Microwave-antigen retrieval for proliferation analysis (MIB-1) and quanticyt karyometry of bladder washings.
Bladder washings can be used for simultaneous karyometry and proliferation analysis using MiB-1 as a proliferation marker. We analyzed 42 problem cases with a discordance between cytologic and karyometric classification (QUANTICYT), in which the karyometric classification was based on a combination of a nuclear shape parameter and DNA (2cDI). Moreover, 25 concordant cases were analyzed: 5 normal samples, 6 low-grade tumors and 7 high-grade tumors. All normal samples and all low-grade tumors had labelling indices below 10%, and all high-grade tumors over 10%. For the discordant low-grade tumors, the QUANTICYT classification correlated better with the MiB-1 labelling than the cytologic diagnosis. There was a clear correlation between 2cDI and MiB-1 labelling index. Slightly elevated MiB-1 labelling indices might have some prognostic value.
Van der Poel
H G
HG
Department of Urology, University Hospital, Nijmegen, The Netherlands.
Boon
M E
ME
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000528:radiation effects; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000528:radiation effects; D002295:Carcinoma, Transitional Cell / Q000737:chemistry / Q000473:pathology*; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000032:analysis; D005500:Follow-Up Studies; D005629:Frozen Sections; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000528:radiation effects; D008872:Microwaves; D009363:Neoplasm Proteins / Q000032:analysis* / Q000528:radiation effects; D016612:Paraffin Embedding; D011489:Protein Denaturation / Q000528:radiation effects; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D007507:Therapeutic Irrigation; D001743:Urinary Bladder / Q000737:chemistry / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D008565:Membrane Proteins; D009363:Neoplasm Proteins