Chemical induction of stress proteins does not induce splicing thermotolerance under conditions producing survival thermotolerance.
Cell survival during a severe heat stress can be enhanced when heat shock proteins are induced prior to the severe heat treatment. Induction can be accomplished either by heat or chemical treatments. The increase in survival at these severe elevated temperatures after pretreatment has been referred to as thermotolerance, which we now refer to as survival thermotolerance. It has also been shown previously that mild heat treatment allows splicing in cells subjected to a severe heat treatment, now referred to as splicing thermotolerance. The experiments shown here demonstrate that even though chemical induction of the heat shock proteins leads to survival thermotolerance, this same treatment does not induce splicing thermotolerance. These are the first results that demonstrate at least two distinct aspects of thermotolerance.
Corell
R A
RA
Department of Biology, Dartmouth College, Hanover, New Hampshire 03755.
Riordan
J A
JA
Gross
R H
RH
D000818:Animals; D002470:Cell Survival / Q000502:physiology*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004331:Drosophila melanogaster; D000431:Ethanol / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature / Q000009:adverse effects*; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism
D006360:Heat-Shock Proteins; D012322:RNA Precursors; D012333:RNA, Messenger; D000431:Ethanol; D003513:Cycloheximide