Philadelphia chromosome as the sole abnormality and p210 bcr-abl chimeric protein expression in an Epstein-Barr virus-transformed B cell line from a patient with chronic myeloid leukemia.
A 'B' cell line, originating from a patient with chronic myeloid leukemia and containing the Philadelphia chromosome, was established after Epstein-Barr virus transformation. The Philadelphia chromosome was the sole chromosomal abnormality in this line, designated as PhB1 cell line. In DNA hybridization studies we detected rearrangements in the bcr gene and in the immunoglobulin heavy chain joining region. The phenotypes of the cells were typical of mature B cells expressing antigens CD19, CD20, CD22, CD23, CD39, HLADR, IgM, and kappa. The expression of the 210 bcr-abl chimeric protein was detected by means of an immunoprecipitate assay.
Knuutila
S
S
Department of Medical Genetics, University of Helsinki, Finland.
Lindlöf
M
M
Kovanen
P E
PE
Ramqvist
T
T
Ruutu
T
T
Andersson
L C
LC
D001402:B-Lymphocytes / Q000378:metabolism*; D002472:Cell Transformation, Viral / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010677:Philadelphia Chromosome; D000091402:Prohibitins; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured
C000717328:PHB protein, human; D000091402:Prohibitins; D011993:Recombinant Fusion Proteins; D004247:DNA