Borna disease virus: immunoelectron microscopic characterization of cell-free virus and further information about the genome.
The etiological agent of Borna disease, a persistent virus infection of the central nervous system with differently expressed symptomatology, was morphologically unknown. Here we provide the first convincing data on its phenotypic architecture. Salt-released virus comprising the biological parameters of Koch's postulates has an unsegmented single-stranded RNA. A dense band (1.22 g/cm3) in CsCl contains 90-nm particles which appear to be enveloped and a fraction of 50- to 60-nm particles. Labeling of the virions with neutralizing antisera and colloidal gold conjugates indicates that the 90-nm particles most likely represent the causative agent.
Zimmermann
W
W
Institut für Virologie, Freie Universität Berlin, Berlin, Federal Republic of Germany.
Breter
H
H
Rudolph
M
M
Ludwig
H
H
D000818:Animals; D001717:Birds; D001891:Borna disease virus / Q000235:genetics* / Q000648:ultrastructure*; D002415:Cats; D002460:Cell Line; D002474:Cell-Free System; D004797:Enzyme-Linked Immunosorbent Assay; D016679:Genome, Viral; D006736:Horses; D016253:Microscopy, Immunoelectron / Q000379:methods; D009836:Oligodendroglia; D012367:RNA, Viral / Q000032:analysis / Q000648:ultrastructure*; D012756:Sheep; D014771:Virion / Q000235:genetics / Q000648:ultrastructure
D012367:RNA, Viral