title: Growth inhibition of human prostatic carcinoma cell lines by serotonin antagonists.
abstract: Neuroendocrine (NE) differentiation within the primary prostate tumor has been correlated with tumor progression and shortened patient survival. Serotonin (5-hydroxytryptamine, 5-HT), a known mitogen, is found in most neuroendocrine cells of the human prostate. We have previously found that human prostatic carcinoma cell lines, PC-3, DU-145 and LNCaP, display certain NE characteristics. In this study, we have examined the effects of several subtype-selective 5-HT receptor antagonists on the growth of the three lines. Of these, the 5-HT1A antagonist pindobind had the most marked antiproliferative effect in vitro. Pindobind also had marked growth-inhibitory effects on the aggressive PC-3 cell line in vivo, in athymic nude mice. Radioligand binding studies indicated the presence of 5-HT binding sites on all three cell lines. Our results suggest that 5-HT is involved in the growth of prostate tumor cells and may serve as a target for treatment.
authors.affiliation: Department of Genitourinary Oncology, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
authors.forename: M
authors.initials: M
authors.lastname: Abdul
authors.forename: P E
authors.initials: PE
authors.lastname: Anezinis
authors.forename: C J
authors.initials: CJ
authors.lastname: Logothetis
authors.forename: N M
authors.initials: NM
authors.lastname: Hoosein
mesh_terms: D002455:Cell Division / Q000187:drug effects; D000081005:Cyclohexane Monoterpenes; D006801:Humans; D008297:Male; D010869:Pindolol / Q000031:analogs & derivatives / Q000494:pharmacology; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000473:pathology*; D011869:Radioligand Assay; D011985:Receptors, Serotonin / Q000032:analysis; D012702:Serotonin Antagonists / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000081005:Cyclohexane Monoterpenes; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; C084712:pindobind; D010869:Pindolol