title: Evidence of pestivirus RNA in human virus vaccines.
abstract: We examined live virus vaccines against measles, mumps, and rubella for the presence of pestivirus RNA or of pestiviruses by reverse transcription PCR. Pestivirus RNA was detected in two measles-mumps-rubella combined vaccines and in two monovalent vaccines against mumps and rubella. Nucleotide sequence analysis of the PCR products indicated that a modified live vaccine strain used for immunization of cattle against bovine viral diarrhea is not responsible for the contamination of the vaccines.
authors.affiliation: Animal Center for Biomedical Research, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Harasawa
authors.forename: T
authors.initials: T
authors.lastname: Tomiyama
mesh_terms: D001483:Base Sequence; D001908:Diarrhea Viruses, Bovine Viral / Q000145:classification / Q000235:genetics; D004338:Drug Combinations; D004340:Drug Contamination; D008458:Measles Vaccine / Q000737:chemistry / Q000592:standards; D022542:Measles-Mumps-Rubella Vaccine; D008969:Molecular Sequence Data; D009108:Mumps Vaccine / Q000737:chemistry / Q000592:standards; D010576:Pestivirus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification*; D012411:Rubella Vaccine / Q000737:chemistry / Q000592:standards; D012680:Sensitivity and Specificity; D014613:Vaccines, Attenuated
chemical_list: D004338:Drug Combinations; D008458:Measles Vaccine; D022542:Measles-Mumps-Rubella Vaccine; D009108:Mumps Vaccine; D012367:RNA, Viral; D012411:Rubella Vaccine; D014613:Vaccines, Attenuated