A monoclonal antibody to calcitonin gene-related peptide abolishes capsaicin-induced gastroprotection.
Calcitonin gene-related peptide (CGRP) released from vasodilator nerves is implicated in the gastroprotective action of capsaicin. This experimental paradigm was used to prove the effectiveness of a monoclonal anti-CGRP antibody. The experiments were performed in anaesthetized rats in which intragastric capsaicin (0.5 mg/kg) reduced gastric injury due to ethanol (50%) by 72%. The protective effect of capsaicin was abolished by close arterial administration of the anti-CGRP antibody #4901 (5 mg) to the stomach. A monoclonal antibody to keyhole limpet haemocyanin was without effect. These data establish anti-CGRP antibody #4901 as a tool to neutralize endogenously released CGRP and show that CGRP is indispensable for the gastroprotective action of capsaicin.
Peskar
B M
BM
Department of Experimental Clinical Medicine, Ruhr-University of Bochum, Germany.
Wong
H C
HC
Walsh
J H
JH
Holzer
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology* / Q000502:physiology; D002211:Capsaicin / Q000494:pharmacology*; D000431:Ethanol / Q000633:toxicity; D005753:Gastric Mucosa / Q000187:drug effects*; D006433:Hemocyanins / Q000276:immunology; D007269:Injections, Intra-Arterial; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D000431:Ethanol; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin