Response to glucose of transcription factor binding to the rat insulin-1 gene promoter.
The mechanism by which glucose stimulates insulin gene expression has been investigated by studying the binding of nuclear proteins to a putative glucose-sensitive element (GSE) in the rat insulin-I gene promoter. Gel retardation assays showed that a specific binding activity was present in four different beta-cell lines. The binding activity was increased by glucose only in those beta-cell lines which were shown to retain glucose-regulated insulin gene transcription. However, a similar binding activity was also shown to be present in an alpha-cell line. The protein factor binding to the GSE was estimated to have a molecular weight of 27kD. This protein may play a pivotal role in glucose-regulated transcription of the insulin gene.
Goodison
S
S
Nuffield Department of Clinical Biochemistry, University of Oxford, John Radcliffe Hospital, Headington, UK.
Miyazaki
J
J
Ashcroft
S J
SJ
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D006224:Cricetinae; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000235:genetics*; D007340:Insulinoma; D007515:Islets of Langerhans / Q000378:metabolism*; D010190:Pancreatic Neoplasms; D011384:Proinsulin / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
D007328:Insulin; D011498:Protein Precursors; D012333:RNA, Messenger; D014157:Transcription Factors; C013737:preproinsulin; D004247:DNA; D011384:Proinsulin; D015500:Chloramphenicol O-Acetyltransferase; D005947:Glucose