Evidence of a role for intestinal CD8+ lymphocytes and macrophages in transport of Eimeria acervulina sporozoites.
The objective of this study was to identify host immune cells interacting with Eimeria sporozoites in the intestine. One-week-old SC strain chickens were infected with 2 x 10(7) sporulated Eimeria acervulina oocysts. Twenty-four hours postinfection, duodenal sections were removed, imbedded in Tissue-Tek compound, and frozen. After sectioning, tissues were stained with monoclonal antibodies to identify CD4+, CD8+, Ig+, and Bula+ lymphocytes, macrophages, and sporozoites; 28% of the sporozoites were in CD8+ cells, 28% in macrophages, and 12% in CD4+ cells. Sporozoites were not seen in Ig+ or Bula+ cells. These findings suggest that sporozoite transport most likely occurs in T lymphocytes and macrophages.
Trout
J M
JM
Protozoan Diseases Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Lillehoi
H S
HS
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000469:parasitology; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D004386:Duodenum / Q000276:immunology* / Q000469:parasitology; D004539:Eimeria / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D008264:Macrophages / Q000469:parasitology; D010587:Phagocytosis; D011201:Poultry Diseases / Q000276:immunology* / Q000469:parasitology; D050378:T-Lymphocytes, Regulatory / Q000469:parasitology