Preparation and characterization of monoclonal antibodies against equine chondrocytes, osteoblasts and osteocytes.
Three monoclonal antibodies capable of individually recognizing chondrocytes, osteoblasts and osteocytes were prepared. EB-1 reacted with a 55-kDa antigen on the chondrocyte membrane, EB-2 with a 110-kDa antigen on the membrane of osteoblasts and/or partial osteocytes, and EB-3 with a 130-kDa antigen on the membrane of osteocytes. These monoclonal antibodies may be useful probes for studying the differentiation and maturation of osteogenic cells.
Katayama
Y
Y
Pathology Division, Equine Research Institute, Japan Racing Association, Tokyo.
Oikawa
M
M
Kaneko
M
M
Yoshihara
T
T
Yoshikawa
H
H
Yoshikawa
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002356:Cartilage / Q000166:cytology / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D006825:Hybridomas; D051379:Mice; D010006:Osteoblasts / Q000276:immunology*; D010011:Osteocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal