cAMP, ethanol, and CO2 production with the addition of D-glucose anomer to starved yeast cells.
cAMP, ethanol, and CO2 production in starved yeast cells after the addition of D-glucose anomer was measured and compared over a wide range of anomer concentrations. At 1.0 g/l or higher concentrations, the addition of beta-D-glucose resulted in a higher cAMP peak. beta-D-glucose was more rapidly metabolized to ethanol and CO2 than alpha-D-glucose, although there was no notable difference in the uptake rates of the two anomers. At 0.4 g/l D-glucose anomer, the differences in cAMP and ethanol production rates for the two anomers were not significant. At 0.2 g/l D-glucose anomer or lower concentrations, ethanol production with alpha-D-glucose was higher than that with beta-D-glucose. The uptake rate of alpha-D-glucose was higher than that of beta-D-glucose at this low concentration.
Han
K
K
Department of Chemical and Biochemical Engineering, University of California, Irvine 92717.
Hong
J
J
Lim
H C
HC
D002245:Carbon Dioxide / Q000032:analysis / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007536:Isomerism; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013997:Time Factors
D002245:Carbon Dioxide; D000431:Ethanol; D000242:Cyclic AMP; D005947:Glucose