title: Accuracy of plasma glucose measurements in the hypoglycemic range.
abstract: OBJECTIVE
  This study was designed to evaluate three different enzymatic methods for glucose measurement in plasma samples with special emphasis on glucose concentrations in the hypoglycemic range.
  
  
  METHODS
  Glucose dehydrogenase (Hemo-Cue analyzer), glucose oxidase (YSI analyzer), and hexokinase (Abbott analyzer) methods were used to measure plasma samples that were obtained during research studies.
  
  
  RESULTS
  Mean glucose concentrations (n = 240) were 5.3 +/- 0.2, 5.4 +/- 0.2, and 5.6 +/- 0.2 mM (95.6 +/- 3.9, 96.7 +/- 3.9, and 101.6 +/- 4.0 mg/dl) using glucose dehydrogenase, glucose oxidase, and hexokinase, respectively (NS). In the hypoglycemic range, mean glucose concentrations with each method retained the same hierarchy of measurements: 2.7 +/- 0.05, 2.8 +/- 0.04, and 2.9 +/- 0.03 mM (48.4 +/- 0.9, 50.6 +/- 0.8, and 52.3 +/- 0.6 mg/dl) by glucose dehydrogenase, glucose oxidase, and hexokinase, respectively (P < 0.005). Individual glucose dehydrogenase measurements (n = 240) correlated well with glucose oxidase and hexokinase, r = 0.99, and were considerably easier to perform at the bedside. The differences between the glucose measurement methods were consistent and similar in low, normal, and high concentration ranges.
  
  
  CONCLUSIONS
  We conclude that any interpretation or comparison of critical clinical and research measurements of glucose in different settings take into account methodological differences, particularly in the hypoglycemic range.
authors.affiliation: Department of Medicine, Harbor-UCLA Medical Center, Torrance 90502.
authors.forename: P M
authors.initials: PM
authors.lastname: Genter
authors.forename: E
authors.initials: E
authors.lastname: Ipp
mesh_terms: D000704:Analysis of Variance; D001786:Blood Glucose / Q000032:analysis*; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases; D005949:Glucose Oxidase; D006593:Hexokinase; D006801:Humans; D007003:Hypoglycemia / Q000097:blood* / Q000175:diagnosis; D012016:Reference Values; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods
chemical_list: D001786:Blood Glucose; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D005949:Glucose Oxidase; D006593:Hexokinase