Expression of alpha-1,3 linkage-containing oligomannosyl residues in a cell-wall mannan of Candida tropicalis grown in yeast extract-Sabouraud liquid medium under acidic conditions.
We investigated the cell-wall mannan obtained from Candida tropicalis IFO 1647 strain cells grown in yeast extract-Sabouraud medium at pH 3.0 by two-dimensional homonuclear Hartmann-Hahn spectroscopy. The results indicate that the phosphate group and the side chains containing a beta-1,2-linked mannopyranose unit decreased compared to those of mannan from cells grown under conventional conditions (pH 5.9) with concomitant expression of alpha-1,3 linkage-containing oligomannosyl side chains. The results of acetolysis of these mannans indicated that the presence of alpha-1,3-linked mannopyranose unit existed in side chains corresponding to pentaose and hexaose, Manp alpha 1-3 Manp alpha 1-2Manp alpha 1-2 Manp alpha 1-2Man, and Manp alpha 1-2Manp alpha 1-3Manp alpha 1-2Manp alpha 1-2Manp alpha 1-2Man, in the mannan from cells grown at pH 3.0.
Kobayashi
H
H
Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.
Shibata
N
N
Suzuki
A
A
Takahashi
S
S
Suzuki
M
M
Matsuda
K
K
Hisamichi
K
K
Suzuki
S
S
D002175:Candida / Q000737:chemistry* / Q000254:growth & development; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008351:Mannans / Q000737:chemistry*; D008358:Mannose / Q000032:analysis*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis
D003470:Culture Media; D008351:Mannans; D009844:Oligosaccharides; D008358:Mannose