title: Enhancing the efficiency of transgene expression.
abstract: Two strategies for enhancing gene expression in transgenic animals are described with particular reference to targeting expression to the mammary gland. Gene constructs in which the protein-encoding DNA sequences are contained within a genomic segment (comprising most or all of the natural introns of the corresponding gene) are shown to be expressed more efficiently than their intronless counterparts. Secondly, co-integrating an otherwise poorly expressed transgene in the vicinity of an actively expressed transgene can dramatically improve its efficiency of expression.
authors.affiliation: AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian, U.K.
authors.forename: A J
authors.initials: AJ
authors.lastname: Clark
authors.forename: A L
authors.initials: AL
authors.lastname: Archibald
authors.forename: M
authors.initials: M
authors.lastname: McClenaghan
authors.forename: J P
authors.initials: JP
authors.lastname: Simons
authors.forename: R
authors.initials: R
authors.lastname: Wallace
authors.forename: C B
authors.initials: CB
authors.lastname: Whitelaw
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D005821:Genetic Techniques; D007438:Introns; D007782:Lactoglobulins / Q000235:genetics; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D014162:Transfection / Q000379:methods*
chemical_list: D007782:Lactoglobulins