Nucleotide sequence and infectivity of cucumber mosaic cucumovirus (strain K) RNA2 involved in breakage of replicase-mediated resistance in tobacco.
Replicase-mediated resistance represents a genetically engineered form of resistance against viral infections and is established by using an intact or modified viral polymerase (replicase) gene for the transformation of the host. The K strain of cucumber mosaic cucumovirus (K-CMV) has been shown to break replicase-mediated resistance generated using a modified RNA2 of the subgroup I strain Fny-CMV. A full-length cDNA clone of K-CMV RNA2 was generated and RNA transcripts were shown to be infectious in combination with Fny-CMV RNAs 1 and 3. We further demonstrate that the ability of K-CMV to overcome resistance in the transgenic plants maps to RNA2. The complete nucleotide sequence of this RNA was determined and sequence differences from Fny-CMV RNA2 are discussed with regard to functional domains and their possible involvement in resistance breakage.
Hellwald
K H
KH
Department of Plant Pathology, Cornell University, Ithaca, New York 14853.
Palukaitis
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D017799:Cucumovirus / Q000235:genetics / Q000472:pathogenicity*; D005818:Genetic Engineering; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010947:Plants, Toxic; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000382:microbiology*; D014774:Virulence / Q000235:genetics
D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase