title: KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore.
abstract: We have used in vitro motility assays to investigate the mechanism of kinetochore function in the budding yeast Saccharomyces cerevisiae. Functional centromeric DNA plus a tripartite centromere binding protein complex, CBF3, was found to be necessary but not sufficient for in vitro kinetochore activity. A fourth required component was identified as the motor protein Kar3p, a previously reported yeast kinesin known to be involved in karyogamy and mitosis. Our data support genetic evidence suggesting that Kar3p is a kinetochore-associated motor and imply that CBF3 plays a regulatory role in kinetochore function.
authors.affiliation: Department of Biological Sciences, University of California, Santa Barbara 93106.
authors.forename: K
authors.initials: K
authors.lastname: Middleton
authors.forename: J
authors.initials: J
authors.lastname: Carbon
mesh_terms: D002503:Centromere / Q000502:physiology*; D015825:Chromosomes, Fungal; D004268:DNA-Binding Proteins / Q000502:physiology*; D005656:Fungal Proteins / Q000502:physiology*; D016547:Kinesins / Q000502:physiology*; D018386:Kinetochores; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C092348:CEP3 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C063504:KAR3 protein, S cerevisiae; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D016547:Kinesins