title: Clonality, expression and methylation patterns of the Epstein-Barr virus genomes in lethal midline granulomas classified as peripheral angiocentric T cell lymphomas.
abstract: We analysed the terminal repeats of Epstein-Barr virus (EBV) in DNAs isolated from six lethal midline granuloma (LMG) biopsies. A single fused terminal fragment could be detected in each case, indicating that these angiocentric peripheral T cell lymphomas represent clonal proliferations of cells infected with EBV on a single occasion. Using reverse transcriptase-PCR, we detected EBV nuclear antigen (EBNA) 1 and latent membrane protein (LMP) 1, but not EBNA 2 messages in LMG biopsy RNAs. The splicing pattern of the EBNA 1 message was consistent with the usage of a promoter localized in the BamHI F fragment (F promoter). The BamHI W fragment repeats and LMP-coding sequences were highly methylated in all cases. In contrast, the LMP regulatory sequences were found to be hypomethylated or partially methylated, as in LMP-expressing nasopharyngeal carcinomas.
authors.affiliation: Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Minarovits
authors.forename: L F
authors.initials: LF
authors.lastname: Hu
authors.forename: S
authors.initials: S
authors.lastname: Imai
authors.forename: Y
authors.initials: Y
authors.lastname: Harabuchi
authors.forename: A
authors.initials: A
authors.lastname: Kataura
authors.forename: S
authors.initials: S
authors.lastname: Minarovits-Kormuta
authors.forename: T
authors.initials: T
authors.lastname: Osato
authors.forename: G
authors.initials: G
authors.lastname: Klein
mesh_terms: D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D002999:Clone Cells; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D019309:Epstein-Barr Virus Nuclear Antigens; D005260:Female; D006103:Granuloma, Lethal Midline / Q000382:microbiology* / Q000473:pathology; D006566:Herpesviridae Infections / Q000382:microbiology; D004854:Herpesvirus 4, Human / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D016411:Lymphoma, T-Cell, Peripheral / Q000382:microbiology* / Q000473:pathology; D008297:Male; D008745:Methylation; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000032:analysis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D014158:Transcription, Genetic; D014412:Tumor Virus Infections / Q000382:microbiology; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D009856:Oncogene Proteins, Viral; D014763:Viral Matrix Proteins