Multicenter evaluation of automated immunoturbidimetric assays for measurement of apolipoproteins A-I and B in serum and plasma.
Results of a multicenter evaluation of automated assays for measurement of apolipoproteins (apo) A-I and B with the Paramax analytical system are reported. Apo A-I and apo B response surface models were used to optimize concentrations of critical assay variables. Overall imprecision for apo A-I controls at concentrations of 1.01-1.61 g/L was 3.7-6.6%; overall imprecision for apo B controls at 1.00-1.61 g/L was 2.8-6.9%. There was no interference in apo A-I measurements. Albumin concentrations > 59 g/L resulted in a negative interference, and collection in sodium heparin caused a positive interference in apo B results. Apo A-I and apo B assays demonstrated acceptable agreement with comparative methods, although the Paramax apo B assays had a negative bias with respect to comparison methods. In 116 healthy individuals, serum apo A-I ranged from 0.97 to 2.05 g/L and serum apo B ranged from 0.51 to 1.32 g/L.
Kahn
S E
SE
Department of Pathology, Loyola University Medical Center, Maywood, IL 60153.
Labbé
R F
RF
Pfadenhauer
E H
EH
Kanonchoff
A D
AD
Joseph
R T
RT
D016632:Apolipoprotein A-I / Q000032:analysis*; D001055:Apolipoproteins B / Q000032:analysis*; D001331:Automation; D006801:Humans; D007118:Immunoassay; D007202:Indicators and Reagents; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012016:Reference Values; D015203:Reproducibility of Results
D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D007202:Indicators and Reagents