Corticotropin-releasing hormone enhances the superoxide anion production of rabbit peritoneal macrophages stimulated with N-formyl-methionyl-leucyl-phenylalanine.
Effects of corticotropin-releasing hormone (CRH) on the superoxide anion (O2-) production in macrophages (M phi s) were investigated. CRH by itself failed to induce the O2- production of rabbit peritoneal M phi s elicited with thioglycollate medium. However, M phi s preincubated with CRH showed significant enhancement of O2- production following stimulation with a chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (FMLP), at CRH concentrations from 10(-10) to 10(-7) M. Maximal enhancement of O2- production was obtained with 10 min preincubation with CRH for FMLP stimulation. CRH had no effect on O2- production when stimulated with phorbol 12-myristate 13-acetate. Neither corticotropin nor beta-endorphin augmented the O2- production induced with FMLP. These results indicate that CRH has priming effects on FMLP-induced O2- production of rabbit peritoneal M phi s, although the underlying mechanism remains to be clarified.
Koshida
H
H
National Biomedical Center for Spin Trapping and Free Radicals, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Kotake
Y
Y
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D005260:Female; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D011817:Rabbits; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D001615:beta-Endorphin / Q000494:pharmacology
D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D013755:Tetradecanoylphorbol Acetate