One-step conversion of amino acids into N-menthyloxycarbonyl alkyl ester derivatives for chiral gas chromatography.
Amino acids dissolved in aqueous methanol (or ethanol) and treated with optically active menthyl chloroformate were converted into N-menthyloxycarbonyl methyl (or ethyl) ester derivatives within a few minutes at room temperature. The obtained diastereomeric derivatives, with the exception of arginine and histidine, had suitable gas chromatographic properties allowing enantiomeric analysis of a large number of proteinogenic and synthetic amino acids.
Domergue
N
N
INSERM U 58, Montpellier, France.
Pugniere
M
M
Previero
A
A
D000478:Alkylation; D000596:Amino Acids / Q000737:chemistry*; D002849:Chromatography, Gas; D004952:Esters; D005561:Formates; D015394:Molecular Structure; D013237:Stereoisomerism
D000596:Amino Acids; D004952:Esters; D005561:Formates; C059663:menthyl chloroformate