title: An agar-overlay method for detection of toxins produced by Escherichia coli.
abstract: An agar overlay method, with Vero and HeLa cells, was used for detection of heat-labile enterotoxin and verotoxin from Escherichia coli. The method is more sensitive than the conventional cell culture assay, is rapid, easy to perform, and is suitable for epidemiological studies.
authors.affiliation: Departamento de Microbiologia e Imunologia, Universidade Estadual de Campinas, São Paulo, Brazil.
authors.forename: V R
authors.initials: VR
authors.lastname: Parreira
authors.forename: C W
authors.initials: CW
authors.lastname: Arns
authors.forename: T
authors.initials: T
authors.lastname: Yano
mesh_terms: D000362:Agar; D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis*; D001431:Bacteriological Techniques; D004768:Enterotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006367:HeLa Cells; D006801:Humans; D012680:Sensitivity and Specificity; D022622:Shiga Toxin 1; D014709:Vero Cells
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022622:Shiga Toxin 1; D000362:Agar; C027538:heat-labile enterotoxin, E coli