An investigation of urinary creatine excretion as a potential marker for testicular damage.
1. This study assessed urinary creatine excretion as a marker for testicular atrophy. 2. Male rats received a single i.p. dose of 2-methoxyethanol at 0, 250 or 750 mg kg-1 and were sacrificed 2 d later. Urinary creatine and creatinine excretion were measured on days 0, 1 and 2. 3. Decreased testicular weights and histopathological assessment revealed dose-related testicular damage. 4. On day 1, at both doses of 2-methoxyethanol, urinary creatine levels increased and creatinine levels decreased, resulting in a dose-related increase in the creatine/creatinine ratio. On day 2, the creatine/creatinine ratio was elevated relative to controls, but was less marked than on day 1. 5. The study confirmed that creatine excretion is a potential marker for acute testicular damage.
Nahas
K
K
Laboratoires Pfizer, Centre de Recherche, Amboise, France.
le Net
J L
JL
Provost
J P
JP
Tomaszewski
K E
KE
D000818:Animals; D001284:Atrophy / Q000139:chemically induced; D015415:Biomarkers / Q000652:urine; D003401:Creatine / Q000652:urine*; D003404:Creatinine / Q000652:urine; D005026:Ethylene Glycols / Q000633:toxicity; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013733:Testicular Diseases / Q000139:chemically induced / Q000473:pathology / Q000652:urine*
D015415:Biomarkers; D005026:Ethylene Glycols; D003404:Creatinine; C005219:methyl cellosolve; D003401:Creatine