title: Immune response following oral administration of cholera toxin B subunit to HIV-1-infected UK and Kenyan subjects.
abstract: OBJECTIVE
  To determine the effect of HIV-1 infection on immunoglobulin (Ig) G and IgA antibody response and circulating antibody forming cell response to oral immunization with the B subunit of cholera toxin.
  
  
  METHODS
  Healthy UK volunteers, and HIV-1-positive UK and Kenyan volunteers at different clinical stages of HIV-1 infection received two oral immunizations. CD4+ T cells, serum beta 2-microglobulin and neopterin were measured as surrogate markers of disease stage, and correlated with immunization response.
  
  
  METHODS
  Serum antitoxin IgG and IgA measured by enzyme-linked immunosorbent assay and antitoxin IgG, IgA and IgM antibody-forming cells detected by enzyme-linked immunospot assay at different times after two oral immunizations.
  
  
  RESULTS
  UK HIV-positive volunteers (mean CD4+ T cell count, 52 x 10(6)/l) responded poorly to primary and booster immunization. HIV-infected Kenyans (752 x 10(6)/l CD4+ T cells) had a significant primary and booster antibody response, whereas those with a mean CD4+ T cell count 186 x 10(6)/l had an insignificant primary, but significant booster response. Two oral immunizations induced antibody responses in HIV-positive Kenyan groups (who may have prior immunity from exposure to environmental bacterial toxins) of similar or greater magnitude to healthy UK volunteers.
  
  
  CONCLUSIONS
  Mucosal immunization may recall immune memory and be of benefit in early and moderately advanced clinical HIV disease. The findings have important clinical implications in that mucosally targeted vaccines are potentially useful in this group of patients.
authors.affiliation: Division of Communicable Diseases, St George's Hospital Medical School, London, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Lewis
authors.forename: C F
authors.initials: CF
authors.lastname: Gilks
authors.forename: S
authors.initials: S
authors.lastname: Ojoo
authors.forename: L R
authors.initials: LR
authors.lastname: Castello-Branco
authors.forename: G
authors.initials: G
authors.lastname: Dougan
authors.forename: M R
authors.initials: MR
authors.lastname: Evans
authors.forename: S
authors.initials: S
authors.lastname: McDermott
authors.forename: G E
authors.initials: GE
authors.lastname: Griffin
mesh_terms: D000284:Administration, Oral; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology; D015415:Biomarkers; D002772:Cholera Toxin / Q000276:immunology*; D005260:Female; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D007630:Kenya; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology; D006113:United Kingdom
chemical_list: D015415:Biomarkers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D002772:Cholera Toxin