Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew.
Taxomyces andreanae, a fungal endophyte, was isolated from the phloem (inner bark) of the Pacific yew, Taxus brevifolia. The fungus is hyphomyceteous and, when grown in a semi-synthetic liquid medium, produced taxol and related compounds. Taxol was identified by mass spectrometry, chromatography, and reactivity with monoclonal antibodies specific for taxol. Both [1-14C]acetic acid and L-[U-14C]phenylalanine served as precursors of [14C]taxol in fungal cultures. No taxol was detected in zero-time cultures or in the small agar plugs used to inoculate the culture flasks.
Stierle
A
A
Department of Plant Pathology, Montana State University, Bozeman 59717.
Strobel
G
G
Stierle
D
D
D000911:Antibodies, Monoclonal; D001643:Bridged Bicyclo Compounds / Q000378:metabolism*; D001952:Bridged-Ring Compounds; D002855:Chromatography, Thin Layer; D003470:Culture Media; D006801:Humans; D007124:Immunoenzyme Techniques; D007624:KB Cells; D003904:Mitosporic Fungi / Q000378:metabolism*; D017239:Paclitaxel / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D043823:Taxoids; D014197:Trees / Q000382:microbiology*
D000911:Antibodies, Monoclonal; D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D003470:Culture Media; D043823:Taxoids; C080625:taxane; D017239:Paclitaxel