[Comparison of the repetitive DNA sequences between Paragonimus westermani and Pagumogonimus skrjabini from six areas].
The genomic DNA of the two lung flukes (P. westermani and P. skrjabini) from six areas was digested separately by three restriction endonucleases BamHI, Hae III and Hind III, followed by agarose gel electrophoresis. Repetitive DNA were visualized as distinct bands in stained gels. Restriction fragment length differences were detected between the two species. The majority of bands of repetitive DNA were similar with minor differences within the same species collected from different areas, upon digestion with certain restriction endonucleases. The results demonstrated that the technique for demonstrating the band pattern of repetitive DNA with three restriction endonucleases can be used for the identification of P. westermani and P. skrjabini and the geographic relationship of the same species from different areas (Figs. 1-3).
Xiao
J H
JH
Department of Parasitology, Hengyang Medical College.
Chen
C E
CE
Zhang
W C
WC
Nie
C X
CX
Li
B W
BW
D000818:Animals; D002681:China; D004247:DNA / Q000235:genetics; D010238:Paragonimus / Q000145:classification / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D004247:DNA