Transcriptional regulation of the human E-cadherin gene in human prostate cancer cell lines: characterization of the human E-cadherin gene promoter.
Decreased expression of the Ca(2+)-dependent cell adhesion molecule E-cadherin is observed in several poorly differentiated carcinomas and is presumably associated with an invasive phenotype of these tumors. Evidence accumulated so far indicates that decreased transcription is a major mechanism leading to defective E-cadherin function. Therefore, we isolated and characterized the human E-cadherin gene promoter and studied the transcriptional regulation of this gene in two human prostate cancer cell lines, one expressing E-cadherin (PC-3), the other one not expressing E-cadherin (TSU-pr1). We show that the E-cadherin promoter is not active in the non-expressing cells and that this may be due to the binding of a repressor protein to the promoter.
Bussemakers
M J
MJ
Urology Research Laboratory, University Hospital Nijmegen, The Netherlands.
Giroldi
L A
LA
van Bokhoven
A
A
Schalken
J A
JA
D001483:Base Sequence; D015820:Cadherins / Q000235:genetics*; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion; D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011467:Prostate / Q000378:metabolism*; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D016385:TATA Box; D014158:Transcription, Genetic
D015820:Cadherins; D015719:Single-Strand Specific DNA and RNA Endonucleases; D002118:Calcium