Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver.
Endogenous DNA adducts may contribute to the etiology of human genetic disease and cancer. One potential source of endogenous DNA adducts is lipid peroxidation, which generates mutagenic carbonyl compounds such as malondialdehyde. A sensitive mass spectrometric method permitted detection and quantitation of the major malondialdehyde-DNA adduct, a pyrimidopurinone derived from deoxyguanosine. DNA from disease-free human liver was found to contain 5400 adducts per cell, a frequency comparable to that of adducts formed by exogenous carcinogens.
Chaudhary
A K
AK
A. B. Hancock Jr. Memorial Laboratory for Cancer Research, Vanderbilt University School of Medicine, Nashville, TN 37232-0146.
Nokubo
M
M
Reddy
G R
GR
Yeola
S N
SN
Morrow
J D
JD
Blair
I A
IA
Marnett
L J
LJ
D000293:Adolescent; D000328:Adult; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015227:Lipid Peroxidation; D008099:Liver / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008315:Malondialdehyde; C089188:3-(2'-deoxyribofuranosyl)pyrimido(1,2-a)purin-10(3H)-one; D004247:DNA; D002251:Carbon Tetrachloride; D003849:Deoxyguanosine