Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain.
Sequencing and computer analysis of the nucleotide sequence of the variola virus strain India-1967 (VAR) genome segment (43069 bp) from the region of HindIII C, E, R, Q, K, H DNA fragments has been carried out. Forty-three potential open reading frames (ORFs) have been identified, and the polypeptides encoded by them have been compared with the analogous proteins of vaccinia virus strain Copenhagen (COP). ORF E7R of VAR is much shorter than the COP analog. The other polypeptides coded by the potential ORFs of VAR are highly conserved in comparison with COP. Possible functions of the predicted viral polypeptides are discussed.
Shchelkunov
S N
SN
Institute of Molecular Biology, NPO Vector, Koltsovo, Novosibirsk Region, Russia.
Blinov
V M
VM
Resenchuk
S M
SM
Totmenin
A V
AV
Sandakhchiev
L S
LS
D000358:African Swine Fever Virus / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011506:Proteins / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014616:Vaccinia virus / Q000235:genetics; D012901:Variola virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D005656:Fungal Proteins; D011506:Proteins; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase