Identification of the calcium-binding protein calgranulin in the matrix of struvite stones.
The identification of calcium-binding proteins in urine and kidney stones has led to a closer look at the role of matrix proteins in urolithiasis. We analyzed five struvite stones for protein content and identified two bands (8 and 14 KDa) that were confirmed by gel electrophoresis and amino acid sequencing to be calgranulin. This protein, which is known by several other names, has bacteriostatic antifungal activity. Its role in the formation of struvite stones warrants further investigation.
Bennett
J
J
Department of Urology, Massachusetts General Hospital, Boston.
Dretler
S P
SP
Selengut
J
J
Orme-Johnson
W H
WH
D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000302:isolation & purification*; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000302:isolation & purification*; D004586:Electrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007669:Kidney Calculi / Q000378:metabolism*; D039841:Leukocyte L1 Antigen Complex; D017616:Magnesium Compounds / Q000378:metabolism*; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism*; D000069877:Struvite
D002135:Calcium-Binding Proteins; D015816:Cell Adhesion Molecules, Neuronal; D039841:Leukocyte L1 Antigen Complex; D017616:Magnesium Compounds; D010710:Phosphates; D000069877:Struvite