Effects of insulin on the release of neuropeptide Y, [Met5]enkephalin and catecholamines from dog adrenal medulla.
Insulin-induced hypoglycaemia is a well-known stimulating factor for the release of adrenaline from adrenal medulla. The present experiment investigates the co-release of catecholamines and neuropeptides (neuropeptide Y and [Met5]enkephalin) from the adrenal medulla in chloralose-anaesthetized dogs after intravenous administration of insulin (0.3 U/kg). The increases in noradrenaline, adrenaline and [Met5]enkephalin were 29.6-, 23.8-, and 27.9-fold basal values, respectively whereas the neuropeptide Y increase was only 1.6 times the baseline. These results show that adrenal [Met5]enkephalin and catecholamines exhibit the same pattern of co-release which is not the case for adrenal neuropeptide Y.
Damase-Michel
C
C
Laboratoire de Pharmacologie Médicale et Clinique, INSERM U 317, Faculté de Médecine, Toulouse, France.
Giraud
P
P
Portolan
G
G
Montastruc
J L
JL
Montastruc
P
P
Tran
M A
MA
D000313:Adrenal Medulla / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002395:Catecholamines / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004744:Enkephalin, Methionine / Q000378:metabolism*; D005260:Female; D007275:Injections, Intravenous; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism*; D011863:Radioimmunoassay
D002395:Catecholamines; D007328:Insulin; D009478:Neuropeptide Y; D004744:Enkephalin, Methionine