Evaluation of proliferating cells in acute leukemia with antibodies to proliferating cell nuclear antigen and Ki-67 monoclonal antibodies.
The percentage of proliferating leukemic cells was evaluated in 32 patients with acute leukemia; this percentage was measured by flow cytometry and immunostaining with antibodies to proliferating cell nuclear antigen and Ki-67 monoclonal antibodies. In the peripheral blood samples, the mean proportion of cells that stained for proliferating cell nuclear antigen was 10%, the mean proportion of cells that stained for Ki-67 was 12%, and the mean proportion of cells in the S phase was 5.9%; in the bone marrow samples, these means were 15%, 15%, and 10.1%, respectively, all of which were higher than in the peripheral blood samples. There was a correlation between the proportion of cells that stained for proliferating cell nuclear antigen and cells that stained for Ki-67, and there was no correlation between the proportion of cells that stained for proliferating cell nuclear antigen or Ki-67 and the proportion of cells in the S phase.
Inoue
T
T
Department of Laboratory Medicine, Osaka City University Medical School, Japan.
Yamane
T
T
Park
K
K
Im
T
T
Tatsumi
N
N
Okuda
K
K
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D001752:Blast Crisis / Q000473:pathology; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D007938:Leukemia / Q000473:pathology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen