Determination of a novel beta-lactam antibiotic (E-1100) in rat plasma by high-performance liquid chromatography.
A fast, simple and accurate method to determine the concentration of E-1100, a novel beta-lactam antibiotic, in rat plasma has been developed. This method involves deproteinization by methanol with 0.2% perchloric acid and reversed-phase high-performance liquid chromatography with ultraviolet detection. Regression analysis showed that the method was linear over the standard-curve range from 0.191 to 191.0 micrograms/ml. This newly developed method has been applied for the analysis of plasma samples in a preliminary pharmacokinetic study in rats.
Tokumura
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Tsukuba Research Laboratories, Eisai Co., Ibaraki, Japan.
Horie
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D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000493:pharmacokinetics; D002439:Cefotaxime / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D008297:Male; D000432:Methanol / Q000494:pharmacology; D010472:Perchlorates / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis
D000900:Anti-Bacterial Agents; C085927:E 1100; D010472:Perchlorates; D002439:Cefotaxime; D000432:Methanol