Molecular basis of the ribulose-1,5-bisphosphate carboxylase/oxygenase activase mutation in Arabidopsis thaliana is a guanine-to-adenine transition at the 5'-splice junction of intron 3.
Analysis of the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase gene and gene products from Arabidopsis thaliana wild-type plants and the Rubisco activase-deficient mutant strain showed that the rca mutation caused GT to be changed to AT at the 5'-splice junction of intron 3 in the six-intron pre-mRNA. Northern blot analysis, genomic and cDNA sequencing, and primer extension analysis indicated that the mutation causes inefficient and incomplete splicing of the pre-mRNA, resulting in the accumulation of three aberrant mRNAs. One mutant mRNA was identical with wild-type mRNA except that it included intron 3, a second mRNA comprised intron 3 and exons 4 through 7, and the third mRNA contained exons 1 through 3. The G-to-A transition is consistent with the known mechanism of mutagenesis by ethyl methanesulfonate, the mutagen used to create the Rubisco activase-deficient strain.
Orozco
B M
BM
Department of Plant Biology, University of Illinois at Urbana-Champaign.
McClung
C R
CR
Werneke
J M
JM
Ogren
W L
WL
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D007438:Introns; D008969:Molecular Sequence Data; D010641:Phenotype; D010940:Plant Proteins; D017354:Point Mutation; D012326:RNA Splicing / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; C054765:rca protein, plant; D012273:Ribulose-Bisphosphate Carboxylase