A method for protein assay in Laemmli buffer.
A simple, cheap, and rapid assay for soluble and membrane-bound protein solubilized in Laemmli sodium dodecyl sulfate sample buffer is described. Following the addition of trichloroacetic acid to a final concentration of 24%, the induced turbidity was measured at 570 nm in a microplate reader, after an incubation period of approximately 10-30 min. The assay is suitable for measuring bovine serum albumin at a concentration of 15 to 500 micrograms/ml in Laemmli buffer, corresponding to 2 to 75 micrograms protein per well on the microtiter plate. Other nonproteinaceous macromolecules in the sample may introduce an error.
Karlsson
J O
JO
Department of Anatomy and Cell Biology, University of Göteborg, Sweden.
Ostwald
K
K
Kåbjörn
C
C
Andersson
M
M
D000818:Animals; D001923:Brain Chemistry; D007202:Indicators and Reagents; D009391:Nephelometry and Turbidimetry / Q000379:methods; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D014325:Tromethamine
D007202:Indicators and Reagents; C088816:Laemmli buffer; D009419:Nerve Tissue Proteins; D011506:Proteins; D014325:Tromethamine; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate