Structure and DNA-binding properties of Pax-QNR, a paired box- and homeobox-containing gene.
After differential screening of a complementary DNA library constructed from quail neuroretina cells (QNR) infected with the v-myc-containing avian retrovirus MC29, we have isolated a complementary DNA clone which identifies a mRNA essentially expressed in the neuronal layer of the retina. This complementary DNA encodes a protein containing paired box and homeobox domains. This gene, called Pax-QNR, is homologous to the murine Pax-6 and human AN genes, which are mutated in the autosomal dominant mutation small eye (Sey) of the mouse and aniridia in humans. Here, we report the genomic exon-intron organization, as well as the existence of alternative splicing events taking place at both the 5' end and the middle part of the gene. A Pax-QNR clone translated in reticulocyte lysate directed the synthesis of a 46 kilodalton protein able to bind specifically to the e5 sequence present upstream of the Drosophila even-skipped gene and target of the Drosophila paired protein. The Pax-QNR paired and homeobox domains individually expressed in bacteria are both able to recognize the e5 sequence.
Dozier
C
C
Centre National de la Recherche Scientifique, URA 1160, Institut Pasteur de Lille, France.
Carrière
C
C
Grévin
D
D
Martin
P
P
Quatannens
B
B
Stéhelin
D
D
Saule
S
S
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005091:Exons; D005801:Genes, Homeobox; D016678:Genome; D008969:Molecular Sequence Data; D011784:Quail / Q000196:embryology / Q000235:genetics*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins