title: Uncoupled steps of the colicin A pore formation demonstrated by disulfide bond engineering.
abstract: Four disulfide bonds were engineered into the pore-forming domain of colicin A to probe the conformational changes associated with its membrane insertion and channel formation. The soluble pore-forming domain consists of 10 alpha-helices with two outer layers (helices 1, 2, and 3-7, respectively) sandwiching a middle layer of three helices (8-10). Helices 8 and 9 form a hairpin which is completely buried and consists of hydrophobic and neutral residues only. This helical hairpin has been hypothesized to be the membrane anchor. Each double-cysteine mutant possessing an individual disulfide bond, cross-linking either helices 1 to 9 (H1/H9), 5 to 6 (H5/H6), 7 to 8 (H7/H8), or 9 to 10 (H9/H10), respectively, is unable to promote K+ efflux from sensitive Escherichia coli cells. Activity can be restored by addition of a reducing agent. In vitro studies with brominated lipid vesicles and planar lipid bilayers show that the disulfide bond which connects the helices 1 to 9 prevents colicin A membrane insertion, whereas the other disulfide bond mutants insert readily into lipid vesicles. All of the engineered bridges prevented the formation of a conducting channel in the presence of a membrane potential. This novel approach indicates that membrane insertion and channel formation are two separate steps. Moreover, the effects of the distance constraints introduced by the different disulfide bonds on colicin A activity indicate that the helical pair 1 and 2 moves away from the other helices upon membrane insertion. Helices 3-10 remain associated together. As a consequence, the results imply that the helical hairpin lies parallel to the membrane surface. In contrast, induction of the colicin channel by the membrane potential requires a profound reorganization of the helices association. These results are discussed in light of several proposed models of the membrane-bound colicin and channel structures.
authors.affiliation: Laboratoire d'Ingénierie et de Dynamique des Systèmes Membranaires, Centre de Biochimie et de Biologie Moléculaire, Marseille, France.
authors.forename: D
authors.initials: D
authors.lastname: Duché
authors.forename: M W
authors.initials: MW
authors.lastname: Parker
authors.forename: J M
authors.initials: JM
authors.lastname: González-Mañas
authors.forename: F
authors.initials: F
authors.lastname: Pattus
authors.forename: D
authors.initials: D
authors.lastname: Baty
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003087:Colicins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D003545:Cysteine; D004220:Disulfides / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008051:Lipid Bilayers; D008081:Liposomes; D008961:Models, Structural; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D010957:Plasmids; D015202:Protein Engineering; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D015183:Restriction Mapping
chemical_list: D003087:Colicins; D004220:Disulfides; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D011994:Recombinant Proteins; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol; C024990:asolectin; D003545:Cysteine