Long-range cis preference in DNA homology search over the length of a Drosophila chromosome.
P element-induced chromosome breakage on the X chromosome of Drosophila melanogaster was repaired six times more frequently when a homologous template was located anywhere on the X chromosome rather than on an autosome. Cis-trans comparisons confirmed that recombinational repair was more frequent when the interacting sequences were physically connected. These results suggest that the search for homology between the broken ends and a matching template sequence occurs preferentially in the cis configuration. This cis advantage operates over more than 15 megabases of DNA.
Engels
W R
WR
Genetics Department, University of Wisconsin, Madison 53706.
Preston
C R
CR
Johnson-Schlitz
D M
DM
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004260:DNA Repair; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005785:Gene Conversion; D008297:Male; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013698:Templates, Genetic; D014960:X Chromosome
D004251:DNA Transposable Elements; D004247:DNA