title: Amisulpride evaluation by radioreceptor assay comparison with HPLC procedure after single administration in the rabbit.
abstract: A radioreceptor assay (RRA) was developed to determine plasma levels of amisulpride. In in vitro binding conditions, the RRA technique was sensitive (11.6 nmol/l) and reliable, with a high degree of precision. The reproducibility of the method through statistical coefficients was < 7%. This method was applied to a pilot pharmacokinetic study in rabbits following single i.v. administration of amisulpride at 10 mg/kg. The results obtained were compared with those obtained by high performance liquid chromatography method (HPLC) in order to assess the validity of this new RRA technique.
authors.affiliation: Laboratoire de Pharmacologie et Pharmacocinétique, Faculté de Pharmacie, Lille, France.
authors.forename: R
authors.initials: R
authors.lastname: Mokrim
authors.forename: C
authors.initials: C
authors.lastname: Brunet
authors.forename: M
authors.initials: M
authors.lastname: Cazin
authors.forename: B
authors.initials: B
authors.lastname: Gressier
authors.forename: M
authors.initials: M
authors.lastname: Luyckx
authors.forename: T
authors.initials: T
authors.lastname: Dine
authors.forename: H
authors.initials: H
authors.lastname: Robert
authors.forename: J C
authors.initials: JC
authors.lastname: Cazin
mesh_terms: D000077582:Amisulpride; D000818:Animals; D014150:Antipsychotic Agents / Q000493:pharmacokinetics* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D065127:Dopamine D2 Receptor Antagonists; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D013134:Spiperone / Q000493:pharmacokinetics; D013469:Sulpiride / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D014150:Antipsychotic Agents; D065127:Dopamine D2 Receptor Antagonists; D017448:Receptors, Dopamine D2; D013134:Spiperone; D013469:Sulpiride; D000077582:Amisulpride