Genetic diversity of the iron-binding protein (Fbp) gene of the pathogenic and commensal Neisseria.
The pathogenic Neisseria and most commensal Neisseria species produce an iron-binding protein (Fbp) when grown under iron-limited conditions. In the current study, we confirmed the presence of Fbp, as well as DNA sequences homologous to the gonococcal fbp, in strains of N. gonorrhoeae, N. meningitidis, N. cinerea, N. lactamica, N. subflava, N. kochii and N. polysaccharea. The fbp genes from these strains were amplified by the polymerase chain reaction, digested with StuI or RsaI, and the restriction patterns examined. The patterns for the gonococcal and meningococcal fbp were virtually identical; however, variations were observed in the fbp sequences of the commensal Neisseria species. N. flavescens, N. mucosa, N. sicca, N. ovis and Branhamella catarrhalis, did not produce Fbp as detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reactivity with an Fbp specific monoclonal antibody, nor did they hybridize to an fbp-specific DNA probe.
Genco
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Department of Microbiology and Immunology, Morehouse School of Medicine, Atlanta, Georgia 30310.
Berish
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Chen
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Morse
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Trees
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D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D033862:Iron-Binding Proteins; D008969:Molecular Sequence Data; D009343:Neisseria / Q000235:genetics*; D009344:Neisseria gonorrhoeae / Q000235:genetics; D009345:Neisseria meningitidis / Q000235:genetics; D033902:Periplasmic Binding Proteins; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017421:Sequence Analysis; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D033862:Iron-Binding Proteins; D033902:Periplasmic Binding Proteins