title: 1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice.
abstract: Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the intestinal capacity to hydrolyze lactose. We here demonstrate that the stretch -17 to -994 in the pig LPH-promoter carries cis-elements which direct a small intestinal-specific expression and a post-weaning decline of a linked rabbit beta-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-regulation.
authors.affiliation: Department of Medical Biochemistry and Genetics, Panum Institute, University of Copenhagen, Denmark.
authors.forename: J T
authors.initials: JT
authors.lastname: Troelsen
authors.forename: A
authors.initials: A
authors.lastname: Mehlum
authors.forename: J
authors.initials: J
authors.lastname: Olsen
authors.forename: N
authors.initials: N
authors.lastname: Spodsberg
authors.forename: G H
authors.initials: GH
authors.lastname: Hansen
authors.forename: H
authors.initials: H
authors.lastname: Prydz
authors.forename: O
authors.initials: O
authors.lastname: Norén
authors.forename: H
authors.initials: H
authors.lastname: Sjöström
mesh_terms: D000367:Age Factors; D000818:Animals; D005786:Gene Expression Regulation; D007421:Intestine, Small / Q000201:enzymology*; D010694:Lactase-Phlorizin Hydrolase / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D014018:Tissue Distribution; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D010694:Lactase-Phlorizin Hydrolase