[Methods of detection of P-fimbria in uropathogenic E. coli].
We characterized the surface properties of E. coli strains isolated from urine of children with urinary tract infections. The PF test and haemagglutination reaction were used for detection of P-fimbriae. P-fimbriae were detected in 30-54% of the pyelonephritic strains. All these strains expressed P-fimbriae in vitro. Pap genes were determined by a DNA probe in 85% of the strains isolated from pyelonephritis. These strains showed diffuse and localized adherence to tissue culture cells without any association to the expression of P-fimbriae on agar medium.
Bartková
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Ustav preventívnej a klinickej medicíny, Bratislava.
Ciznár
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I
D001422:Bacterial Adhesion; D002648:Child; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D010861:Fimbriae, Bacterial; D006386:Hemagglutination Tests; D006801:Humans; D014552:Urinary Tract Infections / Q000382:microbiology*
Metodické aspekty dôkazu P-fimbrií uropatogénnych E. coli.