Effects of fetal forebrain transplants in ibotenic-injured nucleus basalis: an anatomical investigation.
Survival of fetal basal forebrain transplant (TP) into ibotenic-injured nucleus basalis of rats was examined after a delay lesion and TP (1 or 2 weeks) and a delay between harvest and TP (1-4.5 hours). Optimal TP survival occurred for TP made 2 weeks postlesion and less than 2 hours after harvesting. In these cases large, healthy TP-neurons displayed robust cytochrome oxidase (CO) activity and sent cholinergic processes throughout the TP and occasionally into host tissue. A mild astrocytic reaction was observed within the TP and at the host-TP interface. Surviving TPs increased choline acetyltransferase innervation and CO activity within the ipsilateral frontoparietal cortex. Therefore data suggest that fetal cholinergic TPs into the damaged NBM reduced neuronal degeneration within the NBM and stimulated remaining neurons spared by the lesion.
Lescaudron
L
L
Université de Nantes, Faculté des Sciences et des Techniques, CNRS URA 1340, Service de Physiologie Animale, Nantes, France.
Sutton
R L
RL
Stein
D G
DG
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism* / Q000637:transplantation; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein; D006085:Graft Survival; D007051:Ibotenic Acid / Q000009:adverse effects / Q000494:pharmacology*; D007150:Immunohistochemistry; D016548:Prosencephalon / Q000196:embryology* / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005904:Glial Fibrillary Acidic Protein; D007051:Ibotenic Acid; D003576:Electron Transport Complex IV; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase