Connectin, giant elastic protein, in giant sarcomeres of crayfish claw muscle.
In the giant sarcomeres (sarcomere length, 10 microns at rest) of crayfish claw muscle, 3000 kDa connectin-like protein but not projectin (mini-titin) appears to be responsible for passive tension generation. Proteolysis of crayfish connectin in skinned fibres was parallel with disappearance of resting tension. Immunofluorescence observations using the antiserum to crayfish connectin showed that crayfish connectin linked the A band to the Z line in a giant sarcomere. It appears that crayfish connectin exerts a centering force on the A band in a sarcomere. Very thin filaments in the I band were visualized after the actin filaments had been removed by the treatment with plasma gelsolin. Crayfish connectin was partially purified and its rotary shadowed image was a very long filament. Projectin was localized on the A band of crayfish giant sarcomeres and remained unmoved during stretch or contraction. However, on dissolution of myosin filaments, projectin moved to the Z line together with crayfish connectin. It seems that projectin binds to connectin on the myosin filament. In regular size of sarcomeres (sarcomere lengths, 3-4 microns at rest) of crayfish stretcher muscle, projectin linked the A band to the Z line, as in insect flight muscle.
Manabe
T
T
Department of Biology, Faculty of Science, Chiba University, Japan.
Kawamura
Y
Y
Higuchi
H
H
Kimura
S
S
Maruyama
K
K
D000818:Animals; D003400:Astacoidea / Q000737:chemistry*; D064211:Connectin; D004548:Elasticity; D005455:Fluorescent Antibody Technique; D018260:Gelsolin; D015151:Immunoblotting; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D009119:Muscle Contraction / Q000502:physiology; D009124:Muscle Proteins / Q000032:analysis* / Q000502:physiology; D009126:Muscle Relaxation / Q000502:physiology; D009132:Muscles / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure*; D011494:Protein Kinases; D012518:Sarcomeres / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure
D064211:Connectin; D018260:Gelsolin; D009124:Muscle Proteins; C078033:projectin; D011494:Protein Kinases