Fibre-optic biosensor for hypoxanthine and xanthine based on a chemiluminescence reaction.
Fibre-optic biosensors were constructed for determination of hypoxanthine and xanthine. Xanthine oxidase and peroxidase were immobilized on different preactivated membranes which were subsequently mounted onto the tip of a fibre-optic bundle. The H2O2 generated by the reaction of hypoxanthine and xanthine oxidase was measured by chemiluminescence (CL) detection using luminol and peroxidase. A linear calibration curve of the sensors in the range of 1-316 microM hypoxanthine and 3.1-316 microM xanthine, respectively, with a detection limit of 0.55 microM hypoxanthine was obtained. Recovery of hypoxanthine ranged between 91 and 102%.
Hlavay
J
J
Department of Analytical Chemistry, University of Veszprém, Hungary.
Haemmerli
S D
SD
Guilbault
G G
GG
D015374:Biosensing Techniques; D002021:Buffers; D006863:Hydrogen-Ion Concentration; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000032:analysis*; D008163:Luminescent Measurements; D019820:Xanthine; D014970:Xanthines / Q000032:analysis*
D002021:Buffers; D007042:Hypoxanthines; D014970:Xanthines; D019820:Xanthine; D019271:Hypoxanthine