On mechanism of action of ethanol-induced contraction of frog rectus abdominis.
Ethanol in low doses (0.5 to 4 M) causes contraction of isolated frog rectus abdominis muscle. Higher concentration did not produce any further increase in maximum response. Pretreatment with dantrolene produced partial but equal inhibition of acetylcholine (Ach) induced as well as ethanol-induced contraction in equieffective doses. Pretreatment with pancuronium produced right and downward shift of ethanol induced contraction. Pretreatment with succinylcholine produced persistent contraction of tissue and this response remained unaffected on subsequent treatment with Ach as well as ethanol. Pretreatment with hemicholinium abolished ethanol induced contraction, although tissue remained viable as confirmed on addition of Ach. The contraction induced by ethanol decreased on pretreatment with dantrolene as well as in Ca2+ free ringer. The results indicate that ethanol induced contraction may be due to release of Ach or Ach like neurotransmitter at neuromuscular junction and calcium acts as mediator to produce these effects.
Kela
A K
AK
Department of Pharmacology, Lady Hardinge Medical College, New Delhi, India.
Sharma
A K
AK
Mehta
S C
SC
Mehta
V L
VL
Srivastava
A K
AK
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003620:Dantrolene / Q000494:pharmacology; D004347:Drug Interactions; D000431:Ethanol / Q000494:pharmacology*; D006426:Hemicholinium 3 / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects*; D010197:Pancuronium / Q000494:pharmacology; D011898:Ranidae; D013390:Succinylcholine / Q000494:pharmacology
D006426:Hemicholinium 3; D000431:Ethanol; D003620:Dantrolene; D013390:Succinylcholine; D010197:Pancuronium; D000109:Acetylcholine