Polymerase chain reaction allelotyping of human ovarian cancer.
We have used a set of microsatellite polymorphisms (MSPs) to examine the location and frequency of allele loss throughout the genome in a panel of 25 human epithelial ovarian tumours. When more than one MSP was employed per arm, mean informativity was 85.2% (range 64-100%). The average fractional allelic loss was 0.28 (range 0-0.65). A high frequency of allele loss was seen at 5q (40%), 9q (48%), 11p (43%), 14q (46%), 15q (40%), 17p (61%), 17q (64%), 19p (45%) and Xp (40%), confirming previous findings at some sites, but also suggesting the existence of new tumour-suppressor genes in regions (9q, 14q, 15q) which have not previously been studied in ovarian cancer. For 9q and 14q, partial loss of the arm was more common than loss of heterozygosity for all loci. There was a significant relationship between allele loss affecting the short arm of chromosome 17 and allele loss affecting 17q (P < 0.001). No other relationship was detected between allele losses at different sites. Polymerase chain reaction allelotyping is suitable for the examination of very small tumour samples and tumours in which classical karyotyping is problematic.
Osborne
R J
RJ
Cancer Research Campaign Department of Clinical Oncology, University of Cambridge School of Clinical Medicine, Addenbrooke's Hospital, UK.
Leech
V
V
D000483:Alleles; D001483:Base Sequence; D002872:Chromosome Deletion; D002877:Chromosomes, Human; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D002888:Chromosomes, Human, Pair 19; D002895:Chromosomes, Human, Pair 5; D002899:Chromosomes, Human, Pair 9; D017931:DNA Primers; D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D014960:X Chromosome
D017931:DNA Primers; D004276:DNA, Satellite