[A combined staining of immunohistochemistry and in situ hybridization for detecting epidemic hemorrhagic fever virus in tissue section].
A combined staining of immunohistochemistry and in situ hybridization has been successfully applied to detect antigen and viral RNA at the same tissue slice from epidemic hemorrhagic fever (EHF) autopsy. Multiple McAb were used to detect EHFV antigen, followed with a digoxigenin-labelling cDNA probe for detecting EHFV RNA respectively. The viral antigen was seen in the cellular cytoplasm and viral RNA was demonstrated within the nuclei. It is emphasized that pretreatment of the section with Lugol's solution and choice of suitable proteolytic enzyme as well as proper temperature and time for the hybridization are the key points for the staining.
Tang
Z J
ZJ
Department of Pathology, Hubei Medical College, Wuhan.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000382:microbiology; D015342:DNA Probes; D006476:Orthohantavirus / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000382:microbiology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D012367:RNA, Viral / Q000032:analysis
D000956:Antigens, Viral; D015342:DNA Probes; D012367:RNA, Viral