title: Pharmacokinetics, biodistribution and tumor localization of two anti-human B-cell chronic lymphocytic leukemia monoclonal antibodies and their F(ab)'2 fragments in a xenograft model.
abstract: We investigated the pharmacokinetics, biodistribution and tumor localization of intravenously injected Dal B01 and Dal B02, two monoclonal antibodies (MoAbs) directed against tumor associated antigens on human chronic lymphocytic leukemia (CLL) B cells, and their F(ab)'2 fragments in nude mice bearing xenografts of the human B cell CLL line D10-1. More of the percentages of the injected dose (% ID) of these two MoAbs and their F(ab)'2 fragments specifically localized in the tumor xenografts than in normal tissues. Compared to intact MoAbs, their F(ab)'2 fragments had lower % ID in tumors and were cleared from circulation faster. Well-defined tumor images were obtained at 24 and 48 h after administration of [131I]Dal B02 F(ab)'2 fragment and at 96-192 h after administration of [131I]Dal B02. A comparison between intravenous and intraperitoneal routes of administration of [131I]Dal B02 did not reveal any difference in the localization of % ID in tumor or normal tissues.
authors.affiliation: Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhu
authors.forename: T
authors.initials: T
authors.lastname: Ghose
authors.forename: S
authors.initials: S
authors.lastname: Iles
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: L A
authors.initials: LA
authors.lastname: Fernandez
authors.forename: C L
authors.initials: CL
authors.lastname: Lee
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D001345:Autoradiography; D004195:Disease Models, Animal; D005260:Female; D015902:Gamma Cameras; D006801:Humans; D007128:Immunoglobulin Fragments / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D000922:Immunotoxins / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007457:Iodine Radioisotopes; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000000981:diagnostic imaging / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
chemical_list: D000911:Antibodies, Monoclonal; D007128:Immunoglobulin Fragments; D000922:Immunotoxins; D007457:Iodine Radioisotopes