Analysis of interleukin-2-activated killer cells of rhesus monkeys: striking resemblance to the human system.
We have found numerous and exquisite homologies between the interleukin-2 (IL-2)-activated killing systems of rhesus monkeys and humans. Lymphocytes with high oncolytic and proliferative activity were generated from peripheral blood, spleen, and bone marrow of monkeys after culture with IL-2. The distribution of lymphocyte subsets in IL-2 cultures closely paralleled that seen in humans, including a decrease in CD4+ and increase in CD8+, CD38+, and CD25+ lymphocytes and an increase in density of CD2 molecules. We also describe three distinct subsets of monkey lymphocytes, CD16+,56-, CD16+,56+"dim", and CD16-,56+"bright", and show that the CD56+"bright" subset is substantially increased (to as high as 79%) after IL-2 activation. Furthermore, as in humans, the cells with oncolytic activity were characterized as CD56+, CD16+/-, and CD8+. This strong homology with humans indicates that the rhesus monkey may be a valuable preclinical model for evaluation of therapeutically relevant biological response modifiers.
Savary
C A
CA
Department of Surgical Oncology, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
Lotzová
E
E
Jackson
H J
HJ
Jardine
J H
JH
Ang
K K
KK
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001853:Bone Marrow / Q000276:immunology; D018801:CD2 Antigens; D002455:Cell Division; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005260:Female; D015186:Hominidae / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects / Q000276:immunology*; D007700:Kinetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D016131:Lymphocyte Subsets / Q000276:immunology*; D016393:Lymphoma, B-Cell; D008253:Macaca mulatta / Q000276:immunology*; D010051:Ovarian Neoplasms; D017452:Receptors, IgG / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D007376:Interleukin-2; D017452:Receptors, IgG; D011971:Receptors, Immunologic