RNA transcript mapping of the Wiseana iridescent virus genome.
The replication of Wiseana iridescent virus (WIV) was studied in Lymantria dispar tissue culture cells. Using a combination of [35S]methionine pulse-labeling and Northern blotting with WIV DNA probes, a transcriptional map of the genome was constructed. WIV has a wide dispersal of immediate-early genes with seven different regions identified. WIV has been reported to have extensive repetitive DNA sequences but no early transcription was observed in these regions. Although fine-mapping is required, some early regions (Bam L and Eco O) have been identified which are transcriptionally active at 6- and 12-h but are shut down by 24 h. These regions could provide probes for early genes and the hypothesized switch from nuclear to cytoplasmic replication for iridoviruses.
McMillan
N A
NA
Microbiology Department, University of Otago, Dunedin, New Zealand.
Kalmakoff
J
J
D000818:Animals; D002460:Cell Line; D015342:DNA Probes; D016679:Genome, Viral; D007313:Insecta; D017994:Iridovirus / Q000235:genetics* / Q000502:physiology; D012367:RNA, Viral / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014779:Virus Replication / Q000235:genetics*
D015342:DNA Probes; D012367:RNA, Viral; D014764:Viral Proteins