[Analysis of susceptibility genes in sarcoidosis].
In order to investigate immunogenetic mechanisms in the pathogenesis of sarcoidosis, sixty-three patients with sarcoidosis, who were diagnosed histologically, were tested for HLA antigens. Controls consisted of 120 healthy subjects. A lymphocyte cytotoxicity test was used for typing HLA-A, -B, -C, -DQ and -DR antigens. HLA-DP genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The frequencies of HLA-DR52 and its associated antigens (HLA-DR5, -DR6 and -DR8) were significantly increased in the patients as compared to the control subjects. On the contrary, the frequencies of HLA-DR53, -DR1, and -DR4 were significantly decreased in the patients. The frequency of HLA-DPB1*0402 was significantly lower in the patients. No significant differences were observed in HLA-DQ antigens. These results suggest that HLA-DR52 associated antigens contribute to the susceptibility to sarcoidosis in Japanese.
Ishihara
M
M
Department of Ophthalmology, Yokohama City University School of Medicine, Kanagawa-ken, Japan.
Ohno
S
S
Ishida
T
T
Kagiya
M
M
Ando
H
H
Inoko
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D004198:Disease Susceptibility; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D006682:HLA-DP Antigens / Q000235:genetics; D059868:HLA-DP beta-Chains; D006801:Humans; D008285:Major Histocompatibility Complex; D008297:Male; D008875:Middle Aged; D012507:Sarcoidosis / Q000235:genetics*
D006680:HLA Antigens; D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen