title: Cell adhesion molecule expression in the regenerating rat facial nucleus.
abstract: Transection of the rat facial nerve leads to an intrinsic microglial reaction in the facial nucleus. In the present study, we have examined immunocytochemically the expression of cell adhesion molecules during this process. Resting microglia constitutively expressed the LFA-1 alpha and beta chain (CD11a and CD18) in the white matter, rather than in the intact, control facial nucleus. From 24 h after facial nerve transection onward, activated microglia showed an increased expression of LFA-1 alpha and beta. The immunoreactivity reached its peak around day 7 following axotomy, i.e. at a time when activated microglia are found in a close perineuronal position. In contrast, the expression of the principal ligand of LFA-1, i.e. ICAM-1, remained unchanged following axotomy; the immunoreactivity being constitutively found on cerebral blood vessels. Increased LFA-1 expression thus appears to be a general marker for microglial activation and might further be involved in the rearrangement of the microglial cytoskeleton upon activation of this cell.
authors.affiliation: Max-Planck-Institute of Psychiatry, Department of Neuromorphology, Martinsried, Germany.
authors.forename: M E
authors.initials: ME
authors.lastname: Moneta
authors.forename: J
authors.initials: J
authors.lastname: Gehrmann
authors.forename: R
authors.initials: R
authors.lastname: Töpper
authors.forename: R B
authors.initials: RB
authors.lastname: Banati
authors.forename: G W
authors.initials: GW
authors.lastname: Kreutzberg
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003714:Denervation; D005154:Facial Nerve / Q000502:physiology*; D018799:Intercellular Adhesion Molecule-1; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D009416:Nerve Regeneration; D011149:Pons / Q000378:metabolism* / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D016028:Receptors, Leukocyte-Adhesion / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D016028:Receptors, Leukocyte-Adhesion; D018799:Intercellular Adhesion Molecule-1