Bovine neutrophil chemiluminescence is preferentially stimulated by homologous IL-1, but inhibited by the human IL-1 receptor antagonist.
Previous studies have indicated that certain types of bovine cells preferentially respond to bovine interleukin-1 (IL-1) compared with IL-1 from other mammalian species. In this study, we demonstrate that bovine neutrophils undergo a substantially greater luminol-dependent chemiluminescence response to bovine IL-1 beta than to human IL-1 alpha, human IL-1 beta, or murine IL-1 alpha. Likewise, approximately 1000-fold less bovine IL-1 beta than human IL-1 beta was required to prime bovine neutrophils for an enhanced luminol-dependent chemiluminescence response to opsonized zymosan particles. A recombinant human IL-1 receptor antagonist was a potent inhibitor of bovine neutrophil stimulation and priming by homologous and heterologous IL-1. These data indicate a hierarchy amongst members of the IL-1 family in terms of their relative abilities to modulate the functional activation of bovine neutrophils.
Sample
A K
AK
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
Czuprynski
C J
CJ
D000818:Animals; D002417:Cattle; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000494:pharmacology*; D008163:Luminescent Measurements; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000494:pharmacology; D016897:Respiratory Burst / Q000502:physiology; D012795:Sialoglycoproteins / Q000494:pharmacology*; D015054:Zymosan
C507763:IL1RN protein, human; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D015054:Zymosan