title: HIV infection of monocyte-derived macrophages in vitro reduces phagocytosis of Candida albicans.
abstract: HIV-1 infection of peripheral blood monocyte-derived macrophages (MDMs) is unrelated to the level of CD4 expression on the surface of the cell, is associated with considerable donor variability, causes minimal cytopathology, and results in peak viral antigen production after 2 weeks of infection. Phagocytosis of opsonized Candida albicans by MDMs infected in vitro with several strains of HIV was compared with that of uninfected cells from the same donors; the proportion of MDMs containing the fluorescein isothiocyanate-labeled yeast was determined by flow cytometry and phase contrast microscopy. The intracellular localization of C. albicans was confirmed by confocal microscopy. Using paired MDMs from nine donors, 81% of uninfected and 53% of HIV-infected MDMs phagocytosed C. albicans. In addition, the number of yeast per cell was significantly higher in uninfected MDMs than in HIV-infected cells (mean 6.1 versus 2.5). These findings may partially explain the high incidence of mucocutaneous candidiasis in HIV-infected patients with advanced disease.
authors.affiliation: National Centre for HIV Virology Research/AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Melbourne, Australia.
authors.forename: S M
authors.initials: SM
authors.lastname: Crowe
authors.forename: N J
authors.initials: NJ
authors.lastname: Vardaxis
authors.forename: S J
authors.initials: SJ
authors.lastname: Kent
authors.forename: A L
authors.initials: AL
authors.lastname: Maerz
authors.forename: M J
authors.initials: MJ
authors.lastname: Hewish
authors.forename: M S
authors.initials: MS
authors.lastname: McGrath
authors.forename: J
authors.initials: J
authors.lastname: Mills
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000453:epidemiology; D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D017252:CD3 Complex / Q000032:analysis / Q000502:physiology; D015704:CD4 Antigens / Q000032:analysis / Q000502:physiology; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000150:complications / Q000453:epidemiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D004198:Disease Susceptibility; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D015497:HIV-1 / Q000302:isolation & purification; D006801:Humans; D015994:Incidence; D008264:Macrophages / Q000382:microbiology* / Q000473:pathology* / Q000502:physiology; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D010587:Phagocytosis / Q000502:physiology*
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016650:Fluorescein-5-isothiocyanate