title: Determination of ovarian cycle in Goeldi's monkey (Callimico goeldii) via the measurement of steroids and peptides in plasma and urine.
abstract: Goeldi's monkey (Callimico goeldii) is an endangered species of New World primate. The present study provides the first description of the non-conception ovarian cycle in this species based on circulating reproductive steroid and peptide hormones. The data obtained were used to validate a non-invasive system for monitoring cyclicity based on urinary reproductive steroid metabolites. Nine sexually mature females were studied. In three females, matched blood and urine samples were collected once every 2-3 days for 90-120 days; in three other females, matched blood and urine samples were collected daily for 14-20 days for one peri-ovulatory period; and in the remaining three females, urine samples only were collected once every 1-3 days for 40-60 days. Plasma progesterone, oestrone-3-conjugates and bioactive LH were measured, in addition to urinary pregnanediol-3 alpha-glucuronide and oestrone-3-conjugates. The mean maximum concentration of plasma LH occurred 1-2 days before a significant rise in plasma progesterone, which was considered to occur 1 day after ovulation. On the basis of plasma progesterone titres, the duration of the ovarian cycle was estimated as 23.9 +/- 0.4 days (n = 9), and constituted a follicular phase of 10.7 +/- 0.3 days and a luteal phase of 13.5 +/- 0.3 days. Urinary pregnanediol-3 alpha-glucuronide demonstrated a high correlation with plasma progesterone (r = 0.8), and demonstrated a significant rise at the same time as plasma progesterone.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Anthropologisches Institut, Universität Zürich-Irchel, Switzerland.
authors.forename: C R
authors.initials: CR
authors.lastname: Pryce
authors.forename: M
authors.initials: M
authors.lastname: Jurke
authors.forename: H J
authors.initials: HJ
authors.lastname: Shaw
authors.forename: I G
authors.initials: IG
authors.lastname: Sandmeier
authors.forename: M
authors.initials: M
authors.lastname: Doebeli
mesh_terms: D000818:Animals; D016647:Callimico / Q000502:physiology*; D004966:Estrogens, Conjugated (USP) / Q000097:blood / Q000652:urine; D004971:Estrus / Q000502:physiology*; D005260:Female; D005498:Follicular Phase / Q000502:physiology; D008183:Luteal Phase / Q000502:physiology; D007986:Luteinizing Hormone / Q000097:blood; D010061:Ovulation Detection; D011276:Pregnanediol / Q000031:analogs & derivatives* / Q000652:urine; D011374:Progesterone / Q000097:blood*
chemical_list: D004966:Estrogens, Conjugated (USP); C022270:pregnanediol-3 alpha-glucuronide; D011374:Progesterone; D007986:Luteinizing Hormone; D011276:Pregnanediol