title: cDNA cloning of a germ cell specific lamin B3 from mouse spermatocytes and analysis of its function by ectopic expression in somatic cells.
abstract: The nuclear lamina is a fundamental component involved in the assembly of the nuclear envelope and higher order chromosomal structures in eukaryotes. In mammals, it is composed of four major lamin proteins, termed lamins A, B1, B2 and C. Here we first report cDNA cloning of a new 53 kDa lamin protein from mouse spermatocytes, termed lamin B3, the expression of which appears restricted to spermatogenic cells. Its gene structure indicates that lamin B3 is generated by differential splicing and alternative polyadenylation from lamin B2. When lamin B3 is introduced into somatic cells in culture, their nuclear morphology is transformed from spherical to hook-shaped. On the basis of the results obtained, we suggest that the germ cell specific lamin B3 is involved in the reorganization of nuclear and chromosomal structures during meiotic division.
authors.affiliation: Department of Biology, School of Science, Nagoya University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
authors.forename: Y
authors.initials: Y
authors.lastname: Hotta
mesh_terms: D000375:Aging / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002467:Cell Nucleus / Q000502:physiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005091:Exons; D005455:Fluorescent Antibody Technique; D007381:Intermediate Filament Proteins; D034921:Lamin Type B; D034882:Lamins; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D013090:Spermatocytes / Q000502:physiology*; D013737:Testis / Q000254:growth & development / Q000502:physiology; D014162:Transfection
chemical_list: D007381:Intermediate Filament Proteins; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D011061:Poly A; D004247:DNA