Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses.
The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.
Zhang
X
X
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge.
Herbst
W
W
Kousoulas
K G
KG
Storz
J
J
D000372:Agglutination Tests; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002460:Cell Line; D002645:Chickens; D018352:Coronavirus Infections / Q000382:microbiology / Q000662:veterinary*; D017938:Coronavirus, Bovine / Q000235:genetics* / Q000276:immunology; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral; D004751:Enteritis / Q000382:microbiology / Q000662:veterinary; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D008171:Lung Diseases / Q000382:microbiology / Q000662:veterinary; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000235:genetics*
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus