Type I interferon genes in cattle: restriction fragment length polymorphisms, gene numbers and physical organization on bovine chromosome 8.
Multiple, superimposed Type I interferon (IFN) restriction fragments were resolved following 72-92 h of horizontal electrophoresis. Restriction fragment length polymorphisms (RFLPs) for alpha IFN (IFNA), beta IFN (IFNB), omega IFN (IFNW) and trophoblast IFN (IFNT) genes were identified in HindIII, EcoRI and TaqI digestions from 313 cattle. RFLPs with codominant segregation in cattle pedigrees were considered alleles, and 19 distinct polymorphic Type I IFN loci (5 IFNA, 4 IFNB, 8 IFNW and 2 IFNT) were identified. Allele frequencies and observed heterozygosity values were calculated for each locus and several loci were considered highly informative for linkage analysis. Bovine IFN gene numbers (10 IFNA, 6 IFNB, 20 IFNW and 6 IFNT) were estimated from the number of polymorphic loci plus additional monomorphic hybridizing bands present in EcoRI and HindIII digestions. Physical linkage of the Type I IFN gene families on bovine chromosome 8 was demonstrated by pulsed field gel electrophoresis (PFGE). Hybridization of two or more IFN probes to similarly sized PFGE fragments suggested the tentative gene family order: IFNA/IFNW-IFNT-IFNB. These studies provide a basis for the development of more detailed genetic and physical maps of the bovine Type I IFNs.
Ryan
A M
AM
Department of Veterinary Pathobiology, Texas A&M University, College Station 77843-4467.
Womack
J E
JE
D000483:Alleles; D000818:Animals; D001345:Autoradiography; D002417:Cattle / Q000235:genetics*; D002460:Cell Line; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005787:Gene Frequency; D007370:Interferon Type I / Q000235:genetics*; D008297:Male; D005810:Multigene Family; D009693:Nucleic Acid Hybridization / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D015342:DNA Probes; D007370:Interferon Type I