title: Adherence pattern of non-pilated Aeromonas hydrophila strains to tissue cultures.
abstract: Adherence of non-pilated Aeromonas hydrophila strains to HEp-2, HeLa, CHO and Vero tissue culture cells was studied. The strains were isolated from intestinal and extra-intestinal human infections and from the environment. The environmental isolates revealed a diffuse type of adherence to all types of cell lines while clinical isolates showed localized adherence (50%). Strains revealing the diffuse type of adherence showed high levels of adhesion i.e. > 20 bacteria per cell. This activity correlated with hydrophobicity of the strains tested. Haemagglutination assay with human erythrocytes was negative, showing no association with plasmid profile, Congo red binding and adherence to tissue culture cells. Thus the experiments showed that in non-pilated strains, hydrophobicity may be the major factor responsible for adherence to epithelial cells.
authors.affiliation: Institute of Preventive and Clinical Medicine, Bratislava, Slovak, Republic.
authors.forename: G
authors.initials: G
authors.lastname: Bartková
authors.forename: I
authors.initials: I
authors.lastname: Ciznár
mesh_terms: D016980:Aeromonas hydrophila / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D005759:Gastroenteritis / Q000382:microbiology; D006386:Hemagglutination Tests; D006801:Humans; D010957:Plasmids / Q000032:analysis; D012141:Respiratory Tract Infections / Q000382:microbiology; D014552:Urinary Tract Infections / Q000382:microbiology; D014774:Virulence; D014871:Water Microbiology