Isolation of promoter for cytosolic phospholipase A2 (cPLA2).
Cytosolic phospholipase A2 (cPLA2) releases arachidonic acid from membrane phospholipids and is believed to be the rate-limiting enzyme in the arachidonic acid pathway. We report herein the isolation of a 3 kb fragment of rodent genomic DNA containing part of the first intron, the first exon and 5'-flanking sequence. The start site of transcription was mapped by 5'-rapid amplification of cDNA ends and corroborated by ribonuclease protection assay. The gene has a TATAless promoter with no classical Sp1 binding sites or initiator element. A microsatellite series of CA repeats was noted in the 5'-flanking region of both the rodent and human promoters. Deletion constructs have been analysed for luciferase activity and confirmed promoter activity.
Tay
A
A
Division of Nephrology, University of Toronto, Canada.
Maxwell
P
P
Li
Z
Z
Goldberg
H
H
Skorecki
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003600:Cytosol / Q000201:enzymology; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D005091:Exons; D007438:Introns; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D011401:Promoter Regions, Genetic; D051381:Rats; D016415:Sequence Alignment
D004247:DNA; D010741:Phospholipases A; D054467:Phospholipases A2