Effect of lysophosphatidic acid on the preimplantation development of mouse embryos.
The effect of lysophosphatidic acid (LPA) on the preimplantation development of mouse embryos was examined. The blastocyst rates from 2-cell and 4-cell stage embryos were significantly higher in the presence of LPA at the pronuclear stage. However, the blastocyst rate from 4-cell stage embryos was significantly lower when LPA was added to the medium at the 4-cell stage than when it was added at the pronuclear stage. Furthermore, pretreatment of pronuclear stage embryos with pertussis toxin suppressed LPA-induced embryo development, suggesting that it is probably mediated by a Gi-protein-linked receptor.
Kobayashi
T
T
Department of Obstetrics and Gynecology, School of Medicine, Tokushima, Japan.
Yamano
S
S
Murayama
S
S
Ishikawa
H
H
Tokumura
A
A
Aono
T
T
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D046508:Culture Techniques; D047108:Embryonic Development; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D008246:Lysophospholipids / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy
D008246:Lysophospholipids