Effect of dietary vitamin E on L-[3H]glutamate binding in rabbit cerebellum.
To demonstrate the role of vitamin E on cerebellar function, studies on rabbits fed low and high levels of dietary vitamin E were performed. The L-[3H]glutamate binding to cerebellar membranes of rabbits fed normal, high and low vitamin E diet showed receptor density (Bmax) values (mean +/- SEM) of 274 +/- 13, 637 +/- 37, and 265 +/- 60 pmol/mg protein, respectively, and dissociation equilibrium constant (KD) values of 257 +/- 99, 233 +/- 77, and 120 +/- 15 nM, respectively. Significant difference of Bmax from control was observed in the high dietary vitamin E group and of KD from control for the low dietary vitamin E group. These results indicate that dietary vitamin E levels have demonstrable effects on the central nervous system, especially the glutamate neurotransmitter system in rabbit cerebellum.
Kittiwatanapaisan
W
W
Neuro- and Behavioural Biology Center, Mahidol University, Nakornpathom, Thailand.
Wilairat
P
P
Kotchabhakdi
N
N
Govitrapong
P
P
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D004032:Diet; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D011817:Rabbits; D017470:Receptors, Glutamate / Q000187:drug effects / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*
D005971:Glutamates; D017470:Receptors, Glutamate; D014810:Vitamin E; D018698:Glutamic Acid