2-Hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine induction of recombinational mutations in mammalian cell lines as detected by DNA fingerprinting.
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant mutagenic heterocyclic amine in cooked foods. Two mouse tumor cell lines, BMT11 and FM3A, were exposed to its proximate form, 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine (N-OH-PhIP). Fifty-six subclones of BMT11 and 39 subclones of FM3A were isolated and analyzed by DNA fingerprinting. Treatment with 10-20 microM N-OH-PhIP gave rise to extra bands or shifted bands, but treatment without N-OH-PhIP did not. This suggests that mutations resulting from recombination were induced. The mutation frequencies were 21-53% and 22-35% for BMT11 and FM3A, respectively. These findings suggest that PhIP induces recombinational mutations.
Kitazawa
T
T
First Department of Biochemistry, Niigata University School of Medicine, Japan.
Kominami
R
R
Tanaka
R
R
Wakabayashi
K
K
Nagao
M
M
D000818:Animals; D001483:Base Sequence; D002999:Clone Cells; D004249:DNA Damage; D016172:DNA Fingerprinting; D004273:DNA, Neoplasm / Q000032:analysis / Q000187:drug effects; D007093:Imidazoles / Q000633:toxicity*; D008325:Mammary Neoplasms, Experimental / Q000235:genetics; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation / Q000235:genetics*; D009374:Neoplasms, Experimental / Q000235:genetics*; D011725:Pyridines / Q000633:toxicity*; D011995:Recombination, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004273:DNA, Neoplasm; D007093:Imidazoles; D009153:Mutagens; D011725:Pyridines; C060533:2-hydroxyamino-1-methyl-6-phenylimidazo(4,5-b)pyridine