title: Allelic inactivation regulates olfactory receptor gene expression.
abstract: We suggest a model in which a hierarchy of controls is exerted on the family of odorant receptor genes to assure that a sensory neuron expresses a single receptor from a family of 1000 genes. We propose that a cis-regulatory element directs the stochastic expression of only one gene from a large array of linked receptor genes. Moreover, only one allelic array encoding multiple receptor genes is active in an individual neuron. We demonstrate that in a neuron expressing a given receptor, expression derives exclusively from one allele. In addition, we observe that alleles encoding the odorant receptors are replicated asynchronously, a phenomenon consistently associated with allelic inactivation. This model, involving inactivation of one allelic array and cis control of the active array, provides a mechanism such that individual neurons express one or a small number of receptors.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
authors.forename: A
authors.initials: A
authors.lastname: Chess
authors.forename: I
authors.initials: I
authors.lastname: Simon
authors.forename: H
authors.initials: H
authors.lastname: Cedar
authors.forename: R
authors.initials: R
authors.lastname: Axel
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004261:DNA Replication; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009115:Muridae / Q000235:genetics; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D018035:Receptors, Odorant / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012737:Sex Factors
chemical_list: D018035:Receptors, Odorant