Expression of functional protease and subviral particles by vaccinia virus containing equine infectious anaemia virus gag and 5' pol genes.
Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gag gene (VGag) or gag plus the 5' pol encoding protease (VGag/PR) were evaluated with monoclonal antibody to a p26 capsid protein linear epitope (QEISKFLTD). Both recombinant viruses expressed Gag precursor protein (55K) whereas only VGag/PR expressed a detectable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.
McGuire
T C
TC
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University.
O'Rourke
K I
KI
Baszler
T V
TV
Leib
S R
SR
Brassfield
A L
AL
Davis
W C
WC
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D015685:Fusion Proteins, gag-pol / Q000096:biosynthesis / Q000235:genetics*; D015750:Genes, gag / Q000235:genetics; D015751:Genes, pol / Q000235:genetics; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D007668:Kidney / Q000382:microbiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational / Q000235:genetics; D011533:Proviruses; D012367:RNA, Viral / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D014616:Vaccinia virus / Q000235:genetics*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015685:Fusion Proteins, gag-pol; D012367:RNA, Viral; D011994:Recombinant Proteins; D010450:Endopeptidases