A novel member of the interferon receptor family complements functionality of the murine interferon gamma receptor in human cells.
Expression of the human interferon gamma receptor (IFN-gamma R) in mouse cells is not sufficient to confer biological responsiveness to human IFN-gamma and vice versa. An additional species-specific component is required for signal transduction. We identified this cofactor by expression cloning in simian COS cells stably transfected with the nonfunctional murine IFN-gamma R and a IFN-gamma-inducible reporter construct encoding the human Tac antigen (interleukin-2 receptor alpha chain, CD25). A cDNA clone was obtained that, upon stable transfection, rendered human HEp-2 cells expressing the murine IFN-gamma R fully responsive to murine IFN-gamma. This cDNA encodes a novel 332 amino acid type I transmembrane protein that belongs to the IFN receptor family and that we designate IFN-gamma R beta chain.
Hemmi
S
S
Institute of Molecular Biology I, University of Zürich, Switzerland.
Böhni
R
R
Stark
G
G
Di Marco
F
F
Aguet
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D005816:Genetic Complementation Test; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017471:Receptors, Interferon / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D000096743:Interferon gamma Receptor
D018076:DNA, Complementary; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor