3-Aminobenzamide induces cytoskeleton rearrangement in M14 melanoma cells.
3-aminobenzamide (3-ABA) is an inhibitor of poly-ADP-ribose-polymerase, an enzyme involved in numerous subcellular processes including cell death. With the aim of contributing to clarify the mode of action of the drug, which is still poorly understood, its effects on cultured melanoma cells M14 have been assessed. In particular, an impairment of cell growth accompanied by the formation of long and numerous dendritic-like protrusions has been detected. This finding appears to be due to a specific effect of the drug on some cytoskeletal elements and could be associated with its differentiating capability. This finding, together with previous data from our group--Biochem. Biophys. Res. Commun., (1994) 199, 525-530 and 1250-1255--suggests that cytoskeleton is an important target of 3-ABA.
Malorni
W
W
Department of Ultrastructures, Istituto Superiore di Sanità, Roma, Italy.
Rainaldi
G
G
Straface
E
E
Rivabene
R
R
Cossarizza
A
A
Capri
M
M
Monti
D
D
Franceschi
C
C
D001549:Benzamides / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D008545:Melanoma / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D014407:Tumor Cells, Cultured
D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide