Ultrastructural analysis of a human megakaryocytic leukemia cell line (CMK11-5) following prostaglandin E1 stimulation.
We examined the ultrastructural changes occurring in a human megakaryocytic leukemia cell line, CMK11-5, in response to stimulation with prostaglandin E1 (PGE1). The addition of PGE1 to cultured cells dose-dependently caused vacuole enlargement, the development of cell surface projections, cell surface vacuole formation, and the release of a tracer protein (horseradish peroxidase) which had been incorporated into cytoplasmic granules. These morphological changes, which occurred parallel with [Ca2+]i mobilization in the CMK11-5 cells, were similar to those observed in CMK cells in response to stimulation with ADP, thrombin, and collagen. Our present findings suggest that PGE1 has a stimulatory effect on CMK11-5 cells through the induction of [Ca2+]i mobilization, and that the PGE1-induced transmembrane signaling system in megakaryocytes is completely different from that in platelets.
Nagano
T
T
First Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Kishimoto
Y
Y
Kimura
T
T
Ryo
R
R
Sato
T
T
D000527:Alprostadil / Q000096:biosynthesis*; D006801:Humans; D008854:Microscopy, Electron; D015388:Organelles / Q000648:ultrastructure; D013920:Thrombocythemia, Essential / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000527:Alprostadil