Expression of the collagen-like putative oncoprotein of Herpesvirus saimiri in transformed T cells.
Herpesvirus saimiri induces acute lymphomas and leukemias in New World primates and rabbits. Previous work revealed that a highly oncogenic group C strain 484-77 encodes and expresses a bicistronic mRNA in tumor-derived T cells and the first open reading frame (orf1) is highly homologous to collagen. With the aid of an antibody against a synthetic orf1 peptide, we now report that the orf1 collagen-like protein is expressed in rabbit tumor derived cell lines and in vitro transformed human and monkey T cells. The orf1 protein is expressed in vivo, as indicated by specific antibodies detected in the serum from a tumor-bearing rabbit.
Medveczky
M M
MM
Department of Medical Microbiology and Immunology, University of South Florida, Tampa 33612-4799.
Geck
P
P
Vassallo
R
R
Medveczky
P G
PG
D000595:Amino Acid Sequence; D000818:Animals; D002461:Cell Line, Transformed; D003094:Collagen / Q000096:biosynthesis*; D046508:Culture Techniques; D005455:Fluorescent Antibody Technique; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000096:biosynthesis*; D016366:Open Reading Frames; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011817:Rabbits; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis*
D015513:Oncogene Proteins; D014764:Viral Proteins; D003094:Collagen