title: Synthesis and characterization of oligonucleotides containing the alpha-anomer of deoxyadenosine to study its influence on DNA replication.
abstract: The alpha-anomer of 2'-deoxyadenosine 1 is a major adenine lesion produced by hydroxyl radicals in DNA. To assess its biochemical effects on DNA replication, 1 was site-specifically incorporated into oligonucleotide templates using phosphoramidite chemistry. The templates were purified and extensively characterized to ensure their homogeneity and nucleotide sequences.
authors.affiliation: Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ide
authors.forename: M
authors.initials: M
authors.lastname: Okagami
authors.forename: H
authors.initials: H
authors.lastname: Murayama
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Makino
mesh_terms: D001483:Base Sequence; D004261:DNA Replication / Q000187:drug effects*; D003839:Deoxyadenosines / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D013698:Templates, Genetic
chemical_list: D003839:Deoxyadenosines; D009841:Oligonucleotides; C058118:2'-deoxyadenosine