title: Phosphorylation of acidic ribosomal proteins by ribosome-associated protein kinases of Saccharomyces cerevisiae and Schizosaccharomyces pombe.
abstract: Two proteins of 13 kDa and 38 kDa, the components of 60S ribosomal subunits, were identified as phosphorylation substrates for protein kinases tightly associated with S. cerevisiae and Schizosaccharomyces pombe ribosomes. An enzyme with properties of multifunctional casein kinase II was detected in ribosome preparations from both yeast species. In S. cerevisiae another protein kinase with high substrate specificity toward those proteins was also identified. By using isoelectric focusing, the protein band of 13 kDa from S. cerevisiae and S. pombe was resolved respectively into three and four major forms of different charge. The same protein forms were phosphorylated in the in vivo 32P-labelling experiments.
authors.affiliation: Department of Molecular Biology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Jakubowicz
authors.forename: M
authors.initials: M
authors.lastname: Cytryńska
authors.forename: W
authors.initials: W
authors.lastname: Kowalczyk
authors.forename: E
authors.initials: E
authors.lastname: Gasior
mesh_terms: D000595:Amino Acid Sequence; D047390:Casein Kinase II; D047388:Casein Kinases; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins
chemical_list: D005656:Fungal Proteins; D012269:Ribosomal Proteins; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; D047390:Casein Kinase II; D047388:Casein Kinases; C479661:casein kinase II, S pombe