Deleterious effects of disulfiram on the respiratory electron transport system of liver mitochondria.
1. The mechanism of action of disulfiram on the respiratory electron transport system of the liver mitochondria was studied in vitro. 2. Disulfiram inhibited the respiration supported by malate-glutamate as well as succinate. 3. Mitochondrial respiration inhibition was dependent upon alteration of -SH groups. 4. The inhibitory action of disulfiram might be related to the crosslinking of several proteins of the inner mitochondrial membrane. 5. The effects described above could be attributed to disulfiram per se and not to the main metabolite diethyldithiocarbamate.
Kuroda
M A
MA
Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, México, D.F.
Cuéllar
A
A
D003573:Cytochrome b Group / Q000187:drug effects; D004221:Disulfiram / Q000494:pharmacology*; D004050:Ditiocarb / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004579:Electron Transport / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D066298:In Vitro Techniques; D008293:Malates / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid
D003573:Cytochrome b Group; D005971:Glutamates; D008293:Malates; D013386:Succinates; D018698:Glutamic Acid; C030298:malic acid; D004050:Ditiocarb; D019802:Succinic Acid; D004221:Disulfiram