[Gas chromatographic method for determining esterase activity of proteolytic enzymes using continuous gas extraction of the volatile product].
A new procedure was developed for estimating the esterase activity of proteolytic enzymes by using CIS subcomponent of complement and N-alpha-benzoyl-L-arginine ethyl ester as a substrate. In contrast to other methods, which used static variants of gas chromatographic vapour-phase analysis for registration of the volatile reaction product alcohol, the procedure involved steady gas extraction of the volatile product in the gas stream over the incubation mixture. This method enabled to obtain a kinetic curve during one experiment as well as to elevate the accuracy of enzymatic activity estimation.
Bureĭko
A S
AS
Dorofeĭkov
V V
VV
Shcherbak
I G
IG
D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D002849:Chromatography, Gas; D010450:Endopeptidases / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism*; D007700:Kinetics; D013379:Substrate Specificity; D014835:Volatilization
D001120:Arginine; C009727:benzoylarginine ethyl ester; D004950:Esterases; D010450:Endopeptidases
Gazokhromatograficheskiĭ sposob opredeleniia ésteraznoĭ aktivnosti proteoliticheskikh fermentov s pomoshch'iu nepreryvnoĭ gazovoĭ ékstraktsii letuchego podukta.