Constitutive expression of the putative transcription factor SCL associated with proviral insertion in the myeloid leukemic cell line WEHI-3BD-.
The murine myeloid leukemia WEHI-3B contains an intracisternal A-particle insertion in a growth factor gene (IL-3) and a homeobox gene (Hox 2.4). Southern blot analysis of the differentiation unresponsive subline WEHI-3BD- demonstrated a rearrangement of the SCL gene, a gene that encodes a protein of the helix-loop-helix class of transcription factors. Northern blot analysis demonstrated an abnormal SCL transcript in the WEHI-3BD- cells, although SCL expression was not detected in the parental cells. The rearrangement was cloned and confirmed as an insertion of an intracisternal A-particle in the 3' untranslated region of the SCL gene. Although SCL is implicated in up to 25% of human T-cell acute lymphoblastic leukemia, this is the first example of SCL involvement in a myeloid leukemia.
Tanigawa
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T
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Robb
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L
Green
A R
AR
Begley
C G
CG
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015342:DNA Probes; D004251:DNA Transposable Elements / Q000235:genetics; D005786:Gene Expression Regulation; D018257:Helix-Loop-Helix Motifs / Q000235:genetics*; D007942:Leukemia, Experimental / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D004251:DNA Transposable Elements; D014157:Transcription Factors