Characterization of [35S]lanthionine ketimine specific binding to bovine brain membranes.
[35S]Lanthionine ketimine binds specifically and with high affinity to bovine brain membranes. This binding has been studied in detail. It is reversible, not occurring at an uptake site or at a metabolizing enzyme and depending only weakly from ionic strength; it is affected by thiol reagents. [35S]Lanthionine ketimine specific binding is displaced only by other ketimines and by catecholamines, but not by more selective adrenergic ligands; binding parameters are reported. [3H]Adrenaline but not [3H]dihydroalprenolol is partially displaced by lanthionine ketimine. With bovine brain preparations a significant stimulation of basal adenylate cyclase activity by lanthionine ketimine is observed.
Duprè
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Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Università La Sapienza, Roma, Italy.
Fontana
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Costa
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Pecci
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Ricci
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Cavallini
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D000262:Adenylyl Cyclases / Q000378:metabolism*; D000603:Amino Acids, Sulfur / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism; D007700:Kinetics; D018377:Neurotransmitter Agents / Q000494:pharmacology; D013462:Sulfur Radioisotopes; D014422:Turkeys
D000603:Amino Acids, Sulfur; D018377:Neurotransmitter Agents; D013462:Sulfur Radioisotopes; C035499:lanthionine ketimine; D000262:Adenylyl Cyclases