Fluidity of chicken ventricular plasma membranes during development in-ovo and after birth: spin labelling and fluorescence studies.
The fluidity of isolated chicken ventricular plasma membranes was investigated by ESR spectroscopy, using three doxylstearic spin labels (5-SASL, 10-SASL and 16-SASL), and by fluorescence anisotropy measurements, using DPH probe. The analysis of 2T1 splitting constant and rotational correlation frequency have shown differences, during in-ovo development, characterized by a rigidification phase with a maximum at 19 days of development, just before hatching, followed by a fluidification phase after birth. These phenomena were also improved by DPH fluorescence and dynamical analysis of the hydrophobic 16-SASL probe.
Daveloose
D
D
CRSSA, unité de Biophysique, La Tronche, France.
Vezin
H
H
Basse
F
F
Viret
J
J
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology*; D002642:Chick Embryo; D002645:Chickens / Q000033:anatomy & histology* / Q000254:growth & development; D004578:Electron Spin Resonance Spectroscopy; D005454:Fluorescence Polarization; D006358:Hot Temperature; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry; D009206:Myocardium / Q000648:ultrastructure*
D008563:Membrane Lipids