Proteinergic profiles in cerebrospinal fluid from alcoholic subjects.
Minute amounts of cerebrospinal fluid samples from alcoholics were subjected to separation by HPLC-molecular sieving, combined with multispectral UV analysis of the acquired data. A significant difference in the protein/polypeptide pattern within the molecular weight range of 7-10 kDa has been observed between samples, taken directly after detoxification and 2 weeks later. Spectral analysis of the results suggests that the components are of peptidergic nature. On the other hand, albumin content did not differ significantly, suggesting that the blood-brain barrier was not affected. An enzyme marker, dynorphin converting enzyme, remained unchanged in both groups.
Silberring
J
J
Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden.
Brostedt
P
P
Neiman
J
J
Hellman
U
U
Liljequist
S
S
Terenius
L
L
D000328:Adult; D000368:Aged; D000418:Albumins / Q000134:cerebrospinal fluid; D000437:Alcoholism / Q000134:cerebrospinal fluid*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055316:Cystatin C; D015891:Cystatins / Q000134:cerebrospinal fluid; D006801:Humans; D007427:Intracranial Pressure; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet; D001613:beta 2-Microglobulin / Q000134:cerebrospinal fluid
D000418:Albumins; C525030:CST3 protein, human; D002556:Cerebrospinal Fluid Proteins; D055316:Cystatin C; D015891:Cystatins; D001613:beta 2-Microglobulin