title: Regulation of quail oviduct phospholipase A2 activity by estradiol.
abstract: The phospholipase A2 (PLA2) activity was measured in the oviduct of immature and estradiol benzoate (EB)-treated quails. The pH profiles demonstrate the presence of two PLA2 isoforms in the avian oviduct: a neutral isoform, optimally active at pH 7-7.5 and calcium independent, responsible for most of the hydrolytic activity in the immature oviduct and poorly stimulated by estradiol; and an alkaline isoform, optimally active at pH 8-9.5 and calcium dependent, with little activity in the immature tissue but markedly stimulated by EB. After EB injection, PLA2 activation occurs at first during the prereplicative period of oviduct cells (+172% at 6 h), it is dose dependent from 0.01 to 1 mg/kg EB and can be prevented by cycloheximide together with ornithine decarboxylase activation. Moreover, estradiol was inactive on cell-free extracts of immature oviducts. These results suggest that EB increases PLA2 activity through gene activation and de novo protein synthesis. The correlation between the early stimulation of PLA2 activity and the proliferation of oviduct cells is discussed.
authors.affiliation: Laboratoire de Physiologie-Pharmacodynamie, INSERM U.352, Villeurbanne, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Fayard
authors.forename: S
authors.initials: S
authors.lastname: Chanal
authors.forename: B
authors.initials: B
authors.lastname: Felouati
authors.forename: O
authors.initials: O
authors.lastname: Macovschi
authors.forename: M
authors.initials: M
authors.lastname: Lagarde
authors.forename: J F
authors.initials: JF
authors.lastname: Pageaux
authors.forename: C
authors.initials: C
authors.lastname: Laugier
mesh_terms: D000818:Animals; D002455:Cell Division; D003370:Coturnix; D004789:Enzyme Activation; D004958:Estradiol / Q000494:pharmacology*; D005187:Fallopian Tubes / Q000187:drug effects* / Q000201:enzymology*; D005260:Female; D006801:Humans; D007700:Kinetics; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2
chemical_list: D004958:Estradiol; D010741:Phospholipases A; D054467:Phospholipases A2