title: Gingival fluid IL-1 beta and IL-6 levels in menopause.
abstract: Menopause and oophorectomy without estrogen therapy (ED) have been associated with increased production of bone-active cytokines by peripheral blood mononuclear cells. The current study extended evaluation to gingival crevicular fluid (GCF) levels of interleukin (IL)-1 beta and IL-6 in such subjects compared to premenopausal and postmenopausal estrogen-treated females (ES). 13 ED and 13 ES Caucasians with a history of moderate-severe adult periodontitis provided GCF from 1-3 clinically identical sites each (5-6 mm probing depth, 5-7 mm clinical attachment loss, bleeding on probing). 30 s GCF samples were obtained and evaluated for IL-1 beta and IL-6 levels using two-site enzyme-linked immunosorbent assays (ELISAs). The frequency of GCF IL-1 beta-positive subjects was elevated in ED versus ES (92% versus 23%; p < 0.0004, chi 2 analysis). IL-6 was detected more frequently in ED subjects (23% versus 8%; not significant); however, the frequency of IL-6 detection was low in both groups due to short sampling times. These data support the concept that clinical conditions causing low estrogen environments allow increased local production of the bone-active cytokine IL-1 beta, and perhaps IL-6.
authors.affiliation: Department of Surgical Specialties, University of Nebraska Medical Center, Lincoln.
authors.forename: R A
authors.initials: RA
authors.lastname: Reinhardt
authors.forename: M P
authors.initials: MP
authors.lastname: Masada
authors.forename: J B
authors.initials: JB
authors.lastname: Payne
authors.forename: A C
authors.initials: AC
authors.lastname: Allison
authors.forename: L M
authors.initials: LM
authors.lastname: DuBois
mesh_terms: D000328:Adult; D000367:Age Factors; D001862:Bone Resorption / Q000378:metabolism; D015914:Estrogen Replacement Therapy; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000276:immunology; D005884:Gingival Hemorrhage / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D015850:Interleukin-6 / Q000032:analysis*; D008593:Menopause / Q000276:immunology / Q000378:metabolism*; D008875:Middle Aged; D010052:Ovariectomy; D017622:Periodontal Attachment Loss / Q000378:metabolism; D010514:Periodontal Pocket / Q000378:metabolism; D010518:Periodontitis / Q000276:immunology / Q000378:metabolism*; D017697:Premenopause / Q000276:immunology / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6