Cloning and expression analysis of a viroid-induced peroxidase from tomato plants.
Differential hybridization was used to detect transcripts induced both by ethylene and by viroid infection in tomato plants. A cDNA clone encoding a putative peroxidase was isolated and characterized. DNA sequencing revealed high homology with a lignin-peroxidase from tobacco. Northern blot analysis showed specific induction of this peroxidase gene in viroid-infected plants. An increase in the level of mRNA accumulation is obtained by ethylene treatment, reinforcing the idea that ethylene is a mediator in the response of tomato plants to viroid infection.
Vera
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Departament of Biotechnology, Universidad Politécnia de Valencia, Spain.
Tornero
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Conejero
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004790:Enzyme Induction; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014675:Vegetables / Q000201:enzymology* / Q000382:microbiology; D014772:Viroids / Q000502:physiology*
D018076:DNA, Complementary; D010544:Peroxidases