title: Bovine seminal ribonuclease destabilizes negatively charged membranes.
abstract: Bovine seminal ribonuclease (BS-RNase), an antitumor protein selectively cytotoxic for malignant cells, (i) specifically aggregates negatively charged vesicles and modifies the thermotropic behaviour of the phospholipid; (ii) decreases the amplitude of the thermal transition of the phospholipid; and (iii) provokes lipid-mixing between bilayers of negatively charged vesicles. This engenders leakage of the aqueous vesicle contents. Monomeric BS-RNase, devoid of antitumor action, does not produce these effects. These results suggest that the destabilization of the membrane bilayer promoted by BS-RNase may be involved in the antitumor action of the protein.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Química, Universidad Complutense, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Mancheño
authors.forename: M
authors.initials: M
authors.lastname: Gasset
authors.forename: M
authors.initials: M
authors.lastname: Oñaderra
authors.forename: J G
authors.initials: JG
authors.lastname: Gavilanes
authors.forename: G
authors.initials: G
authors.lastname: D'Alessio
mesh_terms: D000818:Animals; D002417:Cattle; D004722:Endoribonucleases / Q000737:chemistry*; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000737:chemistry*; D008561:Membrane Fusion; D008563:Membrane Lipids / Q000737:chemistry; D008567:Membranes, Artificial; D012661:Semen / Q000201:enzymology
chemical_list: D008051:Lipid Bilayers; D008563:Membrane Lipids; D008567:Membranes, Artificial; D004722:Endoribonucleases; C032523:ribonuclease SPL