title: Light and heavy chains specifying a human IgM kappa autoantibody to a T-cell receptor V beta-antigen.
abstract: Humans frequently produce serum IgM autoantibodies reactive with T-cell receptor beta chains at a determinant defined by peptides corresponding to the first complementarity determining region. It is likely that this determinant serves as a public idiotype involved in immunoregulation. Following screening of culture supernatants from over 60 Epstein-Barr virus-carrying B-cell lines of normal and neoplastic origin, we identified a line, IARC307, that secretes an IgM kappa protein showing marked specificity for the V beta 8.1 CDR1 sequence CKPISGHNSLFQWYRQT. We cloned and sequenced the complete variable regions of the V kappa and VH chains used by the autoantibody. The light chain has a V kappa III sequence related to the 'a' subgroup and uses J kappa 2. The heavy chain has a VHIII sequence essentially identical to the germ-line sequence DP54 and uses the JH6C minigene. The CDR3 is unique, differing from those of other autoantibodies. The antibody is rigorously specific in its specificity for the V beta 8 peptide and does not show polyspecificity for protein or DNA antigens.
authors.affiliation: University of Arizona, College of Medicine, Tucson 85724.
authors.forename: F
authors.initials: F
authors.lastname: Dedeoglu
authors.forename: H
authors.initials: H
authors.lastname: Kaymaz
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: J J
authors.initials: JJ
authors.lastname: Marchalonis
mesh_terms: D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000737:chemistry*; D001483:Base Sequence; D018076:DNA, Complementary / Q000096:biosynthesis; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry*; D007075:Immunoglobulin M / Q000737:chemistry*; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains / Q000737:chemistry*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D018076:DNA, Complementary; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta