Characterization of a neuropathic pain model: sciatic cryoneurolysis in the rat.
Cryoanalgesia, the technique of freezing peripheral nerves, is used clinically for the treatment of postoperative and chronic pain. Paradoxically, this same technique produces characteristics in a rat model suggestive of neuropathic pain. We have developed a peripheral neuropathy model by freezing the proximal sciatic nerve (sciatic cryoneurolysis, SCN) using a cryoprobe cooled to -60 degrees C in a 30/5/30 sec freeze-thaw-freeze sequence. Each freeze cycle produced a transient ice ball on the surface of the nerve. These studies provide behavioral evidence that SCN is a valid mononeuropathy animal model. All animals demonstrate some degree of autotomy following SCN. The average onset of autotomy occurs 4 days postoperatively and peaks in severity and incidence at 14 days. By examining the latency of responses to a noxious heat stimulus, we have shown there is no direct relationship between an hypoesthetic paw and autotomy, i.e., autotomy did not occur immediately after the freeze lesion when the limb was dysfunctional. Rather, autotomy peaked when sensation was returning to the affected limb. The transient time course of certain behaviors including hypoesthesia and possible return of limb sensation, autotomy, touch-evoked allodynia, foot edema and the presence of spontaneous nociceptive behaviors demonstrate a multiple phase nociceptive process. The temporary nature of these nociceptive behaviors is in sharp contrast to the prolonged bilateral mechanical allodynia evident when these behaviors subside. The surgical anesthetics used during the SCN procedure are shown to variably alter or suppress autotomy following SCN.(ABSTRACT TRUNCATED AT 250 WORDS)
DeLeo
Joyce A
JA
Anesthesid Research Laboratory, Department of Anesthcsiology, Dartmouth-Hutchcok Medical Centre, Lebanon, NH 03756 USA Anesthesid Research Laboratory, Department of Pharmacology, Dartmouth-Hutchcok Medical Centre, Lebanon, NH 03756 USA Anesthesid Research Laboratory, Department of Physiology, Dartmouth-Hutchcok Medical Centre, Lebanon, NH 03756 USA.
Coombs
Dennis W
DW
Willenbring
Stan
S
Colburn
Raymond W
RW
Fromm
Christian
C
Wagner
Rochelle
R
Twitchcll
Bonnie B
BB
D000758:Anesthesia; D000818:Animals; D001522:Behavior, Animal; D002908:Chronic Disease; D017679:Cryotherapy; D003714:Denervation; D004195:Disease Models, Animal; D008297:Male; D009619:Nociceptors / Q000502:physiology; D010146:Pain / Q000503:physiopathology* / Q000523:psychology; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology*