Effects of CGRP in different models of mouse ear inflammation.
The anti-inflammatory activity of calcitonin gene-related peptide (CGRP) has been studied in cutaneous inflammation induced by croton oil (CO), arachidonic acid (AA), tetradecanoylphorbol acetate (TPA) or cantharidin (CA). Our results show that mouse ear inflammation induced by CO, AA or TPA is decreased by topical administration of CGRP, whereas that induced by CA is not affected. The dose-response and temporal analysis of CGRP effect show that the maximal activity is present at the dose of 30 pmol/ear and when administered 30 min after the irritating agent. Moreover, pretreatment with capsaicin is able to mimic the anti-inflammatory effect of exogenous CGRP, while simultaneous administration of CGRP and capsaicin produces a reduced response. Our results suggest that CGRP released from sensory.
Clementi
G
G
Institute of Pharmacology, University of Catania School of Medicine, Italy.
Amico-Roxas
M
M
Caruso
A
A
Catena Cutuli
V M
VM
Prato
A
A
Maugeri
S
S
de Bernardis
E
E
Scapagnini
U
U
D000704:Analysis of Variance; D000818:Animals; D016718:Arachidonic Acid / Q000633:toxicity; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology / Q000627:therapeutic use*; D002193:Cantharidin / Q000633:toxicity; D002211:Capsaicin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D003436:Croton Oil / Q000633:toxicity; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004423:Ear; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D051379:Mice; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D016718:Arachidonic Acid; D003436:Croton Oil; D002193:Cantharidin; D015740:Calcitonin Gene-Related Peptide; D013755:Tetradecanoylphorbol Acetate; D002211:Capsaicin