Complete nucleotide sequence of the geminivirus tomato yellow leaf curl virus, Thailand isolate.
The complete genome of a Thailand isolate of the geminivirus tomato yellow leaf curl virus (TYLCV-Th) has been cloned and the nucleotide sequence determined. The genome consists of two DNAs each slightly greater than 2700 nucleotides in length and designated A-DNA and B-DNA. The A-DNA contains six open reading frames (ORFs) capable of encoding proteins with M(r)s greater than 10K; two ORFs were located on the B-DNA. The predicted ORFs were found at positions on the genome similar to those identified in other geminiviruses. Based upon computer-assisted sequence comparisons with other geminiviruses, TYLCV-Th maintained the greatest degree of similarity with a tomato pathogen isolated in Australia, tomato leaf curl virus. Furthermore, sequence comparisons with a large number of geminiviruses confirm further divisions of the geminiviruses based upon geographical proximity of the viruses. Agroinoculation of Nicotiana benthamiana with a TYLCV-Th A-DNA clone containing a mutation in the capsid protein ORF demonstrated that the coat protein is not essential for TYLCV-Th infection but enhances the progression of the disease.
Rochester
D E
DE
Washington University, Department of Biology, St Louis, Missouri 63130.
DePaulo
J J
JJ
Fauquet
C M
CM
Beachy
R N
RN
D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D017791:Geminiviridae / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D013785:Thailand
D004279:DNA, Viral