Platelet inhibitory functions of aortic endothelial cells. Effects of eicosapentaenoic and docosahexaenoic acids.
The endothelial cell platelet inhibitory potential was assessed directly by measuring the platelet inhibition induced by platelet interaction with the cultured aortic endothelial cell. The prostacyclin content of the platelet suspensions after interaction was also quantified. We found that prostacyclin production accounted for the overall platelet inhibitory potential of the aortic cells since: (a) endothelial cells incubated with aspirin, which did not produce prostacyclin, did not inhibit platelets; (b) the prostacyclin content of platelet suspensions after interaction with endothelial cells correlated with the extent of the platelet inhibition; (c) such a platelet inhibition was reproduced by adding synthetic prostacyclin in amount equivalent to that produced by endothelial cells during the interaction. Eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids incorporated into endothelial phospholipids, decreased the ability of the cells to produce prostacyclin and to inhibit platelets, DHA being less effective than EPA.
Bénistant
C
C
Inserm U352, Chimie Biologique INSA-Lyon, Villeurbanne, France.
Achard
F
F
Marcelon
G
G
Lagarde
M
M
D000818:Animals; D001011:Aorta / Q000166:cytology*; D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000502:physiology*; D011464:Epoprostenol / Q000096:biosynthesis; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects
D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D011464:Epoprostenol