Morphological and functional alterations to a sub-group of regulatory peptides in human pancreas and intestine after jejuno-ileal bypass.
The effect of ileo-gastrostomy on insulin and cholecystokinin secretion and the endocrine cells containing cholecystokinin, somatostatin, motilin, secretin, neurotensin and enteroglucagon was studied. Fasting and postprandial plasma samples collected pre- and post-operation demonstrated significant changes in circulating insulin and cholecystokinin. Fasting and postprandial insulin levels decreased three months after ileo-gastrostomy (postprandial pre-operation 40 +/- 8 microU/ml compared to 15 +/- 24 microU/ml post-operation, P < 0.02). The postprandial levels of cholecystokinin were significantly increased (pre-operation 7.1 +/- 1.1 pg/ml compared to 12.2 +/- 1.7 pg/ml post-operation, P < 0.02). Quantification of the endocrine cell populations in the jejunum in-continuity three months after ileo-gastrostomy demonstrated an hyperplasia of cholecystokinin-, secretin-, gastric inhibitory polypeptide-, motilin- and somatostatin-containing cells. In samples of the ileum taken from within the bypass loop the neurotensin- and somatostatin-containing cells were unaffected while the enteroglucagon-containing cells were significantly increased in number. Ileo-gastrostomy resulted in significant alterations to the abundance of regulatory peptide-containing endocrine cells and to circulating levels of insulin and cholecystokinin. These changes are implicated in the dramatic weight loss associated with the operation.
Buchan
A M
AM
Department of Physiology, University of British Columbia, Vancouver, Canada.
Pederson
R A
RA
Koop
I
I
Gourlay
R H
RH
Cleator
I G
IG
D000328:Adult; D002766:Cholecystokinin / Q000378:metabolism; D005215:Fasting; D005260:Female; D005502:Food; D004763:Glucagon-Like Peptides / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000378:metabolism; D007422:Intestines / Q000033:anatomy & histology*; D007581:Jejunoileal Bypass; D008297:Male; D009037:Motilin / Q000378:metabolism; D009496:Neurotensin / Q000378:metabolism; D010179:Pancreas / Q000033:anatomy & histology* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011863:Radioimmunoassay; D012633:Secretin / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism
D007328:Insulin; D010455:Peptides; D012633:Secretin; D009496:Neurotensin; D013004:Somatostatin; D009037:Motilin; D004763:Glucagon-Like Peptides; D002766:Cholecystokinin