[High-performance liquid chromatographic method for the determination of diclofenac in serum and its pharmacokinetics in healthy volunteers].
A sensitive and rapid high-performance liquid chromatographic method has been developed for the determination of diclofenac in human serum. The average recovery of diclofenac was 98.2% to 102.5%. The relative standard deviation was 1.29% to 4.52%. The calibration curve was linear in the range from 0.2 microgram/ml to 10.0 micrograms/ml with r = 0.9999. The detection limit of the method was 10 ng/ml. The serum drug concentration-time curve exhibited a 1-compartment open model and the mean T1/2 was 2.15 h.
Zhang
S Y
SY
Zhujiang Hospital, First Military Medical University, Guangzhou.
Zou
H Q
HQ
Zhang
Z Y
ZY
Peng
W L
WL
Liu
L Q
LQ
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004008:Diclofenac / Q000097:blood / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D008297:Male
D004008:Diclofenac