An improved method for genotyping of N-acetyltransferase polymorphism by polymerase chain reaction.
Polymorphic N-acetyltransferase in human liver catalyzes N-acetylation of various arylamine-containing drugs and environmental chemicals. To accelerate the pharmacogenetic and ecogenetic studies of N-acetyltransferase polymorphism, we have developed a rapid and simple method for genotyping using a polymerase chain reaction based restriction fragment length polymorphism. This method distinguishes four kinds of allele of the N-acetyltransferase gene using a single polymerase chain reaction starting with a set of primers, followed by successive Asp718, BamHI and TaqI digestions, and then running the samples on a single electrophoresis lane. This method allows us to determine ten different genotypes easily and reliably.
Abe
M
M
Department of Clinical Genetics, Kyushu University, Beppu, Japan.
Suzuki
T
T
Deguchi
T
T
D000483:Alleles; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008099:Liver / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D001191:Arylamine N-Acetyltransferase