Preparation of a DNA matrix via an electrochemically directed copolymerization of pyrrole and oligonucleotides bearing a pyrrole group.
A new methodology for the preparation of addressed DNA matrices is described. The process includes an electrochemically directed copolymerization of pyrrole and oligonucleotides bearing on their 5' end a pyrrole moiety introduced by phosphoramidite chemistry. The electro-controlled synthesis of the copolymer (poly-pyrrole) gives, in one step, a solid conducting film deposited on the surface of an electrode. The resulting polymer consists of pyrrole chains bearing covalently linked oligonucleotide. The polymer growth is limited to the electrode surface, so that it is possible to prepare a DNA matrix on a multiple electrode device by successive copolymerizations. A support bearing four oligonucleotides was used to detect three ras mutations on a synthetic DNA fragment.
Livache
T
T
CIS Bio International, Laboratoire des Sondes Moléculaires, Gif-sur-Yvette, France.
Roget
A
A
Dejean
E
E
Barthet
C
C
Bidan
G
G
Téoule
R
R
D000577:Amides / Q000138:chemical synthesis; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D011905:Genes, ras; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010756:Phosphoric Acids / Q000138:chemical synthesis; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000138:chemical synthesis*
D000577:Amides; D009841:Oligonucleotides; D010756:Phosphoric Acids; D011108:Polymers; D011758:Pyrroles; D004247:DNA; C011067:phosphoramidic acid