Energetics of cyanophage N-1 multiplication in the diazotrophic cyanobacterium Nostoc muscorum.
Cyanophage N-1 multiplication was investigated during the latent period of the virus, when 14CO2 fixation was inhibited whereas respiratory O2 uptake increased approximately 67% at 4 h after infection. A simultaneous decrease (70%) in the glycogen content of infected cells indicated its catabolic involvement. A chloramphenicol-sensitive rise in glucose-6-phosphate dehydrogenase activity as a result of N-1 infection partly explained the increase in aerobic respiration. The total ATP pool declined to 53% of the control while Ca(2+)-dependent ATPase activity also declined (25%). In contrast, Mg(2+)-dependent ATPase activity increased (80%) in comparison with uninfected cells. Results suggest that oxidative phosphorylation was more crucial in the control of cyanophage N-1 development than photophosphorylation under photoautotrophic growth conditions.
Singh
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S
Department of Biological Sciences, Rani Durgavati University, Jabalpur, India.
Bhatnagar
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A
Kashyap
A K
AK
D000251:Adenosine Triphosphatases / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D001435:Bacteriophages / Q000254:growth & development*; D002245:Carbon Dioxide / Q000378:metabolism; D000458:Cyanobacteria / Q000502:physiology*; D004734:Energy Metabolism; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006003:Glycogen / Q000032:analysis; D010101:Oxygen Consumption
D002245:Carbon Dioxide; D000255:Adenosine Triphosphate; D006003:Glycogen; D005954:Glucosephosphate Dehydrogenase; D000251:Adenosine Triphosphatases