Oligonucleotides complementary to the alpha-sarcin domain of 28S rRNA inhibit cell-free protein synthesis.
Oligodeoxynucleotides complementary to the alpha-sarcin domain of rat 28S rRNA inhibit cell-free protein synthesis. The poly(U) translation system containing Artemia salina ribosomes was more sensitive to inhibition than the system containing rat liver ribosomes. The 21-mer, which was the most effective of the 7 oligonucleotides tested, hybridized with naked 28S rRNA. Hybridization with whole ribosomes, assayed by S1 nuclease protection, occurred only at high ionic strength or with ribosomes actively engaged in protein synthesis.
Brigotti
M
M
Department of Experimental Pathology, University of Bologna, Italy.
Lorenzetti
R
R
Denaro
M
M
Carnicelli
D
D
Montanaro
L
L
Sperti
S
S
D000818:Animals; D001156:Artemia; D001345:Autoradiography; D001483:Base Sequence; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases; D005656:Fungal Proteins / Q000737:chemistry / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011072:Poly U / Q000235:genetics; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D012339:RNA, Ribosomal, 28S; D011817:Rabbits; D051381:Rats; D012270:Ribosomes / Q000378:metabolism
D005656:Fungal Proteins; D015345:Oligonucleotide Probes; D011500:Protein Synthesis Inhibitors; D012339:RNA, Ribosomal, 28S; C014801:alpha-sarcin; D011072:Poly U; D004722:Endoribonucleases