title: [Importance of the colon in intestinal adaptation. Study with the proliferating cell nuclear antigen].
abstract: Proliferating cell nuclear antigen (PCNA) is an auxiliary protein to DNA polymerase delta necessary for tissue cellular proliferation. The colon releases several peptides or hormones which are probably related to intestinal proliferation. Colonic resection determines adaptive changes in the remnant bowel. In the present study, proliferative changes after colectomy were studied by means of the murine monoclonal PC10 antibody. A control group (n = 10 rats) and a 75% proximal colon resection group (n = 10 rats) were studied. 14 days after resection, jejunal, ileal and colon samples were taken and assayed for PCNA. Relationship between immunostained nuclei and the total number of nuclei was determined. The three intestinal segments showed statistically significant increases (p < 0.001) in the number of immunostained nuclei. PCNA proliferative index was greater in the remnant large bowel.
authors.affiliation: Servicio de Cirugía Experimental, Hospital La Paz, Madrid.
authors.forename: A
authors.initials: A
authors.lastname: Mata
authors.forename: I A
authors.initials: IA
authors.lastname: Gómez de Segura
authors.forename: J
authors.initials: J
authors.lastname: Díaz
authors.forename: L
authors.initials: L
authors.lastname: Santamaría
authors.forename: J
authors.initials: J
authors.lastname: Codesal
authors.forename: E
authors.initials: E
authors.lastname: De Miguel
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001324:Autoantigens / Q000032:analysis; D002452:Cell Count; D002455:Cell Division; D003082:Colectomy; D003106:Colon / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D007082:Ileum / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D007583:Jejunum / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D008940:Mitotic Index; D009687:Nuclear Proteins / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001324:Autoantigens; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen
vernacular_title: Importancia del colon en la adaptación intestinal. Estudio mediante el antígeno nuclear de proliferación celular.