title: Fucoxanthin-chlorophyll a/c light-harvesting complexes of Laminaria saccharina: partial amino acid sequences and arrangement in thylakoid membranes.
abstract: The N-terminus of the major polypeptide component of the light-harvesting complex (LHC) from the brown alga Laminaria saccharina is blocked. Two partial sequences, one near the N-terminus and the other near the C-terminus, have been obtained by chemical cleavage with acetic acid and N-chlorosuccinimide. Four peptides were separated after trypsin digestion of the thylakoid membranes. One fragment is not phosphorylated, is not blocked, and has been sequenced. Purification on a reversed-phase column showed two forms of the LHC protein: the more hydrophobic form appears to be bound to photosystem I. These results are compared with LHC from other Chromophytes and the CAB family of green plants.
authors.affiliation: Laboratoire Biomembranes Végétales, CNRS URA 1810, Ecole Normale Supérieure, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Douady
authors.forename: B
authors.initials: B
authors.lastname: Rousseau
authors.forename: L
authors.initials: L
authors.lastname: Caron
mesh_terms: D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000378:metabolism; D002338:Carotenoids / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002734:Chlorophyll / Q000737:chemistry* / Q000378:metabolism; D000077194:Chlorophyll A; D002736:Chloroplasts / Q000737:chemistry / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000737:chemistry; D007795:Laminaria / Q000737:chemistry* / Q000648:ultrastructure; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000378:metabolism; D045331:Photosystem I Protein Complex; D011392:Proline / Q000378:metabolism; D017421:Sequence Analysis; D013388:Succinimides; D014357:Trypsin / Q000378:metabolism; D024341:Xanthophylls
chemical_list: D045342:Light-Harvesting Protein Complexes; D010446:Peptide Fragments; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D013388:Succinimides; D024341:Xanthophylls; C025164:fucoxanthin; C015752:N-chlorosuccinimide; D002734:Chlorophyll; C064041:chlorophyll c; D001224:Aspartic Acid; D002338:Carotenoids; D011392:Proline; D014357:Trypsin; D000077194:Chlorophyll A