Expression of trkC in a mouse osteoblastic cell line and its response to neurotrophin-3.
In a cultured osteoblastic cell line, MC3T3-E1 derived from newborn mouse calvaria, the mRNA encoding TRKC, which is the receptor molecule of neurotrophin-3 (NT-3), was detected by the polymerase chain reaction (PCR) method. The mRNAs of the normal type and one alternative form (C14) were highly expressed in the exponential growth phase of MC3T3-E1 cells and decreased as the cells reached the differentiation stage. NT-3, but not nerve growth factor (NGF), stimulated the proliferation of MC3T3-E1 cells in a dose-dependent manner. NT-3 also stimulated calcium incorporation through the surface of MC3T3-E1 cells, indicating the association of NT-3 and its receptor on the cell surface.
Nakanishi
T
T
Department of Biochemistry and Molecular Dentistry, Okayama University Dental School, Japan.
Ohyama
K
K
Aoki
C
C
Kudo
A
A
Hattori
T
T
Takahashi
K
K
Taniguchi
S
S
Takigawa
M
M
D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology*; D020933:Neurotrophin 3; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D020812:Receptor, trkC; D017475:Receptors, Nerve Growth Factor / Q000235:genetics*
D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D020812:Receptor, trkC; D002118:Calcium