Rapid loss of CD4+ T cells in human-PBL-SCID mice by noncytopathic HIV isolates.
Human immunodeficiency virus (HIV) isolates differ in cell tropism, replication, pathogenicity, and syncytial induction in vitro. CD4+ T cells were enumerated in severe combined immunodeficient mice transplanted with human peripheral blood leukocytes (hu-PBL-SCID mice) and infected with HIV isolates with different in vitro cytopathicity. Two noncytopathic, macrophage-tropic strains, HIV-1SF162 and HIV-2UC1, induced extensive CD4+ T cell depletion, whereas HIV-1SF33, which is highly cytopathic for T cells in vitro, caused little CD4+ T cell depletion at equivalent virus burden. In vitro cytopathicity assays therefore do not predict CD4 depletion in the hu-PBL-SCID model.
Mosier
D E
DE
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.
Gulizia
R J
RJ
MacIsaac
P D
PD
Torbett
B E
BE
Levy
J A
JA
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D003588:Cytopathogenic Effect, Viral; D015658:HIV Infections / Q000276:immunology* / Q000382:microbiology; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D015498:HIV-2 / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D007958:Leukocyte Count; D008231:Lymphopenia / Q000276:immunology*; D051379:Mice; D016513:Mice, SCID; D016133:Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000382:microbiology; D014779:Virus Replication