Identification of conserved domains in the delta 12 desaturases of cyanobacteria.
Cyanobacterial genes for enzymes that desaturate fatty acids at the delta 12 position, designated desA, were isolated from Synechocystis PCC6714, Synechococcus PCC7002 and Anabaena variabilis by cross-hybridization with a DNA probe derived from the desA gene of Synechocystis PCC6803. The genes of Synechocystis PCC6714, Synechococcus PCC7002 and A. variabilis encode proteins of 349, 347 and 350 amino acid residues, respectively. The transformation of Synechococcus PCC7942 with the desA genes from Synechocystis PCC6714, Synechococcus PCC7002 and A. variabilis was associated with the ability to introduce a second double bond at the delta 12 position of fatty acids. The amino acid sequence of the products of the desA genes revealed the presence of four conserved domains. Since one of the conserved domains was also found in the amino acid sequences of omega 3 desaturases of Brassica napus and mung bean, this domain may play an essential role in the introduction of a double bond into fatty acids bound to membrane lipids.
Sakamoto
T
T
Department of Molecular Biomechanics, Graduate University of Advanced Studies, Okazaki, Japan.
Wada
H
H
Nishida
I
I
Ohmori
M
M
Murata
N
N
D000595:Amino Acid Sequence; D017033:Anabaena / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial / Q000302:isolation & purification; D044943:Fatty Acid Desaturases / Q000235:genetics*; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D005227:Fatty Acids; D044943:Fatty Acid Desaturases; C040448:delta-12 fatty acid desaturase