Evolution of RNA editing in kinetoplastid protozoa.
The editing of RNA in trypanosomatid mitochondria involves the insertion and occasional deletion of uridine residues within coding regions of maxicircle messenger RNA transcripts. The extent to which the transcripts of homologous genes undergo editing differs in different species. In some, entire genes are edited (pan-editing), whereas in others, editing is limited to the 5' termini of editing domains (5' editing). Here we investigate which type of editing is ancestral and which is derived, by analysing RNA editing in the different lineages, using a kinetoplastid phylogeny reconstructed from nuclear small subunit ribosomal RNA sequences. We conclude that the ancestral cryptogenes were pan-edited, and we hypothesize that the 5'-edited homologues were generated by several independent events from partially edited RNAs, in which case editing may be a more primitive mechanism than previously thought.
Maslov
D A
DA
Department of Biology, University of California, Los Angeles 90024.
Avila
H A
HA
Lake
J A
JA
Simpson
L
L
D000251:Adenosine Triphosphatases / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003576:Electron Transport Complex IV / Q000235:genetics; D017125:Genes, Protozoan; D016830:Kinetoplastida / Q000145:classification / Q000235:genetics*; D025261:Mitochondrial Proton-Translocating ATPases; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D010086:Oxidative Phosphorylation Coupling Factors; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics*
C086201:MURF4 protein, Protozoa; D010086:Oxidative Phosphorylation Coupling Factors; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases; C000628217:F(6) ATPase; D025261:Mitochondrial Proton-Translocating ATPases