Haplotypes of the human immunoglobulin lambda IGLV7S1 and IGLV1S1 genes defined by restriction-site polymorphism and insertion/deletion of a 6-kb DNA fragment.
Haplotypes were defined in the human immunoglobulin lambda locus by using three probes--V lambda VII, V lambda A, and V lambda I--hybridized to BamHI, KpnI, EcoRI, and HindIII digests. Four KpnI alleles were described. Two of them, 13 kb and 16 kb, detected with both the V lambda VII and V lambda A probes, were correlated with 4.6-kb and 10.5-kb KpnI fragments, respectively, which hybridize to the V lambda I probe. The two others (17 kb and 24 kb) were detected with the three probes V lambda VII, V lambda A, and V lambda I. Moreover, we show that two of those haplotypes may reflect an insertion of 6 kb between V lambda A and V lambda 1S1. Familial studies were performed to demonstrate the Mendelian inheritance. Our results demonstrate the absence of association between the C lambda alleles and V lambda haplotypes.
Chuchana
P
P
Laboratoire d'Immunogénétique Moléculaire, LIGM, URA CNRS 1191, Université Montpellier II, France.
Frippiat
J P
JP
Blancher
A
A
Lefranc
G
G
Lefranc
M P
MP
D000916:Antibody Diversity; D003001:Cloning, Molecular; D015342:DNA Probes; D005787:Gene Frequency; D005803:Genes, Immunoglobulin; D015894:Genome, Human; D006239:Haplotypes; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D008297:Male; D016254:Mutagenesis, Insertional; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D011544:Pseudogenes; D017384:Sequence Deletion
D015342:DNA Probes; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains