Eicosapentaenoic acid and docosahexaenoic acid suppress the proliferation of vascular smooth muscle cells.
Eicosapentaenoic acid, which is one of the n-3 polyunsaturated fatty acids (PUFA), is reported to exert its antithrombotic and anti-atherogenic effect partly through the modulation of vascular cell functions. Vascular smooth muscle cell (VSMC) proliferation plays an important role in the pathogenesis of atherosclerosis. We reported the differential effect of various PUFA on VSMC proliferation. First we established a method for preparing PUFA rich cells in culture to mimic the in vivo situation using PUFA triacylglycerol emulsion. Using these fatty acid rich cells, we found that only EPA and docosahexaenoic acid, although less potent than EPA, inhibited the proliferation of VSMC among the fatty acids tested. This effect of EPA was reversed by the addition of anti-oxidants. It is suggested that production of the oxidized species at a low concentration from EPA inhibited the proliferation of VSMC. This anti-proliferative effect of EPA and DHA on VSMC could partly explain the anti-atherosclerotic effect of marine lipids.
Shiina
T
T
Second Department of Internal Medicine, Chiba University School of Medicine, Chuo-Ku, Japan.
Terano
T
T
Saito
J
J
Tamura
Y
Y
Yoshida
S
S
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000378:metabolism; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D007213:Indomethacin / Q000494:pharmacology; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D051381:Rats; D011921:Rats, Inbred WKY; D014810:Vitamin E / Q000494:pharmacology
D008041:Linoleic Acids; D008054:Lipid Peroxides; D009829:Oleic Acids; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D004281:Docosahexaenoic Acids; D019301:Oleic Acid; D004247:DNA; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid; D007213:Indomethacin