title: A rat brain cDNA encoding the neurotransmitter transporter with an unusual structure.
abstract: A rat cDNA clone encoding the novel membrane protein of the neurotransmitter transporters family was cloned and sequenced. The cDNA was identified as a transcript of the gene NTT4 of which a partial genomic clone was previously sequenced. Alignment of the amino acid sequence of NTT4 with other members of the neurotransmitter transporter family revealed a marked deviation from the conserved structure of all other members of the family. The largest extracellular loop with a potential glycosylation site was identified between membrane segments 7 and 8. The protein retains the common glycosylated loop between transmembrane helices 3 and 4 in all members of the family. The transcript of NTT4 was found exclusively in the central nervous system and is more abundant in the cerebellum and the cerebral cortex.
authors.affiliation: Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
authors.forename: Q R
authors.initials: QR
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Mandiyan
authors.forename: B
authors.initials: B
authors.lastname: López-Corcuera
authors.forename: H
authors.initials: H
authors.lastname: Nelson
authors.forename: N
authors.initials: N
authors.lastname: Nelson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D008562:Membrane Glycoproteins / Q000235:genetics*; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D050480:Plasma Membrane Neurotransmitter Transport Proteins; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment
chemical_list: D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D050480:Plasma Membrane Neurotransmitter Transport Proteins; D012333:RNA, Messenger; C079036:Slc6a17 protein, rat; D004247:DNA