33-kDa peptides prepared from chicken gizzard smooth muscle bundle both actin and desmin filaments in vitro.
A group of 33-kDa peptides was prepared from chicken gizzard. Gizzard smooth muscle was extracted with Hasselbach-Schneider's high ionic strength solution. The extract was fractionated by ammonium sulfate precipitation. The soluble fraction obtained at 60% ammonium sulfate saturation was dialyzed against 0.1 M KCl. The precipitate formed during the dialysis mainly contained 33-kDa peptides. On SDS-PAGE, two major bands and one minor band were stained reddish purple by Coomassie Brilliant Blue R-250. These peptides, which were insoluble in physiological salt solutions, bundled not only actin filaments but also desmin filaments. Actin was markedly coprecipitated with the 33-kDa peptides on the usual centrifugation at 22,000 x g.
Nakagawa
H
H
Department of Biology, Faculty of Science, Chiba University.
Ishihara
M
M
Ohashi
K
K
D000199:Actins / Q000378:metabolism*; D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D001665:Binding Sites; D005591:Chemical Fractionation; D002645:Chickens; D003893:Desmin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005895:Gizzard, Avian; D007382:Intermediate Filaments / Q000378:metabolism*; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000737:chemistry*; D010455:Peptides / Q000378:metabolism*; D012394:Rosaniline Dyes / Q000737:chemistry
D000199:Actins; D003893:Desmin; D010455:Peptides; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue; D000645:Ammonium Sulfate