Thiolase mRNA translated in vitro yields a peptide with a putative N-terminal presequence.
Thiolase is part of the fatty acid oxidation machinery which in plants is located within glyoxysomes or peroxisomes. In cucumber cotyledons, proteolytic modification of thiolase takes place during the transfer of the cytosolic precursor into glyoxysomes prior to the intraorganellar assembly of the mature enzyme. This was shown by size comparison of the in vitro synthesized precursor and the 45 kDa subunit of the homodimeric glyoxysomal form. We isolated a full-length cDNA clone encoding the 48,539 Da precursor of thiolase. This plant protein displayed 40% and 47% identity with the precursor of fungal peroxisomal thiolase and human peroxisomal thiolase, respectively. Compared to bacterial thiolases, the precursor of the plant enzyme was distinguished by an N-terminal extension of 34 amino acid residues. This putative targeting sequence of cucumber thiolase shows similarities with the cleavable presequences of rat peroxisomal thiolase and plant peroxisomal malate dehydrogenase.
Preisig-Müller
R
R
University of Marburg, Department of Chemistry, FRG.
Kindl
H
H
D000101:Acetyl-CoA C-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001693:Biological Transport, Active; D002451:Cell Compartmentation; D002474:Cell-Free System; D003001:Cloning, Molecular; D017343:Genes, Plant / Q000235:genetics; D014644:Genetic Variation; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D010945:Plants, Edible / Q000201:enzymology / Q000235:genetics; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D021382:Protein Sorting Signals / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D011498:Protein Precursors; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D000101:Acetyl-CoA C-Acetyltransferase