Sequence analysis of the rDNA spacer of Paracentrotus lividus and observations about pre-rRNA processing. NTS sequence of Paracentrotus lividus rDNA.
We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchin Paracentrotus lividus. This region is about 3.8 Kb long. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S rRNA precursor while has the same 5' terminus as the 32S primary precursor, also in developmental stages characterized by a low rate of rRNA synthesis.
Cantone
M
M
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
Barbieri
R
R
Duro
G
G
Izzo
V
V
Giudice
G
G
D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D015183:Restriction Mapping; D012617:Sea Urchins / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004275:DNA, Ribosomal; D012322:RNA Precursors