title: The leucine-zipper in elongation factor EF-1 delta, a guanine-nucleotide exchange protein, is conserved in Artemia and Xenopus.
abstract: Elongation factor 1, a complex involved in protein biosynthesis, contains two guanine-nucleotide-exchange proteins EF-1 beta and EF-1 delta. The sequence of EF-1 delta of Artemia was determined with the purified protein. When compared to EF-1 delta from Xenopus, a high degree of identify (80%) was found in the C-terminal domains of the proteins, which contain the guanine-nucleotide-exchange activity. The N-terminal domains share only 23% of the amino acids at identical positions, and therefore they were further analysed for less obvious types of homology. To this end, a published approach for sequence analysis, which can detect peculiar amino acid patterns in proteins was applied. In this way, a weak albeit unmistakable similarity between the two EF-1 delta proteins was demonstrated in the region of the leucine-zippers, apart from the leucine repeat itself. Apparently, they display a common structural pattern in their N-terminal domains, which so far has been observed mainly in transcription factors.
authors.affiliation: Department of Medical Biochemistry, Sylvius Laboratories, Leiden, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Amons
authors.forename: M A
authors.initials: MA
authors.lastname: Guerrucci
authors.forename: R H
authors.initials: RH
authors.lastname: Karssies
authors.forename: J
authors.initials: J
authors.lastname: Morales
authors.forename: P
authors.initials: P
authors.lastname: Cormier
authors.forename: W
authors.initials: W
authors.lastname: Möller
authors.forename: R
authors.initials: R
authors.lastname: Bellé
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000235:genetics*; D016384:Consensus Sequence; D017124:Conserved Sequence; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000235:genetics*; D012261:Ribonucleoproteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014981:Xenopus / Q000235:genetics*
chemical_list: D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012261:Ribonucleoproteins; D019204:GTP-Binding Proteins