Salivary deoxyribonuclease I polymorphism separated by polyacrylamide gel-isoelectric focusing and detected by the dried agarose film overlay method.
Isoelectric focusing of whole saliva samples on polyacrylamide gels (pH 3.5-5), followed by dried agarose film overlay detection, was employed to determine the type of salivary deoxyribonuclease I (DNase I). Since this detection method had not only a high sensitivity, but also a high band resolution, it was possible to determine DNase I types from saliva samples of 2-5 microL. Pretreatment of saliva samples with neuraminidase simplified the isozyme pattern and enhanced the sensitivity. The DNase I types in all 30 saliva samples showed a good correlation with the types found in the corresponding blood, semen, and urine samples. This preliminary trial indicates that DNase I typing from saliva samples is a new and promising method for individualization of casework samples in the field of forensic biology.
Tenjo
E
E
Department of Legal Medicine, Fukui Medical School, Japan.
Sawazaki
K
K
Yasuda
T
T
Nadano
D
D
Takeshita
H
H
Iida
R
R
Kishi
K
K
D003850:Deoxyribonuclease I / Q000032:analysis* / Q000235:genetics*; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D011110:Polymorphism, Genetic; D012463:Saliva / Q000201:enzymology*; D012661:Semen / Q000201:enzymology; D012680:Sensitivity and Specificity; D012685:Sepharose
D012685:Sepharose; D003850:Deoxyribonuclease I