Conjugation of benzoic acid with glycine in human liver and kidney: a study on the interindividual variability.
1. The rate of conjugation of benzoic acid with glycine was measured in the homogenates of 110 specimens of human liver and in 67 specimens of human renal cortex. 2. The assay for the formation of benzoyl glycine consisted of measuring the formation of benzoyl glycine from (14C) benzoic acid and glycine in the presence of coenzyme A and ATP. 3. In human liver, the mean (+/- SD) and coefficient of variation for the formation rate of benzoyl glycine were 254 +/- 90.5 nmol min-1 per g liver and 36%, respectively. There was a weak, but significant, negative correlation (r = -0.339, p < 0.001) between the rate of formation of benzoyl glycine and the liver donor's age. 4. In the human kidney, the rate of benzoyl glycine formation was normally distributed. The mean (+/- SD) and coefficient of variation were 321 +/- 99.3 nmol min-1 per g kidney and 31%, respectively. 5. These in vitro results are consistent with the view that the in vivo rate of conjugation of carboxylic acid with glycine varies among subjects and is normally distributed.
Temellini
A
A
Department of Biomedicine, Medical School, Pisa, Italy.
Mogavero
S
S
Giulianotti
P C
PC
Pietrabissa
A
A
Mosca
F
F
Pacifici
G M
GM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism*; D019817:Benzoic Acid; D005260:Female; D005998:Glycine / Q000378:metabolism*; D006626:Hippurates / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D001565:Benzoates; D006626:Hippurates; D019817:Benzoic Acid; C030514:hippuric acid; D005998:Glycine