title: Is buffering capacity the principle role of the jelly coat in Bufo arenarum fertilization?
abstract: 1. Dejellied Bufo arenarum oocytes can be fertilized in Ringer-Phosphate buffer with the same efficiency as jellied (control) oocytes. 2. Ringer-Phosphate buffer at pH = 7.4 not only provides buffering capacity but also the delta pH necessary for the acrosomal reaction. 3. The use of Ringer-TRIS buffer at pH = 7.4 does not render as good as Ringer-Phosphate buffer results, in terms of fertilization percentages. 4. Insemination of oocytes in Ringer-TRIS buffer interferes with early development.
authors.affiliation: LIBIQUIMA, Departamento de Química, Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires, Argentina.
authors.forename: T M
authors.initials: TM
authors.lastname: Fonovich de Schroeder
authors.forename: L
authors.initials: L
authors.lastname: Gauna
authors.forename: A M
authors.initials: AM
authors.lastname: Pechén de D'Angelo
mesh_terms: D000818:Animals; D002022:Bufo arenarum; D005260:Female; D005306:Fertilization / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000502:physiology*