Effect of pH and temperature on the stability of UV-induced repairable pyrimidine hydrates in DNA.
UV irradiation of cytosine yields 6-hydroxy-5,6-dihydrocytosine (cytosine hydrate) whether the cytosine is in solution as base, nucleoside, or nucleotide or on the DNA backbone. Cytosine hydrate decomposes by elimination of water, yielding cytosine, or by irreversible deamination, yielding uracil hydrate, which, in turn, decomposes by dehydration yielding uracil. To determine how pH and temperature affect these decomposition reactions, alternating poly(dG-[3H]dC) copolymer was irradiated at 254 nm and incubated under different conditions of pH and temperature. The cytosine hydrate and uracil hydrate content of the DNA was determined by the use of Escherichia coli endonuclease III, which releases pyrimidine hydrates from DNA by virtue of its DNA glycosylase activity. Uracil content was determined by using uracil-DNA glycosylase. The rate of decomposition of cytosine hydrate to cytosine was determined at 4 temperatures at pH 3.1, 5.4, and 7.4. The Ea was determined from the rates by using the Arrhenius equation and proved to be the same at pH 5.4 and 7.4, although the decomposition rate at pH 5.4 was faster at all temperatures. At pH 3.1, the Ea was reduced. These results suggest that the dehydration reaction is affected by two discrete protonations, most probably of the N-3 and the OH group of C-6 of cytosine hydrate. The deamination of cytosine hydrate to uracil hydrate was maximal at pH 3.1 at all temperatures. The doubly protonated cytosine hydrate probably is the common intermediate for both competing decomposition reactions, explaining why cytosine hydrate is prone to deamination at acid pH.(ABSTRACT TRUNCATED AT 250 WORDS)
O'Donnell
R E
RE
Department of Pathology, Rita and Stanley Kaplan Cancer Center, New York University Medical Center, New York 10016.
Boorstein
R J
RJ
Cunningham
R P
RP
Teebor
G W
GW
D003596:Cytosine / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D004355:Drug Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism*; D013696:Temperature; D013816:Thermodynamics; D014466:Ultraviolet Rays; D014498:Uracil / Q000378:metabolism
D011089:Polydeoxyribonucleotides; D011743:Pyrimidines; C019530:poly(dC-dG); D014498:Uracil; D003596:Cytosine; D004247:DNA; C030986:pyrimidine