An Escherichia coli cyoE gene homologue in thermophilic Bacillus PS3 encodes a thermotolerant heme O synthase.
The cyoE gene of the Escherichia coli bo-type quinol oxidase operon (cyoABCDE) has been previously shown to encode heme O synthase. To demonstrate a catalytic role of a cyoE homologue (the caaE gene) in the gene cluster for caa3-type cytochrome c oxidase of thermophilic Bacillus PS3, we have carried out genetic complementation analysis using the chimeric operon cyoABCD-caaE and heme O synthase assay using the CaaE-overproduced E. coli membranes. We found that the caaE gene encodes a thermotolerant heme O synthase which provides an intermediate for heme A biosynthesis.
Saiki
K
K
Department of Plant Sciences, Graduate School of Science, University of Tokyo, Japan.
Mogi
T
T
Ishizuka
M
M
Anraku
Y
Y
D019883:Alkyl and Aryl Transferases; D001407:Bacillus / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002384:Catalysis; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006418:Heme / Q000378:metabolism; D006358:Hot Temperature; D008969:Molecular Sequence Data; D014166:Transferases / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C069808:heme O; D006418:Heme; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C078356:CyoE protein, Bacteria; C489727:CyoE protein, E coli