[The development of a rapid semiautomatic enzymatic analyzer of beta-lactam antibiotics].
A new method and a device for enzymatic express assay of beta-lactam antibiotics are described. The principle of the device operation is based on the use of native enzymes specific to the antibiotics assayed. The method makes it possible to exclude the influence of the buffer capacity of the samples on the results of the assay. The data on the testing of the method and device with model solutions of benzylpenicillin are presented. The relative error of a single measurement does not exceed 2 per cent. The time of an assay is not more than 3 minutes. Recommendations on the use of the device in research studies and manufacture are presented.
Itsygin
S B
SB
Levadnaia
T P
TP
Balanchivadze
A A
AA
Beriashvili
L D
LD
Karashvili
G D
GD
Bartoshevich
Iu E
IuE
D000900:Anti-Bacterial Agents / Q000032:analysis*; D001322:Autoanalysis / Q000295:instrumentation* / Q000379:methods; D002021:Buffers; D004563:Electrochemistry; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012996:Solutions; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D002021:Buffers; D012996:Solutions; D047090:beta-Lactams
Razrabotka fermentativnogo poluavtomaticheskogo ékspress-analizatora betalaktamnykh antibiotikov.