Chiral differentiation of the optical isomers of racemethorphan and racemorphan in urine by capillary zone electrophoresis.
A novel capillary zone electrophoretic method based on the use of propanol-modified beta-cyclodextrin buffers has been developed; it unambiguously detects and quantitates the optical isomers of racemethorphan and racemorphan without prior derivatization. Detection limits in urine down to 20 ppb have been obtained. The method uses a solid-phase extraction recovery of analytes followed by ultraviolet detection. Separation conditions include the use of 60mM beta-cyclodextrin and 50mM sodium dodecyl sulfate in a buffer containing 20% 1-propanol.
Aumatell
A
A
Australian Government Analytical Laboratories, Pymble, NSW.
Wells
R J
RJ
D003915:Dextromethorphan / Q000652:urine*; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D007981:Levorphanol / Q000652:urine*; D008297:Male; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D007981:Levorphanol; D003915:Dextromethorphan