Inducible amber suppressor for Bacillus subtilis.
An amber suppressor variant of Bacillus subtilis tyrosyl-tRNA was constructed and placed under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible Pspac promoter. Addition of IPTG resulted in a 50-fold increase in the expression of an rpsD-lacZ fusion containing a UAG amber codon. This system permitted isolation of a conditional lethal mutant which required IPTG for growth.
Grundy
F J
FJ
Department of Biochemistry and Molecular Biology, Albany Medical College, New York 12208.
Henkin
T M
TM
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D003062:Codon; D004790:Enzyme Induction / Q000187:drug effects; D005804:Genes, Lethal / Q000235:genetics; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012365:RNA, Transfer, Tyr / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013489:Suppression, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D003062:Codon; D012365:RNA, Transfer, Tyr; D011993:Recombinant Fusion Proteins; D007544:Isopropyl Thiogalactoside; D001616:beta-Galactosidase