title: Assessment of Ki-67-derived tumor proliferative activity in colorectal adenocarcinomas.
abstract: Analysis of tumor growth fraction (TGF) has become essential as cell cycle-directed modalities have been increasingly used for the treatment of solid neoplasms. We studied TGF in fresh tissue samples from 74 surgically resected colorectal carcinomas (50 colon and 24 rectum; 44 men and 30 women) by flow cytometry (FCM) and by immunostaining using Ki-67 monoclonal antibody. In 12 cases, samples of uninvolved colonic mucosa adjacent to tumor (transitional mucosa) and samples of normal mucosa (at least 5 cm away from tumor) were available for growth fraction analysis. The mean Ki-67 and S-phase values were 17.1% (range, 0-60%) and 17.5% (range, 3-39%), respectively. The mean percentage of Ki-67 positivity in tumor samples from women was significantly lower than that in samples from men (P = 0.001). Ki-67-derived TGF values, however, did not correlate with patient age, lymph node status, or tumor size, site, stage, degree of differentiation, or DNA ploidy. The correlation between Ki-67-derived and FCM-derived TGF values was statistically significant (P = 0.001) but marginal (R = 0.35). In both transitional and normal colonic mucosa samples, Ki-67 positivity was mainly confined to the lower half of the crypts, and their growth fraction values were significantly lower than those of tumor tissue; however, there was no difference in values between transitional and normal mucosae. Our results indicate that Ki-67-derived TGF does not correlate with known prognostic factors for colorectal carcinoma; however, long-term follow-up information will be necessary to define the clinical value of Ki-67 staining.
authors.affiliation: Department of Pathology, University of Texas, M.D. Anderson Cancer Center, Houston.
authors.forename: A A
authors.initials: AA
authors.lastname: Sahin
authors.forename: J Y
authors.initials: JY
authors.lastname: Ro
authors.forename: R W
authors.initials: RW
authors.lastname: Brown
authors.forename: N G
authors.initials: NG
authors.lastname: Ordonez
authors.forename: K R
authors.initials: KR
authors.lastname: Cleary
authors.forename: A K
authors.initials: AK
authors.lastname: el-Naggar
authors.forename: P
authors.initials: P
authors.lastname: Wilson
authors.forename: A G
authors.initials: AG
authors.lastname: Ayala
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division / Q000502:physiology*; D015179:Colorectal Neoplasms / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000473:pathology; D019394:Ki-67 Antigen; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis / Q000502:physiology*; D009687:Nuclear Proteins / Q000032:analysis / Q000502:physiology*
chemical_list: D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins