Lipogenic enzyme and apoprotein messenger RNAs in long-term primary culture of chicken hepatocytes.
Hepatocytes isolated from 9-week-old chickens were cultured in a serum-free, hormonally defined medium. Relative amounts of mRNAs coding for lipogenic enzymes (acetyl-CoA carboxylase, fatty acid synthase, delta 9 desaturase, malic enzyme) and apoproteins (apoprotein A1 and apoprotein B) were determined until the 12th day. beta-actin and albumin mRNA, as well as albumin secretion, were also assessed. Cellular metabolic activity appeared to be very low for the first days of culture, but increased after the 7th day. All the mRNAs studied, except for that of malic enzyme, were present from this time throughout the culture lifespan. The biological significance of the observed results and the relevance of this chicken hepatocyte culture system for long-term metabolic and genetic studies are discussed.
Douaire
M
M
Laboratoire de génétique, Institut National de la Recherche Agronomique, Rennes, France.
Belloir
B
B
Guillemot
J C
JC
Fraslin
J M
JM
Langlois
P
P
Mallard
J
J
D000103:Acetyl-CoA Carboxylase / Q000235:genetics; D000818:Animals; D001059:Apoproteins / Q000235:genetics*; D002478:Cells, Cultured; D002645:Chickens; D044943:Fatty Acid Desaturases / Q000235:genetics; D064429:Fatty Acid Synthases / Q000235:genetics; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013230:Stearoyl-CoA Desaturase; D013997:Time Factors
D001059:Apoproteins; D008055:Lipids; D012333:RNA, Messenger; D008291:Malate Dehydrogenase; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase