Polypyrimidine/polypurine sequence in plasmid DNA enhances formation of dimer molecules in Escherichia coli. Dimerization of plasmid DNA in Escherichia coli.
Formation of dimer molecules of a recombinant plasmid, pTIR10, which carries a pyrimidine/purine-biased stretch occurs about 6-fold more efficiently than for the control plasmid pUC19 in Escherichia coli strain JM107. Since pyrimidine/purine-biased sequences have a potential to form unusual DNA structures, this observation suggests that the inserted sequence affects the replication process of plasmid DNA, probably by forming a triple helix under physiological conditions.
Kato
M
M
Department of Life Science, College of Integrated Arts and Sciences, University of Osaka Prefecture, Sakai, Japan.
D000818:Animals; D001483:Base Sequence; D004261:DNA Replication; D004274:DNA, Recombinant / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011687:Purines / Q000737:chemistry; D011740:Pyrimidine Dimers / Q000378:metabolism*; D011743:Pyrimidines / Q000737:chemistry; D051381:Rats; D011995:Recombination, Genetic
D004274:DNA, Recombinant; D011687:Purines; D011740:Pyrimidine Dimers; D011743:Pyrimidines