Superinduction of mitogen-stimulated interferon-gamma production and other lymphokines by Sendai virus.
We observed that Sendai virus preinduction of peripheral blood mononuclear cells and subsequent mitogenic stimulation resulted in: (i) Superproduction of interferon-gamma, (IFN-gamma) (ii) an increase in interleukin-2 (IL-2) synthesis that correlates with DNA synthesis when stimulated with phytohemagglutinin (PHA) or pokeweed mitogen (PWM) after treatment with the Sendai virus, while stimulation with Protein A from Staphylococcus aureus was not affected, and (iii) enhanced tumor necrosis factor-alpha (TNF-alpha) production in response to bacterial lipopolysaccharide (LPS). Treatment of monocyte cultures with LPS and cycloheximide or actinomycin-D inhibited the superinduction phenomenon. When cycloheximide was added at the viral induction time, the inhibition of TNF-alpha superproduction and DNA synthesis was still observed. These results suggest that Sendai virus lymphocyte superinduction is specific for a particular stimulatory pathway, not dependent on mRNA accumulation, and probably mediated by induction of an activating protein.
Costas
M A
MA
Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Mella
D
D
Criscuolo
M
M
Díaz
A
A
Finkielman
S
S
Nahmod
V E
VE
Arzt
E
E
D016516:CD4-CD8 Ratio; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis; D008222:Lymphokines / Q000096:biosynthesis*; D008934:Mitogens / Q000494:pharmacology*; D010222:Parainfluenza Virus 1, Human / Q000502:physiology*; D014176:Protein Biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis
D007369:Interferon Inducers; D007376:Interleukin-2; D008222:Lymphokines; D008934:Mitogens; D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins; D003609:Dactinomycin; D007371:Interferon-gamma; D004247:DNA; D003513:Cycloheximide