title: Host responses in patients with generalized refractory periodontitis.
abstract: Although patients with refractory periodontitis have been widely reported, no clear biologic profile of these patients has been noted. The purpose of the present study was to evaluate host responsiveness of a well-defined group of refractory periodontitis patients by determining the effect of a lipopolysaccharide (LPS) challenge on monocyte surface receptor density and on the release of inflammatory mediators. Venous blood was obtained from 7 refractory periodontitis, 8 stable periodontal maintenance, and 8 gingivitis patients with no evidence of periodontitis. Mononuclear cells were cultured in either control media or media treated with Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), or Salmonella typhimurium (S. typh) LPS. At 0 and 24 hours supernatants were assayed for prostaglandin-E2 (PGE2) and interleukin-1 beta (Il-1 beta) release by ELISA. Using flow cytometry the density of specific monocyte surface receptors were assayed with Mo3e and LeuM3 monoclonal antibodies (mAb); T-cell CD4/CD8 ratios were assayed with OKT-3, OKT-4, and OKT-8 mAb. After 24 hours incubation with Pg or S. typh LPS, the upregulation of the Mo3e receptor was significantly decreased for refractory periodontitis patients (P < 0.05) when compared to gingivitis and to stable maintenance patients. In refractory periodontitis patients the T-cell CD4/CD8 ratio was decreased. Upon stimulation with Pg or S. typh LPS, monocytes from stable maintenance and refractory periodontitis patients released more Il-1 beta (P < 0.05) and PGE2 (P = 0.13 and 0.15) than monocytes from gingivitis subjects.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Periodontics, University of Texas Health Science Center at San Antonio.
authors.forename: E
authors.initials: E
authors.lastname: Hernichel-Gorbach
authors.forename: K S
authors.initials: KS
authors.lastname: Kornman
authors.forename: S C
authors.initials: SC
authors.lastname: Holt
authors.forename: F
authors.initials: F
authors.lastname: Nichols
authors.forename: H
authors.initials: H
authors.lastname: Meador
authors.forename: J T
authors.initials: JT
authors.lastname: Kung
authors.forename: C A
authors.initials: CA
authors.lastname: Thomas
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D016516:CD4-CD8 Ratio; D016009:Chi-Square Distribution; D002908:Chronic Disease; D015232:Dinoprostone / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D010518:Periodontitis / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000276:immunology; D055293:Receptors, Urokinase Plasminogen Activator; D015854:Up-Regulation
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D015232:Dinoprostone