The effect of maternal ethanol intoxication on erythrocyte ghost fluidity in new-born rat pups.
We administered ethanol to pregnant rats and determined cholesterol, lipid phosphorus and fluorescence anisotropy of diphenylhexatriene (DPH) and of trimethylaminophenyl hexatriene (TMA-DPH) in erythrocyte ghosts of newborn pups and of their mothers. Cholesterol content was different in dams and pups and changed after treatment. Age, but not ethanol, affected lipid phosphorus. Either age (dams versus pups) or treatment affected the curves of DPH fluorescence anisotropy (r) versus temperature (T), but those of TMA-DPH did not change, indicating that only the inner core of the membrane was influenced. We conclude that adult and erythrocyte ghosts differed for the lipid composition, for the dependence of r on T (for DPH) and for the effects of ethanol dosing on these parameters.
Arienti
G
G
Department of Biochemistry, Medical School, University of Perugia, Italy.
Battistoni
F
F
Carlini
E
E
Di Renzo
G C
GC
Cosmi
E V
EV
Reboldi
G P
GP
D000818:Animals; D000831:Animals, Newborn; D002784:Cholesterol / Q000097:blood; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000097:blood*; D005454:Fluorescence Polarization; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D013050:Spectrometry, Fluorescence
D004161:Diphenylhexatriene; D000431:Ethanol; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; D002784:Cholesterol