Bacillus subtilis FlhA: a flagellar protein related to a new family of signal-transducing receptors.
The Bacillus subtilis flhA gene lies in the major che/fla operon, a transcription unit that spans 26 kilobases (kb) of DNA. flhA encodes a 677-amino-acid polypeptide that is a strong candidate for an integral membrane protein. The sequence of FlhA displays substantial homology to a newly identified family of putative signal-transducing receptors that have been implicated in diverse cellular processes. FlhA is the first member of this family to be described from a Gram-positive bacterium. We demonstrate that flhA is a flagellar gene and that FlhA is required in trans for the formation of products from some, but not all, B. subtilis motility-related operons that are regulated by the sigma D form of RNA polymerase. We suggest that FlhA is a component of a signalling system that is involved with the formation of some flagellar gene products during the biosynthesis of the flagellum.
Carpenter
P B
PB
Department of Biochemistry, College of Medicine, University of Illinois, Urbana 61801.
Ordal
G W
GW
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002465:Cell Movement; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D016297:Mutagenesis, Site-Directed; D009876:Operon; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
D001426:Bacterial Proteins; C080688:FlhA protein, Bacteria; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins