title: Complete cDNA encoding human phospholipid transfer protein from human endothelial cells.
abstract: Phospholipid transfer protein, with an apparent molecular mass of 81 kDa, was purified from human plasma. The NH2-terminal amino acid sequence of a 51-kDa proteolytic fragment obtained from phospholipid transfer protein allowed degenerate primers to be designed for polymerase chain reaction and the eventual isolation of a full-length cDNA from a human endothelial cDNA library. The cDNA is 1,750 base pairs in length and contains an open reading frame of 1,518 nucleotides encoding a leader of 17 amino acids and a mature protein of 476 residues. Northern blot analysis shows a single mRNA transcript of approximately 1.8 kilobases with a wide tissue distribution. The gene was mapped to chromosome 20 using a human/rodent somatic cell hybrid mapping panel. Phospholipid transfer protein was found to be homologous to human cholesteryl ester transfer protein, human lipopolysaccharide-binding protein, and human neutrophil bactericidal permeability increasing protein (20, 24, and 26% identity, respectively).
authors.affiliation: Department of Medicine, University of Washington, Seattle 98103.
authors.forename: J R
authors.initials: JR
authors.lastname: Day
authors.forename: J J
authors.initials: JJ
authors.lastname: Albers
authors.forename: C E
authors.initials: CE
authors.lastname: Lofton-Day
authors.forename: T L
authors.initials: TL
authors.lastname: Gilbert
authors.forename: A F
authors.initials: AF
authors.lastname: Ching
authors.forename: F J
authors.initials: FJ
authors.lastname: Grant
authors.forename: P J
authors.initials: PJ
authors.lastname: O'Hara
authors.forename: S M
authors.initials: SM
authors.lastname: Marcovina
authors.forename: J L
authors.initials: JL
authors.lastname: Adolphson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004730:Endothelium, Vascular / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger