Sulfide dehydrogenase is identical with the SoxB protein of the thiosulfate-oxidizing enzyme system of Paracoccus denitrificans GB17.
Thiosulfate induced cells of Paracoccus denitrificans GB17 oxidize thiosulfate and sulfide to sulfate. A mutant carrying a Tn5-mob insertion in the soxB gene is unable to oxidize thiosulfate or sulfide suggesting a linkage of both activities. To test this assumption we have separated the components of the thiosulfate-oxidizing enzyme system of the wild-type by ion exchange chromatography. The SoxB protein coeluted with a highly active sulfide dehydrogenase. Analysis by polyacrylamide gel electrophoresis revealed one major protein of M(r) 32k. Thus, the SoxB protein appeared to be identical with sulfide dehydrogenase.
Schneider
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A
Lehrstuhl für Technische Mikrobiologie, Fachbereich Chemietechnik, Universität Dortmund, Germany.
Friedrich
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C
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D003574:Cytochrome c Group / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification*; D010231:Paracoccus denitrificans / Q000201:enzymology*; D013440:Sulfides / Q000378:metabolism
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D013440:Sulfides; D010088:Oxidoreductases; C064500:flavocytochrome c sulfide dehydrogenase