title: Rapid detection of PCR products of hepatitis C virus (HCV) with a non-radioactive oligoprobe.
abstract: Dot-blot for rapid detection of PCR amplification products of hepatitis C virus (HCV) using a digoxigenin (DIG)-labelled oligoprobe was developed and its sensitivity compared with Southern blot hybridization. The specificity and sensitivity of the DIG-labelled probe were identical to those of the 32P-labelled when the DIG-labelled nucleic acids were detected by enzyme-catalyzed chemiluminescent reaction. The lack of radioactivity makes this procedure suitable for routine use in diagnostic laboratories.
authors.affiliation: Istituto di Microbiologia dell'Università di Verona, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Canepari
authors.forename: C F
authors.initials: CF
authors.lastname: Di Stefano
authors.forename: M M
authors.initials: MM
authors.lastname: Lleò
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002908:Chronic Disease; D004076:Digoxigenin; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood; D012680:Sensitivity and Specificity
chemical_list: D015345:Oligonucleotide Probes; D012367:RNA, Viral; D004076:Digoxigenin