[IgG RF analysis of synovial fluid of the knee joints in 35 patients with rheumatic disease].
Rheumatoid arthritis (RA) is an autoimmune disease. IgG RF in the synovial fluid is a significant indicator for the pathogenesis and differential diagnosis of this disease. To now, RF tested by latex agglutination belongs to the IgM type whereas, in the rheumatoid synovial fluid, most of immunocomplex molecules are IgG which bind into a complex. IgG RF was determined by ELISA in the synovial fluid of 35 cases of joint disorders, including 12 cases of RA, 7 cases of reactive arthritis (ReA), 5 cases of osteoarthritis (OA) and 11 cases of non-synovitis (non-S) which were regarded as negative control. The upper limit of OD value +2s was 0.5. Using this standard 7 out of 12 cases of RA were positive. 2 of 5 cases of OA were weakly positive, and only one case of ReA was positive. This suggest that this test is valuable in the diagnosis of RA. We have also observed the relation between IgG RF in synovial fluid and X-ray and arthroscopic findings of the same knee joint. In 5 of 7 cases of positive RA, II-III X-ray changes and II-III cartilage destruction arthroscopic findings were noted, suggesting a relationship between IgG RF in synovial fluid and articular damage.
Yu
M
M
PUMC Hospital, CAMS and PUMC, Beijing.
D000293:Adolescent; D000328:Adult; D000368:Aged; D016918:Arthritis, Reactive / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007719:Knee Joint; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism; D000091402:Prohibitins; D012216:Rheumatic Diseases / Q000378:metabolism*; D012217:Rheumatoid Factor / Q000032:analysis*; D013582:Synovial Fluid / Q000276:immunology*
D007074:Immunoglobulin G; C000717331:PHB2 protein, human; D000091402:Prohibitins; D012217:Rheumatoid Factor