Localization of disulfide bridges and free sulfhydryl groups in human eosinophil granule major basic protein.
Eosinophil granule major basic protein (117 residues) is known to contain free sulfhydryl groups. Here we have located in the amino acid sequence the half-cystine residues present as cysteine, and identified those engaged in disulfide bridges. Of the 9 half-cystine residues, 5 are unpaired cysteines (Cys2, Cys23, Cys42, Cys64, and Cys96), while 4 form disulfides (Cys20-Cys115, and Cys92-Cys107).
Oxvig
C
C
Department of Molecular Biology, University of Aarhus, Denmark.
Gleich
G J
GJ
Sottrup-Jensen
L
L
D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000032:analysis; D003553:Cystine / Q000032:analysis; D004220:Disulfides / Q000032:analysis*; D047091:Eosinophil Granule Proteins; D006801:Humans; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D012260:Ribonucleases; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D001798:Blood Proteins; D004220:Disulfides; D047091:Eosinophil Granule Proteins; D013438:Sulfhydryl Compounds; D003553:Cystine; D012260:Ribonucleases; D003545:Cysteine