Detection and cloning of human papillomavirus DNA associated with recurrent respiratory papillomatosis in Thailand.
Recurrent respiratory papillomatosis (RRP) is highly prevalent in Thailand. In this study, we examined the presence of human papillomavirus (HPV) DNA in 25 RRP patients in Thailand by means of dot blot analysis and/or polymerase chain reaction. Eighty-four percent (21/25) of cases and 4% (1/25) of cases were positive for HPV-11 DNA and HPV-6 DNA, respectively. Three cases (3/25) were negative for all of the examined HPV types. No cases were positive for HPV-16 or 18. Furthermore, we isolated the recombinant HPV-11 DNA clone from a genomic library constructed with the DNA of RRP tissue. The restriction map of the cloned HPV DNA was identical with the map of known HPV-11 DNA. These results suggest at least that no specific HPV type or subtype is likely to be associated with RRP in Thailand.
Ushikai
M
M
Department of Otolaryngology, Faculty of Medicine, Kagoshima University.
Fujiyoshi
T
T
Kono
M
M
Antrasena
S
S
Oda
H
H
Yoshida
H
H
Fukuda
K
K
Furuta
S
S
Hakura
A
A
Sonoda
S
S
D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D009364:Neoplasm Recurrence, Local; D009693:Nucleic Acid Hybridization; D010212:Papilloma / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections; D016133:Polymerase Chain Reaction; D012142:Respiratory Tract Neoplasms / Q000821:virology*; D015183:Restriction Mapping; D013785:Thailand; D014412:Tumor Virus Infections
D004279:DNA, Viral