Whitefly transmission and efficient ssDNA accumulation of bean golden mosaic geminivirus require functional coat protein.
Bean golden mosaic geminivirus (BGMV) has a bipartite genome composed of two circular ssDNA components (DNA-A and DNA-B) and is transmitted by the whitefly, Bemisia tabaci. DNA-A encodes the viral replication proteins and the coat protein. To determine the role of BGMV coat protein systemic infection and whitefly transmission, two deletions and a restriction fragment inversion were introduced into the BGMV coat protein gene. All three coat protein mutants produced systemic infections when coinoculated with DNA-B onto Phaseolus vulgaris using electric discharge particle acceleration "particle gun." However, they were not sap transmissible and coat protein was not detected in mutant-infected plants. In addition, none of the mutants were transmitted by whiteflies. With all three mutants, ssDNA accumulation of DNA-A and DNA-B was reduced 25- to 50-fold and 3- to 10-fold, respectively, as compared to that of wild-type DNA. No effect on dsDNA-A accumulation was detected and there was 2- to 5-fold increase in dsDNA-B accumulation. Recombinants between the mutated DNA-A and DNA-B forms were identified when the inoculated coat protein mutant was linearized in the common region.
Azzam
O
O
Department of Plant Pathology, University of Wisconsin at Madison 53706.
Frazer
J
J
de la Rosa
D
D
Beaver
J S
JS
Ahlquist
P
P
Maxwell
D P
DP
D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004175:Diptera / Q000382:microbiology*; D007887:Fabaceae / Q000382:microbiology; D017791:Geminiviridae / Q000235:genetics* / Q000378:metabolism; D017344:Genes, Insect / Q000235:genetics; D007303:Insect Vectors / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology; D010946:Plants, Medicinal; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D004277:DNA, Single-Stranded; D004279:DNA, Viral