The condensing effects of egg lecithin and cholesterol on triolein monolayers are inhibited by substitution of one saturated acyl chain in the triacylglycerol.
Previous work showed that the clearance from plasma of chylomicron-like emulsions injected intravenously was affected by the acyl chains of the constituent triacylglycerols. Compared with emulsions containing triolein (OOO) as the only triacylglycerol, clearances were decreased by a single saturated chain in emulsions containing 1,3-dioleoyl-2-stearoyl-sn-glycerol (OSO), 1,2-dioleoyl-3-stearoyl-sn-glycerol (OOS) or 1-stearoyl-2,3-dioleoyl-sn-glycerol (SOO). The differences in clearance may reflect physical differences at the oil-water interface related to chain interactions of the triacylglycerol structures with other lipid components. In the present work lipid monomolecular films at the air-water interface were used to establish the capacity of OOO to interact with the pure synthetic triacylglycerols OOS and SOO, and the capacity of OOS and SOO to co-exist in monolayers of lecithin and of cholesterol was compared with OOO. Substituting one oleoyl chain by a stearoyl chain induced a 20% condensation in monomolecular films of the pure triacylglycerols. Mixtures of OOO with either pure egg yolk phosphatidylcholine or cholesterol also showed substantial condensing effects. In contrast substituting one oleoyl chain by a stearoyl chain substantially lessened the condensing effects. At surface pressures above the collapse pressure of the pure triacylglycerols, substantially more OOO than OOS or SOO was retained in mixed monolayers with phosphatidylcholine. These differences could underlie the effects on metabolism of saturated chains in emulsion triacylglycerols.
Redgrave
T G
TG
Department of Physiology, University of Western Australia, Nedlands.
Ivanova
M G
MG
Verger
R
R
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002784:Cholesterol / Q000494:pharmacology*; D004655:Emulsions; D019301:Oleic Acid; D009829:Oleic Acids / Q000737:chemistry; D010713:Phosphatidylcholines / Q000494:pharmacology*; D013229:Stearic Acids / Q000737:chemistry; D014280:Triglycerides / Q000737:chemistry*; D014304:Triolein / Q000737:chemistry*
D004655:Emulsions; D009829:Oleic Acids; D010713:Phosphatidylcholines; D013229:Stearic Acids; D014280:Triglycerides; D014304:Triolein; D019301:Oleic Acid; C031183:stearic acid; D002784:Cholesterol