Changes in the glycoforms of rat alpha-1-acid glycoprotein during experimental polyarthritis.
We analyzed the carbohydrate moiety of purified alpha-1-acid glycoprotein (AGP) from Lewis adult male rats that were healthy (AGPh) or had experimental polyarthritis (AGPi). Sodium dodecyl sulfate polyacrylamide gel electrophoresis before and after N-glycanase treatment showed that AGPi had a slightly lower molecular mass (43 kDa vs. 45 kDa for AGPh) due to a lesser carbohydrate content. Carbohydrate analysis of purified AGP showed a slight decrease in the sialyl and galactosyl molar ratio in polyarthritis. However, the same difference in AGPh and AGPi (i.e. 0.6 residue) between the sialyl and galactosyl molar ratio indicated more than one sialyl residue per complex-type branch. Affinity for concanavalin A (ConA) of the whole glycoprotein and released oligosaccharides showed a progression during polyarthritis towards more reactive glycoforms or more ConA-bound oligosaccharides. Anion-exchange HPLC of the ConA-fractionated oligosaccharides corroborated the decreased sialylation in polyarthritis. Taken together, these results suggest a fall in branched and sialylated oligosaccharides during experimental polyarthritis. These structural changes might be related to an increase in Gal beta 1-4GlcNAc alpha 2-6 sialyltransferase activity described elsewhere in inflammatory states.
Venembre
P C
PC
Laboratoire de Biochimie Générale, UER des Sciences Pharmaceutiques et Biologiques, Châtenay-Malabry, France.
Nguyen
C H
CH
Biou
D R
DR
Durand
G M
GM
D000818:Animals; D000838:Anions; D001169:Arthritis, Experimental / Q000097:blood* / Q000150:complications; D002241:Carbohydrates / Q000032:analysis / Q000097:blood; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003208:Concanavalin A; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D007249:Inflammation / Q000097:blood / Q000209:etiology; D007536:Isomerism; D008297:Male; D009844:Oligosaccharides / Q000032:analysis / Q000097:blood; D009961:Orosomucoid / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew
D000838:Anions; D002241:Carbohydrates; D009844:Oligosaccharides; D009961:Orosomucoid; D003208:Concanavalin A