Pulmonary inflammation and immune responses during the course of Nippostrongylus brasiliensis infection: lymphocyte subsets in bronchoalveolar lavage fluids of rats.
Quantitative measurements were made of different phenotypes of lymphocytes in bronchoalveolar lavage fluid (BALF) of rats during the course of a primary or secondary infection with Nippostrongylus brasiliensis. These changes were compared with those in the peripheral blood to understand the site-specificity of the responses. Following infection, there was a significant increase in both B and T lymphocytes in BALF. The CD4:CD8 ratio was significantly altered with a decreased ratio on day 2 and increased ratio on days 16 and 32 post infection (p.i.). Two colour analysis showed that during larval migration through the lungs (day 2 p.i.) there was a significant increase in CD8+, CD4+ OX22+ and CD4+ OX22- cells in BALF. As infection progressed in time, CD4+ OX22- cells were increased significantly. Compared to primary infection, a secondary infection resulted in increased recovery of CD4+ OX22- cells in BALF. These changes were not readily appreciated in the peripheral blood, suggesting site-specific compartmentalization of lymphocyte responses in the lung. The functional significance of these dynamic changes in lymphocyte subsets in the airspaces following infection remains to be identified.
Ramaswamy
K
K
Department of Microbiology & Infectious Diseases, University of Calgary, Canada.
Befus
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D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology*; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D005434:Flow Cytometry; D008174:Lung Diseases, Parasitic / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D009559:Nippostrongylus / Q000276:immunology*; D011014:Pneumonia / Q000276:immunology* / Q000469:parasitology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017206:Strongylida Infections / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000909:Antibodies, Helminth