Determination of tris(hydroxymethyl)aminomethane (tromethamine) in human plasma and urine by high-performance liquid chromatography with fluorescence detection.
Determination of tromethamine (2-amino-2-hydroxymethyl-1,3-propanediol) in human plasma and urine has been developed and validated. The method utilizes reversed-phase high-performance liquid chromatographic separation and fluorescence detection of a pre-column derivative. The assay was linear in the ranges of 1-200 micrograms/ml plasma and 5-500 micrograms/ml urine with accuracies (mean percent recovery) all within 10% of 100% and precisions (coefficient of variation) all less than 10%.
Morris
M J
MJ
Merck Research Laboratories, West Point, PA 19486.
Hsieh
J Y
JY
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013050:Spectrometry, Fluorescence; D014325:Tromethamine / Q000032:analysis*
D014325:Tromethamine