title: Evidence for formation of somatic cell hybrids in a population of irradiated cells.
abstract: Experiments using two genetically marked lines of Djungarian hamster cells (DM-15 HPRT- and DH-TK-) and the technique of hybrid selection in selective HAT medium revealed viable colonies in a mixed culture irradiated with a dose of 5 Gy. The sublines grown from these colonies were examined. Chromosome analysis showed that about 45% of those cells were hybrids inheriting chromosome markers of both parent strains. Formation of radio-induced hybrids occurs as a function of time after irradiation, 6 days proving to be the optimal interval. It is postulated that radiation-induced cell fusion and formation of viable somatic cell hybrids may be essential for cell population survival in the course of tumour radiotherapy.
authors.affiliation: Cancer Research Center of RAMS, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Andrushkevich
authors.forename: G S
authors.initials: GS
authors.lastname: Kalendo
authors.lastname: Semenyak OJu
authors.forename: E S
authors.initials: ES
authors.lastname: Kakpakova
mesh_terms: D000818:Animals; D006224:Cricetinae; D005819:Genetic Markers; D006822:Hybrid Cells; D007621:Karyotyping; D017111:Phodopus; D011831:Radiation Genetics
chemical_list: D005819:Genetic Markers