Lithocholic acid-sensitive mutant cells isolated from Chinese hamster ovary cells are cross-sensitive to cytoskeleton-disturbing agents.
The lithocholic acid-sensitive mutant cells were isolated from CHO-9 cells mutagenized with ethylmethane sulfonate, through clonal selection on 96-well dishes. The isolated mutant cells (LSJ-11) were 3-fold more sensitive than controls in the survival to lithocholic acid and were also sensitive to other bile acids such as deoxycholic acid and cholic acid. As a preliminary characterization, it was found that the mutant cells were also sensitive to cytoskeleton-disturbing agents such as colchicine, colcemide, taxol and cytochalasin B. This is the first observation that a bile acid-sensitive mutant was isolated and was partially characterized.
Kaneko
M
M
Biophysics Division, National Cancer Center Research Institute, Tokyo, Japan.
Kodama
M
M
Inoue
F
F
Horikoshi
J
J
D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects*; D003078:Colchicine / Q000633:toxicity; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D003571:Cytochalasin B / Q000633:toxicity; D003599:Cytoskeleton / Q000187:drug effects*; D003703:Demecolcine / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D008095:Lithocholic Acid / Q000378:metabolism / Q000633:toxicity*; D016296:Mutagenesis; D009154:Mutation; D017239:Paclitaxel / Q000633:toxicity
D003571:Cytochalasin B; D008095:Lithocholic Acid; D005020:Ethyl Methanesulfonate; D017239:Paclitaxel; D003078:Colchicine; D003703:Demecolcine