A homologue of the 65 kDa regulatory subunit of protein phosphatase 2A in early pea (Pisum sativum L.) embryos.
A partial cDNA, isolated from an early developing pea (Pisum sativum L.) embryo library, was found to encode a plant homologue of the regulatory subunit (PR65) of protein phosphatase 2A (PP2A). Comparison of the deduced amino acid sequence with a human PR65 sequence showed that the regulatory subunit of PP2A has been highly conserved during evolution. Southern analysis demonstrated that in pea and rape the catalytic and regulatory subunits of PP2A are encoded by multigene families. The levels of the transcripts encoding each subunit are developmentally regulated during pea embryogenesis and expression of the regulatory subunit is not solely restricted to the embryo.
Evans
I M
IM
Department of Biological Sciences, University of Durham, UK.
Fawcett
T
T
Boulter
D
D
Fordham-Skelton
A P
AP
D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D007887:Fabaceae / Q000196:embryology / Q000201:enzymology*; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010946:Plants, Medicinal; D054648:Protein Phosphatase 2; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D018076:DNA, Complementary; C432785:PPP2R1B protein, human; D010940:Plant Proteins; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2