title: Affinity biosensors based on preconcentration/voltammetric analysis. Detection of phenothiazine drugs at Langmuir-Blodgett films of tyrosine hydroxylase.
abstract: A new route for operating affinity biosensors based on the voltammetric monitoring of the accumulated guest (analyte) is described. High sensitivity and selectivity accrue from the coupling of the specific receptor binding process and the inherent sensitivity of the preconcentration/pulse-voltammetric scheme. The redox (measurement) process results in dissociation of the receptor-guest complex, thus allowing multiple analytical determinations. The receptor layer also serves as an effective barrier that excludes interfering species. The new concept of preconcentration/voltammetric affinity biosensors is illustrated in connection with the detection of phenothiazine drugs using Langmuir-Blodgett films of their receptor, the enzyme tyrosine hydroxylase. The effect of various experimental variables upon the sensor performance is described.
authors.affiliation: Department of Chemistry, New Mexico State University, Las Cruces 88003.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
authors.forename: A V
authors.initials: AV
authors.lastname: Eremenko
authors.forename: I N
authors.initials: IN
authors.lastname: Kurochkin
authors.forename: M F
authors.initials: MF
authors.lastname: Mineyeva
mesh_terms: D015374:Biosensing Techniques; D010084:Oxidation-Reduction; D010640:Phenothiazines / Q000032:analysis*; D012680:Sensitivity and Specificity; D014446:Tyrosine 3-Monooxygenase / Q000737:chemistry*
chemical_list: D010640:Phenothiazines; D014446:Tyrosine 3-Monooxygenase; C031637:phenothiazine