[The genetic application for Japanese patients with coronary artery diseases using restriction fragment length polymorphisms (RFLPs) of apolipoprotein gene].
We applied restriction fragment length polymorphisms (RFLPs) for apolipoprotein (apoAI, apoCIII, apoAIV, apoCI, and apoB) genes to analyze differences between Japanese and Caucasian in allele frequencies as well as those between normal Japanese subjects and patients with coronary artery disease confirmed with coronary arteriography. Although the allelic fragment sizes in the two racial groups showed complete correspondence, the allelic frequencies of these markers detected by the combination of probes and restriction enzymes showed 60% difference in Japanese and Caucasian. There was a significant (p < 0.01) difference between the normal subjects (with arteriographically-confirmed absence of stenosis in all coronary branches) and those with severe coronary artery disease (50 to 100% stenosis in at least one branch) in plasma levels of apoAI, apoB, total cholesterol, HDL-cholesterol, and LDL-cholesterol. RFLP analysis for 13 markers, however, revealed no significant difference between these groups when analyzed in subjects of all ages (range, 12 to 82 years; N = 306). However, when analysis was restricted to data for 12-to 50-year-olds, a significant (p < 0.02) difference between the normal control group and the severe coronary artery disease group in 3' apoB probe and EcoRI enzyme test results. An acquired and environmental factors were assumed to be involved in this disease because the correlation between allelic frequencies and coronary artery disease status showed dilution when the subject age distribution included older subjects.
Arakawa
M
M
Genetic Research Laboratory, SRL, Hachioji.
Hirakawa
S
S
Itoh
H
H
Mori
N
N
Yasue
T
T
Watanabe
S
S
Tanaka
T
T
Uemura
H
H
Yamaguchi
M
M
Takaya
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001053:Apolipoproteins / Q000097:blood / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D015139:Blotting, Southern; D002648:Child; D003327:Coronary Disease / Q000208:ethnology* / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D044465:White People / Q000235:genetics
D001053:Apolipoproteins