title: Developmentally excised sequences in micronuclear DNA of Paramecium.
abstract: DNA processing occurs in ciliates at autogamy and conjugation when new macronuclei are formed from micronuclei and old macronuclei degrade. Processing of micronuclear DNA consists of removal of certain internal sequences, chromosomal fragmentation, addition of new telomeres, and amplification. Aside from a recent brief report, internal eliminated sequences have not been described in Paramecium. In this paper we characterize nine internal eliminated sequences found within and near the gene that codes for surface protein A in Paramecium tetraurelia. Of these nine, seven are located within the translated portion of the gene, and all include short, inverted terminal repeats. The characteristic sequence, TA, appears at the boundaries of all of the internal eliminated sequences.
authors.affiliation: Department of Biology, Indiana University, Bloomington 47405-6801.
authors.forename: C J
authors.initials: CJ
authors.lastname: Steele
authors.forename: G A
authors.initials: GA
authors.lastname: Barkocy-Gallagher
authors.forename: L B
authors.initials: LB
authors.lastname: Preer
authors.forename: J R
authors.initials: JR
authors.lastname: Preer
mesh_terms: D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D016054:DNA, Protozoan / Q000378:metabolism; D048631:Micronucleus, Germline / Q000378:metabolism*; D008969:Molecular Sequence Data; D010247:Paramecium / Q000235:genetics* / Q000254:growth & development; D014176:Protein Biosynthesis; D017384:Sequence Deletion
chemical_list: D004251:DNA Transposable Elements; D016054:DNA, Protozoan