title: Can Ki67 immunostaining predict response to radiotherapy in oral squamous cell carcinoma?
abstract: OBJECTIVE
  To determine whether immunohistochemical evaluation of the abatement of proliferating cells after a first course of radiotherapy could predict the final response to treatment in oral squamous cell carcinoma (SCC).
  
  
  METHODS
  Frozen sections from 31 cases of histologically confirmed oral SCC were stained with the monoclonal antibody Ki67 at diagnosis and after 10 Gy of radiotherapy. The percentage difference of Ki67 positive cells among the biopsy specimens taken at the beginning and after 10 Gy was correlated with the clinical response obtained at the end of the treatment and its significance determined.
  
  
  RESULTS
  The percentage of Ki67 positive cells at diagnosis had no significant correlation with the final therapeutic result of radiotherapy. By contrast, the 32% difference of proliferating cells after 10 Gy of radiotherapy significantly differentiated responders from non-responders (p < 0.05). Furthermore, the abatement of the growth fraction after 10 Gy of radiotherapy was significantly correlated with the complete response (p < 0.01).
  
  
  CONCLUSIONS
  These data show that the immunohistochemical evaluation of the abatement of Ki67 positive cells after 10 Gy of radiotherapy provides an independent variable of responsiveness to radiotherapy, allowing a reliable prediction of the final therapeutic result to be made.
authors.affiliation: Department of Biomedical Sciences and Oncology, University of Turin, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Valente
authors.forename: R
authors.initials: R
authors.lastname: Orecchia
authors.forename: S
authors.initials: S
authors.lastname: Gandolfo
authors.forename: M
authors.initials: M
authors.lastname: Arnaudo
authors.forename: R
authors.initials: R
authors.lastname: Ragona
authors.forename: S
authors.initials: S
authors.lastname: Kerim
authors.forename: G
authors.initials: G
authors.lastname: Palestro
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D002455:Cell Division / Q000528:radiation effects; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen; D009062:Mouth Neoplasms / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D011379:Prognosis; D011446:Prospective Studies
chemical_list: D000951:Antigens, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins