title: Aromatase activity in the mare ovary during estrous cycle. Measurement of endogenous steroids and of their in vitro inhibitory effect.
abstract: This present study was undertaken to clarify estrogen synthesis in the mare ovary. First of all, an evaluation of endogenous steroid contents was carried out in the follicular fluid and in the luteal tissue at different stages of the luteal phase. Radioimmunoassays were performed after separation and purification of each hormone by chromatography. High amounts of conjugated (0.9 mg/l) and unconjugated (4 mg/l) estradiol-17 beta were found in the follicular fluid of the large follicules (50 mm). These concentrations of estrogens decreased drastically in the luteal tissue, and only low levels of circulating estrogens are found during the luteal phase. On the other hand, a high aromatization ability has been evidenced in the cyclic corpus luteum in vitro. In an attempt to clarify the regulation of estrogen synthesis, we have tested the inhibitory effect of several endogenous steroids on equine ovarian aromatase activity. 5 alpha-Dihydrotestosterone appeared to be the most potent competitive inhibitor (Ki = 181 nmol/l) of aromatase activity, while the addition of a 3-sulfate group induced a slump in the inhibitory potency of estrone (Ki = 397 nmol/l vs 2206 nmol/l) and dehydroepiandrosterone (Ki = 291 nmol/l vs 6157 nmol/l). The physiological role of these conjugated steroids has not been known until now; we suggest that they would play a role in protecting aromatase from inhibition, in vivo. The high amounts of progesterone found in the luteal tissue (1.3 g/kg of proteins) might play a role in the regulation of estrogen production either by suppressing the induction of aromatase synthesis or by inhibiting the activity of the enzyme complex.
authors.affiliation: Laboratoire de Biochimie, URA Centre National de la Recherche Scientifique 609, Université, Caen, France.
authors.forename: H
authors.initials: H
authors.lastname: Amri
authors.forename: P
authors.initials: P
authors.lastname: Silberzahn
authors.forename: I
authors.initials: I
authors.lastname: al-Timimi
authors.forename: J L
authors.initials: JL
authors.lastname: Gaillard
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D003338:Corpus Luteum / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D004971:Estrus; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D006736:Horses / Q000378:metabolism*; D010053:Ovary / Q000201:enzymology*; D011374:Progesterone / Q000378:metabolism; D011863:Radioimmunoassay; D013256:Steroids / Q000494:pharmacology / Q000502:physiology*
chemical_list: D013256:Steroids; D011374:Progesterone; D004958:Estradiol; D001141:Aromatase