title: Cytotoxicity versus antibacterial activity of some antiseptics in vitro.
abstract: Through the development of new techniques for bacterial growth and isolation, obligate anaerobes have been shown to be more prevalent than previously thought in the pathogenesis of periapical and pulpal diseases. A follow-up study was conducted to examine the germicidal effect of 0.05% NaOCl, 10 mg/ml metronidazole and supernatant of Ca (OH)2 (0.025 ml) on four anaerobic microorganisms commonly found in root canals, and to compare the toxicity of these substances on cell cultures. In vitro testing revealed that 0.05% NaOCl and Ca (OH)2 were both equally effective on these anaerobes. Also, metronidazole was found to be germicidally effective against Bacteroides melaninogenicus, Bacteroides oralis and Peptostreptococcus anaerobius, but ineffective against Veillonella alcalescens. Furthermore, it was found that NaOCl and Ca (OH)2 had a very destructive effect on cell cultures compared with their antimicrobial effect, whereas metronidazole was less toxic among the agents tested.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Gazi University, Ankara, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Alaçam
authors.forename: H
authors.initials: H
authors.lastname: Omürlü
authors.forename: A
authors.initials: A
authors.lastname: Ozkul
authors.forename: G
authors.initials: G
authors.lastname: Görgül
authors.forename: A
authors.initials: A
authors.lastname: Misirligil
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D001439:Bacteroides / Q000187:drug effects; D002126:Calcium Hydroxide / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology* / Q000633:toxicity; D010462:Peptostreptococcus / Q000187:drug effects; D001443:Prevotella melaninogenica / Q000187:drug effects; D012973:Sodium Hypochlorite / Q000494:pharmacology* / Q000633:toxicity; D014407:Tumor Cells, Cultured; D014678:Veillonella / Q000187:drug effects
chemical_list: D008795:Metronidazole; D012973:Sodium Hypochlorite; D002126:Calcium Hydroxide