title: Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein.
abstract: The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47-->methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 A resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein-single-stranded DNA complex.
authors.affiliation: Life Sciences Division, Los Alamos National Laboratory, NM 87545.
authors.forename: M M
authors.initials: MM
authors.lastname: Skinner
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: D H
authors.initials: DH
authors.lastname: Leschnitzer
authors.forename: Y
authors.initials: Y
authors.lastname: Guan
authors.forename: H
authors.initials: H
authors.lastname: Bellamy
authors.forename: R M
authors.initials: RM
authors.lastname: Sweet
authors.forename: C W
authors.initials: CW
authors.lastname: Gray
authors.forename: R N
authors.initials: RN
authors.lastname: Konings
authors.forename: A H
authors.initials: AH
authors.lastname: Wang
authors.forename: T C
authors.initials: TC
authors.lastname: Terwilliger
mesh_terms: D018360:Crystallography, X-Ray; D017912:Inovirus / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D012645:Selenomethionine / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D014764:Viral Proteins; C069558:gene V protein, Enterobacteria phage f1; D012645:Selenomethionine