title: Homotypic adhesion of rat B cells, but not T cells, in response to cross-linking of CD48.
abstract: Rat lymphocytes were found to aggregate in response to monoclonal antibodies to the glycosyl phosphatidylinositol (GPI)-anchored surface antigen CD48. This clustering required bivalent antibodies but was not Fc mediated. It was blocked by inhibitors of cellular metabolism and cytoskeletal function but not by antibodies to leucocyte function-associated antigen-1 (LFA-1) or intracellular adhesion molecule-1 (ICAM-1). The clusters were found to be due to homotypic adhesion of B cells, with T cells showing no response despite expressing equal levels of CD48. In addition, thymocytes, which are known to cluster in response to cross-linking of Thy-1, another GPI-anchored molecule, were found not to respond to cross-linking of CD48. These results suggest that specific signalling through CD48 in B cells, but not T cells, and through Thy-1, but not CD48, in thymocytes, lead to cell adhesion events. This differential signalling is interesting as neither CD48 nor Thy-1 have transmembrane or intracellular domains. Levels of CD48-associated protein kinase activity were very low in both B and T cells, and no difference in the susceptibility to cleavage with phosphatidylinositol-specific phospholipase C was detected between B- and T-cell CD48.
authors.affiliation: MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford.
authors.forename: D
authors.initials: D
authors.lastname: Garnett
authors.forename: A F
authors.initials: AF
authors.lastname: Williams
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000187:drug effects / Q000276:immunology*; D001402:B-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D000071178:CD48 Antigen; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D010770:Phosphotransferases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000071178:CD48 Antigen; C000604067:Cd48 protein, rat; D007141:Immunoglobulin Fc Fragments; D010770:Phosphotransferases; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase