Susceptibility of human-mouse T cell hybrids to HIV-productive infection.
Interspecies human x mouse cell hybrids were used to investigate the genetic basis of human permissivity to HTLV-IIIB infection. T cell hybrids between the mouse BW 51.47 T lymphoma line and normal, PHA-IL-2 activated, human peripheral mononuclear cells (PBMCs) were generated. These hybrids preferentially segregated human chromosomes, as assessed by phenotype and karyotype analysis. Viral integration occurred only in those hybrids expressing CD4+ at the cell surface. However, infectious progeny production was demonstrated only in two of the three CD4+ hybrids tested. By segregation analysis, we could correlate the absence of human chromosomes 1, 3, and 9 with the lack of infectious viral progeny.
Ramarli
D
D
Istituto di Immunologia e Malattie Infettive, Università di Verona, Italy.
Cambiaggi
C
C
De Giuli Morghen
C
C
Tripputi
P
P
Ortolani
R
R
Bolzanelli
M
M
Tridente
G
G
Accolla
R S
RS
D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000378:metabolism; D002877:Chromosomes, Human; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015750:Genes, gag; D015658:HIV Infections / Q000235:genetics* / Q000382:microbiology; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D051379:Mice; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D016662:Virus Integration / Q000235:genetics
D015704:CD4 Antigens; D017931:DNA Primers; D004279:DNA, Viral