title: Processing of interleukin-1 beta in synovial cells freshly isolated from patients with rheumatoid arthritis.
abstract: This study examined interleukin-1 beta (IL-1 beta) production by synovial cells from patients with rheumatoid arthritis (RA). Freshly isolated, plastic-adherent, synovial cells from ten of thirteen patients with RA secreted significant levels of IL-1 bioactivity, and the cells from five patients secreted more IL-1 beta bioactivity than IL-1 alpha bioactivity. Further, there was a relatively good correlation between IL-1 beta bioactivity and IL-1 beta converting enzyme (ICE) activity, suggesting that bioactive IL-1 beta is possibly generated through the action of ICE. Analysis of metabolically-labeled IL-1 by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), however, indicated that, although these cells contained both pre IL-1 alpha and beta intracellularly, they secreted predominantly pre IL-1 beta.
authors.affiliation: Department of Biomolecular Science, Faculty of Science, Toho University, Chiba, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Ichijo
authors.forename: H
authors.initials: H
authors.lastname: Nagatake
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Nishizawa
authors.forename: M
authors.initials: M
authors.lastname: Goto
mesh_terms: D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000378:metabolism* / Q000601:surgery; D001681:Biological Assay; D020170:Caspase 1; D002460:Cell Line; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D007719:Knee Joint / Q000601:surgery; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008666:Metalloendopeptidases / Q000378:metabolism; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D013583:Synovial Membrane / Q000276:immunology / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D020170:Caspase 1; D008666:Metalloendopeptidases