D2-dopaminergic agonist quinpirole and 8-bromo-cAMP have opposite effects on Go alpha GTP-binding protein mRNA without changing D2 dopamine receptor mRNA levels in striatal neurones in primary culture.
Long-term coordinated regulations (during development or by agonists and second messenger molecules) of the expression of mRNAs encoding D2-dopamine (DA) receptors and D2 receptor-linked Go alpha proteins have been studied by Northern blot analysis in mouse embryonic striatal neurones in primary culture. During the course of the cell culture, the levels of both mRNAs increased, in conjunction with the maturation of the neurones. When the preparation was treated with the D2-DA agonist quinpirole (5-15 hrs, 10(-4) M), which decreases cAMP in these neurones, the levels of Go alpha mRNAs were enhanced whereas that of the D2 mRNA remained unchanged. Conversely, the Go alpha mRNAs, but not the D2 mRNA, decreased when the neurones were exposed to 8-bromo-cAMP (16 hrs, 10(-6) M). It is concluded that, in these experimental conditions where neurones have not yet established their connexions, the longterm regulation of the membrane transmission of D2-DA signal might implicate mainly the Go alpha encoding gene.
Maus
M
M
Collège de France, Chaire de Neuropharmacologie, INSERM U114, Paris, France.
Vernier
P
P
Valdenaire
O
O
Homburger
V
V
Bockaert
J
J
Glowinski
J
J
Mallet
J
J
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000502:physiology; D015259:Dopamine Agents / Q000494:pharmacology*; D004873:Ergolines / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000187:drug effects* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D019257:Quinpirole; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects*; D017448:Receptors, Dopamine D2 / Q000187:drug effects* / Q000235:genetics
D015259:Dopamine Agents; D004873:Ergolines; D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D019257:Quinpirole; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP; D019204:GTP-Binding Proteins