Cloning and sequencing of an Na+/H+ antiporter gene from the marine bacterium Vibrio alginolyticus.
A gene has been cloned from a DNA library from the marine bacterium Vibrio alginolyticus that functionally complements a mutant strain of Escherichia coli, NM81, defective in an Na+/H+ antiporter (NhaA). The cloned Vibrio gene restored NM81 to grow in a medium containing 0.5 M NaCl at pH 7.5 and concomitantly led to an increase in Na+/H+ antiport activity. The nucleotide sequence of the cloned fragment revealed an open reading frame, which encodes a protein with a predicted 383 amino acid sequence and molecular mass of 40,400 Da. The hydropathy profile is characteristic of a membrane protein with 11 membrane spanning regions. The deduced amino acid sequence is 58% identical with E. coli NhaA.
Nakamura
T
T
Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Komano
Y
Y
Itaya
E
E
Tsukamoto
K
K
Tsuchiya
T
T
Unemoto
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics*; D014733:Vibrio / Q000235:genetics*
D017923:Sodium-Hydrogen Exchangers