Diabetes-induced reduction of neuronal survival in hypotonic environments in culture.
Dorsal root ganglion (DRG) neurons from streptozotocin (STZ)-induced diabetic and normal C57BL mice were exposed to three different hypotonic environments (1/2, 1/4, and 1/8 osmolar solutions). After rapid applications of these hypotonic solutions to the neurons, the cell volume autoregulatory mechanism operated in 1/2 osmolar solution but was disrupted in superhypotonic solutions below 1/4 osmolar in both kinds of mice. None of the neurons could survive 12 h after treatment with superhypotonic solutions. On the other hand, a gradual reduction of osmolarity of the culture medium enabled neurons in the normal mice to survive in 1/2 and 1/4 osmolar solutions as well as in an isotonic solution. However, this reduction of osmolarity increased neuronal cell death in the diabetic mice. These results suggest that the ability of DRG neurons to survive in hypotonic environments in culture may be lost in diabetes.
Sango
K
K
Department of Physiology, School of Medicine, Yokohama City University, Japan.
Horie
H
H
Takano
M
M
Inoue
S
S
Takenaka
T
T
D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects; D007038:Hypotonic Solutions; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure
D003470:Culture Media; D007038:Hypotonic Solutions