title: Effect of alteration of charged residues at the N termini of signal peptides on protein export in Bacillus subtilis.
abstract: The role of positively charged residues at the N termini of signal peptides in protein export has been studied in Bacillus subtilis. Bacillus signal peptides (alkaline protease [Apr] and neutral protease [Npr] from Bacillus amyloliquefaciens) were altered and fused to mature levansucrase (Lvs). The effects of the various alterations on the export of Lvs in B. subtilis were determined. The replacement of positively charged residues with neutral residues in both Apr and Npr signal peptides resulted in a slight defect in the export of Lvs from B. subtilis. Introduction of a negatively charged residue (aspartic acid) at the N terminus of Npr signal peptide blocked the export of Lvs. However, Apr signal peptide with a net charge of -3 (three aspartic acid residues) was still functional.
authors.affiliation: Central Research and Development Division, E. I. duPont de Nemours and Company, Wilmington, Delaware 19880-0328.
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.forename: V
authors.initials: V
authors.lastname: Nagarajan
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000502:physiology*; D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D001412:Bacillus subtilis / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics; D006602:Hexosyltransferases / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000737:chemistry; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D021382:Protein Sorting Signals / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013381:Subtilisins / Q000737:chemistry / Q000235:genetics
chemical_list: D000596:Amino Acids; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D006602:Hexosyltransferases; C021513:levansucrase; D013381:Subtilisins; D008666:Metalloendopeptidases; C085836:Npr protease