Detection of Candida albicans and other yeasts in blood by PCR.
Primers complementary to the region of genes coding for rRNA in Candida albicans were used in PCRs to detect yeast DNA extracted from blood samples containing various Candida species. One fragment (105 bp) was amplified from all yeasts tested, whereas a second (684 bp) was only amplified when C. albicans DNA was present. The level of sensitivity was 15 +/- 5 (mean +/- standard error) CFU of C. albicans per ml of blood.
Holmes
A R
AR
Department of Oral Biology and Oral Pathology, School of Dentistry, University of Otago, Dunedin, New Zealand.
Cannon
R D
RD
Shepherd
M G
MG
Jenkinson
H F
HF
D001483:Base Sequence; D002175:Candida / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000097:blood / Q000175:diagnosis / Q000235:genetics; D004271:DNA, Fungal / Q000097:blood* / Q000235:genetics; D004275:DNA, Ribosomal / Q000097:blood* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004271:DNA, Fungal; D004275:DNA, Ribosomal