title: Expression of resistance genes in acute leukemia.
abstract: To evaluate the frequency and the prognostic value of different mechanisms of drug resistance in acute leukemias, we investigated the expression of mdr1 by immunocytochemistry, mRNA slot blot or RT-PCR in 182 cases of adult acute myeloid and 37 cases of adult lymphoblastic leukemia. Before treatment, 39% of de novo AML, 38% of secondary AML, and 7% of de novo ALL exhibited a high level of mdr1 mRNA. After chemotherapy, the frequency of mdr1 gene expression in ALL raised dramatically to 60% (P < 0.005), while no significant change was found for AML cases. In 91 patients treated with MDR-related drugs, mdr1 gene expression was related to the failure of chemotherapy (P < 0.0001). The overexpression of multidrug resistance-associated protein (mrp) and anionic glutathione S-transferase (GST pi) was also investigated in 38 and 61 AML patients respectively. An overexpression of mrp gene was noted in 39% of the cases. For GST pi gene, the frequency of overexpression was 28%. A positive and significative correlation was found among mdr1, mrp and GST pi genes expression.
authors.affiliation: Laboratoire de Cinétique et de Cultures Cellulaires, Formation de Recherche Associée Claude Bernard sur le Traitement des Hémopathies Malignes, Paris, France.
authors.forename: D C
authors.initials: DC
authors.lastname: Zhou
authors.forename: L
authors.initials: L
authors.lastname: Hoang-Ngoc
authors.forename: A
authors.initials: A
authors.lastname: Delmer
authors.forename: A M
authors.initials: AM
authors.lastname: Faussat
authors.forename: D
authors.initials: D
authors.lastname: Russo
authors.forename: R
authors.initials: R
authors.lastname: Zittoun
authors.forename: J P
authors.initials: JP
authors.lastname: Marie
mesh_terms: D014408:Biomarkers, Tumor; D004351:Drug Resistance / Q000235:genetics*; D015870:Gene Expression; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D005982:Glutathione Transferase