[The peroxidation of human glycosylated low-density lipoproteins is mediated by the superoxide radical: the protective effects of superoxide dismutase].
Low-density lipoproteins (LDL) oxidized by oxygen radicals are a potent atherogenic stimulus. Chemically modified LDL are internalized by macrophages via a specific cell surface receptor that was termed the scavenger receptor, and could induce foam cell transformation. Post-translational nonenzymatic glycosylation of low density lipoprotein (LDL) occurs in vivo in diabetic patients. Glycosylated LDL (glcLDL) is degraded by macrophages in part by the classic LDL-receptor and in part by the scavenger receptor. This latter mechanism may contribute to the formation of foam cells and acceleration of atherosclerosis in diabetes mellitus. Oxygen free radicals (ORs) could induce LDL peroxidation and subsequent formation of foam cells. Glycosylation may alter protein conformation. A free radical is any chemical species that has an unpaired electron. This property renders it highly chemically reactive. When a radical reacts with a non radical another free radical is generated. This characteristic enables radicals to trigger chain reactions. Oxygen radicals are: superoxide anion (.O2-), hydroxyl radical (.OH) and hydrogen peroxide (H2O2). Thus, the aim of this study was to investigate whether glcLDL are susceptible to peroxidative modification by ORs. GlcLDL was prepared incubating LDL with 40 mM glucose in sterile phosphate-buffer-EDTA 1 mM for 10 days at 37 degrees C. Control LDL (cLDL) was similarly incubated with buffer but without glucose. After this preparation both forms of LDL were oxidized by CuSO4 (15 microM for 20 hours at 37 degrees C) or by xanthine/xanthine oxidase (X:2 mM/XO: 100 mU for 20 hours at 37 degrees C).(ABSTRACT TRUNCATED AT 250 WORDS)
Napoli
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Cattedra di Cardiologia, II Facoltà di Medicina e Chirurgia, Università degli Studi Federico II, Napoli.
Ambrosio
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Palumbo
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Chiariello
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Duilio
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Chiariello
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D003920:Diabetes Mellitus / Q000097:blood; D004587:Electrophoresis, Agar Gel; D016166:Free Radical Scavengers; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood* / Q000302:isolation & purification; D008315:Malondialdehyde / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood*; D013481:Superoxides / Q000097:blood*
D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D013481:Superoxides; D008315:Malondialdehyde; D013482:Superoxide Dismutase
La perossidazione delle lipoproteine glicosilate umane a bassa densità è mediata dal radicale superossido: effetti protettivi della superossido dismutasi.