Cloning and characterization of OSF-3, a new member of the MER5 family, expressed in mouse osteoblastic cells.
A cDNA library prepared from the mouse osteoblastic cell line, MC3T3-E1, was screened for the presence of specifically expressed genes by employing subtraction hybridization/differential screening methods. A cDNA clone was identified and sequenced, encoding a protein designated as osteoblast specific factor 3 (OSF-3) comprising 199 amino acids. RNA dot blot analysis indicated weak OSF-3 expression in thymus, spleen, brain, lung, testis, and heart, and high expression in kidney and liver. A homology search of an amino acid sequence database revealed a strong relationship of OSF-3 to the MER5 (gene preferentially expression in murine erythroleukemia cells) protein and human pag (proliferation associated gene) product. This indicates that OSF-3 plays an intrinsic role in the proliferation and/or differentiation of bone cells.
Kawai
S
S
Pharma Research Laboratories, Hoechst Japan Limited, Saitama.
Takeshita
S
S
Okazaki
M
M
Kikuno
R
R
Kudo
A
A
Amann
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D010006:Osteoblasts / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D011506:Proteins; C089432:osteoblast specific factor 3