[Effect of female sex steroids on the ability of splenocytes to form an adoptive immune response. Role of prostaglandin F2alpha in the mechanisms of hormonal immunoregulation].
Male CBA mice were used in experiments. Splenocytes were incubated for an hour in macrocultures with estradiol (E2) or progesterone (P), then the cells were transferred (together with the antigen) to lethally irradiated syngeneic recipients, and on day 4 the count of antibody-producing cells (APC) was estimated. Prostaglandin F2 alpha (PGF2 alpha) concentrations were radioimmunoassayed in cell culture supernatants. E2 and P concentrations corresponded to these hormones levels in blood sera during pregnancy. E2 and P in the tested concentrations were found to reliably stimulate the processes of APC formation, their effects being dose independent. Both E2 and P statistically reliably increased PGF2 alpha level in splenocyte culture. Fractionation of splenocytes helped reveal the highest PGF2 alpha level in the cultures devoid of adhesive cells and rich for T lymphocytes. Hence, E2 and P stimulated the processes of antigen-independent differentiation of splenocytes producing APC either by directly stimulating T lymphocytes or via macrophages by blocking their negative effects. Stimulation of adoptive immune response by sex steroids is closely connected with PGF2 alpha production by immunocompetent cells under the effects of these hormones.
Shirshev
S V
SV
Shilov
Iu I
IuI
Kevorkov
N N
NN
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D015237:Dinoprost / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D016219:Immunotherapy, Adoptive; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011374:Progesterone / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D011374:Progesterone; D004958:Estradiol; D015237:Dinoprost
Vliianie zhenskikh polovykh steroidov na sposobnost' splenotsitov formirovat' adoptivnyĭ immunnyĭ otvet. Rol' prostaglandina F2alpha v mekhanizmakh gormonal'noĭ immunoreguliatsii.