The T cell receptor V beta repertoire in HIV-1 infection and disease.
Viral superantigens (SAg) were shown in mice to induce anergy and deletion of T cells bearing specific T cell receptor V beta subsets, these perturbations being mainly restricted to CD4+ T cells. In accordance with this model, a putative HIV-associated SAg could contribute to the pathogenesis of HIV-1 infection and AIDS. To reveal the presence of this putative molecule, three study protocols were designed that relied on the fact that similarity of the expressed V beta repertoire of a given pair of individuals is proportional to the relative likeness of their MHC background: (1) by using a quantitative PCR technique that allows simultaneous typing of 24 V beta families, the V beta repertoires of HIV-discordant monozygotic twins were compared; (2) the V beta repertoire found in lymph nodes of HIV-infected subjects was contrasted to that found in peripheral blood of the same individuals; (3) the V beta repertoire of a cohort of HIV-infected mothers was compared with that of their HIV-infected and uninfected children. Results from these approaches revealed that significant perturbations of the TCR V beta repertoire were taking place in HIV-infected subjects, and that these alterations were restricted to T cells expressing specific V beta s. These results are consistent with the presence of an HIV-associated SAg in HIV-1 infection.
Soudeyns
H
H
Laboratoire d'Immunologie, Institut de Recherches Cliniques de Montreal, Quebec, Canada.
Rebai
N
N
Pantaleo
G P
GP
Ciurli
C
C
Boghossian
T
T
Sékaly
R P
RP
Fauci
A S
AS
D000328:Adult; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D017209:Apoptosis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D015331:Cohort Studies; D004200:Diseases in Twins; D005260:Female; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D015488:HIV Antigens / Q000276:immunology*; D015658:HIV Infections / Q000151:congenital / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D006681:HLA-D Antigens / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance; D007231:Infant, Newborn; D009052:Leukemia Virus, Murine / Q000276:immunology / Q000472:pathogenicity; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation; D051379:Mice; D008954:Models, Biological; D016183:Murine Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D014430:Twins, Monozygotic
D000942:Antigens, Bacterial; D000956:Antigens, Viral; D015488:HIV Antigens; D006681:HLA-D Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta