CD2: a functional adhesion molecule on murine B cells, involved in interleukin-4-induced aggregation.
The murine equivalent to CD2, previously known as a T cell marker, is expressed on mouse B cells. The monoclonal anti-CD2 antibody 12-15 was found to induce B cell homotypic adhesion. When treated with F(ab')2 fragments of 12-15, purified, resting B cells aggregate within 2 h of incubation and the response is optimal after 20 h. Anti-CD2-induced aggregation is a dose-related active process, dependent on temperature, metabolic energy and divalent cations. Aggregation is inhibited by two different Fab monomers of anti-CD2, implying that it is the CD2 molecule itself that functions as an adhesion molecule. We also report that interleukin 4-induced B cell homotypic adhesion involves CD2-mediated cell binding, since the antibodies specific for mouse, CD2, inhibited interleukin-4-induced cell aggregation.
Elenström-Magnusson
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C
Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
Altevogt
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Severinson
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D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D001402:B-Lymphocytes / Q000166:cytology*; D018801:CD2 Antigens; D002413:Cations, Divalent / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002449:Cell Aggregation / Q000187:drug effects; D004734:Energy Metabolism; D015847:Interleukin-4 / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D011971:Receptors, Immunologic / Q000502:physiology*; D016028:Receptors, Leukocyte-Adhesion / Q000502:physiology*; D013696:Temperature
D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D002413:Cations, Divalent; D015815:Cell Adhesion Molecules; D011971:Receptors, Immunologic; D016028:Receptors, Leukocyte-Adhesion; D015847:Interleukin-4