Effect of perfusion of oral rehydration solutions containing glucose polymers from corn on disaccharidases and mucosal morphology in rat small intestines.
This study aims to determine the effect of glucose and glucose polymers (GP) from corn in oral rehydration solutions (ORS) on disaccharidases and morphometric measurements in small intestinal mucosa of rats. ORS containing standard composition of salts as in WHO ORS and 2, 5, or 10 per cent glucose or GP [initial glucose polymers, long chain (> 9 molecules) and short chain (2-9 molecules) glucose polymers] from corn were infused into the duodenum of 72 Sprague-Dawley rats (250-350 g). Six rats were sham operated as controls. The levels of lactase, sucrase, maltase, palatinase, and glucoamylase enzymes were higher in rats infused with ORS-containing glucose or GP than control rats. Villus height, villus width, and crypt height in corresponding segments of duodenum, jejunum, and ileum were not significantly different between rats perfused with ORS containing glucose polymers from corn and those with ORS containing glucose. ORS containing GP from corn have no adverse effects on small intestinal enzymes and morphometric measurements.
Zheng
B Y
BY
Department of Pediatrics, Hahnemann University, Philadelphia, PA 19102-1192.
Khin-Maung-U
Lu
R B
RB
Maiese
R L
RL
Lebenthal
E
E
D000818:Animals; D004186:Disaccharidases / Q000187:drug effects; D005440:Fluid Therapy; D005936:Glucans / Q000008:administration & dosage*; D005947:Glucose / Q000008:administration & dosage*; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology; D007421:Intestine, Small / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology; D043322:Lactase; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015532:Rehydration Solutions / Q000008:administration & dosage*; D013393:Sucrase / Q000187:drug effects; D003313:Zea mays; D000520:alpha-Glucosidases / Q000187:drug effects; D001616:beta-Galactosidase / Q000187:drug effects
D005936:Glucans; D015532:Rehydration Solutions; D004186:Disaccharidases; C022310:palatinase; D043322:Lactase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D013393:Sucrase; D005947:Glucose