Development of an isotope-selective high-performance liquid chromatography detector using chemical-reaction-interface mass spectrometry: application to deuterated cortisol metabolites in urine.
An isotope-selective detector for HPLC based on the particle-beam-interface and the chemical-reaction-interface mass spectrometer (CRIMS) principle is described. This paper focuses on the selective detection of deuterium-labeled analytes. The CRIMS product HD is detected as has been previously described for GC-CRIMS. The analytical performance was not affected by analyte structure or solvent composition. Deuterium detection was linear from 20 ng to 490 ng using 2H3-labeled cortisol. Based on this method, the fractional abundance of three labeled metabolites of cortisol were determined in the urine of a patient infused with tracer amounts of deuterated cortisol.
Teffera
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George Washington University Medical Center, Department of Pharmacology, Washington, DC 20037.
Abramson
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FP
McLean
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Vestal
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M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003903:Deuterium; D006801:Humans; D006854:Hydrocortisone / Q000652:urine*; D013058:Mass Spectrometry / Q000379:methods*; D011278:Pregnanes / Q000652:urine; D013760:Tetrahydrocortisol / Q000652:urine; D013761:Tetrahydrocortisone / Q000652:urine; D014316:Tritium
D011278:Pregnanes; D014316:Tritium; C003545:cortolone; D013761:Tetrahydrocortisone; D013760:Tetrahydrocortisol; D003903:Deuterium; D006854:Hydrocortisone