Histologic appearance of the islet organoid.
The authors have attempted to develop a hybrid organoid capable of supporting the engraftment of human pancreatic islet tissue transplants. In this report, they we report the histologic appearance of this organoid structure for implantation periods up to 16 days. Both pancreatic duct-like and glandular cells were present inside the organoid. Insulin positive cells were identified within the pancreatic tissue. Although polymorphonuclear (PMN) cells were seen in association with tissue necrosis, the authors observed no lymphocyte infiltration in the area of the xenografted human pancreatic islet tissue in the implanted organoid. Endothelial cell growth factor induced neovascularization inside the organoid sufficient to support the engraftment of transplanted pancreatic islet tissue. This organoid model may provide an alternative for clinical pancreatic transplantation.
Kuo
C Y
CY
Department of Pediatrics, University of Tennessee, Memphis 38163.
Herrod
H G
HG
Burghen
G A
GA
Joyner
R
R
Myracle
A D
AD
D000818:Animals; D005260:Female; D006801:Humans; D007515:Islets of Langerhans / Q000098:blood supply / Q000473:pathology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D009940:Organoids / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014183:Transplantation, Heterologous