Cross-reactions of human lupus autoantibodies with 8-methoxypsoralen photomodified DNA fragments.
DNA fragments of around 200 base pair (average size) have been covalently crosslinked with 8-methoxypsoralen under 365 nm UV light. The photoadduct, induced antibodies in rabbits with a titer of > 1:12,800 by direct binding ELISA. Binding data showed that the induced antibodies are conformation-specific recognizing restricted conformational change at site of crosslinking. Human autoantibodies against DNA, bound not only to native DNA but to the photomodified DNA fragment as well. In addition, binding patterns of SLE sera obtained from different patients were remarkably similar, indicating the recognition of altered conformation of the modified polymer by naturally occurring SLE anti-DNA autoantibodies.
Arjumand
S
S
Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.
Ali
A
A
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D003429:Cross Reactions; D003432:Cross-Linking Reagents; D004247:DNA / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008730:Methoxsalen / Q000378:metabolism*; D011817:Rabbits; D014466:Ultraviolet Rays
D001323:Autoantibodies; D003432:Cross-Linking Reagents; D004247:DNA; D008730:Methoxsalen