Pharmacological actions of the coenzymes NAD(H) and NADP(H) on the rat anococcygeus muscle.
1. The pharmacological actions of the oxidized and reduced forms of nicotinamide-adenosine dinucleotide (NAD, NADH) and nicotinamide-adenosine dinucleotide phosphate (NADP, NADPH) were studied on rat isolated anococcygeus muscles. 2. The actions of the two nucleotides were different, but there were no apparent qualitative differences between the oxidized and reduced forms of each. 3. In fully relaxed anococcygeus muscles, NADP(H) produced transient contractions that were subject to desensitization, but NAD(H) had no effect. 4. NADP(H) slightly enhanced contractions elicited by noradrenergic nerve stimulation. In contrast, noradrenergic contractions were inhibited by NAD(H). NADH reduced the stimulation-induced release of noradrenaline, but enhanced contractions elicited by exogenous noradrenaline. 5. In anococcygeus muscles partly contracted with guanethidine, NAD(H) produced a further sustained increase in tone; in contrast, NADP(H) mainly produced transient relaxations to which there was immediate desensitization. 6. Relaxations of anococcygeus muscle elicited by nitrergic nerve stimulation were not affected by NAD. In contrast, NADP(H) reduced them. 7. The actions of NAD(H) were generally the same as those of adenosine and can be attributed to activation of P1-purinoceptors since they were blocked by the selective antagonist 8-sulphophenyltheophylline. 8. The actions of NADP resembled those of the P2-purinoceptor agonist ATP to some extent, but there were some differences. As suggested by others, NADP may act on a unique receptor.
Najbar
A
A
Department of Pharmacology, University of Melbourne, Parkville, Victoria, Australia.
Li
C G
CG
Rand
M J
MJ
D000241:Adenosine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004558:Electric Stimulation; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009243:NAD / Q000494:pharmacology*; D009249:NADP / Q000494:pharmacology*; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000502:physiology; D009638:Norepinephrine / Q000494:pharmacology; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology
D009243:NAD; D009569:Nitric Oxide; D009249:NADP; C031433:8-(4-sulfophenyl)theophylline; D001120:Arginine; D013806:Theophylline; D000241:Adenosine; D019331:NG-Nitroarginine Methyl Ester; D009638:Norepinephrine