A radiation hybrid map of the BRCA1 region.
A locus on chromosome 17q, designated "BRCA1," has been identified as a predisposition gene for breast cancer. A panel of chromosome 17-specific radiation-reduced somatic cell hybrid clones has been assembled for high-resolution mapping of chromosome 17. A series of 35 markers, known to span the BRCA1 locus, were tested against this hybrid panel by PCR assays. Statistical analysis of these data yields a BRCA1 radiation hybrid map at a density sufficient to initiate YAC cloning and pulsed-field gel electrophoretic mapping of the candidate region. In addition, many of the markers reveal genetic polymorphisms and may be tested in breast cancer families and in loss-of-heterozygosity studies of sporadic breast cancers to better define the BRCA1 gene candidate region.
O'Connell
P
P
Department of Pathology, University of Texas Health Science Center, San Antonio 78284.
Albertsen
H
H
Matsunami
N
N
Taylor
T
T
Hundley
J E
JE
Johnson-Pais
T L
TL
Reus
B
B
Lawrence
E
E
Ballard
L
L
White
R
R
D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D006528:Carcinoma, Hepatocellular; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D017931:DNA Primers; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D051381:Rats; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D005819:Genetic Markers