title: Targeting of c-myc and beta-globin coding sequences to cytoskeletal-bound polysomes by c-myc 3' untranslated region.
abstract: The influence of the 3' untranslated region on mRNA localization was investigated by measuring the distribution of myc, beta-globin and hybrid myc-globin mRNAs between free, cytoskeletal-bound and membrane-bound polysomes in cells transfected with either control or chimeric gene constructs. c-myc sequences and beta-globin-coding sequences linked to the myc 3' untranslated region were present at greatest enrichment in cytoskeletal-bound polysomes. beta-Globin mRNA and myc-coding sequences linked to the beta-globin 3' untranslated region were recovered largely in the free polysomes. In situ hybridization confirmed that replacement of the c-myc 3' untranslated region by that of globin caused a relocalization of the mRNA. The results suggest that mRNA localization in differentiated eukaryotic cells depends on a mechanism that involves directional information in the 3' untranslated region of mRNAs.
authors.affiliation: Division of Biochemical Sciences, Rowett Research Institute, Aberdeen, Scotland, U.K.
authors.forename: J
authors.initials: J
authors.lastname: Hesketh
authors.forename: G
authors.initials: G
authors.lastname: Campbell
authors.forename: M
authors.initials: M
authors.lastname: Piechaczyk
authors.forename: J M
authors.initials: JM
authors.lastname: Blanchard
mesh_terms: D000818:Animals; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism*; D016259:Genes, myc; D005914:Globins / Q000235:genetics*; D017403:In Situ Hybridization; D007438:Introns; D051379:Mice; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits
chemical_list: D012333:RNA, Messenger; D005914:Globins