title: A comparison of human and bovine Escherichia coli O157:H7 isolates by toxin genotype, plasmid profile, and bacteriophage lambda-restriction fragment length polymorphism profile.
abstract: Foods of bovine origin have been linked to human disease outbreaks caused by Escherichia coli O157:H7 and may be linked to the more common sporadic cases as well. In this study, E. coli O157:H7 from the bovine reservoir (22 isolates: 12 from dairy and 10 from beef breed cows) and from human patients (50 isolates from sporadic human infections) were compared using Shiga-like toxin genotypes, plasmid profiles, and DNA restriction fragment length polymorphisms identified with a bacteriophage lambda probe (lambda-RFLP). Twenty-three lambda-RFLP profiles, 4 Shiga-like toxin genotypes, and 8 plasmid profiles were identified among the isolates tested. Together the typing methods distinguished 43 strains, of which 3 were isolated from both humans (5 isolates) and cattle (6 isolates; 5 from dairy herds). These data demonstrate the value of lambda-RFLP as a means of strain identification for E. coli O157:H7.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University College of Veterinary Medicine, Pullman.
authors.forename: M
authors.initials: M
authors.lastname: Paros
authors.forename: P I
authors.initials: PI
authors.lastname: Tarr
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: T E
authors.initials: TE
authors.lastname: Besser
authors.forename: D D
authors.initials: DD
authors.lastname: Hancock
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D002417:Cattle; D004197:Disease Reservoirs; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2
chemical_list: D001427:Bacterial Toxins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2