Monoclonal non-specific suppressor factor (MNSF) inhibits the IL4 secretion by bone marrow-derived mast cell (BMMC).
We investigated whether monoclonal non-specific suppressor factor (MNSF) has an effect on the IL4 secretion by BMMC sensitized with anti-DNP IgE monoclonal antibody. The sensitized BMMC showed a maximum increase of IL4 secretion at 10 ng/ml antigen activation. When MNSF (10 U) was added, it remarkably suppressed the IL4 secretion (82 +/- 3%). MNSF also suppressed the IL4 secretion by the mast cell line, CFTL-15, but not CFTL-12. Northern blot analysis showed that, when the same amounts of MNSF were added, IL4 mRNA expression was decreased, suggesting that the suppression results from the inhibition of a transcript.
Nakamura
M
M
Department of Biochemistry, Shimane Medical University, Izumo, Japan.
Xavier
R M
RM
Tanigawa
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015152:Blotting, Northern; D001854:Bone Marrow Cells; D004140:Dinitrophenols / Q000276:immunology; D005260:Female; D007073:Immunoglobulin E / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013491:Suppressor Factors, Immunologic / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D004140:Dinitrophenols; D012333:RNA, Messenger; D013491:Suppressor Factors, Immunologic; C072169:monoclonal nonspecific suppressor factor; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma