[The mechanisms of the antiviral action of the bora-adamantane derivative preparation BG-12].
The effect of one of the derivatives of boraadamantane, preparation BG-12, on reproduction of influenza type A and B viruses was studied. This preparation was shown to inhibit multiplication of a wide range of influenza type A and B virus strains. It is important that BG-12 inhibits in cell culture the replication of a mutant of fowl plague virus A/FPV/Weibridge resistant to remantadine. BG-12 was found to exert a marked viricidal effect, to inhibit the hemolytic activity of both influenza A and influenza B viruses, but not the neuraminidase activity of these viruses. The synthesis of some virus-specific proteins was found to be disturbed in influenza B/Ann Arbor/86-infected cells in the presence of BG-12. A FPVBAR mutant of influenza A/FPV/Weibridge (H7N7) virus resistant to BG-12 was obtained. All the BG-12-resistant reassortants inherited genes 4 and 7 coding for hemagglutinin (HA) and membrane proteins (M) from FPVBAR mutant, respectively, and the remaining genes from BG-12-sensitive A/Krasnodar/101/59 (H2N2) virus. The sequencing of a region of the M gene encoding for the transmembrane protein M2 revealed the substitution of Ala30-Thre in this protein. Multiple attempts at generating a mutant of influenza type B virus resistant to BG-12 failed.
Markushin
S G
SG
Sinitsyn
B V
BV
Ginzburg
V P
VP
Khaĭder
A M
AM
Klimov
A I
AI
D000218:Adamantane / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000998:Antiviral Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D001483:Base Sequence; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D016679:Genome, Viral; D009980:Influenza A virus / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D009981:Influenza B virus / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D016865:Reassortant Viruses / Q000187:drug effects / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D014764:Viral Proteins; D000218:Adamantane
Izuchenie mekhanizmov protivovirusnogo deĭstviia proizvodnogo boraadamantana preparata BG-12.