Stimulation of phosphoenolpyruvate carboxykinase gene expression by fatty acids.
We used the 3T3-F442A adipocytes and the FAO hepatoma cells to analyze the effect of oleate on phosphoenolpyruvate carboxykinase (PEPCK) gene expression. In serum-deprived, glucose-free medium, 1 mM oleate, bound to albumin in a 6:1 ratio, specifically stimulated PEPCK mRNA. In 3T3-F442A adipocytes, the maximum 5-fold increase occurred in 4 hours then rapidly declined to reach the basal level 20 hours later. This increase was cycloheximide-independent and actinomycin D-dependent, suggesting a direct, transcriptional effect of oleate. FAO cells also responded to oleate with a transient induction of PEPCK mRNA, although the extent of stimulation was lower. Thus, the PEPCK gene provides a useful molecular tool for studying the mechanisms by which fatty acids stimulate gene expression.
Antras-Ferry
J
J
Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement, C.N.R.S., Meudon, France.
Le Bigot
G
G
Robin
P
P
Robin
D
D
Forest
C
C
D016475:3T3 Cells; D000199:Actins / Q000096:biosynthesis; D017667:Adipocytes / Q000201:enzymology*; D000818:Animals; D016895:Culture Media, Serum-Free; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D007700:Kinetics; D008114:Liver Neoplasms, Experimental; D051379:Mice; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D013997:Time Factors
D000199:Actins; D016895:Culture Media, Serum-Free; D009829:Oleic Acids; D012333:RNA, Messenger; D003609:Dactinomycin; D019301:Oleic Acid; D003513:Cycloheximide; D010729:Phosphoenolpyruvate Carboxykinase (GTP)