title: Simultaneous determination of felbamate and three metabolites in human plasma by high-performance liquid chromatography.
abstract: An isocratic liquid-chromatographic method employing one extraction step and a 4.6 mm x 150 mm Spherisorb ODS2, 3 microns high-performance liquid chromatography (HPLC) column using ultraviolet (UV)-absorbance detection at 210 nm has been developed for quantitation of felbamate (FBM) and three felbamate metabolites in 0.100-ml aliquots of human plasma. The linear quantitation range for FBM and the two hydroxy metabolites is 0.781-200 micrograms/ml, and that for the monocarbamate metabolite is 0.391-200 micrograms/ml.
authors.affiliation: Department of Biochemistry, Wallace Laboratories, Cranbury, New Jersey 08512.
authors.forename: L A
authors.initials: LA
authors.lastname: Romanyshyn
authors.forename: J K
authors.initials: JK
authors.lastname: Wichmann
authors.forename: N
authors.initials: N
authors.lastname: Kucharczyk
authors.forename: R D
authors.initials: RD
authors.lastname: Sofia
mesh_terms: D000927:Anticonvulsants / Q000097:blood* / Q000493:pharmacokinetics; D001322:Autoanalysis; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D000078328:Felbamate; D006801:Humans; D048448:Phenylcarbamates; D011409:Propylene Glycols / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000927:Anticonvulsants; D048448:Phenylcarbamates; D011409:Propylene Glycols; D000078328:Felbamate