Direct 1H NMR evidence for conversion of beta-D-cellobiose to cellobionolactone by cellobiose dehydrogenase from Phanerochaete chrysosporium.
The alpha- and beta-anomers of D-cellobiose were resolved by 1H NMR spectroscopy. Addition of cellobiose dehydrogenase purified from the white-rot P. chrysosporium led to selective conversion of beta-D-cellobiose. The product was identical to cellobionolactone as synthesized from Ca-cellobionate. Overnight incubation of the product led to an altered NMR spectrum, which was also obtained by incubation of cellobionolactone. The new spectrum matched that for Ca-cellobionate. The instability of cellobionolactone explains the detection of cellobionic acid as product in earlier studies.
Higham
C W
CW
Department of Biochemistry, University of Bristol, UK.
Gordon-Smith
D
D
Dempsey
C E
CE
Wood
P M
PM
D001487:Basidiomycota / Q000201:enzymology*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002475:Cellobiose / Q000031:analogs & derivatives* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011522:Protons
D011522:Protons; D002475:Cellobiose; C089182:cellobionolactone; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase