Serotonin (5-HT) fibers of the rat dura mater: 5-HT-positive, but not authentic serotoninergic, tryptophan hydroxylase-like fibers.
Serotonin (5-HT)-positive, but not tryptophan-5-hydroxylase (TPOH)-positive, authentic serotoninergic fibers were shown in the rat dura mater. 5-HT immunoreactive fibers in the dura are postulated to result from 5-HT uptake from circulating blood elements (e.g. platelets, mast cells) by perivascular sympathetic nerve fibers. A robust TPOH-immunoreactive mast cell population was identified in the dura; this result confirms the TPOH antibody specificity to cells known to synthesize 5-HT. While these results indicate that there are no authentic serotoninergic fibers in the dura mater, the mast cells, platelets and cerebrospinal fluid can serve as a source of 5-HT activating 5-HT receptors known to be present in this tissue.
Stanley
M I
MI
Department of Neurosurgery, College of Medicine, University of Cincinnati, OH 45267-0515.
Berger
R J
RJ
Zuccarello
M
M
Keller
J T
JT
D000818:Animals; D000918:Antibody Specificity; D004299:Dopamine beta-Hydroxylase / Q000276:immunology / Q000378:metabolism; D004388:Dura Mater / Q000166:cytology* / Q000201:enzymology; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000201:enzymology; D009412:Nerve Fibers / Q000201:enzymology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000502:physiology*; D014365:Tryptophan Hydroxylase / Q000502:physiology*
D012701:Serotonin; D014365:Tryptophan Hydroxylase; D004299:Dopamine beta-Hydroxylase