Morin: a wood pigment that protects three types of human cells in the cardiovascular system against oxyradical damage.
Morin is a yellowish pigment extractable from the wood of Chlorophora tinctoria. In the present study, we have determined that morin protects three types of human cells--ventricular myocytes, saphenous vein endothelial cells, and erythrocytes--against damage by oxyradicals generated in situ. In myocytes and endothelial cells, morin prolonged substantially and in a concentration-dependent manner the survival of cells exposed to either xanthine oxidase-generated oxyradicals or superoxide radicals produced with menadione. Morin protected erythrocytes from lytic attack by peroxyl radicals generated with 2,2'-azo-bis (2-amidinopropane) dihydrochloride. In all three types of human cells, the protective effect of morin clearly excelled that displayed by Trolox (a vitamin E analog), ascorbate, or mannitol, which are water-soluble antioxidants of similar molecular size. Chemically, we verified that morin behaves as an antioxidant by diminishing markedly the amount of malondialdehyde (lipid peroxidation product) found in human cardiocytes despite their exposure to oxyradicals. In agreement with related reports, we also observed that morin is non-toxic in rats even when used at concentrations 2-3 orders of magnitude higher than those in our in vitro studies. Thus, morin acts as a broad-spectrum and non-toxic antioxidant.
Wu
T W
TW
Department of Clinical Biochemistry, University of Toronto, Toronto General Hospital, Ontario, Canada.
Zeng
L H
LH
Wu
J
J
Fung
K P
KP
D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D016166:Free Radical Scavengers; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D017382:Reactive Oxygen Species; D014812:Vitamin K / Q000494:pharmacology; D014934:Wood; D047309:Flavones
D001205:Ascorbic Acid; D002839:Chromans; D005419:Flavonoids; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D008353:Mannitol; D017382:Reactive Oxygen Species; D014812:Vitamin K; C008548:morin; D047309:Flavones; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid