Effect of luminal angiotensin II on proximal tubule fluid transport: role of apical phospholipase A2.
Recent micropuncture studies showed the existence of high concentrations of angiotensin II (ANG II) in proximal tubular fluid. In the present study, we have examined the effect of luminal ANG II, alone and in combination with peritubular ANG II, on fluid transport (JV) in the isolated perfused rabbit proximal convoluted tubule. In comparison with peritubular ANG II, luminal ANG II caused a similar but more potent biphasic effect on JV. At 10(-11) M, luminal ANG II maximally increased JV to 204 +/- 22% of the baseline compared with 142 +/- 10% by peritubular ANG II at 10(-10) M. At 10(-8) M, luminal ANG II suppressed JV to 9.7 +/- 16% of the baseline compared with 64 +/- 14% by peritubular ANG II. When luminal and peritubular ANG II were combined at concentrations that impose similar effect on JV, the effects of luminal and peritubular ANG II were not additive. However, when combined at concentrations that would otherwise impose opposing effects on JV, the stimulatory effect predominated. In support of the role of apical phospholipase A2 (PLA2) on the effect of luminal ANG II, ANG II stimulated PLA2 activity in isolated brush-border membrane vesicles, and addition of PLA2 inhibitor, mepacrine or dibucaine, to the luminal perfusate attenuated the effect of luminal ANG II on JV. In summary, these studies show a potent effect of luminal ANG II on proximal tubule JV involving activation of brush-border membrane PLA2. When combined, luminal and peritubular ANG II exert their effects in concert on proximal tubule JV.
Li
L
L
Division of Nephrology, Sepulveda Veterans Affairs Medical Center, California 91343.
Wang
Y P
YP
Capparelli
A W
AW
Jo
O D
OD
Yanagawa
N
N
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D003992:Dibucaine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D007700:Kinetics; D008297:Male; D010477:Perfusion; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054467:Phospholipases A2; D011796:Quinacrine / Q000494:pharmacology; D011817:Rabbits
D000804:Angiotensin II; D010741:Phospholipases A; D054467:Phospholipases A2; D011796:Quinacrine; D003992:Dibucaine