Enantioselective gas chromatographic assay with electron-capture detection for amlodipine in biological samples.
A sensitive enantioselective gas chromatographic assay has been developed for amlodipine, 2-[(2-aminoethoxy)-methyl]-4-(2-chlorophenyl)-3-ethoxycarbonyl-5- methoxycarbonyl-6-methyl-1,4-dihydropyridine, a calcium channel blocking therapeutic agent. The assay involves conversion of the (+)-(R)- and (-)-(S)-enantiomers of amlodipine into their acyl derivatives with the chiral reagent (+)-(S)-alpha-methoxy-alpha-trifluoromethylphenylacetyl chloride (Mosher's reagent). Peak separation after chromatography of the diastereomers was larger than 85%, and the lower limit of detection in blood plasma was 0.02 ng/ml for each enantiomer. The method has been used for the measurement of amlodipine enantiomers in human, rat and dog plasma, and in various organs of the rat.
Scharpf
F
F
R & D Laboratories, Pfizer Mack, Illertissen, Germany.
Riedel
K D
KD
Laufen
H
H
Leitold
M
M
D000328:Adult; D017311:Amlodipine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D004285:Dogs; D006801:Humans; D008168:Lung / Q000737:chemistry; D008297:Male; D009132:Muscles / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D051381:Rats; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D017311:Amlodipine