Antibodies to a short synthetic peptide of the PEST region cross-react with mammalian tyrosine aminotransferase.
The immunoreactivity of the PEST region of mammalian tyrosine aminotransferase (TATase) was studied with a specific probe. An antipeptide serum was prepared using a synthetic peptide 385EFENDVEFTER395 (ER) corresponding to the main part of this important region of the liver enzyme. The antibodies were purified by affinity chromatography and analyzed by enzyme linked immunosorbent assay. Their use in immunoblotting experiments allowed the easy detection of the complete TATase molecule. The very high homology in rat and the human enzyme having the same PEST region, make these antibodies of interest for further studies of the human TATase.
Dietrich
J B
JB
IBMC du CNRS, Laboratoire de Biochimie, Strasbourg, France.
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D006801:Humans; D015151:Immunoblotting; D007120:Immunochemistry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014444:Tyrosine Transaminase / Q000737:chemistry / Q000276:immunology*
D009842:Oligopeptides; D014444:Tyrosine Transaminase