Ability of a new infant formula prepared from partially hydrolyzed bovine whey to induce anaphylactic sensitization: evaluation in a guinea pig model.
In the present study we evaluated the allergenicity and immunogenicity of a whey partially hydrolysate formula (PHF) in a guinea pig model. Nine groups of 10 guinea pigs received either a conventional milk formula (CMF) or PHF for 37 days. After intravenous (i.v.) challenge with either beta-lactoglobulin (beta-L) or ultracentrifuged (u)CMF, animals fed CMF showed, respectively, 80% and 100% fatal reactions, whereas animals fed PHF showed no reactions when i.v. challenged both with beta-L and uPHF. Eighty percent fatal reactions, and 10%, respectively, of severe and mild reactions have been observed in the CMF-fed, casein-challenge group. In contrast, only one (10%) mild reaction occurred in the PHF-fed, casein-challenged group. When challenged with u-pasteurized cow's milk (uPCM), CMF-fed animals showed 86% fatal reactions and 14% mild reactions, whereas PHF-fed ones presented 70% no reactions, 20% mild reactions, and one (10%) fatal reaction. Animals fed CMF showed a significantly higher level of specific IgG against cow's milk antigens than PHF-fed ones, which, however, presented higher levels of antibodies than a water-supplemented control group. The present results confirm that PHF is less sensitizing than CMF in a guinea pig model.
Piacentini
G L
GL
Pediatric Department, University of Verona, Italy.
Bertolini
A
A
Spezia
E
E
Piscione
T
T
Boner
A L
AL
D000707:Anaphylaxis / Q000097:blood / Q000276:immunology*; D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002364:Caseins / Q000009:adverse effects / Q000276:immunology / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D007108:Immune Tolerance; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007782:Lactoglobulins / Q000009:adverse effects* / Q000276:immunology / Q000378:metabolism; D008892:Milk / Q000009:adverse effects* / Q000276:immunology / Q000378:metabolism; D016269:Milk Hypersensitivity / Q000097:blood / Q000276:immunology*; D008894:Milk Proteins / Q000009:adverse effects / Q000276:immunology / Q000378:metabolism; D008954:Models, Biological; D011492:Protein Hydrolysates / Q000009:adverse effects* / Q000276:immunology / Q000378:metabolism
D002364:Caseins; D007074:Immunoglobulin G; D007782:Lactoglobulins; D008894:Milk Proteins; D011492:Protein Hydrolysates