A canine model of acute coronary artery thrombosis for the evaluation of reperfusion strategies.
A model of coronary artery thrombosis which: (1) provides a stable thrombus; (2) incorporates intimal injury; (3) has a low mortality rate; (4) responds predictably to thrombolytic therapy, and (5) is technically simple, was developed. Intimal injury was produced proximal to a critical stenosis and followed by the infusion of a blood and thrombin mixture into the injured segment. After thrombus formation flow remained absent in all control animals (n = 7). Microscopy showed intimal injury and coronary thrombosis with platelets adherent to the subendothelium. In animals treated with tissue plasminogen activator (n = 7) flow returned to > 60% of baseline at 20.2 +/- 7.7 min and was cyclical. Mortality and complications were infrequent. This model is useful in investigations of reperfusion therapy.
Gurbel
P A
PA
Department of Medicine, University of Maryland Medical Center, Baltimore 21201.
MacCord
C S
CS
Anderson
R D
RD
Scott
H
H
Atar
D
D
Mergner
W
W
Herzog
W R
WR
D000818:Animals; D001792:Blood Platelets / Q000473:pathology; D003328:Coronary Thrombosis / Q000473:pathology / Q000628:therapy*; D003331:Coronary Vessels / Q000473:pathology; D004195:Disease Models, Animal; D004285:Dogs; D004730:Endothelium, Vascular / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D015912:Thrombolytic Therapy; D010959:Tissue Plasminogen Activator / Q000008:administration & dosage*
D010959:Tissue Plasminogen Activator