title: In human chromosomes telomeric regions are enriched in CpGs relative to R-bands.
abstract: Human chromosomes were in situ nick-translated using as nicking agents the endonucleases MspI (CCGG), its methyl-sensitive isoschizomer HpaII, HaeIII (GGCC), SacII (CCGCGG), EcoRI (GAATTC) and DNaseI. We show that in metaphase chromosomes R-bands are enriched, as compared with G-bands, in the dinucleotide CpG but no more than what is expected on the basis of their relative G+C content. The telomeric regions, on the contrary, besides having a chromatin conformation that is particularly relaxed and accessible to endonucleases, also show an enrichment in CpGs.
authors.affiliation: Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Roma, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Ferraro
authors.forename: V
authors.initials: V
authors.lastname: Predazzi
authors.forename: G
authors.initials: G
authors.lastname: Prantera
mesh_terms: D001345:Autoradiography; D001483:Base Sequence; D002871:Chromosome Banding; D003596:Cytosine; D004262:DNA Restriction Enzymes; D006147:Guanine; D006801:Humans; D008969:Molecular Sequence Data; D016615:Telomere / Q000737:chemistry*
chemical_list: D006147:Guanine; D003596:Cytosine; D004262:DNA Restriction Enzymes