title: [Analysis of proliferating cell nuclear antigen and nucleolar organizer region in testicular tumors].
abstract: Immunohistochemical staining using the monoclonal antibody of proliferating cell nuclear antigen (PCNA) and argyrophilic nucleolar organizer region associated protein (AgNOR) staining were performed on 28 paraffin-embedded testicular tumors, and their correlation was assessed according to histological type, clinical staging and tumor markers. PCNA positive rates and AgNOR numbers in nuclei were 57.9%, 4.38 in seminomas, 63.4%, 5.79 in embryonal carcinomas, 37.7%, 2.63 in teratomas, 61.3%, 4.43 in yolk sac tumors, 79.5%, 7.12 in choriocarcinomas, and 20.9%, 3.03 in cells of the normal seminiferous tubule, respectively. In pure seminomas, the PCNA positive rates and AgNOR numbers of the stage II to III group (72.6%, 4.75) were higher than those of the stage I group (47.8%, 3.67), but there was no significant difference between the two groups. There was a significant correlation between PCNA positive rate and AgNOR number in all specimens (r = 0.76, p < 0.002). These findings indicate that PCNA and AgNOR are suitable indicators for proliferative activity of testicular tumors.
authors.affiliation: Department of Urology, Yamaguchi University School of Medicine.
authors.forename: H
authors.initials: H
authors.lastname: Kurisu
authors.forename: T
authors.initials: T
authors.lastname: Shimabukuro
authors.forename: T
authors.initials: T
authors.lastname: Wada
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: I A
authors.initials: IA
authors.lastname: Shameem
authors.forename: T
authors.initials: T
authors.lastname: Otsuka
authors.forename: S
authors.initials: S
authors.lastname: Sakano
authors.forename: H
authors.initials: H
authors.lastname: Matsuyama
authors.forename: K
authors.initials: K
authors.lastname: Naito
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000032:analysis*; D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor / Q000032:analysis; D002455:Cell Division; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D018809:Proliferating Cell Nuclear Antigen; D016622:Silver Staining; D013736:Testicular Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm; D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; C051963:nucleolar organizer region associated proteins