Identification of African horse sickness virus in cell culture using a digoxigenin-labeled RNA probe.
A digoxigenin-labeled RNA probe was synthesized from a plasmid containing a portion of the African horse sickness virus (AHSV) serotype 4 genome segment coding for nonstructural protein 1. In an in situ hybridization procedure, this probe hybridized successfully to Vero cells infected with each of the 9 serotypes of AHSV. There was no hybridization with noninfected cell cultures or cell cultures infected with bluetongue virus.
Brown
C C
CC
Foreign Animal Disease Diagnostic Laboratory, USDA, Greenport, NY 11944.
Meyer
R F
RF
Grubman
M J
MJ
D000356:African Horse Sickness Virus / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D002478:Cells, Cultured; D004076:Digoxigenin; D017403:In Situ Hybridization / Q000662:veterinary; D015347:RNA Probes; D012367:RNA, Viral / Q000032:analysis*; D014709:Vero Cells / Q000382:microbiology
D015347:RNA Probes; D012367:RNA, Viral; D004076:Digoxigenin