Adherence of non-enteropathogenic Escherichia coli to HeLa cells.
Three hundred and nine strains of Escherichia coli isolated from infants and children with diarrhoea but not belonging to any recognised classes of diarrhoeagenic E. coli were investigated for their ability to adhere to HeLa cells in the presence of D-mannose. An enteroadherent-aggregative pattern (EAgg) was observed in 32.03%, localised adherence (LA) in 4.5%, diffuse adherence (DA) in 5.8%, and LA/DA and EAgg/LA in 1.9% and 1.2% of the isolates respectively. The results obtained with 100 control isolates were: EAgg 17%, LA 2%, DA 2%, LA/DA 2%, EAgg/LA 6% and DA/EAgg 1%. No adherence was manifested by 168 (54.36%) of 309 diarrhoeal isolates and 70% of the 100 control isolates. The results of this study showed that amongst non-enteropathogenic E. coli, strains exhibiting the EAgg pattern are significantly associated with diarrhoea (p < 0.005). Most of these strains showed a pattern of multiple drug resistance.
Bouzari
S
S
Molecular Biology Unit, Pasteur Institute, Tehran, Iran.
Jafari
A
A
Farhoudi-Moghaddam
A A
AA
Shokouhi
F
F
Parsi
M
M
D001422:Bacterial Adhesion; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology*; D006367:HeLa Cells; D006801:Humans; D007223:Infant; D008358:Mannose
D008358:Mannose