Cytotoxicity of sulphur mustard on a human keratinocyte cell line: direct effects compared to conditioned-medium effects.
Direct toxic effects of sulphur mustard (SM) were compared to SM-conditioned medium effects in a human keratinocyte cell line. Cytotoxicity was evaluated by measurement of cell viability with the Neutral Red uptake assay. Culture directly exposed to SM exhibited cytotoxic dose-response curves after 24, 48 or 72 h. Inhibitory concentration 50 (IC50) appeared to be more than 10 times lower after 72 h than the corresponding value after 24 h. In contrast, no cytotoxic activity was observed in media which were collected 4 h or 24 h after SM exposure. Cell viability was unchanged even if observation was extended to 48 h. Results suggested that SM cytotoxicity was not due to potential mediators secreted by human keratinocytes.
Vindevoghel
L
L
Department of Biology, Centre d'Etudes du Bouchet, Vert-Le-Petit, France.
Capdevila
C
C
Binder
P
P
Adolphe
M
M
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D017077:Culture Media, Conditioned; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D007928:Lethal Dose 50; D009151:Mustard Gas / Q000633:toxicity*; D013997:Time Factors
D017077:Culture Media, Conditioned; D009151:Mustard Gas