[A universal method of purifying genetically engineered human somatotropin and its mutants using metallochelating agent affinity chromatography].
A novel scheme for human recombinant somatotropin and its mutant forms isolation which includes ion-exchange and metal chelate affinity chromatography has been developed. A change in the chromatographic behaviour of the isolated proteins depending on the amino acid substitutions has been demonstrated.
Lopatin
S A
SA
Il'ina
A V
AV
Shul'ga
A A
AA
Grinchenko
O S
OS
Varlamov
V P
VP
Skriabin
K G
KG
Kirpichnikov
M P
MP
D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D013006:Growth Hormone / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008670:Metals; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification
D002614:Chelating Agents; D008670:Metals; D011994:Recombinant Proteins; D013006:Growth Hormone
Universal'nyĭ sposob ochistki genno-inzhenernogo somatotropina cheloveka i ego mutantov s ispol'zovaniem metallokhelat affinnoĭ khromatografii.