The genetic and RFLP characterization of the left end of linkage group III in Caenorhabditis elegans.
A genetic approach was taken to identify new transposable element Tc1-dependent polymorphisms on the left end of linkage group III in the nematode Caenorhabditis elegans. The cloning of the genomic DNA surrounding the Tc1 allowed the selection of overlapping clones (from the collection being used to assemble the physical map of the C. elegans genome). A contig of approximately 600-800 kbp in the region has been identified, the genetic map of the region has been refined, and 10 new RFLPs as well as at least four previously characterized genetic loci have been positioned onto the physical map, to the resolution of a few cosmids. This analysis demonstrated the ability to combine physical and genetic mapping for the rapid analysis of large genomic regions (0.5-1 Mbp) in genetically amenable eukaryotes.
Pilgrim
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MRC Laboratory of Molecular Biology, Cambridge, U.K.
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic
D005819:Genetic Markers; D004247:DNA