title: Single radial immunodiffusion potency test for standardization of indigenous equine influenza vaccine.
abstract: Single radial immunodiffusion (SRD) assays were used for measuring the haemagglutinin antigen contents of equine influenza vaccine prepared from an Indian virus isolate. A/Equine-2/Ludhiana/1/87 (H3N8). Five different preparations of the vaccine were standardized by SRD to prepare 913 doses, each containing 20 micrograms HA/ml-1 dose-1. This test also showed influenza virus subtype specificity as no cross reaction was observed between subtype 1 (H7N7) and subtype 2 (H3N8) viruses.
authors.affiliation: National Research Centre on Equines, Hisar, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Gupta
authors.forename: M P
authors.initials: MP
authors.lastname: Yadav
authors.forename: P K
authors.initials: PK
authors.lastname: Uppal
mesh_terms: D000818:Animals; D006389:Hemagglutinins, Viral / Q000032:analysis; D006734:Horse Diseases / Q000517:prevention & control; D006736:Horses; D005779:Immunodiffusion / Q000379:methods* / Q000592:standards; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000032:analysis* / Q000592:standards; D009976:Orthomyxoviridae Infections / Q000517:prevention & control / Q000662:veterinary; D012015:Reference Standards; D015164:Vaccines, Inactivated / Q000032:analysis / Q000592:standards
chemical_list: D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated