Serodiagnostic efficiency of phospholipid associated protein of Mycobacterium tuberculosis H37Rv.
The phospholipid-associated protein (55-67 kDa) fraction of Mycobacterium tuberculosis H37Rv was purified as the DE-V protein fraction. This DE-V fraction was used for diagnosis of tuberculosis by enzyme-linked immunosorbent assay (ELISA), detecting IgG antibody in sera collected from different categories of tuberculosis patients, i.e. with acid fast bacilli (AFB) culture-positive pulmonary tuberculosis, with AFB culture-negative, but radiologically suspected, pulmonary tuberculosis, extrapulmonary tuberculosis, and control groups of patients suffering from diseases other than tuberculosis (asthma and/or rhinitis, lepromatous leprosy) as well as from healthy volunteers. Encouraging operational ELISA validity could be achieved with 93% sensitivity, 100% specificity, 97% efficiency, 100% positive predictivity and 95% negative predictability even among the extrapulmonary and suspected pulmonary tuberculosis patients. The above assay was insensitive but with 100% specificity among control group of patients suffering from diseases other than tuberculosis. The DE-V protein fraction was associated with phosphatidyl inositol and phosphatidyl inositol mannosides. The dissociation of phospholipid-protein complex decreased ELISA specificity. ELISA reactivity of the DE-V fraction appeared to be thermostable; thus, it may have serodiagnostic utility in developing countries.
Kaushik
N K
NK
CSIR Centre for Biochemicals, Delhi University Campus, India.
Sharma
P
P
Shah
A
A
Venkitasubramanian
T A
TA
D000907:Antibodies, Bacterial / Q000032:analysis; D001249:Asthma / Q000175:diagnosis; D001426:Bacterial Proteins / Q000032:analysis*; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D015440:Leprosy, Lepromatous / Q000175:diagnosis; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010743:Phospholipids / Q000032:analysis*; D011237:Predictive Value of Tests; D012220:Rhinitis / Q000175:diagnosis; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D010743:Phospholipids