title: Absence of a transcellular oxalate transport mechanism in LLC-PK1 and MDCK cells cultured on porous supports.
abstract: Transepithelial oxalate transport across polarized monolayers of LLC-PK1 cells, grown on collagen-coated microporous membranes in Transwell culture chambers, was studied in double-label experiments using [14C]-oxalate together with [3H]-D-mannitol as an extracellular marker. The [14C]-labeled glucose analog alpha-methyl-glucoside (alpha-MG) was used as functional marker for active proximal tubular sugar transport. Cellular uptake of oxalate and alpha-MG at both the apical and basolateral plasma membrane was determined. When added to the upper compartment, alpha-MG was actively taken up at the apical membrane, directed through the cells to the basolateral membrane and transported to the lower compartment, indicating functional epithelial sugar transport by LLC-PK1 cells. In LLC-PK1 cells, the uptake of alpha-MG at the apical membrane was approximately 50 times higher than that at the basolateral membrane. In contrast to this active transport of sugar, LLC-PK1 cells did not demonstrate oxalate uptake either at the apical or basolateral plasma membrane. The apical-to-basolateral (A- > B) flux of oxalate in LLC-PK1 cells was identical to the basolateral-to-apical (B- > A) oxalate flux in these cells. Moreover these flux characteristics were similar to those found for D-mannitol, indicating paracellular movement for both compounds. From these data, it is concluded that, under the experimental conditions used, LLC-PK1 cells do not exhibit a specific transcellular transport system for oxalate.
authors.affiliation: Department of Urology, Erasmus University Rotterdam, The Netherlands.
authors.forename: C F
authors.initials: CF
authors.lastname: Verkoelen
authors.forename: J C
authors.initials: JC
authors.lastname: Romijn
authors.forename: W C
authors.initials: WC
authors.lastname: de Bruijn
authors.forename: E R
authors.initials: ER
authors.lastname: Boevé
authors.forename: L C
authors.initials: LC
authors.lastname: Cao
authors.forename: F H
authors.initials: FH
authors.lastname: Schröder
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002460:Cell Line; D016764:Cell Polarity / Q000502:physiology; D015194:Diffusion Chambers, Culture; D004848:Epithelium / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008353:Mannitol / Q000493:pharmacokinetics; D008757:Methylglucosides / Q000493:pharmacokinetics; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D010070:Oxalates / Q000493:pharmacokinetics*
chemical_list: D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; D010070:Oxalates; D008353:Mannitol; C027020:methylglucoside