DNA stretching on functionalized gold surfaces.
We describe a method for anchoring bacteriophage lambda DNA by one end to gold by Au-biotin-streptavidin-biotin-DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA molecules of chromosome size to be stretched and aligned.
Zimmermann
R M
RM
Department of Molecular Biology, Princeton University, NJ 08544.
Cox
E C
EC
D010582:Bacteriophage lambda; D001483:Base Sequence; D001710:Biotin / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry*; D004574:Electromagnetic Fields; D006046:Gold / Q000737:chemistry*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data
D004279:DNA, Viral; D001710:Biotin; D006046:Gold