A method for the isolation of nuclear DNA from cotton (Gossypium) leaves.
Cotton leaves contain high levels of polyphenolic compounds that irreversibly interact with proteins and nucleic acids during DNA isolation. A procedure to isolate nuclear DNA from cotton (Gossypium hirsutum L.) has been developed. The method is based on the rapid initial isolation of nuclei in a glucose medium designed to stabilize nuclear structure and composition while preventing covalent interactions with polyphenolics. The resulting DNA is high in yield and purity and is suitable for Southern-blot hybridization analysis and restriction fragment length polymorphism analysis.
Dabo
S M
SM
Biochemistry and Molecular Biology Department, Oklahoma State University, Stillwater 74078-0454.
Mitchell
E D
ED
Melcher
U
U
D015139:Blotting, Southern; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000737:chemistry; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005069:Evaluation Studies as Topic; D005947:Glucose; D003368:Gossypium / Q000737:chemistry / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010944:Plants / Q000737:chemistry* / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length
D004247:DNA; D005947:Glucose