title: Identification of the bphA4 gene encoding ferredoxin reductase involved in biphenyl and polychlorinated biphenyl degradation in Pseudomonas sp. strain KKS102.
abstract: The nucleotide sequence of the downstream region of the bph operon from Pseudomonas sp. strain KKS102 was determined. Two open reading frames (ORF1 and ORF2) were found in this region, and the deduced amino acid sequence of ORF2 showed homology with the sequences of four ferredoxin reductases of dioxygenase systems. When this region was inserted just upstream of the bph operon, which does not contain a gene encoding ferredoxin reductase, biphenyl dioxygenase activity was detected. The 24- and 44-kDa polypeptides predicted from the two open reading frames were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Crude extract which contained the products of ORF2 and bphA1A2A3 showed cytochrome c reduction activity. These data clearly suggest that ORF2 encodes ferredoxin reductase. The deduced amino acid sequence of ORF1 does not show significant homology with the sequences of any other proteins in the SWISS-PROT data bank, and the function of ORF1 is unknown.
authors.affiliation: Department of Agricultural Chemistry, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Nagata
authors.forename: M
authors.initials: M
authors.lastname: Hinata
authors.forename: K
authors.initials: K
authors.lastname: Kimbara
authors.forename: M
authors.initials: M
authors.lastname: Fukuda
authors.forename: K
authors.initials: K
authors.lastname: Yano
authors.forename: M
authors.initials: M
authors.lastname: Takagi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001713:Biphenyl Compounds / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005287:Ferredoxin-NADP Reductase / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics* / Q000502:physiology; D007506:Iron-Sulfur Proteins; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics
chemical_list: D001713:Biphenyl Compounds; D007506:Iron-Sulfur Proteins; D011078:Polychlorinated Biphenyls; D010105:Oxygenases; C074161:biphenyl-2,3-dioxygenase; D005287:Ferredoxin-NADP Reductase