title: [A universal method of purifying genetically engineered human somatotropin and its mutants using metallochelating agent affinity chromatography].
abstract: A novel scheme for human recombinant somatotropin and its mutant forms isolation which includes ion-exchange and metal chelate affinity chromatography has been developed. A change in the chromatographic behaviour of the isolated proteins depending on the amino acid substitutions has been demonstrated.
authors.forename: S A
authors.initials: SA
authors.lastname: Lopatin
authors.forename: A V
authors.initials: AV
authors.lastname: Il'ina
authors.forename: A A
authors.initials: AA
authors.lastname: Shul'ga
authors.forename: O S
authors.initials: OS
authors.lastname: Grinchenko
authors.forename: V P
authors.initials: VP
authors.lastname: Varlamov
authors.forename: K G
authors.initials: KG
authors.lastname: Skriabin
authors.forename: M P
authors.initials: MP
authors.lastname: Kirpichnikov
mesh_terms: D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D013006:Growth Hormone / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008670:Metals; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification
chemical_list: D002614:Chelating Agents; D008670:Metals; D011994:Recombinant Proteins; D013006:Growth Hormone
vernacular_title: Universal'nyĭ sposob ochistki genno-inzhenernogo somatotropina cheloveka i ego mutantov s ispol'zovaniem metallokhelat affinnoĭ khromatografii.