title: Multiple DNA conformational changes induced by an initiator protein precede the nicking reaction in a rolling circle replication origin.
abstract: The core origin for plus strand DNA replication of filamentous bacteriophage f1 binds the initiator protein (gpII), which subsequently introduces a specific nick in the plus strand. The core origin consists of a nicking region and a binding region. The binding of gpII occurs in two steps, forming a binding intermediate (complex I) and a functional complex for nicking (complex II). Results of gel retardation experiments using circularly permuted DNA fragments and direct visualization by electron microscopy show that gpII induces successive bends within the binding region upon formation of the complexes. We show that gpII binding induces duplex melting in the nicking region using KMnO4 modification of unpaired thymidine residues as a probe for melting. Origin binding occurred in the absence of superhelicity of DNA and Mg2+, whereas duplex melting required superhelical DNA, but not Mg2+. Deletion analyses indicated that hypothetical formation of a cruciform around the nicking site is not necessary for either melting or nicking. A mutation in gpII resulted in stimulation of duplex melting and nicking without showing obvious effects on bending. This suggests that the mechanism of melting involves local interaction between gpII and the nicking region. Furthermore, using synthetic oligonucleotide substrates, we show that the nicking reaction takes place efficiently when the nicking region is single-stranded and the binding region is double-stranded. These results indicate that the nicking reaction is preceded by an ordered series of protein-induced DNA-conformational changes: successive bending of the origin upon gpII binding, followed by duplex melting that requires negative superhelicity.
authors.affiliation: Department of Microbial Genetics, National Institute of Genetics, Mishima, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Higashitani
authors.forename: D
authors.initials: D
authors.lastname: Greenstein
authors.forename: H
authors.initials: H
authors.lastname: Hirokawa
authors.forename: S
authors.initials: S
authors.lastname: Asano
authors.forename: K
authors.initials: K
authors.lastname: Horiuchi
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003090:Coliphages / Q000378:metabolism*; D004261:DNA Replication; D004278:DNA, Superhelical / Q000737:chemistry* / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D008961:Models, Structural; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D015183:Restriction Mapping; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: C024013:DNA replication complex protein, Bacteriophage lambda; D004278:DNA, Superhelical; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; C488880:gene II protein, Enterobacteria phage f1