Identification and characterization of a Candida albicans-binding proteoglycan secreted from rat submandibular salivary glands.
A previously identified Candida albicans-binding glycoprotein secreted from rat submandibular glands (RSMG) has been further purified from an aqueous RSMG extract by ion-exchange chromatography and gel filtration. Biochemical analysis of the glycoprotein revealed high levels of uronic acid and sulfate, suggesting that it was a proteoglycan. Its amino acid and carbohydrate compositions were similar to those observed for other proteoglycans and differed significantly from those of RSMG mucin, the major secretory glycoprotein of RSMG. In addition, the apparent molecular weight of the glycoprotein was reduced following treatment with either chondroitinase ABC or heparitinase, demonstrating the presence of chondroitin sulfate and heparan sulfate. On the basis of its structure and anatomical source, the glycoprotein is referred to as submandibular gland secreted proteoglycan 1 (SGSP1). SGSP1 also binds monoclonal antibody 1F9, which recognizes the human blood group A carbohydrate epitope found on RSMG mucin. Hence, SGSP1 appears to be a hybrid molecule with carbohydrate structures found in both proteoglycans and RSMG mucin. Enzymatic digestion of SGSP1, followed by its interaction with a radiolabelled C. albicans strain in a filter-binding assay, demonstrated that binding to this strain appears to be mediated primarily via the heparan sulfate side chains of SGSP1 and not via the blood group A oligosaccharide.
Hoffman
M P
MP
Department of Microbiology & Immunology, University of Rochester School of Medicine and Dentistry, New York 14642.
Haidaris
C G
CG
D000818:Animals; D002176:Candida albicans / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006025:Glycosaminoglycans / Q000032:analysis; D008297:Male; D011509:Proteoglycans / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D051381:Rats; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000737:chemistry*
D006025:Glycosaminoglycans; D011509:Proteoglycans