Measuring H type 1 and H type 2 antigens in human saliva by immunoassay using artificial antigens as standard substances.
A time-resolved fluorometric immunoassay for water-soluble H antigens of ABH blood group substances has been developed. Introducing artificial antigens (trisaccharide-albumin complexes for H type 1 and H type 2) as reference substances, H blood group substances in human saliva were measured. The monoclonal antibody, anti-H 1E3, reacted with both H type 1 and type 2 chains, and the commercially available anti-H reacted with H type 2 chain. Using these two antibodies we found 10-20-fold higher concentration of H type 1 compared with that of H type 2 in human saliva. The H type 2 was not found in the saliva samples from nonsecretors, irrespective of ABO phenotypes. The results suggest that approximately 90% of A and B blood group substances in human saliva are built on type 1 chains.
Wang
B
B
Department of Legal Medicine, Kurume University School of Medicine, Fukuoka, Japan.
Akiyama
K
K
Jia
J T
JT
Kimura
H
H
D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D010641:Phenotype; D012015:Reference Standards; D012016:Reference Values; D012463:Saliva / Q000276:immunology*; D012710:Serum Albumin, Bovine; D014312:Trisaccharides
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000941:Antigens; C089307:Syntagen H type 2; C089306:Syntagen Le(d) (H type 1); D014312:Trisaccharides; D012710:Serum Albumin, Bovine