title: In vitro adsorption of dichlorvos and parathion by activated charcoal.
abstract: Accidental and suicidal ingestions of organophosphate compounds continue to be a common occurrence in Turkey. Activated charcoal administration without gastric emptying has been advocated as primary therapy in most acute poisoning cases, although some references do not recommend activated charcoal use in organophosphate poisoning. This study was performed to determine the in vitro adsorption of dimethyl dichlorovinyl phosphate (dichlorvos) and parathion by activated charcoal over a wide range of charcoal:organophosphate ratios (1:1, 2.5:1, 5:1, 10:1 and 20:1, g:g). The charcoal binding ability of dichlorvos and parathion were studied in both pH 1.2 and pH 7 environments. The supernatant was extracted with n-hexane and then analyzed by gas chromatography. Each incremental increase in charcoal dose increased the percent adsorption of dichlorvos and parathion. At the 20:1 ratio, 82.8 +/- 2.0/87.3 +/- 2.9% (pH 1.2/7.0) of dichlorvos and 59.3 +/- 4.5/64.5 +/- 6.1% (pH 1.2/7.0) of parathion were bound by activated charcoal. There were no significant differences in amounts of compound bound in the acid and neutral solutions. Large doses of activated charcoal effectively bind dichlorvos and parathion in vitro. In vivo research should be performed to determine activated charcoal's role in organophosphate poisoning cases.
authors.affiliation: Dokuz Eylul University Hospital, Izmir, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Guven
authors.forename: Y
authors.initials: Y
authors.lastname: Tuncok
authors.forename: S
authors.initials: S
authors.lastname: Gidener
authors.forename: A
authors.initials: A
authors.lastname: Gelal
authors.forename: M
authors.initials: M
authors.lastname: Demetci
authors.forename: J
authors.initials: J
authors.lastname: Fowler
authors.forename: S
authors.initials: S
authors.lastname: Apaydin
authors.forename: M
authors.initials: M
authors.lastname: Keskin
mesh_terms: D000327:Adsorption; D002606:Charcoal / Q000378:metabolism / Q000494:pharmacology*; D004006:Dichlorvos / Q000378:metabolism / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D008954:Models, Biological; D010278:Parathion / Q000378:metabolism / Q000493:pharmacokinetics*
chemical_list: D002606:Charcoal; D010278:Parathion; D004006:Dichlorvos