title: Analysis of L-dopa in pharmaceutical preparations and of total phenols content in urine by means of an enzyme-amperometric sensor.
abstract: An enzyme-amperometric method is proposed for the analysis of total phenols and L-dopa; the method is based on the enzyme tyrosinase, which is immobilized in a Nylon membrane and coupled to an oxygen gas-diffusion amperometric electrode. The method was applied to the determination of total phenols in urine and to L-dopa in formulations and was evaluated as a promising alternative to currently adopted methods, e.g. to a classical spectrophotometric technique, chosen as a reference method.
authors.affiliation: Chemistry Department, University La Sapienza, Roma, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Campanella
authors.forename: T
authors.initials: T
authors.lastname: Beone
authors.forename: M P
authors.initials: MP
authors.lastname: Sammartino
authors.forename: M
authors.initials: M
authors.lastname: Tomassetti
mesh_terms: D015374:Biosensing Techniques; D004800:Enzymes, Immobilized; D006801:Humans; D007980:Levodopa / Q000032:analysis*; D008567:Membranes, Artificial; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry; D010636:Phenols / Q000652:urine*
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009757:Nylons; D010636:Phenols; D007980:Levodopa; D014442:Monophenol Monooxygenase