Ethanol promotes rapid depletion of intracellular free Mg in cerebral vascular smooth muscle cells: possible relation to alcohol-induced behavioral and stroke-like effects.
The acute effects of ethanol on intracellular free magnesium ions ([Mg2+]i) in cultured canine cerebral vascular smooth muscle cells (VSMCs) were studied by digital imaging microscopy using the Mg2+ fluorescent probe mag-fura-2. In 0 mM ethanol, the basal level of [Mg2+]i was between 500-700 microM with a heterogeneous distribution within the cells; [Mg2+]i was greater in the perinuclear than in the peripheral region. Treatment of the cells with 10, 25, and 100 mM ethanol resulted in rapid (within 30 s) concentration-dependent reduction in [Mg2+]i; the greater the concentration and the greater the duration of acute exposure, the greater the fall in [Mg2+]i. Exposure of cerebral VSMCs to 100 mM ethanol resulted in a 57% reduction in [Mg2+]i (i.e., from 510 +/- 40 to 220 +/- 30 microM). These observations are consistent with the tenet that "binge drinking" of ethanol could result in cerebrovasospasm, ischemia, and rupture of cerebral blood vessels as a consequence of depletion of cerebral VSMC [Mg2+]i. Deficits in [Mg2+]i, O2, and nutrient delivery could account in part for some of the behavioral actions of alcohol.
Altura
B M
BM
Department of Physiology, State University of New York, Brooklyn 11203.
Zhang
A
A
Cheng
T P
TP
Altura
B T
BT
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D002561:Cerebrovascular Disorders / Q000139:chemically induced; D004285:Dogs; D000431:Ethanol / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*
D000431:Ethanol; D008274:Magnesium