Trained and amphetamine-induced circling behavior in lesioned, transplanted rats.
Rats were trained to turn for water reinforcement and then were given unilateral 6-hydroxydopamine lesions. After lesion, rats showed deficits in trained turning both contra- and ipsilateral to the side of the lesion, with contralateral turning more severely impaired. The lesioned rats were then transplanted with fetal mesencephalic dopamine tissue into striatum. A control group of lesioned rats were sham transplanted. Four weeks after transplant, 1.5 mg/kg D-amphetamine challenge injections were used to test the functioning of the transplants. In the control rats, D-amphetamine induced ipsilateral turning; in transplanted rats, D-amphetamine slowed the rate of ipsilateral turning or reversed the direction of amphetamine-induced rotation. Only rats which reversed their amphetamine-induced turn direction after transplant were used for the rest of the experiment. Trained turning was assessed at 4, 8, 12 and 16 weeks post transplant. Transplants did not improve learned performance at any time post transplant. When D-amphetamine was administered in conjunction with the trained turning sessions, a low dose (0.12 mg/kg) enhanced contralateral trained turn rates, without affecting ipsilateral turn rates. Higher doses of amphetamine reduced ipsilateral turn rate in the transplanted animals. The results of this study suggest that transplants alone do not reinstate performance of conditioned rotation.
Richards
J B
JB
Division of Clinical Pharmacology and Toxicology, University of Colorado Health Sciences Center, Denver 80262.
Sabol
K E
KE
Kriek
E H
EH
Freed
C R
CR
D000818:Animals; D016380:Brain Tissue Transplantation; D003216:Conditioning, Operant / Q000502:physiology*; D003342:Corpus Striatum / Q000187:drug effects / Q000503:physiopathology; D003913:Dextroamphetamine / Q000494:pharmacology*; D004298:Dopamine / Q000502:physiology*; D016332:Fetal Tissue Transplantation; D006085:Graft Survival; D008297:Male; D008569:Memory Disorders / Q000503:physiopathology*; D008636:Mesencephalon / Q000196:embryology / Q000637:transplantation*; D009043:Motor Activity / Q000187:drug effects*; D016627:Oxidopamine / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley
D016627:Oxidopamine; D003913:Dextroamphetamine; D004298:Dopamine