Composition of peritoneal macrophage membranes in autoimmune MRL lpr/lpr mice.
Adult MRL lpr/lpr mice display phenotypic features that are consistent with both rheumatoid arthritis and systemic lupus erythematosus. Previous studies have reported that peritoneal macrophages harvested from this model have an increased propensity for both spontaneous and elicited release of prostaglandins and leukotrienes relative to immunologically normal control mice. To investigate whether one aspect of the differences in secretory potential between autoimmune and normal mice was at the level of increased substrate availability, gas chromatographic analysis of peritoneal macrophage membranes from autoimmune MRL lpr/lpr, young lpr, wild type +/+, and immunologically normal mice was done. The results demonstrate enrichment of arachidonate in adult lpr macrophage membranes in all major phospholipid classes relative to young lpr, +/+ and immunologically normal C3H/HeN mice. Similarly, there was an increased mole % of arachidonic acid in lpr mice relative to controls. Elevated membrane arachidonate may contribute to the increased propensity of autoimmune strains to participate in the inflammatory process.
Hackshaw
K V
KV
Department of Internal Medicine, William H. Davis Medical Research Center, Ohio State University, Columbus 43210.
Jackson
N A
NA
Shi
Y
Y
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology*; D002462:Cell Membrane / Q000737:chemistry; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D017737:Macrophages, Peritoneal / Q000737:chemistry*; D008563:Membrane Lipids / Q000032:analysis*; D051379:Mice; D008809:Mice, Inbred C3H; D008817:Mice, Mutant Strains; D010743:Phospholipids / Q000032:analysis*; D012016:Reference Values; D013045:Species Specificity
D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids