Myosin light chain kinase expression during smooth muscle development.
The expression of smooth muscle myosin light chain kinase (MLCK) was investigated during chicken gizzard development. The molecular weight and the antigenic properties of MLCK did not change during development. The use of anion exchange high performance liquid chromatography (HPLC) enabled us to distinguish between MLCKs from post-hatched and adult chickens. A partial amino acid sequence determination of 4-day-old gizzard MLCK failed to disclose differences in the primary sequences of the two proteins. The results suggest that MLCK has the same primary sequence in all stages of gizzard development, although charge variants due to post-translational modifications may exist.
Dalla Libera
L
L
Department Biomedical Sciences, University of Padova, Italy.
Collins
J H
JH
D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid; D004790:Enzyme Induction; D005895:Gizzard, Avian / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D024510:Muscle Development; D009130:Muscle, Smooth / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D009219:Myosin-Light-Chain Kinase / Q000096:biosynthesis* / Q000235:genetics; D011499:Protein Processing, Post-Translational
D007527:Isoenzymes; D009219:Myosin-Light-Chain Kinase