[Inhibition of esterase by L-lysine, the activator and fibrinolytic activity of the plasmin-streptokinase activator complex].
The effect of L-lysine on some reactions catalysed by plasmin and the plasmin-streptokinase activator complex has been studied. The constants for competitive inhibition by L-lysine of the benzyloxycarbonyl-L-lysine p-nitrophenyl ester hydrolysis by the activator (Ki 116 mM), of the plasminogen activation by the activator (Ki 8 mM) and of fibrinolysis by plasmin (Ki 3 mM) were determined. It was found that L-lysine at concentrations below 0.05 M, which do not affect the activator's esterase activity, does inhibit fibrinolysis by plasmin and the activator complex. The effect of L-lysine on fibrinolysis under the action of the activator is complex: it inhibits both the activation of clot-entrapped plasminogen by the activator and lysis of fibrin by the plasmin formed. This inhibitory action of L-lysine is largely related to the fact that it lowers the sorption of the activator, plasminogen and plasmin on fibrin, competing with fibrin for their lysine-binding sites as well as worsens the activator-plasminogen binding.
Aĭsina
R B
RB
Gaĭsarian
E S
ES
Snitko
Ia E
IaE
Varfolomeev
S D
SD
D001665:Binding Sites; D004789:Enzyme Activation; D004950:Esterases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005341:Fibrinolysin / Q000378:metabolism* / Q000494:pharmacology; D005342:Fibrinolysis / Q000187:drug effects*; D006801:Humans; D006868:Hydrolysis; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D010958:Plasminogen / Q000378:metabolism; D013300:Streptokinase / Q000378:metabolism* / Q000494:pharmacology
C034117:N-benzyloxycarbonyllysine 4-nitrophenyl ester; D010958:Plasminogen; D004950:Esterases; D013300:Streptokinase; D005341:Fibrinolysin; D008239:Lysine
Ingibirovanie L-lizinom ésteraznoĭ, aktivatornoĭ i fibrinoliticheskoĭ aktivnosti aktivatornogo kompleksa plazmin-streptokinaza.