Enhanced yields of gamma interferon in prolactin treated human peripheral blood mononuclear cells.
Prolactin is a peptide hormone with effects on a number of target organs including the immune system. It has been shown that animals rendered hypoprolactinemic have impaired delayed hypersensitivity, impaired macrophage activation and altered secretion of gamma interferon (IFN). Using peripheral blood mononuclear cells (PBMC) and inducing the cells to produce gamma IFN with a range of inducers, we have studied the effects of a number of hormones on IFN production. Using cells from normal donors, we have found that prolactin in concentrations of 10(-8) M or greater, can significantly enhance the production of gamma IFN. The effect was dose related and was observed when lectins (PHA and Con A), but not anti CD3 antibodies, ionophones, or IL-2 were used to induce the cells. The presence of prolactin in concentrations above that encountered in the fetal bovine serum used to incubate the cells resulted in a doubling or more of the IFN produced. The tests were performed on 30 occasions with cells drawn from 21 individuals. On all but three occasions, yield enhancement was observed in the presence of prolactin. The mechanism of the effect was investigated, and genistein, a tyrosine kinase inhibitor, was found to abort the influence of prolactin on gamma IFN production. These studies indicate prolactin in physiological concentrations can enhance the production of gamma IFN from cells from normal donors.
Cesario
T C
TC
Department of Medicine, University of California, Irvine, Medical Center, Orange 92668.
Yousefi
S
S
Carandang
G
G
Sadati
N
N
Le
J
J
Vaziri
N
N
D000906:Antibodies / Q000494:pharmacology; D017252:CD3 Complex / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D003208:Concanavalin A; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000097:blood; D007376:Interleukin-2 / Q000494:pharmacology; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010835:Phytohemagglutinins; D011388:Prolactin / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000906:Antibodies; D017252:CD3 Complex; D007376:Interleukin-2; D010835:Phytohemagglutinins; D003208:Concanavalin A; D015759:Ionomycin; D007371:Interferon-gamma; D011388:Prolactin; D013755:Tetradecanoylphorbol Acetate