title: Transcription factor GATA-1-multiprotein complexes and chicken erythroid development.
abstract: The chicken erythrocyte transcription factor, GATA-1, is associated with several non-DNA binding proteins. We show that GATA-1 multiprotein complexes exist in primitive and definitive erythrocytes. These complexes bind to GATA motifs of the rho-globin promoter and histone H5 enhancer with high affinity, and to the chicken beta-globin promoter specialized TATA element and enhancer GATA with low affinity. The low affinity beta-globin TATA element would allow basal transcription factors to displace the GATA-1 multiprotein complex. Further, our results suggest that rho-globin promoter's low affinity Sp1 binding site and reduced levels of Sp1 in definitive cells prevent its expression in these cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
authors.forename: C G
authors.initials: CG
authors.lastname: Penner
authors.forename: J R
authors.initials: JR
authors.lastname: Davie
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D004912:Erythrocytes / Q000737:chemistry; D015672:Erythroid Precursor Cells / Q000737:chemistry; D050981:Erythroid-Specific DNA-Binding Factors; D004920:Erythropoiesis; D005914:Globins / Q000235:genetics; D006657:Histones / Q000235:genetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D006657:Histones; D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D009838:Oligodeoxyribonucleotides; D014157:Transcription Factors; D005914:Globins