Chemoenzymic preparation of a glycoconjugate polymer having a sialyloligosaccharide: Neu5Ac alpha (2-->3)Gal beta (1-->4)GlcNAc.
Water-soluble polyacrylamide having 3'-sialyl N-acetyl-lactosamine [Neu5Ac alpha (2-->3)Gal beta (1-->4)GlcNAc] was enzymatically prepared by stepwise sugar-elongation on a water-soluble GlcNAc-bearing polyacrylamide. It was demonstrated that the flexible GlcNAc branches of the polymer chains allow quantitative galactosylation with bovine galactosyl transferase and partial sialylation by Trypanosoma cruzi trans-sialidase. Unsialylated N-acetyl-lactosamine side chains can be removed with beta-D-galactosidase and N-acetyl-beta-D-glucosaminidase to afford the targeted polymer containing 3'-sialyl N-acetyl-lactosamine.
Nishimura
S
S
Division of Biological Science, Graduate School of Science Hokkaido University, Sapporo, Japan.
Lee
K B
KB
Matsuoka
K
K
Lee
Y C
YC
D000116:Acetylgalactosamine / Q000737:chemistry; D002240:Carbohydrate Sequence; D006001:Glycoconjugates / Q000138:chemical synthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D012794:Sialic Acids / Q000737:chemistry
D006001:Glycoconjugates; D009844:Oligosaccharides; D012794:Sialic Acids; D000116:Acetylgalactosamine