title: Immunohistochemical demonstration of chromogranin A, chromogranin B, and secretogranin II in extra-adrenal paragangliomas.
abstract: Twelve sympathetic and 14 parasympathetic extra-adrenal paragangliomas were investigated immunohistochemically with antibodies against chromogranin A, chromogranin B, and secretogranin II. In sympathetic paragangliomas chromogranin A was found in 12/12 and chromogranin B in 11/12 tumors in almost all chief cells (the remaining tumor was focally chromogranin B positive), whereas secretogranin II was immunolocalized in the majority of chief cells in 5/12, in a focal distribution in 3/12, and only in a few scattered tumor cells in 3/12 cases. One case showed no secretogranin II immunoreactivity. In parasympathetic paragangliomas both chromogranin B and secretogranin II immunoreactivity was demonstrated in the majority of chief cells of all 14 tumors investigated. Chromogranin A showed a strong immunostaining in 2/14 cases; in 12 tumors chromogranin A was found in only a few chief cells or was completely absent. It is concluded that sympathetic and parasympathetic paragangliomas show a divergent expression of chromogranins/secretogranins that apparently reflects the different histogenetic origins of these tumors.
authors.affiliation: Department of Pathology, University of Münster, Germany.
authors.forename: K W
authors.initials: KW
authors.lastname: Schmid
authors.forename: S
authors.initials: S
authors.lastname: Schröder
authors.forename: B
authors.initials: B
authors.lastname: Dockhorn-Dworniczak
authors.forename: R
authors.initials: R
authors.lastname: Kirchmair
authors.forename: M
authors.initials: M
authors.lastname: Tötsch
authors.forename: W
authors.initials: W
authors.lastname: Böcker
authors.forename: R
authors.initials: R
authors.lastname: Fischer-Colbrie
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D053379:Chromogranin A; D053378:Chromogranin B; D002864:Chromogranins / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000032:analysis; D009423:Nervous System Neoplasms / Q000737:chemistry* / Q000473:pathology; D010236:Paraganglioma, Extra-Adrenal / Q000737:chemistry* / Q000473:pathology; D010275:Parasympathetic Nervous System / Q000737:chemistry / Q000473:pathology; D011506:Proteins / Q000032:analysis*; D013564:Sympathetic Nervous System / Q000737:chemistry / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C506150:CHGA protein, human; C506134:CHGB protein, human; D053379:Chromogranin A; D053378:Chromogranin B; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D011506:Proteins