Modulation of chemiluminescence in a murine macrophage cell line by neuroendocrine hormones.
This study determined the effects of neuropeptides and neuroendocrine hormones at the cellular level of the immune response using a murine macrophage cell line, J774, which exhibits a chemiluminescent oxidative burst upon acute stimulation with zymosan. We report that the zymosan-triggered oxidative burst of J774 cells can be modulated by the opioid peptides beta-endorphin (beta-END) and dynorphin A (DYN) in a naloxone-reversible fashion. Norepinephrine (NE) also modulated chemiluminescence (CL) emission of J774 cells, with dose-dependent suppression of CL dependent upon co-incubation with gamma-interferon (gamma-INF). Without gamma-INF co-incubation, NE shared with the opioid peptides beta-END and DYN the ability to modulate oxidative burst, producing an inverted-U dose response. These data indicate that J774 cells may be useful for explaining some mechanisms through which the neuroendocrine system interacts with the immune system.
Tosk
J M
JM
Psychiatry and Service, Jerry L. Pettis Memorial Veterans' Affairs Medical Center, Loma Linda, California 92357.
Grim
J R
JR
Kinback
K M
KM
Sale
E J
EJ
Bozzetti
L P
LP
Will
A D
AD
D000818:Animals; D002460:Cell Line; D004347:Drug Interactions; D004399:Dynorphins / Q000494:pharmacology; D004723:Endorphins / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009638:Norepinephrine / Q000494:pharmacology*; D011994:Recombinant Proteins; D016897:Respiratory Burst / Q000187:drug effects; D015054:Zymosan / Q000494:pharmacology; D001615:beta-Endorphin / Q000494:pharmacology
D004723:Endorphins; D011994:Recombinant Proteins; D001615:beta-Endorphin; D004399:Dynorphins; D007371:Interferon-gamma; D015054:Zymosan; D009638:Norepinephrine