Method for simultaneous determination of the distribution of capillary plasma flow and myocyte redox state (NADH-fluorescence) in the hypoperfused myocardium of the anesthetized rat.
A method is described which allows to observe the pattern of capillary plasma filling simultaneously with the redox state (NADH-fluorescence) of the myocytes in the hypoperfused myocardium. In anesthetized rats the coronary perfusion pressure was reduced to a defined level, the blood plasma labeled with dye-conjugated albumin and a myocardial biopsy-sampled. Freeze-substitution of histological sections allowed to detect the intravascular plasma label as well as the myocyte NADH-fluorescence on a microscopic level. It was found that areas showing increased cellular NADH-fluorescence did not arise in a distribution congruent with zones of failing capillary plasma flow in the hypoperfused myocardium.
Vetterlein
F
F
Zentrum Pharmakologie und Toxikologie, Universität, Göttingen.
Prange
M
M
Tran
P N
PN
Schmidt
G
G
D000758:Anesthesia; D000818:Animals; D002196:Capillaries / Q000502:physiology; D003326:Coronary Circulation / Q000502:physiology*; D003331:Coronary Vessels / Q000502:physiology; D005453:Fluorescence; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats; D012039:Regional Blood Flow / Q000502:physiology
D009243:NAD