Evaluation of allergenic potency by REAST inhibition. A new tool for the standardization of allergenic extracts.
The potency of allergenic extracts can be determined in vitro by RAST inhibition, and this has become the preferred method for the standardization of allergens. A disadvantage of this technique is the impossibility of obtaining data about allergens bound to the solid phase, i.e., the counterpart of the inhibiting extract. The REAST (reverse enzyme allergosorbent test) is based on the capture of IgE by a specific antibody bound to microtiter wells, the reaction of captured IgE with biotinylated allergen and the development of a colour reaction by subsequent addition of streptavidin-peroxidase and chromogenic substrate. The addition of an allergen extract in a dose-response fashion competes with the biotinylated allergen and inhibits the test. In the present study REAST inhibition has been evaluated with Dermatophagoides pteronyssinus, Parietaria judaica and mixed grass pollen extracts. The correlation of REAST inhibition with RAST inhibition and both intra-assay and inter-assay reproducibility have been evaluated. REAST inhibition is a potentially valuable new tool for the standardization of allergenic extracts.
Falagiani
P
P
Research Department, Laboratorio Farmaceutico Lofarma S.R.l., MIlan, Italy.
Mistrello
G
G
Rapisarda
G
G
Festa
A
A
Cislaghi
C
C
Zanoni
D
D
D000485:Allergens / Q000276:immunology*; D000818:Animals; D001667:Binding, Competitive; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008925:Mites / Q000276:immunology; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000276:immunology; D011852:Radioallergosorbent Test; D012015:Reference Standards; D015203:Reproducibility of Results
D000485:Allergens; D007073:Immunoglobulin E