title: Neurotrophins affect survival and neuritogenesis by adult injured auditory neurons in vitro.
abstract: This study evaluates the trophic effects of three neurotrophins on traumatized adult auditory neurons in culture, and the presence of these neurotrophins in cochlear nucleus tissue. BDNF and NT-3 promoted survival but very limited neuritogenesis by adult auditory neurons in vitro, while NGF, although without a survival effect, evoked a robust neuritic outgrowth response when combined with BDNF. Messenger RNAs that encode for NGF, BDNF and NT-3 were detected by RT-PCR in RNA extracts from adult cochlear nuclei tissue. Based on these in vitro and in vivo findings, we propose NT-3 as the agent of the peripheral target-derived survival promoting effect and NGF, BDNF, and NT-3 as mediators of trophic influences originating from the central target (i.e. cochlear nucleus).
authors.affiliation: Department of Otorhinolaryngology and Audiophonology, University of Liege, Belgium.
authors.forename: P P
authors.initials: PP
authors.lastname: Lefebvre
authors.forename: B
authors.initials: B
authors.lastname: Malgrange
authors.forename: H
authors.initials: H
authors.lastname: Staecker
authors.forename: M
authors.initials: M
authors.lastname: Moghadass
authors.forename: T R
authors.initials: TR
authors.lastname: Van de Water
authors.forename: G
authors.initials: G
authors.lastname: Moonen
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017626:Cochlear Nucleus / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000187:drug effects* / Q000648:ultrastructure; D020933:Neurotrophin 3; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D000159:Vestibulocochlear Nerve / Q000166:cytology; D061285:Vestibulocochlear Nerve Injuries
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3