title: Basic fibroblast growth factor improves recovery after chemically induced breakdown of myelin-like membranes in pure oligodendrocyte cultures.
abstract: The putative role of growth factors in remyelination was investigated in pure oligodendrocyte (OL) secondary cultures derived from newborn rat brain. These cells form myelin-like membranes and were used as a model system for toxic attack. A 24 hr treatment with 2.10(-5) M lysophosphatidylcholine (LPC) induced a loss of 59% of the cells in these cultures, with a 64% reduction in [125I]-iododeoxyuridine incorporation compared to untreated controls. An absence of processes and myelin-like sheaths was observed in the remaining cells. Numerous intracytoplasmic inclusions were observed on transmission electron microscopy. Immunocytochemical studies with A2B5 monoclonal antibody (mAb), which recognizes oligodendrocyte-type 2 astrocyte (O-2A) precursors, OL-1 mAb (directed against cell surface sulfatides), and anti-myelin basic protein (anti-MBP) antibody showed that the entire OL lineage was affected at all stages of maturation. A 3 day treatment with 10 ng/ml basic fibroblast growth factor (bFGF) induced reconstruction of myelin-like membranes, albeit less compacted than in untreated controls. The doubling in number of cells and the 46% increase in [125I]-iododeoxyuridine incorporation was due essentially to proliferation of O-2A progenitors. These results indicate that if bFGF release occurs during demyelination, it may participate in myelin repair mechanisms in the central nervous system.
authors.affiliation: Neurology Department, University Hospital, Limoges, France.
authors.forename: C
authors.initials: C
authors.lastname: Fressinaud
authors.forename: J M
authors.initials: JM
authors.lastname: Vallat
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine / Q000378:metabolism; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007065:Idoxuridine / Q000378:metabolism; D007150:Immunohistochemistry; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000187:drug effects* / Q000648:ultrastructure; D009836:Oligodendroglia / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats
chemical_list: D008244:Lysophosphatidylcholines; D016222:Fibroblast Growth Factor 2; D001973:Bromodeoxyuridine; D007065:Idoxuridine