title: The effect of insulin on proteins associated with free, cytoskeletal-bound and membrane-bound polysome populations.
abstract: It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal-bound (CBP) and membrane-bound polysome (MBP) populations. This paper concerns an analysis of the pattern of proteins in high-salt extracts of FP, CBP and MBP isolated from Krebs II ascites and MPC-11 cells. A combined detergent/salt extraction procedure was used to isolate the three fractions of polysomes from control cells and from cells following short-term stimulation with insulin. There were differences in the protein patterns in the individual fractions and changes occurred after insulin stimulation.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bergen, Norway.
authors.forename: R
authors.initials: R
authors.lastname: Moss
authors.forename: I F
authors.initials: IF
authors.lastname: Pryme
authors.forename: A
authors.initials: A
authors.lastname: Vedeler
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D003599:Cytoskeleton / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D007328:Insulin / Q000494:pharmacology*; D008970:Molecular Weight; D011132:Polyribosomes / Q000737:chemistry* / Q000187:drug effects; D011506:Proteins / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D007328:Insulin; D011506:Proteins