Beta-adrenoceptor density and subtype distribution in cerebellum and hippocampus from patients with Alzheimer's disease.
beta-Adrenoceptor density and beta 1- and beta 2-subtype distribution were examined in hippocampi and cerebella from patients with Alzheimer's disease (AD/SDAT). Tissues from age-, sex and post-mortem delay matched non-demented patients served as controls. The total beta-adrenoceptor density as evaluated in saturation experiments with the hydrophilic radioligand [3H]CGP 12177 was higher in hippocampal (36-39 fmol/mg protein) than cerebellar tissues (20-21 fmol/mg), however, no differences were found in either brain region between AD/SDAT patients and controls. Subtype distribution using the highly selective beta 1-adrenoceptor antagonist CGP 20712A revealed a slightly higher proportion of beta 1-adrenoceptors in hippocampus (26%-27%) than in cerebellum (20%-21%) with, again, no difference between AD/SDAT and controls. We recently described a 40% reduction in basal and stimulated adenylyl cyclase activity in post-mortem hippocampus of AD/SDAT patients, a brain region greatly affected by the disease, the present data now demonstrate that this disease-related effect is not mirrored by a similar change in beta-adrenoceptor density nor in subtype distribution.
Lemmer
B
B
Zentrum der Pharmakologie, J.W. Goethe-Universität Frankfurt, Germany.
Langer
L
L
Ohm
T
T
Bohl
J
J
D000319:Adrenergic beta-Antagonists / Q000378:metabolism / Q000494:pharmacology; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism*; D001667:Binding, Competitive; D002531:Cerebellum / Q000378:metabolism / Q000648:ultrastructure*; D003704:Dementia / Q000378:metabolism; D005260:Female; D006624:Hippocampus / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D007093:Imidazoles / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D011412:Propanolamines / Q000378:metabolism / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D014316:Tritium
D000319:Adrenergic beta-Antagonists; D007093:Imidazoles; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; D014316:Tritium; C049763:CGP 20712A; C033394:CGP 12177