An attempt to identify gene products related to the induction of an antiviral state in macrophages resistant and sensitive to IFN-gamma.
The activation of bone-marrow-derived macrophages by IFN-gamma (IFN gamma) partially inhibits mouse hepatitis virus 3 (MHV3) replication only in cells from resistant A/J mice, and not in cells originating from susceptible BALB/c mice. The computer image analysis of gels obtained from 2D-SDS-PAGE of extracted proteins of IFN gamma-activated A/J or BALB/c macrophages enabled us to identify and tag several gene products that were synthesized at elevated or diminished levels. Comparisons of the patterns of non-activated and IFN gamma-activated A/J macrophages revealed 3 gene products which increased, 1 which newly appeared, 6 which decreased and 20 which disappeared upon IFN gamma activation. The protein pattern of BALB/c macrophages revealed 13 gene products which increased, 8 which decreased and 8 which disappeared in IFN gamma-activated BALB/c macrophages. Whether these proteins are involved in the induction of an antiviral state against MHV3 growth remains to be investigated. Macrophages from mice with different genetic background (A/J and BALB/c), upon IFN gamma activation, behave differently at a molecular level, and this observation is consistent with their distinct expression of antiviral state against MHV3.
Pereira
C A
CA
Instituto Butantan, Laboratorio de Immunologia Viral, Sao Paulo, Brazil.
Lucchiari
M A
MA
Modolell
M
M
Kuhn
L
L
Lefkovits
I
I
D000818:Animals; D004351:Drug Resistance; D015180:Electrophoresis, Gel, Two-Dimensional; D007371:Interferon-gamma / Q000494:pharmacology* / Q000502:physiology; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D006517:Murine hepatitis virus / Q000276:immunology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000276:immunology; D014779:Virus Replication / Q000276:immunology
D011506:Proteins; D007371:Interferon-gamma