Simultaneous determination of felbamate, primidone, phenobarbital, carbamazepine, two carbamazepine metabolites, phenytoin, and one phenytoin metabolite in human plasma by high-performance liquid chromatography.
An isocratic liquid-chromatographic method employing one extraction step has been developed for the quantitation of five drugs and three metabolites in human plasma. The method uses 0.100-ml aliquots of human plasma and two internal standards. Chromatographic conditions include a 4.6 mm x 150 mm Spherisorb ODS2, 3 microns a high-performance liquid chromatography, (HPLC) column, a phosphate buffer-acetonitrile-methanol (700:160:140) mobile phase, and ultraviolet (UV) absorbance detection at 210 nm. Analytes and linear quantitation ranges (microgram/ml) were felbamate (FBM) 0.391-200; primidone (PRIM), 0.098-100; phenobarbital (PHENO), 0.195-100; carbamazepine (CBZ), 0.195-100; phenytoin (PHT), 0.195-200. For CBZ-transdiol (CBZ-TR) CBZ-epoxide (CBZ-EP), and the PHT metabolite, 5-(4-hydroxyphenyl)-5-phenylhydantoin (HPPH), the range was 0.049-25.0 micrograms/ml. Ethosuximide, methsuximide, 2-methyl-2-phenyl-succinimide (methsuximide metabolite), 2-ethyl-2-phenyl malonamide (PRIM metabolite, 5-ethyl-5-(4-hydroxyphenyl)-barbituric acid (PHENO metabolite), and mephenytoin do not interfere with quantitation of the above compounds.
Romanyshyn
L A
LA
Department of Biochemistry, Wallace Laboratories, Cranbury, New Jersey 08512.
Wichmann
J K
JK
Kucharczyk
N
N
Shumaker
R C
RC
Ward
D
D
Sofia
R D
RD
D000927:Anticonvulsants / Q000097:blood*; D002220:Carbamazepine / Q000031:analogs & derivatives / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D000078328:Felbamate; D006801:Humans; D010634:Phenobarbital / Q000097:blood; D048448:Phenylcarbamates; D010672:Phenytoin / Q000031:analogs & derivatives / Q000097:blood; D011324:Primidone / Q000031:analogs & derivatives / Q000097:blood; D011409:Propylene Glycols / Q000097:blood; D011786:Quality Control; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet
D000927:Anticonvulsants; D048448:Phenylcarbamates; D011409:Propylene Glycols; D011324:Primidone; D002220:Carbamazepine; D010672:Phenytoin; D000078328:Felbamate; D010634:Phenobarbital