title: Laser Doppler flux and tissue oxygen tension as indicators of colonic perfusion in pigs.
abstract: OBJECTIVE
  To evaluate laser Doppler flowmetry (LDF) and tissue oximetry (TpO2) for intraoperative assessment of colonic perfusion in pigs.
  
  
  METHODS
  Eight pigs weighing from 19-22 kg.
  
  
  METHODS
  Relationships of laser Doppler flux and TpO2 to mean arterial pressure (MAP) were measured in porcine sigmoid colon. The influence of different fractional inspired oxygen concentrations (FiO2) and the variability of the laser Doppler flux were evaluated.
  
  
  RESULTS
  Highly significant reproducible non-linear perfusion compared with pressure relations were found with both methods (r > or = 0.9, p < 0.0001). There was a rapid decrease when MAP fell below 90 mmHg. The dependency of TpO2 on FiO2 was omitted by using the ratio TpO2:PaO2. The coefficient of variation of repeated LDF recordings of 30 second duration with the hand held probe was 0.11 (0.07-0.16) (median and 95% confidence interval). Three recordings gave a reproducible mean flux with an estimated precision of 10% either way. Simultaneous recordings with sutured and hand-held probes correlated well (r > or = 0.9, p < or = 0.01).
  
  
  CONCLUSIONS
  Both methods yielded quantitative and reproducible intraoperative assessments of colonic perfusion, and are applicable for clinical use.
authors.affiliation: Unit of Vascular Surgery, Aker Hospital, Oslo, Norway.
authors.forename: K
authors.initials: K
authors.lastname: Krohg-Sørensen
authors.forename: P D
authors.initials: PD
authors.lastname: Line
authors.forename: K
authors.initials: K
authors.lastname: Kvernebo
mesh_terms: D000818:Animals; D001794:Blood Pressure; D012809:Colon, Sigmoid / Q000098:blood supply* / Q000378:metabolism*; D017078:Laser-Doppler Flowmetry; D010100:Oxygen / Q000097:blood / Q000378:metabolism*; D012039:Regional Blood Flow; D013552:Swine
chemical_list: D010100:Oxygen