title: High performance liquid chromatographic determination of azelastine and desmethylazelastine in guinea pig plasma and lung tissue.
abstract: Analytical methods have been developed for the simultaneous quantitation of azelastine (AZ) and desmethylazelastine (DAZ) in guinea pig plasma and lung tissue. The methods require a 1.00 mL plasma sample and a minimum 0.100 g lung sample. Both methods employ liquid/liquid organic extraction and back-extraction into dilute acid. Quantitation is performed by high performance liquid chromatography on a 2 x 250 mm 5 microns Hypersil CPS column using fluorescence detection. The linear quantitative ranges for AZ.HCl and DAZ.HBr in plasma are 0.156-160 ng/mL and 0.313-160 ng/mL, respectively. The linear quantitative ranges for AZ.HCl and DAZ.HBr in lung tissue are 0.039-20 micrograms/g for both.
authors.affiliation: Department of Biochemistry, Wallace Laboratories, Division of Carter-Wallace, Inc., Cranbury, NJ 08512.
authors.forename: C N
authors.initials: CN
authors.lastname: Langevin
authors.forename: J
authors.initials: J
authors.lastname: Pivonka
authors.forename: J K
authors.initials: JK
authors.lastname: Wichmann
authors.forename: N
authors.initials: N
authors.lastname: Kucharczyk
authors.forename: R D
authors.initials: RD
authors.lastname: Sofia
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D006634:Histamine H1 Antagonists / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D007202:Indicators and Reagents; D008168:Lung / Q000737:chemistry*; D010793:Phthalazines / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence
chemical_list: D006634:Histamine H1 Antagonists; D007202:Indicators and Reagents; D010793:Phthalazines; C054114:desmethylazelastine; C020976:azelastine