title: Single-channel and whole-cell recordings from on-neurone glial cells in Helix pomatia ganglia.
abstract: A procedure is described for performing patch-clamp recordings on satellite glial cells kept in place within the nervous ganglia in the mollusc Helix. Glial cell properties were deduced from whole-cell and cell-attached recordings. The glial membrane was found to contain densely packed inwardly rectifying K+ channels. Activation of the neurones, under either current-clamp or voltage-clamp conditions, depolarized the glial cell layer wrapped around the neurones and induced a delayed persistent increase in the K+ channel opening probability. These results suggest that the glial channels opened in response to a signal emanating from the active neurones. This preparation provides a useful means of detecting and analysing neurone-glial interactions at the cell and unitary channel levels.
authors.affiliation: Laboratoire de Neurobiologie, CNRS, Marseille, France.
authors.forename: I
authors.initials: I
authors.lastname: Gommerat
authors.forename: G
authors.initials: G
authors.lastname: Jacquet
authors.forename: H
authors.initials: H
authors.lastname: Chagneux
authors.forename: M
authors.initials: M
authors.lastname: Gola
mesh_terms: D000818:Animals; D002450:Cell Communication; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D005724:Ganglia / Q000502:physiology*; D006372:Helix, Snails; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D008564:Membrane Potentials; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009457:Neuroglia / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D015221:Potassium Channels / Q000502:physiology*
chemical_list: D015221:Potassium Channels