Rapid, amplification-based fingerprinting of Mycobacterium tuberculosis.
Insertion element IS6110 occurs in multiple copies throughout the Mycobacterium tuberculosis genome, and the variability of its insertion sites is the basis for the IS6110 restriction fragment length polymorphism (RFLP) method for typing. We describe a novel gene amplification method to assess the variability of the location of IS6110. A unilateral-nested polymerase chain reaction and hybridization procedure was used to measure the variability in the distances between IS6110 elements and copies of a major polymorphic tandem repeat sequence of M. tuberculosis. The pattern of amplicons produced could be used to cluster epidemiologically related strains of M. tuberculosis into groups which correlated with the groups formed using IS6110-RFLP typing. Reliable patterns can be generated directly from sputum specimens as well as from M. tuberculosis cultures. We designated the novel method as IS6110-ampliprinting.
Plikaytis
B B
BB
Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333.
Crawford
J T
JT
Woodley
C L
CL
Butler
W R
WR
Eisenach
K D
KD
Cave
M D
MD
Shinnick
T M
TM
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002874:Chromosome Mapping; D016172:DNA Fingerprinting / Q000379:methods*; D004251:DNA Transposable Elements / Q000235:genetics*; D004196:Disease Outbreaks; D004352:Drug Resistance, Microbial / Q000235:genetics; D005784:Gene Amplification; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D009841:Oligonucleotides; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D013183:Sputum / Q000382:microbiology; D014376:Tuberculosis / Q000382:microbiology
D004251:DNA Transposable Elements; D009841:Oligonucleotides