title: A type 1 DNA topoisomerase from the kinetoplast hemoflagellate Leishmania donovani.
abstract: A type 1 DNA topoisomerase has been purified from the nuclei of the kinetoplast hemoflagellate Leishmania donovani using polyethylene glycol fractionation and chromatography on hydroxylapatite, phosphocellulose and phenylsepharose column. The relaxation activity is ATP independent. Mg2+ is an essential cofactor for the reaction with an optimum at 10 mM. Mg2+ can be substituted by Mn2+ at 5 mM concentration. The relaxation reaction exhibits a salt optimum at 100 mM KCl. The enzyme can not remove supercoils from positive superhelical DNAs nor can induce supercoiling of relaxed DNAs. The topoisomerase activity is associated with a polypeptide of molecular weight about 67 kDa as shown by sephacryl-S200 gel filtration and by electrophoresis on sodium dodecyl sulphate-polyacrylamide gels.
authors.affiliation: Molecular Parasitology Laboratory, Indian Institute of Chemical Biology, Calcutta.
authors.forename: A K
authors.initials: AK
authors.lastname: Chakraborty
authors.forename: A
authors.initials: A
authors.lastname: Gupta
authors.forename: H K
authors.initials: HK
authors.lastname: Majumder
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification* / Q000378:metabolism; D018105:DNA, Kinetoplast / Q000378:metabolism; D017886:Durapatite; D007700:Kinetics; D007893:Leishmania donovani / Q000201:enzymology*; D011092:Polyethylene Glycols
chemical_list: D018105:DNA, Kinetoplast; D011092:Polyethylene Glycols; D017886:Durapatite; D004264:DNA Topoisomerases, Type I