title: Biocompatibility and tissue regenerating capacity of crosslinked dermal sheep collagen.
abstract: The biocompatibility and tissue regenerating capacity of four crosslinked dermal sheep collagens (DSC) was studied. In vitro, the four DSC versions were found to be noncytotoxic or very low in cytotoxicity. After subcutaneous implantation in rats, hexamethylenediisocyanate-crosslinked DSC (HDSC) seldom induced an increased infiltration of neutrophils or macrophages, as compared with normal wound healing; whereas new formation of collagen was observed. DSC crosslinked with glutaraldehyde (GDSC) followed by reaction with NaBH4 shortly after implantation showed an increased infiltration of neutrophils with a deviant morphology. Furthermore, a high incidence of calcification was observed, which may explain the minor ingrowth of giant cells and fibroblasts, and the poor formation of new rat collagen. Acyl azide-crosslinked DSC (AaDSC) first induced an increased infiltration of macrophages, and then of giant cells, both with high lipid formation. AaDSC degraded at least twice as slowly as HDSC and GDSC, finally leaving a matrix of newly formed rat collagen. Samples crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide (ENDSC) induced the same mild cellular reaction as HDSC; whereas, similar to AaDSC, the degradation rate was slow and an optimal rat collagen matrix was formed. Of the crosslinked DSC samples, ENDSC seems most promising for tissue regeneration.
authors.affiliation: Department of Histology and Cell Biology, University of Groningen, The Netherlands.
authors.forename: P B
authors.initials: PB
authors.lastname: van Wachem
authors.forename: M J
authors.initials: MJ
authors.lastname: van Luyn
authors.forename: L H
authors.initials: LH
authors.lastname: Olde Damink
authors.forename: P J
authors.initials: PJ
authors.lastname: Dijkstra
authors.forename: J
authors.initials: J
authors.lastname: Feijen
authors.forename: P
authors.initials: P
authors.lastname: Nieuwenhuis
mesh_terms: D000818:Animals; D001386:Azides / Q000494:pharmacology; D001672:Biocompatible Materials / Q000494:pharmacology* / Q000633:toxicity; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000187:drug effects / Q000494:pharmacology* / Q000633:toxicity; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D003485:Cyanates / Q000494:pharmacology; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology; D005347:Fibroblasts; D005549:Foreign-Body Reaction / Q000209:etiology / Q000473:pathology; D005976:Glutaral / Q000494:pharmacology; D017953:Isocyanates; D008297:Male; D008422:Materials Testing; D019736:Prostheses and Implants; D051381:Rats; D012756:Sheep; D013388:Succinimides / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001386:Azides; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D003485:Cyanates; D017953:Isocyanates; D013388:Succinimides; C015262:1,6-hexamethylene diisocyanate; D003094:Collagen; C001426:N-hydroxysuccinimide; D005022:Ethyldimethylaminopropyl Carbodiimide; D005976:Glutaral