Two isoforms of G protein-coupled receptor kinase 4 identified by molecular cloning.
Using PCR we found that G protein-coupled receptor kinase4 (GRK4) mRNA is expressed only in brain out of several tissues tested. In the brain two amplification products were generated. Sequence analysis revealed that the two fragments differed only by the presence or absence of an in-frame-sequence of 96 bp/32 amino acids, located near the N-terminal of the kinase. This demonstrates the existence of two isoforms of GRK4 which were named GRK4A and GRK4B in the presence or absence of the insert, respectively. This is the first evidence that, within the GRKs gene family, different isoforms do exist.
Sallese
M
M
Istituto di Ricerche Farmacologiche Mario Negri, Santa Maria Imbaro, Italy.
Lombardi
M S
MS
De Blasi
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D054771:G-Protein-Coupled Receptor Kinase 4; D019204:GTP-Binding Proteins / Q000096:biosynthesis*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009504:Neutrophils / Q000378:metabolism*; D010766:Phosphorylation; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D012333:RNA, Messenger; D020794:Receptor Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D054771:G-Protein-Coupled Receptor Kinase 4; C517855:GRK4 protein, human; D019204:GTP-Binding Proteins