title: Characterization of phospholipidic components of the natural pulmonary surfactant Curosurf.
abstract: The phospholipidic classes of the natural pulmonary surfactant Curosurf were separated by bidimensional TLC and isolated. Quantitative analysis of each class was made by colorimetric determination of phosphorus. FD/MS and NH3-CI/MS were used to identify individual components. GC was used for qualitative/quantitative analysis of fatty acid moieties as methyl esters, using internal standards, by comparison with authentic samples.
authors.affiliation: Chemical and Biopharmaceutical Department, Chiesi Farmaceutici SpA, Parma, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Redenti
authors.forename: T
authors.initials: T
authors.lastname: Peveri
authors.forename: P
authors.initials: P
authors.lastname: Ventura
authors.forename: M
authors.initials: M
authors.lastname: Zanol
authors.forename: A
authors.initials: A
authors.lastname: Selva
mesh_terms: D000818:Animals; D001688:Biological Products; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D005227:Fatty Acids / Q000032:analysis; D013058:Mass Spectrometry; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000737:chemistry*; D011663:Pulmonary Surfactants / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
chemical_list: D001688:Biological Products; D005227:Fatty Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D011663:Pulmonary Surfactants; C068291:poractant alfa