Liquid chromatography and capillary electrophoresis analysis of polyanionic quinobene.
Quinobene is the tetrasodium salt of an organic tetrasulphonic acid. Its unusual solubility characteristics makes the development of LC analysis difficult. However, a specific, precise and accurate LC assay was eventually achieved for quinobene. The assay required gradient elution and was not efficient for quinobene with respect to plate number. As an alternative, a capillary electrophoresis (CE) assay was also developed for quinobene. The CE assay was comparable to the LC assay in precision and accuracy. It was unaffected by the unusual solubility characteristics of quinobene and was more specific, efficient and rugged than the LC assay.
Cheung
A P
AP
Life Sciences Division, SRI International, Menlo Park, CA 94025.
Nguyenle
T
T
Hettiarachchi
K
K
D000998:Antiviral Agents / Q000032:analysis*; D001391:Azo Compounds / Q000032:analysis*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004586:Electrophoresis; D006801:Humans; D011863:Radioimmunoassay; D012015:Reference Standards; D013050:Spectrometry, Fluorescence; D013267:Stilbenes / Q000032:analysis*
D000998:Antiviral Agents; D001391:Azo Compounds; D013267:Stilbenes; C076933:quinobene