title: Molecular epidemiology of adenoviruses isolated in Iceland during 1988-1990.
abstract: All human adenoviruses isolated in Iceland during 1988-1990 have been subjected to restriction endonuclease analysis. Of 55 isolates altogether, subgenus C (Ad1, Ad2, and Ad5) predominated with 42 isolates followed by subgenus B (Ad3 and Ad7) with 12. Analysis of the 9 Ad1 isolates revealed 6 DNA-variants. Among these the established DNA-variants D4, D7, and D10 were recognized. The remaining 3 DNA-variants were primarily found in Iceland. Among the 22 Ad2 isolates, 7 DNA-variants could be distinguished. D2 predominated with 15 isolates whereas the prototype was isolated only once. The novel 5 DNA-variants of Ad2 were all closely related to D2. Analysis of the 11 Ad5 isolates revealed 6 DNA-variants, 2 of which (D2 and D5) were already established. Ice2 and D3 were the most common occurring DNA-variants of Ad5. Ad5 showed the highest degree of genomic variability within subgenus C, both in terms of the low degree of pair-wise comigration of restriction fragments and the number of principal variants of RE-patterns. Analysis of the 9 Ad3 isolates revealed 3 DNA-variants: D3, D10, and Ice1 (a novel DNA-variant that resembles D10). The DNA-variants D3 and D10 were each represented by 4 isolates. The three Ad7 isolates belonged all to the DNA-variant D5.
authors.affiliation: Department of Clinical Microbiology, Karolinska Hospital, Stockholm, Sweden.
authors.forename: M E
authors.initials: ME
authors.lastname: Johansson
authors.forename: M
authors.initials: M
authors.lastname: Ahrel-Andersson
authors.forename: S
authors.initials: S
authors.lastname: Gudnadottir
mesh_terms: D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000235:genetics; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003106:Colon / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D007055:Iceland / Q000453:epidemiology; D010614:Pharynx / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D013997:Time Factors