Detection by reversed-phase HPLC of trace amounts of the alpha 113His globin chain in a sheep homozygous for a quadruple alpha-globin gene chromosome.
1. In sheep alpha alpha 113His and alpha D alpha alpha 113His globin gene haplotypes, the percentage gene efficiencies, from the 5' to the 3' end, are about 32-18 and 30-14-6, respectively. 2. We previously found that in a alpha alpha alpha alpha homozygote there may be a 1-2% alpha 113His chains, which however were difficult to resolve from alpha 113Leu chains by CMC chromatography. 3. We report here the experimental conditions which allowed a neat resolution of the 1% alpha 113His chain peak by RP-HPLC. 4. This finding strongly suggests the occurrence of the alpha D alpha alpha alpha 113His haplotype where the percentage gene efficiencies are 30-(14-5)-1, supporting the existence of a gradient of decreasing expression in multiple alpha-globin gene haplotypes.
Vestri
R
R
Istituto di Fisiologia Umana, Università Cattolica S. Cuore, Roma, Italy.
Pieragostini
E
E
Manca
L
L
Masala
B
B
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002874:Chromosome Mapping; D005838:Genotype; D005914:Globins / Q000032:analysis* / Q000235:genetics; D006239:Haplotypes; D006639:Histidine; D006720:Homozygote; D012756:Sheep
D006639:Histidine; D005914:Globins