title: Differential expression of six glutamine synthetase genes in Zea mays.
abstract: The maize genome has been shown to contain six glutamine synthetase (GS) genes with at least four different expression patterns. Noncoding 3' gene-specific probes were constructed from all six GS cDNA clones and used to examine transcript levels in selected organs by RNA gel blot hybridization experiments. The transcript of the single putative chloroplastic GS2 gene was found to accumulate primarily in green tissues, whereas the transcripts of the five putative GS1 genes were shown to accumulate preferentially in roots. The specific patterns of transcript accumulation were quite distinct for the five GS1 genes, with the exception of two closely related genes.
authors.affiliation: Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108-1095.
authors.forename: M G
authors.initials: MG
authors.lastname: Li
authors.forename: R
authors.initials: R
authors.lastname: Villemur
authors.forename: P J
authors.initials: PJ
authors.lastname: Hussey
authors.forename: C D
authors.initials: CD
authors.lastname: Silflow
authors.forename: J S
authors.initials: JS
authors.lastname: Gantt
authors.forename: D P
authors.initials: DP
authors.lastname: Snustad
mesh_terms: D002451:Cell Compartmentation; D002736:Chloroplasts / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D015723:Gene Library; D017343:Genes, Plant; D016678:Genome; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis / Q000235:genetics*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D011061:Poly A / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014018:Tissue Distribution; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D011061:Poly A; D005974:Glutamate-Ammonia Ligase