Partial sequence analysis of the hepatitis C viral genome in Singapore patients.
The distribution of hepatitis C viral (HCV) genotypes in Singapore has not been previously determined. We studied the sera of 40 Singapore patients which were PCR-positive for HCV. The HCV genotypes were determined by direct sequencing of amplified sequences of the 5' non-coding region, after reverse transcription. Of the 40 samples, 35/40 (87.5%) were of HCV type 1, 2/40 (5.0%) were of type 2 and 3/40 (7.5%) were of type 3. The most common HCV genotype in this study was the type 1 genotype. Our results confirm the wide geographical distribution of HCV genotypes.
Lee
A S
AS
Department of Clinical Research, Singapore General Hospital.
Ng
K Y
KY
Chee
M K
MK
Vathsala
A
A
Choong
H L
HL
Ng
H S
HS
Oon
C J
CJ
D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D006526:Hepatitis C / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D012846:Singapore
D012367:RNA, Viral