title: Antigenotoxic properties of lactic acid bacteria in vivo in the gastrointestinal tract of rats.
abstract: In view of the high incidence of dietary-related tumors, one important research goal is to identify the participating genotoxic carcinogens and the nutritional factors that may counteract their activities. We therefore have further developed a method to assess DNA damage in tumor target tissues of the gastrointestinal tract. Subsequently the prevention of this inducible DNA damage by lactic acid bacteria and by milk products fermented with probiotics was studied as well. The microgel electrophoresis technique was applied to cells of the esophageal, gastric, duodenal, and colonic mucosa. Cells were grouped according to their degree of DNA damage, the simplest measure of which is to discriminate between those with damage (comets) and those without damage. When these cells were isolated from animals treated with a genotoxic carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and exposed to MNNG for 1-24 hours, it was possible to follow the course of genotoxicity throughout the gastrointestinal tract. After the animals were treated with the lactic acid bacteria under study, it was possible to detect antigenotoxic properties as well. The gavage of 10(10) viable Lactobacillus casei cells in 10 ml of 0.9% NaCl per kilogram body weight immediately before the oral administration of MNNG (5 mg/kg body wt) resulted in a reduction of induced DNA damage in gastric and colonic mucosa cells. A sequential treatment schedule was even more effective: when the animals were treated orally with lactic acid bacteria or yogurt (10 ml/kg body wt) in the morning followed by MNNG (7.5 mg/kg body wt) eight hours later and the colon cells were isolated 16 hours later, the percentages of cells remaining intact were distinctly higher in the combination groups (68 +/- 10 and 68 +/- 19 for L. casei and a "Bio" yogurt, respectively) than in the group receiving only MNNG (45 +/- 17). The effect of heating L. casei was studied and was found to yield less clear-cut effects in preventing genotoxicity. The method is an efficient tool to elucidate antigenotoxic properties of food components in vivo in those target tissues actually afflicted by dietary-related tumors.
authors.affiliation: Institute of Hygiene and Toxicology, Federal Research Center for Nutrition, FRG.
authors.forename: B L
authors.initials: BL
authors.lastname: Pool-Zobel
authors.forename: B
authors.initials: B
authors.lastname: Bertram
authors.forename: M
authors.initials: M
authors.lastname: Knoll
authors.forename: R
authors.initials: R
authors.lastname: Lambertz
authors.forename: C
authors.initials: C
authors.lastname: Neudecker
authors.forename: U
authors.initials: U
authors.lastname: Schillinger
authors.forename: P
authors.initials: P
authors.lastname: Schmezer
authors.forename: W H
authors.initials: WH
authors.lastname: Holzapfel
mesh_terms: D000818:Animals; D004249:DNA Damage; D005285:Fermentation; D005770:Gastrointestinal Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D007778:Lactobacillus / Q000502:physiology*; D008297:Male; D008769:Methylnitronitrosoguanidine; D008892:Milk / Q000382:microbiology; D009152:Mutagenicity Tests; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008769:Methylnitronitrosoguanidine