title: Transformation of wheat protoplasts mediated by cationic liposome and regeneration of transgenic plantlets.
abstract: E. coli plasmid pCGN1055 containing the hygromycin phosphotransferase (Hpt) gene under the control of the plant promoter was introduced into wheat protoplasts by cationic liposome (lipofectin)-mediated transformation, and transgenic albino plantlets were obtained. The transformation frequencies were 6.0% and 8.8% according to hygromycin resistance and DNA molecular hybridization. The assay of Hpt enzyme activity and Southern blot indicated the foreign gene existed and expressed in transgenic plantlets.
authors.affiliation: Institute of Genetics, Chinese Academy of Sciences, Beijing.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhu
authors.forename: B
authors.initials: B
authors.lastname: Sun
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: G
authors.initials: G
authors.lastname: Xiao
authors.forename: X
authors.initials: X
authors.lastname: Li
mesh_terms: D002412:Cations; D008081:Liposomes; D010714:Phosphatidylethanolamines; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D030821:Plants, Genetically Modified; D011523:Protoplasts; D012038:Regeneration; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
chemical_list: D002412:Cations; D008081:Liposomes; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase