Murine recombinant interleukin-3 induces recovery of T and B cells in irradiated mice.
Injection of murine recombinant interleukin-3 (IL-3) into (C57BL/10 x DBA/2)F1 mice, sublethally irradiated with 300 cGy and killed 14 days later, induced in the thymus recovery of the cell number and mitotic responsiveness to concanavalin A (Con A), as well as an increase in number of double-negative CD4-CD8-, double-positive CD4+ CD8+, and single-positive CD4+CD8- and CD4-CD8+ cells. Also in the spleen, the cell count and mitotic responsiveness to Con A and lipopolysaccharide were increased to normal levels by IL-3 treatment. If the assays were performed 21 or 28 days after irradiation, IL-3 treatment was able to restore thymus and spleen cell counts as well as T- and B-cell mitotic responsiveness, even when mice were exposed to 400 or 500 cGy, respectively. These results altogether indicate that IL-3 induces differentiation and growth of thymocytes and recovery of T- and B-cell functions in mice exposed to sublethal irradiation.
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Laboratory of Immunobiology, AMB-EFF, ENEA, Casaccia, Rome, Italy.
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Doria
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D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000528:radiation effects*; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002452:Cell Count; D005260:Female; D007377:Interleukin-3 / Q000494:pharmacology*; D016131:Lymphocyte Subsets; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000528:radiation effects*; D014916:Whole-Body Irradiation
D015704:CD4 Antigens; D016827:CD8 Antigens; D007377:Interleukin-3; D011994:Recombinant Proteins