Murine cytomegalovirus-associated pneumonitis in the lungs free of the virus.
At 4 wk after intraperitoneal inoculation of murine cytomegalovirus (MCMV) in adult BALB/c mice, MCMV remained detectable only in the salivary glands. When T cells of these mice were activated by a single injection of anti-CD3 epsilon monoclonal antibody, mice died of interstitial pneumonitis at 24-48 h after injection, accompanied by elevation of serum levels of TNF-alpha and IFN-gamma. However, MCMV remained undetectable in the lungs during the period. Simultaneous injection of cyclosporin A reduced such effects of anti-CD3. In conclusion, although the presence of MCMV in the host may be required, MCMV-associated pneumonitis is not mediated by virus in the lung but probably by the cytokines released from T cells, of which responsiveness to stimulation via CD3 molecule has been presumably modified by MCMV infection.
Tanaka
K
K
Department of Virology, Kyushu University, Fukuoka, Japan.
Koga
Y
Y
Lu
Y Y
YY
Zhang
X Y
XY
Wang
Y
Y
Kimura
G
G
Nomoto
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001483:Base Sequence; D017252:CD3 Complex / Q000276:immunology; D016572:Cyclosporine / Q000494:pharmacology; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D003586:Cytomegalovirus Infections / Q000097:blood / Q000382:microbiology / Q000473:pathology*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D007371:Interferon-gamma / Q000097:blood; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011024:Pneumonia, Viral / Q000097:blood / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012469:Salivary Glands / Q000382:microbiology / Q000473:pathology; D013154:Spleen / Q000473:pathology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D017931:DNA Primers; D004279:DNA, Viral; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016572:Cyclosporine