Dose dependency of granulocyte-macrophage colony stimulating factor for improving survival following burn wound infection.
Infections remain a serious problem following injury. Immune modulation offers an additional strategy for the treatment of infections. We evaluated the ability of a multilineage hematopoietic growth factor, granulocyte-macrophage colony-stimulating factor (GM-CSF), to improve survival following burn injury with a superimposed burn wound infection. Groups of 12 BDF1 mice received a 15% total body surface area (TBSA) thermal injury by immersion in 100 degrees C water; 6 x 10(3) Pseudomonas was then applied to the burn wound. The GM-CSF was injected subcutaneously B.I.D. for 7 days. Mice receiving the 10-ng dose of GM-CSF had significantly improved survival compared with the controls; other doses had no significant effect on survival. Clinical trials to assess the ability of GM-CSF to reduce infectious complications following burn injury are underway and these data suggest selecting a specific dose may be critical in achieving maximal benefit.
O'Reilly
M
M
Department of Anesthesia, University of Vermont College of Medicine, Burlington.
Silver
G M
GM
Gamelli
R L
RL
Davis
J H
JH
Hebert
J C
JC
D000818:Animals; D002056:Burns / Q000150:complications / Q000188:drug therapy*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage / Q000627:therapeutic use*; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016019:Survival Analysis; D014946:Wound Infection / Q000188:drug therapy* / Q000209:etiology
D016178:Granulocyte-Macrophage Colony-Stimulating Factor