title: On the rapid deprotection of synthetic oligonucleotides and analogs.
abstract: The efficiency of oligodeoxynucleotide deprotection is greatly enhanced using a combination of: (a) ethanolamine, and especially a mixture of hydrazine, ethanolamine and methanol, in place of the usual aqueous ammonia; (b) tert-butylphenoxyacetyl amino protecting groups, and (c) oxalyl link between the first nucleotide and the polymeric support. The extent of base modification, particularly of C, is shown to be extremely low, and the quality of deprotected oligonucleotides is as high as in the case of ammonia deprotection. This method is also shown to be applicable to the preparation of phosphorothioate and methylphosphonate oligodeoxynucleotides and oligoribonucleotides.
authors.affiliation: Pharmacology Department, Georgetown University Medical Center, Washington, DC 20007.
authors.forename: N N
authors.initials: NN
authors.lastname: Polushin
authors.forename: A M
authors.initials: AM
authors.lastname: Morocho
authors.forename: B C
authors.initials: BC
authors.lastname: Chen
authors.forename: J S
authors.initials: JS
authors.lastname: Cohen
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D019856:Ethanolamine; D004983:Ethanolamines / Q000737:chemistry; D006834:Hydrazines / Q000737:chemistry; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis*; D016133:Polymerase Chain Reaction; D013873:Thionucleotides / Q000138:chemical synthesis; D013997:Time Factors
chemical_list: D004983:Ethanolamines; D006834:Hydrazines; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D013873:Thionucleotides; C021992:dideoxyribonucleoside methylphosphonates; C029424:hydrazine; D019856:Ethanolamine