title: Effect of portal hypertension on bacterial translocation induced by major liver resection in rats.
abstract: OBJECTIVE
  To evaluate the influence of portal hypertension on bacterial translocation from the gut caused by major hepatic resection.
  
  
  METHODS
  Randomised study.
  
  
  METHODS
  University of Lund, Sweden.
  
  
  METHODS
  228 adult male Sprague-Dawley rats.
  
  
  METHODS
  Laparotomy and separation of the liver from its attachments (sham operated group), 70% hepatic resection, 90% hepatic resection, or subtotal ligation of the portal vein. For assessment of the degree of bacterial translocation a further laparotomy was done, 0.2 ml of blood taken from the portal vein and the vena cava, and specimens taken of mesenteric lymph nodes, liver, spleen, lungs, proximal jejunum, distal ileum, and transverse colon. Microcirculation of the intestine was measured by laser Doppler flowmetry with the probe held gently against the bowel wall at six points randomly chosen from the distal small intestine and colon.
  
  
  METHODS
  Alterations in portal venous pressure, arterial pressure, and bacterial translocation to other organs, particularly mesenteric lymph nodes.
  
  
  RESULTS
  Bacterial translocation increased significantly after 90% hepatectomy, and there was an increase in portal pressure together with a decrease in systemic arterial pressure after both 90% hepatectomy and subtotal ligation of the portal vein. Subserosal blood flow to the distal small intestine decreased after 70% resection, 90% resection, and subtotal ligation of the portal vein; subserosal blood flow to the colon decreased only after 90% hepatectomy.
  
  
  CONCLUSIONS
  Increased portal venous pressure after major hepatic resection is not the sole cause of the increase in the incidence of bacterial translocation from the gut in rats.
authors.affiliation: Department of Surgery, Lund University Hospital, Sweden.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Andersson
authors.forename: V
authors.initials: V
authors.lastname: Soltesz
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Bengmark
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000453:epidemiology / Q000382:microbiology*; D018407:Bacterial Physiological Phenomena; D002465:Cell Movement; D006498:Hepatectomy; D006975:Hypertension, Portal / Q000503:physiopathology / Q000601:surgery; D015994:Incidence; D007422:Intestines / Q000382:microbiology*; D008026:Ligation; D008198:Lymph Nodes / Q000382:microbiology*; D008297:Male; D008643:Mesentery; D011169:Portal Vein / Q000382:microbiology* / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014690:Venous Pressure