Selective sub-dissection of the striatal primordium for cultures affects the yield of DARPP-32-containing neurones.
We have studied the effect of selectively dissecting the lateral (LGE) and medial (MGE) ganglionic eminences of the striatal primordium on the yield of DARPP-32-immunoreactive striatal neurones in serum-free cultures maintained for 2 or 5 days. The LGE cultures yielded an approximately four-fold higher number of DARPP-32-positive neurones compared with the MGE cultures, without an accompanying difference in total cell numbers. In both types of culture, there was a 70% reduction in DARPP-32 positive neurones from 2 to 5 days in vitro. In conclusion, the LGE is a rich source of striatal precursors and the present culture system provides a good model for the study of factors that may prevent striatal neuronal death.
Nakao
N
N
Department of Neurology, University Hospital, Lund, Sweden.
Odin
P
P
Brundin
P
P
D000818:Animals; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D007150:Immunohistochemistry; D008473:Median Eminence / Q000166:cytology / Q000378:metabolism; D017072:Neostriatum / Q000166:cytology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000276:immunology; D009474:Neurons / Q000378:metabolism*; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000187:drug effects / Q000378:metabolism
D016895:Culture Media, Serum-Free; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D017447:Receptors, Dopamine D1