Phospholipase-like myotoxins induce rapid membrane leakage of non-hydrolyzable ether-lipid liposomes.
Two phospholipase-like myotoxins--ammodytin L from Vipera ammodytes and myotoxin II from Bothrops asper--are shown to be able to induce leakage of liposomes made from non-hydrolyzable ether-linked phospholipids. This demonstrates that the cytolytic activity of these toxins is completely independent of any remaining enzyme activity or contamination with active phospholipases.
Pedersen
J Z
JZ
Department of Biology, University of Rome Tor Vergata, Italy.
de Arcuri
B F
BF
Morero
R D
RD
Rufini
S
S
D003435:Crotalid Venoms / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D005454:Fluorescence Polarization; D054501:Group II Phospholipases A2; D008081:Liposomes / Q000737:chemistry*; D009498:Neurotoxins / Q000494:pharmacology*; D010741:Phospholipases A / Q000494:pharmacology*; D010742:Phospholipid Ethers / Q000737:chemistry; D030162:Reptilian Proteins; D014757:Viper Venoms / Q000494:pharmacology*
D003435:Crotalid Venoms; D008081:Liposomes; D009498:Neurotoxins; D010742:Phospholipid Ethers; D030162:Reptilian Proteins; D014757:Viper Venoms; C065419:ammodytin L; D010741:Phospholipases A; D054501:Group II Phospholipases A2; C061056:myotoxin II, Bothrops asper