The calmodulin-binding domain of caldesmon binds to calmodulin in an alpha-helical conformation.
The binding of calcium-calmodulin (CaM) to caldesmon (CaD) contributes to the regulation of smooth muscle contraction. It has been reported that a 17-residue synthetic peptide encompassing the residues Gly651-Ser667 of smooth muscle CaD constitutes its CaM-binding domain [Zhan, Q., Wong, S. S., & Wang, C.L.A. (1991) J. Biol. Chem. 266, 21810-21814]. This peptide does not share sequence homology with the CaM-binding domains of other proteins, and in addition, the binding of CaM to CaD is known to be relatively weak. Here we have investigated the properties of this atypical CaM-binding domain by NMR and circular dichroism (CD) spectroscopy. Two dimensional NMR studies performed in aqueous TFE mixture (75%/25%) showed that the peptide has the capacity to adopt an amphiphilic alpha-helical conformation. TRNOESY experiments and CD spectroscopy were used to determine that the CaD peptide binds in an alpha-helical conformation to CaM. The addition of TFE or the binding of the CaD peptide to CaM induces an alpha-helical structure only for the central 10 amino acid residues of the peptide. Titrations of CaM with the CaD peptide were followed by proton NMR and show the formation of a 1:1 complex and that the binding is calcium-dependent. The chemical shifts of 13C-methyl groups of specifically labeled Met residues and of the 15N backbone amide groups of CaM undergo changes upon addition of the CaD peptide; these data suggest that both domains and the central helix of CaM are involved in the binding of the peptide.(ABSTRACT TRUNCATED AT 250 WORDS)
Zhang
M
M
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Vogel
H J
HJ
D000595:Amino Acid Sequence; D001665:Binding Sites; D002147:Calmodulin / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011487:Protein Conformation
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins