Effect of fish oil and vitamin E on ethanol-induced changes in membrane ATPases.
We evaluated whether fish oil or vitamin E administration affected ethanol-induced changes in membrane ATPases. Male Wistar rats (225-250 g) were fed, through a gastric tube a liquid diet containing fish oil (25% of calories) and ethanol for one month. Another group of animals was given supplemental vitamin E (300 u/kg). In the pair-fed control animals, ethanol-derived calories were replaced with dextrose. The blood ethanol levels were maintained between 150 and 350 mg/dL. At sacrifice, the red cells were immediately washed with ice-cold saline, membranes were prepared and ATPases measured. These was no difference in the Na+K+ ATPase, Ca2+ ATPase and Mg2+ ATPase activities between the fish oil-dextrose and corn oil-dextrose groups. A decrease in Ca2+ ATPase and an increase in Na+K+ ATPase was seen with ethanol feeding; these change are similar to those seen in corn oil-ethanol fed rats. In contrast, Vitamin E administration prevented the ethanol-induced changes in ATPase. This observation provides support for the role of lipid peroxidation in alcohol-induced changes in cell membrane ATPase activities.
Nanji
A A
AA
Department of Pathology, New England Deaconess Hospital, Boston, MA 02215.
Sadrzadeh
S M
SM
D000251:Adenosine Triphosphatases / Q000097:blood* / Q000187:drug effects; D000818:Animals; D003314:Corn Oil / Q000494:pharmacology; D004032:Diet; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology*; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D005395:Fish Oils / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology*
D005395:Fish Oils; D014810:Vitamin E; C033266:Menhaden oil; D000431:Ethanol; D003314:Corn Oil; D000251:Adenosine Triphosphatases; D005947:Glucose