Pharmacokinetic fate and pathological effects of 14C-fumonisin B1 in laying hens.
Presence of fumonisin B1 (FB1), a major metabolite of Fusarium moniliforme, in corn is of great concern to both human and animal health because of its wide range of toxicity. The pharmacokinetics of FB1 was studied in laying hens following oral and intravenous administration of 14C-labelled FB1. After iv dosing (2.0 mg = 23.68 kBq/kg bw) plasma radioactivity underwent a very rapid bi-exponential decline (t1/2 alpha = 2.5 +/- 0.3 min; t1/2 beta = 48.8 +/- 11.2 min) with negligible levels measured after 4-6 hr. Mean value for the apparent volume of distribution at steady state (Vdss) was 18.27 ml/kg, apparent volume of central compartment (Vd beta) was 82.20 ml/kg and plasma clearance was 1.18 ml/min/kg. At 24 hr post-dosing only trace residues were present in liver, kidney, and cecum. When dosed by the oral route (2.0 mg = 47.36 kBq/kg bw), systemic absorption of fumonisin appeared to be poor (F = 0.71 +/- 0.5%) with peak plasma concentrations of only 40-145 dpm/ml (equivalent to 28-103 ng FB1 and/or metabolites per ml) between 1.5 and 2.5 hr. At 24 hr post-dosing only trace amounts were present in crop, liver, kidney, small intestine, and cecum. In both orally and iv dosed birds almost all (97.7 +/- 3.73%) of the radioactivity was recovered in excreta by the end of the 24 hr experiment period and no residues were found in eggs laid during the 24 hr post-dosing period.(ABSTRACT TRUNCATED AT 250 WORDS)
Vudathala
D K
DK
Centre for Food and Animal Research, Agriculture Canada, Central Experimental Farm, Ottawa, Ontario.
Prelusky
D B
DB
Ayroud
M
M
Trenholm
H L
HL
Miller
J D
JD
D000284:Administration, Oral; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002274:Carcinogens, Environmental / Q000493:pharmacokinetics* / Q000633:toxicity*; D002645:Chickens / Q000378:metabolism*; D005260:Female; D037341:Fumonisins; D007275:Injections, Intravenous / Q000662:veterinary; D007408:Intestinal Absorption; D009183:Mycotoxins / Q000097:blood / Q000493:pharmacokinetics* / Q000633:toxicity*; D011485:Protein Binding; D014018:Tissue Distribution
D001798:Blood Proteins; D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1