title: Neisseria gonorrhoeae possesses two nicotinamide adenine dinucleotide-independent lactate dehydrogenases.
abstract: An important metabolic capability of Neisseria gonorrhoeae is the utilization of host-derived lactate. Two isoenzymes of the membrane-associated, pyridine dinucleotide-independent type of lactate dehydrogenase (iLDH) participate in lactate assimilation, but exhibit distinctive properties. Isoenzyme iLDH-I utilized lactate exclusively as substrate, exhibiting a preference for the D-isomer. In contrast, isoenzyme iLDH-II exhibited broad substrate specificity (lactate, phenyllactate, and 4-hydroxyphenyllactate), but was stereospecific for the L-isomers. These results explain the difficulty in isolating mutants unable to utilize lactate.
authors.affiliation: Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
authors.forename: R S
authors.initials: RS
authors.lastname: Fischer
authors.forename: G C
authors.initials: GC
authors.lastname: Martin
authors.forename: P
authors.initials: P
authors.lastname: Rao
authors.forename: R A
authors.initials: RA
authors.lastname: Jensen
mesh_terms: D002462:Cell Membrane / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D009154:Mutation; D009243:NAD / Q000378:metabolism; D009344:Neisseria gonorrhoeae / Q000201:enzymology* / Q000472:pathogenicity; D013379:Substrate Specificity
chemical_list: D003470:Culture Media; D007527:Isoenzymes; D007773:Lactates; D009243:NAD; D007770:L-Lactate Dehydrogenase