Physeal distraction and cell proliferation in the growth plate.
We studied the cellular response to physeal distraction in the growth plates of skeletally immature rabbits. We used a new method of labelling and detection of proliferating cells with bromodeoxyuridine (BUdR) and an anti-BUdR antibody. The application of an external fixator but no distraction force produced no changes in the growth plates. After five days of distraction at a maximum force of 20 N, the growth plate became thicker, mainly because of an increase in the number of hypertrophic chondrocytes, but there was no evidence of increased cell proliferation. Recent fractures were seen at the junction of growth plate and metaphysis but the increase in bone length was insignificant. After ten days of distraction at the same maximum force, the chondrocyte columns had become disorganised and cell proliferation was significantly decreased. There was an increase in bone length due to distraction of the fracture gap. In this model, physeal distraction did not stimulate cell proliferation, but actually inhibited it. The apparent increase in growth-plate thickness produced by distraction is not due to increased cell production, but results from inhibition of endochondral ossification and the consequent accumulation of hypertrophic chondrocytes. Any growth after distraction depends on the ability of growth-plate chondrocytes to divide. The decrease in proliferative activity which we found after ten days of distraction suggests the need for caution in the use of such procedures in young children.
Apte
S S
SS
Nuffield Orthopaedic Centre, Oxford, England.
Kenwright
J
J
D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine; D002455:Cell Division; D016267:External Fixators; D006132:Growth Plate / Q000000981:diagnostic imaging / Q000473:pathology*; D015576:Hyperostosis / Q000209:etiology / Q000473:pathology*; D008297:Male; D008954:Models, Biological; D011817:Rabbits; D011859:Radiography; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery
D001973:Bromodeoxyuridine