An improved method for the measurement of malondialdehyde in biological samples.
An improved method was developed for measuring malondialdehyde (MDA) as its thiobarbituric acid (TBA) complex. Samples were initially incubated with 1% potassium iodide and 0.1% butylated hydroxytoluene at 50 degrees C for 20 min, and then with 0.4% TBA at 60 degrees C for 60 min. The MDA-TBA complex formed was extracted with isobutyl alcohol and measured by high-performance liquid chromatography with fluorescence detection. The improved method allows for a more specific determination of MDA present in biological samples.
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Department of Nutrition and Food Science, University of Kentucky, Lexington 40506-0054.
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D002623:Chemistry Techniques, Analytical / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis* / Q000097:blood; D013858:Thiobarbiturates / Q000737:chemistry
D013858:Thiobarbiturates; D008315:Malondialdehyde; C029684:thiobarbituric acid