[A study on changes of adrenergic receptors and their clinical significance in the lung of rats with respiratory distress syndrome].
The Bmax of value of beta receptors in membrane prepared from lung tissue of ARDS rats were remarkably decreased (P < 0.01) after 4h of injury (307 +/- 55fmol/mg protein) and 6h (240 +/- 66fmol/mg protein) as compared with that of control (490 +/- 61fmol/mg protein), whereas that of the alpha 1 receptors was increased markedly (P < 0.01) after 4h of injury (127 +/- 12fmol/mg protein) as compared with that of the control (83 +/- 7fmol/mg protein). In anisodamine treatment group, the Bmax of beta receptor (529 +/- 113) and content of cAMP was higher significantly (P < 0.01) than that of the untreated group. The activity of PLA2 in the lung tissue is remarkably lower (146 +/- 25U) than that of untreated group (P < 0.01). The present study showed the first time the imbalance between alpha and beta adrenoceptors in the lung of rat with ARDS, and confirmed that anisodamine has a role of regulation of beta receptor, cAMP and PLA2 in the lung of RDS rat.
Liu
Y M
YM
Department of Respiratory Medicine, General Hospital of Beijing Military Command.
D000317:Adrenergic alpha-Antagonists / Q000627:therapeutic use; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D000242:Cyclic AMP / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D009829:Oleic Acids; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D012991:Solanaceous Alkaloids / Q000627:therapeutic use*
D000317:Adrenergic alpha-Antagonists; D000894:Anti-Inflammatory Agents, Non-Steroidal; D009829:Oleic Acids; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D012991:Solanaceous Alkaloids; C003922:anisodamine; D000242:Cyclic AMP; D010741:Phospholipases A; D054467:Phospholipases A2