[Analysis of proliferating cell nuclear antigen and nucleolar organizer region in testicular tumors].
Immunohistochemical staining using the monoclonal antibody of proliferating cell nuclear antigen (PCNA) and argyrophilic nucleolar organizer region associated protein (AgNOR) staining were performed on 28 paraffin-embedded testicular tumors, and their correlation was assessed according to histological type, clinical staging and tumor markers. PCNA positive rates and AgNOR numbers in nuclei were 57.9%, 4.38 in seminomas, 63.4%, 5.79 in embryonal carcinomas, 37.7%, 2.63 in teratomas, 61.3%, 4.43 in yolk sac tumors, 79.5%, 7.12 in choriocarcinomas, and 20.9%, 3.03 in cells of the normal seminiferous tubule, respectively. In pure seminomas, the PCNA positive rates and AgNOR numbers of the stage II to III group (72.6%, 4.75) were higher than those of the stage I group (47.8%, 3.67), but there was no significant difference between the two groups. There was a significant correlation between PCNA positive rate and AgNOR number in all specimens (r = 0.76, p < 0.002). These findings indicate that PCNA and AgNOR are suitable indicators for proliferative activity of testicular tumors.
Kurisu
H
H
Department of Urology, Yamaguchi University School of Medicine.
Shimabukuro
T
T
Wada
T
T
Yamamoto
M
M
Shameem
I A
IA
Otsuka
T
T
Sakano
S
S
Matsuyama
H
H
Naito
K
K
Yamamoto
N
N
D000328:Adult; D000951:Antigens, Neoplasm / Q000032:analysis*; D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor / Q000032:analysis; D002455:Cell Division; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D018809:Proliferating Cell Nuclear Antigen; D016622:Silver Staining; D013736:Testicular Neoplasms / Q000378:metabolism* / Q000473:pathology
D000951:Antigens, Neoplasm; D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; C051963:nucleolar organizer region associated proteins