Inhibitory effects of benzyl isothiocyanate and benzyl thiocyanate on diethylnitrosamine-induced hepatocarcinogenesis in rats.
The effects of two aromatic thiocyanates, benzyl isothiocyanate (BITC) and benzyl thiocyanate (BTC), on diethylnitrosamine (DEN)-induced hepatocarcinogenesis were examined in rats. A total of 108 male ACI/N rats, 5 weeks old, were divided into 6 groups (18 rats in each). Group 1 was given a single i.p. injection of DEN (200 mg/kg body weight) one week after the start of the experiment and then kept on the basal diet until the end of the experiment (1 year). Groups 2 and 3 were treated with DEN and received dietary BITC (100 ppm) or BTC (100 ppm), respectively, throughout the experimental duration. Groups 4 and 5 were not given the carcinogen and were fed the diet containing BITC or BTC, respectively. Group 6 was kept on the basal diet alone and served as a control. Liver neoplasms were seen in Groups 1, 2 and 3. Incidence and average number of liver neoplasms in Group 2 were significantly smaller than in Group 1 (P < 0.0005 and P < 0.001, respectively). The incidence of liver neoplasms in Group 3 was slightly lower than in Group 1, although the difference was not statistically significant. The numbers of glutathione S-transferase placental form (GST-P)-positive foci in Group 2 and gamma-glutamyltranspeptidase (GGT)-positive foci in Groups 2 and 3 were significantly smaller than those in Group 1 (P < 0.001). The average and unit areas of GST-P- or GGT-positive foci in Group 2 or 3 were also significantly smaller than those in Group 1 (P < 0.05). These results suggest that BITC and BTC are chemopreventive agents for DEN-induced liver tumorigenesis.
Sugie
S
S
Department of Pathology, Gifu University School of Medicine.
Okumura
A
A
Tanaka
T
T
Mori
H
H
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D004052:Diethylnitrosamine; D005982:Glutathione Transferase / Q000032:analysis; D017879:Isothiocyanates / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013861:Thiocyanates / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000032:analysis
D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D013861:Thiocyanates; D004052:Diethylnitrosamine; C031403:benzyl isothiocyanate; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase; C034658:benzyl thiocyanate