A phorbol ester-responsive PKC-zeta generated by fusion with the regulatory domain of PKC-delta.
A hybrid molecule generated by fusing the regulatory domain of PKC-delta with the catalytic domain of PKC-zeta is, like PKC-delta but unlike PKC-zeta, a phorbol ester-dependent enzyme. However, the substrate specificity of this hybrid resembles that of PKC-zeta. Expression of mammalian PKC-delta, but not PKC-zeta, in the fission yeast Schizosaccharomyces pombe causes growth retardation and phorbol esters amplify the PKC-delta phenotype without affecting that of PKC-zeta (Goode et al., submitted). The chimaeric molecule also inhibited growth and this effect was phorbol-ester dependent. Both the hybrid and PKC-delta holoenzyme, in contrast to PKC-zeta, down-regulate upon prolonged exposure to phorbol esters in vivo. Thus, this hybrid retains the regulatory properties conferred by PKC-delta but the catalytic properties of PKC-zeta. This regulatable chimaeric molecule will be useful in assessing the function of PKC-zeta.
Goode
N T
NT
Department of Veterinary Basic Sciences, Royal Veterinary College, London, UK.
Parker
P J
PJ
D004790:Enzyme Induction / Q000187:drug effects; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D011493:Protein Kinase C / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate