title: Effects of new mutations in the spoIIAB gene of Bacillus subtilis on the regulation of sigma F and sigma G activities.
abstract: The spoIIAB gene of Bacillus subtilis encodes an inhibitor of sigma F, a transcription factor that plays a crucial role in the establishment of prespore-specific gene expression during sporulation. The SpoIIAB protein can probably also inhibit a closely related sigma factor sigma G, which determines the later phase of prespore-specific transcription. We have isolated two new missense mutations in the spoIIAB gene. spoIIAB28 behaves like the previously described spoIIAB1 mutation, in that it mainly affects the activity of sigma G. In contrast, the spoIIAB22 mutation seems to be impaired mainly in its ability to inhibit sigma F. All three missense mutations are clustered in the N-terminal coding region of spoIIAB, suggesting that this region of the protein interacts with the sigma factors. The extreme N-terminal part of SpoIIAB may be specifically concerned with the regulation of sigma G activity.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, UK.
authors.forename: D
authors.initials: D
authors.lastname: Foulger
authors.forename: J
authors.initials: J
authors.lastname: Errington
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017354:Point Mutation; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor / Q000037:antagonists & inhibitors*; D013171:Spores, Bacterial / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C064971:FliA protein, Bacteria; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus