The immunodominant peptide from listeriolysin in Quil A liposomes vaccinates CD8+ cytolytic T cells and confers protection to infection.
Cytolytic T-cell vaccination with immunodominant MHC class I-restricted peptides contained within Quil A liposomes has been previously demonstrated. In recent years, Quil A has been under consideration for use as an adjuvant in humans. We assessed the possible use of peptide inoculation in the context of Quil A liposomes to be protective in a mouse model. Listeria monocytogenes was used as the challenge pathogen and the previously identified listeriolysin 91-99 peptide as the immunogen. The listeriolysin 91-99 Quil A liposome inoculum showed significant enhancement of survival after challenge with up to 10 times the L. monocytogenes LD50.
Lipford
G B
GB
Institute for Medical Microbiology, Technical University of Munich, Germany.
Lehn
N
N
Bauer
S
S
Heeg
K
K
Wagner
H
H
D000276:Adjuvants, Immunologic; D000818:Animals; D001427:Bacterial Toxins; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D016827:CD8 Antigens; D003602:Cytotoxicity, Immunologic; D005260:Female; D006360:Heat-Shock Proteins / Q000276:immunology*; D006460:Hemolysin Proteins / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000276:immunology*; D008081:Liposomes; D008088:Listeriosis / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000276:immunology; D062605:Quillaja Saponins; D012503:Saponins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D016827:CD8 Antigens; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D016056:Immunodominant Epitopes; D008081:Liposomes; D009842:Oligopeptides; D062605:Quillaja Saponins; D012503:Saponins; C046386:Quil A; C038705:hlyA protein, Listeria monocytogenes