Plasma levels of the anti-HIV drug 3'-azido-2',3'-dideoxythymidine (AZT): determination by RIA and HPLC.
The radioimmunoassay (RIA) with two commercial kits and the high pressure liquid chromatography (HPLC) method were employed to test human and dog blood plasma levels of AZT following oral and intravenous administration of AZT preparations Retrovir and Azitidin. A comparison of results obtained by the two RIA kits showed a good correlation. A weaker correlation was established in comparing the results obtained by RIA with those obtained by HPLC. Because of its reliability, rapid availability of results and the price, RIA is more advantageous than HPLC for routine use in monitoring the therapy as well as in determining pharmacokinetic parameters of AZT.
Földes
O
O
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava.
Uherová
P
P
Mayer
V
V
D000284:Administration, Oral; D000328:Adult; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D016903:Drug Monitoring; D015658:HIV Infections / Q000097:blood* / Q000188:drug therapy; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D015215:Zidovudine / Q000008:administration & dosage / Q000097:blood*
D011933:Reagent Kits, Diagnostic; D015215:Zidovudine