Cloning of genomic loci and chromosomal localization of the human PCTAIRE-1 and -3 protein kinase genes.
Recent studies on the molecular mechanisms controlling the mammalian cell cycle have disclosed a large family of cdc2-related serine/threonine kinases. Among this gene family, the PCTAIRE protein kinases comprise a distinct subfamily of unknown cellular function. To analyze the genomic structure and chromosomal location of the PCTAIRE-1 and -3 genes, we isolated human cosmid clones for each gene by screening a human genomic library with murine PCTAIRE cDNA probes. Overlapping clones encompassing approximately 60 kb of genomic DNA were obtained for both PCTAIRE-1 and -3. These clones were confirmed to encode authentic PCTAIRE genes by the detection of exon-intron structures and the coincidence of the nucleotide sequence of exons to that of the published human cDNAs. Using these cosmid clones as probes for FISH analyses, the chromosomal loci for PCTAIRE-1 and PCTAIRE-3 were assigned to bands Xp11 and 1q31-q32, respectively.
Okuda
T
T
Department of Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
Valentine
V A
VA
Shapiro
D N
DN
Downing
J R
JR
D000818:Animals; D002453:Cell Cycle; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003360:Cosmids; D018844:Cyclin-Dependent Kinases; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D005810:Multigene Family; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D014960:X Chromosome
D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases; C089463:PCTAIRE-1 protein kinase; C089464:PCTAIRE-3 protein kinase