Changes in the hybridization patterns of populations of Theileria sergenti during infection.
Restriction fragment length polymorphisms (RFLPs) of Theileria sergenti DNA were analysed using probes of a genomic DNA fragment (pTs 2) and a cDNA corresponding to this genomic probe (C-Ts 2). Each of the probes detected RFLPs in DNA from different stocks of Theileria sergenti. Additionally, using these probes, alterations in hybridization patterns were observed in samples of the parasites harvested at different times after individual calves had been infected with Theileria sergenti. This result suggests that the Theileria sergenti stocks used were mixed parasite populations.
Matsuba
T
T
Department of Veterinary Microbiology, Rakuno Gakuen University, Ebetsu, Japan.
Sugimoto
C
C
Onoe
S
S
Kawakami
Y
Y
Iwai
H
H
Onuma
M
M
D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology; D015139:Blotting, Southern / Q000662:veterinary; D002417:Cattle; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis* / Q000302:isolation & purification; D015723:Gene Library; D014644:Genetic Variation; D009693:Nucleic Acid Hybridization / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D016053:RNA, Protozoan / Q000302:isolation & purification; D016794:Theileria / Q000235:genetics*; D013801:Theileriasis / Q000469:parasitology*; D013987:Ticks / Q000469:parasitology
D015342:DNA Probes; D016054:DNA, Protozoan; D016053:RNA, Protozoan