[The effect of adrenaline on the activity and functional mobility of transhydrogenase in the inner mitochondrial membrane].
Adrenaline injection to rats increased activity of liver mitochondrial transhydrogenase. Transhydrogenase in the adrenaline activated submitochondrial particles showed increased sensitivity to thermal and proteolytic inactivations as well as to inhibition by dicyclohexyl carbodiimide. Substrates of the reaction NAD+ and NADPH, which induce different conformational changes of the enzyme molecule in membranes, alternated to a greater extent the sensitivity to these treatments of transhydrogenase in the adrenaline activated submitochondrial particles as compared with controls. Activation of transhydrogenase by catecholamines appears to occur due to increase of the enzyme molecule mobility in the inner mitochondrial membrane.
Medvedev
A E
AE
D000818:Animals; D004789:Enzyme Activation; D004837:Epinephrine / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009250:NADP Transhydrogenases / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013367:Submitochondrial Particles / Q000187:drug effects / Q000201:enzymology
D009243:NAD; D009249:NADP; D009250:NADP Transhydrogenases; D004837:Epinephrine
Vliianie adrenalina na aktivnost' i funktsional'nuiu podvizhnost' transgidrogenazy vo vnutrenneĭ membrane mitokhondriĭ.