Insulin-like growth factors counteract the effect of interleukin 1 beta on type II phospholipase A2 expression and arachidonic acid release by rabbit articular chondrocytes.
Interleukin 1 beta was found to stimulate arachidonic acid release, and the synthesis and secretion of type II phospholipase A2 by rabbit articular chondrocytes in vitro. Interleukin 1 beta had no effect on the level of cytosolic phospholipase A2 mRNA. Insulin-like growth factors, which help stabilize the cartilage matrix, reduced the effect of interleukin 1 beta on type II phospholipase A2 activity and mRNA level, and decreased the Interleukin 1 beta-stimulated arachidonic acid release to the basal values. This suggests that type II phospholipase A2 plays a key role in arachidonic acid release from rabbit articular chondrocytes and that insulin-like growth factors counteract the effect of interleukin 1 beta. They may therefore be considered as potential antiinflammatory agents.
Berenbaum
F
F
URA CNRS 1283, Faculté de Médecine Saint Antoine, Paris, France.
Thomas
G
G
Poiraudeau
S
S
Béréziat
G
G
Corvol
M T
MT
Masliah
J
J
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D007375:Interleukin-1 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D010741:Phospholipases A / Q000096:biosynthesis* / Q000235:genetics; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits
D007375:Interleukin-1; D012333:RNA, Messenger; D016718:Arachidonic Acid; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D010741:Phospholipases A; D054467:Phospholipases A2