Enterostatin--its ability to inhibit insulin secretion and to decrease high-fat food intake.
Enterostatin is a peptide which has been found to decrease food intake with a specificity for the fat contained in the food. In this work we have investigated the effect of enterostatin (Val-Pro-Asp-Pro-Arg) and its proteolytic fragments, des-arg-enterostatin (Val-Pro-Asp-Pro) and the tripeptide Asp-Pro-Arg, on insulin secretion. It was found that enterostatin and desarg-enterostatin inhibited insulin secretion from isolated rat islets by 55.3% (P < 0.05) and 53.6% (P < 0.05) at 1.6 x 10(-4) M concentration, while the tripeptide Asp-Pro-Arg at 1.6 x 10(-4) M concentration had no significant effect and increased insulin secretion by 33.0%. Enterostatin at 200 ng after intraventricular administration was found to inhibit the intake of a high-fat diet by 45.0%, while des-arg-enterostatin (200 ng) had no effect, in agreement with previous findings. The tripeptide Asp-Pro-Arg (200 ng) had no effect on the intake of a high-fat diet compared to saline injection. The ability of enterostatin to inhibit high-fat food intake and decrease insulin secretion may be important for the prevention of obesity and type II diabetes, conditions linked through hyperinsulinemia.
Mei
J
J
Department of Medical and Physiological Chemistry, University of Lund, Sweden.
Cheng
Y
Y
Erlanson-Albertsson
C
C
D000595:Amino Acid Sequence; D000818:Animals; D003089:Colipases / Q000008:administration & dosage / Q000494:pharmacology*; D004041:Dietary Fats / Q000378:metabolism*; D004792:Enzyme Precursors; D005247:Feeding Behavior / Q000187:drug effects*; D005260:Female; D066298:In Vitro Techniques; D007276:Injections, Intraventricular; D007328:Insulin / Q000378:metabolism*; D007329:Insulin Antagonists / Q000494:pharmacology; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D003089:Colipases; D004041:Dietary Fats; D004792:Enzyme Precursors; D007328:Insulin; D007329:Insulin Antagonists; D011498:Protein Precursors; C037107:procolipase