title: Characterization of the gene encoding the human transcriptional elongation factor TFIIS.
abstract: The transcriptional elongation factor TFIIS causes stimulation of RNA polymerase II elongation and readthrough of some of the elongation blocks. We present cloning and sequence characterization of the human TFIIS gene and a pseudogene. The intron-less organization of both of these genes indicates that previously identified cDNAs which suggested the presence of an intron were the products of cloning artifacts. The gene is organized in an uninterrupted ORF which codes for 301 amino acids, whereas the pseudogene lacks an ORF able to code for a full-length protein. The potential promoter for the gene has two putative GC-box-type consensus sequences, two CCAAT-box consensus sequences, and is bounded by a human Alu sequence. Two potential transcriptional termination signal sequences downstream from the consensus polyadenylation signal are proposed.
authors.affiliation: Department of Life Science, Korea Advanced Institute of Science and Technology, Taejon.
authors.forename: H
authors.initials: H
authors.lastname: Park
authors.forename: K
authors.initials: K
authors.lastname: Baek
authors.forename: C
authors.initials: C
authors.lastname: Jeon
authors.forename: K
authors.initials: K
authors.lastname: Agarwal
authors.forename: O
authors.initials: O
authors.lastname: Yoo
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011544:Pseudogenes / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D014157:Transcription Factors; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II