Modulation of biological functions of Naegleria fowleri amoebae by growth medium.
Two strains of Naegleria fowleri amoebae were studied when the amoebae were maintained in the same growth medium or in two different media. A weakly pathogenic strain of N. fowleri, LEE, and a highly pathogenic strain, LEEmpC1, were compared for growth properties, the presence or absence of surface structures termed food cups, cytopathogenicity, cellular locomotion, susceptibility to complement-mediated lysis and immunological relatedness by western immunoblot analysis when grown in Nelson medium or in Cline medium. The two different strains of N. fowleri, LEE and LEEmpC1, were more similar in protein profiles and functional activity when both strains were grown in the same nutritional medium. Differences in growth, proteins synthesized, cytopathogenicity, susceptibility to complement lysis and rate of locomotion were noted when the same strain was grown in different media. Naegleria fowleri grown in Cline medium demonstrated an increased rate of growth, an increase in its rate of locomotion, an increased resistance to complement lysis, and destroyed target nerve cells by contact-dependent lysis. In contrast, the same strain of amoeba grown in Nelson medium showed slower growth, destroyed target cells by trogocytosis, and was less resistant to complement-mediated lysis.
Marciano-Cabral
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Department of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Toney
D M
DM
D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D003165:Complement System Proteins; D003470:Culture Media / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D016848:Naegleria fowleri / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000469:parasitology; D015800:Protozoan Proteins / Q000032:analysis; D013045:Species Specificity
D003470:Culture Media; D015800:Protozoan Proteins; D003165:Complement System Proteins