title: Relationship between corticotropin-releasing factor and interleukin-2: evolutionary evidence.
abstract: The addition of corticotropin-releasing factor (CRF) to molluscan hemocytes induces the release of biogenic amines (norepinephrine, epinephrine, dopamine), a phenomenon we have considered as an ancestral type of stress response [(1992) Gen. Comp. Endocrinol. 87, 354-360]. A similar but less significant response was observed following the addition of interleukin-2 (IL-2). Pre-incubation of hemocytes with IL-2 or anti-IL-2 monoclonal antibody significantly reduced or completely eliminated the CRF-induced release of biogenic amines. Further direct evidence of competition between CRF and IL-2 was revealed by immunocytochemical and cytofluorimetric analysis. The data are compatible with the presence of a unique (ancestral?) receptor on molluscan hemocytes, capable of binding both CRF and IL-2, two key molecules of the neuroendocrine and immune system, respectively.
authors.forename: E
authors.initials: E
authors.lastname: Ottaviani
authors.forename: A
authors.initials: A
authors.lastname: Franchini
authors.forename: E
authors.initials: E
authors.lastname: Caselgrandi
authors.forename: A
authors.initials: A
authors.lastname: Cossarizza
authors.forename: C
authors.initials: C
authors.lastname: Franceschi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D005075:Biological Evolution; D003346:Corticotropin-Releasing Hormone / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D007124:Immunoenzyme Techniques; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D012908:Snails
chemical_list: D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011956:Receptors, Cell Surface; D003346:Corticotropin-Releasing Hormone