title: Ultrastructural localization of neurotensin immunoreactivity in the stellate ganglion of the cat.
abstract: The morphological features and cellular relationships of neurotensin-containing axon terminals were studied at light and electron microscopic levels in the cat stellate ganglion using peroxidase and immunogold immunocytochemistry. By light microscopy, neurotensin immunoreactivity was detected within thin varicose fibres distributed throughout the ganglion. Immunoreactive fibres were no longer apparent following chronic deafferentation of the ganglion indicating that they were of extrinsic origin. Ultrastructural analysis of peroxidase immunostained material confirmed the presence of neurotensin immunoreactivity within a subpopulation of axonal varicosities which made synaptic contacts with the dendrites of ganglion cells. Within labelled varicosities neurotensin immunoreactivity was found by both immunoperoxidase and immunogold methods to be concentrated within large dense core vesicles 80-120 nm in diameter. These large dense core vesicles were characteristically distant from the active zone, in keeping with a possible extrasynaptic release of the peptide.
authors.affiliation: Department of Pharmacology, McGill University, Montreal, Quebec, Canada.
authors.forename: M A
authors.initials: MA
authors.lastname: Morales
authors.forename: M
authors.initials: M
authors.lastname: Bachoo
authors.forename: A
authors.initials: A
authors.lastname: Beaudet
authors.forename: B
authors.initials: B
authors.lastname: Collier
authors.forename: C
authors.initials: C
authors.lastname: Polosa
mesh_terms: D000818:Animals; D001369:Axons / Q000737:chemistry; D002415:Cats; D005260:Female; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009496:Neurotensin / Q000032:analysis*; D013233:Stellate Ganglion / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D009496:Neurotensin