title: Relationship between tissue factor expression and deposition of fibrin, platelets, and leukocytes on cultured endothelial cells under venous blood flow conditions.
abstract: Endothelial cell-mediated coagulation and leukocyte adhesion are processes that might be connected by the generation of thrombin. To examine the interaction of procoagulant and proadhesive activity, cultures of endothelial cells were stimulated with tumor necrosis factor-alpha, which resulted in the surface expression of tissue factor. Subsequent exposure to human nonanticoagulated blood at a shear rate of 100 s-1 in a parallel plate perfusion device led to the deposition of polymerized fibrin, which covered 63% of the endothelial surface. In addition, numerous platelet aggregates (71 per 10 mm cross-section) and leukocytes (53 +/- 6/mm2) were deposited on stimulated endothelial cells, whereas no fibrin and only a few platelet aggregates (4 +/- 1 per 10 mm cross-section) and leukocytes (6 +/- 1/mm2) were detected on control cells. A significant portion of the adherent leukocytes bound to fibrin and platelets. However, when the deposition of fibrin and platelet aggregates was inhibited with the anti-tissue factor antibody HTFI-7B8 by 100% and 86%, respectively, leukocyte adherence remained unchanged (68 +/- 6/mm2). This indicated that leukocytes could efficiently adhere to endothelial cells through direct cell-cell contact independent of both thrombin and deposited fibrin. Moreover, this direct adhesion of leukocytes to the endothelial surface was reduced twofold to threefold when fibrin deposition occurred. These data suggest a relationship between endothelial procoagulant and proadhesive properties in that tissue factor-initiated coagulation may contribute to leukocyte adhesion through the formation of an adhesive fibrin/platelet meshwork but concurrently prevents the adhesive endothelial surface to bind leukocytes at its full capacity.
authors.affiliation: F. Hoffmann-La Roche Ltd, Basel, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Kirchhofer
authors.forename: K S
authors.initials: KS
authors.lastname: Sakariassen
authors.forename: M
authors.initials: M
authors.lastname: Clozel
authors.forename: T B
authors.initials: TB
authors.lastname: Tschopp
authors.forename: P
authors.initials: P
authors.lastname: Hadváry
authors.forename: Y
authors.initials: Y
authors.lastname: Nemerson
authors.forename: H R
authors.initials: HR
authors.lastname: Baumgartner
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D001769:Blood; D001792:Blood Platelets / Q000502:physiology*; D002448:Cell Adhesion; D002450:Cell Communication; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005337:Fibrin / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000276:immunology / Q000502:physiology*; D016256:Lewis X Antigen; D009000:Monocytes / Q000502:physiology; D009504:Neutrophils / Q000502:physiology; D010477:Perfusion; D010973:Platelet Adhesiveness; D013925:Thromboplastin / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D015703:Antigens, CD; D016256:Lewis X Antigen; D014409:Tumor Necrosis Factor-alpha; D005337:Fibrin; D013925:Thromboplastin