Rotational orientation of transmembrane alpha-helices in bacteriorhodopsin. A neutron diffraction study.
The rotational orientation of the seven transmembrane alpha-helices (A-G) in bacteriorhodopsin has been investigated by neutron diffraction. The current model of bacteriorhodopsin is based on an electron density map obtained by high-resolution electron microscopy (EM). Assigning helix rotational positions in the EM model depended on fitting large side-chains, mainly aromatic residues, into bulges in the electron density map. For helix D, which contains no aromatic residues, the EM map is more difficult to interpret. For helices A and B, whose position and orientation had been determined previously by neutron diffraction, the positions defined by EM agree within experimental error with these earlier conclusions. The orientation of all seven helices has been examined by using neutron diffraction on bacteriorhodopsin samples with specifically deuterated valine, leucine and tryptophan residues. Experimental peak intensities were compared to those predicted for an extensive set of structural models. The models were generated by (1) rotating all helices around their axis; (2) moving deuterated residues in the extramembrane loops about their probable positions and changing the weight of their contribution to the neutron diffraction pattern; (3) allowing deuterated side-chains to change their conformation. The analysis confirmed exactly the positions previously determined for helices A and B. For an optimal fit to the data to be obtained, the other five helices, including helix D, must lie either at or within 20 degrees of their position in the current EM model. The complementarity of medium-resolution EM, neutron diffraction and model building for the structural study of integral membrane proteins is discussed.
Samatey
F A
FA
Institut Laue-Langevin, Grenoble, France.
Zaccaï
G
G
Engelman
D M
DM
Etchebest
C
C
Popot
J L
JL
D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000648:ultrastructure; D003461:Crystallography; D019617:Halobacterium salinarum / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008956:Models, Chemical; D009502:Neutrons; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012399:Rotation
D008565:Membrane Proteins; D001436:Bacteriorhodopsins