Identification and isolation of omega-conotoxin binding protein from rabbit brain.
N-type of calcium channels belongs to a family of voltage-dependent calcium channels and occurs predominantly in neuronal tissue. One of the basic characteristics of this channel type is binding of omega-conotoxin GVIA--a potent blocker of the calcium current through this type of channel. We have found that two proteins with Mw 170-175 kD and 45-60 kD possess omega-conotoxin GVIA binding sites, while the only protein with Mw 170-175 kD was able to transport calcium after reconstitution into the phospholipid vesicles. The same results were obtained from human embryonic brain. Our results suggest that protein with Mw 170-175 kD corresponds to the alpha 1-subunit of N-type of voltage-dependent calcium channel.
Hudecová
S
S
Department of Molecular Biology, Comenius University, Bratislava, Slovak Republic.
Krizanová
O
O
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D017136:Ion Transport; D008297:Male; D010455:Peptides / Q000378:metabolism*; D011817:Rabbits; D013574:Synaptosomes / Q000378:metabolism; D020866:omega-Conotoxin GVIA
D015220:Calcium Channels; D010455:Peptides; C053157:omega-conotoxin receptor; D020866:omega-Conotoxin GVIA; D002118:Calcium