Pavlovian conditioning alters cortical microtubule-associated protein-2.
Three experiments indicate that Pavlovian conditioning to tone alters microtubule-associated protein-2 (MAP-2) in the temporal cortex. First, increased MAP-2 immunohistochemistry was evident in temporal cortex following tone-shock pairings but not light-shock pairings. In the second experiment, animals given tone paired with shock (compared with animals trained with tone unpaired with shock or given tone only) showed MAP-2 immunohistochemical changes in the temporal cortex, as well as in the frontal and cingulate cortex, the hippocampus and amygdala. In experiment 3, quantitative immunoblots showed decreased intact MAP-2 and increased breakdown products selectively in temporal cortex following fear conditioning to tone. Conditioning to tone also increased sizes of MAP-2 rich pyramidal somata and apical dendrites in temporal and frontal cortex.
Woolf
N J
NJ
Laboratory of Chemical Neuroanatomy, University of California, Los Angeles 90024-1563.
Young
S L
SL
Johnson
G V
GV
Fanselow
M S
MS
D000161:Acoustic Stimulation; D000818:Animals; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000378:metabolism*; D003214:Conditioning, Classical / Q000502:physiology*; D004597:Electroshock; D005260:Female; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D017966:Pyramidal Cells / Q000276:immunology / Q000378:metabolism; D051381:Rats
D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins