A plant scaffold attached region detected close to a T-DNA integration site is active in mammalian cells.
Integration of foreign genes into plant genomes by the Agrobacterium T-DNA transfer system has been considered to occur at random. It has been speculated that the chromosomal structure of the integration site might affect the expression pattern of the introduced genes. To gain insight into the molecular structure of T-DNA integration sites and its possible impact on gene expression, we have examined plant DNA sequences in the vicinity of T-DNA borders. Analysis of a transgenic petunia plant containing a chloramphenicol acetyltransferase (CAT) gene regulated by the hemoglobin promoter (PAR) from Parasponia andersonii revealed a scaffold attachment region (SAR) close to one T-DNA end. In addition to having strong binding affinities for both animal and plant nuclear scaffolds this petunia SAR element is as active in mammalian cells as the authentic elements from mammalian sources.
Dietz
A
A
Institut für Biochemie und Pflanzenvirologie, Biologische Bundesanstalt, Braunschweig, Germany.
Kay
V
V
Schlake
T
T
Landsmann
J
J
Bode
J
J
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli; D007887:Fabaceae / Q000235:genetics; D018014:Gene Transfer Techniques; D016678:Genome; D006454:Hemoglobins / Q000235:genetics; D007739:L Cells; D008156:Luciferases / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014170:Transformation, Genetic; D016662:Virus Integration
D004269:DNA, Bacterial; D006454:Hemoglobins; C036483:T-DNA; D004247:DNA; D008156:Luciferases