title: Comparison of E test with agar dilution for antimicrobial susceptibility testing of Neisseria gonorrhoeae.
abstract: A collection of 150 Neisseria gonorrhoeae isolates from Africa, where various resistance mechanisms among N. gonorrhoeae isolates are common, was used to the compare E test (AB Biodisk, Solna, Sweden) with agar dilution susceptibility testing. MICs obtained by the E test agreed within 1 log2 concentration by the agar dilution method for 97.5, 97.3, 96.6, 94, and 84.7% of the tested isolates for penicillin, ciprofloxacin, chloramphenicol, tetracycline, and trimethoprim-sulfamethoxazole, respectively. No significant difference in susceptibility categorization was observed between either method. The E test is an attractive alternative to the agar dilution technique and is a more appropriate method for N. gonorrhoeae susceptibility testing in developing countries.
authors.affiliation: Institute of Tropical Medicine, Division of Microbiology, Antwerp, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Van Dyck
authors.forename: H
authors.initials: H
authors.lastname: Smet
authors.forename: P
authors.initials: P
authors.lastname: Piot
mesh_terms: D000349:Africa; D000362:Agar; D002876:Chromosomes, Bacterial; D003906:Developing Countries; D004352:Drug Resistance, Microbial / Q000235:genetics; D006069:Gonorrhea / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D010957:Plasmids / Q000235:genetics; D011934:Reagent Strips
chemical_list: D011934:Reagent Strips; D000362:Agar