title: Comparison of high-performance liquid chromatography with radioimmunoassay for the determination of domoic acid in biological samples.
abstract: A reversed-phase liquid chromatographic method employing UV absorption detection at 242 nm was compared to a radioimmunoassay technique for the determination of the marine toxin, domoic acid, in several types of seafood and biological samples. Agreement between the two methods for spiked samples of mussels and rat serum was very good over a range of concentrations of 0.15-7.3 micrograms/g domoic acid. Also, a very good correlation was observed between the two methods for naturally incurred residues of domoic acid in razor clams, anchovies and crab meat over a concentration range of 0.6-43 micrograms/g domoic acid.
authors.affiliation: Bureau of Chemical Safety, Food Directorate, Banting Research Centre, Ottawa, Ontario, Canada.
authors.forename: J F
authors.initials: JF
authors.lastname: Lawrence
authors.forename: C
authors.initials: C
authors.lastname: Cleroux
authors.forename: J F
authors.initials: JF
authors.lastname: Truelove
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D008387:Marine Toxins / Q000032:analysis* / Q000097:blood / Q000652:urine; D011863:Radioimmunoassay; D051381:Rats; D017747:Seafood / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
chemical_list: D008387:Marine Toxins; C012301:domoic acid; D007608:Kainic Acid