[The evaluation of the effect of formol and sodium hypochlorite on the demonstration of Cryptosporidium oocysts in feces by Heine's method].
Cryptosporidium oocysts were searched by Heine's method in stools of nine calves with cryptosporidiosis after stool treatment with two disinfectants, 10% paraformaldehyde solution and 14.5% sodium hypochlorite solution. After 30 minutes exposition to sodium hypochlorite solution oocysts became non refractile and acquired a reddish tinge, making their identification difficult. No morphological alterations occurred in oocysts after paraformaldehyde treatment. We recommend paraformaldehyde at 10% concentration as means of human immunodeficiency virus (HIV) inactivation for routine use in stool examinations and therefore making safer those type of procedures for laboratory personnel, when using Heine's method.
Módolo
J R
JR
Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Universidade Estadual Paulista, SP, Brazil.
Amato Neto
V
V
Braz
L M
LM
Lopes
C A
CA
Gottschalk
A F
AF
D017088:AIDS-Related Opportunistic Infections / Q000469:parasitology; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D003457:Cryptosporidiosis / Q000469:parasitology; D003458:Cryptosporidium / Q000187:drug effects* / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology*; D005557:Formaldehyde / Q000494:pharmacology*; D015497:HIV-1; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods; D012973:Sodium Hypochlorite / Q000494:pharmacology*
D007202:Indicators and Reagents; D005557:Formaldehyde; D012973:Sodium Hypochlorite
Avaliação da influência do formol e do hipoclorito de sódio na pesquisa de oocistos de Cryptosporidium nas fezes, através do método de heine.