Nikkomycin Z is a specific inhibitor of Saccharomyces cerevisiae chitin synthase isozyme Chs3 in vitro and in vivo.
Nikkomycin Z inhibits chitin synthase in vitro but does not exhibit antifungal activity against many pathogens. Assays of chitin synthase isozymes and growth assays with isozyme mutants were used to demonstrate that nikkomycin Z is a selective inhibitor of chitin synthase 3. The resistance of chitin synthase 2 to nikkomycin Z in vitro is likely responsible for the poor activity of this antibiotic against Saccharomyces cerevisiae.
Gaughran
J P
JP
Molecular Genetic Screen Design, Agricultural Research Division, American Cyanamid, Princeton, New Jersey 08543.
Lai
M H
MH
Kirsch
D R
DR
Silverman
S J
SJ
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001557:Benzenesulfonates / Q000494:pharmacology; D002687:Chitin Synthase / Q000037:antagonists & inhibitors* / Q000235:genetics; D004352:Drug Resistance, Microbial; D005456:Fluorescent Dyes / Q000494:pharmacology; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000235:genetics; D007700:Kinetics; D009154:Mutation; D011741:Pyrimidine Nucleosides / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001557:Benzenesulfonates; D005456:Fluorescent Dyes; D007527:Isoenzymes; D011741:Pyrimidine Nucleosides; C007061:C.I. Fluorescent Brightening Agent 28; C011952:nikkomycin; C002458:polyoxorim; D002687:Chitin Synthase