A common precursor to neurotensin and LANT6 and its differential processing in chicken tissues.
Antisera towards neurotensin (NT) and the structurally related peptide, LANT6, were used to characterize immunoreactive peptides and proteins in extracts of chicken tissues. A 17 kDa protein was identified by Western blotting as a potential precursor to NT and LANT6. However, the posttranslational processing of this common precursor appeared to be tissue specific, giving rise to disproportionate amounts of NT and LANT6, along with varying expression of a large molecular LANT6 (M(r), 15 kDa). The intestinal cells containing immunoreactive NT, LANT6, and large molecular LANT6 behaved similarly during fractionation by size and density. These activities also banded together in particles resembling vesicles during centrifugation of isotonic homogenates of tissue. These results suggest that chicken NT and LANT6 are biosynthesized as parts of the same precursor, the processing of which can give rise to a variety of products stored within secretory vesicles.
Carraway
R E
RE
Department of Physiology, University of Massachusetts Medical School, Worcester 01655.
Mitra
S P
SP
Duke
G E
GE
D000818:Animals; D015153:Blotting, Western; D002645:Chickens / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009496:Neurotensin / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011863:Radioimmunoassay; D013347:Subcellular Fractions / Q000737:chemistry; D014018:Tissue Distribution
D009842:Oligopeptides; D011498:Protein Precursors; D009496:Neurotensin; C037243:neurotensin-related hexapeptide