title: Purification and molecular cloning of human apolipoprotein F.
abstract: In our effort to study proteins that are involved in high density lipoprotein metabolism, we have identified apolipoprotein F and isolated a full length cDNA clone. Apolipoprotein F, with an apparent molecular mass of 29 kilodaltons, was purified from human high density lipoproteins using a modified two dimensional electrophoresis procedure. The cDNA, with a size of 1735 base pairs, was cloned from a Hep G2 cDNA library. The cDNA encodes apolipoprotein F, which is composed of 162 amino acids, and predicts that apolipoprotein F is a proteolytic product of a larger protein. Northern blot analysis indicates that apolipoprotein F mRNA is detected only in liver for the tissues examined. The gene was mapped to human chromosome number 12 using a human/rodent somatic cell hybrid mapping panel.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98103.
authors.forename: J R
authors.initials: JR
authors.lastname: Day
authors.forename: J J
authors.initials: JJ
authors.lastname: Albers
authors.forename: T L
authors.initials: TL
authors.lastname: Gilbert
authors.forename: T E
authors.initials: TE
authors.lastname: Whitmore
authors.forename: W J
authors.initials: WJ
authors.lastname: McConathy
authors.forename: G
authors.initials: G
authors.lastname: Wolfbauer
mesh_terms: D000595:Amino Acid Sequence; D001053:Apolipoproteins / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D006031:Glycosylation; D006801:Humans; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D014357:Trypsin / Q000378:metabolism
chemical_list: D001053:Apolipoproteins; D018076:DNA, Complementary; D010446:Peptide Fragments; D012333:RNA, Messenger; C036321:apolipoprotein F; D014357:Trypsin