title: A cytochrome ba3 functions as a quinol oxidase in Paracoccus denitrificans. Purification, cloning, and sequence comparison.
abstract: A quinol oxidase has been purified from the cytoplasmic membrane of Paracoccus denitrificans; its heme composition and CO binding properties identify it as a cytochrome ba3. On SDS gels, the purified enzyme complex is separated into five polypeptides. Using partial peptide sequence information for subunit II, the gene locus has been cloned and sequenced. In a typical operon pattern, four genes were identified: qoxA, -B, -C, and -D, coding for subunits II, I, III, and IV. DNA-derived amino acid sequence comparisons reveal extensive similarities to other members of the terminal oxidase superfamily.
authors.affiliation: Department of Molecular Genetics, University of Frankfurt, Federal Republic of Germany.
authors.forename: O M
authors.initials: OM
authors.lastname: Richter
authors.forename: J S
authors.initials: JS
authors.lastname: Tao
authors.forename: A
authors.initials: A
authors.lastname: Turba
authors.forename: B
authors.initials: B
authors.lastname: Ludwig
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D003300:Copper / Q000378:metabolism; D003429:Cross Reactions; D003573:Cytochrome b Group / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial; D003576:Electron Transport Complex IV / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006418:Heme / Q000378:metabolism; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism*; D010231:Paracoccus denitrificans / Q000201:enzymology*; D010449:Peptide Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D003573:Cytochrome b Group; D004269:DNA, Bacterial; D006418:Heme; D003300:Copper; D010088:Oxidoreductases; C058557:cytochrome ba3; C020094:duroquinol oxidase; D003576:Electron Transport Complex IV