cDNA cloning and functional expression of a human monocyte chemoattractant protein 1 receptor.
A novel human G-protein-coupled seven-transmembrane-type receptor gene, 24-1, has been cloned from THP-1 cells. The 24-1 was 56% and 34% identical to the macrophage inflammatory protein-1 alpha (MIP-1 alpha) receptor and the interleukin-8 (IL-8) receptors at the amino acid level, respectively. To examine the ligand specificity of the receptor, we have established a stable transfectant of this gene with human kidney 293 cells. Monocyte chemoattractant protein-1 (MCP-1) induced a rapid increase of Ca++ influx in the 24-1-transfectant, while other C-C chemokines tested did not. This indicates that the 24-1 encodes the human MCP-1 receptor.
Yamagami
S
S
Pharmaceutical Discovery Research Laboratories, Teijin Institute for Biomedical Research, Tokyo, Japan.
Tokuda
Y
Y
Ishii
K
K
Tanaka
H
H
Endo
N
N
D000595:Amino Acid Sequence; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000494:pharmacology; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000502:physiology; D015870:Gene Expression; D006801:Humans; D007668:Kidney; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D054390:Receptors, CCR2; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine / Q000737:chemistry / Q000235:genetics*; D014162:Transfection
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D054390:Receptors, CCR2; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D019204:GTP-Binding Proteins; D002118:Calcium