Analysis of type II polyketide beta-ketoacyl synthase specificity in Streptomyces coelicolor A3(2) by trans complementation of actinorhodin synthase mutants.
Complementation of defined actinorhodin beta-ketoacyl synthase (KS) mutants by various other KS genes suggested that the ORF1-encoded KS may be relatively generalized in function, whereas the ORF2-encoded KS component may provide specificity in polyketide chain construction. Evidence for differential temporal-spatial expression of the actinorhodin and spore pigment KSs in Streptomyces coelicolor was obtained.
Kim
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Department of Microbiology, University of Minnesota, St. Paul 55108.
Hopwood
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Sherman
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D000818:Animals; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology*; D005816:Genetic Complementation Test; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D009154:Mutation / Q000502:physiology; D016366:Open Reading Frames / Q000235:genetics / Q000502:physiology*; D051381:Rats; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D014170:Transformation, Genetic
D009097:Multienzyme Complexes