Effects of amiloride derivatives on the thermosensitivity of an adriamycin-resistant strain of Ehrlich ascites tumor cells.
We investigated the effects of amiloride and its derivative MH-12-43 on the thermosensitivity of adriamycin-resistant cells. Effects of hyperthermia at 42 degrees C were enhanced by 0.5 mM amiloride and 0.05 mM MH-12-43, which resulted in no decrease in survival rate by the treatment at 37 degrees C for 120 min. Therefore, the combination of hyperthermia and amiloride derivatives was effective in the adriamycin-resistant cells as well as wild type cells. Further, since the survival rates by the treatment with 0.05 mM MH-12-43 were markedly lower than those with 0.5 mM amiloride from hyperthermia at 42 degrees C, MH-12-43 might be more effective than amiloride.
Asaumi
J
J
Department of Radiology, Okayama University Medical School, Japan.
Kawasaki
S
S
Nishikawa
K
K
Kuroda
M
M
Hiraki
Y
Y
D000584:Amiloride / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology*; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D006358:Hot Temperature; D014407:Tumor Cells, Cultured
D000584:Amiloride; D004317:Doxorubicin; C039614:ethylisopropylamiloride