title: Induction of the marsupial sperm acrosome reaction in vitro by treatment with diacylglycerols.
abstract: The diacylglycerol DiC8 (1,2-dioctanoyl-sn-glycerol; 100 mumol l-1) was found to induce acrosomal loss in 70% of wallaby spermatozoa and 40% of possum spermatozoa after incubation for 120 min. If 10 mumol calcium ionophore l-1 was present, the time required to reach these end points was reduced to 30 and 60 min, respectively. The diacylglycerol OAG (1-oleoyl-2-acetyl-sn-glycerol; 100 mumol l-1) produced some acrosomal loss, particularly when 10 mumol calcium ionophore l-1 was present, but when the ionophore was absent, results were equivocal. The other diacylglycerol tested DOG (1,2-dioleoyl-sn-glycerol; 100 mumol l-1) did not induce significant acrosomal loss in possum or wallaby spermatozoa. The concentration of DiC8 required to induce acrosomal loss in marsupial spermatozoa (50-100 mumol l-1) was relatively high compared with that for placental mammals, suggesting a less specific mode of action than enzyme activation. Of the diacylglycerols tested alone, only DiC8 led to significant loss of motility. In wallabies, this was detectable after incubation for 60 min and in possums after incubation for 120 min. The ultrastructure of the DiC8-induced acrosomal loss was essentially identical to the acrosome reaction described for a broad range of placental mammals. A membrane vesicle shroud covered the acrosomal surface of the sperm head. The vesicles appeared to be formed by multiple point fusions between the plasma membrane and the outer acrosomal membrane. The mechanism of DiC8-induced acrosomal loss remains to be established.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biological Sciences, University of Newcastle, NSW, Australia.
authors.forename: Y
authors.initials: Y
authors.lastname: Sistina
authors.forename: M
authors.initials: M
authors.lastname: Lin
authors.forename: K E
authors.initials: KE
authors.lastname: Mate
authors.forename: J C
authors.initials: JC
authors.lastname: Rodger
mesh_terms: D000177:Acrosome / Q000187:drug effects* / Q000648:ultrastructure; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D004075:Diglycerides / Q000494:pharmacology*; D005089:Exocytosis / Q000187:drug effects*; D007614:Macropodidae / Q000502:physiology; D008297:Male; D008394:Marsupialia / Q000502:physiology*; D008854:Microscopy, Electron; D013081:Sperm Motility / Q000187:drug effects
chemical_list: D004075:Diglycerides; C044295:1,2-dioctanoylglycerol; D000001:Calcimycin; C038237:1-oleoyl-2-acetylglycerol; C013965:diolein