title: Effects of preinduced Candida-specific systemic cell-mediated immunity on experimental vaginal candidiasis.
abstract: It has been postulated that systemic cell-mediated immunity (CMI) is an important host defense factor against recurrent vaginal infections caused by Candida albicans. Using an estrogen-dependent murine model of vaginal candidiasis, we have previously shown that mice inoculated vaginally with C. albicans acquire a persistent vaginal infection and develop Candida-specific Th1-type systemic CMI. In the present study, experimental vaginitis was monitored in the presence of preinduced systemic Candida-specific CMI. Mice immunized systemically with C. albicans culture filtrate antigens (CaCF) in complete Freund's adjuvant (CFA) had Th1-type reactivity similar to that of vaginally infected mice. CaCF given to mice intravenously induced Candida-specific suppressor T (Ts) cells. Mice preimmunized with CaCF-CFA and given a vaginal inoculum of C. albicans had positive delayed-type hypersensitivity (DTH) reactivity from the time of vaginal inoculation through 4 weeks. Conversely, mice infected in the presence of Ts cells had significantly reduced DTH responses throughout the 4-week period in comparison with naive infected mice. However, the presence of Th1-type Candida-specific DTH cells or Ts cells, either induced in mice prior to vaginal inoculation or adoptively transferred at the time of inoculation, had no effect on the vaginal Candida burden through 4 weeks of infection. A similar lack of effects was obtained in animals with lower Candida population levels resulting from a reduction in or absence of exogenous estrogen. These results suggest that systemic Th1-type CMI demonstrable with CaCF is unrelated to protective events at the level of the vaginal mucosa.
authors.affiliation: Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, Michigan 48201.
authors.forename: P L
authors.initials: PL
authors.lastname: Fidel
authors.forename: M E
authors.initials: ME
authors.lastname: Lynch
authors.forename: J D
authors.initials: JD
authors.lastname: Sobel
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000276:immunology; D002176:Candida albicans / Q000276:immunology*; D002181:Candidiasis, Vulvovaginal / Q000276:immunology*; D005260:Female; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D051379:Mice; D008808:Mice, Inbred CBA; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D000946:Antigens, Fungal; D007376:Interleukin-2; D007371:Interferon-gamma