In vitro modulation of interleukin-1 beta secretion by cultured rat doxorubicin-stimulated whole glomeruli and dissociated mesangial glomerular cells.
Doxorubicin-stimulated whole rat glomeruli and dissociated mesangial and resident glomerular macrophage cells produced the release of interleukin (IL)-1 beta cytokine. This activity increased after the addition of lipopolysaccharide (LPS) or LPS plus indomethacin to the cultures. In the presence of WEB2086 [platelet-activating factor (PAF)-acether antagonist], this activity showed a drastic reduction, without modification after sodium furegrelate (thromboxane synthetase inhibitor) was added to the cultures. Our results also demonstrate that this IL-1 beta activity is mainly produced by glomerular-resident macrophage cells. These findings support the important role by both IL-1 beta and PAF-acether mediator factors, at the cellular level, in the rat model of doxorubicin-induced nephrosis.
Bricio
T
T
Department of Pathology, Hospital Ramon y Cajal, Madrid, Spain.
Molina
A
A
Martin
A
A
Mampaso
F
F
D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D004347:Drug Interactions; D005920:Glomerular Mesangium / Q000187:drug effects / Q000276:immunology; D007375:Interleukin-1 / Q000737:chemistry / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000187:drug effects* / Q000276:immunology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008970:Molecular Weight; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley; D014230:Triazoles / Q000494:pharmacology
D001381:Azepines; D007375:Interleukin-1; D008070:Lipopolysaccharides; D010972:Platelet Activating Factor; D014230:Triazoles; C052518:WEB 2086; D004317:Doxorubicin