Detection of monoclonal hematopoiesis using restriction fragment length polymorphism (RFLP) of phosphoglycerokinase (PGK) gene in a patient with hypoplastic myelodysplastic syndrome (MDS).
A clonal analysis of hematopoiesis in a hypoplastic MDS patient was performed. Monoclonal utilization of PGK gene on her two X chromosomes was detected by Southern blot analysis of the bone marrow cells using a PGK probe after methylation-sensitive restriction enzyme treatment. This is compatible with monoclonal construction of her hematopoiesis, confirming MDS nature with a progression of her morphological dysplasia in bone marrow cells. RFLP of PGK gene on X chromosome is thus useful to detect a monoclonality in hematopoietic diseases.
Miyazaki
Y
Y
Department of Internal Medicine, Ohmura Municipal Hospital, Nagasaki, Japan.
Kuriyama
K
K
Fujiwara
T
T
Jinnai
I
I
Hirashima
K
K
Tomonaga
M
M
D000293:Adolescent; D001853:Bone Marrow / Q000473:pathology; D005260:Female; D006410:Hematopoiesis / Q000235:genetics*; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000503:physiopathology*; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D010735:Phosphoglycerate Kinase