Effects of osteogenesis on dermal fibroblasts cultured in vitro.
Fibroblast were harvested from in vitro culture of split-thickness skin of New Zealand rabbit. Some opaque bone nodules were found in the culture flasks when the fibroblasts underwent subculture for 8 days. After a 37-day subculture of fibroblasts, some of the nodules might enlarge extend and form trabecular-like or flake-like structures. The above-mentioned structures revealed golden-yellow fluorescence under the blueviolet light fluorescence microscope when the specimens were labeled with tetracycline intravitally, denoting that they were newly formed bone tissues. Both calcium salt and collagen stainings for these structures showed positive reaction, fully reflecting the calcium and collagen composition. Osteogenesis caused by dermal fibroblast cultured in vitro was thus verified.
Xu
R H
RH
Basic Research Section, Shanghai Institute of Traumatology and Orthopedics, Shanghai Second Medical University.
Rao
H M
HM
Zhu
Y P
YP
Chai
B F
BF
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D005347:Fibroblasts / Q000502:physiology; D017102:Fracture Healing; D008856:Microscopy, Fluorescence; D010012:Osteogenesis; D011817:Rabbits; D012867:Skin / Q000166:cytology*
D003094:Collagen