Mineralization of p-methyl-14C-benzoate in soils by Pseudomonas putida (pWW0).
Pseudomonas putida bearing the archetypal TOL plasmid pWW0 metabolizes p-methylbenzoate through a meta-cleavage pathway. In complex environments such as soils that are relatively rich in organic matter, we observed metabolism of p-methyl-14C-benzoate, which could be monitored as 14CO2 evolution from the labelled alkylaromatic. Linear 14CO2 evolution in soils took place for at least a month, although efficient mineralization of the alkylaromatic required appropriate mass transfer to allow the bacterial cell and target chemical to remain in close contact.
Duque
E
E
Department of Plant Biochemistry, C.S.I.C.-Estación Experimental del Zaidin, Granada, Spain.
Marqués
S
S
Ramos
J L
JL
D001565:Benzoates / Q000378:metabolism*; D002250:Carbon Radioisotopes; D008903:Minerals / Q000378:metabolism*; D016958:Pseudomonas putida / Q000737:chemistry / Q000378:metabolism*; D012988:Soil Microbiology
D001565:Benzoates; D002250:Carbon Radioisotopes; D008903:Minerals; C009504:4-toluic acid