Effects of sulfonylurea agents on platelet arachidonic acid metabolism; study on platelet homogenates.
Effects of three sulfonylurea agents on arachidonic acid metabolism of platelet homogenates were evaluated using HPLC. Gliclazide had no significant inhibitory effects on arachidonic acid metabolism. Glibenclamide and glimepiride both inhibited the production of cyclooxygenase-related metabolites, thromboxane B2 (TXB2) and 12-hydroxy 5,8,10-heptadecatrienoic acid (HHT), whereas 12-hydroxy 5,8,10,14-eicosatetraenoic acid (12-HETE), a 12-lipoxygenase-related product, was unaffected. These findings confirmed part of our previous report using intact platelets, except that we found in the present study that glibenclamide had no inhibitory effect on 12-lipoxygenase.
Satoh
K
K
Department of Clinical and Laboratory Medicine, Yamamanasi Medical College, Japan.
Ozaki
Y
Y
Yatomi
Y
Y
Kume
S
S
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D016718:Arachidonic Acid / Q000378:metabolism*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D005907:Gliclazide / Q000494:pharmacology; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D066298:In Vitro Techniques; D013453:Sulfonylurea Compounds / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000096:biosynthesis
D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D013453:Sulfonylurea Compounds; D016718:Arachidonic Acid; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; D013929:Thromboxane B2; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D005907:Gliclazide