Improvement of culture conditions for L-proline production by a recombinant strain of Serratia marcescens.
Serratia marcescens SP511 was previously reported to be an L-proline-producing strain that harbors a recombinant plasmid carrying the mutant type of the proline operon. This strain produced 65 g/L of L-proline in a medium containing 22% sucrose and urea after 5 d of incubation under the conventional culture conditions. We searched for more suitable culture conditions for more abundant L-proline production by SP511. To improve the supply of a nitrogen source to cells, ammonium was used instead of urea and fed to a culture under control of the pH of the medium. The concentrations of MgSO4 and K2HPO4 were increased, and in addition, sucrose was continuously added to the culture at a final concentration of 32%. Under these conditions, the cell amount was increased twofold over that under the previous conditions and L-proline production reached a maximum of more than 100 g/L after 4 d of incubation.
Masuda
M
M
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Takamatu
S
S
Nishimura
N
N
Komatsubara
S
S
Tosa
T
T
D000641:Ammonia / Q000378:metabolism; D003470:Culture Media; D004274:DNA, Recombinant; D006863:Hydrogen-Ion Concentration; D008278:Magnesium Sulfate / Q000378:metabolism; D010710:Phosphates; D010957:Plasmids; D017680:Potassium Compounds; D011392:Proline / Q000096:biosynthesis*; D012706:Serratia marcescens / Q000235:genetics / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013395:Sucrose / Q000378:metabolism; D014508:Urea / Q000378:metabolism
D003470:Culture Media; D004274:DNA, Recombinant; D010710:Phosphates; D017680:Potassium Compounds; D013395:Sucrose; D008278:Magnesium Sulfate; D000641:Ammonia; D014508:Urea; D011392:Proline; C013216:potassium phosphate