[Cathepsin D expression in primary breast cancer. Comparison of immunohistochemical and biochemical results].
Cathepsin D expression was immunohistochemically tested in 126 formalin-fixed and paraffin-embedded tissues from primary breast cancers. Specific staining was noted in the cytoplasm of tumor cells in 43% of tumors. In 44% of cases cathepsin D was detected in tumor stroma. A total of 63% of the tissue samples stained positive for cathepsin D (tumor and/or stromal staining). In all cases the cathepsin D level was measured in tumor cytosol by radioimmunoassay. Cytosols were considered positive when cathepsin D concentrations of over 40 pmol/mg cell protein were found. Comparison of immunohistochemical and biochemical detection of cathepsin D revealed a 76% concordance rate. Univariate analysis showed a significant correlation of cathepsin D expression with axillary node involvement; no correlation was found with tumor size, tumor grade, steroid receptor status or age of patients. Our results demonstrate a close agreement between the results of biochemical and of immunohistochemical cathepsin D detection.
Göhring
U J
UJ
Klinik und Poliklinik für Frauenheilkunde und Geburtschilfe, Universität zu Köln.
Ingenhorst
A
A
Crombach
G
G
Scharl
A
A
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology*; D002402:Cathepsin D / Q000032:analysis*; D003600:Cytosol / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D015592:Immunoradiometric Assay; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis
D014408:Biomarkers, Tumor; D002402:Cathepsin D
Kathepsin-D-Expression im primären Mammakarzinom. Vergleich immunohistochemischer und biochemischer Ergebnisse.