title: The determination of biogenic amines in four strains of the fruit fly Drosophila melanogaster.
abstract: A range of biogenic amines were measured in the heads from four strains of Drosophila melanogaster. Quantitation was carried out using gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) with stable isotope dilution. The principal amines detected in the heads were dopamine, noradrenaline and 5 HT with small amounts of p- and m-tyramine; p-octopamine could not be detected in samples of 25 heads with a limit of detection of 10 pg per sample. In addition to conventional neurotransmitters or putative neurotransmitters the amines 5- and 6-hydroxydopamine were detected in the heads in substantial amounts.
authors.affiliation: Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
authors.forename: D G
authors.initials: DG
authors.lastname: Watson
authors.forename: P
authors.initials: P
authors.lastname: Zhou
authors.forename: J M
authors.initials: JM
authors.lastname: Midgley
authors.forename: C D
authors.initials: CD
authors.lastname: Milligan
authors.forename: K
authors.initials: K
authors.lastname: Kaiser
mesh_terms: D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis*; D004298:Dopamine / Q000032:analysis; D004331:Drosophila melanogaster / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006257:Head; D006892:Hydroxydopamines / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis; D009655:Octopamine / Q000032:analysis; D012701:Serotonin / Q000032:analysis; D014439:Tyramine / Q000032:analysis
chemical_list: D015306:Biogenic Monoamines; D006892:Hydroxydopamines; D009655:Octopamine; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine; D014439:Tyramine