title: A microplate system for ABO and Rh(D) blood grouping.
abstract: A microplate system for performing ABO and Rh(D) blood group determinations with a Kemble Kemtek 1000SP liquid handling processor, an Anthos 2001 microplate reader, an IBM Personal System 2 microcomputer, and Sanguin Forma software is described. The performance of this Kemble/Anthos/IBM/Sanguin microplate system for ABO and D grouping was evaluated by testing 10,042 routine blood donor samples in parallel with the forward-grouping channels of a Technicon AutoAnalyzer blood grouping system. Manual techniques were used to perform ABO reverse groupings and to resolve all ABO forward- and reverse-grouping discrepancies. D-negative test results were investigated and confirmed manually by the indirect antiglobulin test. Of the 10,042 samples tested, 97.3 percent were grouped correctly. There were 266 samples whose results were flagged as no type determined, of which 124 were ABO tests and 142 were Rh tests. In addition, 30 weak D samples missed by both automated systems were detected by manual indirect antiglobulin test. The system is flexible and easy to maintain and operate.
authors.affiliation: National Reference Laboratory, Canadian Red Cross Society, Ottawa, Ontario, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Chung
authors.forename: P
authors.initials: P
authors.lastname: Birch
authors.forename: K
authors.initials: K
authors.lastname: Ilagan
mesh_terms: D000017:ABO Blood-Group System; D001331:Automation; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D003298:Coombs Test; D006801:Humans; D008838:Microcomputers; D010641:Phenotype; D012204:Rh-Hr Blood-Group System / Q000276:immunology
chemical_list: D000017:ABO Blood-Group System; D012204:Rh-Hr Blood-Group System