title: Poor prognostic value of proliferating cell nuclear antigen labelling index in breast carcinoma.
abstract: OBJECTIVE
  To investigate the association between proliferating cell nuclear antigen immunostaining and various clinicopathological variables, and its prognostic value in breast carcinoma.
  
  
  METHODS
  A monoclonal antibody PC10 was applied to formalin fixed, paraffin wax embedded tissue in 144 cases of primary breast cancer. PCNA immunostaining was scored by counting 1000 cells; the percentage of positive stained cells was recorded as the PCNA labelling index (PCNA-LI).
  
  
  RESULTS
  The PCNA-LI varied from 0-77% with a mean of 18%. When tumours were separated on the basis of the mean value, 93 had a low PCNA-LI of less or equal than 18% and 47 a high PCNA-LI of more than 18%. There was no significant correlation between PCNA-LI and all prognostic factors included in this study. Moreover, PCNA-LI failed to show any prognostic value for overall and disease free survival.
  
  
  CONCLUSIONS
  PCNA immunostaining is not correlated with clinicopathological variables and patient survival in breast cancer.
authors.affiliation: Second Department of Surgery, Kanazawa University Hospital, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Thomas
authors.forename: M
authors.initials: M
authors.lastname: Noguchi
authors.forename: H
authors.initials: H
authors.lastname: Kitagawa
authors.forename: K
authors.initials: K
authors.lastname: Kinoshita
authors.forename: I
authors.initials: I
authors.lastname: Miyazaki
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis*; D001943:Breast Neoplasms / Q000276:immunology* / Q000401:mortality; D002452:Cell Count; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen
chemical_list: D000951:Antigens, Neoplasm; D001324:Autoantigens; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen