Individualisation of two new genomic groups among American Borrelia burgdorferi sensu lato strains.
We developed a quick typing method for Borrelia burgdorferi sensu lato strains using a fla gene-based PCR assay, followed by dot blot hybridization with non-radioactive species-specific probes. Thirty-six out of 46 strains belonged to one of the four described species (B. burgdorferi sensu stricto n = 11, B. garinii n = 11, B. afzelii n = 9 and B. japonica n = 5) and hybridized with its own species-specific probe. Among the 10 remaining American strains, two new additional genomic groups were identified. This finding was confirmed by direct sequencing of the fla gene-derived amplicons and whole DNA hybridization.
Assous
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MV
Laboratoire de Bactériologie, Faculté de Médecine Cochin-Port Royal, Paris, France.
Postic
D
D
Paul
G
G
Névot
P
P
Baranton
G
G
D000818:Animals; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics*; D005408:Flagellin / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D006801:Humans; D008193:Lyme Disease / Q000453:epidemiology / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D005423:Siphonaptera / Q000382:microbiology; D013987:Ticks / Q000382:microbiology; D014481:United States / Q000453:epidemiology
D005408:Flagellin