Hepatic oxygen consumption and cytochrome P450 activity in experimental faecal peritonitis.
OBJECTIVE
To study hepatic oxygen consumption and cytochrome P450 activity in pigs with septic shock induced by faecal peritonitis.


METHODS
Controlled experimental study.


METHODS
12 pigs weighing 19-27 kg.


METHODS
The animals were divided into a control group (n = 6) and a peritonitis group (n = 6). Peritonitis was induced by intraperitoneal instillation of a standard amount of autologous faeces. The animals were then observed for 300 minutes. Liver biopsy specimens were taken at 0 and 300 minutes.


METHODS
Hepatic oxygen delivery (DO2) and consumption (VO2). Cytochrome P450 activity was studied by measuring O- and N-demethylation of codeine at 0 and 300 minutes.


RESULTS
Hepatic DO2 was reduced, whereas VO2 was increased during sepsis. There were no significant changes in the N- and O-demethylation of codeine.


CONCLUSIONS
Hepatic VO2 did increase during sepsis, possibly because of the increased metabolic demand. Cytochrome P450 activity was unaffected by the septic challenge.
Rasmussen
I
I
Department of Surgery, University Hospital, Uppsala, Sweden.
Rane
A
A
Haglund
U
U
D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure; D001810:Blood Volume; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005243:Feces; D005260:Female; D006503:Hepatic Veins / Q000378:metabolism / Q000503:physiopathology; D008099:Liver / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D010101:Oxygen Consumption; D010538:Peritonitis / Q000378:metabolism* / Q000503:physiopathology; D011169:Portal Vein / Q000378:metabolism / Q000503:physiopathology; D011651:Pulmonary Artery / Q000378:metabolism / Q000503:physiopathology; D011897:Random Allocation; D013552:Swine; D014655:Vascular Resistance
D003577:Cytochrome P-450 Enzyme System