Import of the mammalian cytochrome P450 (scc) precursor into plant mitochondria.
According to previous reports (Matocha M. F. and Waterman M. R. (1984) J. Biol. Chem. 259, 8672-8678 and (1986) Arch. Biochem. Biophys. 250, 456-460) import of the cytochrome P450 (scc) precursor into mitochondria is tissue-specific. The present paper shows that in vitro synthesized bovine cytochrome P450 (scc) precursor can be imported into isolated soybean cotyledon mitochondria and processed therein to the mature size product. This shows that heterologous import of the cytochrome P450 (scc) precursor is possible and that import into mitochondria of this precursor is not restricted to steroidogenic tissues.
Luzikov
V N
VN
Belozersky Institute of Physico-chemical Biology, Moscow State University, Russia.
Novikova
L A
LA
Whelan
J
J
Hugosson
M
M
Glaser
E
E
D000818:Animals; D001692:Biological Transport; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D013025:Glycine max; D013045:Species Specificity
D011498:Protein Precursors; D002786:Cholesterol Side-Chain Cleavage Enzyme