title: Effects of juvenile hormone on mammalian steroidogenesis.
abstract: The effects of juvenile hormone-III (JH-III) and the JH analogue 2-(4-phenoxyphenoxy)-ethoxyte-trahydropiran on testicular steroidogenesis were studied. By using cultured MA-10 Leydig tumor cells as a model, these compounds were found to be potent inhibitors of LH/hCG steroidogenic action in a dose-dependent manner. Scatchard plot analysis of the binding data indicated that the JH analogue did not significantly alter the affinity nor the number of hCG binding sites, as well as GTP binding to plasma membranes. JH analogue inhibited the stimulatory action of both cholera toxin and forskolin on cAMP production and the concomitant steroidogenic response. JH analogue inhibited (Bu)2cAMP-stimulated progesterone synthesis, indicating that a process downstream to the adenylyl cyclase in the steroidogenic pathway is also affected.
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME)-CONICET, Buenos Aires, Argentina.
authors.forename: E A
authors.initials: EA
authors.lastname: Vladusic
authors.forename: L E
authors.initials: LE
authors.lastname: Bussmann
authors.forename: P E
authors.initials: PE
authors.lastname: Visconti
authors.forename: A M
authors.initials: AM
authors.lastname: Stoka
authors.forename: J B
authors.initials: JB
authors.lastname: Rodriguez
authors.forename: E G
authors.initials: EG
authors.lastname: Gros
authors.forename: E H
authors.initials: EH
authors.lastname: Charreau
mesh_terms: D000818:Animals; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000378:metabolism / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D007605:Juvenile Hormones / Q000494:pharmacology*; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D008322:Mammals; D011374:Progesterone / Q000096:biosynthesis*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D006063:Chorionic Gonadotropin; D007605:Juvenile Hormones; D011374:Progesterone; D006160:Guanosine Triphosphate; D002772:Cholera Toxin; D000242:Cyclic AMP