Asymmetric fusion between protoplasts of tomato (Lycopersicon esculentum Mill.) and gamma-irradiated protoplasts of potato (Solanum tuberosum L.): the effects of gamma irradiation.
This paper describes the aggregation of nuclei in heterokaryons of tomato and unirradiated or irradiated potato protoplasts and the effects of gamma irradiation of potato and tomato protoplasts on single- and double-stranded DNA fragmentation, DNA repair and DNA synthesis as revealed by alkaline and pulsed field gel electrophoresis and an immunocytochemical technique. The prospects for obtaining highly asymmetric somatic hybrids of tomato and gamma-irradiated potato are discussed.
Schoenmakers
H C
HC
Department of Genetics, Wageningen Agricultural University, The Netherlands.
van der Meulen-Muisers
J J
JJ
Koornneef
M
M
D002459:Cell Fusion / Q000528:radiation effects*; D002467:Cell Nucleus; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000096:biosynthesis / Q000528:radiation effects; D005720:Gamma Rays; D011523:Protoplasts / Q000528:radiation effects*; D011198:Solanum tuberosum / Q000166:cytology* / Q000528:radiation effects; D014675:Vegetables / Q000166:cytology* / Q000528:radiation effects
D004277:DNA, Single-Stranded; D004247:DNA