Effects of metadoxine on cellular free fatty acid levels in ethanol treated rats.
Free fatty acids (FFA) and fatty-acid ethyl esters (FAEE) were extracted from different organs of rat after ethanol administration. The results showed that ethanol treatment increased the concentration of saturated and mono-unsaturated FFA and FAEE while decreasing the poly-unsaturated fatty-acid content. Pretreatment of animals with Metadoxine one hour before ethanol administration inhibited the increased formation of saturated and mono-unsaturated FFA. While ethanol administration induced a marked increase in glutathione (GSH) S-transferase activity correlated with increased formation of FAEE in different rat tissues examined, pretreatment of such rats with Metadoxine reduced considerably the increased enzyme activity of their GSH S-transferase. Elevated concentrations of saturated and mono-unsaturated FFA conceivably reflect changes in the composition of lipid membranes. Hence, the cellular response to ethanol would be the formation of highly-packed rigid membrane structures. Pretreatment with Metadoxine causes an inhibition in the formation of elevated quantities of saturated and mono-unsaturated fatty acids. This condition could result in a lower degree of adaptability to ethanol intake and perhaps a decreased level of tolerance to ethanol.
Calabrese
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V
Institute of Biochemistry, University of Catania School of Medicine, Italy.
Bombaci
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G
Calderone
A
A
Rizza
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V
D000818:Animals; D002477:Cells / Q000187:drug effects / Q000378:metabolism; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical; D000431:Ethanol / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D008297:Male; D011736:Pyridoxine / Q000494:pharmacology*; D011761:Pyrrolidonecarboxylic Acid / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D004338:Drug Combinations; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D000431:Ethanol; C037845:metadoxine; D011736:Pyridoxine; D011761:Pyrrolidonecarboxylic Acid