Isolation of a carp complement protein homologous to mammalian factor D.
Factor D of carp (Cyprinus carpio) complement was purified to apparent homogeneity by a 4-step chromatographic procedure and examined for physicochemical and functional properties. Carp factor D proved to be an alpha-globulin with a molecular mass of 29 kDa and the serum concentration was estimated to be 6 micrograms/ml. The NH2-terminal amino acid sequence (30 residues including five unidentified positions) of carp factor D showed high homologies (57-60%) to those of mammalian factor D. Neither functional compatibility nor common antigenicity was observed between carp and human factor D. This report is apparently the first description on the factor D molecule in a non-mammalian species.
Yano
T
T
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Nakao
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000276:immunology*; D011416:Complement Factor D / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data
C493488:CFD protein, human; D011416:Complement Factor D