title: Genetic investigations in immigration cases and frequencies of DNA fragments of the VNTR systems D2S44, D5S43, D7S21, D7S22, and D12S11 in Turks.
abstract: We have included investigations of the DNA polymorphism of variable numbers of tandem repeat (VNTR) regions with restriction fragment length polymorphism (RFLP) in the genetic evaluations in immigrant cases. HinfI-digested DNA was separated by electrophoresis in agarose gels and hybridized with radiolabelled probes detecting the VNTR systems D2S44 (YNH24), D5S43 (MS8), D7S21 (MS31), D7S22 (g3), and D12S11 (MS43a). We used the matching criterion for paternity testing for the parent/child comparisons, i.e. non-match if the intra gel difference exceeded 1.25 mm. A total of 43 immigration cases involving mainly Turks were investigated with DNA technique in parallel with investigations of 10-15 conventional systems. One man was excluded from paternity by both conventional and DNA investigations. Non-exclusion was observed with both conventional and DNA systems in 97 putative mother/child pairs and in 96 putative father/child pairs. In a putative father/child combination with non-exclusion in 18 genetic systems, a single genetic inconsistency ('exclusion') in D7S21 (MS31) was observed. The frequency distributions of HinfI digested DNA fragments of the five VNTR systems in 105 Turks are presented.
authors.affiliation: Institute of Forensic Genetics, University of Copenhagen, Denmark.
authors.forename: H E
authors.initials: HE
authors.lastname: Hansen
authors.forename: N
authors.initials: N
authors.lastname: Morling
mesh_terms: D000328:Adult; D001345:Autoradiography; D002648:Child; D004247:DNA / Q000032:analysis*; D003718:Denmark; D004641:Emigration and Immigration; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D010154:Pakistan / Q000208:ethnology; D010334:Paternity; D012150:Polymorphism, Restriction Fragment Length; D014421:Turkey / Q000208:ethnology
chemical_list: D004247:DNA