Structural study on an exocellular polysaccharide produced by Lactobacillus helveticus TY1-2.
Lactobacillus helveticus TY1-2 produced an exocellular polysaccharide when it was cultured in reconstituted skim milk. This polysaccharide is a high molecular weight heteropolymer of D-glucopyranosyl, D-galactopyranosyl, and 2-acetamido-2-deoxy-D-glucopyranosyl residues in the molar ratio 3.0:2.8:0.9. The primary structure of the polysaccharide was shown by glycose analysis, methylation analysis, Smith degradation, and NMR spectroscopy to be composed of branched heptasaccharide repeating units having the following structure: [formula: see text]
Yamamoto
Y
Y
United Graduate School of Agricultural Science, Gifu University, Japan.
Murosaki
S
S
Yamauchi
R
R
Kato
K
K
Sone
Y
Y
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D007778:Lactobacillus / Q000276:immunology / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification
D002241:Carbohydrates; D007202:Indicators and Reagents; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial