Bradykinin elevates tyrosine hydroxylase and dopamine beta-hydroxylase mRNA levels in PC12 cells.
Bradykinin is known to rapidly elevate intracellular calcium leading to secretion of neurotransmitters and short term activation of tyrosine hydroxylase (TH). In this study we examined the effect of bradykinin on mRNA levels of two catecholamine biosynthetic enzymes. Treatment of PC12 cells with 1 microM bradykinin for 3 h markedly elevated both TH and dopamine beta-hydroxylase (DBH) mRNA levels.
Gebreyesus
K
K
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
Kilbourne
E J
EJ
Sabban
E L
EL
D000818:Animals; D015152:Blotting, Northern; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D004299:Dopamine beta-Hydroxylase / Q000096:biosynthesis*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000096:biosynthesis*; D013268:Stimulation, Chemical; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis*
D012333:RNA, Messenger; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D001920:Bradykinin; D002118:Calcium