[The detection of provirus in lymphocyte DNA. The monitoring of HIV infection at the genetic level].
The presence of HIV provirus in the cell culture and in the patients' blood was studied by polymerase chain reaction followed by hybridization in solution. It was shown that: (i) the hybridized product could be detected both by gel electrophoresis and by binding on hydroxyapatite; (ii) the detection level achieved was no more than 10 infected lymphocytes per million; (iii) the hybridization signal and sensitivity of detection could be enhanced by the transcription of PCR product by the phage T7 RNA polymerase. The observed lack of complete correlation between the amount of provirus and of the p24 antigen in the patients' blood possibly reflects the peculiarities of HIV infection.
Elov
A A
AA
Kozlova
A V
AV
Mednikov
B M
BM
Kornilaeva
G V
GV
Papuashvili
M N
MN
Prokopenko
V D
VD
Karamov
E V
EV
D002478:Cells, Cultured; D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D005820:Genetic Testing / Q000379:methods*; D015658:HIV Infections / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011533:Proviruses / Q000235:genetics*
D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral
Detektsiia provirusa v DNK limfotsitov. Monitoring VICh-infektsii na geneticheskom urovne.