Fusion of phospholipid vesicles induced by clathrin: modulation of fused liposome size.
The effects of lipid composition, temperature, and ionic strength on critical events in the membrane fusion reaction induced by clathrin, such as membrane binding and self-association of clathrin, membrane aggregation, and actual fusion, and the size of fused liposomes, were examined using large unilamellar vesicles (LUV) containing acidic phospholipids. When membrane fusion and aggregation of LUV with different lipid compositions were initiated at 25 degrees C in 0.1 M NaCl by lowering the pH of the medium from 7.40 to 4.75 in the presence of clathrin, two types of reaction processes were observed: fusion and aggregation of LUV containing phosphatidylserine and phosphatidic acid occurred slowly with a long fusion lag-phase and reached a high level, whereas the two reactions of LUV containing phosphatidylglycerol and phosphatidylinositol were induced faster with a much shorter fusion lag-time and leveled off in a shorter time. Similar differences in the fusion reactions were observed in media at different temperatures and ionic strengths with any type of LUV: slow and extensive aggregation and fusion occurred at low temperature and/or high ionic strength, but faster, less extensive reactions occurred at high temperature and/or low ionic strength, indicating that the two types of reaction pattern are due to the dependencies of the aggregation and fusion reactions on the temperature and ionic strength, but not on the lipid composition.(ABSTRACT TRUNCATED AT 250 WORDS)
Yoshimura
T
T
Institute for Enzyme Research, University of Tokushima.
Maezawa
S
S
Kameyama
K
K
Takagi
T
T
D002966:Clathrin / Q000737:chemistry*; D008081:Liposomes; D008561:Membrane Fusion; D008563:Membrane Lipids / Q000737:chemistry*; D009994:Osmolar Concentration; D010743:Phospholipids / Q000737:chemistry*; D013696:Temperature
D002966:Clathrin; D008081:Liposomes; D008563:Membrane Lipids; D010743:Phospholipids