IFN-gamma, TNF, and IL-6 production by vaccinia virus immune spleen cells. An in vitro study.
The production of cytokines by virus immune spleen cells after in vitro restimulation was investigated. Vaccinia virus-primed spleen cells from CBA/H mice were stimulated in vitro with virus-infected UV-irradiated syngeneic cells. Both CD4+ and CD8+ T cell populations proliferated after restimulation. TNF, IL-6, and IFN-gamma were detected within 12 h of restimulation with maximal levels reached by 24 h. Adherent cells were major producers of IL-6 and TNF, whereas IFN-gamma production was dependent on CD4+ T cells and adherent cells. The IFN-gamma response was Ag specific, whereas the production of TNF and IL-6 was not. Stimulation with vaccinia virus constructs encoding IFN-gamma or TNF altered the levels of cytokines produced, in that IFN-gamma expression by stimulator cells led to increased IFN-gamma production, whereas TNF expression by stimulator cells augmented both TNF and IFN-gamma production by responder cells.
Carpenter
E A
EA
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Ruby
J
J
Ramshaw
I A
IA
D000818:Animals; D000941:Antigens / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D014616:Vaccinia virus / Q000276:immunology*
D000941:Antigens; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma