The 'tubulin-like' S1 protein of Spirochaeta is a member of the hsp65 stress protein family.
A 65-kDa protein (called S1) from Spirochaeta bajacaliforniensis was identified as 'tubulin-like' because it cross-reacted with at least four different antisera raised against tubulin and was isolated, with a co-polymerizing 45-kDa protein, by warm-cold cycling procedures used to purify tubulin from mammalian brain. Furthermore, at least three genera of non-cultivable symbiotic spirochetes (Pillotina, Diplocalyx, and Hollandina) that contain conspicuous 24-nm cytoplasmic tubules displayed a strong fluorescence in situ when treated with polyclonal antisera raised against tubulin. Here we summarize results that lead to the conclusion that this 65-kDa protein has no homology to tubulin. S1 is an hsp65 stress protein homologue. Hsp65 is a highly immunogenic family of hsp60 proteins which includes the 65-kDa antigens of Mycobacterium tuberculosis (an active component of Freund's complete adjuvant), Borrelia, Treponema, Chlamydia, Legionella, and Salmonella. The hsp60s, also known as chaperonins, include E. coli GroEL, mitochondrial and chloroplast chaperonins, the pea aphid 'symbionin' and many other proteins involved in protein folding and the stress response.
Munson
D
D
Molecular and Cellular Biology Program, University of Massachusetts, Amherst 01003.
Obar
R
R
Tzertzinis
G
G
Margulis
L
L
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics; D015153:Blotting, Western; D018834:Chaperonin 60; D018833:Chaperonins; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007313:Insecta / Q000382:microbiology; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013142:Spirochaeta / Q000737:chemistry* / Q000235:genetics; D014404:Tubulin / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D014404:Tubulin; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins