Association of GPI-anchored glycoproteins with other components of the leucocyte membrane.
The leucocyte surface glycosylphosphatidylinositol (GPI)-anchored membrane proteins are localized within specific membrane microdomains which also contain specific (glyco)lipids and intracellular proteins including protein kinases. These "GPI-domains" are devoid of most abundant transmembrane proteins, but in T-cells they appear to contain small amounts of CD4 and CD8 and in B-cell lines, small amounts of CD10. The existence of these relatively detergent-resistant membrane microdomains explains the signal-transducing ability of GPI-anchored receptors. In addition to the "GPI-microdomains", several other types of analogous very large detergent-resistant complexes/domains appear to exist, such as those containing T-cell receptor, others containing CD45R molecules associated with a protein kinase, and still others composed mainly of several proteins of the tetraspan family. Therefore, we suggest that the leucocyte surface is a mosaic of microdomains of unique composition associated with specific signal-transducing molecules.
Horejsí
V
V
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague.
Cinek
T
T
Angelisová
P
P
Cerný
J
J
Hilgert
I
I
D015703:Antigens, CD / Q000032:analysis* / Q000737:chemistry; D000954:Antigens, Surface / Q000032:analysis* / Q000737:chemistry; D001402:B-Lymphocytes / Q000737:chemistry / Q000276:immunology; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis / Q000737:chemistry*; D006801:Humans; D007962:Leukocytes / Q000737:chemistry* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000737:chemistry; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology
D015703:Antigens, CD; D000954:Antigens, Surface; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins