title: Characterization of four new gastric cancer cell lines.
abstract: Four well differentiated gastric adenocarcinoma cell lines from German patients have been established from primary tumors (St 23132, St 3051) and lymph node metastases (St 2474, St 2957). The tumor cells were isolated by enzymatic or mechanical treatment. All four lines grew as solid tumors in nude mice and formed colonies in soft agar. The doubling time of the cells in culture was 25-32 h. Further characteristics of the lines were a considerable chromosomal aneuploidy, (the chromosomal numbers varying from 30-109 with many numerical and structural abnormalities), a stable keratin expression (Ck 8, 18, 19), the expression and secretion of CEA and CA-19-9 and the overexpression of c-myc. The four stomach cancer cell lines described here are not only a useful addition to the small number of existing lines, but also represent ideal tools for studying tumorigenicity of human stomach cancers in vitro and in vivo.
authors.affiliation: Institut für Pathologie, Würzburg, Germany.
authors.forename: H P
authors.initials: HP
authors.lastname: Vollmers
authors.forename: K
authors.initials: K
authors.lastname: Stulle
authors.forename: J
authors.initials: J
authors.lastname: Dämmrich
authors.forename: M
authors.initials: M
authors.lastname: Pfaff
authors.forename: T
authors.initials: T
authors.lastname: Papadopoulos
authors.forename: C
authors.initials: C
authors.lastname: Betz
authors.forename: K
authors.initials: K
authors.lastname: Saal
authors.forename: H K
authors.initials: HK
authors.lastname: Müller-Hermelink
mesh_terms: D000782:Aneuploidy; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002455:Cell Division; D002469:Cell Separation; D002869:Chromosome Aberrations; D015870:Gene Expression; D016259:Genes, myc; D005858:Germany; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008207:Lymphatic Metastasis; D051379:Mice; D008819:Mice, Nude; D010641:Phenotype; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D002272:Carcinoembryonic Antigen