title: [Construction of the genomic library for cyanobacteria Synechocystis sp. PCC 6803 using a cosmid].
abstract: The gene library of the phototrophic cyanobacterium Synechocystis PCC6803 was constructed in the cosmid vector Lorist6. About -3 x 10(3) recombinant clones were obtained, of which 760 were selected for further work. Each clone was kept in an individual well of a microtiter plate. The clone grids obtained were hybridized with two DNA probes, which contained the ndhE and dfr genes of Synechocystis PCC6803. Two groups of probe-overlapping cosmids (11 and 8 clones respectively), containing insertions averaging of 39 kb, were isolated. The capacity of the gene library is nine equivalents of the Synechocystis PCC6803 genome and its representativity exceeds 99%.
authors.forename: V I
authors.initials: VI
authors.lastname: Shestopalov
authors.forename: O O
authors.initials: OO
authors.lastname: Nashchokina
authors.forename: S V
authors.initials: SV
authors.lastname: Shestakov
authors.forename: N K
authors.initials: NK
authors.lastname: Iankovskiĭ
mesh_terms: D003001:Cloning, Molecular; D003360:Cosmids; D000458:Cyanobacteria / Q000235:genetics*; D004269:DNA, Bacterial; D016680:Genome, Bacterial
chemical_list: D004269:DNA, Bacterial
vernacular_title: Konstruirovanie kosmidnoĭ biblioteki genov tsianobakterii Synechocystis sp. PCC 6803.