Monoclonal antibodies to the carbohydrate structure Lewis(x) stimulate the adhesive activity of leukocyte integrin CD11b/CD18 (CR3, Mac-1, alpha m beta 2) on human granulocytes.
Several carbohydrate structures on human granulocytes have been discussed as potential ligands for C-type lectins (selectins) on endothelial cells. Among them are the lacto-series type II chain antigens sialyl-Lewis(x) (SLe(x), Lewis(x) (Le(x)), and VIM2. We demonstrated in this study that monoclonal antibodies (mAbs) to Le(x) and to SLe(x), but not other anticarbohydrate mAbs (VIM2, CDw17, CD24), can stimulate granulocytes to form homotypic aggregates. This effect was particularly noticeable with three distinct anti-Le(x) mAbs (3C6, 4D1, 6C7). Much less impressive effects were also seen with nine other anti-Le(x) mAbs and with the anti-SLe(x) mAb CSLEX1. Aggregation was shown to be an active process. It is temperature and energy dependent, requires divalent cations, and is selective in terms of mAb specificity. Anti-Le(x)-induced homoaggregate formation could be inhibited with CD11b mAb JML-H11 and CD54 (ICAM1) mAb LB-2 and thus seems to be associated with activation of the beta 2-integrin cytoadhesion pathway.
Stöckl
J
J
Institute of Immunology, University of Vienna, Austria.
Majdic
O
O
Rosenkranz
A
A
Fiebiger
E
E
Kniep
B
B
Stockinger
H
H
Knapp
W
W
D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology* / Q000494:pharmacology*; D000918:Antibody Specificity; D015703:Antigens, CD / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D018845:CD11 Antigens; D018821:CD18 Antigens; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006017:Glycolipids / Q000032:analysis / Q000276:immunology; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D006098:Granulocytes / Q000166:cytology* / Q000276:immunology* / Q000502:physiology; D006801:Humans; D015151:Immunoblotting; D018799:Intercellular Adhesion Molecule-1; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D016256:Lewis X Antigen / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D013696:Temperature
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D006017:Glycolipids; D006023:Glycoproteins; D016256:Lewis X Antigen; D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1