title: Crystallization and preliminary X-ray analysis of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus.
abstract: X-ray quality single crystals of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus were obtained by the hanging drop method. The intradiol dioxygenase crystallizes in the cubic space group I23 with unit cell dimensions a = b = c = 145.5 A. The dodecahedral crystals diffract to beyond 2.5 A resolution. The asymmetric unit contains one twelfth of the enzyme (alpha beta Fe+3)12 complex.
authors.affiliation: Department of Biochemistry, University of Minnesota, Minneapolis 55455.
authors.forename: M W
authors.initials: MW
authors.lastname: Vetting
authors.forename: C A
authors.initials: CA
authors.lastname: Earhart
authors.forename: D H
authors.initials: DH
authors.lastname: Ohlendorf
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D011520:Protocatechuate-3,4-Dioxygenase / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D011520:Protocatechuate-3,4-Dioxygenase