Effects of lipopolysaccharide on hypothalamic-pituitary-adrenal axis in vitro.
The possible involvement of lipopolysaccharide (LPS) and interleukin-1 beta (IL-1 beta) and their eventual interplay in CRH and ACTH release from cultured hypothalamic and pituitary cells respectively, have been studied. IL-1 beta was able to activate the hypothalamo-pituitary-adrenal axis at both hypothalamic and pituitary sites; LPS showed no direct action at hypothalamic level but it was able to inhibit basal and IL-1 beta-induced ACTH release: this could be responsible for a blunting of the adrenal cortex response that normally occurs in septic shock syndrome.
Brunetti
L
L
Department of Pharmacology, G. D'Annunzio University, Chieti, Italy.
Preziosi
P
P
Ragazzoni
E
E
Vacca
M
M
D000324:Adrenocorticotropic Hormone / Q000037:antagonists & inhibitors / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology / Q000502:physiology*; D008070:Lipopolysaccharides / Q000378:metabolism*; D008297:Male; D010913:Pituitary-Adrenal System / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012772:Shock, Septic / Q000503:physiopathology*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone