Characterization of an unusually conserved AluI highly reiterated DNA sequence family from the honeybee, Apis mellifera.
An AluI family of highly reiterated nontranscribed sequences has been found in the genome of the honeybee Apis mellifera. This repeated sequence is shown to be present at approximately 23,000 copies per haploid genome constituting about 2% of the total genomic DNA. The nucleotide sequence of 10 monomers was determined. The consensus sequences is 176 nucleotides long and has an A + T content of 58%. There are clusters of both direct and inverted repeats. Internal subrepeating units ranging from 11 to 17 nucleotides are observed, suggesting that it could have evolved from a shorter sequence. DNA sequence data reveal that this repeat class is unusually homogeneous compared to the other class of invertebrate highly reiterated DNA sequences. The average pairwise sequence divergence between the repeats is 2.5%. In spite of this unusual homogeneity, divergence has been found in the repeated sequence hybridization ladder between four different honeybee subspecies. Therefore, the AluI highly reiterated sequences provide a new probe for fingerprinting in A. m. mellifera.
Tarès
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Laboratoire de Biologie des Invertébrés, Institut National de la Recherche Agronomique, Antibes, France.
Cornuet
J M
JM
Abad
P
P
D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000235:genetics*; D015139:Blotting, Southern; D017124:Conserved Sequence; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific