Organization of the mouse GP42/Basigin gene: a member of the Ig superfamily.
We have mapped and sequenced the GP42/Basigin gene isolated from a Balb/C mouse genomic library. The genomic organization and upstream, putative regulatory, regions of this gene have not been previously reported. Our data show that exon 5 of the GP42/Basigin gene encodes the carboxy proximal half of the second Ig-like domain, the highly conserved transmembrane region and a portion of the cytoplasmic tail. This inclusion of Ig-like and other functional domains in a single exon is unusual. Splice junction analysis indicates that two reported alternate GP42/Basigin cDNA isoforms are likely due to cloning artifacts. In addition, we find that GP42/Basigin is polymorphic in mice. Our data also support the proposal that the transmembrane domain and portions of the cytoplasmic region of GP42/Basigin have been evolutionarily conserved.
Cheng
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Y
Neurology Department, University of Pennsylvania, Philadelphia 19104.
Li
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Kamholz
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J
Burns
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FR
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface / Q000235:genetics*; D030161:Avian Proteins; D001483:Base Sequence; D051926:Basigin; D001798:Blood Proteins; D018076:DNA, Complementary / Q000302:isolation & purification; D005091:Exons; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D016415:Sequence Alignment
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D001798:Blood Proteins; C102624:Bsg protein, Gallus gallus; C497887:Bsg protein, mouse; C079432:Bsg protein, rat; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D051926:Basigin