Passive transfer of adjuvant-induced arthritis into irradiated DA recipient rats.
Adjuvant-induced arthritis (AIA) can be passively transferred in Dark Agouti (DA) rats by spleen and lymph node cells after culture with Concanavalin A (Con A). A model not requiring in vitro Con A expansion and activation would be important in investigations of anti-rheumatic drugs in AIA. A new model using irradiated recipients fills this need. Donor DA rats treated with 0.1 ml complete Freund's adjuvant (CFA) containing 7.5 mg M. butyricum/ml were sacrificed 11 days after CFA injection, donor spleen cells harvested, and donor spleen cells injected intravenously into recipient DA rats previously irradiated with 5 Gy. Recipient rats developed arthritis 4-14 days after spleen cell transfer. This model can now be used to further define the effects of anti-rheumatic drugs in the passive transfer of AIA by eliminating the need for the in vitro Con A-induced expansion and/or activation of donor cells.
Cannon
G W
GW
Salt Lake City Veterans Affairs Medical Center, Utah 84148.
Harper
D S
DS
Clayton
F
F
Griffiths
M M
MM
D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology* / Q000473:pathology; D017690:Cell Transplantation; D005620:Freund's Adjuvant; D016219:Immunotherapy, Adoptive; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology; D014916:Whole-Body Irradiation
D005620:Freund's Adjuvant