The formation of a dimeric product of raffinose by the action of galactose oxidase.
1. Galactose oxidase is known to catalyze the oxidation of the C-6 hydroxymethyl group of galactose to an aldehyde group. 2. When the products of a galactose oxidase-catalase treatment of raffinose were examined by gel filtration and ion exchange chromatography, we found that in addition to the expected 6"-aldehydroaffinose, two other components were present. 3. One component was neutral and had an elution volume close to that of maltopentaose and on treatment with sodium borohydride or hypoiodite, this component was converted to raffinose or 6"-carboxyraffinose, respectively. 4. Fast atom bombardment mass spectroscopy in the negative mode indicated that the major molecular ion had an M/Z of 1003. 5. These data are consistent with this component being a dimer of 6"-aldehydoraffinose.
Kelleher
F M
FM
Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Bhavanandan
V P
VP
Reinhold
V N
VN
D002384:Catalysis; D004187:Disaccharides / Q000737:chemistry*; D005692:Galactose Oxidase; D011887:Raffinose / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D004187:Disaccharides; D005692:Galactose Oxidase; D011887:Raffinose