Sequencing and characterization of the kinesin-related genes katB and katC of Arabidopsis thaliana.
Complementary DNAs of two kinesin-related genes, katB and katC, were isolated from Arabidopsis thaliana and sequenced. The carboxyl-terminal regions of the polypeptides encoded by these genes, especially the presumptive ATP-binding and microtubule-binding domains, share significant sequence homology with the mechanochemical motor domain of the kinesin heavy chain. The predicted secondary structures of KatB and KatC proteins include a large globular domain in the carboxyl-terminal region and a small globular domain in the amino-terminal region that are separated by a long alpha-helical coiled-coil with heptad repeats. A truncated KatC polypeptide (KatC(207-754)), which includes the carboxyl-terminal region of KatC, was expressed in Escherichia coli and was shown to possess microtubule-stimulated ATPase activity and to bind to microtubules in an ATP-sensitive manner, both of which are characteristics of kinesin and kinesin-like proteins.
Mitsui
H
H
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Nakatani
K
K
Yamaguchi-Shinozaki
K
K
Shinozaki
K
K
Nishikawa
K
K
Takahashi
H
H
D000251:Adenosine Triphosphatases / Q000032:analysis; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D016547:Kinesins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D008870:Microtubules / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D011994:Recombinant Proteins; D000251:Adenosine Triphosphatases; C089152:KatB protein, Arabidopsis; C089153:KatC protein, Arabidopsis; D016547:Kinesins