title: Immunocytochemical and ultrastructural characterization of endocrine cells and nerves in the intestine of Rana temporaria.
abstract: Endocrine cells have been identified in the intestine of the frog Rana temporaria after application of the Grimelius and Masson-Fontana techniques. These endocrine cells were examined using immunocytochemical techniques on paraffin and semithin sections for light microscopy. After testing 19 antisera, 12 immunoreactivities were identified. Numerous serotonin-, somatostatin- and GLP-1-immunoreactive cells; a moderate number of PYY-, glucagon-, VIP-, gastrin/CCK-immunoreactive cells and few human PP-, bombesin-, substance P- and neurotensin-immunoreactive cells were found. VIP- and met-enkephalin were identified in nerve fibers of the muscular layer. Using semithin-thin sections five types of endocrine cells (serotonin-, somatostatin-, gastrin/CCK-, glucagon- and bombesin-immunoreactive cells) have been characterized according to their immunocytochemical reaction and the ultrastructure of the secretory granules.
authors.affiliation: Department of Histology and Pathology, University of Navarra, Pamplona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Valverde
authors.forename: O
authors.initials: O
authors.lastname: Díaz de Rada
authors.forename: M A
authors.initials: MA
authors.lastname: Burrell
authors.forename: J
authors.initials: J
authors.lastname: Rovira
authors.forename: P
authors.initials: P
authors.lastname: Sesma
mesh_terms: D001078:APUD Cells / Q000737:chemistry* / Q000648:ultrastructure; D000818:Animals; D001839:Bombesin / Q000032:analysis; D005934:Glucagon / Q000032:analysis; D007124:Immunoenzyme Techniques; D007422:Intestines / Q000166:cytology / Q000294:innervation*; D008854:Microscopy, Electron; D011896:Rana temporaria; D012701:Serotonin / Q000032:analysis; D013004:Somatostatin / Q000032:analysis
chemical_list: D012701:Serotonin; D013004:Somatostatin; D005934:Glucagon; D001839:Bombesin