Control of oxidative phosphorylation in AS-30D hepatoma mitochondria.
1. The distribution of control of the rate of state 3 respiration of AS-30D hepatoma mitochondria was determined. 2. The ATP/ADP carrier (flux control coefficient, Ci = 0.70) and the ATP synthase (Ci = 0.19-0.32) were the only steps that exerted significant control on the phosphorylating flux supported by either glutamate+malate, pyruvate+malate, or succinate+rotenone. This is in contrast to liver mitochondria where the control is distributed between several steps. 3. It is suggested that this pattern of control of phosphorylation in hepatoma mitochondria is a consequence of a lower content of adenine nucleotides or a higher content of Mg2+.
López-Gómez
F J
FJ
Departamento de Bioquímica, Instituto Nacional de Cardiología, México D.F.
Torres-Márquez
M E
ME
Moreno-Sánchez
R
R
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008293:Malates / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism; D010085:Oxidative Phosphorylation; D006180:Proton-Translocating ATPases / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D017208:Rats, Wistar; D012402:Rotenone / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
D000227:Adenine Nucleotides; D005971:Glutamates; D008293:Malates; D011773:Pyruvates; D013386:Succinates; D012402:Rotenone; D018698:Glutamic Acid; C030298:malic acid; D019289:Pyruvic Acid; D000226:Mitochondrial ADP, ATP Translocases; D019802:Succinic Acid; D006180:Proton-Translocating ATPases; D008274:Magnesium