The alpha and beta thyroid receptors are expressed by cultured ependymal cells. Correlation with the effect of L-3,5,3'-triiodothyronine on glutamine synthetase mRNAs.
It is generally accepted that L-3,5,3'-triiodothyronine (L-T3) acts at the genomic level through an interaction with specific nuclear L-T3 receptors (NT3R). Using antibodies raised against different peptides of NT3R, we report here the immunocytochemical localization of the alpha, alpha 2, beta 1 NT3R subtypes in ependymal cell primary cultures. The alpha and beta thyroid hormone receptors are both expressed. While the alpha and alpha 2 subtypes are found in almost all cells, the beta 1 receptors are present in few cells only. The possibility that alpha and beta receptors are colocalized is discussed. We also demonstrate that ependymal cells respond to L-T3 with a marked increase of the expression of the glutamine synthetase messenger RNAs.
Graff
M N
MN
UPR 416 du CNRS, Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, Strasbourg, France.
Baas
D
D
Puymirat
J
J
Sarlieve
L L
LL
Delaunoy
J P
JP
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001345:Autoradiography; D002478:Cells, Cultured; D004805:Ependyma / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis* / Q000276:immunology; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D011988:Receptors, Thyroid Hormone / Q000187:drug effects* / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology*; D015854:Up-Regulation / Q000502:physiology
D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D005974:Glutamate-Ammonia Ligase