title: Field testing an enzyme-linked synthetic oligonucleotide probe for identification of Anopheles gambiae s.s. and An. arabiensis.
abstract: Tests were carried out in Kenya to determine whether the enzyme-linked synthetic oligonucleotide probe (pAna 1) developed for identifying species of the Anopheles gambiae complex could be used under field conditions. The An. arabiensis male-specific pAna 1 probe was able to identify all male larval instars and adult males. However, the non-radioactive assay was not sufficiently sensitive to identify male sperm DNA in all the mated female An. arabiensis. Although the ratio of An. arabiensis to An. gambiae s.s. identified with pAna 1 in males during the dry season was in agreement with the ratio in half-gravid females identified cytogenetically, the ratios were different during the wet season. This study demonstrates that the enzyme-linked DNA probe assay is applicable under field conditions.
authors.affiliation: Kenya Medical Research Institute (KEMRI), Vector Biology and Control Research Centre, Kisumu, Kenya.
authors.forename: A K
authors.initials: AK
authors.lastname: Githeko
authors.forename: M W
authors.initials: MW
authors.lastname: Service
authors.forename: F K
authors.initials: FK
authors.lastname: Atieli
authors.forename: S M
authors.initials: SM
authors.lastname: Hill
authors.forename: J M
authors.initials: JM
authors.lastname: Crampton
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics; D004247:DNA / Q000032:analysis; D005260:Female; D007630:Kenya; D007814:Larva; D008297:Male; D015345:Oligonucleotide Probes; D012737:Sex Factors; D013094:Spermatozoa / Q000737:chemistry
chemical_list: D015345:Oligonucleotide Probes; D004247:DNA; D000469:Alkaline Phosphatase