title: Chemoenzymic preparation of a glycoconjugate polymer having a sialyloligosaccharide: Neu5Ac alpha (2-->3)Gal beta (1-->4)GlcNAc.
abstract: Water-soluble polyacrylamide having 3'-sialyl N-acetyl-lactosamine [Neu5Ac alpha (2-->3)Gal beta (1-->4)GlcNAc] was enzymatically prepared by stepwise sugar-elongation on a water-soluble GlcNAc-bearing polyacrylamide. It was demonstrated that the flexible GlcNAc branches of the polymer chains allow quantitative galactosylation with bovine galactosyl transferase and partial sialylation by Trypanosoma cruzi trans-sialidase. Unsialylated N-acetyl-lactosamine side chains can be removed with beta-D-galactosidase and N-acetyl-beta-D-glucosaminidase to afford the targeted polymer containing 3'-sialyl N-acetyl-lactosamine.
authors.affiliation: Division of Biological Science, Graduate School of Science Hokkaido University, Sapporo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nishimura
authors.forename: K B
authors.initials: KB
authors.lastname: Lee
authors.forename: K
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Y C
authors.initials: YC
authors.lastname: Lee
mesh_terms: D000116:Acetylgalactosamine / Q000737:chemistry; D002240:Carbohydrate Sequence; D006001:Glycoconjugates / Q000138:chemical synthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D012794:Sialic Acids / Q000737:chemistry
chemical_list: D006001:Glycoconjugates; D009844:Oligosaccharides; D012794:Sialic Acids; D000116:Acetylgalactosamine