title: Cryopreservation of vervet monkey semen and recovery of progressively motile spermatozoa.
abstract: A method to cryopreserve semen from the Vervet monkey (Cercopithecus aethiops) has been developed, yielding a mean post thaw recovery of 63.60% of pre-freeze progressive motility. The extender contained a TES-TRIS buffer, egg yolk, dextrose, streptomycin, penicillin, and glycerol as cryoprotectant. The pH ranged from 7.10-7.18 and the osmolality was 330-345 mOsmol/L.
authors.affiliation: Experimental Biology Program, Primate Unit, MRC Tygerberg, South Africa.
authors.forename: J V
authors.initials: JV
authors.lastname: Seier
authors.forename: E
authors.initials: E
authors.lastname: Conradie
authors.forename: E E
authors.initials: EE
authors.lastname: Oettle
authors.forename: J E
authors.initials: JE
authors.lastname: Fincham
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003451:Cryoprotective Agents; D007202:Indicators and Reagents; D008297:Male; D012662:Semen Preservation / Q000295:instrumentation / Q000379:methods*; D013081:Sperm Motility; D013696:Temperature; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents; D007202:Indicators and Reagents