Cloning, sequencing and expression in E. coli of interferon-omega 1 gene.
Human interferon omega 1 (huIFN-omega 1) gene was isolated and cloned from chromosome DNA derived from a Chinese fetal liver via polymerase chain reaction (PCR). By determining its nucleotide sequence we proved that the 88th codon should be GGA, coding for Gly. After engineering the original IFN-omega 1 gene clone to a form that may be expressed as a nonfused protein, we also took the IFN-omega 1 gene under the control of the PRPL promoter with an expression vector pBV220 in E. coli. The antivirus activity of the recombinant IFN-omega 1 is about 6.5 x 10(7) units/L CULTURE (OD600 = 0.75). Since IFN-omega 1 not only has antivirus activity but also shows considerably high homology with animal trophoblast proteins which have been proved antiluteolysins as a maternal recognition signal for pregnancy, we believe that study on it will be practically and theoretically significant.
Li
M F
MF
Institute of Virology, Chinese Academy of Preventive Medicine, National Laboratory of Molecular Virology, Beijing, PRC.
Zeng
Q
Q
Zhou
Y
Y
Guo
H Y
HY
Hou
Y D
YD
D000595:Amino Acid Sequence; D001483:Base Sequence; D002875:Chromosomes; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005333:Fetus; D015870:Gene Expression; D005818:Genetic Engineering; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics*; D008099:Liver; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D007370:Interferon Type I; C063743:interferon omega 1; D004247:DNA