title: A developmental decrease in NMDA-mediated spontaneous firing in cultured rat cerebral cortex.
abstract: In primary cultures of fetal rat cerebral cortex chronic manipulation of the level and/or pattern of bioelectric activity leads to plastic changes in bioelectric activity, opposite to those seen during the manipulation. This suggests the presence of adaptive mechanisms which regulate functional development in the neuronal network. Since NMDA receptors play an important role in early postnatal bioelectric activity and have been implicated in activity-dependent plasticity in vivo, the involvement of NMDA and non-NMDA receptors in spontaneously occurring bioelectric activity was investigated in cultured rat cerebral cortex by assaying the effects of NMDA and non-NMDA antagonists on neuronal firing. In addition, the physiological consequences of chronic suppression of bioelectric activity were investigated following development in the presence of tetrodotoxin. NMDA receptors appeared at all ages to be more crucial for spontaneous bioelectric activity than non-NMDA receptors, although their relative importance decreased during the first 3 weeks. Whereas the NMDA antagonist APV strongly reduced burst firing, the non-NMDA antagonist DNQX tended to increase burst firing slightly. Following chronic suppression of bioelectric activity, non-variable burst firing was increased, thus replicating previous findings in cerebral cortex culture grown under different conditions. The prominence of NMDA receptor activation in spontaneous bioelectric activity in early cultures suggests a role for these receptors in activity-dependent functional plasticity, as found in vivo.
authors.affiliation: Netherlands Institute for Brain Research, Amsterdam.
authors.forename: G J
authors.initials: GJ
authors.lastname: Ramakers
authors.forename: C
authors.initials: C
authors.lastname: de Wit
authors.forename: P S
authors.initials: PS
authors.lastname: Wolters
authors.forename: M A
authors.initials: MA
authors.lastname: Corner
mesh_terms: D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000254:growth & development* / Q000502:physiology; D003470:Culture Media; D004594:Electrophysiology; D005260:Female; D016202:N-Methylaspartate / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D011247:Pregnancy; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000187:drug effects; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D011810:Quinoxalines; D016194:Receptors, N-Methyl-D-Aspartate; D013779:Tetrodotoxin; C056723:FG 9041; D016202:N-Methylaspartate; D015763:2-Amino-5-phosphonovalerate