Human GATA-3 trans-activation, DNA-binding, and nuclear localization activities are organized into distinct structural domains.
GATA-3 is a zinc finger transcription factor which is expressed in a highly restricted and strongly conserved tissue distribution pattern in vertebrate organisms, specifically, in a subset of hematopoietic cells, in cells within the central and peripheral nervous systems, in the kidney, and in placental trophoblasts. Tissue-specific cellular genes regulated by GATA-3 have been identified in T lymphocytes and the placenta, while GATA-3-regulated genes in the nervous system and kidney have not yet been defined. We prepared monoclonal antibodies with which we could dissect the biochemical and functional properties of human GATA-3. The results of these experiments show some anticipated phenotypes, for example, the definition of discrete domains required for specific DNA-binding site recognition (amino acids 303 to 348) and trans activation (amino acids 30 to 74). The signaling sequence for nuclear localization of human GATA-3 is a property conferred by sequences within and surrounding the amino finger (amino acids 249 to 311) of the protein, thereby assigning a function to this domain and thus explaining the curious observation that this zinc finger is dispensable for DNA binding by the GATA family of transcription factors.
Yang
Z
Z
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500.
Gu
L
L
Romeo
P H
PH
Bories
D
D
Motohashi
H
H
Yamamoto
M
M
Engel
J D
JD
D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002451:Cell Compartmentation; D002460:Cell Line; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000648:ultrastructure*; D050990:GATA3 Transcription Factor; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000648:ultrastructure*; D011993:Recombinant Fusion Proteins; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000648:ultrastructure*; D014158:Transcription, Genetic; D016335:Zinc Fingers
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators