Cross-hybridization of equid herpesvirus-2 (EHV-2) and herpes simplex virus-1 (HSV-1) genes to equid herpesvirus-1 (EHV-1).
In contrast to previous findings, the Ab4 isolate of equid herpesvirus-1 (EHV-1) was shown to share homology with the G9 isolate of equid herpesvirus-2 (EHV-2). Using Southern blotting and stringent hybridization conditions, a significant proportion of this cross-hybridization was identified by the immediate-early gene-3 (IE-3) probe from herpes simplex virus-1 (HSV-1). The HSV-1 UL48 gene probe (encoding the IE gene transactivating protein VmW65, which is also known as alpha-TIF or VP16) was used to identify and isolate its counterpart in EHV-1. The relevance of shared homology to transactivation is being investigated.
Purewal
A S
AS
Department of Veterinary Pathology, Royal Veterinary College, London, UK.
Smallwood
A V
AV
Allsopp
R
R
Welch
H M
HM
Edington
N
N
D015139:Blotting, Southern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D018139:Simplexvirus / Q000235:genetics*
D015342:DNA Probes; D004279:DNA, Viral