Evaluation of the new Cobas Core IgE Total EIA in comparison to three commercial IgE immunoassays.
Determination of total IgE by the new Cobas Core IgE Total EIA was compared with three other routine methods (Pasteur IgE ELISA, Pasteur IgE RIA and Pharmacia IgE Ultra-PRIST). Mean IgE concentrations in adult sera correlated well among the three methods (Cobas Core IgE Total EIA, Pasteur IgE ELISA and Pasteur IgE RIA); however, the values for the Pasteur IgE RIA were on average 20-50 IU/ml higher than those measured by the enzyme immunoassays. The correlation between the Cobas Core IgE Total EIA and the Pharmacia IgE Ultra-PRIST in sera from newborns was also excellent. The analytical performance of the Cobas Core IgE Total EIA in terms of dilution linearity, recovery and intra-assay reproducibility (coefficient of variation = 1.8-3.8%) was excellent.
de Weck
A L
AL
Institute of Immunology, Inselspital, Bern, Switzerland.
Schluchter
M
M
Girardin
P
P
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood*; D007231:Infant, Newborn; D011863:Radioimmunoassay
D007073:Immunoglobulin E