title: [The effect of the ascorbic acid-Cu (II) couple on enzyme activity].
abstract: Ascorbic acid (AA), Cu (II) and their association affect in different ways the activity of G6P-ase and G6PDH from the rat hepatic cytoplasm enriched in microsomes. G6P-ase is inhibited at a wide range of concentrations: 200-2 mM AA and 1-0.1 mM Cu (II). The association AA-Cu in the above mentioned concentrations, proved to be a stronger inhibitor than AA or Cu (II) introduced separately in the incubation medium. The activity of G6PDH is stimulated by AA-Cu (II) association and inhibited by AA in the dialyzed and nondialyzed cytoplasm. Cu (II) inhibits the activity of G6PDH in the nondialyzed cytoplasm and stimulates it following dialysis. The compounds with recognized antiradical action prove to be inefficient in restoring the activity of G6PDH affected by the AA-Cu (II) association.
authors.affiliation: Disciplina de Biochimie, Universitatea de Medicină şi Farmacie Iaşi.
authors.forename: M
authors.initials: M
authors.lastname: Filip
authors.forename: I
authors.initials: I
authors.lastname: Păduraru
authors.forename: L
authors.initials: L
authors.lastname: Jerca
authors.forename: A
authors.initials: A
authors.lastname: Iacobovici
authors.forename: A
authors.initials: A
authors.lastname: Saramet
authors.forename: F
authors.initials: F
authors.lastname: Filip
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors / Q000187:drug effects*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000187:drug effects*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D051381:Rats
chemical_list: D003300:Copper; D005954:Glucosephosphate Dehydrogenase; D005952:Glucose-6-Phosphatase; D001205:Ascorbic Acid
vernacular_title: Efectul cuplului acid-ascorbic-Cu (II) asupra activităţii unor enzime.