Regulation of glycogen synthase activity in isolated rat adipocytes by levamisole.
The effect of levamisole on glycogen synthase activity in isolated adipocytes was studied. The addition of levamisole to these cells resulted in an acute concentration-dependent increase in glycogen synthase activity. In contrast, epinephrine, dibutyryl cyclic AMP (DcAMP) and cysteamine decreased glycogen synthase activity. The stimulatory effect of levamisole on the activity of the enzyme was not affected by the presence of epinephrine but was diminished when either DcAMP or cysteamine was present. The results of this study suggest that levamisole increases adipocyte glycogen synthase activity by a mechanism that can be reversed by the elevation of cAMP.
Basi
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Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, DC 20059.
George
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Pointer
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D017667:Adipocytes / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D003543:Cysteamine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006006:Glycogen Synthase / Q000187:drug effects* / Q000378:metabolism; D007978:Levamisole / Q000494:pharmacology*; D008297:Male; D051381:Rats
D007978:Levamisole; D003543:Cysteamine; D003994:Bucladesine; D000242:Cyclic AMP; D006006:Glycogen Synthase; D004837:Epinephrine