title: Apically localized IP3 receptors control chloride current in airway gland acinar cells.
abstract: We examined the role of inositol 1,4,5-trisphosphate (IP3) receptors in acetylcholine (ACh)-induced chloride (Cl-) current in acinar cells of human and feline airway submucosal glands, using whole cell patch-clamp analysis. ACh (10 nM-1 microM) induced an initial Cl- current followed by a K+ current, and lower doses of ACh (1-10 nM) often induced oscillations of both currents, which were mimicked by the application of intracellular IP3. Neither isoproterenol (-10 microM) nor raising intracellular adenosine 3,5-cyclic monophosphate induced any current. Caffeine (20-50 mM) and intracellular ryanodine (1-100 microM) induced a K+ current alone without Cl- current. Monoclonal antibodies to the IP3 receptor abolished both ACh-induced K+ and Cl- currents. Immunohistochemical analysis revealed the localization of IP3 receptors on both the cytosol and some regions of the endoplasmic reticulum beneath the apical membrane of acinar cells. These results indicate that apically localized IP3 receptors control Cl- secretion from airway submucosal gland cells.
authors.affiliation: Department of Medicine and Physiology, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: S
authors.initials: S
authors.lastname: Shimura
authors.forename: M
authors.initials: M
authors.lastname: Wakui
authors.forename: Y
authors.initials: Y
authors.lastname: Ohkawara
authors.forename: T
authors.initials: T
authors.lastname: Takishima
authors.forename: K
authors.initials: K
authors.lastname: Mikoshiba
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000276:immunology / Q000502:physiology*; D002415:Cats; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000502:physiology*; D004553:Electric Conductivity; D006801:Humans; D007150:Immunohistochemistry; D053496:Inositol 1,4,5-Trisphosphate Receptors; D015759:Ionomycin / Q000494:pharmacology; D015221:Potassium Channels / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear / Q000276:immunology / Q000502:physiology*; D014132:Trachea / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D015220:Calcium Channels; D002712:Chlorides; C506926:ITPR1 protein, human; D053496:Inositol 1,4,5-Trisphosphate Receptors; D015221:Potassium Channels; D018160:Receptors, Cytoplasmic and Nuclear; D015759:Ionomycin; D000109:Acetylcholine; D002118:Calcium