Serum p53 auto-antibodies: incidence in familial breast cancer.
Inactivation of the p53 gene, which codes for a tumour suppressor protein, is known to occur in the majority of human malignancies. An ELISA technique has been developed which has detected auto-antibodies to p53 in the serum of 25.6% of 176 women with breast cancer, considerably higher than previously reported with an immunoblotting technique. The incidence of auto-antibodies in those cases with a family history of breast cancer was 9.1%, compared to 29.4% in those with no family history (P = 0.029). In women without clinical breast cancer, 4 out of 36 (11.1%) of those with a positive family history were seropositive, compared to 1 out of 73 control women. Auto-antibodies were more frequently seen in the serum of breast cancer patients whose biopsies demonstrated overexpression of p53 protein. We conclude that auto-antibodies to p53 may have a role in the molecular characterisation of familial breast cancer.
Green
J A
JA
Department of Medicine, Royal Liverpool University Hospital, U.K.
Mudenda
B
B
Jenkins
J
J
Leinster
S J
SJ
Tarunina
M
M
Green
B
B
Robertson
L
L
D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000097:blood* / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000276:immunology; D009386:Neoplastic Syndromes, Hereditary / Q000097:blood*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000276:immunology*
D001323:Autoantibodies; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53