Inhibition of murine AIDS (MAIDS), development by the transplantation of bone marrow cells carrying the Fv-4 resistance gene to MAIDS virus-infected mice.
To examine whether the resistance allele of the Fv-4 gene (the Fv-4r gene) is a dominant inhibitory-product-encoding gene which an be used to prevent the development of murine AIDS (MAIDS), bone marrow cells from BALB/c-Fv-4wr mice were transplanted into BALB/c mice and C57BL/6 mice infected with MAIDS virus. Almost all of the virus-infected BALB/c and C57BL/6 mice developed MAIDS within 4 months and died 2 or 3 months later. However, when the virus-infected mice were subjected to cobalt irradiation and then given an intravenous injection of 10(7) BALB/c-Fv-4wr mouse bone marrow cells, the recipient mice survived much longer than the untreated mice, which suggests that the Fv-4 gene is a dominant inhibitory gene that is potentially useful in gene therapy of MAIDS.
Nakagawa
Y
Y
Institute of Virus Research, Kyoto University, Japan.
Kakimi
K
K
Ling
W
W
Kubo
Y
Y
Higo
K
K
Masuda
T
T
Kuribayashi
K
K
Iwashiro
M
M
Komatz
Y
Y
Hirama
T
T
D000818:Animals; D000831:Animals, Newborn; D016026:Bone Marrow Transplantation; D005434:Flow Cytometry; D005799:Genes, Dominant / Q000235:genetics*; D015316:Genetic Therapy / Q000379:methods*; D006183:H-2 Antigens / Q000032:analysis; D007113:Immunity, Innate / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000401:mortality / Q000628:therapy*; D013154:Spleen / Q000276:immunology; D016019:Survival Analysis
D006183:H-2 Antigens