Cloning of the mouse cDNA encoding DNA topoisomerase I and chromosomal location of the gene.
The mouse cDNA encoding DNA topoisomerase I (TopoI) was cloned and the nucleotide sequence of 3512 bp was determined. The cDNA clone contained an open reading frame encoding a protein of 767 amino acids (aa), which is 2 aa longer than its human counterpart. Overall aa sequence homology between the mouse and human, and between the mouse and yeast (Saccharomyces cerevisiae) sequences was 96% and 42%, respectively. The mouse TopI gene was mapped at position 54.5 on chromosome 2 from linkage analyses of a three-point cross test with Geg, Ada, and a as marker genes.
Koiwai
O
O
Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Japan.
Yasui
Y
Y
Sakai
Y
Y
Watanabe
T
T
Ishii
K
K
Yanagihara
S
S
Andoh
T
T
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA; D004264:DNA Topoisomerases, Type I / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D017386:Sequence Homology, Amino Acid
D004247:DNA; D004264:DNA Topoisomerases, Type I