Direct determination of cotinine-N-glucuronide in urine using thermospray liquid chromatography/mass spectrometry.
A thermospray liquid chromatographic/mass spectrometric method has been developed for direct determination of cotinine-N-glucuronide in the urine of smokers. Quantification was performed using methyl-d3-cotinine-N-glucuronide as internal standard and monitoring the protonated aglycons. Using a simple preparation, urine samples from four smokers were analyzed and the results compared favorably with those from a previously reported method that quantifies aglycon release following beta-glucuronidase treatment. Amounts of cotinine-N-glucuronide found in urine from smokers ranged from less than 0.7 to 21 nmol ml-1, indicating wide inter-individual variability in the metabolic production of this metabolite. Cotinine-N-glucuronide was found to be the second most abundant urinary nicotine metabolite. A similar method was developed for trans-3'-hydroxycotinine-N-glucuronide but this compound was not detected in smokers' urine.
Byrd
G D
GD
R.J. Reynolds Tobacco Co., Winston-Salem, NC 27102.
Uhrig
M S
MS
deBethizy
J D
JD
Caldwell
W S
WS
Crooks
P A
PA
Ravard
A
A
Riggs
R m
Rm
D000328:Adult; D002853:Chromatography, Liquid; D003367:Cotinine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D012907:Smoking / Q000652:urine*
C075991:cotinine N-glucuronide; D003367:Cotinine