title: Identification of the hutUH operator (hutUo) from Klebsiella aerogenes by DNA deletion analysis.
abstract: Expression of Klebsiella aerogenes histidine utilization operons hutUH and hutIG is negatively regulated by the product of hutC. Multiple copies of the hutUH promoter region [hut(P)] present in trans were able to titrate the limited amount of host-encoded hut repressor (HutC). Thus, the hut(P) region contains a specific binding site for HutC. To identify DNA sequences required for HutC titration, we constructed and characterized a set of 40 left-entering and 28 right-entering deletions within a 250-bp DNA sequence containing the hut(P) region. Mutants carrying deletions that altered a unique dyad symmetric sequence, ATGCTTGTATAGACAAGTAT, from -11 to -30 relative to the hutUH promoter (hutUp) were unable to titrate hut repressor; mutants carrying deletions that left this sequence intact retained their ability to titrate hut repressor. Thus, we identify ATGCTTGT ACAAGTAT as the hutUH operator.
authors.affiliation: Department of Biology, University of Michigan, Ann Arbor 48109-1048.
authors.forename: R
authors.initials: R
authors.lastname: Osuna
authors.forename: A
authors.initials: A
authors.lastname: Schwacha
authors.forename: R A
authors.initials: RA
authors.lastname: Bender
mesh_terms: D001426:Bacterial Proteins; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D005090:Exodeoxyribonucleases; D015964:Gene Expression Regulation, Bacterial; D006639:Histidine / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000378:metabolism*; D015183:Restriction Mapping; D017384:Sequence Deletion
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C089120:HutC protein, Klebsiella aerogenes; D012097:Repressor Proteins; D006639:Histidine; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III