Identification of a TXREB pseudogene (TXREBP) located between the genes for p55 (MPP1) and G6PD on Xq28.
A fibroblast cDNA library was screened by hybridization to a yeast artificial chromosome containing genomic sequences from human Xq28. The majority of positive cDNA clones were found to correspond to the cDNA coding for TXREB, an HTLV-1 enhancer-binding protein. Sequence analysis of the Xq28 genomic DNA revealed a number of deleterious changes compared to the previously reported cDNA. In addition, both the genomic DNA and cDNA isolates were found to be lacking a 599-bp sequence, bracketed by GT and AG, in the 5' untranslated region. These results suggest that the Xq28-linked gene is a processed pseudogene for TXREB and that the previously reported cDNA was only partially processed. Southern blot analysis on a hybrid mapping panel confirmed the presence of at least one autosomal gene for TXREB, and Northern blot hybridization with the 599-bp putative intron probe confirmed that the sequence is not part of the mature mRNA. Further analysis showed that the gene is expressed in a variety of human tissues and that the pseudogene is located between the genes for the proteins p55 and G6PD.
Das
S
S
Department of Medicine, University of California, San Francisco 94143-0724.
Gitschier
J
J
D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D015152:Blotting, Northern; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005347:Fibroblasts; D016356:Gene Products, tax / Q000235:genetics*; D005796:Genes; D016355:Genes, pX; D005819:Genetic Markers; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011544:Pseudogenes; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome
D001798:Blood Proteins; D015342:DNA Probes; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D016356:Gene Products, tax; D005819:Genetic Markers; C089465:MPP1 protein, human; D008565:Membrane Proteins; D005954:Glucosephosphate Dehydrogenase