Transcription, translation, and cellular localization of PDV-E66: a structural protein of the PDV envelope of Autographa californica nuclear polyhedrosis virus.
A late gene encoding a 66-kDa structural protein of Autographa californica nuclear polyhedrosis virus was mapped and sequenced (26.9-29.7 MU). Transcription initiates from two conserved TAAG motifs (-15 and -37) with transcripts detected from 12 to 72 hr pi. The protein is detected in infected Spodoptera frugiperda (Sf9) cells from 24 to 72 hr pi. Western-blot and immunoelectron microscopic data identify this protein as specific for the polyhedra-derived virus (PDV) envelope. This protein has been named PDV-E66 to identify its viral origin, envelope location, and apparent molecular weight. In addition to being a structural protein of the PDV envelope, PDV-E66 is enriched in foci of microvesicles in the nuclei of infected Sf9 cells.
Hong
T
T
Department of Biology, Texas A & M University, College Station 77843.
Braunagel
S C
SC
Summers
M D
MD
D000595:Amino Acid Sequence; D001483:Base Sequence; D005814:Genes, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000382:microbiology / Q000648:ultrastructure; D017924:Nucleopolyhedroviruses / Q000737:chemistry* / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D014771:Virion / Q000737:chemistry* / Q000648:ultrastructure
C089454:Ac-odv-e66 protein, Autographa californica nucleopolyhedrovirus; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins