Combined effects of mercury and hexachlorobenzene in the rat.
The objective of the present study was to assess the potential interactive effects of two Great Lakes chemical contaminants, hexachlorobenzene (HCB) and mercury (HgCl2). Groups of 10 female Sprague-Dawley rats were administered by gavage single doses of HCB (400, 600 mg/kg b.w. in corn oil), HgCl2 (10.0, 12.5 mg/kg b.w. aqueous) or combinations of both followed by observation for clinical signs of toxicity for 14 days. Five animals from treatment groups died before the termination of the study; one animal each in 600 mg HCB, 400 mg HCB + 10 mg HgCl2, and 600 mg HCB + 10 mg HgCl2, and two animals in 600 mg HCB + 12.5 mg HgCl2. The surviving animals were necropsied at the termination of the study, and hematological, clinical chemistry, histopathological and tissue residue analyses were performed. Relative liver weights were increased in both low and high dose groups of HCB but not in animals treated with HgCl2 alone. Co-administration of HgCl2 did not alter the HCB effects on the liver weight of the animals. Serum cholesterol levels were increased in all the groups receiving HCB but not HgCl2. No interactive effects on other serum parameters were seen in animals administered with both chemicals. Mild to moderate morphological changes occurred in the liver, thyroid, thymus, ovary and bone marrow of rats exposed to HCB or HCB+HgCl2, and in the kidney of HgCl2 or HgCl2+HCB treated animals. More severe histological changes occurred in the groups receiving both chemicals. The histological effects appeared to be additive. It was concluded that co-administration with HCB and HgCl2 resulted in additive effects in some of the endpoints measured but no synergism or antagonism was observed.
Lecavalier
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PR
Environmental and Occupational Toxicology Division, Environmental Health Directorate, Ottawa, Ontario, Canada.
Chu
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I
Villeneuve
D
D
Valli
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VE
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D004347:Drug Interactions; D005260:Female; D006581:Hexachlorobenzene / Q000493:pharmacokinetics / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008627:Mercuric Chloride / Q000633:toxicity*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D010758:Phosphorus / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D010758:Phosphorus; D006581:Hexachlorobenzene; D008627:Mercuric Chloride; D002784:Cholesterol; D007770:L-Lactate Dehydrogenase