title: Platelet release reaction during EDTA-induced platelet agglutinations and inhibition of EDTA-induced platelet agglutination by anti-glycoprotein II b/III a complex monoclonal antibody.
abstract: To characterize the nature of EDTA-induced platelet agglutination, the spontaneous release of beta-thromboglobulin (beta-TG) and platelet factor 4 (PF4) was examined during EDTA-induced platelet agglutinations. A slight release of beta-TG and PF4 was observed when EDTA-anticoagulated whole blood from cases with EDTA-induced platelet agglutination was kept for 60 minutes, whereas a high spontaneous release of these proteins was found from normal blood anticoagulated with EDTA. These findings imply that EDTA-dependent platelet agglutinin may stabilize the platelet membrane surfaces. Secondly, we found that pretreatment of fresh blood with anti-glycoprotein (GP) II b/III a complex monoclonal antibody dramatically reduced EDTA-induced platelet agglutinations. This study indicated that the binding sites of EDTA-dependent antibody might be GP II b/III a complex. The use of an anti-GP II b/III a complex monoclonal antibody may be useful in avoiding analytical errors in some cases with EDTA-induced pseudothrombocytopenia.
authors.affiliation: Blood Transfusion Service, Kobe University Hospital, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Ryo
authors.forename: W
authors.initials: W
authors.lastname: Sugano
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.forename: M
authors.initials: M
authors.lastname: Takada
authors.forename: K
authors.initials: K
authors.lastname: Saigo
authors.forename: M
authors.initials: M
authors.lastname: Hashimoto
authors.forename: N
authors.initials: N
authors.lastname: Yamaguchi
mesh_terms: D000244:Adenosine Diphosphate; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000378:metabolism*; D004492:Edetic Acid; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D010978:Platelet Factor 4 / Q000378:metabolism*; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D001620:beta-Thromboglobulin / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D010980:Platelet Membrane Glycoproteins; D001620:beta-Thromboglobulin; D010978:Platelet Factor 4; D000244:Adenosine Diphosphate; D004492:Edetic Acid