Influence of cyclic AMP on the biosynthesis of phosphatidylcholine in Microsporum gypseum.
The influence of intracellular cAMP on phosphatidylcholine biosynthesis in Microsporum gypseum has been examined using radiolabelled precursors. The incorporation of labelled choline, methionine and ethanolamine into total lipids, phospholipids and choline containing phospholipids increased in aminophylline and decreased in atropine grown cells as a result of rise and fall in cAMP levels in these cells. The enhanced uptake of labelled methionine and ethanolamine in comparison to labelled choline in choline containing phospholipids in aminophylline grown cells suggests that methylation pathway is more influenced by cAMP than CDP-choline pathway.
Bindra
A
A
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh.
Khuller
G K
GK
D000628:Aminophylline / Q000494:pharmacology; D001285:Atropine / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002794:Choline / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D003566:Cytidine Diphosphate Choline / Q000378:metabolism; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D008864:Microsporum / Q000187:drug effects / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis*
D002250:Carbon Radioisotopes; D004983:Ethanolamines; D010713:Phosphatidylcholines; D000628:Aminophylline; D003566:Cytidine Diphosphate Choline; D019856:Ethanolamine; D001285:Atropine; D008715:Methionine; D000242:Cyclic AMP; D002794:Choline