title: Comparative nuclear magnetic resonance studies on water diffusional permeability of red blood cells from mice and rats.
abstract: 1. The diffusional water permeability (P) of mouse and rat red blood cell (RBC) membrane has been monitored by a doping nuclear magnetic resonance (NMR) technique on control cells and following exposure to various concentrations of p-chloromercuribenzene sulfonate (PCMBS). 2. There were no significant differences in the water permeability of mouse and rat RBCs, the values of P being around 6 x 10(-3)/sec at 20 degrees C and 11 x 10(-3) cm/sec at 37 degrees C. 3. Systematic studies of the effects of PCMBS on water diffusion indicated that the maximal inhibition is reached in 60 min at 20 degrees C with 1 mM PCMBS for the mouse RBCs and with 2 mM PCMBS for the rat RBCs. 4. The values of maximal inhibition ranged from 55-57% at 37 degrees C and reached values around 70% at 10 degrees C. 5. The degree of inhibition increased as the temperature of measurement decreased, regardless of PCMBS concentration and incubation time. 6. The basal permeability to water of mouse RBCs was estimated as 1.8 x 10(-3) cm/sec at 20 degrees C and 4.6 x 10(-3) cm/sec at 37 degrees C, and that of rat RBCs as 2.2 x 10(-3) cm/sec at 20 degrees C and 4.2 x 10(-3) cm/sec at 37 degrees C. 7. In both species the activation energy was around 27 kJ/mol and reached values over 40 kJ/mol after incubation with PCMBS in the conditions of maximal inhibition of water diffusion.
authors.affiliation: Department of Cell and Molecular Biology, University of Medicine and Pharmacy Cluj-Napoca, Romania.
authors.forename: G
authors.initials: G
authors.lastname: Benga
authors.forename: H
authors.initials: H
authors.lastname: Matei
authors.forename: T
authors.initials: T
authors.lastname: Borza
authors.forename: D
authors.initials: D
authors.lastname: Poruţiu
authors.forename: C
authors.initials: C
authors.lastname: Lupşe
mesh_terms: D002731:4-Chloromercuribenzenesulfonate / Q000378:metabolism; D000818:Animals; D001834:Body Water / Q000378:metabolism*; D002463:Cell Membrane Permeability; D004058:Diffusion; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D051381:Rats; D013696:Temperature
chemical_list: D002731:4-Chloromercuribenzenesulfonate