title: Cloning and sequence analysis of a hamster liver cDNA encoding a novel putative carboxylesterase.
abstract: A full-length cDNA encoding for a putative carboxylesterase was isolated from a hamster liver cDNA library. The cDNA consisting of 1911 base pairs contained an open reading frame of 1683 base pairs encoding for a polypeptide of 561 amino-acid residues, including 27 N-terminal amino-acid residues for signal peptide. The deduced amino-acid sequence of the cDNA is in 67% homology with the amino-acid sequence of rabbit form 2 carboxylesterase, which has not yet been cloned. It also had many structural features highly conserved among carboxylesterase isozymes.
authors.affiliation: Department of Toxicology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sone
authors.forename: M
authors.initials: M
authors.lastname: Isobe
authors.forename: E
authors.initials: E
authors.lastname: Takabatake
authors.forename: C Y
authors.initials: CY
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000737:chemistry* / Q000302:isolation & purification; D015723:Gene Library; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase