Different roles of prostaglandins in thromboembolic processes in arterioles and venules in vivo.
The involvement of prostaglandins in thromboembolic processes, as induced by wall puncture, was studied in rabbit mesenteric arterioles and venules using intravital videomicroscopy. Inhibition of prostaglandin formation with aspirin (100 mg/kg, i.v.) significantly increased in arterioles duration of embolization (from 91 to 200 s) and number of emboli produced (from 4 to 8.5 per vessel), while rate of embolus production was not influenced. In venules, aspirin only influenced embolization rate (a significant decrease from one embolus/14 s to one/23 s). Specific blockade of TXA2-receptors by sulotroban (30 mg/kg, i.v.) only influenced the arteriolar reaction: it significantly decreased embolization duration (from 560 to 218 s) and number of emboli produced (from 23 to 10 emboli per vessel), without affecting embolization rate. These findings indicate that both platelet activating and inhibiting prostaglandins play a more important role in thromboembolism in arterioles than in venules; this suggests a difference in prostaglandin synthetic capacity between arteriolar and venular endothelium.
oude Egbrink
M G
MG
Department of Physiology, University of Limburg, Maastricht, The Netherlands.
Tangelder
G J
GJ
Slaaf
D W
DW
Reneman
R S
RS
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D001160:Arterioles / Q000293:injuries / Q000503:physiopathology*; D001241:Aspirin / Q000494:pharmacology; D011464:Epoprostenol / Q000096:biosynthesis / Q000502:physiology; D005260:Female; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007962:Leukocytes / Q000502:physiology; D008297:Male; D008643:Mesentery / Q000098:blood supply; D015539:Platelet Activation / Q000187:drug effects; D011453:Prostaglandins / Q000502:physiology*; D011817:Rabbits; D017482:Receptors, Thromboxane / Q000037:antagonists & inhibitors; D012039:Regional Blood Flow; D013449:Sulfonamides / Q000494:pharmacology; D013923:Thromboembolism / Q000503:physiopathology*; D013928:Thromboxane A2 / Q000096:biosynthesis / Q000502:physiology; D014699:Venules / Q000293:injuries / Q000503:physiopathology*; D014743:Videotape Recording
D006893:Hydroxyeicosatetraenoic Acids; D011453:Prostaglandins; D017482:Receptors, Thromboxane; D013449:Sulfonamides; D013928:Thromboxane A2; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C040624:sulotroban; D011464:Epoprostenol; D001241:Aspirin