title: The ade4 gene of Schizosaccharomyces pombe: cloning, sequence and regulation.
abstract: We report the isolation and sequence of the Schizosaccharomyces pombe ade4 gene which encodes the glutamine phosphoribosylpyrophosphate amidotransferase, the first enzyme of the purine nucleotide de-novo biosynthetic pathway. The enzyme contains 533 amino acids and its sequence exhibits homologies to the corresponding enzymes of Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis, chicken, rat, and human. In contrast to the situation in S. cerevisiae, adenine does not repress ade4 expression at the mRNA level and also other nutritional signals seem not to affect its expression.
authors.affiliation: Institute of General Microbiology, University of Berne, Switzerland.
authors.forename: K M
authors.initials: KM
authors.lastname: Ludin
authors.forename: N
authors.initials: N
authors.lastname: Hilti
authors.forename: M E
authors.initials: ME
authors.lastname: Schweingruber
mesh_terms: D000582:Amidophosphoribosyltransferase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal; D012333:RNA, Messenger; D000582:Amidophosphoribosyltransferase