Asialo-positive cells are overexpressed whereas IL-2 induced LAK activity is impaired in mice hyperimmunized with an immunomodulating mycobacterial fraction.
Previously reported studies revealed that extensive immunization with the Methanol Extraction Residue (MER) of BCG tubercle bacillus in Incomplete Freund's Adjuvant (IFA) induced marked suppression of T and B cell functions and that immunosuppression was correlated with marked decrease in splenic T-cell population and marked increase in the splenic macrophage population. The purpose of the present work was to determine if extensive immunization with MER in IFA also induced changes in splenic asialo antigen positive (NK) population and in the potential of induction of splenic LAK activity in vitro by recombinant IL-2. By comparison with other test groups, namely mice injected with IFA only, injected once with MER in aqueous suspension and normal, untreated mice, hyperimmunization with MER resulted in marked increase in expression of asialo positive (NK) splenic cells whereas induction of splenic LAK activity by IL-2 was markedly depressed. Splenic macrophages originated from MER hyperimmunized mice had no effect on induction of LAK activity by IL-2 in a normal splenic population.
Rashid
G
G
Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Israel.
Gittel
H
H
Ben-Efraim
S
S
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D015415:Biomarkers; D005260:Female; D007114:Immunization; D016130:Immunophenotyping; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000276:immunology; D013154:Spleen / Q000276:immunology
D001500:BCG Vaccine; D015415:Biomarkers; D007166:Immunosuppressive Agents; D007376:Interleukin-2