title: A new extracellular protein of Pseudomonas aeruginosa PA103 regulated by regA.
abstract: The expression of exotoxin A (ExoA) from Pseudomonas aeruginosa is influenced by iron and is under the control of the regulatory gene regA. To test whether regA plays a role in the expression of other iron-regulated proteins a RegA- mutant was constructed by insertional mutagenesis. The polypeptide pattern of this mutant (PA103R) was compared with the parental strain (PA103) and a trans-complemented strain PA103R(pREX18) after growth of the strains in conditions containing low or high concentrations of iron. An iron-regulated 42 kDa protein (RRP) was identified and purified from the culture supernatant of PA103 and PA103R(pREX18) which was missing in PA103R. Database analysis of the N-terminal sequence of this regA-regulated protein (RRP) revealed no similarity to other proteins. Preliminary investigations into the function of RRP revealed that it has no proteolytic or cytotoxic activity. Using two-dimensional electrophoretic analysis of whole cells, a technique which allowed separation of over 600 polypeptides, we were unable to identify any other iron-regulated protein whose expression was regulated by regA.
authors.affiliation: Department of General and Environmental Hygiene, University of Tübingen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Wolz
authors.forename: R
authors.initials: R
authors.lastname: Lehmann
authors.forename: M L
authors.initials: ML
authors.lastname: Vasil
authors.forename: R
authors.initials: R
authors.lastname: Bischoff
authors.forename: G
authors.initials: G
authors.lastname: Döring
mesh_terms: D036002:ADP Ribose Transferases; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001427:Bacterial Toxins; D005098:Exotoxins / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000494:pharmacology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D017421:Sequence Analysis; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007501:Iron; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C072722:RegA protein, Bacteria; C089275:regA-regulated protein 42 kDa, Pseudomonas aeruginosa