title: Morphological and functional differentiation of cryopreserved lactating bovine mammary cells cultured on floating collagen gels.
abstract: Cryopreserved bovine mammary epithelial cells prepared from lactating mammary tissue synthesize and secrete the milk proteins alpha s1-casein, lactoferrin (Lf), and alpha-lactalbumin during in vitro culture on collagen gels in serum-free medium. Each milk protein is differently regulated by detachment and thickness of the collagen substratum, fetal calf serum, and prolactin in the medium. Collagen detachment did not modulate lactoferrin secretion but strongly induced casein secretion, with detachment on day 6 (after formation of cell sheets) inducing casein secretion to 3 micrograms/ml medium, which was 2-3-fold higher than for cells on collagen detached on day 2 (prior to cell spreading to form sheets), and ten-fold higher than for cells grown on collagen not detached. Alpha-lactalbumin secretion was also induced, but only to low levels, in cells grown on detached but not on attached collagen. Cells grown on thin collagen gels secreted lower levels of lactoferrin and casein compared to cells on thick collagen. Lactoferrin but not casein secretion was increased in cells grown in the presence of fetal calf serum. Casein but not lactoferrin secretion was completely dependent on prolactin. Cells grown serum-free on collagen gels detached on day 6 of culture showed a polarized epithelial cell layer with high differentiation evidenced by the apical microvilli, tight junctions, and fat droplets surrounded by casein-containing secretory vesicles. An underlying layer of myoepithelial-like cells was also evident. These studies show for cryopreserved primary bovine mammary cells prepared from lactating mammary tissue the induction of highly differentiated and polarized cell morphology and ultrastructure with concomitant induction of the secretion of casein, lactoferrin, and alpha-lactalbumin in vitro, and that the non-coordinate regulation of milk protein secretion by substratum, prolactin, and serum likely involves alternate routing and control of secretion pathways for casein and lactoferrin.
authors.affiliation: Biology Department, American University of Beirut, Lebanon.
authors.forename: R S
authors.initials: RS
authors.lastname: Talhouk
authors.forename: R L
authors.initials: RL
authors.lastname: Neiswander
authors.forename: F L
authors.initials: FL
authors.lastname: Schanbacher
mesh_terms: D000818:Animals; D002364:Caseins / Q000096:biosynthesis / Q000378:metabolism; D002417:Cattle / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen; D015925:Cryopreservation; D016895:Culture Media, Serum-Free; D005782:Gels; D007774:Lactation / Q000502:physiology*; D007781:Lactoferrin / Q000096:biosynthesis / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology*; D011388:Prolactin / Q000502:physiology
chemical_list: D002364:Caseins; D016895:Culture Media, Serum-Free; D005782:Gels; D011388:Prolactin; D003094:Collagen; D007781:Lactoferrin