title: DNA ligase I is associated with the 21 S complex of enzymes for DNA synthesis in HeLa cells.
abstract: Approximately 80% of the DNA ligase activity in HeLa cell extracts is associated with the 21 S enzyme complex that functions in simian virus 40 DNA replication in vitro (Malkas et al., Biochemistry 29, 6362-6374., 1990). The DNA ligase associated with the 21 S complex was purified extensively and its physical, enzymic and immunological properties characterized as DNA ligase I. The association of DNA ligase I with the 21 S complex of enzymes for DNA synthesis provides evidence for the physiological function of this DNA ligase in DNA replication in human cells.
authors.affiliation: Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545.
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Goodchild
authors.forename: E F
authors.initials: EF
authors.lastname: Baril
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000378:metabolism*; D004261:DNA Replication / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism*; D013539:Simian virus 40 / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics
chemical_list: D004279:DNA, Viral; C000607892:LIG1 protein, human; D009097:Multienzyme Complexes; D011088:DNA Ligases; D000072481:DNA Ligase ATP