title: Enantioselective gas chromatographic assay with electron-capture detection for amlodipine in biological samples.
abstract: A sensitive enantioselective gas chromatographic assay has been developed for amlodipine, 2-[(2-aminoethoxy)-methyl]-4-(2-chlorophenyl)-3-ethoxycarbonyl-5- methoxycarbonyl-6-methyl-1,4-dihydropyridine, a calcium channel blocking therapeutic agent. The assay involves conversion of the (+)-(R)- and (-)-(S)-enantiomers of amlodipine into their acyl derivatives with the chiral reagent (+)-(S)-alpha-methoxy-alpha-trifluoromethylphenylacetyl chloride (Mosher's reagent). Peak separation after chromatography of the diastereomers was larger than 85%, and the lower limit of detection in blood plasma was 0.02 ng/ml for each enantiomer. The method has been used for the measurement of amlodipine enantiomers in human, rat and dog plasma, and in various organs of the rat.
authors.affiliation: R & D Laboratories, Pfizer Mack, Illertissen, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Scharpf
authors.forename: K D
authors.initials: KD
authors.lastname: Riedel
authors.forename: H
authors.initials: H
authors.lastname: Laufen
authors.forename: M
authors.initials: M
authors.lastname: Leitold
mesh_terms: D000328:Adult; D017311:Amlodipine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D004285:Dogs; D006801:Humans; D008168:Lung / Q000737:chemistry; D008297:Male; D009132:Muscles / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D051381:Rats; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
chemical_list: D017311:Amlodipine