Molecular species occurring in naturally induced interferon alpha.
Human interferon alpha (HuIFN-alpha) preparations induced in human peripheral white blood cells by Sendai virus were analyzed for activity after ultrafiltration, ion exchange, metal ion chromatography and isoelectrofocusing. It was found that HuIFN-alpha forms complexes with various physical properties and activities. Some of the IFN-alpha copurify with high molecular weight complexes. However, the low molecular weight fraction represent bulk of IFN activity. In isoelectrofocusing experiments, the former corresponds to IFN-alpha which possesses high pI (pH regions 8.5-10.5). An additional form of IFN-alpha are not complexed and possess a pI of 5.5-7.0. Our nIFN-alpha preparation preserve many of natural IFN isospecies and may have higher therapeutic value than the recombinant IFN-alpha preparations.
Kruzel
M L
ML
Amarillo Cell Culture Co. Inc. 79109.
Seman
G
G
Georgiades
J A
JA
D002845:Chromatography; D006801:Humans; D016898:Interferon-alpha / Q000032:analysis* / Q000302:isolation & purification; D007526:Isoelectric Point; D008970:Molecular Weight
D016898:Interferon-alpha