Expression of multiple oncogenes in human esophageal carcinomas.
To study the oncogenesis of human esophageal carcinoma, the expression of a variety of oncogenes was studied in 10 esophageal carcinoma cell lines and 16 pairs of tumor and nontumor tissues removed from patients with esophageal carcinoma. Northern blot analyses using 11 different oncogene probes revealed that 5 oncogenes, i.e. c-myc, c-H-ras, c-sis, c-raf, and c-fos, were expressed. Among them, a variant c-sis mRNA transcript of 2.7 kilobase (kb) was expressed in 7 of 10 cell lines and in 9 of 16 tumor tissues. Furthermore, an overexpression and an amplification of c-myc gene was observed in some cell lines. These results suggest that multiple oncogene expression may be required for the induction, maintenance, and progression of esophageal carcinoma. The expression of a 2.7-kb transcript, of c-sis and overexpression of c-myc gene may play some role in the carcinogenesis of esophageal carcinoma.
Wong
F H
FH
Graduate Institute of Microbiology and Immunology National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Hu
C P
CP
Chiu
J H
JH
Huang
B S
BS
Chang
J P
JP
Lin
P J
PJ
Chien
K Y
KY
Chang
C
C
D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000737:chemistry / Q000235:genetics*; D016762:Genes, fos; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D009857:Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D012334:RNA, Neoplasm