title: Demonstration of glibenclamide-sensitive K+ fluxes in rat liver mitochondria.
abstract: Influx of K+ into rat liver mitochondria driven by the transmembrane potential was investigated. Under non-energized conditions (non-respiring mitochondria suspended in isotonic KSCN) K+ influx (manifested by mitochondrial swelling) was enhanced by pinacidil and its derivative P1060, known as K+ channel openers in the plasma membrane; this stimulation being inhibited by potassium channel blocker glibenclamide. Under energized conditions (respiring mitochondria) the rate of K+ uptake (measured with K(+)-sensitive electrode) was increased by P1060 and slowed down by glibenclamide. These results indicate functioning of a specific potassium channel in intact liver mitochondria.
authors.affiliation: Nencki Institute of Experimental Biology, Warsaw, Poland.
authors.forename: E A
authors.initials: EA
authors.lastname: Belyaeva
authors.forename: A
authors.initials: A
authors.lastname: Szewczyk
authors.forename: B
authors.initials: B
authors.lastname: Mikołajek
authors.forename: M J
authors.initials: MJ
authors.lastname: Nałecz
authors.forename: L
authors.initials: L
authors.lastname: Wojtczak
mesh_terms: D000818:Animals; D005905:Glyburide / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D020110:Pinacidil; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006146:Guanidines; D015221:Potassium Channels; C058754:N'-cyano-N-(3-pyridyl)-N''-(t-butyl)guanidine; D020110:Pinacidil; D011188:Potassium; D010100:Oxygen; D005905:Glyburide