title: Studies on red cell glucose-6-phosphate dehydrogenase: evaluation of reference values.
abstract: The analytical, intra-individual, inter-individual variation and reference values were determined for red cell glucose-6-phosphate dehydrogenase (G6PD). Different procedures for the conditions for storage of red blood cells and the preparation of haemolysates were investigated. A total of 2170 samples of blood were taken from apparently healthy persons-1212 males and 958 females--from randomly selected villages and city centres in the southern part of Turkey. Analytical variation, intra-individual variation and inter-individual variation were 8.67%, 32.8% and 31.8%, respectively. The mean (SD) for G6PD was 8.6 (3.3) IU/gHb. The index of individuality, 1.03, showed that the reference intervals could be used for diagnostic purposes. Whole blood or a red cell pellet could be stored in physiological saline for one week at 4 degrees C or -20 degrees with little loss of activity. Two of three different procedures for the preparation of haemolysate gave data that showed no statistical difference and were equally satisfactory.
authors.affiliation: Biochemistry Department, Cukurova University Medical School, Adana, Turkey.
authors.forename: G T
authors.initials: GT
authors.lastname: Yüregir
authors.forename: K
authors.initials: K
authors.lastname: Aksoy
authors.forename: A
authors.initials: A
authors.lastname: Arpaci
authors.forename: I
authors.initials: I
authors.lastname: Unlükurt
authors.forename: A
authors.initials: A
authors.lastname: Tuli
mesh_terms: D001800:Blood Specimen Collection; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D006801:Humans; D008297:Male; D012016:Reference Values
chemical_list: D005954:Glucosephosphate Dehydrogenase