title: A peptide-model for the heparin-binding property of pseudorabies virus glycoprotein III.
abstract: The pseudorabies virus glycoprotein III (PrV-gIII) has been identified previously as the major viral component binding to a heparin-like receptor on the surface of target cells. The amino acid sequence of gIII contains three regions corresponding to consensus sequences for heparin binding. A synthetic peptide corresponding to amino acids 134 to 141 of PrV-gIII bound heparin in a dot blot assay. In contrast, a synthetic peptide derived from amino acids 290-299 of PrV-gIII did not bind heparin. We therefore conclude that the region containing amino acid 134-141 is involved in binding to the heparin-like cellular receptor.
authors.affiliation: Institute for Clinical and Experimental Virology, Free University Berlin, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Sawitzky
authors.forename: A
authors.initials: A
authors.lastname: Voigt
authors.forename: K O
authors.initials: KO
authors.lastname: Habermehl
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000990:Antithrombin III / Q000494:pharmacology; D001665:Binding Sites / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D016384:Consensus Sequence; D006493:Heparin / Q000378:metabolism*; D011558:Herpesvirus 1, Suid / Q000737:chemistry / Q000378:metabolism*; D015151:Immunoblotting; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000138:chemical synthesis / Q000378:metabolism*
chemical_list: D009842:Oligopeptides; D011956:Receptors, Cell Surface; D014759:Viral Envelope Proteins; C037680:heparin receptor; C042966:pseudorabies virus glycoproteins; D000990:Antithrombin III; D006493:Heparin