title: Role of processing of the oligosaccharide chains in the affinity of lipoprotein lipase for heparin.
abstract: The role of processing of the oligosaccharide chains in the affinity of lipoprotein lipase (LPL) for heparin was examined in 3T3-L1 adipocytes. 43% of 35S-labeled LPL subunits in tunicamycin (TUN)-treated cells did not bind to a heparin-Sepharose column and 46% was eluted with 0.6 M NaCl. 11% of LPL subunits in castanospermine (CSTP)-treated cells did not bind to the column and 38% was eluted with 0.6 M NaCl. In contrast, as in untreated cells, LPL subunits in 1-deoxymannojirimycin (dMM)-treated and swainsonine (SW)-treated cells almost all bound to the column and over 93% of the subunits bound were eluted with 1.5 M NaCl. Thus, core glycosylation and subsequent removal of the distal glucose residue from oligosaccharide chains of LPL in the endoplasmic reticulum (ER) is required for acquisition of a higher affinity for heparin.
authors.affiliation: Department of Medical Biochemistry, School of Medicine, Ehime University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Masuno
authors.forename: H
authors.initials: H
authors.lastname: Okuda
mesh_terms: D017485:1-Deoxynojirimycin / Q000494:pharmacology; D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006031:Glycosylation; D006493:Heparin / Q000378:metabolism*; D007212:Indolizines / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009844:Oligosaccharides / Q000378:metabolism*; D013379:Substrate Specificity; D017026:Swainsonine / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology; D054836:Indolizidines
chemical_list: D017485:1-Deoxynojirimycin; D006493:Heparin; D007212:Indolizines; D008071:Lipoprotein Lipase; D009844:Oligosaccharides; D017026:Swainsonine; D014415:Tunicamycin; C037806:castanospermine; D054836:Indolizidines