title: Prolonged treatment with 8-hydroxy-2-(di-n-propylamino)tetralin (8-OHDPAT) differently affects the serotonergic modulation of cortical acetylcholine release in male and female guinea pigs.
abstract: Acetylcholine (ACh) release from the cerebral cortex was studied in freely moving guinea pigs, implanted with epidural cups. A single dose of either 8-hydroxy-2-(di-n-propylamino)tetralin (8-OHDPAT, 0.1 mg/kg s.c.) or 5-hydroxytryptamine (5-HT, 250 micrograms i.c.v.) increased cortical ACh release, both in male and in female guinea pigs. In female guinea pigs chronically treated with 8-OHDPAT (0.1 mg/kg daily s.c., for 14 days) the facilitatory effect of 8-OHDPAT and 5-HT was maintained. In chronically 8-OHDPAT-treated male guinea pigs, 8-OHDPAT no longer modified ACh release, while 5-HT inhibited it. The inhibition was prevented by the 5-HT3 antagonist MDL 72222 1 mg/kg s.c. These results indicate that differences exist between male and female guinea pigs in the adaptive responses to prolonged treatment with the selective 5-HT1A agonist 8-OHDPAT.
authors.affiliation: Institute of Pharmacology, University of Ferrara, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Siniscalchi
authors.forename: I
authors.initials: I
authors.lastname: Badini
authors.forename: C
authors.initials: C
authors.lastname: Bianchi
authors.forename: L
authors.initials: L
authors.lastname: Beani
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000493:pharmacokinetics / Q000494:pharmacology*; D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D008297:Male; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D012727:Sex Characteristics; D014326:Tropanes / Q000494:pharmacology
chemical_list: D012702:Serotonin Antagonists; D014326:Tropanes; D012701:Serotonin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000109:Acetylcholine; C042253:bemesetron