Registration of myometrial activity using multiple site electromyography in cyclic mares.
A method for interpreting and analysing electromyographic (EMG) data of myometrial electrical activity was established. This method was used to study EMG activity in the uterus during the various stages of the oestrus cycle in mares. Recordings were analysed from four pairs of electrodes that were surgically implanted in the myometrium of four reproductively sound mares. The electrodes were placed at the tip, middle and base of the left horn and in the uterine body. Electrical activity was monitored by a polygraph. Data were transformed to a digitized form and statistically analysed. Myometrial electrical activity during oestrus and dioestrus was analysed for frequency (number of activity bursts per hour), duration (time elapsed from the start to the end of a burst) and intensity (peaks per minute). In addition, the degrees of synchronous activity among all of the monitored sites of the uterus were compared. The minimal amount of time of EMG recordings that allowed meaningful statistical analyses was determined to be 3.5 h during oestrus and 7 h during dioestrus. Variations in intensity of electrical activity were seen between the sites of implanted electrodes (P < 0.001). Electrical activity was highest in the middle of the horn and lowest in the uterine body. The frequency of activity bursts was the same during oestrus and dioestrus (4.35 +/- 0.22 and 4.44 +/- 0.20, respectively). The duration and intensity of uterine electrical activity depended on the stage of the oestrous cycle. The mean duration of uterine activity burst during dioestrus was significantly (P < 0.005) shorter during oestrus (3.32 +/- 0.18 min) than during dioestrus (5.7 +/- 0.39 min).(ABSTRACT TRUNCATED AT 250 WORDS)
Troedsson
M H
MH
Department of Reproduction, School of Veterinary Medicine, University of California, Davis 95616.
Wiström
A O
AO
Liu
I K
IK
Ing
M
M
Pascoe
J
J
Thurmond
M
M
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D004031:Diestrus / Q000502:physiology; D004576:Electromyography; D004971:Estrus / Q000502:physiology*; D005260:Female; D006736:Horses / Q000097:blood / Q000502:physiology*; D009215:Myometrium / Q000502:physiology*; D011374:Progesterone / Q000097:blood
D011374:Progesterone