title: Assessment of vitamin B2 status in performance athletes of various types of sports.
abstract: To determine the vitamin B2 supply, 62 athletes in various types of sports and 16 non-athletes were examined, using a weighed food record (7-day), alpha-EGR determination, microbiological blood, and HPLC urinary measurements. If the guidelines of 1.5 or 1.7 mg/day vitamin B2 or 0.6 mg/4,184 kJ (GRDA) are defined for adequate status, all sports groups (M +/- SEM) are above this level. The vitamin B2 blood concentrations show a range (mean) from 303-372 nmol/liter, which indicates that more than 90% of the athletes are above the reference value of 327 nmol/liter (mean). There is a correlation of r = 0.49 between the vitamin B2 intake (mean over 7 days) and the vitamin B2 blood level. Determination of vitamin B2 blood concentration is characterized by good precision (C.V. +/- 3.2%) and recovery (95.7%). The enzyme activation measurement (alpha-EGR) shows that all of the athletes are below the alpha-EGR reference value of < 1.50 (good supply). No significant correlations could be determined between alpha-EGR and vitamin B2 intake, blood concentrations, and urinary excretion. The measurement also shows high precision (C.V. 1.7%). The Vitamin B2 excretion in urine (mumol/g Cr.) was higher than the reference values for non-athletes in more than 90% of the athletes. There is a correlation of r = 0.89 between the vitamin B2 intake on the last day (Day 7) of the food record and the riboflavin excretion, and between vitamin B2 in blood and urinary excretion of r = 0.52. The C.V. of vitamin B2 urinary measurement using HPLC is 2.5%, with recovery of 98.2%. Combined measurement of vitamin B2 intake, blood concentration, and urine excretion appears to be a suitable procedure for determining the vitamin B2 status. Most of the performance athletes were sufficiently supplied with vitamin B2, compared to the reference values for non-athletes.
authors.affiliation: University Medical Hospital, Department of Sport and Performance Medicine, Albert-Ludwigs-University, Freiburg, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Rokitzki
authors.forename: A
authors.initials: A
authors.lastname: Sagredos
authors.forename: E
authors.initials: E
authors.lastname: Keck
authors.forename: B
authors.initials: B
authors.lastname: Sauer
authors.forename: J
authors.initials: J
authors.lastname: Keul
mesh_terms: D000293:Adolescent; D000328:Adult; D002149:Energy Intake; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D009752:Nutritional Status; D012016:Reference Values; D012256:Riboflavin / Q000008:administration & dosage / Q000097:blood* / Q000652:urine; D013177:Sports; D014805:Vitamin B 12
chemical_list: D005980:Glutathione Reductase; D014805:Vitamin B 12; D012256:Riboflavin