Structural features of LPPG and related compounds isolated from Trypanosoma cruzi trypomastigotes.
A hydrophobic fraction isolated from trypomastigotes of Trypanosoma cruzi is being characterized using immunological and chemical techniques. The lipopeptidophosphoglycan (LPPG) was identified in this fraction since it gave a positive reaction with anti-LPPG rabbit serum and had similar structural features such as the presence of ceramide as the lipid moiety, furanoic galactose, and a glycan moiety consistent with that obtained from an authentic sample of epimastigote LPPG, as judged by thin-layer chromatography. Furthermore, the hydrophobic fraction contained other glycolipids with different structural features. The lipid moiety of these compounds is alkylglycerol rather than a ceramide, the carbohydrate chain appears to be less complex than that in LPPG and no reactivity was observed towards an anti-LPPG serum.
Casal
O L
OL
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Zingales
B
B
Colli
W
W
D000818:Animals; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D006001:Glycoconjugates / Q000737:chemistry; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D015800:Protozoan Proteins / Q000737:chemistry*; D014349:Trypanosoma cruzi / Q000737:chemistry*
D006001:Glycoconjugates; D010457:Peptidoglycan; D010743:Phospholipids; D015800:Protozoan Proteins