Bombesin stimulates transplasma-membrane electron transport by Swiss 3T3 cells.
Bombesin, a mitogenic neuropeptide, stimulates transplasmalemma reduction of diferric transferrin or ferricyanide by Swiss 3T3 cells. The stimulation of diferric transferrin reduction occurs in the range of bombesin concentrations that stimulate proliferation of Swiss 3T3 cells. Diferric transferrin reduction by the 3T3 cells is accompanied by increased proton release from the cells and bombesin increases the differic transferrin-stimulated proton release twofold. Insulin increases the diferric transferrin reductase response and increases growth stimulation with bombesin. The effect of bombesin on the transmembrane electron transport is a new aspect of its effect on the plasma membrane in addition to increase in phosphatidylinositol turnover and protein kinase c activation. The electron transport can provide an independent mechanism of activation of the Na+/H+ exchange or it can change the redox state of pyridine nucleotide in the cytoplasm.
Sun
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Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392.
Crane
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Löw
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D016475:3T3 Cells; D000818:Animals; D001059:Apoproteins / Q000494:pharmacology; D001839:Bombesin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D004579:Electron Transport / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D051379:Mice; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D014168:Transferrin / Q000494:pharmacology
D001059:Apoproteins; D014168:Transferrin; C028284:apotransferrin; D009247:NADH, NADPH Oxidoreductases; C050129:diferric transferrin reductase; D001839:Bombesin