Quantitation of paraquat in serum by HPLC.
An HPLC method for the quantitation of paraquat in urine was applied to serum. Sample preparation consisted of ion-pair extraction on disposable cartridges of end-capped octadecyl silica. The paraquat thus extracted, was quantitated by HPLC using 1,1'-diethyl-4,4'-dipyridyl dichloride as an internal standard. Relatively small sample volumes (1 mL serum) were required. Within- and between-assay coefficients of variation for the HPLC technique were 2.9% and 4.0%, respectively, at low paraquat serum concentration (0.4 microgram/mL), and 2.4% and 3.2% at high paraquat serum concentration (4.0 micrograms/mL). The between-assay coefficient of variation for the total procedure, including the extraction, was 5.6% at low concentration (0.4 microgram/mL). Serum concentration levels down to 0.025 microgram/mL could be quantitated. The technique was checked for interference from muscle relaxants (pancuronium bromide and vecuronium bromide) and from anticoagulants (heparin and K2EDTA). No interference was observed.
Croes
K
K
Laboratory of Emergency Toxicology, U.Z. Gasthuisberg, Leuven, Belgium.
Martens
F
F
Desmet
K
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D010269:Paraquat / Q000097:blood* / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D010269:Paraquat