Structural comparison and chromosomal localization of the human and mouse IL-13 genes.
The genomic structure of the recently described cytokine IL-13 has been determined for both human and mouse genes. The nucleotide sequence of a 4.6-kb DNA segment of the human gene is described. The human IL-13 gene (IL13) occurs as a single copy in the haploid genome and maps to human chromosome 5. A 4.3-kb DNA fragment of the mouse IL-13 gene (IL13) has been sequenced and found to occur as a single copy, mapping to mouse chromosome 11. Intrachromosomal mapping studies revealed that both genes contain four exons and three introns and show a high degree of sequence identity throughout their length. Potential recognition sequences for transcription factors that are present in the 5'-flanking region and are conserved between both genes include IFN-responsive elements, binding sites for AP-1, AP-2 and AP-3, and NF-IL 6 site, and a TATA-like sequence. Both genes map to chromosomal locations adjacent to genes encoding other cytokines, including IL-3, GM-CSF, IL-5, and IL-4, suggesting that IL-13 is another member of this cytokine gene family that may have arisen by gene duplication.
McKenzie
A N
AN
Department of Molecular Biology, DNAX Research Institute of Cellular and Molecular Biology, Palo Alto, CA 94304.
Li
X
X
Largaespada
D A
DA
Sato
A
A
Kaneda
A
A
Zurawski
S M
SM
Doyle
E L
EL
Milatovich
A
A
Francke
U
U
Copeland
N G
NG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D018793:Interleukin-13; D015847:Interleukin-4 / Q000737:chemistry / Q000235:genetics; D007378:Interleukins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D018793:Interleukin-13; D007378:Interleukins; D015847:Interleukin-4