title: Effect of growth hormone on wound healing in protein-malnourished rats treated with corticosteroids.
abstract: This study was designed to evaluate the efficacy of human growth hormone (GH) in improving the tensile strength of wounds weakened by chronic protein malnourishment and corticosteroid (CS) administration. Eighty-six female Sprague-Dawley rats, weighing 80 to 100 g, were divided into five groups. Group 1 (control) received 23.4% protein chow for 8 weeks before surgery. Groups 2, 3, 4, and 5 received nonprotein chow on alternate days for the same duration. Groups 3 and 5 received prednisolone (2 mg/kg/d intramuscularly) for 3 weeks preoperatively and for 5 days postoperatively. Groups 4 and 5 were given GH (somatotropin, 1 IU/d) for 5 days postoperatively. All the animals underwent a precise 4-cm midline celiotomy. Wound testing was performed on the sixth postoperative day, after removal of the sutures. The bursting strength (BS, mean +/- SD) for group 1 was 145 +/- 16 mm Hg. The BS for groups 4 (137 +/- 13 mm Hg) and 5 (134 +/- 7 mm Hg) were significantly stronger than those for groups 2 (115 +/- 15 mm Hg) and 3 (91 +/- 16 mm Hg). The authors conclude that postoperative systemic GH restored the wound BS in protein-malnourished animals treated with CS, to the level of the normally nourished controls.
authors.affiliation: Second Department of Surgery, Yamanashi Medical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takano
authors.forename: N
authors.initials: N
authors.lastname: Mouri
authors.forename: H
authors.initials: H
authors.lastname: Sakurai
authors.forename: S
authors.initials: S
authors.lastname: Muto
authors.forename: K
authors.initials: K
authors.lastname: Koshizuka
authors.forename: Y
authors.initials: Y
authors.lastname: Tada
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000596:Amino Acids / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D005260:Female; D005353:Fibronectins / Q000032:analysis; D013006:Growth Hormone / Q000494:pharmacology*; D011239:Prednisolone / Q000494:pharmacology*; D011502:Protein-Energy Malnutrition / Q000097:blood / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000596:Amino Acids; D001798:Blood Proteins; D005353:Fibronectins; D013006:Growth Hormone; D011239:Prednisolone; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase