title: Characteristics of [3H]glutamate binding sites in rat cerebellum.
abstract: [3H]glutamate binding has been studied using the synaptic membrane preparations of rat cerebellum. The binding was observed to be specific, saturable and reversible. Curvileniarity of the Scatchard plots revealed the presence of two distinct populations of binding sites (one with a high affinity and the other with a low affinity to glutamate). [3H]glutamate bound to cerebellar synaptic membranes was displaced by kainate, N-methyl-D-aspartate and quisqualate indicating the presence of these three subtypes of glutamate receptors in cerebellum.
authors.affiliation: Laboratory of Neurochemistry, Andre-Viallet Clinical Research Center, Hopital Saint-Luc, Montreal, Canada.
authors.forename: V L
authors.initials: VL
authors.lastname: Rao
authors.forename: C R
authors.initials: CR
authors.lastname: Murthy
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002531:Cerebellum / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D007608:Kainic Acid / Q000378:metabolism; D008297:Male; D016202:N-Methylaspartate / Q000378:metabolism; D016318:Quisqualic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000378:metabolism*; D013045:Species Specificity; D013570:Synaptic Membranes / Q000378:metabolism*
chemical_list: D005971:Glutamates; D017470:Receptors, Glutamate; D018698:Glutamic Acid; D016202:N-Methylaspartate; D016318:Quisqualic Acid; D007608:Kainic Acid