title: A simple method for N-15 labelling of exocyclic amino groups in synthetic oligodeoxynucleotides.
abstract: The use of the ammonia deprotection step to introduce (15)N labels at specific exocyclic amino positions of adenine, cytosine, guanine or 2-aminopurine of oligodeoxynucleotides is described.
authors.affiliation: Department of Molecular Genetics, CID-CSIC, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Acedo
authors.forename: C
authors.initials: C
authors.lastname: Fàbrega
authors.forename: A
authors.initials: A
authors.lastname: Aviño
authors.forename: M
authors.initials: M
authors.lastname: Goodman
authors.forename: P
authors.initials: P
authors.lastname: Fagan
authors.forename: D
authors.initials: D
authors.lastname: Wemmer
authors.forename: R
authors.initials: R
authors.lastname: Eritja
mesh_terms: D015075:2-Aminopurine; D000225:Adenine; D000641:Ammonia; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003596:Cytosine; D006147:Guanine; D007553:Isotope Labeling / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis
chemical_list: D009587:Nitrogen Isotopes; D009838:Oligodeoxyribonucleotides; D015075:2-Aminopurine; D006147:Guanine; D000641:Ammonia; D003596:Cytosine; D000225:Adenine