title: Proliferating cell nuclear antigen (PCNA): expression in samples of human astrocytic gliomas.
abstract: In this retrospective study, 55 astrocytic tumours were stained with monoclonal antibody PC10 which recognizes the proliferating cell nuclear antigen (PCNA) in formalin fixed, paraffin-wax embedded sections. All the cases were graded using the Daumas-Duport system and the PCNA labelling indices (LIs) were correlated with tumour grades. Mean PCNA LI was 4.1% in the grade II, 8.1% in the grade III and 26.1% in the grade IV group. Six juvenile and cerebellar (grade I) astrocytomas had a mean PCNA LI of 5.6%. In general PCNA LI increased with tumour grade, but there was considerable variation of LIs especially in grade IV tumours which often had very high PCNA LIs (up to 72.7%). PC10 may be a useful complementary technique in surgical neuropathology, but the significance of the high LIs, seen in a proportion of glioblastomas, needs to be further determined.
authors.affiliation: Department of Neuropathology, University of Dundee.
authors.forename: T
authors.initials: T
authors.lastname: Revesz
authors.forename: N
authors.initials: N
authors.lastname: Alsanjari
authors.forename: J L
authors.initials: JL
authors.lastname: Darling
authors.forename: F
authors.initials: F
authors.lastname: Scaravilli
authors.forename: D P
authors.initials: DP
authors.lastname: Lane
authors.forename: D G
authors.initials: DG
authors.lastname: Thomas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D001932:Brain Neoplasms / Q000276:immunology* / Q000473:pathology; D002648:Child; D005909:Glioblastoma / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis*; D016612:Paraffin Embedding; D018809:Proliferating Cell Nuclear Antigen; D012189:Retrospective Studies
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen