Experimental models for optimization of insulin separation on reversed phase columns.
Experimentally defined relationships have been found to describe adequately the retention and separation of bovine and porcine insulins as well as their desamido products on reversed phases C4 30 nm and C18 10 nm pore diameter. The equations are valid for a region of initial acetonitrile concentrations from 16 to 31% and gradient rates from 0.04 to 0.60%/min. The peak heights showed an exact non-linear relationship with the time interval between the first and last peak of interest, independent of their retention time. The number of experiments required for obtaining the parametric estimates of the models depends on the particular task, but in all cases is less than eight. The relationships found permit the correct choice to be made in advance both for analysis conditions for a particular column and for different requirements of insulin analysis.
Dimov
N
N
NIHFI, Sofia, Bulgaria.
Simeonov
S
S
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003198:Computer Simulation; D007328:Insulin / Q000302:isolation & purification*; D008433:Mathematics; D013552:Swine
D007328:Insulin