Effects of the Nd:YAG laser and combined treatments on in vitro fibroblast attachment to root surfaces.
The purpose of this in vitro study was to evaluate the effects of the Nd:YAG laser either alone or in combination with root planning or air-powder abrasive treatment on fibroblast attachment to non-diseased root surfaces. 28, 4 x 4 mm root specimens and four disc-shaped root specimens 6 mm in diameter were obtained from unerupted 3rd molars. The root segments were randomly assigned to 4 treatment groups: (1) control; (2) laser-only treated; (3) laser treated followed by root planning; (4) laser treated followed by air-powder abrasive treatment. Laser-treated root specimens were exposed for 1 min with the Nd:YAG laser calibrated at an energy setting of 75 mJ at 20 pulses/s using a 320 microns contact fiber. The contact fiber was held parallel to the root segments and the root segments were kept moist with distilled water. Following the prescribed treatments, the root specimens were incubated with fibroblast cultures and then prepared for SEM examination. Results of cell counts of fibroblasts attached to specimens within each treatment group yielded the following means and standard deviations: control groups, 181.64 +/- 44.74; lased only, 78.57 +/- 21.35; lased and root planed 125.35 +/- 26.13; and lased followed by an air-powder abrasive, 177.28 +/- 55.71. Application of ANOVA followed by the Dunn Multiple Comparison test revealed significant differences (p < 0.01) in the number of attached cells between the control and laser-only treated groups; and between the laser-only and laser/air-powder abrasive treated groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Thomas
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D
Department of Periodontics, University of Missouri-Kansas City School of Dentistry 64108.
Rapley
J
J
Cobb
C
C
Spencer
P
P
Killoy
W
W
D000388:Air; D000538:Aluminum Silicates; D002448:Cell Adhesion / Q000528:radiation effects; D002452:Cell Count; D002478:Cells, Cultured; D012534:Dental Scaling; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects*; D006801:Humans; D053685:Laser Therapy; D008855:Microscopy, Electron, Scanning; D008964:Molar, Third; D009354:Neodymium; D010513:Periodontal Ligament / Q000166:cytology / Q000528:radiation effects*; D010865:Pilot Projects; D011208:Powders; D016745:Root Planing; D017550:Spectroscopy, Fourier Transform Infrared; D014092:Tooth Root / Q000166:cytology / Q000528:radiation effects*; D014097:Tooth, Unerupted; D015019:Yttrium
D000538:Aluminum Silicates; D011208:Powders; D009354:Neodymium; D015019:Yttrium