Proliferation of immature T cells within the splenocytes of athymic mice by Pseudomonas exotoxin A.
Pseudomonas aeruginosa exotoxin A has been shown to stimulate splenocytes from athymic nude mice. The present studies were performed to characterize the exotoxin A-responsive cells within the splenocytes of athymic nude mice. The results of these studies indicate that exotoxin A-responsive cells were represented in the nylon wool-adherent cell population and in the Ig-depleted splenocyte population (IgNA). Flow microfluorimetry analysis indicated that exotoxin A induced the expansion of Thy1+, CD3-, CD4-, and CD8- cells which also expressed the heat-stable antigen (HSA), an antigen expressed on immature T lymphocytes. Depletion of Thy1+ cells abrogated the exotoxin A-induced proliferation; however, depletion of CD4+ and CD8+ cells did not affect the exotoxin A-induced proliferation of IgNA cells. Thus, the results indicate that exotoxin A stimulates the proliferation of immature T cells within the splenocytes of nude mice that are Thy1+, HSA+, CD3-, CD4-, CD8-.
Dixon
D M
DM
Department of Molecular Microbiology and Immunology, University of Missouri-Columbia, School of Medicine 65212.
Misfeldt
M L
ML
D036002:ADP Ribose Transferases; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D001427:Bacterial Toxins; D015704:CD4 Antigens; D016827:CD8 Antigens; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D005098:Exotoxins / Q000494:pharmacology*; D005260:Female; D016130:Immunophenotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011550:Pseudomonas aeruginosa; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D015704:CD4 Antigens; D016827:CD8 Antigens; D005098:Exotoxins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A