title: Dynamics of mitotic activity and expression of viral proteins gp51 and p24 of bovine leukemia virus producing cells.
abstract: The dynamics of expression of viral proteins gp51 and p24, virus particle production and mitotic activity of a highly productive bovine leukemia virus clone of the fetal lamb kidney (FLK) cell line were studied. The period of the highest protein production (20-44 h after incubation) was established by the indirect immunofluorescence method using specific monoclonal antibodies. It was followed by a complete formation of the cell monolayer and the most intensive period of the mitotic activity determined by the 3H-thymidine labeling method. After the active synthesis of viral protein, the formation of mature viral particles and their shedding in the intercellular spaces was established electron-microscopically and by the syncytia induction test. A similar comparative study was carried out with a BLV producing short-term lymphocyte culture (STLC).
authors.affiliation: Institute of General and Comparative Pathology, Bulgarian Academy of Sciences, Sofia.
authors.forename: R
authors.initials: R
authors.lastname: Roussev
authors.forename: M
authors.initials: M
authors.lastname: Polianova
authors.forename: D
authors.initials: D
authors.lastname: Portetelle
authors.forename: O
authors.initials: O
authors.lastname: Ivanovna
mesh_terms: D000818:Animals; D002415:Cats; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D001909:Leukemia Virus, Bovine / Q000378:metabolism / Q000648:ultrastructure*; D008938:Mitosis; D012756:Sheep; D014759:Viral Envelope Proteins / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins