The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway.
The Saccharomyces cerevisiae ERG24 gene, encoding sterol delta 14 reductase (Erg24p), was cloned by selecting strains carrying sequences on a 2 mu-based vector for resistance to the morpholine fungicide, fenpropimorph (Fp). Four distinct plasmid inserts which conferred Fp resistance (FpR) were recovered (plasmids pML99, pML100, pML101 and pM103). Although Fp is reported to inhibit activity of Erg24p and sterol delta 8-delta 7 isomerase (Erg2p; encoded by ERG2), none of the inserts had restriction maps resembling ERG2. In addition, a 2 mu plasmid overexpression of the ERG2 sequence did not produce FpR. Characterization studies were focused on plasmid pML100, because it was the only plasmid to confer FpR consistently when tested in a number of different genetic backgrounds. Tests with a panel of fungicides indicated that pML100 conferred significant resistance only to compounds (Fp, tridemorph, fenpropidin and azasterol) which have a shared site of action, Erg24p. An insertional disruption of pML100 resulted in an obligate anaerobic phenotype, indicating a lesion in sterol biosynthesis. Sterol analysis of the disrupted mutant demonstrated the accumulation of ignosterol, indicating a loss of Erg24p activity. A SphI-XbaI fragment of pML100 was sequenced, revealing the presence of an ORF encoding a 438-amino-acid protein, which is highly similar to those encoded by two previously reported yeast drug sensitivity genes, sts1+ (Schizosaccharomyces pombe) and YGL022 (S. cerevisiae). Analyses of these genes demonstrated that strains carrying disruptions of sts1+ or YGL022 have ergosterol biosynthesis defects in the enzyme, sterol C-24(28) reductase (Erg4p; encoded by ERG4).(ABSTRACT TRUNCATED AT 250 WORDS)
Lai
M H
MH
Agricultural Research Division, American Cyanamid, Princeton, NJ 08543-0400.
Bard
M
M
Pierson
C A
CA
Alexander
J F
JF
Goebl
M
M
Carter
G T
GT
Kirsch
D R
DR
D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal; D004351:Drug Resistance / Q000235:genetics; D004875:Ergosterol / Q000096:biosynthesis*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009025:Morpholines / Q000494:pharmacology; D005810:Multigene Family; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013251:Steroid Isomerases / Q000235:genetics / Q000378:metabolism
D004271:DNA, Fungal; D009025:Morpholines; C037574:morpholine; D010088:Oxidoreductases; C022673:delta 24-sterol reductase; C022672:delta(14)-sterol reductase; D013251:Steroid Isomerases; C022669:delta(8)-delta(7)-sterol isomerase; D004875:Ergosterol