Comparison of polymerase chain reaction and conventional culture for the detection of legionellas in hospital water samples.
A detection system for Legionella spp. based on the polymerase chain reaction (PCR) was used to assess the diagnostic value of PCR for the surveillance of contamination of man-made water systems by legionellas. A previously-published primer system was chosen to amplify a fragment of the 5S-ribosomal gene of Legionella spp. A total of 78 water samples from various sources were examined by PCR and culture on MWY Legionella selective agar. Fifty-seven of 78 water samples were positive by both test systems (73%), nine were positive by PCR only (11.5%), another nine were positive by culture but negative by PCR (11.5%), and three were negative by both techniques (3.8%). The PCR was inhibited when large amounts of rust were present in the samples. Culture failed to detect legionellas in samples that contained large numbers of other bacteria capable of overgrowing the legionellas. These results show that PCR is a rapid and sensitive technique for the detection of legionella contamination in water samples and that PCR and culture complement each other in monitoring of environmental water samples.
Maiwald
M
M
Hygiene-Institut der Universität, Abt. Hygiene und medizinische Mikrobiologie, Heidelberg, Germany.
Kissel
K
K
Srimuang
S
S
von Knebel Doeberitz
M
M
Sonntag
H G
HG
D001483:Base Sequence; D015169:Colony Count, Microbial; D006761:Hospitals; D006358:Hot Temperature; D007875:Legionella / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013242:Sterilization; D014871:Water Microbiology; D014874:Water Pollutants, Chemical; D014881:Water Supply
D014874:Water Pollutants, Chemical