Absorption of surfactants by membranes: erythrocytes versus synthetic vesicles.
Three surfactants (chlorpromazine hydrochloride, thioridazine hydrochloride, and sodium deoxycholate) are found to absorb just as strongly into the protein-containing membranes of erythrocytes as into the phospholipid bilayers of synthetic vesicles. In the concentration region where hemolysis occurs and the Langmuir adsorption isotherm is no longer valid, one may use a phase partition model in which the erythrocyte membrane is one of the phases. The partition coefficients, expressed as the ratio of mole fraction surfactant in the membrane lipid phase to concentration of surfactant in the aqueous phase, have been calculated at the point of saturation in the erythrocyte membrane. These values are Ky = 430 M-1 (chlorpromazine, pH 5.9), 550 M-1 (deoxycholate, pH 7.6), and 640 M-1 (thioridazine, pH 5.9), in isotonic buffer at 27 degrees C. Corresponding values for synthetic vesicles made from dimyristoylphosphatidylcholine are Kx = 230 M-1 (chlorpromazine, 0.12 M buffer/KCl pH 5.9), 440 M-1 (deoxycholate, 0.20 M buffer/NaCl pH 8.0) and 510 M-1 (thioridazine, 0.12 M buffer/KCl pH 5.9), at 27 degrees C. It appears that the surfactants become an integral part of the bilayer in both vesicles and natural membranes and that the absorption is not of a peripheral nature. There is no evidence that the presence of proteins in the natural membrane inhibits the absorption of these surfactants in any way.
Binford
J S
JS
Department of Chemistry, University of South Florida, Tampa 33620.
Palm
W H
WH
D000042:Absorption; D002746:Chlorpromazine / Q000737:chemistry / Q000493:pharmacokinetics; D003840:Deoxycholic Acid / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008051:Lipid Bilayers; D008433:Mathematics; D008954:Models, Biological; D012044:Regression Analysis; D013501:Surface-Active Agents / Q000737:chemistry* / Q000493:pharmacokinetics*; D013881:Thioridazine / Q000737:chemistry / Q000493:pharmacokinetics
D008051:Lipid Bilayers; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D013881:Thioridazine; D002746:Chlorpromazine