Is buffering capacity the principle role of the jelly coat in Bufo arenarum fertilization?
1. Dejellied Bufo arenarum oocytes can be fertilized in Ringer-Phosphate buffer with the same efficiency as jellied (control) oocytes. 2. Ringer-Phosphate buffer at pH = 7.4 not only provides buffering capacity but also the delta pH necessary for the acrosomal reaction. 3. The use of Ringer-TRIS buffer at pH = 7.4 does not render as good as Ringer-Phosphate buffer results, in terms of fertilization percentages. 4. Insemination of oocytes in Ringer-TRIS buffer interferes with early development.
Fonovich de Schroeder
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LIBIQUIMA, Departamento de Química, Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires, Argentina.
Gauna
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Pechén de D'Angelo
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D000818:Animals; D002022:Bufo arenarum; D005260:Female; D005306:Fertilization / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000502:physiology*