Activation of the gene for type-b natriuretic factor in mouse stem cell cultures induced for cardiac myogenesis.
We assessed the temporal transcriptional activity profiles of the genes for type-B natriuretic factor, BNF, the isoform ANF, and other cardiac muscle proteins in differentiating cultures derived from multipotential mouse cell lines. P19 embryonal carcinoma cells and D3 embryonic stem cells were induced for in vitro cardiac myogenesis; RNA was isolated at regular intervals throughout the differentiation programs, and mRNAs were detected by reverse transcriptase mediated polymerase chain reactions. The transcriptional activation profiles of the ANF and BNF gene were similar, but there were quantitative differences that were best assayed by use of competitive internal DNA standards. The levels of induced BNF transcripts were highest in the P19 developmental system reaching approximately 10% of adult mouse ventricular muscle levels; those for ANF were lower, but also readily detected. The cell lines may be used to define the regulatory control elements for natriuretic factor gene expression, in stably transfected cell lines, during cardiac muscle growth.
Boer
P H
PH
University of Ottawa Heart Institute, Department of Pathology & Laboratory Medicine, Ontario, Canada.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D018076:DNA, Complementary / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology; D006321:Heart / Q000196:embryology*; D015850:Interleukin-6; D007438:Introns; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000166:cytology; D020097:Natriuretic Peptide, Brain; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013724:Teratoma; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D012333:RNA, Messenger; D011994:Recombinant Proteins; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor