title: Evaluation of the ADx REA assay for determination of ethanol in serum and urine.
abstract: The Abbott ADx REA assay (ADx) for the determination of ethanol in human serum and urine was evaluated. The within-run precision of a urine sample containing 3.00 g/L ethanol resulted in a coefficient of variance (CV) of 2.1% (n = 20). The within-run precision of a serum sample containing 1.47 g/L ethanol resulted in a CV of 2.3% (n = 20). The between-run precision of the TDx REA ethanol assay controls yielded 0.50 +/- 0.02 g/L ethanol (target value, 0.50 g/L) with a CV of 3.0% (n = 6); 1.03 +/- 0.03 g/L ethanol (target value, 1.00 g/L) with a CV of 2.7% (n = 6); and 2.56 +/- 0.07 g/L ethanol (target value, 2.50 g/L) with a CV of 2.8% (n = 6). Results of an intermethod comparison study of clinical serum and urine ethanol determinations by gas chromatography (x) and ADx (y) yielded by regression analysis y = 0.993x - 0.04 g/L (r = 0.985; n = 92) for serums and y = 0.974x - 0.02 g/L, (r = 0.991; n = 75) for urines. No significant interference was seen with low molecular weight alcohols or ketones.
authors.affiliation: Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond.
authors.forename: S A
authors.initials: SA
authors.lastname: Jortani
authors.forename: A
authors.initials: A
authors.lastname: Poklis
mesh_terms: D002624:Chemistry, Clinical / Q000295:instrumentation*; D002849:Chromatography, Gas; D000431:Ethanol / Q000097:blood* / Q000652:urine*; D006801:Humans; D000091402:Prohibitins; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: C000717331:PHB2 protein, human; D000091402:Prohibitins; D000431:Ethanol