Potentiation of transforming growth factor (TGF-beta 1) by natural coral and fibrin in a rabbit cranioplasty model.
The association of a biodegradable material and a growth factor could be of clinical value for treating bone defects. We therefore tested the association of transforming growth factor beta (TGF-beta 1) in fibrin glue and coral granules to heal skull defects in rabbits. Adult rabbits underwent a double trepanation symmetrically in both parietal bones. Using histomorphometry, we compared bone repair after 1 month in control animals (n = 5) and in animals treated with either TGF-beta 1 as a single injection of 1 microgram in methylcellulose (n = 5) or in fibrin glue (n = 5), or with coral granules in fibrin glue (n = 4) or with coral granules and TGF-beta 1 1 microgram in fibrin glue (n = 5). We measured the diameter of the remaining defect and the surface of the bone growth. TGF-beta 1 without coral in either methyl cellulose or fibrin induced a partial closure of the defect as assessed by a significant decrease in the defect diameter, compared with the control group. However, the association of TGF-beta 1 in fibrin and coral induced an area of the bone growth higher than in any other groups (P < 0.05). Two months after surgery, this triple association induced a better healing of the defect than coral alone or control group. In each group treated with TGF-beta 1, the mineralization rate was increased not only at the treated side but also in the contralateral defect which was untreated, suggesting a diffusion of the growth factor.(ABSTRACT TRUNCATED AT 250 WORDS)
Arnaud
E
E
INSERM U349, Centre Viggo Petersen, Paris.
Morieux
C
C
Wybier
M
M
de Vernejoul
M C
MC
D000818:Animals; D001846:Bone Development / Q000187:drug effects; D001842:Bone and Bones / Q000166:cytology / Q000000981:diagnostic imaging / Q000378:metabolism; D002119:Calcium Carbonate / Q000378:metabolism / Q000494:pharmacology*; D004357:Drug Synergism; D005337:Fibrin / Q000378:metabolism / Q000494:pharmacology*; D008954:Models, Biological; D011817:Rabbits; D011859:Radiography; D012886:Skull / Q000166:cytology / Q000000981:diagnostic imaging / Q000601:surgery*; D013518:Surgery, Plastic; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000494:pharmacology*; D014207:Trephining
D016212:Transforming Growth Factor beta; D005337:Fibrin; D002119:Calcium Carbonate