title: A PCR-based strategy for the rapid construction of defined bacterial deletion mutants.
authors.affiliation: St. Bartholomew's Hospital Medical College, London, UK.
authors.forename: B W
authors.initials: BW
authors.lastname: Wren
authors.forename: J
authors.initials: J
authors.lastname: Henderson
authors.forename: J M
authors.initials: JM
authors.lastname: Ketley
mesh_terms: D001419:Bacteria / Q000235:genetics*; D016123:Campylobacter jejuni / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D017353:Gene Deletion; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D014169:Transformation, Bacterial; D015008:Yersinia enterocolitica / Q000235:genetics