Functional evidence regarding receptor subtypes mediating the actions of native gonadotropin-releasing hormones (GnRH) in goldfish, Carassius auratus.
In goldfish, two native gonadotropin-releasing hormone (GnRH) peptides, salmon GnRH (sGnRH) and chicken-II GnRH (cGnRH-II), stimulate gonadotropin (GTH-II) and growth hormone (GH) secretion. In the present study we tested whether sGnRH and cGnRH-II act through different or the same population of GnRH receptors on GTH and GH cells, using various approaches. A GnRH antagonist [Ac-delta 3-Pro1,4FD-Phe2,D-Trp3,6]sGnRH (analog C) equally inhibited GTH-II release by sGnRH and cGnRH-II. Similarly, [Ac-D(2)Nal1,4Cl-D-Phe2,D-(3)Pal3,6]cGnRH-II (analog N) suppressed GTH-II stimulation by the two GnRH peptides equally. Further, in the continued presence of 100 nM sGnRH, a 100 nM pulse of cGnRH-II failed to cause additional GTH-II release and vice versa. Prolonged exposure of pituitary fragments to 100 nM sGnRH or cGnRH-II resulted in desensitization and reduced the GTH-II response to a subsequent pulse of either sGnRH or cGnRH-II equally. All these results indicate that both sGnRH and cGnRH-II act through the same population of GnRH receptors on gonadotrophs. Similarly, the GH responses obtained with (i) analog N treatment, (ii) pulse challenge to cGnRH-II during prolonged sGnRH treatment and vice versa, and (iii) desensitization studies suggest that both sGnRH and cGnRH-II act through the same population of GnRH receptors on somatotrophs. However, the properties of GnRH receptors on GTH-II and GH cells are different. Analog C stimulated GH release but not GTH-II release. In contrast, [Ac-delta 3-Pro1,4FD-Phe2,D-Trp3,D-Arg6]mGnRH stimulated GTH-II release but weakly suppressed GH release. These results indicate that sGnRH and cGnRH-II act on the same population of receptors on GTH cells and on a functionally distinct population of receptors on GH cells.
Murthy
C K
CK
Department of Zoology, University of Alberta, Edmonton, Canada.
Peter
R E
RE
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006054:Goldfish / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006065:Gonadotropins, Pituitary / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008969:Molecular Sequence Data; D011966:Receptors, LHRH / Q000502:physiology*
D006065:Gonadotropins, Pituitary; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone; C036959:LHRH, Ac-dehydro-Pro(1)-4-F-Phe(2)-Trp(3,6)-; D013006:Growth Hormone; C047717:LHRH, His(5)-Trp(7)-Tyr(8)-