An investigation into the use of restriction endonuclease analysis for the study of transmission of Actinomyces.
DNA fingerprints of 28 reference strains of Actinomyces, comprising representatives of different species and serotypes, and 19 isolates recovered from 16 periodontal patients was performed. The aim was to determine the potential of the method for detecting strain differences in terms of discriminatory power and to evaluate its usefulness in the typing of Actinomyces strains for eco-epidemiological studies. Among the 17 restriction endonucleases tested, Bst EII, Pvu II and Sma I proved to be the most suitable for the genus Actinomyces restriction digest analysis. Visual comparisons of Bst EII, Pvu II and Sma I digest patterns of chromosomal DNA revealed clear differences within species but also within serotypes of Actinomyces that are otherwise identical. The method offers the qualities for use as an epidemiological tool for identifying sources and tracing routes of transmission of Actinomyces: stability, reproducibility, ease of preparation and interpretation and enough sensitivity for detection of differences between morphologically and serologically similar strains of Actinomyces.
Barsotti
O
O
Laboratoire de Microbiologie Orale, UFR d'Odontologie, Lyon, France.
Morrier
J J
JJ
Decoret
D
D
Benay
G
G
Rocca
J P
JP
D000190:Actinomyces / Q000145:classification* / Q000235:genetics*; D000196:Actinomycosis / Q000453:epidemiology / Q000635:transmission*; D000818:Animals; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D006801:Humans; D010518:Periodontitis / Q000453:epidemiology / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004262:DNA Restriction Enzymes