[Quantitative analysis of the binding of peptides to HLA molecules role of polymolphic HLA molecules in the immune response].
We established HLA-peptide binding assay system, using biotinylated peptide and HLA class II transfectants to make clear the interaction of peptides and HLA molecules. By using this system and T cell proliferation assay, we analyzed the effect of polymorphism of DR4 subtypes on responding to M12(4) peptide which derived from M12 protein of Streptococcus. We found amino acid residue which determines T cell recognition is DR beta-74 (Glutamate to Alanine) on this peptide. Using this system, we expect to understand the mechanisms of immune response associated with HLA class II molecules.
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Department of Genetics, Kyushu University, Fukuoka.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D015804:HLA-DR4 Antigen / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D011110:Polymorphism, Genetic; D013601:T-Lymphocytes / Q000276:immunology*
D001426:Bacterial Proteins; D015804:HLA-DR4 Antigen; D010455:Peptides