HLA DQ and DP locus mismatches and their effect on MLC in HLA class I- and DRB1-matched unrelated patient/donor pairs waiting for allogeneic bone-marrow transplantation.
The frequency of HLA DQA1, DQB1, and DPB1 mismatches was evaluated in 110 HLA class I- and DRB1-matched, unrelated pairs who were tested in mixed lymphocyte culture (MLC) in connection with a potential allogeneic bone-marrow transplantation between unrelated persons. After typing of the DQA1, DQB1, and DPB1 genes retrospectively, it was found that only 22% of the pairs were matched for all the loci tested. However, the frequency of this fully matched group was higher (37%) in pairs where both individuals were of Finnish origin as compared to those where the donor originated from a foreign registry. This suggests that the variety of haplotypes may be limited in small populations and justifies local national registries. The overall correlation between class-II match and stimulation in MLC was clear: better matches gave lower responses. However, the correlation was not definitive; for example, no response was observed in some cases with mismatches only in DPB1.
Partanen
J
J
Finnish Red Cross Blood Transfusion Service, Tissue Typing Laboratory, Helsinki.
Koskimies
S
S
D000483:Alleles; D016026:Bone Marrow Transplantation / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology; D015235:HLA-B Antigens / Q000276:immunology; D006683:HLA-DQ Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D014019:Tissue Donors
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007135:Immunoglobulin Variable Region