The corn inhibitor of blood coagulation factor XIIa. Crystallization and preliminary crystallographic analysis.
A 13.6 kDa protein from corn seeds is known to be a highly selective inhibitor of human blood coagulation Factor XIIa (or activated Hageman factor). We have crystallized this inhibitor at 23 degrees C and pH 7.5 from a solution of 30% polyethylene glycol 400, 0.2 M MgCl2, and 0.1 M Hepes. The crystals diffract to at least 2.1 A resolution. The space group is P4(2)2(1)2 with a = b = 57.15 A and c = 80.5 A. The crystals contain 51% solvent. Two heavy atom derivatives have been identified.
Pedersen
L C
LC
Department of Biochemistry, University of Washington, Seattle 98195.
Yee
V C
VC
von Dassow
G
G
Hazeghazam
M
M
Reeck
G R
GR
Stenkamp
R E
RE
Teller
D C
DC
D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D015956:Factor XIIa / Q000037:antagonists & inhibitors*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry; D003313:Zea mays
D011994:Recombinant Proteins; D015956:Factor XIIa