title: One-step enrichment of nucleated red blood cells. A potential application in perinatal diagnosis.
abstract: We describe a discontinuous triple density gradient to obtain a 25-fold enrichment of nucleated red blood cells from mononuclear cells, granulocytes, and mature red blood cells in a single centrifugation step.
authors.affiliation: Department of Gynecology and Obstetrics, Stanford University, CA 94305.
authors.forename: N M
authors.initials: NM
authors.lastname: Bhat
authors.forename: M M
authors.initials: MM
authors.lastname: Bieber
authors.forename: N N
authors.initials: NN
authors.lastname: Teng
mesh_terms: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; D018826:CD13 Antigens; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis* / Q000235:genetics; D017493:Leukocyte Common Antigens; D011247:Pregnancy / Q000097:blood; D011990:Receptors, Transferrin
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; C068126:CD71 antigen; D011990:Receptors, Transferrin; D017493:Leukocyte Common Antigens; D018826:CD13 Antigens