Specific increase of L-dopa levels in plasma upon infusion of tyrosinase containing liposomes.
1. The present paper reports the effects of liposome-entrapped tyrosinase (EC 1.14.18.1. L-Tyrosine, L-3,4-dihydroxyphenylalanine: oxygen oxidoreductase) infusion on the catecholamine contents of rat plasma. The actions of liposomes and free tyrosinase have also been investigated. 2. From the experiments, evidence has been obtained that liposome-entrapped tyrosinase is able to affect specifically L-3,4-dihydroxyphenylalanine (L-DOPA) levels which increase dramatically. 3. The possible use of liposome-entrapped tyrosinase to raise L-DOPA levels in catecholamine related disorders is discussed. 4. Liposomes without tyrosinase provoke no significant changes of catecholamine or L-DOPA levels while free tyrosinase does induce a change but in a less constant fashion than the liposome-entrapped enzyme.
Miranda
M
M
Department of Cell Biology and Physiology, University of L'Aquila, Italy.
Amicarelli
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F
Volpe
A R
AR
Poma
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A
Masciocco
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Carmignani
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D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002395:Catecholamines / Q000097:blood; D004337:Drug Carriers; D006339:Heart Rate / Q000187:drug effects; D007262:Infusions, Intravenous; D007980:Levodopa / Q000097:blood*; D008081:Liposomes; D014442:Monophenol Monooxygenase / Q000008:administration & dosage / Q000494:pharmacology*; D009200:Myocardial Contraction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D002395:Catecholamines; D004337:Drug Carriers; D008081:Liposomes; D007980:Levodopa; D014442:Monophenol Monooxygenase