title: Effect of protein kinase C activators on the uptake of GABA by rat brain synaptosomes.
abstract: Synaptosomes were prepared from rat brain by a discontinuous Ficoll gradient method and used for studying the uptake of labelled GABA. Two GABA uptake components were evidenced, a high (Km = 3.13 microM) and a low (Km = 92.4 microM) affinity one. Preincubation of synaptosomes with two different activators of protein kinase C, phorbol 12, 13-diacetate (PDAc) and oleyl-acetyl glycerol (OAG), resulted in a change of GABA uptake. In particular, the low affinity component increased its Vmax by 58-74%, with no change in the Km. No statistically significant modification was detected for the high affinity component.
authors.affiliation: Centro di Neurofisiologia, C.N.R., Genova, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cupello
authors.forename: B
authors.initials: B
authors.lastname: Gasparetto
authors.forename: P
authors.initials: P
authors.lastname: Mainardi
authors.forename: L
authors.initials: L
authors.lastname: Vignolo
authors.forename: M
authors.initials: M
authors.lastname: Robello
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D046508:Culture Techniques; D004075:Diglycerides / Q000494:pharmacology*; D008297:Male; D010703:Phorbol Esters / Q000494:pharmacology*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013574:Synaptosomes / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D010703:Phorbol Esters; C008874:phorbol-12,13-diacetate; D005680:gamma-Aminobutyric Acid; C038237:1-oleoyl-2-acetylglycerol; D011493:Protein Kinase C