IL-5 in post-traumatic eosinophilic pleural effusion.
Thoracic trauma or pneumothorax can result in pleural fluid eosinophilia. In this study we investigated the role of the eosinophilopoietic cytokine IL-5 in three cases of post-traumatic eosinophilic pleural effusions (EPE). Using a specific immunoenzymatic assay, significant levels of IL-5 were found in EPE (range 100-3000 pg/ml), while IL-5 was undetectable (< 25 pg/ml) in corresponding serum samples and in non-eosinophilic pleural fluids. IL-5 present in pleural fluids was found bioactive in a proliferative assay using a mouse CTLL-2 cell line transfected with the cDNA corresponding to the alpha chain of the human IL-5 receptor. Using a reverse polymerase chain reaction (PCR) method, we found IL-5 mRNA expression within pleural mononuclear cells from patients with EPE, but not in corresponding peripheral blood mononuclear cells (PBMC), confirming that IL-5 is synthesized locally in the pleural cavity. In the two cases in which pleural CD4+ cells were purified, these cells were identified as the major source of IL-5. Taken together, these data indicate that the development of post-traumatic EPE is related to a local secretion of IL-5 by CD4+ cells present in the pleural cavity.
Schandené
L
L
Department of Immunology-Haematology, Hôpital Erasme, Université Libre de Bruxelles, Belgium.
Namias
B
B
Crusiaux
A
A
Lybin
M
M
Devos
R
R
Velu
T
T
Capel
P
P
Bellens
R
R
Goldman
M
M
D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D005260:Female; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D015848:Interleukin-5 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010996:Pleural Effusion / Q000209:etiology / Q000276:immunology*; D011030:Pneumothorax / Q000150:complications; D016133:Polymerase Chain Reaction; D011657:Pulmonary Eosinophilia / Q000209:etiology / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D013898:Thoracic Injuries / Q000150:complications
D015848:Interleukin-5; D012333:RNA, Messenger