STF-IL-4: a novel IL-4-induced signal transducing factor.
The mechanism by which interleukin-4 (IL-4) regulates the expression of particular genes is unknown. We have determined that IL-4 induces a DNA binding factor (termed STF-IL-4) which has a strong affinity for an IFN-gamma activation site (GAS). Interestingly, STF-IL-4 also binds to the IL-4 responsive promoter for the Ig heavy chain germline epsilon transcript. The IL-4 dependent activation of STF-IL-4 is rapid, does not require protein synthesis and results in the sequential appearance of binding activity first in the cytoplasm and then later in the nucleus. Activation of STF-IL-4 is sensitive to tyrosine kinase inhibitors and the active factor is tyrosine phosphorylated. This pattern of activation is similar to the activation of interferon-induced transcription factors. STF-IL-4 appears to be a new member of a growing family of cytokine-induced transcriptional regulators.
Schindler
C
C
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Kashleva
H
H
Pernis
A
A
Pine
R
R
Rothman
P
P
D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000378:metabolism; D005786:Gene Expression Regulation; D007136:Immunoglobulins / Q000378:metabolism; D015847:Interleukin-4 / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism
D004268:DNA-Binding Proteins; D007136:Immunoglobulins; D015847:Interleukin-4; D014443:Tyrosine; D004247:DNA