Differential effects of dihydrotestosterone and estrogen on the development of motoneuron morphology in a sexually dimorphic rat spinal nucleus.
The rat lumbar spinal cord contains a sexually dimorphic motor nucleus, the spinal nucleus of the bulbocavernosus (SNB), whose motoneurons innervate perineal muscles involved in copulatory reflexes. Dendritic development of SNB motoneurons is biphasic and androgen dependent. During the first 4 postnatal weeks, SNB dendrites grow exuberantly, and subsequently retract to mature lengths by 7 weeks of age. After early postnatal castration, SNB dendrites fail to grow, and testosterone replacement restores this growth. In other systems, testosterone and its metabolites, dihydrotestosterone and estrogen, are important for somatic and neural sexual differentiation. The purpose of the present study was to examine the effects of castration and dihydrotestosterone or estrogen replacement on the growth of SNB motoneuron somata and dendritic arbors. Male rat pups were castrated on postnatal (P) day 7 and treated daily with either dihydrotestosterone propionate (DHTP; 2 mg) or estradiol benzoate (EB; 100 micrograms) until P28 or P49. By using cholera toxin horseradish peroxidase (BHRP) histochemistry, the soma size, dendritic length, dendritic extent, and arbor area of BHRP-labeled SNB motoneurons were measured and analyzed. Both DHTP and EB treatment supported the initial exuberant growth of SNB dendrites through P28, but EB treatment was ineffective in maintaining mature, adult lengths at P49. The possible sites of hormone action and functional implications of these hormonal treatments are discussed.
Goldstein
L A
LA
Department of Neurology, VA Medical Center, Lexington, Kentucky 40511.
Sengelaub
D R
DR
D000818:Animals; D002369:Castration; D016922:Cellular Senescence / Q000187:drug effects; D002772:Cholera Toxin; D003712:Dendrites / Q000648:ultrastructure; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006735:Horseradish Peroxidase; D008297:Male; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D012727:Sex Characteristics; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*
C052635:cholera toxin, B subunit-horseradish peroxidase; D013196:Dihydrotestosterone; D004958:Estradiol; C030445:dihydrotestosterone propionate; D002772:Cholera Toxin; D006735:Horseradish Peroxidase