The neurobehavioural toxicity of endosulfan in rats: a serotonergic involvement in learning impairment.
Oral administration of the insecticide endosulfan (2 mg/kg per day) for 90 days in immature male rats resulted in an inhibition of pole-climbing escape response to electric shock (unconditioned) and avoidance response to buzzer (conditioned). These responses reflect respectively their learning and memory processes. The escape response but not the avoidance response was reinstated significantly by the 5-hydroxytryptamine (5-HT) depletor, p-chlorophenylalanine (PCPA, 100 mg/kg per day for 3 days). Endosulfan increased 5-HT concentrations in the cerebrum and midbrain regions. Protein content and acetylcholinesterase activity were unaltered in the brain. The spontaneous motor activity of these animals was stimulated. Their muscle coordination on rota-rod apparatus was unaffected. These findings were interpreted as an indication that a motivation deficit and not motor impairment was responsible for the inhibitory action of endosulfan on pole-climbing escape and avoidance responses. Thus, endosulfan was suggested to produce learning and memory deficit. A serotonergic involvement was significant in endosulfan-induced learning impairment and it appeared to be negligible in its memory disrupting action.
Paul
V
V
Department of Pharmacology and Environmental Toxicology, Dr. A.L.M. Post Graduate Institute of Basic Medical Sciences, University of Madras, India.
Balasubramaniam
E
E
Kazi
M
M
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects*; D001923:Brain Chemistry / Q000187:drug effects*; D004726:Endosulfan / Q000633:toxicity*; D004924:Escape Reaction / Q000187:drug effects; D007859:Learning Disabilities / Q000139:chemically induced*; D008297:Male; D011506:Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000032:analysis / Q000378:metabolism*
D011506:Proteins; D012701:Serotonin; D000110:Acetylcholinesterase; D004726:Endosulfan