Elevated blood pressure and craniofacial abnormalities in mice deficient in endothelin-1.
The endothelin-1 (ET-1) gene was disrupted in mouse embryonic stem cells by homologous recombination to generate mice deficient in ET-1. These ET-1-/- homozygous mice die of respiratory failure at birth and have morphological abnormalities of the pharyngeal-arch-derived craniofacial tissues and organs. ET-1+/- heterozygous mice, which produce lower levels of ET-1 than wild-type mice, develop elevated blood pressure. These results suggest that ET-1 is essential for normal mouse development and may also play a physiological role in cardiovascular homeostasis.
Kurihara
Y
Y
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Kurihara
H
H
Suzuki
H
H
Kodama
T
T
Maemura
K
K
Nagai
R
R
Oda
H
H
Kuwaki
T
T
Cao
W H
WH
Kamada
N
N
D000818:Animals; D001483:Base Sequence; D001794:Blood Pressure / Q000235:genetics / Q000502:physiology*; D001934:Branchial Region / Q000002:abnormalities* / Q000196:embryology; D017931:DNA Primers; D005314:Embryonic and Fetal Development / Q000235:genetics; D016232:Endothelins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D012131:Respiratory Insufficiency / Q000235:genetics; D012886:Skull / Q000002:abnormalities; D013234:Stem Cells
D017931:DNA Primers; D016232:Endothelins