title: Distribution of protein kinase C alpha and accumulation of extracellular Ca2+ during early dentin and enamel formation.
abstract: Activation of the protein kinase C (PKC)-related signal transduction system has been associated with phenotypic expression in a wide variety of cell types. In in vitro studies, it has often been activated by relatively small increases in the Ca2+ concentration ([Ca2+]) in the medium. The studies reported here explored the hypothesis that localized increases in the extracellular [Ca2+] and activation of the PKC-related pathway may be involved in early dentin and enamel formation. Whole-head, freeze-dried sections through the developing molars of 5-day-old rats were evaluated by methods that localized non-crystalline Ca2+. Immunohistochemical methods were adapted for use with the freeze-dried sections, and two monoclonal antibodies were used to localize PKC alpha in the formative cells of the developing teeth. Low concentrations of extracellular Ca2+ were observed in the early, unmineralized dentin in the area of ameloblast differentiation. Increased concentrations occurred at the point of initial dentin mineralization, immediately before the beginning of enamel matrix deposition. PKC alpha was localized in the differentiating odontoblasts, at the beginning of dentin matrix deposition. It was intensely localized in the distal borders of the pre-ameloblasts, and appeared to redistribute in the cells during ameloblast differentiation. These observations suggest that local increases in the extracellular [Ca2+] and the PKC signal transduction pathway may be involved in key inductions in the early stages of dentin and enamel formation.
authors.affiliation: Center for Oral Biology, Karolinska Institute, Huddinge, Sweden.
authors.forename: J W
authors.initials: JW
authors.lastname: Bawden
authors.forename: B
authors.initials: B
authors.lastname: Rozell
authors.forename: T
authors.initials: T
authors.lastname: Wurtz
authors.forename: N
authors.initials: N
authors.lastname: Fouda
authors.forename: L
authors.initials: L
authors.lastname: Hammarström
mesh_terms: D000565:Ameloblasts / Q000737:chemistry / Q000166:cytology; D000566:Amelogenesis / Q000502:physiology*; D053523:Amelogenin; D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000032:analysis / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D003743:Dental Enamel / Q000737:chemistry; D003746:Dental Enamel Proteins / Q000032:analysis / Q000378:metabolism; D003804:Dentin / Q000737:chemistry; D003810:Dentinogenesis / Q000502:physiology*; D007150:Immunohistochemistry; D009804:Odontoblasts / Q000166:cytology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D014074:Tooth Calcification / Q000502:physiology*
chemical_list: D053523:Amelogenin; C507201:Amelx protein, rat; D003746:Dental Enamel Proteins; D011493:Protein Kinase C; D002118:Calcium