Automated column-switching high-performance liquid chromatography for the determination of 2-thiothiazolidine-4-carboxylic acid in urine.
2-Thiothiazolidine-4-carboxylic acid (TTCA) is the accepted urinary indicator of occupational exposure to carbon disulfide. An extractionless automated column-switching procedure for the determination of this compound in urine was developed. The biological fluid is directly injected into the chromatographic system after dilution. A clean-up procedure is performed in an anion-exchange pre-column, with aqueous formic acid as eluent, followed by a transfer to a cyan-amino or amino analytical column in order to carry out analysis of TTCA under isocratic elution conditions, with a mixture of acetonitrile, water and formic acid as eluent, combined with ultraviolet detection. The detection limit of the method in urine is estimated to be 0.1 mg/l.
Simon
P
P
Institut National de Recherche et de Sécurité, Vandoeuvre, France.
Nicot
T
T
D000097:Acetonitriles; D000838:Anions; D001322:Autoanalysis; D002246:Carbon Disulfide / Q000506:poisoning; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D005561:Formates; D006801:Humans; D016273:Occupational Exposure; D013844:Thiazoles / Q000652:urine*; D053778:Thiazolidines
D000097:Acetonitriles; D000838:Anions; D005561:Formates; D013844:Thiazoles; D053778:Thiazolidines; C030544:formic acid; C031677:2-thioxo-4-thiazolidinecarboxylic acid; D002246:Carbon Disulfide; C032159:acetonitrile