title: Atrial natriuretic peptide immunoreactivity in the eggs of the silkworm Bombyx mori.
abstract: Synthesis and secretion of atrial natriuretic peptides (ANPs) are not confined to the atrium, but are also present in other tissues. Recently, we have found synthesis of ANP in the eggs of several vertebrate animals. The present study was undertaken to determine whether immunoreactive ANP (irANP) is present in the egg of an invertebrate, the silkworm (Bombyx mori L.). The serial dilution curve of egg extracts of silkworm was parallel to the standard curve of atriopeptin III. Analysis of ANP immunoreactivity by gel filtration chromatography and reverse-phase HPLC showed that the major immunoreactivity corresponded to rat proANP. The semipurified irANP of egg extracts produced a dose-dependent relaxation on rat aortic strips, which was blocked by preincubation with anti-ANP antiserum. Therefore, we suggest that ANP is synthesized in the silkworm egg.
authors.affiliation: Department of Physiology, Jeonbug National University Medical School, Republic of Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: H
authors.initials: H
authors.lastname: Ryu
authors.forename: C W
authors.initials: CW
authors.lastname: Kang
authors.forename: S Z
authors.initials: SZ
authors.lastname: Kim
authors.forename: K H
authors.initials: KH
authors.lastname: Seul
authors.forename: K W
authors.initials: KW
authors.lastname: Cho
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000502:physiology; D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000494:pharmacology; D012831:Bombyx; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D010063:Ovum / Q000737:chemistry*; D010446:Peptide Fragments; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects
chemical_list: D010446:Peptide Fragments; C062525:atrial natriuretic factor prohormone (103-126); D009320:Atrial Natriuretic Factor