Uptake, subcellular distribution and metabolism of the phospholipid analogue hexadecylphosphocholine in MDCK cells.
The uptake, subcellular distribution and metabolism of hexadecylphosphocholine was investigated using hexadecylphospho-[methyl-14C]choline as tracer. The phospholipid analogue was taken up in a time-, dose- and temperature-dependent manner and showed a high affinity for the plasma membrane in vivo and in vitro. After incorporation into the plasma membrane, hexadecylphosphocholine appeared in all subcellular membranes investigated but not in the cytosolic fraction. After 24 h, the detectable metabolites of incorporated hexadecylphosphocholine co-chromatographed with the phosphatidylcholine standard (34.3%), the phosphocholine standard (22.1%), the glycerophosphocholine standard (4.3%) and the betaine standard (3.6%).
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Institut für Molekularbiologie und Biochemie, Freien Universität Berlin, Germany.
Wieder
T
T
Haase
A
A
Reutter
W
W
Morré
D M
DM
Morré
D J
DJ
D000818:Animals; D002250:Carbon Radioisotopes; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D010713:Phosphatidylcholines / Q000032:analysis / Q000096:biosynthesis*; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism* / Q000648:ultrastructure; D013696:Temperature; D013997:Time Factors
D002250:Carbon Radioisotopes; D010713:Phosphatidylcholines; D010767:Phosphorylcholine; C039128:miltefosine