title: Preparation and physical properties of conjugates of oligodeoxynucleotides with poly(delta)ornithine peptides.
abstract: Oligodeoxynucleotides (12-mers) having either a 3' aminolinker or both 3' and 5' aminolinker groups were synthesized and then reacted with N-iodoacetoxysuccinimide. Separately, a series of peptides, consisting of cysteine carboxyamide and a varying number of residues of ornithine coupled through their side-chain amino groups, was prepared, leaving on the final Fmoc protecting group. Reaction of each Fmoc-peptide with an activated oligodeoxynucleotide yielded the desired conjugates, which were purified by an Fmoc-on/Fmoc-off two-step reversed-phase HPLC procedure. On hybridization with a complementary unmodified 12-mer oligodeoxynucleotide, it was found that there is a gradual increase in melting temperature with the number of ornithine residues, whereas the appended peptide did not perturb the B form of the duplex.
authors.affiliation: Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854.
authors.forename: T
authors.initials: T
authors.lastname: Zhu
authors.forename: C H
authors.initials: CH
authors.lastname: Tung
authors.forename: K J
authors.initials: KJ
authors.lastname: Breslauer
authors.forename: W A
authors.initials: WA
authors.lastname: Dickerhof
authors.forename: S
authors.initials: S
authors.lastname: Stein
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
chemical_list: D009838:Oligodeoxyribonucleotides; D010455:Peptides; C008973:polyornithine