Identification of nodulation genes (nod locus 5) from Azorhizobium caulinodans ORS571.
An 800 bp AccI-PUCII DNA containing promoter region of nodABC (P1) from A. caulinodans ORS571 was cloned and used as a hybridization probe against the chromosome DNA, which led to the identification of another 8.4-kb EcoRI fragment showing homology to P1. A corresponding clone of 8.4-kb DNA was isolated from a pLAFI gene bank (pRG90). The restriction enzyme analysis and DNA-DNA hybridization of 8.4-kb DNA indicated the P1 homology was located in the 450-bp SmaI-SphI region (P2 region). omega insertion deletion of 8.4 kb DNA resulted in a mutant strain ORS571-5 that delayed to nodulate the stems of S. rostrata. This phenotype was complemented by the introduction of pRK84 carrying nod locus 5 DNA.
Gao
M S
MS
Department of Biotechnology, IAAE, Chinese Academy of Agricultural Sciences, Beijing.
You
C B
CB
Goethals
K
K
Holsters
M
M
D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012230:Rhizobiaceae / Q000235:genetics*
D015342:DNA Probes