Differential intracellular mechanisms mediate the co-ordinate induction of c-fos and jun-B in the rat pineal gland.
In the rat pineal gland the immediate early genes c-fos and jun-B are co-ordinately induced in vivo following the onset of darkness, and in vitro following treatment with adrenergic drugs. An extensive in vitro analysis of the mechanisms which regulate the induction of these two genes has revealed that, although there are common pathways of control, c-fos and jun-B exhibit differential regulation by adrenoceptor types, second messenger pathways and protein kinase C. The presence of differential intracellular mechanisms of regulation provides precise control over the expression pattern of immediate early genes, a pattern which is crucial for cellular response since the combinatorial actions of the products of these genes are a determinant of target gene activation. The pineal gland is a unique experimental model in which the molecular pharmacology of immediate early gene regulation can be investigated.
Carter
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DA
Neuropeptide Laboratory, National University of Singapore.
D000322:Adrenergic Agonists / Q000494:pharmacology; D000818:Animals; D015152:Blotting, Northern; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D016762:Genes, fos; D016758:Genes, jun; D008297:Male; D009924:Organ Culture Techniques; D010870:Pineal Gland / Q000187:drug effects / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015290:Second Messenger Systems; D013565:Sympatholytics / Q000494:pharmacology; D015533:Transcriptional Activation
D000322:Adrenergic Agonists; D012333:RNA, Messenger; D013565:Sympatholytics; D011493:Protein Kinase C