title: Inter-institutional reproducibility of flow cytometric DNA-analysis in breast carcinomas.
abstract: In order to study interinstitutional reproducibility of flow cytometric DNA-analysis (DNA-FCM), frozen pieces from 30 consecutive breast carcinomas were analysed by 5 laboratories. Different instruments, preparation and DNA staining methods were used. A concordance in DNA-ploidy status was obtained in 26 of the 30 tumours. The discrepancy can mainly be explained by intratumoural DNA-heterogeneity since a complete agreement in ploidy status was obtained when four of the laboratories analysed the same cell suspension, where solid bits showed differing results. The sampling method seems therefore to be a crucial step for the results and needs further studies. As far as the estimation of S-phase fraction was concerned, one laboratory obtained significantly higher values compared to the other four. The correlation between the other four laboratories varied between r = 0.66-0.92.
authors.affiliation: Department of Pathology, Medical Center Hospital, Orebro, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Risberg
authors.forename: B
authors.initials: B
authors.lastname: Baldetorp
authors.forename: M
authors.initials: M
authors.lastname: Fernö
authors.forename: B
authors.initials: B
authors.lastname: Hansson
authors.forename: L L
authors.initials: LL
authors.lastname: Mossberg
authors.forename: G
authors.initials: G
authors.lastname: Roos
authors.forename: O
authors.initials: O
authors.lastname: Stål
authors.forename: J T
authors.initials: JT
authors.lastname: Thornthwaite
authors.forename: L
authors.initials: L
authors.lastname: Tibell
mesh_terms: D000047:Academies and Institutes / Q000592:standards; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D011003:Ploidies; D012044:Regression Analysis; D015203:Reproducibility of Results; D016196:S Phase
chemical_list: D004273:DNA, Neoplasm