title: Glycogen turnover during refeeding in the postabsorptive dog: implications for the estimation of glycogen formation using tracer methods.
abstract: Recent 13C nuclear magnetic resonance (13C-NMR) studies in the anesthetized rat and perfused liver suggest that hepatic glycogen is simultaneously synthesized and degraded, even during combined hyperglycemia and hyperinsulinemia. The presence of glycogen turnover would confound efforts to study glycogen repletion with the use of tracer methods during feeding, particularly if the liver is not glycogen-depleted. To ascertain whether glycogen turnover occurs during normal feeding, we measured liver uptake of glucose in 10 awake, healthy, postabsorptive dogs with long-term arterial, portal, and hepatic venous catheters before and for 3 hours after a meal of either glucose alone (1.5 g/kg) or glucose supplemented with crystalline amino acids (0.7 g/kg); the meal was labeled with D-[3-3H]glucose and [U-14C]alanine. Liver glycogen level was measured in biopsies obtained before and at 180 minutes after the meal. The postabsorptive liver glycogen content was 4.3 +/- 0.9 g/100 g, and net hepatic glucose release averaged 1.8 +/- 0.3 mg/min/kg. Over the 3 hours following feeding, the liver took up glucose (0.37 +/- 0.14 and 0.33 +/- 0.16 g/kg body weight in dogs receiving glucose and glucose with amino acids, respectively). At 3 hours, glycogen synthesis from D-[3-3H]glucose in the two groups averaged 0.24 +/- 0.09 and 0.22 +/- 0.05 g/kg, or approximately 15% of the ingested glucose load. 14C-glucose also was found in liver glycogen, demonstrating ongoing hepatic gluconeogenesis.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville 22908.
authors.forename: E J
authors.initials: EJ
authors.lastname: Barrett
authors.forename: S
authors.initials: S
authors.lastname: Bevilacqua
authors.forename: R A
authors.initials: RA
authors.lastname: DeFronzo
authors.forename: E
authors.initials: E
authors.lastname: Ferrannini
mesh_terms: D000284:Administration, Oral; D000409:Alanine / Q000008:administration & dosage / Q000378:metabolism; D000818:Animals; D001706:Biopsy; D001786:Blood Glucose / Q000032:analysis; D002250:Carbon Radioisotopes; D004285:Dogs; D004435:Eating / Q000502:physiology*; D005215:Fasting / Q000502:physiology; D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D008099:Liver / Q000473:pathology; D008112:Liver Glycogen / Q000032:analysis / Q000378:metabolism*; D008297:Male; D013997:Time Factors; D014316:Tritium
chemical_list: D001786:Blood Glucose; D002250:Carbon Radioisotopes; D008112:Liver Glycogen; D014316:Tritium; D005947:Glucose; D000409:Alanine