Effects of nerve growth factor and dexamethasone on Na+,K(+)-ATPase of cultured PC12h cells.
When PC12h cells were cultured for 4 days in the presence of 50 ng/ml of nerve growth factor (NGF), they showed elongated dendrites and specifically increased Na+,K(+)-ATPase activity. Either singly or in combination with NGF, dexamethasone also increased the specific activity of this enzyme. Western blot analysis using anti-alpha 1 and anti-alpha 2 antisera showed that PC12h cells, either before or after hormone treatment, contained the alpha 1 isoform but not the alpha 2 one. We conclude, therefore, that NGF induces Na+,K(+)-ATPase concomitantly with neuronal differentiation in PC12h cells but that the growth factor does not induce formation of the myelin sheath, which normally expresses the alpha 2 isoform of Na+,K(+)-ATPase.
Kurihara
K
K
Department of Oral Physiology, Meikai University School of Dentistry, Saitama, Japan.
Hosoi
K
K
Ueha
T
T
Nakanishi
N
N
Yamada
S
S
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D003712:Dendrites / Q000648:ultrastructure; D003907:Dexamethasone / Q000494:pharmacology*; D004790:Enzyme Induction; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells / Q000201:enzymology* / Q000648:ultrastructure; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D009414:Nerve Growth Factors; D003907:Dexamethasone; D000254:Sodium-Potassium-Exchanging ATPase