Expression of recoverin mRNA in the human retina: localization by in situ hybridization.
The localization of mRNA encoding recoverin in the human retina was examined by in situ hybridization. The riboprobe utilized in this study was transcribed from a cDNA clone containing the complete coding region of human recoverin. The riboprobe hybridized extensively with the photoreceptor inner segments, and the outer nuclear layer. Specific hybridization was also seen in a subpopulation of cells in the inner nuclear layer, and occasionally in cells of the ganglion cell layer (GCL). Recoverin-like immunoreactivity was observed in all photoreceptors, and in a subpopulation of bipolar cells. Very robust immunolabeling was observed in a small population of cells in the GCL. This study suggests that recoverin, or a recoverin-like protein, is produced by at least three different cell types in the human retina: photoreceptors, bipolars and rare cells in the ganglion cell layer.
Wiechmann
A F
AF
Department of Neurobiology and Anatomy, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC.
Hammarback
J A
JA
D000328:Adult; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D005136:Eye Proteins; D015870:Gene Expression; D051597:Hippocalcin; D006801:Humans; D017403:In Situ Hybridization; D007907:Lens Nucleus, Crystalline / Q000737:chemistry; D008074:Lipoproteins; D008297:Male; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger / Q000032:analysis*; D051603:Recoverin; D012160:Retina / Q000737:chemistry*; D017948:Retinal Rod Photoreceptor Cells / Q000737:chemistry
D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; C496731:RCVRN protein, human; D012333:RNA, Messenger; D051603:Recoverin; D051597:Hippocalcin