title: Characterization of two crystal forms of Clostridium thermocellum endoglucanase CelC.
abstract: Endoglucanase CelC from Clostridium thermocellum expressed in Escherichia coli has been crystallized in two different crystal forms by the hanging drop method. Crystals of form I were grown with polyethylene glycol as a precipitant. They are orthorhombic, space group P2(1)2(1)2(1), with cell dimensions a = 51.4 A, b = 84.3 A, and c = 87.5 A. Crystals of form II, obtained in ammonium sulfate solutions, belong to the tetragonal space group P4(1)2(1)2 (or P4(3)2(1)2) with cell dimensions of a = b = 130.7 A and c = 69.6 A. Diffraction data to 2.8 A resolution were observed for both crystal forms with a rotating anode generator. Preliminary oscillation images of the orthorhombic form I crystals using a synchrotron radiation source show diffraction to 2.2 A resolution, indicating that these crystals are suitable for high resolution crystallographic analysis.
authors.affiliation: Unité d'Immunologie Structurale, URA 359 CNRS, Institut Pasteur, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Dominguez
authors.forename: H
authors.initials: H
authors.lastname: Souchon
authors.forename: P M
authors.initials: PM
authors.lastname: Alzari
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D003013:Clostridium / Q000201:enzymology*; D018360:Crystallography, X-Ray
chemical_list: C078899:endoglucanase CelC; D002480:Cellulase