Isolation of a 6.2 kb genomic fragment carrying the Adh1 gene of tomato and its expression in transgenic tobacco.
An 11 kb Eco RI genomic fragment containing the alcohol dehydrogenase (Adh1) gene was cloned. Cross-hybridization with three Adh2 cDNA clones suggested that the entire coding region of the Adh1 gene was contained on a 6.2 kb Xba I/Hind III subfragment. Using RFLP linkage analysis, the genomic clone was mapped on chromosome 4 between the markers TG 182 and TG 65 in a position corresponding to the Adh1 locus. To further confirm the Adh1 origin of the genomic clone, tobacco plants were transformed with the 6.2 kb Xba I/Hind III genomic subfragment. Isozyme analysis demonstrated that in transgenic tobacco plants functional tomato specific ADH-1 homodimers were synthesized as well as heterodimers composed of tobacco and tomato subunits.
Wisman
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Department of Plant Breeding, Agricultural University, Wageningen, Netherlands.
Bergervoet
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M
Verkerk
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R
Zabel
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D000426:Alcohol Dehydrogenase / Q000235:genetics*; D003001:Cloning, Molecular; D017343:Genes, Plant; D016678:Genome; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D014026:Nicotiana / Q000235:genetics; D014675:Vegetables / Q000201:enzymology / Q000235:genetics
D000426:Alcohol Dehydrogenase