Orthogonal arrays of particles (OAPs) in perivascular astrocytes and tight junctions in endothelial cells. A comparative study in developing and adult brain microvessels.
The plasmamembranes of the astrocyte processes, which envelop the capillaries of the adult brain, contributing to the blood-brain barrier constitution, are characterized by peculiar aggregates of intramembrane particles (IMPs) packed in orthogonal arrays (orthogonal arrays of particles, OAPs). With the aim of investigating the maturation sequence of the cerebral microvasculature, the IMPs distribution has been analysed in the plasmamembranes of both perivascular astrocytes and endothelial cells of fractured microvessels, in 16-, 20-, 21-day chick embryo and 10-day chicken optic tecta. The IMPs distribution undergoes remarkable changes from late embryonic to early postnatal life in the astrocytes and endothelial cells as well. In the astrocyte plasmamembranes, short chains of individual particles and linear units of packed ones precede the appearance of complete OAPs; in the endothelium junctional plasmamembranes, fibrils of fused particles precede the formation of fibrillary networks which express the tight junction setting up. The parallel formation of the astrocytic OAPs and the endothelial tight junctions further supports the suggestion that mutual relationships establish between perivascular glia and endothelium in the cerebral microvasculature differentiation during the blood-brain barrier development.
Nico
B
B
Institute of Human Anatomy, Histology and Embryology, University of Bari Medical School, Italy.
Cantino
D
D
Sassoé Pognetto
M
M
Bertossi
M
M
Ribatti
D
D
Roncali
L
L
D000818:Animals; D001253:Astrocytes / Q000648:ultrastructure*; D001921:Brain / Q000098:blood supply* / Q000254:growth & development / Q000648:ultrastructure*; D002462:Cell Membrane / Q000648:ultrastructure; D002642:Chick Embryo; D002645:Chickens; D005614:Freeze Fracturing; D007365:Intercellular Junctions / Q000648:ultrastructure*; D008833:Microcirculation / Q000648:ultrastructure; D008854:Microscopy, Electron