Staphylococcin 1580 is identical to the lantibiotic epidermin: implications for the nature of bacteriocins from gram-positive bacteria.
Staphylococcin 1580 was purified to homogeneity from culture supernatants of Staphylococcus epidermidis 1580 by means of adsorption to XAD 2, cation exchange chromatography, and high-performance liquid chromatography on reversed-phase C18. The purified active substance migrated in sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an apparent M(r) of approximately 2,000. Amino acid analysis, mass determination (2,165 Da) and N-terminal sequencing (Ile-Ala-Xaa-Lys-Phe-Ile-Xaa-Xaa-Pro-Gly-Xaa-Ala-Lys-block) demonstrated that staphylococcin 1580 is identical to epidermin, a lanthionine-containing antibiotic peptide (lantibiotic).
Sahl
H G
HG
Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, Federal Republic of Germany.
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001430:Bacteriocins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010455:Peptides; C017724:staphylococcin; C050558:epidermin