Inhibition of topoisomerase II by aurintricarboxylic acid: implications for mechanisms of apoptosis.
Internucleosomal fragmentation of DNA, the most widely used biochemical indicator of apoptosis, is believed to contribute to loss of viability because the nuclease inhibitor, aurintricarboxylic acid, delays or prevents cell death in a range of experimental systems. We report here that auritricarboxylic acid inhibits topoisomerase II in vitro, the concentration required (< or = 0.2 microM) being less than that usually employed in studies of apoptosis. Since topoisomerase II mediates chromatin condensation during apoptosis, the efficacy of ATA in preventing or delaying cell death may not be the result of nuclease inhibition.
Catchpoole
D R
DR
Children's Leukaemia and Cancer Research Centre, University of New South Wales, Prince of Wales Children's Hospital, Sydney, Australia.
Stewart
B W
BW
D017209:Apoptosis / Q000187:drug effects*; D001312:Aurintricarboxylic Acid / Q000494:pharmacology*; D006801:Humans; D059005:Topoisomerase II Inhibitors
D059005:Topoisomerase II Inhibitors; D001312:Aurintricarboxylic Acid