title: Inhibition of canine NK activity by anti-CD18 monoclonal antibody, UV irradiation and cyclosporine.
abstract: Previous studies from our laboratory have shown that large granular lymphocytes (LGL) mediate natural killer cell (NK) activity in dogs and that host LGL are associated with graft failure in a canine model of histoincompatible marrow transplantation. We now report studies on the effects of anti-CD18 monoclonal antibody (MAB) 60.3, ultraviolet (UV) irradiation and cyclosporine (CSP) on in vitro canine NK function. Treatment with the murine anti-CD18 MAB 60.3 produced marked diminution in lytic function (mean 79% inhibition). UV irradiation at doses greater than 1.6 millijoules/cm2 completely eliminated NK function; this effect was not due to defective effector/target conjugate formation. CSP also inhibited NK function, although in vitro lytic activity was not completely blocked (mean of 44% inhibition) at concentrations which correspond to those achieved in vivo (400 ng/mL). These observations form a basis for future investigations aimed at preventing graft failure in the canine model of histoincompatible marrow transplantation by blocking NK function in vivo.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA.
authors.forename: T P
authors.initials: TP
authors.lastname: Loughran
authors.forename: H J
authors.initials: HJ
authors.lastname: Deeg
authors.forename: R
authors.initials: R
authors.lastname: Storb
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D015703:Antigens, CD / Q000276:immunology*; D018821:CD18 Antigens; D016572:Cyclosporine / Q000494:pharmacology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000502:physiology* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018821:CD18 Antigens; D016572:Cyclosporine