Deletions on the long arm of chromosome 17 in pilocytic astrocytoma.
Pilocytic astrocytomas are the most common astrocytic tumors of childhood and differ clinically and histopathologically from those astrocytomas that affect adults. Studies of adult astrocytic tumors have revealed allelic losses on chromosomes 10, 17p, 19q and alterations in the epidermal growth factor receptor (EGFR) gene. We have previously examined pilocytic astrocytomas for allelic losses on chromosomes 10 and 19q and for amplification of the EGFR gene, but did not detect genomic alterations at these loci. In the present study we assayed 20 pilocytic astrocytomas for loss of allelic heterozygosity of chromosome 17p, including one locus in the p53 tumor suppressor gene. In addition, because pilocytic astrocytomas frequently affect patients with neurofibromatosis type 1 (NF1) and the NF1 gene has been mapped to 17q11.2, we also examined multiple loci on the long arm of chromosome 17. Allelic loss was observed on chromosome 17 in four cases (three sporadic, one NF1); all lost portions of the long arm in chromosome 17, and one tumor lost the short arm as well. One tumor showed an interstitial deletion on the long arm that included the region of the NF1 gene. These data suggest the presence of a tumor suppressor gene on 17q that is associated with pilocytic astrocytomas. A potential candidate for this gene is the NF1 tumor suppressor gene.
von Deimling
A
A
Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Charlestown.
Louis
D N
DN
Menon
A G
AG
von Ammon
K
K
Petersen
I
I
Ellison
D
D
Wiestler
O D
OD
Seizinger
B R
BR
D000293:Adolescent; D000328:Adult; D000483:Alleles; D001254:Astrocytoma / Q000235:genetics / Q000473:pathology*; D001345:Autoradiography; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002648:Child; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17 / Q000648:ultrastructure*; D015342:DNA Probes; D005260:Female; D016514:Genes, Neurofibromatosis 1; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes