Evidence that two non-overlapping high-affinity calmodulin-binding sites are present in the head region of synapsin I.
Calmodulin is an important element in the regulation of nerve terminal exocytosis by Ca2+. Calmodulin has been shown to interact with the synaptic vesicle phosphoproteins synapsins Ia and Ib [Okabe, T. & Sobue, K. (1987) FEBS Lett. 213, 184-188; Hayes, N. V. L., Bennett, A. F. & Baines, A. J. (1991) Biochem. J. 275, 93-97]. These proteins are thought to provide regulated linkages between synaptic vesicles and cytoskeletal elements. It is well established that calmodulin modulates synapsin I activities via calmodulin-dependent protein-kinase-II-catalysed phosphorylation. The direct binding of calmodulin to synapsin I suggests a second mode of regulation in addition to phosphorylation. In this study, we present evidence indicating that two sites for calmodulin binding exist in the N-terminal head region of synapsins Ia and Ib. In unphosphorylated synapsin I, these sites had a Kd value of = 36 +/- 14 nM for binding to calmodulin labelled with acetyl-N'-(5-sulpho-1-naphthyl)ethylene diamine. The Kd values for synapsin I phosphorylated at various sites were as follows: site I 18 +/- 11 nM; sites II and III 35 +/- 14 nM; sites I-III 16 +/- 9 nM. The fluorescence data indicated a stoichiometry of not less than 2 mol calmodulin bound to 1 mol synapsin I at saturation in each case. Consistent with this stoichiometry, two chemically cross-linked species (96 kDa and 116 kDa) containing calmodulin and synapsin I were generated in vitro, corresponding to one and two calmodulin molecules bound/synapsin I. Defined fragments of synapsin I were generated with the reagent 2-nitro-5-thiocyanobenzoic acid, which cleaves at cysteine residues. Cysteine-specific cleavage of whole synapsin I after cross-linking to biotinylated calmodulin generated a pair of polypeptide complexes (approximately 46 kDa and 38 kDa), the masses of which indicated cross-linking of calmodulin to the N-terminal and middle regions of synapsin I. Purified N-terminal and middle fragments each showed a Ca(2+)-dependent interaction with calmodulin affinity columns. Two calmodulin-binding fragments (7.4 kDa and 6.5 kDa) were generated using Staphylococcus aureus V8 protease digestion of synapsin I. These fragments were isolated by calmodulin affinity chromatography and reverse-phase HPLC. N-terminal sequence analysis indicated that each was contained within one of the 2-nitro-5-thiocyanobenzoic-acid-derived calmodulin-binding fragments.(ABSTRACT TRUNCATED AT 400 WORDS)
Goold
R
R
Biological Laboratory, University of Kent, England.
Baines
A J
AJ
D000199:Actins / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002147:Calmodulin / Q000378:metabolism*; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism; D009282:Naphthalenesulfonates / Q000378:metabolism; D010766:Phosphorylation; D011817:Rabbits; D012697:Serine Endopeptidases / Q000378:metabolism; D012756:Sheep; D016704:Synapsins / Q000737:chemistry / Q000378:metabolism*; D013861:Thiocyanates / Q000737:chemistry
C032162:AEDANS-calmodulin; D000199:Actins; D002147:Calmodulin; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D009282:Naphthalenesulfonates; D016704:Synapsins; D013861:Thiocyanates; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; C008658:2-nitro-5-thiocyanobenzoic acid