Usability of the hypoosmotic swelling "Water-test"--a simple method to assess sperm membrane integrity in mouse spermatozoa.
The functional integrity of the membrane of sperm from 3 strains of mice (BALB/c, CBA/KW, and C57BL/KW) was assessed by studying the swelling reaction of the sperm when suspended in a medium of distilled water. The results were correlated with analysis of sperm motility and those of Eosin-Y staining. The results obtained from the Eosin-Y test and "Water-test" differed (P < 0.01) between strains C57BL/KW and both BALB/C, and CBA/KW. A good correlation was observed in all strains of mice between the values of the "Water-test" and those for Eosin-Y staining (P < 0.01).
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Department of Embryology and Medical Genetics, Collegium Medicum, Jagiellonian University, Kraków, Poland.
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability; D008297:Male; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013094:Spermatozoa / Q000502:physiology*; D014882:Water-Electrolyte Balance