Preparative scale culture of Escherichia coli cells expressing the human immunodeficiency virus type 1 Tat protein.
A procedure leading to a 100-liter fermentor culture of Escherichia coli cells expressing the human immunodeficiency virus type 1 (HIV-1) trans-activator (Tat) protein is described. The effects of growth temperature and of cell density at the time of induction on the yield of Tat were investigated. Tat was identified by SDS-gel electrophoresis and Western blot. Tat represents approximately 10% of the soluble protein in the cell lysate.
McKenna
M C
MC
Molecular Biology Institute, University of California at Los Angeles 90024.
Muchardt
C
C
Gaynor
R
R
Eisenberg
D
D
D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005285:Fermentation; D015696:Gene Products, tat / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D005813:Genes, Synthetic; D015756:Genes, tat; D015497:HIV-1 / Q000235:genetics*; D007218:Industrial Microbiology / Q000379:methods*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus