title: Structure of pentameric human serum amyloid P component.
abstract: The three-dimensional structure of pentameric human serum amyloid P component at high resolution, the first reported for a pentraxin, reveals that the tertiary fold is remarkably similar to that of the legume lectins. Carboxylate and phosphate compounds bind directly to two calcium ions; interactions with a carboxyethylidene ring are mediated by Asn 59 and Gln 148 ligands of the calcium ions. These X-ray results indicate the probable modes of binding of the biologically important ligands, DNA and amyloid fibrils.
authors.affiliation: Laboratory of Molecular Biology, Birkbeck College, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Emsley
authors.forename: H E
authors.initials: HE
authors.lastname: White
authors.forename: B P
authors.initials: BP
authors.lastname: O'Hara
authors.forename: G
authors.initials: G
authors.lastname: Oliva
authors.forename: N
authors.initials: N
authors.lastname: Srinivasan
authors.forename: I J
authors.initials: IJ
authors.lastname: Tickle
authors.forename: T L
authors.initials: TL
authors.lastname: Blundell
authors.forename: M B
authors.initials: MB
authors.lastname: Pepys
authors.forename: S P
authors.initials: SP
authors.lastname: Wood
mesh_terms: D000595:Amino Acid Sequence; D000686:Amyloidosis / Q000378:metabolism; D001665:Binding Sites; D002097:C-Reactive Protein / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003196:Computer Graphics; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008756:Methylgalactosides / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010767:Phosphorylcholine / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D000683:Serum Amyloid P-Component / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008024:Ligands; D008756:Methylgalactosides; D010714:Phosphatidylethanolamines; D000683:Serum Amyloid P-Component; D010767:Phosphorylcholine; D002097:C-Reactive Protein; D004247:DNA; C042451:methyl 4,6-O-(1-carboxyethylidene)galactopyranoside; D010450:Endopeptidases; D002118:Calcium