Carnocin UI49, a potential biopreservative produced by Carnobacterium piscicola: large scale purification and activity against various gram-positive bacteria including Listeria sp.
This paper describes a simple purification method for the purification of carnocin UI49, a potential biopreservative produced by Carnobacterium piscicola UI49. The protocol was also applicable for the isolation of nisin Z, which is a biopreservative produced by Lactococcus lactis SIK-83. The protocol consists of only two purification steps, XAD chromatography and cation exchange chromatography. It is quick, easy, and can be used for large scale purification of these lantibiotics. The bactericidal activity of carnocin UI49 against carnobacteria, lactococci and Listeria was compared with that of nisin Z. The carnobacteria showed similar sensitivity towards carnocin UI49 and nisin. The nisin producing L. lactis strains were very sensitive towards carnocin UI49, while the non-producing L. lactis strains were more sensitive to nisin. The Listeria strains were weakly sensitive to carnocin UI49, lower concentrations of nisin were needed to inhibit growth.
Stoffels
G
G
Science Institute, University of Iceland, Reykjavík.
Sahl
H G
HG
Gudmundsdóttir
A
A
D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D005520:Food Preservatives / Q000302:isolation & purification / Q000494:pharmacology*; D006093:Gram-Positive Asporogenous Rods / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D007777:Lactobacillaceae / Q000187:drug effects / Q000378:metabolism; D013294:Lactococcus lactis / Q000187:drug effects / Q000378:metabolism; D008087:Listeria / Q000187:drug effects*; D009561:Nisin / Q000302:isolation & purification / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D005520:Food Preservatives; D009561:Nisin