title: Brain regional distribution of prion protein PrP27-30 in mice stereotaxically microinjected with different strains of scrapie.
abstract: Stereotaxic inoculation was used to examine the role of scrapie agent strain, inoculum, and injection site on the brain regional distribution of the prion protein, PrP27-30. Neither the type of inoculum nor the injection site influenced the distribution of PrP27-30 in brains of mice. Among the parameters examined, only the strain of agent affected the pattern of distribution and the yield of PrP27-30. Although mice injected into the cerebellum had the shortest incubation period, the cerebellum gave the lowest yield of the PrP27-30 among the seven brain regions examined. The positive correlation between PrP27-30 regional distribution and lesion profile (degree of vacuolation) reinforces the role of the PrPSC protein in scrapie pathogenesis.
authors.affiliation: New York State Office of Mental Retardation and Developmental Disabilities, Department of Virology, Staten Island.
authors.forename: P
authors.initials: P
authors.lastname: Casaccia-Bonnefil
authors.forename: R J
authors.initials: RJ
authors.lastname: Kascsak
authors.forename: R
authors.initials: R
authors.lastname: Fersko
authors.forename: S
authors.initials: S
authors.lastname: Callahan
authors.forename: R I
authors.initials: RI
authors.lastname: Carp
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D051379:Mice; D008845:Microinjections; D018622:PrP 27-30 Protein; D011328:Prions / Q000378:metabolism*; D012608:Scrapie / Q000378:metabolism*
chemical_list: D011328:Prions; D018622:PrP 27-30 Protein