Basal expression of the human macrophage colony-stimulating factor (M-CSF) gene in K562 cells.
Macrophage colony-stimulating factor (M-CSF) functions in hematopoiesis and regulates mature monocytes. Untreated K562 leukemic cells transcribe M-CSF. Elements between bases -43 and -77 (NF-KB enhancer) and between -129 and -180 (Sp1 enhancer) of the M-CSF promoter were important for basal transcription. Gel Shift assays and ultraviolet crosslinking experiments showed a 50 kDa protein bound to the NF-KB element at -65 in a GTP-dependent fashion. Additionally, a K562 nuclear protein, specifically bound the Sp1 enhancer. Run-off transcription and half-life studies showed that M-CSF is regulated primarily at the post-transcriptional level.
Sater
R A
RA
Department of Pharmacology, Duke University, Durham, NC 27710.
D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics*; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics*; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000378:metabolism
D016328:NF-kappa B; D012333:RNA, Messenger; D016329:Sp1 Transcription Factor; D016173:Macrophage Colony-Stimulating Factor