Differentiation of HL-60 cells is promoted by H-toxin of Clostridium septicum.
H-toxin of Clostridium septicum potentiated dimethyl sulfoxide (DMSO)-induced differentiation of human promyelocytic leukemia HL-60 cells which were monitored by nuclear morphology and production of oxidative radicals. But, H-toxin did not induce differentiation of HL-60 cells in the absence of DMSO. These phenomena were not observed by staphylococcal leukocidin, a cytotoxin affecting to HL-60 cells. In HL-60 cells, ADP-ribosylation of 118, 93, 75 and 58 kDa membrane proteins was observed, but the ADP-ribosylation was not detected either in differentiated HL-60 cells by DMSO or in normal polymorphonuclear leukocytes of human. When the membranes of HL-60 cells were incubated with H-toxin, ADP-ribosylation of the membrane proteins was inhibited. Such suppressive effects on ADP-ribosylation were not observed by DMSO or staphylococcal leukocidin. These data suggest that inhibition of the ADP-ribosylation by H-toxin may play an important role in potentiation of DMSO-induced differentiation of HL-60 cells.
Morinaga
N
N
Second Department of Microbiology, School of Medicine, Chiba University, Japan.
Kato
I
I
Noda
M
M
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D001427:Bacterial Toxins / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003013:Clostridium; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006801:Humans; D014407:Tumor Cells, Cultured
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C064270:hemolytic toxin, Clostridium septicum; D000246:Adenosine Diphosphate Ribose; D004121:Dimethyl Sulfoxide