Influence of steroid hormones on pS2/BCEI gene expression in xenografted colon tumors.
The biological function of the hormone inducible human pS2/BCEI gene, cloned from the breast cancer cell line MCF-7, still remains unknown. Our aim was to determine the in vivo influence of steroid hormones on pS2 expression and tumour growth in colorectal tumours. We transplanted aliquots of human colon tumours into male and female nude mice and studied tumour growth and expression of the pS2/BCEI gene by immunostaining and mRNA analysis. Our results show that the sex of the host and therefore the hormonal background does not play a dominant role in pS2 expression and growth of the xenografts.
Theisinger
B
B
Institute of Human Genetics, Univ. d. Saarlandes, Homburg, Germany.
Guthke
R
R
Blin
N
N
Welter
C
C
Seitz
G
G
D000818:Animals; D003110:Colonic Neoplasms; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D012739:Gonadal Steroid Hormones / Q000502:physiology*; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D009368:Neoplasm Transplantation; D011506:Proteins; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins
D012739:Gonadal Steroid Hormones; D009363:Neoplasm Proteins; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins