title: Differential effects of IFN-gamma on kidney cell expression of MHC class II molecules, kidney cell associated molecules and their stimulatory capacity in mixed lymphocyte kidney cell culture.
abstract: Mixed cell co-cultures of lymphocytes responding to kidney cells (MLKC), islets of Langerhans (MLIC) and mixed lymphocyte culture (MLC), were used to clarify mechanisms in allogeneic and autoimmune (tissue-associated) antigen presentation. Fresh kidney cortical tubular cells (KC), Madin-Darby canine kidney (MDCK), and dog kidney (6247) (DK) cell lines were used in MLKC reactions, and islets of Langerhans were used in the MLIC as putative antigen presenting cells (APC). The stimulating cells were treated with purified or recombinant dog interferon-gamma (IFN-gamma), and the detection of class II MHC molecule expression was assessed by a moneclonal antibody (mAb) (B1F6). Transcription of MHC class II mRNA and IFN-gamma mRNA was measured by semiquantitative polymerase chain reaction, and detection of a kidney cell (tissue-associated) antigen molecule was assessed by the mAb I1F6 that recognizes 72 and 150 kDa tubular cell protein(s) (KT1). The MDCK cell line constitutively expressed low levels of MHC class II molecules and KT1. The steady-state level of the MHC class II mRNA transcription was virtually unaltered by treatment with IFN-gamma (400 units) for 48 hours; however, the MHC cell surface protein expression was enhanced. The KC and DK cell lines constitutively expressed KT1, but not MHC class II molecules; these cells required a minimum of 4000 units, and a 62-hour incubation with IFN-gamma was needed to upregulate both surface MHC class II molecules and the transcription of corresponding specific mRNA. In the MLKC reaction both the MDCK and DK cell lines, as well as fresh KC cells, could serve as lymphocyte activators. This could be amplified by exogenous IFN-gamma. The removal of APC from the responding T cell population did not reduce the IFN-gamma effect. This indicates that IFN-gamma treatment allows for the expression of all of the co-stimulating factors and/or adhesion molecules necessary for these cells to serve as (surrogate) APC (direct as opposed to indirect antigen presentation). The requirements for purified IFN-gamma to increase this amplification was greater in the MLKC reactions with kidney cells than in the MLC reactions. The mAbs anti-IFN-gamma and I1F6 differed in their ability to inhibit lymphocyte proliferation depending on the different cell types involved. The I1F6 inhibited the MDCK and DK cell-driven MLKC (in the absence of exogenous IFN-gamma).(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Surgery, University of Miami School of Medicine, FL 33101.
authors.forename: D
authors.initials: D
authors.lastname: Asthana
authors.forename: W C
authors.initials: WC
authors.lastname: Yang
authors.forename: L
authors.initials: L
authors.lastname: Fuller
authors.forename: K
authors.initials: K
authors.lastname: Zucker
authors.forename: P
authors.initials: P
authors.lastname: Lu
authors.forename: S
authors.initials: S
authors.lastname: Zheng
authors.forename: V
authors.initials: V
authors.lastname: Esquenazi
authors.forename: M
authors.initials: M
authors.lastname: Carreno
authors.forename: D
authors.initials: D
authors.lastname: Roth
authors.forename: G W
authors.initials: GW
authors.lastname: Burke
mesh_terms: D000293:Adolescent; D000328:Adult; D000954:Antigens, Surface / Q000096:biosynthesis* / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D003327:Coronary Disease / Q000209:etiology* / Q000276:immunology; D003331:Coronary Vessels / Q000276:immunology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D006680:HLA Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D006335:Heart Injuries / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D016027:Heart Transplantation / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007249:Inflammation; D008875:Middle Aged; D011184:Postoperative Period; D013312:Stress, Physiological / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014019:Tissue Donors
chemical_list: D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D006680:HLA Antigens