Site of dopamine D1 receptor binding to Gs protein mapped with synthetic peptides.
We have synthesized peptides corresponding to the known sequence of the rat dopamine D1 receptor and measured the effects on binding of the Gs protein to the activated receptor. Peptides corresponding to the second and third cytoplasmic loop (amino acids 160-180 and 260-276), as well as the N-terminal part of the carboxy terminus (amino acids 375-391) were effective in preventing Gs binding to the activated receptor. Peptides representing other surface regions, e.g., the first cytoplasmic loop (87-100), the C-terminus (400-420 and 440-460) and all extracellular surface peptides (6-22, 120-137, 210-224 and 338-352) are not involved in rat dopamine D1 receptor-Gs protein interaction.
König
B
B
Ecole Polytechnique Fédérale de Lausanne Institut de Chimie Physique II CH 1015 Lausanne, Switzerland.
Grätzel
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007118:Immunoassay; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism; D051381:Rats; D017447:Receptors, Dopamine D1 / Q000302:isolation & purification / Q000378:metabolism*
D010455:Peptides; D017447:Receptors, Dopamine D1; D019204:GTP-Binding Proteins