title: Isothermal titration calorimetric study of the association of hen egg lysozyme and the anti-lysozyme antibody HyHEL-5.
abstract: The thermodynamics of association of hen egg lysozyme and the antibody HyHEL-5 was characterized by isothermal titration calorimetry. The structure of this complex has been determined to 2.8-A resolution by Sheriff et al. [Sheriff, S., Silverton, E. W., Padlan, E. A., Cohen, G. H., Smith-Gill, S. J., Finzel, B. C., & Davies, D. R. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 8075-8079]. The calorimetric enthalpy of association is negative and declines linearly with temperature in the range 10-37 degrees C (delta Cp = -340 +/- 40 cal mol-1 K-1). Entropic contributions calculated using previously determined values of the affinity of association are negative (unfavorable) in this temperature range. This result is consistent with the loss of mobility upon association of the unusually mobile segments of HEL which form the HyHEL-5 epitope. As the affinity of association is approximately constant in this temperature range, an enthalpy-entropy compensation effect is implied. The hydrophobic and vibrational contributions to delta S and delta Cp are estimated using the method of Sturtevant [Sturtevant, J. M. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 2236-2240]. The experimental value of delta Cp is in rather close agreement with the delta Cp estimated from the polar and nonpolar surface areas buried upon association.
authors.affiliation: Department of Chemical Engineering, University of Houston, Texas 77204-4792.
authors.forename: K A
authors.initials: KA
authors.lastname: Hibbits
authors.forename: D S
authors.initials: DS
authors.lastname: Gill
authors.forename: R C
authors.initials: RC
authors.lastname: Willson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism*; D001666:Binding Sites, Antibody; D002151:Calorimetry; D002460:Cell Line; D002645:Chickens; D006825:Hybridomas; D009113:Muramidase / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D009113:Muramidase