title: Binding of the cytosolic p200 protein to Golgi membranes is regulated by heterotrimeric G proteins.
abstract: The formation of vesicles for protein trafficking requires the dynamic binding of cytosolic coat proteins onto Golgi membranes and this binding is regulated by a variety of GTPases, including heterotrimeric G proteins. We have previously shown the presence of the pertussis toxin-sensitive G alpha i-3 protein on Golgi membranes and demonstrated a functional role for G alpha i-3 in the trafficking of secretory proteins through the Golgi complex. We have also described a brefeldin A-sensitive phosphoprotein, p200, which is found in the cytoplasm and on Golgi membranes. The present study investigates the role of heterotrimeric G proteins in the regulation of p200 binding to Golgi membranes. An in vitro binding assay was used to measure the binding of cytosolic p200 to LLC-PK1 cell microsomal membranes and to purified rat liver Golgi membranes in the presence of specific activators of G proteins. The binding of p200 to Golgi membranes was compared to that of the coatomer protein beta-COP, for which G protein-dependent membrane binding has previously been established. Membrane binding of both p200 and beta-COP was induced maximally by activation of all G proteins in the presence of GTP gamma S. More selective activation of the heterotrimeric G proteins, with AlFn or mastoparan, also induced membrane binding of p200 and beta-COP. Pertussis toxin pretreatment of Golgi membranes, to selectively inactivate G alpha i-3, reduced the AlFn and mastoparan-induced binding of p200 to Golgi membranes, whereas no significant effect of pertussis toxin on beta-COP binding was found in this assay. The effect of pertussis toxin thus implicates G alpha i-3, as one component of a regulatory pathway, in the binding of cytosolic p200 to Golgi membranes. The effects of AlFn and pertussis toxin on p200 membrane binding were also shown in intact cells by immunofluorescence staining. AlFn treatment of cells induced translocation of p200 from the cytoplasm onto the Golgi complex, resulting in a conformational change in some Golgi membranes. The translocation of p200 was blocked by pretreatment of intact NRK cells with pertussis toxin. The data presented here support the conclusion that the binding of the p200 protein to Golgi membranes involves regulation by the pertussis toxin-sensitive heterotrimeric G proteins, specifically the G alpha i-3 protein.
authors.affiliation: Renal Unit, Massachusetts General Hospital, Charlestown.
authors.forename: J B
authors.initials: JB
authors.lastname: de Almeida
authors.forename: J
authors.initials: J
authors.lastname: Doherty
authors.forename: D A
authors.initials: DA
authors.lastname: Ausiello
authors.forename: J L
authors.initials: JL
authors.lastname: Stow
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D002451:Cell Compartmentation / Q000187:drug effects; D020756:Coatomer Protein; D003600:Cytosol / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005459:Fluorides / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D036341:Intercellular Signaling Peptides and Proteins; D007425:Intracellular Membranes / Q000378:metabolism* / Q000648:ultrastructure; D007668:Kidney / Q000166:cytology; D008099:Liver / Q000166:cytology; D008861:Microsomes / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010455:Peptides; D037342:Pertussis Toxin; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D013552:Swine; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D014862:Wasp Venoms / Q000494:pharmacology
chemical_list: D017607:Aluminum Compounds; D020756:Coatomer Protein; D036341:Intercellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D010455:Peptides; D010566:Virulence Factors, Bordetella; D014862:Wasp Venoms; D016244:Guanosine 5'-O-(3-Thiotriphosphate); C037500:mastoparan; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D005459:Fluorides; C032311:aluminum fluoride