[Preparation of precipitation antigens for the diagnosis of Maedi-Visna].
In the present paper antigen preparation is described to be used for diagnostics of Maedi-Visna disease by immunodiffusion test (IDT). The antigen was prepared by cultivation of the Maedi-Visna virus, OLV strain, in cell culture of the synovial membrane of carpal joint and plexus choroides of lamb. Cell cultures were used for 10-25 subcultures. The antigen was concentrated by polyethylene glycol application. The characteristics of the prepared diagnostic antigen were evaluated by its comparison with a reference, commercial preparation. Sufficient sensitivity and specificity of the prepared antigen was demonstrated by testing in a sample of 100 ovine serums.
Celer
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Vysoká skola veterinární a farmaceutická, Brno.
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis*; D002478:Cells, Cultured; D012756:Sheep; D013583:Synovial Membrane; D014776:Virus Cultivation; D014790:Visna-maedi virus / Q000276:immunology*
D000956:Antigens, Viral
Príprava precipitacního antigenu k diagnostice Maedi-Visna.