Murine T-helper cell immune response to recombinant vaccinia-Venezuelan equine encephalitis virus.
The T-helper (Th) cell immune response following immunization of C3H (H-2k) mice with a recombinant vaccinia (VAC) virus (TC-5A) expressing the structural proteins (capsid, E1 and E2) of the attenuated vaccine strain (TC-83) of Venezuelan equine encephalitis (VEE) virus was compared with the immune response induced in mice after immunization with TC-83 virus. TC-5A virus elicited Th cells that strongly recognized both VAC and TC-83 viruses in in vitro lymphoblastogenesis tests. Th-cell activation was associated with elevated levels of interleukin-2. TC-5A virus induced long-term humoral immunity; VEE virus-binding and neutralizing antibodies were detected in mouse sera collected from mice 16 months after a single immunization.
Mathews
J H
JH
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522.
Kinney
R M
RM
Roehrig
J T
JT
Barrett
A D
AD
Trent
D W
DW
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology*; D007114:Immunization; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012845:Sindbis Virus / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D007376:Interleukin-2; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D014765:Viral Vaccines