title: [Method for evaluating pulmonary clearance in mice and its pharmacological applications].
abstract: The purpose of this study was to develop a method for evaluating pulmonary clearance in mice that inhaled aerosolized 99mTc-labeled human serum albumin (33mTc-HSA). Mice were ventilated with an aerosol of 99mTc-HSA for 10 min with an ultrasonic nebulizer in an acrylic chamber. Pulmonary clearance was estimated from lung retention ratio at various times after inhalation of 99mTc-HSA. Lung retention ratios in mice were 72.4 +/- 3.3% at 1 hr, 60.1 +/- 2.3% at 3 hr and 34.8 +/- 2.0% at 24 hr after inhalation of 99mTc-HSA, similar to the previous results obtained in humans by cinescintigraphy. The data were analyzed on the basis of two-compartment model. Mabuterol, a beta 2-adrenoceptor agonist, stimulated the pulmonary clearance. In contrast, metyrapone, an adrenal 11-beta steroid hydroxylase inhibitor, suppressed the clearance with a decreased corticosterone content in plasma. These findings suggest that the developed method is useful for evaluating the pharmacological effects on pulmonary clearance.
authors.affiliation: Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kai
authors.forename: A
authors.initials: A
authors.lastname: Ishibashi
authors.forename: K
authors.initials: K
authors.lastname: Takahama
authors.forename: K
authors.initials: K
authors.lastname: Sakata
authors.forename: Y
authors.initials: Y
authors.lastname: Isohama
authors.forename: M
authors.initials: M
authors.lastname: Nakayama
authors.forename: A
authors.initials: A
authors.lastname: Sugii
authors.forename: T
authors.initials: T
authors.lastname: Miyata
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002976:Clenbuterol / Q000031:analogs & derivatives / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008722:Methods; D008797:Metyrapone / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D013668:Technetium Tc 99m Aggregated Albumin
chemical_list: D000318:Adrenergic beta-Agonists; D013668:Technetium Tc 99m Aggregated Albumin; C044573:mabuterol; D002976:Clenbuterol; D008797:Metyrapone