title: Tyrosine-rich acidic matrix protein (TRAMP) is a tyrosine-sulphated and widely distributed protein of the extracellular matrix.
abstract: Tyrosine-rich acidic matrix protein (TRAMP; 22 kDa extracellular matrix protein; dermatopontin) is a protein that co-purifies with lysyl oxidase and with dermatan sulphate proteoglycans, with possible functions in cell-matrix interactions and matrix assembly. Using a rabbit polyclonal antiserum raised against porcine TRAMP, which cross-reacts with both the human and murine forms of the protein, we show by immunoblotting that TRAMP has a widespread tissue distribution, including skin, skeletal muscle, heart, lung, kidney, cartilage and bone. In cultures of human skin fibroblasts, TRAMP incorporates both [35S]sulphate and [3H]tyrosine and is secreted into the medium, as shown by immunoprecipitation. Amino acid analysis of immunoprecipitated TRAMP demonstrates that many of the tyrosine residues in TRAMP are sulphated.
authors.affiliation: Department of Biochemistry, University of Edinburgh, UK.
authors.forename: E G
authors.initials: EG
authors.lastname: Forbes
authors.forename: A D
authors.initials: AD
authors.lastname: Cronshaw
authors.forename: J R
authors.initials: JR
authors.lastname: MacBeath
authors.forename: D J
authors.initials: DJ
authors.lastname: Hulmes
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D011233:Precipitin Tests; D013464:Sulfuric Acids / Q000378:metabolism; D013552:Swine; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; C082019:DPT protein, human; C504453:Dpt protein, mouse; D016326:Extracellular Matrix Proteins; D013464:Sulfuric Acids; D014443:Tyrosine