title: [The isolation and purification of porcine interferon-gamma].
abstract: Porcine immune interferon (IF-gamma) was generated by stimulation with bacterial lectins. Physicochemical analysis showed porcine IF-gamma to be a glycoprotein with an isoelectric point around 8.4-8.8 and an apparent molecular weight of 28000 to 30000. A purification process used in this work included sequential steps of precipitation with ammonium sulphate and chromatographic separation on GPG-glass, con A-Sepharose and biogel P-100. This purification process gave a preparation of IF-gamma with specific activity of around 7 x 10(4) units per mg of protein.
authors.forename: A V
authors.initials: AV
authors.lastname: Karpov
authors.forename: V K
authors.initials: VK
authors.lastname: Ivanenko
authors.forename: Ia G
authors.initials: IaG
authors.lastname: Kishko
authors.forename: N Ia
authors.initials: NIa
authors.lastname: Spivak
authors.forename: V D
authors.initials: VD
authors.lastname: Dumanskiĭ
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel; D007371:Interferon-gamma / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008722:Methods; D008970:Molecular Weight; D013552:Swine
chemical_list: D007371:Interferon-gamma
vernacular_title: Poluchenie i ochistka svinogo gamma-interferona.