Inhibition of cell adhesion by microspheres coated with recombinant soluble intercellular adhesion molecule-1.
Murine recombinant soluble intercellular adhesion molecule-1 (sICAM-1) was produced and characterized. When immobilized on plastic microtiter wells, sICAM-1 efficiently mediated LFA-1-dependent cell adhesion, indicating that the purified protein retained the ability to bind to LFA-1. However, sICAM-1 in solution, at concentrations up to 100 micrograms/ml, was incapable of inhibiting the phorbol ester-induced homotypic aggregation of lymphocytes, the adhesion of T cells to plastic immobilized sICAM-1, and CTL effector function, all of which are mediated by intercellular adhesion molecule-1:LFA-1 interaction. In contrast, uniform polystyrene microspheres coated with sICAM-1 bound specifically to LFA-1+ cells and efficiently inhibited the adhesion of T cells to immobilized sICAM-1. The sICAM-1-coated microspheres also inhibited CTL function, but the inhibition was only partial. These results suggest that although monomeric sICAM-1 cannot competitively inhibit cell adhesion mediated by intercellular adhesion molecule-1 and LFA-1, microspheres coated with sICAM-1 can inhibit such cell adhesion.
Welder
C A
CA
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada.
Lee
D H
DH
Takei
F
F
D000818:Animals; D001667:Binding, Competitive; D002448:Cell Adhesion / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000494:pharmacology*; D002449:Cell Aggregation / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D018799:Intercellular Adhesion Molecule-1; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D051379:Mice; D008863:Microspheres; D011137:Polystyrenes; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D012995:Solubility; D012996:Solutions; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology
D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D011137:Polystyrenes; D011994:Recombinant Proteins; D012996:Solutions; D018799:Intercellular Adhesion Molecule-1