Monoclonal antibody, LR-2, recognizes an immunoglobulin-linked signaling molecule on murine B cells.
The studies presented here examine the effect of a monoclonal rat antibody, LR-2, on the proliferation of growth-inhibitable murine B lymphoma lines. Like anti-immunoglobulin, mAb LR-2 is capable of inducing growth arrest in these cells, implying that ligation of the LR-2 cell surface molecule by mAb LR-2 transduces an inhibitory signal. The growth inhibition produced by LR-2 can, however, be clearly distinguished from that produced by anti-Ig reagents. Our findings show that mAb LR-2 does not react with membrane Ig. Significantly, the LR-2 antigen could be induced to comodulate with membrane Ig. The data thus indicate that mAb LR-2 reacts with a mIg-associated signal-transducing molecule on the B cell surface and may constitute a component of the Ig receptor complex. The role of this molecule in the regulation of the proliferation and maturation of normal B cells is currently being investigated.
Marshall-Clarke
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S
Department of Human Anatomy and Cell Biology, University of Liverpool, United Kingdom.
Greenwood
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M
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000276:immunology; D051379:Mice; D009154:Mutation; D051381:Rats; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D011947:Receptors, Antigen, B-Cell; D011971:Receptors, Immunologic