Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins.
Four of the tetraspans family antigens expressed in B cells, CD37, CD53, TAPA-1, and R2/C33, as well as at least two other molecules, CD19 and CD21, coprecipitate with DR antigens from mild detergent lysates of human B-cell lines and tonsillar B cells. Coprecipitation and preclearing experiments indicate the existence of large multicomponent complexes containing jointly the seven components, although some "incomplete" complexes lacking some of the components may also exist. The complexes contain only a relatively small fraction of the total cellular pool of relevant molecules. The existence of these "tetraspans-DR complexes" may be related to the previously reported antiproliferative and signaling effects of antibodies against most of their components.
Angelisová
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Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Praha.
Hilgert
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Horejsí
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D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000302:isolation & purification / Q000378:metabolism; D000943:Antigens, Differentiation / Q000302:isolation & purification / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000302:isolation & purification / Q000378:metabolism; D000951:Antigens, Neoplasm; D000954:Antigens, Surface / Q000302:isolation & purification / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism; D006684:HLA-DR Antigens / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D051861:Kangai-1 Protein; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011233:Precipitin Tests; D011518:Proto-Oncogene Proteins; D060265:Tetraspanin 25; D060225:Tetraspanin 28; D059470:Tetraspanins
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; C059071:CD37 protein, human; C554894:CD53 protein, human; C554798:CD81 protein, human; C497688:CD82 protein, human; D006023:Glycoproteins; D006684:HLA-DR Antigens; D051861:Kangai-1 Protein; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D060265:Tetraspanin 25; D060225:Tetraspanin 28; D059470:Tetraspanins