title: Colocalization of calbindin-D28k with vasopressin in hypothalamic cells of the rat: a double-labeling immunofluorescence study.
abstract: By use of a double-labeling immunofluorescence method, we examined whether vasopressin-containing cells possess a calcium-binding protein, calbindin-D28k, in the hypothalamus of the rat. Subpopulations of vasopressin-containing cells varied in their ability to possess calbindin-D28k immunoreactivity in different regions. In the supraoptic nucleus, most vasopressin-immunoreactive cells were also stained for calbindin-D28k. By contrast, in the magnocellular part of the hypothalamic paraventricular nucleus, all vasopressin-labeled cells lacked calbindin-D28k. In the suprachiasmatic nucleus, no calbindin-D28k was found in vasopressin-stained cells. This study shows a further characterization of vasopressin-containing cells of the rat hypothalamus.
authors.affiliation: Department of Anatomy, Fujita Health University School of Medicine, Aichi, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Arai
authors.forename: D M
authors.initials: DM
authors.lastname: Jacobowitz
authors.forename: S
authors.initials: S
authors.lastname: Deura
mesh_terms: D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D005455:Fluorescent Antibody Technique; D007031:Hypothalamus / Q000166:cytology*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D013493:Suprachiasmatic Nucleus / Q000166:cytology; D013495:Supraoptic Nucleus / Q000166:cytology; D014667:Vasopressins / Q000032:analysis*
chemical_list: C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D014667:Vasopressins