title: A functional test for maternally inherited cadherin in Xenopus shows its importance in cell adhesion at the blastula stage.
abstract: We report here on the consequences of reducing the expression of EP-cadherin at the earliest stages of Xenopus development. Injection of oligodeoxynucleotides antisense to maternal EP-cadherin mRNA into full-grown oocytes reduced the mRNA level in oocytes, and the protein level in blastulae. Adhesion between blastomeres was significantly reduced, as seen in whole embryos, and in assays of the ability of blastomeres to reaggregate in culture. This effect was especially conspicuous in the inner cells of the blastula and included the disruption of the blastocoel. The severity of the EP-cadherin mRNA depletion and of the disaggregation phenotype was dose dependent. This phenotype was rescued by the injection into EP-cadherin mRNA-depleted oocytes of the mRNA coding for a related cadherin, E-cadherin, that is normally expressed at the gastrula stage in the embryonic ectoderm.
authors.affiliation: Wellcome/CRC Institute, Cambridge, UK.
authors.forename: J
authors.initials: J
authors.lastname: Heasman
authors.forename: D
authors.initials: D
authors.lastname: Ginsberg
authors.forename: B
authors.initials: B
authors.lastname: Geiger
authors.forename: K
authors.initials: K
authors.lastname: Goldstone
authors.forename: T
authors.initials: T
authors.lastname: Pratt
authors.forename: C
authors.initials: C
authors.lastname: Yoshida-Noro
authors.forename: C
authors.initials: C
authors.lastname: Wylie
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000648:ultrastructure; D015152:Blotting, Northern; D015820:Cadherins / Q000235:genetics / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012333:RNA, Messenger; D029867:Xenopus Proteins; D014982:Xenopus laevis
chemical_list: C074260:CDH3 protein, Xenopus; D015820:Cadherins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D029867:Xenopus Proteins