Identification of a sweet potato feathery mottle virus isolate from China (SPFMV-CH) by the polymerase chain reaction with degenerate primers.
Four degenerate oligonucleotide primers derived from conserved regions of the genome of potyviruses have been designed. A combined assay of reverse transcription and the polymerase chain reaction utilizing these primers on total RNA extracted from Ipomoea purpurea infected with a sweet potato feathery mottle virus isolate from China (SPFMV-CH), amplified a 1.35 kb and a 830 bp fragment. These amplified fragments were cloned into the Bluescript plasmid and sequenced. The comparison of the sequence of the N-terminal part of the coat protein of SPFMV-CH with those published for two other strains of SPFMV, showed a strong relationship between SPMV-CH and -RC.
Colinet
D
D
Faculté des Sciences Agronomiques de l'Etat, Laboratoire de Pathologie Végétale, Gembloux, Belgium.
Kummert
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D002681:China; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014773:Virology / Q000379:methods
D017931:DNA Primers; D004279:DNA, Viral