The antibody response of vitamin A-deficient rats to pneumococcal polysaccharide is enhanced through coimmunization with lipopolysaccharide.
Although the antibody response of vitamin A-deficient rats immunized with pneumococcal polysaccharide (SSS-III) was barely detectable, coimmunization with SSS-III plus 2 or 20 micrograms of Pseudomonas aeruginosa lipopolysaccharide (LPS) resulted in an anti-SSS-III response that was equal to or greater than that of vitamin A-sufficient rats immunized only with SSS-III. Doses of 2 and 20 micrograms of LPS, but not 0.1 and 0.5 micrograms, produced significant adjuvanticity, and LPS functioned as an adjuvant when given concomitantly with SSS-III (day 0) or up to 3 days later. Thus, vitamin A deficiency per se does not preclude the anti-SSS-III response, and LPS or factors released in response to LPS, even after initiation of the response to SSS-III, may substantially enhance antibody production to this thymus-independent, type 2 antigen.
Pasatiempo
A M
AM
Department of Biochemistry, Medical College of Pennsylvania, Philadelphia 19129.
Kinoshita
M
M
Foulke
D T
DT
Ross
A C
AC
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000921:Antibody-Producing Cells / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D005260:Female; D007115:Immunization Schedule; D007075:Immunoglobulin M / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013296:Streptococcus pneumoniae / Q000276:immunology*; D014802:Vitamin A Deficiency / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; C026604:pneumococcal polysaccharide, type III