Requirement for lymphocytes and resident macrophages in LPS-induced pleural eosinophil accumulation.
In this study we investigated the involvement of inflammatory cells in the pleural accumulation of eosinophils induced by lipopolysaccharide (LPS). Intrathoracic (i.t.) injection of LPS (250 ng/cavity) into rats induced a significant eosinophil accumulation that developed within 24 h, was maximal at 48 h, and returned to control values within 120 h. This eosinophil influx was preceded by a huge neutrophil influx within 4 h and accompanied by a mononuclear cell accumulation between 24 and 48 h. Pretreatment with an antineutrophil monoclonal antibody (RP-3, 2 ml per animal) selectively reduced the number of circulating neutrophils within 8 h but failed to alter the LPS-induced eosinophilia. Similarly, platelet depletion with an anti-rat platelet antiserum did not alter the LPS-induced eosinophil accumulation. Cyclosporine (50 mg/kg, 12 and 2 h before) partially inhibited (51%) the LPS-induced pleural eosinophilia, whereas the eosinophilia was not changed by prior degranulation of pleural mast cells with polymyxin B (10 micrograms/cavity, 24 h before). Moreover, selective depletion of T lymphocytes using an anti-Thy 1.0 monoclonal antibody significantly inhibited the eosinophilia triggered by LPS. The i.t. injection of liposomes containing dichloromethylene diphosphonate significantly reduced (65%) the number of resident macrophages after 5 days. Under this condition, the eosinophil infiltration induced by LPS was completely inhibited. Accordingly, the i.t. injection of supernatant from macrophage monolayers, obtained from the pleural cavities of LPS-injected rats, into naive recipient animals led to a twofold increase in the number of pleural eosinophils. In conclusion, our data suggest an important role for resident macrophages and T lymphocytes in the eosinophil accumulation induced by LPS.
Bozza
P T
PT
Departamento de Fisiologia e Farmacodinâmica, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, Brazil.
Castro-Faria-Neto
H C
HC
Penido
C
C
Larangeira
A P
AP
das Graças
M
M
Henriques
M O
MO
Silva
P M
PM
Martins
M A
MA
dos Santos
R R
RR
Cordeiro
R S
RS
D000818:Animals; D015550:Cell Degranulation / Q000502:physiology; D002631:Chemotactic Factors, Eosinophil / Q000096:biosynthesis; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000502:physiology; D016572:Cyclosporine / Q000494:pharmacology; D004804:Eosinophils / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D007278:Injections, Spinal; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008407:Mast Cells / Q000502:physiology; D051379:Mice; D011657:Pulmonary Eosinophilia / Q000139:chemically induced; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology*
D002631:Chemotactic Factors, Eosinophil; D008070:Lipopolysaccharides; D016572:Cyclosporine