title: Decreased postnatal testosterone surge in male rats exposed to ethanol during the last week of gestation.
abstract: Prenatal alcohol exposure in the rat is known to interfere with the neurobehavioral sexual differentiation of the male brain. Because normal sexual differentiation of the male brain requires adequate levels of perinatal testosterone, we examined the effect of prenatal ethanol exposure on (1) the postnatal surge of testosterone and (2) the in vitro secretion of testosterone in response to luteinizing hormone (LH) stimulation of testes from fetal alcohol exposed (FAE) animals and controls. Sprague-Dawley dams were administered a fortified liquid diet containing 35% ethanol-derived calories, a pair-fed (PF) isocaloric liquid diet, or given ad libitum access to dry lab chow (CF). Dams were administered the liquid diets from days 7 or 14 through parturition. The postnatal surge of testosterone in FAE males was studied only in animals exposed to ethanol from day 14 through parturition. In the first experiment, FAE and PF males and females were delivered by cesarean section on day 22 of gestation (E22) and trunk blood collected at 0, 60, 120, and 240 min after parturition. Experiment 2 measured plasma testosterone in male pups that were killed at 0, 60, 120, 240, 360, and 480 min after delivery. Results showed that the postnatal testosterone surge of FAE males in both experiments was significantly attenuated compared with PF controls. No effect of prenatal ethanol was observed in female offspring. Female testosterone levels were several fold lower than male littermates, and no evidence of a postnatal testosterone surge was observed. Production of testosterone from testes was studied using an automated perifusion system.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Psychology, San Diego State University, California.
authors.forename: R F
authors.initials: RF
authors.lastname: McGivern
authors.forename: R J
authors.initials: RJ
authors.lastname: Handa
authors.forename: E
authors.initials: E
authors.lastname: Redei
mesh_terms: D000818:Animals; D000431:Ethanol / Q000633:toxicity; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000473:pathology / Q000503:physiopathology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005865:Gestational Age; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012733:Sex Differentiation / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000187:drug effects / Q000473:pathology; D013739:Testosterone / Q000097:blood*
chemical_list: D000431:Ethanol; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone