title: Stratum corneum lipid morphology and transepidermal water loss in normal skin and surfactant-induced scaly skin.
abstract: Stratum corneum lipid morphology was evaluated using attenuated total reflectance infrared spectroscopy (ATR-IR) in normal skin and surfactant-induced scaly skin to evaluate skin barrier function. To evaluate the degree of order of the intercellular lipid alkyl chain conformation, we measured the wavenumbers (frequency shifts) of the symmetrical and asymmetrical C-H stretching vibrations observed at approximately 2850 cm-1 and 2920 cm-1, respectively. There was a correlation between the wave-number and transepidermal water loss in normal skin. However, no difference was observed in surfactant-induced scaly skin from the baseline value in the wavenumbers of the C-H vibrations. These results suggest that in normal skin, lipid morphology plays an important role in the barrier function of the stratum corneum. However, the decline in barrier function in scaly skin is not due to conformational disorder of the lipid alkyl chain.
authors.affiliation: Shiseido Research Center, Yokohama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Denda
authors.forename: J
authors.initials: J
authors.lastname: Koyama
authors.forename: R
authors.initials: R
authors.lastname: Namba
authors.forename: I
authors.initials: I
authors.lastname: Horii
mesh_terms: D000328:Adult; D001834:Body Water / Q000378:metabolism; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D005712:Galvanic Skin Response / Q000187:drug effects / Q000502:physiology; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008297:Male; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D008055:Lipids; D012967:Sodium Dodecyl Sulfate