Effects of in vitro adrenocorticotrophic hormone, cortisol and human recombinant interleukin-2 on porcine neutrophil migration and luminol-dependent chemiluminescence.
Experiments were performed on isolated neutrophils tested in vitro to investigate the role of adrenocorticotrophic hormone (ACTH), cortisol and recombinant human interleukin-2 (rhIL-2) in modulating porcine polymorphonuclear (PMN) leukocyte function. Elevated levels of cortisol, similar to that observed during stressful experiences, caused a decrease in PMN chemokinesis (P < 0.02), chemotaxis (P < 0.001) and luminol-dependent chemiluminescence. ACTH at concentrations reflective of high stress conditions reduced PMN chemotaxis (P < 0.02) and chemokinesis (P < 0.01). In vitro rhIL-2 reversed (P < 0.05) the cortisol-induced and ACTH-induced suppression of PMN chemokinesis. These data indicate that specific neutrophil functions in pigs may be impaired by stress-related hormones of the pituitary-adrenal axis and that IL-2 may reverse some endocrine-induced suppression in chemokinesis.
Salak
J L
JL
Department of Animal Science, Texas Tech University, Lubbock 79409.
McGlone
J J
JJ
Lyte
M
M
D000324:Adrenocorticotropic Hormone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006854:Hydrocortisone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine / Q000276:immunology*
D007376:Interleukin-2; D011994:Recombinant Proteins; D008165:Luminol; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone