Characterization of a weak allele of the GL1 gene of Arabidopsis thaliana.
A genomic clone containing the gl1-2 allele has been isolated and sequenced. The predicted amino acid sequence of the gl1-2 protein is identical to that of the GL1-Col allele up to position 201. At this point in the coding region of gl1-2 there is a deletion relative to the wild-type sequence that results in an in-frame stop codon at position 202. This deletion removes 27 amino acid residues, including a highly negatively charged region, from the predicted gl1-2 polypeptide. The loss of this negatively charged carboxy-terminal region from the gl1-2 product is most likely the cause of the partial loss of gene activity which results in a reduction in leaf trichome initiation.
Esch
J J
JJ
School of Biological Sciences, University of Nebraska-Lincoln, NE 68588-0118.
Oppenheimer
D G
DG
Marks
M D
MD
D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000648:ultrastructure; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004268:DNA-Binding Proteins; D005091:Exons; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D010940:Plant Proteins; C071556:GL1 protein, Arabidopsis; D004247:DNA