A rapid DNA extraction method from culture and clinical samples. Suitable for the detection of human cytomegalovirus by the polymerase chain reaction.
We propose an one-step DNA extraction method suitable for the polymerase chain reaction. This procedure utilizes Chelex 100, a chelating in exchange resin. This technique was compared with a traditional technique (proteinase K lysis, phenol-chloroform extraction and ethanol precipitation) for isolation of human cytomegalovirus DNA from clinical samples. The procedure using Chelex 100 appeared to be a simple and fast extraction method for human cytomegalovirus DNA.
Zandotti
C
C
Hospital de la Timone, Marseille, France.
De Lamballerie
X
X
Guignole-Vignoli
C
C
Bollet
C
C
De Micco
P
P
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000302:isolation & purification*; D006801:Humans; D007962:Leukocytes / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology; D014556:Urine / Q000382:microbiology
D004279:DNA, Viral