[Three dimensional analysis of noninvasive ductal carcinoma of the breast using immunohistochemical staining technique of laminin].
Three dimensional structure of noninvasive ductal carcinoma of the breast was analyzed histopathologically by reconstructing numerous continuous serial sections. About 1,000 continuous serial sections were made and examined microscopically. Immunohistochemical staining of basement membrane was used together in areas where relation between adjoining ducts was delicate. The same procedure was performed for the normal mammary glands. 1) Celloidin-paraffin embedding was useful in immunohistochemical staining on serial sections. It retained delicate morphological structure of tissues even after vigorous protease digestion. 2) In all 3 cases of noninvasive ductal carcinoma, carcinoma cells were surrounded completely with continuous basement membrane. The "intraductal lesions" under the histological sections proved to be the lobule filled with cancer cells and expanded spherically. 3) In normal mammary glands the ducts branched dischotomously without forming any network among them. In all three cases of noninvasive ductal carcinoma, however, network formation by pathological anastomoses between adjoining terminal ductolobular units (TDLU) was confirmed, which suggested structural atypisum of the ducts.
Ishikawa
T
T
First Department of Surgery, Kobe University School of Medicine, Japan.
Yamamoto
K
K
Saito
Y
Y
D000328:Adult; D000368:Aged; D001485:Basement Membrane / Q000737:chemistry / Q000473:pathology; D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry / Q000473:pathology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis*; D008875:Middle Aged; D016612:Paraffin Embedding
D007797:Laminin