In situ hybridization of mRNA for the gastric H+,K(+)-ATPase in rat oxyntic mucosa.
The H+,K(+)-ATPase member of the phosphorylating ion motive ATPases is composed of two subunits, a large alpha-subunit composed of about 1030 amino acids and a smaller beta-subunit consisting of about 290 amino acids. By biochemical and immunological methods both subunits have been found in high abundance in the gastric parietal cell. In the present study in situ hybridization was used for localizing and comparing concentrations of the mRNA for the two subunits in the gastric epithelium. For this purpose 3H-labelled probes were preferred. Hybridization was detected only in the parietal cells. The older parietal cells in the bottom of the mucosa gave a weaker hybridization signal than the younger parietal cells closer to the surface. The margin of experimental ulcers, where the parietal cells are of low differentiation, showed very weak, if any, hybridization. The differences observed in hybridization densities may reflect differences in mRNA synthesis or stability. It is conceivable that older parietal cells, as well as parietal cells of low differentiation, produce relatively small amounts of H+,K(+)-ATPase.
Bamberg
K
K
Astra Hässle AB, Mölndal, Sweden.
Nylander
S
S
Helander
K G
KG
Lundberg
L G
LG
Sachs
G
G
Helander
H F
HF
D000818:Animals; D005753:Gastric Mucosa / Q000201:enzymology*; D015870:Gene Expression; D017506:H(+)-K(+)-Exchanging ATPase / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D010295:Parietal Cells, Gastric / Q000201:enzymology; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats
D015347:RNA Probes; D012333:RNA, Messenger; D017506:H(+)-K(+)-Exchanging ATPase