Functional interaction of heat shock protein GroEL with an RNase E-like activity in Escherichia coli.
The highly specific endoribonuclease activities of RNase E (which processes ribosomal 9S RNA into p5S RNA) and RNase K (which initiates decay of the ompA mRNA) are inferred to play a central role in RNA processing and mRNA decay in Escherichia coli. In vivo both activities are affected by a conditional mutation of the ams/rne gene that seems to be complemented at nonpermissive temperatures by a fragment of the groEL gene. Analysis of the relationship between the two nucleases and the heat shock protein revealed that GroEL interacts functionally with an RNase E-like activity but not with an RNase K activity, a groEL mutation affected 9S RNA processing but not ompA mRNA cleavage, RNase E activity could be precipitated with an antibody against GroEL, and a highly purified GroEL preparation contained RNase E activity but not RNase K activity. When purifying RNase E activity, we obtained a preparation containing two major proteins of 60 and 17 kDa. The size and the N-terminal sequence identified the 60-kDa protein as GroEL.
Sohlberg
B
B
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden.
Lundberg
U
U
Hartl
F U
FU
von Gabain
A
A
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D017123:Bacteriophage T7 / Q000235:genetics; D018834:Chaperonin 60; D004722:Endoribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D014158:Transcription, Genetic
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D004722:Endoribonucleases; C065559:ribonuclease K; C022537:ribonuclease E