Trolox protects hyperglycemia-induced cataractogenesis in cultured rat lens.
Hyperglycemia-induced cataractogenesis has been studied in rat lenses cultured in 50 mM glucose using an inverted microscope connected with a Universal C-mount and a CCD camera. Digital images were acquired and the opacity was determined by quantitating the transmitted light. Antioxidants, butylated hydroxy toluene (BHT) and 6-hydroxy-2,5,7,8-tetramethenyl-chroman-2-carboxylic acid (Trolox) provided good protection against 50 mM glucose-induced cataractogenesis in rat lenses for upto 8 days. Sorbitol levels in the 50 mM glucose+antioxidant groups were approximately 1.5 mM fold higher than in 50 mM glucose. The results, besides further demonstrating that oxidative damage is the major mechanism of sugar-induced cataractogenesis, show that Trolox or related amphipathic compounds could be of therapeutic use in the prevention of diabetic cataracts.
Ansari
N H
NH
Department of Ophthalmology, University of Texas Medical Branch, Galveston 77555.
Bhatnagar
A
A
Fulep
E
E
Khanna
P
P
Srivastava
S K
SK
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002084:Butylated Hydroxytoluene / Q000627:therapeutic use; D002386:Cataract / Q000209:etiology* / Q000517:prevention & control*; D002478:Cells, Cultured; D002839:Chromans / Q000627:therapeutic use*; D004347:Drug Interactions; D006943:Hyperglycemia / Q000150:complications*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013012:Sorbitol / Q000494:pharmacology
D000975:Antioxidants; D002839:Chromans; D002084:Butylated Hydroxytoluene; D013012:Sorbitol; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid