title: Demineralization of bone by ammonium chloride for the pyroantimonate method.
abstract: Rat bones were fixed by the pyroantimonate method and demineralized by treatment with ammonium chloride (NH4Cl). Bones from very young rats (neonatal and 2-week-old rats) that had been treated with NH4Cl were easy to cut into ultrathin sections. However, bones of 8-week-old rats needed to be demineralized for a longer time and were not very easy to cut. The extent of demineralization was measured by scintillation counting. After demineralization, precipitates of calcium pyroantimonate (Ca-pyroantimonate) were well retained and the fine structure of the tissue was well preserved. In addition, the distribution of Ca-pyroantimonate in the demineralized bone was similar to that in undecalcified specimens. This demineralization method is used in combination with the pyroantimonate method.
authors.affiliation: Department of Anatomy, Toyama Medical and Pharmaceutical University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kawamata
mesh_terms: D000643:Ammonium Chloride; D000818:Animals; D000831:Animals, Newborn; D000965:Antimony; D001842:Bone and Bones / Q000737:chemistry* / Q000648:ultrastructure*; D002118:Calcium / Q000302:isolation & purification; D005260:Female; D005404:Fixatives; D006652:Histological Techniques; D008297:Male; D008903:Minerals / Q000302:isolation & purification*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005404:Fixatives; D008903:Minerals; D000643:Ammonium Chloride; C009133:pyroantimonate; D000965:Antimony; D002118:Calcium