[Analysis of human chromosome 21q: isolation of linking clones and generation of STSs, and construction of P1 phage library].
To clarify the structure of human chromosome 21q, we isolated many Sequence-Tagged NotI and SfiI linking clones. For further analysis, we constructed a P1 phage library specific for this chromosome. The library presently consists of 1516 clones, amounting to 2 equivalents of this chromosome. For rapid clone identification, we constructed a 3-dimensional library containing DNA from P1 clones, and a PCR-based screening approach was used to isolate 38 positives from 50 sequence-tagged sites specific for this chromosome. And also by this PCR-based chromosome walking procedure using STSs from sequences from insert-end of P1 clones, we constructed a contig spanning the entirety of the human beta-amyloid precursor protein gene consisted of eight P1 clones and a contig consisted of nine P1 clones on D21S8 region.
Tanahashi
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Laboratory of Molecular Medicine, University of Tokyo.
D000544:Alzheimer Disease / Q000235:genetics; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D017101:Bacteriophage P1 / Q000235:genetics*; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004247:DNA; D015723:Gene Library; D008040:Genetic Linkage; D016045:Human Genome Project; D006801:Humans; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
D016564:Amyloid beta-Protein Precursor; D004247:DNA