Micronucleus induction in human B and T lymphocytes separated by an immunomagnetic technique.
Micronuclei were induced in human lymphocytes by mitomycin C or gamma radiation. After the incubation the cells were separated in B- and T-cells by an immunomagnetic method, i.e., Dynabeads. The cultures were stimulated by PHA. The effect of different incubation times (60-110 h) as well as different doses of mitomycin C (0-100 nmol/l) and gamma radiation (0-2 Gy) at an incubation time of 72 h were studied. There were significantly higher frequencies of micronuclei in B- than in T-cells, when micronuclei were induced by mitomycin C. This was not found with gamma radiation, probably because of a cytotoxic effect of this agent on the B-cells.
Högstedt
B
B
Department of Occupational Medicine, Central Hospital, Halmstad, Sweden.
Karlsson
A
A
Bratt
I
I
Holmén
A
A
D001402:B-Lymphocytes / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D018189:Immunomagnetic Separation; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D016685:Mitomycin / Q000494:pharmacology; D008940:Mitotic Index / Q000187:drug effects / Q000528:radiation effects; D013601:T-Lymphocytes / Q000187:drug effects* / Q000528:radiation effects
D016685:Mitomycin