Accumulation of macrophages in the arterial vessel wall following experimental balloon angioplasty.
Smooth muscle cell (SMC) proliferation is a key event in the development of restenosis after balloon angioplasty, and it is thought that macrophages play an important role in the complex process of activation of SMCs after vascular injury induced by balloon angioplasty. The study was designed to determine the time course of the accumulation of macrophages in the intimal layer following experimental balloon angioplasty. To determine the extent and time course of the accumulation of macrophages after experimental balloon angioplasty, an intimal atheroma was produced by repeated weak electrical stimulation of the right carotid artery of 45 male New Zealand White rabbits. Additionally, the animals received an 0.5% cholesterol diet during the 28 days of plaque development. Transluminal balloon angioplasty was subsequently performed. At 3, 7, 14, 21, 28 and 42 days after balloon treatment the vessels from at least five animals from each group were excised and analysed for the presence of macrophages using immunocytochemical techniques. In one group of five animals plaque development occurred without subsequent balloon angioplasty; the animals were killed after 21 days (sham group). SMCs were identified by immunohistological staining of alpha-actin. Intimal thickening increased after dilatation from 137 +/- 62 microns (control group without balloon treatment) to 244 +/- 47 microns in the 42 days after angioplasty (P < 0.05). The percentage of macrophages in the intimal layer displayed a significant increase (P < 0.01) at 14 days after angioplasty (9.1 +/- 4.3% vs 2.0 +/- 1.7% in the control group).(ABSTRACT TRUNCATED AT 250 WORDS)
Hanke
H
H
Department of Medicine, University of Tübingen, Germany.
Hassenstein
S
S
Ulmer
A
A
Kamenz
J
J
Oberhoff
M
M
Haase
K K
KK
Baumbach
A
A
Gown
A M
AM
Karsch
K R
KR
D000800:Angioplasty, Balloon; D000818:Animals; D002339:Carotid Arteries / Q000473:pathology*; D002340:Carotid Artery Diseases / Q000473:pathology* / Q000628:therapy*; D020212:Carotid Artery Injuries; D002455:Cell Division; D002784:Cholesterol / Q000097:blood; D002537:Intracranial Arteriosclerosis / Q000473:pathology* / Q000628:therapy*; D008264:Macrophages / Q000473:pathology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000293:injuries / Q000473:pathology*; D011817:Rabbits; D012008:Recurrence; D013997:Time Factors; D017539:Tunica Intima / Q000293:injuries / Q000473:pathology*
D002784:Cholesterol