Reconstruction of glucose uptake and phosphorylation in a glucose-negative mutant of Escherichia coli by using Zymomonas mobilis genes encoding the glucose facilitator protein and glucokinase.
Expression of the Zymomonas mobilis glf (glucose facilitator protein) and glk (glucokinase) genes in Escherichia coli ZSC113 (glucose negative) provided a new functional pathway for glucose uptake and phosphorylation. Both genes were essential for the restoration of growth in glucose minimal medium and for acid production on glucose-MacConkey agar plates.
Snoep
J L
JL
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
Arfman
N
N
Yomano
L P
LP
Fliege
R K
RK
Conway
T
T
Ingram
L O
LO
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D005941:Glucokinase / Q000235:genetics*; D005947:Glucose / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010766:Phosphorylation; D017355:Zymomonas / Q000235:genetics*
D001426:Bacterial Proteins; C086512:glucose facilitator protein, Zymomonas; D005941:Glucokinase; D005947:Glucose