Impaired human tissue factor-mediated activity in blood clotting factor VIINagoya (Arg304-->Trp). Evidence that a region in the catalytic domain of factor VII is important for the association with tissue factor.
A 37-year-old man was found to have an inherited abnormal factor VII (FVII) (designated FVIINagoya) that has a lower FVII procoagulant activity when tested in a one-stage assay using rabbit, simian, or human tissue factor (TF), but surprisingly has a nearly normal activity using bovine TF. DNA sequence analysis of the propositus' FVII gene revealed a C to T mutation that results in a novel amino acid substitution of Arg304 to Trp. Restriction enzyme analysis of the amplified fragment obtained from each family member demonstrated that the patient is homozygous for the point mutation, and the mother and sister of the patient are heterozygous for this mutation. To elucidate the abnormality in FVIINagoya, we compared the recombinant mutant FVII with normal FVII. In the presence of human TF, kinetic analyses demonstrated that the apparent Km values of FVIINagoya for factor Xa-mediated FVII activation and for FVIIa-mediated factor X (FX) activation were 3.6- and 4.5-fold higher than those of normal FVII, respectively. The ligand binding assay to human TF also showed that FVIINagoya had a 5.2-fold larger apparent Kd than normal. In the presence of bovine TF, however, these values showed no difference between normal and mutant FVII. These findings indicate that the major abnormality in FVIINagoya is not a catalytic dysfunction but an impaired complex-formation of human TF.FVII.FX, suggesting the importance of Arg304 in the interaction with human TF.
Matsushita
T
T
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Kojima
T
T
Emi
N
N
Takahashi
I
I
Saito
H
H
D000328:Adult; D000818:Animals; D001120:Arginine / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D001777:Blood Coagulation; D002384:Catalysis; D002417:Cattle; D017931:DNA Primers; D005167:Factor VII / Q000235:genetics / Q000378:metabolism*; D005170:Factor X / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013925:Thromboplastin / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism
D017931:DNA Primers; D011994:Recombinant Proteins; D014364:Tryptophan; D005167:Factor VII; D005170:Factor X; D013925:Thromboplastin; D001120:Arginine