Role of surface components in the process of interaction of Giardia duodenalis with epithelial cells in vitro.
Monosaccharides, lectins, periodate, trypsin and neuraminidase were used to analyse the process of adhesion of Giardia duodenalis trophozoites to IEC cells, an intestinal epithelial cell line. Addition of N-acetyl-glucosamine, N-acetyl-galactosamine, galactose and fucose to the interaction medium inhibited attachment of the parasites to the epithelial cells. Experiments in which the parasites or epithelial cells were treated before interaction showed that these monosaccharides interfered with both cell surfaces. Trypsin-sensitive, but not neuraminidase-sensitive, groups exposed on the cell surface are important for the parasite-epithelial cell association. Fluorescein isothiocyanate (FITC)- or colloidal gold-labeled lectins were used to analyse the distribution of carbohydrates on the surface of G. duodenalis and epithelial cells. It is important to stress here the presence of fucose on the parasite surface. Treatment of the cells with lectins was also used to analyse the role of carbohydrate-containing macromolecules in the parasite-cell interaction.
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Laboratório de Ultraesturura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
de Souza
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D000818:Animals; D002460:Cell Line; D004848:Epithelium / Q000469:parasitology; D005872:Giardia / Q000502:physiology*; D006790:Host-Parasite Interactions / Q000187:drug effects / Q000502:physiology; D007422:Intestines / Q000469:parasitology*; D037102:Lectins / Q000494:pharmacology; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009005:Monosaccharides / Q000494:pharmacology; D009439:Neuraminidase / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D037102:Lectins; D009005:Monosaccharides; D010504:Periodic Acid; C009288:metaperiodate; D009439:Neuraminidase; D014357:Trypsin