Simple extraction of Campylobacter lipopolysaccharide and protein antigens and production of their antibodies in egg yolk.
Antigens were heat extracted from Campylobacter jejuni (LI04) and C. coli (LI020) in the presence of ethylenediaminetetraacetate (EDTA) and were recovered in the supernatant of a low-speed centrifugation. The method is simpler, safer and more efficient in extracting lipopolysaccharide (LPS) antigens than the hot phenol method. The extracted antigens (LPS plus several proteins) elicited production of antigen-specific antibodies in the egg yolk of immunized hens. Antibodies purified by polyethyleneglycol fractionation were used to detect antigens fractionated on SDS polyacrylamide gel electrophoresis.
Chandan
V
V
Department of Biology, Carleton University, Ottawa, Ontario, Canada.
Fraser
A D
AD
Brooks
B W
BW
Yamazaki
H
H
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D017000:Campylobacter coli / Q000276:immunology*; D016123:Campylobacter jejuni / Q000276:immunology*; D002498:Centrifugation; D002645:Chickens; D003429:Cross Reactions; D004492:Edetic Acid; D004530:Egg Yolk / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000276:immunology / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D004492:Edetic Acid