New markers for linkage analysis of X-linked hypophosphataemic rickets.
Three polymorphic markers have been used to improve the genetic map of the region Xp22.1-p22.2, which contains the HYP (hypophosphataemic rickets) locus. DXS365 gave no recombinants with HYP, with a peak Lod score of 5.4 at theta = 0.0. A microsatellite marker mPA274 was derived for the DXS274 locus; it detects five alleles with a polymorphism information content of 0.55. Combining information from this microsatellite and the original DXS274 marker, probe CRI-L1391, the peak Lod score for DXS274 against HYP was 9.6 at theta = 0.02. A microsatellite associated with the DXS207 locus (mPA207) gave a peak lod score against HYP of 4.7 at theta = 0.14. A consideration of key recombinants and multilocus analysis suggests the gene order. Xpter-DXS207-DXS43-DXS197-(DXS365,HYP)- DXS274-DXS41-Xcen.
Rowe
P S
PS
Middlesex Hospital, London, UK.
Goulding
J
J
Read
A
A
Mountford
R
R
Hanauer
A
A
Oudet
C
C
Whyte
M P
MP
Meier-Ewert
S
S
Lehrach
H
H
Davies
K E
KE
D001483:Base Sequence; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D007015:Hypophosphatemia, Familial / Q000235:genetics*; D008126:Lod Score; D008297:Male; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D016324:Sequence Tagged Sites; D014960:X Chromosome
D005819:Genetic Markers