Use of the insertion element IS6110 for DNA fingerprinting of Mycobacterium tuberculosis isolates presenting various profiles of drug susceptibility.
IS6100 is an insertion sequence of the IS3 family and it is present in multiple copies in the chromosome of Mycobacterium tuberculosis. Four to 15 copies are present in various strains of M. tuberculosis. In this study, the value of IS6110 as an epidemiological marker of tuberculosis was examined. Unrelated clinical strains from Greek patients presented, in restriction fragment length polymorphism analysis, a high degree of polymorphism, whereas patterns of related clinical strains from familial outbreaks were identical. Since RFLP analysis with acetylaminofluorene labeled IS6110 as the probe gave satisfactory results, it is suggested that this non-radioactive probe can be used in hospitals and health centres for the epidemiological survey of M. tuberculosis infections.
Thierry
D
D
Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, Paris, France.
Matsiota-Bernard
P
P
Pitsouni
E
E
Costopoulos
C
C
Guesdon
J L
JL
D015073:2-Acetylaminofluorene; D001483:Base Sequence; D016172:DNA Fingerprinting; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D015073:2-Acetylaminofluorene