Recombinant rabbit hemorrhagic disease virus capsid protein expressed in baculovirus self-assembles into viruslike particles and induces protection.
VP60, the unique component of rabbit hemorrhagic disease virus capsid, was expressed in the baculovirus system. The recombinant VP60, released in the supernatant of infected insect cells, assembled without the need of any other viral component to form viruslike particles (VLPs), structurally and immunologically indistinguishable from the rabbit hemorrhagic disease virion. Intramuscular vaccination of rabbits with the VLPs conferred complete protection in 15 days; this protection was found to be effective from the fifth day after VLP injection and was accompanied by a strong humoral response.
Laurent
S
S
Unité de Virologie et d'Immunologie Moléculaires, Institut National de la Recherche Agronomique, Jouy en Josas, France.
Vautherot
J F
JF
Madelaine
M F
MF
Le Gall
G
G
Rasschaert
D
D
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000382:microbiology / Q000473:pathology*; D002213:Capsid / Q000096:biosynthesis* / Q000276:immunology; D002460:Cell Line; D005822:Genetic Vectors; D017929:Hemorrhagic Disease Virus, Rabbit / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D008099:Liver / Q000382:microbiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009036:Moths; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology; D014771:Virion / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure
D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines