title: Expression of homeobox genes Msx-1 (Hox-7) and Msx-2 (Hox-8) during cardiac development in the chick.
abstract: The vertebrate homeobox genes Msx-1 and Msx-2 are related to the Drosophila msh gene and are expressed in a variety of tissues during embryogenesis. We have examined their expression by in situ hybridisation during critical stages of cardiac development in the chick from stages 15+ to 37. Msx-1 expression is apparent in a number of non-myocardial cell populations, including cells undergoing an epithelial to mesenchymal transformation in the atrioventricular and the outflow tract regions that play an integral role in heart septation and valve formation. Msx-2 expression is restricted to a distinct subpopulation of myocardial cells that, in later stages, coincides morphologically with the cardiac conduction system. The timing of Msx-2 expression suggests that it plays a role in conduction system tissue formation and that it identifies precursor cells of this specialised myocardium. The pattern of Msx-2 expression is discussed with reference to current models of conduction tissue development.
authors.affiliation: Department of Cardiothoracic Surgery, National Heart and Lung Institute, London, United Kingdom.
authors.forename: P S
authors.initials: PS
authors.lastname: Chan-Thomas
authors.forename: R P
authors.initials: RP
authors.lastname: Thompson
authors.forename: B
authors.initials: B
authors.lastname: Robert
authors.forename: M H
authors.initials: MH
authors.lastname: Yacoub
authors.forename: P J
authors.initials: PJ
authors.lastname: Barton
mesh_terms: D000818:Animals; D030161:Avian Proteins; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005801:Genes, Homeobox / Q000235:genetics* / Q000502:physiology; D006321:Heart / Q000196:embryology*; D018398:Homeodomain Proteins; D017403:In Situ Hybridization; D051957:MSX1 Transcription Factor; D009206:Myocardium / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor; C084119:MSX2 protein; C071559:Hbox 7 protein, Gallus gallus