Isolation, sequence and expression of a cDNA encoding the alpha-chain of the feline CD8.
We have cloned, sequenced and expressed a cDNA encoding the alpha-chain of feline CD8. This clone, named FT8-10, has an open reading frame with 720 nucleotides in length encoding a protein with 239 amino acid residues. Sequence analysis has revealed that the feline CD8 alpha-chain (CD8 alpha) shares significant homology with human (T8/Leu-2), bovine (BoCD8), rat (MRC OX8) and mouse (Lyt-2) CD8 alpha subunits. Cysteine residues as well as the tyrosine kinase p56lck binding site are well conserved. Besides, no putative N-linked glycosylation site was found. Interestingly, immunofluorescence analysis of COS-7 cells transfected with feline CD8 alpha expression plasmid driven by SR alpha promoter showed that the expressed feline CD8 alpha cross-reacted with an anti-human CD8 alpha monoclonal antibody OKT8, but did not react with an anti-feline monoclonal antibody, FT-2, which is thought to recognize the feline analogue of the human T8/Leu-2 and murine Lyt-2 molecules expressed on cytotoxic/suppressor T cells.
Pecoraro
M R
MR
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Kawaguchi
Y
Y
Miyazawa
T
T
Norimine
J
J
Maeda
K
K
Toyosaki
T
T
Tohya
Y
Y
Kai
C
C
Mikami
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016827:CD8 Antigens / Q000235:genetics*; D002415:Cats / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D013045:Species Specificity
D016827:CD8 Antigens; D018076:DNA, Complementary