A cis-acting region required for the regulated expression of grg-1, a Neurospora glucose-repressible gene. Two regulatory sites (CRE and NRS) are required to repress grg-1 expression.
Grg-1 is a Neurospora gene which was identified as being a highly expressed glucose-repressible gene. A cis-acting regulatory region required for the regulated expression of grg-1 has been characterized. The regulatory region is found between 440 and 500 nucleotides upstream of the first major grg-1 start of transcription site and contains two distinct cis-acting regulatory elements. The upstream element consists of the sequence GTGACGTCAC, which is identical to the previously identified CRE (Cyclic AMP-Responsive Element). The second element is a newly defined cis-acting regulatory site. The element has the sequence TTGCTAGCAA and has been named NRS (Neurospora Repressor Site). DNA binding proteins can be shown to bind to both of these cis-acting regulatory elements. Experiments in which these sites were deleted demonstrate that both cis-acting regulatory elements are required to turn off the in vitro expression of the grg-1 gene under conditions of glucose sufficiency.
Wang
Z
Z
Department of Biological Sciences, State University of New York at Buffalo 14260.
Deak
M
M
Free
S J
SJ
D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005800:Genes, Fungal; D005947:Glucose / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009491:Neurospora / Q000235:genetics*; D009687:Nuclear Proteins / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology*; D012097:Repressor Proteins / Q000378:metabolism
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D005947:Glucose