Pneumocystis carinii pneumonia in thoroughbred foals: identification of a genetically distinct organism by DNA amplification.
Genetically distinct forms of Pneumocystis carinii infect several mammalian hosts. We report the amplification of P. carinii DNA from samples of two infected thoroughbred foal lungs by using primers designed from the sequence of a P. carinii mitochondrial rRNA gene; these primers also prime the amplification of P. carinii DNA from other hosts. The nucleotide sequence of part of the mitochondrial rRNA gene amplified from P. carinii infecting one of the foals was determined and found to be distinct from that of published rat-, rabbit-, ferret-, and human-derived P. carinii sequences.
Peters
S E
SE
Department of Paediatrics, Radcliffe Hospital, Oxford, United Kingdom.
Wakefield
A E
AE
Whitwell
K E
KE
Hopkin
J M
JM
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D005289:Ferrets; D005784:Gene Amplification; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D006801:Humans; D008168:Lung / Q000382:microbiology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011010:Pneumocystis / Q000145:classification / Q000235:genetics*; D011020:Pneumonia, Pneumocystis / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D011817:Rabbits; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D017931:DNA Primers; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal