[Biomicroscopy of the peritoneal tissue basophils in rats irradiated with a helium-neon laser].
Tissue basophils (T Bs) of peritoneal fluid in rats were put into a glass chamber, stained with 0.05% solution of methylene blue and examined under a microscope through out the optical axis of which a laser beam was passed (L = 632.8 nm, output power was 0.76 mW). Investigations were carried out in a regimen of continuous monitoring of single T Bs for 20 minute irradiation of cells. Dose-dependent changes in T Bs structure associated with a decrease of cellular secretory activity were observed. On minute 1, degranulation was seen in a regime of a single elimination of granules. On minute 10, mass processes of degranulation, enlightenment and decrease of metachromasia of granules due to their proteolysis were shown as well. Cells sizes were increased. If a laser beam is on a distance from 5 min to 50 min from T Bs similar changes in T Bs structure were observed but first of all they were seen on the irradiated side of a cell, and these changes were shown to be delayed proportionally to an increase of a distance between cell and laser beam.
Chertok
V M
VM
Kotsiuba
A E
AE
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D007834:Lasers; D008407:Mast Cells / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D013997:Time Factors
Biomikroskopiia peritoneal'nykh tkanevykh bazofilov krys pri obluchenii geliĭ-neonovym lazerom.