Genomic sequence sampling: a strategy for high resolution sequence-based physical mapping of complex genomes.
We present a simple and efficient method for constructing high resolution physical maps of large regions of genomic DNA based upon sampled sequencing. The physical map is constructed by ordering high density cosmid contigs and determining a sequence fragment from each end of every clone. The resulting map, which contains 30-50% of the complete DNA sequence, allows the identification of many genes and makes possible PCR amplification of virtually any part of the genome. We apply this strategy to the automated analysis of the genome of the primitive eukaryote Giardia lamblia and evaluate its applicability to the physical mapping and DNA sequencing of the human genome.
Smith
M W
MW
Molecular Genetics Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037.
Holmsen
A L
AL
Wei
Y H
YH
Peterson
M
M
Evans
G A
GA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016386:Chromosome Walking / Q000379:methods*; D003360:Cosmids; D016054:DNA, Protozoan / Q000235:genetics; D016678:Genome; D016829:Giardia lamblia / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D016054:DNA, Protozoan; D015800:Protozoan Proteins