Short-chain phosphatidylcholines as superior detergents in solubilizing membrane proteins and preserving biological activity.
The solubilization of plasma and organelle membranes by diheptanoylphosphatidylcholine (DHPC) has been studied. This short-chain phosphatidylcholine is shown to act as a mild detergent, solubilizing effectively both kinds of membranes at DHPC concentrations of 10-20 mM (0.5-1%). The size of the resulting mixed protein-lipid-DHPC micelles ranges between 5 and 8 nm. The protein conformation and hence the enzymatic activity are well preserved over a rather large DHPC concentration range (up to 4-5 times the DHPC concentration required for solubilizing the membranes). Evidence is presented that short-chain phosphatidylcholines are superior to most detergents commonly used by biochemists. This is true not only regarding its excellent dispersing power on both phospholipid bilayers (Gabriel & Roberts, 1986) and biological membranes but also as to its capacity to preserve the native protein structure and hence enzymatic activity in the solubilized state. Due to its special properties DHPC lends itself very well not only to membrane solubilization but also to the purification of the solubilized membrane proteins and reconstitution of the proteins into simple lipid bilayers. Concerning the mechanism of membrane solubilization, evidence indicates that DHPC interacts primarily with the lipid bilayer of the membrane and not with the membrane proteins. DHPC solubilizes membranes by being distributed into the lipid bilayer and breaking it up. In the resulting small mixed micelles, the protein remains associated with its preferred intrinsic membrane lipids and is thus stabilized. The protein-intrinsic lipid complex is successfully shielded from unfavorable contacts with H2O by DHPC-intrinsic lipid interactions.
Kessi
J
J
Laboratorium für Biochemie, Eidgenössische Technische Hochschule Zürich, Switzerland.
Poirée
J C
JC
Wehrli
E
E
Bachofen
R
R
Semenza
G
G
Hauser
H
H
D001429:Bacteriochlorophylls / Q000737:chemistry; D003902:Detergents / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D008567:Membranes, Artificial; D008871:Microvilli / Q000737:chemistry; D009004:Monosaccharide Transport Proteins / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry; D012995:Solubility; D013394:Sucrase-Isomaltase Complex / Q000737:chemistry
D001429:Bacteriochlorophylls; D003902:Detergents; D008563:Membrane Lipids; D008565:Membrane Proteins; D008567:Membranes, Artificial; D009004:Monosaccharide Transport Proteins; D010713:Phosphatidylcholines; C038470:1,2-heptanoylphosphatidylcholine; D013394:Sucrase-Isomaltase Complex; D000254:Sodium-Potassium-Exchanging ATPase