Detection of cytomegalovirus in formalin-fixed paraffin-embedded donor, native and allograft liver tissue using a multiplex polymerase chain reaction-liquid hybridization assay.
A multiplex polymerase chain reaction (PCR)-liquid hybridization assay detecting a minimum 10 fg of cytomegaloviral (CMV) DNA has been developed. This assay successfully detected viral nucleic acids in 25/26 (96.2%) routinely embedded allograft liver biopsies with CMV hepatitis. No CMV DNA was detected in 15 native and 12 donor liver tissues sampled at the time of liver transplantation. These results indicate that archived formalin-fixed, paraffin-embedded liver tissues obtained from patients with significant liver disease can be successfully analyzed for CMV by PCR, retrospectively. Failure to detect CMV in native and donor livers implies that any latent virus present within these tissues is very focally distributed, or is below the threshold of detection applicable to PCR.
Randhawa
P S
PS
Department of Pathology, Presbyterian University Hospital, Pittsburgh, Pennsylvania.
Jaffe
R
R
Faruki
H
H
Rinaldo
C R
CR
Ho
M
M
Demetris
A J
AJ
Manez
R
R
Kusne
S
S
Ehrlich
G D
GD
D001483:Base Sequence; D003587:Cytomegalovirus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000382:microbiology*; D016031:Liver Transplantation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012189:Retrospective Studies
D004279:DNA, Viral