[Expression of interferon alpha receptors in cell lines from human tumors. Relationship with the antiproliferative effect].
Alpha Interferon (IFN) membrane receptor expression was studied in four human tumor cell lines from several origins: Burkitt's lymphoma (Daudi cells), cervix adenocarcinoma (HeLa cells), larynx carcinoma (HEp-2 cells) and a grade III-IV glioblastoma (GL-5 cells). The number of receptors per cell expressed in each case did not correlate with the sensitivity of the cytostatic effect of IFN alpha-2b. However, the Scatchard analysis of the affinity of the IFN-receptor complex did correlate with this effect. Daudi cells, which expressed 1927 receptors/cells with a high affinity (Kd = 3.30 x 10(-10) M) were the most sensitive to the antiproliferative effect. Growth inhibition was 30% with only 1.6pg/ml of IFN. HeLa cells expressed a mixture of high (Kd = 3.36 x 10(-10) M) and low (Kd = 3.21 x 10(-9) M) affinity binding sites and were also sensitive to the antiproliferative effect but less than Daudi. HEp-2 and GL-5 cells, on the contrary, were very little sensitive to the antiproliferative effect of IFN and only expressed low affinity binding sites (Kd = 1.20 x 10(-8) M and Kd = 8.33 x 10(-9) M) respectively. On the other hand, IFN alpha-2b binding assays to peripheral blood lymphocytes from 8 healthy individuals were also carried out as normal cells for comparison. The values obtained were 433 +/- 166 receptors per cell and Kd = 3.68 2.66 x 10(-10) M.(ABSTRACT TRUNCATED AT 250 WORDS)
Perea
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Centro de Investigaciones Biológicas, La Habana, Cuba.
Gerónimo
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López Saura
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D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D001665:Binding Sites; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006367:HeLa Cells; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D017471:Receptors, Interferon / Q000378:metabolism*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D017471:Receptors, Interferon; D011994:Recombinant Proteins
Expresión de receptores para el interferón alfa en líneas celulares procedentes de tumores humanos. Relación con el efecto antiproliferativo.