[Immunocytochemical study of mononuclear cells in peripheral blood and cerebrospinal fluid of patients with Japanese B encephalitis].
Using the technique of monoclonal antibody-immunogold-silver staining (M-IGSS), the mononuclear cells in peripheral blood (PB) and cerebrospinal fluid (CSF) were screened for immunoreactivity against JBE virus antigen (JBEV-Ag), interleukin-2 receptor (Tac), class II human leucocyte antigen (HLA-DR) and interferon-r (IFN-r) in 17 ELISA diagnosed JEB patients and 17 control cases with non-inflammatory and non-immunological diseases. It was found that JBEV-Ag+ cells were present in PB (16/17) and CSF (12/17) of the JBE group, but absent in both the control group and another 5 cases with mumps virus meningitis. The percentages of Tac+, HLA-DR+ and IFN-r+ cells in JBE patients were higher than those in controls.
Deng
Y C
YC
Department of Neurology, Xijing Hospital, Xian Fourth Military Medical University.
Su
X C
XC
Feng
Y Q
YQ
D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000378:metabolism*; D016022:Case-Control Studies; D002648:Child; D002681:China; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D015375:Receptors, Interleukin-2 / Q000378:metabolism
D000956:Antigens, Viral; D006684:HLA-DR Antigens; D015375:Receptors, Interleukin-2; D007371:Interferon-gamma