[Quantitative cytochemical analysis of T1 breast cancer].
The authors attempted to estimate the relationship between three biological parameters (nuclear DNA content, PCNA (proliferating cell nuclear antigen)/cyclin, HER-2/neu oncoprotein) and lymph node metastasis. We evaluated 37 breast cancers which were less than 2 cm in maximum dimension. Quantitative analysis was performed using a CAS 200 Image Analysis System, after Feulgen staining and immunochemical staining using anti-PCNA/cyclin monoclonal antibody and anti-HER-2/neu oncoprotein polyclonal antibody. In lymph node-negative cases 20.0% were aneuploid, while in lymph node-positive cases 58.8% were aneuploid. A total of 20.0% lymph node-negative cases were in the high proliferation group, as opposed to 52.9% of lymph node-positive cases. This analysis revealed a significant relationship between cell proliferation and lymph node metastasis. Analysis of the expression of HER-2/neu oncoprotein revealed no significant relationship between overexpression of HER-2/neu oncoprotein and lymph node metastasis, but the expression of HER-2/neu oncoprotein was significantly related to a shorter relapse-free survival.
Ikeda
N
N
Dept. of Surgery I, Tokyo Medical College.
Hirano
T
T
Okuzawa
K
K
Uchida
O
O
Katsumi
T
T
Kajiwara
N
N
Hara
E
E
Miura
H
H
Kawate
N
N
Konaka
C
C
D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008207:Lymphatic Metastasis; D009687:Nuclear Proteins / Q000032:analysis; D009856:Oncogene Proteins, Viral / Q000032:analysis; D011003:Ploidies; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen
D004273:DNA, Neoplasm; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D018809:Proliferating Cell Nuclear Antigen