Alkaline phosphatase in human semen: an investigation using enzyme inhibitors and gel electrophoresis.
Human seminal alkaline phosphatase was investigated with respect to its electrophoretic mobility, heat lability, and susceptibility to inhibition by phenylalanine, tartrate, and homoarginine. Total alkaline phosphatase activity in 30 samples of human semen was measured colorimetrically, using p-nitrophenylphosphate as substrate. Using linear regression analysis, no significant correlation was found between the enzyme activity and the sperm count, sperm motility, semen volume, and the concentrations of seminal inositol and fructose. The alkaline phosphatase activity was higher in the earlier portion of split ejaculate samples. Sodium DL-tartrate (42 mmol/l), which inhibits acid phosphatase, did not inhibit seminal alkaline phosphatase significantly. L-Homoarginine (10 mmol/l), an inhibitor of the liver and bone isoenzymes, inhibited the seminal enzyme (53%), whereas L-phenylalanine (12 mmol/l), a strong inhibitor of placental alkaline phosphate, decreased activity by about 10%. Electrophoresis of semen samples on agarose revealed a broad band which was not sharpened after treatment with neuraminidase. Semen total alkaline phosphatase was essentially totally inactivated by heating at 56 degrees C for 15 min or 10 min at 65 degrees C; similar behaviour has been reported for the liver and bone isoenzymes. Electrophoresis after heating did not reveal a residual band of heat-stable placental-like alkaline phosphatase. Semen alkaline phosphatase appears to contain more than one isoenzyme, but placental-like alkaline phosphatase cannot be more than a minor component.
Lewin
L M
LM
Dept. of Chemical Pathology, Sackler Medical School, Tel Aviv University, Israel.
Golan
R
R
Soffer
Y
Y
Kaufman
S
S
Yulzary
Y
Y
Zaidman
J
J
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D002845:Chromatography; D003124:Colorimetry; D004587:Electrophoresis, Agar Gel; D005632:Fructose / Q000378:metabolism; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007527:Isoenzymes / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D009596:Nitrophenols / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D012044:Regression Analysis; D012661:Semen / Q000737:chemistry / Q000201:enzymology*; D013076:Sperm Count; D013081:Sperm Motility
D007295:Inositol Phosphates; D007527:Isoenzymes; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D005632:Fructose; C008644:nitrophenylphosphate; D000469:Alkaline Phosphatase