Differential intracellular compartmentalization of phosphotyrosine phosphatases in a glial cell line: TC-PTP versus PTP-1B.
Intracellular levels of protein-phosphotyrosine are regulated by the protein-tyrosine phosphatase (PTP) family. Cellular compartmentalization may play an important role in modulating the function of these enzymes. The recent demonstration that PTP-1B is localized to the endoplasmic reticulum (Frangioni et al: Cell 68:545, 1992) is consistent with this proposition. In this study we have examined the intracellular distribution of TC-PTP in a glial cell line (C6). Using indirect immunofluorescence we have shown that this enzyme is distributed differently from PTP-1B and is mainly concentrated in the perinuclear region of these cells.
Faure
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Département de Médecine, Centre de Recherche du CHUL, St. Foy, Québec, Canada.
Posner
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BI
D000595:Amino Acid Sequence; D000818:Animals; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D005910:Glioma; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009457:Neuroglia / Q000201:enzymology* / Q000648:ultrastructure; D054578:Protein Tyrosine Phosphatase, Non-Receptor Type 2; D017027:Protein Tyrosine Phosphatases / Q000032:analysis*; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D054578:Protein Tyrosine Phosphatase, Non-Receptor Type 2; D017027:Protein Tyrosine Phosphatases; C516653:Ptpn2 protein, mouse