title: Inhibition of rhodopsin phosphorylation by non-myristoylated recombinant recoverin.
abstract: Bovine recoverin regulates rhodopsin phosphorylation and controls photoreceptor light sensitivity in a Ca(2+)-dependent manner. Recoverin is post-translationally modified with lipids (myristic acid or related lipids) at its N-terminus. Since with this lipid modification (N-myristoylation), recoverin associates with rod outer segment membranes in a Ca(2+)-dependent manner, N-myristoylation has been suggested to be important for the function of this protein. To study the role of this modification, we obtained recombinant non-myristoylated recoverin in E. coli and studied its functional properties. Here, we report that recombinant non-myristoylated recoverin inhibits rhodopsin phosphorylation at Ca2+ concentrations of 30 nM-10 microM in a similar way as native N-myristoylated recoverin does. Thus, our result showed that N-myristoylation is not essential for the Ca(2+)-dependent inhibition of rhodopsin phosphorylation by recoverin.
authors.affiliation: Department of Physiology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kawamura
authors.forename: J A
authors.initials: JA
authors.lastname: Cox
authors.forename: P
authors.initials: P
authors.lastname: Nef
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D005136:Eye Proteins; D051597:Hippocalcin; D008074:Lipoproteins; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011898:Ranidae; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D051603:Recoverin; D012243:Rhodopsin / Q000378:metabolism*; D012374:Rod Cell Outer Segment / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009227:Myristic Acids; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D011994:Recombinant Proteins; D019814:Myristic Acid; D051603:Recoverin; D051597:Hippocalcin; D000255:Adenosine Triphosphate; D012243:Rhodopsin; D002118:Calcium