title: LH- and chorionic gonadotrophin-stimulated progesterone release in vitro by intact luteal tissue of the marmoset monkey (Callithrix jacchus).
abstract: The application of an in vitro microdialysis system (MDS) for studies on the gonadotrophic control of luteal progesterone secretion in the marmoset monkey is described. Luteal tissue collected from a total of six animals (9 +/- 1 days after ovulation) was perfused with Ringer solution (without and with lipoprotein, 0.6 microgram/ml). The tissue was exposed to repeated applications of human LH (hLH) and human chorionic gonadotrophin (hCG) (1, 10 and 100 IU/ml) each of 60 min duration. Perfusate was collected in 15-min fractions and assayed for progesterone content. Results showed that addition of lipoproteins to the Ringer solution had a marked effect of progesterone secretion in terms of maintaining stable baseline levels and improving reproducibility of gonadotrophin-induced responses. Progesterone secretion was significantly stimulated by both gonadotrophins at each dose tested. Maximal elevations were obtained with 10 IU/ml and there were no apparent differences in responses to hLH and hCG in terms of either magnitude or duration. This study indicates that MDS provides a useful in vitro approach for studying the gonadotrophic control of the corpus luteum in non-human primates. The results did not demonstrate disparate actions of hLH and hCG in their ability to stimulate luteal progesterone secretion.
authors.affiliation: Department of Reproductive Biology, German Primate Center, Göttingen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Einspanier
authors.forename: J K
authors.initials: JK
authors.lastname: Hodges
mesh_terms: D000818:Animals; D002144:Callithrix / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005260:Female; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology*; D017551:Microdialysis; D010477:Perfusion; D011374:Progesterone / Q000032:analysis / Q000378:metabolism*; D013268:Stimulation, Chemical
chemical_list: D006063:Chorionic Gonadotropin; D003470:Culture Media; D008074:Lipoproteins; D011374:Progesterone; D007986:Luteinizing Hormone