New developments in stem cell detection and stem cell selection in the context of autologous transplantations.
The concept of bone marrow transplantation is now enlarged to hemopoietic stem cell transplantation. Basic research on hemopoiesis, its regulation and markers, allowed concrete progress in the field of autologous transplantation. We report two applications: 1) the improvement of methods for detection of stem cells, which ensure engraftment, using recombinant growth factors, allowed a more sensitive determination of chemotherapeutic in vitro treatments toxicity upon normal progenitor cells; 2) large scale selection of hemopoietic progenitor/stem cell using the CD 34 immunological marker: its application to autologous transplantation.
Douay
L
L
Unité de Recherche sur les greffes de cellules souches hématopoiétiques, CHU Saint Antoine, Paris, France.
Lopez
M
M
Giarratana
M C
MC
Bony
V
V
Bouchet
S
S
Gorin
N C
NC
D016465:Bone Marrow Purging / Q000379:methods; D016026:Bone Marrow Transplantation / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D014182:Transplantation, Autologous