Real-time monitoring of endogenous noradrenaline release in rat brain slices using fast cyclic voltammetry. 2. Operational characteristics of the alpha 2 autoreceptor in the bed nucleus of stria terminalis, pars ventralis.
Fast cyclic voltammetry (FCV) at carbon fibre microelectrodes was used to monitor stimulated noradrenaline (NA) efflux in slices of the ventral part of the rat bed nucleus of stria terminalis (BSTV) superfused with artificial cerebrospinal fluid at 32 degrees C. NA efflux was evoked by local electrical stimulation (trains of 10-50 pulses, 0.2 ms duration, 10 mA constant current at 10-500 Hz). The effects of four alpha 2 antagonists (yohimbine, rauwolscine, prazosin and WB 4101) and three alpha 2 agonists (clonidine, oxymetazoline and UK 14304) were examined. All drugs (1 microM) were added via the superfusate. Yohimbine and rauwolscine increased NA efflux on the lower but not the higher frequency trains: maximum increases (on 20 Hz, 50 pulse stimulation) were to 392 +/- 63% (yohimbine) and 243 +/- 7% (rauwolscine). There was a threshold train duration for demonstration of autoreceptor antagonism of 500-1000 ms. Prazosin and WB 4101 did not increase NA efflux but caused a modest decrease at the higher (100-500 Hz) frequencies. The effects of the alpha 2 agonists were also affected by stimulus train duration. Longer trains reduced agonist (clonidine) effects. When tested on pseudo-one pulse (POP) stimulations (less than 100 ms duration), the alpha 2 agonists decreased NA efflux. UK 14304 reduced NA efflux on 20 pulse/200 Hz stimulation to a greater degree (86 +/- 7%) than the partial agonists clonidine (39 +/- 3%) or oxymetazoline (40 +/- 8%). The present results demonstrate that alpha 2 autoreceptors are a major mechanism in the control of NA efflux in the BSTV.(ABSTRACT TRUNCATED AT 250 WORDS)
Palij
P
P
Anaesthetics Unit, London Hospital Medical College, Royal London Hospital, Whitechapel, UK.
Stamford
J A
JA
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D002552:Cerebral Ventricles / Q000378:metabolism*; D004558:Electric Stimulation; D004566:Electrodes; D004594:Electrophysiology; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*
D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D011942:Receptors, Adrenergic, alpha; D009638:Norepinephrine