Amisulpride evaluation by radioreceptor assay comparison with HPLC procedure after single administration in the rabbit.
A radioreceptor assay (RRA) was developed to determine plasma levels of amisulpride. In in vitro binding conditions, the RRA technique was sensitive (11.6 nmol/l) and reliable, with a high degree of precision. The reproducibility of the method through statistical coefficients was < 7%. This method was applied to a pilot pharmacokinetic study in rabbits following single i.v. administration of amisulpride at 10 mg/kg. The results obtained were compared with those obtained by high performance liquid chromatography method (HPLC) in order to assess the validity of this new RRA technique.
Mokrim
R
R
Laboratoire de Pharmacologie et Pharmacocinétique, Faculté de Pharmacie, Lille, France.
Brunet
C
C
Cazin
M
M
Gressier
B
B
Luyckx
M
M
Dine
T
T
Robert
H
H
Cazin
J C
JC
D000077582:Amisulpride; D000818:Animals; D014150:Antipsychotic Agents / Q000493:pharmacokinetics* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D065127:Dopamine D2 Receptor Antagonists; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D013134:Spiperone / Q000493:pharmacokinetics; D013469:Sulpiride / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology
D014150:Antipsychotic Agents; D065127:Dopamine D2 Receptor Antagonists; D017448:Receptors, Dopamine D2; D013134:Spiperone; D013469:Sulpiride; D000077582:Amisulpride