Vascular endothelial growth factor induces the disorganization of actin stress fibers accompanied by protein tyrosine phosphorylation and morphological change in Balb/C3T3 cells.
The effects of vascular endothelial growth factor (VEGF) on cytoskeletal actin networks, cell morphology and tyrosine phosphorylation of cellular proteins were studied in Balb/c3T3 A31-1-1 cells. VEGF induced the disorganization of actin stress fibers accompanied by drastic morphological rounding. A binding assay of [125I]VEGF demonstrated high and low affinity receptors for VEGF in the cells. Moreover, VEGF induced tyrosine phosphorylation of at least five cellular proteins in a dose and a time dependent manner. A tyrosine kinase inhibitor, genistein, inhibited the VEGF-induced morphological rounding. These results indicate that VEGF exerts the alteration of cytoskeletal organization resulting in morphological changes through inducing receptor-mediated tyrosine phosphorylation of cellular proteins.
Enomoto
T
T
Department of Radiation Biophysics and Genetics, Kobe University School of Medicine, Japan.
Okamoto
T
T
Sato
J D
JD
D016475:3T3 Cells; D000199:Actins / Q000378:metabolism*; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001665:Binding Sites; D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D019833:Genistein; D007529:Isoflavones / Q000494:pharmacology; D008222:Lymphokines / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009036:Moths; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D014443:Tyrosine / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D000199:Actins; D016228:Endothelial Growth Factors; D007529:Isoflavones; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014443:Tyrosine; D019833:Genistein; D011505:Protein-Tyrosine Kinases