The determination of biogenic amines in four strains of the fruit fly Drosophila melanogaster.
A range of biogenic amines were measured in the heads from four strains of Drosophila melanogaster. Quantitation was carried out using gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) with stable isotope dilution. The principal amines detected in the heads were dopamine, noradrenaline and 5 HT with small amounts of p- and m-tyramine; p-octopamine could not be detected in samples of 25 heads with a limit of detection of 10 pg per sample. In addition to conventional neurotransmitters or putative neurotransmitters the amines 5- and 6-hydroxydopamine were detected in the heads in substantial amounts.
Watson
D G
DG
Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
Zhou
P
P
Midgley
J M
JM
Milligan
C D
CD
Kaiser
K
K
D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis*; D004298:Dopamine / Q000032:analysis; D004331:Drosophila melanogaster / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006257:Head; D006892:Hydroxydopamines / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis; D009655:Octopamine / Q000032:analysis; D012701:Serotonin / Q000032:analysis; D014439:Tyramine / Q000032:analysis
D015306:Biogenic Monoamines; D006892:Hydroxydopamines; D009655:Octopamine; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine; D014439:Tyramine