The chemiluminescence assay of lipid peroxidation products in human blood plasma lipoproteins.
A chemiluminescence (CL) flash kinetics on the addition of Fe2+ ions into oxidized low density lipoprotein (LDL) suspension has been studied. LDL oxidation was carried out at 37 degrees C without and in the presence of 5 or 50 microM of Cu2+. It has been found that under certain experimental conditions (the addition of excess iron ions, more than 1 mM) the amplitude of CL flash depended almost linearly (1) on the concentration of oxidized LDL and (2) on the extent of LDL oxidation measured as diene conjugates (DC) and 2-thiobarbituric acid-reactive substance (TBARS) accumulation. The corresponding correlation coefficients were: for TBARS -0.94 and for DC -0.97, in the case of LDL autooxidation; 0.72 and 0.98, in the case of copper-induced LDL oxidation. A sensitivity of the CL method was shown to be significantly enhanced (by more than two orders) in the presence of CL sensitizer--2,3,5,6-1H,4H-tetrahydro-9-(2'-benzoimidazolyl)-qui nolizin-(9,9a,1- gh)coumarin.
Driomina
E
E
Institute for Physical-Chemical Medicine, Moscow, Russia.
Polnikov
I
I
Sharov
V
V
Azizova
O
O
Vladimirov
Y
Y
D004396:Coloring Agents; D003374:Coumarins; D000431:Ethanol; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation; D008074:Lipoproteins / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood; D008163:Luminescent Measurements; D013053:Spectrophotometry; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D004396:Coloring Agents; D003374:Coumarins; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol; C030123:coumarin