Macrophage treatment in suckling rat endotoxic shock.
Gram-negative sepsis/septic shock in the human newborn continues to be a severe medical problem because of significant mortality and morbidity. Since macrophages detoxify endotoxin, a decreased number of macrophages may contribute to the newborn's sensitivity to endotoxin. In this study, peritoneal macrophages were used for the treatment of endotoxic shock in 10-day-old rats, and 24-hr mortality, plasma glucose, and lactate concentrations were monitored. Peritoneal macrophages were harvested from adult or 10-day-old rats. Caseinate-stimulated macrophages from adult and 10-day-old rats significantly decreased the mortality of 10-day-old rat endotoxic shock from 90% to 37.5% and 44.4%, respectively. Resident macrophages from adult and 10-day-old rats also decreased the mortality from 90% to 12.5% and 45.4%, respectively. Peritoneal macrophages from adult rats significantly ameliorated hypoglycemia during endotoxic shock in a dose-dependent manner. Macrophage treatment decreased plasma endotoxin concentration (P < 0.05). Macrophage treatment was important for host defense.
Goto
M
M
Department of Pediatrics, Stritch School of Medicine, Loyola University of Chicago, Maywood, Illinois.
Lichtenberg
R C
RC
Gottschalk
M E
ME
Anderson
C L
CL
Mathews
H L
HL
Zeller
W P
WP
D000818:Animals; D000833:Animals, Suckling; D001786:Blood Glucose / Q000032:analysis; D002452:Cell Count; D005260:Female; D007773:Lactates / Q000097:blood; D008070:Lipopolysaccharides / Q000097:blood / Q000633:toxicity; D008264:Macrophages / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000628:therapy*
D001786:Blood Glucose; D007773:Lactates; D008070:Lipopolysaccharides