IL-5 mRNA expression in blood lymphocytes from patients with Kimura's disease and parasite infection.
Blood lymphocytes from patients with eosinophilia are known to produce interleukin-5 (IL-5) with appropriate stimulation in vitro. To determine whether blood lymphocytes from these patients produce IL-5 in vivo, we tested the IL-5 mRNA expression in blood lymphocytes immediately after separation by reverse-transcriptase polymerase chain reaction (RT-PCR) method. We found that lymphocytes from eosinophilic patients expressed IL-5 mRNA, but lymphocytes from normal volunteers did not express the lymphokine. These findings suggest that in patients with eosinophilia, peripheral blood lymphocytes produce IL-5 in vivo.
Enokihara
H
H
Third Department of Internal Medicine, Dokkyo University, School of Medicine, Tochigi, Japan.
Koike
T
T
Arimura
H
H
Aoyagi
M
M
Watanabe
K
K
Nakamura
Y
Y
Yamashiro
K
K
Tsuruoka
N
N
Tsujimoto
M
M
Saito
K
K
D000328:Adult; D000796:Angiolymphoid Hyperplasia with Eosinophilia / Q000097:blood* / Q000150:complications* / Q000235:genetics; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D001483:Base Sequence; D002478:Cells, Cultured; D002648:Child; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015848:Interleukin-5 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D008214:Lymphocytes / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010237:Paragonimiasis / Q000097:blood* / Q000150:complications* / Q000276:immunology; D010238:Paragonimus / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics
D000909:Antibodies, Helminth; D017077:Culture Media, Conditioned; D015848:Interleukin-5; D012333:RNA, Messenger