Biochemical, morphological and cytochemical studies of enhanced autolysis of Saccharomyces cerevisiae. 2. Morphological and cytochemical studies.
Morphological and cytochemical observation of Saccharomyces cerevisiae undergoing of induced autolysis were done in response to various chemical inducers of autolysis (NaCl, ethanol, fresh autolyzate). Changes in the inner structure of yeast cells were monitored by transmission electron microscopy and the surface of the cell wall was observed by scanning electron microscopy during autolysis. Cytochemical characterization of autolyzed cells was performed using four synthetic substrates for determination of proteinase activities but only carboxypeptidase Y could be detected in the vacuolar membranes. The morphological studies supported the data obtained from biochemical studies and confirmed that optimized conditions of autolysis have a significant effect on the structural changes of autolyzed yeast.
Kollár
R
R
Department of Biochemical Technology, Slovak Technical University, Bratislava.
Vorísek
J
J
Sturdík
E
E
D001329:Autolysis / Q000201:enzymology / Q000473:pathology; D002268:Carboxypeptidases / Q000378:metabolism; D043402:Cathepsin A; D010450:Endopeptidases / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D006651:Histocytochemistry; D007425:Intracellular Membranes / Q000201:enzymology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure*; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride / Q000494:pharmacology; D014617:Vacuoles / Q000201:enzymology
D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D012965:Sodium Chloride; D002268:Carboxypeptidases; D010450:Endopeptidases; D043402:Cathepsin A; C499283:PRC1 protein, S cerevisiae; C022633:serine carboxypeptidase