The robustness of recombination frequency estimates in intercrosses with dominant markers.
The robustness of the maximum likelihood estimates of recombination frequencies has been investigated in double intercrosses with complete dominance at both loci. The robustness was investigated with respect to bias in the recombination frequency estimates due to: (1) limited sample sizes, (2) heterogeneity in recombination frequencies between sexes or among meioses and (3) factors that distort the segregation-misclassification or differential viability. In the coupling phase, the recombination frequency estimates are quite robust with respect to most of the investigated factors. Potentially, the most serious cause of a bias is misclassifications, which tend to increase the recombination frequency estimates. In the repulsion phase, misclassifications are particularly serious, leading to extreme discrepancies between true and observed values. In addition, limited sample size and sex differences in recombination can also bias recombination frequency estimates in repulsion. These effects may pose serious problem in genetic mapping with random amplified polymorphic DNA (RAPD) markers.
Säll
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Department of Genetics, Lund University, Sweden.
Nilsson
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NO
D000818:Animals; D003433:Crosses, Genetic; D005799:Genes, Dominant; D008040:Genetic Linkage; D006801:Humans; D008433:Mathematics; D008957:Models, Genetic; D011110:Polymorphism, Genetic; D011336:Probability; D011995:Recombination, Genetic