DNA ligase I is associated with the 21 S complex of enzymes for DNA synthesis in HeLa cells.
Approximately 80% of the DNA ligase activity in HeLa cell extracts is associated with the 21 S enzyme complex that functions in simian virus 40 DNA replication in vitro (Malkas et al., Biochemistry 29, 6362-6374., 1990). The DNA ligase associated with the 21 S complex was purified extensively and its physical, enzymic and immunological properties characterized as DNA ligase I. The association of DNA ligase I with the 21 S complex of enzymes for DNA synthesis provides evidence for the physiological function of this DNA ligase in DNA replication in human cells.
Li
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Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545.
Goodchild
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Baril
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D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000378:metabolism*; D004261:DNA Replication / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism*; D013539:Simian virus 40 / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics
D004279:DNA, Viral; C000607892:LIG1 protein, human; D009097:Multienzyme Complexes; D011088:DNA Ligases; D000072481:DNA Ligase ATP