Expression of the soluble lectin L-14 gene is induced by TSH in thyroid cells and suppressed by retinoic acid in transformed neural cells.
Expression of the soluble lectin L-14 is low in normal and very high in transformed thyroid cells. We show that L-14 gene expression is transiently induced upon thyrotropin stimulation of normal quiescent FRTL-5 rat thyroid cells. Permanent activation of L-14 gene expression is obtained in the same cells infected with a wild-type and a temperature sensitive mutant of Kirsten murine sarcoma virus, both at the permissive and non permissive temperature for transformation. We also find that L-14 mRNA is undetectable in rat brain but is abundant in rat oligodendrocytes precursors transformed by polyoma middle T oncogene. Retinoic acid treatment of these transformed cells leads to acquisition of a differentiated phenotype accompanied by a 30-fold decrease of L-14 mRNA levels. Removal of retinoic acid restores both the transformed undifferentiated phenotype and high L-14 expression. Taken together these results indicate that growth stimulation and induction of cell differentiation are accompanied by strong modulation of L-14 gene expression.
Chiariotti
L
L
Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università degli Studi di Napoli, Italy.
Benvenuto
G
G
Salvatore
P
P
Veneziani
B M
BM
Villone
G
G
Fusco
A
A
Russo
T
T
Bruni
C B
CB
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism; D037161:Galectins; D015870:Gene Expression / Q000187:drug effects*; D006388:Hemagglutinins / Q000096:biosynthesis*; D007700:Kinetics; D007708:Kirsten murine sarcoma virus / Q000235:genetics*; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000187:drug effects / Q000378:metabolism*; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*
D002352:Carrier Proteins; D037161:Galectins; D006388:Hemagglutinins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014212:Tretinoin; D013972:Thyrotropin; D004247:DNA; D013936:Thymidine