title: Taxoid side-chain structure determination by electrospray ionization tandem mass spectrometry.
abstract: A method to elucidate the side-chain structure of novel taxoids was developed through use of electrospray ionization tandem mass spectrometry. The intact side-chain fragments produced by source fragmentation in the atmosphere-to-vacuum interface were further dissociated in the collision cell of a triple quadrupole mass spectrometer. The distinctive collisionally induced dissociation mass spectra were remarkably informative and gave detailed information on the side-chain functionalities. This technique proved valuable in the characterization of new taxoids produced by yew-tree cell cultures.
authors.affiliation: Children's Hospital Oakland Research Institute, CA 94609.
authors.forename: F
authors.initials: F
authors.lastname: Bitsch
authors.forename: C H
authors.initials: CH
authors.lastname: Shackleton
authors.forename: W
authors.initials: W
authors.lastname: Ma
authors.forename: G
authors.initials: G
authors.lastname: Park
authors.forename: M
authors.initials: M
authors.lastname: Nieder
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry / Q000379:methods*; D017239:Paclitaxel / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D017239:Paclitaxel