Biochemical and immunological characterization of the acrosome reaction-inducing substance (ARIS) of hFF.
Gel filtration experiments with 3H-progesterone labeled hFF demonstrated that the AR-inducing activity of hFF might be mediated by a progesterone-binding protein. Further immunological investigations added evidence in support to ARIS being identical with the SERPIN CBG. Moreover, only the protein-progesterone-complex was able to induce AR. It is suggested that the CBG-progesterone-complex is proteolytically cleaved at the plasma membrane of spermatozoa, releasing a high local concentration of progesterone which leads to induction of the AR.
Miska
W
W
Department of Dermatology and Andrology, Justus Leibig University, Giessen, Germany.
Fehl
P
P
Henkel
R
R
D000177:Acrosome / Q000502:physiology*; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008970:Molecular Weight; D011374:Progesterone / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry* / Q000276:immunology*; D014156:Transcortin / Q000502:physiology
D011506:Proteins; D011374:Progesterone; D014156:Transcortin