cDNA cloning of a novel protein tyrosine phosphatase with homology to cytoskeletal protein 4.1 and its expression in T-lineage cells.
Reversible tyrosine phosphorylation plays important regulatory roles in various cellular events including the differentiation and function of lymphocytes. Here we report the cDNA cloning of a non-receptor type protein tyrosine phosphatase, PTP36, which is expressed in murine thymus. PTP36 was a new member of a tyrosine phosphatase subfamily defined by MEG-01 and PTPH1, which had a C-terminal phosphatase domain as well as an N-terminal domain with homology to cytoskeletal-associated proteins like band 4.1, ezrin, and talin. In addition, we found a putative SH3-binding motif in PTP36 but not in MEG-01 or PTPH1. PTP36 was expressed in cells of both hematopoietic and non-hematopoietic origins. In thymocytes subpopulations, PTP36 was preferentially expressed in double positive stage cells. The change of PTP36 expression level along with T cell maturation suggests its involvement in the regulation of T cell development.
Sawada
M
M
Biomedical Research Center, Osaka University Medical School, Japan.
Ogata
M
M
Fujino
Y
Y
Hamaoka
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D008969:Molecular Sequence Data; D009479:Neuropeptides; D010750:Phosphoproteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis* / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000201:enzymology*; D016608:Talin / Q000235:genetics; D013950:Thymus Gland / Q000201:enzymology; D000099149:Ezrin
D003598:Cytoskeletal Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D008565:Membrane Proteins; D009479:Neuropeptides; D010750:Phosphoproteins; D017027:Protein Tyrosine Phosphatases; D016608:Talin; D000099149:Ezrin; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1