Floral organ-specific and constitutive expression of an Arabidopsis thaliana heat-shock HSP18.2::GUS fusion gene is retained even after homeotic conversion of flowers by mutation.
Organ-specific and constitutive expression of the Arabidopsis HSP18.2 gene under normal growth conditions (22 degrees C) was observed in transgenic A. thaliana, which carried a fusion gene composed of the promoter region of HSP18.2, one of the genes for low molecular weight heat-shock proteins in Arabidopsis, and the gene for beta-glucuronidase (GUS) from Escherichia coli. In order to clarify the organ-specific nature of promoter expression, various mutations that affect flower morphology were introduced into the transgenic Arabidopsis line, AHS9. The results show that the pattern of expression observed in sepals, filaments, and styles is regulated in a structure-dependent manner, and suggest that the HSP18.2 gene might have an important role in the process of differentiation of flower buds, as do several other stress-related genes.
Tsukaya
H
H
University of Tokyo, Molecular Genetics Research Laboratory, Japan.
Takahashi
T
T
Naito
S
S
Komeda
Y
Y
D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D005801:Genes, Homeobox / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006651:Histocytochemistry; D006358:Hot Temperature; D009024:Morphogenesis / Q000235:genetics*; D016296:Mutagenesis; D009928:Organ Specificity; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification
D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D005966:Glucuronidase