title: Requirement for B cell-derived immunoglobulin for Th activation by the type 2 antigen polyvinylpyrrolidone.
abstract: Th specific for the type 2 antigen polyvinylpyrrolidone (PVP) could not be activated in vivo or in vitro in mice rendered B cell deficient by treatment from birth with anti-IgM. The requirement for B cells was apparently not due to a requirement for B cells to function as antigen presenting cells for Th activation since T cells also were not activated when anti-IgM treated mice were reconstituted with B cells prior to priming with PVP. In addition, removal of B cells from spleens of adult of adult mice had no effect on the ability of PVP to activate Th in vitro. Potential non-specific effects of chronic anti-IgM treatment on T cell function are unlikely since T cells from anti-IgM treated mice provided help to B cells for antibody responses to horse red blood cells responded normally to T cell mitogens and had a CD4:CD8 ratio like that of untreated mice; PVP primed T cells from anti-IgM treated mice also did not actively suppress the helper activity of PVP primed T cells from normal mice. In addition, PVP-specific Th were able to be activated in mice given multiple injections of anti-IgM beginning 2 weeks after birth and in mice given MOPC 104E IgM together with anti-IgM; under these conditions B cell depletion by the anti-IgM did not occur. Thus activation of PVP-specific Th requires that B cells be present during the first few weeks after birth. Experiments in which mice were given injections of normal mice Ig together with the anti-IgM indicate that the function of B cells for Th activation in this system can be replaced by B cell-derived Ig. Thus PVP-specific Th could be activated in mice treated from birth with anti-IgM plus normal Ig despite the absence of significant numbers of B cells in the spleens of these mice.
authors.affiliation: Department of Medicine, University of Missouri, Columbia 65212.
authors.forename: H
authors.initials: H
authors.lastname: Braley-Mullen
authors.forename: G N
authors.initials: GN
authors.lastname: Milligan
authors.forename: A M
authors.initials: AM
authors.lastname: Van Buskirk
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006462:Hemolytic Plaque Technique; D007075:Immunoglobulin M / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology; D016219:Immunotherapy, Adoptive; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011205:Povidone; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D000941:Antigens; D007075:Immunoglobulin M; D007136:Immunoglobulins; D011205:Povidone