title: Diagnosis of acute hepatitis E infection utilizing enzyme immunoassay.
abstract: Acute hepatitis E infection was diagnosed in a Pakistani immigrant admitted to the University of Illinois Hospital. Utilizing enzyme immunoassay (EIA) tests, specific IgG and IgM class antibodies to three different epitopes of hepatitis E virus (HEV) were detected 12 weeks after the onset of illness and in the early convalescent stage. Sixteen months after the onset of hepatitis, IgM anti-HEV was no longer detectable. Low levels of IgG class anti-HEV antibodies continued to be detected. We demonstrate the utility of the EIA HEV assay to diagnose prospectively acute HEV infection.
authors.affiliation: Section of Digestive and Liver Diseases, University of Illinois at Chicago.
authors.forename: L J
authors.initials: LJ
authors.lastname: DeGuzman
authors.forename: D L
authors.initials: DL
authors.lastname: Pitrak
authors.forename: G J
authors.initials: GJ
authors.lastname: Dawson
authors.forename: D C
authors.initials: DC
authors.lastname: Shin
authors.forename: T J
authors.initials: TJ
authors.lastname: Holzer
authors.forename: T J
authors.initials: TJ
authors.lastname: Layden
mesh_terms: D000208:Acute Disease; D000328:Adult; D006508:Hepatitis Antibodies / Q000032:analysis*; D016751:Hepatitis E / Q000175:diagnosis* / Q000276:immunology; D016752:Hepatitis E virus / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male
chemical_list: D006508:Hepatitis Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M