Induction of B cell and T cell tolerance in vivo by anti-CD23 mAb.
T cell tolerance can be induced by B cell presentation of Ags to naive T cells. To further characterize this mechanism of T cell tolerance induction, we have investigated the effects of injecting mice with an intact rat IgG2a Ab, which binds to the B cell low-affinity Fc epsilon receptor (CD23), on the responsiveness of B cells and T cells to rat IgG2a. Our observations indicate that 1) intravenous, subcutaneous, or intraperitoneal injection of this Ab induces antigen-specific B cell and T cell tolerance; 2) both forms of tolerance are induced more completely by injection of rat IgG2a anti-CD23 mAb than by injection of an equal dose of a control rat IgG2a Ig; and 3) reduced responsiveness to Ag is seen as early as 1 to 3 days after anti-CD23 mAb injection and reaches maximum levels by 7 days after injection. Although tolerance induced by the injection of soluble proteins has been reported to be characterized by reduced production of IL-2 and IFN-gamma, but normal production of IL-4, injection of mice with rat IgG2a anti-mouse CD23 mAb greatly decreases the IL-4 response to a rat IgG2a immunogen that normally induces a large IL-4 response.
Morris
S C
SC
Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
Lees
A
A
Holmes
J M
JM
Jeffries
R D
RD
Finkelman
F D
FD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D007108:Immune Tolerance; D015847:Interleukin-4 / Q000037:antagonists & inhibitors; D051379:Mice; D008807:Mice, Inbred BALB C; D017455:Receptors, IgE / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017455:Receptors, IgE; D015847:Interleukin-4