Expression of HOX C homeobox genes in lymphoid cells.
The class I homeobox genes located in four clusters in mammalian genomes (HOX A, HOX B, HOX C, and HOX D) appear to play a major role in fetal development. Previous surveys of homeobox gene expression in human leukemic cell lines have shown that certain HOX A genes are expressed only in myeloid cell lines, whereas HOX B gene expression is largely restricted to cells with erythroid potential. We now report a survey of the expression patterns of 9 homeobox genes from the HOX C locus in a panel of 24 human and 7 murine leukemic cell lines. The most striking observation is the lymphoid-specific pattern of expression of HOX C4, located at the 3' end of the locus. A major transcript of 1.9 kilobases is observed in both T-cell and B-cell lines. HOX C4 expression is also detected in normal human marrow and peripheral blood lymphocytes, but not in mature granulocytes or monocytes. HOX C8 is also expressed in human lymphoid cells but is expressed in other blood cell types as well. However, the HOX C8 transcript pattern is lineage specific. These data, in conjunction with earlier findings, suggest that homeobox gene expression influences lineage determination during hematopoiesis.
Lawrence
H J
HJ
Veterans Affairs Medical Center, San Francisco, California 94121.
Stage
K M
KM
Mathews
C H
CH
Detmer
K
K
Scibienski
R
R
MacKenzie
M
M
Migliaccio
E
E
Boncinelli
E
E
Largman
C
C
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002874:Chromosome Mapping; D015870:Gene Expression; D005801:Genes, Homeobox; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000235:genetics; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger