A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae.
The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the beta-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and susceptible isolates of M. leprae.
Honoré
N
N
Unité de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France.
Perrani
E
E
Telenti
A
A
Grosset
J
J
Cole
S T
ST
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics*; D003622:Dapsone / Q000627:therapeutic use; D004352:Drug Resistance, Microbial / Q000235:genetics; D004359:Drug Therapy, Combination; D005798:Genes, Bacterial; D006801:Humans; D015440:Leprosy, Lepromatous / Q000188:drug therapy / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D009166:Mycobacterium leprae / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011515:Prothionamide / Q000627:therapeutic use; D012008:Recurrence; D012293:Rifampin / Q000494:pharmacology* / Q000627:therapeutic use
D017931:DNA Primers; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D011515:Prothionamide; D003622:Dapsone; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin