PCM-1, A 228-kD centrosome autoantigen with a distinct cell cycle distribution.
We report the identification and primary sequence of PCM-1, a 228-kD centrosomal protein that exhibits a distinct cell cycle-dependent association with the centrosome complex. Immunofluorescence microscopy using antibodies against recombinant PCM-1 demonstrated that PCM-1 is tightly associated with the centrosome complex through G1, S, and a portion of G2. However, late in G2, as cells prepare for mitosis, PCM-1 dissociates from the centrosome and then remains dispersed throughout the cell during mitosis before re-associating with the centrosomes in the G1 phase progeny cells. These results demonstrate that the pericentriolar material is a dynamic substance whose composition can fluctuate during the cell cycle.
Balczon
R
R
Department of Structural and Cellular Biology, University of South Alabama, Mobile 36688.
Bao
L
L
Zimmer
W E
WE
D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D001483:Base Sequence; D016466:CHO Cells; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins; D006224:Cricetinae; D018076:DNA, Complementary / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D015388:Organelles / Q000502:physiology* / Q000648:ultrastructure
D001324:Autoantigens; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C086074:PCM1 protein, human