Versatile Escherichia coli expression vectors for production of truncated proteins.
Several expression vector plasmids containing the tac promoter, the rrnBT1T2 terminator, and the pUC ori sequence were constructed. Some of them, the pES series, have a start codon in all three reading frames and multiple cloning sites downstream of the tac promoter and have stop codons also in all three frames and additional stop codons accompanying restriction sites. They are designed for versatile expression of truncated proteins which are produced by deleting portions of the inserted DNA.
Omori
K
K
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Akatsuka
H
H
Komatsubara
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D003062:Codon; D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004926:Escherichia coli; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D016364:Reading Frames; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015183:Restriction Mapping; D017384:Sequence Deletion; D013379:Substrate Specificity; D013728:Terminator Regions, Genetic
D003062:Codon; D011994:Recombinant Proteins; D004262:DNA Restriction Enzymes