Effect of covalent attachment of polyethylene glycol on some properties of rat liver soluble alanine aminotransferase.
Soluble alanine aminotransferase from rat liver was modified with 2,4-bis(O-methoxypolyethylene glycol)-6-chloro-s-triazine which was synthesized from monomethoxypolyethylene glycol and cyanuric chloride. The modified enzyme exhibited relative stability against extreme conditions of pH and temperature and also resistivity against tryptic digestion as compared to the native enzyme.
Patnaik
S K
SK
Department of Pharmaceutics, Banaras Hindu University, Varanasi, India.
D000410:Alanine Transaminase / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D012995:Solubility; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014227:Triazines / Q000737:chemistry
D013438:Sulfhydryl Compounds; D014227:Triazines; D011092:Polyethylene Glycols; C019309:cyanuric chloride; C029761:2,4-bis(O-methoxypolyethylene glycol)-6-chloro-s-triazine; D000410:Alanine Transaminase