Immunologic parameters in systemic sclerosis.
BACKGROUND
Immunologic abnormalities seem to play an important role in important role in systemic sclerosis (SSc).


METHODS
We studied the following immune parameters to get more insight into SSc: autoantibodies (antinuclear antibodies (ANA), anti-Scl-70, anticentromere antibodies (ACA) subsets of lymphocyte subpopulations and markers of their activation, as well as serum levels of IL-2, the soluble IL-2 receptor (SIL-2R), IL-6 and its correlation to N-terminal procollagen-III propeptide (P III P), and finally, the IL-6 production by SSc and normal dermal fibroblasts.


RESULTS
In patients with active SSc, we found a reduced number of CD2+ T-lymphocytes and an increase in the expression of T-lymphocyte activation markers such as CD25+ and CD71+, HLA-DR Ia, as well as elevated serum levels of SIL-2LR and IL-6. SSc fibroblasts did not produce more IL-6 than normal fibroblasts in monolayer cultures.


CONCLUSIONS
Our data show that a wide range of immunologic parameters are altered in SSc. In general, T-helper (TH) lymphocytes are activated possibly because of reduced T-suppressor (TS) and natural killer (NK)-cell levels. TH may polyclonally stimulate B cells, which in turn produce higher amounts of autoantibodies. Our findings support the concept that TH cell-derived cytokines/growth factors stimulate matrix protein synthesis by fibroblasts, resulting in generalized fibrosis.
Bruns
M
M
Department of Dermatology, University of Leipzig, Germany.
Herrmann
K
K
Haustein
U F
UF
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000974:Antibodies, Antinuclear / Q000032:analysis; D002503:Centromere / Q000276:immunology; D002648:Child; D004264:DNA Topoisomerases, Type I; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000276:immunology; D010446:Peptide Fragments / Q000032:analysis; D011347:Procollagen / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000032:analysis; D012595:Scleroderma, Systemic / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets
D000974:Antibodies, Antinuclear; D007376:Interleukin-2; D009687:Nuclear Proteins; D010446:Peptide Fragments; D011347:Procollagen; D015375:Receptors, Interleukin-2; C048100:Scl 70 antigen, human; C041876:procollagen Type III-N-terminal peptide; D004264:DNA Topoisomerases, Type I