Serine-O-phosphate has affinity for type IV, but not type I, metabotropic glutamate receptor.
L-serine-O-phosphate (L-SOP) has been reported to antagonize phosphoinositide (PI) hydrolysis in slices of rat brain as stimulated by agonists of the metabotropic glutamate receptor (mGluR). In the present study, the affinity of L-SOP was examined in a baby hamster kidney (BHK) cells expressing subtype mGluR1 or mGluR4 of the mGluR family. L-SOP or D-SOP (3 mM) did not inhibit PI-hydrolysis as stimulated by 10 microM glutamate in BHK cells expressing mGluR1. However, L-SOP was a potent agonist at the mGluR4 subtype (IC50 = 4.4 +/- 1.4 microM), which is negatively coupled to adenylate cyclase, while D-SOP was weakly active (IC50 = 1260 +/- 190 microM).
Thomsen
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Department of Receptor Neurochemistry, Novo Nordisk A/S, Måløv, Denmark.
Suzdak
P D
PD
D000818:Animals; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D006224:Cricetinae; D003470:Culture Media; D000242:Cyclic AMP / Q000096:biosynthesis; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006868:Hydrolysis; D007668:Kidney / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010768:Phosphoserine / Q000378:metabolism*; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism*; D013237:Stereoisomerism
D003470:Culture Media; D005971:Glutamates; D010716:Phosphatidylinositols; D018094:Receptors, Metabotropic Glutamate; D010768:Phosphoserine; D005576:Colforsin; D018698:Glutamic Acid; D000242:Cyclic AMP