Adoptive transfer of suppressor T cells induced by Actinobacillus actinomycetemcomitans regulates immune response.
Purified splenic T cells from C3H/HeN mice primed with immunosuppressive fraction (ISF) from A. actinomycetemcomitans were adoptively transferred to syngeneic mice with SRBC. The transfer of splenic T cells from mice, primed with various amounts of ISF for 6 days, resulted in the dose-dependent inhibition of IgM anti-sheep red blood cells (SRBC) plaque-forming cells (PFC) compared with normal and BSA-primed splenic T cells. Furthermore, the transfer of T cells from mice primed with 100 micrograms of ISF for 6 days to syngeneic and CD4-depleted mice caused the highest inhibition. Immune suppression did not depend on the B cell population in spleen from donor mice primed with ISF, nor on haplotypes as recipient. The immunosuppressive function of these ISF-primed T cells was abrogated by 1000 rad irradiation. Splenic T cells from ISF-treated mice could suppress the T cell-dependent proliferative responses of cocultured normal spleen cells in vitro. Analysis of T cell subsets of spleen cells from ISF-treated mice showed that the suppressor cell is susceptible to treatment with anti-CD8 and complement (C). ISF-sensitized suppressor T cells also suppressed secondary IgG anti-SRBC-PFC response after immunization with SRBC in vivo depending on sensitized periods induced by ISF. Treatment of T cells from mice which primed with ISF for 8 days, with goat anti-mouse CD8 antibody and C abrogated their suppressive effects, and secondary IgG response occurred. These results indicate that the adoptive transfer of ISF-induced T cells, which increased suppressor function, caused the perfect blocking of immune response, allowing promotion of secondary infection.
Kurita-Ochiai
T
T
Department of Microbiology, Nihon University School of Dentistry, Matsudo, Japan.
Ochiai
K
K
Saito
N
N
Ikeda
T
T
D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001685:Biological Factors / Q000494:pharmacology; D016827:CD8 Antigens / Q000276:immunology; D005260:Female; D006462:Hemolytic Plaque Technique; D007165:Immunosuppression Therapy / Q000379:methods*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010518:Periodontitis / Q000382:microbiology; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000637:transplantation*
D000907:Antibodies, Bacterial; D001685:Biological Factors; D016827:CD8 Antigens; D007166:Immunosuppressive Agents