title: [The isolation and study of the characteristics of a cytopathic strain of the hepatitis A virus].
abstract: A fast-growing cytopathic isolate of human hepatitis A virus (strain MB-7) was derived from fecal samples of infected patients and adapted to growth in FRhK-4 cell culture. A positive serum standard against HAV and electron microscopy were used to demonstrate that MB-7 belonged to human hepatitis A virus. The strain MB-7 induced plaque formation in FRhK-4 under agar overlay after 10-12 days of incubation. The PCR products of gene VP1 were cloned in E. coli and its primary structure was determined. MB-7 was shown to have more homology with HAV strains isolated in the USA and China.
authors.forename: A G
authors.initials: AG
authors.lastname: Maĭdaniuk
authors.forename: G I
authors.initials: GI
authors.lastname: Tiunnikov
authors.forename: V E
authors.initials: VE
authors.lastname: Konakova
authors.forename: E P
authors.initials: EP
authors.lastname: Bondarenko
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Nemtsov
authors.forename: L G
authors.initials: LG
authors.lastname: Karpovich
authors.forename: T V
authors.initials: TV
authors.lastname: Kalashnikova
authors.forename: N V
authors.initials: NV
authors.lastname: Mamaeva
authors.forename: S V
authors.initials: SV
authors.lastname: Sosnovtsev
authors.forename: V E
authors.initials: VE
authors.lastname: Chizhikov
mesh_terms: D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D003588:Cytopathogenic Effect, Viral; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D006506:Hepatitis A / Q000382:microbiology; D006507:Hepatovirus / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity*; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012800:Siberia; D014776:Virus Cultivation
chemical_list: D000956:Antigens, Viral; D018076:DNA, Complementary; D004279:DNA, Viral
vernacular_title: Vydelenie i izuchenie kharakteristik tsitopaticheskogo shtamma virusa gepatita A.