Structure and expression of two plant genes encoding chromoplast-specific proteins: occurrence of partially spliced transcripts.
The genes encoding fibrillin and capsanthin-capsorubin synthase are specifically expressed during fruit ripening in Capsicum annuum, leading to the accumulation of these two proteins in chromoplasts. Here, we report for the first time the cloning of genomic DNA fragments encoding these two enzymes, as well as DNA fragments containing upstream regions which are potentially involved in the regulation of the expression of these genes. While the capsanthin-capsorubin synthase gene is uninterrupted, the fibrillin gene is interrupted by two introns, the first one being inefficiently spliced. Occurrence of unspliced transcripts is apparently not related to a post-transcriptional mechanism controlling the synthesis of fibrillin or an alternative polypeptide. This work provides tools for studies on gene activation and intron splicing in plants.
Deruère
J
J
Institut de Biologie Moléculaire des Plantes du C.N.R.S., Strasbourg, France.
Bouvier
F
F
Steppuhn
J
J
Klein
A
A
Camara
B
B
Kuntz
M
M
D000595:Amino Acid Sequence; D002212:Capsicum / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D000071837:Fibrillins; D015870:Gene Expression; D017343:Genes, Plant; D007438:Introns; D008840:Microfilament Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D010940:Plant Proteins; D010946:Plants, Medicinal; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
D000071837:Fibrillins; D008840:Microfilament Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D010088:Oxidoreductases; C086535:CCS protein, Capsicum annuum