Effect of glucose and deoxyglucose on cytoplasmic concentration of free Ca2+ in Ehrlich ascites tumour: studies on single cells.
Concentration of free cytoplasmic Ca2+ ([Ca2+]i) in Ehrlich ascites tumour cells loaded with fura-2 was measured in single cells applying a video imaging system. In resting cells [Ca2+]i amounted to 60-340 nM and was increased after addition of 10 mM D-glucose or D-2-deoxyglucose by 80-200 nM. This increase occurred within 30-60 s following addition of the sugars and lasted for several minutes. Pretreatment of the cells with thapsigargin resulted in a much smaller [Ca2+]i increase after addition of glucose or deoxyglucose and, vice versa, thapsigargin added after the sugars mobilized less Ca2+ than when added before. A possible relation of the [Ca2+]i rise evoked by glucose and deoxyglucose to the Crabtree effect is discussed.
Czyz
A
A
M. Nencki Institute of Experimental Biology, Warsaw, Poland.
Teplova
V V
VV
Sabała
P
P
Czarny
M
M
Evtodienko
Y V
YV
Wojtczak
L
L
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000494:pharmacology; D051379:Mice; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
D013729:Terpenes; D019284:Thapsigargin; D003847:Deoxyglucose; D000252:Calcium-Transporting ATPases; D005947:Glucose; D002118:Calcium