Ascorbic acid increases synaptosomal potassium-induced dopamine release.
On synaptosomes prepared from striata of mice, increasing concentrations of ascorbic acid (from 0.01 mM to 0.5 mM) did not modify the 3H-dopamine uptake. However, at the 0.1 mM concentration, ascorbic acid increased the potassium-induced release of 3H-dopamine by synaptosomes previously loaded with the amine. This effect was dependent on the presence of Ca2+ in the superfusion medium and was not shared by dehydroascorbic acid (from 1 mM to 0.01 mM). This effect of ascorbic acid, which occurs in the range of its endogenous concentrations, suggests that it is a putative modulator of dopaminergic transmission.
Girbe
F
F
Unité de Neuropsychopharmacologie, CNRS URA 1170, Faculté de Médecine et Pharmacie de Rouen, Saint-Etienne du Rouvray, France.
Ramassamy
C
C
Piton
C
C
Costentin
J
J
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D003683:Dehydroascorbic Acid / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D001205:Ascorbic Acid; D011188:Potassium; D004298:Dopamine; D003683:Dehydroascorbic Acid