title: Site-specific recombinases: tools for genome engineering.
abstract: Site-specific recombinases from bacteriophage and yeasts have been developed as novel tools for manipulating DNA both in the test-tube and in living organisms. We discuss the characteristics of these enzyme systems, review their application in genetic and developmental studies and speculate on their future potential for large-scale directed modifications of eukaryotic genomes.
authors.affiliation: Institute of Biotechnology, University of Cambridge, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Kilby
authors.forename: M R
authors.initials: MR
authors.lastname: Snaith
authors.forename: J A
authors.initials: JA
authors.lastname: Murray
mesh_terms: D000818:Animals; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D005818:Genetic Engineering; D016678:Genome; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: D004254:DNA Nucleotidyltransferases