title: PCR analysis of a three-allelic PvuII--RFLP at D4S127 closely linked to the Huntington disease locus.
abstract: The polymorphic probe BJ56PS18 defined the genetic locus D4S127 closely linked to the Huntington disease (HD) gene. BJ56PS18 detects a three-allelic polymorphism after PvuII digestion in Southern blots. For rapid genotyping in HD-families, two different PCR conditions were established.
authors.affiliation: Institut für Humangenetik der Universität Göttingen, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Willoh
authors.forename: C
authors.initials: C
authors.lastname: Zühlke
authors.forename: B
authors.initials: B
authors.lastname: Gerdes
authors.forename: S
authors.initials: S
authors.lastname: Wiese
authors.forename: U
authors.initials: U
authors.lastname: Thies
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D008040:Genetic Linkage; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length