title: Protein EnvM is the NADH-dependent enoyl-ACP reductase (FabI) of Escherichia coli.
abstract: The EnvM protein was purified from an overproducing Escherichia coli strain. It showed NADH-dependent enoyl-acyl carrier protein (ACP) reductase activity using both crotonyl-ACP and crotonyl-CoA as substrates. The protein bound a radioactive diazaborine derivative in the presence of NAD+ and radioactive NAD+ in the presence of the drug. Based on these data, it is concluded that EnvM is the NADH-dependent enoyl-ACP reductase (EC 1.3.1.9) of E. coli and we propose to rename the corresponding gene fabI.
authors.affiliation: Institut für Mikrobiologie, Universität Graz, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Bergler
authors.forename: P
authors.initials: P
authors.lastname: Wallner
authors.forename: A
authors.initials: A
authors.lastname: Ebeling
authors.forename: B
authors.initials: B
authors.lastname: Leitinger
authors.forename: S
authors.initials: S
authors.lastname: Fuchsbichler
authors.forename: H
authors.initials: H
authors.lastname: Aschauer
authors.forename: G
authors.initials: G
authors.lastname: Kollenz
authors.forename: G
authors.initials: G
authors.lastname: Högenauer
authors.forename: F
authors.initials: F
authors.lastname: Turnowsky
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D050753:Enoyl-(Acyl-Carrier-Protein) Reductase (NADH); D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D054889:Fatty Acid Synthase, Type II; D007700:Kinetics; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D004364:Pharmaceutical Preparations; D009243:NAD; C010701:crotonyl-coenzyme A; D010088:Oxidoreductases; D050753:Enoyl-(Acyl-Carrier-Protein) Reductase (NADH); C493924:fabI protein, E coli; D054889:Fatty Acid Synthase, Type II