Murine dendritic cells pulsed in vitro with tumor antigen induce tumor resistance in vivo.
The aim of this work is to induce tumor resistance to a B cell lymphoma in BALB/c mice using elements of the immune system. It has indeed been shown by us and by others that antigen-presenting cells (APC) like dendritic cells can induce efficient immune responses and can even substitute for Freund's adjuvant. Here we show that mice immunized with syngeneic dendritic cells pulsed in vitro with tumor antigen (BCL1 idiotype expressed by lymphoma cells) are protected against a subsequent tumor inoculation. The in vivo resistance can be correlated with the induction of a humoral response specific for the idiotype expressed by the tumor. No such protection can be achieved when B cells are used as APC. These data show that effector cells in tumor-bearing animals can be recruited and activated using dendritic cells, providing long-lasting immune surveillance.
Flamand
V
V
Département de Biologie Moléculaire, Université Libre de Bruxelles, Belgium.
Sornasse
T
T
Thielemans
K
K
Demanet
C
C
Bakkus
M
M
Bazin
H
H
Tielemans
F
F
Leo
O
O
Urbain
J
J
Moser
M
M
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D003713:Dendritic Cells / Q000276:immunology*; D005803:Genes, Immunoglobulin; D007111:Immunity, Cellular; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D007167:Immunotherapy; D016393:Lymphoma, B-Cell / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D013154:Spleen / Q000473:pathology; D016019:Survival Analysis
D017931:DNA Primers; D007130:Immunoglobulin Idiotypes; D007148:Immunoglobulin mu-Chains