[Determination of restriction fragment length polymorphism (RFLP) using digital image analysis].
The use of a video system to analyse restriction fragment length polymorphisms (RFLP) was assessed concerning precision and accuracy. In comparison to a manual, ruler based measurement technique the automated system (Bioimage/Millipore) showed excellent agreement in optical resolution and precision, having a little smaller differences between multiple measurements of restriction fragments. The variation between kb-values detected with both procedures is very small and lies in the dimension of the optical resolution of both measurement techniques. Moreover the fragment sizes are scattered rather homogeneously around a fictive mean value, at which the video system in part shows slightly higher kb-numbers.
Bässler
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Kriminaltechnischen Institut des Landeskriminalamtes Baden-Württemberg.
D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D005787:Gene Frequency; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D012150:Polymorphism, Restriction Fragment Length; D014741:Video Recording / Q000295:instrumentation*
D015342:DNA Probes; D004247:DNA
Bestimmung von Restriktionsfragment-Längenpolymorphismen (RFLP) mit Hilfe der digitalen Bildanalyse.