Study of microencapsulated pituitary transplantation. Preparation of the capsule and its property.
Microencapsules for pituitary transplantation was successfully developed with sodium alginate and poly-L-lysine (average molecular weight MW 70,000). The permeability of the capsular membrane (CM) was tested. The results showed that 60% of growth hormone (GH), thyroid stimulating hormone (TSH), prolactin (PRL) have passed through the membrane within 3 hours, and reached equilibrium up to 6 hours, suggesting that pituitary hormones (MW 20,000) could pass through CM freely. Bovine serum albumin (MW 67,000) in 50% could also permeate the CM in 3 hours and reached equilibrium up to 24 hours. While immunoglobulin (Ig, MW > 150,000) did not penetrate the CM completely. This study revealed that the microencapsular membrane we made effected immuno-isolation and could be used for pituitary transplantation.
Chen
Z P
ZP
Department of Neurosurgery, First Affiliated Hospital, Suzhou Medical College.
Bao
Y D
YD
D017690:Cell Transplantation / Q000379:methods*; D006085:Graft Survival; D013006:Growth Hormone / Q000378:metabolism; D006801:Humans; D010539:Permeability; D010902:Pituitary Gland / Q000166:cytology*; D011388:Prolactin / Q000378:metabolism; D013972:Thyrotropin / Q000378:metabolism
D011388:Prolactin; D013972:Thyrotropin; D013006:Growth Hormone