Early alterations of actin in cultured human keratinocytes and fibroblasts exposed to long-wavelength radiations. Possible involvement in the UVA-induced perturbations of endocytotic processes.
Exposure of cultured MRC5 human fibroblasts or NCTC 2544 human keratinocytes to mild doses of ultraviolet A (UVA: 320-400 nm) radiations markedly decreased the actin reactivity with fluorescein-labeled phalloidin. This indicates a change in the degree of polymerization of actin and thus in the organization of actin filaments. Such a phenomenon might be involved in the previously reported UVA-induced inhibition of specific and nonspecific endocytotic processes.
Djavaheri-Mergny
M
M
Laboratoire de Physio-Chimie de l'Adaptation Biologique, INSERM U312, Muséum National d'Histoire Naturelle de Paris.
Pieraggi
M T
MT
Mazière
C
C
Santus
R
R
Lageron
A
A
Salvayre
R
R
Dubertret
L
L
Mazière
J C
JC
D000199:Actins / Q000528:radiation effects*; D002460:Cell Line; D004705:Endocytosis / Q000528:radiation effects*; D004815:Epidermal Growth Factor / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects*; D008077:Lipoproteins, LDL / Q000378:metabolism; D010590:Phalloidine; D014466:Ultraviolet Rays
D000199:Actins; D008077:Lipoproteins, LDL; D010590:Phalloidine; D004815:Epidermal Growth Factor