Simplified high-performance liquid chromatographic method for propranolol and five metabolites in liver perfusate, rat serum and dog plasma.
A new method has been developed for the analysis of propranolol plus five major metabolites in perfusate from isolated liver preparations, and also in rat serum and dog plasma. The regional isomers of hydroxypropranolol are clearly separated within a total run time of less than 15 min. The basic and neutral metabolites are extracted and analysed together, while the acidic metabolites are extracted in a second step. The new assay is more simple and time efficient than previously published methods.
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College of Pharmacy, University of Saskatchewan, Saskatoon, Canada.
Xia
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D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D005966:Glucuronidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006900:Hydroxylation; D008099:Liver / Q000378:metabolism*; D011433:Propranolol / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats
D011433:Propranolol; D005966:Glucuronidase