title: Solubilization, characterization and photoaffinity labeling of the mitochondrial dihydropyridine receptor from bovine adrenal medulla.
abstract: 1. The mitochondrial dihydropyridine receptor was solubilized with Chaps at a detergent/protein ratio of 2.5, during 45 min at 4 degrees C. 2. From the rate constants of association (8.10 +/- 0.25 x 10(4) M-1 min-1) and dissociation (0.022 +/- 0.001 min-1) a Kd of 275 nM was calculated, while from saturation experiments a Kd of 270 +/- 30 nM and a density of receptors of 106 +/- 9 pmol/mg protein was obtained. 4. The solubilized receptors are heat-resistant, sensitive to the trypsin and to the reduction of disulfide bonds. 5. In native membranes, a polypeptide of 50 kDa was specifically photolabelled with [3H]Azidopine.
authors.affiliation: Departamento de Neuroquímica, Universidad de Alicante, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hidalgo
authors.forename: L M
authors.initials: LM
authors.lastname: Gutierrez
authors.forename: M
authors.initials: M
authors.lastname: Palmero
authors.forename: J J
authors.initials: JJ
authors.lastname: Ballesta
authors.forename: J A
authors.initials: JA
authors.lastname: Reig
authors.forename: S
authors.initials: S
authors.lastname: Viniegra
mesh_terms: D000313:Adrenal Medulla / Q000737:chemistry* / Q000648:ultrastructure; D000818:Animals; D020746:Calcium Channels, L-Type; D002417:Cattle; D008928:Mitochondria / Q000737:chemistry*; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry*; D012995:Solubility; D014316:Tritium
chemical_list: D020746:Calcium Channels, L-Type; D009124:Muscle Proteins; D014316:Tritium