title: Flap tolerance to ischaemia in streptozotocin-induced diabetes mellitus.
abstract: The effects of diabetes mellitus (DM) on skin flap tolerance to 3 h of secondary venous ischaemia were evaluated. Epigastric island flaps were elevated 3, 6 and 12 weeks after induction of DM in rats. In the non-diabetic control groups, the flap survival was 85% in the 3-week group, 72% in the 6-week, and 78% in the 12-week. In untreated DM groups, the flap survival significantly decreased to 40% in the 3-week group, 25% in the 6-week, and 17% in the 12-week (P < 0.05 in all groups). Flap survival in the DM/insulin group decreased to 31% in the 3-week group. Effects of insulin therapy, however, were observed in later stages of DM: 71% and 62% survival in the 6- and 12-week group, respectively. Significant linear correlations between enzymatic responses and the flap survival were found. The results suggest that DM is detrimental to flap tolerance and is associated with the lack of metabolic responses to secondary ischaemia.
authors.affiliation: Division of Plastic, Reconstructive and Maxillofacial Surgery, Johns Hopkins University School of Medicine, Baltimore.
authors.forename: S
authors.initials: S
authors.lastname: Babovic
authors.forename: M S
authors.initials: MS
authors.lastname: Shin
authors.forename: M F
authors.initials: MF
authors.lastname: Angel
authors.forename: M J
authors.initials: MJ
authors.lastname: Im
authors.forename: C A
authors.initials: CA
authors.lastname: Vander Kolk
authors.forename: P N
authors.initials: PN
authors.lastname: Manson
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007328:Insulin / Q000627:therapeutic use; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply* / Q000201:enzymology / Q000473:pathology; D013524:Surgical Flaps / Q000502:physiology*; D013997:Time Factors; D014022:Tissue Survival / Q000187:drug effects / Q000502:physiology
chemical_list: D001786:Blood Glucose; D007328:Insulin; D005954:Glucosephosphate Dehydrogenase; D005980:Glutathione Reductase; D006593:Hexokinase