Cholesterol in remnant-like lipoproteins in human serum using monoclonal anti apo B-100 and anti apo A-I immunoaffinity mixed gels.
We have developed a simple, rapid assay method for apo E-rich lipoproteins (d < 1.006 g/ml), using an immunoaffinity gel mixture of anti apo B-100 and apo A-I antibodies coupled to Sepharose 4B. The immunoaffinity mixed gels adsorb normal lipoproteins containing apo A-I quantitatively as well as most lipoproteins containing apo B-100. Unbound lipoproteins are quantified by assay of cholesterol. Characterization of the unbound lipoproteins of d < 1.006 g/ml (J Lipid Res 1992; 33: 369-380) has shown that they represent chylomicron and VLDL remnant-like particles (RLP). RLP-Cholesterol(C) levels in plasma have been determined in 363 male and female normolipidemic subjects (mean +/- S.D.: 72 +/- 16 mg/l) and have been found to be higher in patients with coronary heart disease and familial dysbetalipoproteinemia. Triglyceride-rich lipoproteins may well contain both atherogenic and non-atherogenic particles that can be separated by this simple immunoadsorption assay.
Nakajima
K
K
Japan Immunoresearch Laboratories, Gunma.
Saito
T
T
Tamura
A
A
Suzuki
M
M
Nakano
T
T
Adachi
M
M
Tanaka
A
A
Tada
N
N
Nakamura
H
H
Campos
E
E
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I / Q000276:immunology*; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D002784:Cholesterol / Q000097:blood*; D002846:Chromatography, Affinity; D002914:Chylomicrons / Q000097:blood; D003327:Coronary Disease / Q000097:blood; D005260:Female; D005782:Gels; D006801:Humans; D015151:Immunoblotting; D007223:Infant; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D002914:Chylomicrons; D005782:Gels; D008074:Lipoproteins; D008079:Lipoproteins, VLDL; D002784:Cholesterol