Turbidimetric latex agglutination inhibition immunoassay for primidone.
We have developed a quick, highly sensitive immunoassay method for drugs by latex agglutination inhibition. An antiserum against primidone (PRM) was obtained by immunizing rabbits with PRM-bovine serum albumin conjugate. PRM-rabbit serum albumin conjugate sensitized latex was agglutinated with diluted antiserum, and the agglutination was inhibited by free PRM quantitatively. Turbidity of the agglutination suspension was measured by spectrophotometry as absorbance. Larger latex gave higher sensitivity than the smaller, because its agglutination was inhibited more intensely by free PRM. The assay values of this method were correlated well with those obtained by an enzyme immunoassay method.
Kubota
N
N
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Momose
K
K
Maekawa
H
H
Tsubota
N
N
D000818:Animals; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D009391:Nephelometry and Turbidimetry; D010316:Particle Size; D011137:Polystyrenes; D011324:Primidone / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine; D013056:Spectrophotometry, Ultraviolet
D011137:Polystyrenes; D011324:Primidone; D012710:Serum Albumin, Bovine