Role of nitric oxide in hepatic injury following acute endotoxemia.
Hepatocytes from control and endotoxemic rats were cultured for 40 hours in 96-well dishes in medium containing 0-5 micrograms/ml of lipopolysaccharide in the presence or absence of 50 U/ml IFN gamma. Nitric oxide production was quantified by the accumulation of nitrite in the culture medium by the Greiss reaction. Hepatocyte proliferation and protein synthesis were measured by (3H)thymidine (TdR) and (3H)leucine (Leu) incorporation, respectively. Results are the mean +/- standard error of triplicate wells from four experiments.
Rodriguez del Valle
M
M
Rutgers University, Department of Pharmacology and Toxicology, Piscataway, New Jersey 08855-0789.
Hwang
S M
SM
Heck
D E
DE
Laskin
J D
JD
Laskin
D L
DL
D000208:Acute Disease; D000818:Animals; D002455:Cell Division; D004731:Endotoxins / Q000633:toxicity*; D004926:Escherichia coli / Q000472:pathogenicity*; D005260:Female; D008070:Lipopolysaccharides / Q000633:toxicity*; D008107:Liver Diseases / Q000209:etiology* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004731:Endotoxins; D008070:Lipopolysaccharides; D009569:Nitric Oxide