title: The intrinsic 22 kDa protein is a chlorophyll-binding subunit of photosystem II.
abstract: The intrinsic 22 kDa polypeptide associated with photosystem II (psbS protein) was found to be able to bind chlorophyll. Extraction of isolated photosystem II membranes with octyl-thioglucopyranoside, followed by repetitive electrophoresis under partially denaturing conditions gave only one green band. It contained both chlorophyll a and chlorophyll b, exhibited an absorption maximum at 674 nm and a 77 K fluorescence peak at 675 nm. The chlorophyll-protein band contained a single polypeptide of 22 kDa. Based on these results and on previous protein sequence comparisons, it is suggested that the psbS protein is a chlorophyll a/b binding polypeptide and should thus be denoted CP22.
authors.affiliation: Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Funk
authors.forename: W P
authors.initials: WP
authors.lastname: Schröder
authors.forename: B R
authors.initials: BR
authors.lastname: Green
authors.forename: G
authors.initials: G
authors.lastname: Renger
authors.forename: B
authors.initials: B
authors.lastname: Andersson
mesh_terms: D002734:Chlorophyll / Q000378:metabolism*; D045342:Light-Harvesting Protein Complexes; D008970:Molecular Weight; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis
chemical_list: D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D002734:Chlorophyll