title: Members of the low density lipoprotein receptor family mediate cell entry of a minor-group common cold virus.
abstract: A protein binding to a minor-group human rhinovirus (HRV2) was purified from HeLa cell culture supernatant. The amino acid sequences of tryptic peptides showed identity with the human low density lipoprotein (LDL) receptor (LDLR). LDL and HRV2 mutually competed for binding sites on human fibroblasts. Cells down-regulated for LDLR expression yielded much less HRV2 upon infection than cells with up-regulated LDLR. Virus also bound to the large subunit of the alpha 2-macroglobulin receptor/LDLR-related protein (alpha 2MR/LRP). LDLR-deficient fibroblasts yielded considerably less virus in the presence of receptor-associated protein (RAP), providing evidence that alpha 2MR/LRP also acts as a minor group HRV receptor.
authors.affiliation: Institute of Biochemistry, Medical Faculty, University of Vienna, Austria.
authors.forename: F
authors.initials: F
authors.lastname: Hofer
authors.forename: M
authors.initials: M
authors.lastname: Gruenberger
authors.forename: H
authors.initials: H
authors.lastname: Kowalski
authors.forename: H
authors.initials: H
authors.lastname: Machat
authors.forename: M
authors.initials: M
authors.lastname: Huettinger
authors.forename: E
authors.initials: E
authors.lastname: Kuechler
authors.forename: D
authors.initials: D
authors.lastname: Blaas
mesh_terms: D000595:Amino Acid Sequence; D001667:Binding, Competitive; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology; D006367:HeLa Cells; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000378:metabolism / Q000382:microbiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D011973:Receptors, LDL / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D012229:Rhinovirus / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D011973:Receptors, LDL; D011991:Receptors, Virus