title: The origin and genetic differentiation of native breeds of pigs in southwest China: an approach from mitochondrial DNA polymorphism.
abstract: Mitochondrial DNA (mtDNA) of six breeds of native domestic pigs from Yunnan province, southwest China, and two wild boars obtained from Sichuan, China, and Vietnam was analyzed using 20 restriction endonucleases that recognize six nucleotides. Restriction maps were made by double-digestion methods and polymorphic sites were located on the map. According to their mtDNA restriction types, all the breeds were classified into six groups. Genetic distances among groups were calculated to define their phylogenetic relationships. The relationship between the Sichuan wild boar and domestic pigs is close, while the Vietnamese wild boar is relatively far from them, so the domestic pigs in southwest China are likely to have originated from a wild pig which distributed in west China. We compare our results with previous reports in literature and discuss the relationship among Chinese pigs, Japanese pigs, and European pigs. The mtDNA cleavage pattern of the Mingguang pig digested by EcoRV was identical to that of Duroc; mutations at the EcoRI site, detected in the mtDNA of two Dahe pigs, are the same as in the Vietnamese wild boar, suggesting that mutational hot spots exist in the mtDNA of pigs.
authors.affiliation: Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Science.
authors.forename: H
authors.initials: H
authors.lastname: Lan
authors.forename: L
authors.initials: L
authors.lastname: Shi
mesh_terms: D000818:Animals; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D009154:Mutation; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013552:Swine / Q000145:classification / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial