Two-dimensional crystallization of proteins on planar lipid films and structure determination by electron crystallography.
Electron crystallography constitutes a powerful new method for determining the structure of biological macromolecules. This method is best adapted to the study of ordered assemblies of macromolecules, and principally to two-dimensional (2-D) crystals of proteins. Obtaining protein 2-D crystals ordered at high resolution constitutes the major limiting step in the application of this approach. Considerable interest has been raised by the development of a rational method of 2-D crystallization based on the specific binding of proteins to planar lipid films. The applicability of this method is quasi-general in the case of soluble proteins. Its basic principles, together with examples taken from work in our group, are presented here.
Brisson
A
A
Laboratoire de Génétique Moléculaire des Eucaryotes, CNRS, Strasbourg, France.
Olofsson
A
A
Ringler
P
P
Schmutz
M
M
Stoylova
S
S
D003460:Crystallization; D003461:Crystallography / Q000379:methods*; D008024:Ligands; D008055:Lipids; D008854:Microscopy, Electron / Q000379:methods*; D008961:Models, Structural; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure*
D008024:Ligands; D008055:Lipids; D011506:Proteins