Molecular cloning and structure of a pre-B-cell growth-stimulating factor.
Generation and proliferation of early B-cell progenitors have been known to require stromal cell-derived molecules. A stromal cell line, PA6, was found to produce a soluble mediator, which was distinct from interleukin 7 (IL-7) and stem cell factor and supported the proliferation of a stromal cell-dependent pre-B-cell clone, DW34. A cDNA clone encoding this DW34 growth-stimulating factor was isolated by expression cloning. The nucleotide sequence contained a single substantial open reading frame of 267 nucleotides encoding an 89-amino acid polypeptide. The amino acid sequence of this cytokine, designated pre-B-cell growth-stimulating factor (PBSF), revealed that it is a member of intercrine alpha subfamily. Recombinant PBSF stimulated the proliferation of DW34 cells for itself and, furthermore, synergistically augmented the growth of DW34 as well as bone marrow B-cell progenitors in the presence of IL-7.
Nagasawa
T
T
Division of Immunology, Osaka University, Japan.
Kikutani
H
H
Kishimoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001483:Base Sequence; D015152:Blotting, Northern; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D003001:Cloning, Molecular; D003470:Culture Media; D016207:Cytokines / Q000235:genetics* / Q000502:physiology; D004247:DNA; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017154:Stromal Cells / Q000166:cytology; D014162:Transfection
D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D003470:Culture Media; C515256:Cxcl12 protein, mouse; D016207:Cytokines; D004247:DNA