Potentiation of glucocorticoid-mediated induction of the mouse mammary tumor virus promoter in mammary carcinoma cells by activation of protein kinase C.
In a mammary carcinoma cell line, the glucocorticoid induction of a stably integrated mouse mammary tumor virus-luciferase reporter gene is potentiated up to ten-fold by treatment of the cells with phorbol ester tumor promoters. This potentiation is stereospecific and is observed with a structurally unrelated tumor promoter that also activates protein kinase C. Another tumor promoter, thapsigargin, that acts via mobilization of intracellular calcium stores, fails to potentiate the hormone response. This potentiation contrasts to the inhibition or lack of effect observed in fibroblasts. Cell specific interactions of different signal transduction pathways with steroid response pathways may play an important role in determining the tissue specificity of steroid hormone action.
Nordeen
S K
SK
University of Colorado Health Sciences Center, Dept. of Pathology, Denver 80262-0216.
D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D007211:Indoles / Q000494:pharmacology; D007769:Lactams / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental; D008324:Mammary Tumor Virus, Mouse / Q000187:drug effects / Q000235:genetics*; D051379:Mice; D010703:Phorbol Esters / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism*; D013729:Terpenes / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D019284:Thapsigargin; D014407:Tumor Cells, Cultured
D007211:Indoles; D007769:Lactams; D010703:Phorbol Esters; D013729:Terpenes; C086014:7-octylindolactam V; C029704:12-deoxyphorbolphenylacetate; D019284:Thapsigargin; D003907:Dexamethasone; C080287:thymeleatoxin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate