Isolation of an adhesin from Staphylococcus aureus that binds Lewis a blood group antigen and its relevance to sudden infant death syndrome.
A 67 kDa protein was isolated from cell membrane preparations of Staphylococcus aureus (NCTC 10655) by affinity adsorption with synthetic Lewis a antigen conjugated to Synsorb beads. Pre-treatment of buccal epithelial cells expressing Lewis a with the purified protein reduced binding of the staphylococcal strain to a greater extent than the material not bound to the Synsorb beads. The significance of this work is discussed with reference to expression of Lewis a antigen in infants and the proposed role of toxigenic strains of staphylococci in some cases of sudden infant death syndrome.
Saadi
A T
AT
Department of Medical Microbiology, University of Edinburgh, Medical School, UK.
Weir
D M
DM
Poxton
I R
IR
Stewart
J
J
Essery
S D
SD
Blackwell
C C
CC
Raza
M W
MW
Busuttil
A
A
D018829:Adhesins, Bacterial; D001422:Bacterial Adhesion / Q000276:immunology* / Q000502:physiology*; D001667:Binding, Competitive; D002473:Cell Wall / Q000737:chemistry; D002610:Cheek; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007223:Infant; D007983:Lewis Blood Group Antigens / Q000378:metabolism*; D009061:Mouth Mucosa / Q000166:cytology; D013211:Staphylococcus aureus / Q000276:immunology*; D013398:Sudden Infant Death / Q000209:etiology
D018829:Adhesins, Bacterial; D007983:Lewis Blood Group Antigens; C048165:adhesin, Staphylococcus aureus