Genetic interrelationships of proteolytic Clostridium botulinum types A, B, and F and other members of the Clostridium botulinum complex as revealed by small-subunit rRNA gene sequences.
The phylogenetic interrelationships of members of the Clostridium botulinum complex of species was investigated by direct sequencing of their 16S rRNA genes. Comparative analysis of the 16S rRNA sequences demonstrated the presence of four phylogenetically distinct lineages corresponding to: i) proteolytic C. botulinum types A, B, and F, and C. sporogenes, ii) saccharolytic types B, E and F, iii) types C and D and C. novyi type A, and iv) type G and C. subterminale. The phylogenetic groupings obtained from the 16S rRNA were in complete agreement with the four divisions recognised within the the 'species complex' on the basis of phenotypic criteria.
Hutson
R A
RA
Department of Microbiology, AFRC Institute of Food Research, Reading Laboratory, UK.
Thompson
D E
DE
Lawson
P A
PA
Schocken-Itturino
R P
RP
Böttger
E C
EC
Collins
M D
MD
D001483:Base Sequence; D003014:Clostridium botulinum / Q000145:classification / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S