title: Metalloporphyrin speciation by liquid chromatography and inductively coupled plasma-mass spectrometry.
abstract: Inductively coupled plasma-mass spectrometry is investigated for the detection of metalloporphyrins (cobalt protoporphyrin, hemin, and zinc protoporphyrin) separated by liquid chromatography. A Hypersil SAS C1 column with mobile phase containing 68% methanol at a pH of 4.5 is used. The detection limits obtained are in the nanogram range for cobalt and zinc protoporphyrins, whereas for hemin, detection limits are in the microgram range. The accuracy of the method is evaluated by determining zinc protoporphyrin from the whole blood of a lead-poisoned patient.
authors.affiliation: Department of Chemistry, University of Cincinnati, Ohio 45221-0172.
authors.forename: U
authors.initials: U
authors.lastname: Kumar
authors.forename: J G
authors.initials: JG
authors.lastname: Dorsey
authors.forename: J A
authors.initials: JA
authors.lastname: Caruso
authors.forename: E H
authors.initials: EH
authors.lastname: Evans
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003035:Cobalt / Q000097:blood; D006801:Humans; D007501:Iron / Q000097:blood; D007855:Lead Poisoning / Q000097:blood; D013058:Mass Spectrometry / Q000379:methods*; D009330:Nebulizers and Vaporizers; D011524:Protoporphyrins / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D011524:Protoporphyrins; C017803:zinc protoporphyrin; D003035:Cobalt; D007501:Iron