title: Involvement of SRE element of Ty1 transposon in TEC1-dependent transcriptional activation.
abstract: Some Ty1 transposable element insertion mutations of Saccharomyces cerevisiae activate transcription of adjacent genes in a cell-type dependent manner. This activation requires at least STE12 and TEC1 gene products. The binding site for the STE12 protein is located in the sterile responsive element (SRE), which is just downstream the 5' LTR of Ty1 and contains one copy of the pheromone response element (PRE). This report defines the sequences in Ty1 required for TEC1-dependent activation using a TDH3::lacZ reporter gene in which the UAS was replaced by different portions of a Ty1 or Ty2 element. The Ty1 SRE seems to be sufficient to ensure the TEC1 and STE12-mediated activation whereas Ty2 SRE can activate the expression of the adjacent genes in the absence of both proteins. Adjacent to the PRE element, there is a region (PAE) with extensive sequence divergence in Ty1 and Ty2 SREs. Swapping experiments between Ty1 and Ty2 sequences show that Ty1 PAE is required for the activation of adjacent gene expression in a TEC1 and STE12-dependent manner. The use of a LexA::TEC1 construct indicates that the chimeric protein has no activation ability suggesting that TEC1 could act in conjunction with another factor.
authors.affiliation: Laboratoire de Microbiologie, Université Libre de Bruxelles, Belgium.
authors.forename: I
authors.initials: I
authors.lastname: Laloux
authors.forename: E
authors.initials: E
authors.lastname: Jacobs
authors.forename: E
authors.initials: E
authors.lastname: Dubois
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D004742:Enhancer Elements, Genetic; D005656:Fungal Proteins / Q000494:pharmacology; D018014:Gene Transfer Techniques; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017385:Sequence Homology; D013539:Simian virus 40 / Q000235:genetics; D014157:Transcription Factors / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D004251:DNA Transposable Elements; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; C489241:STE12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C069926:TEC1 protein, S cerevisiae; D014157:Transcription Factors