Structure and expression of the gene for the prothoracicotropic hormone of the silkmoth Bombyx mori.
We cloned and characterized two allelic variants of the gene for the Bombyx mori prothoracicotropic hormone (PTTH), a homodimeric 30-kDa brain secretory protein. These PTTH genes contain five exons that encode a precursor protein consisting of 224 amino acid residues whose C-terminal 109 residues represent the PTTH subunit. The Bombyx haploid genome contains a single copy of the PTTH gene. The major site of PTTH expression is the brain but expression at a very low level occurs in the gut. One Bombyx brain at day 0 of the fifth larval instar contained 2.4-2.8 pg PTTH mRNA, and this amount did not change markedly during larval-pupal development.
Adachi-Yamada
T
T
Department of Biology, School of Science, Nagoya University, Japan.
Iwami
M
M
Kataoka
H
H
Suzuki
A
A
Ishizaki
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D012831:Bombyx / Q000235:genetics*; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D017931:DNA Primers; D015870:Gene Expression; D017344:Genes, Insect; D015698:Genomic Library; D007301:Insect Hormones / Q000096:biosynthesis / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic
D017931:DNA Primers; D007301:Insect Hormones; D046911:Macromolecular Substances; D009479:Neuropeptides; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; C009089:prothoracicotropic hormone; D004247:DNA