The XbaI-BlnI-CeuI genomic cleavage map of Salmonella paratyphi B.
The genomic cleavage map of Salmonella paratyphi B was determined through digestion with endonucleases and separation of the fragments by pulsed-field gel electrophoresis. The chromosome has 19 XbaI sites, 10 BlnI sites, and 7 CeuI sites. The fragments were arranged in order through excision of fragments from the gel, redigestion with a second enzyme, end labelling with 32P, and reelectrophoresis. Tn10 transposons inserted in 61 different genes of S. typhimurium LT2 were transduced by use of bacteriophage P22 into S. paratyphi B. The locations of Tn10 insertions on the chromosome of S. paratyphi B were determined by use of XbaI and BlnI sites in Tn10, revealing the positions of genes with Tn10 insertions in S. paratyphi B. All seven CeuI sites (in rrl genes for 23S rRNA) and most of the XbaI and BlnI sites in rrn genes for Glt-tRNA are conserved, but only about half of the XbaI and BlnI sites outside rrn genes are conserved. Gene order is identical in the 68 genes that we could compare between S. paratyphi B and S. typhimurium LT2, and the lengths of intervals between the genes are often the same, but there are several instances of differences in interval lengths, indicating that insertions or deletions of DNA have occurred during the evolutionary divergence of these bacteria.
Liu
S L
SL
Salmonella Genetic Stock Centre, Department of Biological Sciences, University of Calgary, Alberta, Canada.
Hessel
A
A
Cheng
H Y
HY
Sanderson
K E
KE
D017100:Bacteriophage P22 / Q000235:genetics; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D004706:Endodeoxyribonucleases / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D015183:Restriction Mapping; D012484:Salmonella paratyphi B / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004706:Endodeoxyribonucleases; C071132:endodeoxyribonuclease I-CeuI; C025580:endodeoxyribonuclease XBAI; C064053:CCTAGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific