Detection of a restriction fragment-length polymorphism in human endometrial carcinoma DNA with an alpha-tubulin cDNA probe.
We report a significant excess of heterozygotes at an alpha-tubulin locus in individuals with endometrial carcinoma. Although the heterozygous state was not specific for cancer, there was a 2.6-fold increase in heterozygote frequencies in endometrial tumors over those of normal human endometrial tissues. Among normal control tissues, the two alleles of 2.1 and 2.2 kb apparent size were found to be in a Hardy-Weinberg equilibrium. The results with tumor samples were significantly different from those expected for a Hardy-Weinberg distribution. The alleles identified using MspI digestion and a 2.5-kb alpha-tubulin cDNA probe have been previously reported by Gatti et al. (Nucleic Acids Res. 15, 8119, 1987).
Mittanck
D W
DW
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409.
Wigley
W C
WC
Li
X
X
Alecozay
A A
AA
Nakashima
R A
RA
D000483:Alleles; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis*; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D014404:Tubulin / Q000235:genetics*
D015342:DNA Probes; D004273:DNA, Neoplasm; D014404:Tubulin; D004247:DNA