title: Cloning and sequencing of the cDNAs encoding two alternative splicing-derived variants of the alpha subunit of the granulocyte-macrophage colony-stimulating factor receptor.
abstract: Two distinct cDNA clones, corresponding to alternative mRNA splicing variants of the alpha subunit of the granulocyte-macrophage colony-stimulating factor receptor (GM-CSF-R alpha) were isolated from human blood mononuclear cells by RT-PCR. The first one lacks 179 bp, and the second one lacks 136 bp of the major variant, GM-CSF-R alpha 1. Both of variants result in shifts in the reading frame and are expected to encode 377- and 285-amino-acid membrane anchoring and soluble receptor isoforms, respectively.
authors.affiliation: Division of Medical Oncology and Hematology, University of South Florida, Tampa 33612.
authors.forename: X
authors.initials: X
authors.lastname: Hu
authors.forename: P D
authors.initials: PD
authors.lastname: Emanuel
authors.forename: K S
authors.initials: KS
authors.lastname: Zuckerman
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D012326:RNA Splicing / Q000235:genetics; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor