Isolation of prolylendopeptidase-inhibiting peptides from bovine brain.
The peptides inhibiting prolylendopeptidase were screened and isolated from bovine brain by monitoring the inhibition of prolylendopeptidase produced by Flavobacterium meningosepticum. The amino acid sequence of the peptide having the highest inhibitory activity was determined as Met-Pro-Pro-Pro-Leu-Pro-Ala-Arg-Val-Asp-Phe-Ser-Leu-Ala-Gly-Ala-Leu-Asn. This peptide showed the IC50 and Ki values of 38.4 and 8.6 microM, respectively, for prolylendopeptidase isolated from bovine brain.
Ohmori
T
T
Department of Health and Dietary Science, Nippon Meat Packers, Inc., Ibaraki, Japan.
Nakagami
T
T
Tanaka
H
H
Maruyama
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005417:Flavobacterium / Q000201:enzymology; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D010455:Peptides; D015842:Serine Proteinase Inhibitors; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases