Structure and expression of the promoter for the human type II transforming growth factor-beta receptor.
The type II TGF-beta receptor is a serine/threonine kinase whose expression is essential for the action of TGF-beta. In this paper, we describe the cloning and expression of the human type II TGF-beta promoter. DNA sequence analysis indicates that the region near the transcription initiation site lacks a TATA and CAAT box but contains Sp1 binding sites. A similar promoter type has been identified in the 5' flanking regions of genes that code for certain other growth factor receptors. Transfection of the promoter (888 bp fragment) mediated transcriptional activity in bovine vascular smooth muscle cells and human lung fibroblasts.
Humphries
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DE
Pulmonary Center at Boston University School of Medicine, Massachusetts.
Bloom
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BB
Fine
A
A
Goldstein
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RH
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014162:Transfection
D018125:Receptors, Transforming Growth Factor beta; D016329:Sp1 Transcription Factor; D004247:DNA; D017346:Protein Serine-Threonine Kinases