Growth of and aflatoxin production by Aspergillus flavus in peanuts stored under modified atmosphere packaging (MAP) conditions.
The combined effects of water activity (aw), storage temperature, headspace oxygen and carbon dioxide concentrations on the growth of, and aflatoxin production by Aspergillus flavus on sterile peanuts were examined using a process optimization technique termed response surface methodology (RSM). Regression analysis of the data indicated that aw, storage temperature and initial headspace oxygen concentration were all significant factors (P < 0.001) affecting the growth of, and aflatoxin production by A. flavus. Extensive growth and aflatoxin production occurred during the first week of storage in most treatment combinations. Maximum growth occurred in peanuts with an aw of 0.97, a storage temperature of 25 degrees C and headspace oxygen of 10% (balance 60:40 carbon dioxide:nitrogen), after 21 days of storage while maximum aflatoxin production occurred at a lower aw of 0.94, after 21 days under similar storage/gaseous conditions. In several treatment combinations, where high levels of aflatoxin (> 20 ng/g) were initially detected, aflatoxin concentration decreased during storage to levels less than the current regulatory limit of 20 ng/g. This study has shown that A. flavus can grow and produce aflatoxin in carbon dioxide enriched atmospheres in the presence of oxygen. It also emphasizes the combined effect of several 'barriers' to inhibit and reduce aflatoxin in MAP products containing various levels of residual oxygen.
Ellis
W O
WO
Department of Food Science and Agricultural Chemistry, McGill University, Montreal, Canada.
Smith
J P
JP
Simpson
B K
BK
Ramaswamy
H
H
Doyon
G
G
D000348:Aflatoxins / Q000096:biosynthesis*; D010367:Arachis / Q000382:microbiology*; D001231:Aspergillus flavus / Q000254:growth & development* / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D005516:Food Microbiology; D005519:Food Preservation; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D010101:Oxygen Consumption; D012044:Regression Analysis; D013696:Temperature
D000348:Aflatoxins; D002245:Carbon Dioxide; D009584:Nitrogen