title: Interaction of carbonyl cyanide 3-chlorophenylhydrazone with cytochrome c oxidase.
abstract: Cytochrome c oxidase binds protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP) with high affinity. There are 1.46 high-affinity binding sites per cytochrome c oxidase for CCCP with dissociation constant 2.7 x 10(-7) mol/l. The bond between the CCCP and cytochrome c oxidase accomplishes through the group on cytochrome c oxidase with pKa 6.64 and is based on the electrostatic interaction. Interaction of CCCP with low-affinity binding sites of cytochrome c oxidase induces the shift of the anion CCCP spectrum to UV-region. The similar effect is characteristic for CCCP interaction with protons. Lipophilic non-dissociated derivative NCH3CCP is not binding to cytochrome c oxidase.
authors.affiliation: Department of Biophysics, Slovak Academy of Sciences, Kosice.
authors.forename: M
authors.initials: M
authors.lastname: Bona
authors.forename: M
authors.initials: M
authors.lastname: Antalík
authors.forename: Z
authors.initials: Z
authors.lastname: Gazová
authors.forename: A
authors.initials: A
authors.lastname: Kuchár
authors.forename: V
authors.initials: V
authors.lastname: Dadák
authors.forename: D
authors.initials: D
authors.lastname: Podhradský
mesh_terms: D001665:Binding Sites; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000031:analogs & derivatives / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D011485:Protein Binding
chemical_list: C089132:4-chlorophenyl-N-methylhydrazonopropanedinitrile; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003576:Electron Transport Complex IV