In vitro studies on the effect of thromboxane receptor blockade on platelet deposition on vascular surfaces.
The efficacy of platelet inhibition by a thromboxane receptor antagonist (Bay U3405) and acetylsalicylic acid was investigated in vitro using a new perfusion system. Rabbit 51Cr-labeled platelets were suspended in saline as perfusate. Vascular grafts, PTFE or Dacron (precoated with blood or uncoated) as well as native aorta were perfused in vitro. Three sets of grafts (vessels) were perfused simultaneously with either acetylsalicylic acid (10(-5) M), Bay U3405 (10(-6) M) treated or untreated (control) labeled platelets. Platelet deposition on the grafts (vessels) was measured in a gamma counter. Values were normalised to graft weight or vessel surface area respectively and to the platelet concentration of perfusate. The results of these experiments indicated that thromboxane receptor inhibition was superior to cyclooxygenase blockade in preventing platelet deposition on synthetic grafts and native aorta vessels in vitro. Whether this is valid in vivo has to be confirmed.
Xia
G
G
Department of Surgery, Malmö General Hospital, University of Lund, Sweden.
Lindberg
K
K
Risberg
B
B
D000818:Animals; D001011:Aorta / Q000187:drug effects; D001241:Aspirin / Q000494:pharmacology; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000187:drug effects*; D002227:Carbazoles / Q000494:pharmacology*; D066298:In Vitro Techniques; D016014:Linear Models; D010477:Perfusion; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D017482:Receptors, Thromboxane / Q000037:antagonists & inhibitors*; D013449:Sulfonamides / Q000494:pharmacology*
D002227:Carbazoles; D010975:Platelet Aggregation Inhibitors; D017482:Receptors, Thromboxane; D013449:Sulfonamides; C063119:ramatroban; D001241:Aspirin