SS33410, an inhibitor for inflammation, blocks the intracellular transport of VSV G glycoprotein in BHK cells.
We have studied the effect of SS33410, an inhibitor for inflammation, on the intracellular transport and processing of the vesicular stomatitis virus (VSV) G glycoprotein as a model integral membrane protein. Delivery of G glycoprotein to the cell surface was blocked by 0.5 microgram/ml of SS33410 without any significant inhibition of protein synthesis. The G glycoprotein accumulated intracellularly electrophoresed a little faster than the control mature one excreted to the medium. The affinity for concanavalin A-agarose (Con-A) column suggested that most of the G glycoprotein oligosaccharides were of the high-mannose type. These results indicate that processing of N-glycosidic oligosaccharide is incomplete, suggesting that intracellular trafficking is arrested before reaching to the trans Golgi compartments in the presence of SS33410.
Seog
D H
DH
Department of Agricultural Chemistry, University of Tokyo, Japan.
Yamasaki
M
M
Takatsuki
A
A
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002846:Chromatography, Affinity; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D013302:Streptomyces; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014759:Viral Envelope Proteins; D014779:Virus Replication / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins