title: Confirmation of the localization of the human recombination activating gene 1 (RAG1) to chromosome 11p13.
abstract: The human recombination activating gene 1 (RAG1) has previously been mapped to chromosomes 14q and 11p. Here we confirm the chromosome 11 assignment by two independent approaches: autoradiographic and fluorescence in situ hybridization to metaphase spreads and analysis of human-hamster somatic cell hybrid DNA by the polymerase chain reaction (PCR) and Southern blotting. Our results unequivocally localize RAG1 to 11p13.
authors.affiliation: Sektion Molekularbiologie, Universität Ulm, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Schwarz
authors.forename: H
authors.initials: H
authors.lastname: Hameister
authors.forename: M
authors.initials: M
authors.lastname: Gessler
authors.forename: K H
authors.initials: KH
authors.lastname: Grzeschik
authors.forename: T E
authors.initials: TE
authors.lastname: Hansen-Hagge
authors.forename: C R
authors.initials: CR
authors.lastname: Bartram
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods; D001483:Base Sequence; D015139:Blotting, Southern; D016466:CHO Cells; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D006224:Cricetinae; D017931:DNA Primers / Q000737:chemistry; D016654:Genes, RAG-1; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers