[A comparative evaluation of an immunoenzyme analytical method using a fluorogenic or chromogenic substrate in determining the Marburg virus antigen].
A comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of Marburg virus antigen was carried out. The resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-8) g of protein for the latter substrate. Cross titrations demonstrated the lack of common antigens with Ebola virus.
Kutuzov
V A
VA
Mikhaĭlov
V V
VV
Kruglova
S E
SE
Kutuzov
V V
VV
Kirillov
A P
AP
Krasnianskiĭ
V P
VP
Pshenichnov
V A
VA
Lebedinskaia
E V
EV
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D029043:Ebolavirus / Q000276:immunology; D005069:Evaluation Studies as Topic; D006923:Hymecromone / Q000031:analogs & derivatives; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D029024:Marburgvirus / Q000276:immunology*; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D011817:Rabbits
D000956:Antigens, Viral; D007202:Indicators and Reagents; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; C005359:4-methylumbelliferyl phosphate; D006923:Hymecromone
Sravnitel'naia otsenka metoda immunofermentnogo analiza s primeneniem fliuorogennogo ili khromogennogo substrata pri opredelenii antigena virusa Marburg.