Immunoreactive adrenomedullin in human plasma.
A specific and sensitive radioimmunoassay for adrenomedullin has been developed. Half-maximal inhibition of binding of radioiodinated adrenomedullin was observed at 4 fmol/tube. The radioimmunoassay recognized the entire adrenomedullin molecule and has little crossreactivity with adrenomedullin fragment peptides. Adrenomedullin-like immunoreactivity was found to circulate in human plasma at considerable concentration (3.3 +/- 0.39 fmol/ml). The immunoreactivity of adrenomedullin was eluted at almost the same position as synthetic adrenomedullin on gel-filtration chromatography and reverse-phase high-performance liquid chromatography, suggesting that circulating adrenomedullin recognized by the present radioimmunoassay is identical or very similar to authentic adrenomedullin. Plasma immunoreactive adrenomedullin significantly increased in patients with hypertension, with a progressive rise proportionate to disease severity.
Kitamura
K
K
First Department of Internal Medicine, Miyazaki Medical College, Japan.
Ichiki
Y
Y
Tanaka
M
M
Kawamoto
M
M
Emura
J
J
Sakakibara
S
S
Kangawa
K
K
Matsuo
H
H
Eto
T
T
D053607:Adrenomedullin; D000959:Antihypertensive Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006973:Hypertension / Q000097:blood; D007106:Immune Sera; D010455:Peptides / Q000097:blood* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*
D000959:Antihypertensive Agents; D007106:Immune Sera; D010455:Peptides; D053607:Adrenomedullin