Interleukin 13, a T-cell-derived cytokine that regulates human monocyte and B-cell function.
We have isolated the human cDNA homologue of a mouse helper T-cell-specific cDNA sequence, called P600, from an activated human T-cell cDNA library. The human cDNA encodes a secreted, mainly unglycosylated, protein with a relative molecular mass of approximately 10,000. We show that the human and mouse proteins cause extensive morphological changes to human monocytes with an associated up-regulation of major histocompatibility complex class II antigens and the low-affinity receptor for immunoglobulin E (Fc epsilon RII or CD23). In addition, they stimulate proliferation of human B cells that have been activated by anti-IgM antibodies or by anti-CD40 monoclonal antibodies presented by a mouse Ltk- cell line transfected with CDw32. Furthermore, the human protein induced considerable levels of IgM and IgG, but no IgA production, in cultures in which highly purified human surface IgD+ or total B cells were cocultured with an activated CD4+ T-cell clone. Based on these findings, we propose that this immunoregulatory protein be designated interleukin 13.
McKenzie
A N
AN
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304-1104.
Culpepper
J A
JA
de Waal Malefyt
R
R
Brière
F
F
Punnonen
J
J
Aversa
G
G
Sato
A
A
Dang
W
W
Cocks
B G
BG
Menon
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics / Q000494:pharmacology* / Q000502:physiology; D005434:Flow Cytometry; D015723:Gene Library; D006681:HLA-D Antigens / Q000096:biosynthesis; D006801:Humans; D018793:Interleukin-13; D007378:Interleukins / Q000235:genetics / Q000494:pharmacology* / Q000502:physiology; D004915:Leukemia, Erythroblastic, Acute; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014066:Palatine Tonsil / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017455:Receptors, IgE / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects
D016207:Cytokines; D006681:HLA-D Antigens; D018793:Interleukin-13; D007378:Interleukins; D012333:RNA, Messenger; D017455:Receptors, IgE; D011994:Recombinant Proteins