title: Distribution of cholinergic and dopaminergic receptors in rainbow trout pineal gland.
abstract: The involvement of multiple receptors in modulating the function of the pineal gland was investigated by searching for dopaminergic and cholinergic receptors in trout pineal gland. Dopamine D1 and D2 receptors were measured using [3H]SCH23390 and [3H]spiperone, respectively. Muscarinic and nicotinic cholinergic receptors were measured using quinuclidinyl benzilate ([3H]QNB) and [3H]methylcarbamyl choline, respectively. High-affinity choline uptake sites were measured using [3H]hemicholinium-3. The distribution of dopaminergic receptors varied throughout the pineal gland in that the density of D2 receptors, which was higher than that of D1 receptors, was most abundant in the distal region, exhibiting a value of 112 +/- 17 fmol/mg tissue. The distribution of both muscarinic and nicotinic receptors was uniform throughout the pineal gland. However, the highest value for the high-affinity choline transporter (106 +/- 17 fmol/mg tissue) occurred in the proximal portion of the trout pineal gland. The results of these studies indicate that the pineal gland should not be viewed as a homogeneous tissue possessing identical density of various receptors. Furthermore, these results, along with previous data, are interpreted to suggest that different regions of pineal gland may indeed possess unique functions.
authors.affiliation: Department of Pharmacology, University of Nebraska College of Medicine, Omaha 68198-6260.
authors.forename: M
authors.initials: M
authors.lastname: Samejima
authors.forename: H K
authors.initials: HK
authors.lastname: Happe
authors.forename: L C
authors.initials: LC
authors.lastname: Murrin
authors.forename: R F
authors.initials: RF
authors.lastname: Pfeiffer
authors.forename: M
authors.initials: M
authors.lastname: Ebadi
mesh_terms: D000818:Animals; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010870:Pineal Gland / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000378:metabolism
chemical_list: D011950:Receptors, Cholinergic; D011954:Receptors, Dopamine; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic