title: A rapid and sensitive hemolysis neutralization assay for palytoxin.
abstract: The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37 degrees C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of Palythoa tuberculosa.
authors.affiliation: Hawaii Biotechnology Group, Inc., Aiea 96701.
authors.forename: G S
authors.initials: GS
authors.lastname: Bignami
mesh_terms: D000178:Acrylamides; D000818:Animals; D000911:Antibodies, Monoclonal; D003064:Cnidarian Venoms / Q000032:analysis*; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006461:Hemolysis; D007124:Immunoenzyme Techniques; D051379:Mice; D009500:Neutralization Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D000095702:Polyether Toxins
chemical_list: D000178:Acrylamides; D000911:Antibodies, Monoclonal; D003064:Cnidarian Venoms; C010272:palytoxin; D000095702:Polyether Toxins