title: Absolute bioavailability of lead acetate and mining waste lead in rats.
abstract: The primary purpose of this study was to generate data that could be used to determine the absolute bioavailability of lead using data from a previous study in which soil containing lead from mining waste was mixed with feed. Young male and female Sprague-Dawley rats (7-8 weeks of age, five/sex/group) were given either soluble lead acetate mixed in a purified diet (AIN-76) at three different dose levels (1, 25, and 250 ppm Pb for 30 consecutive days) or intravenously at doses of 0.02, 0.20, and 2.0 mg Pb/kg BW for 29 days. A control group (purified diet only) was also included. The intravenous groups were used to provide maximal absorption (lead presumed to be 100% bioavailable) and accumulation data for lead in blood, bone, and liver. The lead acetate groups were used to evaluate the comparability of the present study with a previous study that compared bioavailable lead from ingested soil and lead acetate. Group mean whole blood, bone and liver lead concentration values increased with increasing dose levels for all treatment groups. A linear relationship was observed between blood lead concentration and dose following intravenous administration of lead and this provided empirical support for using blood lead concentrations at supposed steady state (approximately 30 days) to compute the bioavailability of lead administered by different routes and from different sources. The absolute bioavailability values of mining waste lead in soil were low based on the results for all tissue types. Absolute bioavailability values for lead acetate in dosed feed for blood, bone, and liver were approximately 6-, 19-, and 20-fold greater, respectively, than mining waste lead. Based on the current design and test system used, the absolute bioavailability of mining waste lead in soil administered in feed was approximately 3% based on blood data and less than 1% based on bone and liver data. These data are consistent with the low solubility of the constituent lead mineral phases in Butte soils.
authors.affiliation: Battelle, Columbus, Ohio 43201.
authors.forename: G B
authors.initials: GB
authors.lastname: Freeman
authors.forename: J D
authors.initials: JD
authors.lastname: Johnson
authors.forename: S C
authors.initials: SC
authors.lastname: Liao
authors.forename: P I
authors.initials: PI
authors.lastname: Feder
authors.forename: A O
authors.initials: AO
authors.lastname: Davis
authors.forename: M V
authors.initials: MV
authors.lastname: Ruby
authors.forename: R A
authors.initials: RA
authors.lastname: Schoof
authors.forename: R L
authors.initials: RL
authors.lastname: Chaney
authors.forename: P D
authors.initials: PD
authors.lastname: Bergstrom
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D001842:Bone and Bones / Q000378:metabolism; D005260:Female; D007220:Industrial Waste; D007275:Injections, Intravenous; D007854:Lead / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism; D008297:Male; D008906:Mining; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012989:Soil Pollutants / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D007220:Industrial Waste; D009942:Organometallic Compounds; D012989:Soil Pollutants; D007854:Lead; C008261:lead acetate