Prostaglandin E (dmPGE2) action in vitro on the activity of rat liver Golgi apparatus galactosyltransferase.
In vitro addition of 16,16'-dimethyl prostaglandin E2 to Golgi-rich membrane fraction in final concentration of 0.1 microgram/1 mg of protein increased generally the activity of galactosyltransferase in comparison with control. The percentage of phospholipids in the whole fraction was similar in both investigated groups, only the sum of phosphatidylethanolamine+phosphatidic acid was significantly lower after addition of dmPGE2 than in the control (0.001 < P < 0.01).
Kordowiak
A M
AM
Department of Animal Biochemistry, Jagiellonian University, Cracow, Poland.
Tomecki
J
J
Procyk
K
K
Kapusta
P
P
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology*; D000818:Animals; D005260:Female; D005700:Galactosyltransferases / Q000378:metabolism*; D006056:Golgi Apparatus / Q000201:enzymology / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D009237:N-Acetyllactosamine Synthase / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D010743:Phospholipids; D005700:Galactosyltransferases; D009237:N-Acetyllactosamine Synthase; D015064:16,16-Dimethylprostaglandin E2