title: Effect of moderate exercise on proliferative responses of peripheral blood mononuclear cells.
abstract: We studied the effects of 30 minutes of exercise on T lymphocyte counts and proliferative responses of peripheral blood mononuclear cells (PBMC) in 25 runners. Exercise resulted in a T lymphocytosis in the immediate post-exercise period in all subjects (p < 0.001), and reduced CD4+/CD8+ ratios in 22/25 subjects (p = 0.001). The change was due primarily to a 2.2-fold increase in CD8+ cells (p < 0.001). Exercise also reduced PBMC mitogenic responses to phytohemagglutinin (PHA) in 13/14 subjects (p = 0.049), and to pokeweed mitogen (PWM) in 11/14 subjects (p = 0.022), but not to concanavalin A. Postrun sera from 5 of 6 subjects inhibited PHA but not PWM responses of resting autologous PBMC with normal CD4+/CD8+ ratios (p < or = 0.05): indomethacin and monocyte depletion blocked the serum inhibition (p = 0.003, p = 0.0006, respectively). We conclude that post-exercise suppression of mitogenic responses to PHA is due to the release of a serum factor(s) capable of inducing prostaglandin synthesis by circulating monocytes, whereas exercise-induced suppression of PWM responses depends primarily on the reversal of CD4+/CD8+ ratios.
authors.affiliation: Department of Medicine, James H. Quillen College of Medicine, East Tennessee State University, Johnson City.
authors.forename: J
authors.initials: J
authors.lastname: Smith
authors.forename: D
authors.initials: D
authors.lastname: Chi
authors.forename: S
authors.initials: S
authors.lastname: Salazar
authors.forename: G
authors.initials: G
authors.lastname: Krish
authors.forename: S
authors.initials: S
authors.lastname: Berk
authors.forename: S
authors.initials: S
authors.lastname: Reynolds
authors.forename: G
authors.initials: G
authors.lastname: Cambron
mesh_terms: D000328:Adult; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology; D003208:Concanavalin A / Q000494:pharmacology; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology*; D008213:Lymphocyte Activation / Q000502:physiology; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000502:physiology
chemical_list: D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A; D007213:Indomethacin