Studies on Fusarium solani. Cross-reactivity among Fusarium species.
Allergenic cross-reactivity among three Fusarium species (F. solani, F. equiseti, and F. moniliforme) was determined by crossed immunoelectrophoresis (CIE), ELISA inhibition, and immunoprint-inhibition analysis. Inhibiting immunoprint was used to assess the cross-reactivity of individual allergenic proteins in culture filtrate (CF), separated on SDS-PAGE. Dose-response inhibition of band 14 kDa in immunoprints of F. solani CF by antigens of F. solani CF, F. equiseti CF, and F. moniliforme CF demonstrated significant cross-reactivity of allergenic proteins on analysis of densitometric scans. CIE and ELISA inhibition using hyperimmune sera raised in rabbits against F. solani CF antigen also demonstrated shared antigenicity among Fusarium species. Collectively, this study revealed the presence of shared as well as unique antigenic and allergenic determinants in F. solani CF, F. equiseti CF, and F. moniliforme CF antigens.
Verma
J
J
Centre for Biochemical Technology, Delhi, India.
Gangal
S V
SV
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000946:Antigens, Fungal / Q000276:immunology*; D002352:Carrier Proteins; D003429:Cross Reactions / Q000276:immunology*; D003470:Culture Media; D003720:Densitometry; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005670:Fusarium / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D015151:Immunoblotting; D007123:Immunoelectrophoresis, Two-Dimensional; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000276:immunology*; D011817:Rabbits
D000485:Allergens; D000946:Antigens, Fungal; D002352:Carrier Proteins; D003470:Culture Media; D007106:Immune Sera; D007073:Immunoglobulin E