title: Identification of bacterial cell-surface virulence determinants with TnphoA.
abstract: Insertion mutagenesis using TnphoA has proved to be a potent device for the creation of easily screened knockout mutations in genes encoding virulence determinants in a variety of pathogenic bacteria. Initial identification of genes with TnphoA directly initiates more sophisticated genetic and biochemical studies on these factors essential to our understanding of bacterial pathogenesis.
authors.affiliation: Department of Biological Sciences, Hopkins Marine Station, Stanford University, Pacific Grove, California 93950.
authors.forename: M R
authors.initials: MR
authors.lastname: Kaufman
authors.forename: R K
authors.initials: RK
authors.lastname: Taylor
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001419:Bacteria / Q000235:genetics / Q000472:pathogenicity*; D001426:Bacterial Proteins / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004285:Dogs; D005822:Genetic Vectors; D007501:Iron / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016254:Mutagenesis, Insertional / Q000379:methods*; D017384:Sequence Deletion; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D007501:Iron; D000469:Alkaline Phosphatase