Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus.
Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.
Jacinto
A
A
Laboratório de Genética Molecular, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
Neves
A M
AM
Vassilevskaia
T D
TD
Ricardo
C P
CP
Rodrigues-Pousada
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D004247:DNA / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D014674:Plant Proteins, Dietary / Q000235:genetics*; D014452:Ubiquitins / Q000235:genetics*
D003062:Codon; D014674:Plant Proteins, Dietary; D014452:Ubiquitins; D004247:DNA