Analysis of polycystic kidney disease with two new microsatellite markers.
Two (CA) microsatellite polymorphisms have been studied in 209 subjects from Northern Italy, members of 27 Polycystic Kidney Disease families. Polymorphic alleles were analyzed by using a labelled PCR. Results obtained show that these markers highly improve the presymptomatic diagnosis of the disease in subjects at risk. In addition, our findings suggest that a region with a high recombination frequency should exist between PKD1 and SM7.
Bozza
A
A
Dipartimento di Biochimica e Biologia Molecolare, Universitá degli Studi di Ferrara.
Stefani
S
S
De Paoli Vitali
E
E
Perini
L
L
del Senno
L
L
D001483:Base Sequence; D002885:Chromosomes, Human, Pair 16; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D004276:DNA, Satellite; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D010375:Pedigree; D016891:Polycystic Kidney, Autosomal Dominant / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers; D004247:DNA