Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations.
Immunomagnetic sorting, sequential filtrations, and counterflow centrifugal elutriation were compared for their ability to obtain enriched populations of Pneumocystis carinii developmental stages from infected rat-lung homogenates. Elutriation combined with sequential filtrations resulted in highly (> 95%) enriched populations of P. carinii cysts and trophozoites with excellent viability. This approach offers advantages over previously described methods of obtaining enriched P. carinii cell populations and should have important applications to research on this organism.
De Stefano
J A
JA
Complex Carbohydrate Research Center, University of Georgia, Athens 30602.
Foy
J M
JM
Sullivan
D W
DW
Dawes
S M
SM
Cushion
M T
MT
Babcock
G F
GF
Sleight
R G
RG
van Halbeek
H
H
Walzer
P D
PD
D000818:Animals; D002498:Centrifugation; D005374:Filtration; D008168:Lung / Q000382:microbiology; D008280:Magnetics; D008297:Male; D009172:Mycology / Q000379:methods; D011010:Pneumocystis / Q000254:growth & development* / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology*; D051381:Rats; D017207:Rats, Sprague-Dawley