title: The regulation of membrane 125I- and 86Rb+ permeability in a virally transformed cell line (NCL-SG3) derived from the human sweat gland epithelium.
abstract: We have explored the factors that may regulate membrane permeability in a cell line (NCL-SG3) derived from the human sweat gland epithelium. Ionomycin increased the rate of 125I-efflux from preloaded cells and this action appeared to be due to an increase in intracellular free calcium ([Ca2+]i). The ionomycin-evoked increase in 125I- efflux was reduced in cells that were exposed either to barium or to valinomycin in the presence of a high concentration of external potassium. It thus appears that a fraction of the ionomycin-evoked increase in 125I- efflux is due to the activation of potassium channels and experiments using 86Rb+ also suggested that ionomycin increased the rate of potassium efflux, an effect which was totally abolished by barium. Blockade of Na(+)-K(+)-2Cl- cotransport and of Cl- -HCO3- exchange reduced the basal rate of 125I- efflux and the ionomycin-evoked increase in 125I-efflux from control cells and from cells depolarized by valinomycin. These transport systems thus contribute to anion efflux, although [Ca2+]i-dependent chloride channels also appear to be present. Acetylcholine increases [Ca2+]i in the secretory cells of human sweat glands, but this neurotransmitter did not increase [Ca2+]i in NCL-SG3 cells and so membrane permeability was not under cholinergic control. Adrenaline did not increase [Ca2+]i, but this hormone did evoke cyclic-3',5'-adenosine monophosphate (cyclic AMP) production. However, membrane permeability was not under adrenergic control, as the cells did not appear to express functional, cyclic AMP-dependent anion channels. This may be because they were not fully differentiated under the culture conditions. ATP consistently evoked a dose-dependent increase in anion efflux that appeared to be mediated by [Ca2+]i. The increase in [Ca2+]i was initiated by the release of calcium from a limited internal store and was subsequently sustained by calcium influx. UTP and ADP also increased [Ca2+]i, whereas adenosine, AMP and alpha,beta-methylene ATP were without effect. These data thus suggest that a subclass of type 2 purine receptor, which is functionally coupled to phosphoinositidase C, is present in these cells.
authors.affiliation: Institute of Physiology, University of Glasgow.
authors.forename: S M
authors.initials: SM
authors.lastname: Wilson
authors.forename: M L
authors.initials: ML
authors.lastname: Whiteford
authors.forename: D L
authors.initials: DL
authors.lastname: Bovell
authors.forename: J D
authors.initials: JD
authors.lastname: Pediani
authors.forename: W H
authors.initials: WH
authors.lastname: Ko
authors.forename: G L
authors.initials: GL
authors.lastname: Smith
authors.forename: C M
authors.initials: CM
authors.lastname: Lee
authors.forename: H Y
authors.initials: HY
authors.lastname: Elder
mesh_terms: D002118:Calcium / Q000378:metabolism; D002461:Cell Line, Transformed; D002463:Cell Membrane Permeability; D000242:Cyclic AMP / Q000096:biosynthesis; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D007455:Iodine / Q000493:pharmacokinetics*; D007457:Iodine Radioisotopes; D009994:Osmolar Concentration; D012413:Rubidium / Q000493:pharmacokinetics*; D012414:Rubidium Radioisotopes; D013268:Stimulation, Chemical; D013545:Sweat Glands / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018406:Virus Physiological Phenomena
chemical_list: D007457:Iodine Radioisotopes; D012414:Rubidium Radioisotopes; D007455:Iodine; D000242:Cyclic AMP; D012413:Rubidium; D002118:Calcium