Dituftsin and polytuftsin induce an anti-peptide IgG response to non-immunogenic peptides in mice.
The effect of covalently attaching multiple forms of the immunomodulating tetrapeptide tuftsin to normally non-immunogenic peptides was studied in BALB/c and C57Bl/6 mice. The peptides: (NANP)3 from the Plasmodium falciparum circumsporozoite protein and peptides 136-152 and 205-213 derived from the capsid protein of foot-and-mouth disease virus were coupled to polytuftsin or dituftsin. Anti-peptide IgG titers were determined after two immunizations. All of these three non-immunogenic peptides coupled to polytuftsin or dituftsin induced anti-peptide antibody production in mice while peptides alone did not elicit IgG. In addition, a conjugate of (NANP)3 and polytuftsin with built-in glycopeptide adjuvant elicited an anti-peptide response comparable in magnitude with that of a peptide-KLH conjugate. The data suggest that when non-immunogenic peptides are synthesized in tandem with dituftsin or conjugated to polytuftsin a significant immune response to the peptides may be elicited. This approach may be employed in synthetic vaccine design.
Ivanov
B B
BB
Shemyakyn Institute of Bioorganic Chemistry, Academy of Sciences of Russia, Moscow.
Meshcheryakova
E A
EA
Andronova
T M
TM
Ivanov
V T
VT
D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000276:immunology; D002213:Capsid / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology; D011108:Polymers / Q000493:pharmacokinetics*; D015800:Protozoan Proteins / Q000276:immunology*; D014405:Tuftsin / Q000276:immunology*
D007074:Immunoglobulin G; D009842:Oligopeptides; D010455:Peptides; D011108:Polymers; D015800:Protozoan Proteins; C053968:polytuftsin; C052953:(asparaginyl-alanyl-asparaginyl-proline)3; D014405:Tuftsin