title: Expression of human sialophorin (CD43) in a human x mouse somatic cell hybrid.
abstract: We have studied the expression of the human sialophorin (CD43) molecule in a human x mouse somatic cell hybrid (H-8). The CD43 molecule was detected on the cell surface by immunofluorescence analysis and the hybrid cell possessed human chromosome 16 which carries the gene for CD43. Biochemical analysis showed that the cell line expressed a low molecular form of CD43, which was due to altered glycosylation. The cells reacted with anti-CD43 antibodies but behaved differently in response to the antibodies in the aggregation process. This indicates the crucial importance of the sugar moieties and epitope position in aggregation and subsequent activation mediated by CD43.
authors.affiliation: Institute of Genetics, Hungarian Academy of Sciences, Szeged.
authors.forename: H A
authors.initials: HA
authors.lastname: Ramadan
authors.forename: E
authors.initials: E
authors.lastname: Monostori
authors.forename: I
authors.initials: I
authors.lastname: Cserpan
authors.forename: I
authors.initials: I
authors.lastname: Ocsovszky
authors.forename: I
authors.initials: I
authors.lastname: Andó
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis* / Q000235:genetics; D002460:Cell Line; D002885:Chromosomes, Human, Pair 16; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D007621:Karyotyping; D051917:Leukosialin; D051379:Mice; D012795:Sialoglycoproteins / Q000032:analysis* / Q000235:genetics
chemical_list: D015703:Antigens, CD; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins; C497863:Spn protein, mouse