Preliminary crystallographic studies of the ligand-binding domain of the thyroid hormone receptor complexed with triiodothyronine.
A truncated, recombinant form of the thyroid hormone receptor, including the hormone binding domain, has been co-crystallized with the hormone T3. The crystals are monoclinic, most likely space group P2, with two molecules per asymmetric unit and cell dimensions a = 63.6 A, b = 80.8 A, c = 100.9 A and beta = 92.1 degrees. The crystals diffract to only medium resolution and decay rapidly in the X-ray beam using laboratory sources. By contrast, high resolution, high-quality data are obtained using synchrotron radiation in conjunction with cryocrystallography.
McGrath
M E
ME
Department of Biochemistry and Biophysics, University of California San Francisco 94143.
Wagner
R L
RL
Apriletti
J W
JW
West
B L
BL
Ramalingam
V
V
Baxter
J D
JD
Fletterick
R J
RJ
D001665:Binding Sites; D003461:Crystallography; D011988:Receptors, Thyroid Hormone / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D014284:Triiodothyronine / Q000737:chemistry*
D011988:Receptors, Thyroid Hormone; D011994:Recombinant Proteins; D014284:Triiodothyronine