Effects of eosinophilotropic cytokines on differentiation of an eosinophil cell line, YY-1.
Differentiation of an eosinophilic cell line, YY-1, was induced by treatment with 0.3 mM butyric acid (BA). The effects of eosinophilotropic cytokines, such as IL-3, IL-5, and GM-CSF on BA-induced differentiation, especially in the chemotactic response to ECF, was examined. Five STO-2-derived ECFs, IL-5, C5a, and fMLP were used as eosinophil chemoattractants. The effects of the cytokines on the chemotactic response of YY-1 greatly differed according to the ECF used. It was thus suggested that heterogeneity in the chemotactic response of eosinophils depends on the cytokines which act on eosinophils during differentiation.
Ueno
M
M
Department of Pathology, Kumamoto University Medical School, Japan.
Watanabe
K
K
Tsurufuji
S
S
Hirashima
M
M
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002631:Chemotactic Factors, Eosinophil / Q000494:pharmacology*; D004804:Eosinophils / Q000166:cytology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D015848:Interleukin-5 / Q000494:pharmacology*
D002631:Chemotactic Factors, Eosinophil; D007377:Interleukin-3; D015848:Interleukin-5; D016178:Granulocyte-Macrophage Colony-Stimulating Factor