Two tyrosinase nonapeptides recognized on HLA-A2 melanomas by autologous cytolytic T lymphocytes.
A number of cytolytic T lymphocyte (CTL) clones derived from several melanoma patients have been found to recognize a majority of melanomas from HLA-A2 patients. We have reported previously that two such CTL clones recognize a product of the tyrosinase gene that is presented by HLA-A2. Here we show that one of these CTL clones recognizes a peptide encoded by the first nine amino acids of the putative signal sequence of tyrosinase. The other CTL clone recognizes a different tyrosinase peptide corresponding to amino acids 368-376. Both peptides contain consensus motifs of HLA-A2 binding peptides.
Wölfel
T
T
I. Medizinische Klinik und Poliklinik, Johannes Gutenberg-Universität, Mainz, FRG.
Van Pel
A
A
Brichard
V
V
Schneider
J
J
Seliger
B
B
Meyer zum Büschenfelde
K H
KH
Boon
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003602:Cytotoxicity, Immunologic; D017931:DNA Primers / Q000737:chemistry; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008545:Melanoma / Q000276:immunology*; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D011994:Recombinant Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D015789:HLA-A2 Antigen; D010455:Peptides; D011994:Recombinant Proteins; D014442:Monophenol Monooxygenase