Glutamate uptake system in the presynaptic vesicle: glutamic acid analogs as inhibitors and alternate substrates.
A variety of naturally occurring amino acids, their isomers, and synthetic analogs were tested for their ability to inhibit uptake of [3H]glutamate into presynaptic vesicles from bovine cerebral cortex. Strongest inhibition (Ki < 1mM) was observed for trans-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) and erythro-4-methyl-L-glutamic acid (MGlu), while 4-methylene-L-glutamic acid (MeGlu) was only moderately inhibitory (Ki = approximately 3mM), indicating that the synaptic vesicle glutamate translocator has higher affinity for trans-ACPD and MGlu than for glutamate. A few other amino acids, e.g., 4-hydroxyglutamic acid, S-carboxyethyl cysteine, and 5-fluorotryptophan, were slightly inhibitory; all L- and DL-isomers of protein amino acids and longer chain acidic amino acids were without measurable inhibition. Potassium tetrathionate and S-sulfocysteine exhibited strong to moderate noncompetitive or irreversible inhibition. Inhibition by t-ACPD, MGlu, or MeGlu was competitive with glutamic acid. Each of these competitive inhibitors was also taken up by the vesicle preparation in an ATP-dependent manner, as indicated by their being recovered unchanged from filtered vesicles. Similar results were obtained with reconstituted vesicles, while glutamate uptake by partially purified rat synaptosomes was inhibited only by MGlu. These results indicate that the glutamate translocator of presynaptic vesicles has stringent structural requirements distinct from those of the plasma membrane translocator and the metabotropic type of postsynaptic glutamate receptor. They further suggest possible structural requirements of pharmacologically significant compounds that can substitute for glutamic acid in the presynaptic side of glutamatergic synapses, thus serving to moderate or control glutamate excitation and associated excitotoxic effects in these neurons.
Winter
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HC
Department of Biological Chemistry, Medical School, University of Michigan, Ann Arbor 48109.
Ueda
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D000818:Animals; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism*; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism* / Q000494:pharmacology*; D018698:Glutamic Acid; D013572:Synaptic Vesicles / Q000187:drug effects / Q000378:metabolism*; D014364:Tryptophan / Q000031:analogs & derivatives / Q000494:pharmacology
D005971:Glutamates; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; C046105:4-methylglutamic acid; C026927:5-fluorotryptophan; D018698:Glutamic Acid; C030977:4-methyleneglutamic acid; C011119:S-sulphocysteine; D014364:Tryptophan; D003545:Cysteine