GreA-induced transcript cleavage is accompanied by reverse translocation to a different transcription complex conformation.
GreA- and GreB-induced transcript cleavage drives reverse translocation of Escherichia coli RNA polymerase on a DNA template in the absence of NTPs (Feng, G.-H., Lee, D. N., Wang, D., Chan, C. L., and Landick, R. (1994) J. Biol. Chem. 269, 22282-22294, accompanying report). During transcript elongation, the sizes of the DNA footprint and the single-stranded transcription bubble vary markedly among transcription complexes halted at different template positions. To test whether transcription complex intermediates formed during transcript cleavage-induced reverse translocation also display heterogeneous conformations at different template positions, we examined the structures of two different transcription complexes before and after GreA treatment. Transcription complexes halted at position +16 after initiation at the T7 A1 promoter or paused at the trpL pause site exhibited strong blocks to transcript cleavage after removal of 6 to 10 nucleotides. In both cases, the down-stream contact between RNA polymerase and DNA moved little during transcript cleavage, thereby increasing its distance from the active site, whereas the upstream DNA contact and the borders of the transcription bubble moved in approximate register with the transcript 3'-end. The backward movements of halted E. coli RNA polymerase are similar to a recently postulated model for discontinuous translocation during transcription, but differ from those reported for arrested RNA polymerase II transcription complexes.
Lee
D N
DN
Department of Biology, Washington University, St. Louis, Missouri 63130.
Feng
G
G
Landick
R
R
D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011196:Potassium Permanganate; D011487:Protein Conformation; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D013698:Templates, Genetic; D014157:Transcription Factors; D014158:Transcription, Genetic; D014364:Tryptophan / Q000378:metabolism
D001426:Bacterial Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D011196:Potassium Permanganate; C075886:GREA protein, Rickettsia prowazekii; D014364:Tryptophan; D012321:DNA-Directed RNA Polymerases