title: Isolation, sequence and expression of a cDNA encoding the alpha-chain of the feline CD8.
abstract: We have cloned, sequenced and expressed a cDNA encoding the alpha-chain of feline CD8. This clone, named FT8-10, has an open reading frame with 720 nucleotides in length encoding a protein with 239 amino acid residues. Sequence analysis has revealed that the feline CD8 alpha-chain (CD8 alpha) shares significant homology with human (T8/Leu-2), bovine (BoCD8), rat (MRC OX8) and mouse (Lyt-2) CD8 alpha subunits. Cysteine residues as well as the tyrosine kinase p56lck binding site are well conserved. Besides, no putative N-linked glycosylation site was found. Interestingly, immunofluorescence analysis of COS-7 cells transfected with feline CD8 alpha expression plasmid driven by SR alpha promoter showed that the expressed feline CD8 alpha cross-reacted with an anti-human CD8 alpha monoclonal antibody OKT8, but did not react with an anti-feline monoclonal antibody, FT-2, which is thought to recognize the feline analogue of the human T8/Leu-2 and murine Lyt-2 molecules expressed on cytotoxic/suppressor T cells.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: M R
authors.initials: MR
authors.lastname: Pecoraro
authors.forename: Y
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: J
authors.initials: J
authors.lastname: Norimine
authors.forename: K
authors.initials: K
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Toyosaki
authors.forename: Y
authors.initials: Y
authors.lastname: Tohya
authors.forename: C
authors.initials: C
authors.lastname: Kai
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016827:CD8 Antigens / Q000235:genetics*; D002415:Cats / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D013045:Species Specificity
chemical_list: D016827:CD8 Antigens; D018076:DNA, Complementary