[Effect of different solutions of preservatives on the endothelial function of coronary arteries in rats].
The effects of cold storage and type of preservation solution on coronary endothelial function are not well established. Experiments were designed to evaluate coronary endothelial-dependent relaxation after a 4-hour cold (4 degrees C) storage in different preservation solutions. Rat hearts, mounted in the Langendorff apparatus, were arrested with a 10-minute perfusion of 4 degrees C crystalloid hyperkaliemic cardioplegic solution (CHCS) (KCl 24 mEq/l) and stored for 4 hours in the following preservation solutions: CHCS (n = 6), Krebs-Ringer solution (KR) (n = 6), 0.9% NaCl (NS) (n = 6) and the University of Wisconsin solution (UW) (n = 6). A fifth group (n = 6) was perfused and stored in UW solution. Endothelium-dependent and independent coronary artery vasorelaxations were respectively tested by infusing 5-hydroxytryptamine (5-HT) (10(-6) mol/l) and sodium nitroprusside (SNP) (10(-5) mol/l) before and after the storage period. In hearts stored with CHCS or KR, coronary artery flow increase to 5-HT and SNP infusions were not significantly affected. However, in hearts preserved with NS or UW solutions, 5-HT coronary response was significantly decreased, indicating endothelial dysfunction. In addition to these findings, coronary flow increase to SNP infusion was decreased in the group perfused and stored with UW, suggesting smooth muscle dysfunction. These experiments suggest that 4-hour cold storage in NS or UW impairs endothelial-dependent coronary relaxation in the isolated rat heart model.
Dagenais
F
F
Département de Chirurgie, Institut de Cardiologie de Montréal, Québec, Canada.
Cartier
R
R
Hollmann
C
C
Buluran
J
J
Carrier
M
M
Pelletier
L C
LC
D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D003331:Coronary Vessels / Q000187:drug effects; D004730:Endothelium, Vascular / Q000187:drug effects*; D005978:Glutathione / Q000494:pharmacology; D016027:Heart Transplantation / Q000379:methods; D007328:Insulin / Q000494:pharmacology; D007552:Isotonic Solutions / Q000494:pharmacology*; D008297:Male; D019188:Organ Preservation Solutions; D017680:Potassium Compounds / Q000494:pharmacology*; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D014021:Tissue Preservation / Q000379:methods*; D014664:Vasodilation / Q000187:drug effects
D007328:Insulin; D007552:Isotonic Solutions; C051865:Krebs-Ringer solution; D019188:Organ Preservation Solutions; D017680:Potassium Compounds; D012462:Saline Solution, Hypertonic; C055206:University of Wisconsin-lactobionate solution; C018369:potassium cardioplegic solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose
L'influence de différentes solutions de préservation sur la fonction endothéliale des artères coronaires du rat.