title: A novel actin cDNA is expressed in the neurons of Aplysia californica.
abstract: A novel actin cDNA has been isolated from an abdominal ganglion cDNA library of Aplysia californica by differential screening. This cDNA of 1596 nucleotides in length encodes a putative actin protein of 41.8 kDa. This protein shows 95.2% identity with another Aplysia actin gene previously shown to be expressed in the muscular sheath of the ganglion (DesGroseillers et al. (1990) Nucleic Acids Res. 18, 3654). However, the 5' and 3' untranslated regions of these cDNAs are completely different. PCR experiments performed with mRNA isolated from dissected neurons, ovotestis or kidney reveal that the gene is expressed in the neurons of the ganglia and in other tissues as well. Southern blot analysis reveals that the neuronal actin gene is a member of a large gene family.
authors.affiliation: Department of Biochemistry, University of Montreal, Canada.
authors.forename: L
authors.initials: L
authors.lastname: DesGroseillers
authors.forename: D
authors.initials: D
authors.lastname: Auclair
authors.forename: L
authors.initials: L
authors.lastname: Wickham
authors.forename: M
authors.initials: M
authors.lastname: Maalouf
mesh_terms: D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary / Q000302:isolation & purification*; D015870:Gene Expression; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*
chemical_list: D000199:Actins; D018076:DNA, Complementary