Human fetal membrane contribution to the prevention of parturition: uterotonin degradation.
This investigation was conducted to evaluate the potential capacity of the human fetal membranes-decidua parietalis, and in particular the chorion laeve, to degrade uterotonins that are produced in amnion, are present in amniotic fluid, or both. The four uterotonins that have been evaluated most frequently as myometrial contractants potentially involved in the initiation of human parturition are prostaglandins, oxytocin, endothelin-1, and platelet-activating factor. We assessed the levels of mRNA and the specific activities (SAs) of enkephalinase (the plasma membrane endopeptidase that degrades endothelins) and prostaglandin dehydrogenase (PGDH) in human fetal membranes, i.e. amnion and chorion leave, and in decidua parietalis. The SA of oxytocinase (which inactivates oxytocin) in these tissues also was determined. The SA of enkephalinase in chorion laeve from all anatomical sites (singleton and diamnionic-dichorionic twin placentae) in all pregnancies studied (mean +/- SEM, 95 +/- 7.9 ng/min.mg protein; n = 28) is similar to that in human fetal kidney (89.5 +/- 2.8; n = 6). Kidney tissue is believed to be one of the richest sources of enkephalinase. The SAs of enkephalinase in amnion (18.3 +/- 2.3 nmol/min.mg protein; n = 29) and in decidua parietalis (31.8 +/- 6.7; n = 20) also were high, but significantly less than that in chorion leave. The level of enkephalinase mRNA in chorion laeve in singleton pregnancies is high, as is the SA of enkephalinase (111.9 +/- 10.6 nmol/min.mg protein; n = 17). In paired chorion laeve tissues from five diamnionic-dichorionic twin placentae, the SAs of enkephalinase in reflected chorion laeve (74 +/- 12.8; P < 0.06 compared with singletons) and fused chorion laeve (64.8 +/- 6.5; P < 0.001 compared with singletons) were similar. The SA of PGDH in reflected chorion leave (46.3 +/- 6.9 nmol/min.mg protein; n = 19) was significantly greater than that in decidua (16 +/- 5.5; n = 15). There was a significant correlation between the levels of PGDH mRNA and PGDH enzyme SA. In fused chorion laeve of diamnionic-dichorionic twin placentae, the SA of PGDH (14.9 +/- 7.3; n = 4) was much less than that in reflected chorion laeve of the same twin pregnancy (70.5 +/- 14.7; n = 4). PGDH mRNA was not detectable in amnion tissue (n = 5) by northern analysis, and the SA of PGDH (< 1.2 +/- 1.0; n = 6) in amnion was undetectable or near the lower limit of assay detection.(ABSTRACT TRUNCATED AT 400 WORDS)
Germain
A M
AM
Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas, Southwestern Medical Center, Dallas 75235-9051.
Smith
J
J
Casey
M L
ML
MacDonald
P C
PC
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000650:Amnion / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D001483:Base Sequence; D002823:Chorion / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D010122:Cystinyl Aminopeptidase / Q000032:analysis / Q000235:genetics / Q000502:physiology; D003656:Decidua / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D016232:Endothelins / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D005321:Extraembryonic Membranes / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000032:analysis / Q000235:genetics / Q000502:physiology; D007743:Labor, Obstetric / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D015260:Neprilysin / Q000032:analysis / Q000235:genetics / Q000502:physiology; D010121:Oxytocin / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D010741:Phospholipases A / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D010972:Platelet Activating Factor / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D011247:Pregnancy; D011453:Prostaglandins / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
D016232:Endothelins; D010972:Platelet Activating Factor; D011453:Prostaglandins; D012333:RNA, Messenger; D010121:Oxytocin; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D010122:Cystinyl Aminopeptidase; D015260:Neprilysin