[The nuclear determination of the DNA content by flow cytometry as a fixed sample of the healthy larynx].
We analyzed the DNA nuclear content and cellular cycle distribution of the healthy laryngeal tissues without any laryngeal disease (healthy) and that of from patients afflicted with laryngeal epidermoid carcinoma (healthy/Ca). We have carried out studies on fresh samples, unfixed samples, and analysing them immediately after their extraction in the flow cytometer, using human peripheral blood lymphocytes as control of the ploidy in each case. In the study effected, we did not find any differences in the DNA content of the healthy laryngeal tissues and those macroscopically healthy from patients with epidermoid carcinoma of the larynx. However, the laryngeal tissues show a significantly higher DNA content than the human peripheral blood lymphocytes used as check.
Mellado Rubio
R
R
Servicio de ORL, Hospital Regional Universitario Reina Sofía, Córdoba.
García Castellano
J M
JM
Cantillo Baños
E
E
Povedano Rodríguez
V
V
Jurado Ramos
A
A
López Villarejo
P
P
D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000473:pathology; D046508:Culture Techniques; D004247:DNA / Q000737:chemistry*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007830:Larynx / Q000737:chemistry / Q000473:pathology / Q000502:physiology*; D008214:Lymphocytes / Q000737:chemistry; D008297:Male
D004247:DNA
Contenido de ADN nuclear por citometría de flujo en muestras no fijadas de laringe sana.