title: Widespread expression of inositol 1,4,5-trisphosphate receptor type 1 gene (Insp3r1) in the mouse central nervous system.
abstract: The expression of inositol 1,4,5-trisphosphate receptor type 1 (InsP3R1) in the mouse central nervous system (CNS) was studied by in situ hybridization. The receptor mRNAs were widely localized throughout the CNS, predominantly in the olfactory tubercle, cerebral cortex, CA1 pyramidal cell layer of the hippocampus, caudate putamen, and cerebellar Purkinje cells, where phosphoinositide turnover is known to be stimulated by various neurotransmitter receptors. In the most abundantly expressing Purkinje cells, InsP3R1 mRNA appeared to be translocated to the distal dendrites, since a strong hybridization density was observed in the molecular layer of the cerebellum. InsP3R protein is known to form tetrameric receptor-channel complex. Our preliminary hybridization data using probes for three distinct InsP3R subtypes showed preferential expression of InsP3R1 in many parts of the CNS. The expression of other receptor subtypes (InsP3R2 and InsP3R3) is less efficient, suggesting that a homotetramer formed of InsP3R1 subtype may play a central part in InsP3/Ca2+ signalling in the neuronal function, whereas a homotetramer of other subtypes and a possible heterotetramer among subtypes may be involved in differential InsP3/Ca2+ signalling. The chromosomal localization of the gene coding for InsP3R1 was confirmed on chromosome 6 but was found to be genetically independent of the Lurcher (Lc) mutation.
authors.affiliation: Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Furuichi
authors.forename: D
authors.initials: D
authors.lastname: Simon-Chazottes
authors.forename: I
authors.initials: I
authors.lastname: Fujino
authors.forename: N
authors.initials: N
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.forename: A
authors.initials: A
authors.lastname: Miyawaki
authors.forename: S
authors.initials: S
authors.lastname: Yoshikawa
authors.forename: J L
authors.initials: JL
authors.lastname: Guénet
authors.forename: K
authors.initials: K
authors.lastname: Mikoshiba
mesh_terms: D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D015220:Calcium Channels / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism*; D002874:Chromosome Mapping; D015870:Gene Expression; D017403:In Situ Hybridization; D053496:Inositol 1,4,5-Trisphosphate Receptors; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008818:Mice, Neurologic Mutants; D009115:Muridae; D010716:Phosphatidylinositols / Q000378:metabolism; D011689:Purkinje Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D010716:Phosphatidylinositols; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear