title: Complete sequence of the Bacteroides fragilis YCH46 neuraminidase-encoding gene.
abstract: The nucleotide sequence of the Bacteroides fragilis neuraminidase-encoding gene (nanH) and its flanking regions was determined. The B. fragilis nanH open reading frame (ORF) of 1632 nucleotides encoded a neuroaminidase of 544 amino acid residues with a calculated molecular mass of 59.51 kDa. The amino acid sequence at the N-terminus of the deduced protein exhibited the common features of procaryotic signal sequences. The Asp box sequence, Ser-X-Asp-X-Gly-X-Thr-Trp, which is conserved among bacterial neuraminidases, was found at five positions in the deduced amino acid sequence. A single transcription start point was identified 88 nucleotides upstream of the nanH ORF. Northern blot analysis using an internal nanH probe revealed a single mRNA species with a length of 1.8 kb that could encode just the neuraminidase.
authors.affiliation: Department of Bacteriology, School of Medicine, University of Tokushima, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Akimoto
authors.forename: T
authors.initials: T
authors.lastname: Ono
authors.forename: H
authors.initials: H
authors.lastname: Tsutsui
authors.forename: T
authors.initials: T
authors.lastname: Kinouchi
authors.forename: K
authors.initials: K
authors.lastname: Kataoka
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
mesh_terms: D000595:Amino Acid Sequence; D001441:Bacteroides fragilis / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D016384:Consensus Sequence; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D009439:Neuraminidase / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D009439:Neuraminidase