Restriction map of the genomic DNA of Lactobacillus casei bacteriophage PL-1 and nucleotide sequence of its cohesive single-stranded ends.
A restriction map of the genomic DNA of Lactobacillus casei phage PL-1 was constructed using the restriction endonucleases BamHI, EcoRI, HindIII, KpnI, NruI and XhoI. The PL-1 genome was 42.2 kb in size and had complementary cohesive ends forming a ring-like monomer. The cohesive ends, analysed with exonuclease III and S1 nuclease, were 3'-terminated single strands protruding from both ends. The nucleotide sequence of the cohesive ends, determined by the dideoxynucleotide method, comprised four A + T and 10 G + C pairs: 5' GAGGCCGACCGTTC 3'/3' CTCCGGCTGGCAAG 5'. Thus, the cohesive ends of PL-1 DNA were higher in G + C content than those of other known bacteriophage DNAs.
Nakashima
Y
Y
Microbiology Laboratory, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
Ikeda
H
H
Kakita
Y
Y
Miake
F
F
Watanabe
K
K
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D016679:Genome, Viral; D007780:Lacticaseibacillus casei; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004262:DNA Restriction Enzymes