[Studies on the isolation of endogenous sleep factors from Tupaia Belangeri Chinensis (TBC) after sleep deprivation].
The present study was undertaken to explore endogenous sleep factors isolated from 48-72 h sleep deprived (SD) male Tupaia belangeri chinensis (TBC). Only drink ad libitum (10% glucose) was available within 24 h before collection of urine. Controlled "clean" urinary samples were pooled and stored at 20 C. Fraction I-V from the urine were determined after ultrafiltration and Sephadex-G15. Amino-acid analysis of each fraction was automatically done by a 835 Amino-acid Analyzer, respectively. Bioassay was performed in 40 adult rabbits weighing 2.5-3.5 kg of either sex. Experiments were undertaken via the mesodiencephalic intraventricular infusion. Results show that S2C (Fraction-III) (50 micrograms/rabbit, i.c.v.) exhibited significant delta-enhancing effect compared to the controls. Further purification was done with Sephadex G-25 and Sephadex LH-20. The more purified S4B (50 micrograms/rabbit, i.c.v.) also exhibited significant delta-enhancing effect compared to the controls. The amino-acid analysis of Fraction-III revealed that the compositional contents of S2C and S4B are different from what have been known with Factor S, DSIP and SPS.
Liu
S Y
SY
Shanghai Institute of Organic Chemistry, Academia Sinica.
Zhang
W Y
WY
Li
H Y
HY
Dai
X J
XJ
Zhang
Y
Y
Xu
J C
JC
Chen
L L
LL
Wang
S Q
SQ
Qian
C H
CH
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine; D000818:Animals; D001681:Biological Assay; D005260:Female; D008297:Male; D011817:Rabbits; D012892:Sleep Deprivation; D014417:Tupaia
D000119:Acetylmuramyl-Alanyl-Isoglutamine; C033697:sleep-promoting factor S