title: A comparison of the yields of translocations and dicentrics measured using fluorescence in situ hybridization.
abstract: Chromosome aberration analysis using fluorescence in situ hybridization (FISH) methods without centromere-specific markers results in the misscoring of a substantial fraction of the dicentrics as translocations; that is, too many translocations and too few dicentrics are scored. Such misscoring has led to considerable confusion in the rapidly emerging literature on FISH-based cytogenetics. Here, we demonstrate that the problem is fully resolved when centromeric probes are used.
authors.affiliation: University of California, Lawrence Livermore National Laboratory, Livermore, CA 94551.
authors.forename: T
authors.initials: T
authors.lastname: Straume
authors.forename: J N
authors.initials: JN
authors.lastname: Lucas
mesh_terms: D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000528:radiation effects*; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D014178:Translocation, Genetic
chemical_list: D015342:DNA Probes