Determination of the antifilarial drug UMF-078 and its metabolites UMF-060 and flubendazole in whole blood using high-performance liquid chromatography.
A rapid and selective high-performance liquid chromatographic (HPLC) method for the simultaneous determination of the antifilarial drug UMF-078 (I) and its metabolites UMF-060 (II) and flubendazole (III) is described. After a simple extraction from whole blood, the compounds were determined by HPLC using a C18 Inertsil ODS-2 reversed-phase column with methanol-0.05M ammonium acetate (pH 4.0) as the mobile phase and ultraviolet detection at 291 nm. The average recoveries of I, II and III over the concentration range 20-500 ng ml-1 were 69.9 +/- 4.7, 85.6 +/- 4.4 and 85.1 +/- 6.0%, respectively. The minimum detectable concentrations in whole blood for I, II and III were 10, 7 and 7 ng ml-1, respectively. This method was found to be suitable for pharmacokinetic studies.
Ramanathan
S
S
Centre for Drug Research, Universiti Sains Malaysia, Penang.
Nair
N K
NK
Mansor
S M
SM
Navaratnam
V
V
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D005369:Filaricides / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D008252:Macaca fascicularis / Q000097:blood; D008463:Mebendazole / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
D005369:Filaricides; C089376:UMF 060; C089375:UMF 078; D008463:Mebendazole; C018945:flubendazole