title: Nucleotide sequence and genome organization of peanut stripe potyvirus.
abstract: A blotch isolate of peanut stripe virus (PStV) was cloned, sequenced and compared with other full-length potyvirus sequences. The viral genome was 10059 nucleotides (nt) in length excluding the poly(A) tail. Two potential AUG start codons were identified in the 5' non-translated region. Analysis of in vitro translation products from transcripts containing the first 600 nt of the PStV genome indicated that the first AUG (nt 134 to 136) was preferred over the second AUG (nt 146 to 148) for initiation of translation. Within the single large open reading frame, eight processed proteins were predicted. In general, motifs conserved in other potyviral sequences were also found in the PStV genome. The presence of a 6K protein between the P3 and CI proteins was predicted. An altered amino acid motif, from FI(V)VRG to FMIIRG, within the carboxyl terminus of the P1 protein, separates the PStV sequence from the majority of potyvirus sequences. Based on comparisons with available full-length potyvirus genome sequences, PStV was found to be most closely related to soybean mosaic virus.
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73402.
authors.forename: U B
authors.initials: UB
authors.lastname: Gunasinghe
authors.forename: S
authors.initials: S
authors.lastname: Flasinski
authors.forename: R S
authors.initials: RS
authors.lastname: Nelson
authors.forename: B G
authors.initials: BG
authors.lastname: Cassidy
mesh_terms: D000595:Amino Acid Sequence; D010367:Arachis / Q000382:microbiology; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D017124:Conserved Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017800:Potyvirus / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D003062:Codon; D014764:Viral Proteins