title: Crystallization and preliminary X-ray analysis of Candida albicans phosphomannose isomerase.
abstract: Crystals of recombinant phosphomannose isomerase from Candida albicans have been obtained in a form suitable for X-ray diffraction analysis. The enzyme plays a key role in the biosynthesis of the mannan component of the fungal cell wall. It crystallizes in monoclinic space group C2, with cell dimensions a = 124.9 A, b = 52.9 A, c = 85.9 A and beta = 127.4 degrees. The crystals diffract to Bragg spacings beyond 1.7 A, native data have been collected to 2.4 A and a search for heavy-metal derivatives is in progress. The asymmetric unit contains one molecule of the enzyme (M(r) approximately 49,000) with a Vm of 2.3 A3/Da.
authors.affiliation: epartment of Chemistry, University of York, Heslington, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Tolley
authors.forename: G
authors.initials: G
authors.lastname: Davies
authors.forename: R E
authors.initials: RE
authors.lastname: Hubbard
authors.forename: D J
authors.initials: DJ
authors.lastname: Smith
authors.forename: A E
authors.initials: AE
authors.lastname: Proudfoot
authors.forename: M A
authors.initials: MA
authors.lastname: Payton
authors.forename: A
authors.initials: A
authors.lastname: Cleasby
authors.forename: A
authors.initials: A
authors.lastname: Wonacott
authors.forename: T N
authors.initials: TN
authors.lastname: Wells
mesh_terms: D002176:Candida albicans / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008359:Mannose-6-Phosphate Isomerase / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D011994:Recombinant Proteins; D008359:Mannose-6-Phosphate Isomerase