Toxicological analysis of whole blood samples by means of Bond-Elut Certify columns and gas chromatography with nitrogen-phosphorus detection.
The application of Bond-Elut Certify solid-phase extraction columns to the systematic toxicological analysis of whole blood was evaluated. The reproducibility of the extraction was tested with thirteen drugs varying in physico-chemical properties. Analysis was performed with capillary gas chromatography with nitrogen-selective detection. The recoveries were reproducible, as long as other limiting factors, e.g., chromatographic behaviour or volatility, do not play a significant role. The effect of limiting chromatographic behaviour was studied in more detail with the more sensitive mass spectrometry with selected-ion monitoring after converting the extracted morphine into its ditrimethylsilyl derivative.
Zweipfenning
P G
PG
Forensic Science Laboratory, Rijswijk, Netherlands.
Wilderink
A H
AH
Horsthuis
P
P
Franke
J P
JP
de Zeeuw
R A
RA
D001774:Blood Chemical Analysis; D002849:Chromatography, Gas / Q000379:methods*; D064420:Drug-Related Side Effects and Adverse Reactions; D006801:Humans; D009020:Morphine / Q000097:blood; D009584:Nitrogen; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010758:Phosphorus; D015203:Reproducibility of Results
D004364:Pharmaceutical Preparations; D010758:Phosphorus; D009020:Morphine; D009584:Nitrogen