[The analysis of human DNA fingerprints by the synthetic oligonucleotide probe].
In order to test the practical applicability of oligonucleotide fingerprinting we have investigated 50 unrelated individuals, 11 members in two families and one pair of twin using the probe (CAC)5/(GTG)5. Except the monozygotic twins highly variable patterns were demonstrated, even in a family. The probability of chance association between random individuals was calculated as 3.8 x 10(-10). It seems reasonable to conclude that (CAC)5/(GTG)5 fingerprints are completely individual-specific. The results of the pedigree study showed that the inheritance of alleles was in keeping with the Mendelian Law. This method is not so costly and labor-intensive as the conventional Southern blotting. It increases the speed and lessen the complexity of RFLPs study.
Lan
L
L
Beijing Forensic Science Institute.
Zhang
X
X
Huo
Z
Z
Duan
B
B
D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D015345:Oligonucleotide Probes; D012150:Polymorphism, Restriction Fragment Length
D015345:Oligonucleotide Probes