The equine herpesvirus type 1 glycoprotein homologous to herpes simplex virus type 1 glycoprotein M is a major constituent of the virus particle.
Glycoprotein 45 is a major envelope glycoprotein of equine herpesvirus type 1. The gene encoding this protein is located between map units 0.615 and 0.636 on the virus genome and evidence has suggested that it is encoded by gene 52, one of four genes within this region. Using PCR we have amplified gene 52 and subsequently cloned it into a mammalian expression vector under the control of the human cytomegalovirus immediate early gene promoter. The gene was expressed in COS-7 cells and its product was detected by immunofluorescence and Western blotting. The results indicate that glycoprotein 45 is encoded by gene 52, and that it is the homologue of herpes simplex virus type 1 glycoprotein M.
Pilling
A
A
Department of Microbiology, University of Leeds, U.K.
Davison
A J
AJ
Telford
E A
EA
Meredith
D M
DM
D001483:Base Sequence; D002478:Cells, Cultured; D005814:Genes, Viral; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D018259:Herpesvirus 1, Human / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins