Effects of jen-sheng-yang-yung-tang on cellular immunocompetence of gamma-irradiated mice.
This study was conducted to determine the therapeutic effects of Jen-Sheng-Yang-Yung-Tang on mice irradiated by gamma-ray. A total of 160 male ICR strain mice, 6-7 weeks of age, were chosen to receive different treatment of radiation and Jen-Sheng-Yang-Yung-Tang. After the treatments, six to eight mice from each group were sacrificed on days 1, 5, 12, 19, 26 and 33. The body and splenic weights of mice by different treatments were measured and the splenic cells were separated under aseptic conditions, thereafter. The changes of cellular immunocompetence in mice following treatments were measured by 3H-thymidine incorporation in each experimental mouse. The results revealed that 4 Gy of gamma-ray irradiation inhibited the increases of body and splenic weights and exerted an obvious inhibitory effect on the blastogenic responses of the splenic lymphoid cells after being stimulated by mitogens such as PHA, Con A, PWM and LPS. Jen-Sheng-Yang-Yung-Tang administration seemed to help the recovery of cellular immunocompetence in the gamma-ray irradiated mice. Among these treatments, Jen-Sheng-Yang-Yung-Tang administered with the concentration of 20 mg/20 g body weight after irradiation enhanced the recovery of radiation damages and had the highest efficacy of any other kinds of treatments.
Hsu
H Y
HY
School of Technology for Medical Sciences, Kaohsiung, Taiwan.
Hau
D M
DM
Lin
C C
CC
D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000528:radiation effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005720:Gamma Rays; D007121:Immunocompetence / Q000187:drug effects* / Q000528:radiation effects; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011837:Radiation-Protective Agents; C086343:jen-sheng-yang-yung-tang