MDL-101,562 blocks the stimulatory effects of bombesin and gastrin-releasing peptide on hypothalamic dopaminergic neurons.
The effects of central administration of bombesin, gastrin-releasing peptide (GRP) and a putative bombesin/GRP receptor antagonist [Phe8 psi[CH2S]Leu9]litorin (MDL-101,562) were determined on the activities of hypothalamic tuberoinfundibular and periventricular-hypophysial dopaminergic neurons in male rats. Dopaminergic neuronal activity was estimated by measuring concentrations of 3,4-dihydroxyphenylacetic acid (DOPAC) in the median eminence and the intermediate lobe of the posterior pituitary which contain terminals of these neurons. Intracerebroventricular injections of bombesin and GRP increased DOPAC concentrations in both the median eminence and intermediate lobe. MDL-101,562 had no effect on median eminence or intermediate lobe DOPAC concentrations per se, but blocked the stimulatory actions of bombesin and GRP on DOPAC concentrations in these regions. These results suggest that MDL-101,562 may be a useful pharmacologic tool in the study of the central actions of bombesin and related peptides.
Manzanares
J
J
Department of Pharmacology and Toxicology, Michigan State University, East Lansing 48824-1317.
Edwards
J V
JV
Lookingland
K J
KJ
Moore
K E
KE
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001839:Bombesin / Q000037:antagonists & inhibitors*; D004298:Dopamine / Q000502:physiology*; D019886:Gastrin-Releasing Peptide; D007276:Injections, Intraventricular; D008297:Male; D008473:Median Eminence / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology*; D010455:Peptides / Q000037:antagonists & inhibitors*; D010904:Pituitary Gland, Posterior / Q000187:drug effects / Q000378:metabolism; D051381:Rats
C089302:MDL 101562; D009842:Oligopeptides; D010455:Peptides; D015102:3,4-Dihydroxyphenylacetic Acid; D019886:Gastrin-Releasing Peptide; D001839:Bombesin; D004298:Dopamine