title: Effects induced by BC 264, a selective agonist of CCK-B receptors, on morphine-dependent rats.
abstract: The aim of this study was to investigate the possible interaction between neuronal cholecystokinin (CCK) and opiate dependence. Rats were made dependent to morphine and the ability of cholecystokinin-octapeptide (CCK-8) and Tyr(SO3H)-gNle-mGly-Trp-(NMe)Nle-Asp-Phe-NH2 (BC 264), a selective agonist of CCK-B receptors, to induce signs of morphine withdrawal after ICV injection was tested. Behavioral responses were compared to those occurring during the naloxone-precipitated morphine withdrawal syndrome. In contrast to naloxone, CCK-8 (0.1, 1, and 10 micrograms, ICV) did not precipitate any sign of withdrawal. BC 264 (0.1, 1, and 10 micrograms, ICV) induced a strong hyperlocomotion and wet dog shakes in morphine-dependent rats, the latter effect also observed in nondependent animals. In rats receiving acute morphine, BC 264 induced an opposite effect (i.e., blockade of morphine-induced hyperactivity). Taken together, these results suggest that CCK plays only a minor role in the expression of morphine physical dependence.
authors.affiliation: Département de Pharmacochimie Moléculaire et Structurale, U 266 INSERM, URA D1500 CNRS, Université René Descartes, Faculté de Pharmacie, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Maldonado
authors.forename: O
authors.initials: O
authors.lastname: Valverde
authors.forename: M
authors.initials: M
authors.lastname: Derrien
authors.forename: P
authors.initials: P
authors.lastname: Tejedor-Real
authors.forename: B P
authors.initials: BP
authors.lastname: Roques
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D002766:Cholecystokinin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D007276:Injections, Intraventricular; D008297:Male; D008969:Molecular Sequence Data; D009021:Morphine Dependence / Q000523:psychology*; D009043:Motor Activity / Q000187:drug effects; D009270:Naloxone / Q000494:pharmacology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000187:drug effects*; D013375:Substance Withdrawal Syndrome / Q000523:psychology
chemical_list: C084553:BC 264; D010446:Peptide Fragments; D011949:Receptors, Cholecystokinin; D009270:Naloxone; D002766:Cholecystokinin