title: Monitoring of the cellular immune system in patients with biventricular assist devices awaiting cardiac transplantation.
abstract: Lack of objective parameters to predict the clinical course and outcome are a major problem in managing the patients selected for BVAD-support as a bridge to heart transplantation. This study was intended to assess whether cellular immune parameters have a predictive value for the clinical result of VAD-support. Various cellular immune markers were monitored by multiparameter cytofluorometry in 30 patients who received a VAD system (Berlin Heart). We did not find significant differences in preoperative values of immune parameters between groups of survivors (n = 14) and non-survivors (n = 16). All 9 patients who died of septic multiple organ failure (MOF) had shown increased levels of T-cell activation (CD 71, CD 25, HLA-DR) as well as leukocytosis and 7 patients who died of noninfectious complications (mostly hemorrhage or cerebral complications) had exhibited T-lymphopenia. Seven of 9 patients who died of septic MOF had extremely decreased levels of HLA-DR+ monocytes (< 30%) while all 14 survivors and all 7 patients who died of noninfectious complications showed almost normal monocytic HLA-DR antigen expression, antigen-presenting capacity and cytokine secretion. These observations point to the reduced antimicrobial immunity ("immunoparalysis") in the non-survivors and may explain the fatal course of infection in these individuals. The in vitro results of restitution experiments call for new therapeutic strategies to improve the survival of VAD-patients.
authors.affiliation: German Heart Institute, Department of Thoracic, Berlin.
authors.forename: M
authors.initials: M
authors.lastname: Hummel
authors.forename: W D
authors.initials: WD
authors.lastname: Döcke
authors.forename: N
authors.initials: N
authors.lastname: Friedel
authors.forename: R
authors.initials: R
authors.lastname: von Baehr
authors.forename: R
authors.initials: R
authors.lastname: Hetzer
authors.forename: H D
authors.initials: HD
authors.lastname: Volk
mesh_terms: D000293:Adolescent; D000328:Adult; D002423:Cause of Death; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D016027:Heart Transplantation; D006353:Heart-Assist Devices; D006801:Humans; D007111:Immunity, Cellular; D007964:Leukocytosis / Q000209:etiology; D008297:Male; D008875:Middle Aged; D015166:Monitoring, Immunologic; D009102:Multiple Organ Failure / Q000209:etiology / Q000276:immunology / Q000401:mortality
chemical_list: D006684:HLA-DR Antigens