Differential capacity of various anti-Ig reagents to synergize with interleukin-4 to induce clonal expansion in resting and activated B cells.
The regulation of B cell growth responses by cross-linking of surface Ig receptors by monoclonal or polyclonal antibodies and its interaction with interleukin-4 (IL-4) has been evaluated. High-density B cells from autoimmune NZB/W mice were proliferated in the presence of low concentrations of a polyclonal goat anti-mu antibody and IL-4. Similarly, slight but significant positive responses were obtained with anti-K monoclonal antibodies and IL-4. In contrast, the same concentration of other surface Ig binding agents such as monoclonal antibodies against mu and delta immunoglobulin heavy chains did not synergize with IL-4 in order to induce clonal expansion of resting B cells. Polyclonal and monoclonal anti-Ig reagents inhibited the spontaneous cell growth of low-density B cells (in vivo activated B cells), although the negative effect was less noticeable with anti-delta monoclonal antibodies. Upon addition of IL-4 to activated B cell cultures in the presence of goat anti-mu antibody, a significant proliferative response was obtained. However, this lymphokine was unable to induce clonal expansion of B cell blasts in the presence of the various monoclonal anti-Ig antibodies employed in this study. Similar results were obtained with splenic B cells from normal animals, suggesting that the results shown in this report are not due to putative functional peculiarities of autoimmune B cells.
Gutiérrez
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C
Department of Immunology, Hospital Central de Asturias, Oviedo, Spain.
Cabrero
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E
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005260:Female; D007136:Immunoglobulins / Q000187:drug effects / Q000276:immunology*; D015847:Interleukin-4 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008814:Mice, Inbred NZB; D008815:Mice, Inbred Strains
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D007136:Immunoglobulins; D015847:Interleukin-4