PCR analysis of the cryI insecticidal crystal family genes from Bacillus thuringiensis.
A method allowing rapid and accurate identification of different subgroups within the insecticidal crystal CryI protein-producing family of Bacillus thuringiensis strains was established by using PCR technology. Thirteen highly homologous primers specific to regions within genes encoding seven different subgroups of B. thuringiensis CryI proteins were described. Differentiation among these strains was determined on the basis of the electrophoretic patterns of PCR products. B. thuringiensis strains, isolated from soil samples, were analyzed by PCR technology. Small amounts of bacterial lysates were assayed in two reaction mixtures containing six to eight primers. This method can be applied to rapidly detect the subgroups of CryI proteins that correspond with toxicity to various lepidopteran insects.
Ceron
J
J
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
Covarrubias
L
L
Quintero
R
R
Ortiz
A
A
Ortiz
M
M
Aranda
E
E
Lina
L
L
Bravo
A
A
D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics*; D001427:Bacterial Toxins; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins / Q000145:classification / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D008800:Mexico; D008969:Molecular Sequence Data; D005810:Multigene Family; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction / Q000379:methods*; D012988:Soil Microbiology
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis