A new 2D NMR method for measurement of JHH coupling constants.
A new 2D NMR pulse sequence for E.COSY-type measurement of J(HH) coupling constants is introduced. It exploits a heteronuclear spin, e.g., 13C, for displacement in the omega(1) frequency dimension via a large heteronuclear J coupling. The experiment is demonstrated by application to a heptapeptide at the natural abundance 13C level. It is suitable, for example, for measurement of 3J(HH) and 4J(HH) coupling constants in peptides and proteins.
Sørensen
M D
MD
Department of Chemistry, University of Copenhagen, Denmark.
Led
J J
JJ
Sørensen
O W
OW
D000595:Amino Acid Sequence; D007328:Insulin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry
D007328:Insulin; D010446:Peptide Fragments