title: The fadD gene of Escherichia coli K12 is located close to rnd at 39.6 min of the chromosomal map and is a new member of the AMP-binding protein family.
abstract: The fadD gene of Escherichia coli K12 was cloned and sequenced. The gene was identified by its ability to complement the corresponding mutant and by measuring the enzymatic activity after its expression in this mutant. The deduced polypeptide sequence exhibits similarity to other long chain acyl-CoA (coenzyme A) synthetases and a variety of other proteins, which together form a family of AMP-binding proteins. This family is extended by several new members and subdivided into four groups. fadD is assigned to a subgroup that does not include long chain acyl-CoA synthetases from eukaryotic organisms.
authors.affiliation: Universität Hamburg, Institut für Allgemeine Botanik, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Fulda
authors.forename: E
authors.initials: E
authors.lastname: Heinz
authors.forename: F P
authors.initials: FP
authors.lastname: Wolter
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D006801:Humans; D008969:Molecular Sequence Data; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D000249:Adenosine Monophosphate; D003066:Coenzyme A Ligases; C085934:acyl-coenzyme A synthetase, E coli; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase