title: Rapid characterisation of Candida albicans by pyrolysis mass spectrometry.
abstract: Clinical isolates (41) of Candida spp. from three possible outbreaks of nosocomially-acquired infection were compared by pyrolysis mass spectrometry (PMS) and by a combined morphotyping and resistotyping (M-R typing) method. Both systems characterised all the isolates and distinguished one isolate of C. tropicalis and another of C. glabrata from the 39 isolates of C. albicans. Results from both systems suggested that cross-infection with a single strain contributed to two of the outbreaks. In several instances, more than one strain of C. albicans was found amongst multiple isolates from the same patient. PMS is a simple, rapid and objective technique capable of characterising C. albicans isolates; discrimination was similar to M-R typing.
authors.affiliation: Laboratory of Hospital Infection, Central Public Health Laboratory, London.
authors.forename: G C
authors.initials: GC
authors.lastname: White
authors.forename: P R
authors.initials: PR
authors.lastname: Sisson
authors.forename: R
authors.initials: R
authors.lastname: Freeman
authors.forename: B D
authors.initials: BD
authors.lastname: Cookson
mesh_terms: D002176:Candida albicans / Q000145:classification* / Q000187:drug effects; D002177:Candidiasis / Q000453:epidemiology / Q000382:microbiology*; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D004196:Disease Outbreaks; D004352:Drug Resistance, Microbial; D006801:Humans; D013058:Mass Spectrometry; D015203:Reproducibility of Results