title: Matrix-assisted laser desorption/ionization of restriction enzyme-digested DNA.
abstract: Matrix-assisted laser desorption/ionization, by a time-of-flight mass spectrometer, has been successfully used for detection of restriction enzyme-digested DNA. However, the oligonucleotide segments detected correspond to the molecular weights of single strands.
authors.affiliation: Oak Ridge National Laboratory, Tennessee 37831.
authors.forename: K
authors.initials: K
authors.lastname: Tang
authors.forename: S L
authors.initials: SL
authors.lastname: Allman
authors.forename: C H
authors.initials: CH
authors.lastname: Chen
authors.forename: L Y
authors.initials: LY
authors.lastname: Cháng
authors.forename: M
authors.initials: M
authors.lastname: Schell
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded / Q000032:analysis; D006868:Hydrolysis; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D016133:Polymerase Chain Reaction
chemical_list: D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes