title: Crystallization and preliminary X-ray analysis of phenol hydroxylase from Trichosporon cutaneum.
abstract: Recombinant phenol hydroxylase from the soil yeast Trichosporon cutaneum has been crystallized with PEG 4000 as precipitant. The crystals are monoclinic, space group P2(1) with cell dimensions a = 101.8 A, b = 153.0 A, c = 116.0 A and beta = 114.8 degrees. The crystal asymmetric unit most likely contains two dimers of phenol hydroxylase corresponding to a packing density in the crystals of 2.54 A 3/Da. The self-rotation function is consistent with the packing of two dimers in the asymmetric unit. The observed diffraction pattern extends beyond 2.8 A resolution and the crystals are well suited for structural analysis by X-ray diffraction methods.
authors.affiliation: Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: C
authors.initials: C
authors.lastname: Enroth
authors.forename: W
authors.initials: W
authors.lastname: Huang
authors.forename: S
authors.initials: S
authors.lastname: Waters
authors.forename: H
authors.initials: H
authors.lastname: Neujahr
authors.forename: Y
authors.initials: Y
authors.lastname: Lindqvist
authors.forename: G
authors.initials: G
authors.lastname: Schneider
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000302:isolation & purification*; D014250:Trichosporon / Q000201:enzymology*
chemical_list: D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase