title: Characterization and differentiation of diglycosyl flavonoids by positive ion fast atom bombardment and tandem mass spectrometry.
abstract: This study shows that fast atom bombardment (FAB) in combination with collisional activation and tandem mass spectrometric techniques is useful for the characterization of, and the differentiation between, O-diglycosyl, O-C-diglycosyl and di-C-glycosyl flavonoids. A quick differentiation between the three types of glycosyl flavonoids is possible by simply examining FAB mass spectra. Low-energy product ion spectra of [M+H]+ ions have been used for determining the carbohydrate sequence in O-diglycosyl flavonoids and characterizing the terminal monosaccharide in O-C-diglycosyl flavonoids. The data obtained for per-O-deuterated precursor ion species of O-diglycosyl flavonoids indicate that O-linked hydrogen atoms are involved in the formation of Yn+ ions. High-energy product ion spectra of [M+H]+ ions enable further characterization and differentiation of isomeric di-6,8-C-glycosides.
authors.affiliation: Department of Pharmaceutical Sciences, University of Antwerp, UIA, Belgium.
authors.forename: Q M
authors.initials: QM
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Claeys
mesh_terms: D002240:Carbohydrate Sequence; D005419:Flavonoids / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D005419:Flavonoids