Nucleotide sequence and expression of a gentamicin resistance gene isolated from the R plasmid in Serratia marcescens.
The 1.0 kb fragment carrying a gentamicin resistance determinant was subcloned from the R plasmid in clinical isolates of Serratia marcescens and its nucleotide sequence was determined. It contains an open reading frame composed of 858 nucleotides, which shows 97% homology to the aacC2 gene in nucleotide sequence. Minicells carrying the 1.0 kb insert DNA on the plasmids produced a 30 kd protein that was consistent with the expected size of the deduced amino acid sequence from the open reading frame. A search for the promoter revealed a putative promoter of which the -35 region was provided by the right end of Tn3, suggesting that the expression of the gentamicin resistance gene is dependent on the Tn3 sequence.
Jung
J S
JS
Department of Biology, Sunchon National University, Korea.
Cheong
T C
TC
Cho
M S
MS
Hah
Y C
YC
Chung
J H
JH
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects; D005798:Genes, Bacterial; D005839:Gentamicins / Q000633:toxicity*; D008715:Methionine / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011815:R Factors; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012706:Serratia marcescens / Q000187:drug effects / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005839:Gentamicins; D008715:Methionine