Detection of human alpha-herpesvirus DNA using consensus primers and specific probes.
A simple and rapid diagnostic assay system was developed for detecting and identifying human alpha-herpesviruses, such as herpes simplex virus types 1 and 2 and varicella-zoster virus, by polymerase chain reaction. This system was based on an amplification step, using primers that bind the DNA polymerase consensus sequence of alpha-human herpesviruses, and a detection step, using non-radioactive virus-specific probes. This method could be used to amplify any human alpha-herpesviruses, and each virus-specific probe was highly specific for identification of the amplified product. This system is readily applicable for implementation in the clinical laboratory.
Aono
T
T
Department of Virology, Osaka University, Japan.
Murakami
S
S
Yanagihara
N
N
Yamanishi
K
K
D003587:Cytomegalovirus / Q000235:genetics; D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005784:Gene Amplification; D016697:Herpes Zoster Oticus / Q000235:genetics / Q000382:microbiology; D018259:Herpesvirus 1, Human / Q000235:genetics*; D018258:Herpesvirus 2, Human / Q000235:genetics*; D014645:Herpesvirus 3, Human / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics; D015654:Herpesvirus 6, Human / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral