title: Yeast mitochondrial calcium uptake: regulation by polyamines and magnesium ions.
abstract: Spermine, spermidine, and magnesium ions modulate the kinetic parameters of the Ca2+ transport system of Endomyces magnusii mitochondria. Mg2+ at concentrations up to 5 mM partially inhibits Ca2+ transport with a half-maximal inhibiting concentration of approximately 0.5 mM. In the presence of 2 mM MgCl2, the S0.5 value of the Ca2+ transport system increases from 220 to 490 microM, which indicates decreased affinity for the system. Spermine and spermidine exert an activating effect, having half-maximal concentrations of 12 and 50 microM, respectively. In the case of spermine, the S0.5 value falls to 50-65 microM, which implies an increase in the transport system affinity for Ca2+. Both Mg2+ and spermine cause a decrease of the Hill coefficient, giving evidence for a smaller degree of cooperativity. Spermine and spermidine enable yeast mitochondria to remove Ca2+ from the media completely. In contrast, Mg2+ lowers the mitochondrial buffer capacity. When both Mg2+ and spermine are present in the medium, their effects on the S0.5 value and the free extramitochondrial Ca2+ concentration are additive. The ability of spermine and Mg2+ to regulate yeast mitochondrial Ca2+ transport is discussed.
authors.affiliation: A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow.
authors.forename: T V
authors.initials: TV
authors.lastname: Votyakova
authors.forename: E N
authors.initials: EN
authors.lastname: Bazhenova
authors.forename: R A
authors.initials: RA
authors.lastname: Zvjagilskaya
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism* / Q000494:pharmacology; D004718:Saccharomycetales / Q000187:drug effects / Q000378:metabolism*
chemical_list: D011073:Polyamines; D008274:Magnesium; D002118:Calcium