The chitinase encoding Tn7-based chiA gene endows Pseudomonas fluorescens with the capacity to control plant pathogens in soil.
A transposon system based on Tn7 carrying the chiA gene (encoding chitinase) of Serratia marcescens was established. The transposon was introduced by conjugation into Pseudomonas fluorescens as a stable integrate expressing and secreting the active chitinase enzyme. The resulting strain was shown to control the plant pathogen Rhizoctonia solani in soil.
Koby
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S
Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Schickler
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H
Chet
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Oppenheim
A B
AB
D002688:Chitinases / Q000235:genetics*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology*; D011551:Pseudomonas fluorescens / Q000235:genetics*; D012232:Rhizoctonia; D012706:Serratia marcescens / Q000201:enzymology / Q000235:genetics*; D012988:Soil Microbiology
D004251:DNA Transposable Elements; D002688:Chitinases