Probability of mast cells to mediate anaphylaxis in skeletal muscle.
Are there enough mast cells in denervated skeletal muscle to account for autopharmacological mediation of the antigen potentials (APs) elicited by microtaps? Through rough qualitative estimations, some authors have suggested a positive answer to this question. However, in view of measurements performed in this investigation of both the density of mast cells and the diffusion coefficient of antigens, the probability of such mediated effects was found to be relatively low: P = 0.016 for egg albumin and P = 0.004 for ferritin. Therefore, most APs induced by microtaps should be attributed to the direct effect of antigen over the sensitized muscle fibers. Yet, both the density of mast cells found in this work and the known amount of histamine they are capable of releasing when challenged with antigen, support the hypothesis regarding the involvement of these cells when antigen is massively superfused so as to induce Schultz-Dale reactions in muscle strips. Under this circumstance, the direct and mediated mechanisms may coexist.
Sánchez-Mejorada
G
G
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México City, DF.
Merchant-Larios
H
H
Alonso-deFlorida
F
F
Morales
L B
LB
D000707:Anaphylaxis / Q000209:etiology*; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008407:Mast Cells / Q000276:immunology*; D008954:Models, Biological; D009121:Muscle Denervation; D009132:Muscles / Q000166:cytology / Q000276:immunology; D011336:Probability
D000941:Antigens