Crystallization of DNA polymerase II from Escherichia coli.
DNA polymerase II of Escherichia coli, an alpha-like or group B polymerase, has been crystallized. The crystals are orthorhombic, space group P2(1)2(1)2, with cell dimensions a = 94.4 A, b = 118.2 A, c = 84.2 A and diffract to at least 3.0 A resolution. This is the first example of a group B polymerase to be crystallized.
Anderson
W F
WF
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Prince
D B
DB
Yu
H
H
McEntee
K
K
Goodman
M F
MF
D003460:Crystallization; D018360:Crystallography, X-Ray; D004257:DNA Polymerase II / Q000737:chemistry / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology*
D004257:DNA Polymerase II