[The effect of arachidonic acid and hydrocortisone on the intracellular Ca2+ concentration in murine plasmacytoma JW cells].
Arachidonic acid (1-3 microM) induces a rapid dose-dependent rise of free Ca2+ contents [Ca2+]i in the cytoplasm of JW cells. An increase of [Ca2+]i is the consequence of Ca2+ influx and Ca2+ efflux from intracellular calcium sources. Hydrocortisone (0.5-5 microM) blocks plasma membrane Ca2+ channels and inhibits arachidonic acid-stimulated Ca(2+) influx in JW cells. The presented data suggest that arachidonic acid and hydrocortisone have membranotropic activity on plasmacytoma JW cells.
Astashkin
E I
EI
Khodorova
A B
AB
Tumanova
O A
OA
Samoĭlova
V E
VE
Dukhanin
A S
AS
D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D006854:Hydrocortisone / Q000494:pharmacology*; D051379:Mice; D010954:Plasmacytoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D005456:Fluorescent Dyes; C049925:fura-2-am; D016718:Arachidonic Acid; D002118:Calcium; D016257:Fura-2; D006854:Hydrocortisone
Vliianie arakhidonovoĭ kisloty i gidrokortizona na vnutrikletochnuiu kontsentratsiiu Ca2+ v plazmatsitomnykh JW-kletkakh mysheĭ.