title: Cytokinin regulation of a soybean pollen allergen gene.
abstract: Cytokinin treatment of suspension-cultured soybean cells stimulated the accumulation of an mRNA, called cim 1, by a factor of ca. 20 within 4 h. Induction of cim 1 mRNA accumulation occurred at benzyladenine concentrations as low as 10(-8) M. Furthermore, cim 1 mRNA accumulation was stimulated in the absence of cytokinin by staurosporine (an inhibitor of protein kinases) and inhibited in the presence of cytokinin by okadaic acid (an inhibitor of protein phosphatases 1 and 2a), suggesting that cim 1 accumulation in response to cytokinin is dependent on cytokinin-induced dephosphorylation of one or more cellular proteins. The deduced amino acid sequence of the cim 1 protein product, derived from the complete nucleotide sequence of a cim 1 cDNA, was 40% identical to that of a perennial rye grass pollen allergen cDNA (Lol Pl). This sequence also indicated that the cim 1 protein product contains a putative signal peptide followed by predominantly hydrophilic residues, consistent with the hypothesis that it is exported to the apoplast.
authors.affiliation: Department of Biology, Indiana University-Purdue University at Indianapolis 46202-5132.
authors.forename: D N
authors.initials: DN
authors.lastname: Crowell
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D000485:Allergens / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D002478:Cells, Cultured; D003583:Cytokinins / Q000494:pharmacology; D004988:Ethers, Cyclic / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D011058:Pollen / Q000235:genetics* / Q000276:immunology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D030262:Soybean Proteins; D013025:Glycine max / Q000166:cytology / Q000235:genetics* / Q000276:immunology; D019311:Staurosporine
chemical_list: D000470:Alkaloids; D000485:Allergens; D052179:Antigens, Plant; D003583:Cytokinins; D004988:Ethers, Cyclic; C042814:Lol p I protein, Lolium perenne; D010940:Plant Proteins; D012333:RNA, Messenger; D030262:Soybean Proteins; C089150:cim 1 protein, soybean; D019319:Okadaic Acid; D011493:Protein Kinase C; D010749:Phosphoprotein Phosphatases; D019311:Staurosporine