The function of c-fms in hairy-cell leukemia: macrophage colony-stimulating factor stimulates hairy-cell movement.
Hairy cells (HCs) and some activated B cells express high levels of macrophage colony-stimulating factor (M-CSF) (CSF-1) receptor, but the functional effects of the cytokine on B cells have not been previously identified. Using video microscopy, image analysis, and migration assays, M-CSF was shown to induce chemokinetic and chemotactic movement of HCs. This movement response involved transition to a highly mobile, rounded cell form and was accompanied by distinctive changes in F-actin polymerization and distribution. Furthermore, the M-CSF-induced motility was substantially modified by the adhesive protein used as a substratum and involved qualitative changes in the function of the alpha v beta 3 integrin of HCs. It is suggested that the findings are relevant to the pathophysiology of hairy-cell leukemia (HCL) in particular, and to the biology of B-cell migration in general.
Burthem
J
J
Department of Haematology, University of Liverpool, UK.
Baker
P K
PK
Hunt
J A
JA
Cawley
J C
JC
D000199:Actins / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D016023:Integrins / Q000502:physiology; D007943:Leukemia, Hairy Cell / Q000473:pathology / Q000503:physiopathology*; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014741:Video Recording
D000199:Actins; D015815:Cell Adhesion Molecules; D016023:Integrins; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor