Morphological changes in oligodendrocytes in the intact mouse optic nerve following intravitreal injection of tumour necrosis factor.
Intracellular dye-injection was used to determine the whole-cell morphology of oligodendrocytes in intact optic nerves of mice following intravitreal injection of tumour necrosis factor-alpha (TNF alpha) or heat-inactivated TNF alpha to act as controls. Oligodendrocytes in control nerves had a stereotypic morphology, and provided an average of 20 axons with single internodal myelin segments of around 200 microns internodal length. Oligodendrocytes with abnormal morphological features were identified 8-14 days following intravitreal injection of TNF alpha. Internodal myelin segments developed swellings along their lengths, became attenuated, and in extreme cases receded completely. This study provides a new insight into the process of demyelination, especially of the early stages which are not amenable with other techniques. Furthermore, it confirms that injection of TNF alpha into the vitreous, a fluid compartment of the CNS, instigates a sequence of events which results in oligodendrocyte disruption and demyelination. The mechanism by which intravitreally injected TNF alpha mediates these changes in optic nerve oligodendrocytes are yet unknown.
Butt
A M
AM
Sherrington School of Physiology, UMDS, St. Thomas's Hospital, London, UK.
Jenkins
H G
HG
D000818:Animals; D006735:Horseradish Peroxidase; D066298:In Vitro Techniques; D007267:Injections; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects; D009900:Optic Nerve / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014822:Vitreous Body
D014409:Tumor Necrosis Factor-alpha; D006735:Horseradish Peroxidase