[Genotypic characteristics of Rickettsia sibirica strains].
Six Rickettsia sibirica strains isolated in Siberia and Far East (Primorje) from various sources (patient, ticks D. nuttali, D. silvarum, H. concinna) at different time (1940-1980) were studied by the RFLP and DNA probe hybridization techniques. All studied strains were found to have the identical profiles of migrating fragments in restrictograms got by using a set of endonucleases (EcoRI, PstI, PvuII, Bg1I, XbaI, HindIII, MspI) and similar zones of hybridization with a DNA probe derived from Rickettsia prowazekii DNA. The obtained data point to a close similarity between the genomes of investigated Rickettsia sibirica strains. Long-term isolation of the genetically similar Rickettsia sibirica strains testifies to their constant circulation, thus apparently determining the stability of epidemiologic manifestation of tick-borne typhus fever of Northern Asia in the central part of its area (Siberia, Far East).
Balaeva
N M
NM
Artem'ev
M I
MI
Ignatovich
V F
VF
Likhoded
L Ia
LIa
Genig
V A
VA
Demkin
V V
VV
Rydkina
E B
EB
Rudakov
N V
NV
Reshetnikova
T A
TA
Samoĭlenko
I E
IE
D015342:DNA Probes; D005798:Genes, Bacterial; D005838:Genotype; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012281:Rickettsia / Q000235:genetics*; D013045:Species Specificity
D015342:DNA Probes
Genotipicheskaia kharakteristika shtammov Rickettsia sibirica.