Interleukin-4 acts as a potent stimulator for expression of monocyte chemoattractant JE/MCP-1 in mouse peritoneal macrophages.
The recruitment of monocyte/macrophages to inflammatory sites is one of the important events in inflammatory reactions. We show herein that interleukin-4 (IL-4) acts as a potent stimulator for expression of monocyte chemoattractant JE/MCP-1 in mouse peritoneal macrophages. IL-4 induced the JE/MCP-1 gene expression in dose and time dependent fashion. Run-on assay suggests that IL-4 stimulates the JE/MCP-1 gene expression at transcriptional level. Monocyte chemotactic activity was detected in culture medium of the cytokine-treated cells. The chemotactic activity in the culture supernatant was completely neutralized by anti-JE/MCP-1 antiserum.
Kikuchi
H
H
Department of Oral Microbiology, Meikai University, School of Dentistry, Saitama, Japan.
Hanazawa
S
S
Takeshita
A
A
Nakada
Y
Y
Yamashita
Y
Y
Kitano
S
S
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000096:biosynthesis* / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D003470:Culture Media; D016207:Cytokines / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D015847:Interleukin-4 / Q000494:pharmacology*; D007700:Kinetics; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D014158:Transcription, Genetic / Q000187:drug effects
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D003470:Culture Media; D016207:Cytokines; D015847:Interleukin-4