Trichlorobromomethane-induced changes of purine nucleotides in hepatocytes.
The changes in nucleotide content during CBrCl3 treatment were investigated. In the first 5-10 minutes a significant ATP decrease was detected. The GTP loss leading to 57% of the initial level after 10 min surpasses the ATP loss which leads to 70% of the initial value after 10 min. The increase in uric acid is not only the result of CBrCl3 induced reaction, because of no significant changes in adenine and hypoxanthine values. The uric acid pool reflected different influx and efflux processes. These changes were compared with nucleotide degradation and accumulation of nucleotide degradation products during anoxia.
Müller
K
K
Clinics of Physical Therapy and Rehabilitation, Medical Faculty (Charité), Humboldt University Berlin, Germany.
Grune
T
T
Klee
S
S
Jochmann
C
C
Ungemach
F
F
Siems
W
W
D000255:Adenosine Triphosphate / Q000378:metabolism; D000393:Air Pollutants / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D001975:Bromotrichloromethane / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006160:Guanosine Triphosphate / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D011685:Purine Nucleotides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014527:Uric Acid / Q000378:metabolism
D000393:Air Pollutants; D011685:Purine Nucleotides; D014527:Uric Acid; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D001975:Bromotrichloromethane