[Use of modified acid elution technique in laboratory diagnosis of autoimmune hemolytic anemia].
Three modifications of acid elution method were used for the diagnosis of autoimmune haemolytic anaemia. These procedures were adapted to obtain more potent eluates and to prepare intact antibody-free red blood cells for phenotyping and for autologous absorption of warm-reactive autoantibodies. From the results presented in this paper we conclude that the modifications of the acid elution method are better than the up to now used procedure with a mixture of proteolytic enzyme and a thiol reagent. Moreover, these procedures are quick and easy to perform as well as more efficient. The obtained colourless eluates can be investigated in a gel-lest.
Fabijańska-Mitek
J
J
Zakładu Serologii Instytutu Hematologii i Transfuzjologii w Warszawie.
Kuśnierz-Alejska
G
G
D000328:Adult; D000744:Anemia, Hemolytic, Autoimmune / Q000175:diagnosis*; D001323:Autoantibodies / Q000097:blood; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D012698:Serologic Tests / Q000379:methods*
D001323:Autoantibodies
Zastosowanie zmodyfikowanej techniki kwaśnej elucji przeciwciał w diagnostyce laboratoryjnej niedokrwistości autoimmunohemolitycznej.