Clinical application of a rapid, functional assay for multidrug resistance based on accumulation of the fluorescent dye, fluo-3.
A rapid and simple functional assay for P-glycoprotein (Pgp) using flow cytometry to measure the accumulation of the flurophore fluo-3 has been applied to samples from patients with B-cell chronic lymphocytic leukaemia (B-CLL). Peripheral blood lymphocytes from 37 patients with B-CLL were studied for Pgp. Pgp expression, using MRK-16, a monoclonal antibody recognising an external surface epitope of Pgp, was detected in 92% of patients with B-CLL. The functional assays for Pgp expression were positive in 78 and 59% of patients using the fluo-3 and doxorubicin (dox) assays, respectively. When compared with the MRK-16 assay, the fluo-3 assay had a sensitivity of 82% compared to a sensitivity of 56% for the dox assay (P = 0.004). The specificity of the fluo-3 and dox assays could not be evaluated because of the low number of MRK-16 negative CLL cells.
Wall
D M
DM
Department of Haematology, Heidelberg Repatriation Hospital.
Sparrow
R
R
Hu
X F
XF
Nadalin
G
G
Zalcberg
J R
JR
Marschner
I C
IC
Van der Weyden
M
M
Parkin
J D
JD
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000368:Aged; D000369:Aged, 80 and over; D000814:Aniline Compounds / Q000378:metabolism*; D002352:Carrier Proteins / Q000032:analysis*; D004317:Doxorubicin / Q000378:metabolism; D004351:Drug Resistance; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D012680:Sensitivity and Specificity; D014966:Xanthenes / Q000378:metabolism*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000814:Aniline Compounds; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D014966:Xanthenes; C059715:Fluo-3; D004317:Doxorubicin