Expression of beta-amyloid precursor protein gene is developmentally regulated in human muscle fibers in vivo and in vitro.
Regenerating muscle fibers in 25 human muscle biopsies, obtained from patients with a variety of neuromuscular diseases, manifested increased mRNA for the beta-amyloid precursor protein (beta APP) that contains the Kunitz-type protease inhibitor (KPI) motif, whereas adult human muscle fibers were negative in their vast non-extrajunctional region. Aneurally cultured normal human muscle fibers also expressed strong KPI-beta APP mRNA signal, which became significantly down-regulated during muscle differentiation. Our study demonstrates that in human muscle KPI-beta APP mRNA is developmentally regulated, and it suggests that beta APP may play a role in human muscle development.
Sarkozi
E
E
USC Neuromuscular Center, University of Southern California School of Medicine, Los Angeles 90017.
Askanas
V
V
Johnson
S A
SA
McFerrin
J
J
Engel
W K
WK
D000293:Adolescent; D000328:Adult; D000368:Aged; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry / Q000235:genetics*; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D046508:Culture Techniques; D005786:Gene Expression Regulation; D006801:Humans; D008875:Middle Aged; D009132:Muscles / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D010455:Peptides; D010940:Plant Proteins; D012333:RNA, Messenger / Q000378:metabolism; D012038:Regeneration; D014361:Trypsin Inhibitors / Q000737:chemistry
D016564:Amyloid beta-Protein Precursor; C053865:Kunitz-type protease inhibitor, plant; D010455:Peptides; D010940:Plant Proteins; D012333:RNA, Messenger; D014361:Trypsin Inhibitors