The effect of cefepime on some immune parameters in vitro: lack of interference with mitogen-induced lymphoproliferation, immunoglobulin synthesis, IFN-gamma and IL-2 secretion and IL-2 receptor expression.
The possible interference of the novel antibiotic cefepime (CPE) with some functions of the immune system was investigated in vitro. Peripheral blood mononuclear cells (PBMC) cultured in the presence of drug concentrations ranging from 25 to 100 micrograms/ml normally maintained their responsiveness to polyclonal (PHA, Con A, PWM) mitogenic stimulation in regard to proliferative response, IgM and IgG synthesis and IFN-gamma and IL-2 secretory capacity. Moreover, PHA-induced expression of IL-2 receptors was comparable in PBMCs cultured in the presence or absence of CPE. Taken together, these data suggest that CPE does not interfere, at this specific level, with T- and B-cell mediated functions in vitro.
Di Marco
R
R
Institute of Microbiology and Virology, University of Catania, Italy.
Carrabba
I
I
Cavallaro
V
V
Zaccone
P
P
Stazzone
C
C
Franco
S
S
Cocuzza
C
C
Nicoletti
G
G
Nicoletti
F
F
D000328:Adult; D000077723:Cefepime; D002511:Cephalosporins / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D015375:Receptors, Interleukin-2 / Q000032:analysis*
D002511:Cephalosporins; D007136:Immunoglobulins; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D000077723:Cefepime; D007371:Interferon-gamma