title: Amphibian Pleurodeles waltl fibronectin: cDNA cloning and developmental expression of spliced variants.
abstract: Partial cDNA clones encoding approximately the carboxy terminal half of Pleurodeles fibronectin (FN) were isolated. They account for 4.7 Kbp of the 3' region of the FN mRNA. The cDNA nucleotide sequence comprises all three alternatively spliced segments designated EIIIA, EIIIB and V-segment, respectively. All three segments are included in FN mRNA synthesized during early embryogenesis whereas, from the tailbud stage onward the V-region was partially excluded. The isolation of Pleurodeles cDNA clones including the three different spliced EIIIA, EIIIB and V segment raises new possibilities for the study of the precise role of specific regions of FN in early amphibian development.
authors.affiliation: Laboratoire de Biologie Expérimentale, URA CNRS 1135, Université P. et M. Curie, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Clavilier
authors.forename: J F
authors.initials: JF
authors.lastname: Riou
authors.forename: D L
authors.initials: DL
authors.lastname: Shi
authors.forename: D W
authors.initials: DW
authors.lastname: DeSimone
authors.forename: J C
authors.initials: JC
authors.lastname: Boucaut
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005314:Embryonic and Fetal Development / Q000235:genetics; D005353:Fibronectins / Q000235:genetics*; D015870:Gene Expression; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010999:Pleurodeles / Q000196:embryology / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D018076:DNA, Complementary; D005353:Fibronectins