Crystallization and preliminary X-ray analysis of the auto-inhibited twitchin kinase.
An auto-inhibited fragment of twitchin kinase (residues 5890 to 6262) has been crystallized by vapor diffusion techniques using polyethylene glycol 4000 as the precipitant at pH 7.25 to 7.5 at 4 degrees C. We have found that MgSO4 and glycerol were essential for large crystal growth. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit cell dimensions of a = 144.1 A, b = 168.3 A and c = 60.6 A. They are suitable for X-ray analysis and diffract to a resolution of at least 2.8 A.
Hu
S H
SH
St Vincent's Institute of Medical Research, Fitzroy, Victoria, Australia.
Lei
J Y
JY
Wilce
M C
MC
Valenzuela
M R
MR
Benian
G M
GM
Parker
M W
MW
Kemp
B E
BE
D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology; D029742:Caenorhabditis elegans Proteins; D002148:Calmodulin-Binding Proteins; D002645:Chickens; D003460:Crystallization; D018360:Crystallography, X-Ray; D015801:Helminth Proteins; D015394:Molecular Structure; D009124:Muscle Proteins; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000737:chemistry*
D029742:Caenorhabditis elegans Proteins; D002148:Calmodulin-Binding Proteins; D015801:Helminth Proteins; D009124:Muscle Proteins; D010446:Peptide Fragments; D047428:Protein Kinase Inhibitors; C489233:unc-22 protein, C elegans; D011494:Protein Kinases; D009219:Myosin-Light-Chain Kinase