title: Tricholin, a new antifungal agent from Trichoderma viride, and its action in biological control of Rhizoctonia solani.
abstract: Tricholin, a ribosome-inactivating protein isolated from the culture broth of Trichoderma viride, has been shown to exert fungicidal effects on Rhizoctonia solani through a multi-hit kinetic interaction. Tricholin causes a parallel cessation of growth, uptake of amino acids, and protein biosynthesis. The in vivo mode of action of tricholin on protein synthesis and cell growth appears to be attributed to the diminishing of the polysome formation in R. solani through damage to large ribosomal subunits. These results concur with previous data and prove that tricholin is an effective inhibitor of protein synthesis. The efficacy of tricholin as an antibiotic agent was estimated to have a duration of approximately 42 hours.
authors.affiliation: Institute of Genetics, Yang-Ming Medical College, Taipei, Taiwan, ROC.
authors.forename: A
authors.initials: A
authors.lastname: Lin
authors.forename: T M
authors.initials: TM
authors.lastname: Lee
authors.forename: J C
authors.initials: JC
authors.lastname: Rern
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005656:Fungal Proteins / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D007700:Kinetics; D009181:Mycoses / Q000188:drug therapy; D011500:Protein Synthesis Inhibitors; D012232:Rhizoctonia / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects; D014242:Trichoderma / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D005659:Fungicides, Industrial; D011500:Protein Synthesis Inhibitors; C089086:Tricholin protein, Trichoderma viride