Fluorometric determination of the kinetics of anthracyclines uptake by cells.
Fluorometric measurements on extracellular medium are shown to allow kinetic parameters of in vitro anthracycline uptake by cells to be calculated. The method provides influx and efflux rates, as well as the time dependence of both influx and efflux. It is applied to a normal thyroid epithelial cell line (FRTL-5) and a cell line (MPTK-6) derived from the lung metastases of a thyroid carcinoma exposed to daunorubicin at concentrations within the range of 250 to 1000 ng/ml. The results show that the number of cells influences the dependence of the kinetics upon the extracellular drug concentration and that the MPTK-6 cells are endowed with very efficient efflux mechanisms.
Andreoni
A
A
Centro Endocrinologia ed Oncologia Sperimentale-C.N.R., Napoli, Italy.
Colasanti
A
A
Kisslinger
A
A
Mastrocinque
M
M
Riccio
P
P
Roberti
G
G
D000818:Animals; D000970:Antineoplastic Agents / Q000493:pharmacokinetics; D002478:Cells, Cultured; D003470:Culture Media; D003630:Daunorubicin / Q000493:pharmacokinetics*; D004317:Doxorubicin / Q000493:pharmacokinetics*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005470:Fluorometry; D008077:Lipoproteins, LDL / Q000502:physiology; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary; D009369:Neoplasms / Q000188:drug therapy; D051381:Rats; D013050:Spectrometry, Fluorescence; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000473:pathology
D000970:Antineoplastic Agents; D003470:Culture Media; D008077:Lipoproteins, LDL; D004317:Doxorubicin; D003630:Daunorubicin