Determination of ascorbic acid in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for precolumn deproteinization.
Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [detection limit, ca. 240 ng/ml plasma (at a signal-to-noise ratio of 2:1)], highly selective and reproducible [relative standard deviation, ca. 2.8% (n = 3)]. The calibration graph for AA was linear in the range 0.1-10 ng per injection (20 microliters). The recovery of AA was over 90% by the standard addition method.
Iwase
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Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Ono
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D001205:Ascorbic Acid / Q000097:blood*; D001798:Blood Proteins / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004355:Drug Stability; D017886:Durapatite; D004563:Electrochemistry; D006801:Humans; D008750:Methyldopa / Q000097:blood; D012680:Sensitivity and Specificity
D001798:Blood Proteins; D008750:Methyldopa; D017886:Durapatite; D001205:Ascorbic Acid