The polymorphic epithelial mucin MUC1 in human endometrium is regulated with maximal expression in the implantation phase.
After ovulation, progesterone stimulates a temporally regulated secretory transformation in human endometrial epithelium. Using a combination of immunohistochemistry, and Western and Northern blotting, we demonstrate that 1) the polymorphic epithelial mucin MUC1 is secreted by human endometrial epithelium; 2) low levels of both mRNA and core protein are present in the preovulatory phase of the menstrual cycle; 3) mRNA levels increase several-fold after ovulation, consistent with transcriptional regulation by progesterone; 4) there is an increase in translation product in postovulatory endometrium; and 5) the tandem repeat domain of the MUC-1 polypeptide is glycosylated in endometrium.
Hey
N A
NA
Department of Obstetrics and Gynaecology, University of Manchester, United Kingdom.
Graham
R A
RA
Seif
M W
MW
Aplin
J D
JD
D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D015152:Blotting, Northern; D015153:Blotting, Western; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D010064:Embryo Implantation; D004717:Endometrium / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D006031:Glycosylation; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D008597:Menstrual Cycle / Q000502:physiology; D018396:Mucin-1; D009077:Mucins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D010060:Ovulation / Q000502:physiology; D016612:Paraffin Embedding; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D018396:Mucin-1; D009077:Mucins; D012333:RNA, Messenger; D011374:Progesterone; D004247:DNA; D009439:Neuraminidase