title: Inhibitory effect of deferoxamine or macrophage activation on transformation of Paracoccidioides brasiliensis conidia ingested by macrophages: reversal by holotransferrin.
abstract: Conidia of P. brasiliensis ingested by murine macrophages at 37 degrees C showed enhanced transformation to yeast cells and further intracellular growth compared with conidia in culture medium alone. Treatment of macrophages with the iron chelator deferoxamine inhibited the intracellular conidium-to-yeast transformation. Cytokine-activated macrophages could also exert this inhibitory effect. Holotransferrin reversed the inhibitory effect of either deferoxamine or activated macrophages on intracellular conidium-to-yeast transformation. These results indicate that iron restriction is one of the mechanisms by which activated macrophages control the intracellular transformation of ingested conidia and growth of yeast cells.
authors.affiliation: Corporacion para Investigaciones Biologicas, Hospital Pablo Tobon Uribe, Medellin, Colombia.
authors.forename: L E
authors.initials: LE
authors.lastname: Cano
authors.forename: B
authors.initials: B
authors.lastname: Gomez
authors.forename: E
authors.initials: E
authors.lastname: Brummer
authors.forename: A
authors.initials: A
authors.lastname: Restrepo
authors.forename: D A
authors.initials: DA
authors.lastname: Stevens
mesh_terms: D000818:Animals; D003676:Deferoxamine / Q000494:pharmacology*; D007501:Iron / Q000502:physiology*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010228:Paracoccidioides / Q000254:growth & development*; D011990:Receptors, Transferrin / Q000502:physiology; D014168:Transferrin / Q000494:pharmacology*
chemical_list: D011990:Receptors, Transferrin; D014168:Transferrin; D007501:Iron; D003676:Deferoxamine