Binding of bacterial adhesins to rat glomerular mesangium in vivo.
Two well characterized bacterial adhesins, the O75X fimbriae of Escherichia coli and the type-3 fimbriae of Klebsiellae, with in vitro affinities to type IV and V collagens, respectively, were used to test whether bacterial components with affinity for glomerular matrix could bind to glomeruli in vivo. The purified fimbrial proteins were injected into rats, and kidney samples were studied by immunofluorescence at two hours to nine months postinjection. The O75X, but not the type-3 fimbriae, formed mesangial deposits that persisted for months. Preincubation of the O75X fimbriae with type IV collagen significantly reduced the glomerular binding. The fimbrial deposits were extracellular, as anti-O75X IgG injected into rats bound to glomeruli. Proteinuria or histological damage could not be detected even after passive or active immunizations of the rats. The results demonstrate that bacterial adhesins may bind in vivo to and persist in glomeruli by their specific affinities. The results also indicate that additional factors provided by the bacteria or the host are needed for glomerular damage to take place.
Miettinen
A
A
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Westerlund
B
B
Tarkkanen
A M
AM
Törnroth
T
T
Ljungberg
P
P
Renkonen
O V
OV
Korhonen
T K
TK
D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism / Q000382:microbiology; D005260:Female; D036781:Fimbriae Proteins; D005455:Fluorescent Antibody Technique; D005920:Glomerular Mesangium / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000378:metabolism; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley
D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C058768:Klebsiella pneumoniae type 3 fimbrial adhesin; D036781:Fimbriae Proteins; D003094:Collagen