Some noradrenergic neurons of locus ceruleus-olfactory pathways contain neuropeptide-Y.
We report a specific tracing technique for studying projections of noradrenergic neurons that contain other transmitters. Autoradiography after retrograde axonal transport of tritiated-noradrenaline ([3H]NA) was combined with immunocytochemical detection of endogenous NA or neuropeptide Y (NPY). The specificity of [3H]NA retrograde labeling was dependent on the concentration of [3H]NA injected at the terminal region. At 2 x 10(-3) M, the specificity of [3H]NA retrograde labeling was confirmed by immunodetection of endogenous NA in radiolabeled cell bodies of the locus ceruleus. Combination of autoradiography of [3H]NA retrograde labeling with immunodetection of NPY demonstrated that a some scattered locus ceruleus noradrenergic neurons (about 26%) projecting to the olfactory bulb do contain NPY.
Bouna
S
S
Département de Cytologie, Université de Pierre et Marie Curie, Paris, France.
Gysling
K
K
Calas
A
A
Araneda
S
S
D000818:Animals; D001345:Autoradiography; D007150:Immunohistochemistry; D008125:Locus Coeruleus / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009478:Neuropeptide Y / Q000276:immunology / Q000378:metabolism*; D009638:Norepinephrine / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism; D009833:Olfactory Pathways / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D009478:Neuropeptide Y; D009638:Norepinephrine