Autoimmune myocarditis induced in mice by cardiac C-protein. Cloning of complementary DNA encoding murine cardiac C-protein and partial characterization of the antigenic peptides.
Autoimmune myocarditis is considered to play a major role in the pathogenesis of dilated cardiomyopathy. A new autoimmune myocarditis model was attained by repeated immunization using murine cardiac C-protein with the immunological adjuvant, Klebsiella pneumoniae O3 lipopolysaccharide. For further analysis of a pathological epitope, the cDNA encoding C-protein was isolated; a fusion protein encoded by part of this cDNA induced myocarditis in SMA mice as well as in three other strains: DBA/1J (H-2q), O20/A (H-2pz1), and SJL (H-2s). The nucleotide sequence and its deduced amino acid analysis revealed that this protein had immunoglobulin-like and fibronectin-like repeats. This study provides a new animal model of autoimmune myocarditis which may shed light on the pathogenesis of dilated cardiomyopathy.
Kasahara
H
H
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Itoh
M
M
Sugiyama
T
T
Kido
N
N
Hayashi
H
H
Saito
H
H
Tsukita
S
S
Kato
N
N
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000139:chemically induced / Q000473:pathology*; D002311:Cardiomyopathy, Dilated / Q000097:blood / Q000276:immunology*; D002352:Carrier Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D006321:Heart / Q000187:drug effects; D006801:Humans; D007249:Inflammation; D007711:Klebsiella pneumoniae; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D008875:Middle Aged; D009124:Muscle Proteins / Q000096:biosynthesis / Q000633:toxicity*; D009205:Myocarditis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology*; D009206:Myocardium / Q000473:pathology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000633:toxicity; D012016:Reference Values; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D000099163:Myosin Binding Protein C
D001323:Autoantibodies; D002352:Carrier Proteins; D018076:DNA, Complementary; D008070:Lipopolysaccharides; D009124:Muscle Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D000099163:Myosin Binding Protein C