Human erythrocyte pyrimidine 5'-nucleotidase isoenzymes: effect of sulfhydryl reagents and electrophoretic discrimination.
The electrophoretic behavior of human red cell pyrimidine 5'-nucleotidase isozymes (UMPH1 and UMPH2) was studied on starch gels with and without treatment with thiol reagents. It was found that at least one reactive sulfhydryl group occurs in the UMPH1 isozyme but not in the UMPH2 isozyme. An electrophoretic system is described that allows the discrimination of UMPH1 and UMPH2 isozymes.
Manco
L
L
Departamento de Antropologia, Universidade de Coimbra, Portugal.
Amorim
A
A
D015720:5'-Nucleotidase / Q000097:blood*; D004586:Electrophoresis / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D013439:Sulfhydryl Reagents
D007527:Isoenzymes; D013439:Sulfhydryl Reagents; D015720:5'-Nucleotidase