An analysis of actin isoforms expressed in hair-cell enriched fractions of the chick basilar papilla by the polymerase chain reaction technique.
Actin mRNA was characterised in hair-cell enriched fractions of the chick basilar papilla, by means of the reverse-transcription polymerase chain reaction technique. Primers were directed against the 3' untranslated portions of the actin mRNAs. Evidence for beta-cytoplasmic and gamma-cytoplasmic actin mRNA was found; no evidence was found for alpha-skeletal, alpha-cardiac or type 5 cytoplasmic actin mRNAs. Since beta-actin is known to form bundles of filaments whereas gamma-actin does not, this suggests that the hair-cell stereocilia are composed of beta-actin.
Pickles
J O
JO
Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia.
D000199:Actins / Q000032:analysis* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001489:Basilar Membrane / Q000737:chemistry*; D002645:Chickens; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D006198:Hair Cells, Auditory / Q000737:chemistry*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D013237:Stereoisomerism
D000199:Actins; D017931:DNA Primers; D012333:RNA, Messenger