The fractionation of human plasma proteins. I. Affinity purification of human complement properdin.
A method was developed for the affinity purification of human complement properdin. The preparation is part of an integrated scheme in which over 20 human plasma proteins can be recovered in a highly purified form. The yield of properdin was 5.9 mg from 3 liters of plasma, amounting to a 28% recovery. The crucial step in the purification was the application of affinity chromatography using C3b-ester-Sepharose.
DiScipio
R G
RG
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
D001798:Blood Proteins / Q000302:isolation & purification; D002266:Carboxymethylcellulose Sodium; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003179:Complement C3b / Q000378:metabolism; D003170:Complement Pathway, Alternative; D006801:Humans; D011092:Polyethylene Glycols; D011414:Properdin / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding
D001798:Blood Proteins; D011414:Properdin; D011092:Polyethylene Glycols; D003179:Complement C3b; D002266:Carboxymethylcellulose Sodium