The DNA region around the c-myc gene and its amplification in human tumour cell lines.
In order to study amplicons containing the c-myc locus in human tumour cells we have analysed the region around the human c-myc gene. Using pulsed field gel electrophoresis we have constructed a 1.8 Mb map of the c-myc locus using six rare-cutting endonucleases. In addition we have cloned 130 kb of continuous DNA around the c-myc gene which includes 65 kb of 5' sequence and 65 kb of 3' sequence. The first exon of the pvt-1 gene was located 59 kb 3' of the c-myc gene. Using nine neighbouring probes, covering a region of about 395 kb around the c-myc gene, we were able to determine the extent and composition of c-myc amplicons in the HL60, NCI-N417, Colo320HSR, SkBr3 and the Sk-N-MC human tumour cell lines. Furthermore we analysed the chromosomal location of amplified c-myc DNA in NCI-N417 and SkBr3 cells. Similarities and differences between the amplified DNA in the different cell lines are discussed.
Feo
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Istituto di Biologia dello Sviluppo C.N.R., Palermo, Italy.
Di Liegro
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C
Jones
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Read
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Fried
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D003001:Cloning, Molecular; D004247:DNA; D005784:Gene Amplification; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D004247:DNA