title: The unfolding and attempted refolding of the bacterial chaperone protein groEL (cpn60).
abstract: The unfolding of the bacterial chaperone protein groEL (cpn60) in solutions of guanidinium chloride (GdnHCl) has been studied. From the results of CD, fluorescence and light scattering, it is clear that major structural transitions in the protein occur over the range 1.0-1.5 M GdnHCl. The ATPase activity of the protein is lost at lower concentrations (0.75 M). After denaturation in concentrations of GdnHCl above 1.5 M, removal of the denaturing agent by dialysis results in very nearly complete regain of secondary structure (as judged by CD), but not the regain of correct tertiary or quaternary structure, or ATPase activity. The product was shown to be very sensitive to proteolysis by thermolysin, unlike the native protein, and not to show enhanced binding of ANS, a characteristic property of the 'molten globule' state of proteins. The results are discussed in relation to current information concerning the assembly of the groEL protein.
authors.affiliation: Department of Biological and Molecular Sciences, University of Stirling, UK.
authors.forename: N C
authors.initials: NC
authors.lastname: Price
authors.forename: S M
authors.initials: SM
authors.lastname: Kelly
authors.forename: G J
authors.initials: GJ
authors.lastname: Thomson
authors.forename: J R
authors.initials: JR
authors.lastname: Coggins
authors.forename: S
authors.initials: S
authors.lastname: Wood
authors.forename: A
authors.initials: A
authors.lastname: auf der Mauer
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D018834:Chaperonin 60; D002942:Circular Dichroism; D019791:Guanidine; D006146:Guanidines; D006360:Heat-Shock Proteins / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet; D013820:Thermolysin
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D006146:Guanidines; D006360:Heat-Shock Proteins; D013820:Thermolysin; D000251:Adenosine Triphosphatases; D019791:Guanidine