Improved thin layer chromatographic resolution of PTH amino acids with some new solvent systems.
Three new solvent systems, pyridine: benzene (2.5:20, v/v), methanol:carbon tetrachloride (1:20, v/v) and acetone:dichloromethane (0.3:8, v/v), for the resolution of a mixture of 18 PTH amino acids have been reported. Using these solvent systems, various combinations of PTH amino acids which had previously posed resolution problems have been resolved and identified.
Bhushan
R
R
Department of Chemistry, University of Roorkee, India.
Mahesh
V K
VK
Varma
A
A
D000096:Acetone; D000596:Amino Acids / Q000032:analysis / Q000302:isolation & purification*; D001554:Benzene; D002251:Carbon Tetrachloride; D002855:Chromatography, Thin Layer / Q000379:methods* / Q000706:statistics & numerical data; D000432:Methanol; D008752:Methylene Chloride; D010669:Phenylthiohydantoin; D011725:Pyridines; D012997:Solvents
D000596:Amino Acids; D011725:Pyridines; D012997:Solvents; D000096:Acetone; D010669:Phenylthiohydantoin; D008752:Methylene Chloride; D002251:Carbon Tetrachloride; D001554:Benzene; C023666:pyridine; D000432:Methanol