Migration of purified embryonic motoneurons grafted into adult mouse CNS.
Embryonic motoneurons were fluorescently-labelled with carbocyanine (diI) by means of retrograde transport and then grafted into the adult mouse spinal cord (L2) and brain (striatum) for 2-10 weeks. The motoneurons were grafted either following purification on the fluorescence-activated cell sorter or in the presence of embryonic glial cells and interneurons from the spinal cord. In both conditions of grafting, motoneurons were found to survive and develop in both grey and white matter and were found to migrate long distances in both regions of the central nervous system. Migration of neurons after grafting remains a controversial issue, therefore we have discussed the work of other groups that have described the same phenomenon.
Ruiz-Flandes
P
P
Department of Pharmacology, Centre Médical Universitaire, Geneva, Switzerland.
Demierre
B
B
Mattenberger
L
L
Kato
A C
AC
D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology; D016380:Brain Tissue Transplantation / Q000502:physiology*; D002232:Carbocyanines; D002465:Cell Movement / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D017690:Cell Transplantation / Q000502:physiology*; D016332:Fetal Tissue Transplantation / Q000502:physiology*; D006651:Histocytochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009046:Motor Neurons / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000254:growth & development
D002232:Carbocyanines; C024286:3,3'-dioctadecylindocarbocyanine