Surface area of apical and basolateral plasmalemma of epithelial cells of the ductuli efferentes testis of the rat.
Serial sectioning was used to determine the occurrence of ciliated cells, and a morphological technique was used to estimate the relative and absolute surface areas of apical and basolateral membrane of the epithelial cells lining the ductuli efferentes of the rat. It was found that the ciliated cells constitute 15% of the epithelial cells and occur as groups of mainly 1-3 cells which are distributed at random in the duct epithelium. For the non-ciliated cells it was estimated that the formation of microvilli by the apical membrane increased the surface area of that border by a factor of 37-fold. The average surface density of the basolateral membrane was 76% the surface density of the apical membrane. However, there was a 3-fold increase in surface density along the apical-basal axis of the basolateral plasmalemma. In the Discussion, the ductuli efferentes are compared to their homologue, the proximal tubules of the kidney, in the rates of fluid transport and membrane adaptations of their epithelium.
Wang
S
S
Department of Biological Sciences, University of Newcastle, NSW, Australia.
Jones
R C
RC
Clulow
J
J
D000818:Animals; D001826:Body Fluids / Q000502:physiology; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002923:Cilia / Q000648:ultrastructure; D004848:Epithelium / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013737:Testis / Q000201:enzymology / Q000502:physiology / Q000648:ultrastructure*
D000254:Sodium-Potassium-Exchanging ATPase