Leucine synthesis in spinach chloroplasts: partial characterization of 2-isopropylmalate synthase.
Leucine synthesis was found to be localized in chloroplasts of higher plants. The introductory enzyme of the isopropylmalate pathway, the 2-isopropylmalate synthase, was strongly feedback-regulated by leucine. The pH optimum of the enzyme was between 7.0 and 9.0. The Km values were 5 microM for acetyl-CoA and 75 microM for 2-oxoisovalerate.
Hagelstein
P
P
Botanisches Institut, Tierärztlichen Hochschule Hannover, Germany.
Schultz
G
G
D015079:2-Isopropylmalate Synthase / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007930:Leucine / Q000096:biosynthesis*; D010944:Plants / Q000201:enzymology / Q000378:metabolism*
D015079:2-Isopropylmalate Synthase; D007930:Leucine