Protective effect of antioxidants against free radical-mediated lipid peroxidation induced by DON or T-2 toxin.
An experiment was conducted on rats to investigate the capacity of antioxidants to protect against acute toxicity caused by DON or T-2 toxin. Male rats were fed two different feeds. One group received a feed deficient in vitamins C and E and selenium, whereas the other group was fed with a feed enriched in antioxidants. After two weeks, selected groups of rats were administered orally a single dose of DON or T-2 toxin. After the treatment with mycotoxins, all rats were decapitated. The livers were analyzed for TBARS values, hepatic GSH content and for the activities of CyP-450, CAT, SOD and GSH-TR. Increases in lipid peroxides of 21% and 268% were observed in those rats which did not receive the supplement of antioxidants and which were administered DON or T-2 toxin, respectively. There was no significant increases in the TBARS values in the groups receiving DON with selenium and vitamins, but increases of 57% and 79% were recorded in the groups administered T-2 toxin and antioxidants. Furthermore, in the groups fed the deficient feed and administered DON or T-2 toxin, the lipid peroxidation increased by 33% and 307%, respectively. No mortality, and a lower number of intoxicated animals were observed in rats fed a diet supplemented with antioxidants. Significant decreases of GSH, CAT, SOD, CyP-450 and GSH-TR were recorded in treated rats receiving the deficient feed. The results of this study demonstrate that trichothecenes stimulate lipid peroxidation with consequent decrease of GSH content, but that the dietary use of selenium, alpha-tocopherol and ascorbic acid provides protection against acute toxicosis caused by DON or T-2 toxin.
Rizzo
A F
AF
National Veterinary and Food Research Institute, Department of Chemistry, Helsinki, Finland.
Atroshi
F
F
Ahotupa
M
M
Sankari
S
S
Elovaara
E
E
D000818:Animals; D001205:Ascorbic Acid / Q000627:therapeutic use*; D005609:Free Radicals; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology; D013605:T-2 Toxin / Q000633:toxicity*; D014255:Trichothecenes / Q000633:toxicity*; D014810:Vitamin E / Q000627:therapeutic use*
D005609:Free Radicals; D014255:Trichothecenes; D014810:Vitamin E; D012643:Selenium; D013605:T-2 Toxin; C007262:deoxynivalenol; D001205:Ascorbic Acid