Thermal inactivation of oral polio vaccine: contribution of RNA and protein inactivation.
Heating the Sabin strains of poliovirus at 42 to 45 degrees C caused inactivation, loss of native antigen, and release of the viral RNA (vRNA). The loss of virion infectivity exceeded the loss of vRNA infectivity (as measured by transfection) by roughly 2 log10. Pirodavir inhibited the loss of native antigen and RNA release and reduced the loss of virion infectivity to the same level as the loss of vRNA infectivity. Thermoinactivation thus involves an RNA and a protein component, and pirodavir protected only against the latter.
Rombaut
B
B
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Verheyden
B
B
Andries
K
K
Boeyé
A
A
D000998:Antiviral Agents / Q000633:toxicity; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006358:Hot Temperature; D006801:Humans; D007668:Kidney; D008715:Methionine / Q000378:metabolism; D010880:Piperidines / Q000633:toxicity; D017955:Poliovirus / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D011055:Poliovirus Vaccine, Oral / Q000096:biosynthesis / Q000737:chemistry*; D011724:Pyridazines / Q000633:toxicity; D012367:RNA, Viral / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D014162:Transfection; D014529:Uridine / Q000378:metabolism; D015164:Vaccines, Inactivated; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry*
D000998:Antiviral Agents; D010880:Piperidines; D011055:Poliovirus Vaccine, Oral; D011724:Pyridazines; D012367:RNA, Viral; D015164:Vaccines, Inactivated; D014764:Viral Proteins; D008715:Methionine; C074589:pirodavir; D014529:Uridine