title: Evaluation of the new Cobas Core IgE Total EIA in comparison to three commercial IgE immunoassays.
abstract: Determination of total IgE by the new Cobas Core IgE Total EIA was compared with three other routine methods (Pasteur IgE ELISA, Pasteur IgE RIA and Pharmacia IgE Ultra-PRIST). Mean IgE concentrations in adult sera correlated well among the three methods (Cobas Core IgE Total EIA, Pasteur IgE ELISA and Pasteur IgE RIA); however, the values for the Pasteur IgE RIA were on average 20-50 IU/ml higher than those measured by the enzyme immunoassays. The correlation between the Cobas Core IgE Total EIA and the Pharmacia IgE Ultra-PRIST in sera from newborns was also excellent. The analytical performance of the Cobas Core IgE Total EIA in terms of dilution linearity, recovery and intra-assay reproducibility (coefficient of variation = 1.8-3.8%) was excellent.
authors.affiliation: Institute of Immunology, Inselspital, Bern, Switzerland.
authors.forename: A L
authors.initials: AL
authors.lastname: de Weck
authors.forename: M
authors.initials: M
authors.lastname: Schluchter
authors.forename: P
authors.initials: P
authors.lastname: Girardin
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood*; D007231:Infant, Newborn; D011863:Radioimmunoassay
chemical_list: D007073:Immunoglobulin E