Differential involvement of protein kinase C in transmitter release and response to excitatory amino acids in cultured cerebellar neurons.
Cerebellar granule cells cultured in the presence of a differentiating factor isolated from rabbit serum exhibit, at variance with those cultured in fetal calf serum, an almost complete resistance to excitatory aminoacid (EAA)-induced cytotoxicity. We investigated the behaviour of protein kinase C (PKC), strongly implicated in EAA cytotoxicity, in the two types of culture. Phorbol esters, used to monitor the enzyme, enhanced the depolarization-evoked release of D-[3H]aspartate, but less effectively in factor-conditioned cells. EAAs increased phorbol esters binding in both cultures, but the effect was briefly lasting in factor-conditioned cells. The different behaviour of PKC is postulated to be causally related to different response to EAA of the cultures.
Eboli
M L
ML
Institute of General Pathology, Catholic University, Rome, Italy.
Ciotti
M T
MT
Mercanti
D
D
Calissano
P
P
D000596:Amino Acids / Q000187:drug effects*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011493:Protein Kinase C / Q000187:drug effects / Q000502:physiology*; D051381:Rats
D000596:Amino Acids; D018377:Neurotransmitter Agents; D001224:Aspartic Acid; D011493:Protein Kinase C