title: Macrophages and endothelial cells express intercellular adhesion molecule-1 in immune-mediated demyelination but not in Wallerian degeneration of the rat peripheral nervous system.
abstract: BACKGROUND
  Intercellular adhesion molecule-1 (ICAM-1) plays an important role in immune responses, especially in T cell/endothelial cell and T cell/macrophage interactions. This study reports the cellular localization of ICAM-1 during immune-mediated demyelination of the peripheral nervous system induced by adoptive transfer of P2 specific T cells.
  
  
  METHODS
  Cryosections 1 micron thick of ventral roots of rats with experimental autoimmune neuritis were labeled with a monoclonal antibody against rat ICAM-1.
  
  
  RESULTS
  Numerous ICAM-1 positive cells were present before and shortly after the onset of clinical disease from days 4 to 6 after cell transfer. By day 8, their number had greatly decreased. ICAM-1 positive infiltrating cells could be identified as ED1 positive macrophages. Moreover, endothelial cells expressed ICAM-1. Schwann cells and T cells were ICAM-1 negative. While at early stages of experimental autoimmune neuritis, ICAM-1 and Ia colocalized on macrophages but not endothelial cells, Ia persisted for a longer period in nerve roots than ICAM-1. After nerve transection, macrophages entering the distal stump and endothelial cells did not express ICAM-1.
  
  
  CONCLUSIONS
  The presence of ICAM-1 immunoreactivity in peripheral nerve indicates an underlying immune-mediated process. Among other cytokines interferon-gamma, which is transiently expressed in nerves during immune-mediated demyelination but not after nerve transection, could be one mediator that induces ICAM-1 in immune-mediated demyelination of the peripheral nervous system.
authors.affiliation: Department of Neurology, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Stoll
authors.forename: S
authors.initials: S
authors.lastname: Jander
authors.forename: S
authors.initials: S
authors.lastname: Jung
authors.forename: J
authors.initials: J
authors.lastname: Archelos
authors.forename: T
authors.initials: T
authors.lastname: Tamatani
authors.forename: M
authors.initials: M
authors.lastname: Miyasaka
authors.forename: K V
authors.initials: KV
authors.lastname: Toyka
authors.forename: H P
authors.initials: HP
authors.lastname: Hartung
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism*; D003711:Demyelinating Diseases / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D004727:Endothelium / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D018799:Intercellular Adhesion Molecule-1; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D010523:Peripheral Nervous System Diseases / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes; D014855:Wallerian Degeneration / Q000276:immunology
chemical_list: D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1