Arachidonic acid and eicosanoid release.
The major techniques for the study and determination of eicosanoid metabolism in eukaryotic cells will be described. These techniques include the determination of free arachidonic acid and its metabolites in biological fluids and cell media by immunochemical techniques (RIA, ELISA) and by HPLC. The different methods will be explained and critically compared.
Dieter
P
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University of Freiburg, Biochemical Institute, Tumor Biology Center, Germany.
D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis* / Q000378:metabolism; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D015777:Eicosanoids / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000379:methods; D066298:In Vitro Techniques
D003470:Culture Media; D015777:Eicosanoids; D016718:Arachidonic Acid