Immunogenicity in guinea-pigs of a crude ribosomal fraction from Microsporum canis.
The immunogenicity of a crude ribosomal fraction (CRF) extracted from Microsporum canis was tested by assessing protection of vaccinated guinea-pigs (GP) against a challenge with the dermatophyte. Stimulation of the humoral as well as the cellular immune systems of these animals was evaluated by ELISA and the lymphocyte stimulation tests, respectively. In addition, the immune response elicited by the M. canis CRF was examined for cross-reactivity towards Trichophyton verrucosum antigen. The duration of the infection induced by M. canis in GP was reduced to 8 days in vaccinated animals versus 37 days in the control GP. Both humoral and cellular immune systems were stimulated by the CRF. Sera of GP vaccinated with M. canis CRF revealed presence of anti-T. verrucosum antibodies, albeit at titres significantly lower than against M. canis.
Elad
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Department of Bacteriology, Kimron Veterinary Institute, Beit-Dagan, Israel.
Segal
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E
D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis; D000946:Antigens, Fungal / Q000008:administration & dosage* / Q000302:isolation & purification; D003429:Cross Reactions; D003881:Dermatomycoses / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D005657:Fungal Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D006168:Guinea Pigs; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008864:Microsporum / Q000276:immunology*; D012270:Ribosomes / Q000276:immunology
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D005657:Fungal Vaccines