Cytogenetic triclonality in acute myeloid leukemia: a morphologic, immunologic and in situ hybridization study.
Cytogenetically unrelated clones are uncommon in hematologic malignancies. We report a case of acute myeloid leukemia, which consisted morphologically of two populations of small and large blasts demonstrating immunophenotypic heterogeneity. Cytogenetic analysis showed 3 karyotypically abnormal clones: 47,XY, +14/45,XY,dic(5;17)(q11;p11),14dmin, and a near-tetraploid clone. In situ hybridization showed that the near-tetraploid clone corresponded to the large blasts, and the near-diploid clones the small blasts, therefore demonstrating a direct relationship between cell size and DNA content. The diverse morphologic, immunologic and cytogenetic heterogeneity observed in our case suggested hematopoietic oligoclonality.
Kwong
Y L
YL
University Department of Medicine, Queen Mary Hospital, Hong Kong.
Lam
C K
CK
Chan
A Y
AY
Lie
A K
AK
Chan
L C
LC
D000368:Aged; D002869:Chromosome Aberrations; D006801:Humans; D016130:Immunophenotyping; D017403:In Situ Hybridization; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male