title: The transcript encoding the circumsporozoite antigen of Plasmodium berghei utilizes heterogeneous polyadenylation sites.
abstract: We have employed polymerase chain reaction-based techniques to examine the transcript encoding the circumsporozoite (CS) antigen, the immunodominant coat protein of the infectious stage of the murine parasite Plasmodium berghei. Earlier studies suggested that the 3' terminus of the CS message might be determined by transcription termination rather than by cleavage and polyadenylation, as in most eukaryotes. Here we report that a subset of CS messages are polyadenylated. Moreover, the poly(A) tails are added at multiple sites clustered within a short region 300 bp downstream from the stop codon. Whether 3' end heterogeneity is peculiar to the CS message or a common feature of plasmodial transcripts remains to be determined.
authors.affiliation: NYU Medical Center, Department of Medical and Molecular Parasitology, NY 10010.
authors.forename: V
authors.initials: V
authors.lastname: Ruvolo
authors.forename: R
authors.initials: R
authors.lastname: Altszuler
authors.forename: A
authors.initials: A
authors.lastname: Levitt
mesh_terms: D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics; D008969:Molecular Sequence Data; D010962:Plasmodium berghei / Q000235:genetics* / Q000254:growth & development / Q000276:immunology*; D011061:Poly A / Q000235:genetics; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; C041595:circumsporozoite protein, Protozoan; D011061:Poly A