Free D-serine, D-aspartate and D-alanine in central nervous system and serum in mutant mice lacking D-amino acid oxidase.
We have examined whether D-amino acid oxidase (DAO) regulates free D-serine content using mutant ddY/DAO- mice lacking DAO activity. We find that the content of D-serine in the serum and cerebellum of mutant mice is much higher than that of normal mice, whereas a slight but significant difference in the cerebral D-serine level is observed between the two strains. These results suggest that, although DAO may participate in the catabolism of D-serine in the cerebellum and periphery, there appears to be other mechanisms for catabolism of endogenous D-serine in the brain.
Hashimoto
A
A
Group of Precursory Research for Embryonic Science and Technology, Research Development Corporation of Japan, Tokyo.
Nishikawa
T
T
Konno
R
R
Niwa
A
A
Yasumura
Y
Y
Oka
T
T
Takahashi
K
K
D000409:Alanine / Q000032:analysis* / Q000097:blood; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis* / Q000097:blood; D001923:Brain Chemistry; D002531:Cerebellum / Q000737:chemistry; D003605:D-Amino-Acid Oxidase / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains / Q000378:metabolism*; D012694:Serine / Q000032:analysis* / Q000097:blood; D013237:Stereoisomerism
D001224:Aspartic Acid; D012694:Serine; D003605:D-Amino-Acid Oxidase; D000409:Alanine