Immunization of rabbits with enterotoxigenic E. coli colonization factor antigen (CFA/I) encapsulated in biodegradable microspheres of poly (lactide-co-glycolide).
We have searched for an effective oral delivery system for a purified enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin, CFA/I, which elicits anti-colonization immunity. Purified CFA/I antigen encapsulated in biodegradable polymer microspheres of poly (DL-lactide-co-glycolide) (PLG) induced a vigorous, systemic CFA/I IgG antibody response in rabbits immunized once via intragastric tube; oral, unencapsulated CFA/I induced little or no circulating antibody. CFA/I-specific, S-IgA coproantibody was detected in one of three rabbits fed with the CFA/I-PLG microsphere vaccine. We conclude that PLG microspheres protected CFA/I from degradation in the stomach and effectively delivered the antigen for processing by the host's immune system.
Edelman
R
R
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201.
Russell
R G
RG
Losonsky
G
G
Tall
B D
BD
Tacket
C O
CO
Levine
M M
MM
Lewis
D H
DH
D018830:Adhesins, Escherichia coli; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000276:immunology*; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D001673:Biodegradation, Environmental; D004339:Drug Compounding; D004926:Escherichia coli / Q000276:immunology*; D022361:Escherichia coli Vaccines; D005243:Feces; D036781:Fimbriae Proteins; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis* / Q000276:immunology; D007273:Injections, Intramuscular; D008863:Microspheres; D011098:Polyglactin 910 / Q000493:pharmacokinetics; D011817:Rabbits
D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D022361:Escherichia coli Vaccines; D007071:Immunoglobulin A, Secretory; C022924:colonization factor antigens; D036781:Fimbriae Proteins; D011098:Polyglactin 910