Determination of azelaic acid in pharmaceuticals and cosmetics by RP-HPLC after pre-column derivatization.
This paper reports a RP-HPLC method for the determination in topics of azelaic acid, a keratolytic and anti-comedogenic agent widely used in the treatment of all types of acne. A derivatization step was needed prior to chromatographic analysis because the analyte is lacking in chromophore. A sample clean-up procedure by solid-phase extraction was also developed to analyse azelaic acid in complex matrices, such as pharmaceutical and cosmetic formulations.
Ferioli
V
V
Dipartimento di Scienze Farmaceutiche, Università di Modena, Italy.
Rustichelli
C
C
Vezzalini
F
F
Gamberini
G
G
D000970:Antineoplastic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003358:Cosmetics / Q000032:analysis*; D003879:Dermatologic Agents / Q000032:analysis*; D003998:Dicarboxylic Acids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013055:Spectrophotometry, Infrared
D000970:Antineoplastic Agents; D003358:Cosmetics; D003879:Dermatologic Agents; D003998:Dicarboxylic Acids; C010038:azelaic acid