title: Hepatic lipase mRNA is expressed in rat and human steroidogenic organs.
abstract: Rat and human steroidogenic organs contain an enzyme activity that is indistinguishable from hepatic lipase present in liver. Using primers that recognize exons 5 and 8 of the rat and human HL gene, a 596-bp product was found by RT-PCR in rat liver, adrenal, ovaries and testes, but not in heart and kidney. A similar product was also observed with human hyperplastic adrenocortical tissue. Identity of this product with part of the HL cDNA was confirmed by restriction mapping and internal re-amplification. Our results indicate that the HL gene is transcribed in steroidogenic tissues that also contain HL protein.
authors.affiliation: Department of Biochemistry, Erasmus University Rotterdam, The Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: Verhoeven
authors.forename: H
authors.initials: H
authors.lastname: Jansen
mesh_terms: D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D005260:Female; D006801:Humans; D008049:Lipase / Q000235:genetics* / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D015183:Restriction Mapping; D013737:Testis / Q000201:enzymology
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D008049:Lipase