An electrophoretic procedure to detect three key enzymes of the ascorbate system.
A method for detection of ascorbic acid oxidase, ascorbic acid peroxidase and dehydroascorbic acid reductase is reported. This method allows the qualitative determination of the presence of these enzymes, also in conditions where the commonly used spectrophotometric assays are unreliable.
De Tullio
M
M
Istituto Botanico, Università di Bari.
Paciolla
C
C
De Gara
L
L
D001204:Ascorbate Oxidase / Q000032:analysis*; D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000378:metabolism*; D003683:Dehydroascorbic Acid / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005638:Fruit / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis; D010088:Oxidoreductases / Q000032:analysis*; D010544:Peroxidases / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D007527:Isoenzymes; D010940:Plant Proteins; D010088:Oxidoreductases; D001204:Ascorbate Oxidase; D010544:Peroxidases; D060387:Ascorbate Peroxidases; C020666:glutathione dehydrogenase (ascorbate); D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid