title: Inhibitor analysis of calf thymus DNA polymerases alpha, delta and epsilon.
abstract: Quantitative effects of inhibitors of the replicative DNA polymerases (pol) alpha, delta and epsilon from calf thymus are reported under similar assay conditions. Carbonyldiphosphonate was a competitive inhibitor of pols delta and epsilon, with 4- to 6-fold selectivity compared to pol alpha. Aphidicolin inhibited pols alpha and delta with 6- to 10-fold selectivity compared to pol epsilon. The 'butylphenyl' nucleotides, BuPdGTP and BuAdATP, inhibited pol alpha with at least 1000-fold selectivity compared to pols delta and epsilon. The use of these inhibitors under similar assay conditions permits the discrimination of the three enzymes.
authors.affiliation: Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655.
authors.forename: G E
authors.initials: GE
authors.lastname: Wright
authors.forename: U
authors.initials: U
authors.lastname: Hübscher
authors.forename: N N
authors.initials: NN
authors.lastname: Khan
authors.forename: F
authors.initials: F
authors.lastname: Focher
authors.forename: A
authors.initials: A
authors.lastname: Verri
mesh_terms: D000818:Animals; D002417:Cattle; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D004258:DNA Polymerase III; D019384:Nucleic Acid Synthesis Inhibitors; D012680:Sensitivity and Specificity; D013950:Thymus Gland / Q000201:enzymology*
chemical_list: D019384:Nucleic Acid Synthesis Inhibitors; D004257:DNA Polymerase II; D004258:DNA Polymerase III