Human peripheral gamma delta T cells recognize hsp60 molecules on Daudi Burkitt's lymphoma cells.
Studies with the use of polyclonal rabbit antiserum reactive with 60-kDa heat shock proteins (hsp60) suggested that hsp60-related molecules could be found on the surface of Daudi cells and were involved in their recognition by certain human gamma delta T cells. The present study confirms this finding by using a mAb specifically recognizing hsp60. This mAb can block outgrowth of human gamma delta T cells in response to stimulation with Daudi and in response to an extract of the mycobacteria H37Ra. This anti-hsp60 mAb stains the surface of Daudi cells, but does not stain either Raji or EBV-transformed B cells, cells which do not stimulate gamma delta T cell outgrowth. Anti-hsp60 mAb could immunoprecipitate a 60-kDa molecule from the H37Ra extract but was unable to precipitate this 60-kDa molecule if the mAb was first absorbed on Daudi cells. This mAb also precipitated a 60-kDa molecule from the surface of Daudi cells which shows an electrophoretic mobility pattern consistent with hsp60. These experiments demonstrate that human gamma delta T cells recognize hsp60-related epitopes on the surface of Daudi cells and within mycobacterial extracts.
Kaur
I
I
Department of Human Oncology, University of Wisconsin, Madison 53792.
Voss
S D
SD
Gupta
R S
RS
Schell
K
K
Fisch
P
P
Sondel
P M
PM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D002051:Burkitt Lymphoma / Q000276:immunology*; D018834:Chaperonin 60; D003429:Cross Reactions; D006360:Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D009161:Mycobacterium / Q000276:immunology*; D011817:Rabbits; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta