Differential secretion of prostaglandin E and prostaglandin F2 alpha by ovine uninucleate trophoblast cells in vitro.
We have previously shown that ovine uninucleate trophoblast cells maintained in serum-free medium on Matrigel-coated filters in dual-compartment culture chambers retain morphological polarity similar to that found in vivo. The present study was undertaken to determine the time course of tight junction formation between these cells in vitro and to characterize their basal release of prostaglandin E and F2 alpha. Trophoblast cells were purified from ovine placentomes obtained at 117-124 days of gestation and maintained in culture for up to 10 days. On each day of culture, the permeability of the cell monolayers to 3H-inulin and the secretion of prostaglandin E and F2 alpha were determined. Minimum permeability of the cell monolayers was reached by day 4 of culture, indicating that tight junction formation was complete by this time. Following an initial decrease, prostaglandin release by the trophoblast cells stabilized on days 6 to 8 of culture. On these days, prostaglandin E was secreted in equal amounts into the apical and basal compartments of the culture chamber. There was, however, 3.5 times more prostaglandin F2 alpha secreted into the apical chamber than the basal chamber at this stage of culture. These findings confirm the functional polarity of ovine trophoblast cells in culture and demonstrate differential secretion of prostaglandin E and F2 alpha by these cells.
Ralph
M M
MM
Department of Physiology, Monash University, Clayton, Victoria, Australia.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D016764:Cell Polarity / Q000502:physiology; D015237:Dinoprost / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007365:Intercellular Junctions / Q000378:metabolism / Q000648:ultrastructure; D007444:Inulin / Q000493:pharmacokinetics; D010539:Permeability; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
D007444:Inulin; D015237:Dinoprost; D015232:Dinoprostone