A liquid chromatographic method for the determination of fenoprofen in equine plasma and urine.
A high performance liquid chromatographic method to measure plasma and urine fenoprofen levels in equine biofluids is described. Liquid-liquid extraction with diethylether was used to isolate the drug from plasma and urine. The accuracy and reproducibility of the method were within acceptable limits over the concentration range 0-10 micrograms/mL and 0-20 micrograms/mL respectively from plasma and urine. Detection limits were 0.05 microgram/mL (2 mL plasma) and 0.2 microgram/mL (0.5 mL urine). This procedure was applied to ascertain the pharmacokinetics of a 3 g dose of fenoprofen calcium in a horse.
Delbeke
F T
FT
Faculteit Diergeneeskunde, Universiteit Gent, Belgium.
Debackere
M
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D005260:Female; D005279:Fenoprofen / Q000097:blood* / Q000652:urine*; D006736:Horses / Q000097:blood* / Q000652:urine*; D015203:Reproducibility of Results
D005279:Fenoprofen