In vitro correlates of the L. casei animal model of Kawasaki disease.
The induction of coronary arteritis in mice by Lactobacillus casei cell wall (CW) is thought to represent an animal model of Kawasaki disease. Treatment of vascular endothelial cells (EC) in vitro with supernatants from CW stimulated human mononuclear cells (MNC) enhanced adherence of human polymorphonuclear leukocyte (PMN) to human EC, and EC expression of intercellular adhesion molecule-1 (ICAM-1) but not HLA-DR. Supernatants contained high concentrations of tumor necrosis factor-alpha (TNF-alpha) and PMN adherence correlated directly with the concentration of TNF-alpha. Intravenous human gamma globulin (IVGG) preparations did not block the effect of cytokine containing MNC supernates upon EC, ICAM-1 expression by EC, or PMN adherence to prestimulated EC. However, both EC ICAM-1 expression and enhanced PMN adherence to EC by CW induced MNC supernatants were blocked by anti-TNF-alpha treatment. The initial coronary inflammatory reaction in the mouse model appears to involve PMN adherence to vascular EC that have been activated by TNF-alpha released by MNC after stimulation with CW.
Tomita
S
S
Division of Infectious Diseases, Children's Memorial Hospital, Chicago, IL 60614.
Myones
B L
BL
Shulman
S T
ST
D000328:Adult; D000818:Animals; D001424:Bacterial Infections / Q000150:complications* / Q000382:microbiology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002473:Cell Wall / Q000382:microbiology / Q000502:physiology / Q000648:ultrastructure; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000382:microbiology / Q000502:physiology; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1; D007780:Lacticaseibacillus casei / Q000166:cytology / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000382:microbiology / Q000502:physiology; D051379:Mice; D009080:Mucocutaneous Lymph Node Syndrome / Q000209:etiology / Q000382:microbiology*; D009504:Neutrophils / Q000382:microbiology / Q000502:physiology
D015815:Cell Adhesion Molecules; D016756:Immunoglobulins, Intravenous; D018799:Intercellular Adhesion Molecule-1