Interaction between zinc(II)-phthalocyanine-containing liposomes and human low density lipoprotein.
The interaction of human low density lipoprotein (LDL) and small unilamellar liposomes containing the photosensitiser zinc(II)-phthalocyanine (Zn-Pc) was studied in vitro to determine if Zn-Pc could be directly incorporated into the lipoprotein in the absence of other serum components. Incubation of LDL with increasing concentrations of liposomes resulted in a progressive increase in the net negative charge of LDL as determined by agarose gel electrophoresis and both Zn-Pc and liposomal phospholipid were incorporated into the modified LDL particles. Gel chromatography experiments indicated an increase in the molecular mass of modified LDL and immunoaffinity chromatography provided evidence that apoprotein B epitopes on modified LDL were unable to bind to antibody. The study indicated that the liposomal components could be selectively incorporated into LDL by a process that did not appear to involve either aggregation or fusion of particles.
Versluis
A J
AJ
Exploratory Liposome Technology, CIBA-Geigy Pharmaceuticals, Horsham, West Sussex, UK.
Rensen
P C
PC
Kuipers
M E
ME
Love
W G
WG
Taylor
P W
PW
D002250:Carbon Radioisotopes; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007211:Indoles; D054833:Isoindoles; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D008081:Liposomes; D009942:Organometallic Compounds; D010713:Phosphatidylcholines; D015032:Zinc; D017967:Zinc Compounds
D002250:Carbon Radioisotopes; D007211:Indoles; D054833:Isoindoles; D008077:Lipoproteins, LDL; D008081:Liposomes; D009942:Organometallic Compounds; D010713:Phosphatidylcholines; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; C017251:1,2-oleoylphosphatidylcholine; D015032:Zinc; C028694:1-palmitoyl-2-oleoylphosphatidylcholine