Role of processing of the oligosaccharide chains in the affinity of lipoprotein lipase for heparin.
The role of processing of the oligosaccharide chains in the affinity of lipoprotein lipase (LPL) for heparin was examined in 3T3-L1 adipocytes. 43% of 35S-labeled LPL subunits in tunicamycin (TUN)-treated cells did not bind to a heparin-Sepharose column and 46% was eluted with 0.6 M NaCl. 11% of LPL subunits in castanospermine (CSTP)-treated cells did not bind to the column and 38% was eluted with 0.6 M NaCl. In contrast, as in untreated cells, LPL subunits in 1-deoxymannojirimycin (dMM)-treated and swainsonine (SW)-treated cells almost all bound to the column and over 93% of the subunits bound were eluted with 1.5 M NaCl. Thus, core glycosylation and subsequent removal of the distal glucose residue from oligosaccharide chains of LPL in the endoplasmic reticulum (ER) is required for acquisition of a higher affinity for heparin.
Masuno
H
H
Department of Medical Biochemistry, School of Medicine, Ehime University, Japan.
Okuda
H
H
D017485:1-Deoxynojirimycin / Q000494:pharmacology; D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006031:Glycosylation; D006493:Heparin / Q000378:metabolism*; D007212:Indolizines / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009844:Oligosaccharides / Q000378:metabolism*; D013379:Substrate Specificity; D017026:Swainsonine / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology; D054836:Indolizidines
D017485:1-Deoxynojirimycin; D006493:Heparin; D007212:Indolizines; D008071:Lipoprotein Lipase; D009844:Oligosaccharides; D017026:Swainsonine; D014415:Tunicamycin; C037806:castanospermine; D054836:Indolizidines