title: Empirical test of an ion parametric resonance model for magnetic field interactions with PC-12 cells.
abstract: A companion paper describes a predictive ion parametric resonance (IPR) model of magnetic field interactions with biological systems based on a selective relation between the ratio of the flux density of the static magnetic field to the AC magnetic field and the charge-to-mass ratio of ions of biological relevance. Previous studies demonstrated that nerve growth factor (NGF)-stimulated neurite outgrowth (NO) in PC-12 cells can be inhibited by exposure to magnetic fields as a function of either magnetic field flux density or AC magnetic field frequency. The present work examines whether the PC-12 cell response to magnetic fields is consistent with the quasi-periodic, resonance-based predictions of the IPR model. We tested changes in each of the experimentally controllable variables [flux densities of the parallel components of the AC magnetic field (Bac) and the static magnetic field (Bdc) and the frequency of the AC magnetic field] over a range of exposure conditions sufficient to determine whether the IPR model is applicable. A multiple-coil exposure system independently controlled each of these critical quantities. The perpendicular static magnetic field was controlled to less than 2 mG for all tests. The first set of tests examined the NO response in cells exposed to 45 Hz Bac from 77 to 468 mG(rms) at a Bdc of 366 mG. Next, we examined an off-resonance condition using 20 mG Bdc with a 45 Hz AC field across a range of Bac between 7.9 and 21 mG(rms). Finally, we changed the AC frequency to 25 Hz, with a corresponding change in Bdc to 203 mG (to tune for the same set of ions as in the first test) and a Bac range from 78 to 181 mG(rms). In all cases the observed responses were consistent with predictions of the IPR model. These experimental results are the first to support in detail the validity of the fundamental relationships embodied in the IPR model.
authors.affiliation: Health Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711.
authors.forename: C F
authors.initials: CF
authors.lastname: Blackman
authors.forename: J P
authors.initials: JP
authors.lastname: Blanchard
authors.forename: S G
authors.initials: SG
authors.lastname: Benane
authors.forename: D E
authors.initials: DE
authors.lastname: House
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D008274:Magnesium / Q000528:radiation effects; D008280:Magnetics; D008345:Manganese / Q000528:radiation effects; D008954:Models, Biological; D009414:Nerve Growth Factors / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D016716:PC12 Cells; D051381:Rats; D014639:Vanadium / Q000528:radiation effects
chemical_list: D009414:Nerve Growth Factors; D014639:Vanadium; D008345:Manganese; D008274:Magnesium