title: Connectin content in rabbit cardiac and skeletal muscle.
abstract: 1. The contents of connectin in rabbit cardiac and skeletal muscle were estimated by densitometry of the bands in SDS gel electrophoresis. 2. The weight ratio of connectin to myosin was 1:9.5 in cardiac muscle and 1:4.2 in skeletal muscle. 3. Assuming that the molecular weights of myosin and skeletal muscle connectin were 5.25 x 10(5) and 4.2 x 10(6) respectively, and a myosin filament consists of 300 myosin molecules, it was calculated that there were approx. 4 connectin molecules per half of myosin filament in rabbit skeletal muscle. 4. In cardiac muscle, the molecular weight of connectin was assumed to be 3.6 x 10(6) and it was estimated that there were about 2 connectin filaments per half of myosin filament.
authors.affiliation: Department of Biology, Faculty of Science, Chiba University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
mesh_terms: D000818:Animals; D064211:Connectin; D004591:Electrophoresis, Polyacrylamide Gel; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry*; D009218:Myosins / Q000032:analysis; D011494:Protein Kinases; D011817:Rabbits
chemical_list: D064211:Connectin; D009124:Muscle Proteins; D011494:Protein Kinases; D009218:Myosins