Simultaneous determination of hydroperoxides of phosphatidylcholine, cholesterol esters and triacylglycerols by column-switching high-performance liquid chromatography with a post-column detection system.
A method for the simultaneous determination of hydroperoxides of phosphatidylcholines (PC), triacylglycerols (TG) and cholesterol esters (CE) has been developed. A sample was separated into a combined TG and CE hydroperoxides fraction and a PC hydroperoxides fraction on a short silica column. The fractions were introduced into an ODS column and another silica column by a valve-switching device. The PC hydroperoxides were monitored by a post-column detection system with diphenyl-1-pyrenylphosphine, and the TG and CE hydroperoxides were monitored by another switching device. With this system, the hydroperoxides were determined at the picomole level within 32 min. Their detection limits were 2-4 pmol at a signal-to-noise ratio of 3, and the relative standard deviations of the peak areas were 1.6-3.1%. This method was successfully applied to determine lipid hydroperoxides in human plasma.
Akasaka
K
K
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Ohrui
H
H
Meguro
H
H
D002784:Cholesterol / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D008054:Lipid Peroxides / Q000032:analysis* / Q000097:blood; D008297:Male; D009943:Organophosphorus Compounds; D010713:Phosphatidylcholines / Q000032:analysis* / Q000097:blood; D011721:Pyrenes; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014280:Triglycerides / Q000032:analysis* / Q000097:blood
D007202:Indicators and Reagents; D008054:Lipid Peroxides; D009943:Organophosphorus Compounds; D010713:Phosphatidylcholines; D011721:Pyrenes; D014280:Triglycerides; C057590:phosphatidylcholine hydroperoxide; C066322:diphenyl-1-pyrenylphosphine; C002843:cholesterol hydroperoxide; D002784:Cholesterol