Analysis of FCE 23762 (methoxymorpholinodoxorubicin hydrochloride), a new antitumour agent, by HPTLC and scanning densitometry.
A simple, rapid and reproducible high-performance thin-layer chromatographic (HPTLC) method using UV or fluorescence scanning densitometry has been developed for the assay and purity control of methoxymorpholinodoxorubicin hydrochloride (FCE 23762). With a mobile phase of chloroform-methanol-acetic acid (93:6:1, v/v/v) and a silica gel plate, all potential impurities were separated from the main component and from each other. Detection limits at a signal-to-noise ratio of 2:1 were a few nanograms for UV detection and < 1 ng for fluorescence emission. The RSD values for the examined compounds were all < 3%.
Farina
A
A
Istituto Superiore di Sanità, Roma, Italy.
Quaglia
M G
MG
Doldo
A
A
Calandra
S
S
Gallo
F R
FR
D000085:Acetates; D019342:Acetic Acid; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid; D003720:Densitometry; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000032:analysis; D000432:Methanol; D012015:Reference Standards
D000085:Acetates; C066998:methoxy-morpholinyl-doxorubicin; D002725:Chloroform; D004317:Doxorubicin; D019342:Acetic Acid; D000432:Methanol