Multicenter evaluation of ATB fungus: a standardized micromethod for yeast susceptibility testing.
The micromethod for yeast susceptibility testing, ATB Fungus, was evaluated with 30 reference strains in three laboratories. Ready-to-use strips with 5-fluorocytosine, amphotericin B, nystatin, miconazole, econazole and ketoconazole were used. The test allowed the categorization of each strain as susceptible, intermediate or resistance to all the antifungals tested, and 5-fluorocytosine and amphotericin B MIC determination. The results were compared with the MIC for each reference strain obtained by a microdilution method on RPMI 1640 buffered with MOPS. The repeatability and intralaboratory and interlaboratory reproducibility were evaluated. ATB Fungus was a reliable and reproducible method with a repeatability of 96.6%, a reproducibility of 95.4% and showed an excellent correlation 91.7%) with reference MICs.
Quindós
G
G
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina, Universidad del País Vasco, Bilbao, España.
Salesa
R
R
Carrillo-Muñoz
A J
AJ
Lipperheide
V
V
Jáudenes
L
L
San Millán
R
R
Torres-Rodríguez
J M
JM
Pontón
J
J
D000935:Antifungal Agents / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013045:Species Specificity; D015003:Yeasts / Q000187:drug effects*
D000935:Antifungal Agents; D011933:Reagent Kits, Diagnostic