Responses of rat medullary dorsal horn neurons following intranasal noxious chemical stimulation: effects of stimulus intensity, duration, and interstimulus interval.
1. Most quantitative examinations of nociception are performed with thermal or mechanical stimuli. Because nociceptive processing mechanisms may depend on the modality of the stimuli, comparable studies on chemonociception are necessary. 2. We examined the activity of chemonociceptive medullary dorsal horn neurons in halothane-anesthetized rats. For controlled noxious chemical stimulation, defined CO2 pulses were applied to the nasal mucosa. The effects of stimulus intensity, duration, and interstimulus interval (ISI) were tested by performing three different CO2 stimulation protocols (see below). 3. The recorded neurons were characterized by intranasal and facial stimuli of different modalities. The cells received input from intranasal A delta- and/or C-fibers. All tested neurons also responded to other intranasally applied irritants, e.g., mustard oil. Furthermore, the units were sensitive to intranasal high-threshold mechanical stimulation and to facial mechanical stimulation. According to the properties of their facial mechanoreceptive fields, the units were classified as wide dynamic range (WDR) or nociceptive specific (NS) neurons. The majority of the cells also responded to facially applied noxious heat stimuli, so that most of the recorded neurons were found to be multimodal. Some of the neurons, in addition, had convergent input from primary afferents innervating the maxillary tooth pulps or the cornea and periorbital structures. 4. In the first stimulation protocol we presented four different CO2 concentrations (25, 50, 75, and 100%; stimulus duration 2 s). In total, each concentration was applied 10 times (2 trains of 5 stimuli). Stimulus response functions (SRFs) were computed with average responses to identical stimuli. All but 2 of the 23 tested neurons displayed enhanced responses after stimulation with increasing intensities. In general, WDR cells (n = 15) discharged more vigorously to the same CO2 concentration than NS cells (n = 8). WDR neurons discriminated more reliably between stimulus intensities in the low to moderate range (25-50% CO2) than NS cells. Both categories of neurons, however, discriminated equally well in the moderate- to high-intensity range (50-75% CO2). The discriminatory capacity of WDR and NS neurons was reduced in the highest concentration range (75-100% CO2). The proportion of NS neurons significantly discriminating between these intensities tended to be higher compared with WDR neurons when stimuli were applied with long ISIs (120 s). 5. To examine the effects of the duration of the ISI, identical test sequences were performed with ISIs of 30 and 120 s. (ABSTRACT TRUNCATED AT 400 WORDS)
Peppel
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P
Department of Physiology and Biocybernetics, University of Erlangen/Nürnberg, Germany.
Anton
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F
D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D001931:Brain Mapping; D001933:Brain Stem / Q000502:physiology; D002245:Carbon Dioxide; D002628:Chemoreceptor Cells / Q000502:physiology*; D005005:Ethmoid Sinus / Q000294:innervation; D005073:Evoked Potentials, Somatosensory / Q000502:physiology; D005145:Face / Q000294:innervation; D005727:Ganglia, Spinal / Q000502:physiology*; D008297:Male; D008465:Mechanoreceptors / Q000502:physiology; D009297:Nasal Mucosa / Q000294:innervation*; D009412:Nerve Fibers / Q000502:physiology; D009474:Neurons / Q000502:physiology; D009619:Nociceptors / Q000502:physiology*; D017288:Pain Threshold / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000294:innervation; D013116:Spinal Cord / Q000502:physiology*; D013823:Thermoreceptors / Q000502:physiology; D013697:Thermosensing / Q000502:physiology; D014276:Trigeminal Nerve / Q000502:physiology; D014278:Trigeminal Nuclei / Q000502:physiology
D002245:Carbon Dioxide