Determination of sterol content in different food samples by capillary gas chromatography.
An accurate method for the determination of sterols by capillary gas chromatography was developed and applied to the analysis of food. The procedure includes the following steps: dichloromethane-methanol (2:1, v/v) lipid extraction, saponification at 80 degrees C and separation of the unsaponifiable matter with cyclohexane, derivatization to form trimethylsilyl ethers and gas chromatography using 5 alpha-cholestane as the internal standard. The method shows good accuracy, precision and sensitivity and is suitable for the determination of sterols in food.
Rodríguez-Palmero
M
M
Department de Ciències Fisiològiques i de la Nutrició, Facultat de Farmàcia, Universitat de Barcelona, Spain.
de la Presa-Owens
S
S
Castellote-Bargallo
A I
AI
López Sabater
M C
MC
Rivero-Urgell
M
M
de la Torre-Boronat
M C
MC
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D005399:Fishes; D005504:Food Analysis; D006801:Humans; D011200:Poultry; D013261:Sterols / Q000032:analysis*; D014675:Vegetables
D013261:Sterols