Regional specificity of long-term regulation of tyrosine hydroxylase in some catecholaminergic rat brainstem areas. II. Effect of a chronic dihydralazine treatment.
Dihydralazine, which is used in the treatment of hypertension, causes a long-lasting hypotensive action by a direct vasodilator effect on arteriolar smooth muscle. The present study was carried out to investigate the effect of a daily single injection of dihydralazine (20 mg/kg, s.c.) for 14 days on the tyrosine hydroxylase (TH) protein quantity in some catecholaminergic rat brainstem areas such as the dorsomedial medulla (DMM), the ventrolateral medulla (VLM) and the locus coeruleus (LC). This study demonstrates that the dihydralazine produced (1) an 85% increase in TH protein quantity exclusively in the rostral part of DMM, (2) a 58% increase of TH protein content exclusively in the rostral part of the LC, and (3) a 37% increase of the TH protein quantity in VLM catecholaminergic area. To determine whether the increase in TH protein quantity could be related to a change in norepinephrine (NE) content, the rate constant of disappearance (k) of NE was measured in the catecholaminergic regions of the same rats treated with dihydralazine. Our results show that dihydralazine causes an increase of the TH protein, in addition to an elevation of NE content, within the subpopulations of catecholaminergic structures. These data suggest a selective response of the TH regulation to dihydralazine within the rostral DMM area which receives barosensory inputs.
Schmitt
P
P
Laboratoire de Neuropharmacologie Moléculaire, UMR 105 CNRS-UCB, Faculté de Médecine Alexis Carrel, Lyon, France.
Pequignot
J
J
Hanchin
F
F
Pujol
J F
JF
Pequignot
J M
JM
D000818:Animals; D001933:Brain Stem / Q000187:drug effects / Q000201:enzymology*; D002395:Catecholamines / Q000502:physiology*; D004078:Dihydralazine / Q000494:pharmacology*; D007700:Kinetics; D008125:Locus Coeruleus / Q000201:enzymology; D008297:Male; D008526:Medulla Oblongata / Q000201:enzymology; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000187:drug effects*
D002395:Catecholamines; D014446:Tyrosine 3-Monooxygenase; D004078:Dihydralazine; D009638:Norepinephrine