Involvement of eicosanoids and inositol 1,4,5-trisphosphate in serotonin-induced secretion in rat ileum in vivo.
1. The possible involvement of eicosanoids, cyclic adenosine monophosphate (c-AMP), and inositol 1,4,5-trisphosphate (IP3) in serotonin-(5-hydroxytryptamine-) induced secretion in rat ileum in vivo was studied. 2. Serotonin caused a significant increase in mean IP3 levels. Indomethacin reduced serotonin-induced secretion of water by 28%. Thus, IP3 and eicosanoids appear to be intracellular mediators of serotonin-induced secretion in rat ileum. 3. Serotonin, BRL 24924, and cisapride all inhibited theophylline-induced increases in c-AMP concentrations in rat ileal segments. Neither BRL 24924 nor cisapride caused any change in IP3 levels. 4. These data suggest that a 5-HT2 receptor dependent on IP3 and eicosanoids is involved in mediating in serotonin-induced secretion in rat small intestine.
Hansen
M B
MB
Department of Surgery, State University of New York, Health Science Center, Brooklyn 11203.
Jaffe
B M
BM
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D015777:Eicosanoids / Q000502:physiology*; D004573:Electrolytes / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism / Q000502:physiology*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000494:pharmacology*; D014867:Water / Q000378:metabolism
D015777:Eicosanoids; D004573:Electrolytes; D014867:Water; D012701:Serotonin; D015544:Inositol 1,4,5-Trisphosphate; D000242:Cyclic AMP; D007213:Indomethacin