title: Interleukin-1 beta stimulates prostaglandin E2 and F2 alpha synthesis in human ovarian granulosa cells in culture.
abstract: To evaluate the roles of interleukin-1 (IL-1) in regulation of ovarian prostaglandin (PG) synthesis, we examined the effects of IL-1 beta on PGE2, PGE2 alpha, 6-keto-PGF 1 alpha and thromboxane (TX) B2 synthesis in cultures of human ovarian granulosa cells. Granulosa cells were obtained from hyperstimulated follicles in patients undergoing oocyte retrieval for in vitro fertilization-embryo transfer (IVF-ET). IL-1 beta increased immunoreactive concentrations of PGE2 and PGF2 alpha in culture medium in time- and dose-dependent manners. Concentration of PGE2 was significantly higher after 24 h incubation with 5 or more units/ml of IL-1 beta, when compared to the control value obtained without IL-1 beta (P < 0.05). Concentration of PGF2 alpha was significantly higher after 8 h incubation with more than 2 units/ml of IL-1 beta (P < 0.05). The increase in PGE2 was observed even in the presence of human chorionic gonadotropin (hCG), and blocked by indomethacin, an inhibitor of cyclooxygenase. During a 10 day incubation period, stimulatory effects of IL-1 beta on PG synthesis were observed only on the first 2 days incubations. Concentrations of 6-keto-PGF 1 alpha and TXB2 were below our measurement limits. This study demonstrated that IL-1 beta stimulates PG synthesis in human ovarian granulosa cells in vitro. IL-1 seems to play an important role in regulating ovarian functions.
authors.affiliation: Department of Obstetrics and Gynecology, Miyazaki Medical College, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Nagai
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Ikenoue
authors.forename: N
authors.initials: N
authors.lastname: Mori
mesh_terms: D002478:Cells, Cultured; D015237:Dinoprost / Q000096:biosynthesis*; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology* / Q000502:physiology; D007700:Kinetics; D010053:Ovary / Q000502:physiology; D010060:Ovulation / Q000502:physiology
chemical_list: D007375:Interleukin-1; D015237:Dinoprost; D015232:Dinoprostone