Expression of metallopanstimulin in condylomata acuminata of the female anogenital region induced by papilloma virus.
We have identified by cDNA cloning a gene, denoted MPS-1, that is activated in cultured human transformed cells by growth factors. The MPS-1 gene contains one zinc finger domain similar to those present in the C4 family of DNA binding proteins. In this study, the expression of MPS-1 mRNA and protein were examined in HPV-induced human condylomata acuminata. Initially, we detected the presence of MPS-1 mRNA by message amplification phenotyping in all condylomata tissues examined. Subsequently, the cellular distribution and abundance of MPS-1 mRNA was studied by in situ hybridization with specific MPS-1 DNA and RNA probes. We found that MPS-1 mRNA is expressed at high levels in the cytoplasm of condylomata cells. In contrast, the MPS-1 mRNA is expressed at low levels in nonneoplastic tissues. Moreover, antibodies were raised against the predicted N-terminal sequence of the MPS-1 protein and used to detect MPS-1 in condylomata cells. MPS-1 immunoreactivity was detected in the cytoplasm and/or the perinuclear regions of condylomata cells, with marked staining in areas of active proliferation. In distinction, MPS-1 immunoreactivity was very weak in normal epithelial cells. The results support the contention that the MPS-1 protein may be a potentially important mediator of proliferative responses induced by HPV.
Xynos
F P
FP
Laboratory of Molecular Oncology, DVA Medical Center, St. Louis, MO 63106.
Klos
D J
DJ
Hamilton
P D
PD
Schuette
V
V
Huygens
P
P
Fernandez-Pol
J A
JA
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001483:Base Sequence; D003218:Condylomata Acuminata / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D005260:Female; D005831:Genital Diseases, Female / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008667:Metalloproteins / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics*; D027383:Papillomaviridae; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D008667:Metalloproteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C083686:RPS27 protein, human; D012269:Ribosomal Proteins