Interleukin-1-beta, interleukin-6, and interferon-gamma in suction blister fluids of involved and uninolved skin and in sera of psoriatic patients.
Interleukin-1-beta (IL-1-beta), Interleukin-6 (IL-6) and Interferon-gamma (IFN-gamma) were measured by enzyme immunoassay (EIA) methods in blister fluids (BFs) obtained from both involved (ISBF) and non-involved skin (USBF) and in sera from 14 psoriatic patients. The same determinations were carried out in 14 sera and in 5 suction blister fluids from 14 normal subjects. IL-6 was always detectable in all skin fluids and in 3 psoriasis sera. IL-1-beta was measured only in 5 ISBFs and in 5 sera from the same patients. IFN-gamma was present in 11 ISBFs, in 5 USBFs and in 5 sera. The analysis of the levels found in the samples shows: 1) a local production of these cytokines, 2) the presence of detectable amounts of IL-6 and IFN-gamma in USBFs, and 3) a significant correlation between the IL-6 levels in the ISBFs and erythema score.
Bonifati
C
C
Third Department of Dermatology, S. Gallicano Institute, Rome, Italy.
Ameglio
F
F
Carducci
M
M
Sacerdoti
G
G
Pietravalle
M
M
Fazio
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D001768:Blister / Q000378:metabolism; D005122:Exudates and Transudates / Q000737:chemistry; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000032:analysis*; D007375:Interleukin-1 / Q000032:analysis*; D015850:Interleukin-6 / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000097:blood / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012867:Skin / Q000737:chemistry*
D007375:Interleukin-1; D015850:Interleukin-6; D007371:Interferon-gamma