title: Characterization of the prv43 gene of pseudorabies virus and demonstration that it is not required for virus growth in cell culture.
abstract: We have determined the complete DNA sequence of the prv43 gene of a swine herpesvirus, pseudorabies virus. prv43 is 1119 bp in length with a G+C content of 74.5% and is predicted to encode a multiply hydrophobic protein with a molecular mass of 38 kDa. prv43 is colinear with the herpes simplex virus type 1 UL43 locus, and the prv43 and UL43 gene products are predicted to be 47% similar and 23% identical. prv43 is an early gene, producing a 1.2-kb transcript that is easily detected at 2 hr postinfection but not at 6 hr postinfection. We have constructed a prv43 deletion mutant that does not express a prv43 transcript. This mutant did not appear to be defective for viral growth and demonstrated that the gene is nonessential for virus growth in cell culture.
authors.affiliation: Department of Microbiology and Immunology, University of Tennessee at Memphis 38163.
authors.forename: L
authors.initials: L
authors.lastname: Powers
authors.forename: K S
authors.initials: KS
authors.lastname: Wilkinson
authors.forename: P
authors.initials: P
authors.lastname: Ryan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral; D005814:Genes, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000254:growth & development; D017874:Immediate-Early Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D017874:Immediate-Early Proteins; D014764:Viral Proteins; C047583:pseudorabies virus immediate early proteins