DNase I footprinting of cis- or trans-diamminedichloroplatinum(II)-modified DNA.
DNase I has been used as an enzymatic probe to visualize the conformational alteration induced in DNA by the binding of either the antitumor drug cis-platinum (cis-DDP) or the therapeutically inactive derivatives, trans-platinum (trans-DDP) and chlorodiethylene-triamineplatinum(II) (dien-Pt). We have constructed double-stranded oligonucleotides (52-mer) containing a single adduct either at the d(GG) site (cis-DDP intrastrand cross-link) or at the d(GC/GC) site (cis-DDP interstrand cross-link) or at the d(G/C) site (trans-DDP interstrand cross-link) or at the d(G) site (dien-Pt adduct). The platinated oligonucleotides are differently recognized by DNase I. As judged by DNase I, the distortions induced in the DNA double helix by the cis-DDP and trans-DDP interstrand cross-links spread over more base-pairs than that induced by the cis-DDP intrastrand cross-link.
Schwartz
A
A
Centre de Biophysique Moléculaire, C.N.R.S., Orléans, France.
Leng
M
M
D001483:Base Sequence; D002945:Cisplatin / Q000031:analogs & derivatives / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry / Q000187:drug effects*; D003850:Deoxyribonuclease I; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects; D013237:Stereoisomerism
D003432:Cross-Linking Reagents; C032799:chlorodiethylenetriamine platinum; C034697:transplatin; D004247:DNA; D003850:Deoxyribonuclease I; D002945:Cisplatin