title: A new 2D NMR method for measurement of JHH coupling constants.
abstract: A new 2D NMR pulse sequence for E.COSY-type measurement of J(HH) coupling constants is introduced. It exploits a heteronuclear spin, e.g., 13C, for displacement in the omega(1) frequency dimension via a large heteronuclear J coupling. The experiment is demonstrated by application to a heptapeptide at the natural abundance 13C level. It is suitable, for example, for measurement of 3J(HH) and 4J(HH) coupling constants in peptides and proteins.
authors.affiliation: Department of Chemistry, University of Copenhagen, Denmark.
authors.forename: M D
authors.initials: MD
authors.lastname: Sørensen
authors.forename: J J
authors.initials: JJ
authors.lastname: Led
authors.forename: O W
authors.initials: OW
authors.lastname: Sørensen
mesh_terms: D000595:Amino Acid Sequence; D007328:Insulin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry
chemical_list: D007328:Insulin; D010446:Peptide Fragments