[The effect of stimulation of the mononuclear phagocyte system on the binding of high- and low-density lipoproteins by the hepatocytes, Kupffer cells and liver endotheliocytes of rats].
The role of the mononuclear phagocyte system (MPS) in the regulation of low- (LDL) and high-density lipoprotein (HDL) receptor activity in different rat liver cells was investigated. MPS was activated by intravenous administration of bacterial lipopolysaccharide (LPS) from Serratia marcescens. Liver cells were isolated by in vitro perfusion of the liver with a collagenase solution. Separation of Kupffer and endothelial cells was performed by the method of centrifugal elutriation. In control rats, the hepatocytes bound 1.6 times more 125I-HDL and 2.5 times more 125I-LDL per cell than Kupffer cells. Treatment of rats with LPS resulted in a 4.5-fold decrease in the 125I-HDL binding to Kupffer cells. In contrast, the hepatocytes from LPS-treated rat bound 2 times more 125I-HDL than that from untreated rats. The binding of 125I-LDL and 125I-HDL to endothelial cells and 125I-LDL to hepatocytes were not affected by LPS treatment. These results suggest that the MPS (especially Kupffer cells) plays an important role in the regulation of HDL receptor activity in hepatocytes.
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D000818:Animals; D004727:Endothelium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007457:Iodine Radioisotopes; D007728:Kupffer Cells / Q000187:drug effects / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000502:physiology*; D008075:Lipoproteins, HDL / Q000187:drug effects / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010586:Phagocytes / Q000187:drug effects / Q000502:physiology*; D011354:Prodigiozan / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical; D013997:Time Factors
D007457:Iodine Radioisotopes; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D011354:Prodigiozan
Vliianie stimuliatsii sistemy mononuklearnykh fagotsitov na sviazyvanie lipoproteinov vysokoĭ i nizkoĭ plotnosti gepatotsitami, kupferovskimi i éndotelial'nymi kletkami pecheni krys.