title: Zinc hydroxide induced respiratory burst in rat neutrophils.
abstract: The effects of zinc hydroxide on the respiratory burst and phagocytosis by rat neutrophils were examined. Zinc hydroxide induced an increase in oxygen consumption and O2- production. Electronmicroscopy showed that neutrophils engulfed zinc hydroxide particles by phagocytosis. Pertussis toxin (0.25, 0.5, 1.0 micrograms/ml) and EGTA (1, 2, 5 mM) inhibited zinc hydroxide-induced O2- production in a dose-dependent manner. The inhibitors of protein kinase C, 1-(5-isoquinolinesulfonyl)-2-methyl-piperazine and N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide inhibited zinc hydroxide-induced O2- production with IC50 values ranging between 10 microM and 25 microM. The inhibitory study using an inhibitor of myosin light chain kinase, 1-(5-iodo-naphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine, showed IC50 values ranging from 5 microM to 10 microM. These findings indicate that zinc hydroxide induces respiratory burst and phagocytosis by rat neutrophils.
authors.affiliation: Department of Public Health, Yamaguchi University School of Medicine, Ube, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ogino
authors.forename: Y
authors.initials: Y
authors.lastname: Izumi
authors.forename: H
authors.initials: H
authors.lastname: Segawa
authors.forename: Y
authors.initials: Y
authors.lastname: Takeyama
authors.forename: H
authors.initials: H
authors.lastname: Ishiyama
authors.forename: T
authors.initials: T
authors.lastname: Houbara
authors.forename: T
authors.initials: T
authors.lastname: Uda
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D006878:Hydroxides / Q000494:pharmacology*; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D009504:Neutrophils / Q000187:drug effects* / Q000473:pathology; D010101:Oxygen Consumption / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D016897:Respiratory Burst / Q000187:drug effects*; D013481:Superoxides / Q000032:analysis; D017967:Zinc Compounds / Q000494:pharmacology*
chemical_list: D006878:Hydroxides; D017967:Zinc Compounds; D013481:Superoxides; C052745:zinc hydroxide