title: Molecular cloning and functional analysis of a cDNA coding for human DOPAchrome tautomerase/tyrosinase-related protein-2.
abstract: We have cloned the cDNAs encoding tyrosinase-related protein-2 (TRP-2) from a human melanoma cDNA library. Transient expression of the isolated cDNA in HeLa cells established that TRP-2 is DOPAchrome tautomerase. Human TRP-2/DOPAchrome tautomerase is composed of 519 amino acids with a molecular weight of 59,000 and has about 84% identity with the mouse counterpart.
authors.affiliation: Department of Applied Physiology and Molecular Biology, Tohoku University School of Medicine, Miyagi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yokoyama
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Yasumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Tomita
authors.forename: S
authors.initials: S
authors.lastname: Shibahara
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000737:chemistry; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000235:genetics*; D008545:Melanoma / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase