title: Cytogenetic triclonality in acute myeloid leukemia: a morphologic, immunologic and in situ hybridization study.
abstract: Cytogenetically unrelated clones are uncommon in hematologic malignancies. We report a case of acute myeloid leukemia, which consisted morphologically of two populations of small and large blasts demonstrating immunophenotypic heterogeneity. Cytogenetic analysis showed 3 karyotypically abnormal clones: 47,XY, +14/45,XY,dic(5;17)(q11;p11),14dmin, and a near-tetraploid clone. In situ hybridization showed that the near-tetraploid clone corresponded to the large blasts, and the near-diploid clones the small blasts, therefore demonstrating a direct relationship between cell size and DNA content. The diverse morphologic, immunologic and cytogenetic heterogeneity observed in our case suggested hematopoietic oligoclonality.
authors.affiliation: University Department of Medicine, Queen Mary Hospital, Hong Kong.
authors.forename: Y L
authors.initials: YL
authors.lastname: Kwong
authors.forename: C K
authors.initials: CK
authors.lastname: Lam
authors.forename: A Y
authors.initials: AY
authors.lastname: Chan
authors.forename: A K
authors.initials: AK
authors.lastname: Lie
authors.forename: L C
authors.initials: LC
authors.lastname: Chan
mesh_terms: D000368:Aged; D002869:Chromosome Aberrations; D006801:Humans; D016130:Immunophenotyping; D017403:In Situ Hybridization; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male