title: Evaluation of five immunoassays for detection of Chlamydia psittaci in cloacal and conjunctival specimens from turkeys.
abstract: Five commercially available immunoassays were evaluated for the detection of Chlamydia psittaci in cloacal and conjunctival swabs from industrially raised turkeys: IMAGEN (DAKO Diagnostics, Ely, Cambridgeshire, United Kingdom), Chlamydia CEL-VET IF (Cellabs, Brookvale, Australia), IDEIA (DAKO Diagnostics), CELISA (Cellabs), and CLEARVIEW (Unipath, Bedford, United Kingdom). Results were compared with isolation in Buffalo Green Monkey cells as a reference method. For the conjunctival samples, the sensitivities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, the CELISA, and the CLEARVIEW test were found to be 100, 66, 0, 0, and 0%, respectively, as compared to the reference test. Also for the conjunctival samples, the specificities of the IMAGEN test, the Chlamydia CEL-VET IF test, and the IDEIA were found to be 100, 11, and 92.8%, respectively. For the cloacal specimens, the sensitivities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, the CELISA, and the CLEARVIEW test were found to be 100, 93.3, 26.6, 0, and 53.3%, respectively. Also for the cloacal specimens, the specificities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, and the CLEARVIEW test were found to be 92, 12, 100, and 88%, respectively. The IMAGEN test was the most sensitive and specific direct chlamydia antigen detection test for cloacal and conjunctival samples from turkeys.
authors.affiliation: Department of Avian Diseases, State University of Ghent, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Vanrompay
authors.forename: A
authors.initials: A
authors.lastname: Van Nerom
authors.forename: R
authors.initials: R
authors.lastname: Ducatelle
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
mesh_terms: D000818:Animals; D002691:Chlamydophila psittaci / Q000276:immunology / Q000302:isolation & purification*; D002988:Cloaca / Q000382:microbiology*; D003228:Conjunctiva / Q000382:microbiology*; D003234:Conjunctivitis, Bacterial / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D007118:Immunoassay; D011201:Poultry Diseases / Q000382:microbiology*; D009956:Psittacosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014422:Turkeys / Q000382:microbiology*
chemical_list: D011933:Reagent Kits, Diagnostic