title: Essential role of myosin S-2 region in muscle contraction.
abstract: We studied the contraction characteristics and Mg-ATPase activity of glycerinated rabbit psoas muscle fibers in the presence and absence of polyclonal antibody directed against the subfragment-2 (S-2) region of myosin, to give information about the role of myosin hinge region in muscle contraction. The antibody was kindly supplied to us from Professor Harrington's laboratory. The antibody-induced decrease of Ca(2+)-activated isometric force development was always accompanied by a parallel decrease of muscle fiber stiffness, so that the stiffness versus force relation remained the same by the antibody treatment. Force-velocity curves, obtained by applying ramp decreases in load from steady isometric force to zero, indicated that the antibody had no effect on the maximum shortening velocity and the shape of the force-velocity curve. Simultaneous measurements of Mg-ATPase activity and Ca(2+)-activated isometric force showed that Mg-ATPase activity of the fibers remained unchanged despite the antibody-induced decrease of isometric force even to zero. These results indicate that, if the antibody attaches to the S-2 region of myosin molecules, their heads still hydrolyze ATP without contributing to both muscle force generation and muscle fiber stiffness.
authors.affiliation: Department of Physiology, School of Medicine, Teikyo University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Noguchi
authors.forename: T
authors.initials: T
authors.lastname: Gross
authors.forename: H
authors.initials: H
authors.lastname: Sugi
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D007537:Isometric Contraction / Q000276:immunology; D009119:Muscle Contraction / Q000276:immunology / Q000502:physiology*; D015879:Myosin Subfragments / Q000276:immunology / Q000502:physiology*; D016658:Psoas Muscles / Q000502:physiology; D011817:Rabbits
chemical_list: D000906:Antibodies; D015879:Myosin Subfragments; D002118:Calcium