HS-142-1, a novel antagonist for natriuretic peptides, has no effect on the third member of membrane bound guanylate cyclases (GC-C) in T84 cells.
HS-142-1, a novel non-peptide antagonist for natriuretic peptides, exerts antagonistic actions almost equally on two similar guanylate cyclase-linked natriuretic peptide receptors (GC-A and GC-B), but has little or no effect on the binding of natriuretic peptides to a membrane protein, the so-called "clearance receptor", which binds all natriuretic peptides. The third mammalian form of membrane bound guanylate cyclases (GC-C) was identified not as a natriuretic peptide receptor, but as a receptor for heat-stable enterotoxins (STa). In this study, we examined effects of HS-142-1 on GC-C (STaR) in T84 cells and showed that HS-142-1 exerts neither agonistic nor antagonistic activity for GC-C, indicating that HS-142-1 is not a common antagonist for a family of membrane bound guanylate cyclase receptors, but a specific antagonist for the guanylate cyclase-linked natriuretic peptide receptors.
Ohyama
Y
Y
National Cardiovascular Center Research Institute, Osaka, Japan.
Miyamoto
K
K
Morishita
Y
Y
Matsuda
Y
Y
Kojima
M
M
Minamino
N
N
Kangawa
K
K
Matsuo
H
H
D009320:Atrial Natriuretic Factor / Q000502:physiology; D001427:Bacterial Toxins / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D006152:Cyclic GMP / Q000378:metabolism; D004768:Enterotoxins / Q000494:pharmacology; D029968:Escherichia coli Proteins; D005768:Gastrointestinal Hormones; D006162:Guanylate Cyclase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D045265:Natriuretic Peptides; D010455:Peptides / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D005768:Gastrointestinal Hormones; C072551:HS 142-1; D045265:Natriuretic Peptides; D010455:Peptides; D011134:Polysaccharides; C034151:heat stable toxin (E coli); C072685:guanylin; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D006152:Cyclic GMP