title: GP49, an invariant GPI-anchored antigen of Giardia lamblia.
abstract: Giardia lamblia is a primitive protozoan and a major cause of waterborne enteric disease throughout tropical and temperate zones. The ability to grow the infective trophozoites in culture as well as the discovery of the method of in vitro encystation made it possible to study the biology of this primitive protozoan and to characterize the surface antigens. Giardia trophozoites are exposed to high concentrations of fatty acids in the human small intestine. This raises the possibility that intestinal fatty acids may become incorporated into Giardia. Therefore, we determined the pattern of fatty acylation of Giardia surface molecules. By metabolic labeling with radiolabeled fatty acids we identified a single glycosylphosphatidylinositol (GPI)-anchored surface protein in Giardia. GP49 differs from the cysteine-rich variable surface antigens described previously. The presence of a GPI anchor in GP49 was supported by the metabolic incorporation of [14C]-ethanolamine, [3H]-myoinositol and fatty acids into the protein. This was confirmed by chemical and enzymatic cleavage experiments. Most interestingly, GP49 was found to be present in different isolates of Giardia and thus can be considered as an invariant surface antigen. Although the biological function of GP49 is not known, recently we have found that intact and soluble GP49 altered the electrolyte fluxes which regulate fluid secretion in the cultured human intestinal epithelial cell line, T84. These studies indicate that the GPI-anchored invariant antigen of Giardia may play an important role in the pathophysiology of giardiasis.
authors.affiliation: Department of Biology, University of Texas, El Paso 79968.
authors.forename: S
authors.initials: S
authors.lastname: Das
authors.forename: A
authors.initials: A
authors.lastname: Traynor-Kaplan
authors.forename: U
authors.initials: U
authors.lastname: Kachintorn
authors.forename: S B
authors.initials: SB
authors.lastname: Aley
authors.forename: F D
authors.initials: FD
authors.lastname: Gillin
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000737:chemistry; D000954:Antigens, Surface / Q000032:analysis* / Q000737:chemistry; D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism; D016829:Giardia lamblia / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D010740:Phospholipases / Q000378:metabolism; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D005227:Fatty Acids; D017261:Glycosylphosphatidylinositols; D014643:Variant Surface Glycoproteins, Trypanosoma; D010740:Phospholipases