Molecular cloning and sequence analysis of the gene of the molybdenum-containing aldehyde oxido-reductase of Desulfovibrio gigas. The deduced amino acid sequence shows similarity to xanthine dehydrogenase.
In this report, we describe the isolation of a 4020-bp genomic PstI fragment of Desulfovibrio gigas harboring the aldehyde oxido-reductase gene. The aldehyde oxido-reductase gene spans 2718 bp of genomic DNA and codes for a protein with 906 residues. The protein sequence shows an average 52% (+/- 1.5%) similarity to xanthine dehydrogenase from different organisms. The codon usage of the aldehyde oxidoreductase is almost identical to a calculated codon usage of the Desulfovibrio bacteria.
Thoenes
U
U
Instituto Gulbekian de Ciênca, Laboratório de Genética Molecular, Oeiras, Portugal.
Flores
O L
OL
Neves
A
A
Devreese
B
B
Van Beeumen
J J
JJ
Huber
R
R
Romão
M J
MJ
LeGall
J
J
Moura
J J
JJ
Rodrigues-Pousada
C
C
D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D003901:Desulfovibrio / Q000201:enzymology* / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D008982:Molybdenum; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014968:Xanthine Dehydrogenase / Q000235:genetics
D017931:DNA Primers; D008982:Molybdenum; D014968:Xanthine Dehydrogenase; D000445:Aldehyde Oxidoreductases