Helicobacter pylori lipopolysaccharide inhibition of gastric mucosal laminin receptor: effect of sulglycotide.
1. The effect of cell-wall lipopolysaccharide from Helicobacter pylori, a bacterium implicated in the etiology of gastric disease, on the gastric mucosal laminin-receptor was investigated. 2. The receptor, isolated from gastric epithelial cell membrane by affinity chromatography on laminin-coupled Sepharose, was radioiodinated and incorporated into liposomes which exhibited specific affinity towards laminin-coated surface. 3. The binding of liposomal receptor to laminin-coated surface was inhibited by H. pylori lipopolysaccharide, which at 50 micrograms/ml caused a nearly complete (97%) inhibition in binding. 4. The inhibitory effect of the lipopolysaccharide was prevented by a cytoprotective agent, sulglycotide, that evoked a 92% restoration in binding at 40 micrograms/ml. 5. The results demonstrate that through its lipopolysaccharide H. pylori is capable of disrupting the gastric mucosal integrity and that this detrimental effect could be successfully countered by sulglycotide.
Piotrowski
J
J
Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Czajkowski
A
A
Yotsumoto
F
F
Slomiany
A
A
Slomiany
B L
BL
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D016480:Helicobacter pylori / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008081:Liposomes / Q000737:chemistry; D051381:Rats; D017474:Receptors, Laminin / Q000037:antagonists & inhibitors*; D012795:Sialoglycoproteins / Q000494:pharmacology*
D000897:Anti-Ulcer Agents; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; D008081:Liposomes; D017474:Receptors, Laminin; D012795:Sialoglycoproteins; C009354:sulglicotide