Evaluation of commercial amplification kit for detection of Legionella pneumophila in clinical specimens.
A commercial kit (EnviroAmp) designed to detect the DNA of Legionella species in environmental water samples using PCR and reverse dot hybridization was applied to clinical specimens. Results correlated well with culture for bronchoalveolar lavages. In addition, this test was easy to perform and showed good sensitivity.
Matsiota-Bernard
P
P
Laboratoire de Microbiologie, Hôpital Raymond Poincaré, Faculté de Médecine Paris-Ouest, Garches, France.
Pitsouni
E
E
Legakis
N
N
Nauciel
C
C
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D016952:Legionella pneumophila / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011933:Reagent Kits, Diagnostic