Gene sequence of recA+ and construction of recA mutants of Vibrio cholerae.
The recA+ gene of Vibrio cholerae O1 has been cloned, its nucleotide sequence determined and the product characterized. A deletion mutation was constructed in the recA gene and mutants showed the typical sensitivity to UV and to DNA-damaging agents, as well as an inability to mediate homologous DNA recombination. The chromosomal recA deletion mutants in V. cholerae do not show altered virulence in the infant mouse cholera model and are thus ideal strains for use in complementation studies.
Stroeher
U H
UH
Department of Microbiology and Immunology, University of Adelaide, Australia.
Lech
A J
AJ
Manning
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011938:Rec A Recombinases / Q000145:classification / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014734:Vibrio cholerae / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D011938:Rec A Recombinases