Assignment of human thyrotropin-releasing hormone (TRH) receptor gene to chromosome 8.
The human gene encoding thyrotropin-releasing hormone receptor was assigned to chromosome 8, using human-Chinese hamster ovary somatic cell hybrids, analyzed by Southern hybridizations. Hybridization was carried out with a 32P-labeled fragment of the human TRH-R genomic DNA. Hybridization of this probe to a human specific 10.5-kb DNA fragment of EcoRI-digested WBC DNA was used to localize the human TRH-R gene. No hybridization, by contrast, was seen with this probe and hamster DNA after EcoRI treatment. Results from 18 somatic cell hybrids corroborated unequivocally that the human TRH-R gene can be assigned to human chromosome 8.
Yamada
M
M
First Department of Internal Medicine, Gunma University School of Medicine, Japan.
Monden
T
T
Konaka
S
S
Mori
M
M
D000818:Animals; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D006224:Cricetinae; D003412:Cricetulus; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000235:genetics*
D015342:DNA Probes; D018025:Receptors, Thyrotropin-Releasing Hormone