Human platelet antigen-1 (Zw) typing using PCR-RFLP.
The agreement between human platelet antigen-1 typing with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and typing with a serological ELISA method was evaluated. A total of 82 individuals were typed and an absolute correlation was found between the two typing methods. The PCR-RFLP typing method could be clinically useful in a number of immunologically mediated platelet disorders, characterized by severe thrombocytopenia and in early prenatal diagnosis of neonatal alloimmune thrombocytopenia, in which serological methods are difficult to apply because of their dependency on access to platelets and access to well-characterized anti-HPA-1b antisera.
Andersen
B R
BR
Department of Clinical Immunology, State University Hospital, Rigshospitalet, Copenhagen, Denmark.
Georgsen
J
J
Madsen
H O
HO
Taaning
E
E
Grunnet
N
N
Svejgaard
A
A
D016824:Antigens, Human Platelet / Q000032:analysis*; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D039661:Integrin beta3; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3