[The dependence of determining the hemagglutinin content by SRID on the test system used and on the detergent].
The assay systems for single radial immunodiffusion (SRD) represented by standard reagents of antigens and monospecific antisera to A and B influenza virus haemagglutinin and prepared in two different countries were used for elucidation of the influence of monospecific antisera and some ionic and nonionic detergents on the determination of hemagglutinin content in the homologous (antigen and antiserum from the same country) and heterologous (antigen and antiserum from different countries) assay systems. A high specificity of SRD reaction excluding substitution of antiserum from one country for analogous preparation from another as well as detergent interchangeability were shown.
Kastrikina
L N
LN
Lonskaia
N I
NI
D000954:Antigens, Surface / Q000097:blood; D003902:Detergents; D005069:Evaluation Studies as Topic; D006389:Hemagglutinins, Viral / Q000097:blood*; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion / Q000379:methods; D007202:Indicators and Reagents; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D012680:Sensitivity and Specificity
D000954:Antigens, Surface; D003902:Detergents; D006389:Hemagglutinins, Viral; D007106:Immune Sera; D007202:Indicators and Reagents
Zavisimost' opredeleniia soderzhaniia gemaggliutinina metodom ORID ot ispol'zuemoĭ test-sistemy i detergents.