[Elimination of natural antibodies in discordant xenografts].
The effect of various techniques to eliminate natural performed antibodies (IgM/IgG) in a discordant kidney xenograft model are reported. The combination of multiple plasmapheresis, splenectomy and immunosuppressive drugs allowed to prolong kidney xenograft survival until three weeks when without treatment such a graft would be rejected within hours or minutes. To prevent the rebound of preformed antibodies, the association of multiple plasmapheresis and anti-IgM monoclonal antibodies seem to be efficient in such a model. The combination of a pig liver perfusion, a sublethal total body irradiation, a bone marrow graft and various drugs prolonged kidney xenograft survival up to 2 weeks with some signs of peripheral microchimerism. Advantages and disadvantages of each techniques are discussed.
Gianello
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P
D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D001803:Blood Transfusion / Q000379:methods; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D016030:Kidney Transplantation / Q000276:immunology / Q000379:methods*; D010956:Plasmapheresis / Q000379:methods*; D011323:Primates; D013156:Splenectomy / Q000379:methods*; D013552:Swine; D014183:Transplantation, Heterologous / Q000276:immunology*
D007075:Immunoglobulin M; D007166:Immunosuppressive Agents
Eliminationdes anticorps naturels dans les xénogreffes discordantes.