Inter-institutional reproducibility of flow cytometric DNA-analysis in breast carcinomas.
In order to study interinstitutional reproducibility of flow cytometric DNA-analysis (DNA-FCM), frozen pieces from 30 consecutive breast carcinomas were analysed by 5 laboratories. Different instruments, preparation and DNA staining methods were used. A concordance in DNA-ploidy status was obtained in 26 of the 30 tumours. The discrepancy can mainly be explained by intratumoural DNA-heterogeneity since a complete agreement in ploidy status was obtained when four of the laboratories analysed the same cell suspension, where solid bits showed differing results. The sampling method seems therefore to be a crucial step for the results and needs further studies. As far as the estimation of S-phase fraction was concerned, one laboratory obtained significantly higher values compared to the other four. The correlation between the other four laboratories varied between r = 0.66-0.92.
Risberg
B
B
Department of Pathology, Medical Center Hospital, Orebro, Sweden.
Baldetorp
B
B
Fernö
M
M
Hansson
B
B
Mossberg
L L
LL
Roos
G
G
Stål
O
O
Thornthwaite
J T
JT
Tibell
L
L
D000047:Academies and Institutes / Q000592:standards; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D011003:Ploidies; D012044:Regression Analysis; D015203:Reproducibility of Results; D016196:S Phase
D004273:DNA, Neoplasm