Nucleotide sequence and genetic organization of a 7.3 kb region (map unit 47 to 52.5) of Autographa californica nuclear polyhedrosis virus fragment EcoRI-C.
The nucleotide sequence and genetic organization of a 7297 bp region within the EcoRI-C fragment of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV) are presented. Eight putative open reading frames were found and their respective amino acid sequences compared with a number of data libraries. ORF 1227 corresponded with gp41 and its predicted protein sequence was found to be 55 amino acids longer at its C terminus than reported previously. Moreover the main part of the ORF 1227 product, including the additional 55 amino acids, showed a high degree of homology with protein p40 of Helicoverpa zea single nucleocapsid nuclear polyhedrosis virus (HzSNPV). Three other ORFs in the analysed AcMNPV region showed homology with ORFs in the HzSNPV sequence, indicating that the general organization of this region is similar in both viruses. However one ORF found in the AcMNPV sequence was absent from the corresponding HzSNPV sequence.
Kool
M
M
Department of Virology, Wageningen Agricultural University, The Netherlands.
Broer
R
R
Zuidema
D
D
Goldbach
R W
RW
Vlak
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015246:Deoxyribonuclease EcoRI; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics
D015678:Viral Structural Proteins; D015246:Deoxyribonuclease EcoRI