Neurogenic expression of snail is controlled by separable CNS and PNS promoter elements.
The Drosophila snail (sna) gene is first expressed in cells giving rise to mesoderm and is required for mesoderm formation. sna is subsequently expressed in the developing nervous system. sna expression during neurogenesis evolves from segmentally repeated neuroectodermal domains to a pan-neural pattern. We have identified a 2.8 kb regulatory region of the sna promoter that drives LacZ expression in a faithful neuronal pattern. Deletion analysis of this region indicates that the pan-neural element is composed of separable CNS and PNS components. This finding is unexpected since all known genes controlling early neurogenesis, including the proneural genes (i.e. da and AS-C), are expressed in both the CNS and PNS. We also show that expression of sna during neurogenesis is largely independent of the proneural genes da and AS-C. The separate control of CNS and PNS sna expression and independence of proneural gene regulation add to a growing body of evidence that current genetic models of neurogenesis are substantially incomplete.
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Department of Biology, University of California, San Diego, La Jolla 92093-0322.
Levine
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Bier
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D000818:Animals; D002490:Central Nervous System / Q000196:embryology; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D017344:Genes, Insect / Q000235:genetics*; D017403:In Situ Hybridization; D009420:Nervous System / Q000196:embryology*; D017933:Peripheral Nervous System / Q000196:embryology; D011401:Promoter Regions, Genetic / Q000235:genetics*