title: Leucine synthesis in spinach chloroplasts: partial characterization of 2-isopropylmalate synthase.
abstract: Leucine synthesis was found to be localized in chloroplasts of higher plants. The introductory enzyme of the isopropylmalate pathway, the 2-isopropylmalate synthase, was strongly feedback-regulated by leucine. The pH optimum of the enzyme was between 7.0 and 9.0. The Km values were 5 microM for acetyl-CoA and 75 microM for 2-oxoisovalerate.
authors.affiliation: Botanisches Institut, Tierärztlichen Hochschule Hannover, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Hagelstein
authors.forename: G
authors.initials: G
authors.lastname: Schultz
mesh_terms: D015079:2-Isopropylmalate Synthase / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007930:Leucine / Q000096:biosynthesis*; D010944:Plants / Q000201:enzymology / Q000378:metabolism*
chemical_list: D015079:2-Isopropylmalate Synthase; D007930:Leucine