Isolation of sulfonamides from fortified chicken tissues with supercritical CO2 and in-line adsorption.
Improved recoveries and detectability of three sulfonamides from chicken tissues by supercritical fluid extraction (SFE), without modifiers, using an in-line adsorption trap, are reported. Following SFE, the analytes are recovered from neutral alumina with the HPLC mobile phase. Samples are injected directly onto high-performance liquid chromatographic columns without post-extraction cleanup. Mean recoveries of sulfamethazine, sulfadimethoxine, and sulfaquinoxaline from liver, breast tissue, and thigh muscle are 89, 95, and 77%, respectively. The analytes are detectable at less than 100 ppb with a minimum of background interference.
Parks
O W
OW
U.S. Department of Agriculture, ARS, Eastern Regional Research Center, Philadelphia, Pennsylvania 19118.
Maxwell
R J
RJ
D000327:Adsorption; D000818:Animals; D002248:Carbon Monoxide; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005069:Evaluation Studies as Topic; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000032:analysis*; D013449:Sulfonamides / Q000302:isolation & purification*
D013449:Sulfonamides; D002248:Carbon Monoxide