title: Structure and expression of the gene for the prothoracicotropic hormone of the silkmoth Bombyx mori.
abstract: We cloned and characterized two allelic variants of the gene for the Bombyx mori prothoracicotropic hormone (PTTH), a homodimeric 30-kDa brain secretory protein. These PTTH genes contain five exons that encode a precursor protein consisting of 224 amino acid residues whose C-terminal 109 residues represent the PTTH subunit. The Bombyx haploid genome contains a single copy of the PTTH gene. The major site of PTTH expression is the brain but expression at a very low level occurs in the gut. One Bombyx brain at day 0 of the fifth larval instar contained 2.4-2.8 pg PTTH mRNA, and this amount did not change markedly during larval-pupal development.
authors.affiliation: Department of Biology, School of Science, Nagoya University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Adachi-Yamada
authors.forename: M
authors.initials: M
authors.lastname: Iwami
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Ishizaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D012831:Bombyx / Q000235:genetics*; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D017931:DNA Primers; D015870:Gene Expression; D017344:Genes, Insect; D015698:Genomic Library; D007301:Insect Hormones / Q000096:biosynthesis / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D007301:Insect Hormones; D046911:Macromolecular Substances; D009479:Neuropeptides; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; C009089:prothoracicotropic hormone; D004247:DNA