[Reactivation of Escherichia coli inactivated by ultraviolet light by cell extracts of propionic acid bacteria].
For the first time reactivation of cell extract of three strains of Propionibacterium shermanii in UV inactivated not filament-forming strain Escherichia colli AB 1157 is shown. Reactivation was demonstrated in preincubated and postincubated test-culture and increased as survival of E. coli decreased in a range 1.8-0.006%. The factor (factors) of defense is dialysable, thermolabile and is present as in a fraction of nucleoproteins and nucleic acids so in a fraction of soluble proteins. The extracts were inactivated by incubation with proteinase K and trypsin, partly decreased activity by incubation with alpha-amylase and selected nuclease but not with lipase. Polypeptide nature of reactivating factor is supposed.
Vorob'eva
L I
LI
Nikitenko
G V
GV
Khodzhaev
E Iu
EIu
Ponomareva
G M
GM
D002244:Carbon; D003470:Culture Media; D003956:Dialysis; D004798:Enzymes / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology* / Q000528:radiation effects*; D011424:Propionibacterium / Q000378:metabolism*; D013045:Species Specificity; D013696:Temperature; D014466:Ultraviolet Rays
D003470:Culture Media; D004798:Enzymes; D002244:Carbon
Reaktivatsiia inaktivirovannykh ul'travioletovym svetom Escherichia coli kletochnymi ékstraktami propionovo-kislykh bakteriĭ.