Enhancement of phorbol ester-induced production of tumor necrosis factor alpha by thalidomide.
The effect of thalidomide [racemic (DL-) form and optically pure (D- and L-) forms] on tumor necrosis factor (TNF) alpha production by human leukemia cell lines (HL-60, K562 and U937) stimulated with 12-O-tetradecanoylphorbol-13-acetate (TPA) was investigated. Though thalidomide has been regarded as a specific inhibitor of TNF-alpha production, our study indicated that all forms of thalidomide enhanced (but did not inhibit) the TPA-induced TNF-alpha production by the human leukemia cell lines investigated. The effects of thalidomide on TNF-alpha production might be cell type-specific.
Nishimura
K
K
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Hashimoto
Y
Y
Iwasaki
S
S
D002460:Cell Line; D004357:Drug Synergism; D006801:Humans; D007700:Kinetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D015473:Leukemia, Promyelocytic, Acute; D016403:Lymphoma, Large B-Cell, Diffuse; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013792:Thalidomide / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D014409:Tumor Necrosis Factor-alpha; D013792:Thalidomide; D013755:Tetradecanoylphorbol Acetate