Molecular and antigenic characterization of HV114, a hantavirus isolated from a patient with haemorrhagic fever with renal syndrome in China.
The relationship of a Hantaan-like virus (HV114), isolated from a patient with haemorrhagic fever with renal syndrome in Hubei Province, People's Republic of China, to other pathogenic hantaviruses was evaluated by cross-neutralization studies and nucleotide sequence analysis of the M genome segment. Plaque reduction neutralization assays indicated that HV114 is closely related to prototype Hantaan (HTN) virus, strain 76-118, which was originally isolated from an Apodemus field mouse in Korea. Comparison of the M genome segments of HTN 76-118 and HV114 revealed sequence identity of 84.7% and 95.4% for nucleotides and deduced amino acids, respectively. These data demonstrate that HV114 and 76-118 are two closely related but different isolates of HTN virus, establishing the scientific basis for testing and future use in China of a recombinant vaccine expressing the genome of HTN virus strain 76-118.
Xiao
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SY
Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011.
Liang
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M
Schmaljohn
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CS
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000276:immunology; D002681:China; D006476:Orthohantavirus / Q000235:genetics* / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000382:microbiology*; D006801:Humans; D007674:Kidney Diseases / Q000382:microbiology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013577:Syndrome
D000956:Antigens, Viral