Complete sequence of the Bacteroides fragilis YCH46 neuraminidase-encoding gene.
The nucleotide sequence of the Bacteroides fragilis neuraminidase-encoding gene (nanH) and its flanking regions was determined. The B. fragilis nanH open reading frame (ORF) of 1632 nucleotides encoded a neuroaminidase of 544 amino acid residues with a calculated molecular mass of 59.51 kDa. The amino acid sequence at the N-terminus of the deduced protein exhibited the common features of procaryotic signal sequences. The Asp box sequence, Ser-X-Asp-X-Gly-X-Thr-Trp, which is conserved among bacterial neuraminidases, was found at five positions in the deduced amino acid sequence. A single transcription start point was identified 88 nucleotides upstream of the nanH ORF. Northern blot analysis using an internal nanH probe revealed a single mRNA species with a length of 1.8 kb that could encode just the neuraminidase.
Akimoto
S
S
Department of Bacteriology, School of Medicine, University of Tokushima, Japan.
Ono
T
T
Tsutsui
H
H
Kinouchi
T
T
Kataoka
K
K
Ohnishi
Y
Y
D000595:Amino Acid Sequence; D001441:Bacteroides fragilis / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D016384:Consensus Sequence; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D009439:Neuraminidase / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D009439:Neuraminidase