1H magnetic resonance spectroscopy of primary human and murine cells of the myeloid lineage.
Murine and human cells of the myeloid lineage can exhibit proton spectra arising primarily from neutral lipid such as triglyceride, similar to other activated and transformed cell types. These spectral characteristics are independent of proliferation or proliferative ability and can be induced in vivo and in vitro in murine peritoneal macrophages by Listeria and interferon-gamma stimulation, respectively. In human peripheral monocytes, this spectrum can develop in the apparent absence of external activating stimuli such as interferon or endotoxin and also occurs in the absence of proliferation. Furthermore, 16-h culture of mixed peripheral blood cells causes concomitant and similar spectral changes in lymphocytes, which do not occur in pure lymphocyte cultures. The lipid spectra induced in mixed cultures develop to the same extent regardless of cell density or the ability to adhere or form aggregates and regardless of whether serum supplementation is of fetal bovine or adult human origin. Unlike peritoneal macrophages, the addition of interferon-gamma causes no additional spectral changes in adherent or nonadherent cultures. However, reduction of serum concentrations in culture medium causes a dose-dependent decrease in the acquired lipid signal. The observed spectral differences between human and murine myeloid populations could be species-dependent but could also be due to their differentiation status.
King
N J
NJ
Department of Pathology, University of Sydney, New South Wales, Australia.
Delikatny
E J
EJ
Holmes
K T
KT
D000818:Animals; D001790:Blood Physiological Phenomena; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods; D006801:Humans; D006859:Hydrogen; D007371:Interferon-gamma / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*
D008563:Membrane Lipids; D006859:Hydrogen; D007371:Interferon-gamma