title: Mutation of the p53 gene in gallbladder cancer.
abstract: Genetic alteration of the p53 gene was examined in tissue specimens of primary gallbladder cancer (GBC) using PCR-single strand conformation polymorphism (SSCP) and direct sequencing analyses. The p53 gene mutation was detected in five of the 16 GBC cases examined (31%). All five cases showed single point mutations; four were mutations resulting in amino acid substitution and the other was one base-pair deletion. Of the five mutations detected, four occurred at evolutionarily conserved regions and the remaining one at a well conserved region among species. Our result is similar to those of previous reports for various human cancers in mutation scattering over four of the five conserved domains.
authors.affiliation: Department of Hygiene and Preventive Medicine, Niigata University School of Medicine.
authors.forename: S
authors.initials: S
authors.lastname: Takagi
authors.forename: E
authors.initials: E
authors.lastname: Naito
authors.forename: H
authors.initials: H
authors.lastname: Yamanouchi
authors.forename: H
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: R
authors.initials: R
authors.lastname: Kominami
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D004252:DNA Mutational Analysis; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005706:Gallbladder Neoplasms / Q000209:etiology / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm