title: Evaluation of a dosing method for studying ergonovine effects in cattle.
abstract: We evaluated the feasibility of using miniosmotic pumps as a way to continuously treat cattle with a singular ergot alkaloid (ergonovine) of known content, thus mimicking the natural fescue toxicosis disease state, but allowing study of specific alkaloid effects. Dosing animals with increasing amounts of ergonovine via miniosomotic pumps, followed by daily acquisition of plasma samples for high-performance liquid chromatographic determination of the alkaloid, resulted in stepwise increases in plasma ergonovine concentration. However, despite the detectable blood concentration of ergonovine, calves did not have typical clinical signs of ergot alkaloid toxicosis. Similarly, serum prolactin concentration was unaffected by ergonovine in these cattle, implicating some other alkaloid of endophyte-infested fescue as causative of the usual prolactin-suppressive response. The results confirm use of this animal dosing method to study biological effects of singular purified alkaloids of known amount, without bioavailability concerns. Thus, this dosing method will facilitate studies to determine the harmful effects of individual alkaloids found in toxic tall fescue, and ultimately, to alleviate their costly effects in cattle, horses, and other species.
authors.affiliation: Department of Environmental Practice, University of Tennessee, Knoxville 37901-1071.
authors.forename: J W
authors.initials: JW
authors.lastname: Oliver
authors.forename: R D
authors.initials: RD
authors.lastname: Linnabary
authors.forename: L K
authors.initials: LK
authors.lastname: Abney
authors.forename: K R
authors.initials: KR
authors.lastname: van Manen
authors.forename: R
authors.initials: R
authors.lastname: Knoop
authors.forename: H S
authors.initials: HS
authors.lastname: Adair
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004874:Ergonovine / Q000008:administration & dosage* / Q000097:blood / Q000633:toxicity*; D006339:Heart Rate / Q000187:drug effects*; D007260:Infusion Pumps / Q000662:veterinary; D007262:Infusions, Intravenous / Q000662:veterinary*; D008297:Male; D009919:Orchiectomy; D010974:Platelet Aggregation / Q000187:drug effects; D011388:Prolactin / Q000097:blood*; D012119:Respiration / Q000187:drug effects*
chemical_list: D011388:Prolactin; D004874:Ergonovine