Analysis of the cytosolic hsp70 gene family in Zea mays.
In this study we have analysed the multigene family coding for the cytoplasmic heat shock 70 kDa proteins (hsp70) in Zea mays. Fully degenerate primers were used in a polymerase chain reaction (PCR) to amplify selected regions of the hsp70 genes. Sequence and Southern blot analysis reveals that at least three highly conserved genes exist in maize. In addition, amplification reveals the presence of a conserved intron in all genes examined. Expression analysis shows that the hsp70 genes studied represent members of the inducible and constitutive families. The results obtained may indicate that there are subfamilies of cytoplasmic hsp70 genes expressed in higher plants.
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Ecole Normale Supérieure de Lyon, UMR 9938, CNRS-INRA, France.
Vergne
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Dumas
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000502:physiology*; D005786:Gene Expression Regulation; D017343:Genes, Plant / Q000235:genetics*; D005838:Genotype; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D010446:Peptide Fragments / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D003313:Zea mays / Q000235:genetics*
D006360:Heat-Shock Proteins; D010446:Peptide Fragments