title: Peripheral and intrasplenic platelet kinetics and bone marrow megakaryopoiesis in alpha-2b-interferon treated hairy cell leukemia.
abstract: In eight patients with previously untreated hairy cell leukemia (HCL), by using 111In-labelled platelets and megakaryocyte quantitation, the splenic platelet pooling and the platelet production rate (P) were evaluated before and during alpha-2b-interferon (IFN) treatment. Both before and after 8 months of IFN therapy the spleen was shown to pool a sizeable amount of the total body platelet mass. The average splenic platelet pools, prior to and after 8 months of IFN, were 58 +/- 17 and 47 +/- 11%, respectively. At the time when treatment was initiated, the patients were heterogeneous as regards the spleen size, platelet kinetics, and the bone marrow morphology. Three patients had values for P below the 95th percentile for a group of healthy control subjects; following IFN therapy they displayed a substantial increase in P. In three other HCL patients, with the largest spleens, the pre-treatment P was normal, or slightly above the values seen for the control subjects. In these patients, changes in splenic platelet pool size, blood volume, and platelet mean life-span accounted for the increase in platelet count observed in response to IFN. The mean megakaryocyte number and volume per microliter bone marrow increased during IFN therapy, while the mean P remained slightly reduced. It is concluded that splenic platelet pooling would explain the previously described difference in platelet counts between splenectomized and non-splenectomized patients treated with IFN.
authors.affiliation: Department of Medicine, Sahlgren's Hospital, University of Göteborg, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Wadenvik
authors.forename: I
authors.initials: I
authors.lastname: Braide
authors.forename: B
authors.initials: B
authors.lastname: Ridell
authors.forename: J
authors.initials: J
authors.lastname: Kutti
authors.forename: S
authors.initials: S
authors.lastname: Jacobsson
authors.forename: P
authors.initials: P
authors.lastname: Revesz
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology*; D002452:Cell Count; D002470:Cell Survival; D006410:Hematopoiesis; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000627:therapeutic use*; D007700:Kinetics; D007943:Leukemia, Hairy Cell / Q000097:blood* / Q000628:therapy*; D008297:Male; D008533:Megakaryocytes / Q000473:pathology*; D008875:Middle Aged; D010976:Platelet Count; D011994:Recombinant Proteins; D013154:Spleen / Q000473:pathology*
chemical_list: D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins