[Various nuclear and cytoplasmic parameters in relation to the evolution of an in vitro culture of rat fibroblasts].
A cytokinetic study was performed on progressive subcultures of a cellular strain represented by fibroblasts (FG: Fibroblasts Galliera). Our previous studies on these cells showed morphofunctional changes in relation to cell ageing, indicating the stabilization of a cell line with high proliferative capacity. Furthermore, in the present work, we carried out a comparative analysis with a neoplastic strain (SGS/3A cells), characterized by a low state of cell differentiation. The morphological (fluorescence microscopy) and quantitative (flow cytometry) analysis, examined the relative DNA/protein and DNA/RNA ratios. Moreover, the replicative and mitotic activity and the apoptotic degeneration was determined. The results obtained indicated the existence in this biological model of a different relation between cell growth and cell proliferation. The analysis of our results allowed us to hypothesize that the cellular subpopulation selected during the culture progression of FG cells constitutes a cell line highly able of survival in vitro. In these cells the high proliferative activity is opposite to a reduced metabolism.
Sciola
L
L
Istituto di Fisiologia Generale e Chimica Biologica, Università di Sassari.
Spano
A
A
Pippia
P
P
Barni
S
S
D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D002455:Cell Division; D002467:Cell Nucleus; D048429:Cell Size; D016922:Cellular Senescence; D003593:Cytoplasm; D004247:DNA / Q000032:analysis; D005347:Fibroblasts / Q000737:chemistry / Q000648:ultrastructure*; D008055:Lipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D051381:Rats; D012513:Sarcoma, Experimental / Q000473:pathology; D012641:Selection, Genetic; D014407:Tumor Cells, Cultured
D008055:Lipids; D011506:Proteins; D004247:DNA
Studio di alcuni parametri nucleari e citoplasmatici in relazione all'evoluzione di una coltura in vitro di fibroblasti di ratto.