One-step enrichment of nucleated red blood cells. A potential application in perinatal diagnosis.
We describe a discontinuous triple density gradient to obtain a 25-fold enrichment of nucleated red blood cells from mononuclear cells, granulocytes, and mature red blood cells in a single centrifugation step.
Bhat
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Department of Gynecology and Obstetrics, Stanford University, CA 94305.
Bieber
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Teng
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D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; D018826:CD13 Antigens; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis* / Q000235:genetics; D017493:Leukocyte Common Antigens; D011247:Pregnancy / Q000097:blood; D011990:Receptors, Transferrin
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; C068126:CD71 antigen; D011990:Receptors, Transferrin; D017493:Leukocyte Common Antigens; D018826:CD13 Antigens