Simultaneous determination of felbamate and three metabolites in rat and dog plasmas by high-performance liquid chromatography.
An isocratic liquid chromatographic method employing one extraction step and a 150 mm x 4.6 mm I.D. Spherisorb ODS2, 3-microns HPLC column using UV-absorbance detection at 210 nm has been developed for the quantitation of felbamate and three felbamate metabolites in 0.100-ml aliquots of rat and dog plasmas. The linear quantitation range in rat plasma is 0.195-200 micrograms/ml for felbamate; 1.563-200 micrograms/ml for the p-hydroxy metabolite; 0.391-200 micrograms/ml for the 2-hydroxy metabolite; and 0.098-200 micrograms/ml for the monocarbamate metabolite. The linear quantitation range in dog plasma is 0.195-200 micrograms/ml for felbamate; 0.781-200 micrograms/ml for the p-hydroxy metabolite; 0.195-200 micrograms/ml for the 2-hydroxy metabolite; and 0.098-200 micrograms/ml for the monocarbamate metabolite.
Romanyshyn
L A
LA
Department of Biochemistry, Wallace Laboratories, Division of Carter-Wallace Inc., Cranbury, NJ 08512.
Wichmann
J K
JK
Kucharczyk
N
N
Sofia
R D
RD
D000818:Animals; D000927:Anticonvulsants / Q000097:blood* / Q000493:pharmacokinetics; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D000078328:Felbamate; D048448:Phenylcarbamates; D011409:Propylene Glycols / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D000927:Anticonvulsants; D048448:Phenylcarbamates; D011409:Propylene Glycols; D000078328:Felbamate