Responses of tissue ascorbic acid and of serum cholesterol, alpha-tocopherol, and ceruloplasmin in rats to dietary level of cystine.
The present study was conducted with growing rats to examine effects of addition of 0.3, 1.0, 2.5, and 5% L-cystine to 10 or 25% casein diet on tissue ascorbic acid and on serum cholesterol, alpha-tocopherol, and ceruloplasmin activity. Addition of 0.3-1% cystine to 10% casein diet caused the maximum growth and addition of 5% cystine to 25% casein diet depressed the growth. Increases in liver levels of ascorbic acid and in serum levels of cholesterol and alpha-tocopherol were observed with addition of 5% cystine to 10 and 25% casein diets as compared to the diets without addition of cystine. Addition of 0.3-5% cystine to 10% casein diet and addition of 5% cystine to 25% casein diet caused a decreased activity of serum ceruloplasmin. The changes in liver ascorbic acid, serum cholesterol, and serum alpha-tocopherol and in ceruloplasmin activity by dietary cystine correlated with the changes in liver levels of non-protein sulfhydryl.
Yang
B S
BS
Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University, Higashi, Japan.
Katayama
T
T
Kato
N
N
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D002570:Ceruloplasmin / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood*; D003300:Copper / Q000378:metabolism; D003553:Cystine / Q000378:metabolism / Q000494:pharmacology*; D004032:Diet; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000097:blood*
D014810:Vitamin E; D003553:Cystine; D003300:Copper; D002784:Cholesterol; D002570:Ceruloplasmin; D001205:Ascorbic Acid