Transactivation of the 'promoterless' luciferase-encoding vectors pXP1 and pXP2 by C/EBP alpha.
Transactivation of the firefly luciferase-encoding vectors, pXP1 and pXP2, by the alpha isoform of CCAAT/enhancer-binding protein (C/EBP) is reported. Thus, these vectors are not 'promoterless' in every cellular context, and transactivation by C/EBP or a closely related factor should be considered as a possible explanation for the relatively high background levels of luciferase production that are occasionally observed after transfection of certain cells with these or other similar vectors.
Li
Y C
YC
Biotechnology Center, Ohio State University, Columbus 43210.
Hayes
S
S
Young
A P
AP
D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D001517:Coleoptera / Q000201:enzymology / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors; D009687:Nuclear Proteins / Q000378:metabolism*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012160:Retina / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation; D014162:Transfection
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors