The antifertility agent, gossypol, releases calcium from rat liver mitochondria.
1. The effect of gossypol in the presence of K+ or Ca2+, or both, was studied on respiration, ATPase activity, and Ca2+ transport of rat liver mitochondria. 2. The uncoupled respiration induced by gossypol was inhibited by Ca2+. 3. This inhibition was lowered by the presence of Mg2+. ATPase activity was stimulated when gossypol and Ca2+ were in the incubation medium, in a Ca(2+)-dose related fashion. 4. Mitochondrial Ca2+ was released by gossypol, an effect associated with the membrane fluidity. 5. The results suggest that gossypol modifies the mitochondrial concentration of Ca2+.
Martínez
F
F
Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, D.F.
Milán
R
R
Espinosa-García
T
T
Pardo
J P
JP
D000251:Adenosine Triphosphatases / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D005298:Fertility / Q000187:drug effects*; D006072:Gossypol / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats
D000251:Adenosine Triphosphatases; D006072:Gossypol; D002118:Calcium