Expression of functional human CSF-1 receptors on mouse bone marrow erythroid progenitor cells.
We have previously shown that a murine multipotent hematopoietic cell line could proliferate in response to CSF-1 after retroviral transfer of human CSF-1 receptor (CSF-1R) gene (c-fms) without loss of the erythroid differentiation potential. In the light of these data, we asked whether ectopic expression of human c-fms gene would lead to the conditional immortalization of murine hematopoietic progenitor cells. In the present studies, murine bone marrow cells were infected with recombinant retroviruses containing the human c-fms cDNA. We found that CSF-1 could exert a stimulatory activity on erythroid progenitors in the presence of Epo only when bone marrow cells had been previously infected with c-fms retroviruses. In addition, expression of human CSF-1R in murine Epo-dependent, v-src-immortalized cells resulted in CSF-1-dependent proliferation of these cells in the absence of Epo. These data show that human CSF-1R (i) can stimulate early bone marrow erythroid progenitors, (ii) might require additional signals provided by oncogenes or cytokine receptors to transduce a mitogenic signal into mouse bone marrow erythroid progenitors.
Mouchiroud
G
G
Centre de Génétique Moléculaire et Cellulaire, UMR CNRS 106, Université Claude-Bernard, Villeurbanne, France.
Bourette
R
R
Blanchet
J P
JP
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000166:cytology*; D004921:Erythropoietin / Q000494:pharmacology; D015870:Gene Expression; D016236:Genes, fms / Q000235:genetics; D006801:Humans; D051379:Mice; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics*
D004921:Erythropoietin; D016186:Receptor, Macrophage Colony-Stimulating Factor