[Toxic effect of two antitumoral agents derived from platinum, the cisplatin and the oxaliplatin on primary cultures of proximal tubular cells of the kidney in rabbits].
There is a large field of use for cisplatin and its derivatives in the treatment of human malignancies. The nephrotoxicity is their most important use-limiting factor. The aim of this work has been to study comparatively the cisplatin and a new molecule, the oxaliplatin on primary culture of proximal tubular cells of rabbit kidney. Several markers, functional, enzymatic and biochemical have been evaluated after exposure to platinum's derivatives. The results indicates that their toxic effects are exerted on DNA and proteins synthesis, and for the cisplatin on the glucose uptake. Oxalyplatin's effect on DNA synthesis seems to be more effective, but induced a more progressive cytotoxicity. The lipids peroxidation's role with abnormalities of glutathione dependent detoxication system of the cell is possible. In conclusion, oxaliplatin seems to have a pharmacological effect more powerful than cisplatin. Its low dose effect ratio seems to promise greater safety in its use.
Legallicier
B
B
Unité INSERM 295, Faculté de Médecine de l'Université de Rouen, Saint-Etienne-du-Rouvray, France.
Leclere
C
C
Monteil
C
C
Morin
J P
JP
Fillastre
J P
JP
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology* / Q000633:toxicity; D004247:DNA / Q000187:drug effects; D003864:Depression, Chemical; D005260:Female; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000187:drug effects; D009944:Organoplatinum Compounds / Q000494:pharmacology* / Q000633:toxicity; D000077150:Oxaliplatin; D014176:Protein Biosynthesis; D011817:Rabbits
D000970:Antineoplastic Agents; D009004:Monosaccharide Transport Proteins; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D004247:DNA; D002945:Cisplatin
Action toxique de deux agents antitumoraux dérivés du platine, le cisplatine et l'oxaliplatine sur des cultures primaires de cellules tubulaires proximales de rein de lapin.