Use of DNA fingerprinting for accurate typing of Actinobacillus pleuropneumoniae.
DNA fingerprinting was tested as a method for detailed differentiation of 23 strains of the causal agent of porcine pleuropneumonia--Actinobacillus pleuropneumoniae. The set consisted of twelve reference strains, each representing one serotype, and eleven field strains belonging to serotype 9 which occurs most frequently in the Czech Republic. Nine of the reference strains could be differentiated from each other and from serotypes 1, 9 and 11, but a very close relatedness was demonstrated among the latter three by repeated examination. Four DNA types were identified among the twelve serotype 9 strains. The findings are discussed within the context of the production and efficiency of industrial and autogenous vaccines.
Rychlík
I
I
Veterinary Research Institute, Brno.
Bartos
M
M
Sesták
K
K
D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis
D004269:DNA, Bacterial