title: Endolymphatic sac blood flow versus cochlear blood flow following intravenous administration of isosorbide in guinea pigs.
abstract: Endolymphatic sac (ES) blood flow (ESBF) and cochlear blood flow (CBF) were measured in different groups of guinea pigs by laser-Doppler flowmetry (Advance Laser Flowmeter, Model ALF 2100) after the intravenous administration of 70% isosorbide (1.6 ml/kg). The measurements were made under general anesthesia with intraperitoneal pentobarbital sodium. Respiration was controlled by a respirator after tracheotomy, and blood pressure was monitored through the femoral artery (Gould Statham P23 ID Pressure Transducer). For ESBF measurements, a probe was placed on the right ES after entering the posterior cranial fossa via the dorsal approach. For CBF measurements, a probe was placed on the basal turn of the right cochlea via the ventral approach. Isosorbide was administered intravenously through the jugular vein for 60 s. Both ESBF and CBF increased immediately after administration, reached a peak within 3-6 min and decreased gradually to their initial baseline levels in 11-15 min. Both blood flow changes almost always corresponded to systemic blood pressure changes, although a slight delay was observed in blood pressure compared to the blood flow. The magnitude of the CBF response tended to be greater than that of the ESBF response (p < 0.1). This may result from the anatomical differences in the two blood supplies, i.e., from the vertebral artery (CBF) and the external carotid artery (ESBF).
authors.affiliation: Department of Otolaryngology, Shiga University of Medical Science, Otsu, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yazawa
authors.forename: M
authors.initials: M
authors.lastname: Kitahara
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002342:Carotid Artery, External / Q000033:anatomy & histology; D003051:Cochlea / Q000098:blood supply* / Q000187:drug effects*; D004712:Endolymphatic Sac / Q000098:blood supply* / Q000187:drug effects*; D006168:Guinea Pigs; D007262:Infusions, Intravenous; D007547:Isosorbide / Q000008:administration & dosage / Q000494:pharmacology*; D007601:Jugular Veins; D017078:Laser-Doppler Flowmetry; D012039:Regional Blood Flow / Q000187:drug effects; D013997:Time Factors; D014711:Vertebral Artery / Q000033:anatomy & histology
chemical_list: D007547:Isosorbide