[Preservation of PCNA in different fixatives and demonstration of PCNA as well as membrane antigen by double labelling technique].
Four fixatives were used with two different fixation times in order to determine the influence of different fixatives on PCNA preservation. The result indicated that the aldehyde-base fixatives preserved PCNA excellently and the appropriate fixative time was 24 hours. The result of alcohol-base fixative on PCNA preservation seemed unsatisfactory and 10% buffer formalin was the best one among the aldehyde-base fixatives for this preservation. Additionally, double-labelling technique for demonstrating PCNA as well as the membrane antigen in the same slide was established. Selection of antigen chromogens suitable to double-labelling is described.
Yang
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Department of Pathology, West China University of Medical Sciences, Chengdu.
D000230:Adenocarcinoma / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology; D005404:Fixatives; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000276:immunology; D008223:Lymphoma / Q000276:immunology; D009447:Neuroblastoma / Q000276:immunology; D009687:Nuclear Proteins / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen
D000951:Antigens, Neoplasm; D001324:Autoantigens; D005404:Fixatives; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen