Analysis of urinary organic acids by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.
We developed a new method for the rapid determination of urinary organic acids using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. Mass spectra of authentic organic acids obtained in the negative-ion mode showed intense [M-H]- ions with some fragment ions. Urine samples of patients with methylmalonic aciduria, ornithine transcarbamylase deficiency, and phenylketonuria were extracted using anion-exchange columns. The mass chromatograms of the extracts showed some dominant peaks of abnormal metabolites characteristic of each disorder. This is a useful method for the analysis of urinary organic acids for the diagnosis of organic aciduria, because the sample preparation is simple.
Kajita
M
M
Department of Pediatrics, Nagoya University School of Medicine, Japan.
Niwa
T
T
Watanabe
K
K
D000143:Acids / Q000652:urine*; D000293:Adolescent; D000328:Adult; D001274:Atmospheric Pressure; D001565:Benzoates; D002648:Child; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008661:Metabolism, Inborn Errors / Q000652:urine; D008764:Methylmalonic Acid / Q000652:urine; D020163:Ornithine Carbamoyltransferase Deficiency Disease; D010661:Phenylketonurias / Q000652:urine
D000143:Acids; D001565:Benzoates; D008764:Methylmalonic Acid; C022893:4-fluorobenzoic acid