Latex antigens induce IgE and eosinophils in mice.
Hypersensitivity to latex proteins has been reported with increasing frequency in recent years. Elevated levels of latex specific IgE have been detected in the majority of these patients. Severe anaphylaxis and death resulting from latex exposure has also been reported. Nevertheless, the immune mechanism of latex allergy is not fully understood. In this report, we describe a model of latex allergy developed in mice exposed to latex proteins. Animals exposed to latex proteins demonstrated enhanced levels of total IgE, peripheral blood and lung eosinophilia, and elevated levels of serum IL-4 and IL-5. mRNA transcripts of IL-4 and IL-5, but not IFN-gamma, could be demonstrated in spleen lymphocytes. Antibodies to latex belonging to all IgG subclasses were detected in the sera of mice exposed to latex antigens. The histology of the lung showed non-necrotizing granulomas and extensive interstitial chronic inflammatory infiltrates, particularly around bronchioles and small blood vessels. Although this model of latex allergy demonstrates a heterogeneous immunological response, the CD4-positive Th2 cell-mediated response predominated.
Kurup
V P
VP
Department of Medicine, Medical College of Wisconsin, Milwaukee.
Kumar
A
A
Choi
H
H
Murali
P S
PS
Resnick
A
A
Kelly
K J
KJ
Fink
J N
JN
D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines / Q000235:genetics; D004804:Eosinophils / Q000276:immunology*; D015870:Gene Expression; D006967:Hypersensitivity / Q000276:immunology* / Q000473:pathology; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000235:genetics; D015847:Interleukin-4 / Q000097:blood / Q000235:genetics; D015848:Interleukin-5 / Q000097:blood / Q000235:genetics; D007840:Latex; D008168:Lung / Q000473:pathology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics
D000941:Antigens; D016207:Cytokines; D015848:Interleukin-5; D007840:Latex; D012333:RNA, Messenger; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma