title: SCM2, a tryptophan permease in Saccharomyces cerevisiae, is important for cell growth.
abstract: SCM2, a novel gene encoding a yeast tryptophan permease, was cloned as a high-copy-number suppressor of cse2-1. The cse2-1 mutation causes cold sensitivity, temperature sensitivity and chromosome missegregation. However, only the cold-sensitive phenotype of cse2-1 cells is suppressed by SCM2 at high copy. SCM2 is located on the left arm of yeast chromosome XV, adjacent to SUP3 and encodes a 65 kDa protein that is highly homologous to known amino acid permeases. Four out of five disrupted scm2 alleles (scm2 delta 1-delta 4) cause slow growth, whereas one disrupted allele (scm2 delta 5) is lethal. Cells with both the scm2 delta 1 and trp1-delta 101 mutations exhibit a synthetic cold-sensitive phenotype and grow much more slowly at the permissive temperature than cells with a single scm2 delta 1 or trp1-delta 101 mutation. A region of the predicted SCM2 protein is identical to the partial sequence recently reported for the yeast tryptophan permease TAP2, indicating that SCM2 and TAP2 probably encode the same protein.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003-4505.
authors.forename: X H
authors.initials: XH
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Xiao
authors.forename: M
authors.initials: M
authors.lastname: Fitzgerald-Hayes
mesh_terms: D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems; D001483:Base Sequence; D001692:Biological Transport; D002455:Cell Division / Q000235:genetics; D002874:Chromosome Mapping; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D016153:Genes, Suppressor / Q000235:genetics*; D056892:Mediator Complex; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014364:Tryptophan / Q000378:metabolism
chemical_list: D026905:Amino Acid Transport Systems; C082275:CSE2 protein, S cerevisiae; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D056892:Mediator Complex; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C429825:TAT2 protein, S cerevisiae; D014157:Transcription Factors; C035131:TnaB protein, E coli; D014364:Tryptophan