Cloning, sequencing and expression of cDNA encoding an insect V-ATPase subunit E.
This work presents the first invertebrate cDNA sequence encoding subunit E of a V-ATPase. It was cloned by immuno-shot-gun screening of a Manduca sexta (Insecta, Lepidoptera, Sphingidae) posterior larval midgut cDNA library. The amino acid sequence was 64% identical to that of the mammalian E-subunit and 34% to that of yeast. Southern and Northern blots suggested the existence of only one gene encoding the insect subunit E.
Gräf
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R
Zoologisches Institut der Universität, München, Germany.
Harvey
W R
WR
Wieczorek
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H
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D007313:Insecta / Q000201:enzymology*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D025262:Vacuolar Proton-Translocating ATPases
D018076:DNA, Complementary; D000251:Adenosine Triphosphatases; D025262:Vacuolar Proton-Translocating ATPases