Bloom's syndrome cells GM1492 lack detectable p53 protein but exhibit normal G1 cell-cycle arrest after UV irradiation.
The tumor suppressor gene p53 is thought to be a key factor in the onset of G1 cell-cycle arrest following DNA damage. However, here we describe cells derived from a patient with Bloom's syndrome, lacking any detectable p53 protein, that still shows a functional G1 cell-cycle checkpoint after irradiation with UV-C. Comparison with cells from other Bloom's patients showed that the absence of p53 protein is not a specific characteristic of Bloom's syndrome.
van Laar
T
T
Laboratory of Molecular Carcinogenesis, Sylvius Laboratories, Leiden University, The Netherlands.
Steegenga
W T
WT
Jochemsen
A G
AG
Terleth
C
C
van der Eb
A J
AJ
D001816:Bloom Syndrome / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D016193:G1 Phase / Q000528:radiation effects; D006801:Humans; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000378:metabolism*; D014466:Ultraviolet Rays
D016159:Tumor Suppressor Protein p53