Structure and chromosomal locus of the mouse gene encoding a cerebellar Purkinje cell-specific helix-loop-helix factor Hes-3.
HES-3 is a cerebellar Purkinje cell-specific helix-loop-helix factor structurally related to the products of the Drosophila hairy and Enhancer of split genes. Here, we report the nucleotide sequence and chromosomal locus of the mouse Hes-3 gene. This gene consists of four exons and the exon-intron boundaries are well conserved when compared with those of the mouse Hes-1 and Drosophila hairy genes. Southern blot and interspecies backcross analyses show that the mouse Hes-3 gene is a single-copy gene and is located around position 80 on chromosome 4. Further analysis indicates that this locus is close to the Hes-5 locus, which is different from the Hes-1 locus (position 26 on chromosome 16). These results suggest that the Hes-3 and Hes-5 genes may be clustered on chromosome 4 while the Hes-1 gene is not.
Sakagami
T
T
Institute for Immunology, Kyoto University Faculty of Medicine, Japan.
Sakurada
K
K
Sakai
Y
Y
Watanabe
T
T
Nakanishi
S
S
Kageyama
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004247:DNA / Q000032:analysis / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D005260:Female; D005838:Genotype; D018257:Helix-Loop-Helix Motifs; D007438:Introns; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice / Q000235:genetics*; D016688:Mice, Inbred NOD / Q000235:genetics; D008815:Mice, Inbred Strains / Q000235:genetics; D008969:Molecular Sequence Data; D011689:Purkinje Cells / Q000378:metabolism*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D051792:Basic Helix-Loop-Helix Proteins; D004247:DNA; D004268:DNA-Binding Proteins; C083910:Hes3 protein, rat