title: Identification of two bone morphogenetic protein type I receptors in Drosophila and evidence that Brk25D is a decapentaplegic receptor.
abstract: Drosophila sequences at chromosomal positions 25D (Brk25D) and 43E (Brk43E) are similar to the TGF beta type I receptor serine/threonine kinases and are expressed broadly during embryogenesis. Brk25D binds dpp protein and bone morphogenetic protein 2 with high affinity. Mutations affecting Brk25D map to the gene thick veins and block the expression of two decapentaplegic-responsive (dpp-responsive) genes, dpp and labial, in the embryonic midgut. Defects in Brk25D receptor function combined with reduced expression of dpp ligand produce mutant phenotypes in the embryo and adult. Brk43E is the product of the gene saxophone, which also interacts with dpp. We conclude that dpp signaling in vivo is mediated by at least two receptors, Brk25D and Brk43E.
authors.affiliation: McArdle Laboratory for Cancer Research, Medical School, University of Wisconsin, Madison 53706.
authors.forename: A
authors.initials: A
authors.lastname: Penton
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: K
authors.initials: K
authors.lastname: Staehling-Hampton
authors.forename: J L
authors.initials: JL
authors.lastname: Wrana
authors.forename: L
authors.initials: L
authors.lastname: Attisano
authors.forename: J
authors.initials: J
authors.lastname: Szidonya
authors.forename: J A
authors.initials: JA
authors.lastname: Cassill
authors.forename: J
authors.initials: J
authors.lastname: Massagué
authors.forename: F M
authors.initials: FM
authors.lastname: Hoffmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019485:Bone Morphogenetic Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D005786:Gene Expression Regulation / Q000502:physiology; D017344:Genes, Insect / Q000235:genetics*; D007301:Insect Hormones / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018125:Receptors, Transforming Growth Factor beta / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics; D016212:Transforming Growth Factor beta
chemical_list: D019485:Bone Morphogenetic Proteins; D018076:DNA, Complementary; D029721:Drosophila Proteins; D007301:Insect Hormones; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; C064196:dpp protein, Drosophila; C086338:sax protein, Drosophila; C090169:tkv protein, Drosophila; D017346:Protein Serine-Threonine Kinases