title: Novel benzisoxazole derivatives as potent and selective inhibitors of acetylcholinesterase.
abstract: A series of N-benzylpiperidine benzisoxazoles has been developed as potent and selective inhibitors of the enzyme acetylcholinesterase (AChE). The benzisoxazole heterocycle was found to be an appropriate bioisosteric replacement for the benzoyl functionality present in the N-benzylpiperidine class of inhibitors. The title compounds were synthesized by alkylating 3-methyl-1,2-benzisoxazoles with an iodo piperidine derivatives as the key step. Benzisoxazoles 1b-j,o displayed potent inhibition of AChE in vitro with IC50's = 0.8-14 nM. Particularly interesting were N-acetyl and morpholino derivatives 1g (IC50 = 3 nM) and 1j (IC50 = 0.8 nM), respectively, which displayed outstanding selectivity for acetyl-over butyrylcholinesterase, in excess of 3 orders of magnitude. N-Acetyl 1g also displayed a favorable profile in vivo. This analog showed a dose-dependent elevation of total acetylcholine in mouse forebrain after oral administration with an ED50 = 2.4 mg/kg. In addition, 1g was able to reverse amnesia in a mouse passive avoidance model at doses of 3.2 and 5.6 mg/kg with an average reversal of 89.7%. Molecular dynamics simulations were used to study the possible binding modes of N-benzylpiperidine benzisoxazoles to AChE from Torpedo californica. Key structural insights were obtained regarding the potency of this class of inhibitors. Specifically, Asp-72, Trp-84, Trp-279, Phe-288, and Phe-330 are implicated in the binding of these inhibitors. The N-benzylpiperidine benzisoxazoles may be suitable compounds for the palliative treatment of Alzheimer's Disease.
authors.affiliation: Department of Medicinal Chemistry, Central Research Division, Pfizer Inc., Groton, Connecticut 06340.
authors.forename: A
authors.initials: A
authors.lastname: Villalobos
authors.forename: J F
authors.initials: JF
authors.lastname: Blake
authors.forename: C K
authors.initials: CK
authors.lastname: Biggers
authors.forename: T W
authors.initials: TW
authors.lastname: Butler
authors.forename: D S
authors.initials: DS
authors.lastname: Chapin
authors.forename: Y L
authors.initials: YL
authors.lastname: Chen
authors.forename: J L
authors.initials: JL
authors.lastname: Ives
authors.forename: S B
authors.initials: SB
authors.lastname: Jones
authors.forename: D R
authors.initials: DR
authors.lastname: Liston
authors.forename: A A
authors.initials: AA
authors.lastname: Nagel
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects*; D001665:Binding Sites; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D003196:Computer Graphics; D007555:Isoxazoles / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D009025:Morpholines / Q000138:chemical synthesis / Q000494:pharmacology; D010880:Piperidines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D016548:Prosencephalon / Q000187:drug effects / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D002800:Cholinesterase Inhibitors; D007555:Isoxazoles; D009025:Morpholines; D010880:Piperidines; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D000109:Acetylcholine