title: Sequence of the pyr4 gene encoding orotidine-5'-phosphate decarboxylase from the biocontrol fungus Trichoderma harzianum.
abstract: The pyr4 gene, encoding orotidine-5'-phosphate decarboxylase (OMP decarboxylase) from the biocontrol fungus Trichoderma harzianum, has been isolated by hybridization, using a polymerase chain reaction (PCR)-derived fragment as a probe. The PCR primers corresponded to conserved regions of OMP decarboxylase-encoding genes from other filamentous fungi. A 2490-bp genomic fragment, which complemented a pyr4-auxotrophic T. reesei mutant, was sequenced. The gene showed high homology to pyr4 genes from other pyrenomycetes.
authors.affiliation: Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Austria.
authors.forename: E J
authors.initials: EJ
authors.lastname: Heidenreich
authors.forename: C P
authors.initials: CP
authors.lastname: Kubicek
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D014242:Trichoderma / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D009964:Orotidine-5'-Phosphate Decarboxylase