[Optimization of determining human erythrocyte glutathione reductase activity using a semiautomatic analyzer].
A relatively simple procedure was developed for estimation of the glutathione reductase activity using half-automatic analyzer. The procedure involved: preparation of hemolysates with minimal rate of erythrocyte destruction; substitution of buffers with high ionic strength for 0.05 M Tris-HCl buffer not containing EDTA. Most effective period of preincubation was found; dependence of the reaction rate on FAD was studied. The procedure developed was approved using two types of half-automatic analyzer and the model FP-901 was shown to be the most suitable for estimation of the glutathione reductase holoenzyme activity.
Mal'tsev
G Iu
GIu
Orlova
L A
LA
D001331:Automation; D002021:Buffers; D004912:Erythrocytes / Q000201:enzymology*; D005980:Glutathione Reductase / Q000097:blood*; D006801:Humans; D013053:Spectrophotometry / Q000295:instrumentation
D002021:Buffers; D005980:Glutathione Reductase
Optimizatsiia opredeleniia aktivnosti glutationreduktazy éritrotsitov cheloveka na poluavtomaticheskom analizatore.