title: The corn inhibitor of blood coagulation factor XIIa. Crystallization and preliminary crystallographic analysis.
abstract: A 13.6 kDa protein from corn seeds is known to be a highly selective inhibitor of human blood coagulation Factor XIIa (or activated Hageman factor). We have crystallized this inhibitor at 23 degrees C and pH 7.5 from a solution of 30% polyethylene glycol 400, 0.2 M MgCl2, and 0.1 M Hepes. The crystals diffract to at least 2.1 A resolution. The space group is P4(2)2(1)2 with a = b = 57.15 A and c = 80.5 A. The crystals contain 51% solvent. Two heavy atom derivatives have been identified.
authors.affiliation: Department of Biochemistry, University of Washington, Seattle 98195.
authors.forename: L C
authors.initials: LC
authors.lastname: Pedersen
authors.forename: V C
authors.initials: VC
authors.lastname: Yee
authors.forename: G
authors.initials: G
authors.lastname: von Dassow
authors.forename: M
authors.initials: M
authors.lastname: Hazeghazam
authors.forename: G R
authors.initials: GR
authors.lastname: Reeck
authors.forename: R E
authors.initials: RE
authors.lastname: Stenkamp
authors.forename: D C
authors.initials: DC
authors.lastname: Teller
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D015956:Factor XIIa / Q000037:antagonists & inhibitors*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry; D003313:Zea mays
chemical_list: D011994:Recombinant Proteins; D015956:Factor XIIa