Spontaneous production of interleukin-8 by human lung cancer cells and its augmentation by tumor necrosis factor alpha and interleukin-1 at protein and mRNA levels.
A cell-to-cell interaction between tumors and host inflammatory cells is important for the subsequent cancer progression or regression. We examined the expression of interleukin-8 (IL-8) mRNAs by 9 human lung cancer cell lines and the influences of cytokines on IL-8 production and its gene expression. Substantial expressions of IL-8 gene were detected in 3 lung cancer cell lines (RERF-LC-OK, Lu-134-A-H, YO-88 cells). Moreover, 4 lung cancer cell lines (RERF-LC-MS, RERF-LC-OK, A549 and YO-88) were used to examine the effects of exogenous cytokines--interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha (TNF-alpha) and granulocyte-macrophage colony-stimulating factor--on IL-8 production by the cells at protein and gene levels. TNF-alpha and IL-1 beta significantly augmented the levels of mRNA expression for IL-8 and its production. These observations indicate that tumor-derived IL-8 may be important in recruiting inflammatory neutrophils and promoting interaction between lung cancer and inflammatory cells.
Mizuno
K
K
3rd Department of Internal Medicine, University of Tokushima School of Medicine, Japan.
Sone
S
S
Orino
E
E
Mukaida
N
N
Matsushima
K
K
Ogura
T
T
D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D016209:Interleukin-8 / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D007375:Interleukin-1; D016209:Interleukin-8; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha