title: Increased insulin receptor number and insulin responsiveness in a chicken hepatoma cell line.
abstract: Insulin receptor number and insulin responsiveness were compared in a chicken hepatoma cell line (LMH) and in normal chicken hepatocyte (cHep) cells cultured in the same conditions. LMH cells expressed two- to threefold more insulin receptors than cHep cells, without significant changes in affinity. The tyrosine kinase activity of solubilized and lectin (lentil+wheat germ agglutinin; WGA)-purified LMH receptors was higher than that of cHep receptors. The ATP hydrolytic activity previously observed in WGA-purified receptors from chicken liver membranes was also present in WGA-purified receptors from cultured cHep cells. This unidentified membrane-associated ATPase was absent from LMH membrane-solubilized material and therefore from WGA-purified LMH insulin receptors. Finally, LMH cells incorporated at least tenfold more amino isobutyric acid than cHep cells in the absence of insulin and were more responsive to insulin. The enhanced basal amino acid transport of LMH cells was most probably the consequence of their proliferative activity. The enhanced insulin responsiveness of LMH cells can be accounted for, at least in part, by one or several of the modifications presently demonstrated in LMH cells when compared with normal cultured hepatocytes: increased insulin receptor number and tyrosine kinase activity and possibly the loss of the membrane-associated ATPase.
authors.affiliation: INRA, Station de Recherches Avicoles, Endocrinologie de la Croissance et du Métabolisme, Nouzilly, France.
authors.forename: M
authors.initials: M
authors.lastname: Taouis
authors.forename: M
authors.initials: M
authors.lastname: Derouet
authors.forename: J P
authors.initials: JP
authors.lastname: Caffin
authors.forename: J
authors.initials: J
authors.lastname: Simon
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D007328:Insulin / Q000378:metabolism*; D008099:Liver / Q000166:cytology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D010766:Phosphorylation; D011972:Receptor, Insulin / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000596:Amino Acids; D007328:Insulin; D011972:Receptor, Insulin