title: Heterogeneity and stability characteristics of Candid 1 attenuated strain of Junin virus.
abstract: Fourteen clones of Candid 1 strain of Junin virus were studied in terms of their attenuation and stability for 9 days-old mice. The cluster analysis of the efficiency of lethal infection, namely the virulence (lethality) indicates (PFU/LD50) of the clones showed that the strain population is heterogeneous. It is constituted by subpopulations with lower and higher virulence as compared to the parent strain. Moreover, one or two passages through mice and Calomys musculinus, the main Junin virus reservoir of this strain produced changes of its virulence meanwhile the in vitro passages did not affect its attenuation level.
authors.affiliation: Instituto de Virologia Dr. J. M. Vanella, Facultad de Ciencias Medicas Universidad Nacional de Cordoba, Ciudad Universitaria, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Contigiani
authors.forename: S
authors.initials: S
authors.lastname: Medeot
authors.forename: G
authors.initials: G
authors.lastname: Diaz
mesh_terms: D000818:Animals; D004355:Drug Stability; D014644:Genetic Variation; D006168:Guinea Pigs; D018051:Junin virus / Q000276:immunology* / Q000472:pathogenicity; D051379:Mice; D014613:Vaccines, Attenuated; D014709:Vero Cells; D010948:Viral Plaque Assay; D014774:Virulence
chemical_list: D014613:Vaccines, Attenuated