pH dependence of the absorption spectra and photochemical transformations of the archaerhodopsins.
Two strains of archaebacteria have been found to contain light-driven proton pumping pigments analogous to bacteriorhodopsin (bR) in Halobacterium salinarium. These proteins are called archaerhodopsin-1 (aR-1) and archaerhodopsin-2 (aR-2). Their high degree of sequence identity with bR within the putative proton channel enables us to draw some conclusions about the roles of regions where differences in amino acids exist, and in particular the surface residues, on the structure and function of retinal-based proton pumps. We have characterized the spectral and photochemical properties of these two proteins and compared them to the corresponding properties of bR. While there are some differences in absorbance maxima and kinetics of the photocycle, most of the properties of aR-1 and aR-2 are similar to those of bR. The most striking differences of these proteins with bR are the lack of an alkaline-induced red-shifted absorption species and a dramatic (apparent) decrease in the light-induced transient proton release. In membrane sheet suspensions of aR-1 at 0.15 M KCl, the order of proton release and uptake appears opposite that of bR, in which proton release precedes uptake. The nature of this behavior appears to be due to differences in the amino acid sequence at the surfaces of the proteins. In particular, the residue corresponding to the lysine at position 129 of the extracellular loop region of bR is a histidine in aR-1 and could regulate the efficient release of protons into solution in bR.
Lukashev
E P
EP
Biophysics Division, University of Illinois, Urbana 61801.
Govindjee
R
R
Kono
M
M
Ebrey
T G
TG
Sugiyama
Y
Y
Mukohata
Y
Y
D019843:Archaeal Proteins; D001426:Bacterial Proteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010777:Photochemistry; D017494:Proton Pumps / Q000737:chemistry*; D012168:Retinal Pigments / Q000737:chemistry; D013053:Spectrophotometry
D019843:Archaeal Proteins; D001426:Bacterial Proteins; D017494:Proton Pumps; D012168:Retinal Pigments; C055476:archaerhodopsin protein, Archaea