Cytokine combinations increase p75 tumor necrosis factor receptor binding and stimulate receptor shedding in rheumatoid synovial fibroblasts.
OBJECTIVE
To identify cytokines responsible for the increased levels of tumor necrosis factor receptor (TNFR) in the joints of patients with rheumatoid arthritis.


METHODS
Antibodies to TNFR types were used both to inhibit ligand cell binding and to quantify released receptors in rheumatoid synovial fibroblasts.


RESULTS
Binding by and shedding of the p75 TNFR was affected by interleukin-1 (IL-1), IL-4, and interferon-gamma.


CONCLUSIONS
IL-1 could cause increased TNF alpha binding and TNFR shedding in inflamed joints.
Taylor
D J
DJ
University Department of Medicine, University Hospital of South Manchester, West Didsbury, England.
D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D016207:Cytokines / Q000494:pharmacology*; D004338:Drug Combinations; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D018124:Receptors, Tumor Necrosis Factor / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D004338:Drug Combinations; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha