Differential expression of several E2-type ubiquitin carrier protein genes at different developmental stages in Arabidopsis thaliana and Nicotiana sylvestris.
We characterized three genes encoding different E2-type ubiquitin carrier proteins involved in the ubiquitin-mediated proteolytic pathway: UbcAt3 shows homologies to the yeast CDC34 gene and Ub-cAt4a and UbcAt4b are two different genes homologous to the Ubc1/4/5 subfamily in yeast. Their accumulation was analysed and compared with that of the different families encoding polyubiquitins, as well as the monoubiquitin fusion protein, which is considered as a marker for cell division, during various developmental stages including G0/S transition and senescence of higher plant cells. Our results imply that these Ubc genes are under the control of complex mechanisms, and are differentially regulated, but not necessarily co-regulated with ubiquitin genes. Even the closely related UbcAt4a and UbcAt4b genes of the same multigene subfamily are controlled by distinct regulatory mechanisms.
Genschik
P
P
Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
Durr
A
A
Fleck
J
J
D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D064173:Anaphase-Promoting Complex-Cyclosome; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics; D002453:Cell Cycle / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D008025:Ligases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010947:Plants, Toxic; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D044763:Ubiquitin-Conjugating Enzymes; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases; D014452:Ubiquitins / Q000378:metabolism
D002352:Carrier Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014452:Ubiquitins; C577449:CDC34 protein, S cerevisiae; C577447:CDC34 protein, human; C077072:UBC7 protein, S cerevisiae; C079437:UBE2L3 protein, human; D044763:Ubiquitin-Conjugating Enzymes; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D008025:Ligases