title: [Preparation of a mutant human tumor necrosis factor with increased resistance to proteases].
abstract: The potential proteolysis sites of human TNF are considered. By site-directed mutagenesis the Arg-31 residue of mature TNF was substituted by Gln. The analysis of cytotoxicity of initial and mutant (R31Q) proteins on mouse L929 fibroblasts did not reveal any differences in biological activity. For the mutant protein a change in proteolysis dynamics was shown in contrast to the natural variant: mutant TNF displayed increased stability when treated with trypsin.
authors.forename: A V
authors.initials: AV
authors.lastname: Korepanova
authors.forename: A M
authors.initials: AM
authors.lastname: Eroshkin
authors.forename: V A
authors.initials: VA
authors.lastname: Ivanisenko
authors.forename: L R
authors.initials: LR
authors.lastname: Lebedev
authors.forename: N N
authors.initials: NN
authors.lastname: Mikriukov
authors.forename: N M
authors.initials: NM
authors.lastname: Pustoshilova
authors.forename: V A
authors.initials: VA
authors.lastname: Petrenko
authors.forename: A A
authors.initials: AA
authors.lastname: Il'ichev
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002470:Cell Survival; D002478:Cells, Cultured; D010450:Endopeptidases / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D017386:Sequence Homology, Amino Acid; D014409:Tumor Necrosis Factor-alpha / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D009838:Oligodeoxyribonucleotides; D014409:Tumor Necrosis Factor-alpha; D010450:Endopeptidases
vernacular_title: Poluchenie mutantnogo faktora nekroza opukholeĭ cheloveka s povyshennoĭ ustoĭchivost'iu k proteazam.