Cadmium resistance of achlorophyllous Euglena gracilis cells: constitutive overexpression of two heat-shock proteins.
The heat-shock response of Euglena gracilis was studied by cell labeling at both the normal growth temperature (23 degrees C) and an elevated temperature (35 degrees C). Analysis of the labeled proteins by two-dimensional polyacrylamide gel electrophoresis indicated that the rate of synthesis of two polypeptides p55 (55 kDa) and p40 (40 kDa) increased in cells labeled at the highest temperature studied. These polypeptides are also overexpressed in Cadmium-resistant Euglena gracilis cells labeled at the normal growth temperature (23 degrees C). On the basis of these results, p55 and p40 appear to be heat-shock proteins involved in some steps of the acquired Cd-resistance process in Euglena gracilis cells.
Barque
J P
JP
Laboratoire Métabolisme Cellulaire et Xénobiotiques, Faculté de Pharmacie, Châtenay-Malabry, France.
Chacun
H
H
Marouby
S
S
Bonaly
J
J
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D004351:Drug Resistance / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005056:Euglena gracilis / Q000187:drug effects* / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D008970:Molecular Weight
D006360:Heat-Shock Proteins; D002104:Cadmium