In vivo regulation of the assembly and intracellular transport of class I major histocompatibility complex molecules.
Using H-2Kb-transfected Balb/c 3T3 cells which generate "empty" H-2Kb molecules devoid of antigenic peptides, we show that peptide availability determines the stability of class I molecules and dictates the overall intracellular transport rate of the class I complexes. Our data also indicate that chaperonin-like proteins are involved in class I assembly. Using Drosophila cells transfected with H-2Kb and murine beta 2-microglobulin, we show that one possible candidate, calnexin, associates with class I molecules prior to peptide acquisition. These data suggest that both peptide supply and assembly proteins dictate cell surface expression of class I major histocompatibility complex molecules and ultimately influence T cell recognition. The role of beta 2-microglobulin in class I assembly is also discussed.
Song
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ES
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
Yang
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Y
Jackson
M R
MR
Peterson
P A
PA
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001692:Biological Transport; D002135:Calcium-Binding Proteins / Q000378:metabolism; D037281:Calnexin; D002460:Cell Line; D002461:Cell Line, Transformed; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D004330:Drosophila; D004591:Electrophoresis, Polyacrylamide Gel; D006183:H-2 Antigens / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008223:Lymphoma; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011499:Protein Processing, Post-Translational; D014162:Transfection; D001613:beta 2-Microglobulin / Q000096:biosynthesis / Q000378:metabolism
D000906:Antibodies; D002135:Calcium-Binding Proteins; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D008565:Membrane Proteins; D010455:Peptides; D001613:beta 2-Microglobulin; D037281:Calnexin