The monosialoganglioside GM1 dose-dependently reduces regional cerebral metabolic rates for glucose in awake rats.
Using the quantitative autoradiographic [14C]2-deoxyglucose technique, regional cerebral metabolic rates for glucose (rCMRglc) were measured in awake male Fischer-344 rats at 1, 2, 3, 4 and 6 h after administration of GM1 30 mg/kg and at 3 h after GM1 150 or 300 mg/kg. GM1 is a natural compound that is able to prevent neuron degeneration induced by exposure to excitatory amino acids in vitro and by ischemia or neurotoxins in vivo. GM1 30 mg/kg, a dose very effective in preventing excitatory amino acid-induced neurotoxicity, produced minimal rCMRglc change over a 6 h period. GM1 150 and 300 mg/kg reduced rCMRglc, in 14 (31%) and in 29 (64%) brain regions, respectively. Maximal metabolic effects occurred in hippocampal areas which possess, in specific subfields, the highest brain concentrations of different excitatory amino acid receptor subtypes. This finding suggests an effect by GM1 on postreceptor mechanisms common to different excitatory amino acids.
Freo
U
U
Clinica delle Malattie Nervose e Mentali, Padova, Italy.
Dam
M
M
Pizzolato
G
G
Pietrini
P
P
Soncrant
T T
TT
Battistin
L
L
D000818:Animals; D001224:Aspartic Acid / Q000502:physiology*; D001667:Binding, Competitive / Q000502:physiology; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D005971:Glutamates / Q000502:physiology*; D018698:Glutamic Acid; D008297:Male; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors; D009474:Neurons / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D005971:Glutamates; D001224:Aspartic Acid; D005677:G(M1) Ganglioside; D018698:Glutamic Acid; D016202:N-Methylaspartate; D005947:Glucose