Simultaneous determination of L-693,612, a topical carbonic anhydrase inhibitor, and two potential metabolites in human whole blood by ion-pair high-performance liquid chromatography.
A method for the simultaneous determination of a topical carbonic anhydrase inhibitor, L-693,612, and two of its potential metabolites in human whole blood is described. The analytes are isolated from the matrix via liquid-liquid extraction with a mixture of toluene, ethyl acetate and isopropanol (49:50:1, v/v/v). The analytes are then back extracted into dilute phosphoric acid prior to injection into the HPLC system. A cyano column (Zorbax SB-CN, 150 x 4.6 mm) with a mobile phase of phosphoric acid(0.085%)-acetonitrile (73.5:26.5) containing 10 mM sodium decane sulfonate and adjusted to pH 3 is used for the analysis. Detection is based on UV absorbance at 252 nm. The assay was found to be linear in the concentration range of 5-500 ng/ml for each analyte when 1-ml aliquots of whole blood were extracted.
Woolf
E J
EJ
Merck Research Laboratories, Department of Drug Metabolism, West Point, PA 19486.
Au
T
T
Kasper
M
M
Constanzer
M
M
Matuszewski
B
B
D002257:Carbonic Anhydrase Inhibitors / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007477:Ions; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000097:blood*; D013876:Thiophenes / Q000097:blood*
D002257:Carbonic Anhydrase Inhibitors; D007477:Ions; D013449:Sulfonamides; D013876:Thiophenes; C086670:L 693612