title: Proteolysis of human native and oxidised alpha 1-proteinase inhibitor by matrilysin and stromelysin.
abstract: Matrilysin is shown to rapidly inactivate alpha 1PI, an inhibitor of elastase, by cleaving the Pro357-Met358 peptide bond of its reactive centre. The rate of inactivation of alpha 1PI by matrilysin is four times higher than stromelysin. Matrilysin cleaves oxidised alpha 1PI at the Phe352-Leu353 bond, whilst stromelysin cleaves oxidised alpha 1PI at the Met358-Ser359 bond. We conclude that matrilysin is a potent serpinase which could play a role in inflammatory tissue damage by proteolytically inactivating alpha 1PI.
authors.affiliation: Inflammation Research Group, London Hospital Medical College, University of London, UK.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: P G
authors.initials: PG
authors.lastname: Winyard
authors.forename: K
authors.initials: K
authors.lastname: Chidwick
authors.forename: G
authors.initials: G
authors.lastname: Murphy
authors.forename: M
authors.initials: M
authors.lastname: Wardell
authors.forename: R W
authors.initials: RW
authors.lastname: Carrell
authors.forename: D R
authors.initials: DR
authors.lastname: Blake
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D019278:Matrix Metalloproteinase 3; D020783:Matrix Metalloproteinase 7; D008666:Metalloendopeptidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D000515:alpha 1-Antitrypsin / Q000378:metabolism*
chemical_list: D000515:alpha 1-Antitrypsin; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D020783:Matrix Metalloproteinase 7