Age dependent changes in mitochondrial FoF1 ATP synthase in regenerating rat-liver.
The effect of aging on rat liver regeneration and on the FoF1-ATP synthase complex of isolated liver mitochondria was followed after partial (70%) hepatectomy. ATP hydrolase activity in submitochondrial particles prepared from regenerating liver was first depressed; the time needed to reach the lowest activity was age dependent. This decrease was accompanied by parallel decrease of i) the respiratory rate of succinate supplemented mitochondria in state III; ii) the respiratory control index; iii) the rate of synthesis of ATP in succinate supplemented submitochondrial particles. This first phase of liver regeneration, characterized at all ages by a lag phase in the growth, was followed by a second phase in which the tissue mass was restored and the enzyme activities normalized. Immunoblot analysis showed that the changes in the catalytic activities of the FoF1-ATP synthase observed during liver regeneration were accompanied by parallel changes in the amount of subunits of both the catalytic (F1) and the membrane (Fo) sector of the complex.
Guerrieri
F
F
Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
Kalous
M
M
Capozza
G
G
Muolo
L
L
Drahota
Z
Z
Papa
S
S
D000375:Aging / Q000378:metabolism*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006498:Hepatectomy; D015151:Immunoblotting; D008115:Liver Regeneration; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D009929:Organ Size; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D006180:Proton-Translocating ATPases