title: The organization of the beta-globin gene of the bivalve mollusc Anadara trapezia and its evolutionary relationship to other invertebrate and vertebrate globin genes.
abstract: Three pairs of oligonucleotide primers based on partial DNA and amino acid sequences were used in a combination of PCR experiments to amplify the beta-globin gene of the bivalve mollusc Anadara trapezia. The sequence of 2,139 bp presented contains the whole of the beta-globin gene with the exception of the 5' flanking sequence. This gene possesses the three-exon-and-two-intron gene structure typical of vertebrate globin genes but the lengths of the introns (762 bp and 690 bp, respectively) are only approximately half the size of those present in a beta-variant gene previously characterized from this organism. The encoded amino acid sequence shows two changes when compared to the previously published amino acid sequence.
authors.affiliation: School of Biochemistry and Molecular Genetics, University of New South Wales, Kensington, Australia.
authors.forename: N T
authors.initials: NT
authors.lastname: Nassif
authors.forename: W K
authors.initials: WK
authors.lastname: Glenn
authors.forename: A G
authors.initials: AG
authors.lastname: Mackinlay
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D005091:Exons; D014644:Genetic Variation; D005914:Globins / Q000235:genetics*; D007438:Introns; D007448:Invertebrates / Q000235:genetics*; D008969:Molecular Sequence Data; D008974:Mollusca / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D014714:Vertebrates / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005914:Globins; D004247:DNA