Subcellular localization of Pfs16, a Plasmodium falciparum gametocyte antigen.
We have used immunoelectron microscopy to investigate the subcellular location of Pfs16 in Plasmodium falciparum. It was detected in the outer membrane region of gametocytes and more specifically on the parasitophorous vacuole membrane (pvm), since, during gametogenesis when the pvm disintegrates, the majority of the antigen was detected on the remains of this membrane in multilaminated whorls and not on the gamete plasma membrane. The antigen was also present on other gametocyte cellular structures, including those which we believe to be Garnham bodies, present in the host cell cytoplasm of some gametocytes. The antigen was present too on the membrane surrounding cytosomes and the resulting food vacuoles in the parasite cytoplasm.
Baker
D A
DA
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine.
Daramola
O
O
McCrossan
M V
MV
Harmer
J
J
Targett
G A
GA
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D016253:Microscopy, Immunoelectron; D010963:Plasmodium falciparum / Q000276:immunology* / Q000648:ultrastructure; D014617:Vacuoles / Q000276:immunology
D000953:Antigens, Protozoan; D008565:Membrane Proteins; C068959:Pfs16 protein, Plasmodium falciparum