Immunohistochemical detection of P-glycoprotein and GSTP1-1 in testis cancer.
P-glycoprotein (Pgp) and pi-class glutathione S-transferase (GSTP1-1) are thought to be correlated with multiple drug resistance. In immunohistochemical staining, non-seminomatous germ cell tumours, which are more refractory than seminomas to anti-cancer chemotherapy, frequently expressed Pgp and GSTP1-1. Western blot analysis demonstrated lower amount of GSTP1-1 in seminoma than in teratoma. These results suggest that Pgp and GSTP1-1 might contribute to drug resistance in testis cancers.
Katagiri
A
A
Department of Urology, Niigata University School of Medicine, Japan.
Tomita
Y
Y
Nishiyama
T
T
Kimura
M
M
Sato
S
S
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000328:Adult; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D004351:Drug Resistance; D005982:Glutathione Transferase / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis* / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000378:metabolism; D009367:Neoplasm Staging; D009919:Orchiectomy; D013736:Testicular Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D013737:Testis / Q000166:cytology / Q000378:metabolism / Q000473:pathology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins; D007527:Isoenzymes; D008562:Membrane Glycoproteins; D005982:Glutathione Transferase