[Isolation, purification, and comparative study of the properties of adenosine deaminase from five regions of the cattle brain].
Adenosine deaminase from the white and gray matter of the large hemispheres, cerebellum, medulla oblongata and pituitary anterior lobe has been isolated and purified. The pH optimum, Km, molecular mass, yield and specific activities for all the enzyme preparations have been determined. Gel filtration and electrophoresis data point to the heterogeneity of the enzyme. The lack of effects of SH-reagents suggests the absence of an essential SH-group. Among five bivalent metal ions, only Cu2+ irreversibly inhibited the enzyme activity in all the preparations.
Sharoian
S G
SG
Antonian
A A
AA
Mardanian
S S
SS
D000243:Adenosine Deaminase / Q000302:isolation & purification* / Q000378:metabolism; D058892:Adenosine Deaminase Inhibitors; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002384:Catalysis; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D008970:Molecular Weight
D058892:Adenosine Deaminase Inhibitors; D008670:Metals; D000243:Adenosine Deaminase
Vydelenie, ochistka i sravnitel'noe izuchenie svoĭstv adenozindezaminazy iz piati otdelov mozga krupnogo rogatogo skota.