title: Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates melatonin synthesis from rat pineal gland.
abstract: The effect of pituitary adenylate cyclase polypeptide (PACAP) on rat pineal was examined. PACAP stimulated melatonin release from cultured dissociated pinealocytes with a 10(4) higher potency than isoproterenol (EC50 were 30 pM and 250 nM, respectively). The 10(-9) M PACAP stimulation was not inhibited by 5 x 10(-6) M VIP antagonist whereas that of 10(-9) M VIP was reduced by 54%. Kinetic analysis of melatonin release indicated that PACAP acts postsynaptically via receptor activation.
authors.affiliation: Neurobiologie des Fonctions Rythmiques et Saisonnières, URA-CNRS 1332, Université Louis Pasteur, Strasbourg, France.
authors.forename: V
authors.initials: V
authors.lastname: Simonneaux
authors.forename: A
authors.initials: A
authors.lastname: Ouichou
authors.forename: P
authors.initials: P
authors.lastname: Pévet
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008550:Melatonin / Q000096:biosynthesis*; D009479:Neuropeptides / Q000494:pharmacology*; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D010477:Perfusion; D010870:Pineal Gland / Q000187:drug effects / Q000378:metabolism*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats; D017208:Rats, Wistar; D011964:Receptors, Gastrointestinal Hormone / Q000037:antagonists & inhibitors; D018005:Receptors, Vasoactive Intestinal Peptide; D013268:Stimulation, Chemical; D014660:Vasoactive Intestinal Peptide / Q000037:antagonists & inhibitors / Q000494:pharmacology
chemical_list: C495403:Adcyap1 protein, rat; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D008550:Melatonin; D007545:Isoproterenol