Permanent increase of the GAD67/synaptophysin ratio in rat cerebral cortex nerve endings as a result of hypoxic ischemic encephalopathy sustained in early postnatal life: a confocal laser scanning microscopic study.
The aim of this study was to investigate whether perinatal hypoxia-ischemia preferentially destroys GABAergic nerve endings in rat cerebral cortex tissue which, in its turn, could then account for the reported higher risk of developing epilepsy later in life. To that end rat pups, with an age of 12-13 days postnatally, were unilaterally exposed to hypoxic-ischemic conditions. After a survival period of 2 to 6 months, the animals were sacrificed by perfusion fixation and their brains were used for cutting transversal vibratome and frozen sections. These sections were double-stained with primary antibodies against one of the two GABA synthesizing enzymes, glutamic acid decarboxylase with a mol. wt. of 66,600 (GAD67) and one of the intrinsic membrane proteins of small synaptic vesicles, synaptophysin, followed by fluorophore-conjugated second antibodies. By using the confocal laser scanning microscope, we determined the ratio between the amount of GAD67/synaptophysin immunofluorescence in nerve endings per unit volume of tissue in the hypoxia-damaged neocortex. It turned out that this ratio, contrary to expectations, was significantly higher in the hypoxia-damaged cortical areas than in matched areas on the contralateral side. It appeared, moreover, that this effect was directly proportional to the severity of the incurred damage. The conclusion was drawn that these observations do not support the hypothesis that perinatal hypoxia-ischemia ultimately leads to a preferential loss of GABAergic nerve endings in the damaged neocortex and, as such, to a shortage of inhibition.
Romijn
H J
HJ
Netherlands Institute for Brain Research, Graduate School of Neurosciences, Amsterdam ZO, The Netherlands.
van Marle
J
J
Janszen
A W
AW
D000818:Animals; D000831:Animals, Newborn; D001925:Brain Damage, Chronic / Q000209:etiology / Q000378:metabolism; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D002534:Hypoxia, Brain / Q000150:complications / Q000378:metabolism*; D007834:Lasers; D008853:Microscopy / Q000379:methods; D008970:Molecular Weight; D009411:Nerve Endings / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016708:Synaptophysin / Q000378:metabolism*; D013997:Time Factors
D016708:Synaptophysin; D005968:Glutamate Decarboxylase