Hepatotoxic effects of ethanol and phenformin association in rats.
1. The association of ethanol and phenformin during 6 months in the Sprague-Dawley rat produces an alteration in lactate homeostasis. A basal blood lactate value of 54.13 +/- 15.43 mg/dl was found compared to 23.65 +/- 7.4 mg/dl in control rats. 2. Lactic acid levels increased to 28.8 +/- 7.42 mg/dl and 22.01 +/- 8.08 mg/dl after chronic administration of ethanol or phenformin in Sprague-Dawley rats, respectively. Nevertheless, these were not statistically significant with respect to those of control group. 3. The total hepatic collagen content after chronic administration of phenformin and ethanol was moderately elevated 7.12 +/- 1.85 mg/g of wet tissue, and statistically significant with respect to the control group, 4.77 +/- 1.17 mg/g. Collagen content values in phenformin and ethanol rats did not reveal statistically significant differences. 4. Liver showed histological degenerative changes but not any sign of fibrosis after chronic administration of ethanol and phenformin together.
Flores-Alvarado
L J
LJ
Departamento de Investigación, Fácultad de Medicina, Universidad de Guadalajara, Jalisco, México.
Gasca-Centeno
E
E
Ayub-Ayala
M
M
Bueno-Topete
M R
MR
Ortiz
G G
GG
Feria-Velasco
A
A
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000503:physiopathology*; D003094:Collagen / Q000096:biosynthesis; D000431:Ethanol / Q000633:toxicity*; D007773:Lactates / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D010629:Phenformin / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007773:Lactates; D000431:Ethanol; D003094:Collagen; D010629:Phenformin