One-ended insertion of IS911.
An apparently nonreplicative integration reaction mediated by the insertion sequence IS911 has been analyzed. It is shown to involve the right-end inverted repeat (IRR) of the element and sequences in the flanking vector DNA. The flanking sequences appear to behave as a surrogate IS911 end, since integration is greatly reduced when limited similarities with IRR are eliminated by site-directed mutagenesis. Data are presented which suggest that the activity of the IRR junction results from the proximity of the transposase gene and may therefore reflect preferential transposase recognition of IRR in cis.
Polard
P
P
UPR 9007 du Centre National de la Recherche Scientifique, Toulouse, France.
Seroude
L
L
Fayet
O
O
Prère
M F
MF
Chandler
M
M
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004251:DNA Transposable Elements / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012093:Replicon / Q000235:genetics; D012762:Shigella dysenteriae / Q000235:genetics; D013329:Structure-Activity Relationship
D004251:DNA Transposable Elements