Identification of clozapine N(+)-glucuronide in the urine of patients treated with clozapine using electrospray mass spectrometry.
Clozapine N(+)-glucuronide was detected in the urine of five patients chronically treated with clozapine. Identification was made on the basis that the material isolated from urine had the same high-performance liquid chromatographic retention time and positive electrospray mass spectra as that of an authentic reference standard of clozapine N(+)-glucuronide. These results indicate that electrospray mass spectrometry is a valuable technique in the analysis of low-molecular-weight biologically derived N(+)-glucuronide metabolites.
Luo
H
H
College of Pharmacy, University of Saskatchewan, Saskatoon, Canada.
McKay
G
G
Midha
K K
KK
D000328:Adult; D003024:Clozapine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000627:therapeutic use* / Q000652:urine; D005965:Glucuronates / Q000652:urine*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged
D005965:Glucuronates; C086632:clozapine N-glucuronide; D003024:Clozapine