The chondroitin sulfate proteoglycan NG2 is a tumour-specific antigen on the chemically induced rat chondrosarcoma HSN.
N-terminal sequences of 19 and 11 amino acids obtained from 2 different tryptic fragments of the tumour-specific antigen on the chemically induced rat chondrosarcoma HSN show a 100% homology with the rat chondroitin sulfate proteoglycan NG2. Using a scheme of overlapping oligonucleotide primers we have cloned by PCR amplification the cDNA for the specific antigen of the HSN tumour that is immunogenic in immunocompetent CBH/Cbi rats and now report that its cDNA sequence is identical to that of NG2. The cDNA codes for a transmembrane protein of 2,325 amino acids with a large extracellular domain of 2,224 amino acids containing 2 cysteine-rich regions, a transmembrane domain (25 amino acids) and a short cytoplasmic tail (76 amino acids). The tumour-specific determinant was found to lie between amino acid residues 556 and 992 on the core glycoprotein.
Léger
O
O
Section of Immunology, Institute of Cancer Research, Sutton, Surrey, UK.
Johnson-Léger
C
C
Jackson
E
E
Coles
B
B
Dean
C
C
D000595:Amino Acid Sequence; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D001483:Base Sequence; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics*; D002813:Chondrosarcoma / Q000139:chemically induced / Q000276:immunology*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains
D015295:Antigens, Tumor-Associated, Carbohydrate; D011508:Chondroitin Sulfate Proteoglycans; D017931:DNA Primers; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D010446:Peptide Fragments