title: Comparison of the resistance to infection of intestinal submucosa arterial autografts versus polytetrafluoroethylene arterial prostheses in a dog model.
abstract: OBJECTIVE
  Prosthetic graft infection represents a most challenging complication to the vascular surgeon. Although expanded polytetrafluoroethylene (ePTFE) grafts have an acceptable patency rate, especially in the large-diameter arterial location, bacterial contamination of this material usually requires surgical removal of the graft.
  
  
  METHODS
  We compared the resistance of large-diameter ePTFE grafts and grafts constructed of small intestinal submucosa (SIS) to deliberate infection with Staphylococcus aureus. Eighteen dogs were divided into two equal groups, and the infrarenal aorta was replaced with either ePTFE or SIS graft material. One hundred million S. aureus organisms were deposited directly on the graft at the time of surgery, and the dogs were observed for 30 days.
  
  
  RESULTS
  One dog with an ePTFE graft died of hemorrhage from anastomosis site at 21 days. Of the remaining eight dogs with ePTFE grafts, four had positive culture results from the removed graft material, and all had histologic evidence for persistent infection. These dogs also had chronic fever, and the average white blood cell count at day 30 was 15,600/mm3. All nine dogs with SIS grafts had patent grafts, were afebrile after the first week, had an average white blood cell count of 11,500/mm3 at 30 days (p value = NS), had negative culture results, and had the histologic appearance of graft remodeling with collagen that was free of active inflammation.
  
  
  CONCLUSIONS
  We conclude that large-diameter arterial SIS grafts are more resistant to persistent infection with S. aureus than ePTFE grafts in this dog model of deliberate bacterial inoculation.
authors.affiliation: Hillenbrand Biomedical Engineering Center, Purdue University, W. Lafayette, IN 47907.
authors.forename: S F
authors.initials: SF
authors.lastname: Badylak
authors.forename: A C
authors.initials: AC
authors.lastname: Coffey
authors.forename: G C
authors.initials: GC
authors.lastname: Lantz
authors.forename: W A
authors.initials: WA
authors.lastname: Tacker
authors.forename: L A
authors.initials: LA
authors.lastname: Geddes
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001011:Aorta / Q000473:pathology / Q000601:surgery*; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D003094:Collagen; D004198:Disease Susceptibility; D004285:Dogs; D004730:Endothelium, Vascular / Q000473:pathology; D005334:Fever / Q000209:etiology; D006097:Granulation Tissue / Q000473:pathology; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology / Q000637:transplantation*; D007421:Intestine, Small / Q000601:surgery; D007964:Leukocytosis / Q000209:etiology; D011138:Polytetrafluoroethylene / Q000737:chemistry; D013203:Staphylococcal Infections; D013499:Surface Properties; D013530:Surgical Wound Infection / Q000209:etiology*; D015996:Survival Rate; D014182:Transplantation, Autologous; D014654:Vascular Patency
chemical_list: D001672:Biocompatible Materials; D011138:Polytetrafluoroethylene; D003094:Collagen