Analysis of the cos region of the Lactococcus lactis bacteriophage sk1.
The location, structure and nature of the cos site of the Lactococcus lactis bacteriophage sk1 was determined using a Taq DNA polymerase runoff sequencing technique. The cos site contains a single-stranded 3' overhang of 11 nucleotides. The region surrounding cos contains several features which may be involved in the binding and catalytic action of a phage terminase. These include four putative terminase-binding sites which show some homology to lambda R-sites, an 11-bp direct repeat, a 10-bp inverted repeat, a string of eight consecutive C residues and six copies of the pentanucleotide, AATCT. The spacing between adjacent copies of the pentanucleotides would place them on the same side of the DNA helix.
Chandry
P S
PS
Commonwealth Scientific and Industrial Research Organization, Division of Food Science and Technology, Highett, Victoria, Australia.
Moore
S C
SC
Davidson
B E
BE
Hillier
A J
AJ
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D019914:Taq Polymerase
D004279:DNA, Viral; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase; D004706:Endodeoxyribonucleases; C030894:terminase