Modulation of hematopoiesis by lymphocyte membrane-derived components.
Membrane bound erythroid burst-promoting activity (mBPA) is an integral membrane protein that is present on normal B-cells and some activated T-cells, that induces burst-forming units-erythroid (BFU-E) when cultured with human erythropoietin (rHuEpo). Plasma membranes and vesicles shed from the leukemic A-1 cell line express mBPA. This activity derived from both A-1 cells and normal B-cells can be immunoadsorbed by the D3A4 antibody raised against mBPA. In this study, we demonstrate that interferon-gamma (IFN-gamma) suppresses BFU-E proliferation when added directly to culture of normal human bone marrow cells and in the absence and presence of A-1 cells. However, FACS analysis reveals that IFN-gamma enhances the surface expression of mBPA on A-1 cells. The role of IFN-gamma in modulating erythropoiesis in vitro is discussed with respect to the role of shedding membrane-derived vesicles from the B-cell surface.
Guha
A
A
Department of Medicine, University of Connecticut Health Center, Farmington 06030.
Mason
R P
RP
Chen
L
L
Tuck
D P
DP
Dainiak
N
N
D002478:Cells, Cultured; D002648:Child; D015672:Erythroid Precursor Cells / Q000187:drug effects; D004920:Erythropoiesis; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008214:Lymphocytes / Q000502:physiology*; D008222:Lymphokines / Q000032:analysis / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology*; D019714:Tissue Inhibitor of Metalloproteinases
D008222:Lymphokines; D008565:Membrane Proteins; D019714:Tissue Inhibitor of Metalloproteinases; D007371:Interferon-gamma