Amino acid alcohols: growth inhibition and induction of differentiated features in melanoma cells.
The effects of a series of D- and L-amino acid alcohols on the proliferation and phenotypic expression of B16 mouse melanoma cells were evaluated. B16 melanoma cells were incubated for different time intervals in the presence of D- or L-phenylalaninol (PHE), D- or L-alaninol (AL), D- or L-leucinol (LE), L-histidinol (HIS), L-tyrosinol (TYR) and L-methioninol (MET). All agents, including the D or L configuration, induced an anti-proliferative effect, although of considerably different magnitude. D-PHE was the most active growth inhibitor. The growth inhibitory effects were accompanied by phenotypic alterations, which included morphological changes and enhancement in the activities of NADPH cytochrome c reductase and tau-glutamyl transpeptidase. These phenotypic alterations correlated with the growth inhibitory effects of the different agents and seem to reflect a higher differentiated state.
Landau
O
O
Department of Surgery, Beilinson Medical Center, Petah Tiqva, Israel.
Wasserman
L
L
Deutsch
A A
AA
Reiss
R
R
Panet
H
H
Novogrodsky
A
A
Nordenberg
J
J
D000605:Amino Alcohols / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D008546:Melanoma, Experimental / Q000201:enzymology / Q000473:pathology*; D051379:Mice; D009251:NADPH-Ferrihemoprotein Reductase / Q000096:biosynthesis; D010641:Phenotype; D005723:gamma-Glutamyltransferase / Q000096:biosynthesis
D000605:Amino Alcohols; D009251:NADPH-Ferrihemoprotein Reductase; D005723:gamma-Glutamyltransferase