title: Restriction end-converting vectors with tandem repeated multiple cloning sites.
abstract: The construction, characterization and uses of four vectors with novel multiple cloning sites (MCS) are described. The MCS consist of a central zone with unique restriction sites, flanked by two zones in which four sites are repeated in a direct sense. These vectors allow the insertion of fragments with the same or different restriction ends and their rescue with a multiple combination of ends, identical or different. This system conserves the XGal blue-white selection characteristics of the pUC plasmids, as well as their use as sequencing vehicles.
authors.affiliation: Centro Nacional de Biotecnología, Cantoblanco, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: DeLaFuente
authors.forename: E
authors.initials: E
authors.lastname: Cisneros
authors.forename: A
authors.initials: A
authors.lastname: Talavera
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase