title: High levels of portal TNF-alpha during abdominal aortic surgery in man.
abstract: During shock or multiple organ dysfunction syndrome, translocation of bacteria and/or lipopolysaccharide (LPS) from the ischaemic gut might occur and could explain the excess of cytokine production detectable in plasma. To test this hypothesis, we studied a model of mild gut ischaemia due to bowel manipulation and aortic clamping in patients undergoing abdominal aortic surgery (n = 14). Per-operative levels of LPS and cytokines were measured before clamping and after reperfusion, and compared in systemic and portal blood. Systemic levels of LPS and cytokines were measured in a control group of patients undergoing internal carotid surgery (n = 7). Portal LPS was detectable (i.e., > 12 pg/ml) in 36% of the patients undergoing aortic surgery after bowel manipulation, and in 71% after clamp release. Similar levels of LPS were observed in portal and systemic blood after clamp release. Circulating tumour necrosis factor alpha (TNF-alpha) was observed in all patients undergoing aortic surgery. Levels of portal TNF-alpha were higher than those in systemic blood after bowel manipulation as well as after reperfusion (P = 0.02 and 0.007, respectively). LPS was never detected in control patients and TNF-alpha was detectable in only two out of seven patients. Mean levels of IL-6 were similar in the two groups, with a peak on the day following surgery, confirming that circulating IL-6 is associated with any surgical procedures. Our data indicate that bowel manipulation, aortic clamping and reperfusion lead to similar levels of portal and systemic circulating LPS.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Unité d'Immuno-Allergie, Institut Pasteur, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Cabiè
authors.forename: J C
authors.initials: JC
authors.lastname: Farkas
authors.forename: C
authors.initials: C
authors.lastname: Fitting
authors.forename: C
authors.initials: C
authors.lastname: Laurian
authors.forename: J M
authors.initials: JM
authors.lastname: Cormier
authors.forename: J
authors.initials: J
authors.lastname: Carlet
authors.forename: J M
authors.initials: JM
authors.lastname: Cavaillon
mesh_terms: D000368:Aged; D001012:Aorta, Abdominal / Q000601:surgery*; D004064:Digestive System / Q000098:blood supply*; D005500:Follow-Up Studies; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D007431:Intraoperative Complications; D007511:Ischemia / Q000209:etiology*; D008070:Lipopolysaccharides / Q000097:blood*; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D011168:Portal System / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha