[Review: recent studies on a human eosinophilic leukemia cell line, EoL as an experimental model of eosinophils].
Recently, a human eosinophilic leukemia cell line, EoL was established from an eosinophilic leukemia patient. EoL-1 cells have the cytohistologic features of myeloblasts under normal culture conditions, and they can be induced to differentiate into eosinophilic granule-containing cells but not into other lineage cells under several culture conditions and are therefore considered to be committed precursors of eosinophils. Furthermore, EoL-1 cells can also be induced to differentiate functionally to show PAF-induced Ca2+ influx and actin polymerization. On the other hand, EoL-3 cells show constitutive expression of Fc epsilon RII, Fc gamma RII, LFA-1 and ICAM-1 on their cell surface. The EoL cells may provide new information on some aspects of the signal transduction mechanisms involved in the proliferation, differentiation and activation of eosinophils.
Morita
M
M
Department of Pediatrics, Faculty of Medicine, Kyoto University.
D000199:Actins / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D004804:Eosinophils / Q000166:cytology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D015472:Leukemia, Eosinophilic, Acute / Q000378:metabolism / Q000473:pathology*; D011108:Polymers; D011961:Receptors, Fc / Q000378:metabolism; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D000199:Actins; D015815:Cell Adhesion Molecules; D011108:Polymers; D011961:Receptors, Fc; D018799:Intercellular Adhesion Molecule-1; D002118:Calcium