title: Synovial tissue implants from patients with rheumatoid arthritis cause cartilage destruction in knee joints of SCID.bg mice.
abstract: OBJECTIVE
  To establish in SCID.bg mice a model in which joint destruction is initiated by human inflammatory cells from patients with rheumatoid arthritis (RA).
  
  
  METHODS
  Development of a surgical technique and immunohistologic analysis.
  
  
  RESULTS
  Initial experiments with single cell suspensions failed because more than 70% of the cells injected intraarticularly left the mouse knee joint within 16 h without causing destruction. This was observed with peripheral blood mononuclear cells, T cell lines reactive to mouse or rat collagen type II, and synovial mononuclear cells. Cell immigration was reduced but not prevented by preactivation with mitogens. In contrast, small tissue implants from human synovial membrane which were transferred by surgical intervention into mouse knee joints remained at the site of injection and could be easily localized within the mouse joint (observation period up to 8 weeks). The human synovial membrane implants induced pannus formation and erosion of cartilage and bone while only a mild and transient synovitis was observed with normal synovial membrane and control tissues like human thymus. The predominant cells at the site of destruction were human (CD68+) and murine (Mac-2+) monocytes/macrophages.
  
  
  CONCLUSIONS
  The human/murine SCID arthritis is a useful model for studying pathogenetic aspects of joint destruction as well as effects of new drugs or novel treatment strategies.
authors.affiliation: Institute of Clinical Immunology, University of Leipzig, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Sack
authors.forename: H
authors.initials: H
authors.lastname: Kuhn
authors.forename: J
authors.initials: J
authors.lastname: Ermann
authors.forename: R W
authors.initials: RW
authors.lastname: Kinne
authors.forename: S
authors.initials: S
authors.lastname: Vogt
authors.forename: D
authors.initials: D
authors.lastname: Jungmichel
authors.forename: F
authors.initials: F
authors.lastname: Emmrich
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000503:physiopathology*; D002358:Cartilage, Articular / Q000473:pathology*; D017690:Cell Transplantation; D004195:Disease Models, Animal; D006801:Humans; D006824:Hybridization, Genetic; D007150:Immunohistochemistry; D007719:Knee Joint / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D013583:Synovial Membrane / Q000166:cytology / Q000503:physiopathology* / Q000637:transplantation*; D016378:Tissue Transplantation; D014183:Transplantation, Heterologous