Crude or recombinant proteins applied to latex agglutination, complement fixation and enzyme-linked immunosorbent assays for the serodiagnosis of invasive amebiasis.
Three different agglutination tests were developed for the detection of serum antibodies against Entamoeba histolytica. These tests are based on carboxylated polystyrene beads loaded either with a purified recombinant E. histolytica protein, designated recEh-P1, or a soluble fraction, or a membrane fraction (M-LA) both prepared from E. histolytica trophozoites. The three agglutination tests were compared with an enzyme-linked immunosorbent assay and a complement fixation test based on crude soluble E. histolytica antigens as well as with an enzyme-linked immunosorbent assay using recEh-P1 as antigen (P1-EIA). Serum samples from patients with invasive amebiasis (n = 30), or infectious diseases unrelated to E. histolytica (n = 57), as well as sera of apparently healthy individuals (n = 25) including some with noninvasive amebiasis (n = 5) were analysed by all six methods. Depending on the assay used, the results obtained, revealed sensitivities ranging from 83% to 100% and specificities ranging from 93% to 100%. P1-EIA and M-LA exhibited best results, both with a sensitivity of 100% and a specificity of 98%.
Lotter
H
H
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Mannweiler
E
E
Tannich
E
E
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology; D003168:Complement Fixation Tests; D004748:Entamoeba histolytica / Q000737:chemistry / Q000276:immunology*; D004749:Entamoebiasis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006801:Humans; D007841:Latex Fixation Tests; D008863:Microspheres; D015800:Protozoan Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D011994:Recombinant Proteins