G(O), a guanine nucleotide binding protein, is expressed during neurite extension in the embryonic mouse.
The developmental pattern of expression of the G protein alpha o subunit and GAP43 were compared by immunohistochemical staining of mouse embryos. Staining for alpha o and GAP43 was identical and detected throughout the developing nervous system, and the antigens first appeared in neurons at the beginning of neuronal differentiation. GAP43 and alpha o were not detected in regions containing only neuroblasts. These observations suggest that alpha o and GAP43 may not be required for the decision to pass from neuroblast to differentiated neuron, but may play a role in signal transduction during early neuronal development.
Schmidt
C J
CJ
Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115.
Zubiaur
M
M
Valenzuela
D
D
Neer
E J
EJ
Dräger
U C
UC
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001921:Brain / Q000196:embryology*; D002454:Cell Differentiation; D005260:Female; D019922:GAP-43 Protein; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D019204:GTP-Binding Proteins / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008938:Mitosis / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D019204:GTP-Binding Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go