Tropism for primary monocytes and for monocytoid cell lines are separate features of HIV-1 variants.
We have analyzed the phenotype of five human monocytoid cell lines (U-937, THP-1, RC.2A, Mono Mac 6, and DD) and their capacity to support replication of HIV-1 strains with known tropism for T lymphocytes and mononuclear phagocytes. HIV-1 Ba-L, a prototype monocytotropic HIV-1 strain, was unable to establish continuous replication in any of the cell lines used. In contrast, the lymphotropic strain IIIB could replicate in all CD4-positive cell lines. We conclude that the capacity of HIV-1 isolates to replicate in established human monocytoid malignant cells does not correlate with the tropism of the virus for primary mononuclear phagocytes. Since monocytoid cell lines do not distinguish HIV-1 variants tropic for mononuclear phagocytes, we suggest the use of primary cells for studies of HIV-1 tropism.
Valentin
A
A
Department of Virology, Karolinska Institute, Stockholm, Sweden.
Nilsson
K
K
Asjö
B
B
D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology / Q000503:physiopathology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D016207:Cytokines / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015497:HIV-1 / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007948:Leukemia, Monocytic, Acute / Q000473:pathology; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology; D009000:Monocytes / Q000166:cytology / Q000382:microbiology / Q000502:physiology*; D010586:Phagocytes / Q000166:cytology / Q000382:microbiology / Q000502:physiology; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology / Q000382:microbiology / Q000502:physiology; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D016207:Cytokines