The Rhodobacter capsulatus glnB gene is regulated by NtrC at tandem rpoN-independent promoters.
The protein encoded by glnB of Rhodobacter capsulatus is part of a nitrogen-sensing cascade which regulates the expression of nitrogen fixation genes (nif). The expression of glnB was studied by using lacZ fusions, primer extension analysis, and in vitro DNase I footprinting. Our results suggest that glnB is transcribed from two promoters, one of which requires the R. capsulatus ntrC gene but is rpoN independent. Another promoter upstream of glnB is repressed by NtrC; purified R. capsulatus NtrC binds to sites that overlap this distal promoter region.
Foster-Hartnett
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Department of Biology, Washington University, St. Louis, Missouri.
Kranz
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D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics*; D051922:PII Nitrogen Regulatory Proteins; D011401:Promoter Regions, Genetic; D051877:RNA Polymerase Sigma 54; D012333:RNA, Messenger / Q000235:genetics; D016354:Rhodobacter capsulatus / Q000235:genetics*; D012808:Sigma Factor / Q000235:genetics; D015534:Trans-Activators; D014157:Transcription Factors; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D051922:PII Nitrogen Regulatory Proteins; D012333:RNA, Messenger; D012808:Sigma Factor; D015534:Trans-Activators; D014157:Transcription Factors; C404143:PIID regulatory protein, Bacteria; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54