Differential modulation of chemical and electrical components of mixed synapses in the lobster stomatogastric ganglion.
1. Two pairs of neurons in the pyloric network of the spiny lobster, Panulirus interruptus, communicate through mixed graded chemical and rectifying electrical synapses. The anterior burster (AB) chemically inhibits and is electrically coupled to the ventricular dilator (VD); the lateral pyloric (LP) and pyloric (PY) neurons show reciprocal chemical inhibition and electrical coupling. We examined the effects of dopamine (DA), serotonin (5HT) and octopamine (Oct) on these mixed synapses to determine the plasticity possible with opposing modes of synaptic interaction. 2. Dopamine increased net inhibition at all three pyloric mixed synapses by both reducing electrical coupling and increasing chemical inhibition. This reversed the sign of the net synaptic interaction when electrotonic coupling dominated some mixed synapses, and activated silent chemical components of other mixed synapses. 3. Serotonin weakly enhanced LP-->PY net inhibition, by reducing electrical coupling without altering chemical inhibition. Serotonin reduced AB-->VD electrical coupling, but variability in its effect on the chemical component made the net effect non-significant. 4. Octopamine enhanced LP-->PY and PY-->LP net inhibition by enhancing the chemical inhibitory component without altering electrical coupling. 5. Differential modulation of chemical and electrical components of mixed synapses markedly changes the net synaptic interactions. This contributes to the flexible outputs that modulators evoke from anatomically defined neural networks.
Johnson
B R
BR
Section of Neurobiology and Behavior, S.G., Cornell University, Ithaca, NY 14853.
Peck
J H
JH
Harris-Warrick
R M
RM
D000818:Animals; D001679:Biogenic Amines / Q000494:pharmacology*; D004298:Dopamine / Q000494:pharmacology; D004594:Electrophysiology; D017952:Ganglia, Invertebrate / Q000502:physiology*; D008121:Nephropidae / Q000502:physiology*; D009433:Neural Inhibition / Q000187:drug effects; D009473:Neuronal Plasticity; D009655:Octopamine / Q000494:pharmacology; D011708:Pylorus / Q000294:innervation*; D012701:Serotonin / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects* / Q000502:physiology*
D001679:Biogenic Amines; D009655:Octopamine; D012701:Serotonin; D004298:Dopamine