title: Estrogen affects calcitonin gene-related peptide- and methionine-enkephalin-immunoreactive neuron in the female rat preoptic area.
abstract: The effects of estrogen on the neurons of the medial preoptic area of the ovariectomized rat were immunohistochemically investigated using antisera to calcitonin gene-related peptide (CGRP) and methionine-enkephalin (Met-Enk). To visualize CGRP- and Met-Enk-immunoreactive (IR) cell somata, colchicine was injected into the cerebroventricle. CGRP- and Met-Enk-IR neurons were distributed in the medial preoptic nucleus (MPN) but few in the periventricular preoptic nucleus (PPN) in the ovariectomized rat. After estrogen treatment, CGRP immunoreactivity was markedly increased in the PPN and MPN, whereas Met-Enk immunoreactivity was increased in the MPN. These results, along with our previous data, suggest that estrogen accelerates CGRP- and Met-Enk expression in a different manner in the PPN and MPN neurons.
authors.affiliation: Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yuri
authors.forename: M
authors.initials: M
authors.lastname: Kawata
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology / Q000502:physiology*; D004745:Enkephalins / Q000276:immunology / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000187:drug effects / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D010052:Ovariectomy; D011301:Preoptic Area / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000276:immunology / Q000378:metabolism
chemical_list: D004745:Enkephalins; D004967:Estrogens; D011960:Receptors, Estrogen; D004958:Estradiol; D015740:Calcitonin Gene-Related Peptide