Requirement for CD8 beta chain in positive selection of CD8-lineage T cells.
CD8 is either an alpha alpha homodimer or an alpha beta heterodimer, although most peripheral CD8-lineage T cells express only the CD8 alpha beta heterodimer. The physiological function of CD8 beta was elucidated with mice that were chimeric for the homozygous disruption of the CD8 beta gene. The CD8 beta-1- T cells developed normally to CD4+CD8+ stage, but did not efficiently differentiate further, which resulted in few peripheral CD8+ T cells. The number of peripheral CD8+ T cells was restored by transfer of an exogenous CD8 beta gene into CD8 beta-deficient T cells. Thus, CD8 beta is necessary for the maturation of CD8+ T cells.
Nakayama
K
K
Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, MO 63110.
Nakayama
K
K
Negishi
I
I
Kuida
K
K
Louie
M C
MC
Kanagawa
O
O
Nakauchi
H
H
Loh
D Y
DY
D000818:Animals; D015704:CD4 Antigens / Q000235:genetics; D016516:CD4-CD8 Ratio; D016827:CD8 Antigens / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D002678:Chimera; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D014162:Transfection
D015704:CD4 Antigens; D016827:CD8 Antigens; D015395:Histocompatibility Antigens Class I; D011948:Receptors, Antigen, T-Cell