Purification of the Escherichia coli integration host factor (IHF) in one chromatographic step.
The integration host factor (IHF) participates in a diverse array of DNA transactions such as replication, recombination and gene expression. We describe a fast and very efficient isolation procedure which yields highly purified IHF in one chromatographic step.
Filutowicz
M
M
Department of Bacteriology, University of Wisconsin, Madison 53706.
Grimek
H
H
Appelt
K
K
D001426:Bacterial Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry*; D034982:Integration Host Factors
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D034982:Integration Host Factors