Constitutive expression of Ly-6.A2 on murine keratinocytes and inducible expression on TCR gamma delta+ dendritic epidermal T cells.
We investigated the expression of Ly-6.A2 on isolated murine epidermal cells by flow cytometry. Ly-6.A2 was expressed on 61% of keratinocytes and 6% of dendritic epidermal T cells of C57BL mice. Phosphatidylinositol-specific phospholipase C removed Ly-6.A2, indicating that the antigen is anchored to the keratinocyte membrane via a glycosyl-phosphatidylinositol anchor similar to its attachment to the membrane of lymphocytes. Induction of dermatitis by topical application of PMA increased the expression of Ly-6.A2 on TCR gamma delta+ dendritic epidermal T cells and did not change its expression on keratinocytes. The increased expression of Ly-6.A2 on dendritic epidermal T cells was transient and reached a peak at 4 days after application of PMA, when 55% of the cells were positive.
HogenEsch
H
H
Department of Pathology and Immunology, TNO-Institute for Aging and Vascular Research, Leiden, The Netherlands.
de Geus
B
B
Tielen
F
F
Rozing
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis; D002462:Cell Membrane / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry / Q000276:immunology; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018800:Thy-1 Antigens; D015854:Up-Regulation / Q000235:genetics
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018800:Thy-1 Antigens; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D013755:Tetradecanoylphorbol Acetate