A simple assay for fluorescent siderophores produced by Pseudomonas species and an efficient isolation of pseudobactin.
Several iron binding metabolites (siderophores) of Pseudomonas fluorescens B10 (JL-3133) have been detected using C18 reverse phase HPLC coupled with photodiode array detection methods. This analysis utilized a volatile mobile phase of 90% 20 mM NH4HCO3/10% MeOH, pH 6.5. It has been shown to be applicable to other P. fluorescens strains for the detection of related metabolites. Direct scale-up of the analytical HPLC conditions allowed for the efficient preparative isolation of pseudobactin, the principle siderophore produced by P.fluorescens B10 (JL-3133).
Nowak-Thompson
B
B
Department of Chemistry, Oregon State University, Corvallis 97331-4003.
Gould
S J
SJ
D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D009842:Oligopeptides / Q000096:biosynthesis / Q000302:isolation & purification*; D010860:Pigments, Biological / Q000032:analysis* / Q000302:isolation & purification; D011551:Pseudomonas fluorescens / Q000737:chemistry*; D017262:Siderophores / Q000032:analysis*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet
D009842:Oligopeptides; D010860:Pigments, Biological; D017262:Siderophores; C033038:pseudobactin