title: A new polymorphic marker (D10S97) tightly linked to the multiple endocrine neoplasia type 2A (MEN2A) locus.
abstract: Familial multiple endocrine neoplasia type 2A (MEN 2A) is a cancer syndrome that is inherited as an autosomal dominant with high penetrance. Its clinical features are medullary carcinoma of the thyroid, pheochromocytomas, and hyperparathyroidism. A new polymorphic locus D10S97 (probe: KW6 delta SacI) detects a codominant EcoRI polymorphism that is tightly linked to the MEN2A locus. The peak lod score for linkage between D10S97 with MEN2A is 13.03 at theta = 0.00. The polymorphic locus D10S97 maps, by linkage analysis, into the previously defined interval between FNRB and RBP3 to which MEN2A has been assigned. We present physical mapping data showing that the probe pKW6 originates from 10p13 and that the polymorphic locus D10S97 in 10q11.2 is detected by cross-hybridization.
authors.affiliation: Yale University Department of Genetics, New Haven, CT 06510.
authors.forename: J B
authors.initials: JB
authors.lastname: Lichter
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: A R
authors.initials: AR
authors.lastname: Brooks-Wilson
authors.forename: M
authors.initials: M
authors.lastname: Difillipantonio
authors.forename: S
authors.initials: S
authors.lastname: Brewster
authors.forename: D C
authors.initials: DC
authors.lastname: Ward
authors.forename: P J
authors.initials: PJ
authors.lastname: Goodfellow
authors.forename: K K
authors.initials: KK
authors.lastname: Kidd
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D015246:Deoxyribonuclease EcoRI; D005260:Female; D005787:Gene Frequency; D015723:Gene Library; D008040:Genetic Linkage; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008126:Lod Score; D008297:Male; D008969:Molecular Sequence Data; D009377:Multiple Endocrine Neoplasia / Q000235:genetics*; D010375:Pedigree; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA; D015246:Deoxyribonuclease EcoRI