DNA fingerprint analysis of Dahl-Iwai salt-sensitive rats (S) and salt-resistant rats (R).
Using DNA fingerprint analyses, extensive molecular heterogeneity has been found between spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY), suggesting a doubtful value of simply comparing these rats in the study of the pathogenesis of genetic hypertension. Therefore, we evaluated the genetic similarity between Dahl salt-sensitive and salt-resistant rats newly inbred by Dr.Iwai (Dahl-Iwai S and R rats), by use of DNA fingerprint analysis. Fingerprint patterns were generated by probing Hinf I- or Alu I-digested DNA with an oligonucleotide corresponded to the tandem repeat sequence of the human myoglobin 33.6 minisatellite. These fingerprint patterns were same within each strain. S and R rts shared 82 percent of the bands in Hinf I-digested DNA and 93 percent of those in Alu I-digested DNA. Although Dahl-Iwai S and R rats are more closely related than SHR and WKY rats, multiple genetic differences still exist between these Dahl strains.
Yamaguchi
S
S
Second Department of Internal Medicine, Yokohama City University School of Medicine, Japan.
Umemura
S
S
Iwamoto
T
T
Kobayashi
S
S
Tamura
K
K
Takagi
N
N
Ishii
M
M
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000235:genetics; D016172:DNA Fingerprinting; D004351:Drug Resistance; D014644:Genetic Variation; D006973:Hypertension / Q000139:chemically induced / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000494:pharmacology*
D012965:Sodium Chloride; D004247:DNA