Sequence analysis of a cDNA for lysyl hydroxylase isolated from human skin fibroblasts from a normal donor: differences from human placental lysyl hydroxylase cDNA.
Using polymerase chain reaction, we have isolated and sequenced a 3-kb cDNA for lysyl hydroxylase (LH) from human skin fibroblasts from an normal donor. Apart from two polymorphic sites, no differences were observed between the 2184 nt coding regions of LH cDNA from fibroblasts and placenta. However, four differences were observed in the 3' non-coding regions of the two cDNAs; three were single base changes and the fourth a deletion of a single base. The absence of the single nucleotide in the LH cDNA from fibroblasts resulted in the loss of an HpaII site that is present in the placental LH cDNA; this was confirmed in HpaII digests of fibroblast and placental LH cDNAs from the same donor. Northern blots showed that the LH gene was strongly expressed in fibroblasts and placenta and, to a lesser extent, in aorta, lung, vein, cartilage, and artery.
Yeowell
H N
HN
Duke University Medical Center, Division of Dermatology, Durham, North Carolina 27710.
Ha
V
V
Clark
W L
WL
Marshall
M K
MK
Pinnell
S R
SR
D001483:Base Sequence; D015152:Blotting, Northern; D015342:DNA Probes; D018076:DNA, Complementary / Q000737:chemistry*; D005347:Fibroblasts / Q000201:enzymology*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D012867:Skin / Q000166:cytology* / Q000201:enzymology
D015342:DNA Probes; D018076:DNA, Complementary; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase