title: Evaluation of capillary supercritical fluid chromatography with mass spectrometric detection for the analysis of a drug (mebeverine) in a dog plasma matrix.
abstract: Supercritical fluid chromatography with mass spectrometric detection was evaluated as a technique for the analysis of drugs in biological fluids. Dog plasma was spiked with a model drug, mebeverine, and with a deuterium-labeled analog of mebeverine. The spiked plasma was prepared for analysis by solid-phase extraction on octadecylsilane cartridges. Mebeverine levels in the spiked dog plasma samples were determined by interpolation from a standard curve. Accuracy and precision of the analysis were determined within and between days. In general, accuracy was found to be 100 +/- 15% for plasma samples spiked with 6 to 60 ng mebeverine/ml. The relative standard deviation for replicate sample analysis over this concentration range was between 5 and 12.5%.
authors.affiliation: Procter and Gamble Company, Miami Valley Laboratories, Cincinnati, OH 45239-8707.
authors.forename: J D
authors.initials: JD
authors.lastname: Pinkston
authors.forename: C J
authors.initials: CJ
authors.lastname: Venkatramani
authors.forename: L J
authors.initials: LJ
authors.lastname: Tulich
authors.forename: D J
authors.initials: DJ
authors.lastname: Bowling
authors.forename: K R
authors.initials: KR
authors.lastname: Wehmeyer
mesh_terms: D000818:Animals; D002845:Chromatography; D003903:Deuterium; D004285:Dogs; D005069:Evaluation Studies as Topic; D013058:Mass Spectrometry; D010276:Parasympatholytics / Q000097:blood*; D010627:Phenethylamines / Q000097:blood*
chemical_list: D010276:Parasympatholytics; D010627:Phenethylamines; C005096:mebeverine; D003903:Deuterium