Detection of neurotensin in tissues by capillary zone electrophoresis.
An electrophoretic method for detecting neurotensin in tissues was developed. Homogenates of rat duodenum and adrenal glands were first extracted by solid-phase extraction and purified by reversed-phase high-performance liquid chromatography. The neurotensin-rich fraction was further analyzed by capillary zone electrophoresis (CZE) using a commercial instrument with UV detection. A minor peak was detected as neurotensin and its identity was further confirmed by performing several CZE analyses at different pH values. Such an approach could be used to analyze with good molecular specificity the neuropeptides in some human tissues.
Denoroy
L
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Département de Médecine Expérimentale INSERM U52, CNRS URA 1195, Université Claude Bernard, Lyon, France.
D000311:Adrenal Glands / Q000737:chemistry; D000818:Animals; D002197:Capillary Action; D002851:Chromatography, High Pressure Liquid; D004386:Duodenum / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008297:Male; D009496:Neurotensin / Q000032:analysis*; D051381:Rats
D009496:Neurotensin