title: Calcitonin releases acid phosphatase from rat ventral prostate explants.
abstract: Inclusion of salmon calcitonin in the culture medium of rat ventral prostate explants diminished l-tartarate-sensitive acid phosphatase activity in the tissues with a concomitant increment of the enzyme activity in the medium. The effect of the hormone was dose-dependent for a dose range of 10(-12)-10(-6) M. Acid phosphatase activity in prostate explants decreased from 38.6 +/- 3.5 to 20.5 +/- 2.8, whereas it increased from 0.60 +/- 0.15 to 2.80 +/- 0.40 nmol p-nitrophenol liberated/mg protein/30 min in the culture medium. Tissues exposed to 10(-6) M salmon calcitonin had higher acetylcholinesterase activity (8.8 +/- 0.7) than non-exposed ones (6.2 +/- 0.5 mumol substrate hydrolyzed/g tissue/min). These results suggest that locally produced calcitonin causes a release for prostatic acid phosphatase from prostate tissues possibly through its interaction with the cholinergic system.
authors.affiliation: Department of Biochemistry, Cairo University, Giza, Egypt.
authors.forename: A
authors.initials: A
authors.lastname: Latif
authors.forename: A M
authors.initials: AM
authors.lastname: Nakhla
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D002116:Calcitonin / Q000494:pharmacology*; D003470:Culture Media; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D008297:Male; D011467:Prostate / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003470:Culture Media; C028815:salmon calcitonin; D002116:Calcitonin; D000110:Acetylcholinesterase; D000135:Acid Phosphatase