title: Heterogeneity of IgG antibody responses to cloned Onchocerca volvulus antigens in microfiladermia positive individuals from Esmeraldas Province, Ecuador.
abstract: The prevalence of IgG antibodies to three recombinant O. volvulus antigens, OvMBP/10, OvMBP/11 and OvMBP/29 was determined in a group of 94 microfilaria positive (mf+) individuals resident in the hyperendemic onchocercal area of Esmeraldas Province, Ecuador. Clone OvMBP/11 was the antigen most frequently recognized by patients sera followed by OvMBP/10 and OvMBP/29. When a cocktail of the three recombinant antigens was used the proportion of positive sera increased to 100%. Antibody responses to the fusion partner maltose binding protein (MBP) were low in comparison with those to the cloned antigens and no correlation of responses between individual antigens was observed. The relative level of antibody response to each of the clones in the cocktail varied between individuals. The distribution of IgG responses to OvMBP/11 was bimodal and those to OvMBP/29 and OvMBP/10 were positively and negatively skewed, respectively. When the three recombinant antigens were used in combination this variation was minimized and the pattern of responses showed a normal distribution as was also seen to crude O. volvulus antigen. The cocktail of recombinants thus offers excellent diagnostic sensitivity in combination with the parasite specificity demonstrated previously.
authors.affiliation: Department of Biology, Imperial College of Science, Technology and Medicine, London, UK.
authors.forename: K R
authors.initials: KR
authors.lastname: Trenholme
authors.forename: T I
authors.initials: TI
authors.lastname: Tree
authors.forename: A J
authors.initials: AJ
authors.lastname: Gillespie
authors.forename: R
authors.initials: R
authors.lastname: Guderian
authors.forename: R M
authors.initials: RM
authors.lastname: Maizels
authors.forename: J E
authors.initials: JE
authors.lastname: Bradley
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology*; D001483:Base Sequence; D002352:Carrier Proteins / Q000276:immunology; D004484:Ecuador / Q000453:epidemiology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D058619:Maltose-Binding Proteins; D008875:Middle Aged; D008969:Molecular Sequence Data; D017181:Onchocerca volvulus / Q000276:immunology*; D009855:Onchocerciasis / Q000453:epidemiology / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002352:Carrier Proteins; D007074:Immunoglobulin G; D058619:Maltose-Binding Proteins; D011994:Recombinant Proteins