Integrative transformation of the mycotoxin-producing fungus, Penicillium paxilli.
A high frequency transformation system has been developed for Penicillium paxilli using pAN7-1. Up to 44% of the primary transformants were heterokaryons. Loss of hygromycin resistance was observed in primary transformants that were sub-cultured on non-selective media, but single spores of these primary transformants were mitotically stable on both selective and non-selective media. A molecular analysis of the transformants generated showed that 78% had single-site integrations, with half of these containing a single copy of pAN7-1. CHEF-gel electrophoresis showed that P. paxilli has at least six chromosomes with a total genome size of about 23.4 Mb.
Itoh
Y
Y
Department of Plant Pathology, Tottori University, Japan.
Johnson
R
R
Scott
B
B
D015825:Chromosomes, Fungal; D004352:Drug Resistance, Microbial / Q000235:genetics; D006921:Hygromycin B / Q000494:pharmacology; D007211:Indoles / Q000378:metabolism*; D010407:Penicillium / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014170:Transformation, Genetic
D007211:Indoles; D011993:Recombinant Fusion Proteins; C048220:paxilline; D006921:Hygromycin B; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase