title: Multiple positive and negative cis-acting elements of the STA2 gene regulate glucoamylase synthesis in Saccharomyces cerevisiae.
abstract: Expression of the glucoamylase-encoding gene (STA2) in Saccharomyces cerevisiae was previously shown to be regulated transcriptionally by both positive and negative factors. The objective of this work was to identify the cis-acting elements responsible for STA2 transcriptional activation as well as the transcriptional repressor effects of STA10 and MATa/MAT alpha. We identified two upstream activation regions (UAS). Three repressor regions responsive to STA10-mediated repression were identified, as well as two regions for down-regulation of STA2 expression. MATa/MAT alpha repression appears to effect STA2 expression either downstream from the translational start site or, indirectly, since no functional a1/alpha 2-responsive sequence was identified in the promoter region.
authors.affiliation: Department of Microbiology, University of Stellenbosch, South Africa.
authors.forename: M G
authors.initials: MG
authors.lastname: Lambrechts
authors.forename: I S
authors.initials: IS
authors.lastname: Pretorius
authors.forename: V S
authors.initials: VS
authors.lastname: D'Aguanno
authors.forename: P
authors.initials: P
authors.lastname: Sollitti
authors.forename: J
authors.initials: J
authors.lastname: Marmur
mesh_terms: D001483:Base Sequence; D002244:Carbon / Q000378:metabolism; D015536:Down-Regulation; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics / Q000502:physiology*; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic / Q000235:genetics* / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
chemical_list: D002244:Carbon; D005087:Glucan 1,4-alpha-Glucosidase