Effect of iron availability on expression of the Bradyrhizobium japonicum hemA gene.
Bradyrhizobium japonicum produces delta-aminolevulinic acid, the universal precursor of tetrapyrroles, in a reaction catalyzed by the product of the hemA gene. Expression of the B. japonicum hemA gene is affected by iron availability. Activity of a hemA-lacZ fusion is increased approximately threefold by iron, and RNA analysis indicates that iron regulation is at the level of mRNA accumulation. To our knowledge, this is the first example of an iron-regulated heme biosynthetic gene in prokaryotes.
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Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755.
Connolly
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Guerinot
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D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000622:Aminolevulinic Acid / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D016384:Consensus Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012230:Rhizobiaceae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D001426:Bacterial Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D000622:Aminolevulinic Acid; D007501:Iron; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase; D001616:beta-Galactosidase