House dust mite-responsive human T cells require both interleukin 2 (IL2) and interleukin 4 for optimal proliferation, whereas IL2 alone is sufficient for proliferation of tetanus toxoid-responsive T cells.
Production of interleukin 4 (IL4) and response to IL4 may be a key determinant of the atopic immune response. Proliferation requirements of allergen-specific T cells derived from atopics and nonatopics were determined. Peripheral blood lymphocytes were cultured with either house dust mite (HDM) or tetanus toxoid under limiting dilution conditions with interleukin 2 (IL2) alone or IL2+IL4. The relative proportion of antigen-specific T cells responding under each of these conditions was determined by proliferation. The combination of IL2+IL4 resulted in the highest proportion of responding HDM-specific T cells. Similar results were obtained with HDM-specific T cells from both atopic and nonatopic donors. These HDM and Der pI responsive cells were found to be CD4+CD8-. In contrast, tetanus toxoid-responsive T cells from both atopic and nonatopic donors grew preferentially in response to IL2 alone.
Michael
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BN
Department of Dermatology, SUNY at Stony Brook 11794-8165.
Kalish
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RS
D000328:Adult; D000485:Allergens / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides; D002455:Cell Division; D002460:Cell Line; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000276:immunology*; D015847:Interleukin-4 / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008925:Mites / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*
D000485:Allergens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D007376:Interleukin-2; D013745:Tetanus Toxoid; D015847:Interleukin-4