Compartmentalized cytokine production within the human lung in unilateral pneumonia.
The in situ inflammatory response developing in the human lung during a localized bacterial infection was studied in 15 patients with unilateral community-acquired pneumonia (CAP). The local response in the involved lung was compared with that in the contralateral, noninvolved lung as well as with the systemic blood response. Eight healthy volunteers served as control subjects. Concentrations of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and interleukin-6 (IL-6) were measured by ELISA in bronchoalveolar lavage (BAL) fluids (n = 15), serum (n = 15), and alveolar macrophage and monocyte culture supernatants (n = 8). The concentrations of TNF-alpha, IL-beta and IL-6 in BAL fluid were significantly higher in the involved lung than in the paired noninvolved lung (p < or = 0.01) or in healthy subjects (p < or = 0.02, p < or = 0.01, and p < or = 0.001, respectively). Serum IL-6 concentrations were higher in patients than in control subjects, whereas IL-1 beta and TNF-alpha concentrations did not differ in the two groups. Alveolar macrophages from the involved lung spontaneously released higher concentrations of IL-1 beta, IL-6, and TNF-alpha (p < or = 0.05) than did macrophages from the noninvolved lung, which served as controls. However, macrophages were hyporesponsive in terms of cytokine production to further stimulation by lipopolysaccharide (LPS) in the noninvolved and involved lung compared with controls, whereas peripheral blood monocytes were not.(ABSTRACT TRUNCATED AT 250 WORDS)
Dehoux
M S
MS
Inserm U408, Hôpital Bichat, Paris, France.
Boutten
A
A
Ostinelli
J
J
Seta
N
N
Dombret
M C
MC
Crestani
B
B
Deschenes
M
M
Trouillet
J L
JL
Aubier
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D001980:Bronchi / Q000382:microbiology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002478:Cells, Cultured; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000378:metabolism / Q000382:microbiology; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008168:Lung / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry / Q000378:metabolism; D010297:Paris / Q000453:epidemiology; D011014:Pneumonia / Q000453:epidemiology / Q000378:metabolism* / Q000382:microbiology; D011446:Prospective Studies
D016207:Cytokines