IL-8 production by peripheral blood mononuclear cells in nephrotic patients.
We studied the interleukin 8 (IL-8) gene expression by peripheral blood mononuclear cells (PBMC) and the IL-8 serum concentration in patients with idiopathic minimal lesion nephrotic syndrome (IMLNS) and other glomerulopathies. PBMC from eight of the nine (IMLNS) patients in relapse demonstrated the presence of IL-8 mRNA. All three IMLNS patients in remission (P = 0.0026 when compared to patients in relapse) and the two patients with nephrotic syndrome with other glomerulopathies failed to elicit an IL-8 mRNA response. Eleven of the 12 IMLNS patients in relapse showed IL-8 serum concentration above the level of detection. Only one of the seven patients in remission had detectable serum levels of IL-8 (P = 0.0033 when compared to levels from IMLNS patients in relapse). IL-8 serum levels were not detectable in three patients with nephrotic syndrome and other glomerulopathies. Supernatants of PBMC cultures from IMLNS patients in relapse increased the 35sulfate uptake by rat GBM. This effect was abolished by the addition of anti-IL-8 neutralizing antibody to the culture media and reproduced by the addition to the media of IL-8 in concentrations found in the serum of IMLNS patients in relapse. Finally, the effect of IL-8 on the 35sulfate turnover of the glomerular basement membrane (GBM) sulfated compounds was evaluated in vitro. A significant decrease in the percentage of residual 35sulfate incorporated in the GBM (41 +/- 5, mean +/- SEM) was observed in cultures treated with IL-8 as compared to those that were not treated with IL-8 (58 +/- 8, P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Garin
E H
EH
Department of Pediatrics, University of South Florida, Tampa.
Blanchard
D K
DK
Matsushima
K
K
Djeu
J Y
JY
D000293:Adolescent; D000328:Adult; D000818:Animals; D001485:Basement Membrane / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D005921:Glomerulonephritis / Q000097:blood / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000097:blood / Q000494:pharmacology; D007678:Kidney Glomerulus / Q000187:drug effects; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D009402:Nephrosis, Lipoid / Q000097:blood / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D012008:Recurrence; D013431:Sulfates / Q000378:metabolism
D016209:Interleukin-8; D012333:RNA, Messenger; D011994:Recombinant Proteins; D013431:Sulfates