A regular 1:1 complex of two allosteric states in the single crystal of L-lactate dehydrogenase from Bifidobacterium longum.
Single crystals of the regular 1:1 complex of T and R-state tetramers of L-lactate dehydrogenase from Bifidobacterium longum have been grown from polyethylene glycol 6000 solution in the presence of NADH, fructose 1,6-bisphosphate and oxamate. The crystals belong to space group F222 with unit cell dimensions of a = 148.4 A, b = 295.9 A and c = 71.0 A. The crystals are suitable for X-ray analysis and diffract to beyond 2.5 A spacing. A crystallographic study showed that the asymmetric unit contains two subunits and that one of them belongs to the T-state tetramer and the other to the R-state tetramer.
Iwata
S
S
Department of Agricultural Chemistry, University of Tokyo, Japan.
Yoshida
S
S
Ohta
T
T
D000495:Allosteric Site; D001644:Bifidobacterium / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification; D046911:Macromolecular Substances; D008961:Models, Structural; D011487:Protein Conformation
D046911:Macromolecular Substances; D007770:L-Lactate Dehydrogenase