title: Simplified and objective assessment of spermatogenesis in spinal cord injured men by flow cytometry analysis.
abstract: Deterioration of the germinal epithelium of the testis is a known sequela of spinal cord injury (SCI) that may influence the outcome of male reproductive rehabilitation efforts. Quantitative testicular biopsy, currently regarded as the standard of assessing the integrity of spermatogenesis, has not gained wide-spread clinical use because of its invasive nature and relative technical complexity. Alternatively, aspiration DNA flow cytometry analysis of the testis has offered a potential method of spermatogenic assessment that meets both the requirements of simplicity and objectivity. The objective of this study is to determine the capability of flow cytometry to assess spermatogenesis following SCI. Eleven SCI men underwent incisional testicular biopsy with the specimen simultaneously submitted for quantitative evaluation of the germinal epithelium by both quantitative histometry and DNA flow cytometry. The haploid percentage of cells showed highly significant levels of correlation with key micrometric parameters of the quantitative testicular biopsy: spermatid/tubule (p < 0.002) and the spermatid/Sertoli cell ratio (p < 0.0005). Since tissue procurement is accomplished less invasively for flow cytometry analysis, we recommend this method as the modality of assuring integrity of the germinal epithelium in candidates for reproductive rehabilitation.
authors.affiliation: Department of Urology, Jefferson Medical College, Philadelphia, PA 19107.
authors.forename: I H
authors.initials: IH
authors.lastname: Hirsch
authors.forename: D
authors.initials: D
authors.lastname: Kulp-Hugues
authors.forename: J
authors.initials: J
authors.lastname: Sedor
authors.forename: P
authors.initials: P
authors.lastname: McCue
authors.forename: M B
authors.initials: MB
authors.lastname: Chancellor
authors.forename: W E
authors.initials: WE
authors.lastname: Staas
mesh_terms: D000328:Adult; D001706:Biopsy; D004171:Diploidy; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006238:Haploidy; D006801:Humans; D008297:Male; D012016:Reference Values; D012671:Seminiferous Tubules / Q000473:pathology; D013087:Spermatids / Q000473:pathology; D013091:Spermatogenesis; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology*; D013737:Testis / Q000473:pathology