[Densitometric determination of dithiadenoxide in blood].
The present paper describes a method of dithiadenoxide determination in blood plasma. Dithiadenoxide was reduced to dithiaden with zinc dust in acidified plasma. The solution was alkalified and dithiaden extracted into n-hexane and isolated on a thin layer of silica gel using the system chloroform--n-heptane-methanol (60:22:18). After spraying the plate with a solution of m-nitrophenyldiazoniumfluoroborate in acid medium, dithiaden stains were evaluated densitometrically in the absorption maximum of the reaction product at 565 nm (RF = 0.37). Detection limit of dithiadenoxide in the proposed method is 0.004 microgram in an applied amount.
Peterková
M
M
Výzkumný ústav pro farmacii a biochemii.
Matousová
O
O
D001582:Benzothiepins / Q000097:blood*; D002845:Chromatography / Q000379:methods*; D006634:Histamine H1 Antagonists / Q000097:blood; D006801:Humans
D001582:Benzothiepins; D006634:Histamine H1 Antagonists; C083350:dithiadenoxide; C100170:dithiadene
Densitometrické stanovení dithiadenoxidu v krevní plazmĕ.