The firing rates of human motoneurones voluntarily activated in the absence of muscle afferent feedback.
1. To quantify the net influence of muscle afferent feedback on the firing rates of human motoneurones, the discharge frequencies of single motor axons in the common peroneal nerve were recorded during sustained voluntary efforts performed in the absence of feedback from the target muscle. These data were compared with the firing rates of single motor units in the intact tibialis anterior muscle. In five subjects, recordings were made from fifty-two motor axons innervating tibialis anterior during acute deafferentation and paralysis of the dorsiflexor muscles produced by anaesthetic block of the nerve distal to the recording site. 2. Maximal sustainable firing rates were determined for twenty-four motoneurons, twelve of which were classified as relatively low threshold (estimated recruitment level < or = 10% maximal) and six as high threshold. Mean firing rates of the low-threshold motoneurones (21.7 +/- 2.7 Hz; +/- S.E.M.) were significantly higher than those of the high-threshold motoneurones (14.0 +/- 4.4 Hz). The mean firing rate of the twenty-four deafferented motoneurones during maximal efforts to contract the paralysed muscle was 18.6 +/- 1.9 Hz, significantly lower than the maximal firing rates of single motor units recorded from the normally innervated tibialis anterior muscle (28.2 +/- 0.6 Hz). 3. During half-maximal efforts, the mean firing rate of eight deafferented motoneurones (10.8 +/- 1.1 Hz) was significantly lower than that of intact motor units (16.5 +/- 0.2 Hz). A similar finding was apparent during minimal efforts; the mean discharge frequency of seven deafferented motoneurones during weak voluntary efforts was 6.0 +/- 0.9 Hz, compared with 7.3 +/- 0.13 Hz for intact motor units. Overall, the range of motoneurone firing rates (from minimal to maximal levels of voluntary effort) was significantly affected by the acute deafferentation, but was shifted significantly to lower rates. 4. During sustained maximal voluntary efforts of at least 30 s duration the firing rate of deafferented motoneurones decreased over the first 5 s but was then maintained, i.e. there was no progressive decline as occurs with normally innervated motor units during fatiguing contractions. This observation supports a reflex origin for the normal decline in motoneurone discharge. 5. It is concluded that muscle afferents in the common peroneal nerve provide a net facilitation to the tibialis anterior motoneurone pool, reflexly increasing the motor output at all levels of voluntary drive by approximately one-third.
Macefield
V G
VG
Department of Clinical Neurophysiology, Prince Henry Hospital and Prince of Wales Medical Research Institute, University of New South Wales, Sydney, Australia.
Gandevia
S C
SC
Bigland-Ritchie
B
B
Gorman
R B
RB
Burke
D
D
D000200:Action Potentials / Q000502:physiology; D000293:Adolescent; D000328:Adult; D000344:Afferent Pathways / Q000502:physiology; D004594:Electrophysiology; D005246:Feedback / Q000502:physiology; D006801:Humans; D007866:Leg; D008297:Male; D009046:Motor Neurons / Q000502:physiology*; D009119:Muscle Contraction / Q000502:physiology; D009121:Muscle Denervation; D009132:Muscles / Q000294:innervation*; D010243:Paralysis / Q000503:physiopathology; D011999:Recruitment, Neurophysiological / Q000502:physiology