Determination of 9 alpha, 11 beta-prostaglandin F2 in human urine and plasma by gas chromatography-mass spectrometry.
Sensitive and specific assay methods for 9 alpha, 11 beta-prostaglandin F2 (9 alpha, 11 beta-PGF2) by gas chromatography-mass spectrometry with electron impact ionization are described. The mass spectrometric assay for 9 alpha, 11 beta-PGF2 was based on the use of the methyl ester-dimethylisopropylsilyl ether derivative, and pentadeuterated PGF2 alpha as a convenient internal standard. The calibration graph for 9 alpha, 11 beta-PGF2 was linear from 5 pg to 100 ng for both the standard and spiked biological samples. The limit of detection was 50 pg/ml for urine and 25 pg/ml for plasma (signal-to-noise ratio = 2.3). The method was applied to the determination of 9 alpha, 11 beta-PGF2 in urine and plasma samples from patients with bronchial asthma.
Obata
T
T
Division of Biochemistry, Jikei University School of Medicine, Tokyo, Japan.
Nagakura
T
T
Kammuri
M
M
Masaki
T
T
Maekawa
K
K
Yamashita
K
K
D000293:Adolescent; D002648:Child; D015237:Dinoprost / Q000097:blood* / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D006801:Humans; D012680:Sensitivity and Specificity
D015237:Dinoprost