The attachment of mineralised tissues to coverslips for observing dynamic events by confocal microscopy.
In confocal scanning optical fluorescence microscopy, using high-aperture oil immersion lenses, the best images are obtained for focus planes immediately under the coverslip, or under a continuation of the coverslip with a medium with the same, high refractive index. Therefore placing the coverslip with a layer of oil underneath it is an advantage if the sample will allow it. If experimental manipulations that can displace the coverslip are involved,--for example, attempts to make dynamic observation of fluid flow--the coverslip is best cemented to the sample with a high-index material. This report describes the use of adhesive systems developed for restorative dentistry to achieve a durable attachment of dental tissues to microscope coverslips. The technique described in this paper has been used for monitoring real-time fluid movement in dentine. The samples were examined with a high-frame-rate confocal microscope (a tandem scanning microscope). The adhesive technology also could be utilised in the microscopic preparation of other porous translucent materials.
Griffiths
B M
BM
Eastman Dental Hospital, England, UK.
Watson
T F
TF
D003804:Dentin / Q000648:ultrastructure; D017220:Dentin-Bonding Agents; D006801:Humans; D008853:Microscopy / Q000379:methods*
D017220:Dentin-Bonding Agents