title: A radiation hybrid map of the BRCA1 region.
abstract: A locus on chromosome 17q, designated "BRCA1," has been identified as a predisposition gene for breast cancer. A panel of chromosome 17-specific radiation-reduced somatic cell hybrid clones has been assembled for high-resolution mapping of chromosome 17. A series of 35 markers, known to span the BRCA1 locus, were tested against this hybrid panel by PCR assays. Statistical analysis of these data yields a BRCA1 radiation hybrid map at a density sufficient to initiate YAC cloning and pulsed-field gel electrophoretic mapping of the candidate region. In addition, many of the markers reveal genetic polymorphisms and may be tested in breast cancer families and in loss-of-heterozygosity studies of sporadic breast cancers to better define the BRCA1 gene candidate region.
authors.affiliation: Department of Pathology, University of Texas Health Science Center, San Antonio 78284.
authors.forename: P
authors.initials: P
authors.lastname: O'Connell
authors.forename: H
authors.initials: H
authors.lastname: Albertsen
authors.forename: N
authors.initials: N
authors.lastname: Matsunami
authors.forename: T
authors.initials: T
authors.lastname: Taylor
authors.forename: J E
authors.initials: JE
authors.lastname: Hundley
authors.forename: T L
authors.initials: TL
authors.lastname: Johnson-Pais
authors.forename: B
authors.initials: B
authors.lastname: Reus
authors.forename: E
authors.initials: E
authors.lastname: Lawrence
authors.forename: L
authors.initials: L
authors.lastname: Ballard
authors.forename: R
authors.initials: R
authors.lastname: White
mesh_terms: D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D006528:Carcinoma, Hepatocellular; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D017931:DNA Primers; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D005819:Genetic Markers