Expression and characterization of a parasite-specific antigen on macrophages after infection with Leishmania donovani.
A rabbit polyclonal antibody to crude soluble antigen of Leishmania donovani promastigotes recognized a determinant expressed on the surface membrane of mouse peritoneal macrophages and human monocyte derived macrophages infected in vitro. The determinant was recognized on infected macrophage surface only when F(ab')2 fragments of anti-leishmanial antiserum was employed in immunofluorescence. F(ab')2 fragments of human patient sera also could recognize the determinant. The expression of this antigen was not stage-specific for the parasite. Immunochemical analyses revealed this antigen to be of 51 kDa protein. Specific leaching of membrane proteins by trypsin showed three bands of expressed antigens of 26, 11 and 10 kDa, which in all likelihood might be arising from the 51 kDa antigen. The antigen was not expressed until 12 h of post infection, reached a maximum level at 24 h and thereafter attained a steady state level as studied upto 96 h of post infection. This type of antigen might have a great potential in immunodiagnostics and site-specific drug targeting.
Basu
N
N
Department of Molecular Cell Biology, Indian Institute of Chemical Biology, Calcutta.
Kole
L
L
Ghosh
A
A
Das
P K
PK
D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007700:Kinetics; D007893:Leishmania donovani / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000469:parasitology*; D051379:Mice; D008970:Molecular Weight
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007140:Immunoglobulin Fab Fragments