Gene expression and transcript size of the prepro-peptide VIP/PHM-27 in normal human tissue.
The transcript size of VIP/PHM-27 mRNA (vasoactive intestinal peptide/peptide histidine methionine) and the relative distribution of VIP/PHM-27 gene expression in 10 normal human tissues was examined. After mRNA extraction from tissue, VIP/PHM-27 transcript size and relative abundance of mRNA was determined by Northern blot analysis and densitometry of the autoradiograms. VIP/PHM-27 mRNA was detectable in brain, pancreas, colon, ileum and striated muscle while no hybridization signal was observed in liver, kidney, lung, heart, prostate and placental tissue. VIP/PHM-27 transcript in human brain and gut was a single band of 1.7 kb; by contrast, a 7.0-kb transcript was detected in striated skeletal muscle. The highest relative levels of mRNA were observed in brain and pancreas.
Schulte-Bockholt
A
A
Division of Gastroenterology, Zablocki VA Medical Center, Milwaukee, WI.
Meier
D A
DA
Hopp
K
K
Stadelmann
A
A
Otterson
M F
MF
Telford
G L
GL
Koch
T R
TR
D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D005091:Exons / Q000502:physiology; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D017403:In Situ Hybridization; D010451:Peptide PHI / Q000096:biosynthesis* / Q000235:genetics; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000096:biosynthesis* / Q000235:genetics
D010451:Peptide PHI; D011498:Protein Precursors; D012333:RNA, Messenger; D014660:Vasoactive Intestinal Peptide