Rapid biochemical screening for Salmonella, Shigella, Yersinia, and Aeromonas isolates from stool specimens.
Four screens for the rapid (4 to 6 h) biochemical detection of pathogens from enteric isolation media are described. The Salmonella screen consisted of Kligler iron agar (KIA), motility-indole-urea-tryptophan-deamination semisolid medium (MIU-TDA), and the o-nitrophenyl-beta-D-galactopyranoside (ONPG) test; the Shigella screen consisted of KIA, MIU-TDA, the ONPG test, and the lysine decarboxylation-indole test; the Yersinia screen consisted of a rhamnose broth; the Aeromonas screen consisted of a xylose agar plate. When tested on 2,102 fresh isolates and 71 stock strains, the screens correctly detected 212 enteric pathogens (sensitivity, 100%), with a specificity of 98.1%.
De Ryck
R
R
Laboratoire de Microbiologie, Hôpital Erasme, Université Libre de Bruxelles, Belgium.
Struelens
M J
MJ
Serruys
E
E
D000333:Aeromonas / Q000302:isolation & purification*; D000465:Algorithms; D003470:Culture Media; D004405:Dysentery, Bacillary / Q000175:diagnosis* / Q000382:microbiology; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D012475:Salmonella / Q000302:isolation & purification*; D012480:Salmonella Infections / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity; D012760:Shigella / Q000302:isolation & purification*; D013997:Time Factors; D015007:Yersinia / Q000302:isolation & purification*; D015009:Yersinia Infections / Q000175:diagnosis* / Q000382:microbiology
D003470:Culture Media