Inhibitors of the conformational switch involved in CD4 binding by the env glycoprotein gp120 from human immunodeficiency virus type 1 (HIV1).
A 15-residue fragment within the major continuous domain of gp120 from HIV1 that can bind independently to the CD4 receptor conserves the property of behaving as a polarity-triggered conformational switch despite displaying over 50% variability between strains. As this switch behavior (the ability to flip abruptly from beta-sheet to alpha-helix as the medium polarity is lowered past a critical point) is closely linked to CD4-binding ability, it presents a potential strain-independent target for intervention. A number of compounds have been tested for their ability to function as switch inhibitors. All those that displayed switch inhibitory activity also have been shown to act to prevent CD4 binding and/or viral infectivity. In addition, all compounds testing positive as switch inhibitors have certain chemical characteristics in common. The groundwork has thus been established for the design of strain-independent blockers of CD4 binding based on the strategy of switch inhibition.
Reed
J
J
Department of Pathochemistry, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Kinzel
V
V
D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000494:pharmacology*; D001593:Benzyl Compounds / Q000494:pharmacology; D015704:CD4 Antigens / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D004151:Dipeptides / Q000494:pharmacology*; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects
D000998:Antiviral Agents; D001593:Benzyl Compounds; D015704:CD4 Antigens; D004151:Dipeptides; D015699:HIV Envelope Protein gp120; C067667:N-carbomethoxycarbonyl-prolyl-phenylalanyl-benzyl ester