title: Splanchnic and systemic hemodynamic responses to portal vein endotoxin after resuscitation from hemorrhagic shock.
abstract: BACKGROUND
  Hemorrhagic shock and sepsis are usually studied separately or in rodents. This study combined the two insults in a large animal model.
  
  
  METHODS
  Anesthetized pigs were bled, held in shock for 1 hour, and then resuscitated with fluid. After 3 days, Escherichia coli endotoxin LPS was infused into the portal vein (150 micrograms/kg x 30 min) to mimic the effect of enteric substances breaching the mucosal barrier. Systemic and splanchnic hemodynamics, circulating leukocytes, and plasma levels of tumor necrosis factor (TNF) were measured in five groups: 40% hemorrhage plus fluid only resuscitation, 40% hemorrhage plus fluid-blood resuscitation, 50% hemorrhage plus fluid-blood resuscitation, sham, or sham plus 5 micrograms/kg LPS priming dose instead of hemorrhage.
  
  
  RESULTS
  On day 4 before infusion of LPS, there were no differences between groups in hemodynamics or O2 utilization, but systemic O2 delivery and O2 consumption were each reduced in the hemorrhaged groups because of the hemodilution associated with resuscitation. For 3 hours after infusion of LPS, all animals received aggressive fluid and respiratory support, but arterial blood pressure decreased, and systemic O2 utilization, splanchnic O2 utilization, and arterial lactate level increased; there were no differences between groups. In the 50% group compared with sham, LPS-evoked decreases in cardiac index and stroke index were eliminated, and LPS-evoked tachycardia, pulmonary and systemic vasoconstriction, and increases in hepatic and portal vein lactate levels were blunted. Despite similar leukocyte counts before infusion of LPS and similar leukopenia after LPS infusion, plasma LPS level was higher in the 50% group compared with sham. Furthermore, LPS evoked increases in portal and hepatic vein plasma TNF in the shams, but those changes were reduced in the 50% group.
  
  
  CONCLUSIONS
  Most responses to LPS were similar after hemorrhagic shock or a sham operation, which is inconsistent with the concept of "priming." LPS-evoked increases in plasma TNF were blunted after shock, probably because of trauma-induced immune dysfunction. A combined shock plus septic challenge in a large animal model may be valuable for investigating the pathogenic mechanism in human beings.
authors.affiliation: Department of Physiology, University of Tennessee Health Science Center, Memphis 38163.
authors.forename: T J
authors.initials: TJ
authors.lastname: Gavin
authors.forename: T C
authors.initials: TC
authors.lastname: Fabian
authors.forename: J D
authors.initials: JD
authors.lastname: Wilson
authors.forename: L L
authors.initials: LL
authors.lastname: Trenthem
authors.forename: F E
authors.initials: FE
authors.lastname: Pritchard
authors.forename: M A
authors.initials: MA
authors.lastname: Croce
authors.forename: R M
authors.initials: RM
authors.lastname: Stewart
authors.forename: K G
authors.initials: KG
authors.lastname: Proctor
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001775:Blood Circulation; D001803:Blood Transfusion; D004195:Disease Models, Animal; D004731:Endotoxins / Q000097:blood*; D004926:Escherichia coli; D005260:Female; D005440:Fluid Therapy; D006439:Hemodynamics; D007108:Immune Tolerance / Q000502:physiology; D007970:Leukopenia / Q000209:etiology; D008070:Lipopolysaccharides / Q000097:blood; D008297:Male; D010101:Oxygen Consumption; D012771:Shock, Hemorrhagic / Q000097:blood / Q000150:complications* / Q000503:physiopathology; D012772:Shock, Septic / Q000097:blood / Q000150:complications* / Q000503:physiopathology; D013152:Splanchnic Circulation; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000276:immunology
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha