Effects of CD8 depletion on retinal soluble antigen induced experimental autoimmune uveoretinitis.
During the later stages of soluble-antigen (sAg)-induced experimental autoimmune uveoretinitis (EAU), an increase in the relative number of CD8+ lymphocytes has been observed at the site of inflammation in the retina. It has been suggested that these late-appearing CD8+ cells might down-regulate this acute disease process. To determine the role of the CD8+ cells in EAU, Lewis rats were depleted of CD8+ cells prior to and during disease and the enucleated eyes examined histologically. The spleen cells from CD8-depleted rats were also examined for their ability to respond to concanavalin A (Con A) and to allogeneic targets as determined by mixed lymphocyte reaction (MLR) and cytotoxicity assays. The results suggest that depleting CD8+ cells had no effect on the course of disease and that CD8+ cells do not play a crucial role in the immunoregulation of EAU.
Calder
V L
VL
Dept. of Clinical Science, Institute of Ophthalmology, London, U.K.
Zhao
Z S
ZS
Wang
Y
Y
Barton
K
K
Lightman
S L
SL
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens / Q000032:analysis*; D003208:Concanavalin A / Q000276:immunology; D005123:Eye / Q000473:pathology; D005260:Female; D007124:Immunoenzyme Techniques; D007959:Lymphocyte Culture Test, Mixed; D051381:Rats; D011917:Rats, Inbred Lew; D012173:Retinitis / Q000276:immunology* / Q000473:pathology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014605:Uveitis / Q000276:immunology* / Q000473:pathology
D016827:CD8 Antigens; D003208:Concanavalin A