13-HODE inhibits the intracellular calcium increase of activated human polymorphonuclear cells.
We studied the effect of the linoleic acid metabolite 13-hydroxyoctadecadienoic acid (13-HODE) on the increase of the intracellular calcium concentration, [Ca2+]i, induced by platelet-activating factor (PAF), leukotriene B4 (LTB4), and formyl-Met-Leu-Phe (fMLP) in human polymorphonuclear leukocytes (PMNs). 13-HODE by itself did not change the [Ca2+]i of PMNs. However, when PMNs were preincubated with 13-HODE and subsequently stimulated with PAF, LTB4, or fMLP, an inhibition of the increase of the [Ca2+]i was observed. The PAF-induced calcium response was concentration-dependently inhibited between 0.1 and 5 microM 13-HODE. These results suggest that 13-HODE can affect the increase of the [Ca2+]i and hence can modulate activation of PMNs.
van de Velde
M J
MJ
Department of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands.
Engels
F
F
Henricks
P A
PA
Nijkamp
F P
FP
D000595:Amino Acid Sequence; D002118:Calcium / Q000097:blood*; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D007975:Leukotriene B4 / Q000494:pharmacology; D008041:Linoleic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology; D013268:Stimulation, Chemical
D008041:Linoleic Acids; D010972:Platelet Activating Factor; D007975:Leukotriene B4; C024348:13-hydroxy-9,11-octadecadienoic acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002118:Calcium