title: Specific expression of insulin-like growth factor-II in rhabdomyosarcoma tumor cells.
abstract: Insulin-like growth factor II (IGF-II) acts as autocrine growth and motility factor in human rhabdomyosarcoma cell lines, and Northern blot analysis of tumor biopsy specimens from both alveolar and embryonal rhabdomyosarcoma demonstrates high levels of IGF-II mRNA expression. To determine the frequency and site of expression of IGF-II in these tumors, the authors performed in situ hybridization. All tumor specimens examined expressed the gene for IGF-II, and this expression was localized to the tumor cells themselves and not to the surrounding stroma. These data suggest that the IGF-II autocrine loop may be operating not only in vitro but also in vivo.
authors.affiliation: Pediatric Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: C P
authors.initials: CP
authors.lastname: Minniti
authors.forename: M
authors.initials: M
authors.lastname: Tsokos
authors.forename: W A
authors.initials: WA
authors.lastname: Newton
authors.forename: L J
authors.initials: LJ
authors.lastname: Helman
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D015152:Blotting, Northern; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D007223:Infant; D007335:Insulin-Like Growth Factor II / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D012208:Rhabdomyosarcoma / Q000737:chemistry* / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm; D007335:Insulin-Like Growth Factor II