title: Iron-responsive genetic regulation in Campylobacter jejuni: cloning and characterization of a fur homolog.
abstract: The Fur protein of Escherichia coli represses transcription from Fur-responsive genes in an iron-dependent manner. We have demonstrated a Fur-like iron-responsive genetic regulatory activity operating in Campylobacter jejuni by using a chloramphenicol acetyl transferase reporter gene separated from its promoter by a synthetic Fur-responsive operator. A fur-like gene has been cloned from C. jejuni by partial functional complementation of an E. coli fur mutation. Sequence analysis has shown that, at the amino acid level, the C. jejuni Fur protein is 35% identical with its E. coli counterpart.
authors.affiliation: Department of Genetics, University of Leicester, England.
authors.forename: K G
authors.initials: KG
authors.lastname: Wooldridge
authors.forename: P H
authors.initials: PH
authors.lastname: Williams
authors.forename: J M
authors.initials: JM
authors.lastname: Ketley
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D016123:Campylobacter jejuni / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D005816:Genetic Complementation Test; D007501:Iron / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; D007501:Iron; D015500:Chloramphenicol O-Acetyltransferase