title: Comparison of agar dilution, broth dilution, and disk diffusion testing of ampicillin against Haemophilus species by using in-house and commercially prepared media.
abstract: An evaluation to determine the optimal method for the in vitro susceptibility testing of Haemophilus strains to ampicillin was undertaken. In our hands, in-house-prepared Haemophilus Test Medium used by either the broth macrodilution or agar dilution method produced the most consistent results, especially for beta-lactamase-negative, ampicillin-resistant H. influenzae strains.
authors.affiliation: Department of Laboratory Medicine, Mayo Clinic and Foundation Rochester, Minnesota 55905.
authors.forename: P C
authors.initials: PC
authors.lastname: Kohner
authors.forename: J E
authors.initials: JE
authors.lastname: Rosenblatt
authors.forename: F R
authors.initials: FR
authors.lastname: Cockerill
mesh_terms: D000362:Agar; D000668:Ampicillin Resistance; D003470:Culture Media; D006190:Haemophilus / Q000145:classification / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006193:Haemophilus influenzae / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D003470:Culture Media; D000362:Agar