title: Detection of multiple virulence-associated genes of Listeria monocytogenes by PCR in artificially contaminated milk samples.
abstract: The inhibitory effect of milk in the PCR detection of Listeria monocytogenes could be overcome by washing the contaminated milk sample with phosphate-buffered saline and concentrating the bacteria to 1/10 of the original volume. In order to avoid a possible failure in the detection of virulent L. monocytogenes, a one-step procedure which enabled demonstration of three virulence-associated genes, prfA, hlyA, and plcB, simultaneously in a single PCR mixture was developed.
authors.affiliation: Department of Bacteriology, Niigata University School of Medicine, Japan.
authors.forename: K J
authors.initials: KJ
authors.lastname: Cooray
authors.forename: T
authors.initials: T
authors.lastname: Nishibori
authors.forename: H
authors.initials: H
authors.lastname: Xiong
authors.forename: T
authors.initials: T
authors.lastname: Matsuyama
authors.forename: M
authors.initials: M
authors.lastname: Fujita
authors.forename: M
authors.initials: M
authors.lastname: Mitsuyama
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D029968:Escherichia coli Proteins; D005516:Food Microbiology; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D010454:Peptide Termination Factors; D016133:Polymerase Chain Reaction / Q000379:methods*; D015534:Trans-Activators; D010738:Type C Phospholipases / Q000235:genetics; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D010454:Peptide Termination Factors; D015534:Trans-Activators; D010738:Type C Phospholipases