Anti-idiotypes against an anti-haloperidol antibody bind to sigma receptors.
Anti-idiotypic monoclonal antibodies that interact with the binding site of sigma receptors were generated. First, BALB/c mice were immunized with a haloperidol-bovine serum albumin conjugate, and monoclonal anti-haloperidol antibodies that recognize the piperidinyl moiety of haloperidol molecule were obtained. Second, for generation of anti-idiotypic antibodies, BALB/c mice were immunized with the anti-haloperidol monoclonal antibodies coupled to keyhole limpet hemocyanin. Anti-idiotypic antisera and three hybridomas secreting anti-idiotypic monoclonal antibodies were obtained. All of them were shown to inhibit [3H]haloperidol binding to the anti-haloperidol antibodies. The anti-idiotypes were potent in displacing the binding of [3H]haloperidol to rat brain sigma receptors. Furthermore, they significantly immunoprecipitated the sigma receptors from a detergent-solubilized preparation. These findings demonstrate the generation of anti-idiotypic monoclonal antibodies specifically interacting with membrane-bound and solubilized sigma receptors.
Masuzawa
N
N
Department of Psychiatry, Niigata University School of Medicine, Japan.
Togashi
S
S
Itoh
N
N
Kobayashi
T
T
Inazuki
G
G
Fujimaki
M
M
Nakamura
H
H
Nakayama
H
H
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001921:Brain / Q000378:metabolism; D002417:Cattle; D006220:Haloperidol / Q000276:immunology* / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D051381:Rats; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000276:immunology / Q000378:metabolism; D017480:Receptors, sigma / Q000276:immunology* / Q000378:metabolism; D013134:Spiperone / Q000276:immunology / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007106:Immune Sera; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D017448:Receptors, Dopamine D2; D017480:Receptors, sigma; D013134:Spiperone; D006220:Haloperidol