Glycoconjugates as components of receptors for Bordetella avium on the tracheal mucosa of turkeys.
Bordetella avium is an important respiratory tract pathogen of turkeys. In common with other pathogenic bordetellae, B avium manifests a tissue tropism for cilia of the respiratory tract epithelium. To determine the molecular characteristics of the host cell receptors for B avium, we used hemagglutination and in vivo adherence assays. Carbohydrates, mucus, sialic acid-specific lectin, and other glycoconjugates were evaluated for their ability to competitively inhibit binding of B avium to host cells. The gangliosides, GD1a and GT1b, completely inhibited hemagglutination, whereas N-acetylneuraminic acid (sialic acid) partially inhibited hemagglutination. Adherence to turkey tracheal mucosa in vivo was significantly (P < 0.01) inhibited by GD1a and GT1b gangliosides, N-acetylneuraminic acid, bovine submaxillary mucin, and horseshoe crab (Limulus polyphemus) lectin. Treatment of the tracheal mucosa with neuraminidase also inhibited adherence of B avium. We conclude that N-acetylneuraminic acid and the gangliosides, GD1a and GT1b, may be important components of the tracheal mucosa receptor for B avium in turkeys.
Arp
L H
LH
Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames 50011.
Huffman
E L
EL
Hellwig
D H
DH
D000818:Animals; D060829:Arthropod Proteins; D001422:Bacterial Adhesion / Q000187:drug effects; D001884:Bordetella / Q000502:physiology*; D002241:Carbohydrates / Q000494:pharmacology; D002417:Cattle; D005732:Gangliosides / Q000494:pharmacology; D006001:Glycoconjugates / Q000378:metabolism* / Q000494:pharmacology; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D006737:Horseshoe Crabs; D037102:Lectins / Q000494:pharmacology; D009077:Mucins / Q000494:pharmacology; D009092:Mucous Membrane / Q000382:microbiology; D014132:Trachea / Q000382:microbiology*; D014422:Turkeys / Q000382:microbiology*
D060829:Arthropod Proteins; D002241:Carbohydrates; D005732:Gangliosides; D006001:Glycoconjugates; D037102:Lectins; D009077:Mucins