Cloning of a recA-like gene from the diazotroph Herbaspirillum seropedicae strain Z78.
A recombinant plasmid, pBMR5, carrying a recA-like gene of Herbaspirillum seropedicae, was isolated from a H. seropedicae genomic library by intergeneric complementation of Escherichia coli recA mutant strain HB101. Quantitative survival experiments showed that pBMR5 restored the ultraviolet radiation and methyl methanesulfonate resistances and recombinational proficiency of this strain. Hybridization studies showed that there is DNA sequence homology between the recA gene of E. coli K12 and that of H. seropedicae. Restriction sites for EcoRI, HindIII, BamHI, and Bg/II were found in the DNA insert derived from H. seropedicae in pBMR5. A Tn5 insertional mutant of pBMR5, called pBMR26.2, failed to restore recombination proficiency and methyl methanesulfonate and ultraviolet resistance to recA mutants of E. coli.
Steffens
M B
MB
Department of Biochemistry, Universidade Federal do Paraná, Curitiba, Brazil.
Rigo
L U
LU
Funayama
S
S
Souza
E M
EM
Machado
H B
HB
Pedrosa
F O
FO
D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006090:Gram-Negative Bacteria / Q000235:genetics*; D016254:Mutagenesis, Insertional; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D011938:Rec A Recombinases