Efficiency of various strategies and materials to generate new markers: saturating the region 5q11.2-q13.3 with 30 new randomly distributed clones.
Several different strategies and materials were used for saturating the region 5q11.2-q13.3 with new, randomly distributed markers: isolation of human clones from three chromosome-5-specific libraries (a BssHII endclone phage library from the somatic cell hybrid H64 and two total genomic phage libraries from radiation hybrids IH12 and IH132), as well as Alu-PCR from chromosome-5-specific radiation hybrids with overlapping fragments in the region around the spinal muscular atrophy locus, followed either by direct isolation of Alu-PCR products or hybridization of Alu-PCR products to chromosome-5-gridded cosmid libraries. 253 human phage and cosmid clones were mapped to various parts of chromosome 5 by deletion mapping to somatic cell hybrid panels. 30 of these clones were mapped into the region 5q11.2-q13.3, 9 of which are flanking rate cutting BssHII-sites, known to be, often, starting points for genes. They represent excellent starting material for the development of new polymorphic markers and sequence-tagged sites, for YAC screening and building of contigs, as well as for direct isolation of genes.
Wirth
B
B
Institute of Human Genetics, University of Bonn, FRG.
Schönling
J
J
el-Agwany
A
A
Seehafer
J
J
Rüther
K
K
Raschke
H
H
Leutner
A
A
Piechaczek-Wappenschmidt
B
B
D000818:Animals; D001345:Autoradiography; D001435:Bacteriophages; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D006224:Cricetinae; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D009134:Muscular Atrophy, Spinal / Q000235:genetics; D016133:Polymerase Chain Reaction
D005819:Genetic Markers