Recombinant human leukemia inhibitory factor is mitogenic for human bone-derived osteoblast-like cells.
Recombinant human leukemia inhibitory factor (rhLIF) is mitogenic for human bone-derived osteoblast-like cells in vitro. [3H]-thymidine incorporation into DNA was stimulated dose-dependently in a prostaglandin E2-independent manner. rhLIF exerted no effect on either the basal or 1,25(OH)2D3-induced synthesis of osteocalcin or alkaline phosphatase (AP) activity. Following treatment with rhLIF, radiolabelled cell nuclei were co-localized with AP, indicating that the mitogenic effect of rhLIF occurs on cells of the osteogenic lineage. Local generation of LIF at the human bone surface may therefore serve as a potential autocrine/paracrine mitogen for progenitor cells of the osteoblastic lineage and so could correlate with the known bone forming properties of LIF.
Evans
D B
DB
Sandoz Pharma AG, Basel, Switzerland.
Gerber
B
B
Feyen
J H
JH
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D002117:Calcitriol / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D008934:Mitogens; D010006:Osteoblasts / Q000166:cytology*; D015675:Osteocalcin / Q000096:biosynthesis; D011994:Recombinant Proteins
D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D008934:Mitogens; D011994:Recombinant Proteins; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D002117:Calcitriol