[The modelling of the genetic correction of familial hypercholesterolemia by creating cellular clones stably expressing the human low-density lipoprotein receptor].
The stable expression of human low-density lipoprotein receptor (rLDL) was studied in Chinese hamster ovary (CHO) cells transformed by the pMSVL recombinant plasmid. This plasmid contains full-length rLDL cDNA and hybrid promoter consisting of regulatory elements from early SV-40 promoter and glucocorticoid responsive enhancer of Moloney sarcoma virus. The expression of rLDL cDNA in transformed cells was demonstrated using RNA-cDNA blot hybridization, PCR analysis, and immunofluorescent analysis.
Dolzhanskaia
N B
NB
Patkin
E L
EL
Runova
O L
OL
Shvartsman
A L
AL
Gaĭtskhoki
V S
VS
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D006224:Cricetinae; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000235:genetics*; D008957:Models, Genetic; D010053:Ovary; D016133:Polymerase Chain Reaction; D011973:Receptors, LDL / Q000032:analysis / Q000235:genetics*; D014170:Transformation, Genetic
D011973:Receptors, LDL
Modelirovanie geneticheskoĭ korrektsii semeĭnoĭ giperkholesterinemii putem sozdaniia kletochnykh klonov, stabil'no ékspressiruiushchikh retseptor lipoproteinov nizkoĭ plotnosti cheloveka.