Effect of traditional Chinese medicine (sairei-to) on monoclonal antibody-induced proteinuria in rats.
The effects of traditional Chinese medicine (Sairei-to) on monoclonal antibody (mAb) inducing proteinuria were examined. Four hundred, 200 and 100 mg/kg body weight (BW) of Sairei-to and phosphate-buffered saline (PBS) as a control were injected intraperitoneally into four groups of female Wistar rats every day from 5 days before intravenous injection of mAb to the end of the experimental period. The amount of urinary protein excretion was significantly suppressed in roughly a dose-dependent manner. For example, 116.6 +/- 89.7 mg/day of proteinuria was observed in control groups compared to 4.2 +/- 15.2 mg/day in the 400 mg/kg BW of Sairei-to treated group 2 days after mAb injection (P < 0.01). Statistically significant (P < 0.01) differences were again observed in a repeat experiment (122.1 +/- 53.7 vs 10.2 +/- 10.1 mg/day on the 2nd day) without affecting the glomerular filtration rate. No significant difference was recognized between the total amount of mAb bound to glomeruli 1 h after mAb injection in Sairei-to-treated and non-treated rats, indicating that Sairei-to pretreatment has no effects on the number or quality of antigenic molecules. The effect of Sairei-to on a non-immunological model of proteinuria was also examined. No significant reduction of proteinuria by similar Sairei-to treatment was observed in aminonucleoside of puromycin nephropathy. The authors conclude that mAb-induced proteinuria in rats is significantly suppressed by the traditional Chinese medicine, Sairei-to.
Kawachi
H
H
Department of Immunology, Niigata University, School of Medicine, Japan.
Takashima
N
N
Orikasa
M
M
Oite
T
T
Shimizu
F
F
D000818:Animals; D000911:Antibodies, Monoclonal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D005455:Fluorescent Antibody Technique; D009393:Nephritis / Q000139:chemically induced / Q000188:drug therapy; D011507:Proteinuria / Q000188:drug therapy* / Q000276:immunology; D011692:Puromycin Aminonucleoside; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D004365:Drugs, Chinese Herbal; C080903:sairei-to; D011692:Puromycin Aminonucleoside