Evaluation of three assays for the determination of serum total sialic acid.
There is increasing clinical interest in the measurement of serum total sialic acid (TSA). There are a number of methods available but with little data suggesting the preferred technique. We evaluated three serum sialic acid assays: the Warren assay, the Svennerholm assay, and a commercially available enzymatic method. The analytical performances of the three assays were similar, except that the enzymatic assay had a greater analytical range than the other assays and better precision at low and high serum sialic acid concentrations. The enzymatic assay was readily automated for use on a Cobas Bio analyser but was substantially more expensive than the other two methods.
Crook
M
M
Department of Clinical Chemistry, Guy's Hospital, London, England.
Haq
M
M
Tutt
P
P
D002624:Chemistry, Clinical / Q000379:methods*; D003124:Colorimetry; D006801:Humans; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000097:blood*
D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid