Intragenic rearrangements in the mitochondrial NADH dehydrogenase subunit 6 gene of vertebrates.
We have sequenced the mitochondrial-encoded NADH dehydrogenase subunit 6 gene from 19 species of birds. Comparison of the derived amino-acid sequences in 22 avian species, six mammals, and two fishes, reveals an intragenic rearrangement in mammals. The C-terminal half of the mammalian protein includes an internal insertion of 10-15 amino acids and a C-terminal deletion of 8-9 amino acids. Based on comparative sequence alignments and hydropathy profile analysis, five hydrophobic segments (designated I to V) corresponding to transmembrane regions are proposed. In this structural model of NADH dehydrogenase subunit 6, the mammalian insertion is found in a variable loop region between transmembrane segments IV and V.
Moum
T
T
Institute of Medical Biology, University of Tromsø, Norway.
Willassen
N P
NP
Johansen
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial; D005796:Genes; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014714:Vertebrates / Q000145:classification / Q000235:genetics*
D004272:DNA, Mitochondrial; D009245:NADH Dehydrogenase