Neoplastic transformation dose response of oncogene-transfected rat embryo cells by gamma rays or 6 MeV alpha particles.
We measured a dose-response relationship for induction of neoplastic transformation by 6 MeV alpha particles and 137Cs gamma rays in REC:myc and REC:ras cells, that is, rat embryo cells (REC) transfected with the c-myc or the Ha-ras oncogenes. The 6 MeV alpha particles simulated 222Rn emissions for risk assessment relative to low-LET radiations. The dose of gamma rays was approximately twice that of alpha particles for a neoplastic transformation frequency of 10(-3). The survival of the REC cells containing oncogenes was comparable to that of the commonly used C3H 10T1/2 cells for the same dose, but the former were more refractory to radiation-induced neoplastic transformation. Neoplastic transformation frequency measured in REC cells was 3 times lower than those typically measured in C3H 10T1/2 cells at a gamma-ray dose of 6 Gy, and 5-10 times lower at an alpha-particle dose of 3 Gy.
Ling
C C
CC
Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, 10021.
Weiss
H
H
Strauss
A
A
Endlich
B
B
Sheh
Y
Y
Wei
J X
JX
Orazem
J
J
D000512:Alpha Particles; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D002478:Cells, Cultured; D002588:Cesium Radioisotopes; D004307:Dose-Response Relationship, Radiation; D004622:Embryo, Mammalian; D005720:Gamma Rays; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D011519:Proto-Oncogenes; D011886:Radon; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000235:genetics
D002588:Cesium Radioisotopes; D011886:Radon