Direct determination of mitoxantrone in plasma by high performance liquid chromatography using an automatic precolumn-switching system as sample clean-up procedure.
A high-performance liquid chromatography method which uses direct injection and a column-switching valve for determination of mitoxantrone in plasma is described. After addition of internal standard, plasma was deproteinized by adding 5-sulphosalicylic acid reagent. The supernatant was injected onto an enrichment precolumn flushed with washing solvent (methanol and water 5:95). Absorbed mitoxantrone was backflushed from the precolumn into an analytical column C18 Nucleosil 250 x 4 mm with a gradient elution (solvent A, ammonium formate buffer 1.6 M, pH 4.3; solvent B, acetonitrile and water 40:60; linear gradient from 45 to 55% of B for 30 min was programmed) at a flow rate of 1.3 mL/min. Detection was carried out at 665 nm. This method showed obvious advantages over conventional extraction procedures in terms of speed and facility of sample handling.
Catalin
J
J
Laboratoire de Toxicocinétique et de Pharmacocinétique Faculté de Pharmacie, Marseille, France.
Peloux
A F
AF
Coloma
F
F
Payet
B
B
Lacarelle
B
B
Cano
J P
JP
Just-Landi
S
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008942:Mitoxantrone / Q000097:blood*; D015203:Reproducibility of Results
D008942:Mitoxantrone