Neuropeptide Y, tyrosine hydroxylase and vasoactive intestinal polypeptide-immunoreactive nerve fibers in the vertebral bodies, discs, dura mater, and spinal ligaments of the rat lumbar spine.
The occurrence of autonomic nerves in the lumbar spine of rats was investigated by immunohistochemical technique. Both peptidergic nerves, represented by immunoreactivity to neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), and noradrenergic nerves as reflected by tyrosine hydroxylase (TH) immunoreactivity, were identified. NPY- and TH-positive nerves were predominantly found in the blood vessels walls. They occurred in the bone and periosteum of the vertebral body, disc, dura mater, and in the spinal ligaments. They were particularly numerous along the growth plate and in the outer layers of the intervertebral discs. VIP-positive fibers were predominantly nonvascular. They occurred in all tissues analyzed, but were most abundant in the vertebral bone marrow and periosteum. The VIP-immunoreactive fibers in the outer fibrous layers of the disc and the spinal ligaments were occasionally observed in blood vessel walls. No immunoreactivity could be detected in the nucleus pulposus. In the dura mater, NPY-, TH- and VIP-positive fibers were found both in the ventral and dorsal portion. In view of the vasoconstrictive properties of both NPY and noradrenaline, it may be assumed that the abundance of NPY- and TH-immunoreactive nerves fibers in blood vessel walls reflects a vasoregulatory activity. The predominance of nonvascular VIP-positive fibers in the vertebral bone marrow and periosteum may represent an involvement in local bone physiology.
Ahmed
M
M
Department of Orthopaedics, Karolinska Institute, Stockholm, Sweden.
Bjurholm
A
A
Kreicbergs
A
A
Schultzberg
M
M
D000818:Animals; D007403:Intervertebral Disc / Q000294:innervation*; D008022:Ligaments / Q000294:innervation*; D008159:Lumbar Vertebrae / Q000098:blood supply / Q000294:innervation*; D008297:Male; D009412:Nerve Fibers / Q000737:chemistry*; D009478:Neuropeptide Y / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
D009478:Neuropeptide Y; D014660:Vasoactive Intestinal Peptide; D014446:Tyrosine 3-Monooxygenase