2-Aminopurine overrides a late telophase delay created by ectopic expression of the PITSLRE beta 1 protein kinase.
Minimal overexpression of the PITSLRE beta 1 protein kinase in CHO cells leads to a marked delay in late mitosis. We have previously shown that this delay is characterized by the presence of substantially increased numbers of tubulin midbodies, inhibition of cytokinesis, and numerous multinucleated and micronucleated cells. Others have shown that the protein kinase inhibitor 2-aminopurine (2-AP) is capable of overriding drug induced cell cycle blocks. In this study we demonstrate that the late mitotic delay and altered cellular morphology caused by ectopic expression of the PITSLRE beta 1 protein kinase can be overcome by 2-aminopurine treatment. Furthermore, 2-aminopurine inhibits PITSLRE beta 1 protein kinase activity in vivo, but does not effect p34cdc2 protein kinase activity in a similar manner.
Xiang
J
J
Department of Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, TN 38105.
Lahti
J M
JM
Kidd
V J
VJ
D015075:2-Aminopurine / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016203:CDC2 Protein Kinase / Q000378:metabolism; D016466:CHO Cells; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D006224:Cricetinae; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D008938:Mitosis / Q000187:drug effects; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010641:Phenotype; D011494:Protein Kinases / Q000096:biosynthesis* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D013692:Telophase / Q000187:drug effects; D014162:Transfection; D014404:Tubulin / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D014404:Tubulin; D015075:2-Aminopurine; D007770:L-Lactate Dehydrogenase; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D016203:CDC2 Protein Kinase