[Activity of c-myc protein is necessary in the first 6 hours of the prereplicative period for 3T3 Swiss cells].
It was shown what microinjection of polyclonal antibodies to the myc protein specifically inhibits DNA synthesis in serum-stimulated 3T3 Swiss cells during the first 6 h of the prereplicative period. The effect depends on the concentration of antibodies. Microinjections of polyclonal antibodies against the whole protein were more effective when microinjections of antibodies against parts of the protein. Microinjections of five kinds of monoclonal antibodies and their mixture were in effective. It was also shown what induction of expression of the antisense myc sequence in 3T3 Swiss cells leads to potent inhibition of DNA synthesis during the first 6 h of the prereplicative period. Thus it is clear what the myc protein participates in the early stages of preparation to replication, i.e., transition of cells from G0 to G1.
Egorov
E E
EE
Sullivan
N F
NF
Cremisi
C
C
Lyne
L
L
D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001769:Blood; D002453:Cell Cycle; D002846:Chromatography, Affinity; D051379:Mice; D008845:Microinjections; D016271:Proto-Oncogene Proteins c-myc / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D016271:Proto-Oncogene Proteins c-myc
Aktivnost' belka c-myc neobkhodima v pervye 6 ch prokhozhdeniia prereplikativnogo perioda kletkami 3T3 Swiss.