Electrophoretic and serological analysis of host antigens associated with the adult Schistosoma mansoni tegument.
One-dimensional and 2-dimensional (2-D) polyacrylamide gel electrophoresis and immunoblotting with various specific antisera were used to analyze host antigens eluted from the surface of adult male Schistosoma mansoni into culture medium following short-term incubation. Silver-stained sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels revealed 27 protein bands from the parasite culture medium (eluate), ranging from > 200 kDa to 25 kDa. Eight of these bands corresponded to whole mouse plasma protein bands that migrated to the same positions. Color silver-stained 2-D SDS-PAGE gels revealed 93 protein spots from the eluate; 32 of them corresponded with similar spots in a normal mouse serum 2-D SDS-PAGE gel. Eleven protein components of the eluate, ranging from > 200 kDa to 25 kDa, reacted with anti-mouse whole serum antiserum. Specific antisera recognizing components in the parasite culture medium included those with specificity for each of the classes and subclasses of mouse immunoglobulin (except IgG and IgE), mouse albumin, and mouse alpha-2-macroglobulin. Specific antisera against murine erythrocytes, hemoglobin, the complement component C3, transferrin, fibronectin, fibrinogen, and the mouse major histocompatibility class I protein H-2Db heavy chain did not react with any eluate component.
Gearner
G W
GW
Department of Biology, College of Science, Texas A&M University, College Station 77843.
Kemp
W M
WM
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D012550:Schistosoma mansoni / Q000276:immunology*
D000947:Antigens, Helminth; D000954:Antigens, Surface; D007106:Immune Sera