title: Interleukin 1 is released by murine macrophages during apoptosis induced by Shigella flexneri.
abstract: Peritoneal macrophages undergoing apoptosis induced by Shigella flexneri infection release the inflammatory cytokine interleukin 1 (IL-1), but not IL-6 or tumor necrosis factor alpha (TNF alpha). Wild type shigella causes a very fast and significant release of IL-1 from prestimulated peritoneal macrophages, before the cell's integrity is compromised. Both IL-1 alpha and IL-1 beta are released, IL-1 beta in its mature processed form. IL-1 is released from presynthesized cytoplasmic pools. These results demonstrate that bacteria-induced apoptosis of macrophages may play an active role in vivo by releasing IL-1, which in turn mediates an early inflammatory response in epithelial tissues.
authors.affiliation: Unité de Pathogénie Microbienne Moléculaire, Institut National de la Santé et de la Recherche Médicale U389, Institut Pasteur, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Zychlinsky
authors.forename: C
authors.initials: C
authors.lastname: Fitting
authors.forename: J M
authors.initials: JM
authors.lastname: Cavaillon
authors.forename: P J
authors.initials: PJ
authors.lastname: Sansonetti
mesh_terms: D000818:Animals; D017209:Apoptosis; D001681:Biological Assay; D002478:Cells, Cultured; D004926:Escherichia coli; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000382:microbiology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012763:Shigella flexneri / Q000472:pathogenicity*
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides