Muscular cavernous single cell analysis in patients with venoocclusive dysfunction.
Enzymatically isolated smooth muscle cells of the corpora cavernosa obtained from open biopsies of 15 patients, clinically nonresponding to papaverine/phentolamine and prostaglandin E1 (PGE1) and classified by cavernosometry, were examined using the patch-clamp technique in the whole-cell configuration mode simultaneously monitoring the intracellular calcium concentration by means of the Ca(2+)-sensitive fluorescence dye FURA-II. It could be demonstrated that extracellularly applied PGE1 induces smooth muscle relaxation by inhibition of voltage-dependent L-type Ca2+ currents (58 +/- 8%). Compared to intact cavenous tissue (n = 5), the smooth muscle cells of 14/15 PGE1 nonresponders had no evidence of functional disturbance. Due to intact smooth muscle cells in most cases, etiology of venoocclusive dysfunction remains unclear.
Derouet
H
H
Eckert
R
R
Trautwein
W
W
Ziegler
M
M
D000527:Alprostadil / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D007172:Erectile Dysfunction / Q000209:etiology / Q000473:pathology*; D016257:Fura-2; D006801:Humans; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000187:drug effects / Q000473:pathology*; D010409:Penile Diseases / Q000473:pathology*; D010410:Penile Erection / Q000502:physiology*; D010413:Penis / Q000098:blood supply / Q000473:pathology; D014652:Vascular Diseases / Q000150:complications / Q000473:pathology*
D015220:Calcium Channels; D000527:Alprostadil; D016257:Fura-2