Glycolipid and protein profiles in trypanosomatids.
A comparative study of glycolipid and protein composition in Trypanosoma cruzi and non-pathogenic trypanosomatids was carried out using Triton X-114 extraction. Protein profiles were analysed by polyacrylamide gel electrophoresis in sodium dodecyl sulphate (SDS-PAGE), and glycolipids were detected using high-performance thin-layer chromatography (HPTLC). Hydrophilic protein profiles were similar in non-pathogenic protozoa. Endotrypanum schaudinni, Crithidia luciliae and T. mega showed five characteristic protein bands ranging between 30 and 66 kDa. In the hydrophobic phase, a band of 50 kDa was present only in T. mega. Strain-specific protein distribution was detected in T. cruzi clone Dm28c and T. cruzi G and Y strains; clone Dm28c had five typical hydrophilic proteins at between 24 and 45 kDa, the G strain had two bands at 45 kDa in the hydrophilic phase and the Y strain had a major protein band at 24 kDa in both phases. T. dionisii and T. cruzi clone Dm28c showed a characteristic distribution of three hydrophilic proteins of approx. 45 kDa. Qualitative analysis of glycolipid composition showed that the T. cruzi strains and Dm28c clone and T. dionisii had four orcinol-positive spots, whereas in the other non-pathogenic trypanosomatids only three glycolipids were detected.
Branquinha
M H
MH
Departamento de Microbiologia Geral, Universidade Federal do Rio de Janeiro, Brasil.
Bergter
E B
EB
de Meirelles
M N
MN
Vermelho
A B
AB
D000818:Animals; D003421:Crithidia / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006017:Glycolipids / Q000032:analysis* / Q000302:isolation & purification; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000737:chemistry; D015800:Protozoan Proteins / Q000032:analysis* / Q000302:isolation & purification; D014349:Trypanosoma cruzi / Q000737:chemistry; D014351:Trypanosomatina / Q000737:chemistry*
D006017:Glycolipids; D015800:Protozoan Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40