Aphidicolin induces myogenic differentiation in the human rhabdomyosarcoma cell line KFR.
The effects of aphidicolin, a specific inhibitor of DNA polymerase alpha, on cell growth, DNA synthesis and myogenic differentiation in the human alveolar rhabdomyosarcoma cell line KFR were studied. The treatment with aphidicolin at 5 x 10(-6) M concentration, which completely inhibited DNA synthesis and cell growth, induced morphological differentiation of small mononuclear cells to elongated, multinucleated (myotube-like) structures. The morphological differentiation was accompanied by the expression of skeletal muscle myosin; about 30% myosin-positive cells were observed after 14 days of treatment, compared to 2.3% in untreated cultures. The results showed that aphidicolin induces differentiation of human rhabdomyosarcoma cells and that multinucleated myotube-like elements may develop simply by cell fusion without cell division and DNA synthesis.
Cinatl
J
J
Centre of Hygiene, J. W. Goethe-University, Frankfurt a. M., Fed. Rep. of Germany.
Cinatl
J
J
Driever
P H
PH
Rabenau
H
H
Novak
M
M
Stefanik
M
M
Kornhuber
B
B
Doerr
H W
HW
D016590:Aphidicolin / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D009132:Muscles / Q000166:cytology*; D009218:Myosins / Q000096:biosynthesis; D018232:Rhabdomyosarcoma, Alveolar / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
D016590:Aphidicolin; D009218:Myosins