[Study on the effect of monoclonal antibodies on Giardia lamblia in vitro].
Monoclonal antibodies (McAbs) were generated by fusion of SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with Giardia lamblia trophozoites. To define the effect of the McAb on Giardia lamblia, trophozoites were subcultured axenically after contact with McAb for 1 h at 37 degrees C. The McAbs 1G5 and 1E2 (both IgG1) were able to inhibit the growth of trophozoite at the rate of 63.4% and 35%, respectively. The inhibitory effect was decreased along with the decreasing quantity of McAb (from dilution 1:45 to 1:225) added to the TYI-S-33 medium. The actions of McAbs could be eliminated by absorption with trophozoites and not be influenced by complement in fresh guinea-pig serum, thus the actions of McAbs appeared to be specific and complement independent.
Jia
K D
KD
Shandong Institute of Parasitic Diseases, Jining.
Zhu
Y G
YG
Lu
S Q
SQ
Wang
F R
FR
Li
G P
GP
Wen
P E
PE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000494:pharmacology*; D016829:Giardia lamblia / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal