Effects of Plasmodium berghei (Apicomplexa) on Nippostrongylus brasiliensis (Nematoda) infection in the mouse, Mus musculus.
Ova and free oxygen radical production and relative peripheral eosinophilia during single and concurrent infections in mice with P. berghei and N. brasiliensis were investigated. Prolonged helminth patent periods indicate that Nippostrongylus self-cure in concurrently infected mice was suppressed. Differential white blood cell determinations showed that the relative number of peripheral blood eosinophils steadily increased (P < or = 0.05) during a Nippostrongylus infection when compared to noninfected controls. Eosinophil levels in mice singly infected with P. berghei or concurrently infected with both parasites did not differ significantly from those of controls suggesting a suppression by Plasmodium of Nippostrongylus-induced eosinophilia. Generation of intestinal free oxygen radicals was indirectly assessed using the Thiobarbituric Acid Assay to measure malondialdehyde (MDA). Intestinal MDA levels were significantly elevated (P < or = 0.05) during single Plasmodium infections as well as concurrent infections while there was no change in MDA production during single Nippostrongylus infections. These results suggest suppression by Plasmodium of the immune response to Nippostrongylus, allowing prolonged patent periods. They also suggest that eosinophils play a role in self-cure while free oxygen radicals do not.
Modrić
S
S
Biological Sciences Department, University of Texas at El Paso 79968-0519.
Mayberry
L F
LF
D000818:Animals; D004802:Eosinophilia / Q000209:etiology; D005609:Free Radicals; D008288:Malaria / Q000150:complications*; D008297:Male; D051379:Mice; D009559:Nippostrongylus / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism; D010270:Parasite Egg Count; D010962:Plasmodium berghei / Q000502:physiology*; D017206:Strongylida Infections / Q000150:complications*
D005609:Free Radicals; D010100:Oxygen