title: The influence of doxycycline of the attachment of fibroblasts to gelatin-coated surfaces and its cytotoxicity.
abstract: Because of their antimicrobial and anti-inflammatory properties, tetracyclines have been used systemically and locally in the treatment of periodontal disease. This study was done to evaluate the influence of doxycycline (Dc), a tetracycline, on fibroblast adherence to a protein coated surface and its cytotoxicity. Periodontal ligament derived fibroblasts (PLDFs) were either: 1) preincubated with Dc (0 to 100 micrograms/ml) and then allowed to adhere to a gelatin-coated surface or 2) adherence was first established and then Dc added to the system. After an appropriate time the number of PLDFs adherent, released, or lysed was estimated by a lactic dehydrogenase (LDH) assay. Preincubation of PLDFs in 25 micrograms Dc/ml or higher concentrations significantly (P < 0.01) reduced the number of adherent cells. Fifty micrograms Dc/ml and higher doses significantly (P < 0.01) increased PLDFs cytotoxicity as measured by LDH release. The same trend was noted if PLDFs were allowed to adhere and then subjected to the drug. Microscopic observation of fluorescein diacetate/propidium iodide-stained cells showed that attached-spread cells pulled in, rounded up, and detached in proportion to the increased dose of Dc and the percentage of red-stained cytotoxic cells rose in a similar manner. The data suggested that Dc can be cytotoxic and may inhibit PLDFs adherence and spread along a substratum.
authors.affiliation: Department of Periodontology, School of Dentistry, Hokkaido University, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Tsukuda
authors.forename: W L
authors.initials: WL
authors.lastname: Gabler
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D004318:Doxycycline / Q000493:pharmacokinetics / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005780:Gelatin; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D005780:Gelatin; D007770:L-Lactate Dehydrogenase; D004318:Doxycycline