A comparison of immunoglobulin G-containing high-molecular-weight complexes isolated from children with juvenile rheumatoid arthritis and congenital human immunodeficiency virus infection.
Circulating immune complexes have been described in both juvenile rheumatoid arthritis and in AIDS. We isolated high-molecular-weight complexes from plasma of children with juvenile rheumatoid arthritis and congenital human immunodeficiency virus infection by sequential gel filtration followed by affinity chromatography on protein A-Sepharose. We demonstrate that 1) mixed IgG-, IgM-, and IgA-bearing immune complexes can be found in both juvenile rheumatoid arthritis and congenital human immunodeficiency virus infection and 2) complexes isolated via affinity chromatography on protein A-Sepharose do not have detectable C4 but activate the classic complement pathway in vitro.
Jarvis
J N
JN
Division of Immunology/Rheumatology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Iobidze
M
M
Taylor
H
H
DeJonge
J
J
Chang
S
S
D000293:Adolescent; D000936:Antigen-Antibody Complex / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D001171:Arthritis, Juvenile / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity; D003181:Complement C4 / Q000378:metabolism; D015935:Complement C4b; D003171:Complement Pathway, Classical; D015658:HIV Infections / Q000151:congenital / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood; D066298:In Vitro Techniques; D007223:Infant; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism; D012217:Rheumatoid Factor / Q000097:blood
D000936:Antigen-Antibody Complex; D003181:Complement C4; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010446:Peptide Fragments; D015935:Complement C4b; C032261:complement C4d; D012217:Rheumatoid Factor