title: Evaluation of the in vitro susceptibility and emergence of mutants resistant to ciprofloxacin among multidrug resistant clinical isolates.
abstract: Two hundred and seventy-seven multidrug resistant clinical isolates [K. pneumoniae, (N = 87); E coli, (N = 30); Salmonella typhimurium (N = 100); P. aeruginosa, (N = 30); S. aureus, (N = 30)] from hospitalized patients specimens, were tested in vitro for sensitivity to Ciprofloxacin. Application of the disk diffusion test and determination of the minimal inhibitory concentration by the microdilution method indicated that, almost all isolates were sensitive to the drug. Overall, S. aureus and P. aeruginosa were the less sensitive organisms. Ciprofloxacin-resistant mutants occurred at frequencies of > or = 10(-5)/CFU.
authors.affiliation: Departamento de Microbiologia e Immunologia, Botucatu, São Paulo (UNESP)-CEP, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Lopes
authors.forename: R P
authors.initials: RP
authors.lastname: Mendes
authors.forename: P C
authors.initials: PC
authors.lastname: Curi
authors.forename: D S
authors.initials: DS
authors.lastname: Watanabe
authors.forename: R M
authors.initials: RM
authors.lastname: Decarlis
authors.forename: A
authors.initials: A
authors.lastname: Horácio
authors.forename: L A
authors.initials: LA
authors.lastname: Michelin
authors.forename: B A
authors.initials: BA
authors.lastname: Placidelli
mesh_terms: D001938:Brazil; D002939:Ciprofloxacin / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012480:Salmonella Infections / Q000382:microbiology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D002939:Ciprofloxacin