Study on the activities of testes and accessory sex glands after losulazine treatment in rats.
Losulazine hydrochloride, an antihypertensive agent, was administered intraperitoneally to adult male Wistar rats in doses of 1, 2 and 5 mg/kg/day for 1 seminiferous epithelium cycle to determine its effects on testicular steroidogenesis, spermatogenesis and accessory sex gland function. Administration of low doses of losulazine (1 and 2 mg/kg) resulted in a significant elevation of the steriodogenic key enzymes activity along with significant depletion of cholesterol content in testicular tissue. In addition, a significant rise in acid phosphatase activity in testes and prostate, and fructose content in accessory sex glands was also observed after low doses of losulazine. However, treatment with a higher dose of losulazine (5 mg/kg) significantly reduced the activities of testicular steroidogenic enzymes and acid phosphatase, and the content of fructose in accessory sex glands. Quantitative evaluation of the different varieties of germ cells at stage VII of the seminiferous epithelium cycle and epididymal sperm count in all losulazine-treated rats revealed a persistant prominent detrimental change in spermatogenesis. The results of our present experiment demonstrate an adverse action of losulazine treatment on functional activities of testes and accessory sex glands in adult rats.
Ray
A
A
Department of Physiology, University College of Science, Calcutta, India.
Chatterjee
S
S
Biswas
N M
NM
D015067:17-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors; D015096:3-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors; D000704:Analysis of Variance; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005837:Genitalia, Male / Q000187:drug effects*; D006913:Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors*; D007274:Injections, Intraperitoneal; D008297:Male; D010879:Piperazines / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000201:enzymology
D000959:Antihypertensive Agents; D010879:Piperazines; C036654:losulazine hydrochloride; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; D006913:Hydroxysteroid Dehydrogenases