Direct association of adenosine deaminase with a T cell activation antigen, CD26.
CD26, the T cell activation molecule dipeptidyl peptidase IV (DPPIV), associates with a 43-kilodalton protein. Amino acid sequence analysis and immunoprecipitation studies demonstrated that this 43-kilodalton protein was adenosine deaminase (ADA). ADA was coexpressed with CD26 on the Jurkat T cell lines, and an in vitro binding assay showed that the binding was through the extracellular domain of CD26. ADA deficiency causes severe combined immunodeficiency disease (SCID) in humans. Thus, ADA and CD26 (DPPIV) interact on the T cell surface, and this interaction may provide a clue to the pathophysiology of SCID caused by ADA deficiency.
Kameoka
J
J
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA.
Tanaka
T
T
Nojima
Y
Y
Schlossman
S F
SF
Morimoto
C
C
D000243:Adenosine Deaminase / Q000032:analysis / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000502:physiology*; D002352:Carrier Proteins / Q000032:analysis; D002460:Cell Line; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000032:analysis; D006801:Humans; D008213:Lymphocyte Activation / Q000502:physiology; D011485:Protein Binding; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D002352:Carrier Proteins; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4; D000243:Adenosine Deaminase