Application of polymer-protected ultrafine platinum particles to the immunological detection of human serum albumin.
The immunological properties of ultrafine platinum particles protected with poly(methyl acrylate)-co-N-vinyl-2-pyrrolidone, having a group chemically reactive with protein, were examined. Polymer-protected ultrafine particles were able to chemically bond multiple human serum albumin (HSA) molecules on their surface. The particles behaved as a multi-epitope-type antigen for the immunoreaction. In an ELISA, the HSA-bound particles and HSA molecules competitively reacted against the anti-HSA monoclonal antibodies. The limit of detection of HSA was ca. 10 ng/ml in the assay. The particles were used in an immunodot analysis of anti-HSA polyclonal antibody. Within 30 min of the reaction, 1-10 micrograms/ml of HSA was detected. The protein fixed polymer-protected ultrafine particles are a useful new material in immunochemistry.
Uda
T
T
Hiroshima Prefectural University, Shoubara City, Japan.
Kanmei
R
R
Akasofu
S
S
Hifumi
E
E
Ohtaki
M
M
D000818:Animals; D000941:Antigens / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010316:Particle Size; D010984:Platinum; D011109:Polymethacrylic Acids; D011485:Protein Binding; D011760:Pyrrolidinones; D012709:Serum Albumin / Q000032:analysis* / Q000378:metabolism
D000941:Antigens; D011109:Polymethacrylic Acids; D011760:Pyrrolidinones; D012709:Serum Albumin; C030613:polymethacrylic acid; D010984:Platinum; C042670:N-vinyl-2-pyrrolidinone