title: [Prostaglandin D2-synthase from cattle brain: purification and physico-chemical properties].
abstract: Prostaglandin D2-synthase has been purified from bovine brain for the first time and characterized. The enzyme has a molecular mass of 21 kDa and a pH optimum at 9.0 and is activated by dithiothreitol. The presence in the enzyme molecule of carbohydrates and lipids has been established and a preliminary conclusion made about the qualitative composition of the lipids. The ability of the enzyme to form aggregates under the influence of the detergent has been demonstrated. The role of the lipid component in the preservation of the enzyme monomeric structure as well as the possible mechanisms of action of the above inhibitors and activators are discussed.
authors.forename: S A
authors.initials: SA
authors.lastname: Avakian
authors.forename: A S
authors.initials: AS
authors.lastname: Boiadzhian
authors.forename: A G
authors.initials: AG
authors.lastname: Pogosian
authors.forename: K G
authors.initials: KG
authors.lastname: Karagezian
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D006863:Hydrogen-Ion Concentration; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000302:isolation & purification* / Q000378:metabolism; D054834:Lipocalins; D008970:Molecular Weight
chemical_list: D054834:Lipocalins; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase
vernacular_title: Prostaglandin D2-sintaza iz mozga byka: ochistka i fiziko-khimicheskie svoĭstva.