Protein EnvM is the NADH-dependent enoyl-ACP reductase (FabI) of Escherichia coli.
The EnvM protein was purified from an overproducing Escherichia coli strain. It showed NADH-dependent enoyl-acyl carrier protein (ACP) reductase activity using both crotonyl-ACP and crotonyl-CoA as substrates. The protein bound a radioactive diazaborine derivative in the presence of NAD+ and radioactive NAD+ in the presence of the drug. Based on these data, it is concluded that EnvM is the NADH-dependent enoyl-ACP reductase (EC 1.3.1.9) of E. coli and we propose to rename the corresponding gene fabI.
Bergler
H
H
Institut für Mikrobiologie, Universität Graz, Austria.
Wallner
P
P
Ebeling
A
A
Leitinger
B
B
Fuchsbichler
S
S
Aschauer
H
H
Kollenz
G
G
Högenauer
G
G
Turnowsky
F
F
D000214:Acyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D050753:Enoyl-(Acyl-Carrier-Protein) Reductase (NADH); D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D054889:Fatty Acid Synthase, Type II; D007700:Kinetics; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D004364:Pharmaceutical Preparations; D009243:NAD; C010701:crotonyl-coenzyme A; D010088:Oxidoreductases; D050753:Enoyl-(Acyl-Carrier-Protein) Reductase (NADH); C493924:fabI protein, E coli; D054889:Fatty Acid Synthase, Type II