A panel of radiation hybrids for human chromosome 8.
We have developed a panel of radiation hybrids containing fragments of chromosome 8 as the only human material. The human chromosome content of each cell line was determined relative to an ordered map of sequence tagged sites (STSs) specific to chromosome 8. Between one and four fragments of chromosome 8 were identified in each cell line, with an average of 25% of the STSs retained in each line. Subclones of one radiation hybrid were examined to determine whether all cells within a line are homogeneous with respect to chromosome 8 sequence content. There was considerable variability between subclones, with retention rates for individual STSs ranging from 5 to 100% in different clones. Furthermore, a gradient of retention of sequences along the length of one large chromosome fragment was found, suggesting that sequence loss involved deletions from one end of the fragment at early stages in the establishment of the cell line. We have also made use of the radiation hybrids to develop novel sequence tagged sites for the pericentromeric region of chromosome 8.
Sapru
M
M
Department of Biology, University of Houston, Texas 77204.
Gu
J
J
Gu
X
X
Smith
D
D
Yu
C E
CE
Wells
D
D
Wagner
M
M
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods; D002898:Chromosomes, Human, Pair 8 / Q000528:radiation effects / Q000648:ultrastructure; D006224:Cricetinae; D017931:DNA Primers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012641:Selection, Genetic; D016324:Sequence Tagged Sites
D017931:DNA Primers; D000228:Adenine Phosphoribosyltransferase