title: Ileal distention relaxes the canine colon: a model of megacolon?
abstract: OBJECTIVE
  Reflex relaxation is a mechanism whereby the colon can show short-term dilatation in the absence of mechanical obstruction. This study investigated the tonic response of the canine colon to ileal distention and its pharmacological control.
  
  
  METHODS
  In four dogs, the tone of the proximal colon was recorded by a barostat during distention of the terminal ileum.
  
  
  RESULTS
  Ileal distention inhibited ileal motility and relaxed the colon. Adrenergic blockade by propranolol plus phentolamine, nicotinic blockade by hexamethonium, and the nitric oxide synthase inhibitor N omega-nitro-L-arginine methylester significantly increased resting tone of the colon but did not inhibit relaxation induced by ileal distention. Muscarinic blockade by atropine completely relaxed the colon, and no further decrease in tone was observed after ileal distention. The neurokinin 2 antagonist SR48968 did not alter colonic tone.
  
  
  CONCLUSIONS
  The barostat was able to monitor resting tone of the canine colon, which was shown to be under inhibitory control by adrenergic, cholinergic-nicotinic, and nitric oxide-like transmitters. Inhibition of colonic tone by ileal distention was not mediated solely by adrenergic, cholinergic-nicotinic, or nitric oxide mechanisms. Reflex relaxation, possibly predisposing to acute colonic dilatation, may be activated by multiple mechanisms that may differ from those controlling resting tone.
authors.affiliation: Gastroenterology Research Unit, Mayo Clinic, Rochester, Minnesota.
authors.forename: G
authors.initials: G
authors.lastname: Basilisco
authors.forename: S F
authors.initials: SF
authors.lastname: Phillips
mesh_terms: D000818:Animals; D003106:Colon / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005769:Gastrointestinal Motility; D007082:Ileum / Q000502:physiology*; D008531:Megacolon / Q000503:physiopathology*; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*