NeP1. A ubiquitous transcription factor synergizes with v-ERBA in transcriptional silencing.
One of the chicken lysozyme gene silencers binds two transcription factors, v-ERBA or the thyroid hormone receptor and NeP1 (negative protein 1), a new silencer binding protein. NeP1 is neutral on a monomeric binding site, but mediates weak repression on a multimerized site and strong synergistic repression in conjunction with v-ERBA on the wild-type silencer. Depending on the presence or absence of ligand, synergistic induction or repression is seen with the thyroid hormone receptor. This synergism is not based on cooperative DNA-binding as measured in vitro. The NeP1 DNA-binding activity is dependent on zinc ions, the binding site is characterized by a footprint of approximately 50 bp. NeP1 has a molecular weight of 140 to 160 kDa and has been enriched by affinity columns.
Köhne
A C
AC
Genetisches Institut, Giessen, Germany.
Baniahmad
A
A
Renkawitz
R
R
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D004268:DNA-Binding Proteins / Q000502:physiology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics*; D009687:Nuclear Proteins / Q000502:physiology*; D018774:Oncogene Proteins v-erbA; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology*; D012097:Repressor Proteins / Q000378:metabolism*; D015682:Retroviridae Proteins, Oncogenic / Q000502:physiology*; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000502:physiology
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D018774:Oncogene Proteins v-erbA; D012097:Repressor Proteins; D015682:Retroviridae Proteins, Oncogenic; D009113:Muramidase; D015032:Zinc