Photosensitization with a chlorin-thiobarbiturate conjugate.
The photobiologic properties of a chlorin-thiobarbiturate conjugate were examined. In addition to the characteristic 670 nm chlorin absorbance, the conjugate exhibited new absorbance bands at 517 and 743 nm, but these were not a part of the fluorescence emission spectra, nor was fluorescence observed at wavelengths > 670 nm. The action spectrum indicated that the long-wavelength absorbance of the conjugate was not involved in phototoxicity in cell culture, and that only irradiation at wavelengths associated with the chlorin moiety yielded a cytotoxic effect. In vivo cytotoxicity elicited by 745 nm irradiation is attributed to thermal effects.
Kessel
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Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.
Morgan
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A
D000818:Animals; D007939:Leukemia L1210 / Q000188:drug therapy; D051379:Mice; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology*; D013858:Thiobarbiturates / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects
D017319:Photosensitizing Agents; D011166:Porphyrins; D013858:Thiobarbiturates; C006969:chlorin; C029684:thiobarbituric acid