Canine parietal cell binding by antibodies to the complementary peptide of somatostatin.
Using antibodies to a complementary peptide of somatostatin, putative somatostatin binding proteins were characterized on canine parietal cells. A synthetic peptide (S-C1) was derived from the complementary mRNA sequence for somatostatin-14. Antiserum containing antibodies to S-C1 inhibited competitively 125I-Tyr11-somatostatin binding to canine oxyntic mucosal membranes. Canine parietal cell preparations were incubated with carbachol in the presence or absence of somatostatin and antisera to S-C1. Antibodies to S-C1 produced a decrease in carbachol-stimulated 14C-aminopyrine uptake comparable with that produced by 10(-6) M somatostatin. In immunocytochemical studies by light microscopy, antibodies to S-C1 produced positive staining of parietal cells throughout the oxyntic gland area. By electron microscopy using immunogold techniques, binding by antibodies to somatostatin C-1 was localized ultrastructurally to basolateral and intracellular membranes and to secretory canalicular membranes of parietal cells. These studies support the conclusion that antibodies to the somatostatin complementary peptide demonstrate properties similar to those of somatostatin in that they inhibit carbachol-stimulated aminopyrine uptake and 125I-somatostatin binding. Furthermore, these antibodies localize to specific regions on plasma membranes of parietal cells, which may represent somatostatin binding sites.
Campbell-Thompson
M
M
Department of Medicine, College of Medicine, University of Florida, Gainesville 32610.
McGuigan
J E
JE
D000595:Amino Acid Sequence; D000632:Aminopyrine / Q000493:pharmacokinetics; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001483:Base Sequence; D004285:Dogs; D005260:Female; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010295:Parietal Cells, Gastric / Q000737:chemistry*; D011817:Rabbits; D017481:Receptors, Somatostatin / Q000032:analysis*; D013004:Somatostatin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D017481:Receptors, Somatostatin; D000632:Aminopyrine; D013004:Somatostatin