Genetic relationship between mouse adenovirus-2 (strain K87) and human adenovirus-2.
The DNA of mouse adenovirus strain K87 (MAd-2) was cloned and mapped with restriction endonucleases BglII, ClaI, EcoRI, HindIII and SphI. Large differences were found between the MAd-2 and MAd-1 (strain FL) DNA molecules in terms of number and location of restriction sites. The MAd-2 genome also appeared as larger in size than the MAd-1 genome (34.72 kb vs. 30.14 kb). Our results confirm the existence of two distinct adenovirus species in the mouse. Hybridization experiments, on the other hand, indicate that both MAd-1 and MAd-2 are genetically related to human adenovirus type 2 (HAd-2). Overlapping regions of DNA homology are located in genes coding for HAd-2 structural components which could explain serological relationships observed between the human and the murine adenoviruses.
Jacques
C
C
Centre de Recherche en Virologie, Institut Armand-Frappier, Laval-des-Rapides, Québec, Canada.
D'Amours
B
B
Hamelin
C
C
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D017921:Mastadenovirus / Q000235:genetics*; D051379:Mice; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D004279:DNA, Viral