The long term stability of mechlorethamine hydrochloride (nitrogen mustard) ointment measured by HPLC.
HPLC has been applied to determine the stability of mechlorethamine hydrochloride (nitrogen mustard) formulated as an ointment in white soft paraffin (10 mg drug, 50 g paraffin and 1 mL acetone). A new solubilization technique is described for extraction of the drug from the ointment for HPLC analysis which has an extraction efficiency of 76.1% with a coefficient of variation of 10.4%. Stored at 4 degrees C the drug content of the ointment remained stable for at least 84 days and stored at 37 degrees C the drug content remained stable for at least 40 days. In comparison aqueous solutions of mechlorethamine at the same concentration, fully degraded after 4 days. These data will aid hospital pharmacists decide on a standardized protocol for the controlled usage of the ointment which is both safe and cost-effective.
Cummings
J
J
Imperial Cancer Research Fund, Medical Oncology Unit, Western General Hospital, Edinburgh, UK.
MacLellan
A
A
Langdon
S J
SJ
Smyth
J F
JF
D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D008466:Mechlorethamine / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry; D009823:Ointment Bases; D009824:Ointments; D010232:Paraffin
D009823:Ointment Bases; D009824:Ointments; D008466:Mechlorethamine; D010232:Paraffin