Expression of immunoreactive polysialylated neural cell adhesion molecule in the suprachiasmatic nucleus.
Light-microscopic and immunoblot immunochemical procedures were used to study the distribution and biochemical characteristics of neural cell adhesion molecule (NCAM) and its polysialylated form (PSA-NCAM) in the suprachiasmatic nuclei (SCN) of the adult Siberian hamster. In the adult brain PSA-NCAM is located in regions capable of undergoing morphological rearrangements and thus is generally considered to be an indicator of neural plasticity. Immunostaining for PSA in the Siberian hamster SCN (using a monoclonal antibody against the alpha 2,8-linked PSA of NCAM) was evident throughout the rostrocaudal axis of the SCN, with the most intense reaction in the ventrolateral region. Immunoreactivity was present in the neuropil, which delineated groups of cells with unstained cytoplasm. Many of the SCN cells were aggregated into cords or clusters. The optic chiasm was free from label, except for short processes apparently extending from the densely stained neuropil in the ventrolateral SCN. Immunoreactivity was abolished by preincubating sections in an endoneuraminidase (endo-N) or by preincubation of the primary antibody with PSA-NCAM. Immunostaining of the nonsialylated NCAM polypeptide was also limited to the neuropil, but this was more diffuse and less regionally specific than PSA staining. The ventral SCN exhibited the most intense labeling for NCAM. Immunoblot analyses revealed the immunoreactive PSA-NCAM as a broad band migrating between apparent molecular weights in the range of 150-300 kD, which was ablated by treatment of SCN tissue extract with endo-N. This electrophoretic migration pattern of PSA-NCAM from the SCN region was similar to that seen in several other brain regions.(ABSTRACT TRUNCATED AT 250 WORDS)
Glass
J D
JD
Department of Biological Sciences, Kent State University, OH 44242.
Lee
W
W
Shen
H
H
Watanabe
M
M
D000818:Animals; D001923:Brain Chemistry; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis* / Q000378:metabolism; D006224:Cricetinae; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D017111:Phodopus; D012794:Sialic Acids / Q000032:analysis* / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000737:chemistry*; D014018:Tissue Distribution
D015816:Cell Adhesion Molecules, Neuronal; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid