title: Genomic structure, chromosomal location, and evolution of the mouse Hox 8 gene.
abstract: We isolated genomic clones containing the mouse Hox 8 gene, a member of the msh gene family. We show that Hox 8 comprises two exons of approximately 600 and 691 bp separated by a 3.5-kb intron, and that it cosegregates with previously mapped markers in the distal region of mouse chromosome 13. In midgestation embryos, the Hox 8 gene produces transcripts of 1.4 and 2.2 kb. Both transcripts are present in facial tissues of the newborn mouse, though the ratio of the 2.2-kb transcript to the 1.4-kb transcript is reduced relative to the ratio observed for midgestation embryos. An alignment of the homeobox sequences of previously characterized members of the msh family revealed three subclasses: Hox 7-like genes, Hox 8-like genes, and msh-like genes. Both the Hox 7-like genes and Hox 8-like genes are present throughout the vertebrates. Representatives of the third subclass, the msh-like genes, are found in a protostome (Drosophila) and a deuterostome (Ciona) and are thus likely to be phylogenetically widespread. To investigate the distribution of Hox 8-like genes outside the chordates, we used the polymerase chain reaction and degenerate Hox 8 primers to screen genomic DNA of the purple sea urchin (Strongylocentrotus purpuratus, Phylum Echinodermata). We isolated a gene with greater sequence similarity to mouse Hox 8 than to members of the Hox 7 or msh subfamilies, demonstrating that the Hox 8 subfamily has been in existence at least since the echinoderms diverged from the lineage that gave rise to the chordates.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern California School of Medicine, Los Angeles 90033.
authors.forename: J R
authors.initials: JR
authors.lastname: Bell
authors.forename: A
authors.initials: A
authors.lastname: Noveen
authors.forename: Y H
authors.initials: YH
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Ma
authors.forename: S
authors.initials: S
authors.lastname: Dobias
authors.forename: R
authors.initials: R
authors.lastname: Kundu
authors.forename: W
authors.initials: W
authors.lastname: Luo
authors.forename: Y
authors.initials: Y
authors.lastname: Xia
authors.forename: A J
authors.initials: AJ
authors.lastname: Lusis
authors.forename: M L
authors.initials: ML
authors.lastname: Snead
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005801:Genes, Homeobox; D008040:Genetic Linkage; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012617:Sea Urchins / Q000235:genetics
chemical_list: D004247:DNA