title: Microcrystals of the beta 1 isoenzyme of protein kinase C: an electron microscopic study.
abstract: Microcrystals of protein kinase C beta 1 have been grown from solutions of poly(ethylene glycol). Image analysis of electron micrographs of the protein crystals, which diffracted to 5.0 nm, revealed p3 symmetry with a unit cell of about 10.3 nm x 10.3 nm. The electron stain-excluding densities showed a three-domain ring structure in projection, giving kidney bean-shaped molecules of about 7.0 nm x 4.5 nm diameter, packed as trimers. The implications of these observations for the function of the enzyme are discussed.
authors.affiliation: Laboratory of Protein Structure, Imperial Cancer Research Fund, London, U.K.
authors.forename: R H
authors.initials: RH
authors.lastname: Newman
authors.forename: E
authors.initials: E
authors.lastname: Carpenter
authors.forename: P S
authors.initials: PS
authors.lastname: Freemont
authors.forename: T L
authors.initials: TL
authors.lastname: Blundell
authors.forename: P J
authors.initials: PJ
authors.lastname: Parker
mesh_terms: D003461:Crystallography; D007527:Isoenzymes / Q000737:chemistry / Q000648:ultrastructure*; D008854:Microscopy, Electron; D011493:Protein Kinase C / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C