title: Changes in the fatty acid composition of stored erythrocytes from sheep of different ages.
abstract: This study examined the profile in sheep erythrocyte fatty acids from animals of different ages during storage at 4 degrees C in a nutritive medium for up to 6 days. The changes found in the fatty acyl profile were a decrease (P < 0.01) in the percentage of arachidonic acid and an increase (P < 0.01) in the percentage of minor fatty acids (representing < 2% in each case; 20:2, 22:0 and 22:1) with respect to fresh erythrocytes in all age groups. However, the saturated/unsaturated ratios and unsaturated index started almost constant in all cases. The changes observed occurred after 24-48 h of storage, with significant increases (P < 0.01) in the fluorescence detected in the lipid extracts from stored erythrocytes during this period. The above findings suggest peroxidative damage and changes in the erythrocyte lipid membrane during storage.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.forename: J
authors.initials: J
authors.lastname: Sánchez-Yagüe
authors.forename: E M
authors.initials: EM
authors.lastname: Martín-Valmaseda
authors.forename: A
authors.initials: A
authors.lastname: Felipe
authors.forename: J A
authors.initials: JA
authors.lastname: Cabezas
authors.forename: M
authors.initials: M
authors.lastname: Llanillo
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D002849:Chromatography, Gas; D004912:Erythrocytes / Q000737:chemistry* / Q000502:physiology; D005227:Fatty Acids / Q000737:chemistry* / Q000502:physiology; D008055:Lipids / Q000737:chemistry / Q000502:physiology; D012756:Sheep / Q000502:physiology
chemical_list: D005227:Fatty Acids; D008055:Lipids