A new medium for rapid slide culture of tubercle bacilli.
Human blood medium (HBM) and egg enriched sheep blood medium (SBM) were evaluated to see their efficacy for primary culture of tubercle bacilli. SBM is found to be equally effective compared to HBM. Both types of media were associated with a low rate of contamination (2.75%). Availability of culture results after 7 days compared to 6 weeks on Lowenstein-Jensen media is a distinct advantage of slide culture method with a possible use in primary isolation and in evaluation of drug sensitivity pattern of Mycobacterium tuberculosis.
Purohit
S D
SD
Department of Chest Diseases & Tuberculosis, Sawai Man Singh Medical College, Jaipur, Rajasthan.
Gupta
M L
ML
Chauhan
A
A
Nanavati
V
V
D000818:Animals; D001431:Bacteriological Techniques; D003470:Culture Media; D005240:Feasibility Studies; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012756:Sheep; D013183:Sputum / Q000382:microbiology; D013997:Time Factors; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
D003470:Culture Media