title: Molecular cloning and characterization of the pgm gene encoding phosphoglucomutase of Escherichia coli.
abstract: We report here the identification and characterization of pgm, a gene in Escherichia coli that encodes the enzyme phosphoglucomutase, specifically required for the catalysis of the interconversion of glucose 1-phosphate and glucose 6-phosphate. The predicted amino acid sequence of the pgm gene is highly conserved in E. coli, Acetobacter xylinum, Saccharomyces cerevisiae, rabbits, and humans. pgm deletion mutant strains are deficient in phosphoglucomutase activity.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138.
authors.forename: M
authors.initials: M
authors.lastname: Lu
authors.forename: N
authors.initials: N
authors.lastname: Kleckner
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002462:Cell Membrane / Q000187:drug effects; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010733:Phosphoglucomutase / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000235:genetics; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014157:Transcription Factors
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C089746:SeqA protein, E coli; D014157:Transcription Factors; D012967:Sodium Dodecyl Sulfate; D010733:Phosphoglucomutase