title: Phylogenetic utility of ubiquitin DNA sequence from 3 marine protist lineages.
abstract: The foraminifera are one of the last major groups of eukaryotes for which no published DNA sequences exist. DNA sequence of ubiquitin repeat units from 5 foraminifera (representing 3 suborders), a diatom, and a dinoflagellate were characterized using the polymerase chain reaction, cloning, and sequencing. The phylogenetic utility of ubiquitin DNA sequence is discussed in reference to a full eukaryote data base. The foraminifera are possibly a polyphyletic group, whereas the phylogenetic placement of the diatom and the dinoflagellate are congruent with existing hypotheses. The lack of a noneukaryote root and limited phylogenetically informative sequence information indicate that polyubiquitin may not be useful for phylogenetic reconstruction of all eukaryotes.
authors.affiliation: Department of Geology and Geophysics, Yale University, New Haven, CT 06511.
authors.forename: C G
authors.initials: CG
authors.lastname: Wray
authors.forename: R
authors.initials: R
authors.lastname: DeSalle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017377:Diatoms / Q000145:classification / Q000235:genetics*; D004141:Dinoflagellida / Q000145:classification / Q000235:genetics*; D056890:Eukaryota / Q000145:classification / Q000235:genetics*; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014452:Ubiquitins / Q000235:genetics*
chemical_list: D014452:Ubiquitins