title: DNA content analysis of Barrett's esophagus by flow cytometry.
abstract: The purpose of this study was to investigate the correlation of flow cytometric (FCM) DNA content and cell cycle characteristics of Barrett's Esophagus (BE) with age and sex of the patients, length, histologic type and dysplasia of BE. Forty-one patients affected by histologically confirmed BE had multiple biopsies taken from the metaplastic epithelium and one biopsy taken from the gastric fundus, as control. The samples were either stored at -80 degrees C or immediately measured. Nuclei suspensions were obtained, stained with DAPI, measured with a high resolution flow cytometer (ICP22A, Ortho Instruments) and analyzed for the evaluation of the relative DNA content and the S- and G2 + M phases of the cell cycle. DNA histograms having two distinct G0/G1 peaks were classified as DNA aneuploid. The degree of DNA aneuploidy (DNA Index, DI), defined as the ratio of abnormal to normal DNA content, was obtained from the mixture of each BE sample with its control sample and trout erythrocytes. We found six patients with DNA aneuploid populations (14.6%), whose DNA Index values were 1.05 (in two), 1.8 (in one), and 2.0 (in three cases). DNA ploidy did not correlate with age and sex of the patients, length, histologic type, and dysplasia of BE. Among the 13 patients with dysplasia (6 indefinite, 4 low grade and 2 high grade with intramucous adenocarcinoma) only two (one indefinite and one low grade) showed DNA aneuploidy (15.4%). In addition, we found that the S-phase and the G2 + M-phase fractions in BE samples were both significantly higher than those of the controls (respectively, p = 0.01 and p = 0.0008).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Servizio di Epidemiologia Clinica, Università di Brescia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Sciallero
authors.forename: W
authors.initials: W
authors.lastname: Giaretti
authors.forename: L
authors.initials: L
authors.lastname: Bonelli
authors.forename: E
authors.initials: E
authors.lastname: Geido
authors.forename: A
authors.initials: A
authors.lastname: Rapallo
authors.forename: M
authors.initials: M
authors.lastname: Conio
authors.forename: P
authors.initials: P
authors.lastname: Ravelli
authors.forename: L
authors.initials: L
authors.lastname: Lombardo
authors.forename: R
authors.initials: R
authors.lastname: Briglia
authors.forename: G
authors.initials: G
authors.lastname: Lapertosa
mesh_terms: D001471:Barrett Esophagus / Q000235:genetics* / Q000473:pathology; D001706:Biopsy; D004247:DNA / Q000235:genetics*; D004947:Esophagus / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D016196:S Phase
chemical_list: D004247:DNA