title: High-resolution tandem mass spectrometry of carbonic anhydrase.
abstract: Electrospray ionization of carbonic anhydrase with ion dissociation yields a mass spectrum from which the masses of > 100 isotopic clusters are determined accurately, with the number of charges assigned directly from resolved isotopic peaks. Of these clusters, 80% correspond to fragmentation at or near the amino acid proline. The masses of combinations of two, three, and four of these clusters sum to the molecular mass with 0.1-Da accuracy, while further fragment ion dissociation provides additional sequence information. The capability of this methodology to detect sequence variations is illustrated with an isozyme having a single amino acid replacement.
authors.affiliation: Department of Chemistry, Baker Laboratory, Cornell University, Ithaca, New York 14853-1301.
authors.forename: M W
authors.initials: MW
authors.lastname: Senko
authors.forename: S C
authors.initials: SC
authors.lastname: Beu
authors.forename: F W
authors.initials: FW
authors.lastname: McLafferty
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002256:Carbonic Anhydrases / Q000737:chemistry*; D013058:Mass Spectrometry; D008970:Molecular Weight
chemical_list: D000596:Amino Acids; D002256:Carbonic Anhydrases