title: Myofibroblast and concurrent ED-B fibronectin phenotype in human stromal cells cultured from non-malignant and malignant breast tissue.
abstract: Primary cultures of stromal cells from non-malignant and malignant breast tissues contained myofibroblasts based on immunoreactivity to alpha-smooth muscle (alpha-sm) actin. The proportions of these cells were variable among cultures from non-malignant origin while consistently high in cultures from carcinomas. High expression of ED-B fibronectin and of type V collagen was observed in myofibroblast-containing cultures. While cells from non-malignant tissues grew relatively steadily, the proliferation of carcinoma-derived cells declined during serial subculturing. In both types of cultures, alpha-sm actin and ED-B fibronectin expression decreased with increasing passage numbers. Epidermal growth factor (EGF), fibroblast growth factor b (FGFb), transforming growth factor alpha (TGF alpha) and platelet-derived growth factor (PDGF) showed consistent mitogenic effects. Addition of FGFb prolonged culture growth and allowed alpha-sm actin and ED-B fibronectin expression to persist. These results demonstrate similar phenotypic modulations in stromal cells from non-malignant and malignant breast tissues that may reflect a common stromal response to various tissue injuries, including neoplasia.
authors.affiliation: Institut d'Oncologie Cellulaire et Moléculaire Humaine, Bobigny, France.
authors.forename: D
authors.initials: D
authors.lastname: Brouty-Boyé
authors.forename: V
authors.initials: V
authors.lastname: Magnien
mesh_terms: D000199:Actins / Q000032:analysis*; D001940:Breast / Q000737:chemistry*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000473:pathology; D005353:Fibronectins / Q000032:analysis*; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D017154:Stromal Cells / Q000737:chemistry; D014407:Tumor Cells, Cultured
chemical_list: D000199:Actins; D005353:Fibronectins; D006133:Growth Substances