The 5' untranslated region of the human gamma-glutamyl transferase mRNA contains a tissue-specific active translational enhancer.
We report the functional and structural analysis of the 5' untranslated region (5'UTR) of human hepatoma HepG2 gamma-glutamyltransferase (GGT) mRNA. Transient expression of a hybrid GGT-luciferase gene in HepG2, MIA-Pa-Ca-2 and MG 63 cell lines shows that this 5'UTR acts as a tissue-specific translational enhancer. Evidence for transcripts with multiple 5'UTR coding for HepG2 GGT was obtained by RNase protection. Computer analysis of this 5'UTR detected the existence of a stable stem and loop structure containing multiple steroid modulatory elements.
Diederich
M
M
Centre du Médicament, URA CNRS 597, Nancy, France.
Wellman
M
M
Visvikis
A
A
Puga
A
A
Siest
G
G
D001483:Base Sequence; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000235:genetics; D005723:gamma-Glutamyltransferase / Q000235:genetics*
D012333:RNA, Messenger; D008156:Luciferases; D005723:gamma-Glutamyltransferase; D001616:beta-Galactosidase