Induction of DNA fragmentation in human myelogenous leukemic cell lines by sodium 5,6-benzylidene-L-ascorbate and its related compounds.
High-performance liquid chromatography revealed that sodium 5,6-benzylidene-L-ascorbate (SBA), dissolved in distilled water, was gradually decomposed into ascorbic acid and benzaldehyde. Among these three compounds, ascorbic acid showed the most potent cytotoxic activity. The cytotoxic activity of each compound was significantly reduced during degradation in culture medium. Agarose gel electrophoresis and fluorometric determination of DNA revealed that ascorbic acid, as well as SBA, induced DNA fragmentation into nucleosomal oligomers in human myelogenous leukemic cell lines, but not in freshly isolated human peripheral blood cells. The results suggest that antitumor activity of SBA might be at least in part mediated by the action of ascorbic acid, a degradation product of SBA.
Kuribayashi
N
N
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Sakagami
H
H
Sakagami
T
T
Niimi
E
E
Shiokawa
D
D
Ikekita
M
M
Takeda
M
M
Tanuma
S
S
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D001547:Benzaldehydes / Q000378:metabolism; D001597:Benzylidene Compounds / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology; D051379:Mice
D000970:Antineoplastic Agents; D001547:Benzaldehydes; D001597:Benzylidene Compounds; D004247:DNA; D001205:Ascorbic Acid; C070680:zilascorb; C032175:benzaldehyde