Synthetic Rab3A effector domain peptide stimulates inositol 1,4,5-trisphosphate production in various permeabilized cells.
Synthetic peptides corresponding to the effector domain of the small molecular weight GTP-binding protein Rab3A are known to stimulate exocytosis in various secretory cells. In the present study, we report that Rab3A effector domain peptide (33-48) causes accumulation of inositol 1,4,5-trisphosphate (1,4,5-IP3) in permeabilized pancreatic acinar cells, hepatocytes, 3T3 fibroblasts, and SH-SY5Y neuroblastoma cells. A scrambled peptide of Rab3A had no effect showing specificity of the Rab3A peptide response. No effect was observed in intact cells indicating that the target of the peptide is located intracellularly. We conclude that Rab3 effector domain peptide-induced accumulation of 1,4,5-IP3 is a wide-spread phenomenon, suggesting regulation of phosphoinositide-specific phospholipase C by Rab3-like proteins.
Piiper
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A
II. Medical Department, University of Frankfurt/M., Germany.
Stryjek-Kaminska
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D
Zeuzem
S
S
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D004072:Digitonin / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000378:metabolism; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured; D014667:Vasopressins / Q000494:pharmacology; D020692:rab3 GTP-Binding Proteins
D010446:Peptide Fragments; D014667:Vasopressins; D015544:Inositol 1,4,5-Trisphosphate; D002217:Carbachol; D019204:GTP-Binding Proteins; D020692:rab3 GTP-Binding Proteins; D004072:Digitonin; D001839:Bombesin