Bilateral retinal responses during the acute phase (4-14 days) after traumatization of a single eye in the mouse.
The infiltration of T lymphocytes and macrophages was examined in the retinae of traumatized and non-traumatized contra-lateral eyes of mice. Traumatization was produced by channeling a small hole in the limbic region of a single eye in each animal while it was under Nembutal anesthesia and a small piece of iron wire was inserted into the eye. After the T lymphocytes and macrophages were made apparent by immuno-histochemistry, they were counted on sectioned strips of the peripheral retinae 250 micrometer in length in both traumatized and non-traumatized eyes. During the first two weeks after the induced disturbance, both the retinae of the traumatized and non-traumatized eyes showed infiltration of T lymphocytes and macrophages. Although this increase in T lymphocyte infiltration was observed in both damaged eye and the undamaged one, the former displayed an enhanced increase. The contrary was noticed for macrophages infiltrating the undamaged eye. A bilateral response was therefore evident through the cellular infiltration in the traumatized and un-traumatized eyes. Morphometrical observations also revealed nuclear size changes in the retina during this acute phase of traumatization.
Li
W W
WW
Department of Anatomy, Chinese University of Hong Kong, Shatin.
Shen
W Z
WZ
Hung
Y
Y
Jen
P Y
PY
Yew
D T
DT
D000208:Acute Disease; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D015807:Eye Injuries, Penetrating / Q000473:pathology*; D005549:Foreign-Body Reaction / Q000473:pathology; D007249:Inflammation; D008264:Macrophages / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009879:Ophthalmia, Sympathetic / Q000473:pathology*; D012160:Retina / Q000473:pathology*; D012165:Retinal Ganglion Cells / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000473:pathology*; D014822:Vitreous Body / Q000473:pathology