title: Two tyrosinase nonapeptides recognized on HLA-A2 melanomas by autologous cytolytic T lymphocytes.
abstract: A number of cytolytic T lymphocyte (CTL) clones derived from several melanoma patients have been found to recognize a majority of melanomas from HLA-A2 patients. We have reported previously that two such CTL clones recognize a product of the tyrosinase gene that is presented by HLA-A2. Here we show that one of these CTL clones recognizes a peptide encoded by the first nine amino acids of the putative signal sequence of tyrosinase. The other CTL clone recognizes a different tyrosinase peptide corresponding to amino acids 368-376. Both peptides contain consensus motifs of HLA-A2 binding peptides.
authors.affiliation: I. Medizinische Klinik und Poliklinik, Johannes Gutenberg-Universität, Mainz, FRG.
authors.forename: T
authors.initials: T
authors.lastname: Wölfel
authors.forename: A
authors.initials: A
authors.lastname: Van Pel
authors.forename: V
authors.initials: V
authors.lastname: Brichard
authors.forename: J
authors.initials: J
authors.lastname: Schneider
authors.forename: B
authors.initials: B
authors.lastname: Seliger
authors.forename: K H
authors.initials: KH
authors.lastname: Meyer zum Büschenfelde
authors.forename: T
authors.initials: T
authors.lastname: Boon
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003602:Cytotoxicity, Immunologic; D017931:DNA Primers / Q000737:chemistry; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008545:Melanoma / Q000276:immunology*; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D011994:Recombinant Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D015789:HLA-A2 Antigen; D010455:Peptides; D011994:Recombinant Proteins; D014442:Monophenol Monooxygenase