[An in vitro study on the activation of HSV-1 latent infection in the trigeminal ganglion].
Latent infection in the trigeminal ganglion (TG) was established by inoculating HSV-1 strain Mckrae on the cornea of New Zealand white rabbits. From PI day 30 to day 100, we removed the TG at intervals to establish primary cell cultures. HSV-1 antigen was positive in the TG culture cells on PI day 8 and CPE appeared on PI day 10. All latently infected TG culture cells showed spontaneous infection, while CPE and HSV-1 antigen were positive in PI 2 to 3 weeks. We thus established a cell model in which HSV-1 latent infection in TG was activated in vitro. This may serve as a new approach to study the mechanism of HSV-1 latent infection in TG.
Xie
L X
LX
Institute of Ophthalmology, Shandong Academy of Medical Sciences, Qingdao.
Dong
X G
XG
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D003389:Cranial Nerve Diseases / Q000382:microbiology; D018259:Herpesvirus 1, Human / Q000302:isolation & purification*; D007150:Immunohistochemistry; D016849:Keratitis, Herpetic / Q000382:microbiology*; D011817:Rabbits; D012668:Trigeminal Ganglion
D000956:Antigens, Viral