Interferon-gamma-inducible endothelial cell class II major histocompatibility complex expression correlates with strain- and site-specific susceptibility to experimental allergic encephalomyelitis.
The interaction between encephalitogenic lymphocytes and the cerebral microvascular lining is considered to be an important initial step in the recruitment of immune cells into the central nervous system (CNS) under pathological conditions such as multiple sclerosis (MS) and its investigative analog, experimental allergic encephalomyelitis (EAE). This study was conducted in order to examine whether differences in microvascular endothelial cell expression of several molecules involved in lymphovascular interactions correlate with the strain and organ-specific development of EAE. Cerebral and epididymal microvascular endothelial cells (EC) were isolated from SJL and B10.S mice, which, despite MHC-compatibility (H-2S), differ in their ability to develop EAE. The subcultured cells were then analyzed by flow cytometry for their ability to express class I MHC, class II MHC and ICAM-1 molecules in response to treatment with murine recombinant interferon-gamma (IFN-gamma). Over a range of doses and times, cerebral EC cultures derived from EAE-susceptible SJL mice expressed two-fold higher levels and higher cell surface densities of class II molecules than cerebral EC cultures derived from EAE-resistant B10.S mice, whereas class I and ICAM-1 molecules were comparably upregulated on both SJL and B10.S cerebral EC. In contrast, both SJL and B10.S epididymal EC cultures expressed lower but comparable levels of class II molecules in response to IFN-gamma. Class I and ICAM-1 molecules, however, were upregulated to at least the same degree as that observed on cerebral EC derived from both strains.(ABSTRACT TRUNCATED AT 250 WORDS)
Jemison
L M
LM
Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA.
Williams
S K
SK
Lublin
F D
FD
Knobler
R L
RL
Korngold
R
R
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D001921:Brain / Q000098:blood supply; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D004198:Disease Susceptibility; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics* / Q000276:immunology; D004730:Endothelium, Vascular / Q000166:cytology; D004822:Epididymis / Q000098:blood supply; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D005805:Genes, MHC Class I / Q000235:genetics; D005802:Genes, MHC Class II / Q000235:genetics*; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000502:physiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D015854:Up-Regulation
D000945:Antigens, Differentiation, T-Lymphocyte; D015815:Cell Adhesion Molecules; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma