[Immunohistochemical localization in the intestine of swine of the cellular and humeral components of the immune response].
By immunohistochemistry, lymphocyte subsets of the swine gut were localized either in the epithelium or in the lamina propria. In addition, the distributions of class I and class II antigens were also characterized, using the streptavidin-biotin-peroxidase complex as the revelator; the endogenous peroxidase activity was distinguished from the specific by cobalt chloride. The results show a compartmentalization in the distribution of lymphocytes: in the epithelium, all the cells were T lymphocytes, the majority of the CD8 phenotype; in contrast, in the lamina propria, T and B cells were represented in similar proportions: T cells were constituted of both CD4 and CD8 cells (with slightly more CD4 than CD8 cells), while majority of B cells harboured the IgA isotype. Class I MHC was present on epithelium cells of both the villi and crypts. In contrast MHC class II was only present on epithelial cells of the villi. Both SC and IgA were localized in the epithelium cells of the crypt. Thus, this compartmentalization suggests the possibility of an immune response in the gut.
Olivier
M
M
Equipe de Génétique et Immunité, centre de Tours, Nouzilly, France.
Berthon
P
P
Salmon
H
H
D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007150:Immunohistochemistry; D007422:Intestines / Q000166:cytology / Q000276:immunology*; D016131:Lymphocyte Subsets; D008264:Macrophages; D013552:Swine / Q000276:immunology*
D007071:Immunoglobulin A, Secretory
Localisation immunohistochimique dans l'intestin de porc des composantes celluaires et humorales de la réponse immunitaire.