Toxic effects of fatty acid anilides on the oxygen defense systems of guinea pig lungs and erythrocytes.
Toxic oil syndrome (TOS) is caused by ingestion of denatured edible oils. Even though the etiology and pathogenesis of this disease are not fully known, it is quite clear that generation of free radicals caused by ingestion of fatty acid anilides is responsible for the pathogenetic mechanism in many TOS patients. Fatty acid anilides may also alter the free radical status of lungs and erythrocytes; this possibility may shed some light on understanding toxic oil syndrome. The present study describes the effects of oral administration of fatty acid anilides on the activities of major enzymes involved in the oxygen defense systems of lungs and erythrocytes. Feeding fatty acid anilides caused an increase in the superoxide dismutase (SOD) activity in erythrocytes, whereas it caused a decrease in the SOD activity in lungs. GSH-Px activity was not significantly changed in erythrocytes but was decreased in lungs. Although the activity of catalase was increased only by a higher dose in the erythrocytes, it was not affected in the lung at any dosage. Even though the ingestion of fatty acid anilides caused an increase in the SOD activity in the erythrocytes and a decrease in the SOD activity in the lungs, there was an increase in the lipid peroxidation in both cases. The increase in lipid peroxidation in erythrocytes is probably caused by the accumulation of H2O2, and that in the lungs is due to the accumulation of superoxide anion.
Mukherjee
S
S
Department of Biochemistry, Meharry Medical College, Nashville, TN 37208.
Ghosh
S
S
Rodgers
L
L
Nayyar
T
T
Desai
U
U
Das
S K
SK
D000813:Anilides / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001937:Brassica; D002374:Catalase / Q000097:blood / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005229:Fatty Acids, Monounsaturated; D005609:Free Radicals; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D006168:Guinea Pigs; D008041:Linoleic Acids / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008168:Lung / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009829:Oleic Acids / Q000633:toxicity*; D010938:Plant Oils / Q000506:poisoning; D000074262:Rapeseed Oil; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
D000813:Anilides; D005229:Fatty Acids, Monounsaturated; D005609:Free Radicals; D008041:Linoleic Acids; D009829:Oleic Acids; D010938:Plant Oils; D000074262:Rapeseed Oil; C039294:linoleylanilide; C038731:oleoylanilide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase