title: Molecular species of choline and ethanolamine glycerophospholipids in rat brain myelin during development.
abstract: The composition of the molecular species of various phospholipid subclasses was examined in myelin isolated from brain of 15-, 21- and 90-day-old rats. The molecular species of diacylglycerophosphocholine (PtdCho), diacylglycerophosphoethanolamine (PtdEtn) and plasmenyl-ethanolamine (PlsEtn) were quantified by high-performance liquid chromatography (HPLC) after phospholipase C treatment and dinitrobenzoyl derivatization. In rat brain myelin, each phospholipid subclass showed a specific pattern of molecular species that changed during development. PtdCho contained large amounts of saturated/monounsaturated and disaturated species and low amounts of saturated/polyunsaturated species. During brain development, the levels of saturated/monounsaturated molecular species increased whereas those of the disaturated and saturated/polyunsaturated species decreased. PtdEtn were characterized by their low levels of disaturated species and a high content of saturated/monounsaturated and saturated/polyunsaturated species, of which those containing fatty acids of the n-3 series decreased, whereas those containing fatty acids of the n-6 series did not change during brain development. The levels of saturated/monounsaturated species increased in PtdEtn. No disaturated molecular species could be detected in PlsEtn. This alkenylacyl subclass contained large amounts of saturated/polyunsaturated, saturated/monounsaturated and dimonounsaturated molecular species. During development, the levels of saturated/polyunsaturated molecular species decreased while those of the two others increased. The data indicated that myelin sheaths undergo phospholipid changes during brain development and maturation.
authors.affiliation: Centre de Neurochimie du CNRS, Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, Strasbourg, France.
authors.forename: C
authors.initials: C
authors.lastname: Leray
authors.forename: L L
authors.initials: LL
authors.lastname: Sarliève
authors.forename: H
authors.initials: H
authors.lastname: Dreyfus
authors.forename: R
authors.initials: R
authors.lastname: Massarelli
authors.forename: L
authors.initials: L
authors.lastname: Binaglia
authors.forename: L
authors.initials: L
authors.lastname: Freysz
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D009186:Myelin Sheath / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D051381:Rats
chemical_list: D005227:Fatty Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines