In vitro culture of preimplantation mouse embryos and day 12 limb-buds: effects of serum and albumin.
The effects of three different protein sources at different concentrations on the growth and development of preimplantation mouse embryos and day 12 mouse limb-buds in culture were studied. Mouse embryos and forelimb-buds were cultured with a range of concentrations (5.5 to 42%) of either donor bovine serum (DBS) or fetal bovine serum (FBS), or (0.2 to 0.8%) bovine serum albumin (BSA). After 48 h in culture, the rate of embryo development was significantly higher in 5.5% DBS than in all other groups (P < 0.05). The embryo hatching rate was higher in 21% FBS, 42% FBS, and all DBS groups than in serum-free medium, and all BSA groups (P < 0.05). Morphologic analysis of cultured limb-buds at 72 h revealed that total, paw, and cartilage area were greater (P < 0.05) in the serum-free medium than in all other groups. Shape factor analysis suggested that 5.5% DBS was most beneficial to mouse limb-bud development. No differences were seen in DNA or protein content of limb-buds among groups. Results suggest that mouse forelimb-buds can be successfully cultured in serum-free medium and that high concentrations of FBS and DBS may be detrimental for preimplantation embryo and/or limb-bud growth and development.
Tornesi
B
B
Department of Herd Medicine and Theriogenology, WCVM, University of Saskatchewan, Saskatoon, Canada.
Palasz
A T
AT
Del Campo
M R
MR
Rousseaux
C G
CG
Archer
F J
FJ
Mapletoft
R J
RJ
D000818:Animals; D002417:Cattle; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D005314:Embryonic and Fetal Development; D005121:Extremities / Q000196:embryology*; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012710:Serum Albumin, Bovine
D003470:Culture Media; D012710:Serum Albumin, Bovine