title: Preliminary crystallographic analysis of methane mono-oxygenase hydroxylase from Methylosinus trichosporium OB3b.
abstract: The hydroxylase component of the enzyme methane mono-oxygenase from Methylosinus trichosporium OB3b has been crystallized in the orthorhombic space group C222(1) with unit cell dimensions a = 264.5 A, b = 71.2 A, c = 139.4 A. The crystals grow as square, thick plates and diffract to beyond 2 A resolution. There is one half of the hydroxylase dimer in the asymmetric unit.
authors.affiliation: Department of Biochemistry, University of Minnesota Medical School, Minneapolis 55455.
authors.forename: W A
authors.initials: WA
authors.lastname: Froland
authors.forename: D H
authors.initials: DH
authors.lastname: Dyer
authors.forename: R
authors.initials: R
authors.lastname: Radhakrishnan
authors.forename: C A
authors.initials: CA
authors.lastname: Earhart
authors.forename: J D
authors.initials: JD
authors.lastname: Lipscomb
authors.forename: D H
authors.initials: DH
authors.lastname: Ohlendorf
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D008772:Methylococcaceae / Q000201:enzymology*; D010105:Oxygenases / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation
chemical_list: D010105:Oxygenases; C021555:methane monooxygenase