title: A protease activation mutant, MVCES1, as a safe and potent live vaccine derived from currently prevailing Sendai virus.
abstract: Sendai virus fresh isolates were shown to be antigenically different from the prototype Fushimi strain that had long been passaged in embryonated chicken eggs. Phylogenetic analysis of the hemagglutinin-neuraminidase genes also revealed the difference between these two virus groups. Both trypsin-resistant and elastase-sensitive mutations were additionally introduced to an LLC-MK2-cell-adapted and attenuated mutant derived from one of the fresh isolates. This protease activation mutant (MVCES1) showed the same antigenicity as the fresh isolates, and as a result of a single cycle of growth in lungs, it could confer better protection on mice against challenge infection with the currently prevailing Sendai virus than TR-5, which is a trypsin-resistant mutant derived from the Fushimi strain. The eligibility of MVCES1 as an attenuated live vaccine of Sendai virus is discussed.
authors.affiliation: Department of Microbiology, Kobe University School of Medicine, Japan.
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Itoh
authors.forename: H
authors.initials: H
authors.lastname: Hotta
authors.forename: M
authors.initials: M
authors.lastname: Homma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D010450:Endopeptidases / Q000494:pharmacology; D006389:Hemagglutinins, Viral / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009154:Mutation; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D018184:Paramyxoviridae Infections / Q000517:prevention & control*; D012449:Safety; D017386:Sequence Homology, Amino Acid; D014611:Vaccination; D014613:Vaccines, Attenuated; D014760:Viral Fusion Proteins / Q000187:drug effects / Q000235:genetics; D014765:Viral Vaccines
chemical_list: D006389:Hemagglutinins, Viral; D014613:Vaccines, Attenuated; D014760:Viral Fusion Proteins; D014765:Viral Vaccines; D010450:Endopeptidases