title: Experimental study of the pathogenesis of acute acalculous cholecystitis: role of autonomic denervation.
abstract: This study was undertaken to elucidate the role of autonomic denervation in the pathogenesis of acute acalculous cholecystitis. In Experiment I, the gallbladder was denervated by performing either celiac neurotomy (sympathetic denervation) or truncal vagotomy (parasympathetic denervation), or both, in dogs. In Experiment II, 45-min ischemia and 90-min reperfusion of the gallbladder with or without autonomic denervation were performed by simultaneously occluding the middle hepatic artery and superior mesenteric vein. Celiac neurotomy, and truncal vagotomy, or both, did not cause cholecystitis. Sympathetic denervation, however, decreased the amount of mucin in the gallbladder mucosa and parasympathetic denervation caused reduction of the tissue blood flow, as well as the accumulation of lipid peroxide and xanthine oxidase in the gallbladder mucosa. These changes were most remarkable 1-2 weeks after denervation and were alleviated 4 weeks after denervation. Ischemia-reperfusion 2 weeks after denervation caused more severe cholecystitis than ischemia-reperfusion alone. The most severe inflammation developed in animals that received both celiac neurotomy and truncal vagotomy. These results suggest that autonomic denervation alone does not induce acute cholecystitis, but that it plays an important role in the progression of the inflammatory process in ischemia-reperfusion injury.
authors.affiliation: First Department of Surgery, Mie University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tabata
mesh_terms: D000208:Acute Disease; D000818:Animals; D017763:Autonomic Denervation; D001341:Autonomic Nervous System / Q000502:physiology*; D002764:Cholecystitis / Q000209:etiology* / Q000473:pathology; D004285:Dogs; D005260:Female; D005704:Gallbladder / Q000294:innervation* / Q000378:metabolism / Q000473:pathology; D008297:Male; D015427:Reperfusion Injury / Q000150:complications* / Q000473:pathology; D013997:Time Factors