Transposition pattern of a modified Ds element in tomato.
Several aspects of transposition of an in vitro modified Ds element are described. This Ds element, designated Ds-r, is equipped with bacterial plasmid sequences and can, therefore, be rescued from the plant genome. Our results indicate that the Ds-r element has a 'late' timing of transposition from T-DNAs. This feature of the element might be advantageous for tagging experiments because it leads to independently transposed germinally transmitted elements. Furthermore, it is shown that Ds-r transposition generates clusters of insertions, indicating that 'genes to be tagged' should be located in genomic regions covered by insertions.
Rommens
C M
CM
Department of Genetics, Free University, Amsterdam, Netherlands.
Munyikwa
T R
TR
Overduin
B
B
Nijkamp
H J
HJ
Hille
J
J
D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C036483:T-DNA; D004247:DNA