title: Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.
abstract: A cell line (SMKT-R3) established from human renal cell carcinoma was characterized for the presence of sulfolipids and glycolipid sulfotransferases. Sulfolipids were found to constitute a large part of the acidic glycolipid fraction in SMKT-R3 cells. These findings were confirmed by metabolic labelling with 35S-sulfate. These sulfolipids were expressed at the surface of SMKT-R3 cells as ascertained by cytofluorometry using a monoclonal antibody directed to sulfolipids. Furthermore, markedly high activity levels of glycolipid sulfotransferases were observed in SMKT-R3 cells compared with other cell lines. These results suggest that the increased synthesis of sulfolipids in renal cell carcinoma tissue (Sakakibara et al., 1989. Cancer Res., 49, 335-339) is due to the elevation of the sulfotransferase activities of renal carcinoma cells themselves.
authors.affiliation: Biochemistry Laboratory, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Honke
authors.forename: K
authors.initials: K
authors.lastname: Kamio
authors.forename: N
authors.initials: N
authors.lastname: Sakakibara
authors.forename: S
authors.initials: S
authors.lastname: Gasa
authors.forename: N
authors.initials: N
authors.lastname: Miyao
authors.forename: T
authors.initials: T
authors.lastname: Tsukamoto
authors.forename: I
authors.initials: I
authors.lastname: Ishizuka
authors.forename: T
authors.initials: T
authors.lastname: Miyazaki
authors.forename: A
authors.initials: A
authors.lastname: Makita
mesh_terms: D002292:Carcinoma, Renal Cell / Q000201:enzymology* / Q000378:metabolism; D002553:Cerebroside-Sulfatase / Q000378:metabolism; D005434:Flow Cytometry; D006017:Glycolipids / Q000378:metabolism; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D006017:Glycolipids; D008055:Lipids; C015518:sulfolipids; D013466:Sulfurtransferases; D015238:Sulfotransferases; C020556:galactosylceramide sulfotransferase; D002553:Cerebroside-Sulfatase