Measurements of urinary adipic acid and suberic acid using high-performance liquid chromatography.
A sensitive and specific method was developed for measuring medium-chain dicarboxylic acids (adipic and suberic acid) in urine. These acids were extracted from urine with diethyl ether and converted into fluorescent derivatives with 9-anthryldiazomethane, which can be separated by high-performance liquid chromatography. The reproducibility was high and the recovery from urine was above 90%. Urinary concentrations of adipic acid in streptozotocin-induced diabetic rats were significantly higher than those in control rats. In diabetic patients, both adipic acid and suberic acid tended to be high, but not significantly. This method should be useful for measuring dicarboxylic acids in urine.
Yoshioka
K
K
Department of Laboratory Medicine, Osaka City University Medical School, Japan.
Shimojo
N
N
Nakanishi
T
T
Naka
K
K
Okuda
K
K
D000272:Adipates / Q000652:urine*; D000818:Animals; D002210:Caprylates; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D003920:Diabetes Mellitus / Q000652:urine; D003921:Diabetes Mellitus, Experimental / Q000652:urine; D003998:Dicarboxylic Acids / Q000652:urine*; D006801:Humans; D051381:Rats; D015203:Reproducibility of Results
D000272:Adipates; D002210:Caprylates; D003998:Dicarboxylic Acids; C005738:suberic acid; C029900:adipic acid