[Protective effect of monoclonal antibody of tumor necrosis factor-alpha for vital organs in a model suffering from intestinal ischemia and reperfusion injury].
Monoclonal antibody to tumor necrosis factor-alpha (TNFa-MAb), z8, was used to explore protective effect on multiple organ dysfunction caused by intestinal ischemia and reperfusion in rats. Systemic plasma TNF level rose rapidly after release of the clamp, on superior mesenteric artery, and reached peak level 2 hours later. Endotoxemia and bacteremia were associated with systemic TNF level, and portal endotoxin concentration increased significantly before elevation of TNF activity. Pretreatment with anti-TNFa antibody markedly attenuated the increase of TNF level and provided protection from the development of hypotension, vital organ dysfunction, and metabolic acidosis. As a result the survival rate in treatment group increased by 35.7%. Our results demonstrated that TNF might play an important role in mediating the pathophysiologic changes in the pathogenesis of multiple organ damage in this intestinal ischemia-reperfusion injury model, and monoclonal antibody to TNF offered significant protection against multiple organ dysfunction or failure after severe trauma.
Yao
Y M
YM
Trauma Center, Postgraduate Medical College and 304 Hospital, People's Liberation Army, Beijing.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D007422:Intestines / Q000098:blood supply*; D007511:Ischemia / Q000150:complications*; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha