title: Whole blood clot lysis: enhanced by exposure to autologous but not to homologous plasma.
abstract: A new phenomenon is described: Whole blood clots lyse faster in the plasma of the same donor than in another donor's plasma. We have confirmed this finding in 68 healthy volunteers by a standardized, pair-wise analysis and have found a mean difference in clot weights of 8.8 +/- 0.99% (SEM, p < 0.0001) after 6 h of urokinase-induced (200 U/ml) clot lysis. No difference was found in a group of 7 pairs of identical twins. Further analysis revealed that increasing concentrations of platelets in the plasma reduced the difference significantly but did not abolish it. A 1:1 mixture of autologous with homologous plasma reduced the autologous advantage by almost 50%, thus making an inhibitor unlikely. The absence of cellular components in clots of platelet-poor plasma resulted in the loss of the advantage after 2 h of lysis, but not in the early phase. We conclude that there is a clear advantage of autologous over homologous clot lysis. Potential mechanisms are discussed and include an increased affinity of enzymes for their substrates in a given individual.
authors.affiliation: University Hospital of Bern, Dept. of Medicine, Switzerland.
authors.forename: J H
authors.initials: JH
authors.lastname: Beer
authors.forename: H P
authors.initials: HP
authors.lastname: Kläy
authors.forename: T
authors.initials: T
authors.lastname: Herren
authors.forename: A
authors.initials: A
authors.lastname: Haeberli
authors.forename: P W
authors.initials: PW
authors.lastname: Straub
mesh_terms: D000293:Adolescent; D000328:Adult; D001782:Blood Donors; D001804:Blood Transfusion, Autologous; D005069:Evaluation Studies as Topic; D005260:Female; D005342:Fibrinolysis / Q000502:physiology*; D006801:Humans; D008297:Male; D010949:Plasma / Q000502:physiology*; D013927:Thrombosis / Q000628:therapy*; D014430:Twins, Monozygotic