Magnolol and honokiol isolated from Magnolia officinalis protect rat heart mitochondria against lipid peroxidation.
In isolated rat heart mitochondria lipid peroxidation was induced with ADP and ferrous sulfate (FeSO4). Oxygen consumption and malondialdehyde (MDA) production were measured to quantitate lipid peroxidation. The antioxidant effects of magnolol and honokiol purified from Magnolia officinalis were 1000 times higher than that of alpha-tocopherol. The IC50 values of magnolol and honokiol for inhibition of oxygen consumption were 8.0 x 10(-8) M and 1.0 x 10(-7) M, respectively, while that of alpha-tocopherol was 1.0 x 10(-4) M. Magnolol at 0.5 microM inhibited 71.4 +/- 9.4% of oxygen consumption and 59.3 +/- 4.6% MDA production. At the same concentration, honokiol inhibited 78.1 +/- 4.7% of oxygen consumption and 86.9 +/- 4.0% of MDA production. Of conjugated diene formation 48.4 +/- 4.6% and 53.1 +/- 3.4% were inhibited by 0.5 microM magnolol and honokiol, respectively. Also both magnolol and honokiol exhibited free radical scavenging activities as shown by the diphenyl-p-picrylhydrazyl assay, but they were less potent than alpha-tocopherol.
Lo
Y C
YC
Institute of Traditional Medicine, Yang-Ming Medical College, Taipei, Taiwan, ROC.
Teng
C M
CM
Chen
C F
CF
Chen
C C
CC
Hong
C Y
CY
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D017705:Lignans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008929:Mitochondria, Heart / Q000187:drug effects*; D010101:Oxygen Consumption; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000494:pharmacology; D000498:Allyl Compounds; D010636:Phenols
D000975:Antioxidants; D001713:Biphenyl Compounds; D017705:Lignans; D008315:Malondialdehyde; D014810:Vitamin E; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols