Despite its high representation in extrachromosomal circular DNAs from Drosophila embryos, the dodecasatellite does not allow autonomous replication in cultured cells.
The dodecasatellite is a 11/12 bp tandemly repeated sequence which is overrepresented, with regard to its genomic representation, in extrachromosomal circular DNAs from D melanogaster embryos. Here we show that a bacterial plasmid carrying a cluster of dodecasatellite is not able to replicate efficiently in cultured cells. This observation does not support the hypothesis that the overrepresentation results from an autonomous replication of dodecasatellite circular DNA molecules.
Renault
S
S
Laboratoire de Génétique, URA 360 CNRS, Université Blaise-Pascal, Aubière, France.
Degroote
F
F
Picard
G
G
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004261:DNA Replication; D004270:DNA, Circular; D004276:DNA, Satellite; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D005111:Extrachromosomal Inheritance; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid
D004270:DNA, Circular; D004276:DNA, Satellite; D009838:Oligodeoxyribonucleotides