title: Ehrlichia chaffeensis expresses an immunoreactive protein homologous to the Escherichia coli GroEL protein.
abstract: A clone expressing a 58-kDa protein reactive with human convalescent-phase serum was isolated from a recombinant library of Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. Sequencing identified two open reading frames, one encoding a 10.3-kDa polypeptide consisting of 94 amino acids and another encoding a 58-kDa polypeptide consisting of 550 amino acids. The sequences of the 10.3- and 58-kDa polypeptides were homologous to those of the Escherichia coli GroES and GroEL heat shock proteins, respectively.
authors.affiliation: Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
authors.forename: J W
authors.initials: JW
authors.lastname: Sumner
authors.forename: K G
authors.initials: KG
authors.lastname: Sims
authors.forename: D C
authors.initials: DC
authors.lastname: Jones
authors.forename: B E
authors.initials: BE
authors.lastname: Anderson
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D018835:Chaperonin 10; D018834:Chaperonin 60; D004536:Ehrlichia / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D006360:Heat-Shock Proteins