[DNA probes for the alternative splicing region of the 6th exon of the human CSF-1 gene. Polymerase chain reaction and subcloning].
The DNA probes--pA6-CSF-1 and pA2-CSF-1 for the alternative splicing region of the 6 exon human CSF-1 gene were prepared using PCR and subsequent subcloning in pUC19 plasmid at the XmaI/BamHI sites. Due to the insert sequencing and blotting of human leukocytes DNA, the DNA probes obtained can be useful for screening of mutations in the human CSF-1 gene.
Lanina
T P
TP
Aleksandrova
N M
NM
Surin
V L
VL
Iasenskaia
E V
EV
Grineva
N I
NI
D017398:Alternative Splicing; D001483:Base Sequence; D001782:Blood Donors; D003001:Cloning, Molecular; D015342:DNA Probes; D005091:Exons; D006402:Hematologic Diseases / Q000235:genetics*; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000235:genetics*
D015342:DNA Probes; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor
DNK-zondy na oblast' al'ternativnogo splaĭsinga 6-go ekzona gena CSF-1 cheloveka. Polimeraznaia tsephaia reaktsiia i subklonirovanie.