Role of endoglucanases in Erwinia chrysanthemi 3937 virulence on Saintpaulia ionantha.
The role of endoglucanases (endoglucanases Z and Y) in Erwinia chrysanthemi pathogenicity on Saintpaulia ionantha was assessed by mutagenizing cloned cel genes (celZ and celY) and recombining them with the chromosomal alleles. Strains with an omega interposon in celZ, a deletion in celY, or a double cel mutant were as virulent as the wild-type strain. However, in the strain with a deletion in celY, a delay in the appearance of symptoms was observed, and then maceration progressed as in plants infected with the wild-type strain, suggesting that E. chrysanthemi endoglucanases play a minor role in soft rot disease development.
Boccara
M
M
Laboratoire de Pathologie Végétale, Institut National de la Recherche Agronomique, Paris, France.
Aymeric
J L
JL
Camus
C
C
D000483:Alleles; D002480:Cellulase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D016974:Dickeya chrysanthemi / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D005798:Genes, Bacterial; D016254:Mutagenesis, Insertional; D010944:Plants / Q000382:microbiology*; D011995:Recombination, Genetic; D015183:Restriction Mapping; D017384:Sequence Deletion
C080692:endoglucanase Z; D002480:Cellulase