title: Proton NMR lipid profile of Leishmania donovani promastigotes.
abstract: The proton nuclear magnetic resonance (NMR) lipid profile of Leishmania donovani was obtained in the one-dimensional and two-dimensional modes. Partial assignments of lipid classes and individual lipids were obtained purely from the proton NMR spectrum of the mixture. A more complete assignment and quantitative analysis was achieved by prior separation of the lipids by high pressure liquid chromatography (HPLC) followed by proton NMR analysis of the fractions. This work showed that proton NMR spectroscopy could facilitate lipid analysis and classification of various parasitic protozoa and serve as a basis for rapid studies of comparative lipid metabolism in parasites.
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, London, UK.
authors.forename: R K
authors.initials: RK
authors.lastname: Adosraku
authors.forename: M M
authors.initials: MM
authors.lastname: Anderson
authors.forename: G J
authors.initials: GJ
authors.lastname: Anderson
authors.forename: G
authors.initials: G
authors.lastname: Choi
authors.forename: S L
authors.initials: SL
authors.lastname: Croft
authors.forename: V
authors.initials: V
authors.lastname: Yardley
authors.forename: J D
authors.initials: JD
authors.lastname: Phillipson
authors.forename: W A
authors.initials: WA
authors.lastname: Gibbons
mesh_terms: D000818:Animals; D002794:Choline / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004983:Ethanolamines / Q000032:analysis; D007893:Leishmania donovani / Q000737:chemistry* / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis* / Q000145:classification; D009682:Magnetic Resonance Spectroscopy; D010716:Phosphatidylinositols / Q000032:analysis; D011522:Protons
chemical_list: D004983:Ethanolamines; D008055:Lipids; D010716:Phosphatidylinositols; D011522:Protons; D002794:Choline