Alterations in 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) mRNA accumulation induced by beta-adrenergic stimulation in cultured porcine granulosa cells.
1. In primary cultures of porcine granulosa cells incubation with isoproterenol (10(-7)-10(-5) M) produced a dose-dependent increase in 3 beta-HSD mRNA, 3 beta-HSD content and progesterone production which ranged from 1.5- to 5-fold. 2. These effects were completely blocked by alprenolol. Terbutaline (10(-6) M) increased 3 beta-HSD mRNA, 3 beta-HSD content and progesterone production (1.5- to 3-fold), an effect which could be prevented by alprenolol. 3. Treatment with dobutamine (10(-6) M) produced no significant change in 3 beta-HSD mRNA accumulation. 4. The results suggest that beta-adrenergic agonists have the capacity to regulate transcription and translation of 3 beta-HSD mRNA in granulosa cells and this effect is mediated by the beta 2 adrenergic receptor.
Li
X M
XM
Neuropsychiatric Research Unit, University of Saskatchewan, Saskatoon, Canada.
Juorio
A V
AV
Chedrese
P J
PJ
Murphy
B D
BD
D015096:3-Hydroxysteroid Dehydrogenases / Q000096:biosynthesis* / Q000235:genetics; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000526:Alprenolol / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D015342:DNA Probes; D004280:Dobutamine / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D011374:Progesterone / Q000096:biosynthesis; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D013552:Swine; D013726:Terbutaline / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D000318:Adrenergic beta-Agonists; D015342:DNA Probes; D012333:RNA, Messenger; D004280:Dobutamine; D011374:Progesterone; D000526:Alprenolol; D015096:3-Hydroxysteroid Dehydrogenases; D007545:Isoproterenol; D013726:Terbutaline