Intracellular development of subperiodic Brugia malayi influenced by mosquito thoracic muscle cells.
The in vitro development of 1-day-old intracellularly lodged larvae of Brugia malayi cultured in infected excised thoraces of selected susceptible and refractory strains of Aedes aegypti and Anopheles quadrimaculatus was compared with larvae reared in vivo. In susceptible mosquitoes, both in vitro and in vivo, larvae developed normally and abnormally. In refractory mosquitoes this pattern of both normal and abnormal development was also observed, except that comparatively fewer larvae developed to the infective third-stage larvae (L3) in vitro than in vivo and that more first-stage larvae (L1) were intracellularly melanized in vivo than in vitro. These studies indicate that factors in the thoracic muscle cells of the mosquito greatly affect the development of B. malayi microfilariae to L3. Intracellular melanization of L1 in An. quadrimaculatus, previously demonstrated in vivo, rarely occurred in vitro. These studies therefore suggest that refractoriness and melanization of B. malayi larvae in the thoraces of An. quadrimaculatus are controlled by two different and separate mechanisms.
Nayar
J K
JK
Florida Medical Entomology Laboratory, IFAS, University of Florida, Vero Beach 32962.
Bradley
T J
TJ
Mikarts
L L
LL
Knight
J W
JW
D000330:Aedes / Q000469:parasitology; D000818:Animals; D000852:Anopheles / Q000469:parasitology; D017178:Brugia malayi / Q000254:growth & development*; D009033:Culicidae / Q000469:parasitology*; D007814:Larva / Q000254:growth & development; D008543:Melanins / Q000096:biosynthesis; D008842:Microfilariae / Q000254:growth & development; D009132:Muscles / Q000469:parasitology; D013909:Thorax / Q000469:parasitology
D008543:Melanins