title: Stoichiometry of the reaction catalyzed by skin sulfhydryl oxidase.
abstract: The stoichiometry of the reaction catalyzed by skin sulfhydryl oxidase was investigated. Dithiothreitol (DTT) was used as the substrate for skin sulfhydryl oxidase. The consumption of DTT, consumption of oxygen, and production of hydrogen peroxide were measured during the enzyme reaction. The molar ratio of DTT:O2:H2O2 in the enzyme reaction was 1:1.02:0.89. Correspondingly, the stoichiometry of the enzyme reaction was calculated to be [formula: see text]
authors.affiliation: Department of Dermatology, School of Medicine, Juntendo University, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Suga
authors.forename: K
authors.initials: K
authors.lastname: Takamori
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D004229:Dithiothreitol / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000201:enzymology*; D050862:Oxidoreductases Acting on Sulfur Group Donors
chemical_list: D004229:Dithiothreitol; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors