title: Identification and characterization of genes encoding polycyclic aromatic hydrocarbon dioxygenase and polycyclic aromatic hydrocarbon dihydrodiol dehydrogenase in Pseudomonas putida OUS82.
abstract: Naphthalene and phenanthrene are transformed by enzymes encoded by the pah gene cluster of Pseudomonas putida OUS82. The pahA and pahB genes, which encode the first and second enzymes, dioxygenase and cis-dihydrodiol dehydrogenase, respectively, were identified and sequenced. The DNA sequences showed that pahA and pahB were clustered and that pahA consisted of four cistrons, pahAa, pahAb, pahAc, and pahAd, which encode ferredoxin reductase, ferredoxin, and two subunits of the iron-sulfur protein, respectively.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Okayama University of Science, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takizawa
authors.forename: N
authors.initials: N
authors.lastname: Kaida
authors.forename: S
authors.initials: S
authors.lastname: Torigoe
authors.forename: T
authors.initials: T
authors.lastname: Moritani
authors.forename: T
authors.initials: T
authors.lastname: Sawada
authors.forename: S
authors.initials: S
authors.lastname: Satoh
authors.forename: H
authors.initials: H
authors.lastname: Kiyohara
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D049308:Dioxygenases; D005798:Genes, Bacterial / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics* / Q000502:physiology; D009281:Naphthalenes / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009281:Naphthalenes; C031721:naphthalene; D010105:Oxygenases; D049308:Dioxygenases; C086736:pahA protein, Pseudomonas putida