title: Rate limitation within a single enzyme is directly related to enzyme intermediate levels.
abstract: The extents to which different rate constants limit the steady-state rate of an isolated enzyme can be quantified as the control coefficients of those constants and elemental steps. We have found that the sum of the control coefficients of rate constants characterising unidirectional rates depleting a particular enzyme intermediate is equal to the concentration of that enzyme intermediate as a fraction of the total enzyme concentration. Together with simple measurements this powerful relation may be used (i) to estimate certain enzyme intermediate levels, in particular the free enzyme concentration, and (ii) to estimate the control coefficients of rate constants and steps.
authors.affiliation: E.C. Slater Institute, University of Amsterdam, The Netherlands.
authors.forename: B N
authors.initials: BN
authors.lastname: Kholodenko
authors.forename: H V
authors.initials: HV
authors.lastname: Westerhoff
authors.forename: G C
authors.initials: GC
authors.lastname: Brown
mesh_terms: D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical
chemical_list: D004798:Enzymes