Schistosoma mansoni: determination of the sex of cercariae using a nonradioactively labelled DNA probe.
Cercarial sex determination protocol which utilizes nonradioactive nucleic acid labelling with digoxigenin (DIG)-11-dUTR, offers a rapid "clean" identification of the sex of Schistosoma mansoni cercaria. DIG labelled pW1-DNA (plasmid female-specific genomic DNA clone--W1) hybridized with the native cercarial DNA, and hybridization signals are visualized with Lumigen PPD. Results obtained are comparable with those obtained with the 32P labelled pW1.
Giboda
M
M
Institute of Parasitology, McGill University, Quebec, Canada.
Campos
E G
EG
Smith
J M
JM
Prichard
R K
RK
D000818:Animals; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D004076:Digoxigenin; D005260:Female; D008297:Male; D009693:Nucleic Acid Hybridization; D012550:Schistosoma mansoni / Q000235:genetics*; D012732:Sex Determination Analysis
D015342:DNA Probes; D004247:DNA; D004076:Digoxigenin