Comparative metabolism of 3H-glucosamine by fibroblast populations exposed to cyclosporine.
Cyclosporine and nifedipine therapy produces gingival overgrowth in many patients. Neither the mechanism underlying this undesirable side effect nor the possibility of synergism between these drugs is known, although many renal transplant patients receive both drugs. This study compared the rates of 3H-glucosamine utilization by three groups of fibroblasts: untreated gingival fibroblasts, fibroblasts from gingival overgrowth tissue of a patient receiving both cyclosporine and nifedipine, and normal gingival fibroblasts exposed to cyclosporine-A in vitro. Significant differences in the rates of deposition of 3H-glucosamine into the extracellular matrix by each group of gingival fibroblasts were demonstrated, suggesting that increased rates of deposition of proteoglycans into the gingival extracellular matrix by fibroblasts should be further investigated as a biologic mechanism for gingival overgrowth.
Zebrowski
E J
EJ
Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg.
Pylypas
S P
SP
Odlum
O
O
Johnson
R B
RB
D000328:Adult; D002452:Cell Count; D002478:Cells, Cultured; D002908:Chronic Disease; D016572:Cyclosporine / Q000009:adverse effects / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D005885:Gingival Hyperplasia / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D005944:Glucosamine / Q000378:metabolism*; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D009543:Nifedipine / Q000009:adverse effects / Q000494:pharmacology; D011509:Proteoglycans / Q000378:metabolism; D014316:Tritium
D011509:Proteoglycans; D014316:Tritium; D016572:Cyclosporine; D009543:Nifedipine; D005944:Glucosamine