IFN-gamma stimulates IgG2 production from bovine B cells costimulated with anti-mu and mitogen.
The effect of interferon-gamma (IFN-gamma) antibody production by anti-mu- or pokeweed mitogen-stimulated bovine B cells was studied. IFN-gamma induced IgG2 secretion in isolated bulk B cell populations and in B cells sorted for IgM expression. IgM production was suppressed by the presence of IFN-gamma alone but this effect was antagonized by interleukin 2 (IL2). The effects of IFN-gamma on secreted levels of IgM, IgG1, and IgG2 correlated with the frequencies of cells expressing transcripts of the respective isotypes when stimulated with IFN-gamma-containing T cell supernatants. These results indicate that IFN-gamma plays a key role in IgG2 production in the bovine by directly affecting suitably stimulated B cells. The ability of IL2 to synergize with IFN-gamma to augment both the IgM and IgG2 responses implicates a TH1-like subset in regulation of this isotype.
Estes
D M
DM
University of Missouri, College of Veterinary Medicine, Department of Veterinary Microbiology, Columbia 65211.
Closser
N M
NM
Allen
G K
GK
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D001483:Base Sequence; D002417:Cattle; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics; D007075:Immunoglobulin M / Q000276:immunology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007376:Interleukin-2; D008934:Mitogens; D012333:RNA, Messenger; C044203:anti-IgM; D007371:Interferon-gamma