Mast cells and histamine release in Crohn's disease.
To study the role of intestinal mast cells in Crohn's disease, a sensitive glass-fiber histamine assay was conducted in conjunction with mechanical dispersion of surgical specimens of 80 macroscopically actively inflamed colons, 40 non-inflamed colons, 40 actively inflamed ileums, and 16 non-inflamed ileums from patients with Crohn's disease and 96 control subjects. A strong correlation was found between the number of mast cells and the total histamine content in the controls (r = 0.682) (p < 0.05). The number of mast cells was decreased in Crohn's disease as compared with the controls (p < 0.01). Intestinal mast cells release histamine in a dose-dependent manner after challenges with anti-IgE (1.875-240.0 U/ml). A significant difference was noted in the release by anti-IgE between actively inflamed and non-inflamed colons of patients with Crohn's disease or control subjects (p < 0.01). Mast cells in actively inflamed tissue with Crohn's disease were shown to have different roles in the pathogenesis of inflammation.
Araki
Y
Y
Department of Surgery, Kurume University School of Medicine, Japan.
Kakegawa
T
T
Stadil
F
F
D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D003424:Crohn Disease / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D006801:Humans; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*
D000888:Antibodies, Anti-Idiotypic; C026408:anti-IgE antibodies; D006632:Histamine