Interleukin-1 stimulates de novo synthesis of mitogen-activated protein kinase in glomerular mesangial cells.
Interleukin-1 (IL-1) stimulates a time- and concentration-dependent mitogen-activated protein (MAP) kinase activation in rat mesangial cells. A rapid increase in activity (maximal at 10 min) is followed by a second persistent level of activity which steadily increases over 24 h. The second peak of MAP kinase activity is paralleled by a marked de novo synthesis of p42 MAP kinase as measured by immunoprecipitation of [35S]methionine-labelled mesangial cells and by a 60% increase in total p42 MAP kinase protein as detected by Western blot analysis. We propose that IL-1 induced de novo synthesis of p42 MAP kinase is important for the multiplicity of long-term actions of this cytokine in renal mesangial cells.
Huwiler
A
A
Department of Pharmacology, Biozentrum, University of Basel, Switzerland.
Pfeilschifter
J
J
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004789:Enzyme Activation; D004790:Enzyme Induction / Q000187:drug effects; D005920:Glomerular Mesangium / Q000166:cytology / Q000201:enzymology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D019950:Mitogen-Activated Protein Kinase 1; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats
D007375:Interleukin-1; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D019950:Mitogen-Activated Protein Kinase 1