Antimitotic action of extracts of Petiveria alliacea on sea urchin egg development.
The hydroethanol extract of the roots of Petiveria alliacea L. (Phytolaccaceae) has been investigated previously as an antitumor agent against mouse Ehrlich ascites. The extract and its methanol, butanol and ether fractions exhibited an antimitotic effect on sea urchin egg development. The aqueous fraction did not produce inhibition of cell cleavage. At the first cleavage the inhibition, at the lowest concentration (10 micrograms/ml), produced by the ether fraction was 42%, whereas the inhibition produced by the total extract and by the other fractions was only 5 to 10% showing that the ether fraction was the most active. Even at higher concentrations the butanol and methanol fractions inhibit the cleavage about 30%. At the first cleavage, the ED50 of the hydroethanol extract and of the ether fraction were 45.02 and 12.40 micrograms/ml, respectively. Furthermore, in the second cleavage, the hydroethanol extract was about twice as potent as the methanol or butanol fractions (ED50 of 22.40, 44.80 and 54.10 micrograms/ml, respectively).
Malpezzi
E L
EL
Departamento de Fisiologia Geral, Universidade de São Paulo, Brasil.
Davino
S C
SC
Costa
L V
LV
Freitas
J C
JC
Giesbrecht
A M
AM
Roque
N F
NF
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects*; D010063:Ovum / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology*; D012617:Sea Urchins
D010936:Plant Extracts