The signal transduction mechanism responsible for gamma interferon-induced indoleamine 2,3-dioxygenase gene expression.
We examined the signal transduction mechanism responsible for the gamma interferon-induced indoleamine 2,3-dioxygenase (IDO) gene expression in a human monocytic cell line, THP-1. Our results suggest that gamma interferon-induced activation of protein tyrosine kinase is a prerequisite for gene expression and that activation of protein kinase C and another unknown signal(s), both of which are transduced by the protein tyrosine kinase, synergistically induce IDO gene expression. Neither Ca2+ influx nor cyclic nucleotide-dependent kinases were suggested to be involved in the signaling pathway.
Koide
Y
Y
Department of Microbiology, Hamamatsu University School of Medicine, Japan.
Yoshida
A
A
D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology; D002460:Cell Line; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007371:Interferon-gamma / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology; D009712:Nucleotides, Cyclic / Q000502:physiology; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction; D014366:Tryptophan Oxygenase / Q000235:genetics*; D020032:Tyrphostins
D002396:Catechols; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D009570:Nitriles; D009712:Nucleotides, Cyclic; D012333:RNA, Messenger; D020032:Tyrphostins; C060152:tyrphostin 47; D007371:Interferon-gamma; D014366:Tryptophan Oxygenase; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D002118:Calcium