Interdomain linkage in the polymeric hemoglobin molecule of Artemia.
Artemia has evolved the longest known concatenation of hemoglobin domains, the alpha subunit containing nine domains and the beta subunit having a similar size. Translation of the cDNA sequence of the alpha subunit reveals eight regions of inter-domain polypeptide linking together the nine heme-binding domains, together with partially analogous sequences preceding the first domain and following the last. Analysis of the structural possibilities of the linker sequences suggests how the domains may be organized in the subunit. The interdomain linker sequences were 14%-64% identical (62%-91% similar by Dayhoff substitution matrix) and approximately 14 residues in length including a consensus -Val-Asp-Pro-Val-Thr-Gly-Leu-. The linker composition resembled that of the 11 amino acid pre-A leader sequence of Petromyzon marinus (lamprey) hemoglobin V, the structure of which is known. Prediction of structure from the Artemia linker sequences indicated a nonhelical, turn-associated linker which could be modeled to the Petromyzon leader. Measurements confirmed that such a structure could support the packing of nine Artemia domains into a polymeric subunit of annular shape, two of which subunits (which can be similar or dissimilar) comprise the physiological molecule.(ABSTRACT TRUNCATED AT 250 WORDS)
Trotman
C N
CN
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Manning
A M
AM
Bray
J A
JA
Jellie
A M
AM
Moens
L
L
Tate
W P
WP
D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D005914:Globins / Q000737:chemistry / Q000235:genetics*; D006454:Hemoglobins / Q000235:genetics*; D007438:Introns; D007798:Lampreys / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D006454:Hemoglobins; D021382:Protein Sorting Signals; D005914:Globins