Effects of sterigmatocystin and T-2 toxin on the induction of unscheduled DNA synthesis in primary cultures of human gastric epithelial cells.
Primary cultures of human gastric epithelial cells were tested for induction of unscheduled DNA synthesis (UDS) by sterigmatocystin (ST) and T-2 toxin. Autoradiographic results indicated that ST (10(-6)-10(-4)M) induced UDS in the presence of S9 activation system. The repair rates were 24-91% (net grains > or = 3) and 2-71% (net grains > or = 5). T-2 toxin did not induce UDS in this study.
Ji
X
X
Laboratory of Mycotoxicology, Beijing Institute for Cancer Research, China.
Ke
Y
Y
Ning
T
T
Liang
Y
Y
Wang
D
D
Shi
G
G
D000818:Animals; D001345:Autoradiography; D001711:Biotransformation; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013241:Sterigmatocystin / Q000493:pharmacokinetics / Q000633:toxicity*; D013605:T-2 Toxin / Q000493:pharmacokinetics / Q000633:toxicity*
D013241:Sterigmatocystin; D004247:DNA; D013605:T-2 Toxin