Spontaneous formation of small sized albumin/acacia coacervate particles.
Microgel coacervate particles form spontaneously on mixing aqueous solutions of oppositely charged albumin and acacia, under specific conditions of pH, ionic strength, and polyion concentration, close to but not at the optimum conditions for maximum coacervate yield. The mean particle diameter of these coacervate particles is approximately 6 microns when suspended in aqueous media, as determined by HIAC/Royco particle analysis. The geometric standard deviation of the particles falls in the range 1.2-1.9 microns. The particle size was not dependent on the method of emulsification of the coacervate in the equilibrium phase, or on the stirring speed applied during the manufacturing process. The microgel particles were stable on storage, for periods up to forty-six days, without the addition of a chemical cross-linking agent, or the application of heat. Stability was measured with respect to the change in particle size of samples stored at different temperatures. The non-cross-linked microcapsules were also shown to be stable on pH change, to pH values outside the coacervation pH range. At the optimum conditions for maximum coacervate yield the albumin/acacia system formed a very viscous coacervate phase, which was unsuitable for microcapsule preparation. The rheological properties of albumin/acacia and gelatin/acacia complex coacervates optimized for maximum coacervate yield were compared. The albumin/acacia coacervate was shown to be three orders of magnitude more viscous than the gelatin/acacia system.
Burgess
D J
DJ
Department of Pharmaceutics, College of Pharmacy, University of Illinois, Chicago 60612.
Singh
O N
ON
D002626:Chemistry, Pharmaceutical; D003102:Colloids; D003432:Cross-Linking Reagents; D004339:Drug Compounding; D005782:Gels; D005976:Glutaral / Q000737:chemistry; D006170:Gum Arabic / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013696:Temperature; D014783:Viscosity
D003102:Colloids; D003432:Cross-Linking Reagents; D005782:Gels; D012710:Serum Albumin, Bovine; D006170:Gum Arabic; D005976:Glutaral