Protective effect of Trionyx carapax on CCl4-induced hepatic injury is mediated by uracil.
Oral administration of crude extracts of Trionyx carapax into rats was found to protect the liver from its CCl4-induced injury as judged by morphological and biochemical observations. The active principle was purified from crude extracts of Trionyx carapax by Sephadex G-75, Sephadex G-25, DEAE-cellulose and silica gel column chromatographies. The results of elemental analysis, infrared and mass spectroscopy demonstrated the purified component to be identical to uracil. Upon administration, authentic uracil exhibited the protective effects on CCl4-induced hepatic injury, a pattern identical to or very similar to that observed with crude extracts of Trionyx carapax. Thus it is concluded that uracil is the active component responsible for protection from CCl4-induced hepatic injury.
Kitajima
S
S
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagasaki University, Japan.
Ogawa
N
N
Ariyoshi
T
T
D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D014498:Uracil / Q000302:isolation & purification / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D014498:Uracil; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase