title: Chromosomal localization of the human histone H2A.X gene to 11q23.2-q23.3 by fluorescence in situ hybridization.
abstract: The human histone H2A.X gene is unusual in that its transcripts are alternatively processed to yield two species, one a 0.6-kb replication-linked histone mRNA and the other a 1.6-kb polyadenylated mRNA. The H2A.X gene has been localized by fluorescence in situ hybridization to chromosome 11q23.2-q23.3, away from the known clusters of human histone genes on chromosomes 1, 6, and 12. Assignment to chromosome 11 was substantiated by analysis of human-hamster somatic cell hybrid lines. As this work was being completed, an 89-bps sequence overlap was found between the downstream regions of the H2A.X gene and the recently sequenced hydroxymethylbilane (HMB)-synthase gene. The H2A.X and HMB-synthase genes have an unusual arrangement, being transcribed towards each other with their polyadenylation sites 330 bp apart. In addition the HMB-synthase gene contains constitutive and erythroid specific promoters. K562, an erythroid cell line, was found to contain a high concentration of the 1.6-kb polyadenylated H2A.X mRNA.
authors.affiliation: Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
authors.forename: V S
authors.initials: VS
authors.lastname: Ivanova
authors.forename: D
authors.initials: D
authors.lastname: Zimonjic
authors.forename: N
authors.initials: N
authors.lastname: Popescu
authors.forename: W M
authors.initials: WM
authors.lastname: Bonner
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D006224:Cricetinae; D004587:Electrophoresis, Agar Gel; D006367:HeLa Cells; D006657:Histones / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis; D014577:Uroporphyrinogens / Q000235:genetics
chemical_list: D006657:Histones; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014577:Uroporphyrinogens; C024393:hydroxymethylbilane