Isolated vestibular sensory cells in the guinea pig.
Vestibular sensory cells were isolated from the utricular macula or cristae ampullares of the guinea pig by enzymatic and mechanical dissociation. The isolated cells were classified into three types: flask-shaped type I sensory cells, rod-shaped type II sensory cells and round-shaped supporting cells. The cilia of type I sensory cells in the crista ampullaris were longer than those in the corresponding cell type in the utricular macula, while no morphological differences of the cell bodies were noted between crista ampullaris and utricular macula. Isolated living vestibular cells have a motile capacity. After exposure to a hypo-osmotic medium, the type I sensory cells showed tilting of the hair bundle. This change in shape may be closely related to the active mechanical transduction control.
Takumida
M
M
Department of Otolaryngology, Hiroshima University School of Medicine, Japan.
Miyawaki
H
H
Arishige
S
S
Harada
Y
Y
D000818:Animals; D002465:Cell Movement; D002469:Cell Separation; D006168:Guinea Pigs; D018069:Hair Cells, Vestibular / Q000166:cytology* / Q000502:physiology