Evidence for production of platelet-activating factor by yeast Saccharomyces cerevisiae cells.
Various yeast strains were screened for platelet-activating factor (PAF) production. High PAF production was found mainly in the strains of Saccharomyces genus. Yeast PAF showed a typical platelet aggregation pattern, which was inhibited by specific PAF antagonists, such as CV-3988, CV-6209 and L-652731. The main molecular species of yeast PAF were identified as 1-palmitoleoyl-, -palmitoyl-, -oleoyl- and -stearoyl-2-acetyl-sn-glycero-3-phosphocholines (16:1, 16:0, 18:1 and 18:0 acylPAFs) and 1-hexadecenyl- and hexadecyl-2-acetyl-sn-glycero-3-phosphocholines (16:1 and 16:0 PAFs), by mass spectrometry. PAF formation in yeast cells increased at the middle stationary phase of growth.
Nakayama
R
R
Department of Food Science, Kyoto Women's University, Japan.
Kumagai
H
H
Saito
K
K
D002855:Chromatography, Thin Layer; D005663:Furans / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D010742:Phospholipid Ethers / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000302:isolation & purification; D010974:Platelet Aggregation; D011498:Protein Precursors / Q000032:analysis; D011726:Pyridinium Compounds / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
D005663:Furans; D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; D011498:Protein Precursors; D011726:Pyridinium Compounds; C053321:CV 6209; C037913:CV 3988; C047504:2,5-bis(3,4,5-trimethoxyphenyl)tetrahydrofuran