The Streptococcus pyogenes hyaluronan synthase: sequence comparison and conservation among various group A strains.
We recently cloned the hyaluronan synthase gene (hasA) from Streptococcus pyogenes (DeAngelis et al., J. Biol. Chem. 268, 19181, 1993). Since this is the first glycosaminoglycan synthase gene to be cloned and these enzymes have also not been purified, nothing is yet known at the molecular level about the similarity or relatedness of hyaluronan synthase to other gene products. We found several proteins in the sequence database with substantial similarities to hyaluronan synthase (HasA), including NodC from Rhizobium, DG42 from Xenopus, and the three chitin synthases (Chs) from Saccharomyces. Like HasA, NodC and the Chs proteins all have at least an N-acetylglucosaminyl transferase activity in common and are membrane-associated. The polymerase chain reaction was also used to show that HasA, and probably the two gene operon for hyaluronan biosynthesis (hasA/hasB), is highly conserved among Group A streptococcal strains. In nine strains, isolated from 1917 through 1993, only five silent mutations were detected. The results show that hyaluronan synthase is highly conserved within Group A strains, although another virulence factor, the M protein, varies considerably in these same strains.
DeAngelis
P L
PL
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555-0647.
Yang
N
N
Weigel
P H
PH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002687:Chitin Synthase / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D005798:Genes, Bacterial; D014453:Glucuronosyltransferase / Q000235:genetics*; D016695:Glycosyltransferases; D000076002:Hyaluronan Synthases; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012995:Solubility; D013297:Streptococcus pyogenes / Q000201:enzymology*; D014166:Transferases; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000235:genetics
D017931:DNA Primers; D008565:Membrane Proteins; D029867:Xenopus Proteins; D014166:Transferases; D016695:Glycosyltransferases; D002687:Chitin Synthase; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases