title: Isolation of Tyr-W-MIF-1 from bovine hypothalami.
abstract: Tyr-W-MIF-1 (Tyr-Pro-Trp-Gly-NH2) was recently isolated from human brain cortex. We have now isolated it from bovine hypothalami by solid phase extraction and several consecutive rpHPLC steps monitored by an RIA originally developed for the endogenous brain peptide Tyr-MIF-1 (Tyr-Pro-Leu-Gly-NH2). Determination of the sequence of the purified material and comparison of its chromatographic behavior with synthetic Tyr-W-MIF-1 confirmed the structure. The synthetic peptide and the isolated material showed almost identical binding to mu opiate receptors.
authors.affiliation: VA Medical Center, New Orleans, LA.
authors.forename: L
authors.initials: L
authors.lastname: Hackler
authors.forename: A J
authors.initials: AJ
authors.lastname: Kastin
authors.forename: J
authors.initials: J
authors.lastname: Erchegyi
authors.forename: J E
authors.initials: JE
authors.lastname: Zadina
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D007031:Hypothalamus / Q000737:chemistry*; D009075:MSH Release-Inhibiting Hormone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000302:isolation & purification* / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000378:metabolism
chemical_list: D009479:Neuropeptides; D017450:Receptors, Opioid, mu; C077532:tyrosyl-prolyl-tryptophyl-glycinamide; D009075:MSH Release-Inhibiting Hormone