Confirmation of the localization of the human recombination activating gene 1 (RAG1) to chromosome 11p13.
The human recombination activating gene 1 (RAG1) has previously been mapped to chromosomes 14q and 11p. Here we confirm the chromosome 11 assignment by two independent approaches: autoradiographic and fluorescence in situ hybridization to metaphase spreads and analysis of human-hamster somatic cell hybrid DNA by the polymerase chain reaction (PCR) and Southern blotting. Our results unequivocally localize RAG1 to 11p13.
Schwarz
K
K
Sektion Molekularbiologie, Universität Ulm, Germany.
Hameister
H
H
Gessler
M
M
Grzeschik
K H
KH
Hansen-Hagge
T E
TE
Bartram
C R
CR
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001483:Base Sequence; D015139:Blotting, Southern; D016466:CHO Cells; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D006224:Cricetinae; D017931:DNA Primers / Q000737:chemistry; D016654:Genes, RAG-1; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D017931:DNA Primers