Thiamine-repressible genes in Schizosaccharomyces pombe are regulated by a Cys6 zinc-finger motif-containing protein.
Our previous genetic data indicate that the product of the Schizosaccharomyces pombe thi1 gene acts as an activator of several thiamine-repressible genes which are involved in the control of thiamine metabolism [Schweingruber et al., Genetics 130 (1992) 445-449; Zurlinden and Schweingruber, Gene 117 (1992) 141-143]. In this communication, we report the cloning and sequencing of thi1 and show that it carries an open reading frame which translates into a 775-amino-acid protein with the characteristics of a Cys6 zinc-finger-motif-containing transcription factor, as typified by Saccharomyces cerevisae GAL4. We, therefore, suggest that the thi1-encoded protein binds to upstream activator sequences of thiamine-repressible genes.
Fankhauser
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Institute of General Microbiology, University of Berne, Switzerland.
Schweingruber
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ME
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013831:Thiamine / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C086956:thi1 protein, S pombe; D013831:Thiamine