title: Apoptosis is induced by 1-methyl-4-phenylpyridinium ion (MPP+) in ventral mesencephalic-striatal co-culture in rat.
abstract: Apoptosis may be an important mechanism of cell death in some experimental cell death models as well as in human neurodegenerative disorders. We report evidence to indicate that 1-methyl-4-phenylpyridinium ion (MPP+) induces apoptotic cell death using ventral mesencephalic and striatal co-culture; Apoptosis was defined by characteristic changes in nuclear morphology as revealed by hematoxylin-staining and DNA breaks in situ, which were demonstrated histochemically by labeling 3'-OH ends with biotinylated deoxyuridine through the action of terminal deoxynucleotidyltransferase, and DNA fragmentation, which was shown by agarose-gel electrophoresis. Apoptosis may be an important mechanism of cell death in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism.
authors.affiliation: Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mochizuki
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Nishi
authors.forename: Y
authors.initials: Y
authors.lastname: Mizuno
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004247:DNA / Q000276:immunology / Q000378:metabolism; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000473:pathology*; D017072:Neostriatum / Q000473:pathology*; D009924:Organ Culture Techniques; D011726:Pyridinium Compounds / Q000494:pharmacology*; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011726:Pyridinium Compounds; C026560:1-(4-methoxyphenyl)pyridinium; D004247:DNA; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D004253:DNA Nucleotidylexotransferase