Disruption of microtubules induces formation of actin fibrils in density-inhibited 3T3 cells.
In density-inhibited 3T3 fibroblast cultures F-actin is localized almost exclusively at the cell-to-cell borders. Depolymerization of microtubules results in a rearrangement of the F-actin pattern. Numerous actin bundles are formed, most of them are anchored to the cell borders. This rearrangement is preceded by a transient rise in F-actin content.
Kajstura
J
J
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
Bereiter-Hahn
J
J
D016475:3T3 Cells; D000199:Actins / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D005347:Fibroblasts / Q000166:cytology; D016650:Fluorescein-5-isothiocyanate; D007700:Kinetics; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure*; D015739:Nocodazole / Q000494:pharmacology; D013997:Time Factors
D000199:Actins; D016650:Fluorescein-5-isothiocyanate; D015739:Nocodazole