Protein kinase C-dependent expression of type I transglutaminase mRNA in ganglioside GQ1b- and calcium-stimulated human keratinocytes.
Ganglioside GQ1b was found to induce terminal differentiation in normal epidermal keratinocytes (Yada et al. (1991) Biochem. J. 279, 665-670). In GQ1b-stimulated human keratinocytes, the increase in mRNA expression of Type I TGase preceded the increases in the TGase activity. The enhancement of its mRNA expression was inhibited when protein kinase C (PKC) was suppressed by pretreatment with an inhibitor, H-7, or down-regulation by long-term preincubation with phorbol dibutyrate. These results indicate that PKC is involved in the activation of TGase probably via gene expression of TGase mRNA in GQ1b-induced human keratinocyte differentiation. Similar results were also obtained in Ca(2+)-induced differentiation.
Yada
Y
Y
Department of Biochemistry, Gifu University School of Medicine, Japan.
Polakowska
R R
RR
Okano
Y
Y
Nozawa
Y
Y
D002118:Calcium / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005732:Gangliosides / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology*; D011493:Protein Kinase C / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D011503:Transglutaminases / Q000235:genetics*
D005732:Gangliosides; D012333:RNA, Messenger; C039129:GQ1b ganglioside; D011503:Transglutaminases; D011493:Protein Kinase C; D002118:Calcium