Immunohistochemical studies of S-100 protein expression in myoepithelial cells of benign breast diseases and normal breast tissues.
We studied benign breast diseases using polyclonal anti S-100 protein and monoclonal anti a-subunit or beta-subunit of S-100 protein antibodies. The antibody gave positive staining in most of the cytoplasms and nuclei of the myoepithelial cells of normal breast tissues, gynecomastia, fibroadenoma, intraductal papilloma and mastopathy. However, immunohistochemical methods using the monoclonal antibodies for them did not reveal positive staining in the myoepithelial cells.
Umekita
Y
Y
Department of Pathology, Faculty of Medicine, Kagoshima University, Japan.
Fujiyoshi
T
T
Takasaki
T
T
Kuriwaki
K
K
Yoshida
A
A
Yoshida
H
H
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001940:Breast / Q000378:metabolism*; D001941:Breast Diseases / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry; D004848:Epithelium / Q000378:metabolism; D018226:Fibroadenoma / Q000378:metabolism; D005348:Fibrocystic Breast Disease / Q000378:metabolism; D006177:Gynecomastia / Q000378:metabolism; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis; D009130:Muscle, Smooth / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis; D018300:Papilloma, Intraductal / Q000378:metabolism; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000906:Antibodies; D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D009124:Muscle Proteins; D009363:Neoplasm Proteins; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C077591:S100A4 protein, human