title: [A comparative evaluation of an immunoenzyme analytical method using a fluorogenic or chromogenic substrate in determining the Marburg virus antigen].
abstract: A comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of Marburg virus antigen was carried out. The resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-8) g of protein for the latter substrate. Cross titrations demonstrated the lack of common antigens with Ebola virus.
authors.forename: V A
authors.initials: VA
authors.lastname: Kutuzov
authors.forename: V V
authors.initials: VV
authors.lastname: Mikhaĭlov
authors.forename: S E
authors.initials: SE
authors.lastname: Kruglova
authors.forename: V V
authors.initials: VV
authors.lastname: Kutuzov
authors.forename: A P
authors.initials: AP
authors.lastname: Kirillov
authors.forename: V P
authors.initials: VP
authors.lastname: Krasnianskiĭ
authors.forename: V A
authors.initials: VA
authors.lastname: Pshenichnov
authors.forename: E V
authors.initials: EV
authors.lastname: Lebedinskaia
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D029043:Ebolavirus / Q000276:immunology; D005069:Evaluation Studies as Topic; D006923:Hymecromone / Q000031:analogs & derivatives; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D029024:Marburgvirus / Q000276:immunology*; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D011817:Rabbits
chemical_list: D000956:Antigens, Viral; D007202:Indicators and Reagents; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; C005359:4-methylumbelliferyl phosphate; D006923:Hymecromone
vernacular_title: Sravnitel'naia otsenka metoda immunofermentnogo analiza s primeneniem fliuorogennogo ili khromogennogo substrata pri opredelenii antigena virusa Marburg.