CGRP is expressed in primary cultures of human hepatocytes and in normal liver.
We recently reported that human liver and primary cultures of hepatocytes express calcitonin. We therefore studied the expression of calcitonin gene related peptide (CGRP), the alternative splicing product of the calcitonin gene, in hepatocytes and liver. We used polymerase chain reaction amplification with specific primers to detect the presence of CGRP I and II messengers and a specific radioimmunoassay to measure the peptide. We report here that CGRP is synthesized by primary cultures of hepatocytes and in liver. As liver also possesses specific receptors for CGRP in non-parenchymal cells, a paracrine system could be involved in liver metabolism.
Bracq
S
S
INSERM U349, Paris, France.
Clement
B
B
Pidoux
E
E
Moukhtar
M S
MS
Jullienne
A
A
D017398:Alternative Splicing; D001483:Base Sequence; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002478:Cells, Cultured; D017931:DNA Primers; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011863:Radioimmunoassay
D017931:DNA Primers; D015740:Calcitonin Gene-Related Peptide