title: Further study on the magnesium-mediated change in physical state of phospholipid modulates mitochondrial F0-F1-ATPase activity.
abstract: We have postulated that magnesium may play a role in altering the lipid fluidity of the bilayers, which would induce a change of conformation of the F0-ATPase portion (buried in the lipid core) of mitochondrial F0-F1-ATPase. Such change could be transmitted to the soluble F1 portion, resulting in higher enzymatic activity. The assumption was further supported by the results presented in the following: (1) A conformational difference for the F0-ATPase-containing proteoliposomes induced by the magnesium effect could be detected using a fluorescent probe acrylodan; (2) H(+)-translocation activity of F0-ATPase-incorporated proteoliposomes with magnesium, monitoring by fluorescence quenching of 9-aminoacridine or the bulk phase pH change, was higher than that without magnesium; (3) The magnesium effect on the reconstituted F0-F1-ATPase activity was greatly enhanced when the reconstitution was carried out in the presence of oligomycin sensitivity conferring protein (OSCP, a main component of the connecting link between the F1 and F0 sector of F0-F1-ATPase).
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Tong
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: F
authors.initials: F
authors.lastname: Yang
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives; D000818:Animals; D007425:Intracellular Membranes / Q000187:drug effects / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology* / Q000502:physiology; D008560:Membrane Fluidity / Q000187:drug effects; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology; D008968:Molecular Conformation; D010743:Phospholipids / Q000737:chemistry*; D006180:Proton-Translocating ATPases / Q000187:drug effects* / Q000378:metabolism; D013552:Swine
chemical_list: D010743:Phospholipids; C038518:acrylodan; D015081:2-Naphthylamine; D006180:Proton-Translocating ATPases; D008274:Magnesium