title: Purification and partial sequence of proteins involved in the cholic acid transport into rat liver hepatocytes.
abstract: Two proteins, in previous work labeled by affinity markers derived from taurocholic acid, were purified and partially sequenced. Antibodies were raised against purified proteins, and cross-reactions were carefully checked. The influence of these antibodies upon taurocholic acid import into vesicles from rat liver plasma membranes was measured, and showed a distinct inhibition of transport in the case of the 54 kD protein.
authors.affiliation: Biozentrum Frankfurt, Abt. Biochemie, N 210/220, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Hoffmann
authors.forename: S
authors.initials: S
authors.lastname: Müllner
authors.forename: K
authors.initials: K
authors.lastname: Ziegler
authors.forename: H
authors.initials: H
authors.lastname: Fasold
mesh_terms: D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002462:Cell Membrane; D019826:Cholic Acid; D002793:Cholic Acids / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D013656:Taurocholic Acid / Q000378:metabolism
chemical_list: D000345:Affinity Labels; D002352:Carrier Proteins; D002793:Cholic Acids; D008565:Membrane Proteins; D013656:Taurocholic Acid; D019826:Cholic Acid