A neuroprotective effect of histamine H1 receptor antagonist on ischemia-induced decrease in 2-deoxyglucose uptake in rat hippocampal slices.
The effects of histamine (HA) receptor antagonists on hypoxia + hypoglycemia (ischemia)-induced impairment of 2-deoxyglucose (2-DG) uptake by rat hippocampal slices was evaluated. Hippocampal slices were exposed to 20-min ischemia and then returned to oxygenated and glucose-containing Krebs-Ringer solution for 6 h. Ischemia reduced 2-DG uptake in the hippocampal slices. The ischemia-induced reduction in 2-DG uptake was attenuated by pretreatment with H1 receptor antagonist but not with H2 receptor antagonist. Treatment with HA exacerbated the ischemia-induced decrease. The H1 receptor antagonist-induced neuroprotective effect was blocked by co-treatment with HA. The present study suggests that the blockade of H1 receptor-mediated function has a protective role in ischemia-induced decreases in glucose metabolism in hippocampal slices.
Shibata
S
S
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Watanabe
S
S
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002927:Cimetidine / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D011738:Pyrilamine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D002927:Cimetidine; D003847:Deoxyglucose; D011738:Pyrilamine