Molecular cloning and functional expression of a human peroxisomal acyl-coenzyme A oxidase.
cDNA encoding the human peroxisomal acyl-coenzyme A oxidase (AOX) was cloned and sequenced. The longest cDNA insert isolated has 3083 bases and encodes the entire protein of 661-amino acids, including the carboxyl-terminal sequence (Ser-Lys-Leu) known as a minimal peroxisome-targeting signal. At the amino acid level, the significantly high homology (89%) to rat AOX was found. In the cDNA-expression experiment, significant amount of AOX was accumulated in human skin fibroblast and the expressed AOX was catalytically active, while only a limited amount was found in Zellweger syndrome patient's fibroblast not having normal peroxisomes.
Aoyama
T
T
Department of Biochemistry, Shinshu University School of Medicine, Nagano, Japan.
Tsushima
K
K
Souri
M
M
Kamijo
T
T
Suzuki
Y
Y
Shimozawa
N
N
Orii
T
T
Hashimoto
T
T
D042962:Acyl-CoA Oxidase; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D005347:Fibroblasts / Q000201:enzymology; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D012016:Reference Values; D012867:Skin / Q000201:enzymology*; D014616:Vaccinia virus; D015211:Zellweger Syndrome / Q000201:enzymology
D017931:DNA Primers; D012333:RNA, Messenger; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase