title: Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes.
abstract: Of 216 fecal and intestinal samples collected from nursing or weaned diarrheic pigs in the United States and Canada, 57 were identified as group A rotavirus positive by RNA electrophoresis and silver staining. Fifty-seven and 52 rotavirus-positive samples were analyzed by hybridization with Gottfried and OSU PCR-derived gene 9 and 4 probes, respectively. Only 17 samples were identified with either homologous VP4 (P)- or VP7 (G)-coding genes or both. One rotavirus identified as G4 and P7 was similar to the previously characterized interserotype rotavirus, SB-1A. Additional hybridization analyses were performed with PCR-derived probes prepared from gene 9 cDNA of the human rotaviruses Wa (G1), DS-1 (G2), and P (G3) and of the porcine rotavirus YM (G11). Eleven of 52 samples collected and analyzed from swine in Ohio, California, and Nebraska were identified as G11. No samples with G1-, G2-, or G3-type specificities were detected among the 25 of 57 rotavirus-positive samples analyzed with human rotavirus-derived probes. Further investigations with a PCR-derived gene 4 probe prepared from porcine rotavirus YM revealed hybridization specificities similar to those of the OSU gene 4 probe.
authors.affiliation: Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
authors.forename: B I
authors.initials: BI
authors.lastname: Rosen
authors.forename: A V
authors.initials: AV
authors.lastname: Parwani
authors.forename: S
authors.initials: S
authors.lastname: Lopez
authors.forename: J
authors.initials: J
authors.lastname: Flores
authors.forename: L J
authors.initials: LJ
authors.lastname: Saif
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D002170:Canada; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D018076:DNA, Complementary / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000382:microbiology / Q000662:veterinary*; D012703:Serotyping / Q000379:methods; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014481:United States
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D018076:DNA, Complementary; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus