title: Lung and systemic oxidant and antioxidant activity after graded smoke exposure in the rat.
abstract: We wanted to determine the effect of a graded smoke inhalation on lung and systemic oxidant stress, and its relationship to physiological and histological change. Male Wistar rats were given 12 breaths of 10 ml/kg (n = 8) (group 1) or 20 ml/kg (n = 8) (group 2) tidal volume, using cotton toweling smoke through the trachea using positive pressure. Rats were monitored, then killed at 24 hr. Data were compared to controls (n = 8). Peak group 1 and group 2 carboxyhemoglobins were 22 +/- 6 and 46 +/- 6%, with a mortality prior to 24 hr of 14% and 50%, respectively. Group 1 rats showed only moderate lung dysfunction but with severe airway inflammation and edema, alveolar inflammation and atelectasis, with a decrease in PaO2 from the control of 96 +/- 4 to 72 +/- 5 torr. No increase in lung, liver, or kidney oxidant-induced lipid peroxidation, measured as malondialdehyde lung, liver, or kidney oxidant-induced lipid peroxidation, measured as malondialdehyde (MDA), or decrease in the antioxidant defenses catalase was noted. Group 2 rats demonstrated severe airways edema, alveolar atelectasis, and alveolar edema, and a PaO2 decreasing below 60 torr, corresponding with a 3-fold increase in lung tissue MDA and 35% decrease in catalase. In addition, liver and kidney tissue MDA doubled, and catalase activity decreased by 40%. Increased oxygen consumption was also demonstrated.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Longwood Area Trauma Center, Brigham and Women's Hospital, Boston, Massachusetts.
authors.forename: C
authors.initials: C
authors.lastname: Lalonde
authors.forename: L
authors.initials: L
authors.lastname: Picard
authors.forename: C
authors.initials: C
authors.lastname: Campbell
authors.forename: R
authors.initials: R
authors.lastname: Demling
mesh_terms: D000336:Aerosols; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D016877:Oxidants / Q000378:metabolism*; D010101:Oxygen Consumption; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D012906:Smoke / Q000032:analysis; D015208:Smoke Inhalation Injury / Q000378:metabolism / Q000473:pathology
chemical_list: D000336:Aerosols; D000975:Antioxidants; D016877:Oxidants; D012906:Smoke; D002374:Catalase