Targeting frequency for deletion vectors in embryonic stem cells.
We analyzed the gene targeting frequencies and recombination products generated by a series of replacement deletion vectors which target the hprt (hypoxanthine phosphoribosyltransferase) locus in mouse embryonic stem cells. We found that the targeting frequency of a 19.2-kb deletion was comparable to that of a 3-kb deletion or a conventional replacement event in which a 1.7-kb fragment was inserted into the locus. We also observed different integration patterns for these deletion vectors. A result of this finding is that a wide range of genomic deletions in embryonic stem cells is feasible.
Zhang
H
H
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
Hasty
P
P
Bradley
A
A
D000818:Animals; D015139:Blotting, Southern; D002872:Chromosome Deletion; D004247:DNA / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D004622:Embryo, Mammalian; D005822:Genetic Vectors; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D051379:Mice; D015183:Restriction Mapping; D017384:Sequence Deletion; D013234:Stem Cells; D014162:Transfection / Q000379:methods*
D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase