title: The cholinergic inhibition of afterhyperpolarization in rat hippocampus is independent of cAMP-dependent protein kinase.
abstract: The possible involvement of protein kinase A (PKA) in the muscarinic inhibition of the slow afterhyperpolarizing current (IAHP) was investigated in rat hippocampal pyramidal cells. IAHP was recorded using the whole cell method in hippocampal slices, and Rp-cAMPS, a PKA antagonist, was applied intracellularly. The inhibition of IAHP by carbachol was not affected by Rp-cAMPS. In contrast, Rp-cAMPS reduced the cAMP-dependent inhibition of IAHP by norepinephrine. The results show that phosphorylation by PKA does not contribute to the muscarinic effect on IAHP.
authors.affiliation: Department of Psychiatry, Mount Sinai Medical Center, New York, NY 10029.
authors.forename: R D
authors.initials: RD
authors.lastname: Blitzer
authors.forename: T
authors.initials: T
authors.lastname: Wong
authors.forename: R
authors.initials: R
authors.lastname: Nouranifar
authors.forename: E M
authors.initials: EM
authors.lastname: Landau
mesh_terms: D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009638:Norepinephrine / Q000494:pharmacology*; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013873:Thionucleotides / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D013873:Thionucleotides; C016957:adenosine-3',5'-cyclic phosphorothioate; D002217:Carbachol; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D009638:Norepinephrine