Distribution of hydroxylated vitamin D metabolites [25OHD3 and 1,25(OH)2D3] in domestic pigs: evidence that 1,25(OH)2D3 is stored outside the blood circulation?
1. The distribution of 25-hydroxycholecalciferol (25OHD3) and 1,25-dihydroxycholecalciferol [1,25(OH)2D3] in various organs from domestic pigs was examined by HPLC. 2. Plasma levels of both metabolites corresponded to those found in healthy human subjects. 3. Tissue concentrations of 25OHD3 in fat, kidney, liver, and intestinal mucosa were low (< 1/3 of plasma levels), whereas tissue concentrations of 1,25(OH)2D3 exceeded plasma levels by factors 3-7, adipose tissue concentrations being the highest. 4. Substantial amounts of activated vitamin D are stored outside the blood-streams and may actively participate in vitamin D and calcium homeostasis.
Rungby
J
J
University Department of Endocrinology and Metabolism, Aarhus County Hospital, Denmark.
Mortensen
L
L
Jakobsen
K
K
Brock
A
A
Mosekilde
L
L
D000818:Animals; D002112:Calcifediol / Q000378:metabolism*; D002117:Calcitriol / Q000097:blood / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D005260:Female; D006706:Homeostasis / Q000502:physiology; D013552:Swine; D014018:Tissue Distribution
D002117:Calcitriol; D002112:Calcifediol; D002118:Calcium