Magnetization transfer affects the proton creatine/phosphocreatine signal intensity: in vivo demonstration in the rat brain.
The effect of off-resonance preirradiation on proton spectra acquired from the healthy rat brain at 4.7 T is examined using a PRESS sequence. The creatine/phosphocreatine (Cr/PCr) signal at 3.0 ppm decreases in signal intensity for offset frequencies between +/- 10 kHz. This cannot be explained by RF bleedover, but is attributed to magnetization transfer between two pools of Cr/PCr, one with a long T2 relaxation time giving the observed NMR signal, the other corresponding to a broad resonance line being completely or partially saturated by off-resonance preirradiation. Possible interpretations of these results are discussed.
Dreher
W
W
Universität Bremen, Fachbereich Chemie/Biologie, Bremen, Federal Republic of Germany.
Norris
D G
DG
Leibfritz
D
D
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000032:analysis; D001923:Brain Chemistry; D003401:Creatine / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D010725:Phosphocreatine / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar
D010725:Phosphocreatine; D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine