Measurement of interleukin-1 liberation in zymosan air-pouch exudate in mice.
The aim of the present study was to examine whether interleukin-1 (IL-1) production is involved in the pathology of inflammation induced by zymosan in the air-pouch of mice. For this reason the IL-1 alpha level was determined in the air-pouch exudate by specific ELISA kit 4, 24, 48 h and 4-8 days after zymosan injection into preformed subcutaneous air-pouches in mice. Concurrently, some conventional parameters such as volume of exudate, its protein content and the total leukocyte count were also measured. The IL-1 alpha level reached its maximum 24 h after zymosan administration and remained elevated throughout the 8-day observation period. Exudation, accumulation of leukocytes and protein also were maximal on day 8. The effects of some anti-inflammatory agents have also been examined. Orally administered dexamethasone induced a dose-dependent reduction in IL-1 alpha, whereas indomethacin and IX 207-887, an IL-1-release inhibitor, failed to reduce the IL-1 alpha content in this model.
Erdö
F
F
Division of Pharmacology, Institute for Drug Research, Budapest, Hungary.
Török
K
K
Székely
J I
JI
D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005122:Exudates and Transudates / Q000276:immunology*; D007213:Indomethacin / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology*; D007279:Injections, Subcutaneous; D007375:Interleukin-1 / Q000378:metabolism*; D007958:Leukocyte Count / Q000187:drug effects; D051379:Mice; D011506:Proteins / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology*
D007375:Interleukin-1; D011506:Proteins; D003907:Dexamethasone; D015054:Zymosan; D007213:Indomethacin