Monoclonal antibody raised against apoprotein of C-1027: effect on biochemical and biological activities of the holoantibiotic.
A monoclonal antibody raised against C-1027 apoprotein recognized not only the apoprotein, but also the holoantibiotic (Antibiotic C-1027) almost equally. Among the biochemical and biological activities of the holoantibiotic, the antibody inhibited the aminopeptidase activity and the cytotoxicity to Ehrlich carcinoma cells in cultures, but not the DNA-cleaving activity in vitro. The immunohistogram, using this antibody, of Ehrlich carcinoma cells that had been exposed to the holoantibiotic suggested penetration of the holoantibiotic into target cells.
S-Tsuchiya
K
K
Showa College of Pharmaceutical Sciences, Tokyo, Japan.
Arita
M
M
Hori
M
M
Otani
T
T
D000617:Aminoglycosides; D000818:Animals; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D000918:Antibody Specificity; D001059:Apoproteins / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy; D004347:Drug Interactions; D053281:Enediynes; D007074:Immunoglobulin G / Q000494:pharmacology; D010455:Peptides
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D000911:Antibodies, Monoclonal; D001059:Apoproteins; D053281:Enediynes; D007074:Immunoglobulin G; D010455:Peptides; C058024:C 1027