Interphase AgNOR quantity is not related to DNA content in 11 established human cancer cell lines.
The relationship between interphase AgNOR quantity and DNA ploidy was evaluated in 11 cell lines derived from human tumors of different origin. Interphase AgNOR quantity was measured by morphometric image analysis and DNA content by cytofluorometry. The mean interphase AgNOR area ranged from 5.56 to 21.12 microns 2 and DNA ploidy from 2.05 to 3.30c. The interphase AgNOR value and DNA content were not significantly related (r = 0.23, P = 0.52). No significant correlation was found between DNA content and cell doubling time, whereas a strict relationship was confirmed to exist between interphase AgNOR quantity and rapidity of cell proliferation.
Derenzini
M
M
Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
Trerè
D
D
Chieco
P
P
Melchiorri
C
C
D002455:Cell Division; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007399:Interphase; D009363:Neoplasm Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D009697:Nucleolus Organizer Region / Q000378:metabolism; D011003:Ploidies; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000378:metabolism* / Q000473:pathology
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009687:Nuclear Proteins