[The production of recombinant polypeptides from the herpes simplex viruses types 1 and 2].
The genes IE175, IE63, IE68, IE12, UL29 and UL19 of herpes simplex virus types 1 and 2 were cloned. Fragments of these genes were cloned into open reading frame expression vector pEX1-3. Recombinant proteins containing amino acid sequences of ICP4, ICP27, ICP22, ICP47, major DNA-binding protein and major capsid protein were expressed in E. coli cells by fusion with cro-beta-galactosidase proteins.
Zvonarev
A Iu
AIu
Shatalin
K Iu
KIu
Klichko
V I
VI
Karaseva
E V
EV
Kuliakina
M N
MN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000276:immunology; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D014709:Vero Cells; D014776:Virus Cultivation
D004279:DNA, Viral; D010455:Peptides; D011994:Recombinant Proteins
Poluchenie rekombinantnykh polipeptidov virusa gerpesa prostogo tipov 1 i 2.