title: Redistribution of intracellular Ca2+ stores during phagocytosis in human neutrophils.
abstract: Subcellular gradients of cytosolic free Ca2+ concentration, [Ca2+]i, are thought to be critical for the localization of functional responses within a cell. A potential but previously unexplored mechanism for the generation of gradients of [Ca2+]i is the accumulation of Ca2+ stores at the site of Ca2+ action. The distribution of the Ca2+ store markers Ca(2+)-dependent adenosine triphosphatase and calreticulin was investigated in resting and phagocytosing human neutrophils. Both proteins showed an evenly distributed fine granular pattern in nonphagocytosing cells, but became markedly concentrated in the filamentous actin-rich cytoplasmic area around the ingested particle during phagocytosis. This redistribution began at early stages of phagocytosis and did not depend on an increase in [Ca2+]i. Thus, accumulation of Ca2+ stores in a restricted area of the cell may contribute to the generation of localized increases in [Ca2+]i.
authors.affiliation: Department of Medical Microbiology, Linköping University, Sweden.
authors.forename: O
authors.initials: O
authors.lastname: Stendahl
authors.forename: K H
authors.initials: KH
authors.lastname: Krause
authors.forename: J
authors.initials: J
authors.lastname: Krischer
authors.forename: P
authors.initials: P
authors.lastname: Jerström
authors.forename: J M
authors.initials: JM
authors.lastname: Theler
authors.forename: R A
authors.initials: RA
authors.lastname: Clark
authors.forename: J L
authors.initials: JL
authors.lastname: Carpentier
authors.forename: D P
authors.initials: DP
authors.lastname: Lew
mesh_terms: D000199:Actins / Q000032:analysis; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D037282:Calreticulin; D005455:Fluorescent Antibody Technique; D006801:Humans; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism / Q000648:ultrastructure; D012261:Ribonucleoproteins / Q000378:metabolism
chemical_list: D000199:Actins; D015220:Calcium Channels; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D012261:Ribonucleoproteins; D000252:Calcium-Transporting ATPases; D002118:Calcium