Expression of intercellular adhesion molecule-1 on transitional cell cancer. Possible significance in immunity against tumor cells.
Immunohistochemical examination demonstrated expression of intercellular adhesion molecule-1 (ICAM-1) on 17 of 44 transitional cell cancers (TCCs) but not on normal transitional cells. ICAM-1 was frequently expressed in higher stage tumors, especially in those with abundant immune cells scattered within tumor. Analysis of infiltrating immune cells showed that they were composed mainly of T lymphocytes and a smaller number of macrophages bearing the lymphocyte function-associated antigen-1 (LFA-1). Expression of ICAM-1 on transitional cell cancer cell lines was augmented by in vitro treatment with interferon-gamma, tumor necrosis factor-alpha, and interleukin-1 beta. Furthermore, Northern blot analysis revealed higher quantities of a 3.3-kb RNA in T24 cells exposed to interferon-gamma or tumor necrosis factor-alpha. These results suggest that the expression of ICAM-1 on transitional cell cancers might be modified by cytokines produced by infiltrating immune cells, which might facilitate immune responses against cancer cells.
Tomita
Y
Y
Department of Urology, Niigata University School of Medicine, Japan.
Watanabe
H
H
Kobayashi
H
H
Nishiyama
T
T
Tsuji
S
S
Imai
K
K
Abo
T
T
Fujiwara
M
M
Sato
S
S
D000368:Aged; D000369:Aged, 80 and over; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology; D002295:Carcinoma, Transitional Cell / Q000737:chemistry*; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000494:pharmacology; D016246:Lymphocytes, Tumor-Infiltrating / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000166:cytology; D014407:Tumor Cells, Cultured / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014516:Ureteral Neoplasms / Q000737:chemistry; D001749:Urinary Bladder Neoplasms / Q000737:chemistry
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D016207:Cytokines; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma