Priming to heat shock proteins in infants vaccinated against pertussis.
To investigate whether and in which proportion normal individuals experience a priming to microbial heat shock proteins (hsp), the presence of antibodies to two mycobacterial hsp was tested in serum sample from 2- to 4-mo-old children before and at different times after vaccination with the trivalent vaccine against tetanus, diphtheria, and pertussis (DTP). We show that 88.9% of infants vaccinated with DTP developed antibody responses to mycobacterial hsp. Such a response was due to the whole-cell pertussis component of the vaccine, because it was not observed in infants receiving an acellular pertussis vaccine. Antibodies and cells reactive to the mycobacterial 65-kDa hsp were also found in mice immunized with DTP. Interestingly, whole-cell pertussis vaccine-induced anti-hsp antibodies cross-reacted with the Escherichia coli GroEL hsp, and at a some extent with the human 60-kDa hsp, belonging to the same hsp family. These data suggest that priming of the immune system to hsp is a common phenomenon occurring very early in life.
Del Giudice
G
G
Department of Pathology, University of Geneva, Switzerland.
Gervaix
A
A
Costantino
P
P
Wyler
C A
CA
Tougne
C
C
de Graeff-Meeder
E R
ER
van Embden
J
J
van der Zee
R
R
Nencioni
L
L
Rappuoli
R
R
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology; D018834:Chaperonin 60; D003429:Cross Reactions; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology; D006360:Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007223:Infant; D051379:Mice; D008807:Mice, Inbred BALB C; D010567:Pertussis Vaccine / Q000276:immunology*; D014611:Vaccination
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D006360:Heat-Shock Proteins; D007074:Immunoglobulin G; D010567:Pertussis Vaccine