Light and heavy chains specifying a human IgM kappa autoantibody to a T-cell receptor V beta-antigen.
Humans frequently produce serum IgM autoantibodies reactive with T-cell receptor beta chains at a determinant defined by peptides corresponding to the first complementarity determining region. It is likely that this determinant serves as a public idiotype involved in immunoregulation. Following screening of culture supernatants from over 60 Epstein-Barr virus-carrying B-cell lines of normal and neoplastic origin, we identified a line, IARC307, that secretes an IgM kappa protein showing marked specificity for the V beta 8.1 CDR1 sequence CKPISGHNSLFQWYRQT. We cloned and sequenced the complete variable regions of the V kappa and VH chains used by the autoantibody. The light chain has a V kappa III sequence related to the 'a' subgroup and uses J kappa 2. The heavy chain has a VHIII sequence essentially identical to the germ-line sequence DP54 and uses the JH6C minigene. The CDR3 is unique, differing from those of other autoantibodies. The antibody is rigorously specific in its specificity for the V beta 8 peptide and does not show polyspecificity for protein or DNA antigens.
Dedeoglu
F
F
University of Arizona, College of Medicine, Tucson 85724.
Kaymaz
H
H
Klein
G
G
Marchalonis
J J
JJ
D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000737:chemistry*; D001483:Base Sequence; D018076:DNA, Complementary / Q000096:biosynthesis; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry*; D007075:Immunoglobulin M / Q000737:chemistry*; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains / Q000737:chemistry*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*
D001323:Autoantibodies; D018076:DNA, Complementary; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta