POU-domain genes in planarian Dugesia japonica: the structure and expression.
In fresh water planarian Dugesia japonica, two genes encoding POU-domain proteins, DjPOU1 and DjPOU2, have been identified by polymerase chain reaction (PCR). Their POU-domains are strikingly similar to class III POU-domains in mammals, frog, insects and nematode. The full-length cDNA of DjPOU1 has been cloned and sequenced, which revealed that it codes for a protein of 559 amino acids. The accumulation of DjPOU1 mRNA is two or three times more in anterior region of worm containing a brain, than the other regions, and decreases during regeneration after amputation.
Orii
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Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Park City, Hyougo, Japan.
Agata
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Watanabe
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K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D005796:Genes; D005801:Genes, Homeobox; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010932:Planarians / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D003062:Codon; D004247:DNA