Identification of trisomy in Macaca fascicularis by fluorescence in situ hybridization with a human chromosome 13 DNA library.
A juvenile macaque monkey with abnormal phenotypic and behavioral features was studied cytogenetically. An additional autosome was found in over 90% of the animal's cultured cells. This chromosome, subsequently identified as number 16 in the macaque karyotype by G-banding, was shown to be mostly homologous with human chromosome 13 using fluorescence in situ hybridization of a human chromosome specific cosmid library. Although the monkey, now deceased, exhibited some abnormal physical and behavioral features, none of the severe clinical characteristics associated with human chromosome 13 trisomy were apparent. We suggest that the incomplete expression of 13-trisomy observed could result if the macaque chromosome were deficient in some of the region(s) of chromosome 13 common to humans affected with the disorder.
Ward
O G
OG
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson 85721.
Miller
R L
RL
Johnson
E H
EH
Lucas
J N
JN
Meyne
J
J
D000818:Animals; D002478:Cells, Cultured; D002871:Chromosome Banding; D002882:Chromosomes, Human, Pair 13; D002885:Chromosomes, Human, Pair 16; D004247:DNA / Q000032:analysis*; D004314:Down Syndrome / Q000235:genetics; D005260:Female; D015723:Gene Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000473:pathology; D008252:Macaca fascicularis; D008297:Male; D008992:Monkey Diseases / Q000235:genetics*; D014314:Trisomy
D004247:DNA