title: Differences in adhesion and collagen gel contraction between fibroblasts from various types of odontogenic cyst.
abstract: Fibroblasts were grown from explants of normal gingiva and foreskin, and from walls of dentigerous, radicular and residual cysts, as well as keratocysts of basal-cell naevus syndrome and non-syndrome origin. Dentigerous-cyst fibroblast adhesion to poly-L-lysine-coated glass was unaffected by all adhesion-related glycoproteins. Chondroitin sulphate, fibronectin and heparan sulphate enhanced attachment of all other fibroblasts. Chondroitin-sulphate and fibronectin-enhanced adhesion was blocked by an arg-gly-asp peptide. Fibronectin, chondroitin sulphate and laminin all promoted collagen lattice contraction using normal gingival fibroblasts and low-serum media. Fibronectin had a greater effect than chondroitin sulphate and laminin. In media with standard serum, all cyst fibroblast lines examined demonstrated similar gel contraction curves with the exception of dentigerous cyst-derived fibroblasts, which contracted at decreased rates. Suppression of gel contraction was seen with dentigerous-cyst fibroblasts with all extracellular matrix glycoproteins and low serum. Dentigerous-cyst fibroblast attachment to glass and behaviour in gel lattices suggest that these cells express different functional attachment factors from other cyst fibroblast types.
authors.affiliation: Division of Dental Surgery, Leeds Dental Institute, U.K.
authors.forename: A S
authors.initials: AS
authors.lastname: High
authors.forename: P A
authors.initials: PA
authors.lastname: Robinson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002448:Cell Adhesion; D002478:Cells, Cultured; D002675:Child, Preschool; D003094:Collagen / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology*; D006023:Glycoproteins / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009807:Odontogenic Cysts / Q000473:pathology / Q000503:physiopathology*
chemical_list: D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D003094:Collagen