title: Hybridization to an anti-activated platelet monoclonal antibody enhances fibrinolytic potency on urokinase.
abstract: The B-chain of urokinase (UK) was covalently linked by disulfide bond to the Fab fragment of an anti-human activated platelet monoclonal antibody (SZ-51). The UK-SZ-51 conjugate retained the original binding specificity of its parent antibody, and produced about a 5-fold enhancement in clot lysis in plasma over that of the urokinase in vitro. Whereas UK significantly decreased the concentration of fibrinogen in plasma clot assay supernatants, UK-SZ-51 did not.
authors.affiliation: Department of Biochemistry, Nanjing University, PRC.
authors.forename: L
authors.initials: L
authors.lastname: Du
authors.forename: R R
authors.initials: RR
authors.lastname: Yu
authors.forename: Z C
authors.initials: ZC
authors.lastname: Hua
authors.forename: D X
authors.initials: DX
authors.lastname: Zhu
authors.forename: G X
authors.initials: GX
authors.lastname: Wu
authors.forename: C G
authors.initials: CG
authors.lastname: Ruan
mesh_terms: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D005342:Fibrinolysis / Q000187:drug effects*; D005343:Fibrinolytic Agents / Q000494:pharmacology; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D000922:Immunotoxins / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D005343:Fibrinolytic Agents; D007140:Immunoglobulin Fab Fragments; D000922:Immunotoxins; D014568:Urokinase-Type Plasminogen Activator