Restriction endonucleases from Selenomonas ruminantium which recognize and cleave 5'-AT/TAAT-3'.
Two natural isolates from fallow-deer rumen identified as Selenomonas ruminantium were found to produce a restriction endonuclease which we called Sru4DI. This enzyme was isolated from cell extracts by phosphocellulose chromatography. Analysis of the Sru4DI recognition site showed that Sru4DI recognizes the hexanucleotide sequence 5'-AT/TAAT-3' generating 5'dinucleotide protruding ends upon cleavage and thus is a true isoschizomer of vspI, a restriction enzyme isolated from Vibrio sp.
Pristas
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P
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice.
Vanat
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I
Godany
A
A
Javorský
P
P
D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism*; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology*; D008969:Molecular Sequence Data; D012417:Rumen / Q000382:microbiology*
D004262:DNA Restriction Enzymes