Heterologous expression of endo-beta-1,4-D-glucanase from Clostridium cellulovorans in Clostridium acetobutylicum ATCC 824 following transformation of the engB gene.
Heterologous expression of the Clostridium cellulovorans engB gene by Clostridium acetobutylicum BKW-1 was detected as zones of hydrolysis on carboxymethyl cellulose (CMC) Trypticase glucose yeast plates stained with Congo red. The extracellular cellulase preparation from C. acetobutylicum BKW-1 has a specific activity towards CMC which is more than fourfold that present in C. acetobutylicum ATCC 824. Western blot (immunoblot) analysis using the C. cellulovorans anti-EngB primary antibody demonstrated that an additional 44-kDa protein band was present in the supernatant derived from C. acetobutylicum BKW-1 but was not present in ATCC 824 or ATCC 824(pMTL500E).
Kim
A Y
AY
Department of Food Science, University of Illinois, Urbana 61801.
Attwood
G T
GT
Holt
S M
SM
White
B A
BA
Blaschek
H P
HP
D001673:Biodegradation, Environmental; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002480:Cellulase / Q000235:genetics* / Q000276:immunology; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D005285:Fermentation; D015870:Gene Expression; D005798:Genes, Bacterial; D007120:Immunochemistry; D010957:Plasmids / Q000235:genetics; D014170:Transformation, Genetic
D002480:Cellulase; D002266:Carboxymethylcellulose Sodium