Recombinant vaccinia viruses expressing interleukin-5 stimulate an earlier appearance of antibody-secreting cells in the lung.
Interleukin-5 (IL-5) is a cytokine that participates in the regulation of antibody secretion, in particular promoting the secretion of IgA at mucosal sites. In this report, recombinant vaccinia viruses expressing IL-5 have been inoculated into mice and the appearance of antibody-secreting cells in the spleen and lungs investigated. Although vaccinia virus-expressed IL-5 did not increase the level of IgA in serum, antibody-secreting cells, measured in an enzyme-linked immunosorbent spot assay, appeared earlier in lungs when the immunizing virus expressed IL-5. These early B cells secreted either IgM or IgG1.
Janardhana
V
V
CSIRO Australian Animal Health Laboratory, Geelong, Victoria.
Andrew
M
M
Thomas
S
S
Coupar
B
B
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors; D015848:Interleukin-5 / Q000235:genetics / Q000276:immunology*; D008168:Lung / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014616:Vaccinia virus
D015848:Interleukin-5; D011994:Recombinant Proteins