Detection of anthelmintic resistance by culture in vitro of parasitic stages of ovine nematodes.
Exsheathed L3 larvae of Haemonchus contortus were cultured at 38.5 degrees C in a complex medium supporting growth to adult worms. Although there was a clear difference in response between susceptible and benzimidazole/ivermectin/closantel resistant H. contortus using thiabendazole, the test failed to differentiate satisfactorily between the strains using ivermectin or closantel.
Small
A J
AJ
Central Veterinary Laboratory, New Haw, Weybridge, UK.
Coles
G C
GC
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology; D004351:Drug Resistance; D007559:Ivermectin / Q000494:pharmacology; D007814:Larva / Q000187:drug effects; D009348:Nematoda / Q000187:drug effects*; D009349:Nematode Infections / Q000469:parasitology / Q000662:veterinary; D012458:Salicylanilides / Q000494:pharmacology; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D013827:Thiabendazole / Q000494:pharmacology
D000871:Anthelmintics; D001562:Benzimidazoles; D012458:Salicylanilides; D007559:Ivermectin; C023342:closantel; D013827:Thiabendazole