title: First-trimester human trophoblast is class II major histocompatibility complex mRNA+/antigen.
abstract: Lack of expression of the polymorphic class I and class II MHC antigens in the cytotrophoblast is one of the major factors determining the privileged immunologic status of the placenta. In this report, we show that first-trimester human placental cytotrophoblast cells display moderate to strong expression of class II MHC (HLA-DR alpha and -DR beta) and Ii chain transcripts, apparently in absence of detectable class II antigens and Ii chain. In addition, DR alpha, DR beta, and Ii mRNAs, but not antigens, are consistently upregulated by IFN-gamma. Constitutive expression and upregulation of mRNAs are detectable in trophoblast cells kept in short term as well as prolonged (2-3 weeks) culture. These results are reminiscent of an analogous mRNA+/antigen- dissociation occurring, in the case of class I MHC gene products, in a subpopulation of first-trimester cytotrophoblast cells. Thus, analogous mechanisms prevent the expression of potentially hazardous class I and II allodeterminants at early stages of semiallogeneic pregnancy.
authors.affiliation: Immunology Laboratory, Regina Elena Institute, Rome, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Giacomini
authors.forename: S
authors.initials: S
authors.lastname: Tosi
authors.forename: C
authors.initials: C
authors.lastname: Murgia
authors.forename: F
authors.initials: F
authors.lastname: Nobili
authors.forename: S
authors.initials: S
authors.lastname: Gaetani
authors.forename: R
authors.initials: R
authors.lastname: Gambari
authors.forename: M R
authors.initials: MR
authors.lastname: Nicotra
authors.forename: G
authors.initials: G
authors.lastname: Simoni
authors.forename: F
authors.initials: F
authors.lastname: Maggi
authors.forename: P G
authors.initials: PG
authors.lastname: Natali
mesh_terms: D015152:Blotting, Northern; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D017403:In Situ Hybridization; D007371:Interferon-gamma / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000276:immunology; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis*; D014327:Trophoblasts / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D006684:HLA-DR Antigens; D012333:RNA, Messenger; D007371:Interferon-gamma