Structure based prediction of protein folding intermediates.
The complete unfolding of a protein involves the disruption of non-covalent intramolecular interactions within the protein and the subsequent hydration of the backbone and amino acid side-chains. The magnitude of the thermodynamic parameters associated with this process is known accurately for a growing number of globular proteins for which high-resolution structures are also available. The existence of this database of structural and thermodynamic information has facilitated the development of statistical procedures aimed at quantifying the relationships existing between protein structure and the thermodynamic parameters of folding/unfolding. Under some conditions proteins do not unfold completely, giving rise to states (commonly known as molten globules) in which the molecule retains some secondary structure and remains in a compact configuration after denaturation. This phenomenon is reflected in the thermodynamics of the process. Depending on the nature of the residual structure that exists after denaturation, the observed enthalpy, entropy and heat capacity changes will deviate in a particular and predictable way from the values expected for complete unfolding. For several proteins, these deviations have been shown to exhibit similar characteristics, suggesting that their equilibrium folding intermediates exhibit some common structural features. Employing empirically derived structure-energetic relationships, it is possible to identify in the native structure of the protein those regions with the higher probability of being structured in equilibrium partly folded states. In this work, a thermodynamic search algorithm aimed at identifying the structural determinants of the molten globule state has been applied to six globular proteins; alpha-lactalbumin, barnase, IIIGlc, interleukin-1 beta, phage T4 lysozyme and phage 434 repressor. Remarkably, the structural features of the predicted equilibrium intermediates coincide to a large extent with the known structural features of the corresponding intermediates determined by NMR hydrogen-exchange experiments.
Xie
D
D
Department of Biology and the Biocalorimetry Center, Johns Hopkins University, Baltimore, MD 21218.
Freire
E
E
D001426:Bacterial Proteins; D007375:Interleukin-1 / Q000737:chemistry; D007768:Lactalbumin / Q000737:chemistry; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry; D011487:Protein Conformation; D017510:Protein Folding; D012097:Repressor Proteins / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D014764:Viral Proteins / Q000737:chemistry
C063367:434-repressor protein, Bacteriophage 434; D001426:Bacterial Proteins; D007375:Interleukin-1; D012097:Repressor Proteins; D014764:Viral Proteins; C057633:gene 25 protein, Enterobacteria phage T4; D007768:Lactalbumin; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease