In vitro platelet interactions in whole human blood exposed to biomaterial surfaces: insights on blood compatibility.
A short-term in vitro test to study platelet interactions with biomaterials is described. Using fresh human blood and a modified Chandler loop system, beta-thromboglobulin release was measured. Also, adherent platelets were observed by using scanning electron microscopy (SEM) and a colorimetric stain specific for human platelet GPIIIa. Materials studied in these experiments were polyethylene (PE), Biomer, poly(vinyl alcohol) (PVA), and a polyurethane prepared with octadecyl pendant groups (ODCE). Four blood reactions were observed: (1) Platelets continually adhere and activate on the Biomer; (2) platelets initially adhere and activate but then spread to a thin, passivating film on the PE; (3) platelets do not adhere to the PVA surface but continually react with it upon contact; and (4) platelets neither adhere to nor activate on the ODCE surface. Reactions (2) and (4) are considered characteristic of blood-compatible materials.
Haycox
C L
CL
Center for Bioengineering, University of Washington, Seattle 98195.
Ratner
B D
BD
D001672:Biocompatible Materials / Q000009:adverse effects; D001775:Blood Circulation / Q000187:drug effects; D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000737:chemistry; D007519:Isoantigens / Q000097:blood; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010976:Platelet Count / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D013499:Surface Properties / Q000187:drug effects; D013927:Thrombosis / Q000139:chemically induced / Q000517:prevention & control*; D001620:beta-Thromboglobulin / Q000187:drug effects / Q000276:immunology
D001672:Biocompatible Materials; D007518:Isoantibodies; D007519:Isoantigens; D010980:Platelet Membrane Glycoproteins; D001620:beta-Thromboglobulin