A conserved sequence in tRNA and rRNA promoters of Lactococcus lactis.
A tRNA operon (trnA) from Lactococcus lactis consisting of seven tRNA genes and a 5S rRNA gene was cloned and sequenced. Promoter-fusion of the trnA promoter to a promoter-less beta-galactosidase gene of Leuconostoc mesenteroides resulted in high levels of beta-galactosidase activity in L. lactis. Searching for sequences with similarity to the sequence of the promoter region revealed a consensus sequence of promoters preceeding rRNA operons and tRNA operons from Lactococcus species including a not previously described conserved sequence (AGTT).
Nilsson
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Department of Genetics, Chr. Hansen's Laboratorium, Hørsholm, Denmark.
Johansen
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D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005798:Genes, Bacterial / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000096:biosynthesis / Q000235:genetics*; D012343:RNA, Transfer / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D012341:RNA, Ribosomal, 5S; D011993:Recombinant Fusion Proteins; D012343:RNA, Transfer