title: An adaptation of the Maxam and Gilbert sequencing method to the study of human metaphase chromosomes.
abstract: We have adapted the chemical DNA sequencing method developed by Maxam and Gilbert to study human metaphase chromosomes. Particularly, the in situ cytosine-specific chemical cleavage has been assayed. After exhaustive treatments, a C-like banding pattern was consistently shown. Since methyl groups interfere with the cytosine-specific chemical cleavage reaction, we assume that heterochromatin remains resistant as a result of the high level of methylation. On the other hand when moderate treatments are applied, T-bands remain as resistant regions, implying that these domains would be the most intensely methylated regions within euchromatin.
authors.affiliation: Dpto. de Biologia, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, Spain.
authors.forename: S E
authors.initials: SE
authors.lastname: De Cabo
authors.forename: G
authors.initials: G
authors.lastname: Visedo
authors.forename: J
authors.initials: J
authors.lastname: Fernández-Piqueras
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D003582:Cytogenetics / Q000379:methods; D004247:DNA / Q000737:chemistry*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000648:ultrastructure; D008677:Metaphase
chemical_list: D004247:DNA