Effects of new somatostatin analogs on the cell proliferation of colonic crypts and colonic cancers in rats.
The antiproliferative activity of two new somatostatin (SS) analogs: ASS-51 and ASS-52 have been tested in this study. We assessed their ability to inhibit the DNA synthesis in normal colon crypt cells and in the cells of chemically (dimethylhydrazine)-induced colon cancer in the rats. The incorporation of bromodeoxyuridine (BrDU) into appropriate cell nuclei was used as an index of DNA synthesis. It was found that: 1) Only ASS-51 significantly decreases the colon crypt cell proliferation in the rat when compared to controls. Since both analogs were previously shown to inhibit GH release, these data indicate that the antiproliferogenic effect of ASS-51 is independent of the inhibition of GH release. 2) Both examined analogs did not significantly effect the BrDU incorporation into cell nuclei of chemically-induced colon cancer.
Zerek-Mełeń
G
G
Institute of Endocrinology, Medical University of Lódź, Poland.
Pawlikowski
M
M
Winczyk
K
K
Lachowicz-Ochedalska
A
A
Legowska
A
A
Kwaśny
H
H
Przybylski
J
J
Szadowska
A
A
D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology*; D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003106:Colon / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology*; D007413:Intestinal Mucosa / Q000187:drug effects*; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D013004:Somatostatin / Q000031:analogs & derivatives* / Q000494:pharmacology
C083586:ASS 51; C083587:ASS 52; D013004:Somatostatin