Isolation and characterization of early pregnancy factor.
Early pregnancy factor (EPF) was purified from the pooled sera of 210 pregnant women at 3-8 weeks of gestation. Sera from healthy nonpregnant women were used as control. The samples (G-II, G-III and G-IV) obtained from pregnant women had EPF activity but no HCG activity. Polyacrylamide gel electrophoresis showed that the major bands in pregnant G-III and G-IV were at similar positions in tube gels. The results of SDS-PAGE showed 3 bands in pregnant G-IV: 57.0 kD, 38.0 kD and 19.0 kD. The basic active form of EPF may be a small peptide of 19.0 kD. The isoelectric points of pregnant G-IV were 6.45 and 8.20.
Zuo
X
X
Anhui Medical University, Hefei.
Su
B
B
Wei
D
D
D018835:Chaperonin 10; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011247:Pregnancy / Q000097:blood*; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic
D018835:Chaperonin 10; D007166:Immunosuppressive Agents; D010455:Peptides; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; C025124:early pregnancy factor