title: Nucleotide sequence of the coat protein coding region of tulip breaking virus.
abstract: cDNA of tulip breaking virus-tulip (TBV-tulip) RNA was synthesized and cloned in E. coli. One clone that contains a 4.5 kb insert was identified by restriction enzyme analysis, dot immunobinding assay (DIBA), and partial sequencing. Then 1479 nucleotides of the 3'-terminus of the clone were sequenced and revealed that the sequence contains one open reading frame (ORF), followed by an untranslated region of 255 nucleotides and a poly(A) tract. The deduced amino acid sequence was found to include the C terminus of the predicted RNA-dependent RNA polymerase and the coat protein. A glutamine-alanine dipeptide was identified as a putative NIa protease cleavage site at the N terminus of the coat protein.
authors.affiliation: Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohira
authors.forename: S
authors.initials: S
authors.lastname: Namba
authors.forename: M
authors.initials: M
authors.lastname: Miyagawa
authors.forename: T
authors.initials: T
authors.lastname: Kusumi
authors.forename: T
authors.initials: T
authors.lastname: Tsuchizaki
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002213:Capsid / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D036022:Capsid Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary; D010450:Endopeptidases; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017800:Potyvirus / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D018076:DNA, Complementary; D014764:Viral Proteins; D015678:Viral Structural Proteins; C089035:coat protein, Tulip breaking virus; D012324:RNA-Dependent RNA Polymerase; D010450:Endopeptidases; C059992:nuclear inclusion protein a, mosaic viruses