Complementary DNA sequence encoding the major neural cell adhesion molecule isoform in a human small cell lung cancer cell line.
The neural cell adhesion molecule (N-CAM), a member of the immunoglobulin gene super-family mediating homophilic cell-cell adhesion in a neuroendocrine system, is preferentially expressed in human small cell lung cancer (SCLC). Immunoprecipitation of a panel of SCLC cell lines by monoclonal antibodies (mAbs) specific for N-CAM detects mainly the 145-kDa isoform. This result was correlated with Northern blotting where a single 6.2-kb mRNA was detected in nine SCLC cell lines. To determine cDNA sequence encoding the N-CAM isoform, we selected several cDNA clones encoding N-CAM isolated from OS2-R, a SCLC cell line established in our laboratory. Based on the analysis of the full-length cDNA obtained from two clones, the sequence of this 145-kDa isoform was shown to be essentially identical to that of the 140-kDa N-CAM isoform of neuroblastoma except for a single base pair changed at position 1620 without changing amino acid encoded.
Saito
S
S
Department of Internal Medicine III, Osaka University Medical School, Japan.
Tanio
Y
Y
Tachibana
I
I
Hayashi
S
S
Kishimoto
T
T
Kawase
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018288:Carcinoma, Small Cell / Q000737:chemistry*; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry*; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured
D015816:Cell Adhesion Molecules, Neuronal; D018076:DNA, Complementary