DNA-minisatellite mutations: recent investigations concerning distribution and impact on parentage testing.
At least 815 meioses were studied in the HinfI polymorphisms of DNA minisatellite loci D1S7, D2S44, D7S21, D7S22, and D12S11 in order to collect data on respective mutation rates. At locus D7S21 (probe MS31) a striking difference between the paternal and maternal mutation rate was observed (1.5% versus 0.2%). This study also describes, how to deal biostatistically with paternal mutations in parentage testing. Possible implications of mutations are illustrated by the description of 2 cases. Case 1 reports an "exclusion" of mother and father with probe MS1. Case 2 describes 2 paternal "exclusions" with probes MS31 and G3. The statistical likelihood for a paternal "exclusion" with 2 of the 5 probes is 0.13%. By omitting probe MS1, this frequency can be reduced to 0.02%. Nevertheless, the second case clearly shows, that informative blood group markers cannot be replaced by DNA polymorphisms.
Henke
J
J
Institut für Blutgruppenforschůng, Köln, Germany.
Fimmers
R
R
Baur
M P
MP
Henke
L
L
D000483:Alleles; D002874:Chromosome Mapping; D004252:DNA Mutational Analysis; D015342:DNA Probes; D004276:DNA, Satellite / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D010334:Paternity; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D004276:DNA, Satellite