title: Immunocytochemistry in the assessment of pS2 protein expression in fine needle aspiration cytology from breast carcinoma.
abstract: pS2 protein is a cysteine-rich polypeptide, of unknown function, the expression of which is induced in the human cancer cell line MCF-7 by oestrogen. The availability of a murine monoclonal antibody to human pS2 protein has prompted us to evaluate its expression in 47 cases of primary breast carcinoma. Using a double indirect immunoperoxidase technique, we compared the expression of pS2 protein in fine needle aspiration (FNA) cytology smears with that in formalin-fixed, paraffin-embedded sections from subsequently excised tumours from the same patients. We also compared the expression of pS2 protein and oestrogen receptor (ER) status using immunocytochemical assay (ER-ICA) in formalin-fixed, paraffin-embedded sections from 22 primary breast carcinomas. We found the application of immunocytochemistry in the assessment of pS2 protein expression in FNA cytology to be a reliable and cost-effective technique, having a sensitivity of 84% and a specificity of 100%. There was also a good correlation between the expression of pS2 protein and ER status.
authors.affiliation: Department of Histopathology, Frenchay Hospital, Bristol, UK.
authors.forename: N B
authors.initials: NB
authors.lastname: Ibrahim
authors.forename: C J
authors.initials: CJ
authors.lastname: Padfield
authors.forename: E J
authors.initials: EJ
authors.lastname: Rees
authors.forename: Y
authors.initials: Y
authors.lastname: Wilson
authors.forename: S J
authors.initials: SJ
authors.lastname: Cawthorn
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D004967:Estrogens; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011446:Prospective Studies; D011506:Proteins; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins
chemical_list: D004967:Estrogens; D009363:Neoplasm Proteins; D011506:Proteins; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins