Physical mapping of inhibin beta-A in domestic cattle.
The gene for the beta-A subunit of inhibin (INHBA) was assigned to bovine syntenic group U13 by bovine x rodent hybrid somatic cells and the polymerase chain reaction (PCR). A 482-bp PCR fragment was used to clone a 37-kb cosmid. This cosmid was assigned to bovine Chromosome (Chr) 4 (BTA 4) by fluorescence in situ hybridization (FISH). This is the first assignment of a U13 marker to a bovine chromosome. A restriction fragment length polymorphism (RFLP) was detected with PstI within the INHBA cosmid.
Neibergs
H L
HL
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843.
Gallagher
D S
DS
Georges
M
M
Sargeant
L S
LS
Dietz
A B
AB
Womack
J E
JE
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D006224:Cricetinae; D004277:DNA, Single-Stranded; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007265:Inhibins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D004277:DNA, Single-Stranded; D007265:Inhibins