The effect of a heterocyclic amine, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), on tyrosine hydroxylase mRNA in PC12h cells.
The effects of a naturally occurring heterocyclic amine, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indol (Trp-P-1), on tyrosine hydroxylase (TH) mRNA levels were examined in PC12h cells using Northern blot analysis. Dose-dependency of Trp-P-1 for 24 h incubation gave biphasic results; 0.1 microM Trp-P-1 enhanced and 10 microM Trp-P-1 reduced TH mRNA levels. These effects were faded out in 48 h. One hundred microM Trp-P-1 caused the almost complete abolishment of TH mRNA expression as well as cell death. These results suggest the possibility that lower dose of Trp-P-1 might function as a trophic factor in contrast to neurotoxic effect in higher doses.
Ota
A
A
Joint Research Division for Therapies against Intractable Diseases, Fujita Health University, Aichi, Japan.
Maruyama
W
W
Takahashi
T
T
Naoi
M
M
Nagatsu
T
T
D000818:Animals; D015152:Blotting, Northern; D002243:Carbolines / Q000494:pharmacology* / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects; D002999:Clone Cells; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology* / Q000633:toxicity; D016716:PC12 Cells; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis*
D002243:Carbolines; D009153:Mutagens; D012333:RNA, Messenger; D003609:Dactinomycin; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; D003513:Cycloheximide; D014446:Tyrosine 3-Monooxygenase; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole