title: Inducible amber suppressor for Bacillus subtilis.
abstract: An amber suppressor variant of Bacillus subtilis tyrosyl-tRNA was constructed and placed under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible Pspac promoter. Addition of IPTG resulted in a 50-fold increase in the expression of an rpsD-lacZ fusion containing a UAG amber codon. This system permitted isolation of a conditional lethal mutant which required IPTG for growth.
authors.affiliation: Department of Biochemistry and Molecular Biology, Albany Medical College, New York 12208.
authors.forename: F J
authors.initials: FJ
authors.lastname: Grundy
authors.forename: T M
authors.initials: TM
authors.lastname: Henkin
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D003062:Codon; D004790:Enzyme Induction / Q000187:drug effects; D005804:Genes, Lethal / Q000235:genetics; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012365:RNA, Transfer, Tyr / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013489:Suppression, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D003062:Codon; D012365:RNA, Transfer, Tyr; D011993:Recombinant Fusion Proteins; D007544:Isopropyl Thiogalactoside; D001616:beta-Galactosidase