Detection of antibodies in serum against M. tuberculosis using western blot technique. Comparison between sarcoidosis patients and healthy subjects.
Antibodies in serum of IgG and IgA class against Mycobacterium tuberculosis were detected by Western blot technique in 32 patients with pulmonary sarcoidosis and in 10 BCG and 10 non-BCG vaccinated healthy individuals. All three groups demonstrated multiple IgG and IgA antibodies against Mycobacterium tuberculosis protein antigens. Preabsorption of the sera with an Escherichia coli antigen did not influence the results. There were no consistent differences in the antibody profiles in patients with sarcoidosis and healthy subjects.
Milman
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Department of Pulmonary Medicine, Gentofte Hospital, Copenhagen, Denmark.
Andersen
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AB
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis*; D001500:BCG Vaccine; D015153:Blotting, Western; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D017565:Sarcoidosis, Pulmonary / Q000276:immunology / Q000382:microbiology*; D014376:Tuberculosis / Q000517:prevention & control
D000907:Antibodies, Bacterial; D001500:BCG Vaccine; D007070:Immunoglobulin A; D007074:Immunoglobulin G