title: Cellular thymidine kinase activity is required for the inhibition of HIV-1 replication by AZT in lymphocytes.
abstract: In this report, we provide evidence that the cellular thymidine kinase activity is required for the inhibition of HIV replication by zidovudine (AZT) in lymphocytes. The HSV-1 thymidine kinase protein is not able to substitute for the cellular enzyme, in accord with in vitro data that AZT is poorly phosphorylated by the HSV-derived activity.
authors.affiliation: Department of Medicine, Columbia University, New York, New York 10032.
authors.forename: I
authors.initials: I
authors.lastname: Lowy
authors.forename: M
authors.initials: M
authors.lastname: Caruso
authors.forename: S P
authors.initials: SP
authors.lastname: Goff
authors.forename: D
authors.initials: D
authors.lastname: Klatzmann
mesh_terms: D054306:Dideoxynucleotides; D004352:Drug Resistance, Microbial; D015497:HIV-1 / Q000187:drug effects* / Q000254:growth & development; D008214:Lymphocytes / Q000201:enzymology* / Q000382:microbiology; D013937:Thymidine Kinase / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects; D015215:Zidovudine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D054306:Dideoxynucleotides; D013942:Thymine Nucleotides; D015215:Zidovudine; C052873:zidovudine triphosphate; D013937:Thymidine Kinase