A rapid and efficient, nonradioactive method for screening recombinant DNA libraries.
In this report we present a rapid and inexpensive PCR-based method to screen recombinant DNA libraries. The efficiency of this method was demonstrated by the isolation of clones of interest from three different libraries using different vector systems. This method is nonradioactive and makes it easier to handle a large number of samples since there is no need for DNA extraction. The advantages and applications of the method are discussed.
Amaravadi
L
L
Indiana University School of Medicine, Biomedical Research Institute, Terre Haute 47809.
King
M W
MW
D000818:Animals; D004274:DNA, Recombinant; D015723:Gene Library; D016133:Polymerase Chain Reaction / Q000379:methods*; D014981:Xenopus
D004274:DNA, Recombinant