title: Use of DNA fingerprinting for accurate typing of Actinobacillus pleuropneumoniae.
abstract: DNA fingerprinting was tested as a method for detailed differentiation of 23 strains of the causal agent of porcine pleuropneumonia--Actinobacillus pleuropneumoniae. The set consisted of twelve reference strains, each representing one serotype, and eleven field strains belonging to serotype 9 which occurs most frequently in the Czech Republic. Nine of the reference strains could be differentiated from each other and from serotypes 1, 9 and 11, but a very close relatedness was demonstrated among the latter three by repeated examination. Four DNA types were identified among the twelve serotype 9 strains. The findings are discussed within the context of the production and efficiency of industrial and autogenous vaccines.
authors.affiliation: Veterinary Research Institute, Brno.
authors.forename: I
authors.initials: I
authors.lastname: Rychlík
authors.forename: M
authors.initials: M
authors.lastname: Bartos
authors.forename: K
authors.initials: K
authors.lastname: Sesták
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis
chemical_list: D004269:DNA, Bacterial