title: Actin-binding protein, drebrin, accumulates in submembranous regions in parallel with neuronal differentiation.
abstract: Drebrins are developmentally regulated actin-binding proteins. In this study, we analyzed subcellular distribution of drebrin E in neuroblastoma cells (SH-SY5Y) in culture, especially in terms of its relationship to actin filaments. In undifferentiated cells, drebrin E was scattered as flocculus small dots along the stress fibers and also accumulated at adhesion plaques. In parallel with the neuronal differentiation following retinoic acid treatment, drebrin E was accumulated, accompanying filamentous (F) actin, in the submembranous cortical cytoplasm. Similar submembranous localization of drebrins was observed in primary cultured neurons. In the presence of drebrin E F-actin was more stable against cytochalasin D than F-actin lacking drebrin E. These results suggest that drebrin E plays a role in neuronal morphological differentiation by changing its subcellular localization with stabilized F-actin.
authors.affiliation: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Asada
authors.forename: K
authors.initials: K
authors.lastname: Uyemura
authors.forename: T
authors.initials: T
authors.lastname: Shirao
mesh_terms: D000199:Actins / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D015638:Cytochalasin D / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D009447:Neuroblastoma / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009479:Neuropeptides / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D009479:Neuropeptides; C053386:drebrins; D015638:Cytochalasin D; D014212:Tretinoin