Alterations of uninfected red blood cells in malaria.
Red blood cell (RBC) negative charges and resistance to linoleic acid (LNA)-induced lysis were studied in Plasmodium yoelii-infected mice and in malaria (P. falciparum)-affected individuals. RBCs from mice infected with P. yoelii showed a progressive decrease in the net surface negative charges at 24 h after infection, reaching a minimal value on day 3, followed by a second phase that was characterised by a recovery to normal levels on day 6. Resistance to linoleic acid follows similar kinetics. These alterations preceded the appearance of parasites in the peripheral blood. A similar increase in LNA-induced lysis was observed in RBCs from malaria-affected individuals. These early membrane alterations of uninfected RBCs could be responsible for spreading of infection and RBC lysis during infection.
Sabolovic
D
D
INSERM Unit 313, Institute of Health & Development, Paris, France.
Bouanga
J C
JC
Danis
M
M
Mazier
D
D
Gentilini
M
M
D000328:Adult; D000818:Animals; D004563:Electrochemistry; D004586:Electrophoresis; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008288:Malaria / Q000097:blood* / Q000469:parasitology; D016778:Malaria, Falciparum / Q000097:blood / Q000469:parasitology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D010967:Plasmodium yoelii
D008041:Linoleic Acids; D019787:Linoleic Acid