Small bowel transplantation. The effect of intraportal donor-specific transfusion 24 hours pretransplant and low-dose cyclosporine.
We tested the synergy of donor-specific transfusion (DST) and cyclosporine (CsA) in small bowel transplantation by comparing the systemic versus portal route of DST administration in a fully allogeneic rat model. The protocol is relevant to cadaveric transplantation by conditioning only 24 hr before allografting. A 1 ml intraportal DST day -1 and low-dose CsA significantly prolonged survival (MST 53.7 +/- 17.5 days) when compared with systemic DST day -1 and low-dose CsA (MST 18.4 +/- 5.6 days). This suggests that intraportal DST can be beneficial in cadaveric SBT, as only a 24-hr induction period is necessary. We speculate that antigen trapping in the liver and interaction of the DST with Kupffer cells is central to the portal DST effect.
Fecteau
A H
AH
Department of Surgery, McGill University, Montreal, Canada.
Tchervenkov
J I
JI
Guttman
F M
FM
Takara
T
T
Rosenmann
E
E
D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D001803:Blood Transfusion / Q000379:methods; D016572:Cyclosporine / Q000008:administration & dosage*; D006085:Graft Survival / Q000276:immunology*; D007279:Injections, Subcutaneous; D007421:Intestine, Small / Q000276:immunology* / Q000637:transplantation*; D007728:Kupffer Cells / Q000276:immunology; D008099:Liver / Q000276:immunology; D008297:Male; D011169:Portal Vein; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous
D016572:Cyclosporine