[Preparation of membrane proteins of Trichinella spiralis larva and application of them in detection with ELISA].
Membrane proteins of muscle larva of Trichinella spiralis (T.s) were isolated by using the non-ionic detergent Triton X-114. At least fourteen components in the membrane proteins were separated with SDS-PAGE and then silver nitrate stain. The bands of them were located between 99-18kd MW level. The MW of chief bands were 94, 90, 47, 32 and 28kd. The sensitivity and specificity of these proteins were determined with ELISA. They didn't cross react with sera of rabbits infected with Toxocara canis (T. c), Schistosoma japonicum (S. j), Paragonimus skr jabini (P. s) and Clonorchis sinensis (C. s). Their sensitivity is the same as and their specificity is higher than those of ES antigens and cuticular antigens obtained with cetultrimethylamonium bromide (CTAB) from muscle larva of Trichinella spiralis.
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D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D008565:Membrane Proteins / Q000302:isolation & purification*; D051379:Mice; D011817:Rabbits; D017160:Trichinella spiralis / Q000276:immunology*
D000947:Antigens, Helminth; D008565:Membrane Proteins