Monoclonal antibodies to equine arteritis virus proteins identify the GL protein as a target for virus neutralization.
Monoclonal antibodies (MAbs) to equine arteritis virus (EAV) proteins were produced and characterized. The protein specificities of eight MAbs were determined definitively by immunoprecipitation of EAV proteins expressed from vaccinia virus recombinants (VVRs). Included were two new VVRs produced for this study, expressing the M and the GL proteins, respectively. Three MAbs were determined to be N-specific and five MAbs recognized the GL protein. One GL-specific MAb, 17F5, of the IgA class, efficiently neutralized EAV infectivity. In competitive binding assays (CBAs), the N-specific MAbs defined a single antigenic domain on this protein. Four GL-specific MAbs, including MAb 17F5, demonstrated strong reciprocal competition in binding to the GL protein but differed in their virus-neutralizing ability. Thus the antigenic domain defined by these MAbs is probably composed of overlapping or closely adjacent epitopes. The fifth GL-specific MAb, a non-neutralizing antibody, may define an epitope adjacent to this antigenic domain as reciprocal CBAs demonstrated lower competition.
Deregt
D
D
Animal Diseases Research Institute, Agriculture Canada, Lethbridge, Alberta.
de Vries
A A
AA
Raamsman
M J
MJ
Elmgren
L D
LD
Rottier
P J
PJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D002460:Cell Line; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D007240:Equartevirus / Q000276:immunology*; D019204:GTP-Binding Proteins / Q000032:analysis / Q000276:immunology*; D006825:Hybridomas; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D011995:Recombination, Genetic; D014616:Vaccinia virus; D015678:Viral Structural Proteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D011994:Recombinant Proteins; D015678:Viral Structural Proteins; D019204:GTP-Binding Proteins