title: Binding of a soluble alpha beta T-cell receptor to superantigen/major histocompatibility complex ligands.
abstract: The genes for the alpha and beta chains of a murine T-cell receptor were truncated just prior to the portions encoding the transmembrane regions and introduced into baculovirus by recombination. Insect cells infected with the virus secreted a soluble form of the receptor that could be purified to homogeneity. This soluble receptor reacted with a set of six monoclonal antibodies originally raised to different epitopes on the natural transmembrane-region-containing receptor and bound with appropriate specificity to a cell surface complex of the human major histocompatibility complex class II molecule DR1 with the bacterial superantigen staphylococcal enterotoxin B.
authors.affiliation: Howard Hughes Medical Institute, Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
authors.forename: J
authors.initials: J
authors.lastname: Kappler
authors.forename: J
authors.initials: J
authors.lastname: White
authors.forename: H
authors.initials: H
authors.lastname: Kozono
authors.forename: J
authors.initials: J
authors.lastname: Clements
authors.forename: P
authors.initials: P
authors.lastname: Marrack
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D003001:Cloning, Molecular; D004768:Enterotoxins / Q000378:metabolism; D066298:In Vitro Techniques; D008024:Ligands; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009036:Moths; D011485:Protein Binding; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins; D012995:Solubility; D013210:Staphylococcus / Q000276:immunology; D018089:Superantigens / Q000378:metabolism*
chemical_list: D004768:Enterotoxins; D008024:Ligands; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011994:Recombinant Proteins; D018089:Superantigens; C031818:enterotoxin B, staphylococcal