Measurement characteristics of stereological and histochemical methods in the diagnosis of thyroid tumours.
Seventy-two female mice of BALB/c strain were divided into three control and three perchlorate groups. The perchlorate groups drank 1.2% sodium perchlorate solution. After 8 or 32 weeks of the 46-week experiment one control and one perchlorate group were totally irradiated with 0.8 Gy on 5 consecutive days. Ten normal thyroid glands, 10 with hypertrophy and hyperplasia, 8 follicular adenomas and 10 follicular carcinomas were histologically and stereologically analysed. The results showed significant differences in the average nuclear volume (p < 0.05) and in the average number of the silver-stained nucleolar organiser regions (AgNORs) per nucleus (p < 0.005) between the follicular adenomas and the follicular carcinomas. The method of counting Ag-NORs per nucleus was more valid, more accurate and more economical than the method of measuring the average nuclear volume.
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Institute of Histology and Embryology, Faculty of Medicine, Ljubljana, Slovenia.
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D018263:Adenocarcinoma, Follicular / Q000473:pathology*; D000236:Adenoma / Q000473:pathology*; D000818:Animals; D002467:Cell Nucleus / Q000473:pathology*; D003937:Diagnosis, Differential; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009697:Nucleolus Organizer Region / Q000473:pathology*; D013964:Thyroid Neoplasms / Q000473:pathology*