title: Generation of selenium-containing abzyme by using chemical mutation.
abstract: A new strategy for generating abzyme was developed. Glutathione peroxidase (GPX, EC 1.11.1.9) is one of the important members of antioxidation enzyme system; it catalyzes the reductions of a variety of hydroperoxides in presence of glutathione(GSH). We have first prepared the monoclonal antibody (McAb) with GSH binding sites, then incorporated GPX catalytic group selenocystein (SeCys) into the antibody combining sites by using chemical mutation. Thus the mutated antibody displays high GPX activity, which approaches the magnitude level of native GPX, exhibits the kinetic behavior similar to native GPX, and has some advantages over native GPX.
authors.affiliation: National Laboratory of Enzyme Engineering, Jilin University, Changchun, P.R. China.
authors.forename: G M
authors.initials: GM
authors.lastname: Luo
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Zhu
authors.forename: L
authors.initials: L
authors.lastname: Ding
authors.forename: G
authors.initials: G
authors.lastname: Gao
authors.forename: Q A
authors.initials: QA
authors.lastname: Sun
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: T S
authors.initials: TS
authors.lastname: Yang
authors.forename: J C
authors.initials: JC
authors.lastname: Shen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay; D005978:Glutathione / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D006241:Haptens; D007074:Immunoglobulin G / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D016296:Mutagenesis; D011817:Rabbits / Q000276:immunology; D012643:Selenium; D017279:Selenocysteine / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D007074:Immunoglobulin G; D017279:Selenocysteine; C030190:S-(2,4-dinitrophenyl)glutathione; D005979:Glutathione Peroxidase; D005978:Glutathione; D012643:Selenium