Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus.
A continuous cell-free protein synthesis system of an extremely thermophilic eubacterium, Thermus thermophilus HB27, was constructed. This system produced MS2 phage RNA translation products at a rate of more than 5 micrograms per hour per 1.9 mg of ribosomes at 65 degrees C and the production continued linearly for at least 340 min. When no polyamine was added, the system did not produce the proteins. The highest activity was recorded when 0.1 mM tetrakis(3-aminopropyl)ammonium and 1.0 mM spermine were added simultaneously.
Uzawa
T
T
Department of Life Science, Tokyo Institute of Technology, Yokohama.
Yamagishi
A
A
Ueda
T
T
Chikazumi
N
N
Watanabe
K
K
Oshima
T
T
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002474:Cell-Free System; D003470:Culture Media; D014176:Protein Biosynthesis; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes; D013096:Spermine / Q000494:pharmacology*; D016963:Thermus thermophilus / Q000187:drug effects / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D012333:RNA, Messenger; C053493:tetrakis(3-aminopropyl)ammonium; D013096:Spermine