Crystallization and preliminary X-ray analysis of apo-S100 beta and S100 beta with Ca2+.
Crystals of a bovine neurotrophic factor S100 beta with and without Ca2+ bound has been crystallized by vapor diffusion from solutions of polyethylene glycol. The crystals of S100 beta with Ca2+ bound were monoclinic and belong to space group P21 with unit cell dimensions a = 36.2 A, b = 55.63 A, c = 48.39 A and beta = 111.77 degrees. The asymmetric unit contains two molecules (one dimer) which exhibit an approximate 222 point symmetry. The crystals of apo-S100 beta were tetragonal and belong to space group P4(1) with unit cell dimensions a = b = 56.04 A, c = 111.7 A. The asymmetric unit contains four molecules (two dimers) which exhibit 422 point symmetry. Both these crystal forms diffract to greater than 1.9 A resolution.
St Charles
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Department of Pharmacology, Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107.
Kumar
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D000818:Animals; D001059:Apoproteins / Q000737:chemistry / Q000302:isolation & purification; D001923:Brain Chemistry; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D046911:Macromolecular Substances; D009414:Nerve Growth Factors / Q000737:chemistry* / Q000302:isolation & purification; D011092:Polyethylene Glycols; D011485:Protein Binding; D011487:Protein Conformation; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D012984:Software
D001059:Apoproteins; D002135:Calcium-Binding Proteins; D046911:Macromolecular Substances; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D011092:Polyethylene Glycols; D002118:Calcium