Changes in the frequency of splenic immunocompetent cells in rats exposed to carbon monoxide during gestation.
The aim of the present study was to evaluate whether prenatal exposure to relatively low concentrations of carbon monoxide (CO) may alter the frequency of splenic cells either in young (15-21 days) or in aged rats (18 months). Wistar female rats were exposed to 75 and 150 ppm of CO from day 0 to day 20 of pregnancy, respectively. The results show that prenatal exposure to 150 ppm of CO significantly decreases the number of leucocyte common antigen (LCA+) cells in 21 day old male rats, whereas other cellular populations, such as macrophages, Major Histocompatibility (MHC) II cells, T and B lymphocytes display only a trend towards a reduction without achieving statistical significance. The alterations in LCA+ cell frequency produced by gestational exposure to CO were reversible. These data further extend previous findings showing that rats prenatally exposed to moderate concentrations of CO exhibit subtle immunological changes in the absence of overt signs of toxicity.
Giustino
A
A
Institute of Pharmacology, School of Medicine, University of Bari, Italy.
Carratu
M R
MR
Siro Brigiani
G
G
De Salvia
M A
MA
Pellegrino
N M
NM
Steardo
L
L
Jirillo
E
E
Cuomo
V
V
D000375:Aging / Q000276:immunology; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001402:B-Lymphocytes / Q000187:drug effects; D002248:Carbon Monoxide / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007121:Immunocompetence / Q000187:drug effects*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects
D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D002248:Carbon Monoxide; D017493:Leukocyte Common Antigens