title: Application to cows and horses of Spotchem, a dry-chemistry blood analyzer for use in veterinary clinics.
abstract: The usefulness of a dry-chemistry blood analyzer, Spotchem SP-4410 (SP-4410) in a veterinary clinic for analysis of bovine and equine blood chemistry was studied. We quantitated total protein (TP), albumin (Alb), total bilirubin (T-Bil), blood urea nitrogen (BUN), total cholesterol (T-Cho), glucose (Glu), calcium (Ca), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), creatinine phosphokinase (CPK), and alkaline phosphatase (ALP) in bovine sera. Each sample was assayed with both the SP-4410 and an automated blood analyzer which served as a wet-chemistry reference system, and the data were analyzed with regression analysis. The correlation coefficient for AST was 0.997 being the highest for all the parameters, and all the correlation coefficients were 0.93 or higher. The coefficients of variation were lower than 5.0 except in the case of bovine T-Bil where it was 5,756. The ranges of normal reference values measured by SP-4410 were the same as those reported by other investigators in most cases, but those for GGT and CPK were slightly higher. The strongest interference was observed with hemoglobin. It seems that dry-chemical-analysis of blood serum using the SP-4410 is useful for analysis of bovine and equine blood.
authors.affiliation: Department of Veterinary Internal Medicine, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori Japan.
authors.forename: F
authors.initials: F
authors.lastname: Hoshi
authors.forename: M
authors.initials: M
authors.lastname: Satho
authors.forename: S
authors.initials: S
authors.lastname: Koyama
authors.forename: K
authors.initials: K
authors.lastname: Nakadaka
authors.forename: M
authors.initials: M
authors.lastname: Chiba
authors.forename: N
authors.initials: N
authors.lastname: Ikeda
authors.forename: R
authors.initials: R
authors.lastname: Hakamada
authors.forename: S
authors.initials: S
authors.lastname: Higuchi
authors.forename: S
authors.initials: S
authors.lastname: Kawamura
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000662:veterinary*; D002417:Cattle / Q000097:blood*; D005260:Female; D006736:Horses / Q000097:blood*; D012016:Reference Values