Iron- and lactic acid-induced lipid peroxidation in rat kidney homogenates and slices.
Lactic acid 4 mM acted as a lipoperoxidant by increasing production of thiobarbituric acid-reactive substances (TBARS) in rat kidney slices and homogenates. This effect occurred mainly when slices and homogenates were incubated in a pH 5.4 medium conductive to full expression of compound acidity. TBARS increase was only slight when incubation was performed in Krebs buffer, pH 7.4. Moreover, sodium lactate 4 mM increased TBARS production only when homogenates were incubated in the pH 5.4 medium. Deferoxamine (1 mM) inhibited the prooxidant effect of lactic acid 4 mM, and TBARS increase was correlated with iron release. The iron mobilized may come from reserves where it is weakly bound or from ferritin; and ascorbic acid, present in low quantities in kidney, might trigger the release of this product.
Fauconneau
B
B
Institute of Xenobiotic Studies, Faculty of Medicine and Pharmacy, Poitiers, France.
Tallineau
C
C
Huguet
F
F
Guillard
O
O
Piriou
A
A
D000818:Animals; D003676:Deferoxamine / Q000494:pharmacology; D005293:Ferritins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007773:Lactates / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D019344:Lactic Acid; D015227:Lipid Peroxidation / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis*
D007773:Lactates; D017392:Thiobarbituric Acid Reactive Substances; D019344:Lactic Acid; D005293:Ferritins; D007501:Iron; D003676:Deferoxamine