Embryonic kidney rudiments grown in adult mice fail to mimic the Wilms' phenotype, but show strain-specific morphogenesis.
12.5- to 14.5-day mouse embryonic kidney fragments were grown syngeneically under kidney or testis capsules in 3 mouse strains for 5-26 weeks to reproduce the Wilms' tumour (WT) phenotype previously reported. About 65% of the transplants grew, but none showed true WT morphology, invaded local tissue or developed metastases. In Swiss mice, most growths contained disorganized stromal and blast cells, but also had more mature structures. CBA growths had cysts, differentiated nephrons and glomeruli, while 129/SV growths gave both types of morphology. In situ mRNA hybridization using the Wilms' tumour predisposition gene (WT1) showed that, unlike the initial rudiments and WT, expression was limited to the glomeruli. The unexpected absence of expression by the apparent blast cells in the growths implies that the system is not a model for WT.
Armstrong
J F
JF
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Kaufman
M H
MH
van Heyningen
V
V
Bard
J B
JB
D000818:Animals; D005260:Female; D016332:Fetal Tissue Transplantation / Q000473:pathology*; D015870:Gene Expression; D016162:Genes, Wilms Tumor; D017403:In Situ Hybridization; D007680:Kidney Neoplasms / Q000209:etiology* / Q000235:genetics; D016030:Kidney Transplantation / Q000473:pathology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010641:Phenotype; D011247:Pregnancy; D013045:Species Specificity; D014185:Transplantation, Isogeneic; D009396:Wilms Tumor / Q000209:etiology* / Q000235:genetics