HLA-DP does not contribute towards susceptibility to systemic lupus erythematosus.
OBJECTIVE
To determine whether HLA-DP genes are involved in determining susceptibility to systemic lupus erythematosus (SLE).


METHODS
HLA-DPA1 and DPB1 genes were amplified by PCR of DNA samples from a panel of patients with SLE and normal controls. Amplified DNA was blotted on to nylon filters and probed with sequence-specific oligonucleotide (SSO) probes.


RESULTS
No DPA1 or DPB1 allele was significantly associated with SLE, or with any immunological or clinical subset of SLE. Evidence was found for only limited linkage disequilibrium between HLA-DP and HLA-DQ/DR variants, and none between HLA-DP and the TAP2 gene.


CONCLUSIONS
These data indicate that HLA-DP genes do not contribute towards determining susceptibility to SLE.
Davies
E J
EJ
University of Manchester, Rheumatic Diseases Centre, Hope Hospital, Salford, United Kingdom.
Hutchings
C J
CJ
Hillarby
M C
MC
Donn
R P
RP
Cooper
R G
RG
Hay
E M
EM
Bernstein
R M
RM
Holt
P J
PJ
Grennan
D M
DM
Ollier
W E
WE
D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001323:Autoantibodies / Q000032:analysis; D002648:Child; D004198:Disease Susceptibility; D005260:Female; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006682:HLA-DP Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged
D001323:Autoantibodies; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens