A revised map position for the Ha-ras gene on mouse chromosome 7: implications for analysis of genetic alterations in rodent tumors.
The mouse Ha-ras gene has previously been mapped to the central region of chromosome 7, 31 cM from the centromere, using an interspecific Mus musculus/Mus spretus backcross (Saunders AM, Seldin MF, Genomics 8:525-535, 1990). However, analysis of mitotic recombinations in mouse skin tumors from intraspecific F1 hybrid mice suggested a more distal location for the Ha-ras gene on chromosome 7 (Bremner R, Balmain A, Cell 61:407-417, 1990). In the study reported here, we demonstrated, by analysis of Ha-ras gene mutations in skin tumors from interspecific M. spretus/M. musculus F1 hybrids, the existence only in M. spretus of a pseudogene or other Ha-ras-related sequence that is probably the sequence originally mapped by Saunders and Seldin. The functional Ha-ras gene maps to the distal region of chromosome 7, and it is this sequence that acquires mutations in chemically induced tumors.
Kemp
C J
CJ
CRC Beatson Laboratories, Beatson Institute for Cancer Research, Glasgow, United Kingdom.
Bremner
R
R
Balmain
A
A
D000818:Animals; D002869:Chromosome Aberrations / Q000235:genetics; D025063:Chromosome Disorders; D002874:Chromosome Mapping; D011905:Genes, ras; D051379:Mice; D009374:Neoplasms, Experimental / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D011544:Pseudogenes
D016283:Proto-Oncogene Proteins p21(ras)