Micellar electrokinetic chromatography of hydroxyproline and other secondary amino acids in biological samples with laser-induced fluorescence detection.
Micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence (LIF) was used for the rapid and sensitive detection of hydroxyproline in serum and hydrolyzed urine that were pre-column derivatized with 9-fluorenylmethyl chloroformate (FMOC). The application of the combined o-phthalaldehyde (OPA)/FMOC derivatization in MEKC for the selective detection of secondary amino acids in biological samples is investigated.
Chan
K C
KC
PRI/DynCorp, NCI Frederick Cancer Research and Development Center, MD 21702.
Janini
G M
GM
Muschik
G M
GM
Issaq
H J
HJ
D000596:Amino Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D002845:Chromatography; D004563:Electrochemistry; D004586:Electrophoresis; D005449:Fluorenes; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis* / Q000097:blood / Q000652:urine; D007202:Indicators and Reagents; D007834:Lasers; D008823:Micelles; D013050:Spectrometry, Fluorescence; D009764:o-Phthalaldehyde
D000596:Amino Acids; D005449:Fluorenes; D007202:Indicators and Reagents; D008823:Micelles; D009764:o-Phthalaldehyde; C054007:1-(9-fluorenyl)methyl chloroformate; D006909:Hydroxyproline