Erythrocyte cation transport systems and membrane lipids in insulin-dependent diabetes.
The relationship between erythrocyte cation transport systems and membrane and plasma lipids was examined in normal men and patients with insulin-dependent diabetes mellitus (IDDM). Different measurements of erythrocyte transport systems were obtained in patients with IDDM and in age- and weight-matched healthy men: Na+:Li+ countertransport activity or Li(+)-stimulated Na+ efflux, Na+:K+ cotransport activity or bumetanide-sensitive Na+ efflux, Na+:K+ pump activity or ouabain-sensitive Na+ efflux, and the ouabain- and bumetanide-resistant Na+ and K+ fluxes or the ground membrane permeability for Na+ and K+ as well as the intraerythrocyte Na+, K+, and Mg2+ concentrations. Plasma cholesterol, triglycerides, phospholipids, low- and high- density lipoprotein cholesterol, and erythrocyte membrane cholesterol and phospholipid content were obtained from the fasting subjects. The patients with IDDM had an elevated (P < .05 or less) erythrocyte Na+:Li+ countertransport activity, ground membrane leak for K+, intraerythrocyte K+ concentration, and erythrocyte membrane cholesterol content, but a lower red blood cell phospholipid content. In single regression analysis, the erythrocyte Na+:Li+ countertransport, Na+:K+ cotransport, and Na+:K+ pump activity and ground membrane leak for Na+ and K+ were inversely related to the red blood cell membrane lipid content. Our data in patients with IDDM show that a decreased erythrocyte membrane lipid content was accompanied by a higher erythrocyte Na+:Li+ countertransport, Na+:K+ cotransport, and Na+:K+ pump activity.
Lijnen
P
P
Hypertension and Cardiovascular Rehabilitation Unit, University of Leuven, Belgium.
Fenyvesi
A
A
Bex
M
M
Bouillon
R
R
Amery
A
A
D000328:Adult; D002412:Cations / Q000097:blood*; D002463:Cell Membrane Permeability / Q000502:physiology; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D008094:Lithium / Q000097:blood; D008274:Magnesium / Q000097:blood; D008297:Male; D008563:Membrane Lipids / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D012044:Regression Analysis; D012964:Sodium / Q000097:blood; D014280:Triglycerides / Q000097:blood
D002412:Cations; D008076:Cholesterol, HDL; D008563:Membrane Lipids; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol; D008094:Lithium; D012964:Sodium; D008274:Magnesium