title: Rapid determination of sparteine and its metabolites in urine.
abstract: A method is presented for the isolation, separation and determination of sparteine and its metabolites in urine. The isolation is based on rapid extraction with dichloromethane and pentane in a glass separator. For the separation and determination, capillary gas chromatography with nitrogen-phosphorus detection was used. The recovery of the method ranged from 81.6% to 94.8%, and the limit of determination varied between 0.2 and 0.5 microgram ml-1. For quantification, 17-ethylsparteine was used as the internal standard.
authors.affiliation: Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Veningerová
authors.forename: V
authors.initials: V
authors.lastname: Prachar
authors.forename: M
authors.initials: M
authors.lastname: Lukácsová
authors.forename: J
authors.initials: J
authors.lastname: Glasa
authors.forename: J
authors.initials: J
authors.lastname: Holomán
mesh_terms: D000328:Adult; D002849:Chromatography, Gas; D006801:Humans; D008875:Middle Aged; D010084:Oxidation-Reduction; D010641:Phenotype; D018154:Slovakia; D013034:Sparteine / Q000031:analogs & derivatives / Q000652:urine*
chemical_list: C044693:5-dehydrosparteine; D013034:Sparteine; C044694:2-dehydrosparteine