title: Construction and characterization of partial digest DNA libraries made from flow-sorted human chromosome 16.
abstract: In this report, we present the techniques used for the construction of chromosome-specific partial digest libraries from flow-sorted chromosomes and the characterization of two such libraries from human chromosome 16. These libraries were constructed to provide materials for use in the development of a high-resolution physical map of human chromosome 16, and as part of a distributive effort on the National Laboratory Gene Library Project. Libraries with 20-fold coverage were made in Charon-40 (LA16NL03) and in sCos-1 (LA16NC02) after chromosome 16 was sorted from a mouse-human monochromosomal hybrid cell line containing a single homologue of human chromosome 16. Both libraries are approximately 90% enriched for human chromosome 16, have low nonrecombinant backgrounds, and are highly representative for human chromosome-16 sequences. The cosmid library in particular has provided a valuable resource for the isolation of coding sequences, and in the ongoing development of a physical map of human chromosome 16.
authors.affiliation: Life Sciences Division and Center for Human Genome Studies, Los Alamos National Laboratory, NM 87545.
authors.forename: J L
authors.initials: JL
authors.lastname: Longmire
authors.forename: N C
authors.initials: NC
authors.lastname: Brown
authors.forename: L J
authors.initials: LJ
authors.lastname: Meincke
authors.forename: M L
authors.initials: ML
authors.lastname: Campbell
authors.forename: K L
authors.initials: KL
authors.lastname: Albright
authors.forename: J J
authors.initials: JJ
authors.lastname: Fawcett
authors.forename: E W
authors.initials: EW
authors.lastname: Campbell
authors.forename: R K
authors.initials: RK
authors.lastname: Moyzis
authors.forename: C E
authors.initials: CE
authors.lastname: Hildebrand
authors.forename: G A
authors.initials: GA
authors.lastname: Evans
mesh_terms: D000818:Animals; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D005434:Flow Cytometry; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D010766:Phosphorylation
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases