Contamination of genetically engineered CHO-cells by epizootic haemorrhagic disease virus (EHDV).
The characterization of a contaminating virus which was detected in genetically-engineered Chinese hamster ovary (CHO) cells during the production of biologicals is described in the present paper. Under electron microscopy, the contaminating virus had a morphology resembling that of an orbivirus. The relationship was confirmed by nucleic acid analysis which showed a RNA segment pattern characteristic of orbiviruses. With an immunoperoxidase staining of monolayer cells and through sero-neutralization tests the virus was identified as being identical to Epizootic Haemorrhagic Disease Virus (EHDV), isolate 318 (untyped) from Bahrain. Potential sources of the contaminating virus and feasible procedures to avoid adventitous virus infections in cell cultures are discussed.
Rabenau
H
H
University Clinics of Frankfurt/Main, Institute of Medical Virology, Germany.
Ohlinger
V
V
Anderson
J
J
Selb
B
B
Cinatl
J
J
Wolf
W
W
Frost
J
J
Mellor
P
P
Doerr
H W
HW
D000818:Animals; D016466:CHO Cells / Q000382:microbiology*; D002478:Cells, Cultured; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D005818:Genetic Engineering; D018164:Hemorrhagic Disease Virus, Epizootic / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D009500:Neutralization Tests; D014407:Tumor Cells, Cultured