title: Induction of aromatase in stromal vascular cells from human breast adipose tissue depends on cortisol and growth factors.
abstract: The regulation of aromatase (estrogen synthase) activity of cultured stromal vascular cells from human breast adipose tissue by cortisol, db-cAMP and growth factors was studied in a serum-free culture system. While PDGF-BB alone inhibited the effect of db-cAMP on aromatase induction, it stimulated aromatase activity in the presence of cortisol with or without db-cAMP. In the presence of 1 microM insulin consistently higher aromatase activities were found as compared to 1 nM insulin. In contrast to PDGF-BB, bFGF led to an increase of aromatase activity only in the presence of both cortisol and db-cAMP.
authors.affiliation: Institute of Biochemistry, Genetics and Microbiology, University of Regensburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
authors.forename: G
authors.initials: G
authors.lastname: Löffler
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology*; D000328:Adult; D001141:Aromatase / Q000096:biosynthesis*; D000077214:Becaplermin; D001940:Breast / Q000166:cytology / Q000201:enzymology*; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004790:Enzyme Induction; D016222:Fibroblast Growth Factor 2 / Q000502:physiology*; D006801:Humans; D006854:Hydrocortisone / Q000502:physiology*; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D016895:Culture Media, Serum-Free; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D000077214:Becaplermin; D003994:Bucladesine; D001141:Aromatase; D006854:Hydrocortisone