The concomitant augmentation of urokinase-type plasminogen activator and collagenase-like proteinase activities in X-ray irradiated cells of a human metastatic carcinomatous line.
The enzymatic activities of uPA, and a collagenase-like proteinase in the post-nuclear fraction of cell homogenates of a metastatic carcinomatous cell line following X-ray irradiation were examined by the use of chromogenic substrates and by casein- or gelatin-containing zymographies and electrophoretic gel stained with avidin-conjugated peroxidase. Enhanced activities were observed in these cells, while those of 5'nucleotidase and Na(+)-K(+)-ATPase were attenuated. A partial purification and characterization of the collagenase showed that it was able to hydrolyze the heat-denatured type-I collagen more efficiently than the native one. The activation of both uPA and collagenase enables an efficient degradation of matrix barrier proteins. These findings suggest that following a certain dose range of X-ray irradiation, tumor cells may increase their ability to migrate and invade through the enhancement of uPA and collagenase activities.
Uchida
N
N
Department of Radiology, Shimane Medical University, Izumo, Japan.
Takahashi
K
K
Kasai
T
T
Ishida
T
T
Kwaan
H C
HC
D000595:Amino Acid Sequence; D002277:Carcinoma / Q000201:enzymology* / Q000556:secondary; D002470:Cell Survival / Q000528:radiation effects; D017364:Collagenases / Q000528:radiation effects*; D006801:Humans; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000528:radiation effects*
D014568:Urokinase-Type Plasminogen Activator; D017364:Collagenases