title: Cloning and sequence analysis of murine cytomegalovirus protease and capsid assembly protein genes.
abstract: The murine cytomegalovirus UL80 open reading frame was cloned and the predicted amino acid sequence compared with those from other herpesviruses. The open reading frame encodes a fused protease-capsid assembly protein precursor and maintains conserved features including the active site serine, conserved regions CD1 through CD5, the release and maturation sites, and a potential internal cleavage site within the protease. However, the murine cytomegalovirus protease differs in comparison with the other proteases because it contains a unique 15-16 amino acid insertion between CD3 and CD1. The assembly protein sequences are relatively divergent, but they can be arranged into groups defined by herpesvirus subfamily, with each group possessing a conserved motif at its carboxyl terminus.
authors.affiliation: Department of Pathology, St. Louis University Health Sciences Center, MO 63104.
authors.forename: J M
authors.initials: JM
authors.lastname: Loutsch
authors.forename: N J
authors.initials: NJ
authors.lastname: Galvin
authors.forename: M L
authors.initials: ML
authors.lastname: Bryant
authors.forename: B C
authors.initials: BC
authors.lastname: Holwerda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003587:Cytomegalovirus / Q000235:genetics*; D010450:Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012367:RNA, Viral / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D012367:RNA, Viral; D010450:Endopeptidases