title: A purine-rich exon sequence enhances alternative splicing of bovine growth hormone pre-mRNA.
abstract: A previous study has demonstrated that deletion of a region within the last exon of bovine growth hormone (bGH) pre-mRNA results in almost complete retention of the upstream intron (Hampson, R. K., LaFollette, L., and Rottman, F. M. (1989) Mol. Cell. Biol. 9, 1604-1610). We now demonstrate that insertion of a simple purine-rich element (GGAAG), which is present within the deleted region, activates intron splicing upon expression in transfected cells. Moreover, several repeats of the GGAA(G) sequence restore splicing to near wild-type levels and direct the binding of a factor present in HeLa cell nuclear extracts. Mutation of the 5'-splice site toward U1 small nuclear RNA complementarity eliminates dependence on the downstream exon sequence for splicing. These results support a model for alternative intron retention in which purine-rich sequences function as part of an "exonic splicing enhancer" to complement a weak 5'-splice site and thereby facilitate intron removal. As a result, the majority of bGH mRNA is processed to remove intron D while still allowing a fraction of bGH mRNA containing the intact intron to reach the cytoplasm.
authors.affiliation: Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4960.
authors.forename: W P
authors.initials: WP
authors.lastname: Dirksen
authors.forename: R K
authors.initials: RK
authors.lastname: Hampson
authors.forename: Q
authors.initials: Q
authors.lastname: Sun
authors.forename: F M
authors.initials: FM
authors.lastname: Rottman
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002417:Cattle; D006224:Cricetinae; D004742:Enhancer Elements, Genetic; D005091:Exons; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009838:Oligodeoxyribonucleotides; D011687:Purines; D012322:RNA Precursors / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection
chemical_list: D009838:Oligodeoxyribonucleotides; D011687:Purines; D012322:RNA Precursors; D012342:RNA, Small Nuclear; D013006:Growth Hormone