Development of a bispecific monoclonal antibody for use in molecular hybridisation.
A mouse hybrid hybridoma (tetradoma) was prepared by fusing hybridomas producing monoclonal antibody to acetyl-aminofluorene with hybridomas producing antibody against calf intestine alkaline phosphatase. The tetradoma line established secreted immunoglobulin manifesting parental and bispecific binding characteristics. Bispecific monoclonal antibody was purified and used for a one-step immunodetection assay of non-radioactive DNA and RNA probes. The immunoassay developed was able to detect 5 pg DNA within 2 h and gave low background noise.
Auriol
J
J
Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, Paris, France.
Guesdon
J L
JL
Mazié
J C
JC
Nato
F
F
D015073:2-Acetylaminofluorene / Q000276:immunology*; D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D018033:Antibodies, Bispecific / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D016959:Xanthomonas campestris / Q000235:genetics
D018033:Antibodies, Bispecific; D004269:DNA, Bacterial; D007074:Immunoglobulin G; D004247:DNA; D015073:2-Acetylaminofluorene; D000469:Alkaline Phosphatase