title: Characterization of [35S]lanthionine ketimine specific binding to bovine brain membranes.
abstract: [35S]Lanthionine ketimine binds specifically and with high affinity to bovine brain membranes. This binding has been studied in detail. It is reversible, not occurring at an uptake site or at a metabolizing enzyme and depending only weakly from ionic strength; it is affected by thiol reagents. [35S]Lanthionine ketimine specific binding is displaced only by other ketimines and by catecholamines, but not by more selective adrenergic ligands; binding parameters are reported. [3H]Adrenaline but not [3H]dihydroalprenolol is partially displaced by lanthionine ketimine. With bovine brain preparations a significant stimulation of basal adenylate cyclase activity by lanthionine ketimine is observed.
authors.affiliation: Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Università La Sapienza, Roma, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Duprè
authors.forename: M
authors.initials: M
authors.lastname: Fontana
authors.forename: M
authors.initials: M
authors.lastname: Costa
authors.forename: L
authors.initials: L
authors.lastname: Pecci
authors.forename: G
authors.initials: G
authors.lastname: Ricci
authors.forename: D
authors.initials: D
authors.lastname: Cavallini
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000603:Amino Acids, Sulfur / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism; D007700:Kinetics; D018377:Neurotransmitter Agents / Q000494:pharmacology; D013462:Sulfur Radioisotopes; D014422:Turkeys
chemical_list: D000603:Amino Acids, Sulfur; D018377:Neurotransmitter Agents; D013462:Sulfur Radioisotopes; C035499:lanthionine ketimine; D000262:Adenylyl Cyclases