The Onchocerca volvulus mRNAs for a hsp70, a collagen-like protein and a ribosomal protein possess a 5' spliced leader sequence.
To detect and analyse Onchocerca volvulus mRNAs possessing a spliced leader (SL) sequence, a SL cDNA library was constructed from O. volvulus RNA using a PCR method. Three of the sixty cDNAs analysed displayed significant homology to a known sequence. These cDNAs encode a hsp70, a collagen-like protein and a 60S ribosomal protein. The remaining cDNAs analysed either encoded proteins with no apparent homology to known sequences or they appeared to be generated from RNAs with an internal SL and did not possess an open reading frame.
Henkle-Dührsen
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K
Bernhard Nocht Institute for Tropical Medicine, Department of Biochemistry, Hamburg, Germany.
Liebau
E
E
Walter
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RD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003094:Collagen / Q000737:chemistry / Q000235:genetics; D004247:DNA / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017181:Onchocerca volvulus / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D006360:Heat-Shock Proteins; D015801:Helminth Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D003094:Collagen; D004247:DNA