title: Macrophage chemiluminescence induced by interaction with transparent and opaque colonial variants of Mycobacterium intracellulare.
abstract: Macrophage (M phi) chemiluminescence (CL) induced by interaction with the two types of colonial variants of Mycobacterium intracellulare was studied. A smooth, opaque and dome-shaped (SmD) colonial variant triggered more intense M phi CL than did a smooth, transparent and flat colonial variant (SmT). M phi CL-inducing activity of the SmD variant was reduced by heating or by treatments with either Pronase P, some endoglycosidases or Tween 80, thereby indicating that the SmD variant possesses M phi CL-inducing substance(s) having peptide, sugar and/or lipid-like moieties. Treatment of the SmD variant organism with some endoglycosidases, such as cellulase, pectinase, dextranase or alpha-amylase decreased its M phi CL-inducing ability. On the other hand, M phi CL-inducing activity of the SmT variant was not affected by any of above treatments except that it was slightly increased by Pronase P treatment and reduced by alpha-amylase and dextranase.
authors.affiliation: Department of Microbiology and Immunology, Shimane Medical University, Izumo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tomioka
authors.forename: H
authors.initials: H
authors.lastname: Saito
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D014644:Genetic Variation; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009162:Mycobacterium avium / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D010455:Peptides / Q000378:metabolism; D010641:Phenotype; D016897:Respiratory Burst; D014774:Virulence / Q000235:genetics / Q000502:physiology
chemical_list: D010455:Peptides