title: Epidermal Langerhans cells, HIV-1 infection and psoriasis.
abstract: Langerhans cells (LCs) subserve an important antigen-presenting function in the skin immune system. They bear CD4 receptors, which make them potential targets for infection with the human immunodeficiency virus (HIV-1). The observation of reduced numbers of LCs in the skin of patients with the acquired immunodeficiency syndrome (AIDS), and the association of severe psoriasis with HIV-1 infection, raise interesting questions regarding the role of LCs in the skin of HIV-1-positive psoriatic patients. In this study, LCs were quantified in the lesional and non-lesional skin of seven HIV-1-positive psoriatic patients, and the results were compared with age-, sex- and site-matched HIV-1-negative psoriatic patients. The number of LCs was determined by staining skin sections with S-100 polyclonal antibody, using the three-step avidin-biotin immunoperoxidase method. The S-100-positive cells above the basal layer were quantified in two ways: cells/mm2 of epidermal area, and cells/mm of length of basement membrane. HIV-1-positive psoriatic patients showed a reduction in the number of epidermal LCs compared with HIV-1-negative psoriatic patients using both methods of quantification, in both lesional and non-lesional skin (P < 0.05, Mann-Whitney test). In addition, a reduction in the number of LCs in lesional compared with non-lesional skin was observed in both HIV-1-positive and -negative patients when LCs were quantified per mm2 of epidermal area (P < 0.05, Wilcoxon test).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Skin and Therapeutics Research Laboratory, Chelsea and Westminster Hospital, London, U.K.
authors.forename: V
authors.initials: V
authors.lastname: Zemelman
authors.forename: F
authors.initials: F
authors.lastname: Van Neer
authors.forename: N
authors.initials: N
authors.lastname: Roberts
authors.forename: P
authors.initials: P
authors.lastname: Patel
authors.forename: J
authors.initials: J
authors.lastname: Langtry
authors.forename: R C
authors.initials: RC
authors.lastname: Staughton
mesh_terms: D000328:Adult; D002452:Cell Count; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000150:complications / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007801:Langerhans Cells / Q000473:pathology*; D008297:Male; D011565:Psoriasis / Q000150:complications / Q000473:pathology*; D012867:Skin / Q000473:pathology