title: An explanation for lagging strand replication: polymerase hopping among DNA sliding clamps.
abstract: The replicase of E. coli, DNA polymerase III holoenzyme, is tightly fastened to DNA by its ring-shaped beta sliding clamp. However, despite being clamped to DNA, the polymerase must rapidly cycle on and off DNA to synthesize thousands of Okazaki fragments on the lagging strand. This study shows that DNA polymerase III holoenzyme cycles from one DNA to another by a novel mechanism of partial disassembly of its multisubunit structure and then reassembly. Upon completing a template, the polymerase disengages from its beta clamp, hops off DNA, and reassociates with another beta clamp at a new primed site. The original beta clamp is left on DNA and may be harnessed by other machineries to coordinate their action with chromosome replication.
authors.affiliation: Microbiology Department, Cornell University Medical College, New York, New York 10021.
authors.forename: P T
authors.initials: PT
authors.lastname: Stukenberg
authors.forename: J
authors.initials: J
authors.lastname: Turner
authors.forename: M
authors.initials: M
authors.lastname: O'Donnell
mesh_terms: D004247:DNA; D004249:DNA Damage; D004258:DNA Polymerase III / Q000378:metabolism*; D017931:DNA Primers / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D046911:Macromolecular Substances; D008957:Models, Genetic; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D046911:Macromolecular Substances; C022262:Okazaki fragments; D004247:DNA; D004258:DNA Polymerase III