Terminal deoxynucleotidyl transferase and glucocorticoid receptors in rat thymocytes.
Higher level of terminal deoxynucleotidyl transferase (TdT) and peanut agglutinin (PNA)-positive cells were found in those fractions of albumin gradient separated rat thymocytes that had a higher level of glucocorticoid (GC) receptors and a lower level of sialyltransferase (S-T) activity. Incubation of thymocytes in these fractions with dexamethasone inhibited RNA synthesis significantly. Incubation of these thymocytes with tetradecanoylphorbol-acetate (TPA) reduced TdT activity, while increasing S-T activity. The higher level of GC receptors may be responsible for GC sensitivity of TdT-positive thymocytes. In addition, sialyltransferase may be a biochemical marker for thymocyte differentiation.
Sasaki
R
R
Department of Haematology, Jichi Medical School, Tochigi, Japan.
Takaku
F
F
Bollum
F J
FJ
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D004253:DNA Nucleotidylexotransferase / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D003907:Dexamethasone / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D007106:Immune Sera; D008297:Male; D009696:Nucleic Acids / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000032:analysis*; D011975:Receptors, Mitogen / Q000032:analysis; D012799:Sialyltransferases / Q000032:analysis*; D013601:T-Lymphocytes / Q000737:chemistry* / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013950:Thymus Gland / Q000737:chemistry / Q000166:cytology*
D015415:Biomarkers; D007106:Immune Sera; D009696:Nucleic Acids; D011965:Receptors, Glucocorticoid; D011975:Receptors, Mitogen; C028697:dexamethasone receptor; C038223:peanut agglutinin receptor; D003907:Dexamethasone; D012799:Sialyltransferases; D004253:DNA Nucleotidylexotransferase; D013755:Tetradecanoylphorbol Acetate