[Monoclonal antibodies in blood group serology. Part 1: Technique of production, ABO and Lewis system].
OBJECTIVE
The introduction of monoclonal antibodies in routine blood group typing has also given rise to new findings. This paper was designed to demonstrate and clarify modifications relevant in practice.


METHODS
Our own experience in the production and application of monoclonal antibodies as well as the relevant literature are taken into consideration.


RESULTS
The development and production of murine and human monoclonal antibodies is described especially regarding the selection and the safety of clones. An update of the availability and use of monoclonal antibodies in respect to the different blood group systems is given, with special reference to new findings like the A1(B) and B(A) phenomena.


CONCLUSIONS
The application of monoclonal antibodies in blood group serology has improved the detection of weak variants and the availability of these reagents in general. Further improvements in this technology will make monoclonal antibodies also available for other blood group systems.
Sonneborn
H H
HH
Biotest AG, Forschungsabteilung, Dreieich.
Dahr
W
W
Helmbold
W
W
Kasulke
D
D
D000017:ABO Blood-Group System / Q000235:genetics*; D000818:Animals; D000911:Antibodies, Monoclonal; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000235:genetics*; D010641:Phenotype
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007983:Lewis Blood Group Antigens
Monoklonale Antikörper in der Blutgruppenserologie. Teil 1: Technik der Herstellung, AB0- und Lewis-System.