title: Analysis of the locus D1S80 by amplified fragment length polymorphism technique (AMP-FLP). Frequency distribution in Danes. Intra and inter laboratory reproducibility of the technique.
abstract: DNA from the locus D1S80 was amplified by polymerase chain reaction (PCR) and analyzed by electrophoresis in vertical polyacrylamide gels followed by silverstaining. DNA samples from 119 unrelated Danes and 97 mother/child pairs were examined. The amplified fragment length polymorphism (AMP-FLP) analysis of the D1S80 locus demonstrated 21 alleles and a heterozygosity of 77%. Of the 231 possible phenotypes, 57 were observed. All mother/child pairs shared at least one D1S80 allele. The D1S80 typing results in 70 Danes were compared to the results obtained on the same samples in another laboratory and the results were concordant in all cases.
authors.affiliation: Institute of Forensic Genetics, University of Copenhagen, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Thymann
authors.forename: L J
authors.initials: LJ
authors.lastname: Nellemann
authors.forename: G
authors.initials: G
authors.lastname: Masumba
authors.forename: L
authors.initials: L
authors.lastname: Irgens-Møller
authors.forename: N
authors.initials: N
authors.lastname: Morling
mesh_terms: D000328:Adult; D000483:Alleles; D002648:Child; D004247:DNA / Q000032:analysis*; D003718:Denmark; D005260:Female; D006801:Humans; D008297:Male; D009035:Mothers; D010334:Paternity; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D044465:White People / Q000235:genetics*
chemical_list: D004247:DNA