title: Cyclothialidine, a novel DNA gyrase inhibitor. II. Isolation, characterization and structure elucidation.
abstract: Cyclothialidine is a novel DNA gyrase inhibitor produced by Streptomyces filipinensis NR 0484. It was isolated from the culture broth by charcoal adsorption, Diaion HP-21, Amberlite CG-50, DEAE Toyopearl, and Toyopearl HW-40 SF column chromatography. The structure of cyclothialidine was determined to be a unique twelve membered lactone by amino acid analysis and various 2D-NMR experiments. Cyclothialidine inhibited Escherichia coli DNA gyrase with an IC50 of 30 ng/ml.
authors.affiliation: Nippon Roche Research Center, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kamiyama
authors.forename: N
authors.initials: N
authors.lastname: Shimma
authors.forename: T
authors.initials: T
authors.lastname: Ohtsuka
authors.forename: N
authors.initials: N
authors.lastname: Nakayama
authors.forename: Y
authors.initials: Y
authors.lastname: Itezono
authors.forename: N
authors.initials: N
authors.lastname: Nakada
authors.forename: J
authors.initials: J
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Yokose
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli; D006571:Heterocyclic Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic; D011758:Pyrroles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013302:Streptomyces / Q000378:metabolism*; D059005:Topoisomerase II Inhibitors
chemical_list: D000596:Amino Acids; D006571:Heterocyclic Compounds; D010456:Peptides, Cyclic; D011758:Pyrroles; D059005:Topoisomerase II Inhibitors; C085889:cyclothialidine