The high-affinity Ca(2+)-ATPase from rat parotid plasma membranes is an ectoenzyme: solubilization and characterization of the Ca(2+)-ATPase activity.
A high-affinity Ca(2+)-ATPase was solubilized from rat parotid plasma membranes and purified by concanavalin A and DEAE-cellulose chromatography. The properties of the purified high-affinity Ca(2+)-ATPase are very different from those of the parotid plasma membrane ATP-dependent Ca2+ pump but appear to be similar to those of a rat liver cell adhesion protein which exhibits high-affinity ecto-Ca(2+)-ATPase activity.
Teo
T S
TS
Department of Biochemistry, National University of Singapore, Kent Ridge.
Thiyagarajah
P
P
Lee
M K
MK
Selwyn
M J
MJ
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000302:isolation & purification* / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D010306:Parotid Gland / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar
D000252:Calcium-Transporting ATPases