title: Monensin and forskolin inhibit the transcription rate of sucrase-isomaltase but not the stability of its mRNA in Caco-2 cells.
abstract: Treatment of Caco-2 cells with forskolin (25 microM) or monensin (1 microM) has previously been shown to cause a marked decrease in the level of sucrase-isomaltase (SI) mRNA, without any effect on the expression of dipeptidylpeptidase IV (DPP-IV). In the present work, we report that there is no significant difference in the stability of SI mRNA between control and treated cells. On the other hand, we demonstrate a decrease in the transcription rate of SI mRNA which is sufficient to account for the decrease in the steady-state level of SI mRNA both in forskolin- and monensin-treated Caco-2 cells.
authors.affiliation: Unité de Recherches sur la Différenciation Cellulaire Intestinale, INSERM U178, Villejuif, France.
authors.forename: I
authors.initials: I
authors.lastname: Chantret
authors.forename: M
authors.initials: M
authors.lastname: Lacasa
authors.forename: G
authors.initials: G
authors.lastname: Chevalier
authors.forename: D
authors.initials: D
authors.lastname: Swallow
authors.forename: M
authors.initials: M
authors.lastname: Rousset
mesh_terms: D015152:Blotting, Northern; D005576:Colforsin / Q000494:pharmacology*; D015342:DNA Probes; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D008985:Monensin / Q000494:pharmacology*; D009834:Oligo-1,6-Glucosidase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013393:Sucrase / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D005576:Colforsin; D008985:Monensin; D009834:Oligo-1,6-Glucosidase; D013393:Sucrase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4