Effect of insulin induced hypoglycemia on acetylcholinesterase and Na+, K(+)-ATPase activity of rat heart, liver and kidney.
The effect of insulin induced hypoglycemia was studied on the activities of Acetylcholinesterase (AChE) which hydrolizes acetylcholine (ACh) and Na+, K(+)-ATPase, the enzymatic version of Na+,K(+)-pump from rat heart, liver and kidney. AChE activity was decreased significantly from two subcellular fractions of all the three tissues after 1,2 and 3 hours of insulin administration (5 units/100 gm body weight) Na+, K(+)-ATPase activity was decreased in liver and kidney, whereas, a significant increase in heart ATPase activity was observed. Blood glucose level was significantly lower than control values after 1, 2 and 3 hours of insulin injection, and the protein content from these tissues did not show any significant change under this physiological stress.
Arora
S K
SK
Department of Zoology, Guru Nanak Dev University, Amritsar, India.
Kaur
G
G
D000110:Acetylcholinesterase / Q000032:analysis*; D000208:Acute Disease; D000818:Animals; D001341:Autonomic Nervous System / Q000503:physiopathology; D001786:Blood Glucose / Q000032:analysis; D007003:Hypoglycemia / Q000139:chemically induced / Q000201:enzymology* / Q000503:physiopathology; D007328:Insulin / Q000633:toxicity*; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D011506:Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*
D001786:Blood Glucose; D007328:Insulin; D011506:Proteins; D000110:Acetylcholinesterase; D000254:Sodium-Potassium-Exchanging ATPase