Comparative analysis of the region of the mitochondrial genome containing the ATPase subunit 9 gene in the two related yeast species Saccharomyces douglasii and Saccharomyces cerevisiae.
We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequence with that of S. cerevisiae reveals the presence of three silent transitions. A high level of similarity is also found between regions involved in the initiation of transcription and mRNA processing. More interestingly, a region of similarity situated outside the known regulatory regions has been identified. As the intergenic regions are generally highly divergent, the remarkable conservation of these non-coding sequences suggests that their structure may be relevant to the expression of this region of the mitochondrial DNA.
Nicoletti
L
L
Dipartimento di Chimica Biologica, Università di Padova, Italy.
Laveder
P
P
Pellizzari
R
R
Cardazzo
B
B
Carignani
G
G
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012323:RNA Processing, Post-Transcriptional; D012440:Saccharomyces / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity; D014158:Transcription, Genetic
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D000251:Adenosine Triphosphatases