Preparation of cyclic 2',3'-monophosphates of oligoadenylates (A2'p)nA > p and A3'p(A2'p)n-1A > p.
The action of the guanylyl-preferring RNase from Bacillus intermedius (binase) on a mixture of oligoadenylates with randomly distributed 2'-5' and 3'-5' internucleotide bonds [(A2'/3'p)n] under conditions sufficient for complete hydrolysis of poly(A) results in a mixture containing a single circular oligoadenylate and two series of linear oligoadenylates ending in cyclic 2',3'-phosphate. Individual compounds formed upon digestion of (A2'/3'p)n with binase have been isolated. Their structure was determined on the basis of their chemical and enzymatic conversions and confirmed by 1H-, 13C- and 31P-NMR spectra. According to these data, the circular triadenylate contains one 2'-5' and two 3'-5' internucleotide bonds, linear oligoadenylates of one series contain exclusively 2'-5' internucleotide bonds [(A2'p)nA > p], while each compound of the other series contains a single 3'-5' internucleotide bond connecting the 5'-ultimate nucleotide residue with the penultimate one [A3'p(A2'p)n-1A > p]. The incubation of compounds of the former series A3' p(A2'p)n > p at pH 1.0 and the subsequent action of phosphatase results in successive formation of compounds of two other new series: A3'p(A2'p)nA2'(3')p and A3'p(A2'p)nA.
Budowsky
E I
EI
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Science, Moscow.
Kayushina
E N
EN
Tarasov
A K
AK
Orlenko
S A
SA
Cherkasov
I A
IA
Gavrilov
A E
AE
Strelenko
Y A
YA
D000227:Adenine Nucleotides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology; D004722:Endoribonucleases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D011061:Poly A / Q000378:metabolism
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D011061:Poly A; C023505:2',5'-oligoadenylate; C010565:2',3'-cyclic AMP; D004722:Endoribonucleases; C022543:ribonuclease T(2)