[Characterization of human laryngeal epithelial cells transfected with human papillomavirus type 16 DNA].
Human papillomaviruses (HPVs) are associated with tumor lesions of the larynx. In order to develop a model for investigating the role of HPV in human laryngeal carcinogenesis, normal human laryngeal epithelial cells (HLEC cells) were transfected with a cloned full-length HPV genome. The HPV type 16 (HPV-16) most often associated with cancer of the larynx induced an extended life-span in vitro of HLEC cells by exceeding 100 population doublings, while the HPV-11 that is associated with benign laryngeal papillomas failed to do so. The extended life-span cells (HLEC-16 cells) contained integrated HPV-16 DNA, expressed viral E6 and E7 mRNA, showed anchorage-dependent growth and were non-tumorigenic. Using indirect immunocytochemical staining, nuclear p53 protein was not detectable in any of the HLEC-16 cells, implying that HLEC -16 cells were negative for p53 gene mutations. HLEC-16 cells should be useful for studying mechanisms involved in proliferation, differentiation and neoplastic transformation of HLEC cells.
Iwatake
H
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Department of Otorhinolaryngology, St. Marianna University School of Medicine, Kawasaki.
D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D004847:Epithelial Cells; D006801:Humans; D007830:Larynx / Q000166:cytology*; D027383:Papillomaviridae / Q000235:genetics*; D014162:Transfection
D004279:DNA, Viral