Expression and stability of recombinant RQ-mRNAs in cell-free translation systems.
Expression of dihydrofolate reductase (DHFR) and chloramphenicol acetyltransferase (CAT) mRNAs in cell-free Escherichia coli translation systems is greatly enhanced as a result of their insertion into RQ135 RNA, a naturally occurring satellite of phage Q beta. The enhancement is due to protection of the recombinant mRNAs against endogenous ribonucleases and to an increased initial rate of translation in the case of the RQ-CAT mRNA.
Ugarov
V I
VI
Institute of Protein Research, Russian Academy of Sciences, Puschino, Moscow Region.
Morozov IYu
Jung
G Y
GY
Chetverin
A B
AB
Spirin
A S
AS
D017908:Allolevivirus / Q000235:genetics*; D002474:Cell-Free System; D015500:Chloramphenicol O-Acetyltransferase / Q000037:antagonists & inhibitors; D003001:Cloning, Molecular; D005493:Folic Acid Antagonists; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics
D005493:Folic Acid Antagonists; C028696:Qbeta ribonucleic acid; D012333:RNA, Messenger; D012367:RNA, Viral; D015500:Chloramphenicol O-Acetyltransferase