Staurosporine advances interblastomeric flattening of the mouse embryo.
Staurosporine, an inhibitor of protein kinase activity, causes premature intercellular flattening of blastomeres but does not induce their premature polarisation. The flattening induced is calcium dependent, is reversed transiently at mitosis and requires the continuing presence of the drug. Staurosporine also blocks the decompacting effect of phorbol ester on 8-cell embryos.
O'Sullivan
D M
DM
Department of Anatomy, University of Cambridge, UK.
Johnson
M H
MH
McConnell
J M
JM
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001757:Blastomeres / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016764:Cell Polarity / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D019311:Staurosporine
D000470:Alkaloids; D047428:Protein Kinase Inhibitors; D019311:Staurosporine