Sodium selenite as modulator of red cell shape.
Addition of sodium selenite to human red cells, under ATP deplete conditions, induces a rapid oxidation of both glutathione and protein sulphydryl groups. Selenite also inhibits the discocyte-echinocyte shape transformation and stops the process before completion. Parallel to the effect on shape, selenite reduces the dephosphorylation of phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-monophosphate. Therefore our results support a shape change mechanism based on the metabolism of phosphoinositides and compatible with the bilayer-couple hypothesis.
Björk
J
J
Department of Biochemistry, University of Umeå, Sweden.
Backman
L
L
D000255:Adenosine Triphosphate / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D018038:Sodium Selenite / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000378:metabolism
D001798:Blood Proteins; D018129:Phosphatidylinositol Phosphates; D013438:Sulfhydryl Compounds; C037178:phosphatidylinositol 4-phosphate; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C068961:phosphatidylinositol 4,5-biphosphate kinase; D005978:Glutathione; D018038:Sodium Selenite