A method based on PCR for the construction of cDNA libraries and probes from small amounts of tissue.
A PCR-based method is described for the production of cDNA libraries and total cDNA probes from a few milligrams of tissue. Using a model system, we show how a PCR library and PCR probes can be used to identify genes expressed at different levels in two tissues. Small amounts of tissue derived from two plants, one infected with arabis mosaic virus and the other uninfected, were used to make a library and probes. This library and the probes were used to identify viral genes expressed only in the infected plant.
Bertiol
D J
DJ
AFRC-IACR, Rothamsted Experimental Station Harpenden, Herts, UK.
Smoker
M
M
Brown
A C
AC
Jones
M G
MG
Burrows
P R
PR
D001483:Base Sequence; D015342:DNA Probes / Q000096:biosynthesis*; D018076:DNA, Complementary / Q000096:biosynthesis*; D015723:Gene Library; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics / Q000382:microbiology; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D018076:DNA, Complementary