title: In vitro activation of rat brain protein kinase C by polyenoic very-long-chain fatty acids.
abstract: A variety of fatty acids including the cis-polyunsaturated very-long-chain fatty acids (VLCFA) (> 22 carbon atoms) common in retina, spermatozoa, and brain were examined for their ability to activate protein kinase C (PKC) purified from rat brain. Arachidonic [20:4(n-6)], eicosapentaenoic [20:5(n-3)], and docosahexaenoic [22:6(n-3)] acids as well as the VLCFA dotriacontatetraenoic [32:4(n-6)] and tetratriacontahexaenoic [34:6(n-3)] were equally capable of activating PKC in vitro with maximal activity being between 25 and 50 microM. The phorbol ester 12-O-tetradecanoylphorbol 13-acetate further enhanced the in vitro activation of PKC when added to the protein kinase assay system with the fatty acids. The fully saturated arachidic acid (20:0) was inactive in both assay systems. The potential significance of the in vitro activation of PKC by the VLCFA is discussed.
authors.affiliation: Department of Immunology and University of Adelaide, Australia.
authors.forename: S J
authors.initials: SJ
authors.lastname: Hardy
authors.forename: A
authors.initials: A
authors.lastname: Ferrante
authors.forename: B S
authors.initials: BS
authors.lastname: Robinson
authors.forename: D W
authors.initials: DW
authors.lastname: Johnson
authors.forename: A
authors.initials: A
authors.lastname: Poulos
authors.forename: K J
authors.initials: KJ
authors.lastname: Clark
authors.forename: A W
authors.initials: AW
authors.lastname: Murray
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001921:Brain / Q000201:enzymology*; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005227:Fatty Acids / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D006801:Humans; D008297:Male; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; C086312:tetratriacontahexaenoic acid; C086311:dotriacontatetraenoic acid; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate