title: [Analysis of hydroxypyridine cross-links of collagen from human rib cartilage].
abstract: Ion-paired reversed-phase high performance liquid chromatography (HPLC) has been used for the analysis of the content of mature collagen crosslinks--hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) in biopsy specimens of human rib cartilage from healthy donors (n = 14) and patients with inherited diseases of the connective tissue complicated with funnel chest (n = 17). Analysis of normal tissues reveal the presence of LP (alongside with HP) in embryonal rib cartilage. LP has been found in the rib cartilage of 4 out of 6 patients with funnel chest (FC) associated with Ehlers-Danlos syndrome (EDS); with no signs of this pathology detected in individuals with isolated FC. In rib cartilage of 2 patients with recurrent isolated FC LP has been discovered alongside with the presence of type I collagen. A significant increase of LP content in rib cartilage in a child with clinical phenotype of EDS type VI has been discovered.
authors.forename: N V
authors.initials: NV
authors.lastname: Borisova
authors.forename: A Ia
authors.initials: AIa
authors.lastname: Pokrovskaia
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Zakharova
authors.forename: K D
authors.initials: KD
authors.lastname: Krasnopol'skaia
authors.forename: S A
authors.initials: SA
authors.lastname: Poliudov
mesh_terms: D000293:Adolescent; D000596:Amino Acids / Q000737:chemistry*; D002356:Cartilage / Q000737:chemistry*; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000737:chemistry*; D003240:Connective Tissue Diseases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D046911:Macromolecular Substances; D011725:Pyridines / Q000737:chemistry*; D012016:Reference Values; D012272:Ribs
chemical_list: D000596:Amino Acids; D046911:Macromolecular Substances; D011725:Pyridines; C014047:hydroxypyridines; C015484:pyridinoline; C036020:deoxypyridinoline; D003094:Collagen
vernacular_title: Analiz gidroksipiridinovykh sshivok kollagena rebernogo khriashcha cheloveka.