Streptozotocin-induced diabetes increases fructose 2,6-bisphosphate levels and glucose metabolism in rat macrophages.
The acute effects of streptozotocin-induced diabetes on several parameters of glucose metabolism were investigated in rat peritoneal macrophages. These cells accumulated in vitro about twofold more fructose 2,6-bisphosphate in the presence of increasing glucose concentration than cells from normal rats, and an increased production of lactate was observed. Phosphofructokinase-1, phosphofructokinase-2, hexokinase, and pyruvate kinase activities were increased in cells from diabetic rats compared with those from normal rats. Transport of 2-deoxy-D-glucose was increased in cells from diabetic rats. [U-14C]Glucose incorporation into glycogen was also increased in cells from diabetics and the 14CO2 liberation was less than in cells from normal animals. Moreover, macrophages from diabetics did not possess a more active pentose phosphate pathway (measure with [1-14C]glucose oxidation) nor a greater production of superoxide anion (index of activation of macrophages) than in cells from normal animals.
Bustos
R
R
Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina, Universidad de Sevilla, Spain.
Moreno-Aurioles
V R
VR
Conde
M
M
Montaño
R
R
Sobrino
F
F
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005635:Fructosediphosphates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
D005635:Fructosediphosphates; D013311:Streptozocin; C027652:fructose 2,6-diphosphate; D005947:Glucose