title: Biphasic brown fat temperature responses to hypothalamic stimulation in rats.
abstract: Low-level electrical stimulation (monophasic square-wave pulses: 15 Hz, 7.0 microA, 0.5 ms) of the ventromedial hypothalamus (VMH) in anesthetized rats produced a decrease (phase 1) in interscapular brown adipose tissue (IBAT) temperature that was sustained for as long as the stimulus was applied (2-45 min). A rise in IBAT temperature (phase 2) occurred only after the stimulation had stopped. VMH stimulations ipsilateral and contralateral to a lateral hypothalamic (LH) lesion indicated that the phase 1 response required an intact LH, and denervation of IBAT showed that both phases required an intact sympathetic innervation. Central intracerebroventricular injections of amphetamine and dopamine produced decreases in IBAT temperature similar in magnitude to the phase 1 response to electrical stimulation of the VMH. This, as well as the observation that pimozide blocked phase 1, suggested that dopaminergic pathways were responsible for mediating the phase 1 decrease in IBAT temperature. The peripheral mechanisms responsible for phase 1 are unknown, but a vascular component might explain the unexpected decrease in IBAT temperature seen during sustained VMH stimulation.
authors.affiliation: Department of Physiology, St. George's Hospital Medical School, London, United Kingdom.
authors.forename: A J
authors.initials: AJ
authors.lastname: Woods
authors.forename: M J
authors.initials: MJ
authors.lastname: Stock
mesh_terms: D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects; D001931:Brain Mapping; D004558:Electric Stimulation; D007839:Functional Laterality; D007026:Hypothalamic Area, Lateral / Q000502:physiology; D007031:Hypothalamus / Q000502:physiology*; D008297:Male; D010868:Pimozide / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology
chemical_list: D010868:Pimozide