title: Clinical significance of antibodies to native or denatured 60-kd or 52-kd Ro/SS-A proteins in Sjögren's syndrome.
abstract: OBJECTIVE
  To evaluate the clinical significance of antibodies to native or denatured (anti-n or anti-d) 60- or 52-kd Ro/SS-A proteins (60K or 52K) in Sjögren's syndrome (SS).
  
  
  METHODS
  The presence of antibodies to denatured and native Ro/SS-A proteins was determined by immunoblotting and immunoprecipitation, respectively. Salivary gland dysfunction was evaluated by salivary function scintigraphy.
  
  
  RESULTS
  The incidence of anti-d-60K without anti-d-52K was lower among patients with systemic lupus erythematosus with SS (SLE/SS) and among those with primary SS, compared with patients who had SLE without SS, whereas anti-d-52K without anti-d-60K was more common in SLE/SS patients and primary SS patients than in SLE patients without SS. All of the patients with anti-Ro/SS-A had anti-n-60K. Serologic abnormalities and salivary gland dysfunction were associated with anti-n-60K in SS, whereas Hashimoto's thyroiditis in SS was related to anti-d-60K. Anti-d-52K was not associated with any extraglandular or glandular symptoms in SS.
  
  
  CONCLUSIONS
  The data indicate that anti-n-60K, which appears to recognize conformational epitopes, is associated with clinical features of SS characterized by glandular dysfunction.
authors.forename: K
authors.initials: K
authors.lastname: Tsuzaka
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.forename: M
authors.initials: M
authors.lastname: Akizuki
authors.forename: T
authors.initials: T
authors.lastname: Mimori
authors.forename: T
authors.initials: T
authors.lastname: Tojo
authors.forename: H
authors.initials: H
authors.lastname: Fujii
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukatani
authors.forename: A
authors.initials: A
authors.lastname: Kubo
authors.forename: M
authors.initials: M
authors.lastname: Homma
mesh_terms: D001323:Autoantibodies / Q000032:analysis / Q000737:chemistry*; D001324:Autoantigens / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D011233:Precipitin Tests; D011489:Protein Denaturation; D020733:RNA, Small Cytoplasmic; D011877:Radionuclide Imaging; D012261:Ribonucleoproteins / Q000097:blood / Q000276:immunology*; D012469:Salivary Glands / Q000737:chemistry / Q000000981:diagnostic imaging; D012859:Sjogren's Syndrome / Q000097:blood / Q000276:immunology*; D013670:Sodium Pertechnetate Tc 99m; D000099207:SS-A Antigen
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D013670:Sodium Pertechnetate Tc 99m; D000099207:SS-A Antigen; C116006:RO60 protein, human