The type III-9 repeat of human fibronectin is encoded by a single exon which is not alternatively spliced.
Type III homologies of human fibronectin are generally encoded by two exons, with the exception of the ED-A and ED-B repeats which are encoded by a single exon undergoing alternative splicing. We report that also the type III-9 homology is encoded by a single exon. Further more, RT-PCR analysis, performed on mRNA purified from fetal and adult tissues and from normal and tumor-derived cell types, showed that the III-9 region is not undergoing alternative splicing in all samples tested.
Gardella
R
R
Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy.
Barlati
S
S
Ferraboli
S
S
Colombi
M
M
D000328:Adult; D017398:Alternative Splicing; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005091:Exons; D005260:Female; D005333:Fetus; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D004273:DNA, Neoplasm; D005353:Fibronectins; D004247:DNA