title: Supercritical fluid extraction of polychlorinated biphenyls from lyophilized fish tissue.
abstract: A method for the rapid interference free analysis of polychlorinated biphenyl congeners (chlorinated biphenyls, CBs) in lyophilized fish tissue is presented. The method was developed on a lyophilized tuna muscle tissue that contained 2.8% lipid (dry mass based), and native CB concentrations in the range of 3-84 ng/g. Sample preparation was made by supercritical fluid extraction using pure CO2 as extraction fluid. Analysis by high-resolution gas chromatography-electron-capture detection analysis was carried out with on-column injection on two parallel coupled columns, a 60 m DB-17 column and a series combination of a 25 m SIL-8 column and a 25 m HT-5 column. Supercritical fluid extraction was compared with Soxhlet extraction and found to give quantitative recoveries, detection limits of 0.5-2 ng/g and standard deviations of less than 5% on average. The developed method was confirmed on nine different lyophilized fish samples which contained 6.1-26.5% lipid (dry mass based), and native CB concentrations in the range 0.8-134 ng/g.
authors.affiliation: Environment Institute, CEC Joint Research Centre, Ispra (VA), Italy.
authors.forename: S
authors.initials: S
authors.lastname: Bøwadt
authors.forename: B
authors.initials: B
authors.lastname: Johansson
authors.forename: P
authors.initials: P
authors.lastname: Fruekilde
authors.forename: M
authors.initials: M
authors.lastname: Hansen
authors.forename: D
authors.initials: D
authors.lastname: Zilli
authors.forename: B
authors.initials: B
authors.lastname: Larsen
authors.forename: J
authors.initials: J
authors.lastname: de Boer
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D004583:Electrons; D005399:Fishes; D005519:Food Preservation; D005612:Freeze Drying; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D013045:Species Specificity; D014413:Tuna
chemical_list: D011078:Polychlorinated Biphenyls