Sensitivity of AMPA receptors to pentobarbital.
The inhibitory effects of pentobarbital on various AMPA receptors expressed (GluR1, GluR3, GluR1/3, GluR1/2, and GluR2/3) in Xenopus oocytes were examined. Combinations of AMPA receptor subunits that included GluR2 demonstrated a much higher sensitivity to blockade by this barbiturate and the apparent co-operativity of the interaction of pentobarbital with the receptor was reduced. This evidence demonstrates that the GluR2 subunit alters the structure of AMPA receptors in such a way as to facilitate any interaction with this barbiturate.
Taverna
F A
FA
Department of Physiology, Faculty of Medicine, University of Toronto, Ont., Canada.
Cameron
B R
BR
Hampson
D L
DL
Wang
L Y
LY
MacDonald
J F
JF
D000818:Animals; D004305:Dose-Response Relationship, Drug; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D010424:Pentobarbital / Q000494:pharmacology*; D018091:Receptors, AMPA / Q000187:drug effects* / Q000378:metabolism; D017470:Receptors, Glutamate / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014982:Xenopus laevis
D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D011994:Recombinant Proteins; D010424:Pentobarbital