Human neuroblastoma growth inhibitory factor (h-NGIF), derived from human astrocytoma conditioned medium, has neurotrophic properties.
Investigations on the general characteristics of human astrocytoma cell line NAC-1 revealed neuroblastoma growth inhibitory activity in conditioned medium. Neuroblastoma growth inhibitory factor (NGIF) was partially purified by Econo Q, Econo CM, and Superose 12 column chromatography. The protein is weakly basic with an estimated M(r) of 120,000, possibly having an M(r) 60,000 dimeric structure. NGIF inhibits the growth of human neuroblastoma cell lines but has no effect on morphology nor does it produce any change in the growth of human glioblastoma cell lines. Interestingly, NGIF appears to promote survival and neurite outgrowth of embryonal rat cortical neurons. These neurotrophic properties suggest a role for NGIF in the development of the nervous system.
Eksioglu
Y Z
YZ
Department of Bioregulation Research, Nagoya City University Medical School, Japan.
Iida
J
J
Asai
K
K
Ueki
T
T
Nakanishi
K
K
Isobe
I
I
Yamagata
K
K
Kato
T
T
D000818:Animals; D001254:Astrocytoma / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects; D017077:Culture Media, Conditioned; D010450:Endopeptidases; D006801:Humans; D006868:Hydrolysis; D051379:Mice; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009423:Nervous System Neoplasms / Q000378:metabolism*; D009457:Neuroglia / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D009419:Nerve Tissue Proteins; D010450:Endopeptidases