Different ATP effects on natriuretic peptide receptor subtypes in LLC-PK1 and NIH-3T3 cells.
We have observed different ATP interactions in two guanylate cyclase (GC)-coupled natriuretic peptide (NP) receptor subtypes, designated NPR-A and NPR-B. The NPR-A is selectively expressed by LLC-PK1 epithelial cells and the NPR-B by NIH-3T3 fibroblast cells. In LLC-PK1 membranes, ATP-Mg2+ potentiated ANP-stimulated GC activity (ANP-s-GC). In contrast, in NIH-3T3 membranes, ATP-Mg2+ inhibited ANP-s-GC but enhanced CNP-stimulated GC activity (CNP-s GC). ATP in the presence of Mn2+ inhibited LLC-PK1 and NIH-3T3 membrane ANP-s-GC and CNP-s-GC. These are the first data suggesting that the ATP-Mg2+ produces different effects between membrane NPR-A and -B subtypes. We have also demonstrated that GC of NPR-B is sensitive to methylene blue.
Shigematsu
Y
Y
Department of Research Biochemistry, Ochsner Clinic, New Orleans, Lousiana.
Vaughn
J
J
Touchard
C L
CL
Frohlich
E D
ED
Alam
J
J
Cole
F E
FE
D016475:3T3 Cells; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D006162:Guanylate Cyclase / Q000378:metabolism / Q000494:pharmacology; D007668:Kidney; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D017461:Receptors, Atrial Natriuretic Factor / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D013552:Swine
D008345:Manganese; D000255:Adenosine Triphosphate; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; D008274:Magnesium