title: Determination of tris(hydroxymethyl)aminomethane (tromethamine) in human plasma and urine by high-performance liquid chromatography with fluorescence detection.
abstract: Determination of tromethamine (2-amino-2-hydroxymethyl-1,3-propanediol) in human plasma and urine has been developed and validated. The method utilizes reversed-phase high-performance liquid chromatographic separation and fluorescence detection of a pre-column derivative. The assay was linear in the ranges of 1-200 micrograms/ml plasma and 5-500 micrograms/ml urine with accuracies (mean percent recovery) all within 10% of 100% and precisions (coefficient of variation) all less than 10%.
authors.affiliation: Merck Research Laboratories, West Point, PA 19486.
authors.forename: M J
authors.initials: MJ
authors.lastname: Morris
authors.forename: J Y
authors.initials: JY
authors.lastname: Hsieh
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013050:Spectrometry, Fluorescence; D014325:Tromethamine / Q000032:analysis*
chemical_list: D014325:Tromethamine