Effect of serum on differentiation of porcine adipose stromal-vascular cells in primary culture.
1. Serum-containing media without additional hormones failed to support the differentiation of porcine SV cells while hormone-supplemented serum-free medium (ITTC) stimulated differentiation as indicated by higher GPDH specific activity. 2. All sera tested stimulated proliferation and inhibited porcine SV cell differentiation to some degree; fetal calf and rat sera were more inhibitory than calf and pig sera. 3. Morphologically, in serum-free medium porcine SV cells developed into individual adipocytes while in all serum-containing media they formed fat cell clusters. 4. Insulin and hydrocortisone in serum-free medium were important factors in SV cell differentiation while transferrin, T3, and fibroblast growth factor (FGF) were less important.
Suryawan
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A
Department of Animal Sciences, Oregon State University, Corvallis 97331-6702.
Hu
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CY
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001769:Blood; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005346:Fibroblast Growth Factors / Q000502:physiology; D007328:Insulin / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology; D013552:Swine; D014168:Transferrin / Q000502:physiology
D016895:Culture Media, Serum-Free; D007328:Insulin; D014168:Transferrin; D005346:Fibroblast Growth Factors