title: Comparison of cytogenetic and restriction fragment length polymorphism analyses for the detection of loss of chromosome material in clonal hemopoietic disorders.
abstract: Loss of chromosome material, as manifested by monosomy or partial deletion, is commonly found in neoplastic cells. We have undertaken a systematic comparison of standard cytogenetic analysis and molecular analysis for the detection of such loss, using as a model loss of chromosome 7 in 72 patients with a clonal myeloid malignancy. A large number of probes was used to screen three regions of chromosome 7 for loss by restriction fragment length polymorphism (RFLP) analysis. There were nine cases in which loss of chromosome 7 was detected by both techniques, but seven in which loss was detected by only one of the methods, demonstrating the complementary nature of these two techniques.
authors.affiliation: Leukaemia Research Fund Molecular and Cytogenetic Haematology Unit, Oxford, United Kingdom.
authors.forename: G M
authors.initials: GM
authors.lastname: Abrahamson
authors.forename: K
authors.initials: K
authors.lastname: Rack
authors.forename: D G
authors.initials: DG
authors.lastname: Oscier
authors.forename: M
authors.initials: M
authors.lastname: Fitchett
authors.forename: V J
authors.initials: VJ
authors.lastname: Buckle
authors.forename: J S
authors.initials: JS
authors.lastname: Wainscoat
mesh_terms: D000368:Aged; D002872:Chromosome Deletion; D002897:Chromosomes, Human, Pair 7; D003582:Cytogenetics / Q000379:methods*; D004247:DNA / Q000235:genetics; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004247:DNA