DNA HLA-DR typing results of 4000 kidney transplants.
Recipients (4076) and donors (3325) of kidney transplants performed at 110 transplant centers were typed for HLA-DRB by the DNA RFLP method. The discrepancy rate of replicate samples distributed among 8 participating laboratories was a low 2.6%. The discrepancy rate between RFLP-DRB and serological HLA-DR typings was 25.0% for organ donors and 27.6% for kidney recipients. Discrepancy rates at the different transplant centers ranged from 9.7% to 86.7%. The discrepancies consisted of antigens being incorrectly interpreted by serology (16.8%), and of serological "blanks" turning out to be definable alleles by the DNA method (10.8%). The alleles that were mainly affected by discrepancies were DR1, DR8, DR10, DR12, DR13, DR14, DR16, DR17.2, and DR18.
Mytilineos
J
J
Transplant Laboratory, University of Heidelberg, Germany.
Scherer
S
S
Dunckley
H
H
Trejaut
J
J
Chapman
J
J
Fischer
G
G
Fae
I
I
Middleton
D
D
Savage
D
D
Bignon
J D
JD
D000483:Alleles; D004247:DNA / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis* / Q000235:genetics; D006650:Histocompatibility Testing; D006720:Homozygote; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D012150:Polymorphism, Restriction Fragment Length; D011786:Quality Control
D006684:HLA-DR Antigens; D004247:DNA