title: Rat liver cytosolic retinal dehydrogenase: comparison of 13-cis-, 9-cis-, and all-trans-retinal as substrates and effects of cellular retinoid-binding proteins and retinoic acid on activity.
abstract: A basic pI retinal dehydrogenase has been purified recently that accounts for approximately 90% of the all-trans-retinal dehydrogenase activity of rat liver cytosol. In this work, we show that this enzyme also accounts for approximately 90% of the 9-cis-retinal dehydrogenase activity of rat liver cytosol. The partially-purified enzyme displayed allosteric kinetics for 9-cis-retinal [K0.5 = 5.2 microM, Hill coefficient = 1.4, Vmax = 7.85 nmol min-1 (mg of protein)-1] with the ratio Vmax/K0.5 = 1.5. The latter is similar to that of 2.1 for all-trans-retinal [K0.5 = 1.6 microM, Hill coefficient = 1.4, Vmax = 3.4 nmol min-1 (mg of protein)-1]. Competition between all-trans- and 9-cis-retinal occurred only when micromolar concentrations of both were present, indicating that the dehydrogenase could catalyze both all-trans- and 9-cis-retinoic acid syntheses simultaneously at the nanomolar amounts of the retinals that are likely to occur physiologically. Although reactions of all-trans- and 9-cis-retinoids were catalyzed with similar efficiencies, 13-cis-retinal was not an efficient substrate. This retinal dehydrogenase was not feedback-inhibited by all-trans- or 9-cis-retinoic acid, nor by holocellular retinoic acid-binding protein, but was stimulated modestly by apocellular retinoic acid-binding protein, an effect not observed in the presence of cellular retinol-binding protein. These data indicate that products, via feedback inhibition, do not regulate retinoic acid synthesis by this dehydrogenase. This dehydrogenase may serve as a common enzyme in the conversion of all-trans- and 9-cis-retinal into their acids.
authors.affiliation: Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo 14214.
authors.forename: Z
authors.initials: Z
authors.lastname: el Akawi
authors.forename: J L
authors.initials: JL
authors.lastname: Napoli
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D000494:Allosteric Regulation; D000818:Animals; D001667:Binding, Competitive; D003600:Cytosol / Q000201:enzymology; D007526:Isoelectric Point; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D050697:Retinal Dehydrogenase; D012172:Retinaldehyde / Q000737:chemistry / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000494:pharmacology*; D054840:Retinol-Binding Proteins, Cellular; D013237:Stereoisomerism; D013379:Substrate Specificity; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D014212:Tretinoin; D000445:Aldehyde Oxidoreductases; D050697:Retinal Dehydrogenase; D012172:Retinaldehyde