[Study of the process of chromatin decondensation using low density agar gel electrophoresis].
A method was proposed for analysis of conformational mobility of supranucleosomal chromatin organization at different ionic conditions with the help of electrophoresis in low-density agarose gels. This simple and highly reproducible method yields the results which are in good agreement with the data of other traditional approaches. This method offers an alternative to high-speed ultracentrifugation for chromatin condensation studies.
Kraevskiĭ
V A
VA
Panin
V M
VM
Razin
S V
SV
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D008099:Liver / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism; D009994:Osmolar Concentration; D051381:Rats; D014461:Ultracentrifugation
D002843:Chromatin; D009707:Nucleosomes
Izuchenie protsessa dekondensatsii khromatina s pomoshch'iu élektroforeza v geliakh agarozy nizkoĭ plotnosti.