Cloning of the macrolide antibiotic biosynthesis gene acyA, which encodes 3-O-acyltransferase, from Streptomyces thermotolerans and its use for direct fermentative production of a hybrid macrolide antibiotic.
A gene encoding the macrolide modification enzyme 3-O-acyltransferase (acyA) was cloned by chromosome walking onto the carbomycin biosynthetic region in Streptomyces thermotolerans TH475, with the 3' region of the gene encoding the macrolide modification enzyme 4"-O-acyltransferase (acyB1) as a probe. A shortened fragment (1.8 kb) containing acyA was subcloned with pIJ350. A high-level tylosin producer, Streptomyces fradiae MBBF, transformed with the plasmid could produce a hybrid macrolide, 3-O-acetyltylosin, most efficiently.
Arisawa
A
A
Mercian Corporation, Central Research Laboratories, Kanagawa, Japan.
Kawamura
N
N
Takeda
K
K
Tsunekawa
H
H
Okamura
K
K
Okamoto
R
R
D000217:Acyltransferases / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D005798:Genes, Bacterial; D007933:Leucomycins / Q000737:chemistry; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*; D015645:Tylosin / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000737:chemistry
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D007933:Leucomycins; C059423:carbomycin; C031783:3-acetyl-4''-isovaleryltylosin; D000217:Acyltransferases; D015645:Tylosin