Comparative study on the localization of adult Schistosoma mansoni worms in albino mice anesthetized with pentobarbital sodium, ether or chloroform.
The effect of anesthetic drugs on the localization of adult worms in albino mice was compared. The animals with 56 days of infection were anesthetized with pentobarbital sodium, ether or chloroform. Perfusion was carried out immediately after, recovering the worms and classifying them in relation to their localization on the liver or portal vein and the mesenteric veins. Our results showed that pentobarbital sodium produced a greater displacement of the worms to the liver (89%) than ether (76%) and chloroform (34%) did, when compared to the control group (22%). The difference between pentobarbital sodium and ether was significant (p < 0.05). We suggest that anesthetic drugs may not be used in studies on the distribution of adult worms in several hosts.
Machado e Silva
J R
JR
Department of Pathology and Laboratory Services, State University of Rio de Janeiro-UERJ, Brasil.
de Lima
M A
MA
Rodrigues e Silva
R
R
Maldonado Júnior
A
A
Presgrave
O A
OA
Rey
L
L
D000818:Animals; D002725:Chloroform / Q000494:pharmacology*; D004986:Ether / Q000494:pharmacology*; D008099:Liver / Q000469:parasitology; D008642:Mesenteric Veins / Q000469:parasitology; D051379:Mice; D010424:Pentobarbital / Q000494:pharmacology*; D011169:Portal Vein / Q000469:parasitology; D012550:Schistosoma mansoni / Q000187:drug effects*
D004986:Ether; D002725:Chloroform; D010424:Pentobarbital