Evolutionarily conserved function of CD28 in alpha beta T cell activation.
The functional role of the chicken homologue of CD28 was studied. It is expressed on all thymocytes, and both V beta 1- and V beta 2-family expressing peripheral alpha beta T cells. Peripheral gamma delta T cells are CD28-negative. Monoclonal antibody against CD28 had a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MoAb against TCR. V beta 1 and V beta 2 expressing cells responded equally well to stimulation with anti-CD28 in combination with PMA. These responses were resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. These data indicate a striking conservation of the costimulatory function of CD28 and emphasize the importance of this costimulatory pathway.
Arstila
T P
TP
Department of Medical Microbiology, Turku University, Finland.
Vainio
O
O
Lassila
O
O
D000818:Animals; D016227:Benzoquinones; D005075:Biological Evolution; D018106:CD28 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D002645:Chickens; D016572:Cyclosporine / Q000494:pharmacology; D047029:Lactams, Macrocyclic; D008213:Lymphocyte Activation / Q000187:drug effects; D011809:Quinones / Q000494:pharmacology; D016693:Receptors, Antigen, T-Cell, alpha-beta; D017828:Rifabutin / Q000031:analogs & derivatives; D013601:T-Lymphocytes / Q000276:immunology*
D016227:Benzoquinones; D018106:CD28 Antigens; D047029:Lactams, Macrocyclic; D011809:Quinones; D016693:Receptors, Antigen, T-Cell, alpha-beta; D017828:Rifabutin; C020754:herbimycin; D016572:Cyclosporine