title: Humoral and cellular immune responses in experimental trichinosis.
abstract: This work was designed to study the relation between cellular and humoral immune responses in experimentally infected albino rats with T. spiralis larvae over a period of four weeks. Cell-mediated immune response (measured by lymphoblast transformation test) revealed a marked lymphoblast response during the intestinal phase, but the reaction became irregular with larval encystation in muscles. Humoral immune response was estimated by both of micro-and Dot-ELISA techniques. It was obvious that significant higher O.D. readings and dot color intensities were observed in the muscular phase than in intestinal phase. The results proved that micro-ELISA had the least accuracy (66.6%) and poorest specificity (81.25%), while Dot-ELISA showed the best sensitivity (100%) and highest specificity (87.5%). Lymphoblast transformation was as specific as Dot-ELISA.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Zagazig University, Egypt.
authors.forename: M M
authors.initials: MM
authors.lastname: abou Hassan
authors.forename: M A
authors.initials: MA
authors.lastname: Seleem
authors.forename: S E
authors.initials: SE
authors.lastname: Etewa
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D051381:Rats; D012680:Sensitivity and Specificity; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth