title: [Cloning using nuclear transfer in bovine species: initial results].
abstract: In 1987, Prather et al. have performed the first embryo cloning by nuclear transfer in the bovine species. Since, many researchers try to develop and to apply the technique. While the enucleation of the recipient oocyte, the injection of the donor blastomere and the fusion procedure are now well controlled, on the other hand, maturation and activation as the development and freezing of the cloned embryos need to be more investigated. The cloned embryo is more fragile. An increase in embryonic mortality is observed after transfer in a recipient cow.
authors.affiliation: Centre de Recherches IRSIA-CERAD 2, Université de Liège.
authors.forename: F
authors.initials: F
authors.lastname: Ectors
authors.forename: F J
authors.initials: FJ
authors.lastname: Ectors
authors.forename: A
authors.initials: A
authors.lastname: Delval
authors.forename: F
authors.initials: F
authors.lastname: Thonon
authors.forename: J F
authors.initials: JF
authors.lastname: Beckers
mesh_terms: D000818:Animals; D001757:Blastomeres / Q000502:physiology / Q000637:transplantation; D002417:Cattle / Q000502:physiology*; D003001:Cloning, Molecular / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D008846:Micromanipulation / Q000379:methods; D009865:Oocytes / Q000502:physiology; D013480:Superovulation; D015044:Zona Pellucida / Q000502:physiology
vernacular_title: Le clonage par transfert de noyau dans l'espèce bovine: premiers résultats.