Normal oxidative activity and chemotaxis of circulating neutrophils in patients treated with photopheresis.
In photopheresis peripheral leukocytes are exposed to photoactivated 8-methoxypsoralen in an extra-corporeal flow system. The present study was performed to characterize the effect of photopheresis upon the function of peripheral circulating neutrophils. Superoxide generating capacity and chemotactic ability of peripheral circulating neutrophils were tested in seven patients immediately before and after treatment with photopheresis. Superoxide generation stimulated by phorbol-12-myristate-13-acetate and N-formyl-methionyl-leucyl-phenylalanine (fMLP) and fMLP-induced chemotaxis were found not to be influenced by photopheresis. We conclude that circulating neutrophils obtained from patients treated with photopheresis possess normal superoxide-generating capacity and chemotactic ability.
Obel
N
N
Department of Infectious Diseases, Marselisborg Hospital, University of Aarhus, Denmark.
Storgaard
M
M
Hansen
B
B
Zachariae
H
H
D000328:Adult; D000368:Aged; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008730:Methoxsalen / Q000009:adverse effects; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010084:Oxidation-Reduction; D017893:Photopheresis / Q000009:adverse effects*; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013755:Tetradecanoylphorbol Acetate; D008730:Methoxsalen