Genetic mapping of tumor susceptibility genes involved in mouse plasmacytomagenesis.
Plasmacytomas (PCTs) were induced in 47% of BALB/cAnPt mice by the intraperitoneal injection of pristane, in 2% of (BALB/c x DBA/2N)F1, and in 11% of 773 BALB/cAnPt x (BALB/cAnPt x DBA/2N)F1 N2 backcross mice. This result indicates a multigenic mode of inheritance for PCT susceptibility. To locate genes controlling this complex genetic trait, tumor susceptibility in backcross progeny generated from BALB/c and DBA/2N (resistant) mice was correlated with alleles of 83 marker loci. The genotypes of the PCT-susceptible progeny displayed an excess homozygosity for BALB/c alleles within a 32-centimorgan stretch of mouse chromosome 4 (> 95% probability of linkage) with minimal recombination (12%) near Gt10. Another susceptibility gene on mouse chromosome 1 may be linked to Fcgr2 (90% probability of linkage); there were excess heterozygotes for Fcgr2 among the susceptible progeny and excess homozygotes among the resistant progeny. Regions of mouse chromosomes 4 and 1 that are correlated with PCT susceptibility share extensive linkage homology with regions of human chromosome 1 that have been associated with cytogenetic abnormalities in multiple myeloma and lymphoid, breast, and endocrine tumors.
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Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Krall
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MM
Dosik
J K
JK
D000818:Animals; D002874:Chromosome Mapping; D016147:Genes, Tumor Suppressor; D008040:Genetic Linkage; D005819:Genetic Markers; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D010954:Plasmacytoma / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D005819:Genetic Markers