Uptake and phosphorylation of thiamine in rabbit primary proximal tubule cells and Madin Darby canine kidney cells. II. Effect of ethanol.
Uptake of [14C]thiamine was studied in renal primary proximal tubule cells and in Madin Darby Canine Kidney cells in culture. Findings were compared with data for the accumulation of [14C]thiamine and its phosphorylation in renal cortical slices. There was a saturable component for thiamine uptake in all cell types. When normalized for milligrams of protein, renal cortical slices accumulated 82% less 14C than renal primary proximal tubule cells and 51% less than distal tubule cells. Maximal [14C]thiamine levels accumulated by the saturable component was 1.33 nmol/g in slices and 7.02 nmol/g in proximal tubule cells. Ethanol, at 25 mM, inhibited thiamine uptake and phosphorylation in the cell cultures similar to its effect in the kidney tissue.
Mahajan
M A
MA
Department of Pharmacology and Therapeutics, School of Medicine and Biomedical Sciences, State University of New York at Buffalo.
Acara
M
M
Taub
M
M
D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D000431:Ethanol / Q000494:pharmacology*; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009943:Organophosphorus Compounds / Q000378:metabolism; D010766:Phosphorylation; D011817:Rabbits; D013831:Thiamine / Q000378:metabolism*
D009943:Organophosphorus Compounds; D000431:Ethanol; D013831:Thiamine