title: Detection of Salmonella spp. in oysters by PCR.
abstract: PCR DNA amplification of a region of the himA gene of Salmonella typhimurium specifically detected Salmonella spp. In oysters, 1 to 10 cells of Salmonella spp. were rapidly detected by the PCR following a pre-enrichment step to increase sensitivity and to ensure that detection was based on the presence of viable Salmonella spp.
authors.affiliation: Department of Biology, University of Alabama at Birmingham 35294.
authors.forename: A K
authors.initials: AK
authors.lastname: Bej
authors.forename: M H
authors.initials: MH
authors.lastname: Mahbubani
authors.forename: M J
authors.initials: MJ
authors.lastname: Boyce
authors.forename: R M
authors.initials: RM
authors.lastname: Atlas
mesh_terms: D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial