Purification and characterization of two new c-type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans.
Two new c-type cytochromes have been purified from cell membranes of the acidophilic Thiobacillus ferrooxidans. In contrast to a soluble cytochrome c with molecular mass of 14 kDa reported earlier, a membrane-bound cytochrome c with a mass of 21 kDa was solubilized with octylthioglucoside and purified to homogeneity. In addition, a high molecular mass c-type cytochrome (68 kDa) was also solubilized and purified using Triton X-100 as a detergent. Both acid-stable species are partially released during osmotic shock and chloroform treatment of the bacteria; they are integral components in the respiratory chain donating electrons to the terminal cytochrome oxidase.
Valkova-Valchanova
M B
MB
Department of Biology, Syracuse University, NY 13244.
Chan
S H
SH
D000596:Amino Acids / Q000032:analysis; D001400:Azurin / Q000031:analogs & derivatives / Q000494:pharmacology; D002413:Cations, Divalent / Q000378:metabolism; D002451:Cell Compartmentation; D003574:Cytochrome c Group / Q000302:isolation & purification* / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D013053:Spectrophotometry; D013855:Thiobacillus / Q000201:enzymology*
D000596:Amino Acids; D002413:Cations, Divalent; D003574:Cytochrome c Group; C012543:rusticyanin; D001400:Azurin; D007501:Iron