title: [Study of the interaction of DNA with copper ions using IR- and Raman spectroscopy].
abstract: DNA with Cu2+ ions in solution and films is studied by IR and Raman spectroscopy at different relative humidities (R.H. = 51 divided by 98%). DNA complexed with Cu2+ ions is shown to transit to the double-helix conformation, passing A-form, the water content per nucleotide (n) being essentially larger: 12 for Cu2+/P = 0.4 (n = 8 for DNA without ions). Cu2+ decrease the biopolymer hydration at R.H. 50-76% interactions with Cu2+ are stated to depend on the hydration degree of macromolecules. The analysis of the absorption band shifts of Raman spectra evidences the changes in the base torsion angle around the glycoside bond to the values characteristic of the syn-conformation and the interaction both with phosphates and nitrogen bases.
authors.forename: S V
authors.initials: SV
authors.lastname: Kornilova
authors.forename: L E
authors.initials: LE
authors.lastname: Kapinos
authors.forename: A
authors.initials: A
authors.lastname: Tomkova
authors.forename: P
authors.initials: P
authors.lastname: Mishkovskiĭ
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Blagoĭ
authors.forename: T V
authors.initials: TV
authors.lastname: Bol'bukh
mesh_terms: D002413:Cations, Divalent; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006813:Humidity; D009690:Nucleic Acid Conformation; D013055:Spectrophotometry, Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D002413:Cations, Divalent; D003300:Copper; D004247:DNA
vernacular_title: Izuchenie metodami IK- i Ramanovskoĭ spektroskopii vzaimodeĭstviia DNK s ionami medi.