A rapid and sensitive hemolysis neutralization assay for palytoxin.
The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37 degrees C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of Palythoa tuberculosa.
Bignami
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Hawaii Biotechnology Group, Inc., Aiea 96701.
D000178:Acrylamides; D000818:Animals; D000911:Antibodies, Monoclonal; D003064:Cnidarian Venoms / Q000032:analysis*; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006461:Hemolysis; D007124:Immunoenzyme Techniques; D051379:Mice; D009500:Neutralization Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D000095702:Polyether Toxins
D000178:Acrylamides; D000911:Antibodies, Monoclonal; D003064:Cnidarian Venoms; C010272:palytoxin; D000095702:Polyether Toxins