Simultaneous separation of inorganic cations and anions by ion chromatography using a single column coated with weak/strong-charged zwitterionic bile salt micelles.
A single reversed-phase ODS (octadecyl silica) column coated with taurine-conjugated bile salt micelles has been investigated for simultaneous separation of inorganic cations and anions. Under acidic conditions in the separation column, taurine-conjugated bile salts are protonated; therefore, the stationary phase coated with taurine-conjugated bile micelles exists as a "weak/strong"-charged zwitterionic stationary phase (H+ and SO3-). The weak/strong-charged zwitterionic stationary phase provides a new retention mechanism which is different from conventional ion chromatography in that both positive and negative charges are close together in a single molecule, resulting in simultaneous electrostatic attraction and repulsion of the analyte ions. Also, the stronger negative charge (sulfonate) of the stationary phase works as a cation-exchange site at the same time. The combined effects of cation-exchange and simultaneous electrostatic repulsion/attraction interactions were successfully used in ion chromatography for the simultaneous separation of inorganic cations and anions.
Hu
W
W
Department of Applied Chemistry, School of Engineering, Nagoya University, Japan.
Haraguchi
H
H
D000838:Anions / Q000302:isolation & purification*; D001647:Bile Acids and Salts / Q000737:chemistry*; D002412:Cations / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D008823:Micelles
D000838:Anions; D001647:Bile Acids and Salts; D002412:Cations; D008823:Micelles