[Effects of selenium on experimental ischemic myocardial injuries of heart in organ culture].
The effects of selenium on experimental ischemia, anoxia and ischemia plus anoxia myocardial injuries of hearts in organ culture in vitro were studied. The survival and spontaneous beating of cultured hearts with experimental ischemia, anoxia and ischemia plus anoxia were prolonged by selenium. The lanthanum probe technique and ACPase technique demonstrated that the permeability function of cell membrane, mitochondria membrane, lysosome membrane and Golgi membrane could be protected by selenium. The transition electron microscopy showed that selenium may to some extent maintain membrane system integrity of cultured hearts with experimental ischemia, anoxia and ischemia plus anoxia and postpone the occurrence of irreversible injuries. The obvious difference in amount of intracellular ribosome, polyribosome and short rough surfaced endoplasmic reticulum indicated that selenium may play an important role in synthesis of protein and promoting cell repair.
Zhu
T Y
TY
Department of Pathology, Institute of Basic medical Sciences, Liaoning.
D000818:Animals; D005260:Female; D005318:Fetal Heart / Q000737:chemistry / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D051379:Mice; D008929:Mitochondria, Heart / Q000187:drug effects / Q000648:ultrastructure; D017202:Myocardial Ischemia / Q000473:pathology*; D009206:Myocardium / Q000648:ultrastructure*; D009924:Organ Culture Techniques; D011247:Pregnancy; D012643:Selenium / Q000494:pharmacology*
D012643:Selenium