Expression of c-myc mRNA and protein in human endometrial carcinoma; simultaneous study of in situ hybridization and immunohistochemistry.
We examined the c-myc gene at both mRNA and protein levels in tumors of 10 endometrial carcinomas by simultaneous in situ hybridization and immunohistochemistry. Expression of c-myc mRNA was observed in all cases by in situ hybridization. Immunohistochemically, c-myc protein expression was also observed in all cases and immunoreactivity was recognized as nuclear staining. The site of c-myc protein expression was mostly consistent with that of c-myc mRNA expression. While there has been no clear correlation between the expression of c-myc mRNA and/or protein and prognostic parameters so far, this approach will contribute to an understanding of oncogene expression and its biological significance in human gynecological malignancies.
Sato
S
S
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.
Jiko
K
K
Ito
K
K
Ozawa
N
N
Yajima
A
A
Miyazaki
S
S
Sasano
H
H
D016889:Endometrial Neoplasms / Q000737:chemistry* / Q000235:genetics; D005260:Female; D016259:Genes, myc; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D011379:Prognosis; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012189:Retrospective Studies
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger