Determination of vanadium in clam tissue (Citterea sp.) by normal-phase high-performance liquid chromatography with N-phenylbenzohydroxamic acid.
A normal-phase high-performance liquid chromatography (HPLC) method for the selective determination of vanadium with N-phenylbenzohydroxamic acid (PBHA) is described. The Vv-PBHA complex was preconcentrated by solvent extraction into trichloromethane and injected on to a silica gel column for HPLC, with a mobile phase of methanol-trichloromethane (3 + 97) containing 9.4 x 10(-4) mol dm-3 PBHA. The detection limit for vanadium by the proposed method was 8.3 and 1.7 micrograms dm-3 for phase-volume ratios of 5:1 and 20:1 (aqueous-to-organic), respectively. Vanadium has been determined in clam tissue with satisfactory precision.
Bagur
G
G
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Spain.
Sánchez
M
M
Gázquez
D
D
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006877:Hydroxamic Acids; D014639:Vanadium / Q000032:analysis*
D006877:Hydroxamic Acids; D014639:Vanadium