title: Calcium level affects the efficacy of supplemental microbial phytase in corn-soybean meal diets of weanling pigs.
abstract: A 2 x 2 x 2 factorial experiment was conducted with 64 pigs (4 wk old, 8.04 +/- .50 kg BW) to determine the effect of various dietary concentrations of Ca, vitamin D, and microbial phytase (Aspergillus niger) on phytate-P utilization. A low-P, corn-soybean meal diet was supplemented with two levels of phytase (unit/gram), 750 (suboptimal) and 1,200 (optimal); of vitamin D (international unit/kilogram), 660 (normal) and 6,660 (high); and of Ca (percentage), .4 (low) and .8 (normal). Pen feed consumption and individual pig weights, plasma inorganic P and Ca concentrations, and plasma alkaline phosphatase (AP) activity were measured at d 10, 20, and 30. The normal dietary Ca concentration had an adverse effect (P < .05) on all the response measures. The depressive effect of the normal dietary Ca on performance was greater (P < .05) at the normal vitamin D level or at the optimal phytase level than at the other levels of these two factors. The elevation in plasma AP activity in pigs fed the normal dietary Ca was greater (P < .05) at the suboptimal than at the optimal phytase level. The decreases in plasma inorganic P concentration and increases in plasma Ca concentration associated with the normal dietary Ca were substantial. In conclusion, the normal level of Ca in the diet greatly reduced the efficacy of supplemental phytase. Raising vitamin D in the diet partially offset this adverse effect but did not produce further improvement when the Ca level was low.
authors.affiliation: Animal Science Department, Michigan State University, East Lansing 48824.
authors.forename: X G
authors.initials: XG
authors.lastname: Lei
authors.forename: P K
authors.initials: PK
authors.lastname: Ku
authors.forename: E R
authors.initials: ER
authors.lastname: Miller
authors.forename: M T
authors.initials: MT
authors.lastname: Yokoyama
authors.forename: D E
authors.initials: DE
authors.lastname: Ullrey
mesh_terms: D010832:6-Phytase / Q000008:administration & dosage / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000097:blood; D000821:Animal Feed; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D001682:Biological Availability; D002118:Calcium / Q000097:blood; D002136:Calcium, Dietary / Q000008:administration & dosage*; D004435:Eating; D005527:Food, Fortified; D010758:Phosphorus / Q000097:blood; D016226:Phosphorus, Dietary / Q000493:pharmacokinetics; D010833:Phytic Acid / Q000493:pharmacokinetics; D011897:Random Allocation; D013025:Glycine max; D013552:Swine / Q000097:blood / Q000254:growth & development / Q000378:metabolism*; D014807:Vitamin D / Q000008:administration & dosage; D014886:Weaning; D015430:Weight Gain; D003313:Zea mays
chemical_list: D002136:Calcium, Dietary; D016226:Phosphorus, Dietary; D014807:Vitamin D; D010758:Phosphorus; D010833:Phytic Acid; D000469:Alkaline Phosphatase; D010832:6-Phytase; D002118:Calcium