Somatic hypermutations in the immunoglobulin genes of two new human lymphoma lines of lymphatic follicle origin.
Variable immunoglobulin heavy-chain regions (VDJ) of two newly established human lymphoma cell lines (HF-1 and HF-4) were sequenced. The most homologous germline VH gene found for both the HF-1 and HF-4 sequences was VH26 of the VH3a (V gene) family (82% and 91% homologies, respectively). The JH region of the HF-4 heavy-chain sequence contained two nucleotide differences compared to the published germline JH3 gene. The DHJH region of the HF-1 gene had a record high number (20%) of somatic mutations. The numerous hypermutations found in the HF-1 cell line support the hypothesis that in some human follicular lymphomas, mutations continue to accumulate in immunoglobulin genes during the malignant growth. Follicular lymphoma cell lines, which have an active mutational machinery, in future may help to solve the molecular events behind the somatic hypermutations modifying immunoglobulin genes of B lymphocytes.
Wu
H Y
HY
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Tuomikoski
T
T
Eray
M
M
Mattila
P
P
Knuutila
S
S
Kaartinen
M
M
D001483:Base Sequence; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D005803:Genes, Immunoglobulin / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007144:Immunoglobulin J-Chains / Q000235:genetics; D008224:Lymphoma, Follicular; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000302:isolation & purification; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D007143:Immunoglobulin Heavy Chains; D007144:Immunoglobulin J-Chains; D012333:RNA, Messenger