title: Fluid secretion by isolated tick salivary glands dependent on an intact cytoskeleton.
abstract: Isolated salivary glands from female Dermacentor variabilis (Say) were pre-treated with either cytochalasin D or nocodazol, followed by stimulation with dopamine. Glands pre-treated with 1 microM-cytochalasin D did not secrete fluid; pre-treatment with 1 nM-cytochalasin D did not significantly reduce fluid secretion. Glands pre-treated with 1 microM-nocodazol had a significant reduction in fluid secretion (P = 0.003); 1 nM-nocodazol did not significantly affect fluid secretion. Ligation of the main salivary duct and pre-treatment with 1 microM-cytochalasin D significantly increased gland weight compared to the dopamine stimulated controls (P = 0.0412). Cytochalasin D-treated type III acini had a significantly larger mean diameter compared to the dopamine control (P = 0.0047). Glands treated with 1 mM-verapamil plus 10 microM-dopamine exhibited a significant decrease in fluid secretion (P = 0.017), and when ligated, had a significantly decreased weight compared to the controls (P = 0.0028).
authors.affiliation: Department of Biology, Memphis State University, TN 38152.
authors.forename: W J
authors.initials: WJ
authors.lastname: Lamoreaux
authors.forename: G R
authors.initials: GR
authors.lastname: Needham
authors.forename: L B
authors.initials: LB
authors.lastname: Coons
mesh_terms: D000818:Animals; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology*; D003870:Dermacentor / Q000378:metabolism*; D004298:Dopamine / Q000494:pharmacology; D005260:Female; D008297:Male; D015739:Nocodazole / Q000494:pharmacology; D012469:Salivary Glands / Q000187:drug effects / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology
chemical_list: D015638:Cytochalasin D; D014700:Verapamil; D015739:Nocodazole; D004298:Dopamine