title: Matrix metalloproteinases (MMP-2 and MMP-9) of the oral cavity: cellular origin and relationship to periodontal status.
abstract: Proteolytic enzymes released by the host cells are associated with the tissue destruction in periodontal diseases. Matrix metalloproteinases (MMPs) have the primary role in this process, since, in concert, they can degrade most of the extracellular matrix components. In the present study, we investigated MMP-2 and MMP-9 in oral fluids of healthy subjects and periodontitis patients and the contributions of different oral cells to the enzyme production. The enzymograms revealed that the main gelatinase in oral rinses, crevicular fluid, and whole saliva migrated at 92 kDa. Activity was also detected at 200 kDa and 130 kDa and minor activity at 86 kDa, 72 kDa, and 40 kDa. Traces of gelatinolytic activity were also detected in pure parotid secretions. The 92-kDa enzyme was identified to MMP-9 and the 200-kDa gelatinase to MMP-2, by means of specific anti-72-kDa antiserum. Gingival keratinocytes produced mainly MMP-9, while gingival and granulation tissue fibroblasts expressed MMP-2. Glandular tissue contained mainly MMP-9, and mRNA for MMP-9 was also found in acinar epithelial cells. Periodontitis patients had significantly higher levels of MMP-9 than healthy subjects. Also, MMP-2 was elevated in periodontitis patients. Periodontal treatment reduced the amount of gelatinases dramatically. This study shows that gelatinases are produced by various cells in the oral cavity. The amount of gelatinases is elevated during periodontal disease, while conventional periodontal treatment efficiently reduces the levels these enzymes. We suggest that MMP-2 and MMP-9 could participate in tissue destruction in periodontitis.
authors.affiliation: Department of Oral Surgery, University of Oulu, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Mäkelä
authors.forename: T
authors.initials: T
authors.lastname: Salo
authors.forename: V J
authors.initials: VJ
authors.lastname: Uitto
authors.forename: H
authors.initials: H
authors.lastname: Larjava
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D017364:Collagenases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D018093:Gelatinases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D005881:Gingiva / Q000201:enzymology; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D015151:Immunoblotting; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000201:enzymology; D008297:Male; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008875:Middle Aged; D008970:Molecular Weight; D009504:Neutrophils / Q000201:enzymology; D010196:Pancreatic Elastase / Q000032:analysis / Q000378:metabolism; D010306:Parotid Gland / Q000201:enzymology; D010518:Periodontitis / Q000201:enzymology*; D015347:RNA Probes; D012463:Saliva / Q000201:enzymology*
chemical_list: D015347:RNA Probes; D010196:Pancreatic Elastase; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9