title: Determination of aflatoxins in food by use of an automatic work station.
abstract: An automated HPLC method with postcolumn derivatization is described for the determination of aflatoxins B1, B2, G1 and G2 after an immunoaffinity column clean-up. It can be used for the determination of aflatoxins in a variety of foodstuffs such as nuts, nut-like products (pistachios, almonds, etc.) and dried fruit. The aflatoxins are extracted with methanol-water, followed by a filtration step. Dilution of the extract, mixing, immunoaffinity column clean-up, elution of the aflatoxins and optional on-line HPLC are performed by an automatic work station (Zymark BenchMate). The subsequent HPLC analysis includes a postcolumn derivatization step with iodine solution and fluorimetric detection. The method compared well with manual techniques and another automated method.
authors.affiliation: Dr. Wiertz-Dipl.-Chem. Eggert-Dr. Jörissen GmbH, Handels- und Umweltschutzlaboratorium, Hamburg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Niedwetzki
authors.forename: G
authors.initials: G
authors.lastname: Lach
authors.forename: K
authors.initials: K
authors.lastname: Geschwill
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D001331:Automation; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*
chemical_list: D000348:Aflatoxins