Preparation and antigenic properties of digitoxin-bovine serum albumin conjugates linked at the digitoxose C-3' and C-3" positions.
In order to obtain specific antisera to digitoxin, four new types of hapten-bovine serum albumin (BSA) conjugates were synthesized from digitoxin. The haptens were linked to the carrier protein through hemisuccinate and hemisuccinylglycine bridges at the C-3' and C-3" positions in the digitoxose chain. The antisera were prepared by immunizing rabbits with each digitoxin-BSA conjugate and the properties of the antisera were investigated by RIA with 3H-labeled digitoxin. Among these antisera, the antiserum raised against digitoxin 3'-hemisuccinate-BSA conjugate possessed high specificity for digitoxin, exhibiting only minor cross-reactions with digitoxigenin bisdigitoxoside (4.0%), dihydrodigitoxin (2.8%), digoxin (2.4%), digitoxigenin monodigitoxoside (0.23%) and digitoxigenin (< 0.05%).
Fujii
Y
Y
Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Ikeda
Y
Y
Fujii
M
M
Yamazaki
M
M
D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D003432:Cross-Linking Reagents; D004074:Digitoxin / Q000737:chemistry / Q000276:immunology*; D006241:Haptens; D006601:Hexoses / Q000737:chemistry*; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000276:immunology*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D003432:Cross-Linking Reagents; D006241:Haptens; D006601:Hexoses; D007106:Immune Sera; D012710:Serum Albumin, Bovine; C020615:digitoxose; D004074:Digitoxin