title: Deletion of highly conserved C-terminal sequences in the Kv1 K+ channel sub-family does not prevent expression of currents with wild-type characteristics.
abstract: The C-terminal regions of Kv1 K+ channels show little conservation between isoforms except for the last four C-terminal residues, (E/L)TDV, which are well conserved from Drosophila to man. Deletions of the 4, 16, and 57 C-terminal residues of the human Kv1.5 channel did not affect whole cell current amplitude, midpoint of activation, degree of inactivation, or activation kinetics following expression in mouse L-cells. Similar results were obtained with the rat Kv1.1 channel. Therefore, the conserved (E/L)TDV motif, and most of the C-terminal amino acids, are not required for Kv1 channel expression.
authors.affiliation: Department of Molecular Physiology, Vanderbilt Medical School, Nashville, TN 37232.
authors.forename: V N
authors.initials: VN
authors.lastname: Uebele
authors.forename: S W
authors.initials: SW
authors.lastname: Yeola
authors.forename: D J
authors.initials: DJ
authors.lastname: Snyders
authors.forename: M M
authors.initials: MM
authors.lastname: Tamkun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D004330:Drosophila; D006801:Humans; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D015221:Potassium Channels / Q000235:genetics* / Q000502:physiology; D051381:Rats; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid
chemical_list: D009838:Oligodeoxyribonucleotides; D015221:Potassium Channels