title: Dynamic observations on NK cell activity and IL-2 and IFN gamma production in vitro in patients on maintenance hemodialysis.
abstract: In order to study the immune function of patients on maintenance hemodialysis (MHD), we assayed NK cell cytotoxicity against K562 targets in 40 patients on MHD, and the production of IL-2 and IFN in peripheral blood mononuclear cells (PBMC) after PHA stimulation, in contrast to those in normal controls. The results showed that NK cell activity and IL-2 and IFN levels were markedly lower in the patients than in the controls. After a single dialysis, NK cell activity as well as IL-2 and IFN levels were elevated to different extent. But there was no significant change in patients after long-term dialysis. There was a positive correlation between the NK cell activity and IL-2 and IFN activity in the controls, but no such correlation was found in the patients on MHD. There was a positive correlation between the NK cell activity and IL-2 activity in patients after dialysis, suggesting that immune function were impaired in the patients on MHD, with a decline in the activity of NK cell and IL-2 and IFN, and a disorder of immune regulation cycle. These abnormal immune impairments in the patients could be partly corrected by hemodialysis. However, long-term hemodialysis is not much helpful in the improvement of patient's immune function.
authors.affiliation: Department of Nephrology, Shenzhen People's Hospital.
authors.forename: Y
authors.initials: Y
authors.lastname: Dai
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: S C
authors.initials: SC
authors.lastname: Tang
authors.forename: T Y
authors.initials: TY
authors.lastname: Cai
authors.forename: D
authors.initials: D
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007676:Kidney Failure, Chronic / Q000276:immunology* / Q000628:therapy; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects
chemical_list: D007376:Interleukin-2; D007371:Interferon-gamma