title: Evaluation of direct disc diffusion susceptibility testing for bacteriuria using diluted urine against standard CDS method.
abstract: Consecutive urine specimens with > or = 10(9) organisms/L on microscopy were diluted 1:100 and direct disc diffusion susceptibility tests performed. Subsequently, the standard Calibrated Dichotomous Sensitivity (CDS) test was performed on all isolates. Urines with > 2 Isolates or where growth was < 10(8) colony forming units (CFU)/L were excluded. Only Gram negative organisms were considered. 361 urines were evaluated, 324 with one and 37 with 2 isolates, comprising 255 Escherichia coli, 49 klebsiella, 41 proteus, 29 either citrobacter, enterobacter, providencia, serratia or alcaligenes, and 14 Pseudomonas aeruginosa. There were 2272 organism/antimicrobial test comparisons. A concordance of 98.5% was obtained. The results are considered acceptable for routine clinical use.
authors.affiliation: Division of Microbiology, Southpath, St George Hospital, Kogarah, NSW.
authors.forename: C
authors.initials: C
authors.lastname: Mukerjee
authors.forename: E
authors.initials: E
authors.lastname: Reiss-Levy
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001437:Bacteriuria / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D005069:Evaluation Studies as Topic; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests; D014556:Urine / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents