title: Fat-storing cell abnormalities associated with endothelial cell damage after cold ischemic storage of rat liver in UW solution.
abstract: Rat liver was stored at 1 degree C in University of Wisconsin solution, and morphological changes were observed after 12, 18, 24, and 36 hr by transmission electron microscopy. There were two types of endothelial cell damage in the hepatic sinusoids. One was disruption of the endothelial linings, and the other detachment of endothelial cells into the sinusoidal space accompanied by fat-storing cell abnormalities. The former damage was seen after storage longer than 12 hr, while the latter developed after 18 hr even in the hepatic sinusoids with no disruption of the linings. Considering that fat-storing cell damage can produce endothelial cell destruction, this damage should be given attention as one of factors of endothelial cell destruction in the hepatic sinusoids after cold storage of the liver.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ohno
authors.forename: S
authors.initials: S
authors.lastname: Mochida
authors.forename: M
authors.initials: M
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Hirata
authors.forename: K
authors.initials: K
authors.lastname: Fujiwara
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003080:Cold Temperature; D004727:Endothelium / Q000648:ultrastructure; D005978:Glutathione; D007328:Insulin; D008099:Liver / Q000648:ultrastructure*; D016031:Liver Transplantation; D008297:Male; D008854:Microscopy, Electron; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D013997:Time Factors; D014021:Tissue Preservation
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose