Detection of Salmonella spp. in oysters by PCR.
PCR DNA amplification of a region of the himA gene of Salmonella typhimurium specifically detected Salmonella spp. In oysters, 1 to 10 cells of Salmonella spp. were rapidly detected by the PCR following a pre-enrichment step to increase sensitivity and to ensure that detection was based on the presence of viable Salmonella spp.
Bej
A K
AK
Department of Biology, University of Alabama at Birmingham 35294.
Mahbubani
M H
MH
Boyce
M J
MJ
Atlas
R M
RM
D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial