Characterization of the fliL gene in the flagellar regulon of Escherichia coli and Salmonella typhimurium.
filL is a small gene of unknown function that lies within the beginning of a large flagellar operon of Salmonella typhimurium and Escherichia coli. A spontaneous fliL mutant of S. typhimurium, containing a frameshift mutation about 40% from the 3' end of the gene, was moderately motile but swarmed poorly, suggesting that FliL might be a component of the flagellar motor or switch. However, in-frame deletions of the E. coli gene, including an essentially total deletion, had little or no effect on motility or chemotaxis. Thus, FliL does not appear to have a major role in flagellar structure or function and is therefore unlikely to be a component of the motor or switch; the effect on motility caused by truncation of the gene is probably an indirect one.
Raha
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Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8114.
Sockett
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Macnab
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D001426:Bacterial Proteins / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005407:Flagella / Q000502:physiology*; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000502:physiology*; D008565:Membrane Proteins; D009154:Mutation / Q000502:physiology; D018085:Regulon / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C489554:FliL protein, E coli; D008565:Membrane Proteins; C074426:FliL protein, Bacteria; D015245:Deoxyribonuclease BamHI; C035041:endodeoxyribonuclease MluI; D015252:Deoxyribonucleases, Type II Site-Specific