Assignment of xeroderma pigmentosum group C (XPC) gene to chromosome 3p25.
The human gene XPC (formerly designated XPCC), which corrects the repair deficiency of xeroderma pigmentosum (XP) group C cells, was mapped to 3p25. A cDNA probe for Southern blot hybridization and diagnostic PCR analyses of hybrid clone panels informative for human chromosomes in general and portions of chromosome 3 in particular produced the initial results. Fluorescence in situ hybridization utilizing both a yeast artificial chromosome DNA containing the gene and XPC cDNA as probes provided verification and specific regional assignment. A conflicting assignment of XPC to chromosome 5 is discussed in light of inadequacies in the exclusive use of microcell-mediated chromosome transfer for gene mapping.
Legerski
R J
RJ
Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Liu
P
P
Li
L
L
Peterson
C A
CA
Zhao
Y
Y
Leach
R J
RJ
Naylor
S L
SL
Siciliano
M J
MJ
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D006224:Cricetinae; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014983:Xeroderma Pigmentosum / Q000145:classification / Q000235:genetics*