Restriction endonuclease analysis of Hungarian bovine herpesvirus isolates from different clinical forms of IBR, IPV and encephalitis.
The DNA of thirty-three Hungarian bovine herpesvirus isolates originating from cattle with various clinical symptoms were compared by restriction endonuclease analysis using HindIII, EcoRI and BstEII enzymes. The EcoRI and HindIII cleavage patterns were similar to those of isolates studied in other countries. Based on the cleavage patterns, the Hungarian isolates could be assorted into groups and subgroups according to the classification system proposed by Metzler et al. (1985, 1986). Based on a new BstEII cleavage pattern observed in group 1, the establishment of two new subgroups, 1a and 1b, were proposed. Three isolates belonged to subgroup 1a, sixteen to 1b, seven to 2a, five to 2b, and one to group 3, which has recently been reclassified as BHV-5. Additionally, one of the isolates showed a mixed cleavage pattern of 1a and 1b. However, no strict correlation was found between the different clinical forms and the established DNA fingerprint groups. There was no evidence of a change in the prevalence of the different genotypes when comparing isolates collected at different times of a 24-year period.
Magyar
G
G
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest.
Tanyi
J
J
Hornyák
A
A
Bartha
A
A
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000145:classification* / Q000235:genetics; D006814:Hungary; D015183:Restriction Mapping
D004279:DNA, Viral