title: Gender determination by multiplex PCR amplification of alphoid repeat sequences from single cells.
abstract: Alphoid repeat sequences on the X and Y chromosomes were successfully and consistently coamplified to determine the sex of single cells. Buccal cells served as a convenient model for single blastomeres obtained from preimplantation embryos through in vitro fertilization. The potential use of this technique for preimplantation genetic diagnosis is discussed.
authors.affiliation: San Francisco Center for Reproductive Medicine, CA 94118.
authors.forename: N
authors.initials: N
authors.lastname: Steuerwald
authors.forename: H
authors.initials: H
authors.lastname: Lambert
authors.forename: A J
authors.initials: AJ
authors.lastname: Steinleitner
authors.forename: R J
authors.initials: RJ
authors.lastname: Herrera
mesh_terms: D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012732:Sex Determination Analysis