[The relationship between the major components of extracellular matrix and growth, motility and invasion in mice pulmonary adenocarcinoma].
After mouse pulmonary adenocarcinoma cell line LA795 was implanted in the subrenal capsule of T739 mice, the local distribution of fibronectin (FN), laminin (LN) and type IV collagen was detected by indirect immunoperoxidase and immunofluorescence staining and were observed during tumor growth at different time intervals. As the tumors progressed, it was shown that the expression of FN decreased while that of LN and type IV collagen gradually increased, their distribution patterns were also different. LN and type IV collagen were absent in the basement membrane in the tumor invasion areas. In order to verify the in vivo observations, various in vitro methods were used. We found expression of FN and LN but no expression of type IV collagen mRNA in LA795 cell line. Assay of random migration of tumor cells was employed to check the influence of FN, LN or type IV collagen on the motility of tumor cells. It was demonstrated that LN, FN, type IV collagen and in particular, LN could promote the motility of tumor cells individually. Cultured with addition of LN or FN, tumor cell colonies expanded diametrically more rapidly. The results indicated that FN, LN and type IV collagen may contribute to tumor invasion and metastasis.
Wang
F
F
Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
Gao
J
J
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009361:Neoplasm Invasiveness; D013370:Subrenal Capsule Assay; D014407:Tumor Cells, Cultured
D005353:Fibronectins; D007797:Laminin; D003094:Collagen