title: Validation of the species concept in the genus Dekkera by restriction analysis of genes coding for rRNA.
abstract: The gene coding for the small-subunit rRNA of 11 type strains belonging to the genus Dekkera and its anamorph, Brettanomyces, was amplified by using the polymerase chain reaction and subjected to digestion with a series of restriction endonucleases. Similarity coefficients were calculated from the number of shared and unique fragments, and a cluster analysis yielded four distinct groups with the following ascosporogenous states: Dekkera anomala, Dekkera bruxellensis, Dekkera custersiana, and Dekkera naardenensis. Results correlate with evidence from isoenzyme electrophoresis and DNA homology analysis. They also confirm previously reported anamorph-teleomorph connections and recently proposed synonymies within the genus.
authors.affiliation: American Type Culture Collection, Rockville, Maryland 20852.
authors.forename: F I
authors.initials: FI
authors.lastname: Molina
authors.forename: P
authors.initials: P
authors.lastname: Shen
authors.forename: S C
authors.initials: SC
authors.lastname: Jong
mesh_terms: D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D015003:Yeasts / Q000145:classification* / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D012335:RNA, Ribosomal