Interactive potential of omega-3 fatty acids with clofibrate or DEHP on hepatic peroxisome proliferation in male Wistar rats.
The interactive potential of three known peroxisome proliferators, omega-3 fatty acids, clofibrate and di(2-ethylhexylphthalate (DEHP), was evaluated in male weanling Wistar rats for the effect on peroxisomal beta-oxidation. Omega-3 fatty acids were supplied by menhaden oil which was fed in six regimens: low fat (5% w/w), low fat and clofibrate (0.3% w/w) or DEHP (0.25% w/w), high fat (20% w/w), high fat and clofibrate or DEHP in the aforementioned concentrations. Induction of peroxisomal beta-oxidation was measured by changes in liver-to-body weight ratio, fatty acyl-CoA oxidase (FAO) activity, and peroxisomal bifunctional enzyme (PBE) quantity. Analysis of transformed data indicated a less than additive response in FAO activity with no deviation from additivity seen with liver-to-body ratios and PBE.
Wysynski
A M
AM
Environmental Health Sciences Program, School of Public Health, University of Massachusetts, Amherst 01003.
Baldwin
L A
LA
Leonard
D A
DA
Calabrese
E J
EJ
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D042962:Acyl-CoA Oxidase; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002994:Clofibrate / Q000494:pharmacology*; D004041:Dietary Fats / Q000008:administration & dosage*; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D007535:Isomerases / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008830:Microbodies / Q000187:drug effects* / Q000201:enzymology; D009097:Multienzyme Complexes / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D063994:Peroxisomal Bifunctional Enzyme; D051381:Rats; D017208:Rats, Wistar
D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D009097:Multienzyme Complexes; D004051:Diethylhexyl Phthalate; D010088:Oxidoreductases; D015094:3-Hydroxyacyl CoA Dehydrogenases; D042962:Acyl-CoA Oxidase; D004746:Enoyl-CoA Hydratase; D063994:Peroxisomal Bifunctional Enzyme; D007535:Isomerases; D002994:Clofibrate