title: Direct correlation between cell membrane fluctuations, cell filterability and the metastatic potential of lymphoid cell lines.
abstract: Cell membrane fluctuations, which reflect local bending deformability of the cell surface, are composed of submicron aperiodic reversible displacements of the cell membrane in the frequency range of 0.5-20Hz. Measurements of time-dependent light scattering from small areas (0.25 micron 2) of the cell surface reveal a higher level of cell membrane fluctuations in high metastatic BW5147 T-lymphoma derived cell lines than in their corresponding non-metastatic cell lines. Filterability measurements, carried out by recording the passage time of equal volumes of cell suspensions and media through 8 microns and 10 microns pores of a polycarbonate filter, demonstrate a higher filterability (lower rigidity index) for the high metastatic cell lines than their matched non-metastatic ones. Hence, the present study demonstrates a positive correlation between the metastatic potential of BW5147 lymphoma derived cell lines, their relative level of cell membrane fluctuations and their in-vitro cell filterability.
authors.affiliation: Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
authors.forename: L
authors.initials: L
authors.lastname: Mittelman
authors.forename: S
authors.initials: S
authors.lastname: Levin
authors.forename: H
authors.initials: H
authors.lastname: Verschueren
authors.forename: P
authors.initials: P
authors.lastname: De Baetselier
authors.forename: R
authors.initials: R
authors.lastname: Korenstein
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002462:Cell Membrane / Q000502:physiology*; D005374:Filtration; D006801:Humans; D016399:Lymphoma, T-Cell / Q000473:pathology* / Q000503:physiopathology; D051379:Mice; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D014407:Tumor Cells, Cultured