Retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and four retinyl esters in serum determined simultaneously by reversed-phase HPLC with multiwavelength detection.
We describe the use of HPLC with multiwavelength detection to measure retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, beta-carotene, and the linoleate, oleate, palmitate, and stearate esters of retinol in a single 200-microL serum sample. The method is sensitive enough to detect individual retinyl esters in fasting serum from a nonhyperlipidemic population and requires only 12 min for each sample. Serum concentration ranges and means are reported for retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and the sum of the retinyl esters from serum analyses of 3480 participants from several different studies.
Sowell
A L
AL
Division of Environmental Health Laboratory Sciences, Centers for Disease Control and Prevention, Public Health Service, Atlanta, GA 30341-3724.
Huff
D L
DL
Yeager
P R
PR
Caudill
S P
SP
Gunter
E W
EW
D002338:Carotenoids / Q000031:analogs & derivatives / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D065366:Cryptoxanthins; D006801:Humans; D014975:Lutein / Q000097:blood; D000077276:Lycopene; D009752:Nutritional Status; D011786:Quality Control; D012016:Reference Values; D012176:Retinoids / Q000097:blood*; D012680:Sensitivity and Specificity; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood*; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene
D065366:Cryptoxanthins; D012176:Retinoids; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D002338:Carotenoids; C041635:alpha-carotene; D000077276:Lycopene; D014975:Lutein