Chromosomal organization of viral integration sites in human papillomavirus-immortalized human keratinocyte cell lines.
The target specificity of viral integration is essential to determining the biologic significance of this integration to various pathologic conditions, including cancer. In this study the chromosomal features of several human papillomavirus (HPV)-16 integration sites mapped by in situ hybridization in human keratinocyte lines were visualized directly by G-banding and differential labeling with bromodeoxyuridine of later replicating domains. G-negative chromosomal bands exhibiting late replication were selectively targeted by HPV-16, suggesting that the structural and functional relationship of the state of chromatin condensation and replication is critical in accessibility to virus integration.
Zimonjic
D B
DB
Laboratory of Biology, National Cancer Institute, Bethesda, MD 20892.
Popescu
N D
ND
DiPaolo
J A
JA
D001287:Attachment Sites, Microbiological; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D004261:DNA Replication; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000648:ultrastructure*; D027383:Papillomaviridae; D012854:Sister Chromatid Exchange; D016662:Virus Integration / Q000235:genetics*