title: Determination of sulphur amino acids by gas chromatography with flame photometric detection.
abstract: A selective and sensitive method has been developed for the determination of sulphur amino acids by gas chromatography (GC). Sulphur amino acids were converted into their N(S)-isopropoxycarbonyl methyl ester derivatives and measured by GC with flame photometric detection using a DB-17 capillary column. The derivatives were sufficiently volatile and stable to give single symmetrical peaks. The detection limits of sulphur amino acids were ca. 0.5-1 pmol per injection, and the calibration curves were linear in the range 0.5-10 nmol for each sulphur amino acid. This method was successfully applied to small urine samples without prior clean-up, and sulphur amino acids in these samples could be analysed without any influence from coexisting substances. Overall recoveries of sulphur amino acids added to urine samples were 85-113%. The analytical results of free sulphur amino acid contents in urine samples of normal subjects are presented.
authors.affiliation: Faculty of Pharmaceutical Sciences, Okayama University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Fujimoto
authors.forename: I
authors.initials: I
authors.lastname: Noguchi
authors.forename: M
authors.initials: M
authors.lastname: Makita
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D003540:Cystathionine / Q000032:analysis / Q000652:urine; D003545:Cysteine / Q000032:analysis / Q000652:urine; D003555:Cystinuria / Q000652:urine; D005260:Female; D006710:Homocysteine / Q000032:analysis / Q000652:urine; D006712:Homocystinuria / Q000652:urine; D006801:Humans; D008297:Male; D008715:Methionine / Q000032:analysis / Q000652:urine; D012016:Reference Values; D012680:Sensitivity and Specificity; D013455:Sulfur
chemical_list: D000596:Amino Acids; D006710:Homocysteine; D003540:Cystathionine; D013455:Sulfur; D008715:Methionine; D003545:Cysteine