A new tetracycline resistance determinant, Tet H, from Pasteurella multocida specifying active efflux of tetracycline.
The tetracycline resistance determinant on plasmid pVM111 from an avian strain of Pasteurella multocida mediates tetracycline resistance by a regulated active efflux mechanism. DNA coding for the determinant did not hybridize at high stringency with DNA representing a group of common tetracycline resistance determinants. The DNA sequence, however; revealed a structural gene and a repressor gene which had significant (37 to 64%) sequence similarities with previously described classes of efflux-type tetracycline resistance genes from members of the family Enterobacteriaceae. The new determinant has been assigned to class H.
Hansen
L M
LM
Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
McMurry
L M
LM
Levy
S B
SB
Hirsh
D C
DC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016979:Pasteurella multocida / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D013752:Tetracycline / Q000008:administration & dosage / Q000493:pharmacokinetics*; D013753:Tetracycline Resistance / Q000235:genetics* / Q000502:physiology; D014422:Turkeys
D004247:DNA; D013752:Tetracycline