[An evaluation of the Khramkova-Abelev immunodiffusion method for determining the lectin concentration in a solution].
Using the method of double radial immunodiffusion with highly purified preparations of HRPO and lectins PSA, PNA, SNA the threshold sensitivity of precipitation in agar gel, 7.2 micrograms/ml, was established. The use of monospecific immunodiffusion test system for PNA helped increase the sensitivity of immunodiffusion analysis to 3.6 micrograms/ml.
Kalinin
Iu A
IuA
Tatarinov
Iu S
IuS
Terent'ev
A A
AA
D000818:Animals; D016009:Chi-Square Distribution; D005069:Evaluation Studies as Topic; D006801:Humans; D005779:Immunodiffusion / Q000379:methods* / Q000706:statistics & numerical data; D037102:Lectins / Q000032:analysis* / Q000302:isolation & purification; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification; D012996:Solutions
D037102:Lectins; D010940:Plant Proteins; D012996:Solutions
Otsenka immunodiffuzionnogo metods Khramkovoĭ--Abeleva dlia opredeleniia kontsentratsii lektinov v rastvore.