Enhanced binding to antigen of monoclonal antibodies modified with a crosslinker.
Modification of monoclonal antibodies (mAbs) with a photo-crosslinker, sulfosuccinimidyl 2-(m-azido-o-nitrobenzamido)-ethyl-1, 3'-dithiopropionate (SAND), enhanced their antigen binding activity. By SAND modification, SV2-61 gamma mAb against human c-erbB-2 protooncogene product could immunoprecipitate approximately an 8-fold greater amount of antigen, and AB-3 mAb against bovine serum albumin (BSA) bound to BSA more efficiently than unmodified mAb as determined by an enzyme-linked immunosorbent assay. Relative binding affinity of SAND-modified AB-3 was about 4-fold of unmodified AB-3. Augmented binding of mAbs was observed with or without photosensitization.
Ueno
H
H
Department of Hygienic Chemistry, Tohoku University, Sendai, Japan.
Masuko
T
T
Wang
J
J
Hashimoto
Y
Y
D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism*; D000915:Antibody Affinity; D000941:Antigens / Q000378:metabolism*; D001386:Azides / Q000737:chemistry*; D001666:Binding Sites, Antibody; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011233:Precipitin Tests; D011518:Proto-Oncogene Proteins / Q000276:immunology; D018719:Erb-b2 Receptor Tyrosine Kinases; D013388:Succinimides / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000941:Antigens; D001386:Azides; D003432:Cross-Linking Reagents; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D013388:Succinimides; C062493:sulfosuccinimidyl-2-(3-azido-2-nitrobenzamido)ethyl-1,3'-dithiopropionate