title: Amino acid sequence of beta-microseminoprotein from porcine seminal plasma.
abstract: beta-Microseminoprotein is a small mucus-associated protein of unknown function, previously characterized in man and the ape. The corresponding porcine protein was identified in tracheal mucus and seminal plasma by its cross-reaction with antibodies raised against the human protein. The porcine protein was isolated from seminal plasma, and its complete amino acid sequence determined. It is a single-chain protein of 91 amino acids with a molecular mass of 10,068 Da. Compared to primate beta-microseminoprotein, the porcine protein has a blocked N-terminus and a deletion of three amino acids in the N-terminal region, but otherwise manifests similarity in amino acid sequence including conservation of 10 cysteine residues. A proportion of individual pigs have two forms of the protein, differing in charge but not in size. The characterization of beta-microseminoprotein in a species suited for experiments should help determine the function of this protein.
authors.affiliation: Department of Clinical Chemistry, University of Lund, Malmö General Hospital, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Fernlund
authors.forename: L B
authors.initials: LB
authors.lastname: Granberg
authors.forename: P
authors.initials: P
authors.lastname: Roepstorff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000378:metabolism; D013552:Swine; D014357:Trypsin / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin