title: Determination of sterol content in different food samples by capillary gas chromatography.
abstract: An accurate method for the determination of sterols by capillary gas chromatography was developed and applied to the analysis of food. The procedure includes the following steps: dichloromethane-methanol (2:1, v/v) lipid extraction, saponification at 80 degrees C and separation of the unsaponifiable matter with cyclohexane, derivatization to form trimethylsilyl ethers and gas chromatography using 5 alpha-cholestane as the internal standard. The method shows good accuracy, precision and sensitivity and is suitable for the determination of sterols in food.
authors.affiliation: Department de Ciències Fisiològiques i de la Nutrició, Facultat de Farmàcia, Universitat de Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez-Palmero
authors.forename: S
authors.initials: S
authors.lastname: de la Presa-Owens
authors.forename: A I
authors.initials: AI
authors.lastname: Castellote-Bargallo
authors.forename: M C
authors.initials: MC
authors.lastname: López Sabater
authors.forename: M
authors.initials: M
authors.lastname: Rivero-Urgell
authors.forename: M C
authors.initials: MC
authors.lastname: de la Torre-Boronat
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D005399:Fishes; D005504:Food Analysis; D006801:Humans; D011200:Poultry; D013261:Sterols / Q000032:analysis*; D014675:Vegetables
chemical_list: D013261:Sterols