Loss of flagellation in dnaA mutants of Escherichia coli.
A dnaA46 mutant of Escherichia coli showed loss of motility at 37 degrees C, a permissive temperature for cell growth of this mutant. Other dnaA mutations near the middle of the gene also caused an immotile phenotype. The amount of flagellin was much less in the dnaA46 mutant than in the wild-type control, as was the promoter activity. DnaA protein may play an important role in expression of the fliC gene.
Mizushima
T
T
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Tomura
A
A
Shinpuku
T
T
Miki
T
T
Sekimizu
K
K
D001426:Bacterial Proteins / Q000235:genetics*; D002455:Cell Division; D002465:Cell Movement; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005407:Flagella / Q000502:physiology*; D009154:Mutation; D013696:Temperature
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria