title: Recombination in adaptive mutation.
abstract: The genetic requirements for adaptive mutation in Escherichia coli parallel those for homologous recombination in the RecBCD pathway. Recombination-deficient recA and recB null mutant strains are deficient in adaptive reversion. A hyper-recombinagenic recD strain is hypermutable, and its hypermutation depends on functional recA and recB genes. Genes of subsidiary recombination systems are not required. These results indicate that the molecular mechanism by which adaptive mutation occurs includes recombination. No such association is seen for spontaneous mutation in growing cells.
authors.affiliation: Department of Genetics, University of Alberta, Edmonton, Canada.
authors.forename: R S
authors.initials: RS
authors.lastname: Harris
authors.forename: S
authors.initials: S
authors.lastname: Longerich
authors.forename: S M
authors.initials: SM
authors.lastname: Rosenberg
mesh_terms: D004249:DNA Damage; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics; D016368:Frameshift Mutation; D005798:Genes, Bacterial; D007763:Lac Operon; D008957:Models, Genetic; D016296:Mutagenesis; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic; D013698:Templates, Genetic
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D011938:Rec A Recombinases; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli