title: Technical aspects of buffy coat removal from whole blood and those of platelet production from single buffy coat units.
abstract: Technical aspects of buffy coat removal and those of platelet production from the buffy coat were studied using the so-called Amsterdam technique. A long stomach clamp physically separates the buffy coat from erythrocyte concentrate. A better removal of platelets and leukocytes was obtained when blood stored overnight was processed vs. fresh blood. As 71.4 +/- 16.4% of leukocytes was removed from blood stored overnight, it resulted in a mean leukocyte content of 0.84 +/- 0.52 x 10(-9) of the erythrocyte concentrate. The haematocrit of the single buffy coat units have an essential impact on platelet production from buffy coat. A haematocrit of less than 30% seems to be preferable with a volume over 100 ml. The leukocyte contamination of the platelet concentrate is less when it is produced from blood stored overnight.
authors.affiliation: National Institute of Haematology, Blood Transfusion and Immunology, Budapest.
authors.forename: Z
authors.initials: Z
authors.lastname: Rácz
authors.forename: C
authors.initials: C
authors.lastname: Baróti
mesh_terms: D001781:Blood Component Removal / Q000295:instrumentation*; D001787:Blood Group Incompatibility / Q000097:blood / Q000517:prevention & control; D001793:Blood Preservation; D004906:Erythrocyte Count; D017707:Erythrocyte Transfusion / Q000295:instrumentation*; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation*; D007958:Leukocyte Count; D010976:Platelet Count; D017713:Platelet Transfusion / Q000295:instrumentation; D010983:Plateletpheresis / Q000295:instrumentation*