Immunocytochemical detection of parathyroid hormone-related protein in vascular endothelial cells.
The expression of parathyroid hormone-related protein (PTHrP) in cultured endothelial cells derived from bovine carotid artery was immunocytochemically investigated. The cells showed positive immunoreactivity for PTHrP abundantly in cytoplasm. PTHrP-like immunoreactivity was found in all endothelial cells under investigation. Negative control prepared with non-immune rabbit serum or with the antiserum preabsorbed with PTHrP showed no significant staining, indicating the specificity for immunostaining. Addition of 10% fetal calf serum to serum-deprived endothelial cells did not significantly increase PTHrP-like immunoreactivity. These results suggest that endothelial cells express PTHrP constitutively and PTHrP may act as a new class of endothelium-derived relaxing factor.
Ishikawa
M
M
Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
Ouchi
Y
Y
Akishita
M
M
Kozaki
K
K
Toba
K
K
Namiki
A
A
Yamaguchi
T
T
Orimo
H
H
D000818:Animals; D002339:Carotid Arteries; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D007106:Immune Sera; D007150:Immunohistochemistry / Q000379:methods; D010281:Parathyroid Hormone / Q000032:analysis; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins / Q000032:analysis*; D011817:Rabbits / Q000276:immunology
D007106:Immune Sera; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins