Cell surface antigen CD38 identified as ecto-enzyme of NAD glycohydrolase has hyaluronate-binding activity.
An ecto-enzyme of NAD glycohydrolase induced by retinoic acid in human leukemic HL-60 cells is attributed to the molecule of leukocyte cell surface antigen CD38 (Kontani, K., et al. (1993) J. Biol. Chem. 268, 16895-16898). The cell surface antigen has an amino acid sequence homologous to Aplysia ADP-ribosyl cyclase that catalyzes the conversion of NAD to cyclic ADP-ribose with a calcium-mobilizing activity. A putative hyaluronate (HA)-binding motif which has recently been identified in CD44 antigen existed in the extracellular domain and intracellular amino terminus of CD38 antigen. CD38 antigen was indeed capable of binding to HA in a manner dependent on ionic strength. By contrast, no binding activity was found in Aplysia ADP-ribosyl cyclase. Thus CD38 antigen, like CD44 antigen characterized as a HA-receptor (or binding) protein, may function as an adhesion molecule.
Nishina
H
H
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Inageda
K
K
Takahashi
K
K
Hoshino
S
S
Ikeda
K
K
Katada
T
T
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000378:metabolism*; D001048:Aplysia / Q000201:enzymology; D001665:Binding Sites; D002846:Chromatography, Affinity; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D017385:Sequence Homology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000943:Antigens, Differentiation; D008562:Membrane Glycoproteins; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D014212:Tretinoin; D006820:Hyaluronic Acid; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; C498155:Cd38 protein, mouse; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1