Respiration of [14C]alanine by the ectomycorrhizal fungus Paxillus involutus.
The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [14C]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [14C]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed 14CO2 evolution and greatly reduced the flow of carbon from [14C]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.
Chalot
M
M
Department of Microbial Ecology, Lund University, Sweden.
Brun
A
A
Finlay
R D
RD
Söderström
B
B
D000363:Agaricales / Q000378:metabolism*; D000409:Alanine / Q000378:metabolism*; D010101:Oxygen Consumption; D013559:Symbiosis
D000409:Alanine