A comparison of the reduction in immunoglobulin (IgG) concentration of frozen equine plasma treated by three thawing techniques.
The IgG concentration of plasma from 13 mares was measured by radial immunodiffusion when fresh and after storage at -4 degrees C and thawing by 3 methods. There was no significant (P > 0.05) reduction in the IgG concentration when plasma was thawed over 6 hours at 22 degrees C (1352.9 +/- 101.6 mg/dL) (mean +/- SEM) compared with the fresh sample (1369.5 +/- 88.1 mg/dL). In contrast, there was a significant (P < 0.05) reduction in the IgG concentration of plasma that was rapidly thawed at 57 degrees C over 50 minutes (1142.9 +/- 66.2 mg/dL), or placed in a microwave oven for 20 to 25 minutes (1093.4 +/- 74.4 mg/dL). When plasma is administered to hypo-gammaglobulinaemic newborn foals, a larger volume is required when thawing is rapid than when thawing is slow or when fresh plasma is administered.
O'Rielly
J L
JL
Department of Veterinary Clinic and Hospital, University of Melbourne, Werribee, Victoria.
D000818:Animals; D005260:Female; D006736:Horses / Q000097:blood* / Q000276:immunology; D006358:Hot Temperature; D007074:Immunoglobulin G / Q000097:blood* / Q000528:radiation effects; D007185:Incubators / Q000662:veterinary; D008872:Microwaves; D010949:Plasma / Q000276:immunology* / Q000528:radiation effects
D007074:Immunoglobulin G