Phylogenetic relationships of Bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit genes.
Comparative sequence analyses were performed on 14 genes encoding bacterial elongation factors EF-Tu and 7 genes encoding the beta-subunit of bacterial F1F0 type ATP-synthases. The corresponding predicted amino acid sequences were compared with published primary structures of homologous molecules. Phylogenetic trees were reconstructed from both data sets of aligned protein sequences and from an equivalent selection of 16S rRNA sequences by applying distance matrix and maximum parsimony methods. The EF-Tu data were in very good agreement with the rRNA data, although the resolution within the EF-Tu tree was reduced at certain phylogenetic levels. The resolution power of the ATPase beta-subunit sequence data were more reduced than those of the EF-Tu data. In comparison with the 16S rRNA tree there are minor differences in the order of adjacent branchings within the ATPase beta-subunit tree.
Ludwig
W
W
Lehrstuhl für Mikrobiologie, Technische Universität München, Germany.
Neumaier
J
J
Klugbauer
N
N
Brockmann
E
E
Roller
C
C
Jilg
S
S
Reetz
K
K
Schachtner
I
I
Ludvigsen
A
A
Bachleitner
M
M
D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D006180:Proton-Translocating ATPases / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D046911:Macromolecular Substances; D010444:Peptide Elongation Factor Tu; D006180:Proton-Translocating ATPases