title: Energy metabolism in isolated chick (Gallus domesticus) gastrocnemius and tilapia (Tilapia mossambica) epaxial muscle at various temperatures in vitro.
abstract: Muscle respiration experiments on inhibitor dosage (experiment 1), muscle preparation (tendons removed vs. unstretched vs. stretched muscles; chick muscle only; experiment 2) and media temperature (26.5, 32, 37, 42 degrees C; experiment 3) were conducted on chick (Gallus domesticus) gastrocnemius and tilapia (Tilapia mossambica) epaxial muscle in vitro. Experiment 1: The dosage of cycloheximide and ouabain required for maximum inhibition of protein synthesis and Na+,K+ ATPase, respectively, in chick and tilapia muscle was approximately 6 x 10(-5) M. Experiment 2: Removing the tendons of chick muscle decreased (% inhibition, P = 0.05) cycloheximide-sensitive respiration compared to stretched and unstretched muscles (tendons intact). However, muscle preparation had little influence on ouabain-sensitive respiration. Experiment 3: Cycloheximide-sensitive respiration tended to increase (microliter O2/mg DNA.hr, P = 0.054) with media temperature in tilapia muscle. Chick muscle was less responsive in this respect. Ouabain-sensitive respiration increased at lower temperature in chick muscle (% inhibition, cubic relationship, P = 0.001) and at higher temperature in tilapia muscle (% inhibition, quadratic relationship, P = 0.0002).
authors.affiliation: Department of Animal Science, University of Hawaii at Manoa, Honolulu 96822.
authors.forename: E Y
authors.initials: EY
authors.lastname: Suzuki
authors.forename: R J
authors.initials: RJ
authors.lastname: Early
authors.forename: P H
authors.initials: PH
authors.lastname: Patterson
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002645:Chickens / Q000378:metabolism*; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism; D009132:Muscles / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D011506:Proteins / Q000037:antagonists & inhibitors; D013696:Temperature; D017210:Tilapia / Q000378:metabolism*
chemical_list: D011506:Proteins; D010042:Ouabain; D003513:Cycloheximide