title: Linoleic acid controls neonatal tissue-specific stearoyl-CoA desaturase mRNA levels.
abstract: The mouse genome contains two stearoyl-CoA desaturase (SCD) structural genes (SCD1 and SCD2) that are expressed in a tissue-specific manner. Brain SCD2 mRNA levels are about 2-fold higher in pups nursed by mothers fed a control diet (5% corn oil (CO), (essential fatty acid (EFA) adequate)), compared with brain SCD2 mRNA levels in pups nursed by mothers fed an EFA-deficient (EFAD) diet (5% coconut oil (COCO)). In contrast to brain, control pup hepatic SCD1 mRNA levels are reduced to < 1.0% of the EFAD pup hepatic SCD1 mRNA levels. EFA status does not alter SCD1 or SCD2 transcription initiation sites. CCAAT/enhancer-binding proteins (C/EBP) have been implicated in the transcriptional control of key genes in energy metabolism. Both the SCD1 and SCD2 gene promoters contain C/EBP transcription factor consensus-binding sites. Neonatal mouse liver expresses C/EBP-alpha, C/EBP-beta and C/EBP-delta mRNAs. In contrast neonatal mouse brain expresses high levels of C/EBP-delta, but little C/EBP-alpha or C/EBP-beta mRNA. EFA intake has no effect on tissue-specific C/EBP isoform mRNA levels suggesting that C/EBP isoform function is controlled at the translational or post-translational level.
authors.affiliation: Ohio State University Biochemistry Program, Columbus 43210.
authors.forename: J W
authors.initials: JW
authors.lastname: DeWille
authors.forename: S J
authors.initials: SJ
authors.lastname: Farmer
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005260:Female; D005796:Genes; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D013230:Stearoyl-CoA Desaturase / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004042:Dietary Fats, Unsaturated; D008041:Linoleic Acids; D012333:RNA, Messenger; D019787:Linoleic Acid; D013230:Stearoyl-CoA Desaturase