[The possibilities of improving the serological diagnosis of active tuberculosis by using the technics of immunofluorescence and ELISA].
A group of 188 adult subjects has been studied as follows: 119 cases of pulmonary tb confirmed by smear and/or culture; 44 cases with other chronic lung diseases; 25 healthy persons. The following serological tests have been performed: immunofluorescence indirect test (FA) using mycobacterial total antigen; ELISA test using two antigens: PPD and a freeze dried crude sonicated extract from H37Rv strain (antigen "Prague"). The results show: sensitivity 77% for FA and 71% for ELISA; specificity 81% for FA and 86% for ELISA. If all positive results (to one or both tests) are being considered the sensitivity increases to 81% but specificity decreases to 77%. The ELISA test with both antigens succeeds in a good discrimination between lung active tb on one side and lung non-tb cases and healthy persons on the other side (p < 0.001). Using comparatively the two antigens in ELISA test a higher serological confirmation of active tb is reached with "Prague" antigen: 72.7% vs 62.8% with PPD (p < 0.025).
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Laboratorul de imunomicrobiologie, Institutul de Pneumoftiziologie M. Nasta, Bucureşti.
Algeorge
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D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D008171:Lung Diseases / Q000175:diagnosis; D009169:Mycobacterium tuberculosis / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D000907:Antibodies, Bacterial
Posibilităţi de perfecţionare a diagnosticului serologic al tuberculozei active prin aplicarea technicilor de imunofluorescenţă şi ELISA.