Determination of aflatoxins in food by use of an automatic work station.
An automated HPLC method with postcolumn derivatization is described for the determination of aflatoxins B1, B2, G1 and G2 after an immunoaffinity column clean-up. It can be used for the determination of aflatoxins in a variety of foodstuffs such as nuts, nut-like products (pistachios, almonds, etc.) and dried fruit. The aflatoxins are extracted with methanol-water, followed by a filtration step. Dilution of the extract, mixing, immunoaffinity column clean-up, elution of the aflatoxins and optional on-line HPLC are performed by an automatic work station (Zymark BenchMate). The subsequent HPLC analysis includes a postcolumn derivatization step with iodine solution and fluorimetric detection. The method compared well with manual techniques and another automated method.
Niedwetzki
G
G
Dr. Wiertz-Dipl.-Chem. Eggert-Dr. Jörissen GmbH, Handels- und Umweltschutzlaboratorium, Hamburg, Germany.
Lach
G
G
Geschwill
K
K
D000348:Aflatoxins / Q000032:analysis*; D001331:Automation; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*
D000348:Aflatoxins