Photosensitized cross-linking and cleavage of pBR322 and M13 DNA: comparison of 4,4',6-trimethylangelicin and 3-carbethoxypsoralen.
The furocourmarins 3-carbethoxypsoralen (3-CP) and 4,4',6-trimethylangelicin (TMA) were generally believed to be incapable of cross-linking DNA upon irradiation with ultraviolet light. Denaturation of photosensitized pBR322 DNA, either supercoiled or previously linearized with a restriction enzyme, proved that 3-CP was indeed monofunctional, but that TMA produced cross-links. Identical conclusions were reached with double stranded M13 DNA which had been linearized with EcoR 1. Both sensitizers also induced partial DNA cleavage. In contrast to 3-CP, photosensitization with TMA made the DNA resistant to enzymatic cleavage.
Chen
X
X
Department of Chemistry, University of Illinois at Chicago 60607-7061.
Kagan
J
J
Miolo
G
G
Dall'Acqua
F
F
Averbeck
D
D
Bisagni
E
E
D017104:Bacteriophage M13; D004249:DNA Damage; D004279:DNA, Viral / Q000187:drug effects* / Q000528:radiation effects; D015252:Deoxyribonucleases, Type II Site-Specific; D011564:Furocoumarins / Q000633:toxicity*; D008027:Light; D008730:Methoxsalen / Q000633:toxicity; D009691:Nucleic Acid Denaturation; D010778:Photochemotherapy; D010957:Plasmids / Q000187:drug effects* / Q000528:radiation effects; D011838:Radiation-Sensitizing Agents / Q000633:toxicity*; D015183:Restriction Mapping
D004279:DNA, Viral; D011564:Furocoumarins; D011838:Radiation-Sensitizing Agents; C081211:7-methylpyrido(4,3-c)psoralen; C017104:3-carbethoxypsoralen; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C041621:4,4',6-trimethylangelicin; D008730:Methoxsalen