Preparation and physical properties of conjugates of oligodeoxynucleotides with poly(delta)ornithine peptides.
Oligodeoxynucleotides (12-mers) having either a 3' aminolinker or both 3' and 5' aminolinker groups were synthesized and then reacted with N-iodoacetoxysuccinimide. Separately, a series of peptides, consisting of cysteine carboxyamide and a varying number of residues of ornithine coupled through their side-chain amino groups, was prepared, leaving on the final Fmoc protecting group. Reaction of each Fmoc-peptide with an activated oligodeoxynucleotide yielded the desired conjugates, which were purified by an Fmoc-on/Fmoc-off two-step reversed-phase HPLC procedure. On hybridization with a complementary unmodified 12-mer oligodeoxynucleotide, it was found that there is a gradual increase in melting temperature with the number of ornithine residues, whereas the appended peptide did not perturb the B form of the duplex.
Zhu
T
T
Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854.
Tung
C H
CH
Breslauer
K J
KJ
Dickerhof
W A
WA
Stein
S
S
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D010455:Peptides; C008973:polyornithine