[Interaction of human serum albumin with bilirubin in solution and in suspensions containing bile pigment of membranous particles].
Spectrophotometrically measured in homogeneous aqueous medium association constants for the formation of bilirubin--human human serum albumin complex did not correlate with the intensity of the same complex formation as a result of bilidiene release from nerve cell plasma membrane particles containing biliary pigment in aqueous medium during changed hydrogen parameter and ionic strength of the medium (pH22 degrees 6.8, 7.2, or 7.5 and NaCl concentration in the medium 0.135, 0.150, or 0.165 M), such changes being compatible with normal mammalian cellular and tissue vital activity, the rest conditions being compatible. Study of the time course of the said complex formation in membranous particle suspension seems to be more adequate and therefore more reliable for organic anions (e.g. bilirubin) transport related assessment of detoxifying activity of infused human serum albumin preparations than measurement of the said association constants in a homogeneous aqueous medium.
Tkachenko
A V
AV
D000818:Animals; D001654:Bile Pigments / Q000737:chemistry*; D001663:Bilirubin / Q000737:chemistry*; D002462:Cell Membrane; D006801:Humans; D051381:Rats; D012709:Serum Albumin / Q000737:chemistry*; D012996:Solutions; D013574:Synaptosomes / Q000648:ultrastructure*
D001654:Bile Pigments; D012709:Serum Albumin; D012996:Solutions; D001663:Bilirubin
Vzaimodeĭstvie syvorotochnogo al'bumina cheloveka s bilirubinom v rastvore i v suspenzii soderzhashchikh zhelchnyĭ pigment membrannykh chastits.