Expression of transcripts for CSF-1 and for the "macrophage" and "epithelial" isoforms of the CSF-1R transcripts in human ovarian carcinomas.
Levels of CSF-1 and CSF-1 receptor (CSF-1R) (fms) transcripts were quantified by Northern and dot blotting in total RNA extracts from 68 human ovarian carcinomas and positive and negative controls. Overall, very high levels of CSF-1 and CSF-1R transcripts were found in 11 and 21% of ovarian carcinoma specimens, respectively. When probes specific for CSF-1R exon1 were employed, which hybridize with the "epithelial" but not with the "hematopoietic" isoforms of the CSF-1R transcripts, the results suggested that both isoforms of the CSF-1R transcript were present in the tumor specimens. When levels of CSF-1 and CSF-1R were compared, a positive correlation was observed. Taken together, these results confirm prior observations of high levels of CSF-1 and CSF-1R in ovarian carcinoma specimens and that the latter include both "epithelial" and "macrophage" CSF-1R transcript isoforms, as might be expected for specimens composed of malignant epithelial cells and stromal macrophages.
Bauknecht
T
T
Department of Gynecology and Obstetrics, Albert-Ludwigs University, Freiburg, Germany.
Kiechle-Schwarz
M
M
du Bois
A
A
Wölfle
J
J
Kacinski
B
B
D002277:Carcinoma / Q000737:chemistry*; D004848:Epithelium; D005260:Female; D006801:Humans; D015151:Immunoblotting; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis*; D008264:Macrophages; D010051:Ovarian Neoplasms / Q000737:chemistry*; D012334:RNA, Neoplasm / Q000737:chemistry; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis*
D012334:RNA, Neoplasm; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor