A membrane guanylate cyclase Drosophila homolog gene exhibits maternal and zygotic expression.
A putative membrane form of guanylate cyclase gene was identified in region 32 of the second chromosome of Drosophila melanogaster. The Drosophila protein has a single hydrophobic sequence that divides the protein into a putative extracellular region and a cytoplasmic catalytic region which contains tyrosine kinase and cyclase domains showing varying degrees of conservation with other guanylate and adenylate cyclases. The gene shows a very interrupted organization with small exons separated by small introns and a low level of expression. Transcripts have been localized in situ during oogenesis in the germarium and later in stages 10-14 of egg chamber development. The putative maternal transcript can be seen in very early embryos and reappears later as a product of zygotic gene expression.
Gigliotti
S
S
Istituto Internazionale di Genetica e Biofisica, CNR, Naples, Italy.
Cavaliere
V
V
Manzi
A
A
Tino
A
A
Graziani
F
F
Malva
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000201:enzymology; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression; D006162:Guanylate Cyclase / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D015053:Zygote / Q000378:metabolism*
D018076:DNA, Complementary; D006162:Guanylate Cyclase