title: Detection of Salmonella enteritidis in feces from poultry using booster polymerase chain reaction and oligonucleotide primers specific for all members of the genus Salmonella.
abstract: Salmonella enteritidis was identified in feces from hens using the polymerase chain reaction (PCR) and oligonucleotide primers specific for all members of the genus Salmonella. Feces from specific-pathogen-free Leghorn hens were determined to be negative for Salmonella by microbiological culture and by the PCR. Fecal samples were inoculated with known numbers of colony-forming units of S. enteritidis. The DNA was extracted from fecal samples and amplified by the PCR using genus-specific primers. Salmonella were detected in all samples known to be positive; the sensitivity of the assay extended to 1 cfu of S. enteritidis/g feces. Feces that were not inoculated with Salmonella were negative. Microbiological culture was less sensitive than the PCR assay; results of culture of feces with less than 10(2) cfu/g were negative. Although S. enteritidis was used in this study, the oligonucleotide primers used in this study have been previously demonstrated to be genus-specific for Salmonella.
authors.affiliation: Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843.
authors.forename: N D
authors.initials: ND
authors.lastname: Cohen
authors.forename: E D
authors.initials: ED
authors.lastname: McGruder
authors.forename: H L
authors.initials: HL
authors.lastname: Neibergs
authors.forename: R W
authors.initials: RW
authors.lastname: Behle
authors.forename: D E
authors.initials: DE
authors.lastname: Wallis
authors.forename: B M
authors.initials: BM
authors.lastname: Hargis
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000382:microbiology*; D017931:DNA Primers; D005243:Feces / Q000382:microbiology*; D005260:Female; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012477:Salmonella enteritidis / Q000302:isolation & purification*; D013045:Species Specificity
chemical_list: D017931:DNA Primers