A novel dodecadepsipeptide, cereulide, isolated from Bacillus cereus causes vacuole formation in HEp-2 cells.
A HEp-2 cell-vacuolation factor was extracted and purified from the culture supernatant of a Bacillus cereus strain which caused emetic-syndrome food poisoning. The final preparation was chemically pure, and the toxin was named as cereulide. Mass spectrometry, NMR studies and chemical degradation revealed that the cereulide is a cyclic dodecadepsipeptide, (D-O-Leu-D-Ala-L-O-Val-L-Val)3, which is closely related to the potassium ionophore, valinomycin.
Agata
N
N
Nagoya City Public Health Research Institute, Japan.
Mori
M
M
Ohta
M
M
Suwan
S
S
Ohtani
I
I
Isobe
M
M
D000595:Amino Acid Sequence; D001409:Bacillus cereus / Q000737:chemistry* / Q000472:pathogenicity; D001427:Bacterial Toxins / Q000737:chemistry / Q000633:toxicity*; D002478:Cells, Cultured / Q000473:pathology; D047630:Depsipeptides; D005517:Foodborne Diseases / Q000209:etiology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry / Q000633:toxicity*; D014617:Vacuoles; D014774:Virulence
D001427:Bacterial Toxins; D047630:Depsipeptides; D010456:Peptides, Cyclic; C089294:cereulide