[Prostaglandin D2-synthase from cattle brain: purification and physico-chemical properties].
Prostaglandin D2-synthase has been purified from bovine brain for the first time and characterized. The enzyme has a molecular mass of 21 kDa and a pH optimum at 9.0 and is activated by dithiothreitol. The presence in the enzyme molecule of carbohydrates and lipids has been established and a preliminary conclusion made about the qualitative composition of the lipids. The ability of the enzyme to form aggregates under the influence of the detergent has been demonstrated. The role of the lipid component in the preservation of the enzyme monomeric structure as well as the possible mechanisms of action of the above inhibitors and activators are discussed.
Avakian
S A
SA
Boiadzhian
A S
AS
Pogosian
A G
AG
Karagezian
K G
KG
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D006863:Hydrogen-Ion Concentration; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000302:isolation & purification* / Q000378:metabolism; D054834:Lipocalins; D008970:Molecular Weight
D054834:Lipocalins; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase
Prostaglandin D2-sintaza iz mozga byka: ochistka i fiziko-khimicheskie svoĭstva.