CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay.
We evaluated a flow cytometric method for determining the proportion of CD4-positive T lymphocytes in whole blood using a single three-color tube containing fluorochrome-labeled CD45, CD3, and CD4. Various ways of gating this sample were evaluated and results were compared with data obtained in our standard six-tube, two-color assay gated on light scatter parameters. Excellent correlation was found between the three-color analysis using a CD45/side scatter gate and the standard two-color analysis with a light scatter gate. This single-tube three-color test is a promising assay for monitoring CD4+ T-lymphocytes.
Nicholson
J K
JK
Immunology Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Jones
B M
BM
Hubbard
M
M
D000328:Adult; D001773:Blood Cells / Q000166:cytology* / Q000276:immunology; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002648:Child; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D006679:HIV Seropositivity; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D011786:Quality Control
D017252:CD3 Complex; D015704:CD4 Antigens; D005456:Fluorescent Dyes; D017493:Leukocyte Common Antigens