title: Sequence analysis of the rDNA spacer of Paracentrotus lividus and observations about pre-rRNA processing. NTS sequence of Paracentrotus lividus rDNA.
abstract: We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchin Paracentrotus lividus. This region is about 3.8 Kb long. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S rRNA precursor while has the same 5' terminus as the 32S primary precursor, also in developmental stages characterized by a low rate of rRNA synthesis.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Cantone
authors.forename: R
authors.initials: R
authors.lastname: Barbieri
authors.forename: G
authors.initials: G
authors.lastname: Duro
authors.forename: V
authors.initials: V
authors.lastname: Izzo
authors.forename: G
authors.initials: G
authors.lastname: Giudice
mesh_terms: D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D015183:Restriction Mapping; D012617:Sea Urchins / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004275:DNA, Ribosomal; D012322:RNA Precursors