Biophysical characterization of Vibrio El Tor typing phage e5.
Vibrio cholerae typing phage e5, which can lyse only the El Tor strains of V. cholerae, was characterized. The phage had a polyhedral head 51 nm in diameter and a short tail 13 nm in length. It contained 13 structural polypeptides, with the molecular mass of the major component being 50 kDa. Phage chromosome comprised a 38.5-kb linear double-stranded DNA molecule with unique termini, as determined by restriction fragment analysis and electron microscopy, and had a G+C content of 35.5%. A physical map was constructed with the restriction endonucleases HaeII and HpaII. Adsorption of the phage to its host followed a biphasic kinetics and its intracellular growth was characterized by a latent period of 15 min and a burst size of 100 particles per infected cell. The phage was found to be moderately thermotolerant.
Basu
R
R
Indian Institute of Chemical Biology, Calcutta.
Ghosh
A N
AN
Dasgupta
S
S
Ghosh
A
A
D001435:Bacteriophages / Q000235:genetics* / Q000648:ultrastructure; D003596:Cytosine / Q000032:analysis; D004279:DNA, Viral / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006147:Guanine / Q000032:analysis; D006358:Hot Temperature / Q000009:adverse effects; D008845:Microinjections; D012150:Polymorphism, Restriction Fragment Length; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014734:Vibrio cholerae / Q000145:classification*
D004279:DNA, Viral; D006147:Guanine; D003596:Cytosine