Application of poly(L-lactic acid) particles for the suppression of genetic resistance to bone marrow allografts by reticuloendothelial system-blockade.
We have recently successfully achieved bone marrow allografts by reticuloendothelial system (RES)-blockade. Long-term survival of the recipient mice after BMT was achieved without apparent evidence of graft-versus-host disease (GVHD), although T-cells were not depleted from donor marrow cells. Hemopoietic system of these recipient mice was replaced by that of donor origin [7]. As a first step to the application of this technique for human patients, we tested poly(L-lactic acid) (PLLA) particles as biodegradable particles. PLLA particles were phagocytized by macrophages when injected intravenously, and degraded slowly. PLLA has already been in clinical use as a bioresorbable suture material and matrix for drug release. We found that administration of PLLA prior to bone marrow transplantation resulted in a successful take of the allografts. In addition, PLLA was found not to show any antigenicity in normal mice.
Kadowaki
S
S
Department of Biology, Faculty of Science, Niigata University, Japan.
Sugimoto
K
K
Tsurumaki
Y
Y
Tabata
Y
Y
Ikada
Y
Y
Fujita
J
J
Mori
K J
KJ
D000818:Animals; D000906:Antibodies / Q000032:analysis; D016026:Bone Marrow Transplantation / Q000379:methods*; D007113:Immunity, Innate / Q000235:genetics; D007773:Lactates / Q000494:pharmacology* / Q000627:therapeutic use; D019344:Lactic Acid; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D012157:Mononuclear Phagocyte System / Q000187:drug effects*; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology* / Q000627:therapeutic use; D014964:X-Ray Therapy
D000906:Antibodies; D007773:Lactates; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)