title: The zymogen of the protease that degrades small, acid-soluble proteins of spores of Bacillus species can rapidly autoprocess to the active enzyme in vitro.
abstract: The zymogen of the protease (GPR) that initiates protein degradation during spore germination in Bacillus species is not activated in vitro under normal physiological conditions. However, there is rapid, acid-pH-dependent, zero-order, proteolytic activation of the purified zymogen in high concentrations of dimethyl sulfoxide. These findings provide further evidence that GPR activates itself during sporulation.
authors.affiliation: Department of Biochemistry, University of Connecticut Health Center, Farmington 06030.
authors.forename: B
authors.initials: B
authors.lastname: Illades-Aguiar
authors.forename: P
authors.initials: P
authors.lastname: Setlow
mesh_terms: D001407:Bacillus / Q000201:enzymology / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D010450:Endopeptidases / Q000096:biosynthesis / Q000378:metabolism*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism*; D007700:Kinetics; D011499:Protein Processing, Post-Translational; D013171:Spores, Bacterial / Q000502:physiology; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D004792:Enzyme Precursors; D010450:Endopeptidases