[The use of the physicochemical laws governing the sorption properties of antigens (antibodies) in relation to the plastic solid phase for enhancing the sensitivity of immunoenzyme analysis test systems in the diagnosis of infections].
Some practical aspects of the theory of adsorption of bioligands on plastic surfaces have been considered and discussed. Modifications of experimental and actual antigens (antibodies) have been theoretically and practically realized on the concept of "solubility parameters" by means of pin-point hydrophobization. The use of this method has been shown to increase the sensitivity of individual ELISA systems based on rabbit and mouse immunoglobulins 1.5- to 2-fold; the sensitivity of the assay system for the evaluation of HBsAg increases 8- to 10-fold. Theoretical calculations making it possible to map out the ways to further increase the sensitivity of different ELISA systems are given.
Meshandin
A G
AG
Dargeeva
T A
TA
Vaneeva
L I
LI
D000327:Adsorption; D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D000941:Antigens / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003141:Communicable Diseases / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D051379:Mice; D011137:Polystyrenes; D011817:Rabbits; D012680:Sensitivity and Specificity; D012995:Solubility
D000906:Antibodies; D000941:Antigens; D011137:Polystyrenes
Ispol'zovanie fiziko-khimicheskikh zakonomernosteĭ, upravliaiushchikh sorbtsionnymi svoĭstvami antigenov (antitel) po otnosheniiu k plastikovoĭ tverdoĭ faze, dlia uvelicheniia chuvstvitel'nosti immunofermentnogo analiza test-sistem pri diagnostike nekotorykh infektsiĭ.