title: Adenosine A1 receptor-mediated inhibition of evoked glutamate release is coupled to calcium influx decrease in goldfish brain synaptosomes.
abstract: Binding of [3H]cyclohexyladenosine (CHA) to the cellular fractions and P2 subfractions of the goldfish brain was studied. The A1 receptor density was predominantly in synaptosomal membranes. In goldfish brain synaptosomes (P2), 30 mM K+ stimulated glutamate, taurine and GABA release in a Ca(2+)-dependent fashion, whereas the aspartate release was Ca(2+)-independent. Adenosine, R-phenylisopropyladenosine (R-PIA) and CHA (100 microM) inhibited K(+)-stimulated glutamate release (31%, 34% and 45%, respectively). All of these effects were reversed by the selective adenosine A1 receptor antagonist, 8-cyclopentyltheophylline (CPT). In the same synaptosomal preparation, K+ (30 mM) stimulated Ca2+ influx (46.8 +/- 6.8%) and this increase was completely abolished by pretreatment with 100 nM omega-conotoxin. Pretreatment with 100 microM R-PIA or 100 microM CHA, reduced the evoked increase of intra-synaptosomal Ca2+ concentration, respectively by 37.7 +/- 4.3% and 39.7 +/- 9.0%. A possible correlation between presynaptic A1 receptor inhibition of glutamate release and inhibition of calcium influx is discussed.
authors.affiliation: Department of Biology, Faculty of Sciences, University of Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Poli
authors.forename: R
authors.initials: R
authors.lastname: Lucchi
authors.forename: M
authors.initials: M
authors.lastname: Zottini
authors.forename: U
authors.initials: U
authors.lastname: Traversa
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D016257:Fura-2; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006054:Goldfish; D007700:Kinetics; D010455:Peptides / Q000494:pharmacology; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011983:Receptors, Purinergic / Q000187:drug effects / Q000502:physiology*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology; D020960:omega-Conotoxins
chemical_list: D000596:Amino Acids; D002121:Calcium Channel Blockers; D005971:Glutamates; D010455:Peptides; D011983:Receptors, Purinergic; D020960:omega-Conotoxins; C053423:Conus magus toxin; D010660:Phenylisopropyladenosine; C053907:8-cyclopentyl-1,3-dimethylxanthine; C027513:N(6)-cyclohexyladenosine; D018698:Glutamic Acid; D013806:Theophylline; D000241:Adenosine; D011188:Potassium; D002118:Calcium; D016257:Fura-2