title: Detection of monoclonal hematopoiesis using restriction fragment length polymorphism (RFLP) of phosphoglycerokinase (PGK) gene in a patient with hypoplastic myelodysplastic syndrome (MDS).
abstract: A clonal analysis of hematopoiesis in a hypoplastic MDS patient was performed. Monoclonal utilization of PGK gene on her two X chromosomes was detected by Southern blot analysis of the bone marrow cells using a PGK probe after methylation-sensitive restriction enzyme treatment. This is compatible with monoclonal construction of her hematopoiesis, confirming MDS nature with a progression of her morphological dysplasia in bone marrow cells. RFLP of PGK gene on X chromosome is thus useful to detect a monoclonality in hematopoietic diseases.
authors.affiliation: Department of Internal Medicine, Ohmura Municipal Hospital, Nagasaki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: K
authors.initials: K
authors.lastname: Kuriyama
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.forename: I
authors.initials: I
authors.lastname: Jinnai
authors.forename: K
authors.initials: K
authors.lastname: Hirashima
authors.forename: M
authors.initials: M
authors.lastname: Tomonaga
mesh_terms: D000293:Adolescent; D001853:Bone Marrow / Q000473:pathology; D005260:Female; D006410:Hematopoiesis / Q000235:genetics*; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000503:physiopathology*; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D010735:Phosphoglycerate Kinase