Validation of the species concept in the genus Dekkera by restriction analysis of genes coding for rRNA.
The gene coding for the small-subunit rRNA of 11 type strains belonging to the genus Dekkera and its anamorph, Brettanomyces, was amplified by using the polymerase chain reaction and subjected to digestion with a series of restriction endonucleases. Similarity coefficients were calculated from the number of shared and unique fragments, and a cluster analysis yielded four distinct groups with the following ascosporogenous states: Dekkera anomala, Dekkera bruxellensis, Dekkera custersiana, and Dekkera naardenensis. Results correlate with evidence from isoenzyme electrophoresis and DNA homology analysis. They also confirm previously reported anamorph-teleomorph connections and recently proposed synonymies within the genus.
Molina
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American Type Culture Collection, Rockville, Maryland 20852.
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Jong
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D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D015003:Yeasts / Q000145:classification* / Q000235:genetics*
D004271:DNA, Fungal; D012335:RNA, Ribosomal