Adaptation to extreme environments: structure-function relationships in Emperor penguin haemoglobin.
The functional properties of the single haemoglobin (Hb) of Emperor penguin (Aptenodytes forsteri) have been investigated at different temperatures as a function of proton and organic phosphate concentration. The complete amino acid sequence has been established. Comparison with that of human HbA shows 12 substitutions in the contact regions of alpha beta dimers. In addition to overall similarities shared with most of the avian Hbs previously described, this Hb shows significant differences, which could be related to the peculiar behaviour of this penguin. In particular we may consider that: (1) the shape of the Bohr effect curve seems well adapted for gas exchange during very prolonged dives, preserving penguin Hb from a sudden and not controlled stripping of oxygen; (2) the very minor enthalpy change observed at lower pH could be an example of molecular adaptation, through which oxygen delivery becomes essentially insensitive to exposure to the extremely low temperatures of the environment. Moreover, the small alkaline Bohr effect has been found to be only chloride-linked, since the pH dependence of the oxygen affinity is totally abolished in the absence of this ion. These functional characteristics are discussed on the basis of the primary structure of alpha and beta-chains.
Tamburrini
M
M
Institute of Protein Biochemistry and Enzymology, C.N.R., Naples, Italy.
Condò
S G
SG
di Prisco
G
G
Giardina
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds; D006454:Hemoglobins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007294:Inositol / Q000378:metabolism; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism; D010108:Oxyhemoglobins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D016415:Sequence Alignment; D017421:Sequence Analysis; D013329:Structure-Activity Relationship; D013696:Temperature; D013816:Thermodynamics
D006454:Hemoglobins; D010108:Oxyhemoglobins; D007294:Inositol; D010100:Oxygen