Double-strand DNA conformation polymorphisms as a source of highly polymorphic genetic markers.
The molecular basis for several apparent restriction fragment length polymorphisms of the porcine growth hormone gene was examined through DNA sequence analysis. Electrophoretic and sequence analysis suggest polymorphisms result from 1-3 base substitutions that affect double-strand DNA conformation and electrophoretic mobility. Two allelic forms of the porcine growth hormone 5' flank and four allelic forms of the second exon/intron region were identified. A marker system was developed which combined conformation polymorphisms with HaeII and DdeI RFLPs. Using this system, nine haplotypes were observed in samples from three US swine breeds. The data presented suggest that double-strand DNA conformation can be exploited in base substitution detection and development of highly polymorphic genetic marker systems.
Kirkpatrick
B W
BW
Department of Meat and Animal Science, University of Wisconsin, Madison 53706.
Huff
B M
BM
Casas-Carrillo
E
E
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005787:Gene Frequency; D008040:Genetic Linkage; D005819:Genetic Markers; D013006:Growth Hormone / Q000235:genetics*; D006239:Haplotypes; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010375:Pedigree; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000235:genetics*
D005819:Genetic Markers; D013006:Growth Hormone; D004247:DNA