title: Complete amino acid sequence of ribosomal protein S14 from Bacillus stearothermophilus and homology studies to other ribosomal proteins.
abstract: The complete amino acid sequence of protein S14 from the small subunit of Bacillus stearothermophilus was determined by N-terminal sequence analysis and by sequencing of overlapping peptides obtained from enzymatic digestions. Protein S14 consists of 60 amino acid residues with a molecular mass of 7148 Da. It has a high content of basic amino acids and a predicted isoelectric point of 11.46. Protein S14 contains two pairs of cysteines in the carboxyl-terminal region, presumably linked by two sulphur bridges. A comparison between protein S14 of B. stearothermophilus and homologous proteins from other organisms revealed highly conserved carboxyl-termini for this protein in eubacteria, archaebacteria and eukaryotes.
authors.affiliation: Max-Delbrück-Centrum für Molekulare Medizin, Abteilung Proteinchemie, Berlin, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Herfurth
authors.forename: U
authors.initials: U
authors.lastname: Briesemeister
authors.forename: B
authors.initials: B
authors.lastname: Wittmann-Liebold
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D001411:Geobacillus stearothermophilus / Q000737:chemistry*; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D017386:Sequence Homology, Amino Acid
chemical_list: D012269:Ribosomal Proteins; C039747:ribosomal protein S14