title: Sequence of the gene encoding an alkaline serine protease of thermophilic Bacillus smithii.
abstract: The complete nucleotide sequence of the gene encoding an alkaline serine protease from Bacillus smithii has been determined. Degenerate oligodeoxyribonucleotide primers were used to prime the amplification of a 507-bp sequence of the gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction, using as template self-ligated DNA fragments. The deduced amino-acid sequence is compared to serine proteases from B. amyloliquefaciens, B. licheniformis, B. subtilis and Thermus aquaticus.
authors.affiliation: Dipartimento di Genetica e Microbiologia, University of Pavia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Milano
authors.forename: P L
authors.initials: PL
authors.lastname: Manachini
authors.forename: C
authors.initials: C
authors.lastname: Parini
authors.forename: G
authors.initials: G
authors.lastname: Riccardi
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D012697:Serine Endopeptidases