Chromosome localization and orientation of the simple sequence repeat of human satellite I DNA.
The predominant chromosomal locations of human satellite I DNA were detected using fluorescent in situ hybridization (FISH). Synthetic deoxyoligonucleotides designed from consensus sequences of the simple sequence repeats of satellite 1 were used as probes. The most abundant satellite I repeat, the -A-B-A-B-A- form, is located at the pericentromeric regions of chromosomes 3, 4, 13, 14, 15, 21, and 22. The less abundant -B-B-B-form was not detected on chromosome 4, but was present at all the other locations. A variation of FISH that allows strand-specific hybridization of single-stranded probes (CO-FISH) determined that the human satellite I sequences are predominantly arranged in head-to-tail fashion along the DNA strand.
Meyne
J
J
Life Sciences Division, Los Alamos National Laboratory, NM 87545.
Goodwin
E H
EH
Moyzis
R K
RK
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D016384:Consensus Sequence / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008969:Molecular Sequence Data; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D004276:DNA, Satellite; D011089:Polydeoxyribonucleotides