Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion.
The structure of the repeat region and chain caps of the N-linked keratan sulphate chains attached to bovine tracheal cartilage fibromodulin has been examined. The chains were fragmented by keratanase digestion, the resultant oligosaccharides isolated by strong anion-exchange chromatography, and their structures determined using high-field 1H-n.m.r. spectroscopy. The chains were found to possess the following general structure: [formula: see text] All of the capping oligosaccharides isolated terminate with alpha(2-3)-linked N-acetylneuraminic acid. No alpha(2-6)-linked N-acetylneuraminic acid chain terminators, nor any fucose, alpha (1-3)-linked to N-acetylglucosamine along the repeat region, were detected. This work demonstrates that the structure of the repeat region and chain caps of N-linked keratan sulphate attached to fibromodulin isolated from bovine tracheal cartilage is identical with that of O-linked keratan sulphate chains attached to aggrecan derived from non-articular cartilage.
Lauder
R M
RM
Division of Biological Sciences, University of Lancaster, Bailrigg, U.K.
Huckerby
T N
TN
Nieduszynski
I A
IA
D000818:Animals; D002240:Carbohydrate Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D002356:Cartilage / Q000737:chemistry*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D016326:Extracellular Matrix Proteins; D000071235:Fibromodulin; D006026:Glycoside Hydrolases; D007632:Keratan Sulfate / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D011509:Proteoglycans; D014132:Trachea; D001616:beta-Galactosidase / Q000378:metabolism
D002352:Carrier Proteins; D016326:Extracellular Matrix Proteins; D009844:Oligosaccharides; D011509:Proteoglycans; D000071235:Fibromodulin; D007632:Keratan Sulfate; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase