title: A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae.
abstract: The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the beta-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and susceptible isolates of M. leprae.
authors.affiliation: Unité de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Honoré
authors.forename: E
authors.initials: E
authors.lastname: Perrani
authors.forename: A
authors.initials: A
authors.lastname: Telenti
authors.forename: J
authors.initials: J
authors.lastname: Grosset
authors.forename: S T
authors.initials: ST
authors.lastname: Cole
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics*; D003622:Dapsone / Q000627:therapeutic use; D004352:Drug Resistance, Microbial / Q000235:genetics; D004359:Drug Therapy, Combination; D005798:Genes, Bacterial; D006801:Humans; D015440:Leprosy, Lepromatous / Q000188:drug therapy / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D009166:Mycobacterium leprae / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011515:Prothionamide / Q000627:therapeutic use; D012008:Recurrence; D012293:Rifampin / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D011515:Prothionamide; D003622:Dapsone; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin