Phosphatidylcholine-specific phospholipase D activity is elevated in v-Fps-transformed cells.
Activating the protein-tyrosine kinase of v-Fps results in a rapid increase in diglyceride (DG) in rat fibroblasts. The v-Fps-induced increases in DG were detected only when phospholipids were prelabeled with [3H]-myristate, which is incorporated primarily into phosphatidylcholine (PC). Inhibition of phosphatidic acid (PA) phosphatase (PAP), which converts PA to DG, blocked v-Fps-induced DG production. PA is a primary metabolite of type D phospholipases (PLD). Consistent with these observations, PLD activity was activated in response to the kinase activity of v-Fps. The increased PLD activity was detected only when the cells were prelabeled with the PC-specific [3H]-myristate. These data support the hypothesis that v-Fps-induced DG is derived from PC via the PLD/PAP pathway.
Jiang
Y W
YW
Institute for Biomolecular Structure and Function, Hunter College of the City University of New York, NY 10021.
Song
J
J
Zang
Q
Q
Foster
D A
DA
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002461:Cell Line, Transformed; D004075:Diglycerides / Q000378:metabolism; D004789:Enzyme Activation; D005347:Fibroblasts / Q000378:metabolism; D015681:Fusion Proteins, gag-onc / Q000494:pharmacology*; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D010711:Phosphatidate Phosphatase / Q000037:antagonists & inhibitors; D010712:Phosphatidic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D051381:Rats; D013696:Temperature
D004075:Diglycerides; D015681:Fusion Proteins, gag-onc; D009227:Myristic Acids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D019814:Myristic Acid; D016718:Arachidonic Acid; D011433:Propranolol; D011505:Protein-Tyrosine Kinases; C068415:v-fps oncogene protein, Fujinami sarcoma virus; D010711:Phosphatidate Phosphatase; D010739:Phospholipase D