Purification of a sea urchin calpactin like protein which is excluded from the mitotic apparatus region.
A calpactin like protein (SUAX34) was purified from fertilized sea urchin eggs. Ca2+ dependent binding to and EGTA induced elution from a phenyl column were used to detect and isolate the calpactin like protein. SUAX34 showed monomeric nature having a molecular mass of 34,000. Immunoblotting of the cell extract showed that closely positioned two bands were reactive to the antibody against SUAX34 and immunostaining experiments showed specific exclusion of these proteins from the mitotic apparatus of metaphase eggs.
Maekawa
S
S
Graduate School, Division of Science, University of Tokyo, Japan.
Toriyama
M
M
Sakai
H
H
D000818:Animals; D017302:Annexins / Q000737:chemistry / Q000302:isolation & purification*; D002118:Calcium / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005306:Fertilization; D015151:Immunoblotting; D008081:Liposomes / Q000378:metabolism; D008970:Molecular Weight; D010063:Ovum / Q000737:chemistry*; D011247:Pregnancy; D011485:Protein Binding; D012617:Sea Urchins
D017302:Annexins; D008081:Liposomes; D002118:Calcium