The distribution of intracellular calcium concentration in isolated single fibres of mouse skeletal muscle during fatiguing stimulation.
Fluorescence microscopy has been used to examine the distribution of intracellular calcium concentration ([Ca2+]i) in isolated single fibres from the mouse flexor brevis muscle during fatiguing stimulation. Under control conditions there was a virtually uniform distribution of [Ca2+]i in fura-2 loaded fibres either at rest or during short (0.35 s, 100 Hz) tetani. Fatigue produced by repeated short tetani was accompanied by an early rise, followed by a marked fall, in tetanic [Ca2+]i. Throughout the period of fatiguing stimulation the distribution of [Ca2+]i remained uniform with no detectable gradients observed. In contrast, when fatigue was produced by continuous 100 Hz stimulation, a small gradient of [Ca2+]i developed across the fibre with the [Ca2+]i in the centre of the fibre lower than that at the edge of the fibre. This gradient was apparent after 1.7 s, persisted for at least 11 s and was superimposed on a rise followed by a fall in spatially averaged [Ca2+]i. Reduction of the extracellular Na+ to 50% caused reduced force production and a reduced [Ca2+]i in the centre of the fibre. To assess the contribution of reduced response of the myofibrillar proteins to [Ca2+]i during continuous tetani, the relation between [Ca2+]i and force throughout the long tetanus was compared with that obtained in short, unfatigued tetani. These results show that in long tetani, reduced tetanic [Ca2+]i and reduced responsiveness of the myofibrillar proteins to [Ca2+]i each make important contributions to the decline of force, whereas the gradients of [Ca2+]i make only a small contribution.
Duty
S
S
Department of Physiology, University of Sydney, Australia.
Allen
D G
DG
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004558:Electric Stimulation; D016257:Fura-2; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism*; D008297:Male; D051379:Mice; D009119:Muscle Contraction; D009132:Muscles / Q000378:metabolism*; D009994:Osmolar Concentration; D013997:Time Factors; D014018:Tissue Distribution
D002118:Calcium; D016257:Fura-2