In vitro neutralization of T-2 toxin toxicity by a monoclonal antibody.
A T-2 toxin specific monoclonal antibody, IgG1 K, with a low level of ELISA cross-reactivity to Acetyl T-2, HT-2, and iso T-2 toxins has been produced. The ability of this monoclonal antibody to neutralize the cytotoxicity of T-2 toxin in PHA stimulated cultures of human lymphocytes was determined by the MTT method. The complete neutralization of the toxic effect of 0.02 microM T-2 toxin was obtained with 0.03 microM of MoAb, whereas the 50% neutralizing dose (ND50) was observed at 0.009 microM of MoAb. Partial neutralization was observed with Acetyl T-2 toxin (ND50 = 0.038 microM) and HT-2 (ND50 = 0.94 microM). These results could represent a rational for clinical use of T-2 toxin specific monoclonal antibody in prophylaxis and therapy of T-2 toxemia.
Minervini
F
F
Istituto Tossine e Micotossine da Parassiti Vegetali, Consiglio Nazionale delle Richerche, Bari, Italy.
Gyongyosi-Horvath
A
A
Lucivero
G
G
Visconti
A
A
Barna-Vetro
I
I
Solti
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000918:Antibody Specificity; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D009500:Neutralization Tests; D013605:T-2 Toxin / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000633:toxicity; D014255:Trichothecenes / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D014255:Trichothecenes; D013605:T-2 Toxin