Macrophage mediated tumor cell destruction measured by an alkaline phosphatase assay.
A new assay to measure the cytotoxic or growth-inhibitory activity of macrophages against tumor cells is described. The method is based on the fact that, in contrast to macrophages, natural killer cells or cytotoxic lymphocytes, a variety of tumor cells have a very high content of alkaline phosphatase. The strong linearity between tumor cell number and alkaline phosphatase activity in the cultures permits evaluation of macrophage function with standard ELISA equipment.
Modolell
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M
Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Munder
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PG
D000469:Alkaline Phosphatase; D000818:Animals; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured / Q000276:immunology*
D000469:Alkaline Phosphatase