Secretion of transforming growth factor-beta by the immature rat prostate.
Immature rat ventral prostate was used to identify a potent cell growth inhibitory factor in normal prostate. Both conditioned media and peptide extracts derived from ventral prostates inhibit the cellular growth and DNA synthesis in metastatic androgen-independent human prostate carcinoma cell line (PC3). The prostatic inhibitory factor was partially purified using an hydrophobic HPLC column (C18). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis (SDS-PAGE) of partially purified material revealed a major 25-kDa band. Immunoblot analysis showed similarity of the inhibitory factor to the transforming growth factor-beta (TGF-beta). Furthermore, the proliferation inhibiting activity of the prostatic factor was completely abolished by anti-TGF-beta antibodies. These data indicate that normal prostate produces a TGF-beta-like protein under non-physiological conditions.
Atfi
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Centre Régional de Recherche en Endocrinologie, Faculté de Médecine-Pharmacie, Rennes, France.
Samperez
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Jouan
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D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008297:Male; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011467:Prostate / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000473:pathology*; D051381:Rats; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D010455:Peptides; D016212:Transforming Growth Factor beta