title: Construction of immunoradiometric assay for circulating c-erbB-2 protooncogene product in advanced breast cancer patients.
abstract: The human c-erbB-2 protooncogene product (erbB-2 protein) is a 185 kilodalton glycoprotein closely related to epidermal growth factor receptor protein. In this study, we measured the concentration of circulating erbB-2 protein in cancer patients by means of a new immunoradiometric assay (IRMA). Two monoclonal antibodies (MoAbs), SV2-61 gamma and 6G10, recognize erbB-2 protein but bind to separate epitopes. SV2-61 gamma was used as an immunoadsorbent and 6G10 as an 125I-labeled probe. A serum was considered positive for erbB-2 protein if the percent binding exceeded the mean of the normal group by more than 3 standard deviations. Eleven of 21 patients with advanced breast cancer and 1 of 15 with advanced gastric cancer were positive. Serum erbB-2 protein levels correlated well with the therapy and the status of the patients with breast cancer. On the contrary, all patients with advanced colon, ovarian, or pancreatic cancers, showed levels below the cut-off value. These results suggest that circulating erbB-2 protein can be measured using the newly constructed IRMA. Since c-erbB-2 protooncogene amplification and overexpression are accepted as a good marker of aggressiveness, relapsing potency, and poor prognosis, this IRMA should be a promising tool with which to help manage breast cancer patients.
authors.affiliation: Department of Nuclear Medicine, Kyoto University Hospital, Shogoin.
authors.forename: M
authors.initials: M
authors.lastname: Hosono
authors.forename: T
authors.initials: T
authors.lastname: Saga
authors.forename: H
authors.initials: H
authors.lastname: Sakahara
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Shirato
authors.forename: K
authors.initials: K
authors.lastname: Endo
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Akiyama
authors.forename: K
authors.initials: K
authors.lastname: Toyoshima
authors.forename: J
authors.initials: J
authors.lastname: Konishi
mesh_terms: D000230:Adenocarcinoma / Q000097:blood*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood; D001943:Breast Neoplasms / Q000097:blood*; D003110:Colonic Neoplasms / Q000097:blood; D005260:Female; D006801:Humans; D015592:Immunoradiometric Assay; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood; D010190:Pancreatic Neoplasms / Q000097:blood; D011518:Proto-Oncogene Proteins / Q000097:blood*; D018719:Erb-b2 Receptor Tyrosine Kinases; D013274:Stomach Neoplasms / Q000097:blood
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases