title: Venous blood arterialization and parameters from the minimal models of glucose disappearance and post-hepatic insulin delivery.
abstract: 1. Modelling analysis of intravenous glucose tolerance test glucose and insulin concentrations can provide measures of insulin sensitivity and metabolism from a single straightforward procedure. However, little is known of the effects of blood arterialization on model-derived parameters. 2. Intravenous glucose tolerance tests were carried out on 18 subjects, with measurement of glucose and insulin concentrations in simultaneously sampled non-arterialized and arterialized blood. Blood oxygen saturation, partial pressure of CO2 and pH were measured on both non-arterialized and arterialized blood during the intravenous glucose tolerance test. Using the minimal models of glucose disappearance and post-hepatic insulin delivery, measures of insulin sensitivity, glucose-dependent glucose disposal, first- and second-phase post-hepatic insulin responsiveness to glucose and plasma insulin elimination rate were derived from intravenous glucose tolerance test glucose and insulin concentrations in both arterialized and non-arterialized blood. 3. During the intravenous glucose tolerance test mean blood oxygen saturation was 6.7% higher, partial pressure of CO2 was 0.3 kPa lower and pH was 0.015 higher in arterialized than non-arterialized blood. Mean parameter values did not differ when derived from measurements made on non-arterialized and arterialized blood. Model-derived parameters were not related to the degree of arterialization, although there was some consistent variation with sampling site for parameters of glucose-dependent glucose disposal (Sg), first-phase post-hepatic insulin responsiveness (phi 1) and insulin elimination (ni). 4. Measurements made on non-arterialized blood are suitable for analyses employing the minimal models of glucose disappearance and post-hepatic insulin delivery. Imprecision in some parameters may be diminished by adherence to a single sampling site.
authors.affiliation: Wynn Institute for Metabolic Research, London, U.K.
authors.forename: I F
authors.initials: IF
authors.lastname: Godsland
authors.forename: C
authors.initials: C
authors.lastname: Felton
authors.forename: V
authors.initials: V
authors.lastname: Wynn
mesh_terms: D000328:Adult; D001158:Arteries; D001769:Blood / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism*; D001831:Body Temperature; D002245:Carbon Dioxide / Q000097:blood; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D010100:Oxygen / Q000097:blood; D014680:Veins
chemical_list: D001786:Blood Glucose; D007328:Insulin; D002245:Carbon Dioxide; D010100:Oxygen