title: Multicenter evaluation of ATB fungus: a standardized micromethod for yeast susceptibility testing.
abstract: The micromethod for yeast susceptibility testing, ATB Fungus, was evaluated with 30 reference strains in three laboratories. Ready-to-use strips with 5-fluorocytosine, amphotericin B, nystatin, miconazole, econazole and ketoconazole were used. The test allowed the categorization of each strain as susceptible, intermediate or resistance to all the antifungals tested, and 5-fluorocytosine and amphotericin B MIC determination. The results were compared with the MIC for each reference strain obtained by a microdilution method on RPMI 1640 buffered with MOPS. The repeatability and intralaboratory and interlaboratory reproducibility were evaluated. ATB Fungus was a reliable and reproducible method with a repeatability of 96.6%, a reproducibility of 95.4% and showed an excellent correlation 91.7%) with reference MICs.
authors.affiliation: Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina, Universidad del País Vasco, Bilbao, España.
authors.forename: G
authors.initials: G
authors.lastname: Quindós
authors.forename: R
authors.initials: R
authors.lastname: Salesa
authors.forename: A J
authors.initials: AJ
authors.lastname: Carrillo-Muñoz
authors.forename: V
authors.initials: V
authors.lastname: Lipperheide
authors.forename: L
authors.initials: L
authors.lastname: Jáudenes
authors.forename: R
authors.initials: R
authors.lastname: San Millán
authors.forename: J M
authors.initials: JM
authors.lastname: Torres-Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: Pontón
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013045:Species Specificity; D015003:Yeasts / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents; D011933:Reagent Kits, Diagnostic