Photoaffinity cross-linking of F1ATPase from spinach chloroplasts by 3'-arylazido-beta-alanyl-8-azido ATP.
UV irradiation of the ATPase (CF1) from spinach chloroplasts in the presence of 3'-arylazido-beta-alanyl-8-azido ATP (8,3'-DiN3ATP) results in a nucleotide-dependent inactivation of the enzyme and in a nucleotide-dependent formation of alpha-beta cross-links. The results demonstrate an interfacial localization of the nucleotide binding sites on CF1.
Schäfer
H J
HJ
Institut für Biochemie, Johannes Gutenberg-Universität, Mainz, Germany.
Rathgeber
G
G
Schuhen
A
A
Berzborn
R J
RJ
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives; D000345:Affinity Labels; D001386:Azides; D002736:Chloroplasts / Q000201:enzymology*; D003432:Cross-Linking Reagents; D010777:Photochemistry; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D014675:Vegetables
C026397:3'(O)-(3-(N-(4-azido-2-nitrophenyl)amino)propionyl)-8-azidoadenosine 5'-triphosphate; D000345:Affinity Labels; D001386:Azides; D003432:Cross-Linking Reagents; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases