title: Toxicological analysis of whole blood samples by means of Bond-Elut Certify columns and gas chromatography with nitrogen-phosphorus detection.
abstract: The application of Bond-Elut Certify solid-phase extraction columns to the systematic toxicological analysis of whole blood was evaluated. The reproducibility of the extraction was tested with thirteen drugs varying in physico-chemical properties. Analysis was performed with capillary gas chromatography with nitrogen-selective detection. The recoveries were reproducible, as long as other limiting factors, e.g., chromatographic behaviour or volatility, do not play a significant role. The effect of limiting chromatographic behaviour was studied in more detail with the more sensitive mass spectrometry with selected-ion monitoring after converting the extracted morphine into its ditrimethylsilyl derivative.
authors.affiliation: Forensic Science Laboratory, Rijswijk, Netherlands.
authors.forename: P G
authors.initials: PG
authors.lastname: Zweipfenning
authors.forename: A H
authors.initials: AH
authors.lastname: Wilderink
authors.forename: P
authors.initials: P
authors.lastname: Horsthuis
authors.forename: J P
authors.initials: JP
authors.lastname: Franke
authors.forename: R A
authors.initials: RA
authors.lastname: de Zeeuw
mesh_terms: D001774:Blood Chemical Analysis; D002849:Chromatography, Gas / Q000379:methods*; D064420:Drug-Related Side Effects and Adverse Reactions; D006801:Humans; D009020:Morphine / Q000097:blood; D009584:Nitrogen; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010758:Phosphorus; D015203:Reproducibility of Results
chemical_list: D004364:Pharmaceutical Preparations; D010758:Phosphorus; D009020:Morphine; D009584:Nitrogen