title: Overexpression of TGN38/41 leads to mislocalisation of gamma-adaptin.
abstract: TGN38 and TGN41 are isoforms of a monotopic integral membrane protein which recycles between the trans Golgi network (TGN) and the cell surface, but which, at steady state, is predominantly located in the TGN. Full-length and truncated versions of rat TGN38/41 have been expressed in monkey (COS) and human (Heb7a) cells under the control of the heavy metal inducible Metallothionein IIA promoter. This has allowed the regulated expression of TGN38/41 protein constructs to different levels in the transfected cells. These studies show that (i) controlled overexpression of TGN38/41 results in mislocalisation to parts of the endocytic pathway, (ii) a truncated version of TGN38/41, lacking the cytoplasmic domain, remains in the TGN, and (iii) there is a direct or indirect interaction between the cytoplasmic domain of TGN38/41 and gamma-adaptin.
authors.affiliation: Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, UK.
authors.forename: B
authors.initials: B
authors.lastname: Reaves
authors.forename: G
authors.initials: G
authors.lastname: Banting
mesh_terms: D033982:Adaptor Protein Complex gamma Subunits; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020126:Brefeldin A; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003517:Cyclopentanes / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009838:Oligodeoxyribonucleotides; D051381:Rats; D017384:Sequence Deletion; D013347:Subcellular Fractions / Q000378:metabolism; D014162:Transfection
chemical_list: D033982:Adaptor Protein Complex gamma Subunits; D003517:Cyclopentanes; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009838:Oligodeoxyribonucleotides; C072237:TGOLN2 protein, human; C513045:Tgoln2 protein, rat; D020126:Brefeldin A