Characterization of phospholipidic components of the natural pulmonary surfactant Curosurf.
The phospholipidic classes of the natural pulmonary surfactant Curosurf were separated by bidimensional TLC and isolated. Quantitative analysis of each class was made by colorimetric determination of phosphorus. FD/MS and NH3-CI/MS were used to identify individual components. GC was used for qualitative/quantitative analysis of fatty acid moieties as methyl esters, using internal standards, by comparison with authentic samples.
Redenti
E
E
Chemical and Biopharmaceutical Department, Chiesi Farmaceutici SpA, Parma, Italy.
Peveri
T
T
Ventura
P
P
Zanol
M
M
Selva
A
A
D000818:Animals; D001688:Biological Products; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D005227:Fatty Acids / Q000032:analysis; D013058:Mass Spectrometry; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000737:chemistry*; D011663:Pulmonary Surfactants / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D001688:Biological Products; D005227:Fatty Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D011663:Pulmonary Surfactants; C068291:poractant alfa