title: Circulating cornea-specific antibodies in corneal disease and cornea transplantation.
abstract: In order to establish the significance of circulating cornea-specific antibodies, we determined the presence of anti-corneal antibodies in the serum of 100 patients with corneal disease and in 50 healthy controls, and subsequently followed the pattern of antibody reactivity in 46 patients who underwent corneal transplantation. An indirect immunofluorescence test on cryostat sections of rabbit corneas was used for screening. The reactivity against two known bovine corneal epithelial proteins was also tested: a 54-kD protein (BCP 54) and an 11-kD protein (BCP 11/24). No significant difference in the presence and specificity of anti-corneal antibodies was observed between the group of patients with corneal disease, taken as a whole, and the healthy controls. Patients with keratoconus or non-immunological graft failure, however, were significantly more often positive for anti-corneal antibodies. Neither the presence of antibodies prior to corneal transplantation nor their appearance post-transplantation had a predictive value for corneal graft survival.
authors.affiliation: Department of Ophthalmology, Leiden University Hospital, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Jager
authors.forename: A
authors.initials: A
authors.lastname: Vos
authors.forename: S
authors.initials: S
authors.lastname: Pasmans
authors.forename: R
authors.initials: R
authors.lastname: Hoekzema
authors.forename: L
authors.initials: L
authors.lastname: Broersma
authors.forename: R
authors.initials: R
authors.lastname: van der Gaag
mesh_terms: D000444:Aldehyde Dehydrogenase; D000918:Antibody Specificity; D001323:Autoantibodies / Q000096:biosynthesis; D003315:Cornea / Q000276:immunology*; D003316:Corneal Diseases / Q000276:immunology* / Q000601:surgery; D016039:Corneal Transplantation / Q000276:immunology*; D005136:Eye Proteins / Q000276:immunology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D012086:Reoperation
chemical_list: D001323:Autoantibodies; D005136:Eye Proteins; C074043:corneal protein 11-24kDa, bovine; C030863:corneal protein 54, bovine; D000444:Aldehyde Dehydrogenase