Production of bioactive rabbit antibodies against the mutated receptor peptide TSH-R-HIS17 and detection of hypertriiodothyroninemia in the rabbit.
We produced rabbit antibodies against mutated thyrotropin (TSH) receptor peptide TSH-R-HIS17 in comparison with non-mutated TSH receptor peptide TSH-R-ASP17. All the antibodies raised against TSH-R-HIS17 showed thyroid stimulating antibody (TSAb), thyroid stimulation blocking antibody (TSBAb) and/or TSH binding inhibiting immunoglobulin (TBII) activities and also those serum T3 levels were all in thyrotoxic levels associated with TSAb activities. However, all the antibodies raised against TSH-R-ASP17 had no activities of TSAb, TSBAb and/or TBII and those serum T3 levels were all within normal range. The data suggest that the mutation in the TSH receptor gene may have a possible relevance for the pathogenesis of Graves' disease.
Hirooka
Y
Y
Fourth Department of Internal Medicine, Aichi Medical University, Japan.
Mitsuma
T
T
Nogimori
T
T
Ishizuki
Y
Y
Sakai
J
J
Naruse
M
M
Fukayama
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid / Q000235:genetics / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis* / Q000502:physiology; D006639:Histidine / Q000235:genetics / Q000276:immunology; D018828:Immunoglobulins, Thyroid-Stimulating; D008969:Molecular Sequence Data; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D011989:Receptors, Thyrotropin / Q000235:genetics* / Q000276:immunology; D014284:Triiodothyronine / Q000097:blood*
D001323:Autoantibodies; D018828:Immunoglobulins, Thyroid-Stimulating; D010446:Peptide Fragments; D011989:Receptors, Thyrotropin; D014284:Triiodothyronine; D001224:Aspartic Acid; D006639:Histidine