Estrogens determination in urine by RP-HPLC with UV detection.
A new method for the determination of estrogens by high performance liquid chromatography with UV detection at 380 nm is reported. The estrogens after extraction with Bond-Elut C18 cartridge were hydrolyzed, and derivatized with 7-chloro-4-nitro-1,3-benzoxadiazole in the presence of 18-crown-6 ether and K2CO3 and their derivatives were separated on a Hypersyl ODS 5 microns column with methanol-water (75:25 v/v) as the mobile phase. The relative standard deviation (RSD) of the method ranged from 2.1 to 3.2% and the average recovery was 98.1%.
Tirendi
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Istituto di Chimica Farmaceutica e Tossicologia, Universitá di Cantania, Italy.
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D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004967:Estrogens / Q000652:urine*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D004967:Estrogens