PCR in a silicon microstructure.
Devices for performing polymerase chain reactions (PCR) have been developed for use with photolithographed silicon. Microchambers capable of holding between 5.0 and 10 microL of PCR reagents were constructed by etching specific areas of rectangular silicon chips (17 x 15 mm), which were then capped with Pyrex glass. These silicon devices (PCRChips), which were etched to depths of 40-80 microns, permitted free flow of fluids in the microchannels and microchambers. Access to the microchambers was through holes in the silicon. Thermal cycling of the PCR reagents was achieved by placing the disposable PCRChip in a small holder containing a computer-controlled Peltier heater-cooler. Successful amplification was demonstrated by electrophoresis of products in agarose gel containing ethidium bromide, and the migration of the product was compared with that obtained in a commercially available thermal cycler. The thermal characteristics of the silicon, coupled with the high surface area to volume ratio in the new devices, are particularly advantageous features for amplification by PCR.
Wilding
P
P
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104.
Shoffner
M A
MA
Kricka
L J
LJ
D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral; D016133:Polymerase Chain Reaction / Q000379:methods*; D012825:Silicon
D004279:DNA, Viral; D012825:Silicon