High resolution visual mapping of stretched DNA by fluorescent hybridization.
We describe a method for stretching DNA, which, when combined with fluorescent hybridization procedures, forms a new mapping technology that produces a high resolution, vivid, multi-colour image and map. Restriction fragments and cosmid probes were successfully mapped by this procedure with validation by standard restriction mapping. A long range map of a > 200 kilobase region containing five copies of the amplified dihydrofolate reductase gene was easily generated within two days. This DNA mapping procedure offers a significant and rapid alternative to a variety of standard mapping procedures.
Parra
I
I
Cancer Therapy and Research Center, San Antonio, Texas 78229.
Windle
B
B
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D016386:Chromosome Walking; D003360:Cosmids; D006224:Cricetinae; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D015342:DNA Probes; D005796:Genes; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013314:Stress, Mechanical; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
D015342:DNA Probes; D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase