Retention of hamster oolemma fusibility with spermatozoa after various enzyme treatments: a search for the molecules involved in sperm-egg fusion.
The plasma membrane (oolemma) of the hamster egg retains the ability to fuse with spermatozoa even after exhaustive treatment with proteases and glycosidases. In contrast, when mouse oolemma is treated with proteases, the ability of eggs to fuse with spermatozoa is reduced. In the present study, similar treatments effective in reducing fusibility in the mouse were reexamined in the hamster. Of the several enzymes and treatments tested, only trypsin in Ca(2+)-free medium significantly reduced the hamster oolemma's ability to fuse with spermatozoa. This is suggestive of a cadherin-like system of binding and fusion. When hamster oolemmae were treated with the same protease regimen that reduced fusibility of mouse oolemma for mouse spermatozoa, heterologous fusion of hamster oolemmae with mouse spermatozoa was reduced, without affecting the fusion of these oolemmae with hamster spermatozoa. These data suggest that a protease-sensitive oolemma molecule is of critical importance for mouse sperm-oolemma fusion but not for hamster sperm-oolemma fusion.
Ponce
R H
RH
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822.
Yanagimachi
R
R
Urch
U A
UA
Yamagata
T
T
Ito
M
M
D000818:Animals; D015820:Cadherins / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002808:Chondroitin Lyases / Q000494:pharmacology; D006224:Cricetinae; D010450:Endopeptidases / Q000494:pharmacology; D005260:Female; D006026:Glycoside Hydrolases / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008561:Membrane Fusion / Q000187:drug effects / Q000502:physiology; D008647:Mesocricetus; D051379:Mice; D010063:Ovum / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D013045:Species Specificity; D013084:Sperm-Ovum Interactions / Q000187:drug effects / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology
D015820:Cadherins; D006026:Glycoside Hydrolases; D010450:Endopeptidases; D014357:Trypsin; D002808:Chondroitin Lyases