Removal and inactivation of enveloped and non-enveloped viruses during the purification of a high-purity factor IX by metal chelate affinity chromatography.
Virus reduction during the copper chelate affinity chromatography stage used during the purification of a new high-purity factor IX (BPLs 9MC) has been investigated. Virus reduction for the enveloped virus Sindbis was 6.5 log, a value which included approximately 2 log of inactivation due to the use of an acidic wash buffer (pH 4.4) during chromatography. In the case of the non-enveloped hepatitis-A-like poliovirus, which is acid-resistant, the virus reduction value was 4.0 log and was exclusively due to physical virus removal during the chromatographic process.
Roberts
P L
PL
Bio Products Laboratory, Elstree, UK.
Walker
C P
CP
Feldman
P A
PA
D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods; D003300:Copper; D004340:Drug Contamination / Q000517:prevention & control*; D005164:Factor IX / Q000302:isolation & purification*; D006801:Humans; D010949:Plasma / Q000382:microbiology; D017955:Poliovirus; D012845:Sindbis Virus; D014759:Viral Envelope Proteins
D002614:Chelating Agents; D014759:Viral Envelope Proteins; D003300:Copper; D005164:Factor IX