Cloning, sequencing, and expression in Escherichia coli of the gene encoding a 45-kilodalton protein, elongation factor Tu, from Chlamydia trachomatis serovar F.
The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibody GP-45 and cross-reacted with a monospecific polyclonal antibody to E. coli EF-Tu. Purified recombinant 45K has the capability to bind GDP, and the binding was enhanced in the presence of E. coli elongation factor Ts (EF-Ts). The GDP binding was specifically inhibited by the monoclonal antibody GP-45. These data suggest that the 45K is a chlamydial EF-Tu, and it forms a functional complex with E. coli EF-Ts protein.
Zhang
Y X
YX
Maxwell Finland Laboratory for Infectious Diseases, Boston City Hospital, Boston University School of Medicine, Massachusetts 02118.
Shi
Y
Y
Zhou
M
M
Petsko
G A
GA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D003429:Cross Reactions; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006153:Guanosine Diphosphate / Q000378:metabolism; D008969:Molecular Sequence Data; D010441:Peptide Chain Elongation, Translational; D010444:Peptide Elongation Factor Tu / Q000235:genetics* / Q000378:metabolism; D010445:Peptide Elongation Factors / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D010445:Peptide Elongation Factors; D011994:Recombinant Proteins; C023512:elongation factor Ts; D006153:Guanosine Diphosphate; D010444:Peptide Elongation Factor Tu