Immunolocalization of scrapie amyloid in non-congophilic, non-birefringent deposits in golden Syrian hamsters with experimental transmissible mink encephalopathy.
Transmissible mink encephalopathy (TME), a naturally occurring subacute spongiform encephalopathy in commercially ranch-reared mink (Mustela vision), is characterized neuropathologically by spongiform changes in the neuropil, intracytoplasmic neuronal vacuolation and astrocytic hypertrophy and hyperplasia. Amyloid deposits have not been observed in brain tissue sections from animals with natural and experimental TME using conventional histochemical stains such as Congo red. To determine if amyloid deposits be visualized by immunocytochemical techniques, we stained formalin-fixed, formic acid-treated brain tissue sections from several animal species with natural and experimental TME, using a rabbit antiserum directed against scrapie amyloid (PrP27-30). Scrapie amyloid-immunoreactive deposits were found in golden Syrian hamsters experimentally infected with TME, but were absent in mink with natural and experimental TME, as well as in ferrets and squirrel monkeys with experimental TME. The scrapie amyloid-immunoreactive deposits, which were non-congophilic and non-birefringent, were distributed in the subependymal, subpial and perivascular regions of the brain, as in hamsters infected with the 263K strain of scrapie. Ultrastructurally, scrapie amyloid-immunoreactive deposits revealed a collection of degenerating neurites with numerous abnormal mitochondria and degenerating synapses. Amyloid fibrils were not observed. Anti-scrapie amyloid antibodies immunoabsorbed with scrapie amyloid abolished immunostaining. Our data indicate the presence of scrapie amyloid lacking the molecular conformation of amyloid fibrils in hamsters with experimental TME.
Guiroy
D C
DC
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institute of Health, Bethesda, MD 20892.
Marsh
R F
RF
Yanagihara
R
R
Gajdusek
D C
DC
D000818:Animals; D001718:Birefringence; D001921:Brain / Q000378:metabolism; D001927:Brain Diseases / Q000378:metabolism*; D003224:Congo Red; D006224:Cricetinae / Q000378:metabolism*; D005289:Ferrets; D007150:Immunohistochemistry; D008647:Mesocricetus; D008907:Mink; D018622:PrP 27-30 Protein; D017096:Prion Diseases / Q000378:metabolism*; D011328:Prions / Q000378:metabolism*; D012453:Saimiri; D014018:Tissue Distribution
D011328:Prions; D018622:PrP 27-30 Protein; D003224:Congo Red