Comparative, quantitative study of lymphoid and non-lymphoid uptake of 60 nm polystyrene particles.
Uptake by gut epithelial tissue of 60 nm polystyrene particles was studied in female Sprague-Dawley rats (180 g, 9 weeks old) after 5 days oral dosing by gavage (14 mg/kg). The gut was divided into lymphoid and non-lymphoid tissue of the small and large intestine, prior to analysis for polystyrene by gel permeation chromatography (GPC). Approximately 10% of the administered dose was recovered from the entire gastrointestinal tract. The total percentage of the administered dose taken up through lymphoid tissue was statistically much greater than through non-lymphoid tissue. It was estimated that 60% of the uptake in the small intestine occurred through the Peyer's patches, even though the patches comprised a small percentage of the total surface area of the small intestinal tissue. A significant amount of the total uptake was shown to occur in the large intestine, particularly in the lymphoid sections of this tissue. These results were confirmed by fluorescence microscopy.
Hillery
A M
AM
Centre for Drug Delivery Research, School of Pharmacy, University of London, UK.
Jani
P U
PU
Florence
A T
AT
D000818:Animals; D002850:Chromatography, Gel; D005260:Female; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008221:Lymphoid Tissue / Q000166:cytology / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008863:Microspheres; D010581:Peyer's Patches / Q000378:metabolism; D011137:Polystyrenes; D051381:Rats; D017207:Rats, Sprague-Dawley
D011137:Polystyrenes