title: New markers for linkage analysis of X-linked hypophosphataemic rickets.
abstract: Three polymorphic markers have been used to improve the genetic map of the region Xp22.1-p22.2, which contains the HYP (hypophosphataemic rickets) locus. DXS365 gave no recombinants with HYP, with a peak Lod score of 5.4 at theta = 0.0. A microsatellite marker mPA274 was derived for the DXS274 locus; it detects five alleles with a polymorphism information content of 0.55. Combining information from this microsatellite and the original DXS274 marker, probe CRI-L1391, the peak Lod score for DXS274 against HYP was 9.6 at theta = 0.02. A microsatellite associated with the DXS207 locus (mPA207) gave a peak lod score against HYP of 4.7 at theta = 0.14. A consideration of key recombinants and multilocus analysis suggests the gene order. Xpter-DXS207-DXS43-DXS197-(DXS365,HYP)- DXS274-DXS41-Xcen.
authors.affiliation: Middlesex Hospital, London, UK.
authors.forename: P S
authors.initials: PS
authors.lastname: Rowe
authors.forename: J
authors.initials: J
authors.lastname: Goulding
authors.forename: A
authors.initials: A
authors.lastname: Read
authors.forename: R
authors.initials: R
authors.lastname: Mountford
authors.forename: A
authors.initials: A
authors.lastname: Hanauer
authors.forename: C
authors.initials: C
authors.lastname: Oudet
authors.forename: M P
authors.initials: MP
authors.lastname: Whyte
authors.forename: S
authors.initials: S
authors.lastname: Meier-Ewert
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: K E
authors.initials: KE
authors.lastname: Davies
mesh_terms: D001483:Base Sequence; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D007015:Hypophosphatemia, Familial / Q000235:genetics*; D008126:Lod Score; D008297:Male; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D016324:Sequence Tagged Sites; D014960:X Chromosome
chemical_list: D005819:Genetic Markers