Cooperation between LH-RH and LH in the direct action on the ovary: LH-RH stimulation of LH/hCG receptors, basal and LH-induced cAMP and cGMP release by porcine granulosa cells in vitro.
LH-RH effects on LH/hCG receptors content, on basal and LH-stimulated cAMP and cGMP release by cultured porcine granulosa cells, were investigated by radioreceptor analysis and radioimmunoassay. It was found that LH-RH additions (10, 100, 1000 or 10,000 ng/ml medium) increased the number of LH/hCG binding sites in granulosa cells. LH or hCG (0.1, 1, 10 or 100 ng/ml) increased both cAMP and cGMP secretion by the cell culture. LH-RH alone (0.1, 1, 10, 100 or 1000 ng/ml) had the same stimulating effect on both cyclic nucleotides. The addition of LH-RH (100 ng/ml) to the medium supplemented with LH (10, 100, 1000, 10,000 or 100,000 ng/ml) enhanced LH-stimulated cAMP and cGMP output from the culture. The present observations suggest that LH-RH can act as synergist of LH and of hCG increasing a number of LH/hCG receptors and stimulating basal and LH-induced cyclic nucleotide release by ovarian cells.
Sirotkin
A V
AV
Research Institute of Animal Production, Nitra, Slovakia.
Nitray
J
J
Kolena
J
J
Bulla
J
J
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000502:physiology*; D006107:Granulosa Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000502:physiology*; D008297:Male; D010053:Ovary / Q000378:metabolism; D011863:Radioimmunoassay; D011869:Radioligand Assay; D011974:Receptors, LH / Q000378:metabolism*; D013552:Swine
D011974:Receptors, LH; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D000242:Cyclic AMP; D006152:Cyclic GMP