title: [Renal lithostathine: a new protein inhibitor of lithogenesis].
abstract: Lithostathine is a protein of pancreatic secretion inhibiting calcium carbonate crystal growth. Antibodies to lithostathine were used to identify a related protein in urine and kidney stones. Western blot analysis of proteins extracted from concentrated normal urine or kidney stones demonstrated the presence of a protein with an apparent molecular weight of 23 kDa. The same antibodies were used in immunolocalization experiments on fresh human nephrectomy specimens cryosections. A positive signal was observed in the cells of proximal tubules and thick ascending limbs of Henle's loop. Protein extracts of renal stones inhibited calcium carbonate crystal growth. Because of its structural and functional similarities with pancreatic lithostathine, it was called renal lithostathine.
authors.affiliation: INSERM U315, Marseille.
authors.forename: J M
authors.initials: JM
authors.lastname: Verdier
authors.forename: B
authors.initials: B
authors.lastname: Dussol
authors.forename: P
authors.initials: P
authors.lastname: Casanova
authors.forename: M
authors.initials: M
authors.lastname: Daudon
authors.forename: P
authors.initials: P
authors.lastname: Dupuy
authors.forename: P
authors.initials: P
authors.lastname: Berthezène
authors.forename: R
authors.initials: R
authors.lastname: Boistelle
authors.forename: Y
authors.initials: Y
authors.lastname: Berland
authors.forename: J C
authors.initials: JC
authors.lastname: Dagorn
mesh_terms: D000818:Animals; D002119:Calcium Carbonate / Q000737:chemistry; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000494:pharmacology / Q000652:urine; D003460:Crystallization; D006801:Humans; D007668:Kidney / Q000737:chemistry*; D007669:Kidney Calculi / Q000737:chemistry*; D051847:Lithostathine; D009419:Nerve Tissue Proteins
chemical_list: D002135:Calcium-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human; D002119:Calcium Carbonate
vernacular_title: La lithostathine rénale: un nouvel inhibiteur protéique de la lithogénèse.