cDNA cloning, expression and primary structure of Par jI, a major allergen of Parietaria judaica pollen.
A 659 bp cDNA clone** coding for an allergen of Pj pollen has been isolated from a lambda gt11 library, and its DNA sequence determined. The cDNA insert showed an open reading frame of 429 bp coding for an allergenic protein of 14,866 Da and a deduced amino acid sequence containing 143 residues. The expressed recombinant protein represented the major allergen Par jI since it reacted with 95% of the sera from Pj-allergic patients (n = 22) and with two Par jI-specific monoclonal antibodies. No similarity with other known DNA and protein sequences has been detected.
Costa
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Istituto di Biologia dello Sviluppo, CNR, Palermo, Italy.
Colombo
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P
Izzo
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Kennedy
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H
Venturella
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Cocchiara
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R
Mistrello
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G
Falagiani
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P
Geraci
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D
D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D010940:Plant Proteins; D011058:Pollen / Q000737:chemistry / Q000276:immunology*
D000485:Allergens; D018076:DNA, Complementary; D006023:Glycoproteins; C083860:Par j I protein, Parietaria judaica; D010940:Plant Proteins