title: Comparative analysis of three antifungal susceptibility test methods against prospectively collected Candida species.
abstract: We performed antifungal susceptibility tests with cilofungin (LY121019), amphotericin B, and flucytosine against 38 strains of yeasts from patients with esophagitis or fungemia either before, during, or after treatment with cilofungin. Tests were performed using a macrobroth dilution method similar to that proposed by the National Committee for Clinical Laboratory Standards (M27-P) and two microbroth methods. For cilofungin and amphotericin B, minimum inhibitory concentrations from microbroth tests using Antibiotic Medium 3 (AM3) were systematically lower than results from the other two methods that utilized RPMI-1640 medium (RPMI). AM3 did not provide any greater degree of in vitro correlation with clinical results than did RPMI. We conclude that cilofungin and possibly other congeners of the echinocandin class of antifungal agents can effectively be studied using the proposed National Committee for Clinical Laboratory Standards method.
authors.affiliation: Medical Service, Veterans Administration Medical Center, Tucson, AZ 85723.
authors.forename: K M
authors.initials: KM
authors.lastname: Villareal
authors.forename: R A
authors.initials: RA
authors.lastname: Cook
authors.forename: J N
authors.initials: JN
authors.lastname: Galgiani
authors.forename: R P
authors.initials: RP
authors.lastname: Wenzel
authors.forename: P G
authors.initials: PG
authors.lastname: Pappas
authors.forename: J C
authors.initials: JC
authors.lastname: Pottage
authors.forename: H A
authors.initials: HA
authors.lastname: Gallis
authors.forename: L R
authors.initials: LR
authors.lastname: Crane
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D002177:Candidiasis / Q000382:microbiology; D004352:Drug Resistance, Microbial; D054714:Echinocandins; D004941:Esophagitis / Q000382:microbiology; D005069:Evaluation Studies as Topic; D005437:Flucytosine / Q000494:pharmacology*; D016469:Fungemia / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D011446:Prospective Studies
chemical_list: D054714:Echinocandins; D010456:Peptides, Cyclic; D000666:Amphotericin B; C042101:cilofungin; D005437:Flucytosine