Monoclonal antibodies to a zinc-binding protein of rat Paneth cells.
Paneth cells are morphologically well characterized but their function has been not elucidated. Previously, we identified and purified a 90 KD zinc-binding protein (ZBPP-1) in rat intestine that was localized to Paneth cell granules, consistent with their high zinc content. To further elucidate the structure and function of ZBPP-1, we immunized Balb/c mice with purified ZBPP-1 and identified four independent monoclonal antibodies (MAb) producing MAb ZIP-1 (IgM), ZIP-2 (IgG1), ZIP-3 (IgM), and ZIP-4 (IgM). Immunohistochemistry (IHC) and immunoelectron microscopy (IEM) with these MAb showed positive staining of Paneth cell cytoplasmic granules. MAb ZBPP-1 also stained a population of mononuclear cells in the lamina propria of digestive tract mucosa and a few cells in spleen, presumably a subset of macrophages. These MAb will provide a useful tool to study the function of Paneth cells in human health and disease, since they cross-reacted with human intestinal Paneth cells and mucosal mononuclear cells.
Sawada
M
M
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
Horiguchi
Y
Y
Abujiang
P
P
Miyake
N
N
Kitamura
Y
Y
Midorikawa
O
O
Hiai
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification*; D003594:Cytoplasmic Granules / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar; D015032:Zinc / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; C017800:zinc-binding protein; D015032:Zinc