[The use of monoclonal antibodies in the study of ion transporting pumps].
The active ion transport is related to some vital functions for the normal eukaryotic cell metabolism. The study of transport mechanism and its regulation is a fundamental problem in cellular biology. The production of monoclonal antibodies (AcM) let us to have a probe, with large specificity, for the study of the localization, distribution and function of carrier proteins in the epithelium, as well as the study of its molecular structure, synthesis and assembling in the cell. The quality of a monoclonal antibody depends of the proper selection of each step to follow in the course of its production. In this article, we examine the strategy more currently used in the production of monoclonal antibodies and its direct use in the ionics pump's morphological, biochemical and physiological study.
Del Castillo
J
J
Lab. Fisiología Gastrointestinal, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas.
Thomas
L
L
D000818:Animals; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000737:chemistry; D005057:Eukaryotic Cells / Q000737:chemistry* / Q000648:ultrastructure; D017136:Ion Transport / Q000502:physiology; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000254:Sodium-Potassium-Exchanging ATPase
El uso de anticuerpos monoclonales en el estudio de las bombas transportadoras de iones.