Modified simple cold storage of rat livers with UW solution.
Rat livers were preserved with the conventional use of UW solution for 30, 42, and 48 hr and compared with livers in which the vascular bed was expanded with an additional 10 to 60 ml UW/100 g liver. The extra UW, expressed as % liver weight, was entrapped during final portal infusion by typing off the supra- and infrahepatic inferior vena cava. A beneficial influence of the vascular expansion was most pronounced in the 40% group, with 10/10, 5/10, and 3/10 long-term survivors following transplantation after 30, 42, and 48 hr preservation versus 3/10 and 0/10 after 30 and 42 hr in the 0% controls. In separate experiments, surrogate indices of preservation quality following reperfusion explained this effect. The 40%--and, to a lesser extent, 20%--livers had higher and more uniformly distributed portal blood flow, better tissue oxygenation, smaller increases in postperfusion liver enzymes, higher adenine nucleotides and energy charge, and less histopathologic evidence of hemorrhage and congestion. Pressure changes in the vena cava fluid sump in additional experiments indicated that retrograde infusion of the trapped UW solution occurred in all of the 10-60% groups during the first 6 hr with stable pressures of 1.5 to 3 cm H2O thereafter. Collectively, these data suggest that the much discussed selective vulnerability of the microvasculature of stored allografts is due in part (or principally) to its selective lack of long-term exposure to the UW solution, which drains out of the open vessels but not from the parenchyma. The potential clinical exploitation of this concept is discussed.
Zeng
Q H
QH
Pittsburgh Transplant Institute, University of Pittsburgh Medical Center, Pennsylvania 15213.
Todo
S
S
Murase
N
N
Zhang
S
S
Doria
C
C
Nakamura
K
K
Azzarone
A
A
Starzl
T E
TE
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005978:Glutathione; D006085:Graft Survival / Q000502:physiology; D007262:Infusions, Intravenous; D007328:Insulin; D008102:Liver Circulation / Q000502:physiology; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D019188:Organ Preservation Solutions; D010101:Oxygen Consumption; D011169:Portal Vein / Q000502:physiology; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D014021:Tissue Preservation
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose