On the cytotoxicity of irradiated media. To what extent are stable products of radial chain reactions in physiological saline responsible for cell death?
In a previous publication (Czapski et al. 1992) we reported that HOCl accounts for the toxicity of irradiated phosphate-buffered saline towards Escherichia coli bacterial cells. We have now investigated the respective toxicities towards lambda phage and mammalian cells. For phage, as with bacteria, cytotoxicity of the irradiated media seems to derive from HOCl without detectable contribution of H2O2. Mammalian cells (V79 CHO), in contrast, are more sensitive to H2O2 than to HOCl. Both agents, however, are not able to account quantitatively for the toxicity of irradiated solutions towards V79 cells; a hitherto unidentified chlorine/oxygen derivative--being formed in the sub-micromolar concentration range--is suggested to be responsible for toxicity in the case of eukaryotes.
Saran
M
M
Institut für Strahlenbiologie, GSF Research Center Neuherberg, Oberschleissheim.
Bertram
H
H
Bors
W
W
Czapski
G
G
D000818:Animals; D010582:Bacteriophage lambda / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D006861:Hydrogen Peroxide / Q000633:toxicity*; D006997:Hypochlorous Acid / Q000633:toxicity*; D010710:Phosphates / Q000528:radiation effects*; D012965:Sodium Chloride / Q000528:radiation effects*
D010710:Phosphates; D012965:Sodium Chloride; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide