Automated image analysis DNA cytometry in testicular cancer.
The value of automated DNA cytometry for differentiation of testis cancer was evaluated in 54 seminomas, 13 HCG-positive seminomas, and 48 embryonal carcinomas. Slices of paraffin embedded tissue were enzymatically digested and stained with Feulgen SITS after fixation on glass slides. Automated DNA cytometry was performed with a Modular Image Analysis Computer (MIAC). DNA histogram phenotpye and computed DNA indices were correlated with the different tumor types. The ratio of hypertriploid to hypotriploid increased from HCG-positive seminoma over embryonal carcinoma to seminoma. The following mathematical DNA indices were found to correlate with tumor type: mean ploidy, 2c deviation index, 5c exceeding rate, variation coefficient of the GO/1 fraction and DNA nucleus diameter correlation.
Bürger
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RA
Urologische Klinik, Johannes-Gutenberg-Universität, Mainz, Germany.
Witzsch
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U
Engel
H
H
Potratz
D
D
Stöckle
M
M
D018236:Carcinoma, Embryonal / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D010641:Phenotype; D011003:Ploidies; D018239:Seminoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013736:Testicular Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D004273:DNA, Neoplasm