The OLFR1 gene encoding the HGMP07E putative olfactory receptor maps to the 17p13-->p12 region of the human genome and reveals an MspI restriction fragment length polymorphism.
Olfactory receptors are believed to be encoded by an extremely large subfamily of G protein-coupled receptors. A human gene (OLFR1) encoding a member of this family was cloned from a genomic library by cross-hybridization with a gene fragment obtained by the polymerase chain reaction. The nucleotide sequence of a 3.4-kb EcoRI fragment was determined, and the protein sequence was deduced from the single open reading frame. The gene was assigned by in situ hybridization of metaphase chromosomes to the 17p13-->p12 region of the human genome, in proximity to the tumor-suppressor gene encoding p53. When used as a probe on Southern blots under moderately stringent conditions, it hybridizes to at least three closely related genes. A restriction fragment length polymorphism was detected after MspI digestion. Mendelian segregation of the gene was assessed in three CEPH families, and linkage analysis confirmed the localization of the locus.
Schurmans
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S
IRIBHN, Université Libre de Bruxelles, Belgium.
Muscatelli
F
F
Miot
F
F
Mattei
M G
MG
Vassart
G
G
Parmentier
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D005796:Genes; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D018035:Receptors, Odorant; D011984:Sensory Receptor Cells / Q000502:physiology*; D016415:Sequence Alignment; D012903:Smell / Q000502:physiology*
C081139:HGMP07E olfactory receptor; D018035:Receptors, Odorant