High-performance liquid chromatographic determination of m-iodobenzylguanidine in urine of cancer patients.
An accurate, sensitive, reproducible and selective HPLC assay is presented for the quantitative determination of m-iodobenzylguanidine (MIBG) in human urine. The sample pretreatment involves a solid-phase extraction on Bakerbond SPE cyano columns. The HPLC systems consists of a muBondapak C18 column and 25 mM ammonium phosphate (pH 3.0)-acetonitrile (75:25, v/v) as the mobile phase. Detection is performed by UV absorbance at 254 nm. Log y vs. log x is the response function that yielded the smallest sum of percent relative concentration residuals over the whole concentration range of the assay (0.2-100 micrograms/ml). The excretion profile of MIBG in urine of a three-year old patient is shown.
Wafelman
A R
AR
Department of Pharmacy, Slotervaart Hospital, Amsterdam, Netherlands.
Nortier
Y L
YL
Rosing
H
H
Underberg
W J
WJ
Maes
R A
RA
Beijnen
J H
JH
D019797:3-Iodobenzylguanidine; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000652:urine*; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007457:Iodine Radioisotopes; D007462:Iodobenzenes / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000652:urine*; D008297:Male; D009369:Neoplasms / Q000188:drug therapy / Q000652:urine*; D009447:Neuroblastoma / Q000188:drug therapy / Q000652:urine; D013056:Spectrophotometry, Ultraviolet
D000970:Antineoplastic Agents; D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D019797:3-Iodobenzylguanidine