Intramolecular recombination in polyomavirus DNA is controlled by promoter elements.
We show here that intramolecular homologous recombination in polyomavirus (Py) DNA depends upon discrete sequence elements of the viral regulatory region which are believed to regulate transcription initiation and exert little or no cis-control over replication. Either deleting the viral early promoter (EP) or inverting the viral late promoter (LP) strongly impairs viral DNA recombination under conditions allowing viral DNA replication to proceed undisturbed. These findings suggest that bi-directional transcription proceeding from the intergenic region favors intramolecular recombination.
Nault
C
C
Department of Microbiology, Faculty of Medicine, Université de Sherbrooke, Québec, Canada.
Veilleux
S
S
Delbecchi
L
L
Bourgaux-Ramoisy
D
D
Bourgaux
P
P
D001483:Base Sequence; D002474:Cell-Free System; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
D017931:DNA Primers; D004279:DNA, Viral