Gene targeting in normal somatic cells: inactivation of the interferon-gamma receptor in myoblasts.
Gene targeting in somatic cells represents a potentially powerful method for gene therapy, yet with the exception of pluripotent mouse embryonic stem (ES) cells, homologous recombination has not been reported for a well characterized, non-transformed mammalian cell. Applying a highly efficient strategy for targeting an integral membrane protein--the interferon gamma receptor--in ES cells, we have used homologous recombination to target a non-transformed somatic cell, the mouse myoblast, and to compare targeting efficiencies in these two cell types. Gene-targeted myoblasts display the properties of normal cells including normal morphology, ability to differentiate in vitro, stable diploid karyotype, inability to form colonies in soft agar and lack of tumorigenicity in nude mice.
Arbonés
M L
ML
Cell Genesys Inc., Foster City, California 94404.
Austin
H A
HA
Capon
D J
DJ
Greenburg
G
G
D000818:Animals; D001483:Base Sequence; D005821:Genetic Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D008285:Major Histocompatibility Complex; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000276:immunology*; D017471:Receptors, Interferon / Q000037:antagonists & inhibitors / Q000235:genetics*; D011995:Recombination, Genetic; D013234:Stem Cells / Q000166:cytology / Q000276:immunology; D000096743:Interferon gamma Receptor
D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor