Identification of feline- and canine-like rotaviruses isolated from humans by restriction fragment length polymorphism assay.
Restriction fragment length polymorphism assay of reverse-transcribed and polymerase chain reaction-amplified rotavirus gene segment 9 was developed to differentiate human serotype 3 rotaviruses from animal serotype 3 rotaviruses. On the basis of similarities or differences in HinfI and DdeI restriction profiles, unusual group A serotype 3 human rotaviruses that belonged to subgroup I were shown to be of feline and canine origin. By this approach, the new human rotavirus isolates 5193, AU-387, AU-720, AU-785 and AU-1115 were shown to resemble certain feline-like human rotaviruses. Similar results were previously obtained by Nakagomi et al. (O. Nakagomi, A. Hoshima, Y. Aboudy, I. Shif, M. Mochizuki, T. Nakagomi, and T. Gotlieb-Stematsky. J. Clin. Microbiol. 28:1198-1203, 1990) by using RNA-RNA cross hybridization with established feline rotaviruses. The restriction fragment length polymorphism assay can provide fast and valuable information on the interspecies transmission of rotaviruses in nature.
Vonsover
A
A
Central Virology Laboratory, Chain Sheba Medical Center, Tel-Hashomer, Israel.
Shif
I
I
Silberstein
I
I
Rudich
H
H
Aboudy
Y
Y
Mendelson
E
E
Shulman
L
L
Nakagomi
T
T
Nakagomi
O
O
D000818:Animals; D002415:Cats / Q000382:microbiology; D004285:Dogs / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology; D016679:Genome, Viral; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000382:microbiology / Q000635:transmission; D012703:Serotyping; D013045:Species Specificity