Clinical utility of an enhanced human immunodeficiency virus type 1 p24 antigen capture assay.
The presence of p24 core antigen in the serum of individuals with human acquired immunodeficiency syndrome has been used as one of the important prognostic markers of HIV-1 infection and also as an end point in evaluating antiviral drugs and vaccines. Unfortunately the majority of p24 antigen present in serum exists as an antigen-antibody complex and is not detected with the commercial kits currently available to measure p24 antigen. In this study, we report a simple procedure utilizing treatment of serum samples with glycine buffer (pH 1.85) to dissociate antigen-antibody complexes prior to assaying for p24 antigen. A 300% increase in the number of p24-reactive samples and a 3- to 12-fold increase in the quantity of antigen detected were observed when samples were pretreated with 1.5 M glycine buffer (pH 1.85) for 1 hr. Glycine treatment of samples did not result in non-specific positive tests and samples previously shown to be reactive remained positive. In reconstruction experiments the release of antigen was found to be inversely proportional to the amount of p24 antibody present in the serum. The percentage of HIV-1-infected patients positive for p24 antigen was clearly a function of CD4 count. Forty-nine percent of patients with more than 500 CD4 cells and 100% of patients with less than 200 CD4 were p24 positive.(ABSTRACT TRUNCATED AT 250 WORDS)
Vasudevachari
M B
MB
Department of Microbiology, Georgetown University Medical School, Washington, D.C. 20007.
Salzman
N P
NP
Woll
D R
DR
Mast
C
C
Uffelman
K W
KW
Toedter
G
G
Hoefheinz
D
D
Metcalf
J A
JA
Lane
H C
HC
D000936:Antigen-Antibody Complex; D015496:CD4-Positive T-Lymphocytes; D016655:HIV Core Protein p24 / Q000032:analysis*; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D007958:Leukocyte Count; D012680:Sensitivity and Specificity
D000936:Antigen-Antibody Complex; D016655:HIV Core Protein p24