Ascorbic acid reduces the endotoxin-induced lung injury in awake sheep.
Our aim was to investigate whether ascorbic acid can reduce reactive oxygen metabolite-mediated acute lung injury. The effects of intravenous administration of Escherichia coli endotoxin were studied, with and without ascorbic acid infusion, on haemodynamics, lung lymph flow, cardio-respiratory and neutrophil function in chronically instrumented sheep. Paired experiments were performed on eight sheep in which they received either endotoxin alone (0.5 micrograms kg-1 b.w.) (ET group) or in combination with an ascorbic acid infusion (1 g kg-1 b.w. bolus injection followed by 0.2 g kg-1 h-1 continuous infusion) ET + ASC group) in random order. Four of the animals also received ascorbic acid alone (ASC group). As a result, for the ET + ASC group a general and mostly significant improvement (P < 0.05) in the early hypertensive phase (0-60 min, P values) and in the late permeability phase (2-4 h, *P values) of cardiorespiratory function (mean artery pressure: P/*P = 0.283/0.049; mean pulmonary artery pressure: P/*P = 0.0001/0.0001; mean pulmonary artery wedge pressure: P/*P = 0.012/0.001; right ventricular stroke work index: P/*P = 0.02/0.0001; cardiac index: P/*P = 0.797/0.755; arterial oxygen saturation: P/*P = 0.0059/0.01; arterial-venous difference of oxygen tension: P/*P = 0.011/0.0005), oxygen consumption: P/*P = 0.013/0.035, lung lymph flow: P/*P = 0.562/0.012, lymph/plasma protein ratio: P/*P = 0.304/0.008 and protein clearance: P/*P = 0.56/0.05 was observed in comparison with the ET group.(ABSTRACT TRUNCATED AT 250 WORDS)
Dwenger
A
A
Institut für Klinische Biochemie, Medizinische Hochschule Hannover, Germany.
Pape
H C
HC
Bantel
C
C
Schweitzer
G
G
Krumm
K
K
Grotz
M
M
Lueken
B
B
Funck
M
M
Regel
G
G
D000818:Animals; D001205:Ascorbic Acid / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D006439:Hemodynamics / Q000187:drug effects; D007958:Leukocyte Count / Q000187:drug effects; D008208:Lymphatic System / Q000187:drug effects / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000517:prevention & control*; D012756:Sheep
D004731:Endotoxins; D017382:Reactive Oxygen Species; C044101:endotoxin, Escherichia coli; D001205:Ascorbic Acid