title: Ovarial steroids and methionine-enkephalin modulation of adrenergic transmission in rabbit oviduct.
abstract: 1. The effect of methionine-enkephalin on the [3H]-noradrenaline ([3H]-NA) overflow and on contractions evoked by field electrical stimulation (FES) and by exogenous NA were studied in vitro in the isthmic part of oviduct of rabbits (untreated and treated with estradiol or progesterone). 2. The evoked tritium overflow (which reflected [3H]-NA overflow) was determined by liquid scintillation spectrometry. 3. Field electrical stimulation of 4 Hz (trains of 40 pulses, 0.3 msec) evoked guanethidine-sensitive contractions. 4. In all groups of animals methionine-enkephalin dose dependently decreased FES-evoked contractions but not those evoked by exogenous NA. 5. The amount of tritium overflow evoked by 4 Hz stimulation (600 pulses, 1 msec) was significantly lower in tissues from estradiol treated (1.16 +/- 0.19%) compared with those obtained in tissues from untreated (1.82 +/- 0.22%) and progesterone treated (2.07 +/- 0.21%) rabbits. Methionine-enkephalin, 1 microM, decreased the evoked tritium overflow in the isthmus from untreated rabbits by 36.1 +/- 3.6%, in estradiol treated by 22.8 +/- 2.9% and in progesterone treated by 52.3 +/- 4.5%. 6. The results suggest that the methionine-enkephalin effect on FES-evoked contractions could be due to a prejunctional effect on the adrenergic terminals and that there is a hormonal dependence of the opioid effect on [3H]-NA overflow.
authors.affiliation: Institute of Physiology, Bulgarian Academy of Sciences, Sofia.
authors.forename: L
authors.initials: L
authors.lastname: Chernaeva
authors.forename: Z
authors.initials: Z
authors.lastname: Mizhorkova
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D004744:Enkephalin, Methionine / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D005187:Fallopian Tubes / Q000294:innervation* / Q000502:physiology; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D006145:Guanethidine / Q000494:pharmacology; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009638:Norepinephrine / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D011817:Rabbits; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*
chemical_list: D012739:Gonadal Steroid Hormones; D011374:Progesterone; D004958:Estradiol; D004744:Enkephalin, Methionine; D009638:Norepinephrine; D006145:Guanethidine