Discrimination of fluorinated uridine metabolites in N-417 small cell lung cancer cell extracts via 19F- and 31P-NMR.
31P-NMR extract spectra of N-417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP-glucose, FUDP-glucuronate, FUDP-GlcNAc, and FUDP-GalNAc via enzymatic conversion and 19F- and 31P-NMR analysis. Distinct 19F chemical shifts were assigned for FUTP, FUDP, and the FUDP-sugars.
Pederson
N V
NV
Chemical Engineering Department, Northwestern University, Evanston, Illinois.
Zanghi
J A
JA
Miller
W M
WM
Knop
R H
RH
D018288:Carcinoma, Small Cell / Q000378:metabolism*; D005461:Fluorine; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010758:Phosphorus; D014407:Tumor Cells, Cultured; D014529:Uridine / Q000378:metabolism*; D014530:Uridine Diphosphate / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D014539:Uridine Diphosphate Sugars / Q000378:metabolism; D014544:Uridine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism
D014539:Uridine Diphosphate Sugars; D014535:Uridine Diphosphate Glucuronic Acid; D010758:Phosphorus; D005461:Fluorine; C025635:5-fluorouridine 5'-triphosphate; D014537:Uridine Diphosphate N-Acetylglucosamine; D014530:Uridine Diphosphate; D014536:Uridine Diphosphate N-Acetylgalactosamine; D014544:Uridine Triphosphate; D014532:Uridine Diphosphate Glucose; D014529:Uridine