Autoantibodies to cytochrome P450 enzymes P450scc, P450c17, and P450c21 in autoimmune polyglandular disease types I and II and in isolated Addison's disease.
Patients with idiopathic Addison's disease have autoantibodies reacting with adrenal cortex. If Addison's disease is associated with other endocrine immune diseases like autoimmune polyglandular diseases (APD) type I and type II, antibodies may recognize all steroid-producing cells. We showed previously that one antigen recognized by APD-I sera is the cytochrome P450c17 hydroxylase. We have now looked for antibodies to P450c17 and to two other key enzymes in the steroid biosynthetic pathway, the P450scc and P450c21, in a series of patients with isolated Addison's disease (8 patients) or with APD-I or APD-II (50 and 9 patients, respectively). The result of antienzyme antibodies were further correlated with the immunofluorescence pattern against adrenal gland, testis, ovary, and placenta, and with the clinical findings presented. In APD-I patients with Addison's disease and in APD-II patients, antibodies to at least one of the P450 enzymes were frequently found (positive findings in 81% and 78%, respectively). Such antibodies were less frequent in APD-I patients without Addison's disease (21%) and in the isolated Addison cases (25%). In APD-I, antibodies recognized as frequently P450c17 and P450scc, specific for all steroid-producing cells as the adrenal specific enzyme P450c21. In contrast, patients with APD-II or with the isolated Addison's disease reacted almost exclusively with P450c21. Immunofluorescence studies showed good correlation with the known fact that the zona glomerulosa of the adrenal cortex is devoid of the P450c17, that the Leydig cells of the testis and the theca interna cells of the ovary express P450c17 and P450scc, and that the placental trophoblasts express only P450scc. The presence of antibodies to P450scc or to at least one of the tested P450 enzymes correlated significantly to gonadal failure in the females but not in the males.
Uibo
R
R
Institute of Biomedical Sciences, University of Tampere, Finland.
Aavik
E
E
Peterson
P
P
Perheentupa
J
J
Aranko
S
S
Pelkonen
R
R
Krohn
K J
KJ
D000224:Addison Disease / Q000201:enzymology* / Q000276:immunology; D000302:Adrenal Cortex / Q000737:chemistry / Q000201:enzymology; D000328:Adult; D000446:Aldehyde-Lyases / Q000032:analysis / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D002648:Child; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000032:analysis / Q000276:immunology*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D008297:Male; D010053:Ovary / Q000737:chemistry / Q000201:enzymology; D010920:Placenta / Q000737:chemistry / Q000201:enzymology; D016884:Polyendocrinopathies, Autoimmune / Q000201:enzymology* / Q000276:immunology; D011247:Pregnancy; D013254:Steroid 17-alpha-Hydroxylase; D013255:Steroid 21-Hydroxylase / Q000032:analysis / Q000276:immunology*; D013737:Testis / Q000737:chemistry / Q000201:enzymology
D001323:Autoantibodies; D003577:Cytochrome P-450 Enzyme System; D013255:Steroid 21-Hydroxylase; D013254:Steroid 17-alpha-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme; D000446:Aldehyde-Lyases