Antigens of Vibrio vulnificus.
Rabbit antisera raised against a formalinized culture of Vibrio vulnificus were examined for the development of IgM and IgG antibody by ELISA analysis of serum samples harvested weekly for 5 weeks following the initial injection. V. vulnificus antigens recognized by the sera in three fractions of the culture were identified by immunoblot analysis. The major species recognized in the culture supernatant solution by both IgM and IgG had a molar mass of 58 kD. In fractions derived from the bacterial pellet, IgM recognized a species of 56 kD, while IgG reacted strongly with 29.5, 56, 59, 68, 87, and 186 kD species.
Bradley
J L
JL
Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville 32610.
Gaskin
J M
JM
McGuire
P M
PM
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D011817:Rabbits; D013997:Time Factors; D014733:Vibrio / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M