A long range restriction map spanning the myxoid liposarcoma breakpoint in the q13-14 region of human chromosome 12.
We have used pulsed-field gel electrophoresis to construct a long range restriction map of the myxoid liposarcoma (MLS) breakpoint region in 12q13-14. The CHOP/GADD153 gene, consistently translocated in myxoid liposarcomas, is located less than 55 kb from the putative oncogene GLI. We have used fluorescent in situ hybridization to orient the map with respect to the chromosome, and to show that GLI (and thus A2MR) is located proximal to the MLS breakpoint.
Forus
A
A
Department of Tumor Biology, Norwegian Radium Hospital, Oslo.
Kools
P F
PF
Schoenmakers
E F
EF
Van de Ven
W J
WJ
Myklebost
O
O
D022762:CCAAT-Enhancer-Binding Proteins; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002881:Chromosomes, Human, Pair 12; D004273:DNA, Neoplasm / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018208:Liposarcoma, Myxoid / Q000235:genetics*; D009857:Oncogenes; D011506:Proteins / Q000235:genetics; D015183:Restriction Mapping; D051742:Transcription Factor CHOP; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
D022762:CCAAT-Enhancer-Binding Proteins; C497249:DDIT3 protein, human; D004273:DNA, Neoplasm; D011506:Proteins; D014157:Transcription Factors; D051742:Transcription Factor CHOP