Hormonal regulations of alkaline phosphatase, 5'-nucleotidase and gamma-glutamyltransferase activities in adult rat hepatocytes cultured in serum-free medium on collagen gel.
Primary hepatocytes were cultured on collagen gel in serum-free, alpha-modified Eagle's minimum essential medium containing 0.1 microM insulin, 0.1 microM dexamethasone, 10 mM pyruvate and supplements such as glucagon, epinephrine or growth hormone. The activities of alkaline phosphatase, 5'-nucleotidase and gamma-glutamyltransferase were assayed in cell extracts prepared from the cultures. All three enzyme activities were induced by glucagon, epinephrine or dibutyryl cAMP. The maximally effective concentration of glucagon was 5-10 nM for both alkaline phosphatase and 5'-nucleotidase and 100 nM for gamma-glutamyltransferase. Only alkaline phosphatase activity was suppressed by growth hormone, which caused marked suppression at about 1 microU (0.25 ng)/ml. Taurocholate also induced both alkaline phosphatase and gamma-glutamyltransferase activities at 1 mM.
Horiuti
Y
Y
Asahi Chemical Industry Co., Ltd. Institute for Life Science Research, Shizuoka, Japan.
Yano
K
K
Shibuya
Y
Y
D015720:5'-Nucleotidase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000032:analysis / Q000494:pharmacology; D002478:Cells, Cultured; D003094:Collagen; D016895:Culture Media, Serum-Free / Q000737:chemistry / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D005782:Gels; D005934:Glucagon / Q000494:pharmacology; D013006:Growth Hormone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000201:enzymology*; D008297:Male; D011773:Pyruvates / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013656:Taurocholic Acid / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D016895:Culture Media, Serum-Free; D005782:Gels; D007328:Insulin; D011773:Pyruvates; D013656:Taurocholic Acid; D003994:Bucladesine; D003907:Dexamethasone; D013006:Growth Hormone; D003094:Collagen; D005934:Glucagon; D013806:Theophylline; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D002118:Calcium; D004837:Epinephrine