Expression of the galactose-binding lectins during the formation of organ primordia in the chick embryo.
Early chick embryos contain two beta-galactoside-binding lectins of 16 kDa and 14 kDa. using several antisera to these proteins, we have studied lectin expression at embryonic stages when the segregation and early differentiation of organ primordia are taking place. With antisera to the 16 kDa lectin that display similar immunoreactivity in immunoblot analysis, we show that these antisera exhibit varying immunoreactivity in embryo sections. One antiserum reacts preferentially with a matrix form of lectin while another detects mainly a cellular form of this protein. During early development, galactoside-binding lectins of the matrix type are expressed in the vitelline membrane, the outer and inner limiting membranes of the neural tube, the surface of the notochord and the coelomic surface of the cardiac rudiments. The cellular form of the lectin occurs in the intracellular yolk of early embryos, in the primordial germ cells, the myocardium, in the early myotome, and in a cohort of cells which are presumed to belong to the neural crest. Our results indicate that, although all of the antisera recognize the intracellular lectin of the extraembryonic endoderm, some antisera to the 16 kDa lectin exhibit preferential reactivity with different lectin isoforms. The extracellular matrix form of lectin is transiently expressed during early development at the stages when the segregation of organ primordia is occurring. It's expression could be related to the acquisition of polarity in developing epithelia. Results also suggest that various versions of the same protein may perform distinct developmental roles in the embryo.
Zalik
S E
SE
Department of Zoology, University of Alberta, Canada.
Didier
E
E
Didier
P
P
Ledsham
I M
IM
Bayle
D
D
Sanders
E J
EJ
D000818:Animals; D001756:Blastoderm / Q000166:cytology / Q000502:physiology; D002642:Chick Embryo / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D005314:Embryonic and Fetal Development; D005697:Galactosides / Q000378:metabolism; D037161:Galectins; D006388:Hemagglutinins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D007106:Immune Sera; D015151:Immunoblotting; D007150:Immunohistochemistry / Q000379:methods; D008648:Mesoderm / Q000166:cytology / Q000502:physiology; D016253:Microscopy, Immunoelectron / Q000379:methods; D008970:Molecular Weight
D005697:Galactosides; D037161:Galectins; D006388:Hemagglutinins; D007106:Immune Sera