Fluoride prevents degranulation of the azurophilic and specific granules in electropermeabilized neutrophils.
The effect of AlF4- on the degranulation process in human neutrophils was investigated. In intact neutrophils, AlF4- induced degranulation, whereas in electropermeabilized neutrophils AlF4- did not stimulate degranulation. In electropermeabilized neutrophils, fluoride ions proved to be inhibitory for the degranulation induced by addition of Ca2+ and/or GTP-gamma-S. Another phosphatase inhibitor, okadaic acid, inhibited degranulation induced by Ca2+ or by GTP-gamma-S but not degranulation induced by the combination of Ca2+ plus GTP-gamma-S. It is concluded that under suboptimal conditions of stimulation with Ca2+ or GTP-gamma-S, protein dephosphorylation plays an important role in the degranulation response in human neutrophils.
Niessen
H W
HW
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Kessels
G C
GC
Verhoeven
A J
AJ
D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D015550:Cell Degranulation / Q000187:drug effects*; D004988:Ethers, Cyclic / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000502:physiology; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D019319:Okadaic Acid; D010539:Permeability; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D060149:Tetraspanin 30
D015703:Antigens, CD; D000951:Antigens, Neoplasm; C554586:CD63 protein, human; D015815:Cell Adhesion Molecules; D004988:Ethers, Cyclic; D008562:Membrane Glycoproteins; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D019319:Okadaic Acid; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D019204:GTP-Binding Proteins; D005459:Fluorides