title: Immunohistochemical and functional studies on rat high endothelial venules using monoclonal antibodies.
abstract: The distribution of high endothelial venules (HEVs) in various rat organs has been investigated immunohistochemically using two monoclonal antibodies (MoAbs), REC16-11 and REC4-1. The staining patterns observed with REC16-11 and REC4-1 were compared with those obtained with the MoAb anti-rat ICAM-1, which is a cell adhesion molecule in HEVs. REC16-11 reacted not only with HEVs but also with all vascular endothelial cells (ECs) in the tissues examined. Electronmicroscopy showed that REC16-11 and REC4-1 reacted with the cell surface antigens of ECs and immunoblot analysis of rat splenic stromal preparations showed that REC4-1 stained 42 kd and 60 kd bands. REC4-1 inhibited the binding of lymphocyte to HEVs in the mucosa-associated lymphoid tissues (MALT), but had no effects on lymphocyte binding to HEVs in peripheral (non-mucosal) lymph nodes. These findings suggested that the MoAb REC4-1 recognized the associated antigen of the lymphocyte-HEV recognition system in MALT.
authors.affiliation: Department of Pathology, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohbayashi
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: A
authors.initials: A
authors.lastname: Nakayama
authors.forename: K
authors.initials: K
authors.lastname: Kasai
authors.forename: J
authors.initials: J
authors.lastname: Asai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology; D002645:Chickens; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1; D008214:Lymphocytes / Q000378:metabolism; D008221:Lymphoid Tissue / Q000166:cytology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009092:Mucous Membrane / Q000166:cytology; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D013154:Spleen / Q000737:chemistry; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1