Localization of several G-protein subunits in human kidney.
Localization of guanine nucleotide binding protein (G-protein) alpha chains in human kidney was examined using immunoblotting and immunocytochemical techniques. Immunoblot studies revealed G alpha s, G alpha i1,2 and G alpha i3 proteins in membrane fractions of glomeruli, cortical tubules and medullary tubules. Immunocytochemical studies confirmed the presence of these G-proteins in several parts of human nephron. The results demonstrate the presence of a large amount of G-protein alpha subunits in human nephron segments. We speculate that G-protein in human kidney may play an important physiological role well-defined in other mammalia.
Ishidoya
S
S
Department of Urology, Tohoku University School of Medicine, Sendai, Japan.
Fukuzaki
A
A
Orikasa
S
S
D002462:Cell Membrane; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000737:chemistry* / Q000166:cytology; D007672:Kidney Cortex / Q000737:chemistry; D007678:Kidney Glomerulus / Q000737:chemistry; D007684:Kidney Tubules / Q000737:chemistry
D019204:GTP-Binding Proteins