title: Glomerular hemodynamics in cell-free and erythrocyte-perfused isolated rat kidney.
abstract: The cell-free isolated perfused kidney (IPK) is characterized by normal glomerular filtration rate (GFR) and very low filtration fraction (FF). Addition of erythrocytes to the perfusate (IEPK) increases FF while maintaining "normal" GFR levels. Micropuncture studies were performed in IPK and IEPK to establish the determinants of the glomerular ultrafiltration process responsible for low FF in IPK and to evaluate the impact of the addition of erythrocytes on these determinants. Nephron filtration rate was similar in IPK and IEPK (40 +/- 4 vs. 39 +/- 4 nl/min), whereas nephron perfusate flow was significantly higher in IPK (1,247 +/- 100 vs. 112 +/- 13 nl/min), leading to a superficial nephron FF of 3.4 +/- 0.2% in IPK and 36 +/- 2% in IEPK. Glomerular hydrostatic pressure (PG) and transcapillary hydrostatic pressure gradient (delta P) were 53 +/- 2 and 33 +/- 1 mmHg, respectively, in IPK and 51 +/- 3 and 34 +/- 2 mmHg in IEPK, all normal values. Glomerular arteriolar resistances were significantly lower in IPK than in IEPK, and the glomerular ultrafiltration coefficient (LpA) was significantly lower in IPK (0.053 +/- 0.010 vs. 0.100 +/- 0.020 nl.s-1.mmHg-1), but both values are within the normal in vivo range. These results demonstrate that low FF in IPK is not due to decreased delta P or LpA values but to the high renal perfusion rate required to maintain normal PG and delta P values. Addition of erythrocytes increases glomerular arteriolar resistances and restores glomerular hemodynamics to a pattern nearly identical to in vivo conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Nephrology-Hypertension, University of California, San Diego, School of Medicine 92161.
authors.forename: F B
authors.initials: FB
authors.lastname: Gabbai
authors.forename: O W
authors.initials: OW
authors.lastname: Peterson
authors.forename: S
authors.initials: S
authors.lastname: Khang
authors.forename: C B
authors.initials: CB
authors.lastname: Wilson
authors.forename: R C
authors.initials: RC
authors.lastname: Blantz
mesh_terms: D000818:Animals; D002474:Cell-Free System; D004912:Erythrocytes / Q000502:physiology*; D005919:Glomerular Filtration Rate; D006439:Hemodynamics; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D007668:Kidney / Q000502:physiology; D007678:Kidney Glomerulus / Q000502:physiology*; D008297:Male; D009399:Nephrons / Q000098:blood supply / Q000502:physiology; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation