Identification of noradrenaline in venom from the funnel-web spider Hololena curta.
Venom from the funnel-web spider Hololena curta was added to the Krebs-Henseleit solution bathing isolated ring preparations of rat thoracic aorta, suspended in water-jacketed organ baths, for the purpose of tension recordings. Hololena curta venom at dilutions of 1:100,000 to 1:1000 caused a marked vasoconstriction, which was completely inhibited by the alpha 1 adrenoceptor antagonist prazosin (1 microm). The vasoconstriction appears to be due to the direct effects on alpha 1 adrenoceptors of a venom constituent, which we have identified using HPLC/ECD as the catecholamine, noradrenaline.
Frew
R
R
Pharmacology and Therapeutics, Defense Research Establishment Suffield, Alberta, Canada.
Hamilton
M G
MG
Lundy
P M
PM
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000032:analysis / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009638:Norepinephrine / Q000032:analysis / Q000494:pharmacology*; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000032:analysis / Q000494:pharmacology; D013111:Spider Venoms / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology*; D014661:Vasoconstriction / Q000187:drug effects*
D013111:Spider Venoms; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine; D011224:Prazosin