Expression of intercellular adhesion molecule-1 on rat microglial cells.
Microglial cells isolated from newborn rat brain displayed a baseline immunoreactivity for intercellular adhesion molecule-1 (ICAM-1) after 10-20 days in culture. ICAM-1 immunoreactivity was enhanced in a time-dependent fashion by exposing the microglial cells to recombinant rat gamma-interferon (gamma-IFN; 100 U/ml) or tumor necrosis factor-alpha (TNF-alpha; 1000 U/ml). We conclude that rat brain macrophages/microglial cells can be induced by gamma-IFN and TNF-alpha to express ICAM-1. This may be relevant in the interaction of microglial cells with other brain cells during inflammation of the central nervous system.
Zielasek
J
J
Department of Neurology, Julius-Maximilians Universität Würzburg, FRG.
Archelos
J J
JJ
Toyka
K V
KV
Hartung
H P
HP
D000818:Animals; D000831:Animals, Newborn; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002478:Cells, Cultured; D005434:Flow Cytometry; D005453:Fluorescence; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000494:pharmacology; D009457:Neuroglia / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma