Human immunodeficiency virus type 1 Nef associates with a cellular serine kinase in T lymphocytes.
With T-cell lines constitutively expressing Nef from the SF2 strain of human immunodeficiency virus type 1 (HIV-1SF2) in the form of a hybrid CD8-Nef fusion protein or T-cell lines chronically infected with HIV-1SF2, a cellular serine kinase was found that specifically associates with Nef. Proteins of 62 kDa and 72 kDa, which coimmunoprecipitated with Nef, were phosphorylated in in vitro kinase assays. This Nef-associated serine kinase activity was not blocked by inhibitors of protein kinase C or protein kinase A and was lost when Nef was truncated at amino acid 94 or 99. These findings present evidence that a serine kinase activity is associated with Nef expressed in human T lymphocytes.
Sawai
E T
ET
Department of Medicine, University of California, San Francisco 94143-0128.
Baur
A
A
Struble
H
H
Peterlin
B M
BM
Levy
J A
JA
Cheng-Mayer
C
C
D016827:CD8 Antigens / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D004252:DNA Mutational Analysis; D015693:Gene Products, nef / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D046911:Macromolecular Substances; D010768:Phosphoserine / Q000032:analysis; D011233:Precipitin Tests; D011485:Protein Binding; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017384:Sequence Deletion; D013601:T-Lymphocytes / Q000382:microbiology*; D054311:nef Gene Products, Human Immunodeficiency Virus; D054462:p21-Activated Kinases
D016827:CD8 Antigens; D015693:Gene Products, nef; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus; D010768:Phosphoserine; D017346:Protein Serine-Threonine Kinases; D054462:p21-Activated Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C