Apoptosis associated DNA fragmentation in epileptic brain damage.
We have examined the epileptic brain damage generated by intra-amygdaloid administration of kainic acid (KA). In the vulnerable CA3 region of the rat hippocampus, neurones developed apoptotic properties since, (i) their nuclei were positively stained with a selective in situ DNA fragmentation staining method; (ii) DNA cleavage into internucleosome-sized fragments was observed on agarose gels. Our results suggest that epileptic seizures generated in the amygdala by KA trigger apoptotic cell damage in pyramidal CA3 neurones.
Pollard
H
H
INSERM U 29, Laboratoire de Neurobiologie et Physiopathologie du Développement, Hôpital de Port Royal, Paris, France.
Cantagrel
S
S
Charriaut-Marlangue
C
C
Moreau
J
J
Ben Ari
Y
Y
D000679:Amygdala; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001927:Brain Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D006624:Hippocampus / Q000473:pathology; D007267:Injections; D007608:Kainic Acid / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D004247:DNA; D007608:Kainic Acid