title: A chemical screening strategy for the dereplication and prioritization of HIV-inhibitory aqueous natural products extracts.
abstract: A relatively high percentage (ca. 15%) of aqueous extracts from terrestrial plants, cyanobacteria, and marine invertebrates and algae has exhibited activity in the National Cancer Institute's primary AIDS-antiviral screen. By removal of anionic polysaccharides in a first stage of dereplication, we have eliminated from further consideration a considerable number of these extracts. However, a still substantial proportion of the active extracts remained, from which we wished to select and prioritize a small percentage for our detailed bioassay-directed fractionation studies. Therefore, a chemical screening protocol, utilizing various solid-phase extraction cartridges, has been developed for a second-stage dereplication and to assist in prioritization of these extracts for our further investigations.
authors.affiliation: Laboratory of Drug Discovery Research and Development, National Cancer Institute, Frederick, MD 21702-1201.
authors.forename: J H
authors.initials: JH
authors.lastname: Cardellina
authors.forename: M H
authors.initials: MH
authors.lastname: Munro
authors.forename: R W
authors.initials: RW
authors.lastname: Fuller
authors.forename: K P
authors.initials: KP
authors.lastname: Manfredi
authors.forename: T C
authors.initials: TC
authors.lastname: McKee
authors.forename: M
authors.initials: M
authors.lastname: Tischler
authors.forename: H R
authors.initials: HR
authors.lastname: Bokesch
authors.forename: K R
authors.initials: KR
authors.lastname: Gustafson
authors.forename: J A
authors.initials: JA
authors.lastname: Beutler
authors.forename: M R
authors.initials: MR
authors.lastname: Boyd
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D013634:Tannins / Q000302:isolation & purification / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D013634:Tannins