title: Cerebral blood flow and ischemia-induced neurotransmitter release in the striatum of aged spontaneously hypertensive rats.
abstract: OBJECTIVE
  We found age-related vulnerability to cerebral ischemia in the hippocampus and striatum in spontaneously hypertensive rats. Further study revealed that ischemia-induced release of hippocampal taurine, an inhibitory amino acid, was reduced by 40% in aged rats compared with adult rats, which suggested an impaired inhibitory function against excitotoxicity in aged rats. The purpose of this study was to examine whether ischemia-induced neurotransmitter release is altered in the striatum of aged spontaneously hypertensive rats.
  
  
  METHODS
  Five adult (5-6 months) and five aged (18-22 months) female spontaneously hypertensive rats were subjected to 20 minutes of cerebral ischemia induced by bilateral carotid artery occlusions and 120 minutes of recirculation under amobarbital anesthesia (100 mg/kg i.p.). Cerebral blood flow was determined using the hydrogen clearance method, and extracellular concentrations of neurotransmitters were determined with the brain dialysis technique in the striatum.
  
  
  RESULTS
  During ischemia, cerebral blood flow in aged rats decreased to 8.7 +/- 1.2 (mean +/- SEM) mL/100 g per minute (11% of the resting), which was not different from 5.2 +/- 1.7 mL/100 g per minute (8% of the resting) in adult rats, and extracellular dopamine and amino acids (glutamate, aspartate, and taurine) increased by approximately 170- and 10-30-fold, respectively, and returned to baseline after 20-40 minutes of recirculation. These values of neurotransmitters, however, were not different between aged and adult rats during ischemia and reperfusion.
  
  
  CONCLUSIONS
  It is unlikely that a presynaptic mechanism is involved in age-related vulnerability in the striatum of hypertensive rats.
authors.affiliation: Second Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yao
authors.forename: H
authors.initials: H
authors.lastname: Ooboshi
authors.forename: S
authors.initials: S
authors.lastname: Ibayashi
authors.forename: H
authors.initials: H
authors.lastname: Uchimura
authors.forename: M
authors.initials: M
authors.lastname: Fujishima
mesh_terms: D000375:Aging / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002560:Cerebrovascular Circulation; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D000596:Amino Acids; D018377:Neurotransmitter Agents; D004298:Dopamine