[Wound healing of the corneal endothelium in vivo after intraocular injection of recombinant human bFGF].
In the present study, we investigated the potential role of recombinant human basic fibroblast growth factor (rh-bFGF) on the regeneration of corneal endothelium in the rabbit. For in vivo wound-healing experiments, standardized wounds were created by mechanically scraping the endothelial cells within the boundaries of a central epithelial trephine mark. A single dose of 1.5 micrograms rh-bFGF was injected into the anterior chamber of the right eye immediately after wounding, while the left eyes received the ointment vehicle only (n = 27 rabbits). Functional recovery and wound closure rates were assessed by means of ultrasonic pachymetry, cell counts after endothelial vital staining and direct computer-assisted surface analysis of Janus green-stained endothelial lesions. Measurements were carried out 0, 1, 2, 4, and 7 days after injury. For the time points day 2 and day 4, significant stimulation of endothelial regeneration in rh-bFGF treated eyes was observed with all parameters measured. These results demonstrate that rh-bFGF could prove to be useful in clinical conditions, e.g. to conserve endothelial function after intraocular surgery or as a supplement to corneal intermediate preservation media.
Rieck
P
P
Unité de Recherches Gérontologiques, Paris.
Hartmann
C
C
Pouliquen
Y
Y
Courtois
Y
Y
D000818:Animals; D000867:Anterior Chamber; D002452:Cell Count / Q000187:drug effects; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000293:injuries / Q000473:pathology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007267:Injections; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects / Q000502:physiology; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2
Wundheilung des Hornhautendothels in vivo nach intraokularer Injektion von rekombinantem humanem bFGF.