title: Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus.
abstract: The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV) strain Smith was determined and compared with the sequence of the gH of MCMV strain K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5.0 kb, which is synthesized late in infection. A recombinant vaccinia virus expressing the MCMV gH open reading frame was constructed (Vac-gH). Anti-MCMV serum precipitated a protein of 87K from Vac-gH-infected cells. Reactivity with a monoclonal antibody showed the identity of the MCMV gH with a 87K envelope glycoprotein described previously by Loh and Qualtiere. Immunization of mice with the Vac-gH recombinant gave rise to an anti-gH serum, which neutralized MCMV without complement in vitro.
authors.affiliation: Department of Virology, University of Ulm, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Rapp
authors.forename: P
authors.initials: P
authors.lastname: Lucin
authors.forename: M
authors.initials: M
authors.lastname: Messerle
authors.forename: L C
authors.initials: LC
authors.lastname: Loh
authors.forename: U H
authors.initials: UH
authors.lastname: Koszinowski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005814:Genes, Viral / Q000235:genetics; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007114:Immunization; D051379:Mice; D008969:Molecular Sequence Data; D018146:Muromegalovirus / Q000235:genetics* / Q000276:immunology; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D014616:Vaccinia virus / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
chemical_list: D006023:Glycoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins