title: Paternity testing with VNTR DNA systems. II. Evaluation of 271 cases of disputed paternity with the VNTR systems D2S44, D5S43, D7S21, D7S22, and D12S11.
abstract: Paternity testing was carried out in 271 cases of disputed paternity using the 5 VNTR systems D2S44 (YNH24), D5S43 (MS8), D7S21 (MS31), D7S22 (g3), and D12S11 (MS43a), and 10-15 conventional marker systems including the HLA-A,B system. By means of the matching criteria for the VNTR systems established elsewhere (Morling & Hansen 1992), all 70 unrelated men who had been excluded by conventional typing were also excluded with 2 or more VNTR systems. Based on the observed exclusion frequencies for the 5 VNTR systems, a theoretical exclusion rate exceeding 0.999 could be obtained. A total of 350 father/child pairs were studied and in 3 paternity cases and one immigrant family, the alleged fathers were excluded solely by one of the 5 VNTR systems possibly reflecting mutations. No mother/child exclusions were observed among 350 mother/child pairs. Linkage analysis between the syntenic systems D7S21 (MS31) and D7S22 (g3) was performed in 29 informative families with 81 children and revealed a recombination distance of about 31 cM. The positive evidence for paternity provided by the 5 VNTR systems in cases with non-exclusions is discussed.
authors.affiliation: Institute of Forensic Genetics, University of Copenhagen, Denmark.
authors.forename: H E
authors.initials: HE
authors.lastname: Hansen
authors.forename: N
authors.initials: N
authors.lastname: Morling
mesh_terms: D000328:Adult; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D003718:Denmark; D005260:Female; D005819:Genetic Markers / Q000235:genetics*; D005828:Genetics, Population; D006801:Humans; D007223:Infant; D008297:Male; D010334:Paternity; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA