title: Molecular organisation and expression pattern of the segment polarity gene fused of Drosophila melanogaster.
abstract: The Drosophila segment-polarity gene fused (fu) is required for pattern formation within embryonic segments and imaginal discs. We previously reported that the 5' part of the fused gene is homologous to the catalytic domain of serine/threonine kinases. We present here the sequence of the complete transcription unit, which predicts a 805 amino acid long protein. The kinase domain actually corresponds to 268 amino acids in the N-terminal part, and no known function can be attributed to the rest of the putative FUSED protein. Transcripts from the fused gene have been characterized: a unique 3.2 kb fused transcript is produced in nurse cells, in low abundance, from stage 8 of oogenesis, and persistently through the rest of oogenesis. In embryos, this transcript is evenly distributed in all embryonic cells until the extended germ band stage, after which its amount strongly decreases. Ubiquitous expression is detected later in imaginal wing and leg discs. Possible roles of the FUSED protein in signal transduction pathways required for intercellular communication at different stages of development are discussed.
authors.affiliation: Centre de Génétique Moléculare du C.N.R.S., Gif sur Yvette, France.
authors.forename: P
authors.initials: P
authors.lastname: Therond
authors.forename: D
authors.initials: D
authors.lastname: Busson
authors.forename: E
authors.initials: E
authors.lastname: Guillemet
authors.forename: B
authors.initials: B
authors.lastname: Limbourg-Bouchon
authors.forename: T
authors.initials: T
authors.lastname: Preat
authors.forename: R
authors.initials: R
authors.lastname: Terracol
authors.forename: H
authors.initials: H
authors.lastname: Tricoire
authors.forename: C
authors.initials: C
authors.lastname: Lamour-Isnard
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D016678:Genome; D006720:Homozygote; D008297:Male; D010641:Phenotype