title: Differential lectin binding of Onchocerca lienalis and Onchocerca ochengi infective larvae following their development in Simulium ornatum s.l.
abstract: Lectins have been used to investigate species specific differences in carbohydrate moieties on the surface of the infective larvae of two Onchocerca species following their development in Simulium ornatum. Of the seven FITC-labelled lectins used in this study only two, Arachis hypogea (PNA) and Helix pomatia (HPA), bound to the surface of O. lienalis, whereas no lectin binding could be detected on the surface of O. ochengi infective larvae. This indicates that in principal lectins might be a potential tool for the differentiation of more closely related Onchocerca species.
authors.affiliation: Department of Biological Sciences, University of Keele, Staffordshire, UK.
authors.forename: H E
authors.initials: HE
authors.lastname: Hagen
authors.forename: J
authors.initials: J
authors.lastname: Grunewald
authors.forename: P J
authors.initials: PJ
authors.lastname: Ham
mesh_terms: D000818:Animals; D001665:Binding Sites; D002240:Carbohydrate Sequence; D002417:Cattle; D005260:Female; D007814:Larva / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008842:Microfilariae / Q000378:metabolism; D008969:Molecular Sequence Data; D009854:Onchocerca / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D019887:Peanut Agglutinin; D012843:Simuliidae / Q000469:parasitology; D013045:Species Specificity
chemical_list: C038095:Helix lectin; D037102:Lectins; D019887:Peanut Agglutinin