Determination of rhoifolin and daidzin in human plasma by high-performance liquid chromatography.
A method for determining flavonoids in human plasma is presented for application to pharmacokinetic studies of two flavonoids, rhoifolin and daidzin. Isocratic reversed-phase high-performance liquid chromatography (HPLC) was used with genistin as an internal standard and solid-phase extraction using a Sep-Pak C18 cartridge. The mobile phases were acetonitrile-0.1 M ammonium acetate solution (20:80, v/v) for rhoifolin and methanol-0.1 M ammonium acetate solution (33:67, v/v) for daidzin. The detection limits on-column were 2 ng for rhoifolin and 0.5 ng for daidzin.
Ishii
K
K
Kyorin University, School of Health Sciences, Tokyo, Japan.
Urano
S
S
Furuta
T
T
Kasuya
Y
Y
D000085:Acetates; D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004187:Disaccharides / Q000097:blood* / Q000493:pharmacokinetics; D005419:Flavonoids / Q000097:blood* / Q000493:pharmacokinetics; D006027:Glycosides; D006801:Humans; D007529:Isoflavones / Q000097:blood* / Q000493:pharmacokinetics; D005947:Glucose / Q000031:analogs & derivatives; D008358:Mannose / Q000031:analogs & derivatives; D047310:Apigenin
D000085:Acetates; D000097:Acetonitriles; D004187:Disaccharides; D005419:Flavonoids; D006027:Glycosides; D007529:Isoflavones; C040641:genistin; C013908:daidzin; C089378:rhoifolin; D005947:Glucose; D008358:Mannose; D047310:Apigenin; C018824:ammonium acetate; C032159:acetonitrile