Studies on a glucose-binding lectin from peripheral blood lymphocytes.
Lectin-carbohydrate interactions have been found to be important in many of the steps of lymphocyte recirculation and inflammatory responses. A D-glucose-specific lectin was isolated from goat peripheral blood lymphocytes by affinity chromatography on N-acetyl-D-glucosamine agarose and gave a single band corresponding to 112 kDa in SDS-PAGE, irrespective of treatment with 2-mercaptoethanol. The lymphocyte lectin agglutinated rabbit and human ABO erythrocytes, the hemagglutinating activity being Ca2+ dependent. It appears to be a member of type C animal lectins.
Kayestha
R
R
School of Life Sciences, Devi Ahilya Vishwavidyalay, Vigyan Bhawan, Indore, India.
Berry
A
A
Hajela
K
K
D000818:Animals; D002118:Calcium / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D006041:Goats; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000097:blood* / Q000302:isolation & purification; D008214:Lymphocytes / Q000378:metabolism*
D037102:Lectins; D005947:Glucose; D002118:Calcium