Reactive oxygen species produced in metal-catalyzed oxidation of bis(trifluoromethyl)disulfide and protection by ZE.
Bis(trifluoromethyl)disulfide (TFD), used as an industrial fumigant, was found to generate a thiyl free radical as seen by EPR/spin trapping. Oxygen appears to be an absolute requirement for radical production. The results obtained in this investigation implicate the production of thiyl and reactive oxygen species (ROS), superoxide radical anion and hydroxyl radicals, during TFD autoxidation. The rate of production of these free radical intermediates was found to increase in the presence of iron(III) and copper(II). In addition, the metal ion chelator DETAPAC and ROS scavengers ethanol, mannitol, and PEG-SOD/catalase were found to inhibit free radical production. Reactive oxygen species were not formed when a high-potency zinc plus antioxidant, ZE caps, was present. These results provide support for the pro-oxidation of TFD and a protective role for zinc.
Arroyo
C M
CM
U.S. Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, Maryland 21010-5425.
Kirby
S D
SD
Werrlein
R J
RJ
McCarthy
R L
RL
Moran
T S
TS
keeler
J R
JR
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002417:Cattle; D004220:Disulfides / Q000737:chemistry*; D004347:Drug Interactions; D004578:Electron Spin Resonance Spectroscopy; D005466:Fluorocarbons / Q000737:chemistry*; D016166:Free Radical Scavengers; D005942:Gluconates / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D004220:Disulfides; D005466:Fluorocarbons; D016166:Free Radical Scavengers; D005942:Gluconates; D017382:Reactive Oxygen Species; D014810:Vitamin E; C074419:bis(trifluoromethyl)disulfide; D011092:Polyethylene Glycols; D013482:Superoxide Dismutase; C063471:polyethylene glycol-superoxide dismutase; C030691:gluconic acid