Effect of serotonin on protein phosphorylation in the central nervous system of the leech Hirudo medicinalis.
1. Phosphoproteins of different regions of the Hirudo medicinalis central nervous system have been analysed by means of two-dimensional electrophoresis. 2. Serotonin, 8-Br-cAMP and phorbol 12,13-dibutyrate stimulate phosphorylation of a number of proteins whose isoelectric points and molecular weights are presented. 3. A group of proteins of 78 kDa and pI = 6-6.5, whose level of phosphorylation increases in the presence of serotonin, 8-Br-cAMP and phorbol ester, is observed only in segmental but not in cephalic or caudal ganglia. 4. The putative roles of these phosphoproteins are discussed.
Garcia-Gil
M
M
Department of Physiology and Biochemistry, University of Pisa, Italy.
Bottai
D
D
Zaccardi
M L
ML
Cannizzaro
M
M
Brunelli
M
M
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002490:Central Nervous System / Q000187:drug effects* / Q000378:metabolism; D005724:Ganglia / Q000187:drug effects / Q000378:metabolism; D007865:Leeches / Q000187:drug effects* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010750:Phosphoproteins / Q000032:analysis; D010766:Phosphorylation; D012701:Serotonin / Q000494:pharmacology*
D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D012701:Serotonin; D015240:Phorbol 12,13-Dibutyrate