Potassium selective and venturicidin sensitive conductances of Fo purified from bovine heart mitochondria, reconstituted in planar lipid bilayers.
Purified F(o), isolated from bovine heart mitochondria was reconstituted into planar lipid bilayers. Two cation selective conductances could be identified. Most frequently, incorporation of F(o) resulted in a voltage sensitive K+ channel of 18 pS. Venturicidin drastically decreased the open probability of this channel. The second conductance of 47 pS had lost its voltage sensitivity but had retained the sensitivity to venturicidin. The F(o) preparation is very pure and will be used in crystallography studies. However, despite the high degree of biochemical purity, the preparation gives rise to several distinct conductances. Therefore, we conclude that for structure/function analysis of the F(o) part a biophysical characterization is required to assess the homogeneity of the preparation.
Miedema
H
H
Department of Plant Physiology and Biochemistry, Vrije Universiteit Amsterdam, The Netherlands.
van Walraven
H S
HS
de Boer
A H
AH
D000818:Animals; D002412:Cations; D002417:Cattle; D002712:Chlorides / Q000378:metabolism; D004553:Electric Conductivity; D008051:Lipid Bilayers / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000201:enzymology*; D011188:Potassium / Q000378:metabolism / Q000494:pharmacology*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D014698:Venturicidins / Q000494:pharmacology*
D002412:Cations; D002712:Chlorides; D008051:Lipid Bilayers; D014698:Venturicidins; D006180:Proton-Translocating ATPases; D011188:Potassium