The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast.
The nucleotide sequence of a 19,000 base pair region from the left arm of chromosome XI of Saccharomyces cerevisiae has been determined and analysed. It covers the HAP4-GFA1-LAP4 loci already described. As expected HAP4, GFA1 and LAP4 genes have been found and six new open reading frames (ORFs) with a coding capacity of more than 100 amino acid residues have been identified. One of them (YKL461) shows a high degree of identity with an aspartate aminotransferase gene. This raises the question of a second aspartate aminotransferase gene in yeast. A second ORF (YKL462) shows features compatible with a membranous localization. The other ORFs do not show a similarity with any known gene. A member of the highly repetitive 'CAT' DNA sequence is present.
Chéret
G
G
Institut Curie, Centre Universitaire, Orsay, France.
Pallier
C
C
Valens
M
M
Diagnan-Fornier
B
B
Fukuhara
H
H
Bolotin-Fukuhara
M
M
Sor
F
F
D001219:Aspartate Aminotransferases / Q000235:genetics*; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*
D004271:DNA, Fungal; D001219:Aspartate Aminotransferases