BK and JC viruses in human immunodeficiency virus type 1-infected persons: prevalence, excretion, viremia, and viral regulatory regions.
Human polyomavirus BK (BKV) and JC (JCV) infections were examined in persons infected with human immunodeficiency virus type 1 (HIV-1). High frequencies of BKV (24%) and JCV viruria (16%) were detected by polymerase chain reaction (PCR). BKV viruria was not found in an immunocompetent control group, in contrast to a frequency of JCV viruria of 20%. The degree of HIV-induced immunodeficiency did not influence the prevalence of BKV viruria, in contrast to cytomegalovirus viruria, suggesting BKV reactivation is an early manifestation in HIV infection as well as a temporal sequence of opportunistic infections. BKV DNA but not JCV DNA was detected in peripheral blood mononuclear cells (PBMC) in 2 of 42 subjects by a sensitive nested PCR. Sequencing of viral noncoding control regions (NCCRs) revealed predominantly archetypal and TU type BKV NCCRs but only archetypal JCV NCCRs. A new, naturally occurring BKV NCCR variant was detected in 1 urine specimen and 2 PBMC samples, indicating a stable and biologically significant rearrangement. Serum levels of BKV antibodies do not seem to be diagnostically useful in HIV-infected persons.
Sundsfjord
A
A
Department of Virology, University of Tromsø, Norway.
Flaegstad
T
T
Flø
R
R
Spein
A R
AR
Pedersen
M
M
Permin
H
H
Julsrud
J
J
Traavik
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D001739:BK Virus / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology; D001483:Base Sequence; D003587:Cytomegalovirus / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015658:HIV Infections / Q000150:complications*; D015497:HIV-1; D006801:Humans; D007577:JC Virus / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000097:blood / Q000150:complications / Q000453:epidemiology* / Q000652:urine; D015995:Prevalence; D014412:Tumor Virus Infections / Q000097:blood / Q000150:complications / Q000453:epidemiology* / Q000652:urine; D014766:Viremia / Q000382:microbiology
D000914:Antibodies, Viral; D004279:DNA, Viral