title: IFN-gamma influences the migration of thoracic duct B and T lymphocyte subsets in vivo. Random increase in disappearance from the blood and differential decrease in reappearance in the lymph.
abstract: Thoracic duct lymphocytes (TDL) continuously patrol through the body, facilitating immune responses at most sites. IFN-gamma might regulate immune responses by influencing the migration of TDL. Therefore, it was investigated in vivo whether IFN-gamma affects the migration of thoracic duct B, T, CD4+, and CD8+ lymphocytes from blood to lymph. Labeled TDL were injected i.v. into rats continuously receiving IFN-gamma via a central venous catheter. The numbers of B, T, CD4+, and CD8+ lymphocytes were determined in blood and thoracic duct lymph for 120 h. IFN-gamma increased the disappearance of TDL from the blood to a similar extent in all subsets. In contrast, the reappearance of B and T lymphocyte subsets in the lymph was decreased: B lymphocytes were affected significantly more than T lymphocytes, whereas CD4+ and CD8+ lymphocytes were affected to a similar extent. Our study suggests that differential retention within the tissue rather than preferential immigration into the tissue creates a microenvironment with a distinct composition of lymphocyte subsets.
authors.affiliation: Center of Anatomy, Medical School of Hannover, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Westermann
authors.forename: S
authors.initials: S
authors.lastname: Persin
authors.forename: J
authors.initials: J
authors.lastname: Matyas
authors.forename: P
authors.initials: P
authors.lastname: van der Meide
authors.forename: R
authors.initials: R
authors.lastname: Pabst
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000187:drug effects* / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000502:physiology; D016827:CD8 Antigens / Q000032:analysis; D002465:Cell Movement / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology*; D007958:Leukocyte Count; D008196:Lymph / Q000166:cytology*; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000502:physiology; D013897:Thoracic Duct / Q000166:cytology*
chemical_list: D016827:CD8 Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma