A sensitive ELISA for 6 beta-hydroxycortisol in urine using enzyme penicillinase (beta-lactamase).
A sensitive and specific, enzyme labelled immunosorbent assay (ELISA) for 6 beta-hydroxycortisol in diluted urine using penicillinase was developed. 6 beta-Hydroxycortisol-21-hemisuccinate was conjugated with enzyme penicillinase. Antibody immobilized on a polyvinylchloride ELISA plate (Dynatech) was used for separation of bound from free ligand. The sensitivity of the assay was between 2.0-3.0 pg per well and recovery of 6 beta-hydroxycortisol from urine ranged between 85.0-108.0%. The assay is simple, rapid and precise.
Patel
S B
SB
Institute for Research in Reproduction (ICMR), Parel, Bombay, India.
Khatkhatay
I
I
Desai
M P
MP
Betrabet
S S
SS
Toddywalla
V S
VS
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000652:urine; D011143:Polyvinyl Chloride; D012680:Sensitivity and Specificity; D001618:beta-Lactamases
C001380:6 beta-hydroxycortisol; D011143:Polyvinyl Chloride; D001618:beta-Lactamases; D006854:Hydrocortisone