title: [Immunohistochemical detection of proliferating cell nuclear antigen (PCNA) in carcinoma at the confluence of the main hepatic ducts and its relationship to expression of CEA, CA19-9 and EGF receptor].
abstract: The Distribution pattern and proportion of the positive cells of proliferating cell nuclear antigen (PCNA) were examined immunohistochemically to study the proliferative activities of 33 cases of carcinoma found at the confluence of the main hepatic ducts. Expressions of CEA, CA19-9 and EGF receptor (R) were further examined with serial histological sections and comparatively analyzed with the result of PCNA staining and the clinico-pathological features of the carcinoma. PCNA positive cancer cells were observed in greatest abundance at the deeply infiltrated region of the carcinoma. CEA and CA19-9 were also expressed most strongly in this region and a stromal staining pattern was predominant. However, negative or apical staining patterns of CEA and CA19-9 were more frequent in the surface or the lateral spreading regions of the carcinoma, where the PCNA positive cancer cells were fewer in number. EGFR expression was rarely observed in the present study.
authors.affiliation: Department of Pathology, Tsukuba University.
authors.forename: T
authors.initials: T
authors.lastname: Kawamoto
authors.forename: K
authors.initials: K
authors.lastname: Saitou
authors.forename: T
authors.initials: T
authors.lastname: Todoroki
authors.forename: N
authors.initials: N
authors.lastname: Koike
authors.forename: N
authors.initials: N
authors.lastname: Kanazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000231:Adenocarcinoma, Papillary / Q000378:metabolism / Q000473:pathology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D001650:Bile Duct Neoplasms / Q000378:metabolism* / Q000473:pathology; D002272:Carcinoembryonic Antigen / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D006500:Hepatic Duct, Common; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D002272:Carcinoembryonic Antigen; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D066246:ErbB Receptors