The electrophoretic karyotype of two strains of Candida albicans by transverse alternate field electrophoresis reveals higher number of chromosomes ranging from 1 to 3.5 Mb.
The advent of the powerful electrophoretic technique, pulsed field gel electrophoresis, first developed on the yeast Saccharomyces cerevisiae, has brought a vital impulse to the genetic study on the opportunistic pathogen Candida albicans. We report here on sizing and numbering of Candida chromosomes using transverse alternate field electrophoresis. Our results indicate the occurrence of nine to ten electrophoretic bands (depending on type of Candida strain), that range in approximate size from 1 to 3.5 Mbp, and may account for a higher overall chromosome number, because at least two of these bands appear to be doublets. This number of bands, with smaller size, is considerably higher than previously reported.
Allegrucci
M
M
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Lanfaloni
L
L
Bietta
C
C
Spaccapelo
R
R
Fioretti
M C
MC
Bistoni
F
F
D002176:Candida albicans / Q000235:genetics*; D015825:Chromosomes, Fungal; D004586:Electrophoresis / Q000379:methods; D016521:Electrophoresis, Gel, Pulsed-Field; D007621:Karyotyping / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics; D013045:Species Specificity