title: Preliminary crystallographic studies of the ligand-binding domain of the thyroid hormone receptor complexed with triiodothyronine.
abstract: A truncated, recombinant form of the thyroid hormone receptor, including the hormone binding domain, has been co-crystallized with the hormone T3. The crystals are monoclinic, most likely space group P2, with two molecules per asymmetric unit and cell dimensions a = 63.6 A, b = 80.8 A, c = 100.9 A and beta = 92.1 degrees. The crystals diffract to only medium resolution and decay rapidly in the X-ray beam using laboratory sources. By contrast, high resolution, high-quality data are obtained using synchrotron radiation in conjunction with cryocrystallography.
authors.affiliation: Department of Biochemistry and Biophysics, University of California San Francisco 94143.
authors.forename: M E
authors.initials: ME
authors.lastname: McGrath
authors.forename: R L
authors.initials: RL
authors.lastname: Wagner
authors.forename: J W
authors.initials: JW
authors.lastname: Apriletti
authors.forename: B L
authors.initials: BL
authors.lastname: West
authors.forename: V
authors.initials: V
authors.lastname: Ramalingam
authors.forename: J D
authors.initials: JD
authors.lastname: Baxter
authors.forename: R J
authors.initials: RJ
authors.lastname: Fletterick
mesh_terms: D001665:Binding Sites; D003461:Crystallography; D011988:Receptors, Thyroid Hormone / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D014284:Triiodothyronine / Q000737:chemistry*
chemical_list: D011988:Receptors, Thyroid Hormone; D011994:Recombinant Proteins; D014284:Triiodothyronine