title: Evidence for the presence of "metabolic sterols" in Pneumocystis: identification and initial characterization of Pneumocystis carinii sterols.
abstract: Mixed life cycle stages of rat-derived Pneumocystis carinii were isolated from host lungs and their sterols were compared with those present in lungs from normal and immunosuppressed uninfected rats. Gas-liquid chromatography consistently detected, resolved, and quantified 9, 10, and 20 sterol components in the total nonsaponifiable neutral lipid fraction of lungs from normal rats, lungs from immunosuppressed uninfected rats, and P. carinii preparations, respectively. In all samples, cholesterol was the most abundant sterol present, comprising 97%, 93%, and 78% of total sterols in lungs from normal rats, lungs from immunosuppressed uninfected rats, and P. carinii, respectively. Tentative identifications of several rat lung and P. carinii minor sterols were made based on gas-liquid chromatogram retention times and fragmentation patterns from mass spectral analyses. Campesterol (ergost-5-en-3-ol), cholest-5-en-3-one, and beta-sitosterol (stigmast-5-en-3-ol) were among the minor components present in both types of lung controls, and were also components of P. carinii sterols. In contrast to lung controls, the sterols of P. carinii were enriched in C28 and C29 sterols with one or two double bonds, and a hydroxyl group at C-3 (ergost-5-en-3-ol, ergost-7-en-3-ol, ergosta-dien-3-ol, stigmast-5-en-3-ol, stigmast-7-en-3-ol and stigmasta-dien-3-ol). Steryl esters of P. carinii, probably stored in cytoplasmic lipid droplets, were dominated by those present in the host lung.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biological Sciences, University of Cincinnati, Ohio 45221.
authors.forename: E S
authors.initials: ES
authors.lastname: Kaneshiro
authors.forename: J E
authors.initials: JE
authors.lastname: Ellis
authors.forename: K
authors.initials: K
authors.lastname: Jayasimhulu
authors.forename: D H
authors.initials: DH
authors.lastname: Beach
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D016867:Immunocompromised Host; D008168:Lung / Q000469:parasitology; D013058:Mass Spectrometry; D011010:Pneumocystis / Q000378:metabolism*; D011020:Pneumonia, Pneumocystis / Q000276:immunology / Q000469:parasitology; D051381:Rats; D013261:Sterols / Q000378:metabolism*
chemical_list: D013261:Sterols