title: [Follow-up of tissue acidosis, lactic acid production and morphologic changes in testicular tissue in ischemia and the effect of cooling].
abstract: More than 90% of the patients with a testicular torsion are loosing their testis by orchiectomy or by following ischemic atrophy. The critical time of irreversible changes of the testis is according to literature 4-6 hours. The aim of our study was to measure the tissue acidification (pH) in the testis at different temperatures and to estimate the cooling effect on pH, lactate accumulation and morphological changes. We have measured in 36 human testis (orchiectomy in patients with metastatic prostate cancer) the tissue acidification (pH), the tissue lactate level and the morphology changes at a temperature of 35, 25, 15 and 5 C. In 12 testis of young dogs we have measured the same parameters. Starting with a normal testis temperature of 35 C the pH is falling to 6.0 within 1 hour of ischemia. By colling to 15 C this time can be prolonged to 6 hours. The tissue lactate level rises from 25 mumol/gdw to nearly 200 mumol/gdw at 35 C. The morphology of semi-thick slices of the testis shows a swelling of the intratubular tissue (spermatogenesis) by loss of the interstitial space. By reducing temperature also a slowdown of the changes can be observed. The critical pH value of the testis for irreversible changes is not known. A ph of less than 6.0 is probably a value, which may result in such changes.
authors.affiliation: Urologische Klinik und Poliklinik, Georg August Universität, Göttingen.
authors.forename: M
authors.initials: M
authors.lastname: Kallerhoff
authors.forename: A
authors.initials: A
authors.lastname: Coors
authors.forename: C
authors.initials: C
authors.lastname: Fischer
authors.forename: H J
authors.initials: HJ
authors.lastname: Gröne
authors.forename: W
authors.initials: W
authors.lastname: Weidner
authors.forename: G
authors.initials: G
authors.lastname: Zöller
authors.forename: R H
authors.initials: RH
authors.lastname: Ringert
mesh_terms: D000136:Acid-Base Equilibrium / Q000502:physiology*; D000368:Aged; D000818:Animals; D001833:Body Temperature Regulation / Q000502:physiology*; D015925:Cryopreservation; D004285:Dogs; D006801:Humans; D007511:Ischemia / Q000473:pathology*; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D008297:Male; D008875:Middle Aged; D013737:Testis / Q000098:blood supply* / Q000473:pathology
chemical_list: D007773:Lactates; D019344:Lactic Acid
vernacular_title: Der Verlauf der Gewebeazidose, der Milchsäureproduktion und der morphologischen Veränderungen von Hodengewebe während der Ischämie und der Effekt von Kühlungsmassnahmen.