Bovine testis acylphosphatase: purification and amino acid sequence.
Two acylphosphatase molecular forms have been isolated from bovine testis. Their amino acid sequence was determined. One (ACY1) consists of 98 amino acid residues, while the other one (ACY2) consists of 100 amino acid residues. Both molecular forms are N-acetylated and differ only in the amino terminus. ACY2 has an additional Ser-Met tail with respect to ACY1. Both ACY1 and ACY2 are organ-common type isoenzymes and thus differ for about half of the amino acid positions from the previously sequenced bovine muscle isoenzyme.
Pazzagli
L
L
Department of Biochemical Sciences, University of Florence, Italy.
Cappugi
G
G
Camici
G
G
Manao
G
G
Ramponi
G
G
D017766:Acid Anhydride Hydrolases / Q000737:chemistry* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002645:Chickens; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004372:Ducks; D004912:Erythrocytes / Q000201:enzymology; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D017433:Protein Structure, Secondary; D011817:Rabbits; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013552:Swine; D013737:Testis / Q000201:enzymology*; D014422:Turkeys; D000097024:Acylphosphatase
D017766:Acid Anhydride Hydrolases; D000596:Amino Acids; D000097024:Acylphosphatase