Nucleotide sequence of carnation ringspot dianthovirus RNA-1.
The nucleotide sequence of carnation ringspot virus (CRSV) RNA-1, the type member of the dianthovirus genus, has been determined. The 3756 nucleotide genomic RNA-1 contains three large open reading frames (ORFs), capable of encoding 27K, 54K and 38K polypeptides. In addition, a small ORF encoding a 10K polypeptide at the 3' terminus of the RNA has been identified. The gene organization of CRSV RNA-1 is similar to those of red clover necrotic mosaic (RCNMV) and sweet clover necrotic mosaic (SCNMV) dianthoviruses with the exception that CRSV RNA-1 contains the additional 3'-terminal ORF. The 27K and 54K proteins possess significant sequence similarity to corresponding polypeptides of the other dianthoviruses. The 54K protein also contains the conserved RNA-dependent RNA polymerase motif. The identification of a shifty heptanucleotide preceding the p27 ORF termination codon and a predicted secondary structure following the terminator suggest that a translational frameshifting event allows translation to continue past the p27 ORF into the p54 ORF, which is in the -1 frame, generating an 88K fusion protein. Amino acid sequence alignment of the 38K protein with the corresponding RCNMV and SCNMV polypeptides indicate that this is the viral capsid protein.
Ryabov
E V
EV
Institute of Agricultural Biotechnology, Moscow, Russia.
Generozov
E V
EV
Kendall
T L
TL
Lommel
S A
SA
Zavriev
S K
SK
D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010942:Plant Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
D012367:RNA, Viral