Transsynaptic expression of a presynaptic glutamate receptor during hippocampal long-term potentiation.
Repetitive activation of excitatory synapses in the hippocampus produces a persistent enhancement of synaptic efficiency known as long-term potentiation (LTP). In anesthetized and in freely moving rats, the induction of LTP in the perforant path led to a transient increase in the amount of messenger RNA (mRNA) coding for a presynaptic glutamate receptor (GR33) in dentate granule cells. The amount of GR33 mRNA was increased for at least 5 hours after the induction of LTP but was indistinguishable from control values 1 day after induction. The N-methyl-D-aspartate receptor antagonist 2-aminophosphonovalerate prevented the induction of both LTP and the increase in GR33 mRNA. The amount of GR33 protein was increased in the mossy fiber terminal zone of dentate granule cells 5 hours after the induction of LTP. These results suggest that the induction of LTP in synapses at one stage in a neural network may lead to modification in synaptic function at the next stage in the network.
Smirnova
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T
Laboratoire de génétique moléculaire de la neurotransmission et des processus neurodégénératifs, Centre National de la Recherche Scientifique (CNRS), Gif-sur-Yvette, France.
Laroche
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S
Errington
M L
ML
Hicks
A A
AA
Bliss
T V
TV
Mallet
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J
D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D005071:Evoked Potentials; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism* / Q000502:physiology; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000096:biosynthesis / Q000235:genetics*; D017661:Receptors, Presynaptic / Q000096:biosynthesis / Q000235:genetics*; D013569:Synapses / Q000378:metabolism*
D012333:RNA, Messenger; D017470:Receptors, Glutamate; D017661:Receptors, Presynaptic; D015763:2-Amino-5-phosphonovalerate