title: Comparison of typing methods for Clostridium difficile isolates.
abstract: A simple discriminative typing method for Clostridium difficile has been developed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins and restriction enzyme analysis are relatively simple techniques but are difficult to evaluate, especially the restriction enzyme analysis. Immunoblotting and restriction fragment length polymorphism typing facilitate simple discrimination of patterns.
authors.affiliation: Eijkman-Winkler Institute for Medical and Clinical Microbiology, University Hospital Utrecht, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Wolfhagen
authors.forename: A C
authors.initials: AC
authors.lastname: Fluit
authors.forename: R
authors.initials: R
authors.lastname: Torensma
authors.forename: M
authors.initials: M
authors.lastname: Jansze
authors.forename: A F
authors.initials: AF
authors.lastname: Kuypers
authors.forename: E A
authors.initials: EA
authors.lastname: Verhage
authors.forename: J
authors.initials: J
authors.lastname: Verhoef
mesh_terms: D015373:Bacterial Typing Techniques; D002648:Child; D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics / Q000276:immunology; D004262:DNA Restriction Enzymes; D006801:Humans; D015151:Immunoblotting; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004262:DNA Restriction Enzymes