Stabilization of the antigenicity of coxsackie B procapsids by disoxaril.
Disoxaril added to cell cultures infected with coxsackie B virus serotypes 3 or 4 increased the yield and stabilized the antigenicity of the procapsids. In the absence of disoxaril, the procapsids lost their broad antigenicity after a short period of time. Prevention of this loss by disoxaril was observed in radioimmunoassays of IgM in sera from patients infected with various enteroviruses. The procapsids are useful in serological diagnosis of enterovirus infections, and the addition of disoxaril facilitates this technique.
Frisk
G
G
Department of Medical Virology, University of Uppsala, Sweden.
Halloran
M
M
Didarholm
H
H
D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000187:drug effects*; D002213:Capsid / Q000187:drug effects / Q000276:immunology; D004355:Drug Stability; D004770:Enterovirus / Q000276:immunology; D029822:Enterovirus B, Human / Q000187:drug effects* / Q000276:immunology; D004769:Enterovirus Infections / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007555:Isoxazoles / Q000494:pharmacology*; D011533:Proviruses / Q000187:drug effects / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D014773:Virology / Q000379:methods*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007075:Immunoglobulin M; D007555:Isoxazoles; C045978:disoxaril