Induction of apoptotic cell death in differentiating neuroblastoma SH-SY5Y cells by colchicine.
In retinoic acid (RA)-differentiated SH-SY5Y cell cultures, colchicine could induce cell death, accompanied by the typical ladder pattern of DNA fragmentation found in apoptotic cells. This effect could be attenuated by the protein synthesis inhibitor cycloheximide. The protein kinase inhibitor H-89 efficiently reduced colchicine-induced cell death. These results suggest that the mechanism for colchicine-induced cell death may act, at least in part, through the activation of apoptosis in differentiating SH-SY5Y cells.
Nakagawa-Yagi
Y
Y
Laboratory of Neurochemistry, Research Institute of Life Science, Snow Brand Milk Products, Tochigi, Japan.
D017209:Apoptosis / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003078:Colchicine / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D009447:Neuroblastoma; D009498:Neurotoxins / Q000633:toxicity*; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007546:Isoquinolines; D009498:Neurotoxins; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D014212:Tretinoin; D007770:L-Lactate Dehydrogenase; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; D003078:Colchicine