[A PCR-system of analyzing polymorphic markers in gamma-A and gamma-G globin genes and gene for human blood coagulation factor IX with an internal control of the completeness of restriction hydrolysis].
New systems are proposed for the PCR analysis of HindIII polymorphic sites in the gamma A and gamma G globin genes and of TaqI polymorphic site in the human factor IX gene of blood population. DNA fragments amplified according to the systems described contain constant restriction site of the appropriate endonuclease, in addition to the polymorphic one, which significantly improves the reliability of the RELP analysis. The systems proposed are highly specific and may be used for DNA diagnosis of beta-thalassemia and haemophilia B.
Luk'ianenko
A V
AV
Surin
V L
VL
Voskoboeva
E Iu
EIu
Tagiev
A F
AF
Krutov
A A
AA
Solov'ev
G Ia
GIa
D001483:Base Sequence; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D005164:Factor IX / Q000235:genetics*; D005819:Genetic Markers; D005914:Globins / Q000235:genetics*; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D005819:Genetic Markers; D005164:Factor IX; D005914:Globins; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases
PTsR-sistemy analiza polimorfnykh markerov v gamma-A i gamma-G globinovykh genakh i gene faktora koaguliatsii krovi IX cheloveka s vnutrennim kontrolem polnoty restriktsionnogo gidroliza.