Influence of cytoplasmic microinjection on meiotic competence in growing pig oocytes.
It is demonstrated that the microinjection of cytoplasm from competent, fully grown oocytes can induce germinal vesicle breakdown in meiotically incompetent growing oocytes. Injection of cytoplasm from oocytes at methaphase I had a similar effect. The maturation in injected growing oocytes was arrested at the premetaphase or metaphase I stage. Induction of germinal vesicle breakdown by cytoplasm microinjection occurs in a dose-dependent manner.
Petr
J
J
Research Institute of Animal Production, Prague, Uhrínĕves, Czech Republic.
Rozinek
J
J
Fulka
J
J
Jílek
F
F
D000818:Animals; D003593:Cytoplasm / Q000502:physiology*; D005260:Female; D008540:Meiosis / Q000502:physiology*; D008677:Metaphase; D008845:Microinjections; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure*; D013552:Swine