title: VP4 genotyping of human rotavirus in the United States.
abstract: The VP4 (P) and VP7 (G) types of 171 rotavirus isolates obtained from children with diarrhea in the United States were characterized by PCR typing assays. Strains P1G1 predominated (71%); this was followed by strains P1G3 (20%) and P2G2 and P1G4 (2% each). Mixed types were identified in five (3%) specimens. Two (1%) strains bearing the P3 genotype (P3G1 and P3G2) were found in children with severe dehydrating diarrhea, although the P3 genotype has been regarded as a possible marker for virus attenuation.
authors.affiliation: Division of Molecular Biological Research and Evaluation, Food and Drug Administration, Washington, DC 20204.
authors.forename: N
authors.initials: N
authors.lastname: Santos
authors.forename: M
authors.initials: M
authors.lastname: Riepenhoff-Talty
authors.forename: H F
authors.initials: HF
authors.lastname: Clark
authors.forename: P
authors.initials: P
authors.lastname: Offit
authors.forename: V
authors.initials: V
authors.lastname: Gouvea
mesh_terms: D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002648:Child; D003967:Diarrhea / Q000235:genetics / Q000382:microbiology*; D005814:Genes, Viral / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012400:Rotavirus Infections / Q000145:classification* / Q000453:epidemiology / Q000235:genetics; D014481:United States / Q000453:epidemiology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D005819:Genetic Markers; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus