Adhesion of CD16+ K cells to antibody-coated targets is mediated by CD2 and CD18 receptors.
In order to investigate the function of CD2 and CD18 receptors in antibody-dependent cellular cytotoxicity (ADCC), Fab' fragments of the monoclonal antibodies 9.6 (anti-CD2) and 60.3 (anti-CD18) were preincubated with the human natural killer (NK) clone EB4.19 and tested for conjugate formation and cytotoxic function against the human B-cell line KMS and the mouse thymoma line SL-2. We concluded that: (1) the FcR CD16 does not participate in conjugate formation; (2) adhesion between target and effector cells mediated by CD2 and CD18 is a prerequisite for subsequent activation of the lytic programme through the CD16 receptor.
Voltarelli
J C
JC
Fred Hutchinson Cancer Research Center, Seattle, Washington.
Gjerset
G
G
Anasetti
C
C
D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D018821:CD18 Antigens; D018801:CD2 Antigens; D002448:Cell Adhesion / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D017452:Receptors, IgG / Q000032:analysis*; D011971:Receptors, Immunologic / Q000276:immunology*; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018821:CD18 Antigens; D018801:CD2 Antigens; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D016028:Receptors, Leukocyte-Adhesion