title: Induction of metallothionein mRNA in the blue crab (Callinectes sapidus) after treatment with cadmium.
abstract: 1. Dietary treatment of blue crabs with cadmium led to an increase of cadmium metallothionein in the hepatopancreas. 2. Utilizing a unique purification procedure, poly A+ RNA was isolated from the hepatopancreas of control and cadmium-treated blue crabs with 260/280 nm ratios of 2.0 or better. 3. Poly A+ RNA was hybridized with a series of oligonucleotide probes derived from a portion of the amino acid sequence of lobster metallothionein. A single band of approx. 300 base pairs was present in control and cadmium-treated RNA from the hepatopancreas of blue crab. 4. A four-fold induction of the band occurred following cadmium exposure.
authors.affiliation: Division of Toxicology, University of Arkansas for Medical Sciences, Little Rock 72205.
authors.forename: D
authors.initials: D
authors.lastname: Schlenk
authors.forename: M
authors.initials: M
authors.lastname: Brouwer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000187:drug effects / Q000235:genetics*; D002104:Cadmium / Q000494:pharmacology*; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000187:drug effects
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger; C004203:cadmium-binding protein; D002104:Cadmium; D008668:Metallothionein