Characterizations of sea urchin fibrillar collagen and its cDNA clone.
Collagens were isolated from the adult test of the sea urchin species, Hemicentrotus pulcherrimus and Strongylocentrotus purpuratus, and their molecular properties were compared with those of Asthenosoma ijimai collagen. Collagens from H. pulcherrimus and S. purpuratus comprised two major alpha-chains (alpha 120 and alpha 90) and a minor chain (alpha 140), while collagen from A. ijimai contained four alpha-chains (alpha 1, alpha 2, alpha 3 and alpha 4). Based on their molecular and immunological properties, the alpha 90 chain of H. pulcherrimus and S. purpuratus, and the alpha 2 and alpha 4 chains of A. ijimai are grouped together, while the alpha 120 and alpha 140 chains of H. pulcherrimus and S. purpuratus, and the alpha 1 and alpha 3 chains of A. ijimai are classified into another group. It is likely that collagen molecules of sea urchins are heterotrimers composed of these two types of alpha-chains. A cDNA of collagen was cloned from the cDNA library prepared from mRNA of H. pulcherrimus test and denoted as Hpcol1. This clone contained sequences for uninterrupted triple helical domain (378 amino acids), carboxyl telopeptide (28 amino acids) and carboxyl propeptide (225 amino acids). This structure is characteristic for fibril-forming collagens and was shown to encode alpha 120 and alpha 140 chains of H. pulcherrimus collagen. Hpcol1-mRNA was expressed in embryos as early as the prism stage.
Tomita
M
M
Department of Biology, Faculty of Science, Tokyo Metropolitan University, Japan.
Kinoshita
T
T
Izumi
S
S
Tomino
S
S
Yoshizato
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003094:Collagen / Q000235:genetics* / Q000302:isolation & purification; D018076:DNA, Complementary / Q000032:analysis* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011347:Procollagen / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012617:Sea Urchins / Q000196:embryology / Q000235:genetics*; D016415:Sequence Alignment
D018076:DNA, Complementary; D011347:Procollagen; D012333:RNA, Messenger; D003094:Collagen