Multiple elements in the c-fos protein-coding region facilitate mRNA deadenylation and decay by a mechanism coupled to translation.
The c-fos proto-oncogene transcript is one of the most labile mammalian mRNAs known. Rapid degradation of c-fos mRNA is mediated by both the c-fos protein-coding region and an AU-rich element in the 3'-untranslated region. Here we present evidence that the c-fos coding region contains multiple destabilizing elements that can function independently to facilitate both deadenylation and decay of mRNA. The ability of these coding region destabilizing elements to direct deadenylation and decay requires the assembly of ribosomes at the 5' end of this domain and, most likely, translation of the message.
Schiavi
S C
SC
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Wellington
C L
CL
Shyu
A B
AB
Chen
C Y
CY
Greenberg
M E
ME
Belasco
J G
JG
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D005091:Exons; D015870:Gene Expression; D016762:Genes, fos; D051379:Mice; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009843:Oligoribonucleotides; D010957:Plasmids; D014176:Protein Biosynthesis; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins; D015183:Restriction Mapping; D014162:Transfection
D009843:Oligoribonucleotides; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins