title: Antithyroid effects in vivo and in vitro of babassu and mandioca: a staple food in goiter areas of Brazil.
abstract: Babassu (Orbignya phalerata), a palm-tree coconut fruit, mixed with mandioca (Manihot utilissima) is the staple food of people living in the endemic goiter area of Maranhao in Brazil, where goiter prevalence among schoolchildren was still 38% in 1986 despite an adequate iodine intake in most of the population. Therefore, the question arose as to whether or not the ingestion of babassu alone or mixed with mandioca contributed to the persistence of endemic goiter in this area of Brazil. In this investigation we examined the potential antithyroid effects of babassu and mandioca by means of in vivo studies in Sprague-Dawley rats, in vitro studies in porcine thyroid slices and using a purified porcine thyroid peroxidase (TPO) system. Samples of various edible parts of babassu and mandioca flour were homogenized and extracted in goitrogen-free water (GFW) for in vivo experiments, and in methanol (100 g/l), GFW or 0.06 mol/l phosphate buffer (pH 7.0) for in vitro experiments. The edible parts of babassu produced significant in vivo antithyroid effects (p < 0.05- < 0.001) in rats on a high iodine intake (14 micrograms I- day-1.rat-1), as well as distinct and reproducible antithyroid and anti-TPO activities in both in vitro systems, their action being similar to that of the thionamide-like antithyroid drugs propylthiouracil and methimazole.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: University of Mississippi School of Medicine, Jackson.
authors.forename: E
authors.initials: E
authors.lastname: Gaitan
authors.forename: R C
authors.initials: RC
authors.lastname: Cooksey
authors.forename: J
authors.initials: J
authors.lastname: Legan
authors.forename: R H
authors.initials: RH
authors.lastname: Lindsay
authors.forename: S H
authors.initials: SH
authors.lastname: Ingbar
authors.forename: G
authors.initials: G
authors.lastname: Medeiros-Neto
mesh_terms: D000818:Animals; D001938:Brazil; D005260:Female; D005502:Food; D066298:In Vitro Techniques; D007453:Iodide Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D010945:Plants, Edible; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D013961:Thyroid Gland / Q000187:drug effects / Q000502:physiology*
chemical_list: D010936:Plant Extracts; D007453:Iodide Peroxidase