Characterization of two novel filamentous phages of Xanthomonas.
Two filamentous phages of Xanthomonas campestris pv. vesicatoria and Xanthomonas oryzae pv. oryzae were isolated and designated phi Xv and phi Xo, respectively. They were similar to other filamentous phages of Xanthomonas in (i) shape, (ii) restrictive host specificity, (iii) high stability, (iv) an ssDNA genome, (v) a dsDNA as the replicative form (RF), (vi) propagation without lysis of host cells and (vii) ability to integrate into the host chromosome. These phages showed sequence homology to filamentous phage phi Lf of X. c. pv. campestris. phi Xv was inactivated by antisera against phi Xv, phi Xo and phi Lf, whereas phi Xo and phi Lf were inactivated only by their respective antisera and the anti-phi Xv serum. Both the single-stranded phage DNAs and the RF DNAs of phi Xv, phi Xo and phi Lf were able to transfect X. c. pv. vesicatoria, X. o. pv. oryzae and X. c. pv. campestris. Physical maps of phi Xv and phi Xo were constructed for the RF DNAs. Genome sizes were estimated, based on mapping data, to be 6.8 kb for phi Xv and 7.6 kb for phi Xo, larger than that of the phi Lf genome (6.0 kb). The difference in genome sizes appeared to result from insertions of large DNA fragments. These fragments and the regions mediating integration were localized in the physical maps.
Lin
N T
NT
Department of Botany, National Chung Hsing University, Taichung, Taiwan, Republic of China.
You
B Y
BY
Huang
C Y
CY
Kuo
C W
CW
Wen
F S
FS
Yang
J S
JS
Tseng
Y H
YH
D001435:Bacteriophages / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism; D007106:Immune Sera; D008854:Microscopy, Electron; D016366:Open Reading Frames; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014779:Virus Replication; D014974:Xanthomonas; D016959:Xanthomonas campestris
D004279:DNA, Viral; D007106:Immune Sera