Studies on Emory mouse cataracts: oxidative factors.
Emory mouse cataracts were analyzed for amino acids, protein carbonyls and fatty acids. The tissue membrane integrity was assessed by studying chromium-51 efflux. An effect of vitamin E-free diet on cataract progression was also studied. Chromium leakage was faster from the cataractous lenses, indicating a generalized membrane damage. This was also apparent from the loss of amino acids. The damage involves oxidation of proteins, as well as of lipids. Protein oxidation was apparent by a hydrazone formation with 2,4-dinitrophenyl hydrazine. The lipid oxidation was apparent from a decrease in oleic acid and appearance of the corresponding ketoacids. Lipid oxidation was also apparent by an attenuating effect of vitamin E.
Varma
S D
SD
University of Maryland, School of Medicine, Baltimore 21201.
Devamanoharan
P S
PS
Mansour
S
S
Teter
B
B
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002386:Cataract / Q000235:genetics / Q000378:metabolism* / Q000517:prevention & control; D002463:Cell Membrane Permeability; D002860:Chromium Radioisotopes; D003459:Crystallins / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D007908:Lens, Crystalline / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010084:Oxidation-Reduction
D000596:Amino Acids; D002860:Chromium Radioisotopes; D003459:Crystallins; D005227:Fatty Acids