Genes for members of the GATA-binding protein family (GATA-GT1 and GATA-GT2) together with H+/K(+)-ATPase are specifically transcribed in gastric parietal cells.
mRNAs for novel DNA-binding proteins (GATA-GT1 and GATA-GT2) recognizing the (G/C)PuPu(G/C)NGAT(A/T)PuPy sequence and H+/K(+)-ATPase (proton pump) alpha subunit were detected in parietal cells of the rat gastric body mucosa by in situ hybridization. These results suggest that GATA-GT1 and GATA-GT2 together with H+/K(+)-ATPase are transcribed specifically in gastric parietal cells and that the two DNA-binding proteins may have important roles in cell specific gene regulation. Furthermore, we could detect parietal cells in different states of gene expression.
Mushiake
S
S
Department of Pediatrics, Osaka University Medical School, Japan.
Etani
Y
Y
Shimada
S
S
Tohyama
M
M
Hasebe
M
M
Futai
M
M
Maeda
M
M
D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics*; D050991:GATA4 Transcription Factor; D050993:GATA6 Transcription Factor; D017403:In Situ Hybridization; D005810:Multigene Family; D010295:Parietal Cells, Gastric / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D050991:GATA4 Transcription Factor; D050993:GATA6 Transcription Factor; C084365:Gata4 protein, rat; C084364:Gata6 protein, rat; D014157:Transcription Factors; D000254:Sodium-Potassium-Exchanging ATPase