Evidence of pestivirus RNA in human virus vaccines.
We examined live virus vaccines against measles, mumps, and rubella for the presence of pestivirus RNA or of pestiviruses by reverse transcription PCR. Pestivirus RNA was detected in two measles-mumps-rubella combined vaccines and in two monovalent vaccines against mumps and rubella. Nucleotide sequence analysis of the PCR products indicated that a modified live vaccine strain used for immunization of cattle against bovine viral diarrhea is not responsible for the contamination of the vaccines.
Harasawa
R
R
Animal Center for Biomedical Research, Faculty of Medicine, University of Tokyo, Japan.
Tomiyama
T
T
D001483:Base Sequence; D001908:Diarrhea Viruses, Bovine Viral / Q000145:classification / Q000235:genetics; D004338:Drug Combinations; D004340:Drug Contamination; D008458:Measles Vaccine / Q000737:chemistry / Q000592:standards; D022542:Measles-Mumps-Rubella Vaccine; D008969:Molecular Sequence Data; D009108:Mumps Vaccine / Q000737:chemistry / Q000592:standards; D010576:Pestivirus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification*; D012411:Rubella Vaccine / Q000737:chemistry / Q000592:standards; D012680:Sensitivity and Specificity; D014613:Vaccines, Attenuated
D004338:Drug Combinations; D008458:Measles Vaccine; D022542:Measles-Mumps-Rubella Vaccine; D009108:Mumps Vaccine; D012367:RNA, Viral; D012411:Rubella Vaccine; D014613:Vaccines, Attenuated