The prosequence of Rhizopus niveus aspartic proteinase-I supports correct folding and secretion of its mature part in Saccharomyces cerevisiae.
Extracellular Rhizopus niveus aspartic proteinase-I (RNAP-I) was secreted effectively by Saccharomyces cerevisiae when RNAP-I with its preprosequence was synthesized in this organism (Horiuchi, H., Ashikari, T., Amachi, T., Yoshizumi, H., Takagi, M., and Yano, K. (1990) Agric. Biol. Chem. 54, 1771-1779). Certain deletions (delta pro, delta 1, delta 2), and amino acid substitutions (M1) in the prosequence blocked secretion of RNAP-I, although the protease protection assay revealed that even delta pro could be translocated across the membrane of the endoplasmic reticulum. When delta pro or M1 was synthesized simultaneously with the wild-type preprosequence in S. cerevisiae, secretion of RNAP-I was recovered. Therefore, the physical linkage of the prosequence to the mature region is not a prerequisite for secretion of active RNAP-I. Purified RNAP-I with the prosequence once denatured in 6 M guanidine HCl could be renatured and activated to have its enzymatic activity by removing guanidine HCl in vitro, but RNAP-I without the prosequence could not. Furthermore, the wild-type prosequence helped the recovery of the activity of the denatured RNAP-I in trans, but the prosequences of M1 with which secretion of RNAP-I was not observed in vivo, did not. From these results we concluded that the prosequence of RNAP-I supports correct folding of RNAP-I in the endoplasmic reticulum lumen and its subsequent secretion in S. cerevisiae. The functional role of the prosequence of an aspartic proteinase was elucidated.
Fukuda
R
R
Department of Agricultural Chemistry, University of Tokyo, Japan.
Horiuchi
H
H
Ohta
A
A
Takagi
M
M
D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D004792:Enzyme Precursors / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology*
D004792:Enzyme Precursors; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D016282:Aspartic Acid Endopeptidases; C100354:aspartic proteinase-I