title: TATA-binding protein-independent initiation: YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNA.
abstract: YY1 is a zinc finger transcription factor whose DNA-binding motif exhibits the properties of an initiator element. Only three factors were required to direct specific basal transcription on a supercoiled template DNA carrying the YY1 initiator: YY1, general transcription factor IIB, and RNA polymerase II. This minimal in vitro reaction did not require the TATA-binding protein (TBP). We propose that, under appropriate conditions, YY1 can function like TBP, as a factor that binds to the core promoter and recruits the polymerase to the initiation complex.
authors.affiliation: Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, New Jersey 08544-1014.
authors.forename: A
authors.initials: A
authors.lastname: Usheva
authors.forename: T
authors.initials: T
authors.lastname: Shenk
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D001483:Base Sequence; D004278:DNA, Superhelical / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology*; D000229:Dependovirus / Q000235:genetics; D050981:Erythroid-Specific DNA-Binding Factors; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000502:physiology; D012319:RNA Polymerase II / Q000502:physiology*; D035181:TATA-Box Binding Protein; D013698:Templates, Genetic; D035581:Transcription Factor TFIIB; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*; D050986:YY1 Transcription Factor
chemical_list: D004278:DNA, Superhelical; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494698:YY1 protein, human; D012319:RNA Polymerase II