Molecular cloning, sequencing and expression of an isoform of cardiac alpha-tropomyosin from the Mexican axolotl (Ambystoma mexicanum).
The cDNA for alpha-tropomyosin (TM) was cloned by the polymerase chain reaction (PCR) from a lambda gt11 library constructed with mRNA from juvenile axolotl heart tissues. Subsequently, the nucleotide sequence of the cDNA was determined. This is the first reported cDNA for axolotl alpha-tropomyosin. Comparative analyses of the deduced amino acid sequence of this cDNA with Xenopus skeletal muscle alpha-tropomyosin sequences indicate that the axolotl heart cDNA has 93% and 96% homology in the regions of amino acids 39-80 (exon 2b) and 258-284 (exon 9a), respectively. However, there is about 86.55% homology at the nucleic acid level (coding region) and 97.5% homology at the amino acid level with that of Xenopus skeletal muscle alpha-tropomyosin cDNA. Northern blot analyses with polyA+ RNA from juvenile heart suggest the presence of at least two different transcripts for alpha-tropomyosin in axolotl heart. The results of 3'-RACE concur with those of northern blot analyses.
Luque
E A
EA
Department of Anatomy and Cell Biology, SUNY Health Science Center at Syracuse 13210.
Lemanski
L F
LF
Dube
D K
DK
D000558:Ambystoma mexicanum; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D015870:Gene Expression; D015723:Gene Library; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D011784:Quail; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014335:Tropomyosin / Q000096:biosynthesis*; D014981:Xenopus
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D014335:Tropomyosin