title: Assessment of brain muscarinic acetylcholinergic receptors in living mice using a simple probe, [125I]-4-iododexetimide and [125I]-4-iodolevetimide.
abstract: This study describes assessment of brain muscarinic acetylcholinergic receptors in living mice using a single-crystal radiation detection system, the high-affinity antagonist [125I]-4-iododexetimide, and the inactive enantiomer [125I]-4-iodolevetimide. Kinetics of radioligand binding, as well as perturbation by atropine displacement, can be determined using this simple probe technique.
authors.affiliation: Department of Environmental Health Sciences, Johns Hopkins Medical Institutions, Baltimore, MD 21205-2179.
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
authors.forename: H W
authors.initials: HW
authors.lastname: Müller-Gärtner
authors.forename: J R
authors.initials: JR
authors.lastname: Lever
authors.forename: H T
authors.initials: HT
authors.lastname: Ravert
authors.forename: R F
authors.initials: RF
authors.lastname: Dannals
authors.forename: T R
authors.initials: TR
authors.lastname: Guilarte
authors.forename: H N
authors.initials: HN
authors.lastname: Wagner
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D003909:Dexetimide / Q000031:analogs & derivatives* / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011869:Radioligand Assay; D011976:Receptors, Muscarinic / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D007457:Iodine Radioisotopes; D011976:Receptors, Muscarinic; C066812:4-iododexetimide; D003909:Dexetimide; D001285:Atropine