Mineral interactions in rats fed AIN-76A diets with excess calcium.
The effects of moderate increases in dietary calcium on maternal and foetal mineral interactions were studied in Charles River CD/VAF Plus rats. Female rats were given 0.50, 0.75, 1.00 or 1.25% dietary calcium as calcium carbonate in AIN-76A diets for 6 wk before mating, during mating and for 20 days of gestation. Inductively coupled argon plasma-atomic emission spectrometry was used to determine mineral levels in the tissues of non-pregnant rats after 42 days on the diets, in the tissues of pregnant rats on day 20 of gestation and in the whole body of day-20 foetuses. The femurs of the non-pregnant and pregnant rats had a dose-related linear increase in calcium content. In livers of the non-pregnant rats, dose-related linear increases in the phosphorus, zinc and magnesium content were observed, but there was a dose-related decrease in the iron content. There were dose-related linear decreases in the iron and copper contents of the kidneys from the non-pregnant rats. In pregnant rats dose-related linear decreases were observed in the iron content of the liver and in the zinc, iron and magnesium contents of the kidney. The foetuses from rats given a moderate increase in dietary calcium had dose-related decreases in the whole-body contents of phosphorus, iron, copper and magnesium.
Shackelford
M E
ME
Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Washington, DC 20204.
Collins
T F
TF
Black
T N
TN
Ames
M J
MJ
Dolan
S
S
Sheikh
N S
NS
Chi
R K
RK
O'Donnell
M W
MW
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002136:Calcium, Dietary / Q000008:administration & dosage*; D003300:Copper / Q000378:metabolism; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005260:Female; D005269:Femur / Q000378:metabolism; D005333:Fetus / Q000378:metabolism*; D005865:Gestational Age; D007501:Iron / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008903:Minerals / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D051381:Rats; D013052:Spectrometry, X-Ray Emission; D015032:Zinc / Q000378:metabolism
D002136:Calcium, Dietary; D008903:Minerals; D010758:Phosphorus; D003300:Copper; D007501:Iron; D008274:Magnesium; D015032:Zinc; D002118:Calcium