Enhancement of haemopoietic stem cell adherence to bone-marrow derived stroma by phyto-haemagglutinin treated leucocyte conditioned medium (PHA-LCM).
Stem cell adhesion to bone-marrow derived stroma, plays a crucial role in haemopoiesis. However, there is very little information as to the nature of the adhesion molecule. In this paper we have shown that human bone-marrow derived stroma can be established in tissue culture. This stroma is able to adhere human bone-marrow mononuclear cells including the multipotent stem cell, viz. CFU-GEMM. Their adherence increases when the stroma is treated with lymphokines in the form of PHA-treated leucocyte conditioned medium (PHA-LCM). Triton X-100 extracts of the untreated and PHA-LCM treated stroma were analysed on single dimension PAGE. It was observed that PHA-LCM treated stromal extracts showed two extra bands and an increase in the density of a band of approximately 14 kDa. Whether these changes have anything to do with the increased adhesion of stem cell is not yet known.
Desai
S S
SS
Chemotherapy & Stem Cell Biology Division, Tata Memorial Centre, Bombay, India.
Goud
A P
AP
Tendolkar
A G
AG
Rao
S G
SG
D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007962:Leukocytes; D010835:Phytohemagglutinins / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology
D017077:Culture Media, Conditioned; D010835:Phytohemagglutinins