Estimate of the genetic divergence between inbred Dahl salt-sensitive and salt-resistant rats.
OBJECTIVE
The genetic divergence of inbred Dahl salt-sensitive (SS/Jr) rats from inbred Dahl salt-resistant (SR/Jr) rats and various other inbred strains was measured.


METHODS
Structural differences in DNA between strains were evaluated.


METHODS
Genetic variants were sought (1) by restriction fragment length polymorphism (RFLP) analysis, using 19 DNA probes, (2) by the polymerase chain reaction around microsatellites and (3) by DNA sequencing.


RESULTS
It was estimated that 1 in 1532 bases of DNA were different between the SS/Jr and SR/Jr strains. In comparing SS/Jr and SR/Jr rats, it was also observed that one DNA probe in 10 will yield multiple RFLP, presumably as the result of large insertion/deletion events. A comparison of SS/Jr rats with seven other inbred strains showed that the percentage of loci that carry alleles different from SS/Jr rats varies from about 23% for Albino Surgery rats to 71% for Brown Norway rats.


CONCLUSIONS
Although the SR/Jr strain is an appropriate contrasting strain for the genetic analysis of hypertension in SS/Jr rats, a genetic analysis involving crosses of SS/Jr rats and unrelated inbred strains is also likely to be useful in identifying genes that cosegregate with blood pressure because more informative genetic markers will be available than in a cross of SS/Jr with SR/Jr rats.
Ginn
D I
DI
Department of Medicine, Medical College of Ohio, Toledo 43699.
Baptista
C A
CA
Alam
K Y
KY
Deng
A Y
AY
Cicila
G T
GT
Margolius
H S
HS
Rapp
J P
JP
D000818:Animals; D014644:Genetic Variation; D006973:Hypertension / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*; D012083:Renin / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology*
D012965:Sodium Chloride; D012083:Renin