title: Phosphoryl transfer in Flp recombination: a template for strand transfer mechanisms.
abstract: The basic chemistry involved in DNA recombination, RNA splicing and DNA transposition is a phosphoryl transfer reaction. This review is an attempt to provoke a unified thinking on the reaction mechanisms in these nucleic acid transactions. Some of the recent results with the Flp site-specific recombinase that reveal how the chemical reactivity for recombination is derived from cooperative protein-subunit interactions on the DNA substrate are discussed. At least some of the features of Flp reaction are likely to have global implications in other DNA and RNA strand-transfer systems.
authors.affiliation: Department of Microbiology, University of Texas at Austin 78712.
authors.forename: M
authors.initials: M
authors.lastname: Jayaram
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000737:chemistry* / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology; D005656:Fungal Proteins / Q000737:chemistry; D015321:Gene Rearrangement; D008969:Molecular Sequence Data; D010710:Phosphates; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D005656:Fungal Proteins; D010710:Phosphates; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase