title: Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex.
abstract: We show here that the state of the p34cdc2-p56cdc13 mitotic B cyclin complex determines whether a fission yeast cell undergoes S phase or mitosis. Mutants defective for p56cdc13 reset to G1 and rereplicate their DNA, while cells completely lacking the p34cdc2-p56cdc13 complex undergo multiple rounds of S phase. In contrast, formation of the p34cdc2-p56cdc13 complex in G1 promotes cells inappropriately into mitosis. We propose that the temporal order of S phase and mitosis is maintained by the presence or absence of the p34cdc2-p56cdc13 complex.
authors.affiliation: Cell Cycle Laboratory, Imperial Cancer Research Fund, London, England.
authors.forename: J
authors.initials: J
authors.lastname: Hayles
authors.forename: D
authors.initials: D
authors.lastname: Fisher
authors.forename: A
authors.initials: A
authors.lastname: Woollard
authors.forename: P
authors.initials: P
authors.lastname: Nurse
mesh_terms: D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism*; D002451:Cell Compartmentation; D019926:Cyclin B; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication / Q000502:physiology; D016193:G1 Phase / Q000502:physiology; D008938:Mitosis / Q000235:genetics / Q000502:physiology*; D008954:Models, Biological; D009154:Mutation; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D016196:S Phase / Q000235:genetics / Q000502:physiology*; D012568:Schizosaccharomyces / Q000235:genetics / Q000254:growth & development*; D013997:Time Factors
chemical_list: D019926:Cyclin B; D016213:Cyclins; D011994:Recombinant Proteins; D016203:CDC2 Protein Kinase