title: Unique change of pancreastatin-like immunoreactivity in cerebrospinal fluid by aging.
abstract: Using a specific antiserum for the C-terminal glycine amide region of human pancreastatin (PST), pancreastatin-like immunoreactivity (PST-LI) was measured in cerebrospinal fluid (CSF) from 447 subjects (368 +/- 10.8 pmol/l, mean +/- S.E.M.) free from endocrine diseases. The CSF contents of PST-LI showed a mountain-shape type change which peaked at 40 years of age. The highest concentration was found in the group of ages 40-49 years old (412 +/- 22.9 pmol/l) and the lowest concentration was found in the group of ages 80-89 years old (293.2 +/- 45.2 pmol/l) among various age groups. Gel chromatographic examination revealed the presence of two major forms (MW 13,500 and 5,400) of PST-LI in CSF. Because of the character of this antibody, the large molecular form is possibly an N-terminally elongated PST and the other may be PST-52. This may be the first report on the unique age-related change of PST concentration in CSF.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sekiya
authors.forename: R
authors.initials: R
authors.lastname: Nakao
authors.forename: R
authors.initials: R
authors.lastname: Takayanagi
authors.forename: M
authors.initials: M
authors.lastname: Haji
authors.forename: S
authors.initials: S
authors.lastname: Kurose
authors.forename: M
authors.initials: M
authors.lastname: Oyama
authors.forename: M
authors.initials: M
authors.lastname: Ohashi
authors.forename: K
authors.initials: K
authors.lastname: Tateishi
authors.forename: A
authors.initials: A
authors.lastname: Funakoshi
authors.forename: K
authors.initials: K
authors.lastname: Akazawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000134:cerebrospinal fluid*; D002850:Chromatography, Gel; D053379:Chromogranin A; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010187:Pancreatic Hormones / Q000134:cerebrospinal fluid* / Q000737:chemistry / Q000276:immunology; D011863:Radioimmunoassay
chemical_list: D053379:Chromogranin A; D010187:Pancreatic Hormones; C050836:pancreastatin