title: [Morphological change in the retina after injection of calcium and netilmicin sulfate in the rabbit vitreous body].
abstract: We reported previously selective damage of the outer segment of the photoreceptors and retinal pigment epithelium after intravitreal injection of netilmicin sulfate in the rabbit. The cellular damage was thought due to active damage of phospholipase C enzyme, preventing it from performing phospholipid inositol metabolism, and to the presence of calcium. Therefore we investigated the retinal damage following after intravitreal injection of calcium and netilmicin sulfate in the rabbit. Morphologically, the retina did not show any structural change after intravitreal injection of CaCl2 at any concentration. After an intravitreal injection of netilmicin sulfate mixed with CaCl2, the damage in the photoreceptor outer segment was reduced at a concentration of 200 micrograms/0.1 ml and in the retinal pigment epithelium at a concentration of 1,000 micrograms/0.1 ml. This showed that retinal damage due to netilmicin sulfate can be reduced by the addition of calcium.
authors.affiliation: Department of Ophthalmology, Kansai Medical University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takeichi
authors.forename: K
authors.initials: K
authors.lastname: Miki
authors.forename: M
authors.initials: M
authors.lastname: Uyama
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D007267:Injections; D009428:Netilmicin / Q000009:adverse effects / Q000494:pharmacology*; D010786:Photoreceptor Cells / Q000187:drug effects / Q000648:ultrastructure; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000648:ultrastructure; D011817:Rabbits; D012160:Retina / Q000187:drug effects* / Q000648:ultrastructure*; D014822:Vitreous Body
chemical_list: D009428:Netilmicin; D002118:Calcium