Aspects of glucose uptake in Saccharomyces cerevisiae.
A wild-type Saccharomyces cerevisiae strain showed simple saturation kinetics for glucose uptake, with a Km of 4 mM when cells were obtained from exponential growth on glucose, and a similar, single Km of 2 to 8 mM was found under a variety of other growth conditions. Later in growth on glucose, and during ethanol utilization, a second kinetic component was observed, which might reflect either artifacts of membrane alteration or a Km in the molar range.
Gonçalves
T
T
Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, Portugal.
Loureiro-Dias
M C
MC
D001692:Biological Transport; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013045:Species Specificity
D000431:Ethanol; D005947:Glucose