UV-transparent, replaceable agarose gels for molecular-sieve (capillary) electrophoresis of proteins and nucleic acids.
Gels of methoxylated agarose (gelling point 25.6 degrees C) and other low-melting agarose derivatives compare favorably with cross-linked polyacrylamide gels for capillary and slab molecular-sieve electrophoresis of proteins and DNA. These agarose gels can be pressed out of the capillary following a run and replaced by an agarose solution with a temperature of 35-40 degrees C. Gelation occurs upon lowering temperature and the same capillary can thus be reused for another analysis with a fresh gel. The methoxylated, non UV-absorbing agarose gels are, accordingly, replaceable, which makes them very attractive for series analyses with modern, automated capillary electrophoresis apparatus. The high resolution of these agarose gels is demonstrated with a separation of an albumin sample into monomers, dimers, trimers, tetramers, pentamers, hexamers, heptamers, and of DNA fragments.
Hjertén
S
S
Department of Biochemistry, Uppsala University, Sweden.
Srichaiyo
T
T
Palm
A
A
D001322:Autoanalysis; D002197:Capillary Action; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011506:Proteins / Q000032:analysis*; D012685:Sepharose / Q000737:chemistry; D012709:Serum Albumin / Q000302:isolation & purification; D013696:Temperature
D011506:Proteins; D012709:Serum Albumin; D004247:DNA; D012685:Sepharose