Phorbol ester inhibits DNA synthesis induced by interleukin-6 in TSH-pretreated FRTL-5 cells.
Combination of TSH and Interleukin-6 (IL-6) can stimulate DNA synthesis in FRTL-5 cells. Phorbol 12-myristate 13-acetate (PMA) stimulates G0 to G1 transition but inhibits G1 to S transition in these cells. We studied the effect of IL-6 on DNA synthesis in TSH-pretreated FRTL-5 cells in the presence or absence of PMA. IL-6 induced DNA synthesis when it was added to TSH-pretreated cells. Further, PMA inhibited the DNA synthesis induced by IL-6. These data might suggest that in FRTL-5 cells IL-6 can show its mitogenic effect in G1 phase of their cell cycle progression.
Nishiyama
S
S
Department of Laboratory Medicine, Osaka University School of Medicine, Japan.
Takano
T
T
Takada
K
K
Tada
H
H
Amino
N
N
D000818:Animals; D002460:Cell Line / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D016193:G1 Phase; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013961:Thyroid Gland / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*; D000093242:Interleukin-6 Inhibitors
D004247:DNA; D013755:Tetradecanoylphorbol Acetate; D013972:Thyrotropin; D000093242:Interleukin-6 Inhibitors