Uptake and release of beta-alanine in cerebellar granule cells in primary culture: regulation of release by glutamatergic and GABAergic receptors.
The uptake and release of beta-[3H]alanine were studied in cultured glutamatergic cerebellar granule cells of the rat. The uptake of beta-alanine was saturable and sodium-dependent, comprising one high-affinity transport component. It was inhibited by hypotaurine, taurine, GABA and homotaurine but not by glycine or glutamate. The release was enhanced by homoexchange, veratridine and high K+ concentrations (50 mM). The K(+)-stimulated release was at least partially Ca(2+)-dependent. The release was shown to be subject to regulation by GABAA receptors and glutamate receptors of the kainate type. The results signify that beta-alanine may have a functional role in cerebellar granule cells.
Saransaari
P
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Department of Biomedical Sciences, University of Tampere, Finland.
Oja
S S
SS
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D011810:Quinoxalines / Q000494:pharmacology; D016318:Quisqualic Acid / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology*; D017470:Receptors, Glutamate / Q000187:drug effects / Q000502:physiology*; D014701:Veratridine / Q000494:pharmacology; D015091:beta-Alanine / Q000378:metabolism*
D011810:Quinoxalines; D011963:Receptors, GABA-A; D017470:Receptors, Glutamate; D015091:beta-Alanine; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D014701:Veratridine; D016318:Quisqualic Acid; D008274:Magnesium; D002118:Calcium