Tracking of murine spleen cells in vivo: detection of PKH26-labeled cells in the pancreas of non-obese diabetic (NOD) mice.
Flow cytometry was used to track the in vivo migration of PKH26-labeled donor spleen cells from diabetic NOD mice that were injected into non-diabetic recipient NOD mice. Flow cytometric analysis of recipient mouse tissues revealed that the donor cells were present in the peripheral blood, spleen and lymph nodes 24 h following injection and could still be detected after 28 days. PKH26(+) cells were also detectable in the pancreas 7 days after injection. Phenotypic analysis of the PKH26(+) cells that migrated into these target organs and tissues showed that the major cell population detected was Thy1.2(+) T-lymphocytes, predominantly the Thy1.2(+)/L3T4(+) subpopulation, but Thy1.2(+)/Lyt2(+) cells as well as B220(+) cells (B lymphocytes) were also present.
Beavis
A J
AJ
Schering Plough Research Institute, Department of Immunology, Kenilworth, NJ 07033.
Pennline
K J
KJ
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D002465:Cell Movement; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D016688:Mice, Inbred NOD; D009930:Organic Chemicals; D010179:Pancreas / Q000473:pathology*; D013154:Spleen / Q000473:pathology*; D013601:T-Lymphocytes / Q000502:physiology
D005456:Fluorescent Dyes; D009930:Organic Chemicals; C070080:PKH 26