title: Characterization of filoviruses based on differences in structure and antigenicity of the virion glycoprotein.
abstract: Eight different filovirus isolates, representing major episodes of filovirus hemorrhagic disease, were propagated for structural and antigenetic analyses of their glycoprotein (GP). Carbohydrate analysis revealed that N- and O-glycosylation are features of filovirus GPs. Oligosaccharide side chains differed in their sialylation pattern and seemed to be cell line-dependent. Marburg virus (MBG) isolates are clearly distinguished from Ebola (EBO) and Reston viruses by a lack of terminal sialic acids when propagated in E6 and MA-104 cells. It was also determined that GP-specific antisera failed to show any cross-reactivity between MBG isolates and other filoviruses. These data, together with prior findings, indicate that the genus Filovirus can be divided into a MBG group and EBO group.
authors.affiliation: Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
authors.forename: H
authors.initials: H
authors.lastname: Feldmann
authors.forename: S T
authors.initials: ST
authors.lastname: Nichol
authors.forename: H D
authors.initials: HD
authors.lastname: Klenk
authors.forename: C J
authors.initials: CJ
authors.lastname: Peters
authors.forename: A
authors.initials: A
authors.lastname: Sanchez
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D016563:Filoviridae / Q000737:chemistry* / Q000276:immunology; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D014709:Vero Cells; D014771:Virion / Q000737:chemistry* / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins