Immunocytochemical localization of androgen receptors in the scalp of the stumptail macaque monkey, a model of androgenetic alopecia.
The purpose of this study was to identify the distribution of androgen receptors in the bald and hairy scalp of adult male and female stumptail macaque monkeys by light microscopic biotin-avidin immunocytochemistry with a highly purified rat monoclonal antibody against the cloned human androgen receptor. Consistent, intense nuclear and minimal cytoplasmic immunostaining was observed in several distinct cell populations of the pilosebaceous unit including the dermal papilla, hair epithelium, outer root sheath, dermal sheath, and sebaceous gland. A similar distribution of androgen receptors was found in miniaturized and terminal anagen and telogen follicles of the bald and hairy scalp, respectively. Binding of androgen receptor antibody was also detected in dermal fibroblasts, basal and intermediate layers of the interfollicular epidermis, and duct and glandular cells of eccrine sweat glands. This investigation demonstrates the presence of androgen receptors in the pilosebaceous unit of the scalp of the stumptail macaque and also shows that their distribution is comparable to that previously reported for humans.
Diani
A R
AR
Upjohn Laboratories, Upjohn Company, Kalamazoo MI 49001.
Mills
C J
CJ
D000505:Alopecia / Q000473:pathology*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D008251:Macaca; D008297:Male; D011944:Receptors, Androgen / Q000032:analysis*; D012535:Scalp / Q000648:ultrastructure*; D012867:Skin / Q000648:ultrastructure*
D011944:Receptors, Androgen