[Prolonged psychoemotional stress as an inducer of mutations in mammals and as a modifier of mutagenesis].
The present study was made to determine the genotoxicity of psychoemotional stress (PES) alone and its possible influence on chemical mutagenesis under combined treatment. Stress was registered by the classical parameters: 1) weights of the thymus and adrenal; 2) stomach and duodenal mucosa conditions. The unscheduled DNA synthesis (UDS) measurement in peripheral blood lymphocytes with simultaneous determination of chromosomal aberrations (CA) and micronuclei (MN) levels in bone marrow cells were applied for estimation the mutagenic activity. The results indicated that PES alone, as CP, induced UDS in peripheral blood lymphocytes. Intensity of UDS was dependent on the stress duration. The UDS kinetics in the lymphocytes from stressed mice and stressed mice treated with CP were different. PES and CP induced the similar amounts of CA in bone marrow cells. Value of CA had a comparable tendency with results of UDS. The combined effect of PES and CP in both tests was different from additive one. The effects in NM test were statistically significant only in case of combined PES and CP action. The mutagenic potency of PES and its role in the modification of environmental mutagenesis will be discussed.
Ingel'
F I
FI
Gevorkian
N M
NM
Iliushina
N A
NA
Leĭtina
B I
BI
Prikhozhan
L M
LM
Pereverzeva
E L
EL
Revazova
Iu A
IuA
D000818:Animals; D002908:Chronic Disease; D003520:Cyclophosphamide / Q000633:toxicity; D005796:Genes / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D016296:Mutagenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D013315:Stress, Psychological / Q000235:genetics*; D013997:Time Factors
D003520:Cyclophosphamide
Dlitel'nyĭ psikhoémotsional'nyĭ stress kak induktor mutatsiĭ u mlekopitaiushchikh i modifikator mutageneza.