The enzymes involved in biosynthesis of penicillin and cephalosporin; their structure and function.
The biosynthetic pathway resulting in the penicillins and cephalosporins contains two Fe2+ oxidase enzymes which are responsible for the conversion of alpha-aminoadipoyl-L-cysteinyl-D-valine into isopenicillin N and penicillin N into deacetoxycephalosporin C. We will discuss the studies delineating the ligand binding of these enzymes and present a possible secondary structure.
Cooper
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RD
Lilly Research Laboratories, Eli Lilly & Co., Indianapolis, IN 46285.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002511:Cephalosporins / Q000096:biosynthesis*; D004578:Electron Spin Resonance Spectroscopy; D004798:Enzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D019751:Intramolecular Transferases; D007535:Isomerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000096:biosynthesis*; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D002511:Cephalosporins; D004798:Enzymes; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; C038370:isopenicillin N synthetase; D007535:Isomerases; D019751:Intramolecular Transferases; C038371:deacetoxycephalosporin C synthetase