title: Cyclin E controls S phase progression and its down-regulation during Drosophila embryogenesis is required for the arrest of cell proliferation.
abstract: Most cells of the dorsal epidermis exit from the mitotic cycle after division 16 in Drosophila embryogenesis. This exit is dependent on the down-regulation of Drosophila cyclin E (DmcycE) during the final mitotic cycle. Ectopic expression of DmcycE after the final mitosis induces entry into S phase and reaccumulation of G2 cyclins and results in progression through a complete additional cell cycle. Conversely, analyses in DmcycE mutant embryos indicate that cyclin E is required for progression through S phase of the mitotic cycle. Moreover, endoreplication, which occurs in late wild-type embryos in the same pattern as DmcycE expression, is not observed in the mutant embryos. Therefore, Drosophila cyclin E, which forms a complex with the Dmcdc2c kinase, controls progression through S phase and its down-regulation limits embryonic proliferation.
authors.affiliation: Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Tübingen, Federal Republic of Germany.
authors.forename: J A
authors.initials: JA
authors.lastname: Knoblich
authors.forename: K
authors.initials: K
authors.lastname: Sauer
authors.forename: L
authors.initials: L
authors.lastname: Jones
authors.forename: H
authors.initials: H
authors.lastname: Richardson
authors.forename: R
authors.initials: R
authors.lastname: Saint
authors.forename: C F
authors.initials: CF
authors.lastname: Lehner
mesh_terms: D000818:Animals; D001483:Base Sequence; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002453:Cell Cycle; D016213:Cyclins / Q000502:physiology*; D017931:DNA Primers / Q000737:chemistry; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology*; D005786:Gene Expression Regulation; D006131:Growth Inhibitors; D008938:Mitosis; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016196:S Phase
chemical_list: D016213:Cyclins; D017931:DNA Primers; D006131:Growth Inhibitors; D012333:RNA, Messenger; D016203:CDC2 Protein Kinase