title: Compared kinetics of plasma creatine kinase activity in rabbits after intravenous injection of different preparations of skeletal muscle.
abstract: The purpose of this study was to compare the disposition parameters of creatine kinase (CK) in rabbits after intravenous bolus administration of 3 CK preparations: 1) purified CK; 2) CK obtained from a muscular extract after a 3,000 g centrifugation (ie with cell remains) (3,000 g CK); or 3) a 105,000 g centrifugation (ie the cytosolic soluble phase) (105,000 g CK). The plasma half-lives were not significantly different (approximately 9 h). In contrast, the clearance of 3,000 g CK (3.25 +/- 0.33 ml.kg-1.h-1) was significantly lower than that of the 2 others (7.00 +/- 0.49 ml.kg-1.h-1 and 4.63 +/- 0.65 ml.kg-1.h-1 for 105,000 g and purified CK, respectively). These findings suggest that purified CK preparation is not the most appropriate form for determination of enzyme pharmacokinetic parameters following muscle damage.
authors.affiliation: INRA, Département de Physiopathologie et URA, Toulouse, France.
authors.forename: H P
authors.initials: HP
authors.lastname: Lefebvre
authors.forename: P L
authors.initials: PL
authors.lastname: Toutain
authors.forename: L
authors.initials: L
authors.lastname: Bret
authors.forename: A G
authors.initials: AG
authors.lastname: Rico
authors.forename: J P
authors.initials: JP
authors.lastname: Braun
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003402:Creatine Kinase / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D005260:Female; D006207:Half-Life; D007275:Injections, Intravenous / Q000662:veterinary; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits
chemical_list: D003402:Creatine Kinase