title: Host cell membrane proteins on human immunodeficiency virus type 1 after in vitro infection of H9 cells and blood mononuclear cells. An immuno-electron microscopic study.
abstract: Human immunodeficiency virus type 1 (HIV-1)-infected H9 and blood mononuclear cells (MNCs) were studied by immunogold electron microscopy for the presence of HIV-1 gag p24 protein, env gp41 and gp120 proteins, and host cell molecules CD4, CD11a, CD25, CD54, CD63, HLA class I and HLA-DR. Uninfected H9 cells and MNC membranes labelled for CD4, HLA class I and class II, and, at low density, CD11a and CD54; lysosomal structures in the cytoplasm labelled for CD63. The infected cell surface showed immunolabelling for HIV-1 proteins, as did budding particle-like structures. Immunogold labelling of the cell membrane for CD4 was almost non-existent. The level of immunolabelling for CD11a and CD54 on infected cells was greater than that on uninfected cells; this is presumably related to a state of activation during virus synthesis. Budding particle-like structures and free virions in the intercellular space were immunogold-labelled for all host cell markers investigated. This was confirmed by double immunogold labelling using combinations of HIV-1 gag p24 labelling and labelling for the respective host cell molecule. We conclude that virions generated in HIV-1-infected cells concentrate host-derived molecules on their envelope. Also molecules with a prime function in cellular adhesion concentrate on the virion.
authors.affiliation: Department of Pathology, University Hospital, Utrecht, The Netherlands.
authors.forename: T
authors.initials: T
authors.lastname: Meerloo
authors.forename: M A
authors.initials: MA
authors.lastname: Sheikh
authors.forename: A C
authors.initials: AC
authors.lastname: Bloem
authors.forename: A
authors.initials: A
authors.lastname: de Ronde
authors.forename: M
authors.initials: M
authors.lastname: Schutten
authors.forename: C A
authors.initials: CA
authors.lastname: van Els
authors.forename: P J
authors.initials: PJ
authors.lastname: Roholl
authors.forename: P
authors.initials: P
authors.lastname: Joling
authors.forename: J
authors.initials: J
authors.lastname: Goudsmit
authors.forename: H J
authors.initials: HJ
authors.lastname: Schuurman
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D018845:CD11 Antigens; D015815:Cell Adhesion Molecules / Q000032:analysis; D002460:Cell Line; D016655:HIV Core Protein p24 / Q000032:analysis; D015699:HIV Envelope Protein gp120 / Q000032:analysis; D015700:HIV Envelope Protein gp41 / Q000032:analysis; D015497:HIV-1 / Q000737:chemistry*; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D008565:Membrane Proteins / Q000032:analysis*; D016253:Microscopy, Immunoelectron
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D018845:CD11 Antigens; D015815:Cell Adhesion Molecules; D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D006680:HLA Antigens; D008565:Membrane Proteins; D018799:Intercellular Adhesion Molecule-1