Modification of Ca(2+)-sensitivity of Ca(2+)-activated Cl- channel by vasopressin and cholera toxin.
The apical membrane of renal epithelium (A6) had a Ca(2+)-activated, outwardly rectified chloride channel with the single channel conductance of 3 pS for outward current and 0.5 pS for inward current. The outward rectification was dependent on the cytosolic Ca2+ concentration. Vasopressin (arginine vasopressin, AVP) and cholera toxin (CTX) increased the single channel conductance for inward current through an increase in the Ca(2+)-sensitivity about 100-fold.
Marunaka
Y
Y
Hospital for Sick Children Research Institute, University of Toronto, Faculty of Medicine, Ontario, Canada.
D000663:Amphibians; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D018118:Chloride Channels / Q000187:drug effects* / Q000378:metabolism; D002772:Cholera Toxin / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D004553:Electric Conductivity; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism
D018118:Chloride Channels; D001127:Arginine Vasopressin; D002772:Cholera Toxin; D019204:GTP-Binding Proteins; D002118:Calcium