Immunophenotyping of murine peritoneal macrophages and lymphocytes by flow cytometry.
Peritoneal cells include mainly macrophages and both T and B lymphocytes. We describe a simple procedure to analyze by flow cytometry the antigens expressed on macrophage and/or lymphocyte membranes. F4/80 and CR3 macrophage antigens were detected with anti-F4/80 and anti-Mac-1 monoclonal antibodies (mAb) respectively, CD4 and THY1.2 T lymphocyte antigens with GK1.5 and J1j mAbs respectively, B CD5 lymphocytes were identified with LY1.2 mAb and Fc gamma RII/Fc gamma RIII were detected with 2.4G2 mAb.
Plasman
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N
Laboratoire d'Immunologie, Faculté de Médecine, Université Libre de Bruxelles, Belgium.
Vray
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D005434:Flow Cytometry; D016130:Immunophenotyping; D008214:Lymphocytes / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface