Increase of both transcription and translation activities following separate irradiation of the in vitro system components with He-Ne laser.
To gain insight into the mechanism by which cell irradiation with low power continuous wave He-Ne laser (632.8 nm) causes a general stimulation of biosynthetic properties, separate components of transcription and translation systems were irradiated (energy dose 2 Joules/cm2; laser power 12 mW) with measurements made of in vitro RNA and protein synthesis. In addition, all tested components were investigated with respect to influence of laser irradiation on their conformation, as spectroscopically monitored. In all cases an increase in both transcription and translation activities was found with a significant change in absorbance/fluorescence spectra.
Vacca
R A
RA
Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Italy.
Marra
E
E
Quagliariello
E
E
Greco
M
M
D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006371:Helium; D007834:Lasers; D009356:Neon; D014176:Protein Biosynthesis / Q000528:radiation effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014158:Transcription, Genetic / Q000528:radiation effects*
D012333:RNA, Messenger; D006371:Helium; D009356:Neon; D013762:Tetrahydrofolate Dehydrogenase; D001219:Aspartate Aminotransferases; C081591:bacteriophage T3 RNA polymerase; D012321:DNA-Directed RNA Polymerases