A method for the high efficiency of water-soluble carbodiimide-mediated amidation.
Water-soluble carbodiimides are widely used for carboxyl-amine conjugation. However, extremely variable and low yields, obtained under a variety of conditions, have been a serious problem in the coupling. A simple method, optimizing various parameters of the coupling reaction, in which N-hydroxysuccinimide is included to assist the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride-catalyzed amidation reaction is described. A product yield of up to 90% is routinely achieved.
Sehgal
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Department of Animal Sciences, University of Maryland, College Park 20742.
Vijay
I K
IK
D000577:Amides / Q000737:chemistry*; D000818:Animals; D002384:Catalysis; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry*; D005260:Female; D008321:Mammary Glands, Animal / Q000201:enzymology*; D015394:Molecular Structure; D051381:Rats; D012995:Solubility; D014867:Water / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000302:isolation & purification*
D000577:Amides; D014867:Water; C042059:glucosidase I; D000520:alpha-Glucosidases; D005022:Ethyldimethylaminopropyl Carbodiimide