title: Germ line transmission of a yeast artificial chromosome spanning the murine alpha 1(I) collagen locus.
abstract: Molecular complementation of mutant phenotypes by transgenic technology is a potentially important tool for gene identification. A technology was developed that allows the transfer of a physically intact yeast artificial chromosome (YAC) into the germ line of the mouse. A purified 150-kilobase YAC encompassing the murine gene Col1a1 was efficiently introduced into embryonic stem (ES) cells via lipofection. Chimeric founder mice were derived from two transfected ES cell clones. These chimeras transmitted the full length transgene through the germ line, generating two transgenic mouse strains. Transgene expression was visualized as nascent transcripts in interphase nuclei and quantitated by ribonuclease protection analysis. Both assays indicated that the transgene was expressed at levels comparable to the endogenous collagen gene.
authors.affiliation: Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142.
authors.forename: W M
authors.initials: WM
authors.lastname: Strauss
authors.forename: J
authors.initials: J
authors.lastname: Dausman
authors.forename: C
authors.initials: C
authors.lastname: Beard
authors.forename: C
authors.initials: C
authors.lastname: Johnson
authors.forename: J B
authors.initials: JB
authors.lastname: Lawrence
authors.forename: R
authors.initials: R
authors.lastname: Jaenisch
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D015139:Blotting, Southern; D015825:Chromosomes, Fungal; D003094:Collagen / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D017403:In Situ Hybridization; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014162:Transfection
chemical_list: D003094:Collagen