title: Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR.
abstract: A single-tube nested PCR able to discriminate between ocular infection with Chlamydia trachomatis of serovar A, B, or C is described. The method uses genotype-specific primers labeled with different fluorochromes, "drop-in/drop-out" PCR amplification, and product analysis on an Applied Biosystems 373A DNA Sequencer using GENESCAN 672 software. The system readily detected mixed infection with serotypes A and B within the same eye. This strategy provides a rapid genotyping method applicable to a wide variety of epidemiological studies.
authors.affiliation: Department of Molecular Microbiology, University Medical School, Southampton General Hospital, UK.
authors.forename: S
authors.initials: S
authors.lastname: Pecharatana
authors.forename: M A
authors.initials: MA
authors.lastname: Pickett
authors.forename: P J
authors.initials: PJ
authors.lastname: Watt
authors.forename: M E
authors.initials: ME
authors.lastname: Ward
mesh_terms: D001483:Base Sequence; D002690:Chlamydia Infections / Q000453:epidemiology / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D015818:Eye Infections, Bacterial / Q000453:epidemiology / Q000382:microbiology*; D005456:Fluorescent Dyes; D005714:Gambia / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes