title: Induction of beta interferon by human immunodeficiency virus type 1 and its gp120 protein in human monocytes-macrophages: role of beta interferon in restriction of virus replication.
abstract: In vitro cultivated human monocytes show a time-dependent differentiation into macrophages, characterized by an increased expression of macrophage-specific antigens. Monocytes-macrophages were infected with human immunodeficiency virus type 1 strain Ba-L (HIV-1Ba-L) at different stages of differentiation. When 7-day cultured macrophages were infected in the presence of antibodies to beta interferon (IFN-beta), a significant increase in HIV-1 p24 release was detected. This effect was not observed in 1-day monocytes. This finding suggests that IFN-beta secreted by the infected macrophages inhibits p24 release. Treatment of cultured macrophages with recombinant gp120 (rgp120) protein resulted in the induction of IFN-beta mRNA and in an antiviral state to vesicular stomatitis virus. This rgp120-induced antiviral state was largely neutralized by antibodies to IFN-beta, whereas anti-IFN-alpha antibodies were ineffective. In cultured macrophages, 0.1 IU of IFN-beta per ml was sufficient to induce a marked inhibition of vesicular stomatitis virus yield, whereas this dose was ineffective in 1-day monocytes. These results indicate that (i) HIV-1 (possibly in part through its gp120 protein) induces low levels of IFN-beta in macrophages and (ii) this IFN-beta is very effective in inducing an antiviral state in differentiated macrophages.
authors.affiliation: Department of Virology, Istituto Superiore di Sanita, Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Gessani
authors.forename: P
authors.initials: P
authors.lastname: Puddu
authors.forename: B
authors.initials: B
authors.lastname: Varano
authors.forename: P
authors.initials: P
authors.lastname: Borghi
authors.forename: L
authors.initials: L
authors.lastname: Conti
authors.forename: L
authors.initials: L
authors.lastname: Fantuzzi
authors.forename: F
authors.initials: F
authors.lastname: Belardelli
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015699:HIV Envelope Protein gp120 / Q000494:pharmacology*; D015497:HIV-1 / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D016899:Interferon-beta / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology / Q000502:physiology*; D009000:Monocytes / Q000187:drug effects / Q000382:microbiology / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D014779:Virus Replication
chemical_list: D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120; D007369:Interferon Inducers; D012333:RNA, Messenger; D016899:Interferon-beta