Determination of fumonisins in milk.
Fumonisin B1 (FB1) and fumonisin B2 (FB2) were determined in milk by liquid chromatography (LC) following immunoaffinity column cleanup. Recoveries from milk spiked with 5-50 ng each fumonisin/ml averaged 79-109%. The aminopentol hydrolysis product of FB1 (AP1) was determined by LC after cleanup on a C18 solid phase phase extraction column; mean recoveries were 69-83% at spiking levels of 50-100 ng AP1/ml milk. Detection limits were of the order 3-7 ng/ml for FB1 and FB2, and 20-25 ng/ml for AP1. A stability study showed no losses of FB1 and FB2 in milk under conditions of freezing, refrigeration and boiling. A transmission study using four cows dosed with pure FB1 either orally (1.0 and 5.0 mg FB1/kg b.w.) or by i.v. injection (0.05 and 0.20 mg FB1/kg b.w.) showed no detectable residues of FB1 or AP1 in the milk, with or without hydrolytic treatment with beta-glucuronidase/sulfatase to liberate any conjugates.
Scott
P M
PM
Food Research Division, Bureau of Chemical Safety, Food Directorate, Ottawa, Ontario, Canada.
Delgado
T
T
Prelusky
D B
DB
Trenholm
H L
HL
Miller
J D
JD
D000284:Administration, Oral; D000818:Animals; D002274:Carcinogens, Environmental / Q000032:analysis*; D002417:Cattle; D002853:Chromatography, Liquid; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D037341:Fumonisins; D006361:Heating; D006868:Hydrolysis; D007275:Injections, Intravenous; D008892:Milk / Q000737:chemistry*; D009183:Mycotoxins / Q000008:administration & dosage / Q000032:analysis*
D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; C056934:fumonisin B2; C056933:fumonisin B1