title: Development of neoglycoproteins conjugated with natural oligosaccharides through carboxyl residues of proteins and its application to recombinant human interleukin 1.
abstract: In order to develop glycosylated cytokines, neoglycoproteins were synthesized utilizing naturally occurring oligosaccharides. High mannose type oligosaccharide-asparagine (Asn)s, containing Man8GN2-Asn, Man7GN2-Asn and Man6GN2-Asn, were obtained from quail ovalbumin and were coupled to bovine serum albumin (BSA) by carbodiimide-mediated coupling. Major reaction occurred between amino residues of oligosaccharide-Asns and carboxyl residues of BSA. Approximately one molecules of oligosaccharides-Asns were coupled to per molecule of BSA with 50% yield of glycosylation. Among the oligosaccharides, Man6GN2-Asn appeared to be conjugated predominantly. While this strategy was applied to recombinant human interleukin 1 alpha (IL-1 alpha), three molecules of oligosaccharide-Asns were introduced into per molecule of IL-1 with 10% yield of glycosylation.
authors.affiliation: Nagoya City University College of Nursing, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ishihara
authors.forename: T
authors.initials: T
authors.lastname: Chiba
authors.forename: A
authors.initials: A
authors.lastname: Takeuchi
authors.forename: H
authors.initials: H
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Takei
authors.forename: T
authors.initials: T
authors.lastname: Takii
authors.forename: K
authors.initials: K
authors.lastname: Onozaki
mesh_terms: D000818:Animals; D002234:Carbodiimides; D002240:Carbohydrate Sequence; D003370:Coturnix; D006023:Glycoproteins / Q000138:chemical synthesis*; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007375:Interleukin-1 / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D002234:Carbodiimides; D006023:Glycoproteins; D007375:Interleukin-1; D009844:Oligosaccharides; D011993:Recombinant Fusion Proteins; C074139:1-ethyl-3-(3-dimethylaminoethyl)carbodiimide; D012710:Serum Albumin, Bovine