title: In situ hybridization for localization of mRNAs in mononuclear phagocytes in cell culture and tissue sections.
abstract: We report an in situ hybridization procedure to detect in cell preparations and tissue sections messenger RNAs coding for mononuclear phagocyte proteins. The multistep procedure is described for use in conjunction with isotopic and non-isotopic probes.
authors.affiliation: Laboratoire d'Anatomie Pathologique, INSERM Unité 14, CHU, Nancy, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Vignaud
authors.forename: N
authors.initials: N
authors.lastname: Martinet
authors.forename: Y
authors.initials: Y
authors.lastname: Martinet
authors.forename: F
authors.initials: F
authors.lastname: Plenat
mesh_terms: D015342:DNA Probes; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes; D010586:Phagocytes / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D015342:DNA Probes; D015345:Oligonucleotide Probes; D015347:RNA Probes; D012333:RNA, Messenger