Buccal administration of human insulin in streptozocin-diabetic rats.
This study investigates the use of the buccal route of administration in the delivery of human insulin in rats. Streptozocin-induced diabetic female Wistar rats were used in this study. Insulin (100 U) either free (i.e., insulin solution) or associated with a carrier, namely erythrocyte-ghosts (EG) and liposomes-vesicles (LEV), was administered buccally. Blood samples were collected from the tail over a period of 5 hr. These results indicate that insulin absorption occurred, as evidenced from a decrease in blood glucose concentration, and in the case of free insulin and erythrocyte-ghosts-insulin (EG-INS). The magnitude of the blood glucose level decline was at its maximum of 39.53 mg/dl (at 2 hr) and 26.23 mg/dl (at 4 hr) for free insulin and EG-INS, respectively. No significant difference in the blood glucose level profile was observed after either LEV or liposomes-vesicles-insulin (LEV-INS). This study demonstrates the ability of human insulin to be absorbed from the mouth cavity when it is instilled in the form of a simple solution or EG-INS suspension. This absorption resulted in a definite pharmacological effect but not a significant therapeutic effect.
al-Achi
A
A
Campbell University, School of Pharmacy, Buies Creek, North Carolina 27506.
Greenwood
R
R
D000042:Absorption; D000278:Administration, Buccal; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004337:Drug Carriers; D004910:Erythrocyte Membrane; D005260:Female; D006801:Humans; D007328:Insulin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D008081:Liposomes; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D004337:Drug Carriers; D007328:Insulin; D008081:Liposomes