Heterogeneity of carbonic anhydrase and 68 kDa neurofilament in nerve roots analyzed by two-dimensional electrophoresis.
The heterogeneity of carbonic anhydrase (CA) and 68 kDa neurofilament (NF) in nerve roots of Japanese monkeys (Macaca Macacus) was analyzed by two-dimensional electrophoresis combined with immunoblotting. CA was detected as heterogeneous spots of type I and II having the same molecular weight (M(r)) but different isoelectric point (pI), in the range of pH 4.5-5.5. CA had much higher concentrations in soluble fractions of dorsal roots than ventral roots but not in membrane-bound fractions of either root. NF was detected as a single spot at the position of 68 kDa and pI 5.2 in soluble fractions of both roots, however, it was detected in the membrane-bound fractions as heterogeneous spots having different molecular masses and pI in the range of 60-68 kDa and 5.0-5.5, respectively. The presence of heterogeneous CA type I and II at high concentrations in dorsal roots enables us to distinguish the sensory nerves from the motor nerves. Furthermore, the heterogeneity of NF in membrane-bound fractions gives us information for understanding the dynamic assembly and the degradation of NF in nerve roots.
Fujii
H
H
Department of Orthopedic Surgery, Yamaguchi University School of Medicine, Ube, Japan.
Nakamura
K
K
Takeo
K
K
Kawai
S
S
D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D002256:Carbonic Anhydrases / Q000032:analysis*; D005591:Chemical Fractionation; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D008251:Macaca; D008970:Molecular Weight; D009454:Neurofibrils / Q000737:chemistry*; D010525:Peripheral Nerves / Q000737:chemistry*; D012995:Solubility; D013126:Spinal Nerve Roots / Q000737:chemistry* / Q000201:enzymology
D015415:Biomarkers; D002256:Carbonic Anhydrases