Use of solid-phase PCR for enhanced detection of airborne microorganisms.
The solid-phase PCR (SP-PCR) was compared with a culture-based technique for the detection of aerosolized Escherichia coli DH1. Results with SP-PCR showed an increase in detection sensitivity over that of culture methods. Therefore, SP-PCR may be useful for the detection of airborne microorganisms which may be nonculturable because of aerosolization or sampling stress.
Alvarez
A J
AJ
Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras 00931.
Buttner
M P
MP
Toranzos
G A
GA
Dvorsky
E A
EA
Toro
A
A
Heikes
T B
TB
Mertikas-Pifer
L E
LE
Stetzenbach
L D
LD
D000391:Air Microbiology; D001483:Base Sequence; D015169:Colony Count, Microbial; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial