Isolation and characterization of a 36-kDa microfibril-associated glycoprotein by the newly synthesized isoquinolinesulfonamide affinity chromatography.
We have reported here the biochemical characterization of a newly identified microfibril-associated protein of 36-kDa (36-kDa MAP) from bovine aorta. Using Ca(2+)-dependent affinity chromatography on an isoquinolinesulfonamide derivative (CKA-1303)-coupled Sepharose, we obtained a pure form of 36-kDa MAP. This compound should serve as a useful tool for clarifying the physiological roles of 36-kDa MAP. In addition, 45Ca-autoradiography clearly indicated that 36-kDa MAP binds Ca2+. Partial amino acid sequence of 36-kDa MAP was determined. Our search of that published sequence against a 36-kDa MAP sequence revealed the resemblance to fibrinogen-related proteins of sea cucumber, cytotactin and tenascin which are substrate-adhesion proteins made at critical stages of histogenesis.
Kobayashi
R
R
Department of Pharmacology, Nagoya University School of Medicine, Japan.
Mizutani
A
A
Hidaka
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D002934:Cinnamates / Q000138:chemical synthesis; D003285:Contractile Proteins; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007546:Isoquinolines / Q000138:chemical synthesis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D000072260:RNA Splicing Factors; D017386:Sequence Homology, Amino Acid
D002135:Calcium-Binding Proteins; D002934:Cinnamates; D003285:Contractile Proteins; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D007202:Indicators and Reagents; D007546:Isoquinolines; D010446:Peptide Fragments; D000072260:RNA Splicing Factors; C016598:microfibrillar protein; C086018:CKA 1303; D002118:Calcium