Cytogenetic characterization of a periampullary adenocarcinoma of the pancreas, its liver metastasis, and a cell line established from the metastasis and a cell line established from the metastasis in a patient with Gardner's syndrome.
A cell line was established from a liver metastasis of a periampullary pancreatic carcinoma in a patient with Gardner's syndrome. The primary tumor, the liver metastasis, and passages 6 and 15 of the cell line were characterized cytogenetically. The only aberration common to all samples was a der(15)t(8;15); this was probably the primary chromosomal abnormality. Loss of the short arm of chromosome 19 was also found in all samples but was brought about by different aberrations in the primary tumor and the metastasis. The secondary aberrations characteristic of clonal evolution often included further gain of 8q material but losses from 1p, 6q, and chromosomes 17 and 18, all of which have been seen before in sporadic pancreatic and colorectal carcinomas. This is the first cell line established from a tumor in a Gardner's syndrome patient and also the first characterization of an abnormal tumor karyotype associated with this autosomal dominant cancer syndrome.
Bardi
G
G
Department of Clinical Genetics, University Hospital, Lund, Sweden.
Aman
P
P
Johansson
B
B
Pandis
N
N
Mandahl
N
N
Bak-Jensen
E
E
Björkman
A
A
Sjögren
H O
HO
Andrén-Sandberg
A
A
Mitelman
F
F
D000231:Adenocarcinoma, Papillary / Q000235:genetics* / Q000556:secondary; D014670:Ampulla of Vater; D000818:Animals; D002869:Chromosome Aberrations; D005260:Female; D005736:Gardner Syndrome / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics / Q000556:secondary*; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured