Functional versus structural changes in the pathophysiology of acute ischemic renal failure in aging rats.
The aim of this study was to gain further insight into the greater susceptibility to acute ischemic renal failure (ARF, 30 min of renal arteries clamping) of old rats (O, 18 months) as against young rats (Y, 3 months). All the rats ate a hypoproteic diet (14% of casein) to avoid age-related glomerulosclerosis in O. Basal renal dynamics was similar in O and Y (Groups CON). One day after ARF, the decrease in GFR was more severe in O than in Y (-82% and -57% vs. respective CON, P < 0.05), due to a greater rise of RVR in O (+258%) than in Y (+104%). The histological renal damage after ischemia was comparable in the two groups with ARF. Five days after ARF, the recovery of renal function was characterized by a slower rise of GFR in O than in Y. In two further groups, two different scavengers of oxygen-free radicals, dimethylthiourea (DMTU) and superoxide dismutase (SOD), were administered at the time of arterial occlusion. DMTU had protective effects in Y but not in O (delta GFR was -28% and -72%, respectively); in contrast, SOD was more effective in O (delta GFR = -58%) than in Y rats (delta GFR = -40%). To test the hypothesis that such a difference was related to the capacity of SOD to increase the levels of nitric oxide (NO), four more groups of Y and O rats were pretreated with L-arginine (ARG), precursor of NO, in tap water (1.5%). No difference in renal dynamics was detected in basal conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
Sabbatini
M
M
Department of Nephrology, 1st Faculty of Medicine, University of Naples, Italy.
Sansone
G
G
Uccello
F
F
De Nicola
L
L
Giliberti
A
A
Sepe
V
V
Margri
P
P
Conte
G
G
Andreucci
V E
VE
D058186:Acute Kidney Injury / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control; D000375:Aging / Q000502:physiology*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004044:Dietary Proteins / Q000008:administration & dosage; D005919:Glomerular Filtration Rate; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000494:pharmacology; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology
D004044:Dietary Proteins; D009569:Nitric Oxide; C038983:1,3-dimethylthiourea; D001120:Arginine; D013482:Superoxide Dismutase; D013890:Thiourea; D019331:NG-Nitroarginine Methyl Ester