Inhibition of membrane fusion by lysophosphatidylcholine.
The ability of lysophosphatidylcholine to inhibit membrane fusion at subsolubilizing concentrations (between 1 and 9 mol % with respect to the membrane lipids) was examined. Fusion between N-methyldioleoylphosphatidylethanolamine (DOPE) large unilamellar vesicles (LUV) and fusion between Sendai virus and N-methyl-DOPE LUV were measured. A contents mixing fusion assay was used for LUV fusion (ANTS/DPX), and a lipid mixing assay (octadecylrhodamine B) was used for the virus fusion experiments. Lysophosphatidylcholine was effective at inhibiting both LUV fusion and Sendai virus/LUV fusion. Lysophosphatidylcholine also inhibited leakage from N-methyl-DOPE LUV, 31P nuclear magnetic resonance data were obtained of N-methyl-DOPE in the presence of lysophosphatidylcholine. Lysophosphatidylcholine stabilized the lamellar phase and reduced the incidence of nonlamellar structures at all temperatures. The destabilization of nonlamellar structures with a negative radius of curvature may be a mechanism for inhibition of fusion by lysophosphatidylcholine in these systems.
Yeagle
P L
PL
Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo 14214.
Smith
F T
FT
Young
J E
JE
Flanagan
T D
TD
D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008081:Liposomes; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008561:Membrane Fusion / Q000187:drug effects*; D010222:Parainfluenza Virus 1, Human / Q000502:physiology; D010714:Phosphatidylethanolamines; D012995:Solubility
D008081:Liposomes; D008244:Lysophosphatidylcholines; D010714:Phosphatidylethanolamines; C065399:N-methyl-1,2-dioleoylphosphatidylethanolamine