title: Preparation of mouse-human chimeric antibody to an embryonic carbohydrate antigen, Lewis Y.
abstract: Stage-specific embryonic antigen-1 (SSEA-1) is a well-known carbohydrate antigen that is specifically expressed on the surface of cancer cells as well as embryonic cells. In this study, starting with a previously established hybridoma producing a monoclonal antibody (named H18A) to Lewis Y antigen, which is closely related to SSEA-1, we cloned genomic DNA encoding active variable regions both of heavy and light chains of the antibody. Sequence analysis showed that VH and V kappa genes of H18A were in the VH 7183 family and V kappa C1 family, respectively. A transfected cell line named HC-H18A-7 expressing a recombinant chimeric H18A composed of mouse-derived antigen-binding variable regions and human-derived constant regions was established. The chimeric H18A was purified to homogeneity and shown to bind purified Lewis Y antigen with the same dose-response curve as the original H18A. The chimeric H18A looks more promising for clinical application than the original mouse-derived H18A because its antigenicity is expected to be reduced.
authors.affiliation: Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa.
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: Y
authors.initials: Y
authors.lastname: Iba
authors.forename: K
authors.initials: K
authors.lastname: Zenita
authors.forename: K
authors.initials: K
authors.lastname: Shigeta
authors.forename: K
authors.initials: K
authors.lastname: Nakano
authors.forename: W
authors.initials: W
authors.lastname: Itoh
authors.forename: Y
authors.initials: Y
authors.lastname: Kurosawa
authors.forename: R
authors.initials: R
authors.lastname: Kannagi
authors.forename: K
authors.initials: K
authors.lastname: Yasukawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002240:Carbohydrate Sequence; D004247:DNA / Q000737:chemistry; D005803:Genes, Immunoglobulin; D005822:Genetic Vectors; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000737:chemistry / Q000235:genetics; D007983:Lewis Blood Group Antigens / Q000737:chemistry / Q000276:immunology*; D016256:Lewis X Antigen / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014162:Transfection
chemical_list: D000906:Antibodies; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; D011993:Recombinant Fusion Proteins; D004247:DNA