Endogenous factor activating Na,K-ATPase induced by blockade of adrenoceptors.
It was established that the regulatory factor released from the effector cells under the blockade of postsynaptic alpha- and beta-adrenoceptors by phentolamine and propranolol (0.5-1.0 microM) and activating noradrenergic uptake and synthesis increased the activity of Na,K-ATPase of isolated rat organs and partially purified Na,K-ATPase isolated from the rat brain. The regulatory factor is a protein with molecular mass 25-100 kDa, adsorbed on phenylsepharose CL-4B and eluated with 30 mM KCl. It consists of two active components with pI 5.1 and 5.9 which in admixture (1:1) activate Na,K-ATPase by 60%.
Manukhin
B N
BN
Institute of Developmental Biology, Russian Academy of Sciences, Moscow.
Erokhov
P A
PA
Volina
E V
EV
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D007525:Isoelectric Focusing; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008970:Molecular Weight; D011199:Potentiometry; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000302:isolation & purification / Q000378:metabolism*; D014020:Tissue Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D000317:Adrenergic alpha-Antagonists; D014020:Tissue Extracts; D000254:Sodium-Potassium-Exchanging ATPase