title: Influence of thiofedrine on platelet aggregation, intraplatelet cyclic cAMP and malondialdehyde in rats.
abstract: Platelet aggregation and cyclic adenosine monophosphate (cAMP) production were studied by turbidimetry and competitive protein binding assay, respectively, in rats. Thiofedrine (Thi) significantly inhibited adenosine diphosphate (ADP)-induced and thrombin-induced platelet aggregation in vitro, with IC50 values of 0.56 and 0.16 mmol/l, respectively. In vivo, Thi 1.25-5.0 mg/kg i.v. significantly inhibited ADP-induced platelet aggregation at rate of 17.1-40.3%. Thi caused a dose-dependent increase in cAMP levels in rat washed platelets. Malondialdehyde (MDA) levels in rat platelets were measured by colorimetry. Thi had an inhibitory effect on thrombin-induced platelet MDA production. The results suggest that the antiaggregatory action of Thi may be related to metabolism of arachidonic acid (AA) and elevation of cAMP levels.
authors.affiliation: Department of Pharmacology, Inner Mongolia Medical College, Huhehot, China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Qu
authors.forename: X X
authors.initials: XX
authors.lastname: Li
mesh_terms: D000244:Adenosine Diphosphate / Q000037:antagonists & inhibitors; D000818:Animals; D000991:Antithrombins / Q000494:pharmacology*; D001667:Binding, Competitive; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D003124:Colorimetry; D000242:Cyclic AMP / Q000096:biosynthesis*; D005260:Female; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D009391:Nephelometry and Turbidimetry; D010099:Oxyfedrine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013917:Thrombin / Q000502:physiology
chemical_list: D000991:Antithrombins; D010975:Platelet Aggregation Inhibitors; C088651:thiofedrine; D008315:Malondialdehyde; D000244:Adenosine Diphosphate; D010099:Oxyfedrine; D000242:Cyclic AMP; D013917:Thrombin