title: Thy-1 antigen dendritic cells and rejection of composite tissue limb allografts in rats.
abstract: Dendritic-shaped cells (Thy-1+DC) characterized by the surface phenotype of Thy-1+, asialo GM1+, Ia- are reported to exist exclusively in mice. However, Thy-1+ dendritic cells were observed in the skin of normal athymic nude F344, euthymic F344, and WKAH rats. Their phenotypic markers were Thy-1+, asialo GM1+, Ia-, W3/25 (helper/inducer)-, OX8 (cytotoxic/suppressor)-, W3/13 (pan T lymphocytes)-, OX39 (IL-2)-, and OX41 (macrophages)-. These phenotypic markers were analogous to those of mice. The density of Thy-1+DC in the skin of normal nude F344 rats was approximately twice as much as that of F344 and WKAH rats. When WKAH rats were used as donors with nude F344 rats as recipients (this combination being a major mismatch with regard to a MHC, rejection-like changes such as edema and erythema were observed 1 week after the grafting. An immunohistochemical examination demonstrated that the density of Thy-1+DC had increased 3-fold within the donor skin at 1 week after transplantation, although almost no W3/25 positive cells, OX8 positive cells, or other inflammatory cells were observed. Within 2 weeks of transplantation the donor skin ceased rejection-like changes and the density of Thy-1+DC was reduced to preoperation levels. When WKAH rats were used as donors with F344 rats as recipients, severe rejection was observed, and many T cells with W3/25 or OX8 antigens and granulocytes had infiltrated into the skin at the donor sites. Thy-1+DC could not be found in the donor skin. These findings suggest that a correlation exists between Thy-1+DC and rejection-like changes in the donor skin when nude rats are used as recipients.
authors.affiliation: Department of Orthopaedic Surgery, Nagoya University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ueda
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: N
authors.initials: N
authors.lastname: Ishiguro
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
authors.forename: J
authors.initials: J
authors.lastname: Asai
authors.forename: T
authors.initials: T
authors.lastname: Miura
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D003713:Dendritic Cells / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D006614:Hindlimb / Q000637:transplantation*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D011923:Rats, Nude; D016038:Skin Transplantation / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D018800:Thy-1 Antigens; D014184:Transplantation, Homologous / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D018800:Thy-1 Antigens