title: Secretin inhibits induction of ornithine decarboxylase activity by gastrin in duodenal mucosa and IEC-6 cells.
abstract: Ornithine decarboxylase (ODC) catalyzes the first rate-limiting step in polyamine biosynthesis, and increased ODC activity is one of the earliest biochemical events associated with the induction of cellular proliferation. The current study examines the regulation of ODC activity in rat duodenal mucosa and IEC-6 cells (a line of normal rat intestinal crypt cells) in response to the trophic hormone, gastrin, and its inhibitor, secretin. Rats were fasted 22 h before the various treatments, and ODC activity was measured in scraped duodenal mucosa. Gastrin significantly increased ODC activity within 3 h to 4.3 times control levels. The effect of gastrin was totally inhibited by 5 micrograms/kg secretin. In doses of 5 or 10 micrograms/kg, secretin had no effect on basal ODC. Epidermal growth factor (EGF) and refeeding fasted rats also significantly increased ODC activity in duodenal mucosa, but the effects of EGF and refeeding were not prevented by secretin. In cultured IEC-6 cells, ODC activity was significantly increased after exposure to gastrin, 5% dialyzed fetal bovine serum (FBS), EGF, and asparagine. Secretin in doses ranging from 10(-10) to 10(-6) M caused a linear and significant inhibition of the stimulation of ODC activity by gastrin. No dose of secretin affected basal ODC activity or enzyme activity stimulated by 5% dialyzed FBS, EGF, or asparagine in IEC-6 cells. The ODC mRNA levels in IEC-6 cells were also increased after exposure to gastrin. Administration of secretin significantly prevented the stimulated expression of the ODC gene in cells treated with gastrin.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology and Biophysics, University of Tennessee College of Medicine, Memphis 38163.
authors.forename: J Y
authors.initials: JY
authors.lastname: Wang
authors.forename: S A
authors.initials: SA
authors.lastname: McCormack
authors.forename: M J
authors.initials: MJ
authors.lastname: Viar
authors.forename: L R
authors.initials: LR
authors.lastname: Johnson
mesh_terms: D000818:Animals; D002460:Cell Line; D004386:Duodenum / Q000201:enzymology*; D004790:Enzyme Induction / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005215:Fasting; D005502:Food; D005755:Gastrins / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D007413:Intestinal Mucosa / Q000201:enzymology*; D007422:Intestines / Q000166:cytology / Q000201:enzymology*; D008297:Male; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism; D065108:Ornithine Decarboxylase Inhibitors; D010418:Pentagastrin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012633:Secretin / Q000494:pharmacology*
chemical_list: D005755:Gastrins; D065108:Ornithine Decarboxylase Inhibitors; D012633:Secretin; D004815:Epidermal Growth Factor; D009955:Ornithine Decarboxylase; D010418:Pentagastrin