Ethanol and acetaldehyde metabolism in cultured rat hepatocytes.
In the present study, ethanol (Et-OH) and acetaldehyde (Ac-CHO) metabolism in primary cultured hepatocytes isolated from chronically alcohol-fed rats were analysed to elucidate the characteristics of the metabolism of Ac-CHO produced through the non-alcohol dehydrogenase (non-ADH) pathway. Dimethyl sulfoxide was added to the culture medium to prevent a decrease in P450 IIE1 activity. Ac-CHO formed in the culture medium was trapped by semicarbazide to prevent a reutilization of Ac-CHO by hepatocytes. The degradation rate of Ac-CHO in the liver was similar in alcohol and non-alcohol-treated rats. Therefore, the characteristic of the metabolism of Ac-CHO in the chronically alcohol-fed rats was not clarified in this study. The possibility that the Ac-CHO trapping by semicarbazide was only 70%, and that the remaining Ac-CHO was reutilized for oxidation in the hepatocytes, should be considered. Some method to trap Ac-CHO completely in the culture medium is needed to clarify the characteristics of Ac-CHO metabolism.
Ueshima
Y
Y
Department of Internal Medicine, Kanazawa Medical University, Ishikawa, Japan.
Matsuda
Y
Y
Wang
B Y
BY
Takase
S
S
Takada
A
A
D000079:Acetaldehyde / Q000378:metabolism*; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000437:Alcoholism / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D019392:Cytochrome P-450 CYP2E1; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D008297:Male; D006899:Mixed Function Oxygenases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012663:Semicarbazides
D003470:Culture Media; D012663:Semicarbazides; C010059:carbamylhydrazine; D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D000426:Alcohol Dehydrogenase; D019392:Cytochrome P-450 CYP2E1; D000444:Aldehyde Dehydrogenase; D000079:Acetaldehyde