title: Transfection of the bcr/abl oncogene into factor-dependent cells by electroporation: acquisition of autonomous proliferation.
abstract: In order to clarify the function of P210 bcr/abl oncogene in leukemogenesis, IL-3 dependent murine hematopietic cell line, FDC-P2, was transfected with the plasmid containing cDNA of P210 bcr/abl oncogene (pGD'210) or murine IL-3 (pcDmIL3) by electroporation. Four out of five pGDH210 transfected clones as well as FDC-P2 transfected with pcDmIL3, acquired autonomous proliferation (i.e. lost the requirement for IL-3 supplementation). The expression of bcr/abl oncogene was weak in one clone, which remained dependent on IL-3. Unlike pcDmIL3 transfectants, which secrete IL-3 into the supernatant, IL-3 was not demonstrated in the culture supernatant of pGD'210 transfected FDC-P2. These finding suggest that P210 bcr/abl oncogene is directly associated with autonomous proliferation, which is the first process of leukemogenesis.
authors.affiliation: First Department of Internal Medicine, Niigata University, School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Furukawa
authors.forename: I
authors.initials: I
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Ohsawa
authors.forename: K
authors.initials: K
authors.lastname: Nikkuni
authors.forename: A
authors.initials: A
authors.lastname: Oaki
authors.forename: T
authors.initials: T
authors.lastname: Goto
authors.forename: T
authors.initials: T
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Kishi
authors.forename: T
authors.initials: T
authors.lastname: Koike
mesh_terms: D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D017077:Culture Media, Conditioned; D017931:DNA Primers; D018274:Electroporation / Q000379:methods; D016044:Fusion Proteins, bcr-abl / Q000096:biosynthesis*; D016313:Genes, abl; D006412:Hematopoietic Stem Cells; D007377:Interleukin-3 / Q000096:biosynthesis; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014162:Transfection
chemical_list: D017077:Culture Media, Conditioned; D017931:DNA Primers; D007377:Interleukin-3; D016044:Fusion Proteins, bcr-abl