Isolation and characterization of two cDNAs encoding for compartment specific isoforms of O-acetylserine (thiol) lyase from Arabidopsis thaliana.
cDNAs encoding for two isoforms of O-acetylserine (thiol) lyase (OAS-TL), which catalyzes the synthesis of cysteine, have been isolated from Arabidopsis thaliana. Secondary structure together with expression patterns derived during photomorphogenesis indicate cellular localizations in the cytosol and plastids, thus allowing a direct comparison of compartment-specific forms within one species. The cytosolic OAS-TL complemented an E. coli auxotrophic mutant lacking cysteine synthesis. Both isoforms are represented by small gene families. They are expressed under all conditions investigated and were observed to increase in expression in plants grown with limited sulfate supply.
Hell
R
R
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Germany.
Bork
C
C
Bogdanova
N
N
Frolov
I
I
Hauschild
R
R
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D001483:Base Sequence; D002451:Cell Compartmentation; D003547:Cysteine Synthase / Q000096:biosynthesis / Q000235:genetics*; D003600:Cytosol / Q000201:enzymology*; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D017343:Genes, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics*; D008027:Light; D008969:Molecular Sequence Data; D018087:Plastids / Q000201:enzymology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D007527:Isoenzymes; D011994:Recombinant Proteins; D003547:Cysteine Synthase