[Interaction of immobilized lectin from Leucojum vernum L. with polysaccharides and glycoproteins].
Interaction of D-mannose-specific lectin from Leucojum vernum L. with polysaccharides and glycoproteins has been studied on the column of immobilized lectin and by precipitation. Lectin is found to precipitate human and bovine thyroglobulin, yeast mannan, mannofucogalactans from Aphyllophores mushrooms and rystomycin sulfate. Lectin does not interact with yeast glycogen and gel sephadex and this differentiates it from Leucojum vernum L., Concanavalin A and pea lectin. All glycoconjugates are divided into three groups: those which are not bound with immobilized lectin; partly bound with Leucojum vernum lectin and completely bound with the column. Glycoconjugates that are partly bound with immobilized lectin are, probably, glycosyl moiety. The obtained results are discussed.
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D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation; D002847:Chromatography, Agarose; D003208:Concanavalin A; D005685:Galactans / Q000737:chemistry; D005947:Glucose; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry; D008358:Mannose; D037121:Plant Lectins; D011134:Polysaccharides / Q000737:chemistry*; D012310:Ristocetin / Q000737:chemistry; D013954:Thyroglobulin / Q000737:chemistry
D005685:Galactans; D006023:Glycoproteins; D037102:Lectins; D008351:Mannans; D037121:Plant Lectins; D011134:Polysaccharides; C036292:pea lectin; D003208:Concanavalin A; D012310:Ristocetin; D013954:Thyroglobulin; D005947:Glucose; D008358:Mannose
Vzaiemodiia imobilizovanoho lektynu bilosnizhky vesnianoï z polisakharydamy ta glikoproteïnamy.