Determination of arginase activity in macrophages: a micromethod.
We propose a modification of Schimke's method for urea determination as a valuable micromethod for measuring arginase in activated macrophages. The method exhibits the following advantages: (a) it uses small amounts of samples (approximately 25,000 macrophages per assay); (b) it does not interfere with other related metabolites that are also present in the activated macrophage such as citrulline or arginine; (c) saturating concentrations of the substrate arginine can be used; and (d) it is much more sensitive than Schimke's method and can detect small amounts of urea, in the order of 0.02 mumol.
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Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
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Soler
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D000818:Animals; D001119:Arginase / Q000032:analysis*; D001854:Bone Marrow Cells; D005260:Female; D008262:Macrophage Activation; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D008832:Microchemistry; D014508:Urea / Q000032:analysis
D014508:Urea; D001119:Arginase