Anticentromere autoantibodies. Evaluation of an ELISA using recombinant fusion protein CENP-B as antigen.
OBJECTIVE
To evaluate an enzyme-linked immunosorbent assay (ELISA) for anticentromere autoantibodies (ACA).


METHODS
Sera from 611 patients with scleroderma, CREST (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasias), Raynaud's disease, and connective tissue disease control patients were studied by ELISA using the fusion protein CENP-B, by immunofluorescence on dividing HEp-2 cells, and by immunoblotting on chromosomes and CENP-B.


RESULTS
Compared with immunofluorescence, the CENP-B ELISA sensitivity was 94% and the specificity was 93%. In 19.7% of the cases, there was a probability of a false-positive result and in 1.9%, a probability of a false-negative result, yielding positive and negative predictive values of 0.80 and 0.98, respectively.


CONCLUSIONS
The CENP-B ELISA is a sensitive and specific assay for ACA.
Vázquez-Abad
D
D
University of Connecticut School of Medicine, Farmington.
Wallace
S
S
Senécal
J L
JL
Joyal
F
F
Roussin
A
A
Earnshaw
W C
WC
Rothfield
N
N
D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000276:immunology*; D001324:Autoantigens; D017675:CREST Syndrome / Q000276:immunology; D002503:Centromere / Q000276:immunology*; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D003240:Connective Tissue Diseases / Q000276:immunology; D004268:DNA-Binding Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D011928:Raynaud Disease / Q000276:immunology; D011994:Recombinant Proteins; D012594:Scleroderma, Localized / Q000276:immunology; D012680:Sensitivity and Specificity
D000906:Antibodies; D000941:Antigens; D001324:Autoantigens; C497926:CENPB protein, human; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins