[The isolation of the bovine leukemia provirus by the polymerase chain reaction with subsequent nonradioactive blot hybridization].
A multiprobe scheme for detecting provirus of bovine leukemia virus (BLV) in the peripheral blood of infected animals was developed. According to the scheme, a fragment of BLV X-gene was amplified by polymerase chain reaction and detected by blot hybridization with the biotinylated oligonucleotide probe complementary to the inner part of the generated fragment. 600 copies of provirus may be specifically detected in a sample containing 150,000 cells. In some cases, BLV was detected in blood samples negative if tested by the commercial double immunodiffusion test.
Popov
A N
AN
Adarichev
V A
VA
Kalachikov
S M
SM
Mishina
E S
ES
Dymshits
G M
GM
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004279:DNA, Viral / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005814:Genes, Viral; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D015345:Oligonucleotide Probes
Vyiavlenie provirusa leĭkoza krupnogo rogatogo skota metodom polimeraznoĭ tsepnoĭ reaktsii s posleduiushcheĭ neradioaktivnoĭ blot-gibridizatsieĭ.