Pili of vibrio cholerae widely distributed in serogroup O1 strains.
The distribution of Vibrio cholerae O1 pili consisting of 16 kDa subunit protein (16K-pili) was examined by Western blotting, using 211 strains from various origins and specific anti-16K-pili sera. The 16 kDa protein was detected in all 211 strains. The pili were purified from 3 El Tor and 3 classical strains, and characterized by hemagglutination and inhibition tests. All purified pili were hemagglutinative. However, the hemagglutinating activity of classical pili disappeared after exposure to 5 M urea and the agglutination induced by the classical pili was inhibited by D-mannose, alpha-methylmannoside, D-glucose and N-acetylglucosamine. On the contrary, El Tor pili were resistant to these sugars and urea.
Iwanaga
M
M
Department of Bacteriology, University of the Ryukyus School of Medicine, Okinawa, Japan.
Nakasone
N
N
Yamashiro
T
T
Higa
N
N
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D022121:Cholera Vaccines / Q000302:isolation & purification; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D006384:Hemagglutination; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification / Q000276:immunology* / Q000648:ultrastructure
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D022121:Cholera Vaccines; D036781:Fimbriae Proteins