In vitro changes in platelet function and metabolism following increasing doses of ultraviolet-B irradiation.
Ultraviolet-B (UV-B) irradiation of platelet concentrates (PCs) may prevent the development of posttransfusion HLA alloimmunization. This study evaluated the effect of increasing doses of UV-B radiation on stored PCs. Pooled PCs were irradiated at UV-B doses of 600, 2400 or 10,000 mJ per cm2 and stored up to 96 hours under standard blood bank conditions. Compared to nonirradiated room-temperature and 37 degrees C controls, the irradiated units showed no significant changes in platelet count, white cell count, discharge of lactate dehydrogenase, release of beta-thromboglobulin, metabolism of ATP, ADP, ammonia, glutamine, glutamate, hypoxanthine, pCO2, or pO2 at any time of storage following any of the three UV-B doses. However, after a dose of 10,000 mJ per cm2, there were significant decreases in in vitro assays of platelet function-specifically, osmotic recovery and morphology score. Some metabolic systems were also affected by the 10,000 mJ per cm2 radiation dose, as shown by a decline in pH and bicarbonate and an increase in glucose consumption and lactate production (p < 0.05). The changes in these latter assays appeared only after 96 hours of postirradiation storage. Such changes were not seen in either the room-temperature or 37 degrees C control groups. Thus, heat generated during irradiation, per se, did not appear responsible for the observed in vitro changes in platelet function and metabolism. On the basis of the assays analyzed, it is concluded that UV-B irradiation of PCs at doses up to 10,000 mJ per cm2 does not induce significant metabolic or functional derangements following short-term storage (24-48 hours).(ABSTRACT TRUNCATED AT 250 WORDS)
Johnson
R B
RB
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut.
Napychank
P A
PA
Murphy
S
S
Snyder
E L
EL
D000244:Adenosine Diphosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001639:Bicarbonates / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000097:blood; D018698:Glutamic Acid; D005973:Glutamine / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000097:blood; D007773:Lactates / Q000378:metabolism; D007958:Leukocyte Count / Q000528:radiation effects; D009995:Osmosis / Q000528:radiation effects; D010976:Platelet Count / Q000528:radiation effects; D013696:Temperature; D014466:Ultraviolet Rays
D001639:Bicarbonates; D005971:Glutamates; D007042:Hypoxanthines; D007773:Lactates; D005973:Glutamine; D019271:Hypoxanthine; D018698:Glutamic Acid; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D005947:Glucose