Reversible rearrangement of vimentin-type intermediate filaments in cultured human skin fibroblasts from patients with lysosomal storage diseases.
Immunofluorescence microscopy shows that unlike cytoplasmic microtubules (MT), vimentin-type intermediate filaments (IF) are collected into ring-shaped structures in affected fibroblasts. The altered IF organization could be observed in monolayers of polarized fibroblasts in the prolonged stationary growth phase and-after replating-upon their initial spreading. Transition from a discoid to an extended cellular form is accompanied by centrifugal dislocation of ring-shaped IF structures towards the cell's active edge with gradual restoration of the radial fibrillar vimentin network. Spreading of affected cells occurred more slowly than that of control fibroblasts.
Tatiana
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I
Institute of Biomedical Chemistry, Moscow, Russia.
German
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W
D002460:Cell Line; D017358:Cell Wall Skeleton / Q000648:ultrastructure; D005347:Fibroblasts / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D007382:Intermediate Filaments / Q000737:chemistry / Q000648:ultrastructure*; D016464:Lysosomal Storage Diseases / Q000473:pathology*; D008856:Microscopy, Fluorescence; D012867:Skin / Q000737:chemistry / Q000648:ultrastructure*; D014746:Vimentin / Q000032:analysis*
D017358:Cell Wall Skeleton; D014746:Vimentin