A method for simultaneous detection of fluorescent G-bands and in situ hybridization signals.
Improved techniques for detecting in situ hybridization signals on fluorescent G-bands are described. Prior to hybridization of biotinylated DNA probes, chromosomes with replication G-bands were treated with formaldehyde and denatured with an alkaline solution. These steps preserve chromosome morphology well, and enable us to simultaneously demonstrate fluorescein hybridization signals and G-banded chromosomes stained with propidium iodide. This method is useful for precise chromosomal localization of DNA markers.
Hirai
M
M
Department of Anthropology, Graduate School of Science, University of Tokyo, Japan.
Suto
Y
Y
Kanoh
M
M
D002871:Chromosome Banding / Q000379:methods*; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008285:Major Histocompatibility Complex / Q000235:genetics
D015342:DNA Probes