Block in nuclear localization of period protein by a second clock mutation, timeless.
In wild-type Drosophila, the period protein (PER) is found in nuclei of the eyes and brain, and PER immunoreactivity oscillates with a circadian rhythm. The studies described here indicate that the nuclear localization of PER is blocked by timeless (tim), a second chromosome mutation that, like per null mutations, abolishes circadian rhythms. PER fusion proteins without a conserved domain (PAS) and some flanking sequences are nuclear in tim mutants. This suggests that a segment of PER inhibits nuclear localization in tim mutants. The tim gene may have a role in establishing rhythms of PER abundance and nuclear localization in wild-type flies.
Vosshall
L B
LB
Howard Hughes Medical Institute, National Science Foundation Science and Technology Center for Biological Timing, and Laboratory of Genetics, Rockefeller University, New York, NY 10021.
Price
J L
JL
Sehgal
A
A
Saez
L
L
Young
M W
MW
D000818:Animals; D001683:Biological Clocks / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism*; D002940:Circadian Rhythm / Q000235:genetics*; D003593:Cytoplasm / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D017344:Genes, Insect; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D056950:Period Circadian Proteins; D010641:Phenotype; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D029721:Drosophila Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins; D011993:Recombinant Fusion Proteins