Identification and nucleotide sequence of Brucella melitensis L7/L12 ribosomal protein.
DNA sequencing of the gene encoding a Brucella melitensis 12-kDa protein revealed that this protein was the ribosomal protein L7/L12. The B. melitensis L7/L12 DNA sequence was identical to that of the corresponding B. abortus gene, showing the near identity of these two organisms. When comparing the sequence of this protein to that of other organisms some domains were highly conserved, especially the C-terminus, which contrasted with the lack of conservation of the sequences at the N-terminus. The finding that the ribosomal protein L7/L12 of Brucella is an immunodominant antigen provides a new rationale to explain the activity of ribosomal vaccines.
Bachrach
G
G
Department of Clinical Microbiology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Bar-Nir
D
D
Banai
M
M
Bercovier
H
H
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D017347:Brucella melitensis / Q000235:genetics*; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12