Acrylamide induced immunosuppression in rats and its modulation by 6-MFA, an interferon inducer.
In the present communication, we describe acrylamide (ACR) induced immunotoxicity and its modulation by an interferon inducer, the 6th mycelial fraction acetone (6-MFA) of Aspergillus ochraceus ATCC 28706. ACR administration to rats produced a significant decrease in the weight of spleen (p < 0.001), thymus (p < 0.001) and mesenteric lymph nodes (p < 0.05). A decrease in cellularity of spleen (p < 0.001), thymus (p < 0.001), bone marrow (p < 0.001) and circulating blood lymphocyte population (p < 0.001) was also recorded. ACR suppressed the humoral as well as cell mediated immunity as assessed by erythrocyte antibody complement (EAC)-rosettes (p < 0.001), hemagglutination titre (p < 0.001), PFC (p < 0.001) and the delayed type hypersensitivity response against sheep red blood cells (SRBC, p < 0.001). ACR treated immunosuppressed rats when treated with 6-MFA restored the circulating lymphocyte number to the normal level and a partial recovery in the weight of spleen and thymus. Potentiation of EAC-rosettes, hemagglutination titre, IgM-PFC and DTH response against SRBC was observed. It is concluded that 6-MFA ameliorate the ACR induced toxicity. This study may be of significance in prevention of ACR toxicity.
Zaidi
S I
SI
Preventive Toxicology Division, Industrial Toxicology Research Centre, Lucknow, India.
Raisuddin
S
S
Singh
K P
KP
Jafri
A
A
Husain
R
R
Husain
M M
MM
Mall
S A
SA
Seth
P K
PK
Ray
P K
PK
D020106:Acrylamide; D000178:Acrylamides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000818:Animals; D004912:Erythrocytes / Q000276:immunology; D005656:Fungal Proteins / Q000494:pharmacology*; D006384:Hemagglutination; D006462:Hemolytic Plaque Technique; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007109:Immunity / Q000187:drug effects*; D007369:Interferon Inducers / Q000494:pharmacology*; D007958:Leukocyte Count / Q000187:drug effects; D008221:Lymphoid Tissue / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D012397:Rosette Formation; D012756:Sheep / Q000276:immunology
D000178:Acrylamides; D005656:Fungal Proteins; D007369:Interferon Inducers; D020106:Acrylamide; C008524:MFA 6 extract, Aspergillus ochraceus