Affinity-purified Escherichia coli J5 lipopolysaccharide-specific IgG protects neutropenic rats against gram-negative bacterial sepsis.
Antibodies were raised in rabbits by immunization with the heat-killed J5 mutant of Escherichia coli O111 (Rc chemotype). Serum antibodies were separated into purified IgG and IgM by sequential affinity chromatography on protein G-Sepharose and anti-rabbit IgG-Sepharose columns. J5 lipopolysaccharide (LPS)-specific IgG was prepared by affinity chromatography of purified IgG on a J5 LPS-EAH Sepharose 4B affinity column. Purified IgM, IgG, and J5 LPS-specific IgG protected neutropenic rats against lethal challenge with Pseudomonas aeruginosa 12:4:4 (Fisher Devlin immunotype 6). Nine of 16 rats treated with the IgM fraction were protected (P < .001). Thirteen of 20 rats treated with the purified IgG and 6 of 8 treated with J5 LPS-specific IgG were protected compared with none of 25 treated with IgG made from the preimmune serum of the same rabbit (P < .001). These results demonstrate that purified J5 LPS-specific IgG protects against the lethal consequences of gram-negative bacteremia.
Bhattacharjee
A K
AK
Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Opal
S M
SM
Palardy
J E
JE
Drabick
J J
JJ
Collins
H
H
Taylor
R
R
Cotton
A
A
Cross
A S
AS
D000818:Animals; D000918:Antibody Specificity; D016470:Bacteremia / Q000276:immunology / Q000517:prevention & control*; D001428:Bacterial Vaccines / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000494:pharmacology*; D008050:Lipid A / Q000276:immunology / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000276:immunology* / Q000302:isolation & purification; D009503:Neutropenia / Q000276:immunology*; D011552:Pseudomonas Infections / Q000276:immunology / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa; D011817:Rabbits / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley
D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D008050:Lipid A; D008070:Lipopolysaccharides