title: [Application of immunoassay for the detection of giardiasis in children and adults].
abstract: During the experiments it was used the immunoenzymatic ELISA test which detected the antigen Giardia 65 (GSA 65) in stool. We were tested 96 children of private school in the age of 7 to 14 and 83 persons aged 13 to 65. The samples of the stool firstly examined with standard method: direct smear in isotonic solution of NaCl in Lugol liquid and decantation. The investigated population of children with standard method shows the presence of the Giardia lamblia cyst in one of the samples (1.04%) and with the ELISA test in two samples (2.08%). In second group of the examined persons both methods the standard and ELISA test shows the occurrence of Giardia cyst in the samples (1.2%).
authors.affiliation: Katedra i Zakład Biologii i Parazytologii Slaskiej Akademii Medycznej, Katowicach.
authors.forename: A
authors.initials: A
authors.lastname: Deryło
authors.forename: H
authors.initials: H
authors.lastname: Sodowska
authors.forename: D
authors.initials: D
authors.lastname: Grygierczyk
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000953:Antigens, Protozoan; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D016829:Giardia lamblia / Q000302:isolation & purification*; D005873:Giardiasis / Q000469:parasitology*; D006801:Humans; D008875:Middle Aged; D010270:Parasite Egg Count
chemical_list: D000953:Antigens, Protozoan
vernacular_title: Zastosowanie testu immunoenzymatycznego ELISA do diagnostyki giardiazy u dzieci i osób dorosłych.