title: Intracellular calcium modulates basolateral K(+)-permeability in frog skin epithelium.
abstract: Cytosolic calcium ([Ca2+]i) has been suggested as a key modulator in the regulation of active sodium transport across electrically "tight" (high resistance) epithelia. In this study we investigated the effects of calcium on cellular electrophysiological parameters in a classical model tissue, the frog skin. [Ca2+]i was measured with fura-2 in an epifluorescence microscope setup. An inhibition of basolateral potassium permeability was observed when cytosolic calcium was increased. This inhibition was reversible upon removal of calcium from the serosal solution.
authors.affiliation: Laboratory of Biological Chemistry A, August Krogh Institute, University of Copenhagen, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Brodin
authors.forename: K A
authors.initials: KA
authors.lastname: Rytved
authors.forename: R
authors.initials: R
authors.lastname: Nielsen
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007424:Intracellular Fluid / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects; D008564:Membrane Potentials; D010539:Permeability / Q000187:drug effects; D011188:Potassium / Q000378:metabolism*; D011896:Rana temporaria; D012867:Skin / Q000187:drug effects / Q000378:metabolism*
chemical_list: D011188:Potassium; D002118:Calcium; D007213:Indomethacin