Perfluorooctylbromide (PFOB) as a vitreous substitute in non-human primates.
We evaluated the toxicity of perfluorooctylbromide in the primate eye as a short-term postoperative vitreous substitute. Four eyes of 4 African green monkeys underwent complete vitrectomy and vitreous replacement with 1.5-2.0 ml of PFOB. One additional animal received BSS as a control vitreous substitute in one eye. Animals were examined twice weekly for clarity and consistency of the vitreous replacement substance. Anterior segment and lenses remained clear in all eyes, although in the immediate postoperative period one eye became inflamed and had a culture-negative vitritis. The other eyes showed a minimal anticipated postoperative vitreous inflammation. Emulsification of the PFOB began within 3 days of injection and progressed up to 3 weeks, precluding fundus examination and fluorescein angiography after 2 weeks. Eyes were enucleated and light microscopy performed at 2 days, 10 days, 33 days, and 45 days. No toxic effects to the retinal cells were detectable by histological examination, but perivasculitis of retinal vessels was noted at 45 days. Indirect examination was normal up to 10 days; thereafter, the fundus view was obscured by the emulsified PFOB. Because of cellular migration into the vitreous cavity and retinal perivasculitis, observed histologically, PFOB seems most suitable for intraoperative rather than postoperative use.
Conway
M D
MD
LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112-2234.
Peyman
G A
GA
Karaçorlu
M
M
Bhatt
N
N
Soike
K F
KF
Clark
L C
LC
Hoffmann
R E
RE
D000818:Animals; D000869:Anterior Eye Segment / Q000187:drug effects / Q000473:pathology; D002465:Cell Movement; D002522:Chlorocebus aethiops; D004655:Emulsions; D005069:Evaluation Studies as Topic; D005466:Fluorocarbons / Q000633:toxicity*; D006842:Hydrocarbons, Brominated; D012160:Retina / Q000187:drug effects* / Q000473:pathology; D012171:Retinal Vessels / Q000187:drug effects / Q000473:pathology; D014657:Vasculitis / Q000139:chemically induced / Q000473:pathology; D014821:Vitrectomy / Q000379:methods*; D014822:Vitreous Body
D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; C003072:perflubron