title: Distribution of dystrophin isoforms and dystrophin-associated proteins 43DAG (A3a) and 50DAG (A2) in various monkey tissues.
abstract: To determine the distributions of two known dystrophin isoforms derived from the 3' part of the dystrophin gene and of the dystrophin-associated proteins [50DAG (A2) and 43DAG (A3a)] by immunoblot analysis, we examined various monkey tissues [skeletal (quadriceps), cardiac (left ventricle), and smooth (aorta and uterus) muscles, lung, liver, central nervous system (cerebrum, cerebellum, and spinal cord), and peripheral nerve (sciatic nerve)]. With the antibody against the C-terminal domain of dystrophin, we found three bands other than the 400-kDa band corresponding to dystrophin or utrophin. These are a 130-kDa band detected in central nervous system, a 110-kDa band in peripheral nerve and spinal cord, and a 70-kDa band in all the tissues examined except skeletal muscle and peripheral nerve. The 130-kDa band may be a novel protein not yet reported, while the last two bands may correspond to Dp116 and Dp71, respectively [Lederfein et al. (1992) Proc. Natl. Acad. Sci. USA 89, 5346-5350 and Byers et al. (1993) Nature Genet. 4, 77-81]. Dystrophin-associated protein, 43DAG, was detected in all the tissues examined, but 50DAG was detected only in skeletal and cardiac muscles. Furthermore, a unique 70-kDa band reacting with anti-50DAG antiserum was found in all the tissues other than skeletal muscle.
authors.affiliation: Department of Cell Biology, National Institute of Neuroscience, NCNP, Tokyo.
authors.forename: Y
authors.initials: Y
authors.lastname: Mizuno
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Hirai
authors.forename: E
authors.initials: E
authors.lastname: Ozawa
mesh_terms: D000818:Animals; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000276:immunology; D016189:Dystrophin / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D015151:Immunoblotting / Q000379:methods; D007120:Immunochemistry / Q000379:methods; D007536:Isomerism; D008252:Macaca fascicularis; D008565:Membrane Proteins; D014018:Tissue Distribution; D049411:Utrophin
chemical_list: D003598:Cytoskeletal Proteins; D016189:Dystrophin; D008565:Membrane Proteins; D049411:Utrophin