title: Cloning and sequence analysis of cDNA for a human homolog of eubacterial ATP-dependent Lon proteases.
abstract: Overlapping cDNA clones containing mRNA for a putative Lon protease (LonHS) were isolated from cDNA libraries prepared from human brain poly(A)+ RNA. The determined nucleotide sequence contains a 2814-bp open reading frame with two potential initiation codons (positions 62-64 and 338-340). The 5'-terminal 337-nucleotide fragment of LonHS mRNA is highly enriched with G and C nucleotides and could direct synthesis of the LonHS N-terminal domain. More likely this region promotes initiation of protein synthesis from the second AUG codon in a cap-independent manner. The amino acid sequence initiated at the second AUG codon includes 845 residues, over 30% of which are identical to those of eubacterial Lon proteases. Residues of the 'A' and 'B' motifs of NTP-binding pattern and a plausible catalytic serine residue are conserved in LonHS. Northern blot analysis revealed LonHS mRNA in lung, duodenum, liver and heart, but not in thymus cells.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow.
authors.lastname: Amerik AYu
authors.forename: G V
authors.initials: GV
authors.lastname: Petukhova
authors.forename: V G
authors.initials: VG
authors.lastname: Grigorenko
authors.forename: I P
authors.initials: IP
authors.lastname: Lykov
authors.forename: S V
authors.initials: SV
authors.lastname: Yarovoi
authors.forename: V M
authors.initials: VM
authors.lastname: Lipkin
authors.forename: A E
authors.initials: AE
authors.lastname: Gorbalenya
mesh_terms: D049069:ATP-Dependent Proteases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D049070:Protease La; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; C483567:Lon protein, E coli; D049070:Protease La