The identity of Leishmania isolated from sand flies and vertebrate hosts in a major focus of cutaneous leishmaniasis in Baturite, northeastern Brazil.
During a field investigation carried out in Baturite, Brazil from 1989 to 1991, sand flies, sympatric rodents, domestic dogs and humans were surveyed for leishmaniasis. Twenty strains of Leishmania were isolated by in vitro culture from Lutzomyia whitmani, three strains were obtained from Rattus rattus, two strains from dogs, and five strains from humans. The isolates were characterized by isoenzyme electrophoresis by hybridization with kinetoplast DNA-specific probes. All the samples were identified as L. (Viannia) braziliensis. The importance of these results in the dynamics of the Leishmania infection in this focus is discussed.
Vasconcelos
I A
IA
Nucleo de Medicina Tropical, Universidade Federal do Ceara, Fortaleza, Brazil.
Vasconcelos
A W
AW
Fe Filho
N M
NM
Queiroz
R G
RG
Santana
E W
EW
Bozza
M
M
Sallenave
S M
SM
Valim
C
C
David
J R
JR
Lopes
U G
UG
D000818:Animals; D001938:Brazil; D015342:DNA Probes; D018105:DNA, Kinetoplast / Q000032:analysis; D004197:Disease Reservoirs; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology*; D007527:Isoenzymes / Q000032:analysis; D007892:Leishmania braziliensis / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology* / Q000635:transmission; D009115:Muridae / Q000469:parasitology; D009693:Nucleic Acid Hybridization; D011576:Psychodidae / Q000469:parasitology*; D051381:Rats; D012376:Rodent Diseases / Q000469:parasitology; D012377:Rodentia
D015342:DNA Probes; D018105:DNA, Kinetoplast; D007527:Isoenzymes