Incorporation of radioactive phosphate from adenosine triphosphate into phosphatidylinositol-4-phosphate in human peripheral blood hypodense eosinophils is less than in the normodense eosinophils.
Peripheral blood eosinophils from patients with atopic dermatitis and normal healthy controls were isolated on a Percoll gradient and incubated with [gamma 32P]ATP in the presence of Mg2+. After stopping the reaction, lipid extraction was performed with acidic medium and phospholipids were separated by thin-layer chromatography on 1% (w/v) oxalic acid-impregnated silica gel plates. In the autoradiographs considerable amounts of the radioactivity were found to be incorporated into phosphatidylinositol-4-phosphate. 32P incorporation into phosphatidylinositol-4-phosphate in hypodense eosinophils from atopic dermatitis patients was less than in normodense eosinophils from the same patients and controls.
Kurosawa
M
M
First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Inamura
H
H
Shimizu
Y
Y
D000255:Adenosine Triphosphate / Q000737:chemistry*; D000293:Adolescent; D000328:Adult; D001345:Autoradiography; D002452:Cell Count; D002855:Chromatography, Thin Layer; D003876:Dermatitis, Atopic / Q000097:blood*; D004804:Eosinophils / Q000737:chemistry* / Q000166:cytology; D005260:Female; D006801:Humans; D008297:Male; D010710:Phosphates / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000097:blood*; D010761:Phosphorus Radioisotopes
D010710:Phosphates; D018129:Phosphatidylinositol Phosphates; D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate