Host cell membrane proteins on human immunodeficiency virus type 1 after in vitro infection of H9 cells and blood mononuclear cells. An immuno-electron microscopic study.
Human immunodeficiency virus type 1 (HIV-1)-infected H9 and blood mononuclear cells (MNCs) were studied by immunogold electron microscopy for the presence of HIV-1 gag p24 protein, env gp41 and gp120 proteins, and host cell molecules CD4, CD11a, CD25, CD54, CD63, HLA class I and HLA-DR. Uninfected H9 cells and MNC membranes labelled for CD4, HLA class I and class II, and, at low density, CD11a and CD54; lysosomal structures in the cytoplasm labelled for CD63. The infected cell surface showed immunolabelling for HIV-1 proteins, as did budding particle-like structures. Immunogold labelling of the cell membrane for CD4 was almost non-existent. The level of immunolabelling for CD11a and CD54 on infected cells was greater than that on uninfected cells; this is presumably related to a state of activation during virus synthesis. Budding particle-like structures and free virions in the intercellular space were immunogold-labelled for all host cell markers investigated. This was confirmed by double immunogold labelling using combinations of HIV-1 gag p24 labelling and labelling for the respective host cell molecule. We conclude that virions generated in HIV-1-infected cells concentrate host-derived molecules on their envelope. Also molecules with a prime function in cellular adhesion concentrate on the virion.
Meerloo
T
T
Department of Pathology, University Hospital, Utrecht, The Netherlands.
Sheikh
M A
MA
Bloem
A C
AC
de Ronde
A
A
Schutten
M
M
van Els
C A
CA
Roholl
P J
PJ
Joling
P
P
Goudsmit
J
J
Schuurman
H J
HJ
D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D018845:CD11 Antigens; D015815:Cell Adhesion Molecules / Q000032:analysis; D002460:Cell Line; D016655:HIV Core Protein p24 / Q000032:analysis; D015699:HIV Envelope Protein gp120 / Q000032:analysis; D015700:HIV Envelope Protein gp41 / Q000032:analysis; D015497:HIV-1 / Q000737:chemistry*; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D008565:Membrane Proteins / Q000032:analysis*; D016253:Microscopy, Immunoelectron
D015703:Antigens, CD; D000954:Antigens, Surface; D018845:CD11 Antigens; D015815:Cell Adhesion Molecules; D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D006680:HLA Antigens; D008565:Membrane Proteins; D018799:Intercellular Adhesion Molecule-1