Enterostatin: a gut-brain peptide regulating fat intake in rat.
The effect of enterostatin on high-fat food intake has been investigated. After 18 h of food deprivation, rats were injected intravenously with enterostatin (VPGPR). A dose of 38 nmol of enterostatin gave a significant inhibition of high-fat food intake, while at a higher dose of 76 nmol the inhibiting effect was lost. During the first hour, after injection of enterostatin, there was even a slight increase in food intake. Binding studies of tritiated enterostatin to crude brain membranes indicated one binding site with high affinity (Kd = 0.5 nM) and one with low affinity (Kd = 170 nM). The two dissociation constants suggest different receptor subtypes and could explain why enterostatin both can inhibit and, at high doses, stimulate fat intake in rats.
Sörhede
M
M
Department of Medical and Physiological Chemistry 4, University of Lund, Sweden.
Mei
J
J
Erlanson-Albertsson
C
C
D000818:Animals; D003089:Colipases / Q000494:pharmacology*; D004041:Dietary Fats / Q000008:administration & dosage*; D004792:Enzyme Precursors; D005247:Feeding Behavior / Q000187:drug effects*; D005260:Female; D009479:Neuropeptides / Q000494:pharmacology*; D011485:Protein Binding; D011498:Protein Precursors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D003089:Colipases; D004041:Dietary Fats; D004792:Enzyme Precursors; D009479:Neuropeptides; D011498:Protein Precursors; C037107:procolipase