title: Isolation and characterization of 25 unique DNA markers for human chromosome 22.
abstract: Twenty-five single-copy anonymous DNA markers for human chromosome 22 were isolated. These markers were assigned to four different regions on the chromosome. Six markers recognize restriction fragment length polymorphisms. The relative positions of five of these polymorphic markers on the framework map of chromosome 22 were determined by linkage analysis. The sizes of the NotI fragments recognized by 22 markers were determined by pulsed-field gel analysis. The total length of the NotI fragments identified is at least 12 Mb, which represents about 20% of the entire chromosome.
authors.affiliation: Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
authors.forename: N A
authors.initials: NA
authors.lastname: van Biezen
authors.forename: R H
authors.initials: RH
authors.lastname: Lekanne Deprez
authors.forename: A
authors.initials: A
authors.lastname: Thijs
authors.forename: P
authors.initials: P
authors.lastname: Heutink
authors.forename: B A
authors.initials: BA
authors.lastname: Oostra
authors.forename: A H
authors.initials: AH
authors.lastname: Geurts van Kessel
authors.forename: E C
authors.initials: EC
authors.lastname: Zwarthoff
mesh_terms: D002460:Cell Line; D002892:Chromosomes, Human, Pair 22; D004247:DNA / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D005819:Genetic Markers; D004247:DNA