title: A method for detection of aromatic metabolites at very low concentrations: application to detection of metabolites of anaerobic toluene degradation.
abstract: Difficulties inherent in working with anaerobic microorganisms and mixed cultures have hampered efforts to detect and identify metabolites of anaerobic degradation of monoaromatic compounds. Isotope-trapping experiments and analysis using a high-performance liquid chromatograph equipped with a flow-through radioactivity detector were used to detect very low concentrations of metabolites. Data obtained by this method suggest that toluene was degraded via methyl hydroxylation by a mixed methanogenic culture.
authors.affiliation: Department of Civil Engineering, Standford University, California 94305-4020.
authors.forename: E A
authors.initials: EA
authors.lastname: Edwards
authors.forename: A M
authors.initials: AM
authors.lastname: Edwards
authors.forename: D
authors.initials: D
authors.lastname: Grbić-Galić
mesh_terms: D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000706:statistics & numerical data; D019605:Euryarchaeota / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D006900:Hydroxylation; D012680:Sensitivity and Specificity; D014050:Toluene / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D014050:Toluene