title: The significance of nucleolar organizer region (AgNOR) score in predicting meningioma recurrence.
abstract: BACKGROUND
  Argyrophilic nucleolar organizer region (AgNOR) has been demonstrated in recent studies of human brain tumors, including meningiomas. The authors used this technique in meningiomas to analyze whether the mean numbers of AgNOR per nucleus (AgNOR score) are related to the recurrence rate or the proliferative potential of meningiomas.
  
  
  METHODS
  AgNOR staining technique was applied to paraffin-embedded sections of 60 meningiomas. Twenty of these specimens also were investigated immunohistochemically with monoclonal antibody (MoAb) against DNA polymerase alpha (Pol.alpha) and with MoAb Ki-67 to compare the AgNOR score with the proliferative potential.
  
  
  RESULTS
  There was a statistically significant difference between the AgNOR score in nonrecurrent meningiomas (2.48 +/- 0.73) and recurrent histologically benign meningiomas after gross total resection (3.20 +/- 0.96) (P < 0.02). The recurrence rate of tumors after gross total resection with an AgNOR score of 3.0 or more was significantly higher than that with an AgNOR score of less than 3.0. The AgNOR score did not always correlate with Pol.alpha or Ki-67 score; the AgNOR score of malignant meningioma that had high proliferative score was not always high.
  
  
  CONCLUSIONS
  This study indicates that AgNOR staining appears to be a simple and useful method for estimating the probability of histologically benign meningioma recurrence even after gross total resection.
authors.affiliation: Department of Neurological Surgery, Okayama University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kunishio
authors.forename: T
authors.initials: T
authors.lastname: Ohmoto
authors.forename: T
authors.initials: T
authors.lastname: Matsuhisa
authors.forename: T
authors.initials: T
authors.lastname: Maeshiro
authors.forename: T
authors.initials: T
authors.lastname: Furuta
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000502:physiology; D002467:Cell Nucleus / Q000737:chemistry; D004257:DNA Polymerase II / Q000032:analysis / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D008297:Male; D008577:Meningeal Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D008579:Meningioma / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D009364:Neoplasm Recurrence, Local / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000032:analysis; D009697:Nucleolus Organizer Region / Q000737:chemistry; D016612:Paraffin Embedding; D011237:Predictive Value of Tests; D011379:Prognosis; D012189:Retrospective Studies; D016622:Silver Staining
chemical_list: D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D004257:DNA Polymerase II