title: [Possibilities of ovum pickup in cattle].
abstract: Cattle breeding has a new reproduction technique in addition to artificial insemination and embryo transfer. It is the collection of ova from living animal by means of ultrasound guided follicle aspiration (ovum pick-up), followed by embryo-production in vitro. Follicles larger than 2 mm were punctured and the ova were collected twice weekly during 3 months. In total 1677 ova were collected from ten cows; 1342 (80%) were used for in vitro maturation, fertilization, and embryoculture. All ova were fertilized with semen from one bull, and 218 transferable embryos were produced. Calculated on a year basis, this would amount to 87 embryos per animal, with an intra-animal variation between 28 and 132. This new technique may replace MOET (Multiple Ovulation and Embryo Transfer; yearly average of 25 transferable embryos per animal), if the embryo-production via OPU can be performed with semen from any selected bull.
authors.affiliation: Embrytec B.V., Zeist.
authors.forename: M W
authors.initials: MW
authors.lastname: Roelofsen-Vendrig
authors.forename: R
authors.initials: R
authors.lastname: Boni
authors.forename: Y A
authors.initials: YA
authors.lastname: Wurth
authors.forename: M C
authors.initials: MC
authors.lastname: Pieterse
authors.forename: T A
authors.initials: TA
authors.lastname: Kruip
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D006080:Ovarian Follicle; D010063:Ovum; D011677:Punctures / Q000662:veterinary; D012099:Reproductive Techniques / Q000662:veterinary*
vernacular_title: Mogelijkheden van ovum pick-up bij het rund.