Purification and partial sequence of proteins involved in the cholic acid transport into rat liver hepatocytes.
Two proteins, in previous work labeled by affinity markers derived from taurocholic acid, were purified and partially sequenced. Antibodies were raised against purified proteins, and cross-reactions were carefully checked. The influence of these antibodies upon taurocholic acid import into vesicles from rat liver plasma membranes was measured, and showed a distinct inhibition of transport in the case of the 54 kD protein.
Hoffmann
A
A
Biozentrum Frankfurt, Abt. Biochemie, N 210/220, Germany.
Müllner
S
S
Ziegler
K
K
Fasold
H
H
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002462:Cell Membrane; D019826:Cholic Acid; D002793:Cholic Acids / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D013656:Taurocholic Acid / Q000378:metabolism
D000345:Affinity Labels; D002352:Carrier Proteins; D002793:Cholic Acids; D008565:Membrane Proteins; D013656:Taurocholic Acid; D019826:Cholic Acid