N-ethylmaleimide inhibition of silver-enhanced sodium transport across toad (Bufo macinus) skin.
1. Silver stimulated short-circuit current and transepithelial potential difference. 2. N-Ethylmaleimide inhibited the silver-induced short-circuit current. 3. There was a biphasic inhibition of silver-induced short-circuit current by N-ethylmaleimide. 4. There is a specific, maximal number of sulfhydryl groups associated with active sodium absorption. 5. Stimulation of active sodium transport by antidiuretic hormone was blunted by the presence of silver. 6. The silver-induced short-circuit current is carried by a net active sodium transfer from the outside to the inside bathing solution.
Gerencser
G A
GA
Department of Physiology, College of Medicine, University of Florida, Gainesville 32610.
Loo
S Y
SY
Cornette
K M
KM
D000818:Animals; D001692:Biological Transport; D002025:Bufonidae; D005033:Ethylmaleimide / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D012834:Silver / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels / Q000378:metabolism*
D015222:Sodium Channels; D012834:Silver; D012964:Sodium; D005033:Ethylmaleimide