Ultrathin-layer zone electrophoresis of lactate dehydrogenase isoenzymes in microdissected liver samples.
A highly sensitive electrophoretic separation of lactate dehydrogenase isoenzymes in 150-microns ultrathin-layer polyacrylamide gels is described. By means of the incorporation of miniature-sized wells into the gel and by executing all the analytical steps under paraffin oil, this technique allows the exact and sharp separation of the lactate dehydrogenase isoenzymes in nanogram-sized microdissected liver tissue samples. With this method the heterogeneous distribution pattern of lactate dehydrogenase isoenzymes in the liver acinus of various mammals could be demonstrated, and a new interpretation of their functional roles is offered.
Maly
I P
IP
Anatomisches Institut der Universität Basel, Switzerland.
Toranelli
M
M
D000818:Animals; D004210:Dissection; D004586:Electrophoresis / Q000379:methods; D005260:Female; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000502:physiology; D008099:Liver / Q000201:enzymology*; D008297:Male; D008846:Micromanipulation; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012418:Ruminants; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013552:Swine
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase