title: Photoaffinity cross-linking of F1ATPase from spinach chloroplasts by 3'-arylazido-beta-alanyl-8-azido ATP.
abstract: UV irradiation of the ATPase (CF1) from spinach chloroplasts in the presence of 3'-arylazido-beta-alanyl-8-azido ATP (8,3'-DiN3ATP) results in a nucleotide-dependent inactivation of the enzyme and in a nucleotide-dependent formation of alpha-beta cross-links. The results demonstrate an interfacial localization of the nucleotide binding sites on CF1.
authors.affiliation: Institut für Biochemie, Johannes Gutenberg-Universität, Mainz, Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Schäfer
authors.forename: G
authors.initials: G
authors.lastname: Rathgeber
authors.forename: A
authors.initials: A
authors.lastname: Schuhen
authors.forename: R J
authors.initials: RJ
authors.lastname: Berzborn
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives; D000345:Affinity Labels; D001386:Azides; D002736:Chloroplasts / Q000201:enzymology*; D003432:Cross-Linking Reagents; D010777:Photochemistry; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D014675:Vegetables
chemical_list: C026397:3'(O)-(3-(N-(4-azido-2-nitrophenyl)amino)propionyl)-8-azidoadenosine 5'-triphosphate; D000345:Affinity Labels; D001386:Azides; D003432:Cross-Linking Reagents; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases