Evolution of translational elongation factor (EF) sequences: reliability of global phylogenies inferred from EF-1 alpha(Tu) and EF-2(G) proteins.
The EF-2 coding genes of the Archaea Pyrococcus woesei and Desulfurococcus mobilis were cloned and sequenced. Global phylogenies were inferred by alternative tree-making methods from available EF-2(G) sequence data and contrasted with phylogenies constructed from the more conserved but shorter EF-1 alpha(Tu) sequences. Both the monophyly (sensu Hennig) of Archaea and their subdivision into the kingdoms Crenarchaeota and Euryarchaeota are consistently inferred by analysis of EF-2(G) sequences, usually at a high bootstrap confidence level. In contrast, EF-1 alpha(Tu) phylogenies tend to be inconsistent with one another and show low bootstrap confidence levels. While evolutionary distance and DNA maximum parsimony analyses of EF-1 alpha(Tu) sequences do show archaeal monophyly, protein parsimony and DNA maximum-likelihood analyses of these data do not. In no case, however, do any of the tree topologies inferred from EF-1 alpha(Tu) sequence analyses receive significant bootstrap support.
Creti
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R
Istituto Pasteur-Fondazione Cenci-Bolognetti, Dipt. Biopatologia Umana, Università di Roma I, Policlinico Umberto I., Italy.
Ceccarelli
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E
Bocchetta
M
M
Sanangelantoni
A M
AM
Tiboni
O
O
Palm
P
P
Cammarano
P
P
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D016013:Likelihood Functions; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D020648:Peptide Elongation Factor 1; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors