Comparative study of 2',5'-oligoadenylate (2-5A) synthetase in control and Alzheimer's disease (AD) cells.
The properties of 2-5A synthetase, an interferon (IFN)-inducible enzyme that catalyzes the synthesis of a natural nucleic acid with 2',5'-phosphodiester linkage, were investigated using extracts prepared from fibroblasts derived from AD and age/sex-matched controls. Significant differences were found in kinetic parameters as well as activator requirements for the synthetase from the two sources. Fractionation of control and AD total lysates, the postribosomal supernatants, and the ribosomal salt washes, by gel filtration or by glycerol gradient centrifugation, also revealed a substantially different profile of elution of the enzyme. These results further support an altered 2-5A synthetase gene expression in AD cells.
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Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
Wu
J M
JM
D015088:2',5'-Oligoadenylate Synthetase / Q000737:chemistry / Q000378:metabolism*; D000544:Alzheimer Disease / Q000201:enzymology* / Q000235:genetics; D002458:Cell Fractionation; D002478:Cells, Cultured; D004789:Enzyme Activation; D004790:Enzyme Induction; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012330:RNA, Double-Stranded / Q000494:pharmacology
D012330:RNA, Double-Stranded; D015088:2',5'-Oligoadenylate Synthetase