title: Ultrastructure and cytochemical characteristics of leukocyte infected with Hepatozoon canis.
abstract: We have observed the ultrastructure of a canine leukocyte infected with Hepatozoon canis by transmission electron microscope. The infected leukocytes contained many small vesicles and a few typical granules in the cytoplasm. They also contained a few rough surfaced endoplasmic reticula, mitochondria and a segmented nucleus with condensed chromatin. Cytochemical analysis indicated that the infected leukocytes with H. canis contained little alkaline phosphatase, peroxidase, naphthol AS-D chloroacetate esterase or alpha naphthol acetate esterase. These results suggested that the infected leukocytes were neutrophils or monocytes which lost their typical characteristics.
authors.affiliation: Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Kanoe
authors.forename: Y
authors.initials: Y
authors.lastname: Kono
authors.forename: M
authors.initials: M
authors.lastname: Ishida
authors.forename: M
authors.initials: M
authors.lastname: Horio
authors.forename: M
authors.initials: M
authors.lastname: Shimada
authors.forename: M
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Y
authors.initials: Y
authors.lastname: Taura
authors.forename: S
authors.initials: S
authors.lastname: Nakama
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D004283:Dog Diseases; D004285:Dogs; D004950:Esterases / Q000097:blood; D016783:Eucoccidiida / Q000302:isolation & purification*; D007962:Leukocytes / Q000469:parasitology* / Q000473:pathology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009000:Monocytes / Q000201:enzymology / Q000473:pathology; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology; D011529:Protozoan Infections, Animal / Q000097:blood*
chemical_list: D004950:Esterases; D000469:Alkaline Phosphatase