title: Functional analysis of the mouse homeobox gene HoxB9 in Drosophila development.
abstract: Mammalian genomes contain clusters of homeobox genes (Hox-C, HOX-C) which are structurally similar to the homeotic genes of the Drosophila HOM complex. One method for assessing the functional similarity of particular Drosophila HOM and mammalian Hox genes is to test the ability of Hox genes to induce homeotic phenotypes when expressed in developing Drosophila. Here we describe such functional tests using mouse HoxB9 (formerly Hox-2.5), whose closest structural relative in Drosophila is Abdominal-B. When expressed from a heat shock promoter, HoxB9 induces transformations of head towards more posterior identities in Drosophila larvae and adults. These transformations share some similarities with the phenotypic effects produced by ectopically expressed Abdominal-B, but are also similar to the transformations induced by Antennapedia and mouse HoxB6 (Hox-2.2), suggesting that HoxB9 specifies a positional identity that is intermediate between Antennapedia and Abdominal-B.
authors.affiliation: Department of Genetics, Yale University, New Haven, CT 06511.
authors.forename: J
authors.initials: J
authors.lastname: Malicki
authors.forename: L D
authors.initials: LD
authors.lastname: Bogarad
authors.forename: M M
authors.initials: MM
authors.lastname: Martin
authors.forename: F H
authors.initials: FH
authors.lastname: Ruddle
authors.forename: W
authors.initials: W
authors.lastname: McGinnis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D005801:Genes, Homeobox; D006360:Heat-Shock Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000737:chemistry; D007814:Larva / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010641:Phenotype; D011506:Proteins; D014170:Transformation, Genetic
chemical_list: D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D018398:Homeodomain Proteins; C104161:Hoxb9 protein, mouse; D011506:Proteins