The cochlear blood flow: a comparison between the laser Doppler and the microsphere surface methods.
Since the introduction in the early 1980s, of the laser Doppler (LD) method for measuring cochlear blood flow (CBF) it has been debated whether the measured changes reflect the total or regional blood flow and whether the method per se influences the CBF. In order to answer those questions, the effect of one vasodilating drug, sodium nitroprusside, was investigated after topical application on the round window membrane (RWM) with respect to its influence on CBF. Two different techniques, the microspheres surface method and the LD method, were used. Untreated animals and animals which received saline or nicotine acid on the RWM were used as controls. The effects on CBF and blood pressure (BP) were continuously registered with LD. When a maximal flow had stabilized, 6 x 10(6) microspheres were injected into the left side of the heart. After the microspheres had been distributed within the body, the animals were killed. Both cochleae were microdissected and the microspheres counted turn by turn in the lateral wall. The number of spheres in the two ears was compared and the difference was recorded as the increase caused by the drug. The percentage change in CBF measured using the LD was compared with that obtained by using the microsphere surface method (MSM). No change in CBF measured by the two techniques was registered in the untreated animals, or after saline or nicotinic acid, while sodium nitroprusside induced a substantial increase in CBF. The mean percentage change of CBF measured with the LD method was compared with the calculated mean percentage change of microspheres for all turns in the cochlea, and in the first turn. Student's t-test and the linear correlation coefficient were calculated.(ABSTRACT TRUNCATED AT 250 WORDS)
Ohlsén
A
A
Department of Otorhinolaryngology, University Hospital, Uppsala, Sweden.
Hultcrantz
E
E
Larsen
H C
HC
Angelborg
C
C
D000287:Administration, Topical; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D002244:Carbon; D003051:Cochlea / Q000098:blood supply*; D005260:Female; D006168:Guinea Pigs; D017078:Laser-Doppler Flowmetry; D008297:Male; D008863:Microspheres; D009525:Niacin / Q000008:administration & dosage / Q000494:pharmacology; D009599:Nitroprusside / Q000008:administration & dosage / Q000494:pharmacology; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology; D012405:Round Window, Ear / Q000187:drug effects; D012965:Sodium Chloride
D009599:Nitroprusside; D009525:Niacin; D012965:Sodium Chloride; D002244:Carbon