title: Evidence for at least three transcribed BoLA class I loci.
abstract: Serological data identify a single major histocompatibility complex (MHC) class I locus in cattle. Molecular data, however, demonstrate the presence of at least two cattle MHC (BoLA) class I loci. To investigate the number of transcribed BoLA class I genes, we amplified cattle cDNA by using a single MHC class I-specific primer that hybridized to a conserved region of exon 4 and a non-specific 3' primer. Six BoLA class I cDNAs have been cloned and sequenced from a Bos taurus bull heterozygous for BoLA class I serological antigens, demonstrating the presence of a minimum of three loci. Sequence comparisons suggested that one of these cDNAs may be an unexpressed allele or the product of a nonclassical locus.
authors.affiliation: Texas A & M University, Department of Veterinary Pathology, College Station 77843.
authors.forename: T L
authors.initials: TL
authors.lastname: Garber
authors.forename: A L
authors.initials: AL
authors.lastname: Hughes
authors.forename: D I
authors.initials: DI
authors.lastname: Watkins
authors.forename: J W
authors.initials: JW
authors.lastname: Templeton
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D002417:Cattle / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005805:Genes, MHC Class I; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary