Hematological, blood biochemical, and cytological bronchoalveolar lavage studies in prepubertal gilts after endotoxin inhalation and ingestion.
The effect of oral intake of endotoxin (ET) combined with inhalation of ET-contaminated feed-dust was studied in 6 prepubertal gilts. The animals were given 100 mg of ET each in their ordinary feed ration and meanwhile stayed for 4 h in a continuous flow exposure chamber where the endotoxin concentration was approximately 9 micrograms/m3. Blood samples were collected during the exposure and for 8 h afterwards. Bronchoalveolar lavage (BAL) was performed 24-26 h after the exposure. Six additional animals received ordinary feed and were exposed to feed-dust without added ET. Blood samples were analysed for total white blood cell count (B-WBC) and differential count: polymorphonuclear (PMN) and mononuclear (MN); total red blood cell count (B-RBC); platelets (B-PLT); total serum bile acids (S-BA); glutamate dehydrogenase (S-GLDH); and a blood plasma prostaglandin F2 alpha metabolite (15-ketodihydro-PGF2 alpha; P-PG). The major findings were decreased numbers of MN, PMN and B-RBC 3 to 6 h after the start of the ET exposure and increased numbers of neutrophilic granulocytes in the BAL fluid of the ET-exposed animals. It is concluded that a combined oral and airborne ET challenge induces hematological and cytological changes that may contribute to the development of respiratory disorders.
Holst
H
H
Department of Clinical Chemistry, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
Edqvist
L E
LE
Kindahl
H
H
Rylander
R
R
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001772:Blood Cell Count / Q000662:veterinary; D001773:Blood Cells / Q000187:drug effects*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000139:chemically induced* / Q000473:pathology
D000393:Air Pollutants; D004731:Endotoxins