Major histocompatibility complex class I related molecules control the development of CD4+8- and CD4-8- subsets of natural killer 1.1+ T cell receptor-alpha/beta+ cells in the liver of mice.
Normal mouse liver contains prominent subsets of CD4+8- and CD4-8- T cell receptor (TCR)-alpha/beta+ cells with intermediate TCR levels. We show here that these cells express the natural killer (NK)1.1 surface antigen and have a restricted TCRV beta repertoire that is highly skewed to V beta 7 and V beta 8. Surprisingly, both CD4+8- and CD4-8- subsets of NK1.1+TCR-alpha/beta+ cells are absent in the liver of beta 2-microglobulin deficient mice, which do not express major histocompatibility complex (MHC) class I or "class I-like" molecules. Analysis of reciprocal radiation bone marrow chimeras established with beta 2-microglobulin deficient and wild-type mice demonstrates that MHC class I expression on radiosensitive (presumably hematopoietic) cells is required for the development of NK1.1+TCR-alpha/beta+ cells in the liver. In the liver of MHC class II deficient mice, the CD4+8- and CD4-8- subsets of NK1.1+TCR-alpha/beta+ cells develop normally. Collectively our data suggest that NK1.1+TCR-alpha/beta+ cells in liver require interaction with a MHC class I-related ligand on hematopoietic cells for their development. This unusual property of liver T cells is shared by a subset of CD4-8-NK1.1+TCR-alpha/beta+ thymocytes, suggesting a common lineage independent of the mainstream of T cell development.
Ohteki
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Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
MacDonald
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HR
D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002455:Cell Division; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000502:physiology*; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology; D008099:Liver / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology
D015704:CD4 Antigens; D016827:CD8 Antigens; D015395:Histocompatibility Antigens Class I; D016693:Receptors, Antigen, T-Cell, alpha-beta