Apoptosis of human primary and metastatic colon adenocarcinoma cell lines in vitro induced by 5-fluorouracil, verapamil, and hyperthermia.
Combined verapamil(V) and hyperthermia(HT) showed a significant decrease in HT29 and SW-620 cell counts by 61.5% and 77.6%, respectively, when compared to control. V in conjunction with 5-FU significantly reduced HT-29 count by 37% in comparison to 5-FU alone, and did not enhance the growth inhibitory effect of 5-FU on SW-620. HT did not enhance the growth inhibition seen with 5-FU treatment alone on HT-29, while this combination significantly decreased SW-620 cell count by 28.9% in comparison to administration of 5-FU alone. Joint administration of V and HT with 5-FU appeared to have a possible synergistic effect and reduced HT-29 count by 90% when compared to control. 5-FU alone or in different combinations with V and HT, as well as combination V+HT, results in apoptosis.
Shchepotin
I B
IB
Department of Surgery, Lombardi Cancer Center, Georgetown University, Washington, D.C. 20007.
Soldatenkov
V
V
Buras
R R
RR
Nauta
R J
RJ
Shabahang
M
M
Evans
S R
SR
D000230:Adenocarcinoma / Q000473:pathology* / Q000556:secondary; D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000473:pathology*; D004247:DNA / Q000378:metabolism; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000494:pharmacology*
D004247:DNA; D014700:Verapamil; D005472:Fluorouracil