Production of fertile transgenic maize by electroporation of suspension culture cells.
Fertile, transgenic maize plants were generated by electroporation of suspension culture cells that were treated with a pectin-degrading enzyme. Electroporation of cells from two different suspension cultures, one derived from A188 X B73 and one derived from a B73-related inbred, with a plasmid containing the bar gene, resulted in high-frequency recovery of stably transformed callus lines. Plants were regenerated from thirteen transformed callus lines and transmission of bar to progeny was demonstrated.
Laursen
C M
CM
Discovery Research, DEKALB Plant Genetics, Mystic, CT 06355-1958.
Krzyzek
R A
RA
Flick
C E
CE
Anderson
P C
PC
Spencer
T M
TM
D015139:Blotting, Southern; D002478:Cells, Cultured; D018274:Electroporation; D005298:Fertility; D017343:Genes, Plant; D030821:Plants, Genetically Modified; D012098:Reproduction; D014170:Transformation, Genetic; D003313:Zea mays / Q000166:cytology / Q000235:genetics*