Transamination and transsulphuration of L-cysteine in Ehrlich ascites tumor cells and mouse liver. The nonenzymatic reaction of L-cysteine with pyruvate.
1. The activity of cysteine aminotransferase (CAT), 3-mercaptopyruvate sulfurtransferase (MPST) and rhodanese is much lower in Ehrlich ascites tumor cells (EATC) than in mouse liver. 2. Contrary to mouse liver homogenate, no synthesis of sulphane sulphur-containing compounds from L-cysteine is observed in EATC homogenate. 3. 2-Methyl-thiazolidine-2,4-dicarboxylic acid (CP), 2-methyl-thiazolidine-4-carboxylic acid (CA) and thiazolidine-4-carboxylic acid (CF) can be used as sources of low molecular-weight thiol compounds both in EATC and mouse liver homogenate. 4. Pyruvate formed from phosphoenolpyruvate (PEP) in EATC homogenates reacts with L-cysteine (L-CYS) to CP.
Włodek
L
L
Institute of Medical Biochemistry, Medical Academy, Kraków, Poland.
Wróbel
M
M
Czubak
J
J
D000588:Amines / Q000378:metabolism; D000818:Animals; D001711:Biotransformation; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology / Q000378:metabolism*; D003542:Cystathionine gamma-Lyase / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D013455:Sulfur / Q000378:metabolism; D013466:Sulfurtransferases / Q000378:metabolism; D013861:Thiocyanates / Q000378:metabolism; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism
D000588:Amines; D005971:Glutamates; D013861:Thiocyanates; D018698:Glutamic Acid; D013455:Sulfur; D000637:Transaminases; C019968:cysteine aminotransferase; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase; C021550:3-mercaptopyruvate sulphurtransferase; D003542:Cystathionine gamma-Lyase; D003545:Cysteine; C031760:thiocyanate