title: Application of the RAPD technique in tilapia fish: species and subspecies identification.
abstract: Random Amplified Polymorphic DNA (RAPD) analysis was applied to three species of the tilapia genus Oreochromis and four subspecies of O. niloticus. Thirteen random 10-mer primers were used to assay polymorphisms within and between populations. Different RAPD fragment patterns were observed for different species, although not always for different subspecies. Evidence is presented that RAPD markers might be useful for systemic investigation at the level of species and subspecies.
authors.affiliation: Molecular Biology Research Group, School of Biological Sciences, University of Wales, Swansea, U.K.
authors.forename: F
authors.initials: F
authors.lastname: Bardakci
authors.forename: D O
authors.initials: DO
authors.lastname: Skibinski
mesh_terms: D000818:Animals; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005260:Female; D005819:Genetic Markers; D014644:Genetic Variation / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics*; D017210:Tilapia / Q000145:classification* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers