Induction of coenzyme A-dependent transacylation activity in rat liver microsomes by administration of clofibrate.
The effect of administration of clofibrate on the activity of coenzyme A-dependent (CoA-dependent) transacylation of 1-acyl-glycerophosphocholine (1-acyl-GPC) was examined in rat liver microsomes. Administration of clofibrate to rats increased the activity of Co-A-dependent transacylation of 1-[14C]acyl-GPC and the activity reached a value (8.37 nmol/min per mg protein) twice that in control rats (3.95 nmol/min per mg protein) without any changes in apparent Km values for CoA (1.2 microM in control and 1.0 microM in clofibrate-treated) and 1-acyl-GPC (33.4 microM in control and 27.8 microM in clofibrate-treated). The rate of CoA-dependent transfer of [14C]arachidonic acid (20:4) from 1-acyl-2-[14C]20:4-glycerophosphoethanolamine (GPE) or 1-acyl-2-[14C]20:4-glycerophosphoinositol (GPI) to 1-acyl-GPC (synthesis of 1-acyl-2-[14C]20:4-GPC) was also increased by treatment with clofibrate (1.9-fold and 1.5-fold increases, respectively). These results suggest that a CoA-dependent transacylation system of 1-acyl-GPC was induced by treatment with clofibrate.
Yamashita
A
A
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Sato
K
K
Watanabe
M
M
Tokudome
Y
Y
Sugiura
T
T
Waku
K
K
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002994:Clofibrate / Q000494:pharmacology*; D003065:Coenzyme A / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D010743:Phospholipids; D000217:Acyltransferases; D002994:Clofibrate; D003065:Coenzyme A