Antibodies to the serine rich Entamoeba histolytica protein (SREHP) prevent amoebic liver abscess in severe combined immunodeficient (SCID) mice.
Amoebic liver abscess caused by Entamoeba histolytica is a major cause of morbidity and mortality worldwide. We used mice with severe combined immunodeficiency (SCID mice) to study the role of antibody in protection from amoebic liver abscess, and to identify protective antigens of E. histolytica. Antisera to recombinant versions of two major surface antigens of E. histolytica, the serine rich E. histolytica protein (SREHP) and the 170 kDa adhesin were used in this study. We found that 100% of SCID mice passively immunized with antiserum to the recombinant SREHP molecule were protected from developing amoebic liver abscess after intrahepatic challenge with virulent E. histolytica trophozoites. In contrast, preimmune serum, antiserum to a portion of the 170 kDa adhesin, and antiserum to the trpE fusion partner of SREHP did not protect SCID mice from amoebic liver abscess. Our study demonstrates that antibodies to a recombinant version of the amoebic SREHP molecule can protect against amoebic liver abscess, and suggest the recombinant SREHP molecule should be considered as a possible vaccine candidate to prevent amoebic liver abscess.
Zhang
T
T
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Cieslak
P R
PR
Foster
L
L
Kunz-Jenkins
C
C
Stanley
S L
SL
D000818:Animals; D000913:Antibodies, Protozoan / Q000627:therapeutic use*; D000953:Antigens, Protozoan / Q000276:immunology; D016466:CHO Cells; D002448:Cell Adhesion; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000276:immunology*; D007116:Immunization, Passive; D008101:Liver Abscess, Amebic / Q000517:prevention & control*; D051379:Mice; D016513:Mice, SCID; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012694:Serine / Q000276:immunology; D016511:Severe Combined Immunodeficiency / Q000150:complications*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011993:Recombinant Fusion Proteins; D012694:Serine