Polyhedral assembly of a membrane protein in its three-dimensional crystal.
A novel ordered assemblage of bacteriorhodopsin, a transmembrane protein functioning as a light-driven proton pump, is found in its three-dimensional crystal. Atomic force microscope images of the crystal surface reveal that spherical protein clusters with a diameter of approximately 50 nm are hexagonally close-packed. Electron micrographs of mechanically disintegrated crystals show that the inside of the protein cluster is filled with the mother liquor. The crystal is made up of hollow protein clusters. When disintegrated crystals are illuminated in the presence of a lipophilic anion, a significant alkalization of the external medium occurs. This result indicates that the protein cluster contains native lipids and that the cytoplasmic side of the protein faces the external medium. X-ray diffraction patterns and the observed diameter of the spherical shell suggest that approximately 200 bacteriorhodopsin trimers are aligned on a polyhedral surface lattice. Another remarkable feature of the spherical assemblies of bacteriorhodopsin is that they fuse with each other at low ionic strength and occasionally form a tubular or doughnut-like structure. The concept of membrane protein polymorphism is introduced on the basis of these observations, and it is used to describe the dynamic structure of some other biological membranes.
Kouyama
T
T
Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Yamamoto
M
M
Kamiya
N
N
Iwasaki
H
H
Ueki
T
T
Sakurai
I
I
D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D003460:Crystallization; D018360:Crystallography, X-Ray; D019617:Halobacterium salinarum / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation
D008051:Lipid Bilayers; D008565:Membrane Proteins; D011108:Polymers; D001436:Bacteriorhodopsins