Separation of large DNA fragments by capillary electrophoresis under pulsed-field conditions.
Capillary zone electrophoresis (CZE), using an entangled polyacrylamide solution, was applied to large DNA samples under pulsed-field conditions. Highly efficient separations were achieved under biased sinusoidal field and field-inversion pulsing regimes. The separations that were obtained with 8.3-48.5 kb lambda DNA standards and 48.5 kb-1 Mb lambda DNA concatamers (modified with ethidium bromide) clearly demonstrate a dramatic improvement in the separation time (roughly, 10-50 times) over the conventionally used slab-gel techniques. Moreover, the CZE method appears much more sensitive and amenable to component quantification and method automation.
Sudor
J
J
Department of Chemistry, Indiana University, Bloomington 47405.
Novotny
M V
MV
D004247:DNA / Q000302:isolation & purification*; D004574:Electromagnetic Fields; D016521:Electrophoresis, Gel, Pulsed-Field; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium / Q000737:chemistry
D004247:DNA; D004996:Ethidium