Usefulness of a new perchloric acid treatment method to measure endotoxin in cerebrospinal fluid by the limulus test.
The modernized limulus lysate assay on cerebrospinal fluid pretreated by the new perchloric acid (PCA) treatment was evaluated in one patient with Escherichia coli meningitis and four non-meningitis patients. The endotoxin (ET) concentration was much higher by the new PCA treatment than by the ordinary dilution method in the exacerbation phase of E. coli meningitis. The recovery rate of added ET also demonstrated the superiority of the new PCA treatment.
Suda
H
H
Perinatal Medical Center, Morioka Red Cross Hospital, Japan.
Inada
K
K
Yoshida
M
M
D003124:Colorimetry; D004731:Endotoxins / Q000134:cerebrospinal fluid*; D004926:Escherichia coli; D005260:Female; D006801:Humans; D007223:Infant; D008033:Limulus Test / Q000379:methods*; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D010472:Perchlorates
D004731:Endotoxins; D010472:Perchlorates