Pneumonia in pigs inoculated with pseudorabies virus by means of a bronchoscope.
Pigs inoculated endobronchially (EB) with 2 ml of virus suspension containing 10(4) TCID50 per ml of the YS-81 strain of pseudorabies virus (PRV), by means of a bronchoscope, all developed viral pneumonia. No pneumonic lesions were observed in intranasally inoculated pigs. Macroscopical and microscopical lesions were localized to the middle to caudal parts of the right caudal lobe and were closely associated with the site at which the inoculum was deposited. PRV became attached to all types of cells and caused destruction of epithelial cells, and viral antigen persisted in the alveolar macrophages. After PRV infection, the total cell number in broncho-alveolar lavage (BAL) fluid was slightly increased and a high titre of PRV was found in the cells of BAL fluid in EB infected pigs. The findings suggest that PRV infection leads to dysfunction of alveolar macrophages before cell death is produced by virus replication.
Narita
M
M
National Institute of Animal Health, Ibaraki, Japan.
Kawashima
K
K
Matsuura
S
S
Uchimura
A
A
Miura
Y
Y
D000818:Animals; D011558:Herpesvirus 1, Suid / Q000302:isolation & purification*; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D008854:Microscopy, Electron / Q000662:veterinary; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D011024:Pneumonia, Viral / Q000150:complications / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D011557:Pseudorabies / Q000150:complications / Q000382:microbiology / Q000473:pathology*; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000473:pathology*