Characteristic localization of alpha 1- and alpha 2-adrenoceptors in the human kidney.
1. The localization of alpha-adrenoceptors in the plasma membranes of human kidney were investigated by radioligand binding, using an alpha 1-antagonist, [3H]-bunazosin, and an alpha 2-antagonist, [3H]-rauwolscine. 2. Both the maximum binding (Bmax) and dissociation constant (Kd) of [3H]-bunazosin were greater in the cortex than in the medulla. The Bmax of [3H]-rauwolscine in the medulla was greater than in the cortex. 3. Thus, alpha 1-adrenoceptors appeared to be localized predominantly in the cortex, while the alpha 2-adrenoceptors were mainly present in the medulla of the human kidney.
Minamisawa
K
K
Second Department of Internal Medicine, Yokohama City University of Medicine, Japan.
Umemura
S
S
Hirawa
N
N
Hayashi
S
S
Toya
Y
Y
Ishikawa
Y
Y
Yasuda
G
G
Ishii
M
M
D000317:Adrenergic alpha-Antagonists / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001665:Binding Sites; D002462:Cell Membrane / Q000737:chemistry; D005260:Female; D006801:Humans; D007672:Kidney Cortex / Q000737:chemistry* / Q000378:metabolism; D007679:Kidney Medulla / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011799:Quinazolines / Q000378:metabolism; D011869:Radioligand Assay; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis* / Q000378:metabolism; D018341:Receptors, Adrenergic, alpha-2 / Q000032:analysis* / Q000378:metabolism; D015016:Yohimbine / Q000378:metabolism
D000317:Adrenergic alpha-Antagonists; D011799:Quinazolines; D018340:Receptors, Adrenergic, alpha-1; D018341:Receptors, Adrenergic, alpha-2; D015016:Yohimbine; C018176:bunazosin