Insertion sequence IS6100 on plasmid pOAD2, which degrades nylon oligomers.
The nucleotide sequence of repeated sequence I, which appears in five regions on nylon oligomer-degrading plasmid pOAD2, harbored in Flavobacterium sp. strain K172, was determined. The five regions of repeated sequence I had 880 bp of identical sequence, and the sequence was identical to that of IS6100, an insertion sequence classified in the IS6 family, initially found in Mycobacterium fortuitum. Sequences homologous to that of IS6100 were found for another nylon oligomer-degrading plasmid, pNAD2, harbored in Pseudomonas sp. strain NK87, by Southern hybridization experiments.
Kato
K
K
Department of Biotechnology, Osaka University, Japan.
Ohtsuki
K
K
Mitsuda
H
H
Yomo
T
T
Negoro
S
S
Urabe
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000145:classification; D005417:Flavobacterium / Q000235:genetics*; D008969:Molecular Sequence Data; D009757:Nylons / Q000378:metabolism*; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009757:Nylons