title: The dose-dependent effect of Tołpa Peat Preparation on the humoral response of mice immunized with sheep erythrocytes.
abstract: The Balb/c strain mice were immunized by single intraperitoneal administration of 0.2 cm3 of 10% sheep erythrocyte suspension (SRBC). Tołpa Peat Preparation (TPP) used in the study was a peat-derived substance obtained in prof. St. Tołpa's laboratory. It was given to mice intraperitoneally, 4 times at 24-hour intervals. The first dose was administered 2 hours before immunization. Testing was performed with the following doses: 0.5, 2.5, 5, 10, 25, 50, 100 and 250 mg/kg. The determinations comprised the number of spleen cells producing anti-SRBC haemolysins, the level of serum haemagglutinins to SRBC (19S+7S and 7S types) and the percentage of splenocytes forming spontaneous E-rosettes. It was found that the effect of TPP on these immunologic parameters of the mice immunized with SRBC was dose-dependent. The effect potentiating the humoral response occurred for the doses of 0.5-10 mg/kg. The doses of 25 and 50 mg/kg did not affect the immunologic response to SRBC, while the doses of 100 and 250 mg/kg had a suppressive effect. The impact of TPP on the percentage of splenocytes forming E-rosettes was also dose-dependent, but in this case stimulating activity was observed for the doses of 2.5-25 mg/kg.
authors.affiliation: Department of Pharmacology and Toxicology, Agriculture University, Wrocław, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Obmińska-Domoradzka
authors.forename: M
authors.initials: M
authors.lastname: Switała
authors.forename: J
authors.initials: J
authors.lastname: Debowy
authors.forename: T
authors.initials: T
authors.lastname: Garbulński
mesh_terms: D000276:Adjuvants, Immunologic; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002241:Carbohydrates / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D004338:Drug Combinations; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006388:Hemagglutinins / Q000097:blood; D006812:Humic Substances / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012397:Rosette Formation; D012756:Sheep; D012987:Soil; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D014574:Uronic Acids / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D000596:Amino Acids; D002241:Carbohydrates; D004338:Drug Combinations; D006388:Hemagglutinins; D006812:Humic Substances; D012987:Soil; C084029:Tolpa; D014574:Uronic Acids