Aerosolized recombinant interferon-gamma prevents antigen-induced eosinophil recruitment in mouse trachea.
In a murine model of airway late-phase reaction we have previously shown that antigen-induced eosinophil recruitment into the airways of sensitized mice is mediated by CD4+ T cells and interleukin-5. To determine whether interferon-gamma (IFN-gamma) regulates antigen-induced eosinophil recruitment into the airways, we studied the effect of aerosolized recombinant murine IFN-gamma on the eosinophil infiltration of the trachea induced by antigen inhalation in mice. The administration of aerosolized rIFN-gamma prevented antigen-induced eosinophil infiltration in the trachea of sensitized mice. The administration of aerosolized rIFN-gamma also decreased antigen-induced CD4+ but not CD8+ T-cell infiltration in the trachea. However, there were no significant changes in the number of blood eosinophils or of splenic CD4+ and CD8+ T cells in the treatment with rIFN-gamma. These results indicate that locally administered IFN-gamma prevents antigen-induced eosinophil recruitment into the airways by inhibiting CD4+ T cell infiltration.
Nakajima
H
H
Second Department of Internal Medicine, Chiba University School of Medicine, Japan.
Iwamoto
I
I
Yoshida
S
S
D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D016516:CD4-CD8 Ratio / Q000187:drug effects; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology; D005260:Female; D007114:Immunization / Q000379:methods; D007371:Interferon-gamma / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D011994:Recombinant Proteins; D013997:Time Factors; D014132:Trachea / Q000187:drug effects* / Q000276:immunology
D000336:Aerosols; D000941:Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma; D010047:Ovalbumin