title: Influence of cytoplasmic microinjection on meiotic competence in growing pig oocytes.
abstract: It is demonstrated that the microinjection of cytoplasm from competent, fully grown oocytes can induce germinal vesicle breakdown in meiotically incompetent growing oocytes. Injection of cytoplasm from oocytes at methaphase I had a similar effect. The maturation in injected growing oocytes was arrested at the premetaphase or metaphase I stage. Induction of germinal vesicle breakdown by cytoplasm microinjection occurs in a dose-dependent manner.
authors.affiliation: Research Institute of Animal Production, Prague, Uhrínĕves, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Petr
authors.forename: J
authors.initials: J
authors.lastname: Rozinek
authors.forename: J
authors.initials: J
authors.lastname: Fulka
authors.forename: F
authors.initials: F
authors.lastname: Jílek
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000502:physiology*; D005260:Female; D008540:Meiosis / Q000502:physiology*; D008677:Metaphase; D008845:Microinjections; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure*; D013552:Swine