Serological differentiation of human papillomavirus types 11, 16 and 18 using recombinant virus-like particles.
The L1 major capsid protein-coding sequences of human papillomavirus (HPV) types 11, 16 and 18 were expressed in the baculovirus system. Virus-like particles (VLPs) were purified from recombinant-infected Spodoptera frugiperda Sf9 cells and cell-free culture supernatants. Rabbits immunized with purified VLPs developed antibodies that reacted only with the specific VLP type used as the immunogen. In addition, rabbit antibodies raised against infectious HPV-11 virions only reacted with HPV-11 L1 VLPs and not with VLPs derived from either HPV-16 or HPV-18. These results suggest that HPV-11, HPV-16 and HPV-18 virions are antigenically distinct from one another. This observation should be considered in future studies of immune responses to HPV.
Rose
R C
RC
Department of Medicine, University of Rochester School of Medicine and Dentistry, New York 14642.
Bonnez
W
W
Da Rin
C
C
McCance
D J
DJ
Reichman
R C
RC
D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D002213:Capsid / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D009036:Moths; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D014771:Virion / Q000145:classification / Q000235:genetics / Q000276:immunology
D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins