title: Mutations in the Bordetella pertussis bvgS gene that confer altered expression of the fhaB gene in Escherichia coli.
abstract: The bvg locus of Bordetella pertussis, required for coordinate regulation of virulence genes in response to environmental signals, encodes two proteins, BvgS and BvgA, that belong to the bacterial two-component signal transduction systems. We have isolated spontaneous mutations of the bvg locus in Escherichia coli and analyzed their effects on the expression of fhaB::lacZY transcriptional fusions. The mutations, localized in the linker and transmitter domain of BvgS, result in increased activation of fhaB and/or in insensitivity to a modulating agent, nicotinic acid.
authors.affiliation: Unité de Biochimie des Régulations Cellulaires, Institut Pasteur, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Goyard
authors.forename: J
authors.initials: J
authors.lastname: Bellalou
authors.forename: H
authors.initials: H
authors.lastname: Mireau
authors.forename: A
authors.initials: A
authors.lastname: Ullmann
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001886:Bordetella pertussis / Q000235:genetics*; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006388:Hemagglutinins / Q000235:genetics; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000502:physiology; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D006388:Hemagglutinins; D010750:Phosphoproteins; D014157:Transcription Factors; C080662:bvgS protein, Bordetella pertussis