title: The developmental increase in ACh current densities on rat sympathetic neurons correlates with changes in nicotinic ACh receptor alpha-subunit gene expression and occurs independent of innervation.
abstract: Determining factors that control the expression of neurotransmitter receptors and the mechanisms by which these factors operate is essential to understand how synapses form during development and how receptor numbers change in the adult. In this study, we have investigated one such factor, the influence of innervation, on the developmental expression of nicotinic ACh receptors (nAChRs) on neonatal rat sympathetic neurons, both in terms of ACh current densities, and in terms of mRNA levels for the transcripts that encode these receptors. To date, nine genes have been cloned that encode neuronal nAChRs subunits in mammals. We demonstrate that mRNA encoding five nAChR subunits, alpha 3, alpha 5, alpha 7, beta 2, and beta 4, are present in neonatal rat sympathetic neurons. We show that mRNA levels for alpha 3 and alpha 7 subunits increase by more than threefold over the first 2 postnatal weeks, a period when most synapses are forming on the neurons; however, we observed no significant change in mRNA levels for alpha 5, beta 2, or beta 4. Using whole-cell voltage-clamp techniques, we show that the increase in alpha-subunit mRNA correlates with increases in ACh current densities, which double over the same period. To investigate the role of innervation, we cut the preganglionic nerve at birth and measured subunit mRNA levels and ACh current densities in denervated neurons 1-2 weeks later. Our results indicate that the preganglionic nerve differentially affects the mRNA level for the five nAChR transcripts, yet it has little influence on the developmental increase in ACh current densities.
authors.affiliation: Department of Physiology, McGill University, Montréal, Québec, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Mandelzys
authors.forename: B
authors.initials: B
authors.lastname: Pié
authors.forename: E S
authors.initials: ES
authors.lastname: Deneris
authors.forename: E
authors.initials: E
authors.lastname: Cooper
mesh_terms: D000109:Acetylcholine / Q000502:physiology*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D003001:Cloning, Molecular; D003714:Denervation; D005786:Gene Expression Regulation; D046911:Macromolecular Substances; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011978:Receptors, Nicotinic / Q000096:biosynthesis* / Q000502:physiology; D017783:Superior Cervical Ganglion / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D046911:Macromolecular Substances; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D000109:Acetylcholine