The effect of three cryoprotective diluents on the in vitro transcription of ram sperm cells.
The effect of three different solvents was investigated in run-on experiments, using purified ram sperm nuclei. The best cryoprotective diluent proved to be Nagase-Niwa, which had the most positive effect on transcription. The kinetic data of incorporation of 32P-ATP in pre-mRNA indicated that there was a stepwise increase in the level of transcription during the first 20 min of incubation, being lowest in the control and highest in the presence of the diluent Nagase-Niwa.
Miteva
K
K
Institute of Biology and Immunology, and Reproduction and Development of Organisms, Bulgarian Academy of Science, Sofia.
Goncharova-Peinova
I
I
Zlatarev
S
S
Pironcheva
G
G
Russev
G
G
D000818:Animals; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D012756:Sheep; D012997:Solvents / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
D003451:Cryoprotective Agents; D012997:Solvents