Monoclonal antibodies neutralizing the haemolytic activity of box jellyfish (Chironex fleckeri) tentacle extracts.
1. Three monoclonal antibodies have been produced which neutralize in vitro the haemolytic activity present in tentacle extracts of the box jellyfish (Chironex fleckeri). 2. Two of these monoclonal antibodies bound specifically to a component of relative molecular mass 50,000 in tentacle extract on Western blots. 3. This binding only occurred when the extracts were electrophoresed under non-reducing conditions. 4. The third monoclonal antibody did not display binding to Western blots of tentacle extract under any of our experimental conditions.
Collins
S P
SP
School of Chemical and Biological Sciences, Deakin University, Geelong, VIC, Australia.
Comis
A
A
Marshall
M
M
Hartwick
R F
RF
Howden
M E
ME
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001665:Binding Sites; D015153:Blotting, Western; D003064:Cnidarian Venoms / Q000276:immunology* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D006461:Hemolysis; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D009500:Neutralization Tests; D037401:Scyphozoa / Q000737:chemistry*; D014020:Tissue Extracts / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003064:Cnidarian Venoms; D014020:Tissue Extracts