A novel kit for rapid detection of Vibrio cholerae O1.
We report on the development and testing of a novel, rapid, colorimetric immunodiagnostic kit, Cholera SMART, for direct detection of the presence of Vibrio cholerae O1 in clinical specimens. Unlike conventional culture methods requiring several days to complete, the Cholera SMART kit can be used directly in the field by untrained or minimally skilled personnel to detect V. cholerae O1 in less than 15 min, without cumbersome laboratory equipment. A total of 120 clinical and environmental bacterial strains, including both O1 and non-O1 serotypes of V. cholerae isolated from samples collected from a variety of geographical regions, were tested, and positive reactions were observed only with V. cholerae O1. Also, results of a field trial in Bangladesh, employing Cholera SMART, showed 100% specificity and 96% sensitivity compared with conventional culture methods. Another field trial, in Mexico, showed that Cholera SMART was 100% in agreement with a recently described coagglutination test when 108 stool specimens were tested.
Hasan
J A
JA
Department of Microbiology, University of Maryland, College Park 20742.
Huq
A
A
Tamplin
M L
ML
Siebeling
R J
RJ
Colwell
R R
RR
D001459:Bangladesh / Q000453:epidemiology; D002771:Cholera / Q000145:classification / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008800:Mexico / Q000453:epidemiology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012703:Serotyping
D011933:Reagent Kits, Diagnostic