title: Histological changes of implanted collagen material during bone induction.
abstract: Studies were made on the fate of implanted material during bone induction. Mixtures of 1 mg of crude bone morphogenetic protein (BMP), or bovine serum albumin as a control, and 1.5 mg of bovine collagen, were pressed into discs and implanted under the fascia of the rectus abdominus muscle of rats. The tissues with implants were fixed 7, 10, and 14 days later and examined histologically. On day 7 after implantation, the implant was surrounded and invaded by alkaline phosphatase-positive cells. New bone and cartilage were seen at the periphery of the implant. In the regions of calcified cartilage and bone, these osteogenic matrices were intermixed with the implant. The mineral deposits were seen by electron microscopy not only on the osteogenic matrices but also on the implanted collagen. On day 14, the bone had spread to the center of the implant. No osteogenesis or chondrogenesis was seen in control implants. It was concluded that the calcification occurred on the implanted collagen during bone induction, and that it was related to successive bone formation and remodeling.
authors.affiliation: Department of Removable Prosthodontics, School of Dentistry, Faculty of Pharmaceutical Sciences, Tokushima University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Horisaka
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: N
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: A
authors.initials: A
authors.lastname: Hirano
authors.forename: M
authors.initials: M
authors.lastname: Nishida
authors.forename: J
authors.initials: J
authors.lastname: Kawada
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Takagi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D019485:Bone Morphogenetic Proteins; D002118:Calcium / Q000378:metabolism; D002356:Cartilage / Q000166:cytology / Q000648:ultrastructure; D002417:Cattle; D003094:Collagen / Q000494:pharmacology; D004337:Drug Carriers; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D016348:Osseointegration; D019736:Prostheses and Implants; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D011506:Proteins; D003094:Collagen; D000469:Alkaline Phosphatase; D002118:Calcium