title: FTZ-F1-related orphan receptors in Xenopus laevis: transcriptional regulators differentially expressed during early embryogenesis.
abstract: Orphan receptors of the FTZ-F1-related group of nuclear receptors (xFF1r) were identified in Xenopus laevis by isolation of cDNAs from a neurula stage library. Two cDNAs were found, which encode full length, highly related receptor proteins, xFF1rA and B, whose closet relative known so far is the murine LRH-1 orphan receptor. xFF1rA protein expressed by a recombinant vaccinia virus system specifically binds to FTZ-F1 response elements (FRE; PyCAAGGPyCPu). In cotransfection studies, xFF1rA constitutively activates transcription, in a manner dependent on the number of FREs. The amounts of at least four mRNAs encoding full-length receptors greatly increase between gastrula and early tailbud stages and decrease at later stages. At early tailbud stages, xFTZ-F1-related antigens are found in all nuclei of the embryo.
authors.affiliation: Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Ellinger-Ziegelbauer
authors.forename: A K
authors.initials: AK
authors.lastname: Hihi
authors.forename: V
authors.initials: V
authors.lastname: Laudet
authors.forename: H
authors.initials: H
authors.lastname: Keller
authors.forename: W
authors.initials: W
authors.lastname: Wahli
authors.forename: C
authors.initials: C
authors.lastname: Dreyer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000502:physiology*; D051717:Fushi Tarazu Transcription Factors; D015870:Gene Expression; D005914:Globins / Q000235:genetics; D006367:HeLa Cells; D018398:Homeodomain Proteins; D006801:Humans; D019476:Insect Proteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid / Q000096:biosynthesis*; D015183:Restriction Mapping; D054339:Steroidogenic Factor 1; D014157:Transcription Factors / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014162:Transfection; D014982:Xenopus laevis / Q000196:embryology
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D054339:Steroidogenic Factor 1; D014157:Transcription Factors; C497169:ftz protein, Drosophila; C499712:nuclear hormone receptor FTZ-F1, Drosophila; D005914:Globins