Morphological relationships between tyrosine hydroxylase-immunoreactive neurons and dopamine-beta-hydroxylase-immunoreactive fibres in dopamine cell group A15 of the sheep.
Double immunocytochemical labelling with antibodies raised against tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase was used on semi-thin sections of sheep hypothalamus to investigate possible morphological relationships between dopamine neurons of group A15 and noradrenaline afferents to this area. Dopamine-beta-hydroxylase-immunoreactive (IR) fibres were found in the close proximity of dendrites of TH-IR neurons. At electron microscopic level, single immunocytochemical staining with TH antibodies revealed the presence of synaptic contacts between labelled or unlabelled axon terminals and anti-TH labelled dendrites. These observations suggest that in the sheep, TH-IR neurons of group A15 are controlled by non-catecholaminergic and catecholaminergic afferents. Catecholamine inputs could contain either dopamine or noradrenaline. The hypothesis of noradrenaline inputs to A15 is strongly supported by the results obtained after double labelling on semi-thin sections. Tyrosine hydroxylase-immunoreactive perikarya and dendrites often seemed to be partly surrounded by glial processes. This latter observation suggests that the synaptic investment of these neurons might be controlled by glial cells.
Tillet
Y
Y
Unité de Neuroendocrinologie Sexuelle, INRA Station de Physiologie de la Reproduction, Nouzilly, France.
Thibault
J
J
D000818:Animals; D003712:Dendrites / Q000201:enzymology / Q000276:immunology; D004298:Dopamine / Q000502:physiology*; D004299:Dopamine beta-Hydroxylase / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009412:Nerve Fibers / Q000201:enzymology* / Q000276:immunology; D009474:Neurons / Q000201:enzymology* / Q000276:immunology; D012756:Sheep; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology / Q000378:metabolism*
D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D004298:Dopamine