Inhibition of human peripheral blood lymphocyte function by protoporphyrin and longwave ultraviolet light.
Modulation of immunologic effector cells by exogenous photoactive substances has been advanced as an underlying mechanism for the efficacy of various photochemotherapeutic regimens. It is also possible that endogenous photosensitizers, such as protoporphyrin, could similarly modify the function of immune cell types. We examined the effects of protoporphyrin plus longwave UV light on the ability of human PBL to proliferate in response to mitogens. Noncytotoxic dosages of protoporphyrin plus UV light suppressed PHA-stimulated proliferation of both PBMC and enriched T cells. CD8+ cells were more sensitive to this inhibitory effect than CD4+ cells. The inhibitory effect was also observed when proliferation was induced by the combination of a phorbol ester and ionomycin. Inhibition of PBMC proliferation was associated with inhibition of IL-2 secretion but proliferation was not restored with exogenous IL-2. Instead, the effect of protoporphyrin plus UV light may be on IL-2R. Cells treated with protoporphyrin and UV light did not display the increase in CD25 and the beta-chain of the IL-2R induced by PHA in control cells. In contrast to the effects of protoporphyrin and UV light on IL-2 and IL-2R alpha-chain protein expression, the accumulation of mRNA for these proteins induced by PHA was unaffected. None of the effects of protoporphyrin plus UV light on lymphocytes were observed in control experiments where cells were treated with either protoporphyrin or UV light alone. We conclude that biologically relevant dosages of protoporphyrin and UV light modify the function of circulating lymphocytes.
Barrett
K E
KE
Division of Allergy and Immunology, University of California, School of Medicine, San Diego 92103.
Yen
A
A
Bigby
T D
TD
Montisano
D
D
Gigli
I
I
D001483:Base Sequence; D002470:Cell Survival / Q000187:drug effects; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects*; D008969:Molecular Sequence Data; D011701:PUVA Therapy; D011524:Protoporphyrins / Q000494:pharmacology / Q000528:radiation effects*; D012333:RNA, Messenger / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
D017931:DNA Primers; D007376:Interleukin-2; D011524:Protoporphyrins; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2