title: Purification and some properties of an IMP-specific 5'-nucleotidase from yeast.
abstract: An IMP-hydrolysing enzyme was purified to homogeneity from yeast extract. It was a soluble protein with an apparent molecular mass of 220 kDa, with a subunit molecular mass of 55 kDa. It was highly specific for IMP, and there was virtually no detectable activity with the other purine and pyrimidine nucleotides tested, including AMP and dIMP. The enzyme had a pH optimum of 6.0-6.5. Its activity was absolutely dependent on bivalent metal salts: Mg2+ was most potent, followed by Co2+ and Mn2+. The velocity/substrate-concentration plot of the enzyme was slightly sigmoidal (h = 1.7) and the s0.5 was 0.4 mM. ATP stimulated the enzyme by decreasing both h and s0.5. Diadenosine tetraphosphate stimulated the enzyme as effectively as ATP. Although the properties of the enzyme are similar to those of the IMP/GMP 5'-nucleotidase identified in various animals [Itoh (1993) Comp. Biochem. Physiol. 105B, 13-19], the substrate specificity of the former was much more strict than the latter.
authors.affiliation: National Institute of Health and Nutrition, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Itoh
mesh_terms: D015720:5'-Nucleotidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology; D002384:Catalysis; D002413:Cations, Divalent; D003035:Cobalt / Q000494:pharmacology; D015226:Dinucleoside Phosphates / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007291:Inosine Monophosphate / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D002413:Cations, Divalent; D015226:Dinucleoside Phosphates; D007291:Inosine Monophosphate; D003035:Cobalt; D008345:Manganese; C020733:diadenosine tetraphosphate; D000255:Adenosine Triphosphate; D015720:5'-Nucleotidase; D008274:Magnesium