title: Cloning and expression of the EP3-subtype of human receptors for prostaglandin E2.
abstract: Prostaglandin E2 (PGE2) is a potent mediator in many human tissues that is recognized by three distinct subtypes of receptors (Rs), designated EP1, EP2 and EP3. A cDNA from a human kidney library encodes a 367 amino acid protein, of 85% homology with the mouse EP3R, that is expressed predominantly in kidney tissue. Human EP3Rs of transfectants bound [3H]-PGE2 with a mean Kd of 2.6 nM and native specificity. In contrast to the reliance of EP2Rs on increases in the intracellular concentration of cyclic AMP ([cAMP]i) for cellular signaling, EP3Rs transduce both decreases in [cAMP]i and modest increases in [Ca++]i.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143-0711.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Xia
authors.forename: E J
authors.initials: EJ
authors.lastname: Goetzl
authors.forename: S
authors.initials: S
authors.lastname: An
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008407:Mast Cells / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018078:Receptors, Prostaglandin E / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018078:Receptors, Prostaglandin E; D011994:Recombinant Proteins; D015232:Dinoprostone