Application of biotinylated and 32P probes for detection of P-fimbriae in urinary E. coli.
Escherichia coli is the common causative agent of urinary tract infections. Twenty-six strains of Escherichia coli were isolated from children with pyelonephritis, symptomatic urinary tract infections and asymptomatic bacteriuria. Biotinylated and 32P-DNA probes were prepared for detection of P-fimbriae in the isolates. Of the 13 strains isolated from patients with pyelonephritis 11 were positive for the presence of the P gene by both probes. Strains isolated from cases of symptomatic urinary tract infections revealed the presence of P gene only in three samples of the total of nine isolated. None of the isolated E. coli strains from asymptomatic bacteriuria was found positive for the presence of the P gene. The biotinylated probe was simple and easily applicable in standard laboratory conditions and therefore the authors recommend it for use in diagnostic laboratories.
Jusková
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Institute of Preventive and Clinical Medicine, Bratislava, Slovakia.
Ciznár
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D000293:Adolescent; D001710:Biotin; D002240:Carbohydrate Sequence; D002648:Child; D002675:Child, Preschool; D015342:DNA Probes; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000652:urine; D005260:Female; D010861:Fimbriae, Bacterial / Q000737:chemistry*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010761:Phosphorus Radioisotopes; D014552:Urinary Tract Infections / Q000382:microbiology* / Q000652:urine
D015342:DNA Probes; D010761:Phosphorus Radioisotopes; D001710:Biotin