title: Deletion of fowlpox virus homologues of vaccinia virus genes between the 3 beta-hydroxysteroid dehydrogenase (A44L) and DNA ligase (A50R) genes.
abstract: A fragment of 4156 bp of fowlpox virus (FPV) genomic DNA contains homologues of vaccinia virus 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD; A44L) and DNA ligase (A50R) genes. The FPV locus has clearly been rearranged relative to that of vaccinia virus as homologues of genes A45R to A49R, including the thymidylate kinase and a gene with homology to superoxide dismutase, are deleted. The deleted genes are replaced by two open reading frames: for a serine proteinase inhibitor with homology to vaccinia virus gene K2L and for a protein with no significant homology to proteins in the databases. In addition, the FPV homologues of A44L and A50R are in the same polarity in FPV whereas they are in opposite polarities in vaccinia virus. Increased 3 beta-HSD activity has been demonstrated in cells infected with either of two different strains of FPV or with canarypox virus.
authors.affiliation: Institute for Animal Health, Compton Laboratory, Nr. Newbury, Berkshire, U.K.
authors.forename: M A
authors.initials: MA
authors.lastname: Skinner
authors.forename: J B
authors.initials: JB
authors.lastname: Moore
authors.forename: M M
authors.initials: MM
authors.lastname: Binns
authors.forename: G L
authors.initials: GL
authors.lastname: Smith
authors.forename: M E
authors.initials: ME
authors.lastname: Boursnell
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000595:Amino Acid Sequence; D001665:Binding Sites; D011088:DNA Ligases / Q000235:genetics*; D005587:Fowlpox virus / Q000201:enzymology / Q000235:genetics*; D017353:Gene Deletion; D005814:Genes, Viral; D016679:Genome, Viral; D008239:Lysine; D008969:Molecular Sequence Data; D009703:Nucleoside-Phosphate Kinase / Q000235:genetics; D016366:Open Reading Frames; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000235:genetics; D014616:Vaccinia virus / Q000201:enzymology / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D015678:Viral Structural Proteins; D015096:3-Hydroxysteroid Dehydrogenases; D013482:Superoxide Dismutase; D009703:Nucleoside-Phosphate Kinase; C020093:dTMP kinase; D011088:DNA Ligases; D008239:Lysine