Effects of extended low-dose-rate exposure to 137Cs detected by flow-cytometric enumeration of micronucleated erythrocytes in mouse peripheral blood.
An automated variant the of micronucleus assay for erythrocytes in mouse peripheral blood has recently been developed. The flow-cytometric technique used allows very large numbers of erythrocytes to be analysed with a relatively small effort. Here we report the potential of this method to detect a response to extended low-dose-rate exposure to gamma-irradiation. The mice were irradiated with a 137Cs source at a dose rate of 4.8 cGy/day for 26 days. Sampling was continued for another 39 days after irradiation. Elevated frequencies compared with the control group were found at days 2, 9 and 20 after the start of the irradiation for micronucleated polychromatic erythrocytes, and at days 9, 20, 29, 42, 51 and 65 for micronucleated normochromatic erythrocytes.
Grawé
J
J
Department of Pathology, Swedish University of Agricultural Sciences, Uppsala.
Zetterberg
G
G
Amnéus
H
H
D000818:Animals; D002588:Cesium Radioisotopes; D004912:Erythrocytes / Q000528:radiation effects*; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D015162:Micronucleus Tests; D011829:Radiation Dosage; D011831:Radiation Genetics; D011833:Radiation Injuries, Experimental / Q000235:genetics*; D013997:Time Factors
D002588:Cesium Radioisotopes