Escherichia coli mutY-dependent mismatch repair involves DNA polymerase I and a short repair tract.
Repair of heteroduplex DNA containing an A/G mismatch in a mutL background requires the Escherichia coli mutY gene function. The mutY-dependent in vitro repair of A/G mismatches is accompanied by repair DNA synthesis on the DNA strand bearing mispaired adenines. The size of the mutY-dependent repair tract was measured by the specific incorporation of alpha-[32P]dCTP into different restriction fragments of the repaired DNA. The repair tract is shorter than 12 nucleotides and longer than 5 nucleotides and is localized to the 3' side of the mismatched adenine. This repair synthesis is carried out by DNA polymerase I.
Tsai-Wu
J J
JJ
Department of Biological Chemistry, School of Medicine, University of Maryland at Baltimore 21201.
Lu
A L
AL
D001483:Base Sequence; D045647:DNA Glycosylases; D004256:DNA Polymerase I / Q000378:metabolism*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*
D004269:DNA, Bacterial; D004256:DNA Polymerase I; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; C061738:mutY adenine glycosylase