title: Canine model of obstructive sleep apnea: model description and preliminary application.
abstract: This report describes a canine model of obstructive sleep apnea (OSA) developed in our laboratory and the results of its preliminary short-term application. Healthy adult dogs were prepared with a tracheostomy and with implanted electroencephalographic and nuchal electromyographic recording electrodes. A silent occlusion valve was attached to the outer end of the endotracheal tube. The electroencephalogram and electromyogram were monitored continuously by a computer that determined sleep-wake state using software developed in our laboratory. At a predetermined time (e.g., 12 s) after each sleep onset, a signal was transmitted from the computer to the valve controller, resulting in airway occlusion. When the dog aroused from sleep, the occlusion was released. These events therefore mimic those that occur in human OSA. Successful operation of the model was confirmed during 5-day continuous trials in two dogs. During the trials, the dogs became increasingly somnolent both by behavioral observation and objective measurement. The frequency of occlusions increased, and measures of apnea severity, including apnea duration and end-apneic arterial oxygen saturation, worsened. We conclude that this experimental model of repeated airway occlusion during sleep provides a potentially powerful tool for investigating the sequelae of OSA.
authors.affiliation: Department of Medicine, University of Toronto, Ontario, Canada.
authors.forename: R J
authors.initials: RJ
authors.lastname: Kimoff
authors.forename: H
authors.initials: H
authors.lastname: Makino
authors.forename: R L
authors.initials: RL
authors.lastname: Horner
authors.forename: L F
authors.initials: LF
authors.lastname: Kozar
authors.forename: F
authors.initials: F
authors.lastname: Lue
authors.forename: A S
authors.initials: AS
authors.lastname: Slutsky
authors.forename: E A
authors.initials: EA
authors.lastname: Phillipson
mesh_terms: D000402:Airway Obstruction / Q000503:physiopathology* / Q000523:psychology; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D004195:Disease Models, Animal; D004285:Dogs; D004567:Electrodes, Implanted; D004569:Electroencephalography; D004576:Electromyography; D010101:Oxygen Consumption / Q000502:physiology; D012890:Sleep / Q000502:physiology; D012891:Sleep Apnea Syndromes / Q000503:physiopathology* / Q000523:psychology; D012895:Sleep, REM / Q000502:physiology; D013686:Telemetry