[Relative risk analysis of the development of cervical noncancer lesion and cervical carcinoma associated with HPV infection].
Two-hundred and fourty-seven DNA samples extracted from cervical carcinoma tissues and 143 from cervical noncancer lesions were examined for the presence of human papillomavirus (HPV) type 6B, 11, 16 and 18 DNA sequences by means of dot blot hybridization using 32P-dCTP-labelled HPV probes (Tm-17 degrees C). The result showed that the overall positive rate of HPV infection in this group of cervical cancer was 65.99%, HPV type 16 was dominantly found in the group (55.06%) and accounted for 71.28% in all the positive samples of cervical cancers, which were 4.25 and 5.67 times higher than those of HPV type 6/11 and 18, respectively. Detectable sequences homologous to HPVs in the cervical noncancer group was 51.05% and HPV types 6/11 accounted for 50.61% in the HPV-positive samples. The relative risk analysis of HPV infection in the two groups was performed and the relative risk (RR) of HPV infection for development of cervical cancer was increased (RR = 1.86, P < 0.01). Further analysis revealed that HPV 16, 18 infection in cervical epithelium might be the higher risk factor in the development of cervical carcinoma (RR = 3.43, P < 0.001); whereas HPV type 6/11 might be the lower risk factor for the development of cervical cancer (RR = 0.5, P < 0.05): Our results implied that the development of cervical cancer may be closely associated with HPV infection, especially with HPV type 16 and 18.
Zhang
W
W
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
D002584:Cervix Uteri / Q000382:microbiology*; D002681:China / Q000453:epidemiology; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology*; D012307:Risk Factors; D014412:Tumor Virus Infections / Q000453:epidemiology*; D002577:Uterine Cervical Diseases / Q000382:microbiology; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000382:microbiology*