No association between c-myc amplification and TP53 mutation in sarcoma tumorigenesis.
Oncogenes and tumor suppressor genes coparticipate in sarcoma tumorigenesis. We tested 43 sarcomas for c-myc amplification by Southern blot and molecular hybridization techniques and TP53 mutations by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique and direct sequencing of the PCR products. We found eight tumors with c-myc amplification and five different tumors with TP53 mutations but no tumors harbor both c-myc and TP53 alterations. We suggest that c-myc amplification and TP53 mutations do not seem to coparticipate in the tumorigenesis of sarcomas.
Castresana
J S
JS
Department of Tumor Pathology, Karolinska Hospital, Stockholm, Sweden.
Barrios
C
C
Gómez
L
L
Kreicbergs
A
A
D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D005091:Exons; D005784:Gene Amplification; D016259:Genes, myc / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012509:Sarcoma / Q000235:genetics*