title: Alleged reaction in gel-immunodiffusion of an IgM monoclonal antibody with alfalfa mosaic virus and cucumber mosaic virus is an artefact.
abstract: A previously reported spurious serological cross-reaction between alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV), which had been defined by the reaction in gel-immunodiffusion tests of a single IgM monoclonal antibody (MAb), MAb 8, was no longer detected in the presence of 0.1 M-NaCl. The non-specific reactivity of this IgM was also confirmed in Western blotting assays. When skimmed milk was used as a blocking agent and as a diluent of antibodies, MAb 8 failed to recognize AMV and CMV coat proteins. Hence, it is concluded that the alleged cross-reaction between AMV and CMV is due to non-specific binding of MAb 8 and that there is no evidence for a serological relationship between these two viruses.
authors.affiliation: Department of Crop Protection, Waite Agricultural Research Institute, University of Adelaide, Australia.
authors.forename: M R
authors.initials: MR
authors.lastname: Hajimorad
authors.forename: R G
authors.initials: RG
authors.lastname: Dietzgen
authors.forename: W S
authors.initials: WS
authors.lastname: Wahyuni
authors.forename: R I
authors.initials: RI
authors.lastname: Francki
mesh_terms: D017794:Alfalfa mosaic virus / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D017799:Cucumovirus / Q000276:immunology*; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M