A new human slow skeletal troponin T (TnTs) mRNA isoform derived from alternative splicing of a single gene.
A human muscle cDNA library was screened for slow skeletal troponin T (TnTs). Sequence analysis revealed that one of the selected clones had a cDNA coding sequence different from the two previously described. RNase protection assays confirmed the expression of this new isoform in adult skeletal muscle. In addition, results of the RNase protection assays strongly suggested the expression of a fourth isoform. These isoforms for human slow TnT most likely result from combinatorial alternative splicing of a single gene.
Samson
F
F
Faculté de Médecine Paris-Sud, C.N.R.S. URA 1159, Department of Medical Research, Hôpital Marie Lannelongue, Le Plessis Robinson, France.
Mesnard
L
L
Mihovilovic
M
M
Potter
T G
TG
Mercadier
J J
JJ
Roses
A D
AD
Gilbert
J R
JR
D000328:Adult; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015342:DNA Probes; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D012260:Ribonucleases; D014336:Troponin / Q000096:biosynthesis* / Q000235:genetics*; D020107:Troponin T
D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D014336:Troponin; D020107:Troponin T; D012260:Ribonucleases