Screening of plant constituents for effect on glucose transport activity in Ehrlich ascites tumour cells.
The effect of plant extracts on D-glucose uptake by Ehrlich ascites tumour cells was examined. Among the 23 extracts of medicinal plants, five samples inhibited, and six samples activated, the uptake significantly. From one of the active plants, Lagerstroemia speciosa, two triterpenoids, colosolic acid and maslinic acid were isolated. Colosolic acid was shown to be a glucose transport activator. Since this compound was known to have hypoglycemic activity, our simple in vitro bioassay method can at least be used as a first screening for anti-diabetic activity.
Murakami
C
C
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
Myoga
K
K
Kasai
R
R
Ohtani
K
K
Kurokawa
T
T
Ishibashi
S
S
Dayrit
F
F
Padolina
W G
WG
Yamasaki
K
K
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D010936:Plant Extracts; D003847:Deoxyglucose; D005947:Glucose