title: Methylglyoxal and cell viability.
abstract: Methylglyoxal by depleting glutathione stores increased Trypan-blue uptake by the cells incubated in glucose, pyruvate and amino acids free medium. Only a transient fall of glutathione concentration without any effect on cell viability was caused by methylglyoxal when the medium was supplemented with above-mentioned compounds. The role of gamma-glutamyl-transpeptidase is discussed.
authors.affiliation: Semmelweis University Medical School, First Institute of Biochemistry, Budapest, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Braun
authors.forename: T
authors.initials: T
authors.lastname: Garzó
authors.forename: P
authors.initials: P
authors.lastname: Riba
authors.forename: J
authors.initials: J
authors.lastname: Mandl
authors.forename: M P
authors.initials: MP
authors.lastname: Kalapos
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011765:Pyruvaldehyde / Q000494:pharmacology*; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid; D014343:Trypan Blue / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003470:Culture Media; D011773:Pyruvates; D011765:Pyruvaldehyde; D019289:Pyruvic Acid; D005978:Glutathione; D014343:Trypan Blue; D005947:Glucose