Cloning of cmpE, a plasmid-borne catechol 2,3-dioxygenase-encoding gene from the aromatic- and chloroaromatic-degrading Pseudomonas sp. HV3.
Pseudomonas sp. strain HV3 degrades aromatics and chloroaromatics. It harbours a mega-plasmid, designated pSKY4, from which the gene cmpE, encoding a catechol 2,3-dioxygenase (C23O) catalyzing the conversion of catechol to 2-hydroxymuconic semialdehyde, was cloned and sequenced. The deduced amino acid (aa) sequence shows the highest homology, 52%, to the deduced aa sequences of xylE1 and dmpB. The deduced 307-aa sequence of cmpE contains the extradiol ring-cleavage signature in the same position as other 307-aa C23O-encoding genes.
Yrjälä
K
K
Department of General Microbiology, University of Helsinki, Finland.
Paulin
L
L
Kilpi
S
S
Romantschuk
M
M
D000595:Amino Acid Sequence; D050557:Catechol 2,3-Dioxygenase; D003001:Cloning, Molecular / Q000379:methods; D049308:Dioxygenases; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000096:biosynthesis* / Q000235:genetics*; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase