Isolation and characterization of cDNAs encoding wheat 3-hydroxy-3-methylglutaryl coenzyme A reductase.
The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC 1.1.1.34) is a key enzyme in the isoprenoid biosynthetic pathway. We have isolated partial cDNAs from wheat (Triticum aestivum) using the polymerase chain reaction. Comparison of deduced amino acid sequences of these cDNAs shows that they represent a small family of genes that share a high degree of sequence homology among themselves as well as among genes from other organisms including tomato, Arabidopsis, hamster, human, Drosophila, and yeast. Southern blot analysis reveals the presence of at least four genes. Our results concerning the tissue-specific expression as well as developmental regulation of these HMGR cDNAs highlight the important role of this enzyme in the growth and development of wheat.
Aoyagi
K
K
Sogetal, Incorporated, Hayward, California 94545.
Beyou
A
A
Moon
K
K
Fang
L
L
Ulrich
T
T
D000595:Amino Acid Sequence; D000818:Animals; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D017343:Genes, Plant; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014908:Triticum / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development
D018076:DNA, Complementary; D012333:RNA, Messenger; D006903:Hydroxymethylglutaryl CoA Reductases