Role of endogenous interferon-beta in the restriction of HIV replication in human monocyte/macrophages.
In vitro culture of human monocytes results in a time-dependent differentiation into macrophages. Monocyte/macrophages were infected with HIV-1Ba-L at different times after isolation and subsequent culture. When 7-day macrophages were infected in the presence of antibodies to interferon-beta (IFN-beta), a significant increase in HIV-1 p24 release was observed. This effect was not detected in 1-day monocytes. Treatment of 7-day cultured macrophages with HIV-1 rgp120 resulted in resistance to vesicular stomatitis virus infection. This rgp120-induced antiviral state was neutralized in the presence of antibodies to IFN-beta. The overall results indicate that the infection of monocyte/macrophages with HIV-1 results in the induction of IFN-beta, which, in turn, inhibits HIV-1 expression in macrophages. The finding that HIV-1 itself (possibly through its gp120) can induce a potent antiviral factor (IFN-beta) in macrophages underlines the complex physiological function of these cells in maintaining normal homeostasis in vivo in response to virus infection.
Gessani
S
S
Department of Virology, Istituto Superiore di Sanità, Rome, Italy.
Puddu
P
P
Varano
B
B
Borghi
P
P
Conti
L
L
Fantuzzi
L
L
Gherardi
G
G
Belardelli
F
F
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000503:physiopathology; D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000494:pharmacology; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015967:Gene Expression Regulation, Viral; D015699:HIV Envelope Protein gp120 / Q000494:pharmacology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D016899:Interferon-beta / Q000276:immunology / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000382:microbiology*; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014779:Virus Replication / Q000502:physiology*
D000906:Antibodies; D004279:DNA, Viral; D015699:HIV Envelope Protein gp120; D016899:Interferon-beta