Interleukin-1 beta gene expression in experimental glomerulonephritis in the rat: an in-situ hybridization study.
To investigate the role of interleukin-1 beta (IL-1 beta) in glomerulonephritis we studied the presence of IL-1 beta by in-situ hybridization in in-situ immune complex glomerulonephritis in the rat. Glomerulonephritis was induced in preimmunized rats by unilateral renal perfusion with cationized human IgG. In-situ hybridization was performed on frozen sections with a battery of four 30mer oligonucleotide DNA probes 3' end labelled with 35S-dATP. IL-1 beta mRNA was detectable in nephritic glomeruli at 6 and 24 hours after induction of glomerulonephritis. Signal was maximal at 48 hours and markedly reduced by 4 days. The peak of IL-1 beta transcription coincided with the major monocyte influx into glomeruli consistent with a role for IL-1 beta as a mediator of glomerular hypercellularity.
Cook
H T
HT
Department of Histopathology, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, UK.
Avey
C
C
Taylor
G M
GM
D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D005921:Glomerulonephritis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D007105:Immune Complex Diseases / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000235:genetics*; D007678:Kidney Glomerulus / Q000276:immunology* / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew
D007375:Interleukin-1; D015345:Oligonucleotide Probes; D012333:RNA, Messenger