title: Alcohol in the amniotic fluid prior to cesarean delivery: effects of subsequent exposure to the drug's odor upon alcohol responsiveness.
abstract: Rat fetuses during the last day of gestation have the capacity to process ethanol and non-ethanol-related chemosensory cues present in the amniotic fluid. Recent studies suggest that the consequences related to cesarean delivery act as an unconditioned stimulus that is associated with these cues. In the first experiment, ethanol neonatal responsiveness assessed through a motor activity test was analyzed in pups that received ethanol or saline in utero proximal to cesarean delivery. Different factors and the interaction among them, were analyzed in this experiment: (i) ethanol concentration administered into the amniotic sac (0, 6, or 18% v/v), (ii) delay between administration and cesarean section (3, 10, or 30 min), and (iii) postnatal exposure to ethanol odor prior to test (0, 7.5, or 15 min). Only animals exposed to ethanol 10 min prior to delivery differed from vehicle-exposed subjects. Subsequent postnatal exposure to ethanol odor attenuated the magnitude of prenatally established effects. In the second experiment it was observed that prenatal ethanol exposure was sufficient to increase ethanol intake during Postnatal Day 11. Again, this effect was strongly attenuated when pups were exposed to the odor of the drug prior to assessment procedures. These results suggest that (i) associations between chemosensory cues in the amniotic fluid and consequences related with perinatal manipulations are likely to occur and (ii) postnatal reexposure to similar cues exerts an effect comparable to an extinction phenomenon.
authors.affiliation: Instituto de Investigación Médica, Mercedes y Martín Ferreyra, Córdoba, Argentina.
authors.forename: H D
authors.initials: HD
authors.lastname: Domínguez
authors.forename: M G
authors.initials: MG
authors.lastname: Chotro
authors.forename: J C
authors.initials: JC
authors.lastname: Molina
mesh_terms: D000428:Alcohol Drinking / Q000503:physiopathology*; D000653:Amniotic Fluid / Q000378:metabolism; D000818:Animals; D001143:Arousal / Q000187:drug effects* / Q000502:physiology; D001245:Association Learning / Q000187:drug effects / Q000502:physiology; D002585:Cesarean Section; D002628:Chemoreceptor Cells / Q000187:drug effects / Q000503:physiopathology; D003214:Conditioning, Classical / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000493:pharmacokinetics / Q000494:pharmacology*; D005108:Extinction, Psychological / Q000187:drug effects / Q000502:physiology; D005260:Female; D008297:Male; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D012153:Retention, Psychology / Q000187:drug effects / Q000502:physiology; D012903:Smell / Q000187:drug effects* / Q000502:physiology
chemical_list: D000431:Ethanol