Role of glutamate dehydrogenase reaction in the control of citrate pool in yeast.
1. Role of NADP-glutamate dehydrogenase in the depletion of citrate was analyzed using permeabilized yeast cells. 2. Citrate was converted to 2-oxoglutarate, which was then metabolized to glutamate by NADP-glutamate dehydrogenase in the presence of ammonium ion. 3. Formation of 2-oxoglutarate plus glutamate was in good agreement with the concentration of citrate decreased. Glutamate formation can be a good indicator of the depletion of citrate, because 70% of the citrate decreased was converted to glutamate. 4. Glycolytic activity was closely correlated with the decrease in citrate under the in situ conditions. 5. NADP-glutamate dehydrogenase increased in anaerobically grown yeast cells. 6. An effective depletion of citrate by increased synthesis of NADP-glutamate dehydrogenase can explain the lowered mechanism of citrate causing glycolytic stimulation under the anaerobic growth conditions of yeast.
Yoshino
M
M
Department of Genetics, Institute for Developmental Research, Aichi Prefectural Colony, Japan.
Murakami
K
K
D002175:Candida / Q000201:enzymology / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000502:physiology; D002951:Citrates / Q000378:metabolism*; D019343:Citric Acid; D018086:Glutamate Dehydrogenase (NADP+) / Q000502:physiology*; D006019:Glycolysis / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*
D002951:Citrates; D019343:Citric Acid; D018086:Glutamate Dehydrogenase (NADP+)