Transfusions to group O subjects of 2 units of red cells enzymatically converted from group B to group O.
BACKGROUND
It has previously been shown that full-unit (200 mL) transfusions of red cells (RBCs) enzymatically converted from group B to group O by treatment with alpha-galactosidase (ECO RBCs) are both safe and efficacious for normal group O or A subjects.


METHODS
The present study describes the results of a comprehensive clinical and serologic assessment of 2-unit (400 mL) ECO RBC transfusions to each of four normal group O subjects (after each had donated 1 unit of whole blood).


RESULTS
Clinical (hematologic tests, chemistry analysis, urinalysis) and serologic analyses revealed no evidence of immediate or delayed transfusion reaction, despite a threefold to fivefold elevation in pre-existing anti-B antiglobulin titer. 51Cr-labeled ECO RBCs were administered to one of the four subjects to allow direct measurement of ECO RBC survival in the circulation, which indicated that it was normal (24-hour survival, 95%; t1/2, 29.5 days). The observed increases in hemoglobin (by 1.3 +/- 0.4 g/dL [13 +/- 4 g/L]) and hematocrit (by 3.2 +/- 0.8% [0.032 +/- 0.008]) in transfused subjects provide further evidence of the efficacy of these cells in vivo.


CONCLUSIONS
These results extend those observed in our earlier 1-unit transfusion studies and suggest that ECO RBCs pose little risk and will be useful in transfusion medicine.
Lenny
L L
LL
Cell Biochemistry Laboratory, Lindsley F. Kimball Research Institute of The New York Blood Center, New York.
Hurst
R
R
Goldstein
J
J
Galbraith
R A
RA
D000017:ABO Blood-Group System / Q000276:immunology*; D000328:Adult; D000954:Antigens, Surface / Q000097:blood*; D001787:Blood Group Incompatibility / Q000517:prevention & control*; D001788:Blood Grouping and Crossmatching; D001803:Blood Transfusion; D002860:Chromium Radioisotopes; D003298:Coombs Test; D004905:Erythrocyte Aging; D005260:Female; D006801:Humans; D008297:Male; D000519:alpha-Galactosidase / Q000378:metabolism*
D000017:ABO Blood-Group System; D000954:Antigens, Surface; D002860:Chromium Radioisotopes; D000519:alpha-Galactosidase