Isolation and characterization of a gene from Trypanosoma cruzi encoding a 46-kilodalton protein with homology to human and rat tyrosine aminotransferase.
The complete sequence of a gene encoding a 46-kDa protein of Trypanosoma cruzi is presented. The first ATG complies with the consensus sequence for initiation of translation. A single band of 2 kb was highlighted by hybridizing a probe from the 46-kDa protein gene to a Northern filter containing total T. cruzi RNA. The gene is present in 50-80 copies per cell and most of them are contained in 2 tandem arrays on large T. cruzi chromosomes (> 2000 kb). A strong homology with rat and human tyrosine aminotransferase was detected. Homology with a Trypanosoma brucei retrotransposon was found in the nonsense strand of the intergenic region.
Bontempi
E J
EJ
Instituto Nacional de Diagnóstico e Investigación de la Enfermedad de Chagas, Ministerio de Salud y Acción Social, Buenos Aires, Argentina.
Búa
J
J
Aslund
L
L
Porcel
B
B
Segura
E L
EL
Henriksson
J
J
Orn
A
A
Pettersson
U
U
Ruiz
A M
AM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D017125:Genes, Protozoan; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000235:genetics*; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000235:genetics*; D014444:Tyrosine Transaminase / Q000235:genetics*
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D014444:Tyrosine Transaminase