Differential effect of human and murine polyclonal and monoclonal antisera on TNF-alpha production by human monocytes.
The capacity of human and murine polyclonal and monoclonal antibodies to inhibit lipopolysaccharide (LPS)-induced tumor necrosis factor-alpha (TNF-alpha) release from human monocytes was investigated. Human pooled immunoglobulin G (IVIG), human IgM monoclonal antibody (HA-1A) directed against the lipid A moiety of LPS, and murine IgG monoclonal antibody (MT-1F) raised in mice against antibiotic-treated Escherichia coli O6:K- were either added simultaneously with LPS to monocytes or preincubated for 1 h at 37 degrees C before being added to monocytes. TNF-alpha content in the monocyte supernatants was then tested. Simultaneous addition of increasing concentrations of IVIG (from 0.3 to 2.5 mg/ml) and 10 micrograms/ml of LPS to monocytes induced an enhanced release of TNF-alpha by monocytes in a dose dependent fashion. Preincubation of IVIG with LPS abolished the additive effect, but did not inhibit LPS-induced TNF-alpha release by monocytes. The simultaneous addition of LPS and HA-1A to monocytes had no additive effect nor did it inhibit TNF-alpha release. On the other hand, inhibition of TNF-alpha release was observed when HA-1A was preincubated with LPS before being added to monocytes. In all instances MT-1F inhibited TNF-alpha release when the monocytes were stimulated with smooth type LPS, but not with LPS isolated from rough mutants.
Raponi
G
G
I Chair of Clinical Microbiology, Faculty of Medicine, La Sapienza University of Rome, Italy.
Lun
M T
MT
Gaeta
A
A
Ghezzi
M C
MC
Nazzari
C
C
Mancini
C
C
Filadoro
F
F
Bartolazzi
A
A
Natali
P
P
Rozenberg-Arska
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D016756:Immunoglobulins, Intravenous; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha