Cloning and characterization of a chromosomal gene cluster, pah, that encodes the upper pathway for phenanthrene and naphthalene utilization by Pseudomonas putida OUS82.
A 25-kb DNA SalI fragment cloned from the chromosomal DNA of Pseudomonas putida OUS82, which utilizes phenanthrene (Phn+) and naphthalene (Nah+), carried all of the genes necessary for upper naphthalene catabolism. Cosmid recombinant pIP7 complemented both the Nah- and Phn- defects of OUS8211 (Trp-Nah-Phn-Sal+[salicylate utilizing]Hna+[1-hydroxy-2-naphthoate utilizing]) and only the Phn- defect of OUS8212 (Trp-Nah-Phn-Sal-Hna+). The results indicate that strain OUS82 uses different pathways after o-hydroxycarboxylic aromatics in the catabolism of naphthalene and phenanthrene.
Kiyohara
H
H
Department of Applied Chemistry, Faculty of Engineering, Okayama University of Science, Japan.
Torigoe
S
S
Kaida
N
N
Asaki
T
T
Iida
T
T
Hayashi
H
H
Takizawa
N
N
D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics* / Q000502:physiology; D005810:Multigene Family / Q000235:genetics* / Q000502:physiology; D009281:Naphthalenes / Q000378:metabolism*; D010616:Phenanthrenes / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D013379:Substrate Specificity / Q000235:genetics
D009281:Naphthalenes; D010616:Phenanthrenes; C031721:naphthalene; C031181:phenanthrene