title: The sequence of an 8.8 kb segment on the left arm of chromosome II from Saccharomyces cerevisiae reveals four new open reading frames including homologs of animal DNA polymerase alpha-primases and bacterial GTP cyclohydrolase II.
abstract: The DNA sequence of two contiguous 7648 bp and 1194 bp BamHI fragments from the cosmid alpha 1201 located about 60 kb from the centromere on the left arm of chromosome II from Saccharomyces cerevisiae has been determined. Sequence analysis reveals four new open reading frames longer than 300 bp: YBL0415 (309 bp), YBL0416 (4539 bp), YBL0417 (1035 bp) and YBL0414 (2115 bp), which extends into the neighbouring 5.2 kb BamHI fragment. The YBL0414 shows homologies to the mouse 68 kDa and Drosophila melanogaster 76 kDa subunits of the DNA polymerase alpha-primase complex. The YBL0417 is homologous to bacterial GTP cyclohydrolase II (EC 3.5.4.25).
authors.affiliation: Unité de Biochemie Physiologique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Skala
authors.forename: L
authors.initials: L
authors.lastname: Van Dyck
authors.forename: B
authors.initials: B
authors.lastname: Purnelle
authors.forename: A
authors.initials: A
authors.lastname: Goffeau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015825:Chromosomes, Fungal; D019915:DNA Primase; D006136:GTP Cyclohydrolase / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D012316:RNA Nucleotidyltransferases / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D006136:GTP Cyclohydrolase