Determination of traces of pyrethrins and piperonyl butoxide in biological material by high-performance liquid chromatography.
A liquid chromatographic method for the determination of pyrethrins and of the synergist piperonyl butoxide in human plasma after C18 solid-phase extraction is described. UV detection was found to be sensitive enough to determine concentrations far below the limit of toxicity. With respect to future investigations concerning studies in biological materials, a column-switching system for sample preparation was developed and compared with solid-phase extraction. Both methods show comparable limits of detection, but the column-switching technique has the advantage of fully automating the system.
Wintersteiger
R
R
Institute of Pharmaceutical Chemistry, Karl-Franzens-University Graz, Austria.
Ofner
B
B
Juan
H
H
Windisch
M
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D006801:Humans; D010882:Piperonyl Butoxide / Q000097:blood*; D011722:Pyrethrins / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D011722:Pyrethrins; D010882:Piperonyl Butoxide