Characterization of surface markers present on cells infected by chicken anemia virus in experimentally infected chickens.
Specific-pathogen-free chickens were infected with chicken anemia virus (CAV) at 1 day of age and killed after 6 days. Using a double-antibody staining procedure, spleen, thymus, and bone-marrow cells containing CAV antigen were stained for presence of T-cell antigens and chicken major histocompatibility complex (MHC) Class 1 and 2 antigens. The results demonstrated CAV infection of precursor T-cells in the thymus and of mature T-lymphocytes in the spleen. A significant proportion of the cells infected in bone marrow expressed MHC Class 2 antigens but did not exhibit the characteristics of T-lymphocytes. Since CAV grows in MDCC-MSB1 cells, the staining characteristics of these cells were also studied. MSB1 cells possessed all of the characteristics of mature, helper T-lymphocytes.
Adair
B M
BM
Department of Agriculture, Veterinary Sciences Division, Stormont, Belfast, Northern Ireland.
McNeilly
F
F
McConnell
C D
CD
McNulty
M S
MS
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology / Q000382:microbiology; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002460:Cell Line; D002642:Chick Embryo; D017727:Chicken anemia virus; D002645:Chickens; D018173:Circoviridae Infections / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007150:Immunohistochemistry; D011948:Receptors, Antigen, T-Cell; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology / Q000382:microbiology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000956:Antigens, Viral; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D011948:Receptors, Antigen, T-Cell