title: Molecular cloning and sequence analysis of the gene of the molybdenum-containing aldehyde oxido-reductase of Desulfovibrio gigas. The deduced amino acid sequence shows similarity to xanthine dehydrogenase.
abstract: In this report, we describe the isolation of a 4020-bp genomic PstI fragment of Desulfovibrio gigas harboring the aldehyde oxido-reductase gene. The aldehyde oxido-reductase gene spans 2718 bp of genomic DNA and codes for a protein with 906 residues. The protein sequence shows an average 52% (+/- 1.5%) similarity to xanthine dehydrogenase from different organisms. The codon usage of the aldehyde oxidoreductase is almost identical to a calculated codon usage of the Desulfovibrio bacteria.
authors.affiliation: Instituto Gulbekian de Ciênca, Laboratório de Genética Molecular, Oeiras, Portugal.
authors.forename: U
authors.initials: U
authors.lastname: Thoenes
authors.forename: O L
authors.initials: OL
authors.lastname: Flores
authors.forename: A
authors.initials: A
authors.lastname: Neves
authors.forename: B
authors.initials: B
authors.lastname: Devreese
authors.forename: J J
authors.initials: JJ
authors.lastname: Van Beeumen
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: M J
authors.initials: MJ
authors.lastname: Romão
authors.forename: J
authors.initials: J
authors.lastname: LeGall
authors.forename: J J
authors.initials: JJ
authors.lastname: Moura
authors.forename: C
authors.initials: C
authors.lastname: Rodrigues-Pousada
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D003901:Desulfovibrio / Q000201:enzymology* / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D008982:Molybdenum; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014968:Xanthine Dehydrogenase / Q000235:genetics
chemical_list: D017931:DNA Primers; D008982:Molybdenum; D014968:Xanthine Dehydrogenase; D000445:Aldehyde Oxidoreductases