Serum antipyrine concentrations determined by micellar electrokinetic capillary chromatography.
We developed an assay for antipyrine in serum using micellar electrokinetic capillary chromatography. A capillary electrophoresis system with a 70-cm fused-silica capillary and pH 8.2 borate-sodium dodecyl sulfate buffer was used. Standard curves ranging from 0.5 microgram/ml to 25 micrograms/ml were analyzed. The resulting electropherograms showed no interfering endogenous peaks and the assay was linear in the range of concentrations analyzed. The intra-day and inter-day relative standard deviations ranged from 0.7 to 5.2% and 1.7 to 8.1%, respectively. Analytical recovery of antipyrine ranged from 78.2 to 105.0%. The theoretical plate number for antipyrine was calculated to be 561,000 +/- 79,000 plates/m.
Brunner
L J
LJ
Department of Pharmaceutics, College of Pharmacy, University of Georgia, Athens 30602.
DiPiro
J T
JT
Feldman
S
S
D000082:Acetaminophen / Q000097:blood; D000818:Animals; D000983:Antipyrine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002845:Chromatography / Q000379:methods; D004563:Electrochemistry; D004586:Electrophoresis; D007275:Injections, Intravenous; D008297:Male; D008823:Micelles; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet
D008823:Micelles; D000082:Acetaminophen; D000983:Antipyrine