Nearest neighbor analysis of D1 and D2 subunits in the photosystem II reaction center using a bifunctional cross-linker, hexamethylene diisocyanate.
A cross-linked product between the D1 and D2 subunits was generated by treating isolated spinach PS II reaction center with a bifunctional cross-linker, 1,6-hexamethylene diisocyanate. To obtain information on the contact site(s) between D1 and D2 proteins, the cross-linked product was cleaved by CNBr, the resultant fragments were separated using a reverse-phase HPLC, and then the partial sequence of the cross-linked polypeptide fragments were determined. By comparing the results with the deduced amino acid sequence of the D1 and D2 proteins from spinach, it is concluded that the C-terminal domains of the D1 subunit (D308-A334) and that of the D2 subunit (Y297-L353) are in close proximity.
Tomo
T
T
Department of Biology, Okayama University, Japan.
Satoh
K
K
D000595:Amino Acid Sequence; D003432:Cross-Linking Reagents; D003485:Cyanates / Q000737:chemistry*; D017953:Isocyanates; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D045332:Photosystem II Protein Complex
D003432:Cross-Linking Reagents; D003485:Cyanates; D017953:Isocyanates; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C015262:1,6-hexamethylene diisocyanate