Resolution and sensitivity enhancement of heteronuclear correlation for methylene resonances via 2H enrichment and decoupling.
Monodeuterated methylene positions exhibit substantially superior spectral characteristics in 1H-13C correlation experiments as compared to diprotio signals. A combination of 2H decoupling and multiplet editing of HMQC and HSQC experiments provides resolution enhancement for both stereoselective and random fractionally deuterated samples. For HMQC experiments with [2-2HR,2-13C]glycine-labeled E. coli thioredoxin (11.7 kDa), 3-fold increases in both 1H and 13C resolution result in a complementary 9-fold enhancement in sensitivity. Owing to a smaller improvement in 13C resolution, the corresponding enhancements for the HSQC experiment are 2-fold less.
Kushlan
D M
DM
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208.
LeMaster
D M
DM
D002247:Carbon Isotopes; D004926:Escherichia coli / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D006838:Hydrocarbons; D006859:Hydrogen / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D011522:Protons; D012680:Sensitivity and Specificity; D013879:Thioredoxins / Q000737:chemistry
D002247:Carbon Isotopes; D005609:Free Radicals; D006838:Hydrocarbons; D011522:Protons; C030011:carbene; D013879:Thioredoxins; D006859:Hydrogen; D008697:Methane