DNA flow-cytometric analysis of basal cell carcinomas and its relevance to their morphological differentiation: a retrospective study.
DNA flow cytometry (FCM) was performed on archived material of 82 basal cell carcinomas (BCC), using Hedley's technique. Sixty-five samples were analysed. Biopsy samples were classified in five groups depending on their morphological differentiation: solid, cystic, adenoid, keratotic and metatypical. Two parameters were studied: proliferative index and DNA index. Statistical analysis revealed that BCC with keratinizing or metatypical differentiation had a greater amount of aneuploidy when compared with BCC without particular differentiation (solid and cystic) or aneuploid differentiation (p < 0.001). The proliferative indices were significantly different in the four groups. DNA FCM of BCC confirms the value of morphological classifications and gives another example of aneuploidy in a tumour with low aggressiveness.
Fortier-Beaulieu
M
M
Clinique Dermatologique, Centre Hospitalier Régional et Universitaire, Rouen, France.
Laquerrière
A
A
Thomine
E
E
Lauret
P
P
Hémet
J
J
D000782:Aneuploidy; D002280:Basal Cell Carcinoma / Q000737:chemistry / Q000145:classification / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000737:chemistry / Q000145:classification / Q000473:pathology*
D004273:DNA, Neoplasm