Identification of major metabolites of the catechol-O-methyltransferase inhibitor nitecapone in the rat and dog.
Metabolites of nitecapone [3-(3,4-dihydroxy-5-nitrobenzylidene)-2,4-pentanedione], a potent catechol-O-methyltransferase inhibitor with gastroprotective and antiulcerogenic effects, were isolated by extraction and HPLC from dog and rat urine after enzymatic hydrolysis and as glucuronic acid and sulfate conjugates. Eight and 10 nonconjugated metabolites and unchanged nitecapone were found in hydrolyzed dog and rat urine, respectively, and identified by HPLC with diode-array UV detection, electron ionization mass spectrometry, and IR spectroscopy. In both species the main phase I metabolic pathways were: 1) reduction of the side chain carbon-carbon double bond and carbonyl groups and 2) cleavage of the side-chain double bond, giving an aromatic aldehyde that was partly oxidized to the corresponding carboxylic acid. These phase I metabolites and unchanged nitecapone were excreted in urine mainly as their glucuronides and sulfates in both species. Additionally, the 3-O-methylated metabolite, not found in urine, was identified in rat plasma.
Wikberg
T
T
Orion-Farmos Pharmaceuticals, Orion Research Center, Espoo, Finland.
Taskinen
J
J
D000818:Animals; D065098:Catechol O-Methyltransferase Inhibitors; D002396:Catechols / Q000097:blood / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005260:Female; D005965:Glucuronates / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D008745:Methylation; D010084:Oxidation-Reduction; D010422:Pentanones / Q000097:blood / Q000378:metabolism* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000378:metabolism
D065098:Catechol O-Methyltransferase Inhibitors; D002396:Catechols; D005965:Glucuronates; D010422:Pentanones; D013431:Sulfates; C057364:nitecapone