title: Human IL-10 reduces the number of IL-4-induced IgE B cells in cultures of atopic mononuclear cells.
abstract: We investigated the effect of recombinant human IL-10 on IL-4- and antigen-driven human peripheral blood mononuclear cell cultures derived from atopic donors. These cultures were phenotyped for the percentage of B cell (CD20 HLA-DR) population by flow cytometry and for intracellular IgE using epifluorescence microscopy. The addition of IL-4 (100 U/ml) to these cultures resulted in an increase in the percentage of IgE B cells. However, the percentage of IgE B cells in cultures coincubated with IL-10 (2 or 20 ng/ml) and IL-4 was reduced to the level of medium control. Peripheral-blood mononuclear (PBMN) cultures driven with dust mite allergen demonstrated a significant increase in cellular proliferation, as measured by 3[H] thymidine uptake, and in the percentage of IgE B cells. The coaddition of IL-10 (2 or 20 ng/ml) to these cultures significantly inhibited both proliferation and the mean percentage of IgE B cells. The inhibitory effect of IL-10 on IgE B cell percentages in both the IL-4- and the allergen-driven cultures, and on allergen-driven proliferation, was dependent upon the presence of monocytes. Interestingly, the inhibitory effect of IL-10 on allergen-driven proliferation in the atopic PBMN cultures was reversible by the coaddition of exogenous IFN gamma (1 ng/ml) and IL-2 (2 U/ml). The addition of IL-2 (2 U/ml) partially reversed IL-10-inhibited allergen-driven proliferation while alone IFN gamma had no effect (1 ng/ml). In fact, the addition of IFN gamma (1 ng/ml) in the absence of either IL-10 or IL-2 (2 U/ml) partially inhibited allergen-driven proliferation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Immunology, Schering-Plough Research Institute, Kenilworth, N.J. 07033.
authors.forename: L A
authors.initials: LA
authors.lastname: Bober
authors.forename: M J
authors.initials: MJ
authors.lastname: Grace
authors.forename: C
authors.initials: C
authors.lastname: Pugliese-Sivo
authors.forename: T A
authors.initials: TA
authors.lastname: Waters
authors.forename: L M
authors.initials: LM
authors.lastname: Sullivan
authors.forename: S K
authors.initials: SK
authors.lastname: Narula
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D002452:Cell Count / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000473:pathology*; D007073:Immunoglobulin E / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D011994:Recombinant Proteins; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E