The growth and behaviour of pig retinal pigment epithelial cells in culture.
The retinal pigment epithelium contains melanin and lipofuscin. It is believed that in the in vivo system, the incomplete degradation of phagocytosed outer segment discs leads to the formation of lipofuscin. Our results showed that pig RPE cells can be successfully cultured using standard culture techniques without addition of specific growth factors. In this system, the autofluorescent material is formed mainly from the degradation of pigment granules. This culture system may provide an excellent model for studying of diseases related to the retina.
Chew
E C
EC
Department of Anatomy, Faculty of Medicine, Chinese University of Hong Kong, Shatin.
Liew
C T
CT
Chew
S B
SB
Lee
J C
JC
Hou
H J
HJ
Yam
H F
HF
Ho
P C
PC
Ip
S M
SM
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure; D008062:Lipofuscin / Q000378:metabolism; D008543:Melanins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000378:metabolism; D013552:Swine
D008062:Lipofuscin; D008543:Melanins