Diagnosis of genital herpes by polymerase chain reaction amplification.
A new polymerase chain reaction (PCR) method employing type-specific primers and probes was applied to 114 clinical specimens obtained from 58 female patients with genital lesions or who had a history of genital herpes. Ten and 15 specimens, respectively, were positive for herpes simplex virus (HSV)-1 and HSV-2 by cell culture. All of 10 culture-confirmed HSV-1 cases and 11 of 15 (73%) culture-confirmed HSV-2 cases were identified by PCR. Although there were several cases with discrepancy between cell culture and PCR for HSV-2, the results suggest that this PCR procedure could be applied to clinical specimens from the female genital tract.
Goto
T
T
Department of Obstetrics and Gynecology, Tokyo University Branch Hospital, Japan.
Yamaguchi
Y
Y
Hashido
M
M
Yoshikawa
H
H
Kawana
T
T
D001483:Base Sequence; D002478:Cells, Cultured; D005260:Female; D006558:Herpes Genitalis / Q000175:diagnosis*; D018259:Herpesvirus 1, Human / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction