Clonality analysis of childhood ALL in remission: no evidence of clonal hematopoiesis.
We studied 98 female patients in remission (2-240 months) from childhood ALL to determine the clonality status of their hematopoiesis. Thirty-one (31.6%) were heterozygous at the PGK locus for the BstX1 endonuclease restriction site, permitting X-linked clonality assays to be performed. Two patients were in relapse at the time of study and were excluded. We used the PGK-PCR clonality assay (PPCA) to analyze DNA from PMN and mononuclear cells of the remaining 29 female patients. All (29/29) patients demonstrated polyclonal hematopoiesis. These data show that remission from childhood ALL involves reestablishment of polyclonally derived hematopoiesis in all patients studied.
Busque
L
L
Hematology-Oncology Division, Brigham and Women's Hospital, Longwood Medical Research Center, Boston, MA 02115.
Ilaria
R
R
Tantravahi
R
R
Weinstein
H
H
Gilliland
D G
DG
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000502:physiology*; D006579:Heterozygote; D006801:Humans; D010735:Phosphoglycerate Kinase / Q000235:genetics; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000201:enzymology / Q000235:genetics; D012074:Remission Induction; D015183:Restriction Mapping
D004273:DNA, Neoplasm; D010735:Phosphoglycerate Kinase