title: Increased viral burden and cytopathicity correlate temporally with CD4+ T-lymphocyte decline and clinical progression in human immunodeficiency virus type 1-infected individuals.
abstract: The rate of clinical progression is variable among individuals infected with human immunodeficiency virus type 1 (HIV-1). Changes in viral burden which correlate with disease status have been demonstrated in cross-sectional studies; however, a detailed longitudinal study of the temporal relationship between viral burden, CD4+ T-cell numbers, and clinical status throughout the course of infection has not been reported. Multiple longitudinal blood samples were obtained from four HIV-1-infected individuals with clinically divergent profiles. Levels of HIV-1 were measured in sequential samples of peripheral blood mononuclear cells, using both end-point dilution cultures and quantitative polymerase chain reaction methods. Serial HIV-1 isolates from each case were also evaluated to determine their biological properties in vitro. For the three patients with clinical progression, a dramatic increase in the level of HIV-1 was observed concurrent with or prior to a marked drop in CD4+ T lymphocytes. This increase in viral burden was temporally associated with the emergence of a more cytopathic viral phenotype. In contrast, consistently low levels of HIV-1 were observed in the one patient who was clinically and immunologically stable for more than a decade. Moreover, viral isolates from this patient were less cytopathic in vitro compared with HIV-1 isolates from those patients with disease progression. The temporal association between increased viral burden and CD4+ T-cell decline suggests a direct role for HIV-1 in the cytopathology of CD4+ T cells in vivo. Our results indicate that the pathogenic mechanisms responsible for CD4+ T-cell depletion may be related to both quantitative and qualitative changes in HIV-1.
authors.affiliation: Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016.
authors.forename: R I
authors.initials: RI
authors.lastname: Connor
authors.forename: H
authors.initials: H
authors.lastname: Mohri
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.forename: D D
authors.initials: DD
authors.lastname: Ho
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology* / Q000473:pathology*; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D003588:Cytopathogenic Effect, Viral; D015497:HIV-1 / Q000254:growth & development* / Q000472:pathogenicity; D006716:Homosexuality; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D013997:Time Factors; D014779:Virus Replication
chemical_list: D009838:Oligodeoxyribonucleotides