title: Expression of a novel human myotonin protein kinase (MtPK) cDNA clone which encodes a protein with a thymopoietin-like domain in COS cells.
abstract: A full-length cDNA of human myotonin protein kinase (MtPK) was cloned and expressed in COS-1 cells. MtPK is recovered from the cytosolic fraction of the COS extract as a 70 kDa protein, which coincides with the size deduced from the predicted amino acid sequence. The sequence has a significant homology to thymopoietin, a peptide hormone of the thymus. Biochemical characteristics of MtPK expressed in COS-1 cells and its expression in rat tissues are investigated.
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sasagawa
authors.forename: H
authors.initials: H
authors.lastname: Sorimachi
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: K
authors.initials: K
authors.lastname: Arahata
authors.forename: S
authors.initials: S
authors.lastname: Ishiura
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D065636:Myotonin-Protein Kinase; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013946:Thymopoietins / Q000737:chemistry*
chemical_list: C585517:DMPK protein, human; D018076:DNA, Complementary; D013946:Thymopoietins; D011494:Protein Kinases; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases