Identification of domains in bluetongue virus VP3 molecules essential for the assembly of virus cores.
Bluetongue virus (BTV) cores consist of the viral genome and five proteins, including two major components (VP3 and VP7) and three minor components (VP1, VP4, and VP6). VP3 proteins form an inner scaffold for the deposition on the core of the surface layer of VP7. VP3 also encapsidates and interacts with the three minor proteins. The BTV VP3 protein consists of 901 amino acids and has a sequence that is a highly conserved among BTV serotypes and other orbiviruses (e.g., epizootic hemorrhagic disease virus and African horse sickness virus). To locate sites of interaction between VP3 and the other structural proteins, we have analyzed the effects of a number of VP3 deletion mutants representing conserved regions of the protein, using as an assay the formation of core-like particles (CLPs) expressed by recombinant baculoviruses. Five of the VP3 deletion mutants interacted with the coexpressed VP7 and made CLPs. These CLPs also incorporated the three minor proteins. One mutant, lacking VP3 amino acid residues 499 to 508, failed to make CLPs. Further mutational analyses have demonstrated that a methionine at residue 500 of VP3 and an arginine at residue 502 were both required for CLP formation.
Tanaka
S
S
Laboratory of Molecular Biophysics, University of Oxford, United Kingdom.
Roy
P
P
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D001820:Bluetongue virus / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins; D017124:Conserved Sequence / Q000235:genetics; D004252:DNA Mutational Analysis; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009036:Moths / Q000166:cytology; D016297:Mutagenesis, Site-Directed; D017924:Nucleopolyhedroviruses / Q000235:genetics; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D014758:Viral Core Proteins / Q000378:metabolism*
D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus; D014758:Viral Core Proteins