title: Crystallization of the malonyl coenzyme A-acyl carrier protein transacylase from Escherichia coli.
abstract: The malonyl coenzyme A-acyl carrier protein transacylase, a single polypeptide chain of 358 amino acid residues and a molecular mass of 32 kDa, is a key component of the fatty acid synthase multienzyme complex. The elucidation of its three-dimensional structure will help in the understanding of the molecular basis of the biosynthesis of fatty acids, as well as of polyketides and related biologically active molecules. Three X-ray-quality crystal forms of the Escherichia coli fabD gene product encoding for malonyl coenzyme A-acyl carrier protein transacylase have been obtained using the hanging-drop method and ammonium sulfate as precipitant. Two are tetragonal and each contains two molecules in the asymmetric unit (form I: space group P4(3(1))2(1)2 with a = b = 83.9 A, c = 166.5 A and form II: space group P4 with a = b = 132.64 A, c = 38.85 A), whereas the third form belongs to the hexagonal system and contains one molecule in the asymmetric unit (space group P6(1(5)) with a = b = 68.52 A, c = 117.71 A). In each case, the diffraction pattern extends to approximately 2.0 A resolution using CuK alpha radiation from a rotating anode source.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Serre
authors.forename: L
authors.initials: L
authors.lastname: Swenson
authors.forename: R
authors.initials: R
authors.lastname: Green
authors.forename: Y
authors.initials: Y
authors.lastname: Wei
authors.forename: I I
authors.initials: II
authors.lastname: Verwoert
authors.forename: E C
authors.initials: EC
authors.lastname: Verbree
authors.forename: A R
authors.initials: AR
authors.lastname: Stuitje
authors.forename: Z S
authors.initials: ZS
authors.lastname: Derewenda
mesh_terms: D051080:Acyl-Carrier Protein S-Malonyltransferase; D000217:Acyltransferases / Q000737:chemistry*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D054889:Fatty Acid Synthase, Type II; D008969:Molecular Sequence Data
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D000217:Acyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase; C494968:fabD protein, E coli; D054889:Fatty Acid Synthase, Type II