Interleukin-1 alpha, interleukin-1 beta, and tumor necrosis factor gene expression in endotoxin-induced uveitis.
OBJECTIVE
To localize and determine the levels of interleukin-1 alpha (IL-1 alpha), interleukin-1 beta (IL-1 beta), and tumor necrosis factor (TNF) gene expression in the process of endotoxin-induced uveitis (EIU) in rats.


METHODS
EIU was induced by lipopolysaccharide (LPS) injection in male Lewis rats weighing 150 to 200 g. The levels of IL-1 alpha, IL-1 beta, and TNF gene expression in the iris-ciliary body (ICB) were quantified by a semiquantitative polymerase chain reaction (PCR) method; in situ hybridization histochemistry was carried out to localize the gene transcripts.


RESULTS
Little expression of IL-1 alpha, IL-1 beta, and TNF genes was observed in normal ICB. IL-1 alpha gene expression began to increase (about 10-fold greater than that of the control) as early as 1 hour after the LPS treatment, reached a peak (about 100-fold) at 3 to 6 hours. A second peak (60-fold) was observed at 24 hours, and the expression returned to near basal levels (3-fold) at 48 hours. Expression of IL-1 beta and TNF genes showed a pattern similar to that of IL-1 alpha. Three hours after LPS treatment, IL-1 beta and TNF genes were found by in situ hybridization histochemistry to be expressed by "histiocyte-like" cells in the stroma of the ICB. None of these genes were detected in the control rats.


CONCLUSIONS
Expression of IL-1 alpha, IL-1 beta, and TNF genes was dramatically up-regulated in the process of EIU. These genes were found to be expressed in "histiocyte-like" cells in the ICB, and may have an important role in EIU.
Yoshida
M
M
Department of Ophthalmology, Kyoto University Faculty of Medicine, Japan.
Yoshimura
N
N
Hangai
M
M
Tanihara
H
H
Honda
Y
Y
D000818:Animals; D001427:Bacterial Toxins; D001483:Base Sequence; D002924:Ciliary Body / Q000378:metabolism; D017931:DNA Primers; D004731:Endotoxins; D004768:Enterotoxins; D015870:Gene Expression; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D007498:Iris / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D011917:Rats, Inbred Lew; D012475:Salmonella; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D014606:Uveitis, Anterior / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology
D001427:Bacterial Toxins; D017931:DNA Primers; D004731:Endotoxins; D004768:Enterotoxins; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; C027952:salmonella toxin