[Production and inhibition of eosinophil colony stimulating factor by lymphocytes from patients with bronchial asthma].
To clarify the mechanism of the eosinophilia in patients with bronchial asthma (BA), we examined the production and inhibition of eosinophil colony stimulating factor (Eo-CSF) of mononuclear cells (MNC) and lymphocytes from BA. After bone marrow cells from normal volunteers (NA) were incubated with culture media of MNC or lymphocytes cultured in the presence of interleukin-2 (IL-2, 1 U/ml) or Dermatophagoides farinae (10 micrograms/ml, Df), the number of eosinophils was increased and GM-CSF production was increased after 5 days of culture. Interleukin 5 (IL-5) production was increased in some cases of BA, but did not differ between BA and NA delete. When MNC were incubated in the presence of IL-2 and interleukin 4 (IL-4), neither eosinophilia nor GM-CSF production occurred. These results suggest first that MNC and T cells in BA have increased responsiveness to IL-2 and Df, second that increased GM-CSF production partly causes eosinophilia, and third that IL-4 inhibited IL-2-induced eosinophil proliferation by inhibition of GM-CSF production.
Tanida
K
K
Third Department of Internal Medicine, School of Medicine, Tokushima University, Japan.
Nakamura
Y
Y
Kamei
T
T
Ozaki
T
T
Ogura
T
T
D000328:Adult; D000818:Animals; D001249:Asthma / Q000150:complications*; D002455:Cell Division; D002478:Cells, Cultured; D004804:Eosinophils / Q000166:cytology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D015848:Interleukin-5 / Q000096:biosynthesis; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D011657:Pulmonary Eosinophilia / Q000209:etiology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D015848:Interleukin-5; D008222:Lymphokines; C022227:eosinophil stimulating promoter; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor