Fast-protein liquid chromatography system as a tool for liposome preparation by the extrusion procedure.
Employment of fast-protein liquid chromatography (FPLC) system and a high-pressure filtration cell is described for the production of liposomes by the rapid extrusion technique. The system allows processing of up to 50 ml liposomal suspension in one batch at a defined temperature and applied pressure of up to 4.5 MPa. This relatively simple technique is applicable in any laboratory equipped with FPLC or HPLC systems.
Turánek
J
J
Department of Immunology, Veterinary Research Institute, Brno, Czech Republic.
D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D005374:Filtration / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000138:chemical synthesis* / Q000737:chemistry; D010743:Phospholipids / Q000138:chemical synthesis / Q000737:chemistry; D011312:Pressure; D011506:Proteins
D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D011506:Proteins