Expression of a nuclear envelope protein recognized by the monoclonal antibody BU31 in lung tumours: relationship to Ki-67 antigen expression.
The production of the murine monoclonal antibody BU31 is described. This antibody identifies a nuclear envelope protein which is expressed in some but not all cells, and which resembles statin, a protein reported to be expressed by non-proliferating cells. BU31 was applied onto frozen sections of a series of 78 lung tumours and the staining patterns were compared with those obtained with Ki-67. There was an inverse correlation between the proportion of tumour nuclei labelled with the two reagents (r = -0.61, 95 per cent confidence intervals -0.73 to -0.45). However, the four neuroendocrine neoplasms were BU31-negative. Squamous cell carcinomas often showed a peripheral distribution of the cells stained positively with Ki-67, whereas BU31 tended to label centrally situated cells. These observations are consistent with the concept that the antigen recognized by BU31 is expressed by non-proliferating cells in these tumours.
Rowlands
D C
DC
Department of Pathology, University of Birmingham, Edgbaston, U.K.
Bunce
C M
CM
Crocker
J
J
Ayres
J G
JG
Johnson
G D
GD
Ling
N
N
Brown
G
G
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D002455:Cell Division; D002467:Cell Nucleus / Q000737:chemistry; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen; D008175:Lung Neoplasms / Q000737:chemistry*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins