Further evidence for an active site polypeptide of galactosyl transferase.
In a previous report it was shown that galactosyl transferase activity after blotting from acrylamide gel was present in a molecular weight range of less than 14 kDa, in Triton X-100 (1). Molecular sieve chromatography on Superose 12, in the presence of Triton X-100, gave the same result. The low molecular weight activity peak was eluted together with peptides as a part of the covalent structure of the enzyme or as absolutely requires effectors. Peptide mapping showed a new poly-lysine-like peptide and a new hydrophobic peptide in this low molecular weight activity peak as effectors of the enzyme inside its hydrophobic environment.
Plancke
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Laboratoire de Chimie Biologique, Université des Sciences et Techniques de Lille Flandre-Artois, Unité Mixte de Recherche du CNRS No. 111, Villeneuve d'Ascq, France.
D000494:Allosteric Regulation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D004588:Electrophoresis, Cellulose Acetate; D006056:Golgi Apparatus / Q000201:enzymology; D007768:Lactalbumin / Q000737:chemistry; D007788:Lactose Synthase / Q000737:chemistry*; D008647:Mesocricetus; D008970:Molecular Weight; D009237:N-Acetyllactosamine Synthase / Q000737:chemistry; D017830:Octoxynol; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D010449:Peptide Mapping; D017474:Receptors, Laminin / Q000737:chemistry
D000596:Amino Acids; D010446:Peptide Fragments; D017474:Receptors, Laminin; D017830:Octoxynol; D007768:Lactalbumin; D007788:Lactose Synthase; D009237:N-Acetyllactosamine Synthase