title: Mycobacterium celatum sp. nov.
abstract: A new slowly growing nonphotochromogenic Mycobacterium species of clinical importance is described. The biochemical characteristics of this organism were similar to those of Mycobacterium xenopi and members of the Mycobacterium avium complex. However, none of the strains reacted with commercially available genetic probes for the M. avium complex. The strains were resistant to most antituberculosis drugs. Multilocus enzyme electrophoresis revealed two original electrophoretic types, which was suggestive of new species. The strains contained alpha-, keto-, and dicarboxylic mycolates, as determined by thin-layer chromatography. A mycolic acid analysis by high-performance liquid chromatography revealed a chromatographic pattern similar to that of M. xenopi, but distinct from the patterns of previously described Mycobacterium species. Hexadecanoic and tuberculostearic acids were identified as the major cell wall fatty acids by gas-liquid chromatographic analysis; hexacosanoic acid was the major mycolic acid cleavage product, and 2-eicosanol was the major alcohol. Evaluation of the 16S rRNA sequence confirmed the phylogenetic position of the organism among the slowly growing Mycobacterium species. Cultures representing this new species have been deposited in the American Type Culture Collection as strains ATCC 51130 and ATCC 51131T (T = type strain). The name Mycobacterium celatum is proposed.
authors.affiliation: Division of Bacterial and Mycotic Diseases, Centers for Diseases Control and Prevention, Atlanta, Georgia 30333.
authors.forename: W R
authors.initials: WR
authors.lastname: Butler
authors.forename: S P
authors.initials: SP
authors.lastname: O'Connor
authors.forename: M A
authors.initials: MA
authors.lastname: Yakrus
authors.forename: R W
authors.initials: RW
authors.lastname: Smithwick
authors.forename: B B
authors.initials: BB
authors.lastname: Plikaytis
authors.forename: C W
authors.initials: CW
authors.lastname: Moss
authors.forename: M M
authors.initials: MM
authors.lastname: Floyd
authors.forename: C L
authors.initials: CL
authors.lastname: Woodley
authors.forename: J O
authors.initials: JO
authors.lastname: Kilburn
authors.forename: F S
authors.initials: FS
authors.lastname: Vadney
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000453:epidemiology / Q000382:microbiology*; D009171:Mycolic Acids / Q000032:analysis; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014481:United States / Q000453:epidemiology
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D006360:Heat-Shock Proteins; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S