Isolation of a mouse cDNA encoding Rab23, a small novel GTPase expressed predominantly in the brain.
The full-length cDNA encoding Rab23, a novel Ras-related small GTPase, was isolated using the sequence of a previously described [Chavrier et al., Gene 112 (1992) 261-264] short cDNA fragment and the rapid amplification of cDNA ends (RACE) PCR techniques. The deduced amino acid sequence was not very closely related to any previously described small GTPase, but was within the Rab subfamily. A Northern analysis revealed that the rab23 mRNA is predominantly expressed in the brain, which places the protein, together with Rab3a and Rab15, in the group of small GTPases characteristic of the nervous system.
Olkkonen
V M
VM
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Peterson
J R
JR
Dupree
P
P
Lütcke
A
A
Zerial
M
M
Simons
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology*; D003001:Cloning, Molecular / Q000379:methods; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000302:isolation & purification; D019204:GTP-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D020691:rab GTP-Binding Proteins
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D019204:GTP-Binding Proteins; C431746:Rab23 protein, mouse; D020691:rab GTP-Binding Proteins