title: Evolutionary pattern of human respiratory syncytial virus (subgroup A): cocirculating lineages and correlation of genetic and antigenic changes in the G glycoprotein.
abstract: The genetic and antigenic variability of the G glycoproteins from 76 human respiratory syncytial (RS) viruses (subgroup A) isolated during six consecutive epidemics in either Montevideo, Uruguay, or Madrid, Spain, have been analyzed. Genetic diversity was evaluated for all viruses by the RNase A mismatch cleavage method and for selected strains by dideoxy sequencing. The sequences reported here were added to those published for six isolates from Birmingham, United Kingdom, and for two reference strains (A2 and Long), to derive a phylogenetic tree of subgroup A viruses that contained two main branches and several subbranches. During the same epidemic, viruses from different branches were isolated. In addition, closely related viruses were isolated in distant places and in different years. These results illustrate the capacity of the virus to spread worldwide, influencing its mode of evolution. The antigenic analysis of all isolates was carried out with a panel of anti-G monoclonal antibodies that recognized strain-specific (or variable) epitopes. A close correlation between genetic relatedness and antigenic relatedness in the G protein was observed. These results, together with an accumulation of amino acid changes in a major antigenic area of the G glycoprotein, suggest that immune selection may be a factor influencing the generation of RS virus diversity. The pattern of RS virus evolution is thus similar to that described for influenza type B viruses, expect that the level of genetic divergence among the G glycoproteins of RS virus isolates is the highest reported for an RNA virus gene product.
authors.affiliation: Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors.forename: O
authors.initials: O
authors.lastname: García
authors.forename: M
authors.initials: M
authors.lastname: Martín
authors.forename: J
authors.initials: J
authors.lastname: Dopazo
authors.forename: J
authors.initials: J
authors.lastname: Arbiza
authors.forename: S
authors.initials: S
authors.lastname: Frabasile
authors.forename: J
authors.initials: J
authors.lastname: Russi
authors.forename: M
authors.initials: M
authors.lastname: Hortal
authors.forename: P
authors.initials: P
authors.lastname: Perez-Breña
authors.forename: I
authors.initials: I
authors.lastname: Martínez
authors.forename: B
authors.initials: B
authors.lastname: García-Barreno
mesh_terms: D000595:Amino Acid Sequence; D000940:Antigenic Variation; D001483:Base Sequence; D005075:Biological Evolution; D017931:DNA Primers / Q000737:chemistry; D004196:Disease Outbreaks; D005814:Genes, Viral; D015707:HN Protein; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology*; D012136:Respiratory Syncytial Viruses / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013030:Spain; D014583:Uruguay; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D015707:HN Protein; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C116204:attachment protein G