Lipid peroxidation in isolated membranes of cerebral cortex, heart and kidney.
The extent of ADP.Fe/NADPH-induced lipid peroxidation measured as production of thiobarbituric acid-reactive substances (TBARS) was determined in isolated membranes from cerebral cortex, heart and kidney of 21-days-old rats. The time course of lipid peroxidation showed higher production of TBARS in cerebral cortex than in heart and kidney. Our data indicate that high level of TBARS production is not due to high activity of NADPH oxidoreductase but due to high content of endogenous lipids in cerebral cortex membranes that could be modified. Higher production of TBARS in cerebral cortex is the result of higher content of lipids in cerebral cortex membranes because NADPH cytochrome c reductase activity in membranes of cerebral cortex is lower than that of heart and kidney.
Rauchová
H
H
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.
Kalous
M
M
Drahota
Z
Z
Koudelová
J
J
Mourek
J
J
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D005260:Female; D007501:Iron / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D009249:NADP; D000244:Adenosine Diphosphate; D007501:Iron; D009251:NADPH-Ferrihemoprotein Reductase