title: Crude or recombinant proteins applied to latex agglutination, complement fixation and enzyme-linked immunosorbent assays for the serodiagnosis of invasive amebiasis.
abstract: Three different agglutination tests were developed for the detection of serum antibodies against Entamoeba histolytica. These tests are based on carboxylated polystyrene beads loaded either with a purified recombinant E. histolytica protein, designated recEh-P1, or a soluble fraction, or a membrane fraction (M-LA) both prepared from E. histolytica trophozoites. The three agglutination tests were compared with an enzyme-linked immunosorbent assay and a complement fixation test based on crude soluble E. histolytica antigens as well as with an enzyme-linked immunosorbent assay using recEh-P1 as antigen (P1-EIA). Serum samples from patients with invasive amebiasis (n = 30), or infectious diseases unrelated to E. histolytica (n = 57), as well as sera of apparently healthy individuals (n = 25) including some with noninvasive amebiasis (n = 5) were analysed by all six methods. Depending on the assay used, the results obtained, revealed sensitivities ranging from 83% to 100% and specificities ranging from 93% to 100%. P1-EIA and M-LA exhibited best results, both with a sensitivity of 100% and a specificity of 98%.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Lotter
authors.forename: E
authors.initials: E
authors.lastname: Mannweiler
authors.forename: E
authors.initials: E
authors.lastname: Tannich
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology; D003168:Complement Fixation Tests; D004748:Entamoeba histolytica / Q000737:chemistry / Q000276:immunology*; D004749:Entamoebiasis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006801:Humans; D007841:Latex Fixation Tests; D008863:Microspheres; D015800:Protozoan Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D011994:Recombinant Proteins