Folding of barnase in parts.
Stretches of residual structure in the unfolded states of proteins could possibly constitute crucial regions that initiate protein folding. We are searching for such regions in barnase by dividing it into fragments. By this means, we can search for regions that just form within local sequences. We are also employing methods that can detect low levels of residual structure. In this study, we examine the fragment 1-22 and a large fragment (23-110) that contains all of the catalytic residues. Fragment 1-22 contains the first alpha-helix, and fragment 23-110 contains the second alpha-helix and beta-sheet structure-forming residues of native barnase. These fragments bind together rapidly and tightly upon association to form a fully native-like complex. Studies by circular dichroism and fluorescence spectroscopy indicate that each fragment is mainly disordered. However, we find by a procedure of titration with trifluoroethanol that about 3% of fragment 1-22 is helical in water at 25 degrees C. Importantly, we have detected residual catalytic activity in fragment 23-110 toward GpUp and RNA and the ability to bind the polypeptide inhibitor of barnase, barstar, suggesting that this fragment can form a native-like conformation in water. The catalytic activity does not result from a small amount of contaminating impurity of parent enzyme or other ribonuclease, since the activity requires a 1:1 mole ratio of fragment to barstar for complete inhibition, and the activity is lost in much lower concentrations of urea than are required to denature the parent enzyme. There is a very weak signal in the near-UV CD spectrum of the large fragment.(ABSTRACT TRUNCATED AT 250 WORDS)
Kippen
A D
AD
Department of Chemistry, University of Cambridge, U.K.
Sancho
J
J
Fersht
A R
AR
D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins; D001665:Binding Sites; D002384:Catalysis; D002942:Circular Dichroism; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D012260:Ribonucleases / Q000737:chemistry* / Q000235:genetics; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D014270:Trifluoroethanol / Q000494:pharmacology; D014508:Urea
D001426:Bacterial Proteins; D010446:Peptide Fragments; D014270:Trifluoroethanol; D014508:Urea; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease