title: Analysis of a Mycoplasma hominis membrane protein, P120.
abstract: The monoclonal antibody mAb 26.7D generated against a clinical isolate of Mycoplasma hominis 7488 was shown to react with a surface-exposed epitope on a 120-kDa protein (P120). The gene encoding the protein was cloned and sequenced, and the transcriptional start point was determined by primer extension analysis. The gene contained an open reading frame of 3237 bp encoding a peptide of 1079 amino acids with a deduced molecular mass of 123 kDa. A putative amino-terminal signal peptide and cleavage site for signal peptidase II were found. This suggests that the protein was synthesized as a precursor with subsequent processing to a mature lipoprotein. Surface exposure was confirmed by immunoelectron microscopy. mAb 26.7D reacted with 11 of 19 M. hominis strains. The gene was, however, present in all strains.
authors.affiliation: Institute of Medical Microbiology, University of Aarhus, Denmark.
authors.forename: G
authors.initials: G
authors.lastname: Christiansen
authors.forename: S L
authors.initials: SL
authors.lastname: Mathiesen
authors.forename: C
authors.initials: C
authors.lastname: Nyvold
authors.forename: S
authors.initials: S
authors.lastname: Birkelund
mesh_terms: D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D015698:Genomic Library; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D008565:Membrane Proteins; C089295:P120 protein, Mycoplasma hominis