Single-channel and whole-cell recordings from on-neurone glial cells in Helix pomatia ganglia.
A procedure is described for performing patch-clamp recordings on satellite glial cells kept in place within the nervous ganglia in the mollusc Helix. Glial cell properties were deduced from whole-cell and cell-attached recordings. The glial membrane was found to contain densely packed inwardly rectifying K+ channels. Activation of the neurones, under either current-clamp or voltage-clamp conditions, depolarized the glial cell layer wrapped around the neurones and induced a delayed persistent increase in the K+ channel opening probability. These results suggest that the glial channels opened in response to a signal emanating from the active neurones. This preparation provides a useful means of detecting and analysing neurone-glial interactions at the cell and unitary channel levels.
Gommerat
I
I
Laboratoire de Neurobiologie, CNRS, Marseille, France.
Jacquet
G
G
Chagneux
H
H
Gola
M
M
D000818:Animals; D002450:Cell Communication; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D005724:Ganglia / Q000502:physiology*; D006372:Helix, Snails; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D008564:Membrane Potentials; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009457:Neuroglia / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D015221:Potassium Channels / Q000502:physiology*
D015221:Potassium Channels