Comparison of monoclonal antibody-based sandwich enzyme-linked immunosorbent assay and virus isolation for detection of peste des petits ruminants virus in goat tissues and secretions.
A monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (S-ELISA) was developed for specific detection of peste des petits ruminants virus. Compared with virus isolation in Vero cell cultures using 89 paired tissue and secretion samples from six experimentally infected goats, S-ELISA was significantly more sensitive (71.9% versus 65.2%; P < 0.05). The S-ELISA is a suitable alternative to virus isolation.
Saliki
J T
JT
Foreign Animal Disease Diagnostic Laboratory, U.S. Department of Agriculture, Plum Island, Greenport, New York 11944.
House
J A
JA
Mebus
C A
CA
Dubovi
E J
EJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000302:isolation & purification; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D015511:Goat Diseases / Q000175:diagnosis* / Q000382:microbiology; D006041:Goats; D018185:Morbillivirus Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D018107:Peste-des-petits-ruminants virus / Q000276:immunology* / Q000302:isolation & purification*; D012301:Rinderpest / Q000175:diagnosis; D012680:Sensitivity and Specificity; D014709:Vero Cells; D014773:Virology / Q000379:methods*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral