Analysis of L-dopa in pharmaceutical preparations and of total phenols content in urine by means of an enzyme-amperometric sensor.
An enzyme-amperometric method is proposed for the analysis of total phenols and L-dopa; the method is based on the enzyme tyrosinase, which is immobilized in a Nylon membrane and coupled to an oxygen gas-diffusion amperometric electrode. The method was applied to the determination of total phenols in urine and to L-dopa in formulations and was evaluated as a promising alternative to currently adopted methods, e.g. to a classical spectrophotometric technique, chosen as a reference method.
Campanella
L
L
Chemistry Department, University La Sapienza, Roma, Italy.
Beone
T
T
Sammartino
M P
MP
Tomassetti
M
M
D015374:Biosensing Techniques; D004800:Enzymes, Immobilized; D006801:Humans; D007980:Levodopa / Q000032:analysis*; D008567:Membranes, Artificial; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry; D010636:Phenols / Q000652:urine*
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009757:Nylons; D010636:Phenols; D007980:Levodopa; D014442:Monophenol Monooxygenase