[Immunochemical identification of kinesin in Physarum polycephalum].
Physarum polycephalum, a low eukaryote ameba provides an attractive system for studying contractile proteins. In this work, we have identified a kinesin-like protein in the plasmodium of Physarum polycephalum by western blotting, using monoclonal antibody against kinesin (bovine brain). The molecular weight of the polypeptide which immunologically cross-reacts with kinesin from bovine brain is about 137kd. It suggests that the 137kd polypeptide is the heavy chain of the kinesin in Physarum polycephalum.
Dong
Z
Z
College of Biological Sciences, Beijing Agricultural University.
Yan
L
L
D000818:Animals; D015153:Blotting, Western; D003429:Cross Reactions; D016547:Kinesins / Q000032:analysis*; D008970:Molecular Weight; D016839:Physarum polycephalum / Q000737:chemistry*
D016547:Kinesins