Synthesis of novel tellurium containing analogues of choline and acetylcholine and their quantitation by pyrolysis-gas chromatography-mass spectrometry.
Methods for the synthesis and quantitation of the novel choline analogues, telluronium choline and acetyltelluronium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with [2H4]telluronium choline and [2H4]acetyltelluronium choline as internal standards. The telluronium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 232 and m/z 190 for acetyltelluronium and telluronium choline, respectively, and at m/z 236 and m/z 194 for the analogous deuterated internal standards. The assay was linear over a range of 20 pmol-20 nmol of compound taken through the assay.
Harris
S E
SE
Department of Chemistry and Biochemistry, University of South Carolina, Columbia 29208.
Silks
L A
LA
Dunlap
R B
RB
Odom
J D
JD
Kosh
J W
JW
D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009942:Organometallic Compounds / Q000032:analysis / Q000138:chemical synthesis*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013691:Tellurium / Q000032:analysis*
D007202:Indicators and Reagents; D009942:Organometallic Compounds; C086332:acetyltelluronium choline; C086331:d(0)-telluronium choline; D013691:Tellurium