title: Direct PCR detection of foot-and-mouth disease virus.
abstract: A PCR assay for the detection and characterization of foot-and-mouth disease virus was developed. The procedure allows RT-PCR amplification following direct adsorption of viral suspensions to microtiter plates, avoiding previous steps of phenol-extraction or heating. Using this procedure, FMDV-specific (based on 3D gene sequences), as well as serotype-specific (based on VP1 gene sequences) amplification were achieved for viral samples of serotypes A, O and C, either from cell culture supernatants or from lesions of infected animals. The assay allowed detection of around 15 PFU, being 500-fold more sensitive than a conventional indirect ELISA. This new method constitutes a simple, rapid and efficient alternative for the diagnosis and characterization of FMDV by PCR.
authors.affiliation: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez
authors.forename: J I
authors.initials: JI
authors.lastname: Núñez
authors.forename: G
authors.initials: G
authors.lastname: Nolasco
authors.forename: F
authors.initials: F
authors.lastname: Ponz
authors.forename: F
authors.initials: F
authors.lastname: Sobrino
authors.forename: C
authors.initials: C
authors.lastname: de Blas
mesh_terms: D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000302:isolation & purification*; D005536:Foot-and-Mouth Disease / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods