title: Structure of the nucleocapsid protein gene of rice yellow stunt rhabdovirus.
abstract: The complete nucleotide sequence of the N protein gene of rice yellow stunt rhabdovirus (RYSV) was determined by sequencing of cDNA clones derived from the viral genomic RNA. The 3' end of the N gene (messenger sense) was defined by sequence analysis of cDNA clones generated from the N protein mRNA by 3'RACE. The 5' end sequence of the gene was putatively assigned as 5'-AACAC-3'; this sequence is found in the presumed 3' leader/N gene junction region. The mRNA encoding the RYSV N protein is 1714 nt comprising a 15-nt untranslated 5' leader sequence followed by an open reading frame (ORF) of 1563 nt and a 136-nt untranslated 3' region. The calculated molecular mass of the N protein encoded by the ORF is 58,400 Da, which is larger in size than N proteins of other rhabdoviruses. Amino acid composition analysis shows that the RYSV N protein is rather basic with a predicted isoelectric point of 10.04; indeed, a large highly basic region could be found at the carboxy terminal portion of the protein. Amino acid sequence comparison between N proteins of RYSV and sonchus yellow net virus, both of which belong to the same genus Nucleorhabdovirus, revealed an overall 30% identity, with three relatively conserved blocks of 14-20 amino acid residues. Moreover, the hydropathy profiles of the two proteins are generally similar. The structural similarities between the N protein of RYSV and that of lettuce necrotic yellows virus, the type member of the genus Cytorhabdovirus, and those of animal rhabdoviruses, are less significant. Nucleotide sequence determination of 5' and 3' regions flanking the RYSV N gene identified a 14-nt common sequence that is very similar to the consensus gene junction sequences of other plant and animal rhabdoviruses.
authors.affiliation: Department of Virology, Institute of Microbiology, Academia Sinica, Beijing, China.
authors.forename: R X
authors.initials: RX
authors.lastname: Fang
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: B Y
authors.initials: BY
authors.lastname: Xu
authors.forename: Z
authors.initials: Z
authors.lastname: Pang
authors.forename: H T
authors.initials: HT
authors.lastname: Zhu
authors.forename: K Q
authors.initials: KQ
authors.lastname: Mang
authors.forename: D M
authors.initials: DM
authors.lastname: Gao
authors.forename: W S
authors.initials: WS
authors.lastname: Qin
authors.forename: N H
authors.initials: NH
authors.lastname: Chua
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral; D000918:Antibody Specificity; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015723:Gene Library; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D010942:Plant Viruses / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D015183:Restriction Mapping; D012209:Rhabdoviridae / Q000235:genetics*; D016415:Sequence Alignment; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D000914:Antibodies, Viral; D018076:DNA, Complementary; D012333:RNA, Messenger; D012367:RNA, Viral; D014758:Viral Core Proteins; D015678:Viral Structural Proteins