Identification of Evi-3, a novel common site of retroviral integration in mouse AKXD B-cell lymphomas.
We have identified a novel common site of ecotropic viral integration called ecotropic viral integration site 3 (Evi-3) in B-cell lineage lymphomas of the AKXD recombinant inbred strains of mice. A number of virally induced pre-B-, B-, myeloid, and T-cell lymphomas were screened for viral rearrangements at Evi-3; rearrangements were found in pre-B- and B-cell lymphomas but not in other hematopoietic tumors. Genetic mapping studies localized Evi-3 to mouse chromosome 18, distinct from proto-oncogene and common viral integration loci identified previously in the mouse. Each proviral integration at Evi-3 is contained within a 200-bp region that lies inside a CpG island. All but one of the proviruses have integrated in the same 5'-to-3' transcriptional orientation. Transcripts from Evi-3 are expressed in a developmentally regulated manner in B cells. Taken together, these data suggest that Evi-3 represents a novel proto-oncogene involved in mouse B-cell lymphomas.
Justice
M J
MJ
Division of Biology, Kansas State University, Manhattan 66506.
Morse
H C
HC
Jenkins
N A
NA
Copeland
N G
NG
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004273:DNA, Neoplasm / Q000235:genetics*; D015226:Dinucleoside Phosphates / Q000235:genetics; D015321:Gene Rearrangement; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000382:microbiology; D051379:Mice; D008815:Mice, Inbred Strains; D011519:Proto-Oncogenes / Q000235:genetics; D011533:Proviruses / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics*; D016662:Virus Integration / Q000235:genetics*
D004273:DNA, Neoplasm; D015226:Dinucleoside Phosphates; D012333:RNA, Messenger; C015772:CpG dinucleotide