A study of the genotoxic potential of flavonoids using short-term bacterial assays.
Genotoxic activities of flavonoids (quercetin, rhamnetin, isorhamnetin, apigenin, luteolin) were investigated using two short-term bacterial assays. In the "repair test" in Salmonella typhimurium (strains TA1538 uvrB- and TA1978 uvrB+) the flavonoids studied did not introduce any damage into the DNA recognized by UvrABC nuclease (correndonuclease II). The results of the SOS-Chromotest in Escherichia coli K-12 strains PQ37 (tag+, alk+) and PQ243 (tagA, alkA) indicated that flavonoids only weakly induced the SOS system. The addition of a liver activation system (S9 mix) did not increase the mutagenic effect of the flavonoids tested. Two compounds: rhamnetin, isorhamnetin and their putative metabolites formed in the presence of the S9 mix did not alkylate DNA at N-3 of adenine.
Czeczot
H
H
Department of Biochemistry, Medical School, Warsaw, Poland.
Kusztelak
J
J
D000818:Animals; D020843:Chamomile; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005419:Flavonoids / Q000378:metabolism / Q000633:toxicity*; D044948:Flavonols; D066298:In Vitro Techniques; D047311:Luteolin; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D009822:Oils, Volatile / Q000633:toxicity; D010946:Plants, Medicinal; D011794:Quercetin / Q000031:analogs & derivatives / Q000633:toxicity; D051381:Rats; D013014:SOS Response, Genetics / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D005419:Flavonoids; D044948:Flavonols; D009153:Mutagens; D009822:Oils, Volatile; C047368:3-methylquercetin; C063423:rhamnetin; D011794:Quercetin; D047311:Luteolin