Capsid destabilization is required for antibody-mediated disruption of poliovirus.
Three out of 36 poliovirus type 1-specific monoclonal antibodies which, at 37 degrees C in a medium of normal ionic strength (mu = 0.16), caused only aggregative neutralization (reversible by immune complex dissociation at pH 2) shifted to cause disruptive, acid-irreversible neutralization when the temperature was raised to 39 degrees C or the ionic strength was lowered to 1/100 of normal. Under both conditions, the antigenic conversion was stoichiometric, but the efficiency was lower at 39 degrees C than at low ionic strength. Antigenic conversion and irreversible neutralization under both conditions were inhibited by WIN 51711, a capsid-stabilizing compound. Complete inhibition required filling of most of the virion's binding pockets by this compound.
Delaet
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Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Boeyé
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A
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D002213:Capsid / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D007555:Isoxazoles / Q000494:pharmacology; D009500:Neutralization Tests; D009994:Osmolar Concentration; D017955:Poliovirus / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D013696:Temperature
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007555:Isoxazoles; C045978:disoxaril