title: Interaction of H2O2 and dithiothreitol and the genesis of cellular damage in the perfused rat heart.
abstract: 1. H2O2 (0.3 mM) causes release of creatine kinase (CK) from the perfused rat heart, but only when activated by removal of extracellular Ca2+. 2. Dithiothreitol (DTT), 2 mM, provides partial protection against this damage induced by artificially-introduced O2 radicals. 3. Paradoxically, DTT triggers CK release by H2O2 in the presence of extracellular Ca2+. The interaction of these agents in stimulating and protecting against this damage to the sarcolemma is discussed.
authors.affiliation: Department of Environmental and Evolutionary Biology, University of Liverpool, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Daniels
authors.forename: C J
authors.initials: CJ
authors.lastname: Duncan
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology*; D004357:Drug Synergism; D005609:Free Radicals; D006321:Heart / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009206:Myocardium / Q000166:cytology / Q000473:pathology; D010100:Oxygen / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005609:Free Radicals; D006861:Hydrogen Peroxide; D003402:Creatine Kinase; D010100:Oxygen; D004229:Dithiothreitol