title: Detoxification of cyclophosphamide by human aldehyde dehydrogenase isozymes.
abstract: In in vitro studies, no turnover of aldophosphamide and mafosfamide was observed with the tumor-specific aldehyde dehydrogenase 3 isozyme (ALDH3) isolated from human stomach mucosa as well as from lung (A549) and pharynx (UMSCC2) carcinoma cell lines. Only the human liver cytosolic ALDH preparation (ALDH1) showed any significant oxidation of aldophosphamide and mafosfamide.
authors.affiliation: Institute of Human Genetics, University of Hamburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: von Eitzen
authors.forename: D
authors.initials: D
authors.lastname: Meier-Tackmann
authors.forename: D P
authors.initials: DP
authors.lastname: Agarwal
authors.forename: H W
authors.initials: HW
authors.lastname: Goedde
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology; D000444:Aldehyde Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D003520:Cyclophosphamide / Q000493:pharmacokinetics*; D005753:Gastric Mucosa / Q000201:enzymology; D006801:Humans; D008658:Inactivation, Metabolic; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008175:Lung Neoplasms / Q000201:enzymology; D010084:Oxidation-Reduction; D010610:Pharyngeal Neoplasms / Q000201:enzymology; D010752:Phosphoramide Mustards / Q000378:metabolism; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured
chemical_list: D007527:Isoenzymes; D010752:Phosphoramide Mustards; C006446:aldophosphamide; D003520:Cyclophosphamide; D000444:Aldehyde Dehydrogenase