[The isolation and study of the characteristics of a cytopathic strain of the hepatitis A virus].
A fast-growing cytopathic isolate of human hepatitis A virus (strain MB-7) was derived from fecal samples of infected patients and adapted to growth in FRhK-4 cell culture. A positive serum standard against HAV and electron microscopy were used to demonstrate that MB-7 belonged to human hepatitis A virus. The strain MB-7 induced plaque formation in FRhK-4 under agar overlay after 10-12 days of incubation. The PCR products of gene VP1 were cloned in E. coli and its primary structure was determined. MB-7 was shown to have more homology with HAV strains isolated in the USA and China.
Maĭdaniuk
A G
AG
Tiunnikov
G I
GI
Konakova
V E
VE
Bondarenko
E P
EP
Nemtsov
Iu V
IuV
Karpovich
L G
LG
Kalashnikova
T V
TV
Mamaeva
N V
NV
Sosnovtsev
S V
SV
Chizhikov
V E
VE
D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D003588:Cytopathogenic Effect, Viral; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D006506:Hepatitis A / Q000382:microbiology; D006507:Hepatovirus / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity*; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012800:Siberia; D014776:Virus Cultivation
D000956:Antigens, Viral; D018076:DNA, Complementary; D004279:DNA, Viral
Vydelenie i izuchenie kharakteristik tsitopaticheskogo shtamma virusa gepatita A.