title: Complete nucleotide sequence of the geminivirus tomato yellow leaf curl virus, Thailand isolate.
abstract: The complete genome of a Thailand isolate of the geminivirus tomato yellow leaf curl virus (TYLCV-Th) has been cloned and the nucleotide sequence determined. The genome consists of two DNAs each slightly greater than 2700 nucleotides in length and designated A-DNA and B-DNA. The A-DNA contains six open reading frames (ORFs) capable of encoding proteins with M(r)s greater than 10K; two ORFs were located on the B-DNA. The predicted ORFs were found at positions on the genome similar to those identified in other geminiviruses. Based upon computer-assisted sequence comparisons with other geminiviruses, TYLCV-Th maintained the greatest degree of similarity with a tomato pathogen isolated in Australia, tomato leaf curl virus. Furthermore, sequence comparisons with a large number of geminiviruses confirm further divisions of the geminiviruses based upon geographical proximity of the viruses. Agroinoculation of Nicotiana benthamiana with a TYLCV-Th A-DNA clone containing a mutation in the capsid protein ORF demonstrated that the coat protein is not essential for TYLCV-Th infection but enhances the progression of the disease.
authors.affiliation: Washington University, Department of Biology, St Louis, Missouri 63130.
authors.forename: D E
authors.initials: DE
authors.lastname: Rochester
authors.forename: J J
authors.initials: JJ
authors.lastname: DePaulo
authors.forename: C M
authors.initials: CM
authors.lastname: Fauquet
authors.forename: R N
authors.initials: RN
authors.lastname: Beachy
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D017791:Geminiviridae / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D013785:Thailand
chemical_list: D004279:DNA, Viral