title: Immunohistochemical localization of chromogranin A and B in the endocrine cells of the alimentary tract of the green frog, Rana esculenta.
abstract: Novel monoclonal antibodies to human chromogranin A (CgA) and chromogranin B (CgB) were used to investigate the presence of immunoreactive (-IR) elements in the alimentary tract of the green frog Rana esculenta. Numerous CgA-IR and a few CgB-IR endocrine cells were found within the gut mucosa, from the oesophagus to the cloaca, with some local differences in density. Co-localization studies demonstrated that they were co-stored in almost all the serotonin-IR, the amylin-IR or islet amyloid polypeptide-IR cells and in the peptide tyrosine tyrosine-IR cells located proximal to the pylorus, but not in those located in more caudal tracts. No other co-localization was demonstrated; substances investigated included somatostatin, substance P, gastrin/cholecystokinin, glucagon, glycentin, bombesin, secretin and neurotensin. CgA-IR and CgB-IR cells nearly always displayed argyrophilia with the Grimelius silver method.
authors.affiliation: Institute of Human Anatomy, University La Sapienza, Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: D'Este
authors.forename: R
authors.initials: R
authors.lastname: Buffa
authors.forename: M
authors.initials: M
authors.lastname: Pelagi
authors.forename: A G
authors.initials: AG
authors.lastname: Siccardi
authors.forename: T
authors.initials: T
authors.lastname: Renda
mesh_terms: D000682:Amyloid / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D053379:Chromogranin A; D002864:Chromogranins / Q000276:immunology / Q000378:metabolism*; D004064:Digestive System / Q000166:cytology / Q000378:metabolism*; D004702:Endocrine Glands / Q000166:cytology / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D058228:Islet Amyloid Polypeptide; D008297:Male; D019894:Peptide YY; D010455:Peptides / Q000378:metabolism; D011893:Rana esculenta; D012701:Serotonin / Q000378:metabolism
chemical_list: D000682:Amyloid; D000911:Antibodies, Monoclonal; C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D058228:Islet Amyloid Polypeptide; D010455:Peptides; D019894:Peptide YY; D012701:Serotonin