title: Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors.
abstract: Exactly how specific splice sites are recognized during the processing of complex precursor messenger RNAs is not clear. Small nuclear ribonucleoprotein particles (snRNPs) are involved, but are not sufficient by themselves to define splice sites. Now a human protein essential for splicing in vitro, called alternative splicing factor/splicing factor 2, is shown to cooperate with the U1 snRNP particle in binding pre-mRNA. This cooperation is probably achieved by specific interactions between the arginine/serine-rich domain of the splicing factor and a similar region in a U1 snRNP-specific protein.
authors.affiliation: Department of Biological Sciences, Columbia University, New York, New York 10027.
authors.forename: J D
authors.initials: JD
authors.lastname: Kohtz
authors.forename: S F
authors.initials: SF
authors.lastname: Jamison
authors.forename: C L
authors.initials: CL
authors.lastname: Will
authors.forename: P
authors.initials: P
authors.lastname: Zuo
authors.forename: R
authors.initials: R
authors.lastname: Lührmann
authors.forename: M A
authors.initials: MA
authors.lastname: Garcia-Blanco
authors.forename: J L
authors.initials: JL
authors.lastname: Manley
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D001120:Arginine / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D012367:RNA, Viral / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D000068103:Serine-Arginine Splicing Factors
chemical_list: D009687:Nuclear Proteins; D012322:RNA Precursors; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D017412:Ribonucleoprotein, U1 Small Nuclear; D000068103:Serine-Arginine Splicing Factors; D012694:Serine; D001120:Arginine