Role of nitric oxide (EDRF) in radiocontrast acute renal failure in rats.
This study was undertaken to examine the possible role of endothelium-derived relaxing factor (EDRF), identified as nitric oxide (NO), in the pathogenesis of radiocontrast-induced acute renal failure in rats. Normal and salt-depleted rats were monitored for 60 min or 24 h after radiocontrast administration. The administration of L-arginine to normal rats abolished the immediate decrease in p-aminohippurate clearance (CPAH) and attenuated the decrease in inulin clearance (CIn). The administration of NO synthase inhibitor to the salt-depleted animals resulted in a significantly more pronounced decrease in CPAH compared with both the control and the L-arginine-treated animals. The recovery of CIn 24 h after radiocontrast administration to the salt-depleted rats was significantly better in the L-arginine-treated rats than in either the control or inhibitor-treated groups. The administration of radiocontrast material resulted in a significant decrease in urinary guanosine 3',5'-cyclic monophosphate as well as NO2 + NO3 excretion. This decrease was significantly attenuated by L-arginine. Our results 1) suggest that NO plays a major role in the pathogenesis of radiocontrast-induced acute renal failure and 2) suggest a novel therapeutic approach, i.e., the use of L-arginine in this form of acute renal failure.
Schwartz
D
D
Department of Nephrology, Ichilov Hospital, Tel-Aviv Sourasky Medical Center, Israel.
Blum
M
M
Peer
G
G
Wollman
Y
Y
Maree
A
A
Serban
I
I
Grosskopf
I
I
Cabili
S
S
Levo
Y
Y
Iaina
A
A
D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000503:physiopathology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001794:Blood Pressure; D006152:Cyclic GMP / Q000652:urine; D003973:Diatrizoate; D005260:Female; D009566:Nitrates / Q000652:urine; D009569:Nitric Oxide / Q000502:physiology*; D009573:Nitrites / Q000652:urine; D019335:Nitroarginine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000172:deficiency; D013997:Time Factors
D009566:Nitrates; D009573:Nitrites; D003973:Diatrizoate; D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D012964:Sodium; D006152:Cyclic GMP