title: A comparison of immunoglobulin G-containing high-molecular-weight complexes isolated from children with juvenile rheumatoid arthritis and congenital human immunodeficiency virus infection.
abstract: Circulating immune complexes have been described in both juvenile rheumatoid arthritis and in AIDS. We isolated high-molecular-weight complexes from plasma of children with juvenile rheumatoid arthritis and congenital human immunodeficiency virus infection by sequential gel filtration followed by affinity chromatography on protein A-Sepharose. We demonstrate that 1) mixed IgG-, IgM-, and IgA-bearing immune complexes can be found in both juvenile rheumatoid arthritis and congenital human immunodeficiency virus infection and 2) complexes isolated via affinity chromatography on protein A-Sepharose do not have detectable C4 but activate the classic complement pathway in vitro.
authors.affiliation: Division of Immunology/Rheumatology, Wayne State University School of Medicine, Detroit, Michigan 48201.
authors.forename: J N
authors.initials: JN
authors.lastname: Jarvis
authors.forename: M
authors.initials: M
authors.lastname: Iobidze
authors.forename: H
authors.initials: H
authors.lastname: Taylor
authors.forename: J
authors.initials: J
authors.lastname: DeJonge
authors.forename: S
authors.initials: S
authors.lastname: Chang
mesh_terms: D000293:Adolescent; D000936:Antigen-Antibody Complex / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D001171:Arthritis, Juvenile / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity; D003181:Complement C4 / Q000378:metabolism; D015935:Complement C4b; D003171:Complement Pathway, Classical; D015658:HIV Infections / Q000151:congenital / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood; D066298:In Vitro Techniques; D007223:Infant; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism; D012217:Rheumatoid Factor / Q000097:blood
chemical_list: D000936:Antigen-Antibody Complex; D003181:Complement C4; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010446:Peptide Fragments; D015935:Complement C4b; C032261:complement C4d; D012217:Rheumatoid Factor