Cloning and characterization of a putative G-protein coupled receptor potentially involved in development.
Polymerase chain reaction techniques and medium stringency library screening were used to isolate a rat cDNA ("H218") which encodes a novel guanine nucleotide-binding protein coupled receptor homolog ("pH218"). Northern analysis revealed that brain H218 mRNA is preferentially expressed during embryogenesis. In addition, H218 mRNA is expressed in all developing tissues and rodent cell lines examined with highest levels detected in primitive, transformed cells. H218 mRNA expression in cell lines is rapidly increased by a tumor promoter and rapidly decreased by a differentiation-inducing growth factor. Finally, all of the sequence motifs characteristic of Src homology 2 domains are present in pH218 but in a unique arrangement. We conclude that pH218 may function as a growth factor receptor.
MacLennan
A J
AJ
Department of Neuroscience, University of Florida Brain Institute, University of Florida College of Medicine, Gainesville 32610.
Browe
C S
CS
Gaskin
A A
AA
Lado
D C
DC
Shaw
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D019204:GTP-Binding Proteins; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D016716:PC12 Cells / Q000187:drug effects; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D011954:Receptors, Dopamine / Q000235:genetics; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011954:Receptors, Dopamine; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D019204:GTP-Binding Proteins; D013755:Tetradecanoylphorbol Acetate