Construction of a yeast artificial chromosome contig spanning the pseudoautosomal region and isolation of 25 new sequence-tagged sites.
Thirty-one yeast artificial chromosomes (YACs) from the human pseudoautosomal region were identified by a combination of sequence-tagged site (STS) screenings and colony hybridizations, using a subtelomeric interspersed repetitive element mapping predominantly to the pseudoautosomal region. Twenty-five new pseudoautosomal STSs were generated, of which 4 detected restriction fragment length polymorphisms. A total of 33 STSs were used to assemble the 31 YACs into a single contiguous set of overlapping DNA fragments spanning at least 2.3 megabases of the pseudoautosomal region. In addition, four pseudoautosomal genes including hydroxyindole O-methyltransferase have been positioned on this set of fragments.
Slim
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R
Unité de Génétique Moléculaire Humaine (CNRS URA 1445), Insitut Pasteur, Paris, France.
Le Paslier
D
D
Compain
S
S
Levilliers
J
J
Ougen
P
P
Billault
A
A
Donohue
S J
SJ
Klein
D C
DC
Mintz
L
L
Bernheim
A
A
D000121:Acetylserotonin O-Methyltransferase / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D016324:Sequence Tagged Sites; D014960:X Chromosome; D014998:Y Chromosome
D004247:DNA; D000121:Acetylserotonin O-Methyltransferase