Delineation of molecular species of a family of diacyltrehaloses from Mycobacterium fortuitum by mass spectrometry.
A family of 2,3-di-O-acyl trehaloses (DAT), previously identified in Mycobacterium fortuitum, was studied by fast-atom bombardment mass spectrometry to establish the combinations of fatty acyl substituents and, hence, to delineate the molecular species there comprised. The mass spectra indicated the possible existence of 41 molecular species, with a total of 35-40 carbon atoms and 0-4 double bonds in the lipid moiety. The principal components were situated a m/z (M+ +23) 919 (formulated as 2-methyloctadec-2-enoyl,2-methyloctadecadienoyl trehalose and as 2-methylhexadec-2-enoyl,2-methyleicosadienoyl trehalose) and m/z (M+ +23) 921 (formulated as di-2-methyloctadec-2-enoyl trehalose and as 2-methylhexadec-2-enoyl,2-methyleicos-2-enoyl trehalose). The data obtained revealed that DAT was composed of three types of combinations of fatty acyl groups; (i) linear plus linear; (ii) linear plus 2-methyl branched; and (iii) 2-methyl branched plus 2-methyl branched.
Ariza
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MA
Departamento de Genética y Microbiología, Universidad de Murcia, Facultad de Medicina, Campus Universitario de Espinardo, Spain.
Valero-Guillén
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PL
D013058:Mass Spectrometry; D009161:Mycobacterium / Q000737:chemistry*; D014199:Trehalose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification
D014199:Trehalose