Detection by capillary electrophoresis of restriction fragment length polymorphism. Analysis of a polymerase chain reaction-amplified product of the DXS 164 locus in the dystrophin gene.
Capillary electrophoresis (CE) was used to characterize restriction fragment length polymorphism (RFLP) in a polymerase chain reaction (PCR)-amplified product of a 740-base pairs DNA fragment from the DXS 164 locus of the dystrophin gene. The polymorphic alleles of 740 and 520/220 base pairs revealed by XmnI digestion were analysed from homozygous and heterozygous individuals by CE. Our studies show that extraction in phenol-chloroform may be useful in PCR-amplified product purification. Excellent separation was obtained in a short time. The data indicate that CE is suitable for genomic analysis such as carrier detection and prenatal diagnosis of X-linked recessive disorders after purification of PCR-amplified products.
Del Principe
D
D
Dipartimento di Sanità Pubblica e Biologia Cellulare, Facoltà di Medicina, Università di Roma Tor Vergata, Italy.
Iampieri
M P
MP
Germani
D
D
Menichelli
A
A
Novelli
G
G
Dallapiccola
B
B
D000328:Adult; D001483:Base Sequence; D002021:Buffers; D016189:Dystrophin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004586:Electrophoresis; D004587:Electrophoresis, Agar Gel; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D002021:Buffers; D016189:Dystrophin; D005819:Genetic Markers