Transforming activity of selected polycyclic aromatic hydrocarbons and their nitro-derivatives in BALB/3T3 A31-1-1 cells.
The transforming activities of four polycyclic aromatic hydrocarbons and six of their nitro-derivatives were studied using BALB/3T3 clone A31-1-1 cells in the absence of exogenous metabolic activation. Each compound was assayed two to four times to its maximal level of solubility. A transformation response was induced by 1-nitropyrene, 2-nitropyrene, 4-nitropyrene and benzo[a]pyrene in the BALB/3T3 mouse embryo cells. Pyrene and 7-nitrobenz[a]anthracene produced questionable responses, and benz[a]anthracene, chrysene, 6-nitrobenzo[a]pyrene and 6-nitrochrysene produced negative responses. The capacity of the assay system to indicate tumorigenicity of the test compounds is discussed.
Sheu
C W
CW
Genetic Toxicology Branch, Food and Drug Administration, Washington, DC 20204.
Dobras
S N
SN
Rodriguez
I
I
Lee
J K
JK
Fu
P P
PP
D016475:3T3 Cells / Q000187:drug effects*; D000818:Animals; D001551:Benz(a)Anthracenes / Q000494:pharmacology; D001564:Benzo(a)pyrene / Q000494:pharmacology; D001580:Benzopyrenes / Q000494:pharmacology; D015197:Carcinogenicity Tests / Q000379:methods*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002911:Chrysenes / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011083:Polycyclic Compounds / Q000494:pharmacology*; D011721:Pyrenes / Q000494:pharmacology
D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D002911:Chrysenes; D011083:Polycyclic Compounds; D011721:Pyrenes; C031180:chrysene; C038954:7-nitrobenzanthracene; D001564:Benzo(a)pyrene; C034699:6-nitrobenzo(a)pyrene; C049029:2-nitropyrene; C035986:6-nitrochrysene; C030984:pyrene; C030935:benz(a)anthracene; C032668:1-nitropyrene; C062494:4-nitropyrene