Phagocyte transport as mechanism for enhanced therapeutic activity of liposomal amphotericin B.
Liposomal amphotericin B (L-AmB) is emerging as one of the most attractive new antifungal agents. We have attempted to show that phagocytic cells circulating in blood play an important role in transport and accumulation of L-AmB at inflammatory sites in vivo. Free AmB or L-AmB was injected intravenously to mice, and the amount of AmB in peritoneal exudate cells was quantitated by high-performance liquid chromatography. Higher levels of AmB were detected in a higher number of mice injected with L-AmB. The presence of L-AmB in inflammatory peritoneal cells after intravenous administration of fluorescence-labeled L-AmB also suggested that macrophages play an important role in the transport of intravenously administered L-AmB to inflammatory sites.
Mehta
R T
RT
Immunobiology and Drug Carriers Section, University of Texas M.D. Anderson Cancer Center, Houston 77030.
McQueen
T J
TJ
Keyhani
A
A
López-Berestein
G
G
D000666:Amphotericin B / Q000008:administration & dosage* / Q000378:metabolism; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers; D005122:Exudates and Transudates / Q000166:cytology; D005453:Fluorescence; D006801:Humans; D007275:Injections, Intravenous; D008081:Liposomes; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice
D004337:Drug Carriers; D008081:Liposomes; D000666:Amphotericin B