Interactions between cultured bovine arterial endothelial and smooth muscle cells: studies on uptake and degradation of low density lipoproteins by smooth muscle cells.
This study was designed to investigate the effects of substances released from non-injured and injured bovine arterial endothelial cells on 125I-low density lipoprotein uptake and degradation by smooth muscle cells in culture. It was demonstrated that endothelial cell-released non-dialysable (molecular weight cut off 12-14000) substances significantly stimulated 125I-low density lipoprotein uptake and degradation by smooth muscle cells. Endothelial cell-released dialysable substances and endothelin-1 did not cause this stimulation. The increase in 125I-low density lipoprotein uptake and degradation by smooth muscle cells could be dissociated from cell proliferation. However, in endothelial cell-smooth muscle cell co-culture 125I-low density lipoprotein uptake and degradation by smooth muscle cells were not stimulated. Injury to endothelial cells by lipid-soluble smoke particles or ultraviolet light, which reduced total cellular protein by 15-25%, enhanced the endothelial cell release of the substances stimulating 125I-low density lipoprotein uptake. The results are discussed in relation to atherogenesis.
Xu
C B
CB
Department of Medicine, University of Lund, Malmö General Hospital, Sweden.
Stavenow
L
L
Liao
W
W
Erlinge
D
D
Edvinsson
L
L
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000378:metabolism; D001381:Azepines / Q000494:pharmacology; D001798:Blood Proteins / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D065128:Endothelin Receptor Antagonists; D016232:Endothelins / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D011833:Radiation Injuries, Experimental; D012906:Smoke; D013936:Thymidine / Q000494:pharmacology
D001381:Azepines; D001798:Blood Proteins; D065128:Endothelin Receptor Antagonists; D016232:Endothelins; D007211:Indoles; D008077:Lipoproteins, LDL; D012906:Smoke; C079574:FR 139317; D013936:Thymidine