Identification of a conditionally essential heat shock protein in Escherichia coli.
Protein D48.5 was recognized as a heat-inducible protein of Escherichia coli during the screening of a group of random, temperature-inducible Mud-Lac fusion mutants. Physiological and genetic analysis demonstrated that (i) the structural gene for this protein, designated htpI, is a member of the sigma 32-dependent heat shock regulon, (ii) at 37 degrees C the synthesis of protein D48.5 is nearly constitutive, increasing slightly with growth rate in media of different composition, and (iii) this protein is essential for growth at high temperature.
Peruski
L F
LF
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
Neidhardt
F C
FC
D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000737:chemistry*; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000494:pharmacology / Q000502:physiology; D006358:Hot Temperature; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor / Q000494:pharmacology / Q000502:physiology; D014157:Transcription Factors; D001616:beta-Galactosidase / Q000235:genetics
D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32; D001616:beta-Galactosidase