Time-dose response of Trypanosoma congolense bloodstream forms to diminazene and isometamidium.
Trypanosoma congolense bloodstream forms were propagated in vitro axenically in a simplified cultivation medium at 34 degrees C. Viability of a drug-sensitive and a drug-resistant clone were examined for 10 days following exposure to 0.1, 1.0 and 10.0 micrograms ml-1 of diminazene aceturate and 0.1, 1.0 and 10.0 ng ml-1 of isometamidium chloride for various time intervals. Drug-sensitive T. congolense were irreversibly damaged after incubation with 10 micrograms ml-1 or 1 microgram ml-1 diminazene aceturate for 30 min or 2 h, respectively, while drug-resistant trypanosomes were not affected. Exposure to 10 ng ml-1 isometamidium chloride eliminated drug-sensitive trypanosomes after 24 h and drug-resistant trypanosomes after 96 h. The data obtained on in vitro time-dose responses of T. congolense were related to pharmacokinetic data of diminazene and isometamidium in cattle plasma.
Kaminsky
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R
International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya.
Chuma
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F
Wasike
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RP
D000818:Animals; D004133:Diminazene / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D010617:Phenanthridines / Q000494:pharmacology*; D013997:Time Factors; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014348:Trypanosoma congolense / Q000187:drug effects*
D010617:Phenanthridines; D014344:Trypanocidal Agents; C000702:isometamidium chloride; C003915:diminazene aceturate; D004133:Diminazene