title: Molecular epidemiology of human rotaviruses in Manipur: genome analysis of rotaviruses of long electropherotype and subgroup I.
abstract: In 1987/88 a winter outbreak of infantile gastroenteritis occurred in Manipur, India which was mainly due to rotaviruses of long electropherotype and subgroup (SG) I. The VP7 gene of one of these viruses (M48) has been cloned and sequenced. It was found to be very closely related to the VP7 genes of the G2 serotype human rotaviruses RV-5 and S2. Follow-up epidemiology of this event in Manipur during 1989-1992 yielded mainly rotaviruses of more conventional characteristics (94 isolates of SG II and long electropherotype, and 90 isolates of SG I and short electropherotype), but also 6 isolates of SG I with long electropherotype, indicating that these viruses continue to circulate in the Manipur community. One isolate of short electropherotype was of subgroup II, and one long electropherotype isolate reacted with the group A but not with either the subgroup I or subgroup II monoclonal antibodies.
authors.affiliation: Clinical Microbiology and Public Health Laboratory, University of Cambridge, U.K.
authors.forename: T
authors.initials: T
authors.lastname: Krishnan
authors.forename: B
authors.initials: B
authors.lastname: Burke
authors.forename: S
authors.initials: S
authors.lastname: Shen
authors.forename: T N
authors.initials: TN
authors.lastname: Naik
authors.forename: U
authors.initials: U
authors.lastname: Desselberger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002675:Child, Preschool; D004279:DNA, Viral; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000382:microbiology; D012621:Seasons; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; C050779:VP7 protein, Rotavirus