Protein kinase C delta- and epsilon-subspecies in rat central nervous tissue; differential distribution and phorbol ester-induced redistribution in synaptosomes.
Distribution of the delta- and epsilon-subspecies of protein kinase C (PKC) in rat central nervous tissues was semiquantitated by an immunoblot analysis. The epsilon-subspecies, which is expressed predominantly in the brain, was abundant in hippocampus and cerebral cortex among rat brain areas, whereas the delta-subspecies, which is expressed ubiquitously in many tissues, showed no significant difference among tissue areas tested. Unlike other subspecies, the delta-subspecies was primarily associated with particulate fractions. Subcellular fractionation analysis revealed that both the epsilon- and delta-subspecies were enriched in the synaptosomal P2 fraction. Treatment with 12-O-tetradecanoyl-phorbol 13-acetate (TPA) induced translocation of the epsilon-subspecies from cytosolic fraction to membrane fraction. In contrast, upon treatment with TPA, the delta-subspecies normally bound to membrane fraction was released into cytosol.
Matsushima
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Department of Biochemistry, Kobe University School of Medicine, Japan.
Nakamura
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D000818:Animals; D001921:Brain / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013574:Synaptosomes / Q000187:drug effects / Q000201:enzymology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D007527:Isoenzymes; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate