Nerve growth factor with insular cortical grafts induces recovery of learning and reestablishes graft choline acetyltransferase activity.
Rats showing disrupted taste aversion due to insular cortex (IC)-lesions received either IC-grafts with NGF, grafts without NGF, or NGF alone. An additional group served as lesioned controls. Only those animals that received IC-grafts with NGF recovered the ability to learn the conditioned taste aversion task, at 15 days post-graft. Choline acetyltransferase (ChAT) activity in the IC-grafts with, but not without NGF, was similar to the IC activity of unoperated controls. In contrast, glutamate decarboxylase activity was similar in all the groups. These findings suggest that IC-grafts associated with NGF induce recovery of learning abilities in IC-lesioned rats, which correlates with reestablishment of ChAT activity in the grafts at 15 days post-implantation.
Escobar
M L
ML
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México.
Jiménez
N
N
López-García
J C
JC
Tapia
R
R
Bermúdez-Rattoni
F
F
D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D016380:Brain Tissue Transplantation; D002540:Cerebral Cortex / Q000196:embryology / Q000293:injuries / Q000503:physiopathology / Q000637:transplantation*; D002795:Choline O-Acetyltransferase / Q000096:biosynthesis*; D016332:Fetal Tissue Transplantation; D006085:Graft Survival; D018021:Lithium Chloride; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology / Q000627:therapeutic use*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013649:Taste / Q000502:physiology*
D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D002795:Choline O-Acetyltransferase; D018021:Lithium Chloride