Unusual immunocytochemical and ultrastructural features of synovial fluid cells in multicentric reticulohistiocytosis. A case report.
We present the first report in which cells in synovial fluid from a patient with multicentric reticulohistiocytosis (MRH) were studied by immunocytochemistry for correlation with routine light and electron microscopy. MRH cells stained predominantly for lymphocyte-related surface antigens and not for the monocyte marker LEU M3 (CD14). These findings suggest a lymphocytic origin of MRH cells and not a histiocytic origin, as previously suggested. In addition, large numbers of membrane-bound, electron-dense, secretory-type granules were found ultrastructurally in the cytoplasm of these cells.
Samaan
S S
SS
Rheumatology-Immunology Center, Veterans Administration Medical Center, Philadelphia, Pennsylvania 19104.
Schumacher
H R
HR
Villanueva
T
T
Levin
R
R
Atkinson
B F
BF
D000911:Antibodies, Monoclonal; D001707:Biopsy, Needle; D015616:Histiocytosis, Non-Langerhans-Cell / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D013582:Synovial Fluid / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000473:pathology
D000911:Antibodies, Monoclonal