title: A new chronometric assay to determine plasma antifactor Xa activity which is insensitive to the antithrombin activity of low molecular weight heparins.
abstract: Some commercially available chronometric assays are influenced by the residual antithrombin activity of low molecular weight heparins (LMWH) and they underestimate the ex vivo anti Xa activity. We have evaluated a new kit (Staclot-Heparin) highly specific for the anti Xa activity of LMWH. A comparison with the results given by a reference chromogenic method (Stachrom-Heparin) indicates a very good correlation between the 2 assays (r = 0.95, n = 59). This new assay is not influenced by vitamin K antagonist treatments. Clinical biologists now have the possibility of determining the anti Xa activity generated by LMWH easily and accurately, using a chronometric assay.
authors.affiliation: Laboratoire d'Hémostase, Hôpital de Purpan, Toulouse, France.
authors.forename: M
authors.initials: M
authors.lastname: Dignac
authors.forename: A M
authors.initials: AM
authors.lastname: Gabaig
authors.forename: J P
authors.initials: JP
authors.lastname: Cambus
authors.forename: B
authors.initials: B
authors.lastname: Boneu
mesh_terms: D000906:Antibodies / Q000097:blood*; D000991:Antithrombins / Q000494:pharmacology*; D002863:Chromogenic Compounds; D002909:Chronobiology Phenomena; D015951:Factor Xa / Q000276:immunology*; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D006801:Humans
chemical_list: D000906:Antibodies; D000991:Antithrombins; D002863:Chromogenic Compounds; D006495:Heparin, Low-Molecular-Weight; D015951:Factor Xa