Modulation of biochemical and cytological profile of bronchoalveolar lavage constituents in rats following split-dose multiple inhalation exposure to methyl isocyanate.
1. Studies were carried out to explore the acute pulmonary effects of equal, split-dose, multiple inhalation exposures of rats to methyl isocyanate (MIC), (0.32 mg l-1, 8 min x 10 exposures) as reflected by alterations in bronchoalveolar lavage fluid (BALF) constituents and to evaluate recovery, if any, following survival in a MIC-free environment, 10 d after the last MIC exposure. 2. In the BALF of MIC-exposed rats, there was an increase in the total number of cells and the number of cells showing enhanced dye uptake and reduction of nitroblue tetrazolium chloride. The cell-free BALF showed increases in total protein, sialic acids and lactic acid contents and lactate dehydrogenase activity. 3. In rats exposed to MIC and sacrificed 10 d after survival in a MIC-free environment, there was a reduction in the cellular and biochemical constituents of BALF. The phagocytic potential of macrophages was, however, also decreased under this regime.
Gupta
G S
GS
Industrial Toxicology Research Centre, Lucknow, India.
Bajpai
R
R
Kaw
J L
JL
Dutta
K K
KK
Ray
P K
PK
D000280:Administration, Inhalation; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D003485:Cyanates / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D007509:Irritants / Q000633:toxicity; D017953:Isocyanates; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D011506:Proteins / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012794:Sialic Acids / Q000378:metabolism
D003485:Cyanates; D007509:Irritants; D017953:Isocyanates; D007773:Lactates; D011506:Proteins; D012794:Sialic Acids; D019344:Lactic Acid; C008461:methyl isocyanate