Parotin subunit and its synthetic peptide possess interleukin 1-like activity and exert stimulating effects on liver cells and brain cells.
We have investigated whether parotin subunit (PS) and its partial synthetic peptide (P-10.2: TDDTAIVLLK), possess interleukin 1 (IL-1)-like activities, and act on cell lines other than lymphocytes. When Chang liver cells were cultured with P-10.2, PS or IL-1, P-10.2 and PS augmented the growth of Chang liver cells. On the other hand, IL-1 enhanced the growth of Change liver cells at 1 day of the initial culture and subsequently failed to enhance during at least 4-day incubation. Next, effects of P-10.2 and PS on the growth of Alexander cells and MH134 were investigated. The proliferation of Alexander cells was inhibited with P-10.2 or PS but not with IL-1. P-10.2 inhibited the growth of MH134 at day 1 and 3, while the growth of MH134 was shown not to be inhibited with PS and IL-1 at day 1, but rather suppressed them at day 3. These results suggest that P-10.2 augments the growth of non-malignant liver cells (Chang liver cells) but inhibits that of hepatoma cells (Alexander cells and MH134). P-10.2 enhanced fibrinogen and hepatoglobin secretion from Chang liver cells. In addition to their liver cell activation, P-10.2 and PS stimulated ACTH and beta-endorphin secretion from AtT-20 cells.
Ishizaka
S
S
Third Department of Internal Medicine, Nara Medical University, Japan.
Tsujii
T
T
D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006528:Carcinoma, Hepatocellular; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005340:Fibrinogen / Q000096:biosynthesis; D005914:Globins / Q000096:biosynthesis; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008113:Liver Neoplasms; D008114:Liver Neoplasms, Experimental; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D010911:Pituitary Neoplasms; D012471:Salivary Proteins and Peptides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D001615:beta-Endorphin / Q000096:biosynthesis
D007375:Interleukin-1; D046911:Macromolecular Substances; D010446:Peptide Fragments; D012471:Salivary Proteins and Peptides; C067666:hepatoglobin; D001615:beta-Endorphin; D005340:Fibrinogen; D000324:Adrenocorticotropic Hormone; D005914:Globins