Involvement of HIV nef protein in abnormal hematopoiesis in AIDS: in vitro study on bone marrow progenitor cells.
In previous studies (1-4), we described disrupted hematopoiesis in long-term human bone marrow cultures infected with HIV. Conditioned medium (CM) from HIV-1 non-productively infected liquid cultures inhibits the proliferation of granulo-monocytic progenitor cells (CFU-GM) in clonogenic assays. A growth-inhibitory soluble factor was therefore suspected and we report here that this factor has been identified as the nef gene product. These nef-containing supernatants are specifically neutralized by anti-nef antibodies and recombinant nef has the same growth inhibitory properties. We used a nef-deficient virus to confirm that nef protein is responsible for the in vitro growth inhibition of CFU-GM.
Calenda
V
V
INSERM Unit 322, Research Unit on Retroviruses and Associated Diseases, Marseille, France.
Graber
P
P
Delamarter
J F
JF
Chermann
J C
JC
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood*; D001853:Bone Marrow / Q000382:microbiology*; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D015693:Gene Products, nef / Q000502:physiology*; D015754:Genes, nef; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000382:microbiology*; D006801:Humans; D066298:In Vitro Techniques; D011994:Recombinant Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; D011994:Recombinant Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus