Assessment of brain muscarinic acetylcholinergic receptors in living mice using a simple probe, [125I]-4-iododexetimide and [125I]-4-iodolevetimide.
This study describes assessment of brain muscarinic acetylcholinergic receptors in living mice using a single-crystal radiation detection system, the high-affinity antagonist [125I]-4-iododexetimide, and the inactive enantiomer [125I]-4-iodolevetimide. Kinetics of radioligand binding, as well as perturbation by atropine displacement, can be determined using this simple probe technique.
Sasaki
M
M
Department of Environmental Health Sciences, Johns Hopkins Medical Institutions, Baltimore, MD 21205-2179.
Müller-Gärtner
H W
HW
Lever
J R
JR
Ravert
H T
HT
Dannals
R F
RF
Guilarte
T R
TR
Wagner
H N
HN
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D003909:Dexetimide / Q000031:analogs & derivatives* / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011869:Radioligand Assay; D011976:Receptors, Muscarinic / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D007457:Iodine Radioisotopes; D011976:Receptors, Muscarinic; C066812:4-iododexetimide; D003909:Dexetimide; D001285:Atropine