In vitro modulation of anti-DNA secreting peripheral blood mononuclear cells of lupus patients by anti-idiotypic antibody of pooled human intravenous immune globulin.
Anti-idiotypic antibody and its F(ab')2 fragments prepared from pooled normal human IgG had a partial inhibitory effect on the spontaneous in vitro secretion of anti-DNA antibodies from blood mononuclear cells of lupus patients. The inhibitory effect was specific for anti-DNA secretion as the anti-idiotype failed to inhibit the spontaneous secretion of anti-tetanus toxoid in the same culture supernatants. Non-anti-DNA IgG or its F(ab')2 fragments from pooled normal human IgG had less inhibitory effect on anti-DNA secretion than the purified anti-Id. In vitro polyclonal IgG secretion by lupus blood mononuclear cells was equally inhibited by both the anti-idiotype and the non-anti-DNA IgG of pooled normal human IgG. There was no correlation between the in vitro suppression of anti-DNA and that of total IgG or of anti-tetanus toxoid antibody. Pooled normal human IgG could have a potential beneficial effect in the therapy of systemic lupus erythematosus by inhibiting anti-DNA production via an anti-idiotypic mechanism.
Evans
M
M
Immunology Research Laboratory, St. Luke's Hospital, Kansas City, MO 64111.
Abdou
N I
NI
D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification / Q000627:therapeutic use*; D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000627:therapeutic use; D007074:Immunoglobulin G / Q000096:biosynthesis; D016756:Immunoglobulins, Intravenous / Q000302:isolation & purification / Q000627:therapeutic use; D007165:Immunosuppression Therapy / Q000379:methods; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000628:therapy*; D013745:Tetanus Toxoid / Q000037:antagonists & inhibitors / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous; D013745:Tetanus Toxoid