Effect of interleukin-1 alpha on the growth of Mycobacterium microti within J774A.1 cells.
The effect of mouse recombinant interleukin-1 alpha on the intracellular growth of Mycobacterium microti in a murine macrophage cell line J774A.1 was investigated. Interleukin-1 alpha added after infection to the M. microti-infected macrophage monolayers enhanced the growth of M. microti in a concentration-dependent manner and this growth enhancement was abrogated by neutralization of interleukin-1 alpha with anti-interleukin-1 alpha antibody. Cyclic adenosine monophosphate level in J774A.1 cells was increased by the addition of interleukin-1 alpha. Addition of dibutyryl cyclic adenosine monophosphate to infected J774A.1 cells increased the number of intracellular bacteria in a concentration-dependent manner. These results suggest that interleukin-1 alpha acts as a growth enhancer for intracellular M. microti and the growth enhancing effect of interleukin-1 alpha may be due to enhanced cellular cyclic adenosine monophosphate level.
Majumder
S
S
Institute of Microbial Technology (CSIR), Chandigarh, India.
Gupta
R
R
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D009161:Mycobacterium / Q000254:growth & development* / Q000378:metabolism
D007375:Interleukin-1; D003994:Bucladesine; D000242:Cyclic AMP