Cloning and expression of the major allergen from yellow mustard seeds, Sin a I.
The cloning and bacterial expression of the major allergen from yellow mustard (Sin a I) is reported. Cloning has been carried out by means of the polymerase chain reaction using non-degenerate oligo primers encoding the N- and C-terminal regions of the mature protein. Two nucleotide sequences (SA2S1 and SA2S2) have been found and analysed. The observed polymorphism suggests the existence of multiple isoforms for Sin a I allergen. SA2S2 has been expressed as a fusion protein, linked to the choline-binding domain of the Streptococcus pneumoniae murein hydrolase. The resulted fusion protein is recognized by Sin-a-I-specific antibodies.
González de la Peña
M A
MA
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense, Madrid, Spain.
Villalba
M
M
García-López
J L
JL
Rodríguez
R
R
D000485:Allergens / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D001665:Binding Sites; D015153:Blotting, Western; D002794:Choline / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009149:Mustard Plant / Q000235:genetics* / Q000276:immunology; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D010946:Plants, Medicinal; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000032:analysis; D012639:Seeds / Q000737:chemistry*; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics; D014169:Transformation, Bacterial
D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C079479:Sin a I protein, Sinapis alba; D009238:N-Acetylmuramoyl-L-alanine Amidase; D002794:Choline