[Measurement of the number of single-strand breaks in DNA of irradiated leukemia P-388 using alkaline denaturation].
Methods, used for indirect determination of single-thread breaks' quantity in DNA, based on it's denaturation in alkali medium and further determination of double-thread DNA quantity by it's fluorescence has been adapted to the measuring of thread disturbances on leukemia P-388 cells. Influence of cells' and alkali concentrations and the time of denaturation of quantity of double-thread DNA has been investigated.
Fomin
V V
VV
Borisov
M B
MB
Zaĭtsev
A V
AV
Blokhin
D Iu
DIu
D000818:Animals; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D007941:Leukemia P388 / Q000235:genetics*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009691:Nucleic Acid Denaturation; D012972:Sodium Hydroxide; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D012972:Sodium Hydroxide
Izmerenie kolichestva odnonitevykh razryvov v DNK obluchennykh kletok leĭkoza P-388 metodom shchelochnoĭ denaturatsii.