title: Relationships between cell surface protease and acid phosphatase activities of Leishmania promastigote.
abstract: A correlation between the ratio of the cell surface protease activity to phosphatase activity and the complexity of the pattern of cell surface exposed polypeptides of Leishmania promastigotes was demonstrated for various strains grown under similar conditions. The ratio of the cell surface protease activity to acid phosphatase activity was high for L. major and L.b. panamensis and it correlates with the expression of a single polypeptide of 63 KDa on their cell surface. Intermediate and lower ratios of these enzymatic activities relate with more complex radio-iodinated patterns: two main bands in L.b. guyanensis (70 and 58 KDa) and L.b. braziliensis (72 and 60 KDa) and three main bands 65, 50, 27 KDa in all L.m. mexicana strains tested. Evidence is presented that the acid phosphatase located on the L.m. mexicana cell surface is not an artifact due to a secondary absorption of the secreted acid phosphatase from the culture medium. These results confirm the Leishmania antigen cell surface heterogeneity. The implications on the biology of Leishmania and the clinical manifestation of leishmaniasis are discussed.
authors.affiliation: Departamento de Biologia Celular, Facultad de Ciencias, Universidad Central de Venezuela, Caracas.
authors.forename: A G
authors.initials: AG
authors.lastname: Hernandez
authors.forename: A
authors.initials: A
authors.lastname: Rascon
authors.forename: S
authors.initials: S
authors.lastname: Kutner
authors.forename: H
authors.initials: H
authors.lastname: Roman
authors.forename: Z
authors.initials: Z
authors.lastname: Campos
mesh_terms: D000135:Acid Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D002462:Cell Membrane / Q000378:metabolism; D010450:Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007891:Leishmania / Q000201:enzymology*; D007892:Leishmania braziliensis / Q000378:metabolism; D018334:Leishmania guyanensis / Q000378:metabolism; D018320:Leishmania major / Q000378:metabolism; D007894:Leishmania mexicana / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000302:isolation & purification / Q000378:metabolism; D013045:Species Specificity
chemical_list: D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D015800:Protozoan Proteins; D000135:Acid Phosphatase; D010450:Endopeptidases