title: Antibodies specific for human thyrotropin receptor induce MHC antigen expression in thyroid cells.
abstract: Autoantibodies (AAbs) to hormone receptors are found in autoimmune diseases such as Graves' disease (GD) or myasthenia gravis. A structural link between hormone receptor and MHC genes has been documented, suggesting a possible co-regulation of MHC and hormone receptor genes. Thus, in vitro experiments were designed to search for a pathologic role for AAbs. In a model study, we investigated whether adding murine anti-human thyrotropin receptor mAbs would affect MHC gene expression in either cloned human thyroid epithelial cell or primary murine thyroid epithelial cell cultures. We found that two anti-human thyrotropin receptor monoclonal AAbs, 11E7 and 34A, induced, with an intensity comparable to that of IFN-gamma, transcription and expression of class I and class II/Ii chain proteins in human and murine thyroid epithelial cells. Two other anti-human thyrotropin receptor mAbs, 12E3 and 243-3, were ineffective. These data suggest a new role for autoantibodies in the pathology of autoimmune endocrinopathies.
authors.affiliation: INSERM U 283, Cochin Hospital, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Ropars
authors.forename: S
authors.initials: S
authors.lastname: Marion
authors.forename: L
authors.initials: L
authors.lastname: Takorabet
authors.forename: J
authors.initials: J
authors.lastname: Braun
authors.forename: J
authors.initials: J
authors.lastname: Charreire
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006681:HLA-D Antigens / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011989:Receptors, Thyrotropin / Q000378:metabolism*; D013961:Thyroid Gland / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D017931:DNA Primers; D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D011989:Receptors, Thyrotropin; C045084:invariant chain