title: Determination of the amino acid sequence of apamin by tandem mass spectrometry.
abstract: A new strategy has been developed for the determination of the amino acid sequence of apamin, a two disulfide-bond neurotoxic oligopeptide. Relative molecular mass up to about 2000 was determined using electrospray mass spectrometry. This technique shows that three basic amino acid residues are present in apamin. A hydrophobic tryptic digest fragment was sequenced using fast-atom bombardment combined with tandem mass spectrometry. But the full sequence has been directly observed using a tandem mass spectrometer: the daughter-ion mass spectrum of protonated molecules of dithiothreitol-reduced apamin gives the sequence.
authors.affiliation: Laboratoire de Spectrométrie de Masse, IFRMP-INSERM-CNRS-Université de Rouen, Mont Saint Aignan, France.
authors.forename: C
authors.initials: C
authors.lastname: lange
authors.forename: L
authors.initials: L
authors.lastname: Marvin
authors.forename: A
authors.initials: A
authors.lastname: Marcual
authors.forename: N
authors.initials: N
authors.lastname: Ordsmith
authors.forename: R S
authors.initials: RS
authors.lastname: Bordoli
mesh_terms: D000595:Amino Acid Sequence; D001030:Apamin / Q000032:analysis*; D004229:Dithiothreitol; D006868:Hydrolysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014357:Trypsin
chemical_list: D001030:Apamin; D014357:Trypsin; D004229:Dithiothreitol