title: c-fos and jun gene expression in murine precursor B lymphocytes developed in the interleukin-7-dependent bone marrow cell culture.
abstract: We analyzed the expression of c-fos and jun family gene in murine pre-B cells developed in the interleukin-7-(IL-7) dependent bone marrow cell culture. The c-fos gene was expressed in the pre-B cells. The c-fos RNA became undetectable after IgM+ B cells were developed in the culture. The c-fos expression in the pre-B cells was IL-7-dependent. However, the c-fos RNA was not induced when the pre-B cells cultured without IL-7 for 6 h were restimulated with IL-7. The expression of c-jun RNA was slightly induced in the restimulated pre-B cells. The junB and junD RNA were the steady state level in the cells. These gene products formed AP-1 molecule in the pre-B cells. These results suggest that the AP-1 plays a role in the IL-7-dependent pre-B cell development.
authors.affiliation: Department of Immunology, ICMR, Kobe University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: S
authors.initials: S
authors.lastname: Imoto
authors.forename: J
authors.initials: J
authors.lastname: Phuchareon
authors.forename: T
authors.initials: T
authors.lastname: Tokuhisa
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001483:Base Sequence; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D015870:Gene Expression; D016762:Genes, fos / Q000502:physiology*; D016758:Genes, jun / Q000502:physiology*; D015851:Interleukin-7 / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis
chemical_list: D015851:Interleukin-7; D016755:Proto-Oncogene Proteins c-jun