Establishment of a human myeloma cell line with growth-promoting activity for bone marrow-derived fibroblastoid colony-forming cells.
A human myeloma cell line, PCM6, was newly established from peripheral blood of a patient with advanced IgG myeloma by addition of recombinant interleukin-6 (IL-6) in culture. PCM6 cells had a morphology typical of mature plasma cells. Cytogenetic and surface marker studies confirmed that PCM6 cells were identical to fresh myeloma cells. Coculture of PCM6 cells with normal bone marrow mononuclear cells resulted in increased colony size of bone marrow-derived fibroblastoid colony-forming cells (CFU-F). Conditioned medium of PCM6 (PCM6-CM) cells increased the CFU-F colony size in a dose-dependent manner. The activity was labile to trypsin treatment but was heat stable (60 degrees C, 30 minutes). Molecular weight of the activity was approximately 165 kd by Sephacryl S-300 gel filtration. Platelet-derived growth factor (PDGF), epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta), and IL-1 beta were not detectable in the conditioned medium. These findings suggest that in some myeloma cases, bone marrow stroma may be affected by CFU-F growth-promoting activity.
Takahira
H
H
Department of Hematology, National Kyushu Cancer Center Hospital, Fukuoka, Japan.
Kozuru
M
M
Hirata
J
J
Obama
K
K
Uike
N
N
Iguchi
H
H
Miyamura
T
T
Yamashita
S
S
Kono
A
A
Umemura
T
T
D000954:Antigens, Surface / Q000032:analysis; D001483:Base Sequence; D001853:Bone Marrow / Q000473:pathology*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D017077:Culture Media, Conditioned / Q000032:analysis / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000473:pathology*; D016679:Genome, Viral; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007375:Interleukin-1 / Q000032:analysis; D015850:Interleukin-6 / Q000494:pharmacology; D008875:Middle Aged; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000276:immunology / Q000473:pathology*; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D017077:Culture Media, Conditioned; D007074:Immunoglobulin G; D007375:Interleukin-1; D015850:Interleukin-6; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D014357:Trypsin