A gene cassette for adapting Escherichia coli strains as hosts for att-Int-mediated rearrangement and pL expression vectors.
A cassette of genes from bacteriophage lambda, when carried on a derivative of bacteriophage Mu, renders strains of Escherichia coli (and in principle other Mu-sensitive bacteria) capable of supporting lambda-based expression vectors, such as rearrangement vectors and pL vectors. The gene cassette contains a temperature-sensitive allele of the repressor gene, cIts857, and a shortened leftward operon comprising, oLpL, N, xis and int. Transfection and lysogenization of this cassette into various host bacteria is mediated by phage Mu functions. Examples of regulated expression of the gene encoding T4 DNA ligase are presented.
Balakrishnan
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OmniGene Inc., Cambridge, MA 02139-9002.
Bolten
B
B
Backman
K C
KC
D010582:Bacteriophage lambda / Q000235:genetics; D010583:Bacteriophage mu / Q000235:genetics; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D016254:Mutagenesis, Insertional; D015183:Restriction Mapping