Neurotrophic effects of annexin V on cultured neurons from embryonic rat brain.
Recombinant human annexin V showed survival promoting activity in embryonic rat neocortical and mesencephalic neurons in vitro. The neurotrophic effect was observed from a relatively low dose and in a dose-dependent manner. The neurotrophic activity of annexin V was completely blocked by anti-annexin V antibody. Northern blot analysis demonstrated that annexin V mRNA is expressed in non-neuronal cells in the CNS. These results suggest that annexin V plays certain roles in the CNS as a paracrine-type neurotrophic factor.
Takei
N
N
Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan.
Ohsawa
K
K
Imai
Y
Y
Nakao
H
H
Iwasaki
A
A
Kohsaka
S
S
D000595:Amino Acid Sequence; D000818:Animals; D017304:Annexin A5 / Q000276:immunology / Q000494:pharmacology*; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
D017304:Annexin A5; D012333:RNA, Messenger; D011994:Recombinant Proteins