A trap system for the isolation of amino acid sequences inducing GPI anchor addition.
We designed a trap system to isolate different amino acid sequences which could target proteins to the cell surface via GPI anchor transfer. This selection procedure is based on the insertion of various sequences which regenerate a functional GPI anchor signal sequence and therefore provoke re-expression at the surface of a reporter molecule. Using this trap for cell surface targeting sequences, we could show the importance of the defined elements essential for GPI anchor addition. Such a system could be used for an exhaustive analysis of the carboxyl terminus structural requirements for GPI membrane anchoring.
Fasel
N
N
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Stark
H
H
Bron
C
C
Beghdadi-Rais
C
C
D000595:Amino Acid Sequence; D000954:Antigens, Surface / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D005434:Flow Cytometry; D005822:Genetic Vectors; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis*; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D021382:Protein Sorting Signals; D014162:Transfection
D000954:Antigens, Surface; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D010455:Peptides; D021382:Protein Sorting Signals