Gene transfer studies of T cell receptor-gamma delta recognition. Specificity for staphylococcal enterotoxin A is conveyed by V gamma 9 alone.
gamma delta T cells bearing the V gamma 9 gene segment have been shown to recognize staphylococcal enterotoxin A (SEA) and a range of other Ags including mycobacterial Ags. We have established an experimental system to analyze the recognition properties of human TCR-gamma delta on a molecular level by transferring the receptor from its original T cell into a Jurkat T cell host that does not express an endogenous TCR. Three groups of transfectants that express the same delta-chain, V delta 1, but different gamma-chains (V gamma 9-J2-C gamma 2, V gamma 3-J2-C gamma 2, and V gamma 9-JP-C gamma 1) together with the endogenous CD3 were obtained. The transfectant T cells each expressing different gamma delta receptors all produced IL-2 after stimulation with plastic bound anti-CD3 Ab, but only those expressing V gamma 9 responded to stimulation with SEA in the presence of an autologous lymphoblastoid B cell line. In addition, transfectants that expressed V delta 2 combined with V gamma 9 could also respond to SEA. These results indicate that the V gamma 9 portion of the receptor, independent of the J region and C region or the delta-chain, is responsible for recognizing SEA.
Loh
E Y
EY
Department of Medicine, University of Pennsylvania, Philadelphia 19104.
Wang
M
M
Bartkowiak
J
J
Wiaderkiewicz
R
R
Hyjek
E
E
Wang
Z
Z
Kozbor
D
D
D000595:Amino Acid Sequence; D001483:Base Sequence; D017252:CD3 Complex / Q000502:physiology; D002460:Cell Line; D017931:DNA Primers / Q000737:chemistry; D004768:Enterotoxins / Q000276:immunology*; D018014:Gene Transfer Techniques; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology*; D013329:Structure-Activity Relationship
D017252:CD3 Complex; D017931:DNA Primers; D004768:Enterotoxins; D007376:Interleukin-2; D016692:Receptors, Antigen, T-Cell, gamma-delta; C026066:enterotoxin A, Staphylococcal