Developmentally ordered appearance of a functional Fc gamma receptor on TCR V gamma 3 thymocytes. Evidence for stimulation-induced expression.
In the present report, we show that the Fc gamma receptor (Fc gamma R) becomes expressed on mature TCR V gamma 3 thymocytes, but it is absent on immature TCR V gamma 3 cells. Both Fc gamma RIII are Fc gamma RIII are expressed. The in vivo expression of the Fc gamma R on mature TCR V gamma 3 thymocytes coincides with an activated phenotype of the cells. In vitro, anti-CD3 stimulation of Fc gamma R-negative, immature TCR V gamma 3 thymocytes results in the expression of the Fc gamma R. The Fc gamma R of short-term IL-2-cultured, mature TCR V gamma 3 thymocytes is functional in an Ab-mediated cytotoxicity assay. In addition, it is shown that cross-linking of the Fc gamma R induces an increase in cytoplasmic Ca2+. Collectively, our findings show that mature TCR V gamma 3 thymocytes express a functional Fc gamma R; evidence is presented that this could be a result of in vivo activation of these cells.
Leclercq
G
G
Department of Clinical Chemistry, Microbiology, and Immunology, University of Ghent, University Hospital, Belgium.
Plum
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D015139:Blotting, Southern; D002118:Calcium / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D005314:Embryonic and Fetal Development / Q000276:immunology; D005434:Flow Cytometry; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000502:physiology*; D017452:Receptors, IgG / Q000096:biosynthesis*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology* / Q000196:embryology
D000911:Antibodies, Monoclonal; D016692:Receptors, Antigen, T-Cell, gamma-delta; D017452:Receptors, IgG; D002118:Calcium