title: Inhibition of herpes simplex virus 1 reproduction with hydrophobized antisense oligonucleotides.
abstract: Antisense oligonucleotides were modified by their ends with hydrophobic substituents which permitted enhancing their activity. The effect of hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus type 1 in Vero cells was studied. Two types of oligonucleotides were used: a 12-mer complementary to the splicing site 983-994 of early mRNA-5 of the virus and 19-mer complementary to the site of mRNA that encodes the virus DNA-polymerase. These oligonucleotides were modified by 5'-ends with n-undecyl or cholesteryl moieties and by 3'-ends with acridine or 1,7-heptanediol. In comparison with the unmodified oligonucleotides the hydrophobized ones were significantly more active and inhibited HSV-1 infection at micromolar concentrations.
authors.affiliation: Moscow Institute of Biotechnology Inc., Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Vinogradov
authors.forename: Y
authors.initials: Y
authors.lastname: Suzdaltseva
authors.lastname: Alakhov VYu
authors.forename: A V
authors.initials: AV
authors.lastname: Kabanov
mesh_terms: D000166:Acridines / Q000737:chemistry; D000818:Animals; D001483:Base Sequence; D002784:Cholesterol / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D006018:Glycols / Q000737:chemistry; D015497:HIV-1 / Q000187:drug effects / Q000254:growth & development*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D012326:RNA Splicing; D012333:RNA, Messenger; D012367:RNA, Viral; D014709:Vero Cells
chemical_list: D000166:Acridines; D006018:Glycols; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D012367:RNA, Viral; C007927:1,7-heptanediol; D002784:Cholesterol; D004259:DNA-Directed DNA Polymerase