Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus.
A plasmid vector, termed pSG5rab.gp, that expresses the rabies virus glycoprotein under the control of an SV40 early promoter, was tested in C3H/He mice for induction of rabies virus-specific immune responses. Mice immunized intramuscularly with the pSG5rab.gp vector developed rabies virus glycoprotein-specific cytolytic T cells, lymphokine-secreting T helper cells of the TH1 subset, and rabies virus-neutralizing antibodies. Mice vaccinated with the pSG5rab.gp vector were fully protected against a subsequent challenge with rabies virus.
Xiang
Z Q
ZQ
Wistar Institute, Philadelphia, Pennsylvania 19104.
Spitalnik
S
S
Tran
M
M
Wunner
W H
WH
Cheng
J
J
Ertl
H C
HC
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D005260:Female; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010957:Plasmids / Q000235:genetics* / Q000276:immunology; D011818:Rabies / Q000517:prevention & control*; D011819:Rabies Vaccines / Q000235:genetics* / Q000276:immunology; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology
D000956:Antigens, Viral; D006023:Glycoproteins; D011819:Rabies Vaccines; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus