Tissue-specific expression and temporal regulation of the rice glutelin Gt3 gene are conferred by at least two spatially separated cis-regulatory elements.
The rice glutelin Gt3 promoter was fused to a beta-glucuronidase (GUS) reporter gene and its expression evaluated in transgenic tobacco plants. Histochemical analysis revealed that the expression of the introduced Gt3 promoter/GUS (beta-glucuronidase) chimeric gene was confined to endosperm tissue of developing seeds. 5'-promoter deletion analysis revealed that two domains of the Gt3 promoter, -346 to -263 bp (domain I) and -945 to -726 bp (domain II) from the transcriptional start site, were essential for optimum expression of the GUS reporter gene. Removal of 5' sequences upstream of -726 resulted in a reduction in overall promoter activity and a shift in temporal expression from a maximum of 16-20 days after flowering to 24 days. Removal of DNA sequences from the 5' end to -346 yielded a promoter fragment that was still able to confer endosperm-specific expression, although a further deletion to -263 abolished promoter activity. These data suggest that at least two cis-regulatory elements are required for endosperm specificity and temporal regulation of glutelin Gt3 gene expression.
Zhao
Y
Y
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.
Leisy
D J
DJ
Okita
T W
TW
D001483:Base Sequence; D004252:DNA Mutational Analysis; D005786:Gene Expression Regulation; D017343:Genes, Plant / Q000235:genetics*; D017930:Genes, Reporter; D005966:Glucuronidase / Q000032:analysis / Q000235:genetics; D005983:Glutens / Q000096:biosynthesis / Q000235:genetics*; D006651:Histocytochemistry; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012639:Seeds / Q000033:anatomy & histology / Q000201:enzymology; D014018:Tissue Distribution; D014170:Transformation, Genetic
D011993:Recombinant Fusion Proteins; D005983:Glutens; D005966:Glucuronidase