Characterization of a temperate phage hosted by Alcaligenes eutrophus strain A5.
Nineteen strains of Alcaligenes eutrophus were tested for the presence of prophages. One strain that lysed upon mitomycin C treatment produced a phage which could not form plaques on any of the strains available. DNA extracted from partially purified phage lysates was digested with various restriction enzymes which showed that the 42 kb long viral double-stranded DNA circularizes by means of cohesive ends. To our knowledge, this is the first description of a phage for the genus Alcaligenes.
Faelen
M
M
Laboratoire de Génétique, Université Libre de Bruxelles, Rhode St Genèse, Belgium.
Merlin
C
C
Geuskens
M
M
Mergeay
M
M
Toussaint
A
A
D000421:Alcaligenes / Q000187:drug effects*; D001435:Bacteriophages / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D016685:Mitomycin / Q000494:pharmacology; D015183:Restriction Mapping
D004279:DNA, Viral; D016685:Mitomycin