title: Murine hybridomas secreting monoclonal antibodies reacting with Mlsa antigens.
abstract: Murine monoclonal antibodies have been developed from spleens of AKR mice hyperimmunised with the monoclonal antibody against TCR-V beta 6. Two new monoclonal antibodies called AL-12-3 and AL-32-7 were able to specifically block Mlsa-induced but not MHC-induced mixed lymphocyte responses in a dose-dependent manner. Both antibodies are mouse IgG3 lambda chain isotype and bind to Mlsa-bearing lymphocytes from different strains of mice. The availability of such antibodies may well facilitate future research aimed at immunochemical and molecular characterization of Mls determinants.
authors.affiliation: Immunology Department, Rayne Institute, St Thomas' Hospital, London, UK.
authors.forename: L
authors.initials: L
authors.lastname: Di Giorgi
authors.forename: C
authors.initials: C
authors.lastname: De Matos
authors.forename: B A
authors.initials: BA
authors.lastname: Ellis
authors.forename: A
authors.initials: A
authors.lastname: Matossian-Rogers
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001667:Binding, Competitive; D006825:Hybridomas / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D016747:Minor Lymphocyte Stimulatory Antigens; D051381:Rats; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000037:antagonists & inhibitors / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D016747:Minor Lymphocyte Stimulatory Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta