Measurement of the vasoconstrictive substances endothelin, angiotensin II, and thromboxane B2 in cold storage solution can reveal previous renal ischemic insults.
In a rat model, the left kidney was subjected to 60 min of normothermic ischemia followed by 15 min of reperfusion, whereas the right kidney, serving as a paired control, was not rendered ischemic. Both kidneys were then perfused in situ with either Euro-Collins (EC) solution (n = 12) or University of Wisconsin (UW) solution (n = 6) for 10 min. Each kidney was then harvested and stored at 4 degrees C in its respective solution. After 24 and 48 h of cold storage, the following vasoactive substances were measured in the preservation media: endothelin (ET), angiotensin II (A-II), thromboxane (B2) (TxB2), and prostaglandin I2 (PGI2). After 24 h in EC solution, left kidneys uniformly produced significantly higher concentrations of each vasoactive substance than right kidneys: ET 1.64 +/- 0.3 pg/ml vs 0.82 +/- 0.1 pg/ml (P < or = 0.009); A-II 20.8 +/- 6.2 pg/ml vs 7.75 + 2.3 pg/ml (P < or = 0.007); TxB2 100.8 +/- 17.7 pg/ml vs 40.1 +/- 11.7 pg/ml (P < or = 0.04); PGI2 638.3 +/- 41.1 pg/ml vs 318.3 +/- 36.4 pg/ml (P < or = 0.001), respectively. At 48 h, a similar pattern of results was obtained as the kidney continued to produce TxB2 and prostacyclins during the 24-48 h period. In the UW solution, basal levels of ET and A-II were lower than those in EC solution, but similarly increased after initial ischemia.(ABSTRACT TRUNCATED AT 250 WORDS)
Gianello
P
P
Laboratory of Experimental Surgery, University of Louvain Medical School, Brussels, Belgium.
Fishbein
J
J
Besse
T
T
Gustin
T
T
Chatzopoulos
C
C
Ketelslegers
J M
JM
Lambotte
L
L
Squifflet
J P
JP
D000241:Adenosine / Q000737:chemistry; D000493:Allopurinol / Q000737:chemistry; D000804:Angiotensin II / Q000032:analysis*; D000818:Animals; D015925:Cryopreservation; D016232:Endothelins / Q000032:analysis*; D005978:Glutathione / Q000737:chemistry; D006801:Humans; D006982:Hypertonic Solutions / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D007511:Ischemia / Q000175:diagnosis* / Q000378:metabolism; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism; D016030:Kidney Transplantation; D008297:Male; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011863:Radioimmunoassay; D011887:Raffinose / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013929:Thromboxane B2 / Q000032:analysis*
D016232:Endothelins; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000804:Angiotensin II; D013929:Thromboxane B2; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose