An agar-overlay method for detection of toxins produced by Escherichia coli.
An agar overlay method, with Vero and HeLa cells, was used for detection of heat-labile enterotoxin and verotoxin from Escherichia coli. The method is more sensitive than the conventional cell culture assay, is rapid, easy to perform, and is suitable for epidemiological studies.
Parreira
V R
VR
Departamento de Microbiologia e Imunologia, Universidade Estadual de Campinas, São Paulo, Brazil.
Arns
C W
CW
Yano
T
T
D000362:Agar; D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis*; D001431:Bacteriological Techniques; D004768:Enterotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006367:HeLa Cells; D006801:Humans; D012680:Sensitivity and Specificity; D022622:Shiga Toxin 1; D014709:Vero Cells
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022622:Shiga Toxin 1; D000362:Agar; C027538:heat-labile enterotoxin, E coli