title: Multimodality correlative study of canine brain tumors. Proton magnetic resonance spectroscopy, positron emission tomography, and histology.
abstract: OBJECTIVE
  Structural/functional relationships in an induced canine brain tumor were studied using proton-magnetic resonance spectroscopy (1H-MRS), positron emission tomography (PET), and histology.
  
  
  METHODS
  Proton-MRS and PET data of implanted canine brain tumors were correlated with quantitative analysis of the tissue composition within the MRS and PET regions of interest (ROIs). Linear regression analysis was employed to correlate the 1H-MRS and PET data with the percent tumor and the percent total lesion (comprising tumor plus associated pathology ie, edema, cysts, hemorrhage, inflammation) within the ROI.
  
  
  RESULTS
  Using 1H-MRS, N-acetyl aspartate concentrations were indirectly correlated with the amount of tumor (P = .058), as well as the amount of tumor plus associated pathology (P = .032) within the ROI. Total creatine concentrations were indirectly correlated with the amount of tumor and the amount of tumor plus associated pathology within the ROI (P < .05). Lactate concentrations were directly correlated with the amount of tumor (P = .053) and the amount of tumor plus associated pathology (P = .058) within the ROI. Using PET, Oxygen metabolic rates were indirectly correlated with the amount of tumor and with the amount of tumor plus associated pathology within the ROI (P < .05). Glucose metabolic rates were directly correlated with both the amount of tumor and with the amount of tumor plus associated pathology at P < .05. Proton-MRS measured concentrations of choline and PET measured values for blood flow, and oxygen extraction showed correlations with the amount of tumor and with the amount of tumor plus associated pathology at P > or = .08.
  
  
  CONCLUSIONS
  The PET and MRS data were complementary with respect to suggesting anaerobic glucose metabolism for the tumor. Unlike other tumors, no increase in choline was noted in the canine tumor.
authors.affiliation: Department of Radiology, Johns Hopkins University, School of Medicine, Baltimore, Maryland.
authors.forename: J H
authors.initials: JH
authors.lastname: Anderson
authors.forename: J D
authors.initials: JD
authors.lastname: Strandberg
authors.forename: D F
authors.initials: DF
authors.lastname: Wong
authors.forename: P S
authors.initials: PS
authors.lastname: Conti
authors.forename: P B
authors.initials: PB
authors.lastname: Barker
authors.forename: S J
authors.initials: SJ
authors.lastname: Blackband
authors.forename: J
authors.initials: J
authors.lastname: Hilton
authors.forename: T K
authors.initials: TK
authors.lastname: Natarajan
authors.forename: R F
authors.initials: RF
authors.lastname: Dannals
authors.forename: M A
authors.initials: MA
authors.lastname: Samphilipo
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000473:pathology*; D001932:Brain Neoplasms / Q000175:diagnosis*; D003847:Deoxyglucose / Q000031:analogs & derivatives; D004285:Dogs; D005462:Fluorine Radioisotopes; D019788:Fluorodeoxyglucose F18; D005910:Glioma / Q000175:diagnosis*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012044:Regression Analysis; D014055:Tomography, Emission-Computed
chemical_list: D005462:Fluorine Radioisotopes; D019788:Fluorodeoxyglucose F18; D003847:Deoxyglucose