Induction of enoyl-CoA hydratase by LD50 exposure to perfluorocarboxylic acids detected by two-dimensional electrophoresis.
The effect of in vivo exposure to perfluoro-n-octanoic and perfluoro-n-decanoic acids was examined in the rat liver by two-dimensional electrophoresis (2DE). Using nonequilibrium pH-gradient electrophoresis in the first dimension separation, proteins associated with the mitochondrial/peroxisomal cell fraction were observed and immunologically identified. Conspicuous inductions in peroxisomal enoyl-CoA hydratase and other proteins of the peroxisomal beta-oxidative pathway were observed following single-dose exposure to each compound. The abundance of the tentatively-identified mitochondrial equivalent, crotonase, was not altered by these intoxications. These results confirm previous observations of perfluorocarboxylic acid toxicity and support the use of 2D protein-pattern alterations in biomarker research. The ability to identify this type of alteration via 2DE, in association with specific toxic effects by chemically related compounds, may provide new and additional markers for chemical-induced tissue damage.
Witzmann
F A
FA
Department of Biology, Indiana University-Purdue University at Columbus 47203.
Parker
D N
DN
Jarnot
B M
BM
D000818:Animals; D002210:Caprylates / Q000008:administration & dosage / Q000633:toxicity*; D003652:Decanoic Acids / Q000008:administration & dosage / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D004746:Enoyl-CoA Hydratase / Q000096:biosynthesis* / Q000187:drug effects / Q000302:isolation & purification; D004790:Enzyme Induction / Q000187:drug effects; D005466:Fluorocarbons / Q000008:administration & dosage / Q000633:toxicity*; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
D002210:Caprylates; D003652:Decanoic Acids; D005466:Fluorocarbons; C036567:perfluorodecanoic acid; C023036:perfluorooctanoic acid; D004746:Enoyl-CoA Hydratase