title: Flow cytometric detection of chromosome abnormalities by measuring centromeric index, DNA content, and DNA base composition.
abstract: This paper highlights two improvements of the on-line centromeric index (CI) analysis for the detection of chromosome abnormalities. On-line CI versus DNA content analysis of an EBV-transformed cell line, with a deletion (11)(p13p15.1), of a patient with aniridia and Wilms' tumour demonstrates the first improvement of the method of on-line CI analysis for flow karyotyping and sorting; a reciprocal translocation, insertion, or deletion can, when the cell type contains not more than a few of these types of abnormalities, be traced to the p-arm or q-arm of the relevant chromosome. On-line CI analysis was also performed with chromosomes isolated from a transitional cell carcinoma of the bladder. Cytogenetic analysis of this cell line showed numerous chromosomal abnormalities. Chromosomes of this cell line were also karyotyped by bivariate flow cytometry using a different set of parameters: Hoechst 33,258 fluorescence intensity (HOfl) versus chromomycin A3 fluorescence intensity (CAfl). A comparison of these results reveals the second improvement of the CI method for the detection of chromosome abnormalities; bivariate analysis of CI versus propidium fluorescence (PIfl) are complementary to bivariate analysis of HOfl versus CAfl. Chromosomes with distributions that fuse together in the HO/CA flow karyotype may be distinguished as individual peaks on the basis of their CI values.
authors.affiliation: Laboratory for Radiobiology, University of Amsterdam, The Netherlands.
authors.forename: W
authors.initials: W
authors.lastname: Rens
authors.forename: G A
authors.initials: GA
authors.lastname: Boschman
authors.forename: J M
authors.initials: JM
authors.lastname: Hoovers
authors.forename: E M
authors.initials: EM
authors.lastname: Manders
authors.forename: R M
authors.initials: RM
authors.lastname: Slater
authors.forename: J
authors.initials: J
authors.lastname: Stap
authors.forename: J A
authors.initials: JA
authors.lastname: Aten
mesh_terms: D015783:Aniridia / Q000235:genetics; D001482:Base Composition; D002295:Carcinoma, Transitional Cell / Q000235:genetics; D002461:Cell Line, Transformed; D002503:Centromere / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D003627:Data Interpretation, Statistical; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics; D009396:Wilms Tumor / Q000235:genetics; D014960:X Chromosome
chemical_list: D004273:DNA, Neoplasm; D004247:DNA