title: Effects of an N-methyl-D-aspartate antagonist and a GABAergic antagonist on entorhinal tetanic responses during the early stages of amygdala kindling in rats.
abstract: Changes in synaptic potentials during each train stimulation (tetanic responses) have been suggested to intimately relate to the development of kindling. We examined the effects of an NMDA antagonist, carboxypiperazinephosphonate (CPP), and a GABAergic antagonist, picrotoxin, on entorhinal tetanic responses evoked by train stimuli (10 Hz, 100 pulses) at the developmental stage (seizure stage; 0-2) of amygdala kindling in conscious rats, to clarify the significance of facilitation in tetanic responses and the roles of NMDA and GABA receptors in the development of kindling. Facilitation of tetanic responses was noted as a progressive increase in both amplitude and duration of negative potentials in the tetanic responses, especially during the later half of train pulses (51-100). The negative potential area (mV x ms) of the averaged tetanic responses was used as an estimate of the magnitudes of excitatory synaptic activity in the tetanic responses, and correlated significantly (P < 0.001) with the duration of afterdischarges (AD). CPP (10 mg/kg) reversibly blocked AD in association with a significant decrease (P < 0.05) in the negative potential area. The CPP-sensitive component consisted of a slow negative potential with a duration longer than 60 ms and was greater in the later tetanic responses (51-100) than the earlier ones (1-50). Picrotoxin (2-3 mg/kg), which did not produce convulsions, significantly (P < 0.005) increased the negative potential area in the tetanic responses in association with a reversible decrease in the AD threshold. Although positive potentials ascribable to inhibitory synaptic activity were often negligible in the tetanic responses in controls, picrotoxin further decreased the positive potentials of tetanic responses, if any.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology, Osaka City University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hirayama
authors.forename: R
authors.initials: R
authors.lastname: Murata
authors.forename: S
authors.initials: S
authors.lastname: Matsuura
mesh_terms: D000679:Amygdala / Q000502:physiology*; D000818:Animals; D004594:Electrophysiology; D018756:GABA Antagonists; D007696:Kindling, Neurologic; D008032:Limbic System / Q000187:drug effects / Q000503:physiopathology*; D008297:Male; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors*; D010852:Picrotoxin / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D012640:Seizures / Q000503:physiopathology*
chemical_list: D018756:GABA Antagonists; D016202:N-Methylaspartate; D010852:Picrotoxin; D010879:Piperazines; D016194:Receptors, N-Methyl-D-Aspartate; C050592:3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid