Diagnosis of major chromosome aneuploidies in human preimplantation embryos.
A short fluorescence in-situ hybridization (FISH) procedure using fluorochrome and digoxigenin labelled DNA probes was developed for application in human preimplantation embryos in order to analyse the five chromosomes most involved in human aneuploidy (X, Y, 18, 13 and 21). The chromosomes were fluorescent-stained and detected simultaneously in 157 blastomeres from 30 human embryos. Successful FISH analysis was achieved in 93% of the blastomeres. Aberrations for these chromosomes were found in 70% of abnormally developing monospermic embryos. The majority of normally developing monospermic embryos obtained from older patients were also chromosomally abnormal. By analysing all or most of the cells from these embryos, true mosaicism was distinguished from technique failure. Mosaic embryos, polyploid embryos with ploidies as high as 8n, haploid embryos, embryos monosomic for 13/21 and for X, and embryos trisomic for 13/21 and 18, were common in abnormally developing embryos. In contrast, aneuploidy was the main chromosome abnormality found in normally developing monospermic embryos.
Munné
S
S
Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, NY 10021.
Lee
A
A
Rosenwaks
Z
Z
Grifo
J
J
Cohen
J
J
D000328:Adult; D000782:Aneuploidy; D001755:Blastocyst / Q000502:physiology*; D001757:Blastomeres; D002869:Chromosome Aberrations / Q000175:diagnosis; D025063:Chromosome Disorders; D002882:Chromosomes, Human, Pair 13; D002887:Chromosomes, Human, Pair 18; D002891:Chromosomes, Human, Pair 21; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes; D008423:Maternal Age; D018566:Pregnancy, High-Risk; D014960:X Chromosome; D014998:Y Chromosome