Effects of differential rearing on the structure of perforated synapses in the granule cell layer of the rat's accessory olfactory bulb.
The effects of differential rearing on synaptic morphology in the granule cell layer of the accessory olfactory bulb (AOB) were examined in adult rats. Forty-day-old male rats were housed in one of three ways: individually (isolated condition, IC); with four males per cage (unisexual condition, UC); or with two males and two females per cage (social condition, SC). After 2 months, the animals were killed for electron microscopy. Two types of synapses were classified: (1) perforated synapses, which are characterized by discontinuities in their postsynaptic thickenings, and (2) non-perforated synapses. The length of the synaptic contact zone as well as the area and the length of the perimeter of both the pre- and postsynaptic terminals were measured in each observed synapse. For perforated synapses, the length of the synaptic contact zone was significantly greater in the UC and the SC than in the IC. For the presynaptic terminals of perforated synapses, the area was greater in the UC and the SC than in the IC, and the length of the perimeter was greater in the UC than in the IC. In contrast, there was no difference in the area and the length of the perimeter among the three groups for postsynaptic terminals. For non-perforated synapses, there was no statistically significant difference in either the area or the length of the perimeter of pre- and postsynaptic terminals among the three experimental groups, although the length of the synaptic contact zone was greater in the SC than in the IC.(ABSTRACT TRUNCATED AT 250 WORDS)
Ichikawa
M
M
Department of Anatomy and Embryology, Tokyo Metropolitan Institute for Neuroscience, Japan.
Matsuoka
M
M
Mori
Y
Y
D000704:Analysis of Variance; D000822:Animal Husbandry; D000818:Animals; D008297:Male; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000648:ultrastructure; D009473:Neuronal Plasticity; D009830:Olfactory Bulb / Q000166:cytology / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012931:Social Environment; D013569:Synapses / Q000648:ultrastructure*