Characterization of the proline-rich region of mouse MAPKAP kinase 2: influence on catalytic properties and binding to the c-abl SH3 domain in vitro.
The primary structure of mouse MAP kinase-activated protein (MAPKAP) kinase 2 contains a proline-rich N-terminal region which might function as a src-homology 3 (SH3) domain-binding motif in vivo. To demonstrate the ability of this region to bind SH3 domains, we analyzed the interaction of the SH3 domain of the protein tyrosine kinase c-abl with MAPKAP kinase 2. It is demonstrated, that the proline-rich region specifically binds c-abl-SH3 domain in vitro. Furthermore, it is shown, that deletion of this proline-rich region does not significantly influence the substrate binding properties of the enzyme when analyzed with the substrate small heat shock protein Hsp25. The data suggest that the proline-rich region of MAPKAP kinase 2 could interact with proteins containing SH3-domains also in vivo regulating its cellular localization and/or modulating its enzymatic properties.
Plath
K
K
Max-Delbrück-Centrum for Molecular Medicine, Berlin, Germany.
Engel
K
K
Schwedersky
G
G
Gaestel
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002384:Catalysis; D018076:DNA, Complementary / Q000737:chemistry; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D011392:Proline; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000378:metabolism*; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D000099190:MAP-Kinase-Activated Kinase 2
D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D011392:Proline; D017346:Protein Serine-Threonine Kinases; D016315:Proto-Oncogene Proteins c-abl; D013438:Sulfhydryl Compounds; D000099190:MAP-Kinase-Activated Kinase 2