title: On the limits of near-equilibrium DNA gel electrophoretic sequencing.
abstract: High field intensities deeply affect the migration mechanisms of DNA in gels. The corresponding large electric forces lead to far-from-equilibrium transport properties such as the limiting mobility, increased diffusion and strong pulsed field responses. We present a theoretical study of the conditions required to retain a near-equilibrium reptation transport behaviour. We then examine the possibility of increasing both the field intensity and the gel concentration to sequence large single-stranded DNA molecules rapidly and efficiently. Finally, we discuss possible experimental approaches based on our theoretical predictions.
authors.affiliation: Department of Physics, University of Ottawa, Ontario, Canada.
authors.forename: G W
authors.initials: GW
authors.lastname: Slater
authors.forename: P
authors.initials: P
authors.lastname: Mayer
authors.forename: G
authors.initials: G
authors.lastname: Drouin
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D017136:Ion Transport
chemical_list: D004247:DNA