Elevation of the phospholipase A2 activity in peritoneal fluid cells from women with endometriosis.
OBJECTIVE
To assess the prostaglandin (PG) production on peritoneal fluid (PF) cells, phospholipase A2 (PLA2) activity of those cells in women with endometriosis was measured and compared with that of women without endometriosis.


METHODS
Prospective clinical controlled study.


METHODS
Women who underwent laparoscopy and were found either to have endometriosis (n = 15) or not (n = 9) were included in this study. Mononuclear cells obtained from the patients at laparoscopy were immediately separated by a Ficoll-Paque technique, lysed by nitrogen cavitation, and stored at -80 degrees C.


METHODS
Phospholipase A2 activity was measured by Dole assay using 1-palmitoyl-2-[1-14C] palmitoyl phosphatidyl choline and assessed on a protein basis and a cell number basis.


RESULTS
There were at least four measurable kinds of PLA2 activity detected in the cells: two calcium-dependent pH optima 7.0 and 9.0 activities and two calcium-independent pH optima 7.5 and 8.5 activities. A calcium-dependent and pH optima 9.0 activity was the highest, and it was significantly higher in women with endometriosis when compared with those who did not have endometriosis.


CONCLUSIONS
These results indicate that the increase in the PGs in PF with endometriosis may be produced by PF cells in which PLA2 activity is elevated.
Sano
M
M
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Morishita
T
T
Nozaki
M
M
Yokoyama
M
M
Watanabe
Y
Y
Nakano
H
H
D000328:Adult; D001202:Ascitic Fluid / Q000166:cytology* / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology; D004715:Endometriosis / Q000201:enzymology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D017737:Macrophages, Peritoneal / Q000201:enzymology; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011446:Prospective Studies
D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium