Simple and efficient purification of C-reactive protein from canine serum.
Simple purification method for canine serum, C-reactive protein (CRP) was established. By combining an affinity chromatography on Sepharose 4B coupled with o-phosphorylethanolamine and a gel filtration on a column of Sephacryl S-300, about 9 mg of purified CRP from 50 ml of canine serum was obtained. The method is simple and efficient to purify canine CRP.
Onishi
T
T
Department of Veterinary Internal Medicine, College of Agriculture, University of Osaka Prefecture, Japan.
Shimizu
T
T
Kajikawa
T
T
D000818:Animals; D002097:C-Reactive Protein / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D004285:Dogs / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007202:Indicators and Reagents; D008970:Molecular Weight; D014425:Turpentine
D007202:Indicators and Reagents; D002097:C-Reactive Protein; D014425:Turpentine