title: Hepatic oxygen consumption and cytochrome P450 activity in experimental faecal peritonitis.
abstract: OBJECTIVE
  To study hepatic oxygen consumption and cytochrome P450 activity in pigs with septic shock induced by faecal peritonitis.
  
  
  METHODS
  Controlled experimental study.
  
  
  METHODS
  12 pigs weighing 19-27 kg.
  
  
  METHODS
  The animals were divided into a control group (n = 6) and a peritonitis group (n = 6). Peritonitis was induced by intraperitoneal instillation of a standard amount of autologous faeces. The animals were then observed for 300 minutes. Liver biopsy specimens were taken at 0 and 300 minutes.
  
  
  METHODS
  Hepatic oxygen delivery (DO2) and consumption (VO2). Cytochrome P450 activity was studied by measuring O- and N-demethylation of codeine at 0 and 300 minutes.
  
  
  RESULTS
  Hepatic DO2 was reduced, whereas VO2 was increased during sepsis. There were no significant changes in the N- and O-demethylation of codeine.
  
  
  CONCLUSIONS
  Hepatic VO2 did increase during sepsis, possibly because of the increased metabolic demand. Cytochrome P450 activity was unaffected by the septic challenge.
authors.affiliation: Department of Surgery, University Hospital, Uppsala, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Rasmussen
authors.forename: A
authors.initials: A
authors.lastname: Rane
authors.forename: U
authors.initials: U
authors.lastname: Haglund
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure; D001810:Blood Volume; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005243:Feces; D005260:Female; D006503:Hepatic Veins / Q000378:metabolism / Q000503:physiopathology; D008099:Liver / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D010101:Oxygen Consumption; D010538:Peritonitis / Q000378:metabolism* / Q000503:physiopathology; D011169:Portal Vein / Q000378:metabolism / Q000503:physiopathology; D011651:Pulmonary Artery / Q000378:metabolism / Q000503:physiopathology; D011897:Random Allocation; D013552:Swine; D014655:Vascular Resistance
chemical_list: D003577:Cytochrome P-450 Enzyme System