Expression of sialyl Lewis(x) antigen on human T cells.
Sialyl Lewis(x) (sLe(x)) antigen, which has been introduced as tumor-associated cell surface carbohydrates, was demonstrated to be expressed on a subpopulation of human CD4+ and CD8+ T cells. It was also demonstrated that the majority of gamma delta T cells as well as natural killer cells expressed a high level of sLe(x) antigen. The sLe(x) expression on T cells were up-regulated by activation with various T cell stimulants. The sLe(x)+ CD4+ T cells were enriched in CD4+CD45RO+ memory type of T cells but not in CD4+CD45RA+ T cells. Moreover, sLe(x)+ CD4+ T cells were demonstrated to show higher proliferative responses to T cell stimulants such as CD4+CD45RO+ T cells. These results initially clarified the expression of sLe(x) carbohydrate on human T cells and indicated the important role of sLe(x)-expressing T cells in immune responses.
Ohta
S
S
Tokyo Research Laboratories, Kyowa Hakko Kogo Co., Ltd., Japan.
Hanai
N
N
Habu
S
S
Nishimura
T
T
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005434:Flow Cytometry; D006801:Humans; D016256:Lewis X Antigen / Q000032:analysis*; D008213:Lymphocyte Activation / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D016256:Lewis X Antigen