Sexing bovine embryos using PCR amplification of bovine SRY sequence.
This study analyses the bovine SRY DNA sequence by direct sequencing procedure, followed by the designation of the PCR primers specific for bovine SRY. Using PCR amplification of bovine SRY gene, the embryo sex was determined. The results of the embryo sex identification were confirmed after the embryo transfer and pregnancies.
Zeng
Y T
YT
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, PRC.
Zhang
M L
ML
Chen
M J
MJ
Zhou
X D
XD
Huang
Y
Y
Ren
Z R
ZR
Huang
S Z
SZ
Hu
M X
MX
Wu
X Q
XQ
Gao
J M
JM
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics*; D004624:Embryo Transfer; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis; D014998:Y Chromosome
D004247:DNA