[Quantification of the enantiomers of ofloxacin in human urine by RP-HPLC with chiral mobile phase additive].
The method for determination of S-(-) and R-(+)-ofloxacin (OFLX) in human urine by RP-HPLC with a chiral mobile phase additive is described. L-phenylalanine was used as the chiral additive, Cu2+ as the ligand ion and fluorescence for detection. This chiral ligand exchange chromatography has been applied to reveal the enantioselective disposition of ofloxacin in human volunteers.
Zeng
S
S
School of Pharmacy, Zhejiang Medical University, Hangzhou.
Zhang
L
L
Liu
Z Q
ZQ
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D015242:Ofloxacin / Q000652:urine*; D013237:Stereoisomerism
D015242:Ofloxacin