Isolation of Serratia marcescens mutants which could overproduce and excrete Escherichia coli alkaline phosphatase and beta-lactamase.
Serratia marcescens mutants, which excrete Escherichia coli alkaline phosphatase (APase) encoded by the plasmid-bearing phoA gene, were isolated after mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine. These mutants produced two to four times as much APase as did the parent strain under a phosphate-limiting condition, and more than 70% of the enzyme was released into the culture medium. In addition, overproduction and excretion of beta-lactamase was observed in these mutants.
Asdornnithee
S
S
Department of Biotechnology, College of Agriculture, Ehime University, Japan.
Akiyama
K
K
Takata
R
R
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D012706:Serratia marcescens / Q000201:enzymology* / Q000235:genetics; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
D000469:Alkaline Phosphatase; D001618:beta-Lactamases