Photophysical alterations of erythrocyte membrane by 4,5',8-trimethyl psoralen: membrane response towards psoralen phototherapy.
Despite the wide and versatile use of psoralen phototherapy in various dermal diseases including psoriasis and leukoderma, the proposed mechanism of action of psoralen is disputed. This study launches an original documentation of free-radical resulting in erythrocyte membrane damage studied by spin-labeling using the probes malnet and 5 doxyl stearate as protein and lipid domains, respectively. Both Type I and Type II photodynamics appear plausible and the therapeutic aspects of this innovation are being verified.
Nivsarkar
M
M
SIR Hurrkisondas Nurrotumdas Medical Research Society, Bombay, India.
Kumar
G P
GP
Laloraya
M
M
D000818:Animals; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000528:radiation effects; D005033:Ethylmaleimide; D005609:Free Radicals; D006801:Humans; D008297:Male; D008563:Membrane Lipids / Q000097:blood; D008565:Membrane Proteins / Q000097:blood; D008730:Methoxsalen / Q000494:pharmacology*; D010789:Phototherapy; D011565:Psoriasis / Q000188:drug therapy; D051381:Rats; D012871:Skin Diseases / Q000188:drug therapy; D013113:Spin Labels; D013481:Superoxides / Q000097:blood; D014466:Ultraviolet Rays
D003497:Cyclic N-Oxides; D005609:Free Radicals; D008563:Membrane Lipids; D008565:Membrane Proteins; D013113:Spin Labels; D013481:Superoxides; C005072:5-doxylstearic acid; D005033:Ethylmaleimide; D008730:Methoxsalen