title: Arohynapenes A and B, new anticoccidial agents produced by Penicillium sp. Taxonomy, fermentation, and structure elucidation.
abstract: Penicillium sp. FO-2295, a water isolate, was found to produce a series of new anticoccidial compounds. Two active compounds, designated arohynapenes A and B, were isolated from the fermentation broth of the producing strain by solvent extration and preparative HPLC. Arohynapene A was deduced to be (2E,4E)-5-(5-hydroxy-2,6,8-trimethyl-5,6,7,8-tetrahydronaphtale ne)-2,4- pentadienoic acid, and arohynapene B was (2E,4E)-5-(2-hydroxymethyl-6,8-dimethyl-5,6,7,8-tetrahydronapht alene)-2,4- pentadienoic acid. Arohynapenes inhibited the growth of Eimeria tenella in an in vitro assay using BHK-21 cells as a host. No schizont in the cells was observed at concentrations ranging above 35.0 microM and 7.0 microM for arohynapenes A and B, respectively.
authors.affiliation: Research Center for Biological Function, Kitasato Institute, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Masuma
authors.forename: N
authors.initials: N
authors.lastname: Tabata
authors.forename: H
authors.initials: H
authors.lastname: Tomoda
authors.forename: K
authors.initials: K
authors.lastname: Haneda
authors.forename: Y
authors.initials: Y
authors.lastname: Iwai
authors.forename: S
authors.initials: S
authors.lastname: Omura
mesh_terms: D003049:Coccidiostats / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008826:Microbial Sensitivity Tests; D010407:Penicillium / Q000378:metabolism*; D013764:Tetrahydronaphthalenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D003049:Coccidiostats; D013764:Tetrahydronaphthalenes; C086152:arohynapene A; C086153:arohynapene B