title: Experimental infections of Anopheles gambiae with Plasmodium falciparum of naturally infected gametocyte carriers in Cameroon: factors influencing the infectivity to mosquitoes.
abstract: Factors which could influence the success of experimental infections of Anopheles gambiae with Plasmodium falciparum were investigated in Cameroon. 139 experimental infections with different gametocyte carriers were performed. 86 (62%) gave rise to mosquito infection after dissection of at least 20 mosquitoes. Among succeeding infections, the mean percentage of infected mosquitoes was 18.6% and mean oocyst load per positive midgut was 2.56. Only gametocyte density was identified as a factor which determined the success and the level of mosquito infection. No significant influence was found for sex and age of the gametocyte carrier, body-temperature, presence of asexual erythrocyte stages, rhesus factor, blood group and use of antimalarial drugs (chloroquine and amodiaquine).
authors.affiliation: Medical Entomology Department, ORSTOM/O.C.E.A.C., Yaounde, Cameroon.
authors.forename: T
authors.initials: T
authors.lastname: Tchuinkam
authors.forename: B
authors.initials: B
authors.lastname: Mulder
authors.forename: K
authors.initials: K
authors.lastname: Dechering
authors.forename: H
authors.initials: H
authors.lastname: Stoffels
authors.forename: J P
authors.initials: JP
authors.lastname: Verhave
authors.forename: M
authors.initials: M
authors.lastname: Cot
authors.forename: P
authors.initials: P
authors.lastname: Carnevale
authors.forename: J H
authors.initials: JH
authors.lastname: Meuwissen
authors.forename: V
authors.initials: V
authors.lastname: Robert
mesh_terms: D000017:ABO Blood-Group System; D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000655:Amodiaquine / Q000627:therapeutic use; D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D001241:Aspirin / Q000627:therapeutic use; D001831:Body Temperature; D002163:Cameroon; D002353:Carrier State / Q000188:drug therapy / Q000469:parasitology*; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D002738:Chloroquine / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology*; D016778:Malaria, Falciparum / Q000188:drug therapy / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000502:physiology*; D012044:Regression Analysis; D012737:Sex Factors
chemical_list: D000017:ABO Blood-Group System; D000655:Amodiaquine; D002738:Chloroquine; D001241:Aspirin