Mixed-mode fluorescent DNA sequencing.
Fluorescence-based, automated DNA sequences represent one of the major advances in recent molecular biology. Two main technologies have been developed in this field: the single-label/four-lane system and the four-label/one-lane system. The following present the use of single-label-sequencing chemistry, which resembles traditional radioactive DNA sequencing, using the four-label system ABI 373A that expands its flexibility and obtains data that are immediately interpretable without software manipulation. This method has been named mixed-mode fluorescent DNA sequencing. Here we show one of its possible applications in molecular genetic analysis.
De Bellis
G
G
Consiglio Nazionale delle Ricerche Istituto di Tecnologie Biomediche Avanzate, Milano, Italy.
Consani
I
I
Caramenti
G
G
Pergolizzi
R
R
Debernardi
S
S
Invernizzi
L
L
Luzzana
M
M
D001322:Autoanalysis; D001483:Base Sequence; D005456:Fluorescent Dyes; D005914:Globins / Q000235:genetics; D006455:Hemoglobins, Abnormal / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software
D005456:Fluorescent Dyes; D006455:Hemoglobins, Abnormal; D011933:Reagent Kits, Diagnostic; D005914:Globins