Assignment of 112 microsatellite markers to 23 chromosome 11 subregions delineated by somatic hybrids: comparison with the genetic map.
Using a panel of 25 somatic cell hybrids, we have regionally localized 112 microsatellite markers generated by Généthon and assigned to chromosome 11. A genetic map of 74 of them was produced using linkage analysis of the eight largest CEPH (Centre d'Etude du Polymorphisme Humain) families. They could be ordered on chromosome 11 with an average distance of 2.1 cM. The tight correlation observed between the genetic order and the physical assignment of these microsatellites reinforces the genetic map data. These newly localized markers identified by the PCR method using a standardized protocol represent useful tools for mapping YAC clones and establishing YAC contigs and for studying genetic diseases or cancers associated with specific genes and/or germinal/somatic rearrangements of chromosome 11.
Couillin
P
P
INSERM U178, Villejuif, France.
Le Guern
E
E
Vignal
A
A
Fizames
C
C
Ravisé
N
N
Delportes
D
D
Reguigne
I
I
Rosier
M F
MF
Junien
C
C
van Heyningen
V
V
D000818:Animals; D001483:Base Sequence; D001506:Beckwith-Wiedemann Syndrome / Q000235:genetics; D001859:Bone Neoplasms / Q000235:genetics; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007938:Leukemia / Q000235:genetics; D008969:Molecular Sequence Data; D012377:Rodentia; D012512:Sarcoma, Ewing / Q000235:genetics; D012559:Schizophrenia / Q000235:genetics; D012984:Software; D017624:WAGR Syndrome / Q000235:genetics
D017931:DNA Primers; D005819:Genetic Markers; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific