Pasteurella multocida: oropharyngeal carriage and antibody response in breeders.
The presence of Pasteurella multocida in the oropharynx of 58 pig, cattle or rabbit breeders whose livestock had suffered from pasteurellosis was investigated using a selective medium. Blood samples for serological studies were collected at the same moment. Nineteen breeders were found to host one P multocida subsp multocida strain. Oropharyngeal carriage of P multocida was found to be more frequent in pig breeders (42% of individuals) than in cattle (10%) or rabbit (0%) breeders. Genomic polymorphism among 10 D2 strains was found by restriction endonuclease analysis using pulsed-field gel electrophoresis (REA-PFGE). Antibodies to P multocida were found in the sera of 32 of these 58 breeders, whereas only 2 of the 70 controls had antibodies. These results, recorded from healthy individuals, show that P multocida, acting as an opportunistic bacterium, may be responsible for occupational diseases. Nevertheless, the strong prevalence of specific antibodies makes the presence of antibodies in the sera of these breeders an insufficient indication of a current patent infection.
Donnio
P Y
PY
Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Rennes, France.
Le Goff
C
C
Avril
J L
JL
Pouedras
P
P
Gras-Rouzet
S
S
D000328:Adult; D000368:Aged; D000382:Agricultural Workers' Diseases / Q000453:epidemiology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D016667:Bacterial Capsules / Q000276:immunology; D002353:Carrier State / Q000453:epidemiology; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005602:France / Q000453:epidemiology; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D009960:Oropharynx / Q000382:microbiology*; D010326:Pasteurella Infections / Q000453:epidemiology*; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D015995:Prevalence; D000091402:Prohibitins; D011817:Rabbits; D015183:Restriction Mapping; D012703:Serotyping; D013552:Swine
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; C000717331:PHB2 protein, human; D000091402:Prohibitins