Expression of rat intestinal L-lysine transport systems in isolated oocytes of Xenopus laevis.
The mechanisms by which cationic amino acids are transported across the intestinal epithelium are poorly understood. We show that isolated stage VI oocytes of Xenopus laevis can express lysine transport activity, which is due to the microinjection of mRNA from rat small intestine. L-Lysine transport activity (0.2 mM, 20 degrees C) reaches 400 pmol.oocyte-1.h-1, compared with a typical endogenous rate of 85 pmol.oocyte-1.h-1. Na(+)-dependence and amino acid inhibition studies resolved the expressed transport activity into three components: 1) a Na(+)-dependent transport system that can be inhibited by leucine with high affinity and also by alanine; 2) a Na(+)-independent system that can be inhibited by leucine with high affinity when Na+ is present, but this affinity is reduced in its absence; 3) a Na(+)-independent system that is inhibited by leucine with high affinity only when Na+ is present. Peak arginine-inhibitable lysine influx was found in a mRNA size fraction of 1.5-2.25 (median 2.0) kb.
Harvey
C M
CM
Department of Physiology, Faculty of Medicine, University of Alberta, Edmonton, Canada.
Muzyka
W R
WR
Yao
S Y
SY
Cheeseman
C I
CI
Young
J D
JD
D000409:Alanine / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002469:Cell Separation; D007267:Injections; D007413:Intestinal Mucosa / Q000378:metabolism*; D007930:Leucine / Q000494:pharmacology; D008239:Lysine / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics*; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000494:pharmacology; D012333:RNA, Messenger / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014982:Xenopus laevis
D012333:RNA, Messenger; D011061:Poly A; D007930:Leucine; D008239:Lysine; D000409:Alanine