title: Genetic structures associated with spread of the type Ia trimethoprim-resistant dihydrofolate reductase gene amongst Escherichia coli strains isolated in the Nottingham area of the United Kingdom.
abstract: DNA probes for specific integrase genes were used to study 122 R plasmids encoding the predominant trimethoprim-insusceptible type Ia dihydrofolate reductase (DHFR) found in clinical isolates of Escherichia coli. The predominance of the type Ia DHFR was thought to result from the location of its gene on transposon Tn7, but of trimethoprim R plasmids carrying this gene that were collected between 1978 and 1983, between 1987 and 1988, and during 1992, only 49/60 (81.6%), 30/43 (69.8%) and 9/19 (47.4%) respectively hybridized with a probe for the Tn7 integrase gene. It has been suggested that novel genetic elements termed 'integrons' may play an important role in the dissemination of antibiotic resistance genes. Known integrons encode an integrase similar to that encoded by transposon Tn21, and 28 Tn7-negative plasmids (10/60 from 1978-83, 10/43 from 1987-8 and 8/19 from 1992) showed homology with a probe specific for the Tn21 integrase gene. Six plasmids were negative with both probes. It is concluded that Tn7 has played an important role in the dissemination of the gene encoding the type Ia DHFR amongst clinical isolates of E. coli in the Nottingham region of the UK, but that other genetic structures, some of which seem to have an integrase function similar to that of known integrons, may be playing an increasingly significant role.
authors.affiliation: Department of Microbiology, University Hospital, Queen's Medical Centre, Nottingham.
authors.forename: K J
authors.initials: KJ
authors.lastname: Towner
authors.forename: A
authors.initials: A
authors.lastname: Brennan
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: C A
authors.initials: CA
authors.lastname: Holtham
authors.forename: J L
authors.initials: JL
authors.lastname: Brough
authors.forename: G I
authors.initials: GI
authors.lastname: Carter
mesh_terms: D001437:Bacteriuria / Q000382:microbiology; D015342:DNA Probes; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D017403:In Situ Hybridization; D011815:R Factors / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014296:Trimethoprim Resistance / Q000235:genetics*; D006113:United Kingdom
chemical_list: D015342:DNA Probes; D013762:Tetrahydrofolate Dehydrogenase