Quality assessment for immunophenotyping of leukaemias and lymphomas: the Dutch experience. The SIHON Study Group.
Sending material at regular intervals to laboratories that perform immunophenotyping of leukaemias and lymphomas showed a large variety in the percentage of positive cells between the different laboratories but an agreement in diagnosis of more than 90%. Each laboratory used his own methods but antibody panels and guide-lines for interpretation were standardised. Studies are in progress to answer the question if standardisation in flow cytometry will result in more comparable results.
van't Veer
M B
MB
Department of Haematology, Rotterdam, The Netherlands.
Gratama
J W
JW
D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping / Q000592:standards*; D007938:Leukemia / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*