Preimplantation single cell analyses of dystrophin gene deletions using whole genome amplification.
Primer extension preamplification (PEP) increases the scope and capacity of single cell genetic diagnosis by generating sufficient template to perform multiple subsequent DNA analyses using the polymerase chain reaction. We report the simultaneous analysis of single cells at five commonly deleted dystrophin exons and at the ZFX/ZFY loci. Ninety three percent of PEP reactions with single amniocytes, chorionic villus cells and blastomeres were successful, and a blinded analysis of single lymphoblasts from affected males resulted in 93% diagnostic accuracy, demonstrating its applicability in preimplantation prevention of Duchenne muscular dystrophy. Transfer of unaffected male embryos and improved diagnostic reliability are achieved with the ability to perform replicate multilocus analyses from the same blastomere.
Kristjansson
K
K
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
Chong
S S
SS
Van den Veyver
I B
IB
Subramanian
S
S
Snabes
M C
MC
Hughes
M R
MR
D001483:Base Sequence; D001755:Blastocyst / Q000166:cytology; D017931:DNA Primers / Q000235:genetics; D016189:Dystrophin / Q000235:genetics*; D047108:Embryonic Development; D005260:Female; D005784:Gene Amplification; D017353:Gene Deletion; D015894:Genome, Human; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009136:Muscular Dystrophies / Q000175:diagnosis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012732:Sex Determination Analysis
D017931:DNA Primers; D016189:Dystrophin