title: The hepatic receptor for sex steroid-binding protein: study on a non-malignant cell line (Chang liver).
abstract: The binding of human sex steroid-binding protein (SBP), labelled with 125I, was studied on whole cultured liver cells (Chang liver cells). SBP was shown to bind to a receptor site on normal hepatocytes. The binding was time- and temperature-dependent, highly specific and of high affinity. The liver receptor recognizing SBP was demonstrated to be different from the asialoglycoprotein receptor; in addition, laminin, which is structurally related to SBP, could not bind to the receptor. SBP was shown to recognize two binding sites with different affinities; the low affinity site was shown to possess a remarkably high capacity. This characteristic, which until now has been described only for hepatocytes, could be related to the unique role of the liver with regard to both SBP and sex steroids.
authors.affiliation: Dipartimento di Fisiopatologia Clinica, Università di Torino, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Fortunati
authors.forename: M
authors.initials: M
authors.lastname: Becchis
authors.forename: F
authors.initials: F
authors.lastname: Fissore
authors.forename: L
authors.initials: L
authors.lastname: Berta
authors.forename: M G
authors.initials: MG
authors.lastname: Catalano
authors.forename: M
authors.initials: M
authors.lastname: Orsello
authors.forename: G
authors.initials: G
authors.lastname: Gaidano
authors.forename: R
authors.initials: R
authors.lastname: Frairia
mesh_terms: D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins / Q000378:metabolism; D001667:Binding, Competitive; D001798:Blood Proteins / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D007700:Kinetics; D007797:Laminin / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism*; D013696:Temperature
chemical_list: D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D001798:Blood Proteins; D007797:Laminin; D011956:Receptors, Cell Surface; D012738:Sex Hormone-Binding Globulin; C063572:sex hormone-binding globulin receptor