Increase in c-erbA alpha 2 mRNA in the parvocellular region of the paraventricular nucleus of the hypothalamus following thyroidectomy in the adult male rat.
To examine thyroid hormone regulation of c-erbA alpha 2 mRNA expression in the parvo-cellular region of the paraventricular nucleus of the rat hypothalamus (pPVN), quantitative in situ hybridization was performed using 3H-labeled probe complementary to c-erbA alpha 2 mRNA. Thyroidectomy induced a significant increase in the number of silver grains overlying the cytoplasm in the pPVN relative to sham-operated controls. This effect was prevented by daily injection of thyroxine. These results indicate that hypothyroidism induced an increase in cellular c-erbA alpha 2 mRNA level in the pPVN.
Koibuchi
N
N
Department of Physiology, Dokkyo University School of Medicine, Tochigi, Japan.
Gibbs
R B
RB
Jones
K E
KE
Yamaoka
S
S
Chin
W W
WW
Pfaff
D W
DW
Suzuki
M
M
D000818:Animals; D017403:In Situ Hybridization; D008297:Male; D009857:Oncogenes / Q000502:physiology*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016622:Silver Staining; D013965:Thyroidectomy; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000378:metabolism; D014284:Triiodothyronine / Q000378:metabolism
D012333:RNA, Messenger; D014284:Triiodothyronine; D013972:Thyrotropin; D013974:Thyroxine