Pancreatic phospholipase A2 in cerulein-induced acute pancreatitis in the rat.
We investigated the concentration of immunoreactive pancreatic phospholipase A2 (pan-PLA2) and the catalytic activity of phospholipase A2 (CA-PLA2) in plasma and pancreases of rats with cerulein-induced acute pancreatitis. Edematous pancreatitis with ascites and fat necroses in the abdominal cavity developed after 8 h infusion of cerulein (5 micrograms/kg/h). Large vacuoles were found in acinar cells and there were small areas of acinar cell necrosis. No pathological changes were seen in saline-infused control animals. Pancreatic PLA2 was localized by immunohistochemistry in pancreatic acinar cells in both groups of animals and in the proximal tubular cells of the kidney in cerulein-infused animals. The lungs and kidneys appeared normal by light microscopy in all animals. The pan-PLA2 values increased markedly, whereas the CA-PLA2 values did not change during the cerulein-infusion. The CA-PLA2 values in the homogenates of pancreatic tissue of cerulein-infused animals did not differ significantly from those of saline-infused controls. The results indicate that the CA-PLA2 in plasma is independent from the concentration of pan-PLA2 in cerulein-induced acute pancreatitis in rat.
Hietaranta
A J
AJ
Department of Pathology, University of Turku, Finland.
Aho
H J
HJ
Nevalainen
T J
TJ
D000208:Acute Disease; D000818:Animals; D002384:Catalysis; D002108:Ceruletide; D006400:Hematocrit; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D009994:Osmolar Concentration; D010179:Pancreas / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000201:enzymology* / Q000378:metabolism; D010741:Phospholipases A / Q000097:blood / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar
D002108:Ceruletide; D010741:Phospholipases A; D054467:Phospholipases A2