Endogenous synthesis of N-methylnorsalsolinol in rat brain during in vivo microdialysis with epinine.
The in vivo metabolic pathway for the synthesis of N-methylnorsalsolinol, an analogue of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), was studied in the rat brain. N-Methyldopamine (epinine) was perfused at the striatum of the rat brain by in vivo microdialysis. N-Methylnorsalsolinol (NMNSAL) was identified in the brain dialysate after epinine perfusion using gas chromatography-selected-ion monitoring mass spectrometry (GC-SIM-MS). We demonstrated that NMNSAL could be synthesized from epinine with an aldehyde by the Piclet-Spengler condensation reaction in the rat brain.
Kajita
M
M
Department of Pediatrics, Nagoya University School of Medicine, Japan.
Niwa
T
T
Maruyama
W
W
Nakahara
D
D
Takeda
N
N
Yoshizumi
H
H
Tatematsu
A
A
Watanabe
K
K
Naoi
M
M
Nagatsu
T
T
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry; D003846:Deoxyepinephrine / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007546:Isoquinolines / Q000032:analysis / Q000138:chemical synthesis / Q000378:metabolism*; D017551:Microdialysis; D051381:Rats; D017208:Rats, Wistar; D044005:Tetrahydroisoquinolines
D000447:Aldehydes; D007546:Isoquinolines; D044005:Tetrahydroisoquinolines; C069194:2-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline; D003846:Deoxyepinephrine