Mutation of the p53 gene in gallbladder cancer.
Genetic alteration of the p53 gene was examined in tissue specimens of primary gallbladder cancer (GBC) using PCR-single strand conformation polymorphism (SSCP) and direct sequencing analyses. The p53 gene mutation was detected in five of the 16 GBC cases examined (31%). All five cases showed single point mutations; four were mutations resulting in amino acid substitution and the other was one base-pair deletion. Of the five mutations detected, four occurred at evolutionarily conserved regions and the remaining one at a well conserved region among species. Our result is similar to those of previous reports for various human cancers in mutation scattering over four of the five conserved domains.
Takagi
S
S
Department of Hygiene and Preventive Medicine, Niigata University School of Medicine.
Naito
E
E
Yamanouchi
H
H
Ohtsuka
H
H
Kominami
R
R
Yamamoto
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D004252:DNA Mutational Analysis; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005706:Gallbladder Neoplasms / Q000209:etiology / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004273:DNA, Neoplasm