Analysis of T cell receptor variability in fresh tumor-infiltrating lymphocytes from human head and neck cancer.
In this study, we analyzed T cell receptor (TCR) gene rearrangements in tumor-infiltrating lymphocytes (TIL) freshly obtained from 15 patients with head and neck cancer using the reversely transcribed polymerase chain reaction (RT-PCR) method. These TILs showed preferential expression of V alpha 10, V alpha 8 and V alpha 1, detected in 13 (87%), 11 (73%), and 9 cases (60%), respectively. The TCRV beta gene revealed diversity without preferential usage. The head and neck region is exposed to bacteria and viruses, so it is possible that the tumor site can become infected and accumulate T cells involved in infection and inflammation. Therefore, we also investigated TCR gene usage in T cells infiltrating in chronic sinusitis mucosa to address the question of whether the V alpha 1, V alpha 8, and V alpha 10 subfamilies are characteristic in TIL from squamous cell carcinoma of head and neck. TCR V alpha 10 gene usage was also the most common in V alpha segment in T cells infiltrating the sinus mucosa, but V alpha 1 and V alpha 8 were not detected in the T cells in sinusitis. These results indicate that the V alpha 10 subfamily, the preferred T cell population in both TIL and T cells in inflammatory disease, might be involved mainly in inflammation or infection. On the other hand, V alpha 1 and V alpha 8 appear to be relatively specific populations for antitumor immunity in head and neck cancer.
Chikamatsu
K
K
Department of Otolaryngology, Kumamoto University, School of Medicine.
Eura
M
M
Nakano
K
K
Kanzaki
Y
Y
Matsuoka
H
H
Masuyama
K
K
Ishikawa
T
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002470:Cell Survival / Q000502:physiology; D002908:Chronic Disease; D005260:Female; D015870:Gene Expression; D015329:Gene Rearrangement, T-Lymphocyte; D014644:Genetic Variation; D006258:Head and Neck Neoplasms / Q000276:immunology / Q000473:pathology*; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000502:physiology* / Q000648:ultrastructure*; D008297:Male; D008443:Maxillary Sinus / Q000473:pathology; D015523:Maxillary Sinusitis / Q000276:immunology / Q000473:pathology; D008875:Middle Aged; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000473:pathology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000502:physiology
D011948:Receptors, Antigen, T-Cell