Cloning and sequence of the structural (hupSLC) and accessory (hupDHI) genes for hydrogenase biosynthesis in Thiocapsa roseopersicina.
The first molecular biology study on the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina is reported, namely, the construction of cosmid libraries and isolation of a hydrogenase gene cluster by hybridization with hydrogenase structural genes from the purple non-sulfur bacterium, Rhodobacter capsulatus. The sequenced gene cluster contains six open reading frames, the products of which show significant degrees of identity (from 40 to 78%) with hydrogenase gene products necessary for biosynthesis of the group-I of [NiFe]hydrogenases. The structural hupSLC genes encode the small and large hydrogenase subunits and a hydrophobic protein shown to accept electrons from hydrogenase in R. capsulatus. They are followed downstream by three genes, hupDHI, which are similar to hydrogenase accessory genes found in other bacteria.
Colbeau
A
A
Laboratoire de Biochimie Microbienne, DBMS (CNRS URA 1130 alliée à l'INSERM), Centre d'Etudes Nucléires, Grenoble, France.
Kovacs
K L
KL
Chabert
J
J
Vignais
P M
PM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002841:Chromatiaceae / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial; D015723:Gene Library; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D006864:Hydrogenase