title: Evaluation of agar gel immunodiffusion serology using caprine and ovine lentiviral antigens for detection of antibody to caprine arthritis-encephalitis virus.
abstract: The sensitivity of the agar gel immunodiffusion (AGID) test for the detection of antibody to caprine arthritis-encephalitis virus (CAEV) was investigated with CAEV or ovine progressive pneumonia virus (OPPV) as the source of antigen. A total of 218 goat serum specimens were tested for anti-CAEV antibody by AGID and immunoprecipitation of [35S]methionine-labeled CAEV. In comparison with that of immunoprecipitation, the sensitivity of the CAEV AGID test was 0.91, and that of the OPPV AGID test was 0.56. The AGID test with either antigen was 100% specific. The lower sensitivity of the OPPV AGID test in detecting caprine antibody to CAEV indicates that OPPV antigen is of limited value for use in CAEV diagnosis and control programs.
authors.affiliation: Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-7030.
authors.forename: D P
authors.initials: DP
authors.lastname: Knowles
authors.forename: J F
authors.initials: JF
authors.lastname: Evermann
authors.forename: C
authors.initials: C
authors.lastname: Shropshire
authors.forename: J
authors.initials: J
authors.lastname: VanderSchalie
authors.forename: D
authors.initials: D
authors.lastname: Bradway
authors.forename: H M
authors.initials: HM
authors.lastname: Gezon
authors.forename: W P
authors.initials: WP
authors.lastname: Cheevers
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D016088:Arthritis-Encephalitis Virus, Caprine / Q000276:immunology*; D005069:Evaluation Studies as Topic; D015511:Goat Diseases / Q000175:diagnosis / Q000276:immunology*; D006041:Goats; D005779:Immunodiffusion / Q000379:methods*; D016180:Lentivirus Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D017972:Lentiviruses, Ovine-Caprine / Q000276:immunology; D011233:Precipitin Tests; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral