title: Critical amino acids responsible for converting specificities of proteins and for enhancing enzyme evolution are located around beta-turn potentials: data-based prediction.
abstract: Various reports have described that amino acid substitutions can alter substrate, positional, inhibitory, and target gene specificities of proteins. By using the method of Chou and Fasman, the present work predicts that critical amino acids for converting these substrate specificities of trypsin, L-lactate dehydrogenase, aspartate aminotransferase, beta-lactamase, and cytochrome P-450 are found to exist within regions predicted as beta-turns. The ratios of hydroxylation and oxygenation positions of substrates by cytochrome P-450 and lipoxygenase, respectively, are varied by changes of the protein structures, probably around turn conformations. Inhibitory specificities of bovine pancreatic trypsin inhibitor and alpha 1-antitrypsin and target gene specificity of glucocorticoid receptor are converted by changing turn structures. Occurrence of beta-turn probabilities can be predicted around the amino acid alteration positions of an evolutionally antecedent protein of a nylon degradation enzyme. These findings will have relevance to work on protein engineering and enzyme evolution.
authors.affiliation: Musashigaoka College, Saitama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Murakami
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids; D001219:Aspartate Aminotransferases / Q000737:chemistry / Q000378:metabolism; D005075:Biological Evolution; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D007256:Information Systems; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism; D001618:beta-Lactamases / Q000737:chemistry / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004798:Enzymes; D011506:Proteins; D003577:Cytochrome P-450 Enzyme System; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D014357:Trypsin; D001618:beta-Lactamases