Regulation of a hevein-like gene in Arabidopsis.
An Arabidopisis cDNA clone was isolated that encodes a protein similar to the antifungal chitin-binding protein hevein from rubber tree latex. This hevein-like (HEL) mRNA was inducible by either turnip crinkle virus infection or ethylene treatment. In addition, expression was moderately inducible by treatment with the resistance-inducing compounds salicylic acid and 2,6-dichlorisonicotinic acid. The 786-bp cDNA contains an open reading frame of 212 codons. The deduced amino acid sequence contains a putative signal sequence of 21 amino acids followed by a 43-amino-acid cysteine-rich lectin domain and a 129-amino-acid carboxy-terminal domain. The predicted protein is approximately 70% identical to hevein, to the wound-inducible WIN1 and WIN2 proteins from potato, and to PR-4, a pathogenesis-related protein from tobacco.
Potter
S
S
Department of Molecular Genetics, Ciba-Geigy Corporation, Research Triangle Park, NC 27709.
Uknes
S
S
Lawton
K
K
Winter
A M
AM
Chandler
D
D
DiMaio
J
J
Novitzky
R
R
Ward
E
E
Ryals
J
J
D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D005810:Multigene Family; D037121:Plant Lectins; D010940:Plant Proteins / Q000235:genetics*; D010942:Plant Viruses / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D037121:Plant Lectins; D010940:Plant Proteins; D012333:RNA, Messenger; C045875:hevein