[The effect of aflatoxin B1 on the human lymphocyte blast transformation reaction].
The effect of various aflatoxin B1 concentrations on human peripheral blood lymphocyte blast-transformation reaction was studied. There was aflatoxin B1-induced dose-dependent inhibition of DNA synthesis in the lymphocytes stimulated by phytohemagglutinin. The aflatoxin B1 concentrations causing 50% decreases in DNA synthesis were defined by the regression method. (ID50). Judging from the ID50 values, the treatment of cultured lymphocytes with cytochrome P-448 inductor and lymphocytic exposure in the presence of toxin and hepatic microsomes of mice showed a 6-7-fold increase in the inhibitory action of aflatoxin B1. This indicates that the metabolism of aflatoxin B1 brought about by the cytochrome P-450-dependent monooxygenase system plays an important role in the action of aflatoxin B1 on human lymphocytes.
Astashkin
E I
EI
Nikolaeva
I S
IS
Riabichenko
V V
VV
Prikhod'ko
A Z
AZ
D016604:Aflatoxin B1 / Q000494:pharmacology*; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D003864:Depression, Chemical; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology
D010835:Phytohemagglutinins; D004247:DNA; D016604:Aflatoxin B1
Vliianie aflatoksina B1 na reaktsiiu blasttransformatsii limfotsitov cheloveka.