title: Hepatic and intestinal cytochrome P-450, glutathione-S-transferase and UDP-glucuronosyl transferase are affected by six types of dietary fiber in rats inoculated with human whole fecal flora.
abstract: The effects of six different sources of dietary fiber (inulin, wheat brain, carrot, cocoa, pea and oat fiber) on hepatic and intestinal cytochrome P-450 (EC 1.14.14.1), glutathione-S-transferase (GSH-T, EC 2.5.1.18) and UDP-glucuronosyl transferase (UDPG-T, EC 2.4.1.17) were studied using germ-free F344 rats subsequently inoculated with a human whole fecal flora. In the liver, the total concentration of P-450 was significantly lower in the wheat bran-fed group than in the carrot-fed group. The 2E1 form of P-450, involved in nitrosamine metabolism, was enhanced in the carrot-fed group compared with those fed most other types of fiber. Compared with the pea-fed group, rats fed cocoa had a lower constitutive 2C11 form and a higher 1A2 form. A very high concentration of small intestinal 1A1 form--involved in "toxication" reactions--was observed in rats fed cocoa. The specific activity of hepatic GSH-T was significantly higher in rats fed inulin than in all other groups, except the carrot-fed group. In the colon, GSH-T specific activity was twice as high in the oat-fed group as in the wheat bran-fed counterpart. Small intestinal GSH-T activity and hepatic and intestinal UDPG-T activities were unaffected by diet. Results are discussed in relation to potential health benefits.
authors.affiliation: INRA, UEPSD, Jouy-en-Josas, France.
authors.forename: N
authors.initials: N
authors.lastname: Roland
authors.forename: L
authors.initials: L
authors.lastname: Nugon-Baudon
authors.forename: J P
authors.initials: JP
authors.lastname: Flinois
authors.forename: P
authors.initials: P
authors.lastname: Beaune
mesh_terms: D000328:Adult; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D004043:Dietary Fiber; D005243:Feces / Q000382:microbiology*; D005856:Germ-Free Life; D014453:Glucuronosyltransferase / Q000032:analysis; D005982:Glutathione Transferase / Q000032:analysis; D006801:Humans; D007422:Intestines / Q000201:enzymology*; D007527:Isoenzymes / Q000032:analysis; D008099:Liver / Q000201:enzymology*; D008297:Male; D008954:Models, Biological; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D004043:Dietary Fiber; D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase