Inhibition of beta-adrenergic binding by fungal metabolites.
Strains of Aspergillus flavus, Fusarium sp., Rhizopus sp. and Candida albicans all produced inhibitors of beta-adrenergic receptor binding; strains of Saccharomyces sp. and Schizosaccharomyces sp. did not. In tests with glutamic acid as the sole nutrient source, a Fusarium sp. produced four-fold larger amounts of inhibitor than the other fungi. The inhibitor from the Fusarium sp. was further purified by lyophilisation and sequential solvent extraction in chloroform, ethyl acetate and butanol; 60% of the original activity was recovered. The inhibitor had an estimated molecular size of 650 Da, and did not absorb light in the visible or ultraviolet range. When compared with a similar inhibitor from Escherichia coli, the Fusarium sp. inhibitor appeared to be a more potent inhibitor of beta-adrenergic and dopaminergic binding to mammalian cells.
Coleman
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WH
Department of Biology, University of Hartford, West Hartford, CT 06117.
Donta
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D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001231:Aspergillus flavus / Q000378:metabolism*; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003470:Culture Media; D004926:Escherichia coli / Q000378:metabolism; D005670:Fusarium / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D051379:Mice; D051381:Rats
D000319:Adrenergic beta-Antagonists; D003470:Culture Media; D005971:Glutamates; D018698:Glutamic Acid