title: Apoptosis associated DNA fragmentation in epileptic brain damage.
abstract: We have examined the epileptic brain damage generated by intra-amygdaloid administration of kainic acid (KA). In the vulnerable CA3 region of the rat hippocampus, neurones developed apoptotic properties since, (i) their nuclei were positively stained with a selective in situ DNA fragmentation staining method; (ii) DNA cleavage into internucleosome-sized fragments was observed on agarose gels. Our results suggest that epileptic seizures generated in the amygdala by KA trigger apoptotic cell damage in pyramidal CA3 neurones.
authors.affiliation: INSERM U 29, Laboratoire de Neurobiologie et Physiopathologie du Développement, Hôpital de Port Royal, Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Pollard
authors.forename: S
authors.initials: S
authors.lastname: Cantagrel
authors.forename: C
authors.initials: C
authors.lastname: Charriaut-Marlangue
authors.forename: J
authors.initials: J
authors.lastname: Moreau
authors.forename: Y
authors.initials: Y
authors.lastname: Ben Ari
mesh_terms: D000679:Amygdala; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001927:Brain Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D006624:Hippocampus / Q000473:pathology; D007267:Injections; D007608:Kainic Acid / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004247:DNA; D007608:Kainic Acid