title: Analysis of type II polyketide beta-ketoacyl synthase specificity in Streptomyces coelicolor A3(2) by trans complementation of actinorhodin synthase mutants.
abstract: Complementation of defined actinorhodin beta-ketoacyl synthase (KS) mutants by various other KS genes suggested that the ORF1-encoded KS may be relatively generalized in function, whereas the ORF2-encoded KS component may provide specificity in polyketide chain construction. Evidence for differential temporal-spatial expression of the actinorhodin and spore pigment KSs in Streptomyces coelicolor was obtained.
authors.affiliation: Department of Microbiology, University of Minnesota, St. Paul 55108.
authors.forename: E S
authors.initials: ES
authors.lastname: Kim
authors.forename: D A
authors.initials: DA
authors.lastname: Hopwood
authors.forename: D H
authors.initials: DH
authors.lastname: Sherman
mesh_terms: D000818:Animals; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology*; D005816:Genetic Complementation Test; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D009154:Mutation / Q000502:physiology; D016366:Open Reading Frames / Q000235:genetics / Q000502:physiology*; D051381:Rats; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D009097:Multienzyme Complexes