Expression of tau exon 8 in different species.
A synthetic peptide corresponding to a region encoded by tau gene exon 8 was used to raise an antibody. The antibody, E8, was used to probe normal tau from different species and abnormal tau proteins from subjects with Alzheimer's disease. Immunoblotting and enzyme-linked immunosorbent assays demonstrated that only bovine tau reacted with the E8 antibody. The E8 immunoreactive tau isoform was estimated to represent less than 2% of the bovine tau. Our results indicate that bovine tau is unique in containing isoforms positive with E8, and that PHF formation does not require the presence of PHF-tau with exon 8.
Chen
W T
WT
Department of Physiology, National Yang-Ming Medical College, Taipei, Taiwan.
Liu
W K
WK
Yen
S H
SH
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005091:Exons / Q000502:physiology*; D005260:Female; D006801:Humans; D007536:Isomerism; D008969:Molecular Sequence Data; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D016875:tau Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D016875:tau Proteins