A 70-kDa heat shock cognate protein suppresses the defects caused by a proteasome mutation in Saccharomyces cerevisiae.
An allele of mutation in the proteasome subunit gene Y7, y7-1, caused a temperature-sensitive growth in S. cerevisiae. One of the multi-copy suppressor genes for this growth defect was identical to SSB1, which encodes a 70-kDa heat shock cognate protein of the yeast. Introduction of the multi-copy SSB1 gene into the y7-1 mutant cells suppressed defects in the degradation of X-beta-galactosidase (X = Arg or Pro) observed in the mutant cells. Thus, the SSB1 protein, one of the chaperons of the yeast, facilitated intracellular protein degradation.
Ohba
M
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Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
D000595:Amino Acid Sequence; D002451:Cell Compartmentation; D002455:Cell Division / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics*; D003600:Cytosol / Q000737:chemistry; D005656:Fungal Proteins / Q000235:genetics*; D019204:GTP-Binding Proteins; D005800:Genes, Fungal / Q000235:genetics; D005804:Genes, Lethal / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D009154:Mutation; D010445:Peptide Elongation Factors; D046988:Proteasome Endopeptidase Complex; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013489:Suppression, Genetic
D005656:Fungal Proteins; C077247:HBS1 protein, S cerevisiae; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D009097:Multienzyme Complexes; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D019204:GTP-Binding Proteins