Anti-IL-10 antibody enhances the proliferation of CD8+ T cell clones: autoregulatory role of murine IL-10 in CD8+ T cells.
All CD8+ T cell clones used in this study secreted IL-10, and their proliferation was inhibited by exogenous IL-10. This study addresses the question of whether IL-10 produced by responding T cell clones would inhibit proliferation of the secreting T cells themselves. Anti-IL-10 antibodies enhanced the proliferative response of the CD8+ T cell clones, the enhancing effect appearing in the late period of proliferation. However, the proliferation of both CD4+ Th1 and Th2 clones was not affected by anti-IL-10 throughout the culture. In addition to that of the cloned T cells, the proliferative response of a primary culture of CD8+ T cells was enhanced by the anti-IL-10 antibody. This is the first report on a lymphokine which has an autoregulatory role in inhibiting T cell proliferation.
Nishijima
K
K
Department of Agricultural Chemistry, University of Tokyo, Japan.
Hisatsune
T
T
Minai
Y
Y
Kohyama
M
M
Kaminogawa
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000941:Antigens / Q000008:administration & dosage; D016827:CD8 Antigens; D002364:Caseins / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D005260:Female; D006706:Homeostasis / Q000276:immunology; D016753:Interleukin-10 / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D016827:CD8 Antigens; D002364:Caseins; D016753:Interleukin-10