title: Localization of the VirA domain involved in acetosyringone-mediated vir gene induction in Agrobacterium tumefaciens.
abstract: The VirA protein of Agrobacterium tumefaciens is thought to be a receptor for plant phenolic compounds such as acetosyringone. Although it is not known whether the interaction between VirA and the phenolics is direct or requires other phenolic-binding proteins, it is shown in this study that the first 280 amino acids of the VirA protein are not essential for the acetosyringone mediated vir gene induction response. Considering the fact that the cytoplasmic region between the amino acids 283 and 304 is highly conserved between the different VirA proteins, and that deletion of this region abolishes VirA activity, we suggest that the acetosyringone receptor domain is located in this cytoplasmic domain of the VirA protein.
authors.affiliation: Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Netherlands.
authors.forename: S C
authors.initials: SC
authors.lastname: Turk
authors.forename: R P
authors.initials: RP
authors.lastname: van Lange
authors.forename: T J
authors.initials: TJ
authors.lastname: Regensburg-Tuïnk
authors.forename: P J
authors.initials: PJ
authors.lastname: Hooykaas
mesh_terms: D000098:Acetophenones / Q000494:pharmacology*; D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000472:pathogenicity*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002628:Chemoreceptor Cells; D004252:DNA Mutational Analysis; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics; D013329:Structure-Activity Relationship; D015533:Transcriptional Activation; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors
chemical_list: D000098:Acetophenones; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C057331:Tar protein, E coli; D037521:Virulence Factors; C051667:acetosyringone