A 92-kDa human immunostimulatory protein.
We purified to apparent homogeneity a human urinary glycoprotein of 92 kDa (HGP.92) that, administered intravenously at 250 micrograms/kg, fully protected mice against a lethal inoculum of Listeria monocytogenes. Since HGP.92 protected scid mice, which lack B and T lymphocytes, this increased resistance to Listeria did not appear to be lymphocyte mediated. Furthermore, inflammatory macrophages incubated with 6 nM HGP.92 inhibited the growth of Lewis carcinoma cells in vitro. These two activities appeared to depend on an oligosaccharide moiety, as they were lost after N-Glycanase treatment of HGP.92. Thus, the biological activity of HGP.92 was in some way related to a glycan moiety.
Fontan
E
E
Unité d'Immunophysiologie Cellulaire, Institut Pasteur, Paris, France.
Briend
E
E
Saklani-Jusforgues
H
H
d'Alayer
J
J
Vandekerckhove
J
J
Fauve
R M
RM
D000595:Amino Acid Sequence; D000818:Animals; D002277:Carcinoma / Q000517:prevention & control; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D007111:Immunity, Cellular; D008089:Listeria monocytogenes / Q000276:immunology; D008088:Listeriosis / Q000517:prevention & control*; D008175:Lung Neoplasms / Q000517:prevention & control; D008213:Lymphocyte Activation / Q000187:drug effects; D008262:Macrophage Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009088:Mucoproteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000737:chemistry; D013154:Spleen / Q000166:cytology; D013329:Structure-Activity Relationship; D058949:Uromodulin
D006023:Glycoproteins; D009088:Mucoproteins; D010446:Peptide Fragments; C548612:UMOD protein, human; C548613:Umod protein, mouse; D058949:Uromodulin