The effect of suramin on vasodilator responses to ATP and 2-methylthio-ATP in the Sprague-Dawley rat coronary vasculature.
The effect of suramin, a P2 purinoceptor antagonist, on the vasodilator response to adenosine 5'-triphosphate (ATP), 2-methylthio-ATP (2-meSATP) and adenosine were examined in the Sprague-Dawley rat coronary vasculature using the Langendorff heart preparation. Relaxation induced by 2-meSATP was significantly inhibited by suramin. Only responses to low doses of adenosine and ATP were inhibited by suramin. 8-(p-Sulphophenyl)theophylline (8-PSPT) did not affect the relaxant response to ATP and 2-meSATP at a concentration that significantly inhibited the response to adenosine. It is concluded that 2-meSATP acts via P2Y purinoceptors while ATP appears to be acting largely through a different mechanism. It is not acting via a P1 purinoceptor because ATP was not inhibited by the P1 purinoceptor antagonist 8-PSPT.
Vials
A J
AJ
Department of Anatomy and Developmental Biology, University College London, UK.
Burnstock
G
G
D000241:Adenosine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D003331:Coronary Vessels / Q000187:drug effects* / Q000502:physiology*; D004347:Drug Interactions; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013498:Suramin / Q000494:pharmacology*; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology; D013873:Thionucleotides / Q000494:pharmacology*; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology; D014665:Vasodilator Agents / Q000494:pharmacology*
D013873:Thionucleotides; D014665:Vasodilator Agents; D013498:Suramin; C031433:8-(4-sulfophenyl)theophylline; D000255:Adenosine Triphosphate; D013806:Theophylline; D000241:Adenosine; C008501:2-methylthio-ATP