SCM2, a tryptophan permease in Saccharomyces cerevisiae, is important for cell growth.
SCM2, a novel gene encoding a yeast tryptophan permease, was cloned as a high-copy-number suppressor of cse2-1. The cse2-1 mutation causes cold sensitivity, temperature sensitivity and chromosome missegregation. However, only the cold-sensitive phenotype of cse2-1 cells is suppressed by SCM2 at high copy. SCM2 is located on the left arm of yeast chromosome XV, adjacent to SUP3 and encodes a 65 kDa protein that is highly homologous to known amino acid permeases. Four out of five disrupted scm2 alleles (scm2 delta 1-delta 4) cause slow growth, whereas one disrupted allele (scm2 delta 5) is lethal. Cells with both the scm2 delta 1 and trp1-delta 101 mutations exhibit a synthetic cold-sensitive phenotype and grow much more slowly at the permissive temperature than cells with a single scm2 delta 1 or trp1-delta 101 mutation. A region of the predicted SCM2 protein is identical to the partial sequence recently reported for the yeast tryptophan permease TAP2, indicating that SCM2 and TAP2 probably encode the same protein.
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Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003-4505.
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Fitzgerald-Hayes
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D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems; D001483:Base Sequence; D001692:Biological Transport; D002455:Cell Division / Q000235:genetics; D002874:Chromosome Mapping; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D016153:Genes, Suppressor / Q000235:genetics*; D056892:Mediator Complex; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014364:Tryptophan / Q000378:metabolism
D026905:Amino Acid Transport Systems; C082275:CSE2 protein, S cerevisiae; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D056892:Mediator Complex; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C429825:TAT2 protein, S cerevisiae; D014157:Transcription Factors; C035131:TnaB protein, E coli; D014364:Tryptophan