Molecular cloning of infectious ecotropic murine leukemia virus AK7 from an emv-14-positive AKXL-5 mouse and the resistance of AK7 to recognition by cytotoxic T lymphocytes.
The AKXL-5 recombinant inbred mouse strain is positive for the endogenous ecotropic murine leukemia virus emv-14, the only emv present in its germ line. emv-14 is of particular interest because spleen cells expressing emv-14 virus escape recognition by anti-AKR/Gross virus-specific cytotoxic T lymphocytes. We report here the isolation and characterization of a replication-competent emv clone, pAK7, derived from an AKXL-5 mouse. This clone is novel in that it encodes a variant ecotropic murine leukemia virus that, when expressed in SC.Kb target cells, fails to be recognized efficiently by anti-AKR/Gross virus cytotoxic T lymphocytes. The pAK7 clone can therefore be used to further probe mechanisms of escape from cell-mediated immunity.
White
H D
HD
Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03756.
Green
W R
WR
Giné
N R
NR
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005347:Fibroblasts; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000382:microbiology; D014771:Virion / Q000235:genetics / Q000502:physiology; D016662:Virus Integration; D014779:Virus Replication
D004279:DNA, Viral