Development of DNA probes for fingerprinting Aspergillus fumigatus.
Several different DNA fragments containing nonribosomal repetitive sequences have been isolated from the genome of Aspergillus fumigatus and tested as potential DNA fingerprinting probes. Eight of these clones generate 19 or more bands when hybridized to EcoRI-digested DNA of a reference strain in Southern blots, and they fall into four families. Individual clones from two families were tested and were found to generate complex Southern blot hybridization patterns which are stable within a single strain over many generations, which vary among unrelated strains, and which are amenable to computer-assisted analyses involving large numbers of strains in epidemiological studies. Clones from three of the families clustered a majority of test strains in a similar fashion in individual dendrograms based on similarity coefficients computed from band positions in Southern blot hybridization patterns. These clones therefore fulfill the major requisites for effective DNA fingerprinting probes.
Girardin
H
H
Laboratoire du Génie de l'Hygiène et des Procedés Alimentaires, Institut National de Recherche Agronomique, Massy, France.
Latgé
J P
JP
Srikantha
T
T
Morrow
B
B
Soll
D R
DR
D001228:Aspergillosis / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D016172:DNA Fingerprinting / Q000379:methods*; D015342:DNA Probes / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D015336:Molecular Probe Techniques; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D015342:DNA Probes; D004271:DNA, Fungal