Structural and functional analysis of an Opaque-2-related gene from sorghum.
The Opaque-2 (O2) gene from maize encodes a transcriptional activator of the b-ZIP class. We have isolated and characterized a gene from sorghum, related in sequence to the O2 gene from maize. A single copy of the gene is present in sorghum. Both genomic and cDNA sequences of the O2-related sorghum gene were determined. The sequence is highly homologous to maize O2 both in the promoter and in the coding region. The most closely related sequences contain the b-ZIP domain with only 11 amino acid substitutions in a total of 122 residues. In transient expression assays, the sorghum O2-related coding sequence, expressed from a CaMV 35S promoter, activates expression from the maize b-32 promoter as effectively as that obtained with the maize O2 sequence.
Pirovano
L
L
Istituto Sperimentale per la Cerealicoltura, Sezione di Bergamo, Italy.
Lanzini
S
S
Hartings
H
H
Lazzaroni
N
N
Rossi
V
V
Joshi
R
R
Thompson
R D
RD
Salamini
F
F
Motto
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D051790:G-Box Binding Factors; D017343:Genes, Plant; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D016350:Leucine Zippers / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics*; D006109:Poaceae / Q000235:genetics*; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection; D003313:Zea mays / Q000235:genetics*
D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins; D021382:Protein Sorting Signals; D014157:Transcription Factors; C067402:opaque-2 protein, Zea mays; D005966:Glucuronidase