Modulation of the spc operon affects growth and protein secretion in Bacillus subtilis.
A proximal segment of B. subtilis secY gene was placed under the control of the inducible spac promoter/Lac repressor system. This fusion was integrated into the chromosomal spc operon of B. subtilis via Campbell-like reciprocal recombination. The growth of the resulting strain was strongly IPTG dependent. With staphylokinase and alpha-amylase as reporter proteins it was found, that the protein secretion capacity of this strain was correlated to the conditions of repression or induction of the chromosomal spac promoter.
Breitling
R
R
Friedrich-Schiller-Universität Jena, Biologisch-Pharmazeutische Fakultät, Institut für Molekularbiologie, Germany.
Schlott
B
B
Behnke
D
D
D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D002874:Chromosome Mapping; D015536:Down-Regulation / Q000502:physiology; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D017930:Genes, Reporter; D007544:Isopropyl Thiogalactoside; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism; D009876:Operon / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics; D000069816:SEC Translocation Channels; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D000069816:SEC Translocation Channels; C057841:SecY protein, E coli; D007544:Isopropyl Thiogalactoside; D000516:alpha-Amylases; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus