Targeting of an enhancer trap to vestigial.
The vestigial gene (vg+) is required for normal wing development and is expressed in a spatially distinct pattern in imaginal discs. We have exploited a general property of P element alleles to target an enhancer trap to the 5' region of the gene. By replacing the P element resident at this site in vg21 with a P element carrying a lacZ reporter gene, the vglacZ1 allele was selected on the basis of its increased mutant phenotype. In contrast to vg+ expression, which occurs primarily in the presumptive wing margin and hinge, beta-galactosidase expression in vglacZ1 wing discs is localized to the dorsal wing surface and displays homologous haltere expression. The targeting of P element enhancer traps could be readily extended to other genes with low rates of primary P element insertion.
Staveley
B E
BE
Department of Genetics, University of Alberta, Edmonton, Canada.
Hodgetts
R B
RB
O'Keefe
S L
SL
Bell
J B
JB
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D004251:DNA Transposable Elements; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D007814:Larva; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004251:DNA Transposable Elements; D004247:DNA