Features of the cellodextrinase gene from Fibrobacter succinogenes S85.
The nucleotide sequence of a 2.3-kb DNA fragment containing a cellodextrinase gene (cedA) from the ruminal anaerobe Fibrobacter succinogenes S85 was determined. Activity was expressed from this fragment when it was cloned in both orientations in pBluescript KS+ and SK-, indicating a functional F. succinogenes promoter in Escherichia coli. Promoter sequences (TTGAACA and AATAA) were identified upstream of the ATG initiation codon preceded by a putative ribosome binding site. The cedA open reading frame of 1071 base pairs encoded a protein of 357 amino acid residues with a calculated molecular mass of 41.9 kDa, similar to the 40-kDa size of the native protein as determined by gel filtration chromatography. CedA is proposed to belong to family 5 (family A) of the glycosyl hydrolases. The primary structure of the cellodextrinase showed over 40% similarity with endoglucanase 3 from F. succinogenes S85. Short regions of similarity were also demonstrated with endoglucanase C from Clostridium thermocellum, CelA from Ruminococcus flavefaciens, and two exoglucanases from yeast.
Iyo
A H
AH
Department of Microbiology, University of Guelph, ON, Canada.
Forsberg
C W
CW
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli; D005798:Genes, Bacterial; D006089:Gram-Negative Anaerobic Bacteria / Q000235:genetics*; D008969:Molecular Sequence Data
D001426:Bacterial Proteins; C066565:ced1 protein, Butyrivibrio fibrisolvens; D002480:Cellulase