Sequences of the N and M genes of the sigma virus of Drosophila and evolutionary comparison.
The genome of the sigma rhabdovirus of Drosophila melanogaster consists of six genes in the order 3' N-2-3-4-G-L 5'. The nucleotide sequences of the N and of the fourth genes were determined from cDNA clones. Each gene contained a single long open reading frame encoding polypeptides with predicted MW of 50 and 25 kDa, respectively. Evidence that these genes encode the nucleocapsid N and the matrix M proteins were obtained using antibodies raised against recombinant proteins derived from the cloned genes and expressed in Escherichia coli. The M and N predicted amino acid sequences were compared with those of other rhabdoviruses. The M protein of sigma virus shared a similar domain arrangement to the other M proteins, but it showed very little sequence conservation. The N protein of sigma virus showed no significant homology with its counterpart in SYNV or IHNV and VSHV; it did, however, show sequence homology with the N of four vesiculoviruses and two lyssaviruses. The extent of amino acid identity suggests that sigma virus occupies an intermediate evolutionary position between these two genera. Some conserved motifs in the M and N proteins were deduced from the comparisons.
Bras
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Laboratoire de Génétique des Virus, CNRS, Gif-sur-Yvette, France.
Teninges
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Dezelee
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002213:Capsid / Q000145:classification / Q000235:genetics* / Q000276:immunology; D003429:Cross Reactions; D018076:DNA, Complementary / Q000235:genetics; D004331:Drosophila melanogaster / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012209:Rhabdoviridae / Q000145:classification / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014758:Viral Core Proteins / Q000145:classification / Q000235:genetics* / Q000276:immunology; D014763:Viral Matrix Proteins / Q000145:classification / Q000235:genetics* / Q000276:immunology; D014771:Virion / Q000235:genetics
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins