Intraventricular application of BDNF and NT-3 failed to protect nucleus basalis magnocellularis cholinergic neurones.
Quantitative analysis of ChAT immunoreactive neurones was used to evaluate the protective potential of BDNF or NT-3 against retrograde changes in nucleus basalis magnocellularis (nbm) cholinergic neurones after unilateral partial devascularization of the rat neocortex. A daily intracerebroventricular dose of 12 micrograms, proven to be effective for NGF and aFGF in the same experimental paradigm, was administered by minipump infusion for a 1-week period. Thirty days after lesioning, neuronal shrinkage and loss of neuritic processes were not prevented by treatment. The results indicate that intracerebroventricularly delivered BDNF and NT-3 are not as effective as NGF and aFGF in protection of nbm cholinergic neurones against lesion-induced changes in adult rat brain.
Skup
M H
MH
Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
Figueiredo
B C
BC
Cuello
A C
AC
D000818:Animals; D017776:Autonomic Pathways / Q000166:cytology / Q000187:drug effects; D001479:Basal Ganglia / Q000166:cytology* / Q000187:drug effects; D019208:Brain-Derived Neurotrophic Factor; D002540:Cerebral Cortex / Q000098:blood supply; D002560:Cerebrovascular Circulation / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D008297:Male; D009414:Nerve Growth Factors / Q000008:administration & dosage / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D020933:Neurotrophin 3; D010275:Parasympathetic Nervous System / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3; D002795:Choline O-Acetyltransferase