title: Analysis of FCE 23762 (methoxymorpholinodoxorubicin hydrochloride), a new antitumour agent, by HPTLC and scanning densitometry.
abstract: A simple, rapid and reproducible high-performance thin-layer chromatographic (HPTLC) method using UV or fluorescence scanning densitometry has been developed for the assay and purity control of methoxymorpholinodoxorubicin hydrochloride (FCE 23762). With a mobile phase of chloroform-methanol-acetic acid (93:6:1, v/v/v) and a silica gel plate, all potential impurities were separated from the main component and from each other. Detection limits at a signal-to-noise ratio of 2:1 were a few nanograms for UV detection and < 1 ng for fluorescence emission. The RSD values for the examined compounds were all < 3%.
authors.affiliation: Istituto Superiore di Sanità, Roma, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Farina
authors.forename: M G
authors.initials: MG
authors.lastname: Quaglia
authors.forename: A
authors.initials: A
authors.lastname: Doldo
authors.forename: S
authors.initials: S
authors.lastname: Calandra
authors.forename: F R
authors.initials: FR
authors.lastname: Gallo
mesh_terms: D000085:Acetates; D019342:Acetic Acid; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid; D003720:Densitometry; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000032:analysis; D000432:Methanol; D012015:Reference Standards
chemical_list: D000085:Acetates; C066998:methoxy-morpholinyl-doxorubicin; D002725:Chloroform; D004317:Doxorubicin; D019342:Acetic Acid; D000432:Methanol