Discrete stimulatory effects of platelet-derived growth factor (PDGF-BB) on Leydig cell steroidogenesis.
The effect of platelet-derived growth factor-BB on adult rat Leydig cell steroidogenesis has been determined using culture conditions which maintain testosterone production and responsiveness to luteinising hormone for 3 days. A significant stimulation of testosterone levels was observed in acute response to a maximally stimulating dose (8 ng/ml) of rat luteinising hormone on the third day of culture, but was dependent on pre-exposure of the Leydig cells to PDGF-BB for 2 days, whilst maintained in the presence of a minimum dose (0.1 ng/ml) of rat luteinising hormone. These data demonstrate a very discrete action of PDGF-BB on adult rat Leydig cell steroidogenesis in vitro, but whether or not this constitutes a significant paracrine effect in vivo remains to be established.
Risbridger
G P
GP
Institute of Reproduction & Development, Monash University, Monash Medical Centre, Clayton, Victoria, Australia.
D000818:Animals; D000077214:Becaplermin; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017479:Receptors, Platelet-Derived Growth Factor / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D013268:Stimulation, Chemical; D013739:Testosterone / Q000096:biosynthesis*
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D000077214:Becaplermin; D013739:Testosterone; D017479:Receptors, Platelet-Derived Growth Factor