Use of synthetic peptides to map regions of rubella virus capsid protein recognized by human T lymphocytes.
Synthetic peptides (SPs), 18-29 amino acids long, representing selected sequences of rubella virus (RV) capsid (C) protein were used in lymphocyte proliferation assays to identify antigenic regions recognized by T lymphocytes from healthy RV-reactive adults. Four SPs, C(1-29), C(90-114), C(108-134) and C(255-300), stimulated proliferation of peripheral blood mononuclear cells and RV-specific T-cell lines from the same donors. C(1-29V), an SP analogue containing an RA27/3 RV vaccine strain sequence, stimulated higher levels of proliferation in T cells obtained from RV-vaccinated subjects than did the comparable wild-type (M33 strain) RV sequence.
Mitchell
L A
LA
Department of Pathology, University of British Columbia, Vancouver, Canada.
Décarie
D
D
Tingle
A J
AJ
Lacroix
M
M
Zrein
M
M
D000328:Adult; D000595:Amino Acid Sequence; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000097:blood*; D002213:Capsid / Q000737:chemistry* / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006683:HLA-DQ Antigens / Q000097:blood; D006684:HLA-DR Antigens / Q000097:blood; D006801:Humans; D016130:Immunophenotyping; D008875:Middle Aged; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D012412:Rubella virus / Q000737:chemistry* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000956:Antigens, Viral; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens