In vitro studies on the effects of dehydroepiandrosterone and corticosterone on hepatic steroid receptor binding and mitochondrial respiration.
1. The in vitro effects of dehydroepiandrosterone (DHEA), DHEA-sulfate (DHEA-S) and related steroids on glucocorticoid corticosterone (GC) binding in hepatic cytosol and hepatic mitochondrial respiration were examined in male BHE/cdb and Sprague-Dawley (SD) rats. 2. The Kd and the IC50 for GC binding in SD rats were 5- and 2-fold higher, respectively, than in BHE/cdb rats. 3. Hepatic cytosol from BHE/cdb rats bound six times more [3H]-GC to receptors than that from SD rats. 4. The percentage displacement and Bmax of GC for its receptors were similar for the two strains of rats. 5. DHEA and DHEA-S did not displace GC from its receptors. 6. DHEA and related non-sulfated steroids decreased state 3 mitochondrial respiration, respiratory control and oxidative phosphorylation capacity in a dose-dependent manner with malate + pyruvate as substrate.
McIntosh
M K
MK
Department of Food, Nutrition, University of North Carolina, Greensboro 27412.
Pan
J S
JS
Berdanier
C D
CD
D000818:Animals; D003345:Corticosterone / Q000493:pharmacokinetics / Q000494:pharmacology*; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D011987:Receptors, Steroid / Q000187:drug effects*; D013045:Species Specificity
D011987:Receptors, Steroid; D003687:Dehydroepiandrosterone; D003345:Corticosterone