title: Early steps of the P-glycoprotein expression in cell cultures studied with vital fluorochrome.
abstract: This study shows that flow cytometry analysis of the rate of fluorochrome Rh123 efflux may be used for detection of cells at initial steps of P-glycoprotein expression and of minor subpopulations of multidrug-resistant (MDR) variants in human cell lines. This method also evaluates the fraction of low-level MDR cells among peripheral blood leukocytes of patients with chronic myeloid leukemia. The alterations in Pgp function were revealed in rat hepatoma cells after short treatment with colchicine.
authors.affiliation: Institute of Carcinogenesis, Russian Academy of Medical Sciences, Moscow.
authors.forename: S V
authors.initials: SV
authors.lastname: Egudina
authors.forename: T P
authors.initials: TP
authors.lastname: Stromskaya
authors.forename: E A
authors.initials: EA
authors.lastname: Frolova
authors.forename: A A
authors.initials: AA
authors.lastname: Stavrovskaya
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D004351:Drug Resistance; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D008114:Liver Neoplasms, Experimental; D008545:Melanoma; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051381:Rats; D020112:Rhodamine 123; D012235:Rhodamines / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D008562:Membrane Glycoproteins; D012235:Rhodamines; D020112:Rhodamine 123; D003078:Colchicine