Direct enantioselective separation of bevantolol by high-performance liquid chromatography on normal and reverse cellulose chiral stationary phases.
A direct, isocratic and simple chromatographic method is described for the enantiomeric separation of bevantolol (BEV) using normal and reverse cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary phases (CSPs) known as Chiralcel OD and Chiralcel OD-R, respectively. The effect of various alcohols present in the mobile phase on the separation factor (a) and resolution factor (Rs) has been studied. The method has been used to determine and identify the BEV enantiomers in human urine after oral administration of racemic BEV. The chiral recognition mechanism(s) between the analyte and these chiral stationary phases is proposed.
Aboul-Enein
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HY
Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia.
Serignese
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D002482:Cellulose; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D011412:Propanolamines / Q000652:urine*; D013237:Stereoisomerism
D011412:Propanolamines; C021148:bevantolol; D002482:Cellulose