title: Experimental infection of newborn mice with murine EDIM rotavirus.
abstract: All four-day old Swiss mice whose dams were seronegative to rotavirus developed diarrhea 72 h after oral infection with EDIM rotavirus (HP strain). Electron microscopy of infected small intestine showed rotavirus particles of different sizes and morphology in the intestinal absorptive cells. In these cells rotavirus particles of 30 to 60 nm representing the various steps of virus maturation were visualized in viroplasm near the rough endoplasmic reticulum (RER). Mature rotavirus particles of 70 nm approximately were observed inside the RER of the intestinal absorptive cells. In the lamina propria rotavirus particles of approximately 30 nm and tubules were observed in phagosome-like structures of subepithelial phagocytes and were probably destroyed by lysosomal enzymes. The absence of signs of viral replication in phagocytes of lamina propria suggests that this may be the mechanism for viral antigen presentation to the host immunological system and may be one of the reasons for the fact that rotavirus infection is limited to the intestine.
authors.affiliation: Departamento de Virologia, Universidade Federal do Rio de Janeiro, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Guimarães
authors.forename: V
authors.initials: V
authors.lastname: Chagas
authors.forename: C
authors.initials: C
authors.lastname: Nozawa
authors.forename: C
authors.initials: C
authors.lastname: Takiya
authors.forename: P C
authors.initials: PC
authors.lastname: Maia
authors.forename: S O
authors.initials: SO
authors.lastname: Souza
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003967:Diarrhea / Q000382:microbiology*; D007421:Intestine, Small / Q000382:microbiology; D051379:Mice; D008854:Microscopy, Electron; D010586:Phagocytes / Q000382:microbiology; D012401:Rotavirus; D012400:Rotavirus Infections; D013997:Time Factors