[Stimulation of respiration in liver mitochondria by glucose in the absence of hexokinase activity. The effect of saccharose and several inhibitors of oxidative phosphorylation].
The effect of glucose on rat liver mitochondrial respiration after elimination of possible phosphorylation by mitochondrial hexokinase has been studied. It has been found that during isolation of mitochondria without washing and their subsequent washing with KCl-media glucose stimulates state 3 respiration but has no effect on the rate of state 4 respiration or on the uncoupler (2.4-dinitrophenol)-mediated respiration. The half-maximum effect is observed at the glucose concentration of about 2.4 mM. Washing of mitochondria with KCl or their isolation in a sucrose-containing medium without washing eliminates the stimulating effect of glucose. The sucrose present in the incubation medium suppresses the stimulating effect of glucose in a competitive manner. Oligomycin removes the stimulating effect when used at concentrations causing the suppression of state 3 mitochondrial respiration by 20%. Under these conditions carboxyatracytyloside markedly enhances the glucose effect. It is concluded that glucose controls the oxidative phosphorylation, acting as an adenine nucleotide translocator.
Samartsev
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D019297:2,4-Dinitrophenol; D000818:Animals; D004140:Dinitrophenols / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D051381:Rats; D013395:Sucrose / Q000494:pharmacology*; D014475:Uncoupling Agents / Q000494:pharmacology*
D004140:Dinitrophenols; D009840:Oligomycins; D014475:Uncoupling Agents; D013395:Sucrose; D006593:Hexokinase; D005947:Glucose; D019297:2,4-Dinitrophenol
Stimuliatsiia gliukozoĭ dykhaniia v mitokhondriiakh pecheni v otsutstvie aktivnosti geksokinazy. Vliianie sakharozy i nekotorykh ingibitorovokislitel'nogo fosforilirovaniia.