title: Composition of 2,3-dihydroxy fatty acid-containing lipopolysaccharides from Legionella israelensis, Legionella maceachernii and Legionella micdadei.
abstract: Lipopolysaccharides (LPS) from Legionella israelensis, L. maceachernii and L. micdadei were analysed for chemical composition. The main sugar of the lipid A fractions was in each case 2,3-diamino-2,3-dideoxy-D-glucose. Lipid A of L. israelensis also contained a substantial amount of D-glucosamine. In each lipid A fraction a complex fatty acid pattern was detected. This comprised at least 19 different 3-hydroxy fatty acids (amide-linked), three 2,3-dihydroxy fatty acids (amide-linked), non-hydroxy fatty acids (ester-linked) as well as long-chain (omega-1)-oxo, (omega-1)-hydroxy and (1,omega)-dioic fatty acids (ester-linked). In addition, L. maceachernii and L. micdadei contained alpha-hydroxylated long-chain (omega-1)-oxo and (1,omega)-dioic fatty acids. The polysaccharide parts of L. maceachernii and L. micdadei LPS were similar and contained mainly L-rhamnose, L-fucose, D-mannose, D-glucose, L-fucosamine, D-glucosamine, 2-keto-3-deoxy-octonic acid (Kdo) as well as the rare octose yersiniose A. The corresponding composition of L. israelensis LPS was simpler and consisted mainly of L-rhamnose and 3-amino-3,6-dideoxy-D-mannose. LPS of L. israelensis and L. micdadei contained, in addition, 2-keto-octonic acid linked to Kdo. Phosphorylated sugar constituents were detected in all three LPS, whereas ethanolamine was found only in LPS from L. maceachernii. The SDS-PAGE band pattern of L. micdadei differed from the two others in a higher proportion of the low molecular mass constituents.
authors.affiliation: National Institute of Public Health, Oslo, Norway.
authors.forename: A
authors.initials: A
authors.lastname: Sonesson
authors.forename: E
authors.initials: E
authors.lastname: Jantzen
authors.forename: K
authors.initials: K
authors.lastname: Bryn
authors.forename: T
authors.initials: T
authors.lastname: Tangen
authors.forename: J
authors.initials: J
authors.lastname: Eng
authors.forename: U
authors.initials: U
authors.lastname: Zähringer
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods; D004187:Disaccharides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis*; D005944:Glucosamine / Q000031:analogs & derivatives / Q000032:analysis; D006868:Hydrolysis; D007875:Legionella / Q000737:chemistry* / Q000145:classification; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D013045:Species Specificity
chemical_list: D002241:Carbohydrates; D004187:Disaccharides; D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides; C040138:2,3-diamino-2,3-dideoxyglucose; D005944:Glucosamine