Effect of vanadyl sulfate feeding on susceptibility to peroxidative change in diabetic rats.
The effects of vanadyl sulfate treatment on susceptibility to oxidative stress were investigated in streptozotocin-diabetic Wistar rats. A 2 x 2 factorial design was employed, with four groups of animals: 1) untreated, non-diabetic; 2) vanadyl-treated, non-diabetic; 3) untreated, diabetic; and 4) vanadyl-treated, diabetic. Vanadyl sulfate was administered as a 1.00 to 1.25 mg/ml solution in drinking water. Cataract development was entirely suppressed in vanadyl-treated compared to untreated, diabetic rats. STZ-induction of diabetes diminished glutathione (GSH) levels in liver homogenates; whereas vanadyl treatment resulted in restored levels of this nonenzymatic antioxidant. Thiobarbituric acid reactive substances (TBARS), both basal and iron-stimulated, were significantly elevated in all vanadyl-treated animals. Vanadyl treatment lowered liver glutamine synthetase activities in diabetic rats, but not in non-diabetic animals. Thus, vanadyl treatment was antioxidant in terms of cataract formation and reduced glutathione concentration in liver homogenates, pro-oxidant by reason of iron-stimulated TBARS formation and inconclusive with respect to glutamine synthetase activity. These results highlight the importance of using multiple indicators of peroxidative change in evaluating new pro-oxidant/antioxidant treatment regimens.
Thompson
K H
KH
Division of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada.
McNeill
J H
JH
D000284:Administration, Oral; D000818:Animals; D002386:Cataract / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014639:Vanadium / Q000378:metabolism / Q000494:pharmacology*; D017968:Vanadium Compounds
D017392:Thiobarbituric Acid Reactive Substances; D017968:Vanadium Compounds; D014639:Vanadium; D013311:Streptozocin; C034028:vanadyl sulfate; D005974:Glutamate-Ammonia Ligase; D005978:Glutathione