Retinoids regulate expression of the retinol-binding protein gene in hepatoma cells in culture.
The expression of the gene coding for retinol-binding protein has been studied in a system of cultured human hepatoma cells exposed to retinoids. We report that the gene is positively modulated by retinol and retinoic acid in a time- and dose-dependent fashion. The stimulation at the mRNA level is paralleled by an increase of the corresponding protein that is secreted in the presence of the physiological ligand. An RBP-CAT chimeric gene, introduced by transfection, is also responsive to the treatment, showing the gene dose-dependency as the endogenous gene. These results demonstrate that retinoids up-regulate the RBP gene and that the control takes place at transcriptional level.
Mourey
M S
MS
Dipartimento di Biochimica e Biotecnologie Mediche, Facolta' di Medicina e Chirurgia, Universita' degli Studi di Napoli Federico II, Italy.
Quadro
L
L
Panariello
L
L
Colantuoni
V
V
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D012176:Retinoids / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014801:Vitamin A / Q000494:pharmacology
D012333:RNA, Messenger; D012176:Retinoids; D012177:Retinol-Binding Proteins; D014801:Vitamin A; D014212:Tretinoin; D015500:Chloramphenicol O-Acetyltransferase