title: Human cardiac troponin T: identification of fetal isoforms and assignment of the TNNT2 locus to chromosome 1q.
abstract: The troponin complex is located on the thin filament of striated muscle and is composed of three component polypeptides: troponin T, troponin I, and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in cardiac muscle, slow skeletal muscle, and fast skeletal muscle, respectively. Each of these genes is subject to alternative splicing, resulting in the production of multiple tissue-specific isoforms. We have cloned cDNAs encoding human cardiac troponin T from adult heart and have used these to demonstrate that multiple cardiac troponin T mRNAs are present in the human fetal heart, resulting from alternative splicing in the 5' coding region of the gene. Hybridization of the cloned cDNAs to genomic DNA identifies a single-copy gene, and using somatic cell hybrid analysis, we have mapped the corresponding gene locus (designated TNNT2) to the long arm of chromosome 1 (1cen-qter).
authors.affiliation: Department of Cardiothoracic Surgery, National Heart and Lung Institute, London, United Kingdom.
authors.forename: P J
authors.initials: PJ
authors.lastname: Townsend
authors.forename: H
authors.initials: H
authors.lastname: Farza
authors.forename: C
authors.initials: C
authors.lastname: MacGeoch
authors.forename: N K
authors.initials: NK
authors.lastname: Spurr
authors.forename: R
authors.initials: R
authors.lastname: Wade
authors.forename: R
authors.initials: R
authors.lastname: Gahlmann
authors.forename: M H
authors.initials: MH
authors.lastname: Yacoub
authors.forename: P J
authors.initials: PJ
authors.lastname: Barton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D005333:Fetus; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D014336:Troponin / Q000235:genetics*; D019209:Troponin C; D019210:Troponin I; D020107:Troponin T; D014714:Vertebrates / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D014336:Troponin; D019209:Troponin C; D019210:Troponin I; D020107:Troponin T