of isoelectric focussing in PAAG plates may be used to monitor glycemia in diabetes.
Askerova
T A
TA
Velikhanova
D M
DM
Gadzhiev
A N
AN
D000180:Acrylic Resins; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000175:diagnosis*; D004285:Dogs; D005260:Female; D005782:Gels; D006442:Glycated Hemoglobin / Q000032:analysis*; D007525:Isoelectric Focusing; D008297:Male
D000180:Acrylic Resins; D005782:Gels; D006442:Glycated Hemoglobin; C016680:polyacrylamide gels
Diagnostika éksperimental'nogo sakharnogo diabeta s pomoshch'iu metoda izoélektricheskogo fokusirovaniia gemoglobina.