Antithrombotic activity of clove oil.
Clove oil inhibited human platelet aggregation induced by arachidonic acid (AA), platelet-activating factor (PAF) or collagen. Clove oil was a more effective inhibitor for aggregation induced by AA and PAF (IC50:4 and 6 microM respectively) than collagen (IC50: 132 microM). The in vivo experiments in rabbits showed that clove oil (50-100 mg/kg) afforded 100% protection against PAF (11 mg/kg, i.v.) and 70% protection against AA (2.0 mg/kg, i.v.)-induced thrombosis and shock due to pulmonary platelet thrombosis. It also inhibited thromboxane-A2 and 12-HETE production by human platelets incubated with [C14] AA. These results are indicative that clove oil is inhibitory of platelet aggregation and thromboxane synthesis and may act as anti-thrombotic agent.
Saeed
S A
SA
Department of Pharmacology, Aga Khan University Medical College, Karachi.
Gilani
A H
AH
D000818:Animals; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors; D001792:Blood Platelets / Q000378:metabolism; D005054:Eugenol / Q000494:pharmacology*; D008297:Male; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011655:Pulmonary Embolism / Q000139:chemically induced / Q000188:drug therapy; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013927:Thrombosis / Q000139:chemically induced / Q000188:drug therapy
D001095:Arachidonic Acids; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D005054:Eugenol