title: Dietary regulation of rat intestinal angiotensin-converting enzyme and dipeptidyl peptidase IV.
abstract: The small intestinal brush-border membrane contains several peptidases that are involved in the hydrolysis of dietary peptides containing proline. A high-proline (gelatin) diet was administered to one of several groups of rats to study its possible regulatory effect on levels of two prolyl peptidases, namely angiotensin-converting enzyme (ACE) and dipeptidyl peptidase IV (DPP IV). Groups of rats were maintained on isocaloric diets containing either low (4%), normal (17%), or high (50%) protein (casein) or high (50%) gelatin. After 7 days, brush-border membranes and total RNA were prepared from the small intestine. ACE activity was 3- to 10-fold higher in brush-border membranes from the gelatin group compared with the low-protein group. DPP IV exhibited a three- to sixfold increase. Immunoblot analysis of brush-border membrane-associated ACE protein indicated a six- to eightfold increase in the high-gelatin group. There was also a 1.5- to 3-fold increase in steady-state levels of ACE and DPP IV mRNA. These results suggest that a diet high in proline (gelatin) is particularly effective in increasing intestinal levels of these two enzymes.
authors.affiliation: Gastrointestinal Research Laboratory, Department of Veterans Affairs Medical Center, San Francisco 94121.
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: R H
authors.initials: RH
authors.lastname: Erickson
authors.forename: A
authors.initials: A
authors.lastname: Sedlmayer
authors.forename: S K
authors.initials: SK
authors.lastname: Chang
authors.forename: Y
authors.initials: Y
authors.lastname: Ikehara
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001835:Body Weight; D004032:Diet; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting; D007422:Intestines / Q000201:enzymology*; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D012333:RNA, Messenger; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4; D007703:Peptidyl-Dipeptidase A