A potassium channel toxin from the secretion of the sea anemone Bunodosoma granulifera. Isolation, amino acid sequence and biological activity.
A peptide toxin affecting potassium channels was isolated from the sea anemone Bunodosoma granulifera. It facilitates acetylcholine release at avian neuromuscular junctions, competes with dendrotoxin I, a probe for voltage-dependent potassium channels, for binding to synaptosomal membranes of rat brain with a Ki of 0.7 nM and suppresses K+ currents in rat dorsal root ganglion neurones in culture. It represents a new structural type of potassium channel toxin with the sequence V1RCDWFKETA10CRHAKSLGNC20RTSQKYRANC30AKTLQCC37 (M(r) 4275, three disulfides).
Aneiros
A
A
Instituto de Investigaciones Fundamentales del Cerebro, La Habana, Cuba.
García
I
I
Martínez
J R
JR
Harvey
A L
AL
Anderson
A J
AJ
Marshall
D L
DL
Engström
A
A
Hellman
U
U
Karlsson
E
E
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D002850:Chromatography, Gel; D003063:Cnidaria; D003064:Cnidarian Venoms / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009469:Neuromuscular Junction / Q000187:drug effects; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013570:Synaptic Membranes / Q000187:drug effects
D003064:Cnidarian Venoms; D015221:Potassium Channels