Characterization of the 5'-regulatory region of rat Reg I gene.
We report here the characterization of the 5'-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic beta-cells. Transient expression assays of the 5'-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304/-237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256/-237 oligonucleotide.
Miyashita
H
H
Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.
Yonekura
H
H
Unno
M
M
Suzuki
Y
Y
Watanabe
T
T
Moriizumi
S
S
Takasawa
S
S
Okamoto
H
H
D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000378:metabolism; D017930:Genes, Reporter; D051847:Lithostathine; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C497615:Reg1 protein, mouse; C497616:Reg1a protein, rat; D008156:Luciferases