title: Purification of the LysR family regulator, ClcR, and its interaction with the Pseudomonas putida clcABD chlorocatechol operon promoter.
abstract: Previous studies have shown that the clcABD operon is under the transcriptional control of the LysR-type activator ClcR. In this study, the conditions leading to its aggregation were avoided and ClcR was purified and confirmed by amino-terminal sequencing. Gel filtration indicated that ClcR exists as a dimer in solution. The DNase I footprint of ClcR was determined. The binding properties of ClcR and the catechol operon regulator, CatR, were compared.
authors.affiliation: Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago 60612.
authors.forename: W M
authors.initials: WM
authors.lastname: Coco
authors.forename: M R
authors.initials: MR
authors.lastname: Parsek
authors.forename: A M
authors.initials: AM
authors.lastname: Chakrabarty
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D002396:Catechols / Q000378:metabolism*; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D003850:Deoxyribonuclease I; D004926:Escherichia coli; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000302:isolation & purification* / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002396:Catechols; C089121:ClcR protein, bacteria; D011994:Recombinant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C031137:3-chlorocatechol; C073470:LysR protein, Bacteria; D003850:Deoxyribonuclease I