An increase of 63 kDa-protein present in the cell membranes of Staphylococcus aureus that bears a plasmid mediating inducible resistance to partial macrolide and streptogramin B antibiotics.
A plasmid, pEP2104 (23.9 kilobase pairs), from Staphylococcus aureus carries a gene that specifies inducible resistance to 14-membered (erythromycin, EM, and oleandomycin, OL) and 16-membered macrolide (mycinamicin I and II), but not to all of the latter, and to streptogramin type B antibiotics (partial macrolide- and streptogramin-B-antibiotic resistance: PMS-resistance) (L. Jánosi, E. Bán, Acta Microbiol. Acad. Sci. Hung., 29, 187 (1982) and Y. Nakajima et al., J. Pharmacobio-Dyn., 15, 319 (1992)). The induced cells of strain 8325(pEP2104) did not inactivate EM, OL, josamycin, rokitamycin or mikamycin B (MKM-B), and the cell-free extract of the strain did not inactivate EM or MKM-B, either. Ribosomes from the cells whose PMS-resistance was induced by EM were sensitive not only to EM or spiramycin, but also to MKM-B. A 63000-dalton protein increased to a great extent only in the cell membrane fractions of induced 8325(pEP2104), and may be involved in PMS-resistance.
Matsuoka
M
M
Division of Microbiology, Hokkaido Institute of Pharmaceutical Sciences, Japan.
Jánosi
L
L
Endou
K
K
Saitoh
S
S
Hashimoto
H
H
Nakajima
Y
Y
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D018942:Macrolides; D008565:Membrane Proteins / Q000378:metabolism*; D010957:Plasmids; D012270:Ribosomes / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism*; D014769:Virginiamycin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D008565:Membrane Proteins; D014769:Virginiamycin