title: A porcine gut polypeptide identical to the pancreatic hormone PP (pancreatic polypeptide).
abstract: A peptide hormone has been isolated from porcine intestine. Its primary structure was found to consist of 36 amino acid residues in a sequence identical to that of the porcine pancreatic polypeptide, previously not isolated from intestines or a tissue other than pancreas. The gut polypeptide significantly suppresses glucose-induced insulin secretion in vitro. Using an immunohistochemical technique, we also identified cells in the porcine gastrointestinal tract that were immunoreactive with pancreatic polypeptide. The immunoreactivity disappeared after absorption with the isolated gut polypeptide or synthetic human pancreatic polypeptide.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Chen
authors.forename: T
authors.initials: T
authors.lastname: Bergman
authors.forename: C G
authors.initials: CG
authors.lastname: Ostenson
authors.forename: A
authors.initials: A
authors.lastname: Höög
authors.forename: J
authors.initials: J
authors.lastname: Näslund
authors.forename: A
authors.initials: A
authors.lastname: Norberg
authors.forename: M
authors.initials: M
authors.lastname: Carlquist
authors.forename: S
authors.initials: S
authors.lastname: Efendic
authors.forename: V
authors.initials: V
authors.lastname: Mutt
authors.forename: H
authors.initials: H
authors.lastname: Jörnvall
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D010191:Pancreatic Polypeptide / Q000737:chemistry / Q000302:isolation & purification*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D013552:Swine
chemical_list: D010455:Peptides; D010191:Pancreatic Polypeptide