Specific receptors for dengue virus-induced suppressor cytokine on macrophages and lymphocytes.
The present study was undertaken to investigate the presence of receptors for the dengue virus-induced suppressor cytokine (SF) on mouse macrophages (Mphi), T and B lymphocytes. It was observed that 125I-SF bound to T and B cells to a variable degree through both high and low affinity receptors. The numbers of high affinity receptors on T and B cells were 35,000 and 16,000/cell respectively and those of low affinity receptors were 0.72 x 10(6) and 0.33 x 10(6)/cell respectively. The binding of 125I-SF was also shown by removal of the biological activity following adsorption on Mphi monolayers. The suppressor activity was inhibited by pretreatment of SF with anti-SF-antisera. The binding of 125I-SF to Mphi was saturable, reversible and dependent on pH, time and temperature. This shows the presence of a specific receptor for SF on mouse Mphi, T and B lymphocytes.
Mukherjee
R
R
Postgraduate Department of Microbiology, K. G. Medical College, Lucknow, India.
Chaturvedi
P
P
Chaturvedi
U C
UC
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D002478:Cells, Cultured; D003715:Dengue / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D006863:Hydrogen-Ion Concentration; D007108:Immune Tolerance / Q000276:immunology; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018121:Receptors, Cytokine / Q000032:analysis*; D013491:Suppressor Factors, Immunologic / Q000276:immunology / Q000378:metabolism*; D013696:Temperature
D018121:Receptors, Cytokine; D013491:Suppressor Factors, Immunologic