title: cDNA cloning of human kidney pyruvate carboxylase.
abstract: cDNA clones covering a total of 4017 bp were isolated corresponding to the full length of the message for human pyruvate carboxylase. The coding sequence consisted of a stretch of 3534 bp flanked by a 5' untranslated sequence of 82 bp and a 3' untranslated region of 389 bp excluding the poly-A tail. The translated amino acid sequence of 1178 residues contained consensus sequences for the covalent attachment for biotin, the binding of ATP and the binding of the substrate, pyruvate.
authors.affiliation: Department of Biochemistry, University of Toronto, Ontario, Canada.
authors.forename: N
authors.initials: N
authors.lastname: MacKay
authors.forename: B
authors.initials: B
authors.lastname: Rigat
authors.forename: C
authors.initials: C
authors.lastname: Douglas
authors.forename: H S
authors.initials: HS
authors.lastname: Chen
authors.forename: B H
authors.initials: BH
authors.lastname: Robinson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011766:Pyruvate Carboxylase / Q000737:chemistry / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D011766:Pyruvate Carboxylase