title: [Proteolytic processing by dipeptidyl aminopeptidase IV generates receptor selectivity for peptide YY (PYY)].
abstract: Two receptor subtypes, Y1 and Y2, are known to mediate PYY biological activity. PYY 1-36 binds to Y1 and Y2 receptors with equal affinity, whereas the second endogenous form of PYY, PYY 3-36, selectively binds to Y2 receptors. Dipeptidyl cleavage thus transforms an unselective Y agonist into a highly selective Y2 agonist, PYY 3-36. The enzyme responsible for this processing is unknown. Since PYY has a proline in the penultimate position it is protected from the attack of most unspecific exopeptidases. Only a few exopeptidases are theoretically capable of generating PYY 3-36 from PYY 1-36. Of the enzymes tested only the dipeptidyl aminopeptidase IV (DPP IV, E.C. 3.4.14.5) cleaved Tyr-Pro from PYY 1-36 with high activity. Since DPP IV is found on the endothelial surface and brush border membranes it can be considered a candidate enzyme for generating PYY 3-36 in vivo, thereby regulating the ratio of Y1/Y2 receptor stimulation by PYY.
authors.affiliation: Medizinische Klinik und Poliklinik, Abteilung für Gastroenterologie, Klinikum der Universität, Essen.
authors.forename: D
authors.initials: D
authors.lastname: Grandt
authors.forename: P
authors.initials: P
authors.lastname: Dahms
authors.forename: M
authors.initials: M
authors.lastname: Schimiczek
authors.forename: V E
authors.initials: VE
authors.lastname: Eysselein
authors.forename: J R
authors.initials: JR
authors.lastname: Reeve
authors.forename: R
authors.initials: R
authors.lastname: Mentlein
mesh_terms: D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000502:physiology*; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism*; D019894:Peptide YY; D010455:Peptides / Q000378:metabolism*; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D017476:Receptors, Neuropeptide Y; D019894:Peptide YY; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4
vernacular_title: Proteolytisches Processing durch Dipeptidyl-Aminopeptidase IV generiert Rezeptorselektivität für Peptid YY (PYY).