Stimulation of dopamine biosynthesis in cultured PC 12 phaeochromocytoma cells by the coenzyme nicotinamide adeninedinucleotide (NADH).
The activity of the tyrosine hydroxylase, the enzyme which is diminished in the brains of Parkinson patients, has been measured in cultured PC 12 rat phaeochromocytoma cells. In the same way dopamine content in the medium after incubating these cells with or without NADH was assayed. The experiment shows that NADH is able to increase the activity of the tyrosine hydroxylase and dopamine - production in PC 12 cells up to 6 times. The results provide evidence that NADH is able to stimulate dopamine - biosynthesis directly.
Vrecko
K
K
Institute for Medical Chemistry, University of Graz, Austria.
Birkmayer
J G
JG
Krainz
J
J
D000818:Animals; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D004298:Dopamine / Q000096:biosynthesis*; D006834:Hydrazines / Q000494:pharmacology; D009243:NAD / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D013268:Stimulation, Chemical; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D006834:Hydrazines; D009243:NAD; C004297:3-hydroxybenzylhydrazine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine