[Nucleotide sequence of internal transcribed spacers and 5.8S rDNA for the ribosomal operon from alfalfa Medicago sativa and cotton Gossypium hirsutum L].
The 708- and 769-bp fragments from alfalfa and cotton containing the 3'-end of the 18S gene, the internal transcribed spacer. 1 (ITS1) the 5.8S gene, ITS2, and the 5'-end of the 28S gene were obtained using primers to the 18S and 28S ends of rDNA from tomato by a polymerase chain reaction. These sequences were cloned into pTZ19R. The 5.8S rDNA, ITS1 and ITS2 nucleotide sequences of alfalfa and cotton were determined. Comparative analysis of nucleotide sequences of alfalfa and cotton showed large hypervariability of spacer regions and conservatism of 5.8S rDNA sequence.
Mukhamedov
R S
RS
Martsinkovskaia
A I
AI
Shu
Kh
Kh
Abdukarimov
A A
AA
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics*; D003368:Gossypium / Q000235:genetics*; D000455:Medicago sativa / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D004275:DNA, Ribosomal; D012341:RNA, Ribosomal, 5S
Nukleotidnye posledovatel'nosti vnutrennikh transkribiruemykh speĭserov i 5.8S rDNA ribosomnogo operona u liutserny Medicago sativa i khlopchatnika Gossypium hirsutum L.