Isolation, identification and characterization of the FUN12 gene of Saccharomyces cerevisiae.
We have cloned and characterized the FUN12 gene which is found on chromosome 1 of Saccharomyces cerevisiae. The complete nucleotide (nt) sequence of the cDNA and the genomic clones shows that FUN12 is expressed as a 3.7-kb message and should encode a 97 kDa-protein. Immunoprecipitations using antipeptide antibodies showed that the cells contain a Fun12p of this size. The databases contain no nt sequences that are homologous to FUN12 and no protein homologous to Fun12p. Gene disruption experiments showed that FUN12 is an essential gene.
Sutrave
P
P
ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, MD 21702-1201.
Shafer
B K
BK
Strathern
J N
JN
Hughes
S H
SH
D000595:Amino Acid Sequence; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics* / Q000502:physiology; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D005656:Fungal Proteins