A rapid and simple method to isolate and characterize highly polymorphic markers from the centromeric regions of the human chromosomes.
Using oligonucleotide primers complementary to the 3' ends of either the Alu or the L1Hs consensus sequences in conjunction with a primer complementary to alpha satellite subsets specific to different human chromosomes, it was possible to detect and characterize polymorphisms originating from the microsatellites which are often present downstream these repetitive elements. The methodology does not require cloning, sequencing or synthesis of specific primers. Centromeric location was confirmed by linkage analysis, in situ hybridization and sequencing. The method is proposed for the generation of polymorphic markers from all centromeric regions.
Laurent
A M
AM
UPR 9008 CNRS, Montpellier, France.
Marçais
B
B
Muleris
M
M
Roizès
G
G
D001483:Base Sequence; D002503:Centromere; D002877:Chromosomes, Human; D017931:DNA Primers; D005819:Genetic Markers; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D017931:DNA Primers; D005819:Genetic Markers