Effects of calcium chelators on intracellular calcium and excitotoxicity.
In an attempt to probe the relationship between excitotoxicity and increases in intracellular calcium ([Ca2+]i), BAPTA-AM and its analogs were applied to cultured hippocampal neurons. Chelation of [Ca2+]i depressed and prolonged transient responses to glutamate and did not effect elevation of [Ca2+]i by prolonged exposure. This explains the inability of the chelators to prevent glutamate-induced toxicity.
Dubinsky
J M
JM
Department of Physiology, University of Texas Health Science Center, San Antonio 78284-7756.
D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology*; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004759:Enterochromaffin Cells / Q000187:drug effects; D005971:Glutamates / Q000633:toxicity; D018698:Glutamic Acid; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D051381:Rats; D017470:Receptors, Glutamate / Q000187:drug effects
D002614:Chelating Agents; D005971:Glutamates; D017470:Receptors, Glutamate; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D018698:Glutamic Acid; D004533:Egtazic Acid; D002118:Calcium