Ultrastructure and cytochemical characteristics of leukocyte infected with Hepatozoon canis.
We have observed the ultrastructure of a canine leukocyte infected with Hepatozoon canis by transmission electron microscope. The infected leukocytes contained many small vesicles and a few typical granules in the cytoplasm. They also contained a few rough surfaced endoplasmic reticula, mitochondria and a segmented nucleus with condensed chromatin. Cytochemical analysis indicated that the infected leukocytes with H. canis contained little alkaline phosphatase, peroxidase, naphthol AS-D chloroacetate esterase or alpha naphthol acetate esterase. These results suggested that the infected leukocytes were neutrophils or monocytes which lost their typical characteristics.
Murata
T
T
Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University, Japan.
Inoue
M
M
Kanoe
M
M
Kono
Y
Y
Ishida
M
M
Horio
M
M
Shimada
M
M
Yokoyama
M
M
Taura
Y
Y
Nakama
S
S
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D004283:Dog Diseases; D004285:Dogs; D004950:Esterases / Q000097:blood; D016783:Eucoccidiida / Q000302:isolation & purification*; D007962:Leukocytes / Q000469:parasitology* / Q000473:pathology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009000:Monocytes / Q000201:enzymology / Q000473:pathology; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology; D011529:Protozoan Infections, Animal / Q000097:blood*
D004950:Esterases; D000469:Alkaline Phosphatase