title: Cyclin B1 expression in HeLa S3 cells studied by flow cytometry.
abstract: Using a procedure to stain cells simultaneously for cyclin B1 protein and DNA, we have examined cyclin B1 expression by flow cytometry in human cells under a variety of perturbing and nonperturbing conditions. The method described is useful for measuring relative differences in cyclin B level (immunochemically detectable epitope) as a function of cell cycle position on an individual cell basis and thus to examine cell cycle-related changes in cyclin B expression without prior cell synchronization. We show that in HeLaS3 cells, cyclin B1 accumulates in cells only after they become 4C and have resided in G2 for a short period of time. During colcemid-induced mitotic arrest cyclin B1 continues to accumulate in HeLa S3 cells, and under specific conditions of aphidicolin-induced unbalanced cell growth induced, cyclin B accumulates to supranormal levels prior to mitosis. Flow cytometric analysis of cyclin B expression and DNA content permits detailed examination of the effects of cell cycle perturbations on cyclin B expression under a variety of conditions.
authors.affiliation: Department of Biological Sciences, Stanford University, California 94305.
authors.forename: S W
authors.initials: SW
authors.lastname: Sherwood
authors.forename: D F
authors.initials: DF
authors.lastname: Rush
authors.forename: A L
authors.initials: AL
authors.lastname: Kung
authors.forename: R T
authors.initials: RT
authors.lastname: Schimke
mesh_terms: D016590:Aphidicolin / Q000494:pharmacology; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D003703:Demecolcine / Q000494:pharmacology; D005434:Flow Cytometry; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans
chemical_list: D016213:Cyclins; D016590:Aphidicolin; D004247:DNA; D003703:Demecolcine