A simple fluorimetric method for the estimation of ligand binding parameters in ligand-enzyme complexes.
The theory and practice of a simple fluorimetric method for the estimation of ligand binding parameters in ligand-enzyme complexes is described. In this method, the concentration of a ligand-binding enzyme and the dissociation constant of a ligand-enzyme complex were estimated solely from the total concentration of a ligand and the total fluorescence of the ligand in the absence and in the presence of enzyme.
Leskovac
V
V
Faculty of Technology, Bulevar AVNOJa 1, Novi Sad, Yugoslavia.
Trivić
S
S
Pantelić
M
M
D000426:Alcohol Dehydrogenase / Q000737:chemistry*; D005470:Fluorometry; D008024:Ligands; D009243:NAD / Q000737:chemistry*; D015203:Reproducibility of Results; D017186:Titrimetry
D008024:Ligands; D009243:NAD; D000426:Alcohol Dehydrogenase