Isolation of a goat acetyl-CoA carboxylase complementary DNA and effect of milking frequency on the expression of the acetyl-CoA carboxylase and fatty acid synthase genes in goat mammary gland.
1. Using the polymerase chain reaction we have isolated a partial complementary DNA for goat acetyl-CoA carboxylase which is 90 and 82% homologous to the published rat and chicken complementary DNA sequences, respectively. 2. Frequent milking causes an upregulation of the acetyl-CoA carboxylase and fatty acid synthase genes in goat mammary gland that parallels the increase in the respective enzyme activities. 3. The sequence for goat acetyl-CoA carboxylase is in the EMBL data base, Accession Number Z17803.
Travers
M T
MT
Hannah Research Institute, Scotland, U.K.
Barber
M C
MC
D000103:Acetyl-CoA Carboxylase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D064429:Fatty Acid Synthases / Q000235:genetics*; D015870:Gene Expression; D006041:Goats; D007774:Lactation / Q000502:physiology*; D008321:Mammary Glands, Animal / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017385:Sequence Homology
D004247:DNA; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase