A leukocyte integrin binding peptide from intercellular adhesion molecule-2 stimulates T cell adhesion and natural killer cell activity.
Adhesion is of pivotal importance for a number of leukocyte functions. Little is known about the binding between leukocyte integrins and the intercellular adhesion molecules (ICAMs). Normally integrins are nonadhesive, and require a stimulus to become active. We have now identified a peptide from ICAM-2, which binds to leukocyte integrins and activates adhesion. Furthermore, the peptide strongly increased the binding and cytotoxicity of natural killer cells. These findings show that adhesion-dependent leukocyte functions can be activated by ligand-derived peptides, and therefore provide evidence that the avidity of leukocyte integrins is up-regulated by integrin-ligand interactions.
Li
R
R
Department of Biochemistry, University of Helsinki, Finland.
Nortamo
P
P
Kantor
C
C
Kovanen
P
P
Timonen
T
T
Gahmberg
C G
CG
D000470:Alkaloids / Q000494:pharmacology; D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000378:metabolism; D018821:CD18 Antigens; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic; D004988:Ethers, Cyclic / Q000494:pharmacology; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D008969:Molecular Sequence Data; D019319:Okadaic Acid; D010446:Peptide Fragments / Q000378:metabolism*; D011485:Protein Binding; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D016028:Receptors, Leukocyte-Adhesion / Q000378:metabolism; D019311:Staurosporine; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D015703:Antigens, CD; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D004988:Ethers, Cyclic; C065289:ICAM2 protein, human; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D010446:Peptide Fragments; D016028:Receptors, Leukocyte-Adhesion; D019319:Okadaic Acid; D011493:Protein Kinase C; D019311:Staurosporine