title: Catabolite repression of the Bacillus subtilis hut operon requires a cis-acting site located downstream of the transcription initiation site.
abstract: Expression of the Bacillus subtilis hut operon is subject to regulation by catabolite repression. A set of hut-lacZ transcriptional fusions was constructed and used to identify two cis-acting sites involved in catabolite repression. The hutOCR1 operator site lies immediately downstream of the hut promoter and weakly regulates hut expression in response to catabolite repression. The downstream hutOCR2 operator site lies within the hutP gene, between positions +203 and +216, and is required for wild-type levels of catabolite repression. Both the hutOCR1 and hutOCR2 operators have sequence similarity to the sites which mediate catabolite repression of several other B. subtilis genes. Two mutations which relieve catabolite repression of hut expression were found to alter the nucleotide sequence of the hutOCR2 operator. Catabolite repression of hut expression was partially relieved in strains containing the ccpA mutation but not in strains containing either the pai or hpr mutation.
authors.affiliation: Department of Microbiology, Boston University School of Medicine, Massachusetts 02118.
authors.forename: L V
authors.initials: LV
authors.lastname: Wray
authors.forename: F K
authors.initials: FK
authors.lastname: Pettengill
authors.forename: S H
authors.initials: SH
authors.lastname: Fisher
mesh_terms: D019747:Aldose-Ketose Isomerases; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002238:Carbohydrate Epimerases / Q000235:genetics; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D004794:Enzyme Repression; D015964:Gene Expression Regulation, Bacterial; D006639:Histidine / Q000378:metabolism*; D006638:Histidine Ammonia-Lyase / Q000096:biosynthesis; D007209:Indole-3-Glycerol-Phosphate Synthase / Q000235:genetics; D007763:Lac Operon; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics; D009875:Operator Regions, Genetic / Q000235:genetics; D009876:Operon / Q000235:genetics*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics; D017354:Point Mutation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C069286:catabolite control proteins, bacteria; C052909:phosphoribosylanthranilate isomerase-indoleglycerol-phosphate synthase; D006639:Histidine; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; D007209:Indole-3-Glycerol-Phosphate Synthase; D006638:Histidine Ammonia-Lyase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases