title: Expression of functional protease and subviral particles by vaccinia virus containing equine infectious anaemia virus gag and 5' pol genes.
abstract: Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gag gene (VGag) or gag plus the 5' pol encoding protease (VGag/PR) were evaluated with monoclonal antibody to a p26 capsid protein linear epitope (QEISKFLTD). Both recombinant viruses expressed Gag precursor protein (55K) whereas only VGag/PR expressed a detectable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.
authors.affiliation: Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University.
authors.forename: T C
authors.initials: TC
authors.lastname: McGuire
authors.forename: K I
authors.initials: KI
authors.lastname: O'Rourke
authors.forename: T V
authors.initials: TV
authors.lastname: Baszler
authors.forename: S R
authors.initials: SR
authors.lastname: Leib
authors.forename: A L
authors.initials: AL
authors.lastname: Brassfield
authors.forename: W C
authors.initials: WC
authors.lastname: Davis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D015685:Fusion Proteins, gag-pol / Q000096:biosynthesis / Q000235:genetics*; D015750:Genes, gag / Q000235:genetics; D015751:Genes, pol / Q000235:genetics; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D007668:Kidney / Q000382:microbiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational / Q000235:genetics; D011533:Proviruses; D012367:RNA, Viral / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D014616:Vaccinia virus / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015685:Fusion Proteins, gag-pol; D012367:RNA, Viral; D011994:Recombinant Proteins; D010450:Endopeptidases