title: Release of GABA from rat hippocampal slices: involvement of quisqualate/N-methyl-D-aspartate-gated ionophores and extracellular magnesium.
abstract: Effects of glutamate agonists and their selective antagonists on the Ca(2+)-dependent release of [3H]GABA from rat hippocampal slices were studied in a superfusion system. The release was enhanced by glutamate, kainate, quisqualate, N-methyl-D-aspartate and glycine in a concentration-dependent manner. In general, (+)-5-methyl-10,11-dihydro-5H-dibenzo(a,d)cyclohept-5,10-imine hydrogen maleate (MK-801) strongly antagonized the effects of all agonists tested in low-Mg2+ (0.1 mM) media. 6-Cyano-7-nitroquinoxaline-2,3-dione did not inhibit the effects of N-methyl-D-aspartate, quisqualate, glycine or N-methyl-D-aspartate together with glycine in 0.1 mM Mg2+ media. Extracellular Mg2+ failed to block the release elicited by the agonists alone. However, the action of N-methyl-D-aspartate was potentiated by glycine only in low-Mg2+ media. We suggest that under the present experimental conditions the activation of N-methyl-D-aspartate/low-affinity quisqualate receptors located presynaptically on excitatory fibre terminals and/or postsynaptically on GABAergic interneurons is essential in hippocampal GABA release. A functional interaction is proposed between glycine and extracellular Mg2+ in the modulation of the quisqualate N-methyl-D-aspartate-gated ionophores involved in this process.
authors.affiliation: Tampere Brain Research Center, Department of Biomedical Sciences, University of Tampere, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Janáky
authors.forename: P
authors.initials: P
authors.lastname: Saransaari
authors.forename: S S
authors.initials: SS
authors.lastname: Oja
mesh_terms: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D000818:Animals; D016291:Dizocilpine Maleate / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D005998:Glycine / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects*; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA; D017470:Receptors, Glutamate / Q000187:drug effects / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005971:Glutamates; D011810:Quinoxalines; D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D016291:Dizocilpine Maleate; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D008274:Magnesium; D005998:Glycine