Psoriasin binds calcium and is upregulated by calcium to levels that resemble those observed in normal skin.
Recently, we described a small molecular weight protein termed psoriasin that showed sequence similarity with the S100 calcium-binding proteins and that is highly upregulated in psoriatic epidermis as well as in primary human keratinocytes undergoing abnormal differentiation. Here we present evidence showing that natural and recombinant psoriasin binds calcium, as judged by the calcium overlay assay, and that it contains all the sequence features characteristic of the S100 family. Furthermore, [35S]-methionine labeling experiments showed that psoriasin synthesis is upregulated by 2 mM Ca++ (ratio Ca++/control at 88 h = 2.56) to levels that resemble those observed in unfractionated keratinocyte populations obtained from normal skin.
Hoffmann
H J
HJ
Institute of Medical Biochemistry, Aarhus University, Denmark.
Olsen
E
E
Etzerodt
M
M
Madsen
P
P
Thøgersen
H C
HC
Kruse
T
T
Celis
J E
JE
D000595:Amino Acid Sequence; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D012016:Reference Values; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins / Q000145:classification; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human; D014212:Tretinoin; D002118:Calcium