Maturational changes in superoxide anion production of rabbit alveolar macrophages.
We investigated the effect of maturation on the superoxide anion production of rabbit alveolar macrophages by endothelin-1, N-formyl-methionyl-leucyl-phenylalanine, and phorbol 12-myristate 13-acetate. The O2- production by phorbol 12-myristate 13-acetate in young (0.2-0.3 kg) rabbits was as high as that in adult (3.0-3.5 kg) rabbits. However, when alveolar macrophages were stimulated by N-formyl-methionyl-leucyl-phenylalanine or preincubated with endothelin-1 before phorbol 12-myristate 13-acetate stimulation, O2- production of young rabbits was relatively weak compared to adult rabbits. The present data indicate that the ability of O2- production is sufficiently developed with a chemotactic peptide which directly stimulates protein kinase C.
Kojima
T
T
Department of Pediatrics, Kansai Medical University, Osaka, Japan.
Hattori
K
K
Fujiwara
T
T
Hirata
Y
Y
Kobayashi
Y
Y
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D016232:Endothelins / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D008163:Luminescent Measurements; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D011719:Pyrazines / Q000494:pharmacology; D011817:Rabbits; D016897:Respiratory Burst; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D016232:Endothelins; D007093:Imidazoles; D011719:Pyrazines; D013481:Superoxides; C063412:2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-alpha)pyrazin-3-one; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013755:Tetradecanoylphorbol Acetate