title: Chiral differentiation of the optical isomers of racemethorphan and racemorphan in urine by capillary zone electrophoresis.
abstract: A novel capillary zone electrophoretic method based on the use of propanol-modified beta-cyclodextrin buffers has been developed; it unambiguously detects and quantitates the optical isomers of racemethorphan and racemorphan without prior derivatization. Detection limits in urine down to 20 ppb have been obtained. The method uses a solid-phase extraction recovery of analytes followed by ultraviolet detection. Separation conditions include the use of 60mM beta-cyclodextrin and 50mM sodium dodecyl sulfate in a buffer containing 20% 1-propanol.
authors.affiliation: Australian Government Analytical Laboratories, Pymble, NSW.
authors.forename: A
authors.initials: A
authors.lastname: Aumatell
authors.forename: R J
authors.initials: RJ
authors.lastname: Wells
mesh_terms: D003915:Dextromethorphan / Q000652:urine*; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D007981:Levorphanol / Q000652:urine*; D008297:Male; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D007981:Levorphanol; D003915:Dextromethorphan