Influence of bulky side chains of amino acids on the solution conformation of peptide fragment (81-92) derivatives of CD4, TYICEVEDQKEE, as studied by NMR spectroscopy and molecular modeling.
Solution conformation of CD4 fragment 81-92 TYICEVEDQKEE and two of its benzylated analogues was determined by two-dimensional 1H NMR spectroscopy, distance geometry and simulated annealing techniques. The structures of both benzylated derivatives are similar but are distinct from that of wild-type dodecapeptide. It is concluded from structural analysis that bulky side chain(s) of amino acid(s) at an appropriate position can have a marked effect on the conformation and thus the functions of a peptide.
Chang
D K
DK
Institute of Chemistry, Academia Sinica, Taipei, Taiwan, R.O.C.
Liang
C C
CC
D000595:Amino Acid Sequence; D001555:Benzene Derivatives / Q000737:chemistry; D015704:CD4 Antigens / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation; D012996:Solutions
D001555:Benzene Derivatives; D015704:CD4 Antigens; D009842:Oligopeptides; D010446:Peptide Fragments; D012996:Solutions; C086848:CD4 (81-92)