title: Changes in the cell wall glycoprotein composition of Candida albicans associated to the inhibition of germ tube formation by EDTA.
abstract: Hyphal development in Candida albicans was blocked by EDTA. This effect was not due to a general growth inhibition since the chelator did not affect protein and DNA synthesis. Recovery of mycelial growth was observed when EDTA-grown cells were incubated at 37 degrees C in EDTA-free medium. High-molecular-weight mannoproteins (HMWM) that are mycelium-specific wall components, and particularly a 260-kDa species (HMWM-260), were absent in the wall of cells grown under germination conditions in the presence of EDTA. Synthesis of the HMWM-260 species was not inhibited but its incorporation (secretion) into the wall structure seemed to be blocked in EDTA-treated cells.
authors.affiliation: Sección Departamental de Microbiología, Facultad de Farmacia, Universitat de Valencia, Burjassot, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Gil
authors.forename: M
authors.initials: M
authors.lastname: Casanova
authors.forename: J P
authors.initials: JP
authors.lastname: Martínez
mesh_terms: D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology*; D005656:Fungal Proteins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D011523:Protoplasts
chemical_list: D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C025363:mannoproteins; D004492:Edetic Acid