Effect of cumulus cells and exposure period to ethanol on in vitro development of mouse diploid parthenogenones.
Cumulus-intact and cumulus-free mouse oocytes were exposed to 7% ethanol for 1, 4 and 7 min, and treated with cytochalasin-B. The activation rate and the proportion of diploid parthenogenones in all groups were not significantly different. After 96 hr in culture, a higher number of blastocysts was obtained when either cumulus-intact or cumulus-free oocytes were exposed for shorter times (1 and 4 min) to ethanol. The presence or absence of cumulus cells at activation had no effect on the percentage of blastocysts. However, at 1 and 4 min ethanol-exposure periods, the parthenogenones derived from cumulus-intact oocytes had a higher number of cells than ones derived from cumulus-free oocytes.
Pinyopummin
A
A
Department of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Takahashi
Y
Y
Cheong
H T
HT
Hishinuma
M
M
Kanagawa
H
H
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D004171:Diploidy; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010312:Parthenogenesis; D013480:Superovulation
D000431:Ethanol