Purification and characterization of a 100 kDa glycyrrhizin (GL)-binding protein (gp100) as an effective phosphate acceptor for CK-II and the effect of GL on the phosphorylation of gp100 by CK-II in vitro.
By means of successive GL-affinity and Mono S column chromatographies (HPLC), a 100 kDa GL-binding protein (gp100) was purified from the partially purified CK-II fraction of EAT cells as an effective phosphate acceptor for CK-II. It was found that (i) gp100 (pI 9.0) is copurified with CK-II, Hsp-90 and p34 or p70; (ii) gp100 cross-reacts with anti-human GR; (iii) phosphorylation of gp100 by CK-II is significantly stimulated by 1 microM GL or 0.3 microM oGA; and (iv) GL as well as DEX inhibit it at doses above 3 microM. Data are provided to suggest that the GL-induced selective inhibition of the CK-II catalyzed phosphorylation of gp100 may be involved in the anti-inflammatory effects of GL.
Ohtsuki
K
K
Department of Bioscience, Kitasato University School of Hygienic Sciences, Sagamihara, Japan.
Oh-Ishi
M
M
Karino
A
A
Kanekatsu
M
M
Shamsa
F
F
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D047390:Casein Kinase II; D002645:Chickens; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003907:Dexamethasone / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D019695:Glycyrrhizic Acid; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000302:isolation & purification / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C490097:Hsp90b1 protein, mouse; C086010:glycyrrhizin-binding proteins; C065139:olean-11,13(18)-diene-3,30-diol dihemiphthalate, disodium salt; D019695:Glycyrrhizic Acid; D003907:Dexamethasone; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D006034:Glycyrrhetinic Acid