Cloning and characterization of a full-length cDNA coding for ovine aldolase B from fetal mesonephros.
An ovine aldolase B cDNA was isolated from mesonephros (29 d pc). The sequence covers 1649 nucleotides. Comparison with human liver aldolase B cDNA shows a homology of about 86%. The deduced amino acid sequence is composed of 364 residues and exhibits 92% homology to the human protein. Northern blot analysis and in situ hybridization data show that during the first third of gestation in sheep, aldolase B expression is restricted to the mesonephros.
Gianquinto
L
L
Laboratoire de Biologie Cellulaire et Moléculaire, Bâtiment des Biotechnologies, INRA, Jouy-en-Josas, France.
Pailhoux
E A
EA
Bezard
J
J
Servel
N
N
Kirszenbaum
M
M
Cotinot
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D006066:Gonads / Q000502:physiology; D017403:In Situ Hybridization; D008650:Mesonephros / Q000201:enzymology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000196:embryology / Q000235:genetics*; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; D005634:Fructose-Bisphosphate Aldolase