Determination of nefazodone and its metabolites in plasma by high-performance liquid chromatography with coulometric detection.
Nefazodone and its metabolites together with the internal standard are extracted from plasma using CN Bond-Elut sorbent columns. Chromatography and detection are performed using isocratic reversed-phase high-performance liquid chromatography (HPLC) with a CN column and coulometric end-point detection. The lower limits of quantitation for nefazodone, mCPP, and OH-nefazodone was 0.9, 0.2 and 0.6 ng ml-1 when 1 ml of plasma was extracted. The interassay relative standard deviations were less than 6% for nefazodone and its metabolites. Recovery averaged over 80%. Plasma profiles of nefazodone and its metabolites following oral dosing are presented.
Franklin
M
M
University of Oxford Department of Psychiatry, Littlemore Hospital, UK.
D000284:Administration, Oral; D000928:Antidepressive Agents / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D010879:Piperazines; D012015:Reference Standards; D012680:Sensitivity and Specificity; D014230:Triazoles / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism
D000928:Antidepressive Agents; D010879:Piperazines; D014230:Triazoles; C051752:nefazodone