title: Determination of arginase activity in macrophages: a micromethod.
abstract: We propose a modification of Schimke's method for urea determination as a valuable micromethod for measuring arginase in activated macrophages. The method exhibits the following advantages: (a) it uses small amounts of samples (approximately 25,000 macrophages per assay); (b) it does not interfere with other related metabolites that are also present in the activated macrophage such as citrulline or arginine; (c) saturating concentrations of the substrate arginine can be used; and (d) it is much more sensitive than Schimke's method and can detect small amounts of urea, in the order of 0.02 mumol.
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
authors.forename: I M
authors.initials: IM
authors.lastname: Corraliza
authors.forename: M L
authors.initials: ML
authors.lastname: Campo
authors.forename: G
authors.initials: G
authors.lastname: Soler
authors.forename: M
authors.initials: M
authors.lastname: Modolell
mesh_terms: D000818:Animals; D001119:Arginase / Q000032:analysis*; D001854:Bone Marrow Cells; D005260:Female; D008262:Macrophage Activation; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D008832:Microchemistry; D014508:Urea / Q000032:analysis
chemical_list: D014508:Urea; D001119:Arginase