Plasma membrane K+/H(+)-ATPase from Leishmania donovani.
Leishmania donovani has an active K+/H+ exchange system on the surface membrane. Modulation of external K+ concentration resulted in a corresponding change in internal pH (pHi) suggesting a link between proton and potassium transport. Although a Na+/H+ antiporter is present on the plasma membrane, its sensitivity to amiloride suggests that it operates independent of K+/H+ exchange. Reduction of cellular ATP with NaN3 and KCN inhibits K+/H+ exchange showing thereby that the process is energy dependent. The K+/H+ exchange is sensitive to inhibitors of the gastric K+/H(+)-ATPase. It is concluded that the H(+)-ATPase previously reported on the plasma membrane of L. donovani is in fact a K+/H(+)-ATPase.
Jiang
S
S
Department of Biological Sciences, University of Cincinnati, Ohio 45221.
Anderson
S A
SA
Winget
G D
GD
Mukkada
A J
AJ
D000255:Adenosine Triphosphate / Q000378:metabolism; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D005452:Fluoresceins; D017506:H(+)-K(+)-Exchanging ATPase / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007893:Leishmania donovani / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D009550:Nigericin; D011188:Potassium / Q000493:pharmacokinetics / Q000494:pharmacology; D011190:Potassium Cyanide / Q000494:pharmacology; D054328:Proton Pump Inhibitors; D012977:Sodium Nitrite / Q000494:pharmacology
D005452:Fluoresceins; D054328:Proton Pump Inhibitors; D000584:Amiloride; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D000255:Adenosine Triphosphate; D017506:H(+)-K(+)-Exchanging ATPase; D012977:Sodium Nitrite; D011190:Potassium Cyanide; D009550:Nigericin; D011188:Potassium