Luminol-enhanced chemiluminescence by Listeria monocytogenes: a test for rapid assessment of antimicrobial agents.
Listeria monocytogenes produces chemiluminescence in brain heart infusion broth at 37 degrees C in the presence of carbonate ions and acetaldehyde. This phenomenon can be enhanced by the use of luminol rather than acetaldehyde. Furthermore, there is direct relationship between the extent of growth and the level of luminescence which culminates at the end of the exponential growth. This property was used to study the susceptibility of this bacterium to two antiseptics, cetrimonium bromide and chlorhexidine, and to two antibiotics, ampicillin and chloramphenicol. Inhibition of chemiluminescence was proportional to the antimicrobial agents' concentrations and was complete at their minimal inhibitory concentrations.
Vidon
D J
DJ
Département des Sciences de l'Aliment, Université Louis Pasteur, Illkirch, France.
André
P
P
Baaj
D
D
Gryczka
C
C
Philipp
P
P
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; D008165:Luminol