title: Agonist-specific coupling of a cloned Drosophila octopamine/tyramine receptor to multiple second messenger systems.
abstract: A cloned seven transmembrane-spanning Drosophila octopamine/tyramine receptor, permanently expressed in a Chinese hamster ovary cell line, both inhibits adenylate cyclase activity and leads to the elevation of intracellular Ca2+ levels by separate G-protein-coupled pathways. Agonists of this receptor (octopamine and tyramine), differing by only a single hydroxyl group in their side chain, may be capable of differentially coupling it to different second messenger systems. Thus, a single receptor may have a different pharmacological profile depending on which second messenger system is used to assay its efficacy.
authors.affiliation: AFRC Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, UK.
authors.forename: S
authors.initials: S
authors.lastname: Robb
authors.forename: T R
authors.initials: TR
authors.lastname: Cheek
authors.forename: F L
authors.initials: FL
authors.lastname: Hannan
authors.forename: L M
authors.initials: LM
authors.lastname: Hall
authors.forename: J M
authors.initials: JM
authors.lastname: Midgley
authors.forename: P D
authors.initials: PD
authors.lastname: Evans
mesh_terms: D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D004330:Drosophila / Q000378:metabolism*; D009655:Octopamine / Q000378:metabolism*; D011869:Radioligand Assay; D017982:Receptors, Biogenic Amine / Q000235:genetics / Q000378:metabolism*; D015290:Second Messenger Systems; D014439:Tyramine / Q000378:metabolism*
chemical_list: D017982:Receptors, Biogenic Amine; C027604:norsynephrine receptor; C066175:tyramine receptor; D009655:Octopamine; D000242:Cyclic AMP; D002118:Calcium; D014439:Tyramine