Na+,K(+)-ATPase activity of rabbit kidney cortex membranes in ischemia and reperfusion.
The activity of Na+,K(+)-ATPase in the microsomal fraction of rabbit kidney cortex was strongly decreased by ischemia and increased slightly, but not significantly, after reperfusion. These changes were correlated with a dramatic increase in lipid peroxidation in microsomes isolated from both ischemic and reperfused kidneys. This correlation may point to irreversible impairment of the enzymatic function under the influence of either oxygen free radicals or lipid peroxidation.
Aydin
S
S
Ministry of Health, Ankara Training Hospital, Department of Biochemistry, Cebeci, Turkey.
Aricioglu
A
A
Turkozkan
N
N
Bingol
F
F
Aydin
C
C
D000818:Animals; D005609:Free Radicals; D007511:Ischemia / Q000201:enzymology*; D007672:Kidney Cortex / Q000098:blood supply* / Q000201:enzymology* / Q000293:injuries; D015227:Lipid Peroxidation; D008297:Male; D008861:Microsomes / Q000201:enzymology; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D005609:Free Radicals; D017382:Reactive Oxygen Species; D000254:Sodium-Potassium-Exchanging ATPase