title: T cell differentiation and generation of the antigen-specific T cell repertoire in man: observations in MHC class II deficiency.
abstract: The circulating T cell pool of an MHC class II-deficient patient was shown to lack the MHC class II-specific T cell functions. This was demonstrated by the absence of MHC class II-specific alloreactive T cells and a substantially decreased number of circulating CD4+ lymphocytes. The patient's T cells did respond to an allostimulus, although the restriction pattern of this reaction remains speculative. The function and distribution of peripheral T cell subsets from the patient resemble findings in MHC class II-deficient mice, which also lack interaction of T cell precursors with MHC class II-bearing accessory cells during thymic differentiation. Our data support the concept that T cell differentiation in humans is similar, and that the human MHC-restricted T cell repertoire depends on prior interaction of T cell precursors with self MHC.
authors.affiliation: Institute of Immunology, University of Vienna, Austria.
authors.forename: J W
authors.initials: JW
authors.lastname: Mannhalter
authors.forename: H M
authors.initials: HM
authors.lastname: Wolf
authors.forename: I
authors.initials: I
authors.lastname: Hauber
authors.forename: M
authors.initials: M
authors.lastname: Miricka
authors.forename: H
authors.initials: H
authors.lastname: Gadner
authors.forename: M M
authors.initials: MM
authors.lastname: Eibl
mesh_terms: D001483:Base Sequence; D002454:Cell Differentiation; D002648:Child; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex / Q000276:immunology*; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D016511:Severe Combined Immunodeficiency / Q000235:genetics / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*
chemical_list: D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D012333:RNA, Messenger