Aromatic substrate molecules bind at the distal heme pocket of myeloperoxidase.
Binding of aromatic substrate molecules to myeloperoxidase has been investigated by EPR spectroscopy and model building. Binding of aromatic substrate molecules, such as phenol, p-cresol, resorcinol, and 4-amino salicylate, replaces the original rhombic high spin EPR spectrum of the ferric enzyme (g = 6.74, 5.18, and 1.97) by another high spin signal (g = 7.04, 4.87, and 1.93) indicating that these substrate molecules bind near the heme center of the enzyme. Salicylhydroxamic acid and benzohydroxamic acid complexes of myeloperoxidase showed EPR spectra composed of high spin (g = 6.99, 4.93, and 1.95) and low spin (2.66, 2.22, and 1.81) signals. The hydroxamic side chains of these two substrates seem to interact with the heme iron. Model building based on the three-dimensional structure of the enzyme (Zeng, J., and Fenna, R. E. (1992) J. Mol. Biol. 226, 185-207) revealed the presence of a hydrophobic pocket at the entrance of the distal heme cavity where the aromatic ring of these substrates can bind. Moreover, the six-membered ring portion of salicylhydroxamic acid and benzohydroxamic acid could bind to this hydrophobic pocket with the hydroxamic side chain placed between the imidazole of the distal His and the heme iron. The EPR results on lactoperoxidase and intestinal peroxidase also suggest the presence of an aromatic substrate binding site similar to that of myeloperoxidase.
Hori
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Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4970.
Fenna
R E
RE
Kimura
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Ikeda-Saito
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M
D010131:Aminosalicylic Acid / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003198:Computer Simulation; D003408:Cresols / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006418:Heme / Q000378:metabolism*; D008958:Models, Molecular; D009195:Peroxidase / Q000737:chemistry* / Q000378:metabolism*; D019800:Phenol; D010636:Phenols / Q000378:metabolism; D011487:Protein Conformation; D012118:Resorcinols / Q000378:metabolism; D014961:X-Ray Diffraction
D003408:Cresols; D010636:Phenols; D012118:Resorcinols; D019800:Phenol; D006418:Heme; D010131:Aminosalicylic Acid; D009195:Peroxidase; C077977:cresol; C031389:resorcinol