Assessment of sister chromatid exchange in mouse embryos produced by microsurgical fertilization.
The treatment of male factor-related infertility has been approached with the advent of several methods for microsurgical fertilization, such as the partial dissection of the zona pellucida (PZD) and the injection of sperm into the perivitelline space (PVSI) of oocytes. These techniques are designed to increase sperm-oolemma interaction by circumventing passage of the sperm through the zona pellucida. The present study was performed to evaluate the influence of PZD and PVSI on the in vitro development of mouse embryos by assessing the rate of sister chromatid exchange (SCE). SCE is considered to be a sensitive indirect indicator of DNA lesions due to various conditions. Oocytes were cultured in vitro after PZD or PVSI and then examined for SCE. There was no significant difference in SCE between control and treatment groups of embryos and the values were similar to those reported by Saito et al. (Fertil Steril 41:460-464, 1984). The rate of SCE was low during the first two mitotic cycles, then increased from cycle two to three before declining slightly between the 3rd and 4th cycles of cell division. These data demonstrate that the direct interaction of sperm and oocyte by PZD or PVSI did not have an adverse effect on the development of mouse embryos as assessed by the rate of SCE.
Hirayama
T
T
Institute for Reproductive Research, Los Angeles, California.
Quinn
P
P
Marrs
R P
RP
D000818:Animals; D004622:Embryo, Mammalian / Q000648:ultrastructure*; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D008297:Male; D051379:Mice; D008845:Microinjections; D008866:Microsurgery / Q000379:methods; D008938:Mitosis; D012854:Sister Chromatid Exchange; D013084:Sperm-Ovum Interactions; D014817:Vitelline Membrane / Q000601:surgery; D015044:Zona Pellucida / Q000648:ultrastructure