[HPLC determination of diprafenone and its active metabolite 5-hydroxydiprafenone in human plasma].
A simple, specific and sensitive HPLC method is described, which allows the quantitation of diprafenone and its active metabolite 5-hydroxydiprafenone in human plasma. Propafenone is used as internal standard. After extraction by diethyl ether from plasma, the analytes are reextracted with 5% phosphoric acid from the organic phase. Chromatographic separation on a RP-18 column and UV detection (220 nm) allows the sensitive determination of diparafenone and 5-hydroxydiprafenone in plasma with a lower limit of quantitation of 5.0 ng/ml for both substances. The method was successfully applied to human plasma samples from 24 subjects after oral or i.v. administration of 300 mg or 50 mg diprafenon hydrochloride, respectively.
Zhong
D
D
Zentrallaboratorium Deutscher Apotheker, Eschborn.
Blume
H
H
D000284:Administration, Oral; D000889:Anti-Arrhythmia Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007275:Injections, Intravenous; D011405:Propafenone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet
D000889:Anti-Arrhythmia Agents; C086480:5-hydroxydiprafenone; C047546:diprafenone; D011405:Propafenone
HPLC-Bestimmung von Diprafenon und seinem aktiven Metaboliten 5-Hydroxydiprafenon in Humanplasma.