Role of polymorphonuclear leucocytes and oxygen-derived free radicals in the formation of gastric lesions induced by HCl/ethanol, and a possible mechanism of protection by anti-ulcer polysaccharide.
This study examined the role of oxygen-derived free radicals in the pathogenesis of gastric mucosal lesions induced by HCl/ethanol. Superoxide dismutase, and catalase, and their combination reduced gastric lesion formation in mice. Gastric lesions were also reduced in mice treated with cyclophosphamide or anti-neutrophils, but not in mice treated with allopurinol or desulphated-carrageenan. Cobra venom factor did not reduce lesion formation. These results suggested that oxygen-free radicals may contribute to the formation of gastric mucosal lesions induced by HCl/ethanol, and that oxygen radicals were generated from neutrophils but not from xanthine oxidase. Anti-ulcer pectic polysaccharide, bupleuran 2IIc, which was recently isolated from the roots of Bupleurum falcatum L., showed potent inhibition of HCl/ethanol-induced gastric lesions in mice. Bupleuran 2IIc seemed to scavenge hydroxyl radical effectively. It was suggested that this anti-ulcer polysaccharide may provide protection to the gastric mucosa by scavenging oxygen-free radicals.
Matsumoto
T
T
Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
Moriguchi
R
R
Yamada
H
H
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D002351:Carrageenan / Q000494:pharmacology; D002417:Cattle; D003520:Cyclophosphamide / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism / Q000633:toxicity*; D016166:Free Radical Scavengers; D005753:Gastric Mucosa / Q000187:drug effects; D006851:Hydrochloric Acid / Q000378:metabolism / Q000633:toxicity*; D006878:Hydroxides / Q000378:metabolism / Q000633:toxicity; D017665:Hydroxyl Radical; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009504:Neutrophils / Q000502:physiology*; D010368:Pectins / Q000627:therapeutic use*; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000633:toxicity*; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000517:prevention & control*; D013481:Superoxides / Q000378:metabolism / Q000633:toxicity
D000897:Anti-Ulcer Agents; D016166:Free Radical Scavengers; D006878:Hydroxides; D017382:Reactive Oxygen Species; C082983:desulfated carrageenan; D013481:Superoxides; C073829:Bupleuran 2IIc; D017665:Hydroxyl Radical; D000431:Ethanol; D000493:Allopurinol; D010368:Pectins; D003520:Cyclophosphamide; D002351:Carrageenan; D006851:Hydrochloric Acid