A 29,000 molecular weight fraction from fetal rat liver adhering cells that cooperates with erythropoietin in stimulating the growth of erythroid progenitors.
The erythroid-potentiating effects of a protein fraction produced by 20-day rat fetal liver-adhering cells are studied. Partial purification by gel filtration gave an active fraction (apparent molecular weight = 29 x 10(3)) that significantly increased the erythroid colony counts (CFUe and late BFUe) in cultures of liver cell fractions depleted of adhering cells at both limiting and saturating concentration of recombinant human erythropoietin. The sensitivity of CFUe and BFUe to erythropoietin was increased by the activator.
Nagel
M D
MD
ERS CNRS F0017, Laboratoire de Physiologie Animale, UFR Sciences Exactes et Naturelles, Reims, France.
D000818:Animals; D002448:Cell Adhesion; D002457:Cell Extracts / Q000502:physiology; D002850:Chromatography, Gel; D017077:Culture Media, Conditioned; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000187:drug effects; D004920:Erythropoiesis / Q000502:physiology*; D004921:Erythropoietin / Q000502:physiology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D008970:Molecular Weight; D011247:Pregnancy; D051381:Rats
D002457:Cell Extracts; D017077:Culture Media, Conditioned; D004921:Erythropoietin