The expression of the bstVIM gene from Bacillus stearothermophilus V is restricted to vegetative cell growth.
The activity of BstVI DNA methyltransferase was monitored during the sporulative cycle of Bacillus stearothermophilus V. Significant methylase activity was found only in bacteria growing vegetatively. This was confirmed by Northern hybridization, which indicated that the bstVIM gene was not transcribed in cells undergoing sporulation. Supporting evidence came from experiments which demonstrated that the RNA polymerase holoenzyme from these cells did not recognize the promoter elements upstream of the bstVIM gene.
González
E
E
Laboratorio de Biología Molecular, Universidad de Talca, Chile.
Padilla
C
C
Saavedra
C
C
Vásquez
C
C
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis / Q000235:genetics; D004790:Enzyme Induction; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000096:biosynthesis / Q000235:genetics*; D013171:Spores, Bacterial
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C057988:DNA modification methylase BstVI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D012321:DNA-Directed RNA Polymerases; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific