title: Effect of thyroid status on the oxidative capacity of Sertoli cells isolated from immature rat testis.
abstract: Our previous studies indicate the Sertoli cell as a target for thyroid hormone action at testis level. In the present study we evaluated the effect of thyroid hormone on Sertoli cell oxidative capacity measured by specific cytochrome oxidase (COX) activity and intracellular adenosine triphosphate (ATP) content. Sertoli cells were isolated from 21-day-old rats. Hypothyroidism, induced from the day of birth by administration of 0.025% methimazole, was characterized by a severe delay of body and testis growth and resulted in a lower COX activity (-40%, p < or = 0.01) and a lower ATP content (-35%, p < or = 0.01) by isolated Sertoli cells. Administration of triiodothyronine (10 micrograms/100 g body wt on alternate days) to hypothyroid rats improved body and testis growth and restored both COX activity and ATP content. The presence of high-affinity, low-capacity binding sites for triiodothyronine in Sertoli cell mitochondria also was demonstrated. This study, unlike that carried out on the whole testis from adult rats, demonstrates that thyroid hormone affects the energy metabolism of Sertoli cells from midpubertal rat testes.
authors.affiliation: Istituto di Fisiologia Generale, Università di Genova, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Palmero
authors.forename: P
authors.initials: P
authors.lastname: Trucchi
authors.forename: M
authors.initials: M
authors.lastname: Prati
authors.forename: E
authors.initials: E
authors.lastname: Fugassa
authors.forename: A
authors.initials: A
authors.lastname: Lanni
authors.forename: F
authors.initials: F
authors.lastname: Goglia
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D003576:Electron Transport Complex IV / Q000378:metabolism / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D007037:Hypothyroidism / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008713:Methimazole / Q000494:pharmacology; D008928:Mitochondria / Q000737:chemistry; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D013737:Testis / Q000166:cytology* / Q000378:metabolism; D013961:Thyroid Gland / Q000502:physiology*; D014284:Triiodothyronine / Q000502:physiology
chemical_list: D014284:Triiodothyronine; D008713:Methimazole; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV