A macrophage differentiating factor derived from human T cell line HUT102 acting on a mouse myeloid cell line M1.
Human T cell leukemia virus type I-transformed T cell line HUT102 constitutively secreted soluble factors which induced differentiation of a murine myeloid leukemic cell line, M1, to increase the immune complex-binding and/or phagocytizing capacity. This macrophage differentiating factor(s) (MDF) was purified from the culture supernatants of HUT102 cells by using several steps of column chromatography and novel immune-adherence and/or immune-phagocytic assays. The finally purified MDF activity was detected in the fraction that consisted of 40,000- and 45,000- molecular weight molecules. Antibodies specific for human interleukin-6 or for human granulocyte-colony stimulating factor, both of which have differentiation-inducing activity on M1 cells when used as a single factor, could not neutralize the MDF activity. These findings suggest that the 40,000- and/or 45,000- molecular weight molecules in the HUT102 cell products may be possible novel differentiation-inducing factors acting on a murine macrophage lineage across the species barrier.
Kanno
H
H
Department of Pathology, Tohoku University School of Medicine, Sendai.
Nose
M
M
Niki
T
T
Miyazawa
M
M
Kyogoku
M
M
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002461:Cell Line, Transformed; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003513:Cycloheximide / Q000494:pharmacology; D016207:Cytokines / Q000276:immunology; D015368:Human T-lymphotropic virus 1; D006801:Humans; D008222:Lymphokines / Q000302:isolation & purification* / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D014407:Tumor Cells, Cultured
D016207:Cytokines; D008222:Lymphokines; C050979:monocyte-macrophage differentiation factor; D003513:Cycloheximide