Effects of prostacyclin and beraprost on ciliary motility of rabbit airway epithelium.
The effects of prostacyclin and its stable analog beraprost on ciliary beat frequency (CBF) of rabbit tracheal epithelium were studied by a microphoto-oscillation technique in vitro. Addition of these drugs increased CBF in a dose-dependent fashion: the maximal increase from the baseline value and the drug concentration required to produce a half-maximal effect were 13.3 +/- 2.0% (p < 0.05) and 1.8 +/- 0.4 x 10(-7) mol/l for prostacyclin and 30.8 +/- 3.4% (p < 0.001) and 1.6 +/- 0.5 x 10(-7) mol/l for beraprost. These effects were accompanied by the increase in intracellular cyclic AMP levels. Thus, prostacyclin and beraprost may stimulate ciliary motility and, hence, mucociliary transport through the production of cyclic AMP.
Tamaoki
J
J
First Department of Medicine, Tokyo Women's Medical College, Japan.
Sakai
S
S
Chiyotani
A
A
Takeyama
K
K
Tagaya
E
E
Konno
K
K
D000818:Animals; D002923:Cilia / Q000187:drug effects / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology* / Q000627:therapeutic use; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D014132:Trachea / Q000187:drug effects*
C048081:beraprost; D011464:Epoprostenol; D000242:Cyclic AMP