Flow cytometric analysis of the direct toxic effects of paraquat on cultured MDCK cells.
We used flow cytometry to assess the cell cycle kinetics of cultured Maden Darby canine kidney cells after exposure to paraquat. Fluorescein diacetate fluorescence was used as a marker of cell viability, while bromodeoxyuridine incorporation was detected with a monoclonal antibody and propidium iodide staining to assess DNA synthesis. Flow cytometry was performed immediately, 48 h and 96 h after exposure to paraquat for 24 h. Lactate dehydrogenase release was also measured to determine the extent of cytolysis. Flow cytometry of paraquat-treated cells showed a marked increase of the S phase population immediately after exposure, at a time when there was no increase of lactate dehydrogenase release. In contrast, the cell cycle profile returned towards normal at 48 and 96 h after paraquat exposure, but lactate dehydrogenase release increased. These findings indicate that paraquat arrested cells in the S phase and that inhibition of DNA synthesis by this agent appeared to influence cell viability because S phase block occurred before cytolysis. In addition, this method proved useful for assessing the effects of paraquat on DNA synthesis by cultured cells.
Yamagami
K
K
Department of Emergency and Critical Care Medicine, Kansai Medical University, Osaka, Japan.
Matsubara
M
M
Kitazawa
Y
Y
Takeyama
N
N
Tanaka
T
T
Kawamoto
K
K
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D004285:Dogs; D005434:Flow Cytometry / Q000379:methods; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D008297:Male; D010269:Paraquat / Q000633:toxicity*
D004247:DNA; D007770:L-Lactate Dehydrogenase; D010269:Paraquat