Development and evaluation of rapid monoclonal antibody-based coagglutination test for direct detection of Vibrio cholerae O139 synonym Bengal in stool samples.
A monoclonal antibody-based coagglutination test directly detected Vibrio cholerae O139 synonym Bengal in 83 of 120 watery diarrheal stool specimens; on culture, 90 samples were positive. Thus, with 92% sensitivity, 100% specificity, and 100% positive and 95% negative predictive values, the coagglutination test is a useful rapid test for V. cholerae O139.
Qadri
F
F
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
Chowdhury
A
A
Hossain
J
J
Chowdhury
K
K
Azim
T
T
Shimada
T
T
Islam
K M
KM
Sack
R B
RB
Albert
M J
MJ
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002771:Cholera / Q000175:diagnosis* / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D006801:Humans; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors; D014734:Vibrio cholerae / Q000276:immunology / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal