Evidence for the transient expression of P-450 during the neonatal period in rat.
A cytochrome P-450 N (N for Neonatal) was resolved and partially purified from 15-day-old newborn rat livers. Antibody raised in rabbit against P-450 N detects two bands in liver microsomes, the major one comigrating with the partially purified P-450 N. This protein is not expressed in lung and kidney and exhibits an unusual ontogenic profile in the liver with maximal levels in 2-week-old animals. Expression of P-450 N is not modified in animals given beta-naphthoflavone or pregnenolone 16 alpha-carbonitrile, but is markedly increased by phenobarbital. Its amino-acid composition differs from that of other P-450s and it is not recognized by antibodies directed against CYP1A, CYP2A1, CYP2B, CYP2C11, CYP3A or epoxide hydrolase. It could thus be considered as an unique P-450 expressed transiently before puberty.
Cresteil
T
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INSERM U75, Université René Descartes, Paris, France.
Marie
S
S
Sonnier
M
M
Kersual
J
J
Gonzalez
F J
FJ
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001571:Benzoflavones / Q000494:pharmacology; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000254:growth & development; D008168:Lung / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D010634:Phenobarbital / Q000494:pharmacology; D011285:Pregnenolone Carbonitrile / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D011285:Pregnenolone Carbonitrile; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital