Detection of P-glycoprotein in squamous cell carcinomas of the head and neck.
One established mechanism of multidrug resistance is elevated expression of P-glycoprotein. The expression of P-glycoprotein by immunohistochemistry was examined in squamous cell carcinomas of the head and neck using a newly developed monoclonal antibody, UIC2, which is specific to the human MDR1 gene product and recognizes an external epitope of the protein. P-glycoprotein was detected in 60% of the samples. MDR1 expression was compared with clinical response to chemotherapy in eight patients who received MDR1-dependent drugs and response was accurately predicted in seven (89%) of eight patients. Positive P-glycoprotein staining correlated with the degree of tumor differentiation, but not with other clinical factors. Therefore, analyzing the expression of P-glycoprotein may play a role when planning chemotherapeutic regimens for patients with head and neck cancer and may be an additional prognostic and diagnostic tool in these patients.
Kelley
D J
DJ
Department of Otolaryngology-Head and Neck Surgery, College of Medicine, University of Cincinnati, Ohio 45267-0528.
Pavelic
Z P
ZP
Gapany
M
M
Stambrook
P
P
Pavelic
L
L
Gapany
S
S
Gluckman
J L
JL
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000453:epidemiology / Q000473:pathology*; D002454:Cell Differentiation; D004351:Drug Resistance; D005069:Evaluation Studies as Topic; D015870:Gene Expression; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000453:epidemiology / Q000473:pathology*; D006785:Hospitals, University; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards; D008207:Lymphatic Metastasis; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics; D009367:Neoplasm Staging; D009820:Ohio / Q000453:epidemiology; D010641:Phenotype; D011237:Predictive Value of Tests; D011379:Prognosis; D015996:Survival Rate
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins