Immobilized phenylboronic acids for the selective extraction of beta-blocking drugs from aqueous solution and plasma.
The use of phenylboronic acid (PBA) solid-phase extraction cartridges for the extraction of a range of beta-blockers from aqueous buffer and plasma has been investigated and compared with other phases commonly used for solid-phase extraction. PBA was found to provide an efficient means for the extraction of this class of compound from simple aqueous buffer systems. Extraction from buffers was pH-dependent and gave optimum results at approximately pH 8. Extraction from plasma was less efficient for some of the test compounds; this matrix effect was probably due to protein binding. Chromatography showed that plasma extracts were free of major sources of interference.
Martin
P
P
Department of Safety of Medicines, ICI Pharmaceuticals, Alderly Park, Macclesfield, UK.
Leadbetter
B
B
Wilson
I D
ID
D000319:Adrenergic beta-Antagonists / Q000097:blood / Q000302:isolation & purification*; D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002021:Buffers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011433:Propranolol / Q000097:blood / Q000302:isolation & purification; D011485:Protein Binding; D051381:Rats; D058428:Silica Gel; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
D000319:Adrenergic beta-Antagonists; D001897:Boronic Acids; D002021:Buffers; D058428:Silica Gel; D012822:Silicon Dioxide; D011433:Propranolol; C010686:benzeneboronic acid