The Asc locus for resistance to Alternaria stem canker in tomato does not encode the enzyme aspartate carbamoyltransferase.
The fungal disease resistance locus Alternaria stem canker (Asc) in tomato has been suggested to encode the enzyme aspartate carbamoyltransferase (ACTase). To test this hypothesis a segment of the tomato ACTase gene was amplified by the polymerase chain reaction (PCR) using degenerate primers. The PCR product obtained was subsequently used to isolate an ACTase cDNA clone. Restriction fragment length polymorphism (RFLP) linkage analysis showed that the ACTase gene and the Asc locus do not cosegregate. RFLP mapping positioned the ACTase gene on chromosome 11, while the Asc locus is located on chromosome 3. These results exclude the possibility that the ACTase protein is encoded by the Asc locus.
Overduin
B
B
Department of Genetics, Vrije Universiteit Amsterdam, The Netherlands.
Hogenhout
S A
SA
van der Biezen
E A
EA
Haring
M A
MA
Nijkamp
H J
HJ
Hille
J
J
D000528:Alternaria / Q000276:immunology; D000595:Amino Acid Sequence; D001221:Aspartate Carbamoyltransferase / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D004277:DNA, Single-Stranded; D017343:Genes, Plant / Q000235:genetics* / Q000276:immunology; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004277:DNA, Single-Stranded; D001221:Aspartate Carbamoyltransferase