Structure, genetic mapping, and expression of the mouse Hgf/scatter factor gene.
The cytokine termed hepatocyte growth factor or scatter factor (HGF/SF) has been implicated in embryonic development and liver regeneration. Mouse HGF/SF cDNA clones were obtained by screening a mouse liver cDNA library with synthetic oligonucleotides, as well as by using the method of reverse transcription-polymerase chain reaction (RT-PCR) with mRNA isolated from adult mouse brain. Sequence analysis of mouse HGF/SF cDNA clones spanning the entire coding region revealed an overall amino acid identity of 90% and 98% with human and rat HGF, respectively. Using mouse HGF antisense RNA as a probe, we observed the expression of HGF/SF mRNA in the liver, kidney, whole brain and cerebellum of adult mice as well as in day 9 and day 10 mouse embryos. The mouse HGF/SF cDNA was utilized for mapping Hgf to the centromeric region of mouse Chromosome 5 in apparent close proximity to the reeler mutation by the analysis of two multilocus crosses. The relationship of Hgf to the reeler mutation is discussed. The availability of mouse HGF/SF cDNA clones should facilitate further analysis of HGF/SF function during development.
Lee
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CC
Laboratory of Developmental Biology, National Institute of Dental Research, NIH, Bethesda, MD 20892.
Kozak
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CA
Yamada
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KM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism; D015870:Gene Expression; D017228:Hepatocyte Growth Factor / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; D017228:Hepatocyte Growth Factor; D012260:Ribonucleases