[The characteristics of unscheduled DNA synthesis and of the changes in the structural parameters of human lymphocyte nuclei after the action of x-ray radiation in low doses and in combination with UV irradiation].
Effects of X-ray low doses (0.5-25 cGy) with following UV light (254 nm, 20 J/m2) on human peripheral blood lymphocytes were studied. Reparation response registered by unscheduled DNA synthesis (UDS) activity was demonstrated to be the most intensive after action of X-rays in dose ranges 2-3 cGy and 15-20 cGy, and least intensive after 10 cGy. In those cells where UV light was followed by X-rays, dose ranges 2-3 cGy and 15-20 cGy cause essential decrease of UDS as compared with UV-light action only. The most intensive UDS was in those UV-irradiated lymphocytes which where previously exposed to 10 cGy of X-rays. At the same time 10 cGy cause minimal reparation response without UV light. Possible mechanisms of discovered phenomena are discussed. In particular, reaction on 2-3 cGy might be a reorganization of genome for adaptive response or an evolution reserve cells response. From 10 cGy the reason of reaction might be a reparation, induced by radiation.
Spitkovskiĭ
D M
DM
Ermakov
A V
AV
Gorin
A I
AI
Pospekhova
N I
NI
Sorokinna
T A
TA
Talyzina
T A
TA
D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure*; D002478:Cells, Cultured / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis* / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays
D014316:Tritium; D004247:DNA; D013936:Thymidine
Osobennosti vneplanovogo sinteza DNK i izmeneniĭ strukturnykh parametrov iader limfotsitov cheloveka posle deĭstviia rentgenovskogo izlucheniia v malykh dozakh i v sochetanii s UF-oblucheniem.