Solution structure of pyoverdin GM-II.
The three-dimensional structure in solution of ferri-pyoverdin GM-II isolated from the culture medium of Pseudomonas fluorescens was determined by application of NMR methods to the Ga3+ analogue. Distance geometry calculations were performed with FILMAN using interproton distances and coupling constants as constraints. Further conformational analysis was carried out by energy minimization with MM2 and AMBER. Back-calculation of the NOESY spectra shows that the resulting structures are in agreement with the experimental data.
Mohn
G
G
Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, Strasbourg, France.
Koehl
P
P
Budzikiewicz
H
H
Lefèvre
J F
JF
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D003470:Culture Media; D005708:Gallium; D007502:Iron Chelating Agents / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification; D010860:Pigments, Biological / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D011551:Pseudomonas fluorescens / Q000378:metabolism*
D003470:Culture Media; D007502:Iron Chelating Agents; D009842:Oligopeptides; D010860:Pigments, Biological; C086297:pyoverdin GM-II; C042453:pyoverdin; D005708:Gallium