Cloning of a complete protein-coding sequence of human platelet-type phosphofructokinase isozyme from pancreatic islet.
We have cloned a full length protein-coding sequence of human platelet-type phosphofructokinase (PFK) from pancreatic islet cDNA library. The platelet-type PFK was composed of 784 amino acids and had a deduced molecular weight of 85,590. Homologies in the primary structure with muscle- and liver-type PFK were 71 and 67%. Clear similarities of the amino and carboxyl halves with a prokaryotic PFK indicated an evolutionary event that duplicated genes of a prototype PFK fused into larger genes of eukaryotic PFKs. Amino acid residues constituting the binding sites for various allosteric modulators were well conserved, while a couple of different residues at the inhibitory ATP sites among three isozymes may partly explain their varied degree of sensitivities to ATP. Considerable amount of platelet-type PFK expression was demonstrated in brain, heart, kidney, colon and testis.
Eto
K
K
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Sakura
H
H
Yasuda
K
K
Hayakawa
T
T
Kawasaki
E
E
Moriuchi
R
R
Nagataki
S
S
Yazaki
Y
Y
Kadowaki
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001792:Blood Platelets / Q000201:enzymology*; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D003001:Cloning, Molecular; D003106:Colon / Q000201:enzymology; D017931:DNA Primers; D005260:Female; D006801:Humans; D007515:Islets of Langerhans / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D007668:Kidney / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D010732:Phosphofructokinase-1 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D010920:Placenta / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology
D017931:DNA Primers; D007527:Isoenzymes; D012333:RNA, Messenger; D010732:Phosphofructokinase-1