Interleukin-11 expression in donor bone marrow cells improves hematological reconstitution in lethally irradiated recipient mice.
Lethally irradiated mice were transplanted with syngeneic bone marrow cells infected with a recombinant retrovirus vector containing the human interleukin-11 (hIL-11) cDNA under the control of the human cytomegalovirus (CMV) immediate early promoter. The hIL-11 RNA transcript from the vector was detected in the spleen and bone marrow of the recipient mice, and hIL-11 protein accumulated in their serum. The hematological reconstitution of these mice was compared with recipient mice rescued with bone marrow infected with the parental retrovirus vector not containing the hIL-11 cDNA. The hIL-11-expressing mice had an accelerated recovery of circulating platelets and red and white blood cells. Three months after the transplantation, bone marrow was harvested from the mice and used to rescue other lethally irradiated recipients. The hIL-11 mRNA and protein were also detected in these secondary recipients, and the mice showed improved hematological reconstitution relative to a control group. No abnormal cell proliferation or other histopathology was observed in the hIL-11-expressing mice.
Paul
S R
SR
Genetics Institute, Cambridge, MA 02140.
Hayes
L L
LL
Palmer
R
R
Morris
G E
GE
Neben
T Y
TY
Loebelenz
J
J
Pedneault
G
G
Brooks
J
J
Blue
I
I
Moore
M A
MA
D000818:Animals; D001853:Bone Marrow / Q000737:chemistry* / Q000473:pathology* / Q000528:radiation effects*; D016026:Bone Marrow Transplantation / Q000473:pathology*; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005260:Female; D005822:Genetic Vectors; D006410:Hematopoiesis / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D017370:Interleukin-11 / Q000032:analysis* / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D013154:Spleen / Q000737:chemistry / Q000166:cytology; D014019:Tissue Donors; D014162:Transfection
D004279:DNA, Viral; D017370:Interleukin-11; D012333:RNA, Messenger