Automated latex agglutination immunoassay of serum ferritin with a centrifugal analyzer.
A considerable demand for convenient, rapid, inexpensive assays of ferritin in serum has been generated in recent years in hospital laboratories and blood banks. We describe a simple and rapid particle-enhanced turbidimetric immunoassay suitable for routine application in a Monarch 2000 centrifugal analyzer with commercially available reagents. This fully automated assay (y) requires no pretreatment of sample, and correlation with a two-step sandwich ELISA (x) is excellent (y = 1.018x + 0.397, Sy/x = 0.027). The analytical range extends from 5 to 900 micrograms/L. Intraassay imprecision (CV) ranged from 1.1% to 5% for various specimen concentrations. Interassay imprecision ranged from 2.2% for above-normal concentrations (755 micrograms/L) to 9.5% for low concentrations (39 micrograms/L). No specimen-related carryover was detected. The method has been useful in our predeposit autologous blood transfusion program for rapid assessment of iron status in patients undergoing repeated phlebotomies.
Simó
J M
JM
Centre de Recerca Biomèdica, Hospital de Sant Joan de Reus, Spain.
Joven
J
J
Clivillé
X
X
Sans
T
T
D000328:Adult; D001322:Autoanalysis; D001804:Blood Transfusion, Autologous; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005293:Ferritins / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000706:statistics & numerical data; D007841:Latex Fixation Tests; D008297:Male; D008875:Middle Aged; D011786:Quality Control; D012016:Reference Values; D012680:Sensitivity and Specificity
D005293:Ferritins