IgG transport across the gut of the suckling opossum (Monodelphis domestica).
We investigated IgG transport across the gut of suckling opossums to see whether it is likely to be Fc gamma R-mediated. Enterocytes isolated from the proximal and distal regions of the small intestine of suckling aged 12-52 days, and reacted with indicator SRBC at pH 6.0 or 7.2, bound opossum IgG in rosette assays. Considerable overall variation was observed in the numbers of enterocytes forming rosettes. No binding was seen with rabbit IgG at these ages, or with opossum and rabbit IgG when enterocytes were obtained from opossums aged 55-73 days. Opossum anti-SRBC antibody (IgG) fed to sucklings at 52 days and earlier (but not later) could subsequently be detected in the serum. However, rabbit anti-SRBC antibody (IgG) could not be detected in the blood serum when fed to sucklings of any age. Fluorescent tracing of FITC-labelled opossum and rabbit IgG fed to suckling opossums, and of endogenous opossum IgG, pointed to transport of the homologous IgG occurring across gut enterocytes of the proximal region. These results suggest that IgG is recognised and transcytosed by specific Fc gamma Rs present on opossum enterocytes prior to weaning.
Wild
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Department of Biology, School of Biological Sciences, University of Southampton, UK.
Burrows
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TD
Brand
J
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D000367:Age Factors; D000818:Animals; D000833:Animals, Suckling; D001693:Biological Transport, Active; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000378:metabolism*; D007421:Intestine, Small / Q000276:immunology*; D009893:Opossums / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D017452:Receptors, IgG / Q000378:metabolism; D013045:Species Specificity
D007074:Immunoglobulin G; D017452:Receptors, IgG