title: Visualization of an efferent projection route of the hypothalamic rat arcuate nucleus through the stria terminalis after labeling with carbocyanine dye (DiI) or proopiomelanocortin-immunohistochemistry.
abstract: The hypothalamic arcuate nucleus is known to be reciprocally connected to various limbic regions, such as the bed nucleus of the stria terminalis (BST). The route of this reciprocal connection, in particular with the BST, remains unknown. In order to visualize this pathway, we used the fluorescent tracer carbocyanine dye (DiI), that was inserted in the arcuate nucleus in fixed and dissected brains. This allowed us to label an arcuate-BST pathway DiI-labeled coursing through the stria terminalis. Immunohistochemistry for the arcuate-derived peptide adrenocortico-tropin (ACTH) revealed the presence of ACTH-immunoreactive axons in the stria terminalis. Together, these results provide arguments in favour of the existence of an arcuatofugal projection to the BST via the stria terminalis.
authors.affiliation: Laboratoire de Neurocytochimie Fonctionnelle, URA CNRS 339, Université de Bordeaux I, Talence, France.
authors.forename: R
authors.initials: R
authors.lastname: Magoul
authors.forename: P
authors.initials: P
authors.lastname: Ciofi
authors.forename: G
authors.initials: G
authors.lastname: Tramu
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000276:immunology / Q000378:metabolism; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000502:physiology*; D001369:Axons / Q000378:metabolism; D002232:Carbocyanines; D004525:Efferent Pathways / Q000166:cytology / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D011333:Pro-Opiomelanocortin / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013787:Thalamic Nuclei / Q000166:cytology / Q000502:physiology*
chemical_list: D002232:Carbocyanines; C024286:3,3'-dioctadecylindocarbocyanine; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone