Rapid, nonradioactive, and quantitative method to analyze zona pellucida modifications in single mouse eggs.
A rapid, nonradioactive method to monitor the ZP2 to ZP2f conversion in the zona pellucida of single mouse eggs has been developed. This assay is based on the chemiluminescent detection of biotinylated ZP2 and ZP2f following electrophoresis under reducing conditions and electrophoretic transfer to Immobilon P. This method is about 10 times faster and detects similar extents of ZP2 to ZP2f conversion following A23187-induced egg activation, when compared to the commonly used radioiodination procedures.
Moos
J
J
Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104-6018.
Kalab
P
P
Kopf
G S
GS
Schultz
R M
RM
D000818:Animals; D001345:Autoradiography; D001710:Biotin; D015153:Blotting, Western; D004527:Egg Proteins / Q000032:analysis* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005069:Evaluation Studies as Topic; D005260:Female; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008163:Luminescent Measurements; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000378:metabolism; D008722:Methods; D051379:Mice; D011956:Receptors, Cell Surface; D015044:Zona Pellucida / Q000737:chemistry* / Q000378:metabolism; D000072216:Zona Pellucida Glycoproteins
D004527:Egg Proteins; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; C000606520:Zp2 protein, mouse; D001710:Biotin