Low-molecular-mass human salivary mucin, MG2: structure and binding of Pseudomonas aeruginosa.
Low-molecular-mass human salivary mucin, MG2, was isolated from human submandibular-sublingual saliva (HSMSL) employing citraconylation, gel filtration, and ion-exchange chromatography. Following proteolysis with trypsin, two glycopeptides were purified. The higher molecular weight glycopeptide was highly glycosylated with O-linked units. The lower molecular weight glycopeptide was less glycosylated and contained most of the N-linked units. Interaction between components of HSMSL and pili of Pseudomonas aeruginosa was examined by an overlay binding assay. Pili were found to bind to MG2. Preliminary studies indicated that the binding may involve a protein to protein interaction.
Reddy
M S
MS
Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo 14214.
Levine
M J
MJ
Paranchych
W
W
D000409:Alanine / Q000032:analysis; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000378:metabolism; D006020:Glycopeptides / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D007532:Isoleucine / Q000032:analysis; D007930:Leucine / Q000032:analysis; D008969:Molecular Sequence Data; D009077:Mucins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D009844:Oligosaccharides / Q000032:analysis; D010649:Phenylalanine / Q000032:analysis; D011392:Proline / Q000032:analysis; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D014357:Trypsin / Q000032:analysis / Q000302:isolation & purification; D014633:Valine / Q000032:analysis
D001426:Bacterial Proteins; D006020:Glycopeptides; C083238:MUC7 protein, human; D009077:Mucins; D009844:Oligosaccharides; D012471:Salivary Proteins and Peptides; D007532:Isoleucine; D010649:Phenylalanine; D011392:Proline; D014357:Trypsin; D007930:Leucine; D014633:Valine; D000409:Alanine