[The use of modified polystyrene plates in solid-phase immunoenzyme analysis for the diagnosis of infections].
The practical aspects of the theory of the adsorption of bioligands on the plastic surface have been considered and discussed. The possibility of standardizing polystyrene plates, as well as obtaining plates with the predetermined level of adsorption activity with respect to given antigens (antibodies), has been shown with the use of the patented technology for the modification of polystyrene plates. The data on the adsorption of antigens used as an experimental model (human and rabbit immunoglobulin fractions) are presented, and an increase in adsorption activity has been shown with the use of modified plates. For practical confirmation, some examples of using these plates in actual assay systems intended for the evaluation of HBsAg carriership and antibodies to HIV are given. A considerable increase (2- to 10-fold) of the signal from positive samples has been shown to occur, the signal from negative samples remaining practically unchanged.
Meshandin
A G
AG
Vaneeva
L I
LI
Agapkina
N P
NP
Mironova
V V
VV
Dargeeva
T A
TA
D000327:Adsorption; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D003141:Communicable Diseases / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D015483:HIV Antibodies / Q000097:blood; D015497:HIV-1 / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000097:blood; D008459:Measles virus / Q000276:immunology; D011137:Polystyrenes; D011817:Rabbits
D000914:Antibodies, Viral; D015483:HIV Antibodies; D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G; D011137:Polystyrenes
Ispol'zovanie modifitsirovannykh polistirolovykh planshetov v tverdofaznom immunofermentnom analize dlia diagnostiki nekotorykh infektsiĭ.