Time course of cholinergic and monoaminergic changes in rat brain after immunolesioning with 192 IgG-saporin.
192 IgG-saporin, an immunotoxin targeted at the low affinity NGF receptor, was infused into the lateral ventricle of rat brain. Three days and one week post lesion, choline acetyltransferase activity was markedly decreased in cortex, hippocampus, olfactory bulbs, and septum (brain regions innervated by the cholinergic neurons of the basal forebrain) with no change in cerebellum, striatum or pons. Measurement of monoamine levels revealed increases in HVA, DOPAC and dopamine, primarily in the olfactory bulbs at the 28-day time point only, suggesting a compensation for cholinergic inactivity. High levels of basal forebrain cholinergic lesioning can be obtained with this immunotoxin with minimal or no effects on monoaminergic or other cholinergic systems.
Waite
J J
JJ
Department of Neurosciences, University of California, San Diego 92093.
Wardlow
M L
ML
Chen
A C
AC
Lappi
D A
DA
Wiley
R G
RG
Thal
L J
LJ
D000818:Animals; D015306:Biogenic Monoamines / Q000276:immunology / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D001923:Brain Chemistry / Q000187:drug effects*; D002795:Choline O-Acetyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007074:Immunoglobulin G / Q000633:toxicity; D000922:Immunotoxins / Q000633:toxicity*; D007276:Injections, Intraventricular; D007700:Kinetics; D008297:Male; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344; D017475:Receptors, Nerve Growth Factor / Q000276:immunology; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins
D015306:Biogenic Monoamines; D007074:Immunoglobulin G; D000922:Immunotoxins; D010940:Plant Proteins; D017475:Receptors, Nerve Growth Factor; D054789:Ribosome Inactivating Proteins, Type 1; D002795:Choline O-Acetyltransferase; D009699:N-Glycosyl Hydrolases; D000076985:Saporins