Non-invasive detection of Chlamydia trachomatis genital infections in asymptomatic males and females by enzyme immunoassay (Chlamydiazyme).
First voided urine, urethral and cervical swabs were collected from 300 asymptomatic inmates made up of 200 males and 100 females from prisons in Fiji. The enzyme immunoassay for detection of chlamydial antigen test was performed on the sediment of the urine samples. The prevalence of chlamydial urethritis in the study population of men as determined by culture of urethral swabs was 15%. Compared with the urethral cultures for chlamydia the urine EIA had a sensitivity of 86.6% and a specificity of 98.2%. In women the incidence of urogenital chlamydia infection was 18%. Using the results of the cervical swabs as a reference standard the urine EIA in the females showed a sensitivity of 61.1% with a specificity of 97.6%. Culturing urine sediments from both men and women showed low results (30.0% for men and 22.2% for women). The rapid enzyme immunoassay testing of male FVU sediment appears to be a reliable, non-traumatic and rapid method of diagnosing Chlamydia infection. The judicious use of this non-invasive method may help in detecting and treating men, thus reducing the rapid spread of the pathogen in the community.
Adjei
O
O
Fiji School of Medicine, Suva.
Lal
V
V
D000328:Adult; D000942:Antigens, Bacterial / Q000652:urine*; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000453:epidemiology; D002692:Chlamydia trachomatis / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D005831:Genital Diseases, Female / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D015995:Prevalence; D011329:Prisoners; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D014521:Urethra / Q000382:microbiology; D014526:Urethritis / Q000175:diagnosis* / Q000453:epidemiology
D000942:Antigens, Bacterial