Overproduction, isolation, and DNA-binding characteristics of Xre, the repressor protein from the Bacillus subtilis defective prophage PBSX.
PBSX is a phage-like bacteriocin (phibacin) of Bacillus subtilis 168. Lysogeny is maintained by the PBSX-encoded repressor, Xre. The Xre protein was overproduced in Escherichia coli and isolated by affinity chromatography. Gel retardation and DNase I footprinting studies indicated that Xre binds to four sites close to its own gene. These sites overlap putative promoters for xre and a divergent transcriptional unit, containing the middle genes.
McDonnell
G E
GE
Department of Genetics, Trinity College, Dublin, Ireland.
McConnell
D J
DJ
D017103:Bacillus Phages / Q000235:genetics*; D001412:Bacillus subtilis / Q000502:physiology*; D001430:Bacteriocins / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D004279:DNA, Viral / Q000378:metabolism; D003673:Defective Viruses / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008242:Lysogeny; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D009875:Operator Regions, Genetic / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D001430:Bacteriocins; D004279:DNA, Viral; D012097:Repressor Proteins; D014764:Viral Proteins