Rat olfactory neurons can utilize the endogenous lectin, L-14, in a novel adhesion mechanism.
L-14 is a divalent, lactosamine-binding lectin expressed in many vertebrate tissues. In the rat nervous system, L-14 expression has been observed previously in restricted neuronal subsets within the dorsal root ganglia and spinal cord. In this study we report that L-14 is expressed by nonneuronal cells in the rat olfactory nerve. We demonstrate that L-14 binds and co-localizes with two ligands in the rat olfactory system: a beta-lactosamine-containing glycolipid, and a putative member of the laminin family. The former is expressed on the surfaces of nascent olfactory axons originating from neuron cell bodies in the olfactory epithelium. The latter is present in the extracellular matrix of the axonal path leading to synaptic targets in the olfactory bulb. In vitro, we find that recombinant L-14 promotes primary olfactory neuron adhesion to two laminin family members, and promotes intercellular adhesion. Both activities are dose-dependent, and are independent of integrin-mediated mechanisms. We have thus found that L-14 can serve two distinct adhesive functions in vitro, and propose that L-14 in vivo can promote olfactory axon fasciculation by crosslinking adjacent axons and promote axonal adhesion to the extracellular matrix.
Mahanthappa
N K
NK
E. K. Shriver Center for Mental Retardation, Waltham, MA 02254.
Cooper
D N
DN
Barondes
S H
SH
Schwarting
G A
GA
D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D005109:Extracellular Matrix / Q000378:metabolism; D005697:Galactosides / Q000378:metabolism*; D037483:Galectin 1; D006388:Hemagglutinins / Q000378:metabolism* / Q000494:pharmacology; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009832:Olfactory Nerve / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
D017931:DNA Primers; D005697:Galactosides; D037483:Galectin 1; D006388:Hemagglutinins; D007797:Laminin