VIP potentiates retinoic-acid effect on tissue transglutaminase activity in human neuroblastoma, the SK-N-SH cells.
Tissue transglutaminase (tTG) activity was used to test the potent regulatory role of vasoactive intestinal peptide (VIP) on Retinoic Acid-induced effect in human neuroblastoma cell line. The comparison between both differentiation and cell death related to tissue transglutaminase was discussed in this model. VIP alone was a potent differentiating agent in SK-N-SH cells but in the presence of retinoic acid (RA), this peptide rather potentiates RA-induced tTG activity which is now considered as an apoptosis marker in neuroblastoma cell line. This paper demonstrated an additional neuromodulator role for VIP.
Goossens
J F
JF
INSERM U16, Lille, France.
Manechez
D
D
Pommery
N
N
Formstecher
P
P
Hénichart
J P
JP
D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009447:Neuroblastoma / Q000201:enzymology*; D011503:Transglutaminases / Q000096:biosynthesis*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D015415:Biomarkers; D009363:Neoplasm Proteins; D014660:Vasoactive Intestinal Peptide; D014212:Tretinoin; D011503:Transglutaminases