Synthetic cDNA encoding the rat AT1a receptor: a useful tool for structure-function relationship analysis.
To carry out systematic structure-function studies of the rat angiotensin II receptors by site directed mutagenesis, or production of chimeric receptors, we have produced a synthetic cDNA coding for the AT1a receptor. The synthetic cDNA is 1101 base pairs long, and contains 49 unique restriction sites that are on the average 23 base pairs apart, allowing replacement of specific restriction fragments by synthetic counterparts containing the desired modified sequence. The total cDNA was assembled in the expression vector pECE. After stable expression in Chinese Hamster Ovary cells, the protein encoded by this synthetic cDNA presents a pharmacological profile and a signal transduction mechanism indistinguishable from the wild type rat AT1a receptor.
Conchon
S
S
Institut National de la Santé et de la Recherche Médicale Unité 36-Collège de France, Paris.
Monnot
C
C
Sirieix
M E
ME
Bihoreau
C
C
Corvol
P
P
Clauser
E
E
D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D000805:Angiotensin III / Q000494:pharmacology; D057911:Angiotensin Receptor Antagonists; D000818:Animals; D001483:Base Sequence; D001713:Biphenyl Compounds / Q000494:pharmacology; D016466:CHO Cells; D003062:Codon / Q000378:metabolism; D006224:Cricetinae; D018076:DNA, Complementary / Q000138:chemical synthesis* / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology; D007700:Kinetics; D019808:Losartan; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009842:Oligopeptides / Q000494:pharmacology; D016366:Open Reading Frames; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D011945:Receptors, Angiotensin / Q000096:biosynthesis* / Q000187:drug effects / Q000378:metabolism; D015183:Restriction Mapping; D012504:Saralasin / Q000378:metabolism / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013329:Structure-Activity Relationship; D013777:Tetrazoles / Q000494:pharmacology; D014162:Transfection
D057911:Angiotensin Receptor Antagonists; D001713:Biphenyl Compounds; D003062:Codon; D018076:DNA, Complementary; D007093:Imidazoles; D009838:Oligodeoxyribonucleotides; D009842:Oligopeptides; D011725:Pyridines; D011945:Receptors, Angiotensin; D013777:Tetrazoles; D000804:Angiotensin II; C066253:PD 123177; D000805:Angiotensin III; C060894:CGP 42112A; D012504:Saralasin; D019808:Losartan