A gene homologous to that encoding UDP galactose-4-epimerase is inducible by xylose in the yeast Pachysolen tannophilus.
Using DNA sequencing, we have identified a fragment of genomic DNA from Pachysolen tannophilus that is homologous to the GAL10 gene in yeasts and galE gene in bacteria, both encoding the UDP galactose-4-epimerase (EC 5.1.3.2). This gene (designated PtGAL10) is equally efficiently inducible by D-galactose (a hexose) and D-xylose (a pentose). The encoded protein shows the highest similarity to the homologous Kluyveromyces lactis protein. This includes the N-terminal domain that is not present in homologous polypeptides from other organisms.
Skrzypek
M
M
Department of Biochemistry, University of Kentucky, Lexington 40506.
Maleszka
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015870:Gene Expression / Q000187:drug effects; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D051381:Rats; D004718:Saccharomycetales / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D014534:UDPglucose 4-Epimerase / Q000235:genetics*; D014994:Xylose / Q000494:pharmacology*
D009838:Oligodeoxyribonucleotides; D014994:Xylose; D014534:UDPglucose 4-Epimerase