Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP method.
Echinococcus species and genetically distinct strains of Echinococcus granulosus can be rapidly and reliably identified using a polymerase chain reaction (PCR)-linked restriction fragment length polymorphism (RFLP) method which surveys the sequence of a rapidly evolving region of the ribosomal DNA (rDNA) unit. Internal transcribed spacer 1 (ITS1) of the rDNA repeat was amplified from various isolates and the product was digested with one of a number of 4-base cutting restriction enzymes. Characteristic patterns were produced when samples within various species and strain groups were analysed. This method offers an objective, simple, highly sensitive and rapid approach for the discrimination of Echinococcus isolates and for study of other parasite complexes.
Bowles
J
J
Molecular Helminthology Laboratory, Queensland Institute of Medical Research, Brisbane, Australia.
McManus
D P
DP
D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics; D004446:Echinococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D013045:Species Specificity
D004275:DNA, Ribosomal