Inhibitory action of palmitic acid on the growth of Saccharomyces cerevisiae.
High concentrations of long-chain fatty acids have been found to be harmful to mammalian cells and prokaryotic organisms. This effect was investigated in Saccharomyces cerevisiae. Addition of 3 mmol/L palmitate to a yeast extract-peptone medium caused a significant inhibition of cell growth during the first 2 d of incubation, followed by renewed growth and palmitate utilization. Inhibition was also observed with palmitate concentrations down to 0.1 mmol/L. As inferred from catalase activity determinations, this effect was found to correlate with the absence of peroxisome proliferation. Finally, no inhibition was observed in exponential-phase cultures or in the presence of 0.1 g/L glucose, this suggesting that the physiological state of the cell may determine whether its growth will be inhibited by fatty acids.
Dell'Angelica
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EC
Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Stella
C A
CA
Ermácora
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MR
Santomé
J A
JA
Ramos
E H
EH
D002374:Catalase / Q000378:metabolism; D003470:Culture Media; D005227:Fatty Acids / Q000378:metabolism; D008830:Microbodies / Q000187:drug effects / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism
D003470:Culture Media; D005227:Fatty Acids; D010169:Palmitic Acids; D019308:Palmitic Acid; D002374:Catalase