Invasiveness of Shigella flexneri in poliovirus infected HT-29 cells.
In this paper we report the effect in HT-29 cells of mixed infections with poliovirus type 1 and Shigella flexneri serotype 5 strain M90T and derivative strains differing in adhesive as well as invasive properties. HT-29 epithelial intestinal cells derived from a human colon adenocarcinoma are a good model for coinfection studies because they are susceptible both to poliovirus replication and to S. flexneri invasion. The results show that 48 h after infection by poliovirus the invasiveness of wild-type S. flexneri M90T and of its adherent and invasive derivative M90T (pIL22) is increased. However, the alterations produced in HT-29 cells at the later stage of poliovirus infection are not sufficient to promote the internalization of simply non invasive, or non invasive but adherent, isogenic derivative bacterial strains.
Seganti
L
L
Istituto Pasteur-Fondazione Cenci Bolognetti, Università La Sapienza, Roma, Italy.
Conte
M P
MP
Longhi
C
C
Marchetti
M
M
Nicoletti
M
M
Orsi
N
N
D001422:Bacterial Adhesion; D002463:Cell Membrane Permeability; D006801:Humans; D007700:Kinetics; D017955:Poliovirus / Q000254:growth & development*; D012763:Shigella flexneri / Q000472:pathogenicity*; D014407:Tumor Cells, Cultured / Q000382:microbiology; D014774:Virulence