Isolation and identification of a cardioactive peptide from Tenebrio molitor and Spodoptera eridania.
We isolated several cardioactive peptides from extracts of whole heads of the mealworm, Tenebrio molitor, and the southern armyworm, Spodoptera eridania, using a semi-isolated heart of Manduca sexta for bioassay. We have now isolated from each species the peptide with the strongest effect on rate of contraction of the heart. The peptides were identified using micro Edman sequencing and mass spectrometric methods. This cardioactive peptide has the same primary structure from both species: Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2, a cyclic nonapeptide which is identical to crustacean cardioactive peptide (CCAP) originally isolated from the shore crab, Carcinus maenas, and subsequently isolated from Locusta migratoria and Manduca sexta. This is additional evidence that CCAP has widespread occurrence in arthropoda.
Furuya
K
K
Department of Biochemistry, University of Nevada, Reno.
Liao
S
S
Reynolds
S E
SE
Ota
R B
RB
Hackett
M
M
Schooley
D A
DA
D000595:Amino Acid Sequence; D000818:Animals; D002317:Cardiovascular Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002853:Chromatography, Liquid; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011487:Protein Conformation; D013712:Tenebrio / Q000737:chemistry*
D002317:Cardiovascular Agents; D010456:Peptides, Cyclic