Sequencing for 4 single-copy DNA segments from human chromosome 8q24.1.
A successful method for constructing human chromosome 8q24.1 band specific probe pool by microdissection and microcloning is described in this paper. The probe pool was proved to be band-specific by fluorescence in situ hybridization. Through Southern blot analysis, 34 single copy DNA segments were obtained and four of them were sequenced. It is possible to provide useful landmarks for constructing human chromosome 8q24.1 physical map and STSs map.
Xia
J H
JH
State Key Laboratory of Medical Genetics, Human Medical University, Changsha.
Ruan
Q G
QG
He
X X
XX
Li
L L
LL
Huang
L
L
Deng
H X
HX
Li
L Y
LY
D001483:Base Sequence; D015139:Blotting, Southern; D002898:Chromosomes, Human, Pair 8; D004247:DNA / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D004247:DNA