Divinyl sulfone cross-linked hyperpolymeric human haemoglobin as an artificial oxygen carrier in anaesthetized spontaneously breathing rats.
The production of hyperpolymer haemoglobins, exhibiting sufficiently low colloid osmotic pressure and sufficiently low viscosity is possible, even in concentrations, and therewith oxygen transport capacity, high enough to supply an organism adequately with oxygen. Such hyperpolymers, when infused, are tolerated by anaesthetized rats in acute blood exchange experiments. Ex vivo determinations of plasma colloid osmotic pressure and both, plasma and whole blood kinematic viscosity during blood exchange showed, that corresponding properties found in vivo were refound within the animal. Furthermore we could show that hyperpolymers produced from desoxygenated human haemoglobin with divinyl sulfone as a crosslinker take part in tissue supply of oxygen to a substantial degree (about 50%) without and with increased inspiratory oxygen fraction, demonstrating the principal ability of hyperpolymers to transport oxygen in blood and to deliver it to tissues.
Pötzschke
H
H
Institut für Physiologie und Pathophysiologie, Johannes Gutenberg-Universität, Mainz, BR Deutschland.
Guth
S
S
Barnikol
W K
WK
D000818:Animals; D001693:Biological Transport, Active; D001802:Blood Substitutes / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001809:Blood Viscosity; D003432:Cross-Linking Reagents; D006454:Hemoglobins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009997:Osmotic Pressure; D010100:Oxygen / Q000097:blood / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013450:Sulfones
D001802:Blood Substitutes; D003432:Cross-Linking Reagents; D006454:Hemoglobins; D013450:Sulfones; C037158:polyhemoglobin; C009873:divinyl sulfone; D010100:Oxygen