A new crystal form of tropomyosin.
Tropomyosin crystals with a new morphology have been obtained from lobster tail muscle tropomyosin from which 11 residues at the carboxyl-terminus have been proteolytically removed to avoid head-to-tail polymerization. In contrast to the conventional Bailey crystal form in which the elongated tropomyosin molecules form a mesh, in the present crystals the molecules are packed side-to-side with the long axes parallel to the c-axis of the crystal. The unit cell is tetragonal with a = b = 109 A, c = 509 A, and the symmetry is either P4(1)2(1)2 or P4(3)2(1)2, with 4(1)(4(3)) helical axes parallel to the c-axis. This suggests that a group of molecules surrounding a local 4(1)(4(3)) axis is regarded as the building unit of the crystal. It is likely that the unit cell contains eight molecules with one molecule per asymmetric unit.
Miegel
A
A
European Molecular Biology Laboratory, DESY, Hamburg, Germany.
Lee
L
L
Dauter
Z
Z
Maéda
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry / Q000648:ultrastructure; D008121:Nephropidae; D017433:Protein Structure, Secondary; D011817:Rabbits; D014335:Tropomyosin / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure
D014335:Tropomyosin