IL-1 inducible genes in human umbilical vein endothelial cells.
In an attempt to understand more directly the molecular mechanisms involved in the cellular response of endothelial cells to Interleukin-1 (IL-1), we have made several cDNA libraries from human umbilical vein endothelial cells (HUVEC) stimulated for 1 h with IL-1 in the presence of cycloheximide. The cDNA libraries were differentially screened with labelled cDNA derived from mRNA isolated from untreated or IL-1 treated HUVEC. Forty cDNA clones induced by IL-1 were isolated and partially sequenced. Thirty-eight of these corresponded to known genes, that is IL-8, ELAM-1, GRO-alpha, GRO-beta, PA-I and I-kB. The last two clones contained an identical insert belonging to a previously unknown gene. The full length cDNA of this new gene was isolated and called PTX3. It encodes for a 42 kD, 381 amino-acid long protein which shows in its 3' half a high degree of homology to all the known members of the pentaxin gene family, including C-reactive protein (CRP) and serum amyloid P component (SAP). PTX3 may represent a novel marker of inflammatory reactions, particularly those involving the vessel wall.
Introna
M
M
Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
Breviario
F
F
d'Aniello
E M
EM
Golay
J
J
Dejana
E
E
Mantovani
A
A
D002097:C-Reactive Protein; D004730:Endothelium, Vascular / Q000378:metabolism*; D005786:Gene Expression Regulation; D017781:Genes, Immediate-Early; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D000683:Serum Amyloid P-Component / Q000235:genetics; D014471:Umbilical Veins / Q000378:metabolism*; D000099153:Pentraxins
D002097:C-Reactive Protein; D007375:Interleukin-1; D000683:Serum Amyloid P-Component; D000099153:Pentraxins