title: The cloning of CD70 and its identification as the ligand for CD27.
abstract: CD70 is a surface Ag found on activated but not resting T and B lymphocytes. The biologic activity of this Ab-defined cell surface molecule on lymphocytes has not been established. Therefore, in an effort to understand the function of the CD70 protein, a mAb defining the CD70 Ag was used to isolate by expression cloning the cDNA responsible for the CD70 molecule. The predicted protein product is a type II transmembrane protein. Bioassays demonstrated that the CD70 cDNA clone expressed in African green monkey kidney cells would induce the proliferation of PHA-costimulated T cells. Comparison with known sequences indicates identity with the CD27 ligand. Therefore the molecule defining the CD70 Ag is identical to the recently defined ligand for CD27.
authors.affiliation: Genetics Institute, Cambridge, MA 02140.
authors.forename: M R
authors.initials: MR
authors.lastname: Bowman
authors.forename: M A
authors.initials: MA
authors.lastname: Crimmins
authors.forename: J
authors.initials: J
authors.lastname: Yetz-Aldape
authors.forename: R
authors.initials: R
authors.lastname: Kriz
authors.forename: K
authors.initials: K
authors.lastname: Kelleher
authors.forename: S
authors.initials: S
authors.lastname: Herrmann
mesh_terms: D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D053282:CD27 Ligand; D002460:Cell Line; D003001:Cloning, Molecular; D006801:Humans; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D053282:CD27 Ligand; C505771:CD70 protein, human; D008565:Membrane Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D013755:Tetradecanoylphorbol Acetate