Some physicochemical and enzymic properties of selenium-containing abzyme.
We successfully prepared the Se-containing abzyme (Se-abzyme) with glutathione peroxidase (GPX) activity and further studied its physicochemical and enzymic properties and stabilities. Data showed that the isoelectric point of the abzyme was 6.95-7.08, and its molecular weight was 158 KD. The ranges of optimum pH and temperature of the Se-abzyme were wider than the native GPX. The store stability of the abzyme was higher than the native GPX. The Se content in the abzyme was found to be 5 mol Se/mol abzyme by X-ray photoelectron spectrum, and binding constant 1.11 x 10(7)M-1 by using ELISA method. The Se-abzyme was inhibited competitively by dithiobis(2-nitrobenzoic acid) (DTNB), and inhibition constant was determined to be 1.25 x 10(-3)M-1.
Zhu
Z Q
ZQ
National Laboratory of Enzyme Engineering, Jilin University, Changchun, P.R. China.
Ding
L
L
Luo
G M
GM
Liu
Z
Z
Sun
Q A
QA
Yang
T S
TS
Shen
J C
JC
D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008970:Molecular Weight; D009154:Mutation; D012643:Selenium / Q000032:analysis*; D013379:Substrate Specificity; D013696:Temperature
D004798:Enzymes; D005979:Glutathione Peroxidase; D005978:Glutathione; D012643:Selenium