title: Effect of serotonin on protein phosphorylation in the central nervous system of the leech Hirudo medicinalis.
abstract: 1. Phosphoproteins of different regions of the Hirudo medicinalis central nervous system have been analysed by means of two-dimensional electrophoresis. 2. Serotonin, 8-Br-cAMP and phorbol 12,13-dibutyrate stimulate phosphorylation of a number of proteins whose isoelectric points and molecular weights are presented. 3. A group of proteins of 78 kDa and pI = 6-6.5, whose level of phosphorylation increases in the presence of serotonin, 8-Br-cAMP and phorbol ester, is observed only in segmental but not in cephalic or caudal ganglia. 4. The putative roles of these phosphoproteins are discussed.
authors.affiliation: Department of Physiology and Biochemistry, University of Pisa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Garcia-Gil
authors.forename: D
authors.initials: D
authors.lastname: Bottai
authors.forename: M L
authors.initials: ML
authors.lastname: Zaccardi
authors.forename: M
authors.initials: M
authors.lastname: Cannizzaro
authors.forename: M
authors.initials: M
authors.lastname: Brunelli
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002490:Central Nervous System / Q000187:drug effects* / Q000378:metabolism; D005724:Ganglia / Q000187:drug effects / Q000378:metabolism; D007865:Leeches / Q000187:drug effects* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010750:Phosphoproteins / Q000032:analysis; D010766:Phosphorylation; D012701:Serotonin / Q000494:pharmacology*
chemical_list: D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D012701:Serotonin; D015240:Phorbol 12,13-Dibutyrate