Single crystals of a type III antifreeze polypeptide from ocean pout.
Antifreeze protein (HPLC-2) from ocean pout was purified from serum using column chromatography on Sephadex G75 and reverse-phase HPLC columns. Single crystals were grown by batch methods at 4 degrees C from a 1.5 M solution of ammonium sulphate (pH 7.1). The crystals diffracted to about 2.5 A resolution at 4 degrees C and belong to the monoclinic space group P2(1), with cell parameters: a = 39.77 A, b = 58.51 A, c = 30.27 A, beta = 102.28 degrees, with two molecules of 6000 M(r) per asymmetric unit.
Xue
Y Q
YQ
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Sicheri
F
F
Ala
P
P
Hew
C L
CL
Yang
D S
DS
D000818:Animals; D021301:Antifreeze Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D005399:Fishes; D005615:Freezing; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification
D021301:Antifreeze Proteins; D006023:Glycoproteins