Uptake of cobalamin by Euglena mitochondria.
Cobalamin uptake by Euglena mitochondria is a biphasic process, consisting of energy-independent cobalamin-binding to mitochondrial membranes and energy-dependent active transport. The energy-dependent phase of cobalamin uptake is not dependent on mitochondrial respiration, but on the presence of ATP within the mitochondrial matrix. The dissociation constant of the energy-independent cobalamin-binding reaction is estimated to be 0.45 nM. Inhibition of the mitochondrial cobalamin uptake by a variety of cobalamin analogues indicates that Euglena mitochondria have an absolute requirement for the complete cobalamin molecule with an alpha-axial ligand (the cobalt-coordinated nucleotide) and an intact b-propionamide side-chain. Thus, the Euglena mitochondrial cobalamin uptake system is highly specific for the cobalamin structure. The cobalamin taken up by the Euglena mitochondria cannot be exchanged with exogenous cobalamin. All of the mitochondrial cobalamin is associated with three proteins with molecular masses of > 700,000 (16.3%), 160,000 (7.4%), and 35,000 (76.3%). They occur in the soluble fraction of mitochondria, suggesting that these cobalamin-binding proteins or cobalamin-dependent enzymes play an important role in cobalamin accumulation and metabolism within the mitochondria.
Watanabe
F
F
Laboratory of Nutrition and Food Science, Hagoromo-gakuen College, Osaka.
Tamura
Y
Y
Stupperich
E
E
Nakano
Y
Y
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D005056:Euglena gracilis / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D010101:Oxygen Consumption / Q000187:drug effects; D013696:Temperature; D014805:Vitamin B 12 / Q000031:analogs & derivatives / Q000378:metabolism*
D000255:Adenosine Triphosphate; D014805:Vitamin B 12