Age-related changes in antioxidant defence mechanisms and peroxidation in isolated hepatocytes from spontaneously hypertensive and normotensive rats.
The effects of age and hypertension on the antioxidant defence systems and the lipid peroxidation in rat isolated hepatocytes were studied. Four different age groups (1, 3, 6 and 12 months) were considered in spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. Age-associated changes were observed on vitamin E status, glutathione (GSH) level, MDA formation and glutathione peroxidase (GSH-Px) activity in both strains. Maximal levels or activities of these parameters were found at 3 and 6 months, except for MDA which was low at 3 months. Then, a fall was observed at 12-month-old compared to 6-month values. In addition, GSH-Px activity was significantly lower in SHR than in WKY rats, except at the age of one month. The decrease of this enzyme activity could induce an increased cellular generation of radical species and lipid peroxidation, which might be link to hypertension.
Véricel
E
E
INSERM U352, Chimie Biologique, INSA-Lyon, Villeurbanne, France.
Narce
M
M
Ulmann
L
L
Poisson
J P
JP
Lagarde
M
M
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D051381:Rats; D011918:Rats, Inbred SHR / Q000378:metabolism*; D011921:Rats, Inbred WKY; D013858:Thiobarbiturates / Q000032:analysis; D014810:Vitamin E / Q000032:analysis
D000975:Antioxidants; D013858:Thiobarbiturates; D014810:Vitamin E; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D005978:Glutathione; C029684:thiobarbituric acid