Restriction fragment length polymorphisms (RFLPs) of medicinal plants and crude drugs. I. RFLP probes allow clear identification of Duboisia interspecific hybrid genotypes in both fresh and dried tissues.
A simple and efficient method for identification of Duboisia leichhardtii F. Muell, D. myoporoides R. BR. and their interspecific hybrid was established by analyzing restriction fragment length polymorphism (RFLP) profiles using non-radioactive rice ribosomal DNA as a probe. This procedure was shown to be applicable to both fresh and dried samples, and also suitable for detection of somatic hybrid at the callus stage after protoplast fusion.
Mizukami
H
H
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Ohbayashi
K
K
Kitamura
Y
Y
Ikenaga
T
T
D004247:DNA / Q000032:analysis; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006824:Hybridization, Genetic; D012275:Oryza / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011523:Protoplasts; D013045:Species Specificity
D015342:DNA Probes; D004275:DNA, Ribosomal; D004365:Drugs, Chinese Herbal; D004247:DNA