Physical association of JAK1 and JAK2 tyrosine kinases with the interleukin 2 receptor beta and gamma chains.
The functional interleukin 2 (IL-2) receptors contain the beta and gamma chains which are necessary for the transduction of cell growth signals. Monoclonal antibodies specific for the beta chain and gamma chain coimmunoprecipitated JAK1 and 114-kDa JAK2 tyrosine kinases, respectively. Tyrosine phosphorylation of JAK1 and JAK2 was induced upon IL-2 stimulation, and IL-2 activated the JAK2 kinase. These results demonstrate that the JAK1 and JAK2 tyrosine kinases are physically associated with the beta chain and gamma chain, respectively, and suggest that regulation of the kinases may be linked to IL-2-induced signal transduction.
Tanaka
N
N
Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
Asao
H
H
Ohbo
K
K
Ishii
N
N
Takeshita
T
T
Nakamura
M
M
Sasaki
H
H
Sugamura
K
K
D001483:Base Sequence; D002460:Cell Line; D004247:DNA; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D053613:Janus Kinase 1; D053614:Janus Kinase 2; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D007376:Interleukin-2; D011518:Proto-Oncogene Proteins; D015375:Receptors, Interleukin-2; D014443:Tyrosine; D004247:DNA; D011505:Protein-Tyrosine Kinases; C507904:JAK1 protein, human; C507924:JAK2 protein, human; D053613:Janus Kinase 1; D053614:Janus Kinase 2