How is leghemoglobin involved in peribacteroid membrane degradation during nodule senescence?
An increase in the rate of succinate and glutamate uptake by isolated symbiosomes from French bean nodules was observed in the presence of iron plus H2O2. The lipid bilayer, and not proteins involved in transport, seems to be the major target of radical attack. Leghemoglobin in the presence of a 6-fold excess of H2O2 (where heme breakdown and iron release occurred) provoked also an increase in peribacteroid membrane permeability. In contrast, this hemoprotein in the presence of a 2-fold excess of H2O2 (where a protein radical was generated) was without effect. We suggest that in vivo the release of heme iron may constitute the major process concerning the involvement of leghemoglobin in the degradation of the peribacteroid membrane during nodule senescence.
Herrada
G
G
Laboratoire de Biologie Végétale et Microbiologie, URA CNRS 1114 Université de Nice-Sophia Antipolis, Pare Valrose, France.
Puppo
A
A
Moreau
S
S
Day
D A
DA
Rigaud
J
J
D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D007887:Fabaceae / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure; D005609:Free Radicals; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D007874:Leghemoglobin / Q000494:pharmacology*; D008051:Lipid Bilayers / Q000378:metabolism; D010946:Plants, Medicinal; D012231:Rhizobium / Q000378:metabolism* / Q000648:ultrastructure; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D013559:Symbiosis
D005609:Free Radicals; D005971:Glutamates; D007874:Leghemoglobin; D008051:Lipid Bilayers; D013386:Succinates; D018698:Glutamic Acid; D019802:Succinic Acid; D006861:Hydrogen Peroxide; D007501:Iron