Cloning and expression of a human glutamate transporter.
A complementary DNA clone was isolated from human brain that encodes a glutamate transporter. Injection of RNA transcribed from this cDNA into Xenopus oocytes resulted in expression of a transport activity with a high affinity for glutamate (Km = 78.4 microM) and a dependency on external Na+. The cDNA sequence predicts a protein of 542 amino acids that is highly homologous to the congeneric proteins from rat and mouse brains. RNA blotting analyses revealed the expression both in the brain and in peripheral tissues.
Kawakami
H
H
Third Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Tanaka
K
K
Nakayama
T
T
Inoue
K
K
Nakamura
S
S
D000595:Amino Acid Sequence; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport / Q000187:drug effects; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D006023:Glycoproteins / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D014018:Tissue Distribution; D014982:Xenopus laevis
D027322:Amino Acid Transport System X-AG; D018076:DNA, Complementary; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger