Interaction of H2O2 and dithiothreitol and the genesis of cellular damage in the perfused rat heart.
1. H2O2 (0.3 mM) causes release of creatine kinase (CK) from the perfused rat heart, but only when activated by removal of extracellular Ca2+. 2. Dithiothreitol (DTT), 2 mM, provides partial protection against this damage induced by artificially-introduced O2 radicals. 3. Paradoxically, DTT triggers CK release by H2O2 in the presence of extracellular Ca2+. The interaction of these agents in stimulating and protecting against this damage to the sarcolemma is discussed.
Daniels
S
S
Department of Environmental and Evolutionary Biology, University of Liverpool, U.K.
Duncan
C J
CJ
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology*; D004357:Drug Synergism; D005609:Free Radicals; D006321:Heart / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009206:Myocardium / Q000166:cytology / Q000473:pathology; D010100:Oxygen / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
D005609:Free Radicals; D006861:Hydrogen Peroxide; D003402:Creatine Kinase; D010100:Oxygen; D004229:Dithiothreitol