title: Platelet size and glycoprotein Ib and IIIa expression in normal fetal and maternal blood.
abstract: OBJECTIVE
  Our purpose was to study platelet size and surface glycoprotein expression in normal fetal and maternal blood throughout pregnancy.
  
  
  METHODS
  A cross-sectional study was performed at the Harris Birthright Research Centre for Fetal Medicine, King's College Hospital Medical School, London. Fetal and maternal blood samples were obtained from uncomplicated pregnancies at 8 to 42 weeks' gestation (n = 101 and n = 117, respectively) and from 30 nonpregnant controls. Flow cytometry was used to determine platelet size and glycoprotein Ib and IIIa expression both before and after stimulation with adenosine diphosphate.
  
  
  RESULTS
  Mean platelet size in both fetal and maternal blood was significantly lower than that of nonpregnant controls and decreased with advancing gestation. The surface density of glycoprotein Ib in maternal and fetal platelets was significantly lower than in nonpregnant controls but did not change with gestation. Adenosine diphosphate stimulation of maternal platelets resulted in increased percentage expression and surface density of all glycoproteins, whereas stimulation of control platelets resulted in increased surface density of glycoprotein Ib and percentage expression of glycoprotein IIIa. Adenosine diphosphate stimulation of fetal platelets resulted in increased surface density of glycoprotein Ib and IIIa.
  
  
  CONCLUSIONS
  Pregnancy is associated with increased thrombocytopoiesis in both the mother and fetus. Maternal platelet glycoprotein expression and responsiveness to adenosine diphosphate stimulation is increased. Fetal platelets are phenotypically mature from at least 12 weeks' gestation and respond in an adultlike fashion to stimulation with adenosine diphosphate.
authors.affiliation: Department of Obstetrics and Gynaecology, Harris Birthright Research Centre for Fetal Medicine, King's College School of Medicine, London, United Kingdom.
authors.forename: N J
authors.initials: NJ
authors.lastname: Meher-Homji
authors.forename: R
authors.initials: R
authors.lastname: Montemagno
authors.forename: B
authors.initials: B
authors.lastname: Thilaganathan
authors.forename: K H
authors.initials: KH
authors.lastname: Nicolaides
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D048429:Cell Size; D017218:Cordocentesis; D003430:Cross-Sectional Studies; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D005865:Gestational Age; D006801:Humans; D010980:Platelet Membrane Glycoproteins / Q000097:blood*; D011247:Pregnancy / Q000097:blood* / Q000187:drug effects; D012044:Regression Analysis; D015854:Up-Regulation
chemical_list: D010980:Platelet Membrane Glycoproteins; D000244:Adenosine Diphosphate