Isolation and mapping of discrete DXS101 loci in Xq22 near the X-linked agammaglobulinaemia gene locus.
The X-linked agammaglobulinaemia (XLA) gene locus has previously been mapped to Xq22 in genetic linkage studies. The DXS101 locus has shown no recombinations with XLA in the ten informative meioses investigated so far. The DXS101 sequence, recognised by the cX52.5 plasmid, is moderately repeated in Xq22. We have isolated cosmids which contain this sequence; two copies of which have been found to lie near DXS178 and XLA, and a third copy which lies near the PLP gene, distal to these loci. We have used the cosmids to generate probes which should be of use for RFLP analysis, and thus in both prenatal diagnosis and carrier testing for XLA, and in constructing a genetic map of this region. These probes will also be used to complement the genetic map in the construction of a complete physical map of Xq22.
O'Reilly
M A
MA
Molecular Immunology Unit, Institute of Child Health, London, UK.
Sweatman
A K
AK
Bradley
L D
LD
Alterman
L A
LA
Lovering
R
R
Malcolm
S
S
Levinsky
R J
RJ
Kinnon
C
C
D000361:Agammaglobulinemia / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003360:Cosmids; D008040:Genetic Linkage; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D014960:X Chromosome