title: Single-base sequencing for rapid screening of plasmids for inserts with known mutations and correct orientation.
authors.affiliation: University of Western Ontario, London, Canada.
authors.forename: M B
authors.initials: MB
authors.lastname: Coulter-Mackie
mesh_terms: D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase; D015224:Dideoxynucleosides; D006801:Humans; D009154:Mutation; D010957:Plasmids; D017422:Sequence Analysis, DNA; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics
chemical_list: D015224:Dideoxynucleosides; D004247:DNA; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase; D001619:beta-N-Acetylhexosaminidases