title: Enrichment of rab11, a small GTP-binding protein, in gastric parietal cells.
abstract: Parietal cell secretion of acid requires the coordinated fusion of H(+)-K(+)-adenosinetriphosphatase (ATPase)-containing tubulovesicles with a secretory canalicular target membrane. We have previously reported the presence of rab2 on parietal cell tubulovesicles (L. H. Tang, S. A. Stoch, I. M. Modlin, and J. R. Goldenring. Biochem. J. 285: 715-719, 1992). Since 60% of the small GTP-binding protein sequences obtained from parietal cells were > 95% homologous with human rab11 (J. R. Goldenring, K. R. Shen, H. D. Vaughan, and I.M. Modlin. J. Biol. Chem. 268: 18419-18422, 1993), we sought to study rab11 in gastric parietal cells. A complete rab11 sequence was obtained, and the deduced amino acid sequence of rabbit rab11 was identical to that for human. Rab11 mRNA was present throughout the gastrointestinal mucosa. mRNA for both rab11 and rab2 were enriched in isolated parietal cells compared with chief cells. A polyclonal antiserum against rab11 labeled a single 25-kDa band in isolated parietal cells. Immunostaining of rat fundic tissue demonstrated prominent staining of parietal cells. Rab11 staining cosegregated with alpha-H(+)-K(+)-ATPase staining in enriched preparations of rabbit parietal cell tubulovesicles. These results suggest that rab11 is enriched in parietal cells and is associated with intracellular tubulovesicles.
authors.affiliation: Department of Surgery, Yale University School of Medicine, New Haven, Connecticut.
authors.forename: J R
authors.initials: JR
authors.lastname: Goldenring
authors.forename: C J
authors.initials: CJ
authors.lastname: Soroka
authors.forename: K R
authors.initials: KR
authors.lastname: Shen
authors.forename: L H
authors.initials: LH
authors.lastname: Tang
authors.forename: W
authors.initials: W
authors.lastname: Rodriguez
authors.forename: H D
authors.initials: HD
authors.lastname: Vaughan
authors.forename: S A
authors.initials: SA
authors.lastname: Stoch
authors.forename: I M
authors.initials: IM
authors.lastname: Modlin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010295:Parietal Cells, Gastric / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D017385:Sequence Homology; D013347:Subcellular Fractions / Q000378:metabolism; D014018:Tissue Distribution; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
chemical_list: D018076:DNA, Complementary; D019204:GTP-Binding Proteins; D012333:RNA, Messenger; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins