Leukocyte recruitment by monocyte chemotactic proteins (MCPs) secreted by human phagocytes.
Phagocyte recruitment is an important immunological phenomenon in inflammation and cancer. A large family of selective chemotactic cytokines, designated chemokines, has recently emerged. Interleukin-8 (IL-8) is the prototype of such neutrophil activating factors, whereas MCP-1 is a well studied monocyte chemotactic protein. In vitro chemotactic assays were used to isolate and identify natural chemokines from mononuclear phagocytes and tumor cells. Additional new chemotactic proteins (MCP-2, MCP-3) attracting monocytes were also discovered by these methods. All chemokines are structurally related and show affinity for heparin. MCP-1, -2 and -3 have a comparable specific activity in monocyte chemotaxis assays. Specific and sensitive radioimmunoassays for MCP-1 and IL-8 were developed to study the regulation of their secretion by leukocytes. Monocytes or monocyte tumor cells produce MCP-1 and/or IL-8 in response to cytokines, virus, double stranded RNA, bacterial endotoxin, mitogen or phorbol ester. Granulocytes were found to secrete only minor amounts of MCP-1 and IL-8.
Wuyts
A
A
Laboratory of Molecular Immunology, Rega Institute, University of Leuven, Belgium.
Proost
P
P
Put
W
W
Lenaerts
J P
JP
Paemen
L
L
van Damme
J
J
D000595:Amino Acid Sequence; D002630:Chemotactic Factors / Q000302:isolation & purification* / Q000502:physiology*; D002634:Chemotaxis, Leukocyte; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008969:Molecular Sequence Data; D018945:Monocyte Chemoattractant Proteins; D009000:Monocytes / Q000502:physiology*; D005810:Multigene Family; D009504:Neutrophils / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D002630:Chemotactic Factors; D016209:Interleukin-8; D018945:Monocyte Chemoattractant Proteins