title: Contribution of central and peripheral adrenergic stimulation to IL-1 alpha-mediated glucoregulation.
abstract: The present study determined the contribution of central adrenoceptors and the peripheral sympathetic nervous system in regulating the hormonal and glucose metabolic response to intracerebroventricular injection of interleukin (IL)-1 alpha. After an overnight fast, hepatic glucose production (HGP) and peripheral glucose uptake (GU) were assessed in catheterized conscious unrestrained rats using [3-3H]glucose. Intracerebroventricular injection of IL-1 alpha (100 ng) produced a hyperglycemia that resulted from an early increase in HGP (108%) that exceeded a smaller elevation (82%) in GU. Intracerebroventricular injection of the alpha- and beta-adrenergic antagonists phentolamine and propranolol before IL-1 alpha blunted the glucose metabolic response 30-50%. This attenuated response was associated with normalization of the IL-1 alpha-induced hyperglucagonemia and hyperinsulinemia and a 50-60% reduction in the incremental increase in plasma catecholamines. In contrast to central administration, systemic infusion of adrenergic blockers completely prevented the IL-1 alpha-induced increases in plasma glucose, as well as HGP and GU. In these rats, the elevated plasma levels of insulin, glucagon, and corticosterone produced by intracerebroventricular injection of IL-1 alpha were still present. The results indicate that 1) the enhanced whole body glucose metabolism seen after central administration of IL-1 alpha is mediated by increased sympathoadrenal activity and 2) the IL-1 alpha-induced increase in pancreatic insulin and glucagon secretion as well as part of the peripheral catecholamine release is mediated by central adrenoreceptors.
authors.affiliation: Department of Surgery, State University of New York at Stony Brook 11794-8191.
authors.forename: F
authors.initials: F
authors.lastname: Petit
authors.forename: A
authors.initials: A
authors.lastname: Jarrous
authors.forename: R D
authors.initials: RD
authors.lastname: Dickinson
authors.forename: P E
authors.initials: PE
authors.lastname: Molina
authors.forename: N N
authors.initials: NN
authors.lastname: Abumrad
authors.forename: C H
authors.initials: CH
authors.lastname: Lang
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D001921:Brain / Q000502:physiology*; D002395:Catecholamines / Q000097:blood; D005947:Glucose / Q000378:metabolism*; D006728:Hormones / Q000097:blood; D007275:Injections, Intravenous; D007276:Injections, Intraventricular; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D009994:Osmolar Concentration; D051381:Rats; D017207:Rats, Sprague-Dawley; D013566:Sympathomimetics / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D002395:Catecholamines; D006728:Hormones; D007375:Interleukin-1; D013566:Sympathomimetics; D005947:Glucose