A PCR strategy that differentiates two alleles for a single base insertion within a T repeat.
PCR amplification with modified primers, in combination with BcgI restriction fragment length polymorphism analysis, allowed us to differentiate a string of 7 Ts present in exon 7 of the 21-hydroxylase gene of man from the 8 Ts present in exon 7 of its pseudogene. This approach can substitute for hybridization with oligonucleotides, or for sequence analysis, when an allele containing a multiple repeated nucleotide sequence is to be differentiated from one with a different number of the identical nucleotide.
Lungu
O
O
Department of Microbiology, Columbia University, New York, NY 10032.
Silverstein
S
S
D000483:Alleles; D001483:Base Sequence; D001709:Biotechnology; D004247:DNA / Q000235:genetics*; D004252:DNA Mutational Analysis; D005091:Exons; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011544:Pseudogenes; D012091:Repetitive Sequences, Nucleic Acid; D013255:Steroid 21-Hydroxylase / Q000235:genetics
D004247:DNA; D013255:Steroid 21-Hydroxylase