Cloning and expression of interleukin-3 genes of chimpanzee and New World monkeys.
Interleukin-3 (IL-3) genes were cloned from chimpanzee (Pan troglodytes), tamarin (Saguinus oedipus) and marmoset (Callithrix jacchus) and expressed in COS cells. Although the IL-3 gene structure is well conserved in these primate species, sequence analysis revealed extensive base substitutions. The chimpanzee IL-3 protein, which is highly homologous (98.5% identity) to human IL-3, stimulated proliferation of human cells dependent on IL-3. In contrast, due to the numerous amino acid substitutions in the New World monkey IL-3 species, no stimulation of human cells was observed, illustrating the extensive evolutionary divergence of IL-3.
Burger
H
H
Department of Medical Oncology, Dr. Daniel den Hoed Cancer Center/Dijkzigt University Hospital Rotterdam, The Netherlands.
Mostert
M C
MC
Kok
E M
EM
Wagemaker
G
G
Dorssers
L C
LC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002143:Callitrichinae / Q000235:genetics; D002427:Cebidae / Q000235:genetics*; D002460:Cell Line / Q000187:drug effects; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D007377:Interleukin-3 / Q000235:genetics* / Q000494:pharmacology; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000235:genetics*; D016415:Sequence Alignment
D007377:Interleukin-3