Insulin-responsive regions of the rat fatty acid synthase gene promoter.
Here we show that insulin may play a role in the diet-induced regulation of the rat fatty acid synthase (FAS; EC 2.3.1.85). Transient transfection of human and rat hepatoma cell lines with successively deleted FAS/CAT promoter fusion plasmids was used to determine the effect of insulin on FAS promoter activity. Our results indicate the existence of cis-acting insulin-responsive elements in the FAS promoter; the position of one of these is coincident with the position of a previously determined diet-induced DNAse I hypersensitive site (HSi-1) at approximately -500 bp relative to the transcription start site of FAS mRNA.
Wolf
S S
SS
Institute of Food Research, Genetics and Microbiology Department, Colney, Norwich, United Kingdom.
Hofer
G
G
Beck
K F
KF
Roder
K
K
Schweizer
M
M
D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D003850:Deoxyribonuclease I / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D008113:Liver Neoplasms; D008114:Liver Neoplasms, Experimental; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D007328:Insulin; D012333:RNA, Messenger; D064429:Fatty Acid Synthases; D003850:Deoxyribonuclease I