title: One-lane chemical sequencing of 3'-fluorescent-labeled DNA.
abstract: A method for DNA sequencing that combines limited chemical degradation of 3'-fluorescent-labeled DNA with densitometric analysis using an automatic sequencer is presented. The DNA sequence is determined in a single electrophoretic lane by monitoring the intensities of bands representing products of cleavage at the four bases obtained by solvolysis in hot aqueous piperidine (10%) followed by treatment with hot formamide. An application of the method for the detection of point mutations is reported.
authors.affiliation: Dipartimento di Scienze Biomediche e Biotechnologie, Università di Brescia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Ferraboli
authors.forename: R
authors.initials: R
authors.lastname: Negri
authors.forename: E
authors.initials: E
authors.lastname: Di Mauro
authors.forename: S
authors.initials: S
authors.lastname: Barlati
mesh_terms: D001322:Autoanalysis; D001483:Base Sequence; D003199:Computer Systems; D004247:DNA / Q000737:chemistry*; D003720:Densitometry; D005453:Fluorescence; D005559:Formamides; D007834:Lasers; D008969:Molecular Sequence Data; D010880:Piperidines; D017354:Point Mutation
chemical_list: D005559:Formamides; D010880:Piperidines; C031066:formamide; C032727:piperidine; D004247:DNA