[A 2-generation fertility study with 1,4-dichlorobenzene in rats].
1,4-Dichlorobenzene (pDCB), dissolved in olive oil, was administered orally to two generations of rats to detect possible effects of the test substance on reproduction (OECD guideline 416). The doses used for the three test substance groups were 30, 90 and 270 mg pDCB per kg body weight. Olive oil was applied to one negative control group. Dose volume was 1 ml per kg body weight for all groups. In both generations the test substance had no effects on: time between beginning of mating and evidence of copulation, time of gestation, fertility index, gestational index, percentage of dams with only dead pups, total number of pups at birth, percentage of pups with positive ear reflex, grasping reflex and orientation reaction, absolute and relative weights of testes, epididymides and ovaries, absolute and relative weights of female kidneys, spleens and livers. In the mid and/or high-dose groups the test substance had an effect on: number of live pups at birth (reduced), number of pups deceased between days of lactation 1 to 4 and 5 to 21 (increased), mean body weight of pups (reduced), erection of ears and opening of eyes (retarded), percentage of pups with positive draw up test (reduced), alterations of the skin (dry, squamous) and ring tails in pups, absolute and relative organ weights of adult males (livers and kidneys: increased; spleens: reduced). Damages to the kidneys in mid- and high-dose groups of both generations are also probably test substance related. At the low dose of 30mg pDCB per kg body weight--equivalent to the actual MAK-value of 450 mg/m3 (exposure by inhalation)--no effects of the test substance were detected on fertility parameters of rats. The next higher dose of 90 mg/kg already caused massive damage in pups. Therefore, a reduction of the actual MAK value should be taken into consideration.
Bornatowicz
N
N
Osterreichisches Forschungszentrum Seibersdorf.
Antes
A
A
Winker
N
N
Hofer
H
H
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002722:Chlorobenzenes / Q000633:toxicity*; D003307:Copulation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005298:Fertility / Q000187:drug effects*; D007774:Lactation / Q000187:drug effects; D008098:Litter Size / Q000187:drug effects; D008297:Male; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D002722:Chlorobenzenes; C018511:4-dichlorobenzene
2-Generationen-Fertilitätsstudie mit 1,4-Dichlorbenzol an Ratten.