title: Use of solid-phase PCR for enhanced detection of airborne microorganisms.
abstract: The solid-phase PCR (SP-PCR) was compared with a culture-based technique for the detection of aerosolized Escherichia coli DH1. Results with SP-PCR showed an increase in detection sensitivity over that of culture methods. Therefore, SP-PCR may be useful for the detection of airborne microorganisms which may be nonculturable because of aerosolization or sampling stress.
authors.affiliation: Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras 00931.
authors.forename: A J
authors.initials: AJ
authors.lastname: Alvarez
authors.forename: M P
authors.initials: MP
authors.lastname: Buttner
authors.forename: G A
authors.initials: GA
authors.lastname: Toranzos
authors.forename: E A
authors.initials: EA
authors.lastname: Dvorsky
authors.forename: A
authors.initials: A
authors.lastname: Toro
authors.forename: T B
authors.initials: TB
authors.lastname: Heikes
authors.forename: L E
authors.initials: LE
authors.lastname: Mertikas-Pifer
authors.forename: L D
authors.initials: LD
authors.lastname: Stetzenbach
mesh_terms: D000391:Air Microbiology; D001483:Base Sequence; D015169:Colony Count, Microbial; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial