Detection of human herpesvirus-6 in paraffin-embedded tissue of cervical cancer by polymerase chain reaction.
The polymerase chain reaction (PCR) was used to detect human herpesvirus-6 (HHV-6) DNA sequences from paraffin-embedded tissue from cervical cancer patients. Two of eight cases were positive for HHV-6 using two sets of HHV-6 primers. Hybridization of PCR products with specific radioisotope-labeled oligonucleotide probes confirmed the results. Furthermore, HHV-6 typing was possible by adapting restriction endonuclease digestion of PCR product. This method is useful for retrospective studies in investigating the etiologic role of HHV-6 in the development of human diseases.
Wang
H
H
Laboratory of Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Chen
M
M
Berneman
Z N
ZN
Delgado
G
G
DiPaolo
J A
JA
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000382:microbiology*; D015342:DNA Probes; D005260:Female; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D015342:DNA Probes