Superhelical density of goat mitochondrial DNA: fluorimetric studies.
EtBr binding to DNA has been used to determine the superhelical density of Goat mtDNA. The differential binding of closed circular and nicked circular DNA is estimated by simple fluorescence measurements, which is a function of state of DNA supercoiling. The method is simple and quick over the cesium chloride density gradient centrifugation studies.
Chatterjee
B
B
Department of Biochemistry, Banaras Hindu University, Varanasi.
Rao
G R
GR
D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry*; D004278:DNA, Superhelical / Q000737:chemistry*; D005470:Fluorometry; D006041:Goats / Q000235:genetics* / Q000378:metabolism
D004272:DNA, Mitochondrial; D004278:DNA, Superhelical