Antiproliferative effects of heparin on normal and transformed NIH/3T3 fibroblasts.
We studied the effect of heparin on proliferation and signalling in normal NIH/3T3 fibroblasts, and in cells transformed by different oncogenes. Heparin inhibited the proliferation of normal as well as of v-sis and v-erbB transformed fibroblasts in the presence of serum, but failed to inhibit v-erbB-driven proliferation in serum-starved cultures; under these conditions, heparin inhibited by approximately 50% the proliferation of normal and v-sis- transformed cells. Heparin also inhibited PDGF-induced cell proliferation and inositol lipid turnover in v-sis transformants, but it did not affect PDGF mitogenic signalling in NIH/3T3 fibroblasts.
Cavari
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Institute of General Pathology, University of Firenze, Italy.
Ruggiero
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Vannucchi
S
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D016475:3T3 Cells / Q000187:drug effects*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D006131:Growth Inhibitors / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D051379:Mice; D018739:Oncogene Proteins v-erbB; D020575:Oncogene Proteins v-sis; D009857:Oncogenes; D010716:Phosphatidylinositols / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology; D015682:Retroviridae Proteins, Oncogenic / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects
D006131:Growth Inhibitors; D018739:Oncogene Proteins v-erbB; D020575:Oncogene Proteins v-sis; D010716:Phosphatidylinositols; D010982:Platelet-Derived Growth Factor; D015682:Retroviridae Proteins, Oncogenic; D006493:Heparin