title: Diagnosis of infections with Shiga-like toxin-producing Escherichia coli by use of enzyme-linked immunosorbent assays for Shiga-like toxins on cultured stool samples.
abstract: Shiga-like toxin-producing (SLT) Escherichia coli, particularly those belonging to serogroup O157, are responsible for haemorrhagic colitis, haemolytic uraemic syndrome and some cases of gastro-enteritis. The rapid and reliable diagnosis of all these infections is necessary for correct patient management and for epidemiological reasons, but is rarely possible with present methods. We compared the efficacy of two methods, (i) the culture of faeces in broth that contained mitomycin C followed by enzyme-linked immunosorbent assay (ELISA) for SLTs, and (ii) the culture of faeces on sorbitol MacConkey agar (SMA), in the detection of infections caused by SLT-producing E. coli. SLT-producing E. coli O157 strains were isolated on SMA from 42 of 475 faecal samples, but SLTs were detected by ELISA in culture supernates or lysates of 54 of 475 samples. SLT-producing E. coli strains were isolated subsequently from 11 of 12 ELISA-positive, SMA culture-negative samples by a colony blot technique. In four cases, SLT-producing E. coli of serogroups other than O157 were isolated and in seven cases E. coli O157 was isolated in small numbers. The ELISA is a rapid and sensitive technique for the diagnosis of SLT-producing E. coli infection, especially where low numbers of the organism are present in faeces and when the infection is caused by a serogroup other than O157.
authors.affiliation: Department of Microbiology, Hope Hospital, Salford.
authors.forename: D
authors.initials: D
authors.lastname: Law
authors.forename: A A
authors.initials: AA
authors.lastname: Hamour
authors.forename: D W
authors.initials: DW
authors.lastname: Acheson
authors.forename: H
authors.initials: H
authors.lastname: Panigrahi
authors.forename: L A
authors.initials: LA
authors.lastname: Ganguli
authors.forename: D W
authors.initials: DW
authors.lastname: Denning
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis* / Q000096:biosynthesis; D003092:Colitis / Q000175:diagnosis / Q000382:microbiology; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D006471:Gastrointestinal Hemorrhage / Q000175:diagnosis / Q000382:microbiology; D006463:Hemolytic-Uremic Syndrome / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2
chemical_list: D001427:Bacterial Toxins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2