Demineralization of bone by ammonium chloride for the pyroantimonate method.
Rat bones were fixed by the pyroantimonate method and demineralized by treatment with ammonium chloride (NH4Cl). Bones from very young rats (neonatal and 2-week-old rats) that had been treated with NH4Cl were easy to cut into ultrathin sections. However, bones of 8-week-old rats needed to be demineralized for a longer time and were not very easy to cut. The extent of demineralization was measured by scintillation counting. After demineralization, precipitates of calcium pyroantimonate (Ca-pyroantimonate) were well retained and the fine structure of the tissue was well preserved. In addition, the distribution of Ca-pyroantimonate in the demineralized bone was similar to that in undecalcified specimens. This demineralization method is used in combination with the pyroantimonate method.
Kawamata
S
S
Department of Anatomy, Toyama Medical and Pharmaceutical University, Japan.
D000643:Ammonium Chloride; D000818:Animals; D000831:Animals, Newborn; D000965:Antimony; D001842:Bone and Bones / Q000737:chemistry* / Q000648:ultrastructure*; D002118:Calcium / Q000302:isolation & purification; D005260:Female; D005404:Fixatives; D006652:Histological Techniques; D008297:Male; D008903:Minerals / Q000302:isolation & purification*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005404:Fixatives; D008903:Minerals; D000643:Ammonium Chloride; C009133:pyroantimonate; D000965:Antimony; D002118:Calcium