Protection studies with integral membrane fractions of Haemonchus contortus.
Techniques for targetting glycoproteins integral to the luminal membrane of the intestinal cells of Haemonchus contortus were used to isolate fractions of whole parasites with protective antigen potential. Sheep immunization trials with various candidate fractions revealed one which selectively bound to lectins with specificity for N-acetylgalactosamine and which reduced mean challenge worm burdens by up to 72% and mean faecal egg counts by up to 93%. The luminal surface of the intestines of the Haemonchus recovered from sheep immunized with this antigen were coated with host immunoglobulin, suggesting that the protective effect was due to antibodies interfering with the function of the gut. Further biochemical characterization of this fraction, which has been termed Haemonchus galactose-containing glycoprotein complex (H-gal-GP complex), showed that it could be distinguished from previously described protective antigens of this parasite and that it was only detectable in detergent extracts of the worms.
Smith
W D
WD
Moredun Research Institute, Edinburgh, Scotland, UK.
Smith
S K
SK
Murray
J M
JM
D000116:Acetylgalactosamine; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D010450:Endopeptidases / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D006188:Haemonchiasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D015801:Helminth Proteins / Q000276:immunology* / Q000302:isolation & purification; D007422:Intestines / Q000737:chemistry / Q000276:immunology; D037102:Lectins; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000517:prevention & control*
D000909:Antibodies, Helminth; D015801:Helminth Proteins; D037102:Lectins; D008562:Membrane Glycoproteins; D010450:Endopeptidases; C119206:galactose-containing glycoprotein, Haemonchus contortus; D000116:Acetylgalactosamine