Purification, characterization and biological activities of phospholipase A from Russell's viper (Vipera russelli) venom.
1. The major phospholipase A has been purified to electrophoretic homogeneity from the venom of Vipera russelli (Russell's viper). 2. The molecular weight of the purified enzyme was estimated to be 31,000 by Sephadex G-75 gel filtration chromatography and 29,000 by SDS-polyacrylamide gel electrophoresis. The enzyme exhibited an apparent Km value of 2.3 x 10(-2) M. 3. The phospholipase A showed edema forming, indirect hemolytic and myonecrotic activities but not hemorrhagic activity.
Aye-Kyaw
Biochemistry Department, Institute of Medicine, Mandalay, Myanmar, Burma.
San-Aye
Tin-Win
Hla-Pe
Mya-Maung
D000818:Animals; D004487:Edema / Q000139:chemically induced; D006461:Hemolysis / Q000187:drug effects; D006470:Hemorrhage; D051379:Mice; D008970:Molecular Weight; D009336:Necrosis; D010741:Phospholipases A / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D014757:Viper Venoms / Q000201:enzymology*
D014757:Viper Venoms; D010741:Phospholipases A