title: Genomic characterization of rotavirus strains obtained from hospitalized children with diarrhoea in Bangladesh.
abstract: Faecal samples were obtained from 111 children hospitalized with acute watery gastroenteritis in a children's hospital in Bangladesh from January to December, 1989. Rotavirus was detected as the aetiologic agent in 35 (32%) of these patients. The electrophoretic pattern of ds-RNA extracted from the rotaviruses showed 11 different migration patterns (six short and five long), as well as some short and long mixed profiles. All four major G serotypes were identified by amplification of a segment of the gene for VP7 using the polymerase chain reaction. A specific serotype could be assigned in 32 (91%) cases. Serotypes G1-G4 accounted for 11%, 37%, 11%, and 23% of isolates respectively. Three (9%) mixed serotypes were identified by this method and were found to be mixtures of serotypes G1 and G4, G2 and G4, and G3 and G4.
authors.affiliation: Department of Medical Microbiology, University of Liverpool, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Tabassum
authors.forename: P
authors.initials: P
authors.lastname: Shears
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001459:Bangladesh / Q000453:epidemiology; D001483:Base Sequence; D002648:Child; D002673:Child, Hospitalized; D002675:Child, Preschool; D017931:DNA Primers; D003967:Diarrhea / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D012330:RNA, Double-Stranded / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000382:microbiology*; D012621:Seasons; D012703:Serotyping
chemical_list: D017931:DNA Primers; D012330:RNA, Double-Stranded; D012367:RNA, Viral