Stimulation of truncated glucagon-like peptide-1 release from the isolated perfused canine ileum by glucose absorption.
To elucidate whether glucose absorption is necessary for oral glucose-induced truncated glucagon-like peptide-1 (tGLP-1) release, we examined tGLP-1 release by several stimulants from isolated perfused canine ileum. Glucose and nonabsorbable sugars, sucrose and 2-deoxyglucose (2DG), were intraluminally administrated. Following gel filtration of the perfusate after glucose ingestion, the main component of GLP-1 in the eluate is tGLP-1. Both glucose and tGLP-1 in the effluents increased after the intraluminal administration of 5% glucose, but not by that of sucrose or 2DG. Glucose absorption is more important than contact of glucose with ileal epithelium to stimulate tGLP-1 release.
Sugiyama
K
K
Third Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Manaka
H
H
Kato
T
T
Yamatani
K
K
Tominaga
M
M
Sasaki
H
H
D000818:Animals; D003847:Deoxyglucose / Q000378:metabolism / Q000494:pharmacology; D004285:Dogs; D005934:Glucagon / Q000378:metabolism*; D052216:Glucagon-Like Peptide 1; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D007082:Ileum / Q000378:metabolism*; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D010446:Peptide Fragments / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D013268:Stimulation, Chemical; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology
D010446:Peptide Fragments; D011498:Protein Precursors; D013395:Sucrose; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D003847:Deoxyglucose; D005947:Glucose