Characterization of phosphoinositol oligosaccharides from parasitic protozoa by fast atom bombardment and collisional activation mass spectrometry.
Fast atom bombardment mass spectrometry (FAB MS) enables the rapid, accurate and sensitive determination of the molecular masses of glycosylphosphatidylinositol (GPI)-derived oligosaccharides, from which the composition in terms of monosaccharide residues and non-carbohydrate substituents can be determined. Interpretation of fragment ions in collisional activation mass spectra further enables the determination of residue sequence, the positions of branch points, and the location of non-carbohydrate substituents. We have applied these techniques to the characterization of phosphoinositol oligosaccharides from Leptomonas samueli, Endotrypanum schaudinni and Leishmania adleri. The mass spectral data permit the postulation of candidate structures for the oligosaccharides, which provide a set of constraints that can assist the interpretation of results from other techniques such as NMR.
Wait
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Division of Pathology, PHLS Centre for Applied Microbiology and Research, Salisbury, UK.
Jones
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Previato
J O
JO
Mendonça-Previato
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L
D000818:Animals; D002240:Carbohydrate Sequence; D017261:Glycosylphosphatidylinositols / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D015800:Protozoan Proteins / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014351:Trypanosomatina / Q000737:chemistry*
D017261:Glycosylphosphatidylinositols; D009844:Oligosaccharides; D015800:Protozoan Proteins