The identification of a complex family of low-molecular-weight GTP-binding protein homologues from Paramecium tetraurelia by PCR cloning.
We have used the polymerase chain reaction to clone a large number of gene fragments encoding low-molecular-weight GTP-binding proteins (LMW G-proteins) from Paramecium tetraurelia. All clones were subjected to a computer-assisted search of the GenBank databases to assign putative homologues. On the basis of several conserved features, we place these genes in the rab/ypt and rho subfamilies of the LMW G-proteins. Northern blot analyses indicated that all clones were from active genes. One surprising feature of the cloned genomic sequences was the presence of unusually short introns (20-30 nucleotides).
Fraga
D
D
Fred Hutchinson Cancer Research Center, Seattle, WA 98115.
Hinrichsen
R D
RD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010247:Paramecium / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D012333:RNA, Messenger; D016053:RNA, Protozoan; D019204:GTP-Binding Proteins