title: Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica.
abstract: The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.
authors.affiliation: Department of Biochemistry, University of Dundee, Scotland, UK.
authors.forename: A
authors.initials: A
authors.lastname: Mehlert
authors.forename: L
authors.initials: L
authors.lastname: Varon
authors.forename: I
authors.initials: I
authors.lastname: Silman
authors.forename: S W
authors.initials: SW
authors.lastname: Homans
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson
mesh_terms: D000110:Acetylcholinesterase / Q000096:biosynthesis*; D000818:Animals; D002240:Carbohydrate Sequence; D004557:Electric Organ / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D017261:Glycosylphosphatidylinositols / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D014101:Torpedo
chemical_list: D017261:Glycosylphosphatidylinositols; D000110:Acetylcholinesterase