[Electron-microscopic study of chromatin in nuclei of canine cardiomyocytes as affected by adrenaline].
The study of the chromatin's ultrastructure in the nuclei of cardiomyocytes under the adrenalin influence is very essential (e. g., the square of the perimembrane chromatin-PmCh). The experiments were carried out on 10 male-dogs. Experimental dogs had been receiving adrenalin intravenously--100 mkg per 1 kg of weight during 5 days. The pieces of tissue were fixed by 2.5 percent glutaraldehyde solution ("Serva"-USA) and were postfixed by 2 percent four-oxide osmium, were dehydrated in spirits and acetone, were poured into epoxide mixtures. Microscopic sections were examined with electronic microscope IEM-7 (Japan) increase in 10,000 times. Morphometrical working was carried out by Student method and expressed in conventional units. The PmCh area's relation to the nucleus's area in cardiomyocytes of the controlled animals, equal to 0.10 (10%) and of the experimental ones-0.05 (5%). Thus, the difference in 2 times (P < 0.001) discovered by the electronic microscopy method in dense PmCh areas of cardiomyocytes' nuclei at controlled and experimental animals evidently associated with chromatin's reorganization.
Kliasheva
R I
RI
D000818:Animals; D002843:Chromatin / Q000187:drug effects* / Q000648:ultrastructure; D004285:Dogs; D004837:Epinephrine / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D008297:Male; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure
D002843:Chromatin; D004837:Epinephrine
Elektronno-mikroskopicheskoe issledovanie khromatina v iadrakh kardiomiotsitov sobaki pod vliianiem adrenalina.