Autoradiography of L-[3H]aspartate binding sites.
The distribution, pharmacology and binding properties of L-[3H]aspartate were determined in sections from rat brain. No binding was detected in the absence of sodium ions. With the addition of sodium ions to the incubation medium, binding was found to be NMDA, AMPA and CNQX insensitive, but was potently inhibited by threo-beta-hydroxyaspartate, D-aspartate and L-2,4 trans-pyrrolidine dicarboxylate; compounds which have been shown to be specific inhibitors of the sodium-dependent EAA transporter. Autoradiography of L-[3H]aspartate closely resembled the pattern of sodium-dependent D-[3H]aspartate binding. Cerebellar binding showed higher affinity and maximal levels of binding than forebrain, consistent with reports of heterogeneous populations of sodium-dependent EAA binding sites. These results suggest that under these conditions, L-[3H]aspartate specifically labels the sodium-dependent EAA transporter.
Anderson
K J
KJ
Department of Physiological Sciences, University of Florida, Gainesville 32610.
Sandler
D L
DL
D026905:Amino Acid Transport Systems; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism* / Q000494:pharmacology; D001345:Autoradiography; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002021:Buffers; D002352:Carrier Proteins / Q000378:metabolism; D003544:Cysteic Acid / Q000494:pharmacology; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D007700:Kinetics; D008297:Male; D018377:Neurotransmitter Agents; D051381:Rats; D017207:Rats, Sprague-Dawley; D017459:Receptors, Amino Acid / Q000378:metabolism; D012964:Sodium / Q000494:pharmacology; D013237:Stereoisomerism; D014316:Tritium
D026905:Amino Acid Transport Systems; D002021:Buffers; D002352:Carrier Proteins; D005971:Glutamates; D018377:Neurotransmitter Agents; D017459:Receptors, Amino Acid; D014316:Tritium; D001224:Aspartic Acid; D018698:Glutamic Acid; D012964:Sodium; D003544:Cysteic Acid; D003545:Cysteine; C013461:cysteine sulfinic acid