Formation, turnover, and sensitivity to phosphatidylinositol-specific phospholipase C of Thy-1 of a rat neuronal tumor cell line.
We have investigated the formation, turnover, and sensitivity to phosphatidylinositol-specific phospholipase C (PI-PLC) of Thy-1 in the rat neuronal tumor cell line BN-1010-1. It was initially established that a relatively short labeling time (1.5 h) using [3H]mannose yielded much more highly labeled PI-PLC-releasable glycoproteins than a longer labeling time (18 h). Labeled Thy-1 was released from the cell surface as early as 30 min postlabeling, increased to a maximum at 2.0 h, and then decreased to 50% of maximum by 5.5 h. The decrease may be due to degradation or spontaneous release into the medium, since total cellular Thy-1 remained constant during the decline. The decrease may be a result of a partial conversion of Thy-1 from a PI-PLC-sensitive to a PI-PLC-insensitive state. This resistance of the plasma membrane-associated Thy-1 was not due to a chemical modification of the glycoprotein, since detergent-extracted Thy-1 was completely sensitive to PI-PLC.
Wilkerson
L S
LS
Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235.
Touster
O
O
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D007700:Kinetics; D008358:Mannose / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D017830:Octoxynol; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011092:Polyethylene Glycols; D051381:Rats; D012995:Solubility; D018800:Thy-1 Antigens; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D005947:Glucose; D008358:Mannose