title: Microbiological tests to predict treatment outcome in experimental device-related infections due to Staphylococcus aureus.
abstract: Treatment outcome of experimental device-related infections cannot be predicted by the results of standard susceptibility tests such as MIC. Microorganisms involved in such infections have a slow growth rate and adhere to surfaces. Therefore, laboratory tests were developed taking into account these properties and compared with the treatment outcome in an animal model. Vancomycin, teicoplanin, ciprofloxacin and fleroxacin were tested alone, or in combination with rifampicin for their ability to cure experimental device-related infections in guinea pigs due to Staphylococcus aureus ATCC 29213. Rifampicin alone or in combination was significantly more effective than the other four drugs (P < 0.001). Combined treatment with rifampicin had a higher cure rate than rifampicin alone. Treatment success was not predicted by an antibiotic trough level exceeding the MIC at site of infection. In contrast, drug efficacy was predicted if the stationary-phase MBC was in the sensitive range, and if glass-adherent S. aureus was killed by low drug concentrations.
authors.affiliation: Department of Internal Medicine and Research, University Hospital, Basle, Switzerland.
authors.forename: W
authors.initials: W
authors.lastname: Zimmerli
authors.forename: R
authors.initials: R
authors.lastname: Frei
authors.forename: A F
authors.initials: AF
authors.lastname: Widmer
authors.forename: Z
authors.initials: Z
authors.lastname: Rajacic
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use; D001422:Bacterial Adhesion; D008297:Male; D008826:Microbial Sensitivity Tests; D011379:Prognosis; D016459:Prosthesis-Related Infections / Q000188:drug therapy* / Q000382:microbiology*; D051381:Rats; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology*; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents