title: Temporal pattern of IGF-I expression during mouse preimplantation embryogenesis.
abstract: Although mouse preimplantation embryos express transcripts for the insulin-like growth factor-I receptor (IGF-IR), IGF-I transcripts were not detected by reverse transcription-PCR (RT-PCR) at any stage of preimplantation development in a previous study (Rappolee et al., Genes Dev 6:939-952, 1992). We report that IGF-I transcripts are detected in the preimplantation embryo by RT-PCR and describe the temporal pattern of expression of this transcript from the oocyte to blastocyst stages; the level declines from the oocyte to 8-cell embryo and then increases from the 8-cell to blastocyst stages. An explanation is offered to account for the differences in detecting the IGF-I transcript, and the results are discussed in the context of an autocrine/paracrine IGF-I circuit in the preimplantation mouse embryo.
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia 19104-6018.
authors.forename: A S
authors.initials: AS
authors.lastname: Doherty
authors.forename: G L
authors.initials: GL
authors.lastname: Temeles
authors.forename: R M
authors.initials: RM
authors.lastname: Schultz
mesh_terms: D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D002455:Cell Division; D017931:DNA Primers; D005260:Female; D015870:Gene Expression; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I