Identification of an ATP-sensitive K+ channel in spiny neurons of rat caudate nucleus.
On the somata of GABAergic spiny neurons in the caudate nucleus of the rat an ATP-sensitive K+ channel (KATP-channel) was identified. The KATP-currents in cell-attached patches were activated both by energy-depleting conditions (200 microM cyanide) and by diazoxide (300 microM) and were reversibly blocked by tolbutamide (EC50 = 5 microM). In inside-out patch membranes both ATP (1 mM) and its non-hydrolyzable analog AMP-PNP (adenylylimidodiphosphate; EC50 = 27 microM) reversibly inhibited channel activity. These results demonstrate that the KATP-channel in spiny neurons displays properties characteristic of the KATP-channel in hippocampal, neocortical and nigral neurons and in pancreatic beta-cells.
Schwanstecher
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C
Institut für Pharmakologie und Toxikologie, Universität Göttingen, Germany.
Panten
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U
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000266:Adenylyl Imidodiphosphate / Q000494:pharmacology; D000818:Animals; D002421:Caudate Nucleus / Q000166:cytology / Q000378:metabolism*; D003486:Cyanides / Q000494:pharmacology; D003981:Diazoxide / Q000494:pharmacology; D004594:Electrophysiology; D005260:Female; D008297:Male; D009474:Neurons / Q000145:classification / Q000378:metabolism*; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D014044:Tolbutamide / Q000494:pharmacology
D003486:Cyanides; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D000266:Adenylyl Imidodiphosphate; D000255:Adenosine Triphosphate; D014044:Tolbutamide; D003981:Diazoxide