title: [Quantification of the enantiomers of ofloxacin in human urine by RP-HPLC with chiral mobile phase additive].
abstract: The method for determination of S-(-) and R-(+)-ofloxacin (OFLX) in human urine by RP-HPLC with a chiral mobile phase additive is described. L-phenylalanine was used as the chiral additive, Cu2+ as the ligand ion and fluorescence for detection. This chiral ligand exchange chromatography has been applied to reveal the enantioselective disposition of ofloxacin in human volunteers.
authors.affiliation: School of Pharmacy, Zhejiang Medical University, Hangzhou.
authors.forename: S
authors.initials: S
authors.lastname: Zeng
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Liu
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D015242:Ofloxacin / Q000652:urine*; D013237:Stereoisomerism
chemical_list: D015242:Ofloxacin