Chromosomal localization and structure of the human type II IMP dehydrogenase gene (IMPDH2).
We determined the chromosomal localization and structure of the gene encoding human type II inosine 5'-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205), an enzyme associated with cellular proliferation, malignant transformation, and differentiation. Using polymerase chain reaction (PCR) primers specific for type II IMPDH, we screened a panel of human-Chinese hamster cell somatic hybrids and a separate deletion panel of chromosome 3 hybrids and localized the gene to 3p21.2-->p24.2. Two overlapping yeast artificial chromosome clones containing the full gene for type II IMPDH (IMPDH2) were isolated, and a physical map of 117 kb of human genomic DNA in this region of chromosome 3 was constructed. The gene for type II IMPDH was localized and oriented on this map and found to span no more than 12.5 kb.
Glesne
D
D
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Collart
F
F
Varkony
T
T
Drabkin
H
H
Huberman
E
E
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D007168:IMP Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004247:DNA; D007168:IMP Dehydrogenase