Determination of lomefloxacin in human plasma by solid-phase extraction and high-performance liquid chromatography with UV detection.
A high-performance liquid chromatographic method for the determination of lomefloxacin in human plasma has been developed and validated. A solid-phase extraction procedure was used to isolate lomefloxacin from the biological matrix prior to the quantitative analysis. The compound was separated on a Vydac anion-exchange column using acetonitrile-phosphate buffer (pH 7.0) as the mobile phase and quantified by measuring its UV absorbance at 280 nm. The lower limit of detection for the analyte was 0.05 micrograms ml-1. Enoxacin was used as the internal standard. The calibration graph of the method was linear from 0.1 to 10 micrograms ml-1 of lomefloxacin in human plasma. This procedure is suitable for pharmacological and pharmacokinetic studies of lomefloxacin.
Carlucci
G
G
Dipartimento di Chimica, Università dell'Aquila, Italy.
Cilli
A
A
Liberato
M
M
Mazzeo
P
P
D000890:Anti-Infective Agents / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D002021:Buffers; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003198:Computer Simulation; D015365:Enoxacin / Q000097:blood; D024841:Fluoroquinolones; D006801:Humans; D015363:Quinolones / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D002021:Buffers; D024841:Fluoroquinolones; D015363:Quinolones; D015365:Enoxacin; C053091:lomefloxacin