Conformation and activity of mannose- and N-acetylgalactosamine-specific lectins from Vicia villosa seeds.
The conformation of two Vicia villosa lectins specific for mannose and N-acetylgalactosamine, respectively, was studied by circular dichroism. Both showed a broad negative CD band around 220 nm and a positive one above 190 nm. CD data analysis indicated that they were rich in beta-sheet. However, they differed in conformational stability against extreme pH, at elevated temperature, and in guanidine hydrochloride and sodium dodecyl sulfate solutions. The unusual feature was that the conformation of N-acetylgalactosamine-specific lectin was virtually unaltered in 6 M guanidine hydrochloride and 7.5 mM surfactant.
Shen
Z M
ZM
Cardiovascular Research Institute, University of California, San Francisco 94143-0130.
Shi
W X
WX
Sun
C
C
Yang
J T
JT
D000116:Acetylgalactosamine / Q000378:metabolism*; D002846:Chromatography, Affinity; D002942:Circular Dichroism; D019791:Guanidine; D006146:Guanidines / Q000737:chemistry; D006384:Hemagglutination; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D008358:Mannose / Q000378:metabolism*; D037121:Plant Lectins; D011487:Protein Conformation; D011489:Protein Denaturation; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013696:Temperature
D006146:Guanidines; D037102:Lectins; D037121:Plant Lectins; C042242:Vicia lectins; D012967:Sodium Dodecyl Sulfate; D019791:Guanidine; D000116:Acetylgalactosamine; D008358:Mannose