Vasculotropin/vascular endothelial growth factor induces differentiation in cultured osteoblasts.
Vasculotropin/vascular endothelial growth factor (VAS/VEGF) is an angiogenic growth factor whose biological activity seems to be restricted in vitro to vascular endothelial cells. We describe here that fetal bovine osteoblasts (OB) bind VAS/VEGF but do not proliferate upon its addition. However VAS/VEGF induces migration, PTH-dependent cAMP accumulation and alkaline phosphatase increase when added to OB. The maximal effects reach levels comparable to that obtained with bone morphogenetic protein 2 (BMP-2), although the VAS/VEGF concentrations required are at least 100 fold lower. Our results suggest that VAS/VEGF could be an important regulator of osteoblastic differentiation.
Midy
V
V
Laboratoire de Biologie Moléculaire Eucaryote, UPR 9006 CNRS, Toulouse, France.
Plouët
J
J
D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D016228:Endothelial Growth Factors / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D006133:Growth Substances; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2; D000469:Alkaline Phosphatase