Rat heart fatty acid-binding protein content is increased in experimental diabetes.
Cytoplasmic fatty acid-binding protein (FABP) was assayed immunochemically in hearts from rats with insulin-dependent (IDDM) or non-insulin-dependent diabetes mellitus (NIDDM). FABP contents were 34% higher in IDDM and 103% higher in NIDDM hearts than in respective age-matched controls. FABP levels returned to control values when islets of Langerhans were transplanted into diabetic IDDM animals. In the diabetic hearts the activity of fructose-6-phosphate kinase decreased (IDDM and NIDDM animals), while that of 3-hydroxyacyl-CoA dehydrogenase increased (NIDDM animals only). These data indicate that experimental diabetes induces a marked increase of the FABP content of rat heart and suggests that this protein is involved in the enhanced fatty acid utilization by the diabetic heart.
Glatz
J F
JF
Dept. of Physiology, Cardiovascular Research Institute Maastricht, The Netherlands.
van Breda
E
E
Keizer
H A
HA
de Jong
Y F
YF
Lakey
J R
JR
Rajotte
R V
RV
Thompson
A
A
van der Vusse
G J
GJ
Lopaschuk
G D
GD
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000378:metabolism*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005951:Glucose Tolerance Test; D006029:Glycosuria; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D011914:Rats, Inbred BN; D011920:Rats, Inbred WF; D012016:Reference Values
D001786:Blood Glucose; D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D007328:Insulin; D008055:Lipids; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins