Multi-target PCR analysis by capillary electrophoresis and laser-induced fluorescence.
Quantitative analysis of polymerase chain reaction (PCR) amplified HIV-1 DNA or cDNA fragments is attained using an automated system that combines capillary-gel electrophoresis (CGE) for high-efficiency separation and laser-induced fluorescence (LIF) for high-sensitivity detection. This system enables the detection of PCR-amplified multiple target DNA or cDNA in the same tube by a single injection with high precision.
Lu
W
W
Laboratory of Tumor Immunology, Hopital Laennec, Université de Paris V, France.
Han
D S
DS
Yuan
J
J
Andrieu
J M
JM
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D001331:Automation; D001483:Base Sequence; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods*; D005453:Fluorescence; D016655:HIV Core Protein p24 / Q000235:genetics; D015700:HIV Envelope Protein gp41 / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007834:Lasers; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis; D012680:Sensitivity and Specificity; D014771:Virion / Q000302:isolation & purification
D018076:DNA, Complementary; D004279:DNA, Viral; D016655:HIV Core Protein p24; D015700:HIV Envelope Protein gp41; D012367:RNA, Viral