Coamplification on chromosomes 7p12-13 and 9q12-13 identified by reverse chromosome painting in a glioblastoma multiforme.
DNA amplification is known to occur in approximately 50% of glioblastomas, with the epidermal growth factor receptor (EGFR) gene being the most frequently amplified. Whereas previous amplification studies have largely been limited to the analysis of known tumor-related genes, reverse chromosome painting allows us to search for as yet unidentified amplified domains. Here, we report the analysis of a glioblastoma multiforme by reverse chromosome painting. Hybridization signals were found on chromosome 7p12-13 and chromosome 9q12-13. Standard Southern blot analysis revealed amplification of the EGFR gene, which is localized on band 7p13. These findings corroborate previous reports on coamplification of sequences on different chromosomes in glioblastoma.
Fischer
U
U
Institut für Humangenetik, Universität des Saarlandes, Homburg, Germany.
Wullich
B
B
Sattler
H P
HP
Göttert
E
E
Zang
K D
KD
Meese
E
E
D000368:Aged; D015139:Blotting, Southern; D002897:Chromosomes, Human, Pair 7; D002899:Chromosomes, Human, Pair 9; D066246:ErbB Receptors / Q000235:genetics*; D005784:Gene Amplification; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D014407:Tumor Cells, Cultured
D066246:ErbB Receptors