title: [Densitometric determination of dithiadenoxide in blood].
abstract: The present paper describes a method of dithiadenoxide determination in blood plasma. Dithiadenoxide was reduced to dithiaden with zinc dust in acidified plasma. The solution was alkalified and dithiaden extracted into n-hexane and isolated on a thin layer of silica gel using the system chloroform--n-heptane-methanol (60:22:18). After spraying the plate with a solution of m-nitrophenyldiazoniumfluoroborate in acid medium, dithiaden stains were evaluated densitometrically in the absorption maximum of the reaction product at 565 nm (RF = 0.37). Detection limit of dithiadenoxide in the proposed method is 0.004 microgram in an applied amount.
authors.affiliation: Výzkumný ústav pro farmacii a biochemii.
authors.forename: M
authors.initials: M
authors.lastname: Peterková
authors.forename: O
authors.initials: O
authors.lastname: Matousová
mesh_terms: D001582:Benzothiepins / Q000097:blood*; D002845:Chromatography / Q000379:methods*; D006634:Histamine H1 Antagonists / Q000097:blood; D006801:Humans
chemical_list: D001582:Benzothiepins; D006634:Histamine H1 Antagonists; C083350:dithiadenoxide; C100170:dithiadene
vernacular_title: Densitometrické stanovení dithiadenoxidu v krevní plazmĕ.