title: Effect of addition of dried healthy or diseased parsnip root tissue to a modified AIN-76A diet on cell proliferation and histopathology in the liver, oesophagus and forestomach of male Swiss Webster mice.
abstract: Umbelliferous crop plants, including the parsnip (Pastinaca sativa L.), elaborate enhanced levels of furocoumarins, including psoralens, when subjected to biotic or abiotic stress. These furocoumarins are recognized to lead to phototoxicity. In this study, the effect of these agents, which are present in diseased parsnip root tissue, on the liver and two tissues on the route of entry to the body (the oesophagus and forestomach) were investigated. Young male Swiss Webster mice were fed for approximately 30 days with modified AIN-76A diets containing 32.5% dried healthy, 32.5% apparently healthy or 32.5% fungicide-treated parsnip root tissue, and 8, 16 or 32.5% dried diseased (Phoma complanata-infected) parsnip root tissue. As controls, three modified AIN-76A diets differing in their edible starch-to-sucrose ratios (C1-C3) were administered for an equal time. Dried healthy parsnip root tissue, compared with controls, did not significantly affect any of the indices of cellular proliferation or histopathological parameters that were assessed. Histopathological examination of the oesophagus and forestomach demonstrated no significant changes as a result of feeding any of the diets containing parsnip tissue. In the liver, the highest level (but neither of the two lower levels) of dried diseased parsnip root tissue led to swelling of the cytoplasm in cells surrounding the central vein of hepatic lobules, with consequent compression of the peripheral cells. Using [3H]thymidine radioautography, a dose-related increase in cell labelling with the level of diseased parsnip root tissue was demonstrated in the liver. Compared with control diet C2 only, the extent of [3H]thymidine labelling in the liver was increased in mice receiving apparently healthy parsnip tissue; a slight, not statistically significant, increase was also noted with fungicide-treated parsnip tissue. Increased [3H]thymidine labelling with the feeding of diseased parsnip tissue was also found in the greater curvature of the forestomach and the region of the oesophageal-forestomach junction, but not at the glandular junction of the forestomach nor in the mid-oesophagus.
authors.affiliation: Nutrition Research Division, Bureau of Nutritional Sciences, Health Canada, Ottawa, Ontario.
authors.forename: R
authors.initials: R
authors.lastname: Mongeau
authors.forename: R
authors.initials: R
authors.lastname: Brassard
authors.forename: R
authors.initials: R
authors.lastname: Cerkauskas
authors.forename: M
authors.initials: M
authors.lastname: Chiba
authors.forename: E
authors.initials: E
authors.lastname: Lok
authors.forename: E A
authors.initials: EA
authors.lastname: Nera
authors.forename: P
authors.initials: P
authors.lastname: Jee
authors.forename: E
authors.initials: E
authors.lastname: McMullen
authors.forename: D B
authors.initials: DB
authors.lastname: Clayson
mesh_terms: D000821:Animal Feed / Q000633:toxicity*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004032:Diet; D004947:Esophagus / Q000187:drug effects* / Q000473:pathology; D011564:Furocoumarins / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D009469:Neuromuscular Junction / Q000187:drug effects; D010935:Plant Diseases; D010944:Plants / Q000737:chemistry*; D013270:Stomach / Q000187:drug effects* / Q000473:pathology
chemical_list: D011564:Furocoumarins