Differences in the hybridization pattern of Bacillus subtilis genes coding for rDNA depend on the method of DNA preparation.
Three different methods of DNA isolation (organic deproteinization, potassium acetate deproteinization, and the use of cetyltrimethylammonium bromide) have been used to prepare DNA from Bacillus subtilis. Subsequent hybridization with an rDNA probe (DNA coding for rRNA) produces different patterns, which mirror those previously reported to indicate an rDNA deletion.
Waterhouse
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Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, Scotland.
Glover
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D000085:Acetates; D019342:Acetic Acid; D001412:Bacillus subtilis / Q000235:genetics* / Q000302:isolation & purification; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification*; D003902:Detergents; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D000085:Acetates; D002593:Cetrimonium Compounds; D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D003902:Detergents; D019342:Acetic Acid; D000077286:Cetrimonium