An in-vitro study of the sterol content and toxin production of Fusarium isolates from mycotic keratitis.
Isolates of Fusarium species from 18 patients with keratomycosis were examined for their C-29 and C-31 sterol content and for their capacity to synthesise mycotoxins. All isolates were resistant to azole antifungal agents in vitro and the sterol contents were indistinguishable. In-vitro toxin production was monitored by gas chromatography-mass spectrometry; 13 isolates produced nivalenol, six produced deoxynivalenol, nine gave T-2 toxin and two showed the presence of diacetoxyscirpenol at different time intervals. However, neither sterol content nor toxin production in vitro appeared to be related to the severity of infections observed in patients.
Raza
S K
SK
St John's Institute of Dermatology, UMDS, University of London, St Thomas' Hospital.
Mallet
A I
AI
Howell
S A
SA
Thomas
P A
PA
D000935:Antifungal Agents / Q000494:pharmacology / Q000627:therapeutic use; D004352:Drug Resistance, Microbial; D015821:Eye Infections, Fungal / Q000188:drug therapy / Q000382:microbiology*; D005670:Fusarium / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007634:Keratitis / Q000188:drug therapy / Q000382:microbiology*; D009183:Mycotoxins / Q000096:biosynthesis*; D013261:Sterols / Q000032:analysis*; D016896:Treatment Outcome
D000935:Antifungal Agents; D009183:Mycotoxins; D013261:Sterols