title: Localization in the amygdala of the amnestic action of diazepam on emotional memory.
abstract: It is well known that systemically administered benzodiazepines (BZDs) induce anterograde amnesia in a variety of learning tasks. BZs effects are mediated through the GABAA complex by enhancing GABA-induced synaptic inhibition. As the GABAergic system in the amygdaloid complex (AC) is a site of action for the anxiolytic effects of BZs, such findings suggest that BZs may also influence memory through the amygdala. The present report summarizes a recent series of experiments designed to examine this implication. In a first experiment rats received either sham or bilateral AC lesion using N-methyl-D-aspartic acid (NMDA). One week later, animals were trained on an inhibitory avoidance task and tested 48 h later. Diazepam (DZP; 1.0 and 2.0 mg/kg, i.p.) or vehicle was injected 30 min prior to acquisition. The results demonstrate that DZP-induced retention deficits was blocked in rats with AC lesions. In a second experiment, in an attempt to localize the site of BZDs amnestic action in the AC, we tested the effects of DZP in rats with bilateral ibotenic acid-induced lesions of central (CE), lateral (LAT) or basolateral (BL) amygdala nuclei. The results shown that retention was impaired in animals with CE and LAT lesions but not in animals with BL lesions. In a third experiment we tested the effects of DZP microinjections in different nuclei of the AC on retention performance of rats trained in an avoidance task. The results demonstrate that DZP microinjection prior training in the BL/LAT, but not CE nuclei produce anterograde amnesia.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratory of Psychobiology, FFCLRP, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Tomaz
authors.forename: H
authors.initials: H
authors.lastname: Dickinson-Anson
authors.forename: J L
authors.initials: JL
authors.lastname: McGaugh
authors.forename: M A
authors.initials: MA
authors.lastname: Souza-Silva
authors.forename: M B
authors.initials: MB
authors.lastname: Viana
authors.forename: F G
authors.initials: FG
authors.lastname: Graeff
mesh_terms: D000647:Amnesia / Q000139:chemically induced*; D000679:Amygdala / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D003975:Diazepam / Q000494:pharmacology*; D004644:Emotions / Q000187:drug effects*; D051381:Rats
chemical_list: D003975:Diazepam