Interleukin-6 and interleukin-8 production by mononuclear cells of chronic alcoholics during treatment.
Chronic alcohol consumption has been associated with suppression of a number of immune parameters. This study was designed to investigate the relationship between chronic alcohol ingestion and cessation with respect to release of interleukin-6 (IL-6) and interleukin-8 (IL-8) using highly specific and sensitive ELISA assays, as well as a functional assay, natural killer cell cytotoxic activity. ELISAs were developed to determine the amount of IL-6 and IL-8 release by peripheral blood mononuclear cells (PBMCs). Two groups of subjects were recruited: young (18-22 years old), nonalcoholic users (controls) and long-term alcoholics (35-55 years old). Blood samples were collected at time 0 from all subjects and from alcoholics 28 days after treatment had begun and alcohol use had ceased. Then mitogen-stimulated release of cytokines by peripheral blood cells was determined. The abstaining controls, and the alcoholics, after 30 days of abstinence, tended to produce lower amounts of IL-6 and IL-8, although these differences were not statistically significant. Natural killer cell activity was not statistically different between the young groups, yet appeared to increase once alcohol use discontinued. Some of the cells from the controls (abstainers) were incubated with ethanol (EtOH). Its content in sealed wells was measured after the time of incubation of PBMCs. When EtOH was serially diluted in plates, some well-well diffusion was noted, but the maximum concentration of EtOH never fell below 0.3% from an initial concentration of 0.5%, and at no time was the EtOH concentration gradient completely lost, even after 66 hr of incubation.(ABSTRACT TRUNCATED AT 250 WORDS)
Martinez
F
F
Department of Family and Community Medicine, NIAAA Alcohol Research Center, University of Arizona, Tucson 85724.
Thomas
N M
NM
Darban
H
H
Cox
T J
TJ
Wood
S
S
Watson
R R
RR
D000293:Adolescent; D000328:Adult; D000428:Alcohol Drinking / Q000009:adverse effects / Q000276:immunology; D000430:Alcohol Withdrawal Delirium / Q000276:immunology* / Q000534:rehabilitation; D000437:Alcoholism / Q000276:immunology* / Q000534:rehabilitation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008108:Liver Diseases, Alcoholic / Q000276:immunology / Q000534:rehabilitation; D008297:Male; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology
D015850:Interleukin-6; D016209:Interleukin-8