Effects of L- and D-enantiomers of N omega-nitro-arginine on NMDA-evoked striatal dopamine overflow.
The microdialysis method was utilized to study the effects of N omega-nitro-L-arginine (L-NNA) and N omega-nitro-D-arginine (D-NNA), an inhibitor of nitric oxide (NO) synthesis from L-arginine and its D-enantiomer, on spontaneous and N-methyl-D-aspartate (NMDA)-evoked overflow of striatal extracellular dopamine (DA) in unanesthetized, freely moving rats. Perfusion of 3-10 mM NMDA through the microdialysis probe resulted in a dose-dependent increase in striatal DA overflow. Perfusion of 500 microM L-NNA alone did not change the DA level significantly, but when added to 3 mM NMDA, it substantially suppressed the NMDA-evoked increase of DA. Perfusion of 500 microM D-NNA altered neither the basal nor the NMDA-evoked DA level. Data suggest that the stimulatory effect of NMDA in DA release involves activation of the L-arginine-NO pathway in the striatum.
Ishida
Y
Y
Department of Psychiatry, Miyazaki Medical College, Japan.
Yamamoto
R
R
Mitsuyama
Y
Y
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D016202:N-Methylaspartate / Q000494:pharmacology*; D019335:Nitroarginine; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar
D019335:Nitroarginine; D016202:N-Methylaspartate; D001120:Arginine; D004298:Dopamine