title: Production of minor lymphocyte stimulatory-1a antigen from activated CD4+ or CD8+ T cells.
abstract: Minor lymphocyte stimulatory (Mls) Ag are super Ag that stimulate a high proportion of T cells of a specific TCR V beta family. One of the super Ag, Mls-1a, which is recognized mainly by TCR V beta 6+ and V beta 8.1+ T cells, has recently been linked to the response to product of the open reading frame in 3'-long terminal repeat of endogenous mammary tumor virus, MTV-7. It is quite certain that B cells are able to produce and also to present the Mls-1a Ag. However, it remains to be determined whether other cell types, especially T cells, produce Mls-1a Ag. In this study using highly purified T cell subpopulations, capacity to produce Mls-1a Ag was analyzed by calculating the proportion of Mls-1a reactive V beta 6+ or V beta 8.1+ T cells in responding cell populations. We found that nonstimulated CD8+ T cells produced a low amount of Mls-1a Ag, and the capacity to do so was considerably increased by stimulation with immobilized anti-TCR mAb. By contrast, nonstimulated CD4+ T cells did not produce Mls-1a Ag at all. Even when CD4+ T cells were activated via TCR signaling with immobilized anti-TCR mAb, CD4+ T cells did not produce Mls-1a Ag. However, CD4+ T cells primed with conventional Ag in vivo produced Mls-1a Ag on restimulation with that specific Ag in vitro. These findings indicate that not only CD8+ T cells but also CD4+ T cells can produce Mls-1a Ag on appropriate stimulation, although different mechanisms for Mls-1a production may operate between the CD4+ and CD8+ T cells.
authors.affiliation: Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Arase-Fukushi
authors.forename: H
authors.initials: H
authors.lastname: Arase
authors.forename: K
authors.initials: K
authors.lastname: Ogasawara
authors.forename: R A
authors.initials: RA
authors.lastname: Good
authors.forename: K
authors.initials: K
authors.lastname: Onoé
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D015704:CD4 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016827:CD8 Antigens / Q000032:analysis*; D002478:Cells, Cultured; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008806:Mice, Inbred AKR; D016747:Minor Lymphocyte Stimulatory Antigens / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016827:CD8 Antigens; D007376:Interleukin-2; D016747:Minor Lymphocyte Stimulatory Antigens; D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta