Pantropic retroviral vector-mediated gene transfer, integration, and expression in cultured newt limb cells.
Limb regeneration is a unique developmental phenomenon restricted to certain urodeles in which limb cells dedifferentiate and produce the blastema and then redifferentiate into the tissues that compose the missing part. Genetic modification of the blastema cells would greatly facilitate understanding the programmed gene expression that results in the reconstitution of the limb. To test whether pantropic retroviral vectors pseudotyped with the vesicular stomatitis virus G glycoprotein could mediate gene transfer into blastema cells, we infected a stable newt limb cell line and demonstrated integration and expression of the provirus. Thus, pantropic retroviral vectors offer a new tool for the study of limb regeneration and other developmental phenomena in amphibia.
Burns
J C
JC
Department of Pediatrics, UCSD School of Medicine, La Jolla 92093-0609.
Matsubara
T
T
Lozinski
G
G
Yee
J K
JK
Friedmann
T
T
Washabaugh
C H
CH
Tsonis
P A
PA
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D017931:DNA Primers; D005121:Extremities; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D012038:Regeneration; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology; D012455:Salamandridae; D016662:Virus Integration
D017931:DNA Primers