title: [Search for new products of gene expression in human cardiac muscle. Microsequencing of proteins after two-dimensional electrophoresis].
abstract: Proteins of the human heart muscle were studied using modified two-dimensional electrophoresis. After separation, proteins were electroblotted onto Immobilon P membranes and several protein spots were used for microsequencing analysis. In most cases the proteins analyzed have blocked N-terminal amino acids. In order to study the primary structure of these proteins, hydrolysis in situ by trypsin followed by reversed-phase HPLC and microsequencing of the resulting peptides were performed. Four protein were identified in 8 analyzed fractions, specifically myosin light chain 1 (MLCl-V/sB), fatty-acid binding protein (heart isoform), alpha (B)-crystallin and alpha-tropomyosin. Amino acid sequences of two proteins were not found among human amino acid sequences collected in SWISSPROT bank (v. 21).
authors.forename: A V
authors.initials: AV
authors.lastname: Laptev
authors.forename: S S
authors.initials: SS
authors.lastname: Shishkin
authors.forename: Ts A
authors.initials: TsA
authors.lastname: Egorov
authors.forename: L I
authors.initials: LI
authors.lastname: Kovalev
authors.forename: M N
authors.initials: MN
authors.lastname: Tsvetkova
authors.forename: M A
authors.initials: MA
authors.lastname: Galiuk
authors.forename: A Kh
authors.initials: AKh
authors.lastname: Musaliamov
authors.forename: A S
authors.initials: AS
authors.lastname: Efimochkin
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D017421:Sequence Analysis
vernacular_title: Poisk novykh produktov gennoĭ ékspressii v serdechnoĭ myshtse cheloveka. Mikrosekvenirovanie belkov posle dvumernogo élektroforeza.