Effect of thimerosal on cytosolic calcium and phosphatidylserine synthesis in Jurkat T cells.
1. We investigated the effect of the thiol reagent, thimerosal on calcium movements in the Jurkat T cell line. 2. Thimerosal induced a rise in cytosolic Ca2+ concentration due both to a release of Ca2+ from intracellular stores and a Ca2+ influx. 3. Thimerosal, released Ca2+ from the same intracellular stores than CD3 mAb and ionomycin. 4. Emptying the Ca2+ intracellular stores was accompanied by a marked decrease of phosphatidylserine synthesis indicating that phosphatidylserine synthesis occurs within or close to the endoplasmic reticulum Ca(2+)-stores as previously described in CD3-, ionomycin- or Ca(2+)-ATPase inhibitor-treated lymphocytes.
Pelassy
C
C
INSERM U343, Faculté de Médecine, Nice, France.
Breittmayer
J P
JP
Ticchioni
M
M
Aussel
C
C
D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003600:Cytosol / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D010718:Phosphatidylserines / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013849:Thimerosal / Q000494:pharmacology*
D010718:Phosphatidylserines; D013849:Thimerosal; D002118:Calcium