A monoclonal antibody recognizing apolipoprotein E peptides in systemic amyloid deposits.
A monoclonal antibody was produced by immunization of mice with a crude amyloid A (AA) fibril preparation. This antibody (YK-2), when used in immunohistochemical studies, showed positive staining of amyloid deposits in tissues from patients with systemic amyloidosis (three idiopathic, three myeloma-associated, five reactive, one familial amyloid polyneuropathy, and three dialysis-associated). Immunoblotting revealed that YK-2 reacted with 14,000 and 7,000 dalton components of AA fibrils and a 36,000 dalton component of normal human serum. Absorption with purified human apolipoprotein E abolished staining. This suggests that fragments of apolipoprotein E are a common constituent of amyloid fibril of diverse origins, and it can be a new marker for immunohistochemical studies of systemic amyloidosis.
Yamada
T
T
Department of Laboratory Medicine, Niigata University School of Medicine, Japan.
Kakihara
T
T
Gejyo
F
F
Okada
M
M
D000686:Amyloidosis / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001057:Apolipoproteins E / Q000276:immunology*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D000685:Serum Amyloid A Protein / Q000032:analysis / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D001057:Apolipoproteins E; D000685:Serum Amyloid A Protein; D014357:Trypsin