Structure, function, and chromosome mapping of the growth-suppressing human homologue of the murine gas1 gene.
We describe the isolation, growth-suppressing activity, and chromosomal localization of the human homologue of the murine growth-arrest-specific gene gas1. Overexpression of h-gas1 is able to block cell proliferation in the A549 lung carcinoma and the T24 bladder carcinoma cell lines. No effect was observed when h-gas1 was introduced into the osteosarcoma cell line SAOS-2 and into the adenovirus-type-5 transformed cell line 293. This finding is related to our previous evidence that simian virus 40-transformed NIH 3T3 cells are also refractory to murine gas1 overexpression, suggesting that the retinoblastoma and/or p53 gene products have an active role in mediating the growth-suppressing effect of gas1. We also show that h-gas1 is on chromosome 9q21.3-22.1, in a region considered to be a fragile site. Altogether, the results raise the possibility that h-gas1 may be a target for genetic alterations leading to its inactivation in tumor cells.
Del Sal
G
G
Laboratoire Nazionale, Consorzio Interuniversitario per le Biotechnologie, Trieste, Italy.
Collavin
L
L
Ruaro
M E
ME
Edomi
P
P
Saccone
S
S
Valle
G D
GD
Schneider
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002455:Cell Division / Q000235:genetics; D002460:Cell Line; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D018076:DNA, Complementary / Q000235:genetics; D058851:GPI-Linked Proteins; D015870:Gene Expression; D006131:Growth Inhibitors / Q000235:genetics* / Q000502:physiology; D006801:Humans; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C066709:GAS1 protein, S cerevisiae; C096192:GAS1 protein, human; D058851:GPI-Linked Proteins; C501180:Gas1 protein, mouse; D006131:Growth Inhibitors; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins