CGRP immunoreactive neurons in rat dorsal root ganglia do not all contain low-affinity NGF receptor immunoreactivity.
Nerve growth factor (NGF) and its homologues bind to the low affinity NGF receptor (LNGFR). We have determined whether the LNGFR colocalizes with calcitonin gene-related peptide (CGRP) as this peptide is restricted to neurons exhibiting high affinity binding sites for NGF. LNGFR and CGRP immunoreactivities were detected in 57% and 49% of spinal ganglion neurons respectively with different soma areas and only partial colocalization. Our data suggest that the LNGFR may not be essential for a functional neurotrophin response.
Zhou
X F
XF
Department of Physiology, Flinders University of South Australia, Bedford Park.
Gai
W P
WP
Rush
R A
RA
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D005727:Ganglia, Spinal / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons / Q000276:immunology / Q000378:metabolism*; D009475:Neurons, Afferent / Q000276:immunology / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000096:biosynthesis; D051381:Rats; D011921:Rats, Inbred WKY; D017475:Receptors, Nerve Growth Factor / Q000276:immunology / Q000378:metabolism*
D018377:Neurotransmitter Agents; D017475:Receptors, Nerve Growth Factor; D015740:Calcitonin Gene-Related Peptide