[The influence of cations on the letality and on the formation of the toxin of Pasteurella multocida].
The stability of the Pasteurella multocida toxin has proven as very pH dependent. Therefore, a pH lower than 6 should be avoided. A concentration of 1 mM Cu2+ decreased the lethal effect of the toxin but the equimolecular addition of chelators compensated this effect. Possible structure-effect mechanisms were discussed. Iron supplement (100 microM) to two culture media examined did not influence the production of the toxin but during the cultivation in the low-iron medium TDHM the toxin level increased. This increased toxin production was not due to a more intensive multiplication of bacteria.
Erler
W
W
Institut für Veterinärmedizin (Robert von Ostertag-Institut) des Bundesgesundheitsamtes, Jena, Deutschland.
Jacob
B
B
Schlegel
J
J
D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000633:toxicity; D001681:Biological Assay; D015169:Colony Count, Microbial; D003300:Copper / Q000494:pharmacology*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology*; D051379:Mice; D016979:Pasteurella multocida / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; C062258:Pasteurella multocida toxin; D003300:Copper; D007501:Iron
The influence of cations on the lethality and on the formation of the toxin of Pasteurella multocida.