title: Local immunotherapy of recurrent glioblastoma multiforme by intracerebral perfusion of interleukin-2 and LAK cells.
abstract: A non randomized pilot study has been undertaken to evaluate the feasibility of local immunotherapy (IT) of recurrent glioblastoma multiforme (GM) by continuous intracerebral perfusion of recombinant interleukin-2 (rIL-2, Eurocetus) with and without lymphokine activated killer (LAK) cells. At time of surgical removal of the tumor, a catheter was implanted in the cavity left by tumor debulking allowing continuous perfusion of rIL-2. Five patients received 18 x 10(6) IU/day or rIL-2 for five days. At days 1, 3, and 5 after surgery, rIL-2 perfusion was briefly interrupted for the injection of LAK cells. Eight other patients received rIL-2 alone, either 24 x 10(6) IU/day (five patients) or 54 x 10(6) IU/day (three patients). Capillary leak syndrome, which is the main side effect of systemic infusion of rIL-2, was never observed, but local immunotherapy induced fever, confusion, and cerebral edema in all patients. Despite local IT, tumor progression was diagnosed by CT scan 4 to 12 weeks after the treatment.
authors.affiliation: Centre Régional de Transfusion Sanguine, CHU Purpan, Toulouse, France.
authors.forename: A
authors.initials: A
authors.lastname: Blancher
authors.forename: F
authors.initials: F
authors.lastname: Roubinet
authors.forename: A S
authors.initials: AS
authors.lastname: Grancher
authors.forename: M
authors.initials: M
authors.lastname: Tremoulet
authors.forename: A
authors.initials: A
authors.lastname: Bonaté
authors.forename: M B
authors.initials: MB
authors.lastname: Delisle
authors.forename: J P
authors.initials: JP
authors.lastname: Calot
authors.forename: C
authors.initials: C
authors.lastname: Pourreau
authors.forename: C
authors.initials: C
authors.lastname: Franks
authors.forename: J
authors.initials: J
authors.lastname: Ducos
mesh_terms: D000328:Adult; D000368:Aged; D001921:Brain; D001932:Brain Neoplasms / Q000628:therapy*; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005500:Follow-Up Studies; D005909:Glioblastoma / Q000628:therapy*; D006801:Humans; D007376:Interleukin-2 / Q000008:administration & dosage*; D015979:Killer Cells, Lymphokine-Activated / Q000637:transplantation*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000628:therapy*; D010477:Perfusion; D010865:Pilot Projects
chemical_list: D007376:Interleukin-2