Human papillomavirus detection by hybrid capture and its possible clinical use.
OBJECTIVE
To determine the sensitivity of the hybrid capture method for human papillomavirus (HPV) detection and potential clinical uses as a screening method for the identification of cervical intraepithelial neoplasia.


METHODS
The presence of oncogenic types of HPV was tested for in samples taken from the cervix at colposcopy, and compared with detection by polymerase chain reaction (PCR) in 60 patients. Both sets of results were corrected with the pathology determined by biopsy and smear cytology.


RESULTS
Hybrid capture detection showed 86% agreement with PCR. Eighty three percent of CIN 3 lesions, 62% of CIN 2, 59% of CIN 1 and 21% of normal controls were positive for oncogenic HPV types.


CONCLUSIONS
The hybrid capture detection method is reliable, sensitive, and easy to use. The addition of HPV testing to cytological screening would detect a greater proportion of cervical dysplasia with a higher false positive rate.
Farthing
A
A
Ludwig Institute for Cancer Research, St Mary's Hospital, London.
Masterson
P
P
Mason
W P
WP
Vousden
K H
KH
D002584:Cervix Uteri / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D014773:Virology / Q000379:methods; D002578:Uterine Cervical Dysplasia / Q000821:virology*
D004279:DNA, Viral