title: Reconstruction of glucose uptake and phosphorylation in a glucose-negative mutant of Escherichia coli by using Zymomonas mobilis genes encoding the glucose facilitator protein and glucokinase.
abstract: Expression of the Zymomonas mobilis glf (glucose facilitator protein) and glk (glucokinase) genes in Escherichia coli ZSC113 (glucose negative) provided a new functional pathway for glucose uptake and phosphorylation. Both genes were essential for the restoration of growth in glucose minimal medium and for acid production on glucose-MacConkey agar plates.
authors.affiliation: Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
authors.forename: J L
authors.initials: JL
authors.lastname: Snoep
authors.forename: N
authors.initials: N
authors.lastname: Arfman
authors.forename: L P
authors.initials: LP
authors.lastname: Yomano
authors.forename: R K
authors.initials: RK
authors.lastname: Fliege
authors.forename: T
authors.initials: T
authors.lastname: Conway
authors.forename: L O
authors.initials: LO
authors.lastname: Ingram
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D005941:Glucokinase / Q000235:genetics*; D005947:Glucose / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010766:Phosphorylation; D017355:Zymomonas / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C086512:glucose facilitator protein, Zymomonas; D005941:Glucokinase; D005947:Glucose