title: Heat shock does not induce tolerance to hyperoxia.
abstract: Thermal stress is associated with the induction of a specific set of proteins called heat shock proteins and with the induction of thermal tolerance. Heat stress has been shown to be capable of inducing at least partial tolerance to other stresses, including some oxidant stresses. Furthermore, these oxidant stresses are reported to be inducers of heat shock proteins. We hypothesized that hyperoxic stress would induce heat shock proteins and that factors induced by thermal stress, including heat shock proteins, would offer at least partial protection from hyperoxic exposure. We were particularly interested in a level of protection that would be relevant to clinical situations. Lung fibroblasts and live animals were exposed to thermal stress and/or hyperoxic stress and examined for induction of HSP70 (the most conserved of the heat shock proteins) and for induced tolerance as determined by the ability of cells to metabolize 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and by comparison of lung wet to dry weight ratios in live animals. Each stress induced tolerance to itself, but there was no evidence of heat stress inducing tolerance to hyperoxic stress. Furthermore, there was only minimal induction of HSP70 mRNA by hyperoxic exposure. We conclude that some overlap of mechanisms of induced tolerance by hyperoxic and thermal stress exists, but that differences far outweigh similarities.
authors.affiliation: Yale University, Department of Pediatrics, New Haven, Connecticut 06510.
authors.forename: C
authors.initials: C
authors.lastname: Strand
authors.forename: J B
authors.initials: JB
authors.lastname: Warshaw
authors.forename: K
authors.initials: K
authors.lastname: Snow
authors.forename: H C
authors.initials: HC
authors.lastname: Jacobs
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006358:Hot Temperature / Q000009:adverse effects*; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology / Q000378:metabolism* / Q000503:physiopathology; D008928:Mitochondria / Q000378:metabolism; D010100:Oxygen / Q000009:adverse effects*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000503:physiopathology*; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D013778:Tetrazolium Salts; D010100:Oxygen