Histochemical evidence for abnormal copper distribution in the central nervous system of LEC mutant rat.
After sulfide-silver staining if tissue sections treated with trichloroacetic acid, silver particles indicating the copper distribution were observed in several regions of normal rat brain, including the nucleus arcuatus, nucleus tractus solitarii, nucleus habenulae medialis, locus ceruleus, and cerebellum. However, copper in these regions disappeared or was greatly decreased in LEC mutant rats. The are postrema showed no detectable level of copper in normal rat brain, but exhibited an extremely high level of copper in LEC rats, with or without the onset of jaundice. The results suggest an abnormal copper metabolism in the central nervous system of LEC rat.
Sato
M
M
Department of Anatomy, Akita University School of Medicine, Japan.
Sugiyama
T
T
Daimon
T
T
Iijima
K
K
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002570:Ceruloplasmin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D015870:Gene Expression; D006520:Hepatitis, Animal / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006651:Histocytochemistry; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D016622:Silver Staining
D003300:Copper; D002570:Ceruloplasmin