title: Mouse JunD negatively regulates fibroblast growth and antagonizes transformation by ras.
abstract: As NIH 3T3 fibroblasts become quiescent, the level of c-Jun protein decreases while JunD accumulates. When resting cells are stimulated with fresh serum, nuclear-localized JunD is rapidly degraded, followed by resynthesis of both c-Jun and JunD later in G1. Overexpression of JunD results in slower growth and an increase in the percentage of cells in G0/G1 while c-Jun overexpression produces larger S/G2 and M phase populations. In addition, JunD partially suppresses transformation by an activated ras gene whereas c-Jun cooperates with ras to transform cells. These data indicate that two closely related transcription factors can function in an opposing manner.
authors.affiliation: Département des Biotechnologies, Institut Pasteur, Paris, France.
authors.forename: C M
authors.initials: CM
authors.lastname: Pfarr
authors.forename: F
authors.initials: F
authors.lastname: Mechta
authors.forename: G
authors.initials: G
authors.lastname: Spyrou
authors.forename: D
authors.initials: D
authors.lastname: Lallemand
authors.forename: S
authors.initials: S
authors.lastname: Carillo
authors.forename: M
authors.initials: M
authors.lastname: Yaniv
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D005347:Fibroblasts / Q000166:cytology; D011905:Genes, ras; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics* / Q000378:metabolism
chemical_list: D010455:Peptides; D016755:Proto-Oncogene Proteins c-jun