Changes in cell three-dimensional locomotory characteristics upon expression of the EJ-ras oncogene.
Activation of the ras oncogene induces ruffling of membranes, enhances cell locomotion and has been implicated in the invasive process. We have investigated the locomotory changes of a 212 cell line due to ras activation using a novel system for determining the three-dimensional trajectories of cells migrating within collagen lattices. It was found that a small, yet statistically significant increase in cell speed resulted and that the duration of locomotory periods was prolonged in ras activated cells. These locomotory periods was prolonged in ras activated cells. These locomotory periods were cyclic in nature with a periodicity of approximately 30 min. These results are discussed in the relation to the current hypotheses concerning the molecular mechanisms involved in pseudopod kinetics.
Noble
P B
PB
Department of Oral Biology, Faculty of Dentistry, McGill University, Montreal, Canada.
Shields
E D
ED
Walton
P A
PA
D015153:Blotting, Western; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D003094:Collagen / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D011905:Genes, ras / Q000235:genetics / Q000502:physiology; D009361:Neoplasm Invasiveness / Q000235:genetics; D015513:Oncogene Proteins / Q000235:genetics / Q000502:physiology*
D015513:Oncogene Proteins; C077183:human oncogene protein EJ-ras; D003094:Collagen