Presence of the factor that inhibits collagen synthesis and stimulates cell proliferation in developing embryonic chick skin extracts.
The effects were studied of the extracts prepared from 10- and 18-day developing chick skins on collagen synthesis and proliferation of the monolayered fibroblasts established from 10-day skin. Cell proliferation was stimulated by both 10- and 18-day skin extracts at the concentration of 3.5 micrograms/ml. Collagen synthesis was suppressed by the treatment of extracts prepared from both 10- and 18-day skins at the concentration of 3.5 micrograms/ml, to a greater extent in 18-day extract. The results indicate that skin extracts possess factors exhibiting both a stimulating effect on cell proliferation and an inhibitory effect on collagen synthesis.
Tajima
S
S
Dept. of Dermatology, Keio University School of Medicine, Tokyo, Japan.
Ohyama
K
K
Tokimitsu
I
I
Takehana
M
M
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D006133:Growth Substances / Q000032:analysis / Q000494:pharmacology*; D012867:Skin / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D014020:Tissue Extracts
D006133:Growth Substances; D014020:Tissue Extracts; D003094:Collagen