title: Production of human matrix metalloproteinase 3 (stromelysin) in Escherichia coli.
abstract: Full-length human matrix metalloproteinase 3 (prostomelysin or proMMP-3) was produced in Escherichia coli as an intracellular insoluble aggregate that could be solubilized and refolded to yield an activatable proenzyme. The refolded protein was purified to > 95% homogeneity. The recombinant proMMP-3 (re-proMMP-3) could be activated by agents known to stimulate self-catalyzed cleavage of native fibroblast proMMP-3. The N-terminal amino-acid sequence of the re-proMMP-3 and its activation products indicated that they were the same as those obtained with the natural material.
authors.affiliation: Dupont-Merck Pharmaceutical Company, Glenolden, PA 19036.
authors.forename: S A
authors.initials: SA
authors.lastname: Rosenfeld
authors.forename: O H
authors.initials: OH
authors.lastname: Ross
authors.forename: J I
authors.initials: JI
authors.lastname: Corman
authors.forename: M A
authors.initials: MA
authors.lastname: Pratta
authors.forename: D L
authors.initials: DL
authors.lastname: Blessington
authors.forename: W S
authors.initials: WS
authors.lastname: Feeser
authors.forename: B D
authors.initials: BD
authors.lastname: Freimark
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004792:Enzyme Precursors / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism*; D005347:Fibroblasts; D006801:Humans; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
chemical_list: D018076:DNA, Complementary; D004792:Enzyme Precursors; D012333:RNA, Messenger; D011994:Recombinant Proteins; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3