Inhibition of NGF-induced neurite outgrowth of PC12 cells by Bacillus cereus sphingomyelinase, a bacterial hemolysin.
Sphingomyelinase of Bacillus cereus, a bacterial hemolysin, reduced nerve growth factor (NGF)-induced neurite outgrowth of PC12 cells in a dose-dependent manner. At 200 mU/ml, sphingomyelinase repressed half the neurite outgrowth of the cells at 250 ng/ml NGF. The c-fos superinduction, one of the early responses induced by NGF, was not influenced by this treatment, suggesting that the repression by sphingomyelinase occurred via a protein kinase C-independent pathway.
Tamura
H
H
Department of Microbial Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Noto
M
M
Kinoshita
K
K
Ohkuma
S
S
Ikezawa
H
H
D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology*; D002454:Cell Differentiation / Q000187:drug effects; D009414:Nerve Growth Factors / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells; D010673:Pheochromocytoma; D051381:Rats; D013108:Sphingomyelin Phosphodiesterase / Q000494:pharmacology*
D009414:Nerve Growth Factors; D013108:Sphingomyelin Phosphodiesterase