Structure of pentameric human serum amyloid P component.
The three-dimensional structure of pentameric human serum amyloid P component at high resolution, the first reported for a pentraxin, reveals that the tertiary fold is remarkably similar to that of the legume lectins. Carboxylate and phosphate compounds bind directly to two calcium ions; interactions with a carboxyethylidene ring are mediated by Asn 59 and Gln 148 ligands of the calcium ions. These X-ray results indicate the probable modes of binding of the biologically important ligands, DNA and amyloid fibrils.
Emsley
J
J
Laboratory of Molecular Biology, Birkbeck College, London, UK.
White
H E
HE
O'Hara
B P
BP
Oliva
G
G
Srinivasan
N
N
Tickle
I J
IJ
Blundell
T L
TL
Pepys
M B
MB
Wood
S P
SP
D000595:Amino Acid Sequence; D000686:Amyloidosis / Q000378:metabolism; D001665:Binding Sites; D002097:C-Reactive Protein / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003196:Computer Graphics; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008756:Methylgalactosides / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010767:Phosphorylcholine / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D000683:Serum Amyloid P-Component / Q000737:chemistry* / Q000378:metabolism
D008024:Ligands; D008756:Methylgalactosides; D010714:Phosphatidylethanolamines; D000683:Serum Amyloid P-Component; D010767:Phosphorylcholine; D002097:C-Reactive Protein; D004247:DNA; C042451:methyl 4,6-O-(1-carboxyethylidene)galactopyranoside; D010450:Endopeptidases; D002118:Calcium