title: Association of poly(CA).poly(TG) DNA fragments into four-stranded complexes bound by HMG1 and 2.
abstract: The tandemly repeated DNA sequence poly(CA).poly(TG) is found in tracts up to 60 base pairs long, dispersed at thousands of sites throughout the genomes of eukaryotes. Double-stranded DNA fragments containing such sequences associated spontaneously with each other in vitro, in the absence of protein, forming stable four-stranded structures that were detected by gel electrophoresis and electron microscopy. These structures were recognized specifically by the nuclear nonhistone high mobility group (HMG) proteins 1 and 2 as evidenced by gel retardation. Such sequence-specific complexes might be involved in vivo in recombination or other processes requiring specific association of two double-stranded DNA molecules.
authors.affiliation: Institut Jacques Monod, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Gaillard
authors.forename: F
authors.initials: F
authors.lastname: Strauss
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000378:metabolism*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D006609:High Mobility Group Proteins; D011089:Polydeoxyribonucleotides; D004247:DNA