Structural elucidation of the capsular polysaccharide expressed by Escherichia coli O20:K83:H26 by high resolution NMR spectroscopy.
The structure of the capsular polysaccharide produced by Escherichia coli O20:K83:H26 was investigated by one- and two-dimensional 1H and 13C NMR spectroscopy, and by glycose and methylation analysis. The capsular polysaccharide was shown to be comprised of linear pentasaccharide repeating units with the structure: [formula: see text]
Whittaker
D V
DV
School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
Parolis
L A
LA
Parolis
H
H
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000378:metabolism*; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D002247:Carbon Isotopes; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D006859:Hydrogen