PCR analysis of a three-allelic PvuII--RFLP at D4S127 closely linked to the Huntington disease locus.
The polymorphic probe BJ56PS18 defined the genetic locus D4S127 closely linked to the Huntington disease (HD) gene. BJ56PS18 detects a three-allelic polymorphism after PvuII digestion in Southern blots. For rapid genotyping in HD-families, two different PCR conditions were established.
Willoh
R
R
Institut für Humangenetik der Universität Göttingen, FRG.
Zühlke
C
C
Gerdes
B
B
Wiese
S
S
Thies
U
U
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D008040:Genetic Linkage; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length