title: The effect of [Arg8]vasopressin on electrogenic chloride secretion in cultured rat epididymal epithelia.
abstract: Primary cultured rat efferent ductal epithelia and cauda epididymal epithelial were mounted in Ussing chambers to study the effect of arginine vasopressin (AVP) on chloride secretion in the male excurrent duct. The regional differences in the signal transduction pathways involved were also investigated. In both the efferent duct and the cauda epididymidis, basolateral addition of AVP resulted in a dose-dependent increase in the short-circuit current (Isc), which was mediated via V1 receptor. Replacement of ambient Cl- with gluconate or pretreatment of a Cl- channel blocker, diphenylamine-2-carboxylate (apical, 1 mM), completely abolished the response, whereas addition of amiloride had no effect on the Isc. Pretreating the epithelia of the efferent duct with indomethacin (apical, 5 microM) or forskolin (basolateral, 1 microM), but not thapsigargin (apical, 1 microM) or trifluoperazine (apical, 20 microM), significantly inhibited the AVP response (P < 0.001). By comparison, pretreating the epithelia of the cauda epididymidis with any of the four agents significantly reduced the AVP-evoked response. These results suggested that the stimulation of chloride secretion by AVP in the efferent duct and the cauda epididymidis is mediated by prostaglandin synthesis and involves adenosine 3',5'-cyclic monophosphate (cAMP) as a second messenger. In the cauda epididymidis, calcium, in addition to cAMP, may play a role in mediating the AVP-induced response.
authors.affiliation: Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories.
authors.forename: K B
authors.initials: KB
authors.lastname: Lai
authors.forename: W O
authors.initials: WO
authors.lastname: Fu
authors.forename: W H
authors.initials: WH
authors.lastname: Ko
authors.forename: H C
authors.initials: HC
authors.lastname: Chan
authors.forename: P Y
authors.initials: PY
authors.lastname: Wong
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002712:Chlorides / Q000378:metabolism*; D046508:Culture Techniques; D000242:Cyclic AMP / Q000502:physiology; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004594:Electrophysiology; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D004837:Epinephrine / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014268:Trifluoperazine / Q000494:pharmacology
chemical_list: D002712:Chlorides; D001127:Arginine Vasopressin; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D014268:Trifluoperazine; D004533:Egtazic Acid; D000242:Cyclic AMP; D007213:Indomethacin; D004837:Epinephrine