Identification of a unique group of human papillomavirus type 16 sequence variants among clinical isolates from Barbados.
The naturally occurring sequence variation of human papillomavirus type 16 (HPV-16) was analysed by direct sequence analysis of the PCR products of the long control region (LCR), the E5 and E7 open reading frames (ORFs), a segment of the L2 ORF overlapping the early viral poly(A) signal and a small segment of the L1 ORF or clinical isolates from Barbados and The Netherlands. Despite the widely different geographical and ethnic origin of the two groups of specimens, sequence analysis revealed relatively few mutational differences. Analysis of the LCR and the E5 ORF appeared to be the minimum requirement for the correct positioning of these variants in the HPV-16 phylogenetic tree. Most of the Barbadian variants appeared to be located at a unique position in the HPV-16 phylogenetic tree, at the internal branch close to the point where the European and Asian branches diverge. In contrast, most of the Dutch samples were located on the European branch.
Smits
H L
HL
Department of Virology, Academic Medical Centre, University of Amsterdam, The Netherlands.
Traanberg
K F
KF
Krul
M R
MR
Prussia
P R
PR
Kuiken
C L
CL
Jebbink
M F
MF
Kleyne
J A
JA
van den Berg
R H
RH
Capone
B
B
de Bruyn
A
A
D001208:Asia; D001460:Barbados; D001483:Base Sequence; D017931:DNA Primers; D005006:Ethnicity; D005060:Europe; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009426:Netherlands; D009856:Oncogene Proteins, Viral / Q000235:genetics; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification*; D050725:Papillomavirus E7 Proteins; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D000086082:Viroporin Proteins
D017931:DNA Primers; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C054859:oncogene protein E5, Human papillomavirus type 16; D000086082:Viroporin Proteins; C059731:oncogene protein E7, Human papillomavirus type 16