Effect of centrifuging shell vials at 3,500 x g on detection of viruses in clinical specimens.
An increase in shell vial centrifugation force to 3,500 x g and a concomitant reduction in spin time to 15 min did not decrease the sensitivity of detecting viruses in clinical specimens compared with the accepted practice of using 700 x g for 40 min. No damage to the cell monolayer (ML) at the higher g force was observed. Toxicity to the ML is decreased with the shorter spin, probably because of reduced time of contact between the specimen and the ML.
Engler
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Clinical Pathology Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-0001.
Selepak
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D002498:Centrifugation; D005069:Evaluation Studies as Topic; D006801:Humans; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013997:Time Factors; D014773:Virology / Q000295:instrumentation / Q000379:methods*; D014777:Virus Diseases / Q000382:microbiology*; D014780:Viruses / Q000302:isolation & purification*