Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus.
The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV) strain Smith was determined and compared with the sequence of the gH of MCMV strain K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5.0 kb, which is synthesized late in infection. A recombinant vaccinia virus expressing the MCMV gH open reading frame was constructed (Vac-gH). Anti-MCMV serum precipitated a protein of 87K from Vac-gH-infected cells. Reactivity with a monoclonal antibody showed the identity of the MCMV gH with a 87K envelope glycoprotein described previously by Loh and Qualtiere. Immunization of mice with the Vac-gH recombinant gave rise to an anti-gH serum, which neutralized MCMV without complement in vitro.
Rapp
M
M
Department of Virology, University of Ulm, Germany.
Lucin
P
P
Messerle
M
M
Loh
L C
LC
Koszinowski
U H
UH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005814:Genes, Viral / Q000235:genetics; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007114:Immunization; D051379:Mice; D008969:Molecular Sequence Data; D018146:Muromegalovirus / Q000235:genetics* / Q000276:immunology; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D014616:Vaccinia virus / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D006023:Glycoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins