Presence and coexistence of putative neurotransmitters in carotid sinus baro- and chemoreceptor afferent neurons.
The presence and coexistence of tyrosine hydroxylase (TH), vasoactive intestinal polypeptide (VIP), calcitonin gene-related peptide (CGRP), substance P (SP) and galanin (GAL) were studied in the petrosal and jugular neurons innervating the carotid body and carotid sinus of the rat. The retrograde labeling of the carotid sinus nerve with Fluoro-gold (FG) demonstrated that most (94.5%) FG-labeled ganglionic neurons were observed in the petrosal ganglion. Fewer (5.2%) FG-labeled neurons were seen in the jugular ganglion and very few (0.3%) were observed in the nodose ganglion. Immunohistochemistry revealed that subpopulations of TH-, VIP-, CGRP-, SP- and GAL-immunoreactive (-ir) neurons in the petrosal ganglion projected to the carotid sinus nerve. Approximately 4% of FG-labeled neurons contained TH-ir and were predominantly found in the caudal portion of the petrosal ganglion. Nearly 90% of total TH-ir neurons in the petrosal ganglion were labeled with FG. Less than 1% of FG-labeled neurons were immunoreactive for VIP in this ganglion. In the petrosal ganglion, 25% of FG-labeled neurons contained CGRP-ir, and 16.7% of FG-labeled neurons contained SP-ir. 30% of CGRP-ir or SP-ir neurons in the petrosal ganglion were labeled with FG. In the jugular ganglion, no TH- or VIP-ir neurons projected to the carotid sinus nerve and only small populations of CGRP- or SP-ir neurons projected to the carotid sinus nerve. Many FG-labeled and GAL-ir neurons were observed in the petrosal and jugular ganglia.(ABSTRACT TRUNCATED AT 250 WORDS)
Ichikawa
H
H
Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
Rabchevsky
A
A
Helke
C J
CJ
D000818:Animals; D002344:Carotid Body / Q000737:chemistry; D002346:Carotid Sinus / Q000294:innervation*; D002628:Chemoreceptor Cells / Q000737:chemistry*; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D008297:Male; D009475:Neurons, Afferent / Q000737:chemistry*; D009479:Neuropeptides / Q000032:analysis; D018377:Neurotransmitter Agents / Q000032:analysis*; D011311:Pressoreceptors / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013266:Stilbamidines; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D013266:Stilbamidines; D014446:Tyrosine 3-Monooxygenase