[Studies on the strain differences of Schistosoma japonicum in the mainland of China. XII. DNA hybridization of five isolates].
The nonradioactive labelled probe pSM 889 was hybridized to Southern blots of genomic DNA of five isolates of Schistosoma japonicum from Anhui, Hubei, Guangxi, Sichuan and Yunnan in the mainland of China. The major bands of hybridization of DNA digested by restriction endonucleases EcoRI and BamHI are same among the five isolates, but the minor bands of hybridization of DNA digested by EcoRI show some differences between 4.4-9.6 kb and between 2.3-4.4 kb among the five isolates. The result shows that the five isolates are of some genetic variation. It provides, at molecular level, evidence of the existence of different strains of S. japonicum in the mainland of China.
Xie
M
M
Institute of Parasitic Disease, Chinese Academy of Preventive Medicine, Shanghai.
He
Y X
YX
Qiu
L S
LS
Chen
W
W
D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D016054:DNA, Protozoan; D016678:Genome; D008297:Male; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012549:Schistosoma japonicum / Q000145:classification* / Q000235:genetics*; D013045:Species Specificity
D015342:DNA Probes; D016054:DNA, Protozoan; D004247:DNA