title: Effects of intraoperative radiotherapy on vascular grafts in a canine model.
abstract: OBJECTIVE
  The effects of intraoperative radiotherapy +/- external beam radiotherapy on prosthetic vascular grafts were investigated in a canine model.
  
  
  METHODS
  In 1986 and 1987, 30 adult beagles underwent laparotomy with transection and segmental resection of the infrarenal aorta followed by immediate reconstruction with a prosthetic graft. Intraoperative radiotherapy at varying doses from 0-30 Gy was then administered to all animals. Half of the dogs received 36 Gy external beam radiotherapy in 10 fractions postoperatively. Animals were sacrificed and necropsied at predetermined intervals and as clinically indicated to assess early (< or = 6 months) and late (> 6 months) effects to the vascular graft and surrounding normal tissue.
  
  
  RESULTS
  Histopathologic analyses of irradiated vascular structures were performed and correlations were made with the clinical outcome. The most frequent early clinical toxicity was graft thrombosis, occurring in 7 of 10 animals followed for < or = 6 months. Early graft thrombus formation appeared unrelated to radiotherapy dose and probably represented a technical surgical complication. Anastomotic stenosis of varying severity occurred in most animals followed > 6 months. Late (> 6 months) graft stenosis was correlated with intraoperative radiotherapy dose. At < or = 20 Gy of intraoperative irradiation, 3 of 14 animals developed late graft occlusion; at > 25 Gy, five of six animals developed late occlusion. On histopathologic review, increasing intraoperative dose and increasing total radiotherapy dose (intraoperative+external beam) appeared to correspond with increasing severity of graft changes seen after 6 months of follow-up.
  
  
  CONCLUSIONS
  Thrombus formation is a frequent early complication of vascular graft placement of the infrarenal aorta in our beagle dog model. Intraoperative doses up to 20 Gy appear to contribute minimally to late graft occlusion, while doses > or = 25 Gy contribute to late occlusion with high likelihood. Both intraoperative dose and total radiotherapy dose correlated with late graft occlusion, and with histopathologic changes in the graft and anastomoses.
authors.affiliation: Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
authors.forename: P A
authors.initials: PA
authors.lastname: Johnstone
authors.forename: M
authors.initials: M
authors.lastname: Sprague
authors.forename: A M
authors.initials: AM
authors.lastname: DeLuca
authors.forename: J D
authors.initials: JD
authors.lastname: Bacher
authors.forename: V A
authors.initials: VA
authors.lastname: Hampshire
authors.forename: R E
authors.initials: RE
authors.lastname: Terrill
authors.forename: T J
authors.initials: TJ
authors.lastname: Kinsella
authors.forename: W F
authors.initials: WF
authors.lastname: Sindelar
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology / Q000528:radiation effects* / Q000601:surgery*; D001807:Blood Vessel Prosthesis; D004285:Dogs; D005260:Female; D006083:Graft Occlusion, Vascular / Q000209:etiology; D007430:Intraoperative Care; D008954:Models, Biological; D011833:Radiation Injuries, Experimental / Q000209:etiology; D011836:Radiation Tolerance; D013927:Thrombosis / Q000209:etiology