Nucleotide sequence and characterization of the feline herpesvirus type 1 immediate early gene.
We report the nucleotide sequence of feline herpesvirus type 1 (FHV-1) immediate early (IE) gene encoding a homologue to the ICP4 protein of herpes simplex virus type 1 and its flanking sequences. When we examined the regulatory function of the FHV-1 IE gene product by using the transient transfection assay with an effector plasmid expressing the FHV-1 IE gene in combination with chimeric various promoter-chloramphenicol acetyltransferase reporter constructs, the product was able to transactivate a putative FHV-1 TK promoter, pseudorabies virus and equine herpesvirus type 1 ICP4 promoters, and human immunodeficiency virus (HIV) long terminal repeat (LTR) in Crandell feline kidney cells. Furthermore, the FHV-1 IE gene product could transactivate both feline immunodeficiency virus and HIV LTRs in SW480 cells but not in Felis catus whole fetus 4 cells.
Kawaguchi
Y
Y
Department of Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Maeda
K
K
Miyazawa
T
T
Ono
M
M
Kai
C
C
Mikami
T
T
D018138:Alphaherpesvirinae / Q000235:genetics*; D000818:Animals; D002415:Cats; D002478:Cells, Cultured; D015870:Gene Expression; D017781:Genes, Immediate-Early / Q000235:genetics*; D016325:HIV Long Terminal Repeat / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000502:physiology; D016089:Immunodeficiency Virus, Feline / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic
D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C048925:herpes simplex virus, type 1 protein ICP4