[Intracellular calcium homeostasis. Calmodulin and Ca(2+)-ATPase of the plasma membrane of Trypanosomatids].
The intracellular Ca2+ concentration in all eukaryotic cells so far studied is 4 orders of magnitude lower than in the extracellular milieu. In trypanosomatids, the intracellular concentration of this cation is around 50 nM, even lower than in higher eukaryotics. This differential concentration is maintained by diverse transport systems at the plasma membrane level and at certain intracellular organelles. In the case of trypanosomatids it have been identified the presence of an electrophoretic uniporter at the internal membrane of the unique giant mitochondrion of these parasites, showing identical kinetics properties to the homologous system of higher eukaryotics. Thus, the low Ca2+ affinity of this system is not compatible with its putative function of maintaining intracellular Ca2+ at the submicromolar level. Contrary to previous reports, we have identified a Ca(2+)-ATPase in the plasma membrane of Leishmania braziliensis, Leishmania mexicana, Trypanosoma cruzi and Trypanosoma brucei. The enzyme possesses a high Ca2+ affinity (Km Ca2+ = 0.5 microM), is Mg(2+)-dependent and activatable by calmodulin purified from these hemoflagellates. The Ca(2+)-ATPase is sensitive to vanadate (Ki = 1 microM), thus typifying it as a "P" type ion pump. Vesicles from the plasma membrane of these parasites are able to accumulate Ca2+ against a concentration gradient. The kinetic properties of both transport and ATPase are essentially the same, thus suggesting the same molecular entity. The above results strongly suggest that the Ca(2+)-ATPase is the mechanism responsible for the long-term fine-tuning of intracellular Ca2+ at the submicromolar lever in trypanosomatids.
Benaim
G
G
Centro de Biología Celular, Facultad de Ciencias, Universidad Central de Venezuela, Caracas.
D000818:Animals; D001693:Biological Transport, Active; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism / Q000493:pharmacokinetics*; D002147:Calmodulin / Q000378:metabolism / Q000493:pharmacokinetics*; D002462:Cell Membrane / Q000378:metabolism; D005057:Eukaryotic Cells / Q000378:metabolism / Q000648:ultrastructure; D006706:Homeostasis; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D014351:Trypanosomatina / Q000378:metabolism* / Q000648:ultrastructure
D002147:Calmodulin; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium
Homeostasis intracelular del calcio. La calmodulina y la Ca(2+)-ATPasa de la membrana plasmatica de tripanosomatidios.