title: Respiration of [14C]alanine by the ectomycorrhizal fungus Paxillus involutus.
abstract: The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [14C]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [14C]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed 14CO2 evolution and greatly reduced the flow of carbon from [14C]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.
authors.affiliation: Department of Microbial Ecology, Lund University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Chalot
authors.forename: A
authors.initials: A
authors.lastname: Brun
authors.forename: R D
authors.initials: RD
authors.lastname: Finlay
authors.forename: B
authors.initials: B
authors.lastname: Söderström
mesh_terms: D000363:Agaricales / Q000378:metabolism*; D000409:Alanine / Q000378:metabolism*; D010101:Oxygen Consumption; D013559:Symbiosis
chemical_list: D000409:Alanine