Characterization of the lipopolysaccharide of Moraxella catarrhalis. Structural analysis of the lipid A from M. catarrhalis serotype A lipopolysaccharide.
The lipopolysaccharide of Moraxella catarrhalis serotype A (ATCC 25238) was found to consist of a short-chain oligosaccharide attached to a lipid A moiety. Composition and NMR analyses showed the oligosaccharide component in O-deacylated LPS to be composed of D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose and 3-deoxy-D-manno-octulosonic acid in the molar ratio of 5:2:1:2. In addition, the lipid A region contained phosphate, D-glucosamine, 3-hydroxydodecanoic acid, dodecanoic acid and decanoic acid. The lipid A was examined in detail by high-field NMR spectroscopy and mass spectrometry. It was found to consist of a beta-1,6-D-glucosamine disaccharide backbone esterified at C4' by a phosphomonoester and glycosidically at C1 by diphosphoethanolamine or phosphomonoester. The amide group of the reducing and nonreducing glucosamine residues were acylated by 3-dodecanoyloxydodecanoic acid and 3-decanoyl-oxydodecanoic acid, respectively. The hydroxyl group at C3 and C3' were acylated by 3-decanoyl-oxydodecanoic acid and 3-hydroxydodecanoic acid respectively, while the hydroxyl groups at C4 and C6' were unsubstituted.
Masoud
H
H
Institute for Biological Sciences, National Research Council of Canada, Ottawa.
Perry
M B
MB
Richards
J C
JC
D002240:Carbohydrate Sequence; D005227:Fatty Acids / Q000032:analysis; D006900:Hydroxylation; D008050:Lipid A / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D001936:Moraxella catarrhalis / Q000737:chemistry* / Q000145:classification; D010710:Phosphates / Q000737:chemistry; D012703:Serotyping; D016339:Spectrometry, Mass, Fast Atom Bombardment
D005227:Fatty Acids; D008050:Lipid A; D010710:Phosphates