Relationship between chimpanzee Rh-like genes and the R-C-E-F blood group system.
The antigenic closeness between the chimpanzee alloantigen Rc of the R-C-E-F system, and the human alloantigen Rho(D) suggests a phyloge-connection between their genes. To confirm at the molecular level the common origin of these genes, genomic DNA from 16 unrelated chimpanzees of various R-C-E-F phenotypes were digested by three restriction enzymes and analyzed by Southern blot using a human Rh cDNA probe and three exon-specific probes. Restrictions profiles displayed reach polymorphism. Correlations between some bands and certain R-C-E-F phenotypes demonstrate that the human Rh cDNA probe defines in chimpanzee genomic DNA some genes of the R-C-E-F system.
Salvignol
I
I
Centre Régional de Transfusion Sanguine, CHU Purpan, Toulouse, France.
Blancher
A
A
Calvas
P
P
Socha
W W
WW
Colin
Y
Y
Cartron
J P
JP
Ruffié
J
J
D000818:Animals; D005075:Biological Evolution; D001789:Blood Group Antigens / Q000235:genetics*; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D006801:Humans; D002679:Pan troglodytes / Q000097:blood* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D013045:Species Specificity
D001789:Blood Group Antigens; D015342:DNA Probes; D018076:DNA, Complementary; D012204:Rh-Hr Blood-Group System; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII