The myeloid-lineage specific enhancer of the mouse myeloperoxidase gene consists of three cis-elements defined as in vitro DNase I footprints.
The myeloid-lineage specific enhancer at 3.4-3.1 kb upstream of the mouse myeloperoxidase gene [1] has been further characterised. In vitro DNase I footprinting experiments revealed three protected sequences (FT-I, -II and -III) in the enhancer, associated with the proteins that are enriched in WEHI 3BD+ cells, at which the MPO gene is highly expressed; but not in two non-MPO expressing lymphocytic cell lines. Site-specific mutations at each element severely reduced the level of the reporter gene activity in a non-additive manner. This is parallel with either abolishment or alteration of the corresponding wild-type protein-DNA interaction in vitro. Consideration of the sequence motifs present in the enhancer suggests that the cis-elements defined as the in vitro DNase I footprints are likely to be novel.
Zhu
J D
JD
Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D006098:Granulocytes / Q000201:enzymology*; D008214:Lymphocytes / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009195:Peroxidase / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004247:DNA; D009195:Peroxidase; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I