Taxoid side-chain structure determination by electrospray ionization tandem mass spectrometry.
A method to elucidate the side-chain structure of novel taxoids was developed through use of electrospray ionization tandem mass spectrometry. The intact side-chain fragments produced by source fragmentation in the atmosphere-to-vacuum interface were further dissociated in the collision cell of a triple quadrupole mass spectrometer. The distinctive collisionally induced dissociation mass spectra were remarkably informative and gave detailed information on the side-chain functionalities. This technique proved valuable in the characterization of new taxoids produced by yew-tree cell cultures.
Bitsch
F
F
Children's Hospital Oakland Research Institute, CA 94609.
Shackleton
C H
CH
Ma
W
W
Park
G
G
Nieder
M
M
D000470:Alkaloids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry / Q000379:methods*; D017239:Paclitaxel / Q000737:chemistry*
D000470:Alkaloids; D017239:Paclitaxel