title: Chromosome localization and orientation of the simple sequence repeat of human satellite I DNA.
abstract: The predominant chromosomal locations of human satellite I DNA were detected using fluorescent in situ hybridization (FISH). Synthetic deoxyoligonucleotides designed from consensus sequences of the simple sequence repeats of satellite 1 were used as probes. The most abundant satellite I repeat, the -A-B-A-B-A- form, is located at the pericentromeric regions of chromosomes 3, 4, 13, 14, 15, 21, and 22. The less abundant -B-B-B-form was not detected on chromosome 4, but was present at all the other locations. A variation of FISH that allows strand-specific hybridization of single-stranded probes (CO-FISH) determined that the human satellite I sequences are predominantly arranged in head-to-tail fashion along the DNA strand.
authors.affiliation: Life Sciences Division, Los Alamos National Laboratory, NM 87545.
authors.forename: J
authors.initials: J
authors.lastname: Meyne
authors.forename: E H
authors.initials: EH
authors.lastname: Goodwin
authors.forename: R K
authors.initials: RK
authors.lastname: Moyzis
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D016384:Consensus Sequence / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008969:Molecular Sequence Data; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D004276:DNA, Satellite; D011089:Polydeoxyribonucleotides