The activation of factor VII in citrated plasma by charged long-chain saturated fatty acids at the interface of large triglyceride-rich lipoproteins.
The prolonged incubation of dilute plasma on ice in the presence of added sulphatide vesicles or the long-chain saturated fatty acids (FA) stearic acid (C18:0) or behenic acid (C22:0) induced a concentration-dependent increase in factor VII coagulant activity (VIIc). The addition of FA at various ratios to human serum albumin showed the micellar non-bound pool to be responsible for this effect, FA bound to the high-affinity or low-affinity binding sites of albumin having no influence on VIIc. Plasma VIIc also increased following addition of behenate-enriched lipoprotein particles produced by incubation of the d < 1.006 g/ml lipoprotein fraction with this FA, or addition of lipoprotein remnants produced by pre-incubation of the d < 1.006 g/ml fraction with lipoprotein lipase. Long-chain saturated fatty acids in the interface of lipoprotein remnants, produced by the interaction of triglyceride-rich lipoprotein particles with lipoprotein lipase, appear to provide a surface that activates the contact system of coagulation and subsequently factor VII.
Mitropoulos
K A
KA
MRC Epidemiology and Medical Care Unit, Wolfson Institute of Preventive Medicine, Medical College of St Bartholomew's Hospital, London, UK.
Reeves
B E
BE
Miller
G J
GJ
D000925:Anticoagulants; D002951:Citrates; D019343:Citric Acid; D005167:Factor VII / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008071:Lipoprotein Lipase / Q000494:pharmacology; D008074:Lipoproteins / Q000737:chemistry*; D008079:Lipoproteins, VLDL / Q000737:chemistry / Q000494:pharmacology; D008081:Liposomes; D010949:Plasma; D011247:Pregnancy; D012709:Serum Albumin; D013433:Sulfoglycosphingolipids; D014280:Triglycerides / Q000032:analysis*
D000925:Anticoagulants; D002951:Citrates; D005227:Fatty Acids; D008074:Lipoproteins; D008079:Lipoproteins, VLDL; D008081:Liposomes; D012709:Serum Albumin; D013433:Sulfoglycosphingolipids; D014280:Triglycerides; D019343:Citric Acid; D005167:Factor VII; D008071:Lipoprotein Lipase; C007547:behenic acid