Energy metabolism of reticulocytes: two different sources of energy for Na+K(+)-ATPase activity.
Total energy production in rabbit reticulocytes amounted to 136.52 +/- 6.50 mumol ATP h-1 ml-1 of reticulocytes: 88.3 per cent was provided by oxidative phosphorylation, whereas only 11.7 per cent by aerobic glycolysis. Na+K(+)-ATPase accounted for 23 per cent, i.e. 27.65 +/- 2.55 mumol ATP h-1ml-1 of reticulocytes, in the overall energy consumption in reticulocytes of rabbits. Under basal conditions ATP for Na+K(+)-ATPase activity was derived exclusively from oxidative phosphorylation. However, when the activity of Na+K(+)-ATPase was increased due to the stimulation of adenylate cyclase by (-)-isoprenaline, the additional energy required was provided by aerobic glycolysis. These results indicate that two different compartments, one cytosolic and the other mitochondrial, provide energy for Na+K(+)-ATPase activity in reticulocytes.
Kostić
M M
MM
Institute of Physiology, Faculty of Medicine, University of Kragujevac, Yugoslavia.
Zivković
R V
RV
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D007545:Isoproterenol / Q000494:pharmacology; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D010042:Ouabain / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D007773:Lactates; D019344:Lactic Acid; D010042:Ouabain; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; D007545:Isoproterenol