Comparison of the effects of interleukin-1 beta on proteoglycan synthesis by human skin and post-burn normal scar explant cultures.
The effect of interleukin (IL)-1 beta on proteoglycan (PG) synthesis and secretion, into culture medium by normal human skin and post-burn human normal scar using tissue explants in culture, was investigated. Following exposure of different tissues to labeling with Na2[35SO4] in the presence and absence of IL-1 beta, the extractable [35SO4]PG (isolated from 0.15 M NaCl and 4 M Gdm. Cl extracts), non-extractable [35SO4]PG (isolated after papain treatment of residual tissue), and [35SO4]PG secreted into culture medium were analyzed for contents and distribution. The contents of [35SO4]PG as measured by [35SO4] incorporation indicate differences in [35SO4]PG production of extractable and non-extractable PGs and also in the PGs released into the culture medium. Examination of the sizes of [35SO4]PGs on Sepharose CL-6 beta columns with and without treatment of IL-1 beta shows that the size of non-extractable [35SO4]PG decreases after IL-1 beta treatment. Cellulose acetate plate electrophoresis of these [35SO4]PG fractions shows that the distribution of PGs alters after treatment with IL-1 beta. These results indicate that burn wound healing abnormalities (scarring) is related to a change in the level of PGs, and may be modified by IL-1 beta treatment.
Garg
H G
HG
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Massachusetts General Hospital, Boston.
Lippay
E W
EW
Carter
E A
EA
Donelan
M B
MB
Remensnyder
J P
JP
Siebert
J W
JW
D000293:Adolescent; D002056:Burns / Q000150:complications*; D002921:Cicatrix / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008875:Middle Aged; D009924:Organ Culture Techniques; D011509:Proteoglycans / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D007375:Interleukin-1; D011509:Proteoglycans; D011994:Recombinant Proteins