[Production of purified antigenic drug from Mycobacterium tuberculosis humanus and its use for serodiagnosis of tuberculosis].
A variety of antigenic preparations from whole bacteria M. tuberculosis humanus (H37Rv) and relevant subcellular components have been tested using indirect ELISA for determination of antituberculous antibodies. Cell membranes of mycobacteria and antigens isolated from KCl-extracts of cell membranes in gel Toyopearl HW 55 F demonstrated the highest activity. Fraction 6 usage raised the reaction sensitivity to 83.3% in specificity 86.7%. As indicated by the ELISA, the highest antibodies content under the above antigen introduction was reached in patients with fibrocavernous tuberculosis, the lowest one in focal tuberculosis.
Baenskiĭ
A V
AV
Chernousova
L N
LN
Kalinina
O A
OA
Demíanenko
N V
NV
Litvinov
V I
VI
D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D002462:Cell Membrane; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
Poluchenie ochishchennogo antigennogo preparata iz mikobakteriĭ tuberkulëza vida Humanus i ego ispolźovanie dlia serodiagnostiki tuberkulëza.