[Experimental studies on peripheral-type benzodiazepine receptors in brain in rats with hepatic encephalopathy].
By means of radioligand binding assay, peripheral-type benzodiazepine receptors (PBR) in the telencephalon, diencephalon, brain stem and cerebellum, were investigated in a rat model with hepatic encephalopathy (HE). Binding to PBR was studied using [3H]PK11195 as radioligand. It was demonstrated that only densities (Bmax) of [3H]PK11195 binding to crude synaptosomal membranes in the telencephalon, diencephalon of HE rats were obviously increased as compared with those of controls (P < 0.001 and P < 0.05 respectively). Increased affinities (Kd) were only observed in brain stem (P < 0.05). The results showed that the alterations of PBR in different regions of the brain play a significant role in the pathogenesis of HE.
Zhang
Z M
ZM
Tongji Hospital, Tongji Medical University, Wuhan.
Qiu
F Z
FZ
Chen
X P
XP
D000818:Animals; D001923:Brain Chemistry; D006501:Hepatic Encephalopathy / Q000378:metabolism*; D017093:Liver Failure / Q000150:complications; D008297:Male; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000032:analysis*
D011963:Receptors, GABA-A