Effects of oxygen free radical scavengers on uranium-induced acute renal failure in rats.
Study was made to determine whether oxygen free radicals mediate uranium-induced acute renal failure (ARF). Superoxide dismutase (SOD), a superoxide anion scavenger, did not prevent uranium acetate (UA) (5 mg/kg, i.v.)-induced renal injury 48 h after injection. In contrast, dimethylthiourea (DMTU), a hydroxyl radical scavenger, significantly attenuated UA-induced rise in serum creatinine concentration (1.11 +/- 0.05 (DMTU) vs. 1.40 +/- 0.06 mg/dl (control), p < .05), and tubular necrosis. Dimethyl sulfoxide (DMSO), a hydroxyl radical scavenger, decreased UA-induced tubular damage. UA injection caused no increase in renal cortical malondialdehyde (MDA) content. DMTU and DMSO did not modify intrarenal MDA content. UA administration brought about significant increase in plasma renin activity but not in renal cortical renin content. Treatment with DMTU and DMSO had no effect on plasma renin activity or intrarenal renin content. It follows from these findings that DMTU and DMSO may attenuate UA-induced renal injury. Such a protective effect would not be mediated through modulation of lipid peroxidation or renin activity.
Kato
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A
First Department of Medicine, Hamamatsu University School of Medicine, Japan.
Hishida
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A
Nakajima
T
T
D058186:Acute Kidney Injury / Q000139:chemically induced / Q000503:physiopathology* / Q000652:urine; D000704:Analysis of Variance; D000818:Animals; D001806:Blood Urea Nitrogen; D001835:Body Weight / Q000187:drug effects; D003404:Creatinine / Q000097:blood; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D016166:Free Radical Scavengers; D006400:Hematocrit; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology; D007672:Kidney Cortex / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000378:metabolism; D013482:Superoxide Dismutase / Q000494:pharmacology*; D013599:Systole / Q000187:drug effects; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology; D014501:Uranium / Q000633:toxicity*
D016166:Free Radical Scavengers; D014501:Uranium; D008315:Malondialdehyde; C038983:1,3-dimethylthiourea; D003404:Creatinine; D013482:Superoxide Dismutase; D012083:Renin; D013890:Thiourea; D004121:Dimethyl Sulfoxide