title: Uptake and metabolism of glutamate and aspartate by astroglial and neuronal preparations of rat cerebellum.
abstract: Astrocytes, neuronal perikarya and synaptosomes were prepared from rat cerebellum. Kinetics of high and low affinity uptake systems of glutamate and aspartate, nominal rates of 14CO2 production from [U-14C]glutamate, [U-14C]aspartate and [1-14C]glutamate and activities of enzymes of glutamate metabolism were studied in these preparations. The rate of uptake and the nomial rate of production of 14CO2 from these amino acids was higher in the astroglia than neuronal perikarya and synaptosomes. Activities of glutamine synthetase and glutamate dehydrogenase were higher in astrocytes than in neuronal perikarya and synaptosomes. Activities of glutaminase and glutamic acid decarboxylase were observed to be highest in neuronal perikarya and synaptosomes respectively. These results are in agreement with the postulates of theory of metabolic compartmentation of glutamate while others (presence of glutaminase in astrocytes and glutamine synthetase in synaptosomes) are not. Results of this study also indicated that (i) at high extracellular concentrations, glutamate/aspartate uptake may be predominantly into astrocytes while at low extracellular concentrations, it would be into neurons (ii) production of alpha-ketoglutarate from glutamate is chiefly by way of transamination but not by oxidative deamination in these three preparations and (iii) there are topographical differences glutamate metabolism within the neurons.
authors.affiliation: School of Life Sciences, University of Hyderabad, India.
authors.forename: V L
authors.initials: VL
authors.lastname: Rao
authors.forename: C R
authors.initials: CR
authors.lastname: Murthy
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D001253:Astrocytes / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002525:Cerebellar Cortex / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes
chemical_list: D005971:Glutamates; D002245:Carbon Dioxide; D001224:Aspartic Acid; D018698:Glutamic Acid; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase