[Direct radioimmunoassay of the dehydroepiandrosterone sulfate level in blood plasma and its use for evaluation of the androgenic function of the adrenal cortex].
The author suggests a direct radioimmunoassay of dehydroepiandrosterone sulfate (DEA-S) in 0.05-2 microliters of human blood plasma, making use of an antiserum prepared by rabbit immunization with DEA-S hemisuccinate conjugate with bovine serum albumin and [7-3H]-DEA. The method is highly sensitive (0.039 mumol/l), specific, reliable, and easily reproducible. This method was used to measure plasma DEA-S concentrations in healthy subjects of various ages, in women in different phases of the cycle, after dexamethasone load, and in patients with the Stein-Loewenthal's syndrome and the adrenogenital syndrome. It is recommended for the diagnosis of retinopathies connected with adrenal and gonadal androgenic dysfunction.
Goncharova
N D
ND
D000302:Adrenal Cortex / Q000502:physiology*; D000728:Androgens / Q000502:physiology*; D000918:Antibody Specificity; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000097:blood; D019314:Dehydroepiandrosterone Sulfate; D003907:Dexamethasone; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D006868:Hydrolysis; D016014:Linear Models; D008297:Male; D011085:Polycystic Ovary Syndrome / Q000097:blood; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000728:Androgens; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D003907:Dexamethasone
Priamoĭ radioimmunologicheskiĭ analiz urovnia degidroépiandrosterona sulfata v plazme krovi i ego ispol'zovanie dlia otsenki androgennoĭ funktsii kory nadpochechnikov.