Function of isolated rabbit hearts perfused with erythrocyte suspension is not stable but improvement may be feasible with hemoglobin solution.
In the present study isolated rabbit hearts were perfused with erythrocyte suspensions (hematocrit 21.5 +/- 0.5%) or hemoglobin solutions according to Langendorff with a constant flow at 37 degrees C. In preliminary experiments three types of stroma-free hemoglobin were used: unmodified, but carefully purified, stroma-free hemoglobin (SFHb), HbNFPLP which is a chemically modified Hb molecule and polyHbNFPLP which is a polymer of HbNFPLP. In hearts perfused with erythrocyte suspensions left ventricular developed pressure and oxygen consumption decreased and perfusion pressure increased steadily from the beginning of the perfusion. Dark spots appeared on the surfaces of these hearts, which were the result of extravasation of erythrocytes. As a consequence capillaries probably became obstructed, leading to reduced cardiac function. Hearts perfused with stroma-free hemoglobin solutions showed an initial increase in left ventricular developed pressure after switching from Tyrode perfusion to perfusion with hemoglobin solutions. Left ventricular developed pressure and perfusion pressure were stable for about 2 hours in hearts perfused with SFHb and were reasonable for 2 hours when the heart was perfused with HbNFPLP or more than 4 hours with polyHbNFPLP. More extensive experiments with stroma-free hemoglobin solutions when these become available in sufficient quantities have, according to the results from preliminary experiments, the potential of showing good oxygen supply resulting in reasonable cardiac function.
Hak
J B
JB
Laboratory for Physiology, Free University, Amsterdam, The Netherlands.
Biessels
P T
PT
van Beek
J H
JH
Bakker
J C
JC
Westerhof
N
N
D000818:Animals; D001794:Blood Pressure; D004912:Erythrocytes / Q000378:metabolism*; D006321:Heart / Q000502:physiology*; D006454:Hemoglobins / Q000378:metabolism*; D066298:In Vitro Techniques; D007552:Isotonic Solutions; D009206:Myocardium / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption; D010477:Perfusion; D010952:Plasma Substitutes / Q000378:metabolism*; D011817:Rabbits
D006454:Hemoglobins; D007552:Isotonic Solutions; D010952:Plasma Substitutes; C037692:Tyrode's solution; C016484:hemoglobin, stroma free; D010100:Oxygen