[Kinetics of luciferase gene expression from fireflies Photinus pyralis and Luciola mingrelica in Escherichia coli cells. II. The role of pH in the biosynthesis of proteins and their transformation into active conformers].
The Photinus pyralis and Luciola mingrelica luciferases genes expression kinetics has been studied, repectively, on the pME61 and pJG lambda plasmids with a thermoinducible lambda PR promoter in the E. coli strain CA under conditions of a pH shift. An increase in the enzyme activity after a pH shift has been revealed. The maximal amount of the firefly Ph. pyralis luciferase reaches 5.3%, while the maximal amount of the L. mingrelica enzyme reaches 4.9% of the total amount of cellular proteins in case of pHout shift to 9.0 according to the SDS gel electrophoresis data; that in case of a pHout shift to 8.5 is 4.9% and 4.5%, respectively. The enzyme activation correlates well with the dynamics of pHout and pHin.
Leont'eva
O V
OV
Kutuzova
G D
GD
Turovetskiĭ
V B
VB
Ugarova
N N
NN
D000818:Animals; D003001:Cloning, Molecular; D001517:Coleoptera / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008156:Luciferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry
D011506:Proteins; D008156:Luciferases
Kinetika ékspressii genov liutsiferaz svetliakov Photinus pyralis i Luciola mingrelica v kletkakh Escherichia coli. II. Rol' pH v biosinteze belkov i ikh transformatsii v aktivnye konfermery.