title: Receptors for C-type natriuretic peptide in cultured rat glial cells.
abstract: To characterize sites of action of C-type natriuretic peptide (CNP) in the glial cells, the effect of CNP on cGMP accumulation and the binding of [125I]CNP in rat astrocyte RCR-1 cells were studied. CNP stimulated cGMP accumulation in the cells from 10(-9) M in a dose-dependent manner, but ANP (atrial natriuretic peptide) had a negligible effect on cGMP accumulation in the cells. [125I]CNP was bound to the cells and its Kd value was 2 orders of magnitude lower than that of the ED50 value for stimulation of cGMP accumulation in the cells. Not only CNP but also ANP displaced [125I]CNP binding to the cells. These results suggest that RCR-1 cells have a B-receptor which contains a guanylate cyclase domain and is preferentially activated by CNP, and that they also have a C-receptor which does not contain a guanylate cyclase domain that reacts with both ANP and CNP.
authors.affiliation: Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Mizuki
authors.forename: M
authors.initials: M
authors.lastname: Tsutsui
authors.forename: K
authors.initials: K
authors.lastname: Minami
authors.forename: N
authors.initials: N
authors.lastname: Yanagihara
authors.forename: T
authors.initials: T
authors.lastname: Yuhi
authors.forename: F
authors.initials: F
authors.lastname: Izumi
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000737:chemistry* / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000494:pharmacology; D002460:Cell Line; D006152:Cyclic GMP / Q000378:metabolism; D006162:Guanylate Cyclase / Q000032:analysis*; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor / Q000032:analysis*
chemical_list: D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; C073616:atrial natriuretic factor receptor C; D006152:Cyclic GMP