Cisplatin blocks mechano-electric transducer current in chick cochlear hair cells.
The effects of cisplatin (cis-dichlorodiammine platinum II, CDDP) on the mechano-electrical transduction (MET) current were investigated with a whole-cell patch-electrode voltage clamp technique in dissociated cochlear hair cells of chicks. CDDP blocked the MET channel in a dose- and voltage-dependent manner. At -50 mV, CDDP blocked the MET channel with a Hill coefficient of approximately 2 and a dissociation constant (KD) of 1.5 x 10(-3) M. The kinetics of CDDP blockade consist of a voltage-independent and a voltage-dependent component.
Kimitsuki
T
T
Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Nakagawa
T
T
Hisashi
K
K
Komune
S
S
Komiyama
S
S
D000818:Animals; D002645:Chickens; D002945:Cisplatin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D004594:Electrophysiology; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000502:physiology; D007700:Kinetics
D002945:Cisplatin