Double labelling of major histocompatibility complex molecules and lysosomal protein lamp-1 on human dendritic cells.
In this study, double labelling for major histocompatibility complex (MHC) class I and class II molecules and for MHC molecules and the lysosomal membrane protein lamp-1 on ultrathin cryosections of dendritic cells isolated from human peripheral blood was performed. The plasma membrane proved to be positive for both MHC class I and MHC class II molecules and was labelled for only a very few lamp-1 molecules. MHC class I and MHC class II molecules did not co-localize intracellularly except in some peripherally located vesicles. However, many MHC class II-labelled vesicles were present in a juxtanuclear position but only some of them were co-labelled for lamp-1. These results indicate the presence of a separate, non-lysosomal compartment for class II molecules in dendritic cells.
Arkema
J M
JM
Department of Cell Biology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands.
Schadee-Eestermans
I L
IL
Broekhuis-Fluitsma
D M
DM
Fukuda
M
M
Hoefsmit
E C
EC
D015703:Antigens, CD; D003713:Dendritic Cells / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure; D005629:Frozen Sections; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology; D016253:Microscopy, Immunoelectron
D015703:Antigens, CD; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins