title: Human immunodeficiency virus type 1 Nef associates with a cellular serine kinase in T lymphocytes.
abstract: With T-cell lines constitutively expressing Nef from the SF2 strain of human immunodeficiency virus type 1 (HIV-1SF2) in the form of a hybrid CD8-Nef fusion protein or T-cell lines chronically infected with HIV-1SF2, a cellular serine kinase was found that specifically associates with Nef. Proteins of 62 kDa and 72 kDa, which coimmunoprecipitated with Nef, were phosphorylated in in vitro kinase assays. This Nef-associated serine kinase activity was not blocked by inhibitors of protein kinase C or protein kinase A and was lost when Nef was truncated at amino acid 94 or 99. These findings present evidence that a serine kinase activity is associated with Nef expressed in human T lymphocytes.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143-0128.
authors.forename: E T
authors.initials: ET
authors.lastname: Sawai
authors.forename: A
authors.initials: A
authors.lastname: Baur
authors.forename: H
authors.initials: H
authors.lastname: Struble
authors.forename: B M
authors.initials: BM
authors.lastname: Peterlin
authors.forename: J A
authors.initials: JA
authors.lastname: Levy
authors.forename: C
authors.initials: C
authors.lastname: Cheng-Mayer
mesh_terms: D016827:CD8 Antigens / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D004252:DNA Mutational Analysis; D015693:Gene Products, nef / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D046911:Macromolecular Substances; D010768:Phosphoserine / Q000032:analysis; D011233:Precipitin Tests; D011485:Protein Binding; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017384:Sequence Deletion; D013601:T-Lymphocytes / Q000382:microbiology*; D054311:nef Gene Products, Human Immunodeficiency Virus; D054462:p21-Activated Kinases
chemical_list: D016827:CD8 Antigens; D015693:Gene Products, nef; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus; D010768:Phosphoserine; D017346:Protein Serine-Threonine Kinases; D054462:p21-Activated Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C