title: Expression and stability of recombinant RQ-mRNAs in cell-free translation systems.
abstract: Expression of dihydrofolate reductase (DHFR) and chloramphenicol acetyltransferase (CAT) mRNAs in cell-free Escherichia coli translation systems is greatly enhanced as a result of their insertion into RQ135 RNA, a naturally occurring satellite of phage Q beta. The enhancement is due to protection of the recombinant mRNAs against endogenous ribonucleases and to an increased initial rate of translation in the case of the RQ-CAT mRNA.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Puschino, Moscow Region.
authors.forename: V I
authors.initials: VI
authors.lastname: Ugarov
authors.lastname: Morozov IYu
authors.forename: G Y
authors.initials: GY
authors.lastname: Jung
authors.forename: A B
authors.initials: AB
authors.lastname: Chetverin
authors.forename: A S
authors.initials: AS
authors.lastname: Spirin
mesh_terms: D017908:Allolevivirus / Q000235:genetics*; D002474:Cell-Free System; D015500:Chloramphenicol O-Acetyltransferase / Q000037:antagonists & inhibitors; D003001:Cloning, Molecular; D005493:Folic Acid Antagonists; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D005493:Folic Acid Antagonists; C028696:Qbeta ribonucleic acid; D012333:RNA, Messenger; D012367:RNA, Viral; D015500:Chloramphenicol O-Acetyltransferase