Rapid detection of PCR products of hepatitis C virus (HCV) with a non-radioactive oligoprobe.
Dot-blot for rapid detection of PCR amplification products of hepatitis C virus (HCV) using a digoxigenin (DIG)-labelled oligoprobe was developed and its sensitivity compared with Southern blot hybridization. The specificity and sensitivity of the DIG-labelled probe were identical to those of the 32P-labelled when the DIG-labelled nucleic acids were detected by enzyme-catalyzed chemiluminescent reaction. The lack of radioactivity makes this procedure suitable for routine use in diagnostic laboratories.
Canepari
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P
Istituto di Microbiologia dell'Università di Verona, Italy.
Di Stefano
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CF
Lleò
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MM
D001483:Base Sequence; D015139:Blotting, Southern; D002908:Chronic Disease; D004076:Digoxigenin; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes; D012367:RNA, Viral; D004076:Digoxigenin