Cellular sources of proteolytic enzymes in equine joints.
Isolated equine blood and articular cells were investigated for proteolytic enzyme production by means of gel filtration and analysis on 14C-acetylated collagen and casein substrates. Significant amounts of collagenase and caseinase activity were produced by cultured synoviocytes stimulated with equine interleukin 1, although large amounts of collagenase also originated from neutrophils.
Spiers
S
S
Division of Equine Studies, University of Liverpool, Leahurst, Neston, South Wirral, UK.
May
S A
SA
Bennett
D
D
Edwards
G B
GB
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000201:enzymology*; D017364:Collagenases / Q000032:analysis* / Q000097:blood; D006736:Horses / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology; D008666:Metalloendopeptidases; D009000:Monocytes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology; D010447:Peptide Hydrolases / Q000032:analysis* / Q000097:blood; D013582:Synovial Fluid / Q000201:enzymology; D013583:Synovial Membrane / Q000187:drug effects / Q000201:enzymology*
D007375:Interleukin-1; D010447:Peptide Hydrolases; D017364:Collagenases; D008666:Metalloendopeptidases; C030895:caseinase