title: Magnetization transfer affects the proton creatine/phosphocreatine signal intensity: in vivo demonstration in the rat brain.
abstract: The effect of off-resonance preirradiation on proton spectra acquired from the healthy rat brain at 4.7 T is examined using a PRESS sequence. The creatine/phosphocreatine (Cr/PCr) signal at 3.0 ppm decreases in signal intensity for offset frequencies between +/- 10 kHz. This cannot be explained by RF bleedover, but is attributed to magnetization transfer between two pools of Cr/PCr, one with a long T2 relaxation time giving the observed NMR signal, the other corresponding to a broad resonance line being completely or partially saturated by off-resonance preirradiation. Possible interpretations of these results are discussed.
authors.affiliation: Universität Bremen, Fachbereich Chemie/Biologie, Bremen, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Dreher
authors.forename: D G
authors.initials: DG
authors.lastname: Norris
authors.forename: D
authors.initials: D
authors.lastname: Leibfritz
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000032:analysis; D001923:Brain Chemistry; D003401:Creatine / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D010725:Phosphocreatine / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010725:Phosphocreatine; D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine