In vivo effect of free fatty acids on the specific binding of glucocorticosteroids to corticosteroid binding globulin and liver receptors in immature rats.
Stimulating lipase activity with heparin (200 IU/kg b.w.) increased the plasma free fatty acid (FFA) concentration of immature rats (15 days). The effect of this elevated FFA concentration on glucocorticoid binding to corticosteroid binding globulin (CBG), and liver cytosol glucocorticoid receptor (GR), was analyzed. The plasma FFA concentration increased 2-fold, 10 minutes (P < 0.001), 20 minutes (P < 0.01), and 60 minutes (P < 0.01) post-heparin. The corticosterone (B) and progesterone concentrations were unchanged 60 minutes post-injection. The binding activity of immature rat CBG for B dropped 50% (P < 0.001) 60 minutes post-heparin injection, decreased B binding and increased plasma FFA were correlated (r = -0.8). The decreased B binding resulted from a 2-fold decrease in the apparent number of CBG binding sites; the affinity constant (Ka) remained unchanged. The liver cytosol endogenous FFA content of immature rats was also increased 2-fold, 60 minutes after heparin-induced lipolysis. The increased cytosol FFA, with no significant change in glucocorticoid, was accompanied by a significant decrease in dexamethasone binding to liver cytosol glucocorticoid receptor. The decrease resulted from a significantly lower apparent Ka for dexamethasone and fewer receptor binding sites (n). There was a good inverse correlation between Ka (r = -0.93) and n (r = -0.90) and the increased liver cytosol FFA content. Thus the higher plasma FFA induced in vivo by lipase activation or a standard FFA mixture probably causes conformational changes in CBG and GR, reducing glucocorticoid binding to immature rat CBG and liver GR.
Haourigui
M
M
U 224 INSERM, Laboratoire de Biochimie, Faculté de Médecine Xavier Bichat, Paris, France.
Vallette
G
G
Martin
M E
ME
Sumida
C
C
Benassayag
C
C
Nunez
E A
EA
D000818:Animals; D003345:Corticosterone / Q000097:blood; D003600:Cytosol / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D005938:Glucocorticoids / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D007700:Kinetics; D008049:Lipase / Q000097:blood; D008055:Lipids / Q000097:blood; D008066:Lipolysis / Q000187:drug effects; D008099:Liver / Q000378:metabolism*; D008297:Male; D011374:Progesterone / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D014156:Transcortin / Q000378:metabolism*
D005230:Fatty Acids, Nonesterified; D005938:Glucocorticoids; D008055:Lipids; D011965:Receptors, Glucocorticoid; D011374:Progesterone; D003907:Dexamethasone; D006493:Heparin; D014156:Transcortin; D008049:Lipase; D003345:Corticosterone