Gender determination by multiplex PCR amplification of alphoid repeat sequences from single cells.
Alphoid repeat sequences on the X and Y chromosomes were successfully and consistently coamplified to determine the sex of single cells. Buccal cells served as a convenient model for single blastomeres obtained from preimplantation embryos through in vitro fertilization. The potential use of this technique for preimplantation genetic diagnosis is discussed.
Steuerwald
N
N
San Francisco Center for Reproductive Medicine, CA 94118.
Lambert
H
H
Steinleitner
A J
AJ
Herrera
R J
RJ
D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012732:Sex Determination Analysis