title: Restricted VH gene usage by murine hybridomas directed against the human N, but not M, blood group antigen.
abstract: The M and N human blood group antigens are complex glycopeptide determinants at the amino terminus of the red blood cell membrane glycoprotein, glycophorin A. The heavy and light chain variable region cDNA sequences were determined for seven murine monoclonal antibodies recognizing glycophorin A. Three of the antibodies were anti-M and four were anti-N. Each of the anti-M antibodies was composed of VH and VL regions derived from distinct germline gene families (VH1 (J558), VH4 (X24), VH5 (7183), VK5, VK8, and VK19). In contrast, all four anti-N heavy chains were composed of VH regions derived from the VH2 (Q52) germline gene family and all used the same J4 gene segment. In addition, two of the anti-N light chains were composed of VK regions from the VK8 germline gene family and used the J1 gene segment. Since each anti-N hybridoma was derived from different mice immunized by different protocols, these results suggest that the murine immune response to the N, but not the M, human blood group antigen is restricted.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104.
authors.forename: M
authors.initials: M
authors.lastname: Czerwinski
authors.forename: D P
authors.initials: DP
authors.lastname: Blackall
authors.forename: W R
authors.initials: WR
authors.lastname: Abrams
authors.forename: R J
authors.initials: RJ
authors.lastname: Rubocki
authors.forename: S L
authors.initials: SL
authors.lastname: Spitalnik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005803:Genes, Immunoglobulin / Q000235:genetics*; D006021:Glycophorins / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008951:MNSs Blood-Group System / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D006021:Glycophorins; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D008951:MNSs Blood-Group System