Characterization of the main egg envelope proteins of the sea bass Dicentrarchus labrax L. (Teleostea, Serranidae).
Fish eggs are surrounded by a resistant acellular coat commonly called the chorion or zona radiata. This study characterizes the eggshell proteinaceous content of unfertilized eggs of the sea bass Dicentrarchus labrax, with a view to the preparation of immunogens. Solubilization of the purified eggshells was achieved in 8 M urea followed by one- and two-dimensional gel electrophoresis. Glycoproteins were detected using concanavalin-A in one and two-dimensional gels, and the principal glycoproteins had a molecular weight of 47 kDa and 170 kDa. Partial purification of a few polypeptides in the 45 kDa to 55 kDa range was achieved by gel filtration chromatography. Although whole eggshells were relatively insoluble even in 8 M urea, partial purification of these polypeptides enable them to dissolve completely in solutions at low ionic strength.
Scapigliati
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G
Dipartimento di Scienze Ambientali, Università della Tuscia, Viterbo, Italy.
Carcupino
M
M
Taddei
A R
AR
Mazzini
M
M
D000818:Animals; D001492:Bass / Q000378:metabolism*; D002823:Chorion / Q000737:chemistry / Q000648:ultrastructure; D004527:Egg Proteins / Q000737:chemistry / Q000302:isolation & purification*; D004528:Egg Shell / Q000737:chemistry / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight
D004527:Egg Proteins; D006023:Glycoproteins