title: Induction of immediate early genes by cyclic AMP in primary cultures of neurons from rat cerebral cortex.
abstract: In this paper, we tested whether physiological activators of the cAMP second messenger pathway in primary cultures of neurons from rat cerebral cortex directly induce c-fos and other immediate early gene (IEG) transcription factors. We have found that brief (30 s to 2 min) stimulation of neurons with vasoactive intestinal peptide (VIP) and SKF-38393, a D1-dopaminergic receptor agonist, potently increased mRNA levels for the IEGs c-fos, jun-B, and NGFI-A, with weaker increases for c-jun. This action was mimicked by forskolin and dibutyryl cAMP. IEG induction by VIP and dibutyryl cAMP was not blocked by excitatory amino acid receptor antagonists or by blockers of dihydropyridine-sensitive calcium channels. Moreover, calcium-free medium did not modify IEG induction by dibutyryl cAMP, suggesting that cAMP can directly regulate IEG expression in differentiated neurons independently of calcium.
authors.affiliation: Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06508.
authors.forename: F M
authors.initials: FM
authors.lastname: Vaccarino
authors.forename: M D
authors.initials: MD
authors.lastname: Hayward
authors.forename: H N
authors.initials: HN
authors.lastname: Le
authors.forename: D J
authors.initials: DJ
authors.lastname: Hartigan
authors.forename: R S
authors.initials: RS
authors.lastname: Duman
authors.forename: E J
authors.initials: EJ
authors.lastname: Nestler
mesh_terms: D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D015259:Dopamine Agents / Q000494:pharmacology; D051766:Early Growth Response Protein 1; D004873:Ergolines / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D016762:Genes, fos; D016758:Genes, jun; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D017874:Immediate-Early Proteins; D007150:Immunohistochemistry; D007700:Kinetics; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D019257:Quinpirole; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D015290:Second Messenger Systems; D014157:Transcription Factors / Q000096:biosynthesis*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
chemical_list: D004268:DNA-Binding Proteins; D015259:Dopamine Agents; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D004873:Ergolines; D005971:Glutamates; D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D005576:Colforsin; D019257:Quinpirole; D014660:Vasoactive Intestinal Peptide; D018698:Glutamic Acid; D003994:Bucladesine; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; D000242:Cyclic AMP