Use of capillary electrophoresis-electrospray ionization mass spectrometry in the analysis of synthetic peptides.
We have constructed a capillary electrophoresis (CE) system with UV detection and have successfully interfaced it to an electrospray ionization mass spectrometry (ES-MS) system. A synthesized fragment of heregulin-beta (212-226) was thought to be a single component by re-injection into an HPLC system, but results from CE-UV-ES-MS indicated that a dehydration product was present in the desired peptide sample. A synthetic heregulin-alpha (177-241) was isolated by preparative HPLC, but re-injection on an analytical system indicated a tailing peak. CE-UV-ES-MS indicated a mixture whose two major components were of the same nominal molecular mass (within experimental error), suggesting the presence of an isomer or a deamidation product. The results show that CE-UV-ES-MS can be used as an orthogonal analytical technique to solve practical problems encountered in peptide synthesis laboratories.
Rosnack
K J
KJ
Central Research Division, Pfizer Inc., Groton, CT 06340.
Stroh
J G
JG
Singleton
D H
DH
Guarino
B C
BC
Andrews
G C
GC
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000032:analysis*; D004583:Electrons; D004586:Electrophoresis / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis*; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D020890:Neuregulin-1; D020880:Neuregulins; D010446:Peptide Fragments / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D002352:Carrier Proteins; D006023:Glycoproteins; D007477:Ions; D020890:Neuregulin-1; D020880:Neuregulins; D010446:Peptide Fragments; C089297:heregulin beta (212-226)