Detection of infections of the eye with Chlamydia trachomatis by the polymerase chain reaction.
The aim of this study was to test the diagnostic feasibility of the polymerase chain reaction (PCR) for detection of infections with Chlamydia trachomatis in eye swabs from patients with conjunctivitis, and to establish the basic technique of the PCR for epidemiological survey. The results of the PCR were compared with the Mikro Trak immunofluorescence assay (IFA). From 49 specimens of patients with conjunctivitis, 31 were found positive by PCR (63%) and 23 by IFA (47%). On the other hand, in 10 normal eye specimens and 10 non-Chlamydia trachoma conjunctivitis specimens no Chlamydia trachomatis was detected.
Fan
J
J
Beijing Institute of Ophthalmology, Chongwenmen, China.
Zhang
W H
WH
Wu
Y Y
YY
Jing
X Y
XY
Claas
E C
EC
D000907:Antibodies, Bacterial / Q000032:analysis; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000276:immunology; D003228:Conjunctiva / Q000382:microbiology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D014141:Trachoma / Q000175:diagnosis* / Q000382:microbiology
D000907:Antibodies, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial