Extraction, partial purification and characterization of a bacteriocin (fragicilin) produced by a strain of Bacteroides fragilis isolated from Callithrix penicillata.
A strain of Bacteroides fragilis, isolated from the marmoset Callithrix penicillata, produced protein(s) with bacteriocin activity (fragicilin). Two active fractions (36 and 150 kDa) were isolated by chromatography. The bacteriocin exhibited iso- and heteroantagonism. It remained stable between pH 3 and 10 and at 60 degrees C for 24 h. Pronase, trypsin, proteinase K and type VII protease inactivated the bacteriocin, giving evidence of its protein nature.
Farias
L M
LM
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Totola
A H
AH
Miranda
C M
CM
Carvalho
M A
MA
Damasceno
C A
CA
Tavares
C A
CA
Cisalpino
E O
EO
Vieira
E C
EC
D000818:Animals; D001430:Bacteriocins / Q000302:isolation & purification* / Q000494:pharmacology; D001441:Bacteroides fragilis / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification; D002144:Callithrix / Q000382:microbiology*; D002847:Chromatography, Agarose; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D013297:Streptococcus pyogenes / Q000187:drug effects; D013298:Streptococcus sanguis / Q000187:drug effects; D013696:Temperature
D001430:Bacteriocins