Serotonin, histamine and somatostatin modulation of PMA-stimulated phosphorylation of 130 kDa Ca2+ pump-like protein from rat cerebellum synaptosomal membranes.
1. Influence of some neurotransmitters and neuromodulators on the PMA-stimulated phosphorylation in vitro of calcium pump-like protein from rat cerebellum synaptosomal membranes was examined. 2. The prolonged time (up to 6 min) of synaptosomal membranes preincubation with 1 and 10 microM serotonin results in the increase of phosphorylation. The decrease of phosphorylation up to 80% of control value was observed for 100 microM serotonin. 3. The most stimulating effect on 130 kDa protein phosphorylation was observed with 1 microM of histamine (160% of control value). 4. 1 and 0.1 microM somatostatin triggered a short-time transient increase of 130 kDa phosphorylation (up to 135% of control value).
Zylińska
L
L
II Department of Biochemistry, Medical University of Lódź, Poland.
Lachowicz
L
L
D000818:Animals; D000252:Calcium-Transporting ATPases; D002531:Cerebellum / Q000737:chemistry*; D006632:Histamine / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*; D013004:Somatostatin / Q000494:pharmacology*; D013570:Synaptic Membranes / Q000737:chemistry*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D010750:Phosphoproteins; D012701:Serotonin; D013004:Somatostatin; D006632:Histamine; D000252:Calcium-Transporting ATPases; D013755:Tetradecanoylphorbol Acetate