[Detection of hepatitis B virus DNA using local nucleic acid amplification (polymerase chain reaction) in fixed tissues in chronic viral liver diseases].
PCR is a highly sensitive, convenient and rapid method for detection of viral DNA in fixed tissue samples which allows one to analyse the material from the pathology files, mainly biopsies. PCR in a fixed material requires larger amounts of DNA-polymerase and longer duration of every stage of thermo-cycles compared to PCR in the purified DNA samples.
Tagieva
N E
NE
Lomonosov
M Iu
MIu
Ignatova
T M
TM
Aprosina
Z G
ZG
Drozdova
A S
AS
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008107:Liver Diseases / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016707:Tissue Fixation; D014777:Virus Diseases / Q000382:microbiology*
D004279:DNA, Viral
Vyiavlenie DNK virusa gepatita v metodom lokal'noĭ amplifikatsii nukleinovykh kislot (polimeraznaia tsepnaia reaktsiia) v fiksirovannykh tkaniiakh pri khronicheskikh virusnykh zabolevaniiakh pecheni.