title: Endothelin increased [Ca2+]i in cultured neurones and slices of rat hippocampus.
abstract: We reported here the first evidence that endothelin (ET)-1 and ET-3 produced an increase in intracellular Ca2+ concentrations ([Ca2+]1), consisting of a transient and a sustained component, in cultured neurones and in slices of the rat hippocampus. In the neurones, the removal of Ca2+ or the addition of Cd2+ remarkably inhibited the ET-3-evoked sustained but not the transient response. The [Ca2+]i increased by ETs was observed in the dentate gyrus and in the vicinity of CA1 and CA3 pyramidal cell layer in the hippocampus, findings in good accord with regions where the glutamate receptor agonists have an elevated [Ca2+]i. The possible causal link between ETs and the increased [Ca2+]i in the development of hippocampal neuronal death is discussed.
authors.affiliation: Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Koizumi
authors.forename: Y
authors.initials: Y
authors.lastname: Kataoka
authors.forename: M
authors.initials: M
authors.lastname: Niwa
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Taniyama
authors.forename: Y
authors.initials: Y
authors.lastname: Kudo
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D016232:Endothelins / Q000494:pharmacology*; D016257:Fura-2; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016232:Endothelins; D002118:Calcium; D016257:Fura-2