title: Nucleotide sequence and organization of eight 3' open reading frames of the citrus tristeza closterovirus genome.
abstract: The citrus tristeza closterovirus (CTV) RNA genome was cloned as cDNA generated from both CTV-specific double-stranded RNA and genomic RNA, and the sequence of the 3' 7292 nucleotides was determined. The sequenced portion contained eight open reading frames potentially encoding, in the 5' to 3' direction, proteins with the apparent molecular weights of 65, 61, 27, 25 (capsid protein, CP), 18, 13, 20, and 23 kDa, and a potential noncoding region of 277 nucleotides. The 65-kDa protein is a viral homolog of cellular hsp70 heat shock proteins (hsp), the 61-kDa protein is distantly related to the hsp90 proteins, and the 27-kDa protein is a diverged copy of the CP. Database searches did not identify any protein sequences of significant similarity to the remaining four ORFs downstream of the CP. A specific four-gene module consisting of the hsp70 protein, the hsp90-related protein, the diverged copy of the CP, and the CP itself was found to be common in organization between CTV and beet yellows closterovirus. All four proteins in this module were highly conserved, indicating that these viruses probably have evolved from a common ancestor.
authors.affiliation: Plant Pathology Department, University of Florida, Gainesville 32611.
authors.forename: H R
authors.initials: HR
authors.lastname: Pappu
authors.forename: A V
authors.initials: AV
authors.lastname: Karasev
authors.forename: E J
authors.initials: EJ
authors.lastname: Anderson
authors.forename: S S
authors.initials: SS
authors.lastname: Pappu
authors.forename: M E
authors.initials: ME
authors.lastname: Hilf
authors.forename: V J
authors.initials: VJ
authors.lastname: Febres
authors.forename: R M
authors.initials: RM
authors.lastname: Eckloff
authors.forename: M
authors.initials: M
authors.lastname: McCaffery
authors.forename: V
authors.initials: V
authors.lastname: Boyko
authors.forename: S
authors.initials: S
authors.lastname: Gowda
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017790:Closterovirus / Q000235:genetics*; D004279:DNA, Viral; D016679:Genome, Viral; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins