Synthesis and characterization of oligonucleotides containing the alpha-anomer of deoxyadenosine to study its influence on DNA replication.
The alpha-anomer of 2'-deoxyadenosine 1 is a major adenine lesion produced by hydroxyl radicals in DNA. To assess its biochemical effects on DNA replication, 1 was site-specifically incorporated into oligonucleotide templates using phosphoramidite chemistry. The templates were purified and extensively characterized to ensure their homogeneity and nucleotide sequences.
Ide
H
H
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Okagami
M
M
Murayama
H
H
Kimura
Y
Y
Makino
K
K
D001483:Base Sequence; D004261:DNA Replication / Q000187:drug effects*; D003839:Deoxyadenosines / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D013698:Templates, Genetic
D003839:Deoxyadenosines; D009841:Oligonucleotides; C058118:2'-deoxyadenosine