Guanine nucleotide exchange factor for eukaryotic initiation factor-2. Cloning of cDNA for the delta-subunit of rabbit translation initiation factor-2B.
Peptide sequence data for rabbit eIF-2B delta were obtained and used to design redundant oligonucleotides for PCR. RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. A rabbit eIF-2B delta transcript was then amplified by PCR and sequenced. The PCR product was used to isolate a clone from a rabbit liver cDNA library. RACE (rapid amplification of cDNA ends) was used to obtain further 5' sequence. Subsequently, a full length cDNA was obtained from a rabbit reticulocyte library. PCR was used to confirm that the sequence is the same for the liver factor. The sequence obtained shows strong homology to that of yeast eIF-2B delta, the GCD2 gene product.
Price
N T
NT
Department of Biochemistry, University of Bristol, UK.
Francia
G
G
Hall
L
L
Proud
C G
CG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D015852:Eukaryotic Initiation Factor-2 / Q000737:chemistry / Q000235:genetics*; D020717:Eukaryotic Initiation Factor-2B; D005656:Fungal Proteins / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D012097:Repressor Proteins / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins
D018076:DNA, Complementary; D015852:Eukaryotic Initiation Factor-2; D020717:Eukaryotic Initiation Factor-2B; D005656:Fungal Proteins; C068999:GCD2 protein, S cerevisiae; D020662:Guanine Nucleotide Exchange Factors; D009841:Oligonucleotides; D011506:Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins