Solid-phase binding analysis of N-CAM interactions with brain fodrin.
The large cytoplasmic domain form of the neural cell adhesion molecule N-CAM has been reported to interact specifically with fodrin, a submembranous cytoskeletal protein. We tested the abilities of fodrins from bovine brain and embryonic chicken brain to bind to N-CAM that had been isolated from differentiated or undifferentiated mouse N2A neuroblastoma cells or from the brains of embryonic day 11 or day 14 chickens. Labeled fodrin samples bound with immobilized fodrin at a minimum soluble fodrin concentration of 2.5 x 10(-8) M, but the labeled fodrin did not bind to the immobilized N-CAM when incubated at 20-fold higher fodrin concentrations.
Woo
M K
MK
Department of Developmental and Cell Biology, University of California, Irvine 92717-2300.
Murray
B A
BA
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011485:Protein Binding; D013045:Species Specificity; D014407:Tumor Cells, Cultured; D013049:Spectrin
D002352:Carrier Proteins; D015816:Cell Adhesion Molecules, Neuronal; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D013049:Spectrin