Isolation and biological characterization of staphyloferrin B, a compound with siderophore activity from staphylococci.
A highly hydrophilic compound with siderophore activity has been isolated from the supernatant of Staphylococcus hyicus DSM 20459 grown under iron-restricted conditions. The metabolite, named staphyloferrin B, is strictly iron-regulated and produced by a large variety of staphylococci strains. In vivo iron transport measurements and the growth-promoting activity in a bioassay establish staphyloferrin B as the second siderophore for staphylococci besides the previously described staphyloferrin A. The structure elucidation revealed 2,3-diaminopropionic acid, citrate, ethylenediamine and 2-ketoglutaric acid as structural components of the compound. Thus, staphyloferrin B is a structurally new siderophore of the complexone type.
Haag
H
H
Universität Tübingen, Frg.
Fiedler
H P
HP
Meiwes
J
J
Drechsel
H
H
Jung
G
G
Zähner
H
H
D001681:Biological Assay; D001692:Biological Transport; D002951:Citrates / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D009952:Ornithine / Q000031:analogs & derivatives / Q000032:analysis; D010848:Picolinic Acids / Q000494:pharmacology; D011073:Polyamines / Q000737:chemistry* / Q000378:metabolism; D017262:Siderophores / Q000737:chemistry* / Q000378:metabolism; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013210:Staphylococcus / Q000737:chemistry* / Q000187:drug effects
D002951:Citrates; D010848:Picolinic Acids; D011073:Polyamines; D017262:Siderophores; C083439:staphyloferrin B; C063851:staphyloferrin A; D007501:Iron; D009952:Ornithine; C030614:picolinic acid