Determination of albendazole-2-aminosulfone in bovine milk using high-performance liquid chromatography with fluorometric detection.
An analytical method for the determination of albendazole (ABZ) residues in bovine milk was developed using one of its major metabolites, albendazole-2-aminosulfone (ABZ2NH2) as the marker. The method involved acid hydrolysis of milk followed by liquid-liquid extraction and solid-liquid phase clean-up of the extract. A reversed-phase HPLC with fluorometric detection was used to quantitate the marker residue. The method exhibited a high degree of precision and good accuracy as demonstrated by a relative standard deviation (R.S.D.) < 5% for the replicate analyses and 91.8 to 104.1% recovery of the fortification level (25-200 ng/ml), respectively. The ratio of the concentrations of the marker and total residues in milk over a 36-120 h withdrawal period was found to be steady at 43.1 indicating a definite relationship between the marker and the total residues of ABZ. The analytical method was used successfully to determine total residues in milk of cattle treated with ABZ.
Chu
P S
PS
Department of Environmental Safety and Mass Spectrometry, SmithKline Beecham Animal Health, West Chester, PA 19380.
Wang
R Y
RY
Brandt
T A
TA
Weerasinghe
C A
CA
D015766:Albendazole / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D000871:Anthelmintics; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004350:Drug Residues / Q000032:analysis*; D005260:Female; D005506:Food Contamination; D008892:Milk / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000871:Anthelmintics; C085926:albendazole-2-aminosulfone; D015766:Albendazole