Site-directed mutagenesis in hemoglobin: functional and structural role of the penultimate tyrosine in the alpha subunit.
The penultimate tyrosine in the hemoglobin subunit is considered to be one of the most important residues for the normal structure and function of hemoglobin. To elucidate the functional and structural role of the penultimate residue in the alpha-subunit, we prepared new artificial mutants; Hb Y140 alpha Q, in which Tyr-140 alpha is replaced by a nonaromatic residue, Gln, and Hb Y140 alpha F, which loses its hydrogen bond to Val-93 alpha by the substitution of Phe for Tyr. HB Y140 alpha Q exhibited a markedly increased oxygen affinity and almost completely diminished cooperativity, whereas Hb Y140 alpha F showed similar but less extensively impaired function, indicating that the aromatic residue at the penultimate position in the alpha-subunit contributes to the stabilization of the T-quaternary structure as does the corresponding residue in the beta-subunit. However, the deoxygenated forms of these mutants bear significant T-state character in their spectroscopic properties observed at high protein concentrations. The tetramer-dimer equilibrium data of the mutants suggested that a significant part of the functional alterations observed for dilute solution appears to result from partial dissociation into alpha beta dimers rather than direct destabilization of the T-quaternary structure in the deoxygenated form. Therefore, we can conclude that the penultimate tyrosine in the alpha-chain plays a key role not only in the stabilization of the T-state but also in the subunit assembly.(ABSTRACT TRUNCATED AT 250 WORDS)
Ishimori
K
K
Division of Molecular Engineering, Graduate School of Engineering, Kyoto University, Japan.
Hashimoto
M
M
Imai
K
K
Fushitani
K
K
Miyazaki
G
G
Morimoto
H
H
Wada
Y
Y
Morishima
I
I
D000494:Allosteric Regulation; D005914:Globins / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D016297:Mutagenesis, Site-Directed; D010108:Oxyhemoglobins / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D013329:Structure-Activity Relationship; D014443:Tyrosine / Q000737:chemistry
D046911:Macromolecular Substances; D010108:Oxyhemoglobins; D011994:Recombinant Proteins; D014443:Tyrosine; D005914:Globins