A creatine transporter cDNA from Torpedo illustrates structure/function relationships in the GABA/noradrenaline transporter family.
A family of homologous, Na+/Cl- dependent plasma membrane transporters catalyze the uptake of a number of neurotransmitters and structurally related compounds into cells. Here, we report the cDNA cloning, sequencing and functional characterization of a non-mammalian member of this transporter family. A creatine transporter from the electric ray, Torpedo marmorata, displays 64% amino acid identity with its rabbit counterpart and has a similar substrate affinity and specificity. Sequence similarity generally is lowest in those regions where also the sequences of other members of the family, transporting different substrates, diverge. Only a few amino acids are better conserved between the two creatine transporters than with the other family members and are candidates for a role in conferring substrate specificity.
Guimbal
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Institut für Physiologische Chemie, Medizinische Fakultät Ruhr-Universität Bochum, Germany.
Kilimann
M W
MW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D003401:Creatine / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D050485:GABA Plasma Membrane Transport Proteins; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009638:Norepinephrine / Q000378:metabolism; D050484:Norepinephrine Plasma Membrane Transport Proteins; D027361:Organic Anion Transporters; D016415:Sequence Alignment; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D027981:Symporters; D014101:Torpedo / Q000235:genetics*; D014162:Transfection; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D002352:Carrier Proteins; D018076:DNA, Complementary; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D050484:Norepinephrine Plasma Membrane Transport Proteins; D027361:Organic Anion Transporters; C493227:SLC6A2 protein, human; D027981:Symporters; C080679:creatine transporter; D005680:gamma-Aminobutyric Acid; D003401:Creatine; D009638:Norepinephrine