title: Lipoprotein lipase and metabolic activities in incubated bovine adipose tissue explants: effects of insulin, dexamethasone, and fetal bovine serum.
abstract: An in vitro system was used to study the regulation of lipoprotein lipase (LPL) activity in bovine adipose tissue. The utilization of two energetic and lipogenic substrates, acetate and glucose, and the activity of glucose-6-phosphate dehydrogenase (G6PDH), an enzyme involved in de novo lipogenesis, were also studied. Nine nonlactating, nonpregnant Holstein cows were given limited amounts of feed for 10 d, then they were overfed for 3 to 5 wk. Samples of perirenal adipose tissue were incubated for 24 or 48 h. Insulin (2 mU/mL) increased (P < .001) daily glucose and acetate utilization and attenuated (P < .001) the loss of G6PDH activity detected after 1 or 2 d of incubation. Dexamethasone (DEX, 10 nM) added to the insulin-supplemented medium decreased (P < .02) glucose utilization, but it did not change acetate utilization or G6PDH activity. A higher concentration of DEX (100 nM) potentiated (P < .004) the ability of insulin to attenuate the decrease in G6PDH activity without changing substrate utilization. Under basal conditions, LPL activity was decreased by approximately 66% after 2 d of incubation. The decline in LPL activity was attenuated by insulin addition (P < .02) and was further attenuated (P < .004) by 100 nM of DEX. The addition of 10% fetal bovine serum alone to the medium had no effect on LPL activity, and fetal bovine serum decreased this activity when it was added to the insulin-supplemented medium.
authors.affiliation: Laboratoire Sous-Nutrition des Ruminants, INRA Theix, Saint Genès Champanelle, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Faulconnier
authors.forename: M
authors.initials: M
authors.lastname: Thévenet
authors.forename: J
authors.initials: J
authors.lastname: Fléchet
authors.forename: Y
authors.initials: Y
authors.lastname: Chilliard
mesh_terms: D000085:Acetates / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D046508:Culture Techniques; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D005312:Fetal Blood / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D008071:Lipoprotein Lipase / Q000378:metabolism
chemical_list: D000085:Acetates; D007328:Insulin; D003907:Dexamethasone; D005954:Glucosephosphate Dehydrogenase; D008071:Lipoprotein Lipase; D005947:Glucose