title: Elemental diet-induced bacterial translocation can be hormonally modulated.
abstract: BACKGROUND
  The authors have previously documented that feeding mice an elemental diet resulted in bacterial translocation (BT) that could be prevented by the provision of dietary fiber. To test whether the protective effect of fiber was related to the stimulation of trophic gut hormones, the effects of sandostatin and bombesin were tested.
  
  
  METHODS
  Mice fed either chow or the elemental diet were stratified into several groups and the ability of bombesin (10 micrograms/kg, tid) or sandostatin (100 micrograms/kg bid) to modulate BT was examined. After 14 days, mice were sacrificed and BT, cecal bacterial population levels, mucosal protein, and small bowel weight was measured. Segments of the ileum and jejunum were examined histologically.
  
  
  RESULTS
  Incidence of elemental diet-induced BT (75%) was reduced by fiber (9%) or the administration of bombesin (13%) (p < 0.01). Although sandostatin did not promote BT in chow-fed mice, it reversed the protective effect of fiber on BT (75%) (p < 0.01).
  
  
  CONCLUSIONS
  Elemental diet-induced bacterial translocation can be modulated hormonally and the beneficial effects of fiber on diet-induced BT appears to be hormonally mediated.
authors.affiliation: Department of Surgery, Louisiana State University Medical Center, Shreveport 71130-3932.
authors.forename: Y
authors.initials: Y
authors.lastname: Haskel
authors.forename: D
authors.initials: D
authors.lastname: Xu
authors.forename: Q
authors.initials: Q
authors.lastname: Lu
authors.forename: E
authors.initials: E
authors.lastname: Deitch
mesh_terms: D000818:Animals; D018407:Bacterial Physiological Phenomena; D001839:Bombesin / Q000494:pharmacology; D002432:Cecum / Q000187:drug effects / Q000382:microbiology / Q000473:pathology / Q000502:physiology; D004043:Dietary Fiber / Q000494:pharmacology; D004926:Escherichia coli / Q000502:physiology; D005526:Food, Formulated; D005768:Gastrointestinal Hormones / Q000502:physiology*; D007082:Ileum / Q000187:drug effects / Q000382:microbiology / Q000473:pathology / Q000502:physiology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000382:microbiology / Q000473:pathology / Q000502:physiology; D007422:Intestines / Q000187:drug effects / Q000382:microbiology* / Q000473:pathology / Q000502:physiology*; D007583:Jejunum / Q000187:drug effects / Q000382:microbiology / Q000473:pathology / Q000502:physiology; D008297:Male; D051379:Mice; D015282:Octreotide / Q000494:pharmacology; D013004:Somatostatin / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms
chemical_list: D004043:Dietary Fiber; D005768:Gastrointestinal Hormones; D013004:Somatostatin; D001839:Bombesin; D015282:Octreotide