Processing of interleukin-1 beta in synovial cells freshly isolated from patients with rheumatoid arthritis.
This study examined interleukin-1 beta (IL-1 beta) production by synovial cells from patients with rheumatoid arthritis (RA). Freshly isolated, plastic-adherent, synovial cells from ten of thirteen patients with RA secreted significant levels of IL-1 bioactivity, and the cells from five patients secreted more IL-1 beta bioactivity than IL-1 alpha bioactivity. Further, there was a relatively good correlation between IL-1 beta bioactivity and IL-1 beta converting enzyme (ICE) activity, suggesting that bioactive IL-1 beta is possibly generated through the action of ICE. Analysis of metabolically-labeled IL-1 by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), however, indicated that, although these cells contained both pre IL-1 alpha and beta intracellularly, they secreted predominantly pre IL-1 beta.
Kobayashi
Y
Y
Department of Biomolecular Science, Faculty of Science, Toho University, Chiba, Japan.
Ichijo
Y
Y
Nagatake
H
H
Watanabe
N
N
Nishizawa
K
K
Goto
M
M
D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000378:metabolism* / Q000601:surgery; D001681:Biological Assay; D020170:Caspase 1; D002460:Cell Line; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D007719:Knee Joint / Q000601:surgery; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008666:Metalloendopeptidases / Q000378:metabolism; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D013583:Synovial Membrane / Q000276:immunology / Q000378:metabolism*
D007375:Interleukin-1; D020170:Caspase 1; D008666:Metalloendopeptidases