title: Multicenter evaluation of automated immunoturbidimetric assays for measurement of apolipoproteins A-I and B in serum and plasma.
abstract: Results of a multicenter evaluation of automated assays for measurement of apolipoproteins (apo) A-I and B with the Paramax analytical system are reported. Apo A-I and apo B response surface models were used to optimize concentrations of critical assay variables. Overall imprecision for apo A-I controls at concentrations of 1.01-1.61 g/L was 3.7-6.6%; overall imprecision for apo B controls at 1.00-1.61 g/L was 2.8-6.9%. There was no interference in apo A-I measurements. Albumin concentrations > 59 g/L resulted in a negative interference, and collection in sodium heparin caused a positive interference in apo B results. Apo A-I and apo B assays demonstrated acceptable agreement with comparative methods, although the Paramax apo B assays had a negative bias with respect to comparison methods. In 116 healthy individuals, serum apo A-I ranged from 0.97 to 2.05 g/L and serum apo B ranged from 0.51 to 1.32 g/L.
authors.affiliation: Department of Pathology, Loyola University Medical Center, Maywood, IL 60153.
authors.forename: S E
authors.initials: SE
authors.lastname: Kahn
authors.forename: R F
authors.initials: RF
authors.lastname: Labbé
authors.forename: E H
authors.initials: EH
authors.lastname: Pfadenhauer
authors.forename: A D
authors.initials: AD
authors.lastname: Kanonchoff
authors.forename: R T
authors.initials: RT
authors.lastname: Joseph
mesh_terms: D016632:Apolipoprotein A-I / Q000032:analysis*; D001055:Apolipoproteins B / Q000032:analysis*; D001331:Automation; D006801:Humans; D007118:Immunoassay; D007202:Indicators and Reagents; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D007202:Indicators and Reagents