Interleukin-1 favours the in vitro development of type 2 T helper (Th2) human T-cell clones.
The effects exerted by interleukin-1 (IL1) on the growth and differentiation of human Th1 and Th2 cells were examined. Neither IL1 nor the IL1 receptor antagonist (IL1ra) had detectable activity toward the antigen- or anti-CD3 antibody-induced proliferative response of already established type 1 T helper (Th1) or type 2 T helper (Th2) clones. Moreover, neither exogenous IL1 addiction to, nor neutralization of, endogenously produced IL1 in bulk cultures before cloning changed the Th1-like cytokine profile of PPD-specific T-cell lines. Likewise, IL1 addition in bulk culture before cloning did not significantly affect the Th2-like cytokine profile of Der.p.I-specific T-cell lines (Dermatophagoides pteronyssinus group I). However, Der.p.I-specific T-cell lines, derived in the presence of anti-IL1 Ab, IL1ra or the M-20 IL1 inhibitor, exhibited the reduced ability to produce IL4 and an increased ability to produce interferon gamma (IFN gamma). More importantly, Der.p.I-specific T-cell lines derived in the presence of IL1ra developed into Der.p.I-specific CD4+ T-cell clones showing a Th0/Th1-like, instead of a Th0/Th2-like, cytokine profile. These data suggest that IL1 is not required for the growth of already established human Th1 or Th2 CD4+ T-cell clones and has no regulatory effects on the in vitro development of Th1-like cells, but it plays a critical role in the development of Th2-like cells.
Manetti
R
R
Division of Clinical Immunology and Allergy, University of Florence, Italy.
Barak
V
V
Piccinni
M P
MP
Sampognaro
S
S
Parronchi
P
P
Maggi
E
E
Dinarello
C A
CA
Romagnani
S
S
D000818:Animals; D039741:Antigens, Dermatophagoides; D017252:CD3 Complex / Q000276:immunology; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D006969:Hypersensitivity, Immediate / Q000276:immunology; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000502:physiology*; D008213:Lymphocyte Activation; D008925:Mites / Q000276:immunology; D012795:Sialoglycoproteins / Q000494:pharmacology; D012882:Skin Tests; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology
D039741:Antigens, Dermatophagoides; D017252:CD3 Complex; D006023:Glycoproteins; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D012795:Sialoglycoproteins; D014373:Tuberculin