title: Transglutaminase covalently incorporates amines into human immunodeficiency virus envelope glycoprotein gp120 in vitro.
abstract: Human immunodeficiency virus envelope glycoprotein gp120, but not its precursor gp160, covalently incorporates both spermidine and glycine ethyl ester in the presence of Ca2+ and transglutaminase purified from guinea pig liver. The examined ability to act as enzyme substrate of various glutamine-containing gp120 fragments, including the principal neutralizing determinant, the CD4 binding domain, and the sequence 254-274, suggested to be involved in post-binding events and in virus entry in the host cell, indicated the glutamine-265 as possible reactive acyl donor site of the protein.
authors.affiliation: Department of Biochemistry and Biophysics, University of Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Mariniello
authors.forename: C
authors.initials: C
authors.lastname: Esposito
authors.forename: V
authors.initials: V
authors.lastname: Gentile
authors.forename: R
authors.initials: R
authors.lastname: Porta
mesh_terms: D000586:Amination; D000595:Amino Acid Sequence; D000818:Animals; D015686:Gene Products, env / Q000378:metabolism*; D005998:Glycine / Q000031:analogs & derivatives / Q000378:metabolism; D006168:Guinea Pigs; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D019302:HIV Envelope Protein gp160; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D013095:Spermidine / Q000378:metabolism; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors; C022010:glycine ethyl ester; D011503:Transglutaminases; D005998:Glycine; D013095:Spermidine