Hair cell regeneration in the European starling (Sturnus vulgaris): recovery of pure-tone detection thresholds.
Behavioral detection thresholds were obtained from four starlings before, during, and after 11 days of subcutaneous injections of kanamycin. Birds were operantly conditioned to respond to pure-tones ranging in frequency from 0.25 kHz to 7 kHz using the method of constant stimuli and were tested daily for 141 days after the first injection of aminoglycoside. All four birds sustained hearing losses greater than 60 dB at frequencies from 4 kHz to 7 kHz by the end of the 11 day injection schedule. Two birds had a slight shift in threshold at 3 kHz. No change in threshold occurred for any of the birds at lower frequencies. Recovery of detection thresholds began soon after the injections ceased and continued for approximately 50 days. In all four birds there was some degree of permanent hearing loss: 5 dB to 15 dB at frequencies between 4 kHz and 6 kHz, and approximately 25 dB at 7 kHz. Scanning electron microscopy (SEM) was performed at 0 and 5 days post-injection in a separate group of starlings given the same injection schedule. Hair cell loss and damage was observed across the basal 34% to 36% of the basilar papilla. SEM in two behaviorally tested birds sacrificed 142 days after the first injection showed that there was regeneration of hair cells to populate the previously damaged region, but that disorientation of stereocilia bundles in the basal third of the basilar papilla was common. The other two behaviorally tested birds were treated with kanamycin again for 16 days beginning at 142 days after the first injection. Thresholds shifted again, but less than during the first dosing period. SEM of these birds' basilar papillae showed less hair cell loss than observed in the birds given only a single, 11 day dosing of kanamycin. This result suggests that birds may be less susceptible to the ototoxic effects of kanamycin in repeated treatments. In all four birds, the degree and position of damage observed with SEM corresponded with the extent and frequency of hearing loss.
Marean
G C
GC
Department of Speech and Hearing Sciences, University of Washington, Seattle 98195.
Burt
J M
JM
Beecher
M D
MD
Rubel
E W
EW
D000161:Acoustic Stimulation; D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects / Q000502:physiology*; D001489:Basilar Membrane / Q000187:drug effects / Q000648:ultrastructure*; D001717:Birds; D004305:Dose-Response Relationship, Drug; D006198:Hair Cells, Auditory / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D007279:Injections, Subcutaneous; D007612:Kanamycin / Q000008:administration & dosage / Q000633:toxicity*; D008855:Microscopy, Electron, Scanning; D012038:Regeneration
D007612:Kanamycin