Lignans and flavonoids inhibit aromatase enzyme in human preadipocytes.
Lignans and flavonoids are naturally-occurring diphenolic compounds found in high concentrations in whole grains, legumes, fruits and vegetables. Seven lignans and six flavonoids were evaluated for their abilities to inhibit aromatase enzyme activity in a human preadipose cell culture system. The lignan, enterolactone (Enl) and its theoretical precursors, 3'-demethoxy-3O-demethylmatairesinol (DMDM) and didemethoxymatairesinol (DDMM) decreased aromatase enzyme activity, with Ki values of 14.4, 5.0 and 7.3 microM, respectively. The flavonoids, coumestrol, luteolin and kaempferol also decreased aromatase enzyme activity, with Ki values of 1.3, 4.8 and 27.2 microM, respectively. Aminoglutethimide, a pharmaceutical aromatase inhibitor, showed a Ki value of 0.5 microM. Kinetic studies showed the inhibition by all compounds to be competitive. Smaller decreases in aromatase activity were observed with the lignan, enterodiol (End) and its theoretical precursors, O-demethylsecoisolariciresinol (ODSI), demethoxysecoisolariciresinol (DMSI) and didemethylsecoisolariciresinol (DDSI). The flavonoids, O-demethylangolensin (O-Dma), fisetin and morin showed no inhibitory effects. The inhibition of human preadipocyte aromatase activity by lignans and flavonoids suggests a mechanism by which consumption of lignan- and flavonoid-rich plant foods may contribute to reduction of estrogen-dependent disease, such as breast cancer.
Wang
C
C
Department of Food Science and Nutrition, University of Minnesota, St Paul 55108.
Mäkelä
T
T
Hase
T
T
Adlercreutz
H
H
Kurzer
M S
MS
D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000368:Aged; D047072:Aromatase Inhibitors; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D017705:Lignans / Q000494:pharmacology*
D047072:Aromatase Inhibitors; D005419:Flavonoids; D017705:Lignans