title: Sequencing and characterization of trypsin modulating oostatic factor (TMOF) from the ovaries of the grey fleshfly, Neobellieria (Sarcophaga) bullata.
abstract: Injection of crude extracts of late vitellogenic ovaries into staged females of the grey fleshfly Neobellieria (Sarcophaga) bullata inhibited oocyte development and biosynthesis of trypsin-like enzymes in the gut. Trypsin synthesis in N. bullata is cyclic and is correlated with egg development, which is discontinuous. A trypsin modulating oostatic factor (Neb-TMOF) was purified from 10,000 vitellogenic ovaries and sequenced by mass spectrometry. Neb-TMOF is a hexapeptide (NH2-NPTNLH-COOH). Injection of the hormone at physiological concentrations (10(-9) M), inhibited trypsin-like synthesis by the midgut of liver-fed female flies, and caused a reduction of the vitellogenin concentration in the hemolymph and of oocyte growth. The role of Neb-TMOF in controlling egg development and the physiological similarities with Aedes-TMOF are discussed.
authors.affiliation: Zoological Institute, Katholieke Universiteit Leuven, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Bylemans
authors.forename: D
authors.initials: D
authors.lastname: Borovsky
authors.forename: D F
authors.initials: DF
authors.lastname: Hunt
authors.forename: J
authors.initials: J
authors.lastname: Shabanowitz
authors.forename: L
authors.initials: L
authors.lastname: Grauwels
authors.forename: A
authors.initials: A
authors.lastname: De Loof
mesh_terms: D000330:Aedes; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004175:Diptera / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007301:Insect Hormones / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D010053:Ovary / Q000737:chemistry; D017421:Sequence Analysis; D014357:Trypsin / Q000096:biosynthesis; D014819:Vitellogenins / Q000096:biosynthesis
chemical_list: D007301:Insect Hormones; D009842:Oligopeptides; D014819:Vitellogenins; C065612:Peptide oostatic hormone; D014357:Trypsin