Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR.
A single-tube nested PCR able to discriminate between ocular infection with Chlamydia trachomatis of serovar A, B, or C is described. The method uses genotype-specific primers labeled with different fluorochromes, "drop-in/drop-out" PCR amplification, and product analysis on an Applied Biosystems 373A DNA Sequencer using GENESCAN 672 software. The system readily detected mixed infection with serotypes A and B within the same eye. This strategy provides a rapid genotyping method applicable to a wide variety of epidemiological studies.
Pecharatana
S
S
Department of Molecular Microbiology, University Medical School, Southampton General Hospital, UK.
Pickett
M A
MA
Watt
P J
PJ
Ward
M E
ME
D001483:Base Sequence; D002690:Chlamydia Infections / Q000453:epidemiology / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D015818:Eye Infections, Bacterial / Q000453:epidemiology / Q000382:microbiology*; D005456:Fluorescent Dyes; D005714:Gambia / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D005456:Fluorescent Dyes