Extraction and liquid chromatographic method for the determination of simmondsin in plasma.
A solid-phase method for the extraction of simmondsin from plasma was developed along with a liquid chromatographic method for the quantitative measurement of this compound. The extraction of simmondsin is realised with activated carbon. Chromatography is performed on a 25 x 0.46 cm I.D. RP-18, 5-micron HPLC column with a water-methanol (85:15, v/v) mobile phase and ultraviolet absorbance detection at 217 nm. The limit of detection is 100 ng (using 1 ml of plasma). The linear quantitation range is 0.1-200 micrograms/ml.
Van Boven
M
M
Katholieke Universiteit Leuven, Laboratory of Toxicology, Belgium.
Daenens
P
P
Cokelaere
M
M
Decuypere
E
E
D000097:Acetonitriles / Q000097:blood*; D001798:Blood Proteins; D002244:Carbon; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D003510:Cyclohexanes; D005960:Glucosides / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000097:Acetonitriles; D001798:Blood Proteins; D003510:Cyclohexanes; D005960:Glucosides; D002244:Carbon; C089377:simmondsin; C032159:acetonitrile