Endogenous hepoxilin A3, produced under short duration of high shear-stress, inhibits thrombin-induced aggregation in human platelets.
The effect of short duration of shear-stress (350 dyne/cm2, 20 ms) on platelet-aggregation has been assessed. This treatment inhibits thrombin-induced but not ADP- or collagen-induced aggregation. The inhibitory effect is mediated by endogenous hepoxilin A3. This conclusion is based on the following observations: (a) The shear-stress effect is abolished by lipoxygenase inhibitors. (b) Hepoxilin A3 mimics the shear-stress effect.
Margalit
A
A
Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Granot
Y
Y
D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013917:Thrombin / Q000037:antagonists & inhibitors*; D013997:Time Factors
D016859:Lipoxygenase Inhibitors; D010975:Platelet Aggregation Inhibitors; C038243:8-hydroxy-11,12-epoxyeicosa-5,9,14-trienoic acid; D013917:Thrombin; D015126:8,11,14-Eicosatrienoic Acid