Anti-acetylcholine receptor (AChR) antibodies measurement in myasthenia gravis: the use of cell line TE671 as a source of AChR antigen.
Acetylcholine receptor (AChR) from the human rhabdomyosarcoma cell line TE671 was compared with that of human ischaemic muscle AChR as a source of the antigen for the diagnosis of myasthenia gravis (MG). The sera, which were anti-TE671 cell AChR antibody-negative, all came from patients with low anti-human muscle AChR antibody titers. None of the sera that were seronegative as a result of the human muscle AChR RIA became positive with TE671 cell AChR. The overall sensitivity was 7% less using TE671 cell AChR. The lower sensitivity was observed irrespective of the clinical form of MG. It also appeared from this study that epitopes specific to the junctional isoform of human AChR are essential for the detection of low antibody titers, which accounts for this feature, since TE671 cells only express the extrajunctional isoform of AChR in the surface membrane. Accordingly, AChR from cell line TE671 cannot replace human muscle AChR in the conventional diagnostic immunoprecipitation RIA. There are, however, many other useful implications of AChR from cell line TE671.
Somnier
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Department of Neurology, National Hospital (Rigshospitalet), Copenhagen, Denmark.
D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000276:immunology; D006801:Humans; D009132:Muscles / Q000276:immunology; D009157:Myasthenia Gravis / Q000276:immunology*; D011233:Precipitin Tests; D011863:Radioimmunoassay; D011950:Receptors, Cholinergic / Q000276:immunology*; D012208:Rhabdomyosarcoma / Q000276:immunology* / Q000473:pathology; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D000906:Antibodies; D000941:Antigens; D011950:Receptors, Cholinergic