[Serodiagnosis of Chlamydia trachomatis and Chlamydia pneumoniae. Evaluation comparing 2 methods: microimmunofluorescence and ELISA. A study of 216 Congolese sera].
Our purpose was to compare two assays for C. trachomatis and C. pneumoniae serology: immunofluorescence and ELISA. With both assays, we tested 216 sera from two populations: risk population of STD (sexually transmitted disease) (women) and a control population "blood donors". Positives percentages of each population were compared by two assays: 87% in MIF and 76% in ELISA (women population); 64.8% in MIF and 53.7% in ELISA (blood donors population). Results were not significantly different. In comparison with ELISA: MIF had a sensitivity of 85% and a specificity of 93%. In comparison with MIF: ELISA had a sensitivity of 98% and a specificity of 48%; agreement between MIF and ELISA in women population and blood donors population is respectively: 88.9% and 78.8%. The discrepancy between both methods is 11.1% in women group and 21.2% in blood donors group. The correlated coefficient (r) between MIF and ELISA was ranged between 0.507 and 0.778 (P < 0.001). Both assays have honourable performance, but no superimposable.
Biendo
M
M
Laboratoire de Bactériologie, CHU, Amiens.
Orfila
J
J
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial; D001782:Blood Donors; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000276:immunology; D002692:Chlamydia trachomatis / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D005831:Genital Diseases, Female / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007247:Infertility, Female / Q000175:diagnosis / Q000382:microbiology; D008875:Middle Aged; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis; D012698:Serologic Tests
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G
Sérodiagnostic de Chlamydia trachomatis et de Chlamydia pneumoniae. Evaluation par comparaison de deux méthodes: microimmunofluorescence et ELISA. Etude de 216 sérums congolais.