[Study of segments of 23S RNA, important for interaction with elongation factors Tu and G using complementary DNA-oligonucleotides].
Several oligonucleotides complementary to different 23S RNA regions were tested in the elongation factor-dependent reactions of the ribosomes. It was found that the 1088-1100 and 1127-1140 sequence parts of the 23S RNA (binding regions for the L11 protein) are very important for EF-G function. The EF-Tu function is markedly less affected by these nucleotides. The probable role of 23S RNA function is discussed.
Bubunenko
M G
MG
Gudkov
A T
AT
D018076:DNA, Complementary; D009841:Oligonucleotides; D020653:Peptide Elongation Factor G; D010444:Peptide Elongation Factor Tu / Q000737:chemistry / Q000378:metabolism*; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000378:metabolism*; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000378:metabolism*
D018076:DNA, Complementary; D009841:Oligonucleotides; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D012338:RNA, Ribosomal, 23S; D010444:Peptide Elongation Factor Tu
Issledovanie uchastkov 23S RNK, vazhnykh dlia vzaimodeĭstviia s faktorami élongatsii Tu i G, s pomoshch'iu komplementarnykh DNA-oligonukleotidov.