title: A soluble neurite outgrowth molecule in bovine brain.
abstract: A neurite outgrowth molecule was purified from soluble fraction of bovine brain by reversed-phase column HPLC following concanavalin A (Con A)-affinity chromatography. This molecule was a 74kDa (named sGP74) and clearly reacted with the monoclonal antibody HNK-1. The amino acid sequences of N-terminal portion and peptides derived from trypsin digests of sGP74 were nearly identical to those of rat brain ankyrin-binding protein (ABGP186) that is a member of immunoglobulin superfamily with adhesive function. Our results suggest that sGP74 preserves multiple immunoglobulin-like domains and is released from an extracellular site of ABGP186.
authors.affiliation: Division of Biochemistry, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ishioka
authors.forename: J
authors.initials: J
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Kurioka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D002352:Carrier Proteins / Q000737:chemistry; D002417:Cattle; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry; D002460:Cell Line; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D015816:Cell Adhesion Molecules, Neuronal; D008562:Membrane Glycoproteins