title: Effect of thimerosal on cytosolic calcium and phosphatidylserine synthesis in Jurkat T cells.
abstract: 1. We investigated the effect of the thiol reagent, thimerosal on calcium movements in the Jurkat T cell line. 2. Thimerosal induced a rise in cytosolic Ca2+ concentration due both to a release of Ca2+ from intracellular stores and a Ca2+ influx. 3. Thimerosal, released Ca2+ from the same intracellular stores than CD3 mAb and ionomycin. 4. Emptying the Ca2+ intracellular stores was accompanied by a marked decrease of phosphatidylserine synthesis indicating that phosphatidylserine synthesis occurs within or close to the endoplasmic reticulum Ca(2+)-stores as previously described in CD3-, ionomycin- or Ca(2+)-ATPase inhibitor-treated lymphocytes.
authors.affiliation: INSERM U343, Faculté de Médecine, Nice, France.
authors.forename: C
authors.initials: C
authors.lastname: Pelassy
authors.forename: J P
authors.initials: JP
authors.lastname: Breittmayer
authors.forename: M
authors.initials: M
authors.lastname: Ticchioni
authors.forename: C
authors.initials: C
authors.lastname: Aussel
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003600:Cytosol / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D010718:Phosphatidylserines / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013849:Thimerosal / Q000494:pharmacology*
chemical_list: D010718:Phosphatidylserines; D013849:Thimerosal; D002118:Calcium