title: Assessment of the labeling index of cohorts of the anterior pituitary cell population in phenobarbital-treated male rats by a double immunohistochemical technique for bromodeoxyuridine and pituitary hormones.
abstract: A previous study demonstrated that administration of phenobarbital for up to 7 days to male AP Wistar rats caused alterations in labeling indices (LIs) of several different tissues as determined by immunohistochemical visualization of bromodeoxyuridine (BrdU) incorporation into S-phase nuclei. The pivotal role of the pituitary gland in the function of the endocrine system and changes in circulating hormone levels that result from administration of xenobiotics prompted our consideration of the possible changes in LIs of individual cohorts of the anterior pituitary cell population that may occur as a specific functional adaptation during phenobarbital administration. We evaluated the LIs of individual anterior pituitary cell cohorts by modifying a double immunohistochemical staining method for bromodeoxyuridine and pituitary hormones using a sequential peroxidase-anti-peroxidase (PAP)/alkaline phosphatase-anti-alkaline phosphatase (APAAP) method employing diaminobenzidine and New Fuchsin chromogens, respectively. The method was robust and reproducible. Differences were noted in individual anterior pituitary cohort LIs between control and phenobarbital-treated groups, although no statistically significant difference was evident. We conclude that no detectable effects on individual cohort LIs were induced by treatment with phenobarbital for up to 7 days and that any functional adaptation to treatment was associated with increased hormone release. We believe that the visualization, identification, and quantitation of replicating cells in specific hormone-positive cohorts of the anterior pituitary cell population provide opportunities for understanding the influence of xenobiotics and disease processes on pituitary function.
authors.affiliation: Safety of Medicines Department, Zeneca Pharmaceuticals, Alderley Park, Macclesfield, Cheshire, United Kingdom.
authors.forename: H B
authors.initials: HB
authors.lastname: Jones
authors.forename: S J
authors.initials: SJ
authors.lastname: Harbottle
authors.forename: A L
authors.initials: AL
authors.lastname: Bowdler
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000302:isolation & purification; D002455:Cell Division / Q000187:drug effects; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D010634:Phenobarbital / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000254:growth & development*; D010907:Pituitary Hormones / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010907:Pituitary Hormones; D001973:Bromodeoxyuridine; D010634:Phenobarbital