Role of the phosphotransferase system in Escherichia coli strains deficient in hexose phosphate transport.
In Escherichia coli, glucose 6-phosphate is transported via the Uhp system which is inducible by glucose 6-phosphate. We showed that, in a uhp-deficient strain, glucose 6-phosphate was dephosphorylated in the periplasm and that the resulting glucose was subsequently transported into the cells via the phosphotransferase system. The uptake of glucose generated from glucose 6-phosphate allowed the bacteria to produce an increased level of cAMP compared to cells grown on non-limiting concentrations of glucose.
Dumay
V
V
Unité de Régulation de l'Expression Génétique (Centre National de la Recherche Scientifique Unité Associée 1129), Institut Pasteur, Paris, France.
Crasnier
M
M
D000135:Acid Phosphatase / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D001692:Biological Transport; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000502:physiology; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D003470:Culture Media; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D005958:Glucosephosphates; D009004:Monosaccharide Transport Proteins; C489848:UhpA protein, E coli; C080370:UhpA protein, Bacteria; D019298:Glucose-6-Phosphate; D000242:Cyclic AMP; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000262:Adenylyl Cyclases