title: Identification of low-molecular-mass coproantigens of Cryptosporidium parvum.
abstract: Cryptosporidium parvum coproantigens (CCAg) of 18 and 20 kDa were identified in the stool eluates of calves and humans infected with Cryptosporidium species. Monospecific antibodies raised against the 20-kDa antigen recognized both 18- and 20-kDa CCAg in all positive but no negative control samples. These antibodies reacted with C. parvum sporozoites in an immunofluorescence assay. Human immune sera recognized the 20-kDa antigen in infected calf stool eluates. Both 18- and 20-kDa CCAg remained intact in commonly used preservatives and at various temperatures. These CCAg may be useful in designing sensitive, reliable methods for diagnosing cryptosporidiosis.
authors.affiliation: Division of Pediatric Infectious Diseases, University of Alberta, Edmonton, Canada.
authors.forename: K
authors.initials: K
authors.lastname: el-Shewy
authors.forename: R T
authors.initials: RT
authors.lastname: Kilani
authors.forename: M M
authors.initials: MM
authors.lastname: Hegazi
authors.forename: L M
authors.initials: LM
authors.lastname: Makhlouf
authors.forename: W M
authors.initials: WM
authors.lastname: Wenman
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry*; D015153:Blotting, Western; D002417:Cattle; D003429:Cross Reactions; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000276:immunology*; D016785:Cryptosporidium parvum / Q000276:immunology*; D003967:Diarrhea / Q000469:parasitology; D006801:Humans; D008970:Molecular Weight
chemical_list: D000953:Antigens, Protozoan