title: A PCR-aided transcript titration assay revealing very low expression of a gene at band 3p21 in 33 cells lines derived from all types of lung cancer.
abstract: We have developed a general PCR-based method to quantify the amount of a specific mRNA present in a given cell line or tissue. We applied this quantitative PCR to analyse the expression of D8, a human gene which we recently identified in the chromosomal region 3p21, the common deletion region of lung cancer. Our PCR-aided assay shows that in most lung-cancer-derived cell lines the amount of D8 transcripts is only 2% or less of that in normal lung tissue. The virtual absence of expression may imply some role of the gene in the development of lung cancer.
authors.affiliation: Department of Medical Genetics, University of Groningen, The Netherlands.
authors.forename: K
authors.initials: K
authors.lastname: Kok
authors.forename: A
authors.initials: A
authors.lastname: van den Berg
authors.forename: D L
authors.initials: DL
authors.lastname: Buchhagen
authors.forename: B
authors.initials: B
authors.lastname: Carritt
authors.forename: C H
authors.initials: CH
authors.lastname: Buys
mesh_terms: D001483:Base Sequence; D002893:Chromosomes, Human, Pair 3; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger