title: The induction and repression of benzene and catechol oxidizing capacity of Pseudomonas putida ML2 studied in perturbed chemostat culture.
abstract: The oxidation of catechol, an intermediate in benzene catabolism, was studied using transient variations in dissolved oxygen tension (DOT) when a succinate limited steady state culture of Pseudomonas putida ML2 was perturbed with a pulse of another substrate. A model was developed and tested for the effect of fluctuations in oxidizing enzyme activity on DOT. It was found that the rate of induction of catechol oxidizing enzymes was independent of dilution rate up to a relative growth rate (mu/mumax) of 0.75. Only at higher dilution rates was catabolite repression observed.
authors.affiliation: Division of Life Sciences, King's College London, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: Mason
mesh_terms: D001554:Benzene / Q000378:metabolism*; D050548:Catechol 1,2-Dioxygenase; D002396:Catechols / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D049308:Dioxygenases; D004790:Enzyme Induction; D004794:Enzyme Repression; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D010105:Oxygenases / Q000096:biosynthesis*; D016958:Pseudomonas putida / Q000201:enzymology / Q000378:metabolism*
chemical_list: D002396:Catechols; D003470:Culture Media; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D001554:Benzene; D010100:Oxygen