Effect of acetylcholine infusion on adrenal vasculature and catecholamine secretion.
To evaluate effects of cholinergic receptor stimulation on regional adrenal blood flow (Q, radiolabeled microspheres) and catecholamine secretion, acetylcholine (ACh) was infused into pentobarbital-anesthetized, ventilated dogs. Unilateral adrenal denervation and placement of lumboadrenal catheters preceded intra-aortic infusion of 1) ACh alone (n = 6), 2) ACh plus hexamethonium (Hex) 20 mg/kg (n = 6), or 3) ACh plus atropine (Atr) (0.5 mg/kg) and Hex. ACh alone and in combination with Hex elicited similar dose-related (2, 20, and 100 mumol/min) increases in catecholamine secretion (181 +/- 61 to 1,055 +/- 229, 31,644 +/- 9,411, and 179,181 +/- 69,659 ng.min-1 x g medulla-1), whereas Hex and Atr together inhibited ACh-induced secretion by 95%. ACh caused marked medullary vasodilation (0.71 +/- 0.05 to 0.14 +/- 0.03 mmHg.ml-1 x min.100 g) in all three groups. To determine whether medullary vasodilation was due to incomplete muscarinic blockade, Hex-pretreated animals (n = 4) received ACh (100 mumol/min) and three increasing doses of Atr (0.5, 5, and 25 mg/kg). Catecholamine secretion was inhibited by all doses of Atr; however, vasodilation was blocked only by the two higher doses of Atr. These data suggest possible different mechanisms of muscarinic receptor-mediated catecholamine secretion and vasodilation.
Tobin
J R
JR
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland 21287.
Breslow
M J
MJ
Traystman
R J
RJ
D000109:Acetylcholine / Q000494:pharmacology*; D000311:Adrenal Glands / Q000098:blood supply* / Q000378:metabolism*; D000818:Animals; D001011:Aorta; D001285:Atropine / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D001808:Blood Vessels / Q000187:drug effects; D002395:Catecholamines / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D007267:Injections; D012039:Regional Blood Flow / Q000187:drug effects; D014655:Vascular Resistance / Q000187:drug effects; D014664:Vasodilation
D002395:Catecholamines; D004338:Drug Combinations; D006584:Hexamethonium Compounds; D018738:Hexamethonium; D001285:Atropine; D000109:Acetylcholine