title: Purification and characterization of urotensin II from the brain of a teleost (trout, Oncorhynchus mykiss) and an elasmobranch (skate, Raja rhina).
abstract: Peptides related to urotensin II have been isolated in pure form from an extract of whole brain of a teleost, the rainbow trout (Oncorhynchus mykiss) and of an elasmobranch, the longnose skate (Raja rhina). The primary structure of the trout peptide [Gly-Gly-Asn-Ser-Glu-Cys-Phe-Trp-Lys-Tyr-Cys-Val] is similar to that of urotensin II peptides isolated from the urophyses of other teleost fish. For example, trout urotensin II contains only one amino acid substitution (Thr4-->Ser) compared with urotensin II beta 1 isolated from the urophysis of the carp. The primary structure of the skate peptide [Asn-Asn-Phe-Ser-Asp-Cys-Phe-Trp-Lys-Tyr-Cys-Val] is the same as urotensin II isolated from the caudal spinal cord region of the dogfish Scyliorhinus canicula. The data provide chemical evidence to support the conclusion of earlier morphological studies [Yulis, C. R., and Lederis, K. (1988) Gen. Comp. Endocrinol. 70, 301-311) that certain species of fish possess an extensive extraurophyseal distribution of urotensin II-immunoreactive neurons.
authors.affiliation: Department of Biomedical Sciences, Creighton University, Omaha, Nebraska 68178.
authors.forename: D
authors.initials: D
authors.lastname: Waugh
authors.forename: J M
authors.initials: JM
authors.lastname: Conlon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D012860:Skates, Fish / Q000378:metabolism*; D013045:Species Specificity; D014337:Trout / Q000378:metabolism*; D014579:Urotensins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D014579:Urotensins; C029677:urotensin II