Prevalence in Sweden of human papillomavirus type 16 infection in CIN II-III cases assessed by a PCR method.
The presence of human papillomavirus (HPV) 16 in cervical intraepithelial neoplasia (CIN)II-III cases was studied by means of a polymerase chain reaction (PCR)-based method applied to paraffin-embedded tissues. Twenty-two of 45 (49%) cases showed a positive signal determined by direct visualization on gel stained with ethidium bromide. Fourteen of these cases were confirmed by dot blot hybridization, which accords with a prevalence of HPV 16 infection in 31% of Stockholm patients with CIN II-III. The latter value is lower than that published elsewhere (Young et al. 1989; Maki et al. 1991; Skydberg et al. 1991).
Hasuo
Y
Y
Department of Obstetrics and Gynecology, Kurume University School of Medicine, Japan.
Chua
K L
KL
Hjerpe
A
A
D001483:Base Sequence; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013548:Sweden / Q000453:epidemiology; D014412:Tumor Virus Infections / Q000150:complications / Q000453:epidemiology*; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology