Assignment of human satellite 1 DNA as revealed by fluorescent in situ hybridization with oligonucleotides.
We have used a fluorescent in situ hybridization procedure to detect human satellite 1 DNA, the simple sequence family that constitutes the non-male-specific fraction of classical satellite 1 DNA. Satellite 1 appears to be located on pericentromeric regions of chromosomes 3, 4 and 13, and on satellites of each acrocentric chromosome. These results suggest a possible relationship between quinacrine fluorescence of heterochromatin and DNA composition. Furthermore, by means of multicolour in situ hybridization, we have spatially resolved satellite 1 sequences and centromeric alpha-satellite within heterochromatic blocks.
Tagarro
I
I
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Wiegant
J
J
Raap
A K
AK
González-Aguilera
J J
JJ
Fernández-Peralta
A M
AM
D001483:Base Sequence; D002503:Centromere / Q000737:chemistry; D002882:Chromosomes, Human, Pair 13 / Q000737:chemistry*; D002893:Chromosomes, Human, Pair 3 / Q000737:chemistry*; D002894:Chromosomes, Human, Pair 4 / Q000737:chemistry*; D016384:Consensus Sequence; D004276:DNA, Satellite / Q000032:analysis*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes
D004276:DNA, Satellite; D015345:Oligonucleotide Probes