Characterization of a novel binding site for the human immunodeficiency virus type 1 envelope protein gp120 on human fetal astrocytes.
125I-labeled recombinant gp120 bound to primary cultures of human fetal astrocytes with a single class of 260-kDa binding sites, with a Kd of 26 nM and maximal number of binding sites of 29.9 fmol/4 x 10(4) cells. Neither CD4 nor galactocerebroside was detectable on astrocytes, and 125I-labeled recombinant gp120 binding to astrocytes was not blocked by antibodies against galactocerebroside or the gp120 binding domain of CD4.
Ma
M
M
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
Geiger
J D
JD
Nath
A
A
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001253:Astrocytes / Q000378:metabolism* / Q000382:microbiology; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D015704:CD4 Antigens / Q000032:analysis / Q000276:immunology; D002462:Cell Membrane / Q000378:metabolism / Q000382:microbiology; D002478:Cells, Cultured; D005333:Fetus; D005434:Flow Cytometry; D005699:Galactosylceramides / Q000032:analysis / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D007700:Kinetics; D011991:Receptors, Virus / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D005699:Galactosylceramides; D015699:HIV Envelope Protein gp120; D007457:Iodine Radioisotopes; D011991:Receptors, Virus; D011994:Recombinant Proteins