Monensin and forskolin inhibit the transcription rate of sucrase-isomaltase but not the stability of its mRNA in Caco-2 cells.
Treatment of Caco-2 cells with forskolin (25 microM) or monensin (1 microM) has previously been shown to cause a marked decrease in the level of sucrase-isomaltase (SI) mRNA, without any effect on the expression of dipeptidylpeptidase IV (DPP-IV). In the present work, we report that there is no significant difference in the stability of SI mRNA between control and treated cells. On the other hand, we demonstrate a decrease in the transcription rate of SI mRNA which is sufficient to account for the decrease in the steady-state level of SI mRNA both in forskolin- and monensin-treated Caco-2 cells.
Chantret
I
I
Unité de Recherches sur la Différenciation Cellulaire Intestinale, INSERM U178, Villejuif, France.
Lacasa
M
M
Chevalier
G
G
Swallow
D
D
Rousset
M
M
D015152:Blotting, Northern; D005576:Colforsin / Q000494:pharmacology*; D015342:DNA Probes; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D008985:Monensin / Q000494:pharmacology*; D009834:Oligo-1,6-Glucosidase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013393:Sucrase / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D012333:RNA, Messenger; D005576:Colforsin; D008985:Monensin; D009834:Oligo-1,6-Glucosidase; D013393:Sucrase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4