Rab effector domain peptides stimulate the release of neurotransmitter from cell cultured synapses.
The involvement of the small GTP-binding protein rab3A in synaptic transmission was tested by presynaptic microinjection of guanine nucleotides and peptides corresponding to the effector domain of rab3A. When GTP gamma S injection was paired with presynaptic action potentials, the frequency of MIPSCs was increased and the evoked synaptic current was reduced in magnitude. To more specifically manipulate rab proteins, peptides were microinjected. Injection of the peptide rab3AL(33-48) or rab3(29-48) stimulated an increase in the frequency of MIPSC with little effect on action potential evoked synaptic transmission supporting a role for rab proteins in regulating synaptic transmission.
Richmond
J
J
Signal Transduction Training Group, Iowa State University, Ames 50011.
Haydon
P G
PG
D000200:Action Potentials / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D004553:Electric Conductivity; D019204:GTP-Binding Proteins / Q000502:physiology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D008845:Microinjections; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D012908:Snails; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D020692:rab3 GTP-Binding Proteins
D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D010446:Peptide Fragments; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D019204:GTP-Binding Proteins; D020692:rab3 GTP-Binding Proteins