[The expression of the main antigens and the growth properties of Yersinia pestis cells on media with different compositions at 35 degrees C].
To improve the technological process of the preparation of vaccines from Y. pestis, the influence of different cultivation conditions on the antigenic composition of bacterial cells was studied. The study of the influence of pH (6.8-7.2), the volume of the culture medium in a flask (25-100 ml), the concentration of amino nitrogen (100-200 mg%) on the growth and phenotypic expression of Y. pestis EV [symbol: see test] antigens at 37 degrees C was studied according to the plan of a complete factor experiment. The data obtained in this study demonstrated the nonlinear dependence of the yield of viable cells on the factors chosen for the process. The presence of F1 and Pst was detected in all tested cultures. The use of the correlation field method made it possible to reveal that the production of antigen pH6 depended on the increased aeration and decreased viability index of the culture. For the production of Pst reverse dependence was revealed.
Gremiakova
T A
TA
Stepanshina
V N
VN
Shuliupin
O K
OK
Negriĭ
V F
VF
Mitsevich
E V
EV
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007114:Immunization; D011817:Rabbits; D012044:Regression Analysis; D013696:Temperature; D013997:Time Factors; D015010:Yersinia pestis / Q000254:growth & development* / Q000276:immunology*
D000942:Antigens, Bacterial; D003470:Culture Media
Ekspressiia osnovnykh antigenov i rostovye svoĭstva kletok Yersinia pestis v sredakh razlichnogo sostava pri 35 gradusov C.