Hypothalamic neuropeptide Y and its gene expression: relation to light/dark cycle and circulating corticosterone.
The hypothalamic neuropeptide Y (NPY) system, along with levels of circulating corticosterone (CORT), were examined in rats at different times across the light/dark cycle. Tissue samples were taken from the mediobasal hypothalamus (MBH), which contains the primary hypothalamic NPY cell group of the arcuate nucleus (ARC), and the mediodorsal (MDH) hypothalamus, which contains the paraventricular and dorsomedial nuclei that receive a dense NPY innervation from the ARC. In these dissections, measurements of NPY mRNA and peptide levels were taken using a solution hybridization/nuclease protection assay procedure and radioimmunoassay. The results demonstrate that (i) NPY mRNA levels in the MBH, but not MDH, vary significantly in relation to the light/dark cycle, showing a sharp rise 4-6 h before dark onset, sustained high levels over the next 3-4 h and then, a sharp decline 1 h before dark onset; (ii) this rise in NPY mRNA in the MBH before dark onset, while associated with stable levels of MBH NPY during this time, is followed 2-4 h later, around dark onset, by a rise in NPY peptide levels of the MDH simultaneous to a decrease in NPY levels of the MBH; (iii) levels of circulating CORT shift dramatically across the light-dark cycle, exhibiting an increase from basal levels (< 0.3 microgram/dl) to 5 micrograms/dl approximately 4 h before dark onset, a further rise that peaks at 26 micrograms/dl around dark onset, and then a significant decline to 16 micrograms/dl at 2 h after dark onset; and (iv) there exists a positive relationship between CORT and NPY mRNA or peptide levels in the MBH during the 4-6 h before dark onset, while in the MDH, a positive relationship between this steroid and NPY peptide levels is obtained at dark onset. It is proposed that these rhythms, involving a predark rise in CORT and NPY gene expression leading to a peak in CORT and peptide levels at dark onset, are active in stimulating feeding behavior, particularly carbohydrate ingestion, which predominates at that time.
Akabayashi
A
A
Rockefeller University, New York, New York 10021.
Levin
N
N
Paez
X
X
Alexander
J T
JT
Leibowitz
S F
SF
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000450:Aldosterone / Q000097:blood; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis; D002940:Circadian Rhythm / Q000502:physiology*; D003345:Corticosterone / Q000097:blood* / Q000378:metabolism; D003624:Darkness; D004040:Dietary Carbohydrates; D005247:Feeding Behavior / Q000502:physiology; D005786:Gene Expression Regulation; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology; D007031:Hypothalamus / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D008297:Male; D009478:Neuropeptide Y / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D010913:Pituitary-Adrenal System / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D007328:Insulin; D009478:Neuropeptide Y; D012333:RNA, Messenger; D000450:Aldosterone; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone