title: [The isolation of a reassortant strain of the A/Kiev/59/79, R (H1N1) influenza virus and its genomic characteristics].
abstract: As a result of two successive recombinations of influenza A viruses: A/Leningrad/0139/76 (H3N2) with A/PR/8/34 (H1N1) strain and the resulting reassortant A/Leningrad/0139/76, R (H3N2) with a field isolate A/Kiev/59/79 (H1N1), a virus. A/Kiev/59/79, R (H1N1) was obtained. It was established by electrophoresis in polyacrylamide gel and sequencing of kDNA fragments of its genome that the genes PB2, PB1 and NP of this virus belonged to serosubtype H3N2, while genes PA, M, and NS to serosubtype H1N1 (like A/PR/8/34). The surface glycoproteins HA and NA belonged to serosubtype H1N1/like A/USSR/90/77.
authors.forename: T A
authors.initials: TA
authors.lastname: Ermachenko
authors.forename: N E
authors.initials: NE
authors.lastname: Gorev
authors.forename: L G
authors.initials: LG
authors.lastname: Iukhnova
authors.forename: N A
authors.initials: NA
authors.lastname: Petrov
authors.forename: D B
authors.initials: DB
authors.lastname: Golubev
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification*; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral
vernacular_title: Poluchenie reassortantnogo shtamma virusa grippa A/Kiev/59/79, R (H1N1) i kharakteristika ego genoma.