Involvement of opioid peptides from casein on reticular motility and digesta passage in steers.
Experiment 1 determined the effects of incremental abomasal casein infusion on reticular motility and digesta passage of mature steers in a 4 x 4 Latin square study. Casein solutions were infused at 0, 1.25, 2.5, and 5.0% (wt/vol) for 3 h at 16.7 ml/min. Steers were fed for maintenance every 2 h. Casein resulted in a linear decrease in frequency, duration, and amplitude of reticular contractions measured manometrically. Total area of reticular contractions from physiographic tracings decreased 10.4 to 18.5%. Ruminal DM contents decreased 4.5 to 7.5%. Experiment 2 characterized the effects of casein on reticular contractions to be mediated by opioid peptides. Steers were infused abomasally (4 h) with either a 5% (wt/vol) solution of casein or its hydrolysate with or without a predose of naltrexone as an opiate antagonist (.5 mg/kg of BW) in a 2 x 2 factorial. Both casein and hydrolysate reduced frequency and duration of reticular contractions. Hydrolysate decreased contraction frequency within 30 min of infusion and 60 min prior to effects of casein. Naltrexone reversed the effects of casein and accentuated the effects of hydrolysate. Ruminal liquid outflow was decreased 19.7% with hydrolysate. Postruminal infusion of casein or its hydrolysate resulted in casomorphin-like activity on reticular motility and digesta passage.
Kil
S J
SJ
Department of Animal Science, University of Georgia, Athens 30602.
Froetschel
M A
MA
D000018:Abomasum / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002364:Caseins / Q000008:administration & dosage / Q000494:pharmacology*; D002417:Cattle / Q000502:physiology*; D004063:Digestion / Q000187:drug effects*; D004723:Endorphins / Q000502:physiology*; D005769:Gastrointestinal Motility / Q000187:drug effects*; D006868:Hydrolysis; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009271:Naltrexone / Q000494:pharmacology; D012159:Reticulum / Q000187:drug effects* / Q000502:physiology; D012996:Solutions
D002364:Caseins; D004723:Endorphins; D012996:Solutions; D009271:Naltrexone