Induction of macrophage antitumor activity by a mycobacterial fraction. In vivo and in vitro studies.
A Methanol Extraction Residue (MER) of BCG tubercle bacillus induced cytostatic activity in vitro against the murine tumor-cell lines YAC-1 and MOPC-315 in resident murine peritoneal macrophages isolated from BALB/c mice. The induction of antitumor activity was not associated with increase in release of TNF-alpha. Macrophages from mice hyperimmunized with MER (MER 3x) or from mice injected once with MER in aqueous suspension released more PGE2 following stimulation in vitro with LPS. Macrophages cultured with either MER or MER+LPS prolonged the survival time of mice bearing palpable RPC5 plasmacytoma s.c. tumors.
Rashid
G
G
Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Israel.
Gittel
H
H
Ben-Efraim
S
S
D000818:Animals; D002478:Cells, Cultured; D007167:Immunotherapy; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D010954:Plasmacytoma / Q000628:therapy; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha