Identification of a latent MAP kinase kinase kinase in PC12 cells as B-raf.
A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after stimulation of PC12 cells with nerve growth factor or epidermal growth factor, but this is not accompanied by activation of the Mono Q-purified enzyme whether assayed in the absence or presence of GTP-Ras.
Traverse
S
S
Department of Biochemistry, University of Dundee, Scotland, UK.
Cohen
P
P
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange; D004789:Enzyme Activation; D020930:MAP Kinase Kinase Kinases; D008969:Molecular Sequence Data; D016716:PC12 Cells; D010766:Phosphorylation; D011233:Precipitin Tests; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf; D051381:Rats
D011518:Proto-Oncogene Proteins; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf; D020930:MAP Kinase Kinase Kinases