title: Physiological properties of neuronal nicotinic receptors reconstituted from the vertebrate beta 2 subunit and Drosophila alpha subunits.
abstract: Three cDNAs (ALS, D alpha 2 and ARD) isolated from the nervous system of Drosophila and encoding putative nicotinic acetylcholine receptor subunits were expressed in Xenopus oocytes in order to study their functional properties. Functional receptors could not be reconstituted from any of these subunits taken singly or in twos and threes. In contrast, large evoked currents (in the microA range) were consistently observed upon agonist application on oocytes co-injected with ALS or D alpha 2 in combination with the chick beta 2 structural subunit. The ALS/beta 2 and D alpha 2/beta 2 receptors are highly sensitive to acetylcholine and nicotine, and their physiological properties resemble those of native or reconstituted receptors from vertebrates. Although the physiological properties of ALS/beta 2 and D alpha 2/beta 2 receptors are quite similar, clear differences appear in their pharmacological profiles. The ALS/beta 2 receptor is highly sensitive to alpha-bungarotoxin while the D alpha 2/beta 2 receptor is totally insensitive to this agent. These results demonstrate that the Drosophila ALS and D alpha 2 cDNAs encode neuronal nicotinic subunits responding to physiological concentrations of the agonists acetylcholine and nicotine.
authors.affiliation: Department of Physiology, CMU, Geneva, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Bertrand
authors.forename: M
authors.initials: M
authors.lastname: Ballivet
authors.forename: M
authors.initials: M
authors.lastname: Gomez
authors.forename: S
authors.initials: S
authors.lastname: Bertrand
authors.forename: B
authors.initials: B
authors.lastname: Phannavong
authors.forename: E D
authors.initials: ED
authors.lastname: Gundelfinger
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000235:genetics; D017344:Genes, Insect; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000737:chemistry*; D009538:Nicotine / Q000494:pharmacology; D009865:Oocytes; D011487:Protein Conformation; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D014981:Xenopus
chemical_list: D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D011978:Receptors, Nicotinic; D011993:Recombinant Fusion Proteins; D009538:Nicotine