Bestatin transport in rabbit intestinal brush-border membrane vesicles.
The effect of papain treatment on bestatin uptake by rabbit intestinal brush-border membrane vesicles (BBMVs) was studied. Papain treatment of BBMVs effectively diminished aminopeptidase activity but not bestatin uptake by a H+/dipeptide cotransporter. Bestatin uptake by BBMVs was composed of two saturable components, and after papain treatment the high-affinity component disappeared while the low-affinity component was retained. These findings suggest that high- and low-affinity components represent bestatin binding to aminopeptidase and the true uptake by the H+/dipeptide cotransporter, respectively.
Takano
M
M
Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Japan.
Tomita
Y
Y
Katsura
T
T
Yasuhara
M
M
Inui
K
K
Hori
R
R
D000276:Adjuvants, Immunologic / Q000493:pharmacokinetics*; D000818:Animals; D001692:Biological Transport; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism; D010206:Papain / Q000494:pharmacology; D011817:Rabbits
D000276:Adjuvants, Immunologic; D010206:Papain; D007930:Leucine; C012211:ubenimex