Differentiation-dependent expression of sialyl stage-specific embryonic antigen-1 and I-antigens on human lymphoid cells and its implications for carbohydrate-mediated adhesion to vascular endothelium.
Expression of two developmentally regulated carbohydrate antigens, the sialyl stage-specific embryonic antigen-1 (SSEA-1) and I-antigens, in human lymphocytes and lymphocytic leukemia cells was investigated using specific monoclonal antibodies. Sialyl SSEA-1 was expressed only on natural killer (NK) cells, and was essentially absent on resting mature T and B cells among normal peripheral lymphocytes. On the other hand, the I-antigen was strongly expressed on virtually all mature B cells, moderately expressed on most mature T cells, but not expressed on NK cells in normal donors. Expression of the two antigens on normal T and B cells was reversible; in vitro stimulation of normal lymphocytes with concanavalin A (Con A) resulted in the loss of I-antigen and appearance of sialyl SSEA-1 on CD3+ T blasts, whereas stimulation with pokeweed mitogen led to loss of I-antigen expression and appearance of sialyl SSEA-1 antigen on CD19+ B blasts. Among lymphocytic leukemia cells, sialyl SSEA-1 was detected primarily on leukemia cells having immature properties such as most common acute lymphocytic leukemia (cALL) blasts, while the I-antigen was frequently expressed on malignant cells having relatively mature properties, such as those found in adult T-cell leukemia or chronic lymphocytic leukemia, and only occasionally on cALL blasts. Among normal peripheral lymphocytes, the sialyl SSEA-1+I-antigen- NK cells selectively underwent E-selectin (ELAM-1, endothelial-leukocyte adhesion molecule-1)-dependent adhesion to endothelial cells, while the I-antigen+sialyl SSEA-1- mature T and B cells did not, in line with the recent finding that sialyl SSEA-1 serves as a specific ligand for E-selectin. Con A blasts, which are sialyl SSEA-1+I-antigen-, also exhibited significant E-selectin-dependent adhesion to endothelial cells. These results indicate that expression of the sialyl SSEA-1 and I-antigens varies alternately depending on the differentiation/activation status of the lymphocytes, and that this at least partly regulates the behavior of lymphocytes at the vessel wall.
Ohmori
K
K
Department of Laboratory Medicine, Kyoto University, School of Medicine, Japan.
Takada
A
A
Yoneda
T
T
Buma
Y
Y
Hirashima
K
K
Tsuyuoka
K
K
Hasegawa
A
A
Kannagi
R
R
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000502:physiology*; D006028:Glycosphingolipids / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000276:immunology; D016256:Lewis X Antigen / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000502:physiology; D008969:Molecular Sequence Data; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D006028:Glycosphingolipids; C078965:I-antigen; D016256:Lewis X Antigen; D011043:Pokeweed Mitogens; D003208:Concanavalin A