v-jun cooperates with v-erbB to transform the thrombocytic/megakaryocytic lineage.
The transforming properties of v-jun, the viral counterpart of the transcription factor AP1, were investigated in avian hematopoietic cells. Two retroviruses, called JB and JBN, expressing both v-jun and v-erbB, were constructed using an avian erythroblastosis-based vector. We show that the cooperative action of both oncogenes allowed the virus to efficiently transform bone marrow cells. No such transformation was obtained with either oncogene alone. JB-transformed bone marrow cells expressed GATA-1, TAL-1, and histone H5, suggesting that they belong to the erythrocytic/thrombocytic lineage. (Thrombocytes are the avian homologues of mammal megakaryocytes.) Moreover, after induction with phorbol 12-myristate 13-acetate JB-transformed bone marrow cells began to differentiate and synthesized high levels of platelet glycoproteins, indicating that they were of thrombocytic origin. These results were confirmed by c-ets1 analysis since this transcription factor, specifically found in cells with megakaryocytic but not erythrocytic features, was clearly detected in these cells.
Garcia
M
M
Ecole Normale Supérieure de Lyon, Laboratoire de Biologie Moléculaire et Cellulaire, Institut National de la Recherche Agronomique, France.
Samarut
J
J
D000818:Animals; D001483:Base Sequence; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D017931:DNA Primers; D005347:Fibroblasts; D016758:Genes, jun; D005914:Globins / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006657:Histones / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism; D008969:Molecular Sequence Data; D016759:Oncogene Protein p65(gag-jun) / Q000235:genetics*; D018739:Oncogene Proteins v-erbB; D009857:Oncogenes; D010980:Platelet Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000235:genetics; D050783:Proto-Oncogene Proteins c-ets; D011519:Proto-Oncogenes; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors
D017931:DNA Primers; D006657:Histones; D016759:Oncogene Protein p65(gag-jun); D018739:Oncogene Proteins v-erbB; D010980:Platelet Membrane Glycoproteins; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; D005914:Globins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D013755:Tetradecanoylphorbol Acetate