Influence of uraemia on the determination of blood glycohaemoglobin by HPLC, electrophoresis and affinity chromatography in diabetic and non-diabetic patients.
Glycohaemoglobin in human blood can be determined by several methods. We investigated three methods used in daily practice, viz. affinity chromatography, HPLC and electrophoresis. We examined the influence of uraemia in diabetic and non-diabetic patients on the results obtained with HPLC and electrophoresis and compared these with the results obtained with affinity chromatography. Our current method (electrophoresis) proved to be very sensitive to uraemia giving falsely elevated levels of HbA1. HPLC was not disturbed by uraemia.
Herruer
M H
MH
Department of Clinical Chemistry, Stichting Deventer Ziekenhuizen, Deventer, The Netherlands.
van Kooten
E A
EA
Sluiter
H E
HE
Zuijderhoudt
F M
FM
D000293:Adolescent; D000328:Adult; D000368:Aged; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014508:Urea / Q000097:blood; D014511:Uremia / Q000097:blood*
D006442:Glycated Hemoglobin; D014508:Urea