title: In vivo study of a high-porosity polytetrafluoroethylene graft: endothelialization, fluid leakage, and the effect of fibrin glue sealing.
abstract: Standard polytetrafluoroethylene (PTFE) grafts (30-microns internodal distance, ID) (ST grafts), high-porosity PTFE grafts (90 microns ID) (HP grafts), high-porosity PTFE grafts preclotted with autogenous blood (BHP grafts), and high-porosity PTFE grafts presealed with fibrin glue (FHP grafts) were implanted in both common carotid and femoral arteries of 18 dogs. Of the three high-porosity groups, the FHP graft showed the shortest bleeding time. Seromas and/or hematomas occurred as follows: ST grafts 1, HP grafts 7 (P < .05 vs ST), BHP grafts 5 and 2 with FHP grafts. Fibrin glue was observed in all histological sections of 1-week samples, but by 4 weeks it was almost totally absorbed. No endothelialization (ET) was measurable at 2 weeks. By 4 weeks ET extended for a short distance from each anastomosis and there were no significant differences between the four graft groups. At 18 weeks, the HP, BHP, and FHP grafts showed a significant increase in ET compared with the ST graft (P < .01) but there were no significant differences between the three types of high-porosity graft. The differences in patency rates and neointimal thicknesses did not reach statistical significance. High-porosity PTFE grafts showed superior endothelialization in dogs; however, the enlarged ID of PTFE grafts increased intraoperative bleeding and postoperative seroma formation. Fibrin glue sealant controlled bleeding through the graft wall without affecting graft healing, but its sealant effect was not enough to prevent late fluid leakage.
authors.affiliation: Department of Surgery, Harbor-UCLA Medical Center, Torrance 90509.
authors.forename: H
authors.initials: H
authors.lastname: Tsuchida
authors.forename: A
authors.initials: A
authors.lastname: Kashyap
authors.forename: B L
authors.initials: BL
authors.lastname: Cameron
authors.forename: S K
authors.initials: SK
authors.lastname: Peng
authors.forename: S E
authors.initials: SE
authors.lastname: Wilson
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D004285:Dogs; D004730:Endothelium, Vascular / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D015718:Fibrin Tissue Adhesive; D011138:Polytetrafluoroethylene
chemical_list: D015718:Fibrin Tissue Adhesive; D011138:Polytetrafluoroethylene