Atherosclerosis-associated changes in the carbohydrate-binding capacities of smooth muscle cells of various human arteries.
A set of labelled neoglycoproteins and sulfated polysaccharides, recognizing carbohydrate receptors specific for glucose (glu), mannose (man), lactose (lac), fucose (fuc), fucoidan (fud), dextran sulfate (dex), and heparin (hep), as well as polyclonal antibodies specific for an endogenous beta-galactoside-specific lectin of molecular weight 14kD (A14kD) and a heparin-binding lectin (AHL) have been applied to the main arteries of 10 autopsy cases. Slides of the following types of vessels were incubated with solutions of the biotinylated probes or antibodies at room temperature for 60 min: right and left coronary artery, carotid artery, abdominal and thoracic aorta, pulmonal artery, and the left femoral artery. Atherosclerotic lesions and non-atherosclerotic areas were analyzed for each individual type of vessel. The percentage of the determined expression of the presence of specific binding sites for the various probes was the lowest in the carotid and cardiac arteries, and the highest in the pulmonal artery. Pronounced quantitative differences between the normal and atherosclerotic arterial walls were noted for binding of fuc-, man-, and lac-exposing neoglyco-proteins of the right coronary artery and the carotid artery. Pulmonal and femoral arteries differed with respect to fucoidan or dextran sulfate binding. The heparin-specific lectin and the 14kD-lectin were found to be present in nearly all arterial walls, independent from the localization and the presence of an atherosclerotic lesion. The findings suggest that the expression of sugar receptors, as assessed by labelled neoglycoproteins or sulfated polysaccharides, may be of importance in the development of atherosclerotic lesions in the coronary and carotid arteries.
Kayser
K
K
Department of Pathology, Thoraxklinik, Heidelberg, Germany.
Bartels
S
S
Yoshida
Y
Y
Fernandez-Britto
J
J
Gabius
H J
HJ
D000368:Aged; D000369:Aged, 80 and over; D000941:Antigens / Q000032:analysis / Q000096:biosynthesis; D001158:Arteries / Q000378:metabolism / Q000473:pathology*; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology*; D050260:Carbohydrate Metabolism; D002339:Carotid Arteries / Q000378:metabolism / Q000473:pathology; D003331:Coronary Vessels / Q000378:metabolism / Q000473:pathology; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D037102:Lectins / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology*
D000941:Antigens; D006023:Glycoproteins; D037102:Lectins