NMDA-sensitive [3H]glutamate binding in the epileptic rat hippocampus: an autoradiographic study.
Sustained stimulation of the perforant pathway (PP) offers a model for studying seizure-induced pathology of the hippocampal formation. Using quantitative autoradiography, NMDA-sensitive glutamate binding was evaluated in the rat hippocampus three weeks after PP-stimulation. Decreased receptor binding (38-44%) was found together with pyramidal cell damage and gliosis in the CA1 area, ipsilateral to the stimulation (p < or = 0.001). In the dentate gyrus, however, a 20% increase in receptor density was observed bilaterally within the molecular layer (significant within the outer molecular layer of the contralateral side, on the suprapyramidal location; p < or = 0.05). These results are similar to those found in epileptic patients and suggest a prominent role for NMDA receptors in epilepsy.
Lahtinen
H
H
Department of Neurology, University of Kuopio, Finland.
Castrén
E
E
Miettinen
R
R
Ylinen
A
A
Paljärvi
L
L
Riekkinen
P J
PJ
D000818:Animals; D001345:Autoradiography; D016923:Cell Death / Q000187:drug effects; D004569:Electroencephalography; D004827:Epilepsy / Q000378:metabolism* / Q000473:pathology; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D006651:Histocytochemistry; D007700:Kinetics; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism
D005971:Glutamates; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D016202:N-Methylaspartate