B-raf oncogene: activation by rearrangements and assignment to human chromosome 7.
cDNA clones presumably containing a transforming gene were isolated from transformed NIH3T3 cells, which were obtained by the transfection of DNA from peripheral blood lymphocytes of a familial adenomatous polyposis patient. Sequence analysis of the cDNA clones showed that the gene contained an activated B-raf, the 5' half of which was replaced by the human small nuclear ribonucleoprotein E protein gene and an unknown gene. A partial genomic physical map of the transforming gene was made on the basis of the physical map of the cDNA clones, indicating that rearrangements had occurred during the transfection. The human B-raf gene was shown to be located on chromosome 7 by Southern blotting analysis of rodent-human somatic cell hybrid DNA using the B-raf cDNA as a probe.
Kamiyama
T
T
Department of Hygiene and Oncology, Tokyo Medical and Dental University School of Medicine.
Aoki
N
N
Yuasa
Y
Y
D016475:3T3 Cells; D011125:Adenomatous Polyposis Coli / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D015723:Gene Library; D015321:Gene Rearrangement; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes; D012150:Polymorphism, Restriction Fragment Length; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf; D011995:Recombination, Genetic; D014162:Transfection / Q000235:genetics
D011518:Proto-Oncogene Proteins; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf