Induction of Alzheimer-like beta-amyloid immunoreactivity in the brains of rabbits with dietary cholesterol.
beta-amyloid and ALZ-50 immunocytochemical reactivity were determined in the brains of rabbits fed either a control or 2% cholesterol diet. Control rabbits demonstrated no accumulation of intracellular immunolabeled beta-amyloid within 3 min after death. In animals fed the experimental diet for 4, 6, and 8 weeks (postmortem interval < 3 min), there was an increasingly mild-to-moderate-to-severe accumulation of intracellular immunolabeled beta-amyloid. Whether or not beta-amyloid is causally linked to processes leading to dementia, it is related in some way to the prime cause of human death; heart disease. Hypercholesterolemic rabbits may provide an animal model to study altered beta-APP metabolism leading to Alzheimer-like beta-amyloid accumulation xe03and extracellular deposition in brain.
Sparks
D L
DL
Sanders-Brown Center on Aging, University of Kentucky Medical Center, Lexington 40536-0230.
Scheff
S W
SW
Hunsaker
J C
JC
Liu
H
H
Landers
T
T
Gross
D R
DR
D016229:Amyloid beta-Peptides / Q000032:analysis / Q000096:biosynthesis*; D000818:Animals; D000941:Antigens / Q000032:analysis / Q000096:biosynthesis*; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D005625:Frontal Lobe / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006937:Hypercholesterolemia / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis; D011817:Rabbits; D012016:Reference Values
C056167:Alzheimer's disease antigen; D016229:Amyloid beta-Peptides; D000941:Antigens; D002791:Cholesterol, Dietary; D009419:Nerve Tissue Proteins