The capacity of central and peripheral catecholaminergic neurons to innervate the pineal organ and cerebral cortex of the rat: in vitro immunohistochemical observations.
The locus coeruleus (LC) or superior cervical ganglion (SCG) of neonatal rats were co-cultured either with the pineal organ or cerebral cortex (CX) to investigate the innervating capacity of central and peripheral catecholamine neurons under these experimental conditions. After 2 weeks of co-culturing, cultures were fixed for tyrosine hydroxylase (TH) immunohistochemistry to examine the distribution of catecholamine neurons and their fibers. Glial fibrillary acidic protein and fibronectin immunohistochemistry was performed to determine the cell types proliferating around the explants. In LC/CX co-cultures, numerous astrocytes spread between the two explants, and TH-immunoreactive neurites were generally seen to invade CX explants. In contrast, neurite extension from LC to pineal explants occurred only when a glial cell sheet grew between the two explants, and when the pineal explants were not surrounded by a tight fibronectin-positive cell layer. Neurites of the SCG usually invaded both CX and pineal explants, regardless of the existence of glial or non-glial cell layer. These results indicate that central and peripheral catecholamine neurites have the potential of invading both the cortex and pineal, although they are distributed only in particular regions of the intact brain. The distribution of LC neurites, however, seems to be profoundly affected by the cell types spreading around the explants; glial cells appear to support LC neurite extension, whereas non-glial cells appear to inhibit it.
Nonaka
T
T
Department of Surgical Neurology, Jichi Medical School, Tochigi, Japan.
Araki
M
M
Kimura
H
H
Nagatsu
I
I
Satoh
F
F
Masuzawa
T
T
D000818:Animals; D000831:Animals, Newborn; D002395:Catecholamines / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D046508:Culture Techniques; D005353:Fibronectins / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D008125:Locus Coeruleus / Q000166:cytology / Q000378:metabolism; D016501:Neurites / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000378:metabolism*; D010870:Pineal Gland / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017783:Superior Cervical Ganglion / Q000166:cytology / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D002395:Catecholamines; D005353:Fibronectins; D005904:Glial Fibrillary Acidic Protein; D014446:Tyrosine 3-Monooxygenase