Standardized multicentric quantimetry of differentiation antigens expression. The GEIL's approach in acute lymphoblastic leukemia. Groupe d'Etude Immunologique des Leucémies.
The use of flow cytometry as a common tool has opened the field of quantimetric immunophenotyping. This could help refine the definition of leukemic proliferations, and perhaps provide new prognostic criteria. We investigated whether this approach could be applied to multicentric immunophenotyping. The reproducibility of quantitative measurements on different types of flow cytometers was tested using a single batch of calibrating and "blind" fluorescent beads. Less than 7% CV was obtained between 20 different laboratories. We then explored the variations noted with control or normal lymphocytes, using various CD4 monoclonal antibodies, which were found to be lower than 10%. We finally applied this strategy to acute lymphoblastic leukemias (ALL), performing quantitative measurements of CD10 using one single aliquoted batch of FITC-CD10 in comparison with locally used reagents. PreB-ALL could indeed be further stratified depending on the density of CD10 expression. Use of the same monoclonal antibody is however recommended, since PreB-ALL also seem to display epitopic variations of the CD10 molecule.
Philip
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Laboratoire d'Immunologie, Faculté de Médecine de Nancy, Vandoeuvre les Nancy, France.
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D015704:CD4 Antigens / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D015260:Neprilysin / Q000032:analysis*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology*; D015203:Reproducibility of Results
D015704:CD4 Antigens; D015260:Neprilysin