Modulation of K(+)-channels in p-neurones of the leech CNS by phosphorylation.
Two types of potassium channels of identified (p-) neurones of the leech (Hirudo medicinalis) were investigated by using the patch-clamp technique. The open-state probability of these channels in cell-attached patches can be reduced by addition of 5-hydroxytryptamine to the bath solution. After excising the patches the application of alkaline phosphatase to the cytosolic face of the patch increases the open probability. The 5-HT1A-receptor agonist buspirone mimics the effect of 5-HT. Our experiments show that the effect of 5-HT might be due to a channel phosphorylation via a 5-HT1A-receptor subtype.
Goldermann
M
M
Universität Hohenheim, Institut für Zoophysiologie (230), Stutgart, Germany.
Hanke
W
W
Schlue
W R
WR
D000469:Alkaline Phosphatase / Q000494:pharmacology; D000818:Animals; D002065:Buspirone / Q000494:pharmacology; D002490:Central Nervous System / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004594:Electrophysiology; D066298:In Vitro Techniques; D007700:Kinetics; D007865:Leeches / Q000502:physiology*; D008465:Mechanoreceptors / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology
D015221:Potassium Channels; D012701:Serotonin; D000469:Alkaline Phosphatase; D002065:Buspirone