Expression of interleukin-1 alpha and beta in early passage fibroblasts from aging individuals.
Human diploid fibroblasts (HDFs) from newborn foreskin constitutively express interleukin-1 (IL-1) mRNA and protein after completing at least 70% (approximately 40 population doublings) of their in vitro life span. This IL-1 in turn induces the synthesis of specific proteins in aging HDFs. To determine whether IL-1 expression may be promoted by in vivo aging, we analyzed the expression of IL-1 and of inducible mRNAs in HDFs from two normal individuals 55 and 92 years old and in HDFs from a patient with premature aging caused by Werner's syndrome. By reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), we detected expression of IL-1 alpha and beta mRNA and protein in early passage HDFs from both normal individuals and the Werner's syndrome patient. These HDFs also expressed the IL-1-inducible mRNAs for stromelysin, plasminogen activator inhibitor type 2, manganous superoxide dismutase, and collagenase. These results suggest that an age-dependent expression of IL-1 occurs either in vivo or after a few cell divisions in vitro. Therefore, the fibroblast phenotype is modified by the expression of IL-1-inducible genes during aging.
Kumar
S
S
Department of Biological Sciences, State University of New York at Albany 12222.
Vinci
J M
JM
Millis
A J
AJ
Baglioni
C
C
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D001483:Base Sequence; D002478:Cells, Cultured; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D008875:Middle Aged; D008969:Molecular Sequence Data; D017396:Plasminogen Activator Inhibitor 2 / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D014898:Werner Syndrome / Q000378:metabolism
D007375:Interleukin-1; D017396:Plasminogen Activator Inhibitor 2; D012333:RNA, Messenger