Differential regulation of LFA-1 and ICAM-1 on human primary B-lymphocytes.
Changes in adhesive properties play important regulatory roles in activation and differentiation of B-cells. To better understand the regulation of interactions between B-cells and other cells during the immune response, we have studied surface expression of the adhesion molecules LFA-1 (CD11a/CD18) and ICAM-1 (CD54). Both adhesion molecules were upregulated during B-cell activation. However, upon stimulation with anti-IgM and IL-4, ICAM-1 levels started to increase within 12 hr, while LFA-1 levels did not start to increase until after 36 hr. When B-cells were stimulated with the PKC activator PDB and a calcium ionophore, ICAM-1 levels, but not LFA-1 levels, increased. Only if these activators were removed after around 24 hr of activation and the cells were recultured in fresh medium was there an eightfold induction of LFA-1. Such reculturing in fresh medium led, however, to decreased ICAM-1 levels.
Brändén
H
H
Department for Cell and Molecular Biology, University of Umeå, Sweden.
Lundgren
E
E
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D007075:Immunoglobulin M; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D015759:Ionomycin