Properties and applications of a new type of iodinated gradient medium.
This paper reports on the properties of a new iodinated density-gradient medium, Iotrolan, and its potential application for separating dense cells. Iotrolan is a dimeric, nonionic, iodinated compound which is distinguished by its ability to form dense, non-viscous gradients of low osmolarity. Isotonic gradients of up to 1.30 g/ml can be formed, allowing even dense cells such as sperm to be separated under isotonic conditions.
Bendixen
B
B
Department of Biology, University of Essex, Colchester, UK.
Rickwood
D
D
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival; D002499:Centrifugation, Density Gradient / Q000379:methods; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003124:Colorimetry; D003287:Contrast Media / Q000737:chemistry*; D006801:Humans; D007472:Iohexol / Q000737:chemistry; D007485:Ioxaglic Acid / Q000737:chemistry; D008297:Male; D009994:Osmolar Concentration; D012031:Refractometry; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013094:Spermatozoa / Q000166:cytology; D014283:Triiodobenzoic Acids / Q000737:chemistry*
D003287:Contrast Media; D014283:Triiodobenzoic Acids; C033300:iotrolan; D007472:Iohexol; D007485:Ioxaglic Acid