title: Extraction and liquid chromatographic method for the determination of simmondsin in plasma.
abstract: A solid-phase method for the extraction of simmondsin from plasma was developed along with a liquid chromatographic method for the quantitative measurement of this compound. The extraction of simmondsin is realised with activated carbon. Chromatography is performed on a 25 x 0.46 cm I.D. RP-18, 5-micron HPLC column with a water-methanol (85:15, v/v) mobile phase and ultraviolet absorbance detection at 217 nm. The limit of detection is 100 ng (using 1 ml of plasma). The linear quantitation range is 0.1-200 micrograms/ml.
authors.affiliation: Katholieke Universiteit Leuven, Laboratory of Toxicology, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Van Boven
authors.forename: P
authors.initials: P
authors.lastname: Daenens
authors.forename: M
authors.initials: M
authors.lastname: Cokelaere
authors.forename: E
authors.initials: E
authors.lastname: Decuypere
mesh_terms: D000097:Acetonitriles / Q000097:blood*; D001798:Blood Proteins; D002244:Carbon; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D003510:Cyclohexanes; D005960:Glucosides / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000097:Acetonitriles; D001798:Blood Proteins; D003510:Cyclohexanes; D005960:Glucosides; D002244:Carbon; C089377:simmondsin; C032159:acetonitrile