title: Functional oligomerization of purified human papillomavirus types 16 and 6b E7 proteins expressed in Escherichia coli.
abstract: Purified non-fused soluble human papillomavirus type 16 and 6b E7 proteins expressed in Escherichia coli were found to form oligomers. For both proteins, several degrees of oligomerization were demonstrated by gel filtration, dynamic laser light scattering and scanning electron microscopy. Oligomerization was dependent on the concentration of E7 protein. Oligomerized E7 proteins were able to bind the retinoblastoma gene product pRB and stimulated DNA synthesis when introduced into cells.
authors.affiliation: Department of Virology, Kurume University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Chinami
authors.forename: S
authors.initials: S
authors.lastname: Sasaki
authors.forename: N
authors.initials: N
authors.lastname: Hachiya
authors.forename: K
authors.initials: K
authors.lastname: Yuge
authors.forename: T
authors.initials: T
authors.lastname: Ohsugi
authors.forename: H
authors.initials: H
authors.lastname: Maeda
authors.forename: M
authors.initials: M
authors.lastname: Shingu
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry* / Q000378:metabolism; D027383:Papillomaviridae; D050725:Papillomavirus E7 Proteins; D011108:Polymers; D011994:Recombinant Proteins / Q000737:chemistry; D016160:Retinoblastoma Protein / Q000378:metabolism
chemical_list: D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D011108:Polymers; D011994:Recombinant Proteins; D016160:Retinoblastoma Protein; C059731:oncogene protein E7, Human papillomavirus type 16; C062495:oncogene protein E7, Human papillomavirus type 6; D004247:DNA