Method for detection of picornavirus capsid binders with soluble intercellular adhesion molecule 1.
A method utilizing immobilized major group rhinoviruses and biotinylated soluble intercellular adhesion molecule 1 has been developed for the detection of capsid binders. Binding measurements indicate a loss in relative affinity of biotinylated soluble intercellular adhesion molecule 1 for rhinoviruses by capsid binders. This method and a new class of capsid binders are discussed.
Last-Barney
K
K
Department of Immunology, Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, Connecticut 06877.
Marlin
S D
SD
Pal
K
K
Cahill-Feehan
C
C
McNally
E J
EJ
Jeanfavre
D D
DD
Merluzzi
V J
VJ
D001710:Biotin / Q000378:metabolism; D002213:Capsid / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006367:HeLa Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D010849:Picornaviridae / Q000378:metabolism*; D012229:Rhinovirus / Q000378:metabolism*; D012995:Solubility; D013844:Thiazoles / Q000032:analysis* / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D013844:Thiazoles; D018799:Intercellular Adhesion Molecule-1; D001710:Biotin