title: Three-dimensional growth of glial cell lines affects growth factor and growth factor receptor mRNA levels.
abstract: We have studied, at the mRNA level, the influence of various defined growth conditions on the expression of TGF-alpha, PDGF-BB, EGF-R, PDGF-R alpha, and PDGF-R beta in five different glioma cell lines (D-37MG, D-54MG, D-263MG, GaMG, and U-251MG). RNA isolated from logarithmically growing, confluent monolayer cells or multicellular spheroids was analyzed. Northern blot experiments show that with a few exceptions, specific mRNA steady-state levels were considerably higher in cells grown in a three-dimensional organization relative to cells in the logarithmic growth phase.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bergen, Norway.
authors.forename: G O
authors.initials: GO
authors.lastname: Ness
authors.forename: P H
authors.initials: PH
authors.lastname: Pedersen
authors.forename: R
authors.initials: R
authors.lastname: Bjerkvig
authors.forename: O D
authors.initials: OD
authors.lastname: Laerum
authors.forename: J R
authors.initials: JR
authors.lastname: Lillehaug
mesh_terms: D005910:Glioma / Q000378:metabolism; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D009457:Neuroglia / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D017978:Receptors, Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D006133:Growth Substances; D012333:RNA, Messenger; D017978:Receptors, Growth Factor