Methylglyoxal and cell viability.
Methylglyoxal by depleting glutathione stores increased Trypan-blue uptake by the cells incubated in glucose, pyruvate and amino acids free medium. Only a transient fall of glutathione concentration without any effect on cell viability was caused by methylglyoxal when the medium was supplemented with above-mentioned compounds. The role of gamma-glutamyl-transpeptidase is discussed.
Braun
L
L
Semmelweis University Medical School, First Institute of Biochemistry, Budapest, Hungary.
Garzó
T
T
Riba
P
P
Mandl
J
J
Kalapos
M P
MP
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011765:Pyruvaldehyde / Q000494:pharmacology*; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid; D014343:Trypan Blue / Q000378:metabolism
D000596:Amino Acids; D003470:Culture Media; D011773:Pyruvates; D011765:Pyruvaldehyde; D019289:Pyruvic Acid; D005978:Glutathione; D014343:Trypan Blue; D005947:Glucose