Absence of functional inhibition of cloned human beta 2-adrenergic receptors by autoantibodies in asthmatic subjects.
Using guinea-pig lung membranes and cloned human beta 2-receptor adrenergic receptors the effects of whole serum, plasma, purified immunoglobulins and cellular activation products on beta 2-adrenergic receptor ligand binding and function were investigated. Sera from 24 non-asthmatic subjects and 115 asthmatics in different clinical categories were studied. There were no significant differences between antagonist ([125I] cyanopindolol) inhibition mediated by serum, plasma or by purified IgG when the asthmatics were compared with non-asthmatics. There was also no inhibition of 10(-6) M isoproterenol stimulated cAMP release from L cells expressing human beta 2-adrenergic receptors, by plasma, DEAE purified IgG fractions from asthmatics and non-asthmatics, or by products of activated platelets or lymphocytes. Since we have no evidence that immunoglobulins from asthmatic subjects exert functional inhibition of human beta 2-adrenergic receptors we conclude that autoantibodies to the beta 2-adrenergic receptors do not play an important functional role in the pathophysiology of asthma.
Potter
P C
PC
Department of Clinical Science and Immunology, University of Cape Town, Observatory, South Africa.
Van Wyk
L
L
White
D
D
Dakers
B S
BS
Chung
F Z
FZ
Dowdle
E B
EB
D000318:Adrenergic beta-Agonists / Q000627:therapeutic use; D000328:Adult; D000818:Animals; D001249:Asthma / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000502:physiology*; D002648:Child; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000096:biosynthesis; D006168:Guinea Pigs; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology; D020120:Iodocyanopindolol; D007545:Isoproterenol / Q000494:pharmacology; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000235:genetics / Q000276:immunology / Q000502:physiology*
D000318:Adrenergic beta-Agonists; D001323:Autoantibodies; D007074:Immunoglobulin G; D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D010869:Pindolol; D000242:Cyclic AMP; D007545:Isoproterenol