Bioactive glass promoted formation of nodules in periodontal-ligament fibroblasts in vitro.
The effects of bioactive glass and vitamin D3 on cultured fibroblasts derived from periodontal-ligament, with respect to their proliferation and alkaline-phosphatase activity were studied. The cells were cultured with or without the bioactive glass and/or vitamin D3, then the number and alkaline-phosphatase activity of the cells were measured periodically until the 33rd day. Formation of mineralized deposits was assessed by staining with alizarin red and von Kossa staining techniques. Fewer fibroblasts grew when they were cultured in the presence of bioactive glass and/or vitamin D3 as compared to those cultures without them. Alkaline-phosphatase activity was greater in the fibroblasts cultured with bioactive glass and vitamin D3 than in the cells grown without them. Mineralized deposits assessed by alizarin red and von Kossa staining techniques were observed microscopically around the fibroblasts cultured with bioactive glass and/or vitamin D3. A nodule visible after drying was evident only when both bioactive glass and vitamin D3 were present in culture. The results showed that although the bioactive glass and vitamin D3 decreased cell proliferation, they increased the alkaline-phosphatase activity of the fibroblasts which formed a nodule, suggesting an effect which might be useful for implant materials.
Kubo
K
K
Department of Periodontology, Kagoshima University Dental School, Japan.
Kakimoto
T
T
Kanda
C
C
Tsukasa
N
N
Uehara
M
M
Izumi
Y
Y
Kamada
T
T
Kaneko
N
N
Sueda
T
T
D000469:Alkaline Phosphatase / Q000187:drug effects; D001672:Biocompatible Materials / Q000378:metabolism / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002762:Cholecalciferol; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D005898:Glass / Q000032:analysis; D006801:Humans; D008903:Minerals / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
D001672:Biocompatible Materials; D008903:Minerals; D002762:Cholecalciferol; D000469:Alkaline Phosphatase