title: Virus-associated down-regulation of the gene encoding cryparin, an abundant cell-surface protein from the chestnut blight fungus, Cryphonectria parasitica.
abstract: We report here the cloning and analysis of the gene (Crp) encoding cryparin (CRP) and the suppression of the accumulation of its mRNA by a double-stranded RNA virus. CRP belongs to a group of fungal hydrophobic cell surface proteins and has sequence similarity to cerato-ulmin, a phytotoxin produced by Ophiostoma ulmi, the Dutch elm disease pathogen. Transcripts of the Crp gene represent almost 25% of the mRNA of the fungal cell during the peak of CRP production.
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station 77843.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Villalon
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.forename: P
authors.initials: P
authors.lastname: Kazmierczak
authors.forename: N K
authors.initials: NK
authors.lastname: van Alfen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002462:Cell Membrane / Q000378:metabolism; D016384:Consensus Sequence; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007438:Introns; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014780:Viruses / Q000235:genetics*; D013098:Xylariales / Q000235:genetics* / Q000378:metabolism
chemical_list: C074967:CRP protein, Cryphonectria parasitica; D004271:DNA, Fungal; D005656:Fungal Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger