PI-3-kinase inhibitor Wortmannin blocks the insulin-like effects of growth hormone in isolated rat adipocytes.
The effect of wortmannin, a selective phosphatidylinositol 3-kinase inhibitor, on the insulin-like effects of growth hormone in isolated adipocytes from rat was investigated. Wortmannin inhibited both the lipogenic and the antilipolytic effects (IC50 approximately 20 nM) with no effect on [125I]-growth hormone binding to the adipocytes. These data suggest that phosphatidylinositol 3-kinase might play an important role in the insulin-like actions of growth hormone.
Ridderstråle
M
M
Department of Paediatrics, University of Lund, Sweden.
Tornqvist
H
H
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000730:Androstadienes / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D013006:Growth Hormone / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008066:Lipolysis / Q000187:drug effects*; D019869:Phosphatidylinositol 3-Kinases; D010739:Phospholipase D / Q000037:antagonists & inhibitors*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011986:Receptors, Somatotropin / Q000378:metabolism*; D014280:Triglycerides / Q000096:biosynthesis*; D000077191:Wortmannin
D000730:Androstadienes; D007328:Insulin; D011986:Receptors, Somatotropin; D014280:Triglycerides; D013006:Growth Hormone; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D010739:Phospholipase D; D005947:Glucose; D000077191:Wortmannin