title: Endothelin-1 as a local ovarian regulator in porcine granulosa cells.
abstract: This study was conducted to investigate whether endothelin-1 (ET-1) has direct effects on the functions of porcine granulosa cells harvested from small or medium follicles. Positive ET-1-like immunoreactivity (ET-1-LI) was observed in the cultured porcine granulosa cells, and Northern blot analysis demonstrated the expression of mRNA for prepro-ET-1 in these cells. The binding study showed the presence of a nonselective, single class of binding sites which predominantly included the subtype ETB. Increased ET-1-LI was detected in human follicular fluid obtained from the patients with stimulated ovarian cycles. ET-1 stimulated basal and follicle-stimulating hormone (FSH)-stimulated secretion of progesterone during short-term incubation (2 h), while it inhibited FSH- and human-chorionic-gonadotropin-stimulated accumulation of progesterone during long-term incubation (48 h) of immature or moderately mature granulosa cells. ET-1 significantly increased DNA synthesis in the cells. These biological actions were induced by ET-1, probably via a cAMP-protein kinase A pathway and intracellular calcium mobilization-protein kinase C pathway. The results suggest that ET-1 exerts autocrine/paracrine effects on porcine granulosa cell functions.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Kamada
authors.forename: T
authors.initials: T
authors.lastname: Aso
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D015152:Blotting, Northern; D002452:Cell Count; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D019332:Endothelin-1; D016232:Endothelins / Q000032:analysis / Q000235:genetics / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015571:Follicular Fluid / Q000737:chemistry; D015870:Gene Expression; D006107:Granulosa Cells / Q000187:drug effects / Q000502:physiology*; D010053:Ovary / Q000502:physiology*; D011374:Progesterone / Q000096:biosynthesis / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013552:Swine
chemical_list: D006063:Chorionic Gonadotropin; D019332:Endothelin-1; D016232:Endothelins; D011498:Protein Precursors; D012333:RNA, Messenger; D011374:Progesterone; D005640:Follicle Stimulating Hormone; D004247:DNA