Characterization of genetic markers in the 5'flanking region of the apo A1 gene.
Genetic variation of apo A1/C3/A4 is associated with hyperlipidaemia and coronary heart disease. We report the polymerase chain reaction (PCR) conditions for determining three polymorphic sites in the 5'flanking region of apoA1 using DNA prepared from small aliquots of whole blood. These polymorphisms identify six haplotypes that will be of value in genetic studies.
Shoulders
C C
CC
Division of Molecular Medicine, Royal Postgraduate Medical School, London, UK.
Narcisi
T M
TM
Jarmuz
A
A
Brett
D J
DJ
Bayliss
J D
JD
Scott
J
J
D016632:Apolipoprotein A-I / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D005819:Genetic Markers; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017384:Sequence Deletion
D016632:Apolipoprotein A-I; D005819:Genetic Markers; D004247:DNA