title: [The production of recombinant polypeptides from the herpes simplex viruses types 1 and 2].
abstract: The genes IE175, IE63, IE68, IE12, UL29 and UL19 of herpes simplex virus types 1 and 2 were cloned. Fragments of these genes were cloned into open reading frame expression vector pEX1-3. Recombinant proteins containing amino acid sequences of ICP4, ICP27, ICP22, ICP47, major DNA-binding protein and major capsid protein were expressed in E. coli cells by fusion with cro-beta-galactosidase proteins.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Zvonarev
authors.forename: K Iu
authors.initials: KIu
authors.lastname: Shatalin
authors.forename: V I
authors.initials: VI
authors.lastname: Klichko
authors.forename: E V
authors.initials: EV
authors.lastname: Karaseva
authors.forename: M N
authors.initials: MN
authors.lastname: Kuliakina
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000276:immunology; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D014709:Vero Cells; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral; D010455:Peptides; D011994:Recombinant Proteins
vernacular_title: Poluchenie rekombinantnykh polipeptidov virusa gerpesa prostogo tipov 1 i 2.