Cloning and sequencing of the Leishmania major actin-encoding gene.
We report the cloning and characterization of the genomic sequence of the actin (Act)-encoding gene (act) from Leishmania major. Restriction maps of two genomic clones, as well as genomic Southern analysis strongly suggest that the act of L. major is a single-copy gene. A single 1.6-kb transcript is detected in Northern blots. The deduced amino-acid sequence shows 68-89% identity with Act sequences from other eukaryotes.
de Arruda
M V
MV
Whitehead Institute for Biomedical Research, Cambridge, MA.
Matsudaira
P
P
D000199:Actins / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D015870:Gene Expression; D017125:Genes, Protozoan; D006801:Humans; D018320:Leishmania major / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D000199:Actins