title: Crystallization and preliminary X-ray diffraction studies of xylose isomerase from Thermoanaerobacterium thermosulfurigenes strain 4B.
abstract: Xylose isomerase from the thermophile Thermoanaerobacterium thermosulfurigenes strain 4B has been crystallized by vapour diffusion from Jeffamine ED 4000 as precipitant. The crystal symmetry is P2(1)2(1)2(1), with unit cell dimensions a = 85.6 A, b = 153.5 A and c = 158.5 A. The protein molecular mass and volume of the unit cell is consistent with the presence of a tetramer of the enzyme in the asymmetric unit. The crystals diffract X-rays from a synchrotron source to 1.7 A resolution.
authors.affiliation: Blackett Laboratory, Imperial College of Science, Technology and Medicine, London, U.K.
authors.forename: L F
authors.initials: LF
authors.lastname: Lloyd
authors.forename: O S
authors.initials: OS
authors.lastname: Gallay
authors.forename: J
authors.initials: J
authors.lastname: Akins
authors.forename: J G
authors.initials: JG
authors.lastname: Zeikus
mesh_terms: D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006093:Gram-Positive Asporogenous Rods / Q000201:enzymology*
chemical_list: D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase