Selective detection of chlorine-containing compounds by gas chromatography/chemical reaction interface mass spectrometry.
Chemical reaction interface mass spectrometry (CRIMS) was studied as a gas chromatographic detection technique for chlorine-containing compounds. Both SO2 and HBr were tested as reactant gases. With SO2, a detection limit of 50 pg of diazepam and a linear range of 4 orders of magnitude were achieved, and the experimental data were reproducible. With HBr, the detection limit was 10 ng of diazepam and the linear dynamic range was only 2 orders of magnitude. The possible pharmacological application of CRIMS was studied using urine spiked with diazepam and several of its metabolites, and the results show CRIMS to be a simple but potentially powerful method in drug metabolism studies.
Song
H
H
Department of Pharmacology, George Washington University, Washington, D.C. 20037.
Abramson
F P
FP
D000143:Acids / Q000737:chemistry; D000328:Adult; D014151:Anti-Anxiety Agents / Q000032:analysis* / Q000378:metabolism / Q000652:urine; D002713:Chlorine / Q000032:analysis* / Q000737:chemistry; D003975:Diazepam / Q000032:analysis* / Q000378:metabolism / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D018054:Hydrobromic Acid; D012680:Sensitivity and Specificity; D013458:Sulfur Dioxide / Q000737:chemistry
D000143:Acids; D014151:Anti-Anxiety Agents; D013458:Sulfur Dioxide; D018054:Hydrobromic Acid; D002713:Chlorine; D003975:Diazepam