title: Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR.
abstract: Polymerase chain reaction (PCR) of a TATA-binding protein cDNA that contains CAG triplet repeats results in heterogeneous products. This is caused by a variable loss in the number of CAG triplets. Sequence analysis of PCR products suggests that instability increases with repeat length.
authors.affiliation: Laboratory for Physiological Chemistry, Utrecht University, The Netherlands.
authors.forename: F C
authors.initials: FC
authors.lastname: Holstege
authors.forename: P C
authors.initials: PC
authors.lastname: van der Vliet
authors.forename: H T
authors.initials: HT
authors.lastname: Timmers
mesh_terms: D016477:Artifacts; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors