Species-specific interaction of the glutamine-rich activation domains of Sp1 with the TATA box-binding protein.
We have used protein-blotting and protein affinity chromatography to demonstrate that each of the two glutamine-rich activation domains of the human transcription factor Sp1 can bind specifically and directly to the C-terminal evolutionarily conserved domain of the human TATA box-binding protein (TBP). These activation domains of Sp1 also bind directly to Drosophila TBP but bind much less strongly to TBP from the yeast Saccharomyces cerevisiae. The abilities of the Sp1 activation domains to interact directly with the TBPs of various species correlate well with their abilities to activate transcription in extracts derived from the same species. We also show that a glutamine-rich transcriptional activating region of the Drosophila protein Antennapedia binds directly to TBP in a species-specific manner that reflects its ability to activate transcription in vivo. These results support the notion that TBP is a direct and important target of glutamine-rich transcriptional activators.
Emili
A
A
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.
Greenblatt
J
J
Ingles
C J
CJ
D000818:Animals; D051943:Antennapedia Homeodomain Protein; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D005656:Fungal Proteins / Q000378:metabolism; D005973:Glutamine / Q000737:chemistry; D006367:HeLa Cells; D018398:Homeodomain Proteins; D006801:Humans; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D011485:Protein Binding; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D013045:Species Specificity; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation
D051943:Antennapedia Homeodomain Protein; C497974:Antp protein, Drosophila; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; D018398:Homeodomain Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D016329:Sp1 Transcription Factor; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; D005973:Glutamine