Comparison of cytochrome P-450 content and conjugative enzymes in livers of camels (Camelus dromedarius), guinea-pigs (Cavia porcellus) and rats (Rattus norvegicus).
The activities of the conjugative enzymes, glutathione S-transferase and UDP-glucuronyl-transferase, have been measured in vitro in the livers of camels, guinea-pigs and rats. Some sex differences were observed in the levels of these conjugative enzymes. In rats and guinea-pigs, females had higher UDP-glucuronyltransferase activity than males. In camels, females had higher glutathione S-transferase activity than males. In these species, the cytochrome P-450 isozymes observed between the 50,000 and 60,000 mol. wt regions have been separated and characterized by SDS-polyacrylamide gel electrophoresis. Camels showed lower levels of all types of cytochrome P-450 isozymes, while guinea-pigs showed higher levels of most of these isozymes. In general, camels seemed to have the lowest drug-metabolizing enzyme activity when compared to rats and guinea-pigs.
Damanhouri
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ZA
Department of Pharmacology, Faculty of Medicine and Allied Sciences, King Fahad Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Tayeb
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D000818:Animals; D002162:Camelus / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D014453:Glucuronosyltransferase / Q000032:analysis; D005982:Glutathione Transferase / Q000032:analysis; D006168:Guinea Pigs; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats
D003577:Cytochrome P-450 Enzyme System; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase