title: The gene encoding the kappa-carrageenase of Alteromonas carrageenovora is related to beta-1,3-1,4-glucanases.
abstract: The nucleotide (nt) and deduced amino acid (aa) sequences are reported for the structural gene cgkA encoding kappa-carrageenase (M(r) 44,412) from the marine bacterium Alteromonas carrageenovora (ATCC 43555), a hydrolase involved in the degradation of kappa-carrageenan. The cgkA gene codes for a protein of 397 aa, with a signal peptide of 25 aa. The enzyme is a new member of family 16 of glycosyl hydrolases, which comprises beta-1,3-1,4-glucanases from various sources and the beta-agarase of Streptomyces coelicolor. It is proposed that residue Glu163 in the kappa-carrageenase from A. carrageenovora and Glu155 in the beta-agarase from S. coelicolor are important for catalysis.
authors.affiliation: Centre d'Etudes d'Océanologie et de Biologie Marine, CNRS UPR 4601, Roscoff, France.
authors.forename: T
authors.initials: T
authors.lastname: Barbeyron
authors.forename: B
authors.initials: B
authors.lastname: Henrissat
authors.forename: B
authors.initials: B
authors.lastname: Kloareg
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000235:genetics*; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D006026:Glycoside Hydrolases; C028020:licheninase; C019624:agarase; C019846:kappa-carrageenase protein, Alteromonadaceae