Monitoring of benzodiazepines (clobazam, diazepam and their main active metabolites) in human plasma by column-switching high-performance liquid chromatography.
A column-switching high-performance liquid chromatographic method for the simultaneous determination of clobazam, diazepam and their main metabolites in human plasma is described. A 200-microliters plasma sample was directly injected into a precolumn filled with TSK-gel PW. After a washing step with potassium phosphate buffer, the retained substances were backflushed into a reversed-phase column with a mobile phase of acetonitrile-phosphate buffer-diethylamine. Various drugs frequently co-administered with clobazam or diazepam do not interfere with the determination.
Lacroix
C
C
Laboratoire de Pharmacocinétique, Centre Hospitalier, Le Havre, France.
Wojciechowski
F
F
Danger
P
P
D014151:Anti-Anxiety Agents / Q000097:blood* / Q000378:metabolism; D001569:Benzodiazepines; D001570:Benzodiazepinones / Q000097:blood*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000078306:Clobazam; D003975:Diazepam / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D014151:Anti-Anxiety Agents; D001570:Benzodiazepinones; D001569:Benzodiazepines; D000078306:Clobazam; D003975:Diazepam