The effect of vpu on HIV-1-induced syncytia formation.
To investigate the role of vpu in the cytopathicity of human immunodeficiency type 1 (HIV-1), the MT4 CD4+ T-cell line was infected with viruses that were isogenic except for their ability to produce the vpu protein. The experiments described here demonstrate that expression of vpu reduces HIV-1 cytopathic effects by decreasing the rate of syncytia formation. By reducing the concentration of gp 120 at the cell surface, vpu limits cell killing by syncytia formation.
Yao
X J
XJ
Département de Microbiologie et Immunologie, Faculté de Médecine, Université de Montréal, Quebec, Canada.
Garzon
S
S
Boisvert
F
F
Haseltine
W A
WA
Cohen
E A
EA
D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism / Q000382:microbiology*; D002213:Capsid / Q000096:biosynthesis; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000235:genetics; D016338:Genes, vpu; D015726:Giant Cells / Q000648:ultrastructure; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015699:HIV Envelope Protein gp120 / Q000096:biosynthesis; D015497:HIV-1 / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D054298:Human Immunodeficiency Virus Proteins; D006801:Humans; D008854:Microscopy, Electron; D054334:Viral Regulatory and Accessory Proteins / Q000096:biosynthesis / Q000502:physiology*; D014771:Virion / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D000086082:Viroporin Proteins
D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120; D054298:Human Immunodeficiency Virus Proteins; D054334:Viral Regulatory and Accessory Proteins; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins