Distribution and turnover of acetaldehyde-modified proteins in liver and blood of ethanol-fed rats.
Previous studies have shown that acetaldehyde (AcH)-modified proteins are formed in the liver and blood of rats fed ethanol-containing diets. In this study we report the application of ELISA techniques to study the subcellular distribution and turnover of AcH-modified proteins in ethanol-fed and control rats. Modified proteins were found in liver mitochondrial, crude membrane and cytosolic fractions, as well as in plasma from ethanol-fed rats. No adducts were detected by our assay in haemolysates from the same animals. The rate of decline of AcH-modified proteins after cessation of ethanol feeding was also examined. Modified liver cytosolic proteins were shown to decline with a half-life of 2.3 weeks, whereas modified plasma adducts declined with a half-life of 4.8 weeks.
Nicholls
R M
RM
Department of Biochemistry, University of Queensland, Australia.
Fowles
L F
LF
Worrall
S
S
de Jersey
J
J
Wilce
P A
PA
D000079:Acetaldehyde / Q000493:pharmacokinetics*; D000437:Alcoholism / Q000097:blood*; D000818:Animals; D001798:Blood Proteins / Q000187:drug effects / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism; D008108:Liver Diseases, Alcoholic / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D001798:Blood Proteins; D000431:Ethanol; D000079:Acetaldehyde