bcl-2 expression in epidermal keratinocytic diseases.
BACKGROUND
The bcl-2 protein has been shown to suppress apoptosis, and overexpression of the bcl-2 protein has been reported in several malignant tumors. Skin is one of the largest organs in the body, and the most common human malignancies arise from keratinocytes in the epidermis. In this paper, the authors analyzed immunohistochemically the expression of the bcl-2 protein in several keratinocytic (KC) tumors and inflammatory skin disorders to investigate the role of bcl-2 in the development of benign and malignant skin tumors.


METHODS
Seventy-two frozen tissues from patients with inflammatory KC proliferation (chronic dermatitis [CD] and psoriasis vulgaris [PV]), seborrheic keratosis (SK), carcinoma in situ of KC tumors (actinic keratosis [AK] and Bowen's disease [BD]), basal cell carcinoma (BCC), and squamous cell carcinoma (SCC), 2 SCC cell lines, and 20 normal skin were immunostained with an anti-bcl-2 monoclonal antibody.


RESULTS
Tissue with normal KC, CD, PV, and SK scarcely expressed the bcl-2 protein. Seventy-three percent of tissue with BD, 25% with AK, 67% with BCC, and 100% with SCC showed obvious bcl-2 protein expression. bcl-2 expression of BCC, BD, and SCC was restricted to the involved lesions, and surrounding normal tissue with KCs were bcl-2 negative. Interestingly, tissue with atrophic AK expressed no bcl-2 protein (none of five cases), whereas tissue with hypertrophic AK reacted weakly with the anti-bcl-2 antibody (two of three cases).


CONCLUSIONS
bcl-2 protein expression in patients with KC may be related to tumorigenic proliferation possibly due to enhanced cell survival, but not when inflammatory proliferation of keratinocytes is present.
Nakagawa
K
K
Department of Dermatology, Kobe University School of Medicine, Japan.
Yamamura
K
K
Maeda
S
S
Ichihashi
M
M
D001913:Bowen's Disease / Q000737:chemistry; D002280:Basal Cell Carcinoma / Q000737:chemistry; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D002908:Chronic Disease; D003872:Dermatitis; D006801:Humans; D017492:Keratosis, Seborrheic; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D019253:Proto-Oncogene Proteins c-bcl-2; D011565:Psoriasis; D012867:Skin / Q000737:chemistry*; D012871:Skin Diseases; D012878:Skin Neoplasms / Q000737:chemistry*
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2