Human hepatoma cells expressing HLA class I molecules stimulate primary responses of purified CD8+ T lymphocytes.
In the present study, we investigated the ability of major histocompatibility complex (MHC) class-I+ human hepatoma cell lines to induce primary proliferative responses of purified allogeneic CD8+ T lymphocytes. We found that HA22T/VGH and Li7A, but not HepG2 cells induced significant proliferation of CD8+ T cells and that these responses were dependent on class I molecule expression. In blocking experiments carried out to identify the costimulatory signals involved, we found that anti-ICAM1 monoclonal antibodies drastically inhibited CD8+ T-cell proliferative responses. These findings suggest that transformed hepatocytes expressing HLA class I molecules may participate in anti-tumour immunosurveillance by the direct induction of cytotoxic T-cell responses through ICAM1-mediated adhesive interaction.
Paroli
M
M
Fondazione Andrea Cesalpino, I Clinica Medica, Università La Sapienza, Roma, Italy.
Carloni
G
G
Franco
A
A
De Petrillo
G
G
Alfani
E
E
Barnaba
V
V
D000911:Antibodies, Monoclonal; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000032:analysis; D005434:Flow Cytometry; D005805:Genes, MHC Class I / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007376:Interleukin-2 / Q000235:genetics; D007519:Isoantigens / Q000276:immunology; D008113:Liver Neoplasms / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D012333:RNA, Messenger / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016827:CD8 Antigens; D015815:Cell Adhesion Molecules; D007376:Interleukin-2; D007519:Isoantigens; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1