title: Elevation of the phospholipase A2 activity in peritoneal fluid cells from women with endometriosis.
abstract: OBJECTIVE
  To assess the prostaglandin (PG) production on peritoneal fluid (PF) cells, phospholipase A2 (PLA2) activity of those cells in women with endometriosis was measured and compared with that of women without endometriosis.
  
  
  METHODS
  Prospective clinical controlled study.
  
  
  METHODS
  Women who underwent laparoscopy and were found either to have endometriosis (n = 15) or not (n = 9) were included in this study. Mononuclear cells obtained from the patients at laparoscopy were immediately separated by a Ficoll-Paque technique, lysed by nitrogen cavitation, and stored at -80 degrees C.
  
  
  METHODS
  Phospholipase A2 activity was measured by Dole assay using 1-palmitoyl-2-[1-14C] palmitoyl phosphatidyl choline and assessed on a protein basis and a cell number basis.
  
  
  RESULTS
  There were at least four measurable kinds of PLA2 activity detected in the cells: two calcium-dependent pH optima 7.0 and 9.0 activities and two calcium-independent pH optima 7.5 and 8.5 activities. A calcium-dependent and pH optima 9.0 activity was the highest, and it was significantly higher in women with endometriosis when compared with those who did not have endometriosis.
  
  
  CONCLUSIONS
  These results indicate that the increase in the PGs in PF with endometriosis may be produced by PF cells in which PLA2 activity is elevated.
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sano
authors.forename: T
authors.initials: T
authors.lastname: Morishita
authors.forename: M
authors.initials: M
authors.lastname: Nozaki
authors.forename: M
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Nakano
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000166:cytology* / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology; D004715:Endometriosis / Q000201:enzymology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D017737:Macrophages, Peritoneal / Q000201:enzymology; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011446:Prospective Studies
chemical_list: D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium