Effect of growth hormone and insulin-like growth factor-I on immunoglobulin production by and growth of human B cells.
The effect of human GH and insulin-like growth factor-I (IGF-I) on immunoglobulin (Ig) production by and proliferation of human B cells was studied in serum- and hormone-free medium. GH enhanced the production of Ig by and thymidine uptake of the human lymphoblastoid B cell lines, CBL and GM-1056. IGF-I, but not IGF-II or insulin, also enhanced Ig production by and proliferation of CBL and GM-1056. However, the GH-induced enhancement was not mediated by IGF-I inasmuch as enhancement was blocked by anti-GH antibody but not by anti-IGF-I antibody or anti-IGF-I receptor antibody. Conversely, the IGF-I-induced enhancement was blocked by either anti-IGF-I antibody or anti-IGF-I receptor antibody but not by anti-GH antibody. GH and IGF-I also enhanced Ig production by and proliferation of the human lymphoblastoid B cell lines, CESS, GM-1500, SKW, and GM-3332. Furthermore, GH and IGF-I enhanced production of IgG1, IgG2, IgG3, IgG4, IgA1 IgA2, and IgM by and thymidine uptake of tonsillar B cells stimulated with Staphylococcus aureus Cowan strain I. Again, the GH-induced enhancement was blocked by anti-GH antibody whereas the IGF-I-induced enhancement was blocked by either anti-IGF-I antibody or anti-IGF-I receptor antibody but not vice versa.
Kimata
H
H
Department of Pediatrics, Kyoto University Hospital, Japan.
Yoshida
A
A
D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D002455:Cell Division; D002460:Cell Line; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D007700:Kinetics; D014066:Palatine Tonsil / Q000166:cytology; D010455:Peptides / Q000494:pharmacology
D007136:Immunoglobulins; D010455:Peptides; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D013006:Growth Hormone