title: Evidence for preservation of epithelial function in cryopreserved porcine and human bronchi.
abstract: Porcine and human bronchi have been investigated in vitro without or after storage at -196 degrees C in Krebs-Henseleit solution containing 2.0 M dimethyl sulphoxide and 0.1 M sucrose as cryoprotectants. In bronchi from both species maximal post-thaw contractile responses to acetylcholine (ACh) were reduced by about 25-30% compared to unfrozen bronchi. To assess the viability of bronchi and endothelium-denuded rat aortic strips, was employed. The release of an epithelium-derived inhibitory factor (EpDIF) was induced by ACh and assessed in terms of concentration-dependent relaxation of the endothelium-denuded rat aortae. Following removal of bronchial epithelium, ACh failed to elicit any relaxation of rat aorta. With cryopreserved bronchi from both pig and human about 5 and 30 times higher concentrations of ACh, respectively, were required to elicit the same relaxant response as with unfrozen bronchi. The results suggest that after the freezing-thawing process both smooth muscle and epithelial function is largely preserved and provide support for the use of cryopreservation for storage of airway preparations for pharmacological studies.
authors.affiliation: Sandoz Pharma Ltd, Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Vacciana
authors.forename: A B
authors.initials: AB
authors.lastname: Ebeigbe
authors.forename: J
authors.initials: J
authors.lastname: Hasse
authors.forename: L
authors.initials: L
authors.lastname: Swoboda
authors.forename: E
authors.initials: E
authors.lastname: Müller-Schweinitzer
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D001685:Biological Factors / Q000378:metabolism; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D016084:Bronchoconstriction; D015925:Cryopreservation; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D009994:Osmolar Concentration; D051381:Rats; D013552:Swine; D014664:Vasodilation
chemical_list: D001685:Biological Factors; C067763:epithelium-derived inhibitory factor; D000109:Acetylcholine