title: Swaledale sheep affected by natural scrapie differ significantly in PrP genotype frequencies from healthy sheep and those selected for reduced incidence of scrapie.
abstract: PrP glycoprotein gene polymorphisms were examined in Swaledale sheep affected by natural scrapie, in healthy sheep and in Swaledales selected for low susceptibility to scrapie. The three groups differed significantly in frequencies of PrP genotypes detected by the restriction enzymes EcoRI, HindIII and BspHI, the latter being indicative of a PrP protein amino acid difference at codon 136. These frequency differences were confirmed in a single-flock study and present good evidence that scrapie susceptibility and resistance are associated with PrP gene variants in Swaledale sheep.
authors.affiliation: Institute for Animal Health, AFRC/MRC Neuropathogenesis Unit, Edinburgh, U.K.
authors.forename: N
authors.initials: N
authors.lastname: Hunter
authors.forename: W
authors.initials: W
authors.lastname: Goldmann
authors.forename: G
authors.initials: G
authors.lastname: Benson
authors.forename: J D
authors.initials: JD
authors.lastname: Foster
authors.forename: J
authors.initials: J
authors.lastname: Hope
mesh_terms: D000367:Age Factors; D000818:Animals; D015247:Deoxyribonuclease HindIII; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics; D015994:Incidence; D011110:Polymorphism, Genetic; D018620:PrPSc Proteins; D011328:Prions / Q000235:genetics*; D015183:Restriction Mapping; D012608:Scrapie / Q000235:genetics*; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000235:genetics*; D013045:Species Specificity
chemical_list: D018620:PrPSc Proteins; D011328:Prions; D015247:Deoxyribonuclease HindIII