[Quantitative study on cerebral motor cortex structure of kindling rat induced by coriaria lactone].
Ten male Wistar rats were divided into two groups. The experimental rats were injected with subconvulsive dosage of coriaria lactone (1 mg/kg) intramuscularly every 3.5 days to induce kindling seizures. The controls were injected with normal saline. Cerebral motor cortex of kindling rats was taken at the time between seizures and layer V was compared morphologically with that of the control. The number of neurons and neuroglias was counted from light micrographs. The numerical density and length fraction of boutons on the surface of pyramidal somata were measured from electron micrographs with MIAS 300 type image analyser. The number of synapses in neuropil was counted directly and the areal fraction of various constitute in neuropil was measured stereologically from EM photos. In the experimental rats, the number of neurons remained unchanged and the numerical density and length fraction of boutons on the surface of pyramidal somata showed no change, either. The number of synapses (or presynaptic terminals) decreased significantly in neuropil, but the areal fraction of synaptic terminals had no change and the areal fraction of dendrites and unmyelinated fibers decreased. The areal fraction of myelinated fibers increased significantly. The number of neuroglias and the areal fraction of neuroglial processes increased significantly in the experimental rats. The data suggest that the major morphological changes are manifested as a decline in the pyramidal dendritic components, especially dendritic spines, and the axons with its terminals from superficial neurons in motor cortex layer V of the kindling rats induced by coriaria lactone, besides the neurogliosis which is similar to that occurring in other long-term seizures.
Zeng
Z
Z
Liao
D
D
Wu
L
L
Yu
J
J
D000818:Animals; D004827:Epilepsy / Q000139:chemically induced; D007696:Kindling, Neurologic / Q000187:drug effects; D007783:Lactones; D008297:Male; D009044:Motor Cortex / Q000473:pathology / Q000648:ultrastructure*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D007783:Lactones; C049836:coriaria lactone