Synthesis of polymer-coated mixed-functional packing materials for direct analysis of drug-containing serum and plasma by high-performance liquid chromatography.
Silicone polymer-coated mixed-functional (PCMF) silica packing materials were developed for the direct determination of drugs contained in serum or plasma. The new stationary phases were prepared by the following three-step procedure: (1) coating porous silica with a silicone polymer; (2) partial introduction of hydrophobic groups; and (3) introduction of hydrophilic groups. Two PCMFs were synthesized, one having polyoxyethylene groups as hydrophilic groups (PCMF-POE) and the other having oligoglyceryl groups (PCMF-OG). PCMF-POE showed higher recoveries for injected proteins and a greater overall retention for drug molecules than PCMF-OG. PCMF-POE did not show any column deterioration in 500 serum sample injections (10 ml in total).
Kanda
T
T
Shiseido Research Centre, Yokohama, Japan.
Kutsuna
H
H
Ohtsu
Y
Y
Yamaguchi
M
M
D002220:Carbamazepine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D010634:Phenobarbital / Q000097:blood*; D010672:Phenytoin / Q000097:blood*; D011108:Polymers; D012822:Silicon Dioxide
D011108:Polymers; D002220:Carbamazepine; D010672:Phenytoin; D012822:Silicon Dioxide; D010634:Phenobarbital