Homogeneity of the major outer membrane protein gene of feline Chlamydia psittaci.
Thirteen feline Chlamydia psittaci strains isolated in various countries over a 50-year period were examined by restriction mapping of the major outer membrane protein (MOMP) gene obtained after DNA amplification by the polymerase chain reaction and compared with avian, ovine and guinea pig inclusion conjunctivitis (GPIC) C psittaci strains. All the feline isolates produced a unique AluI pattern distinct from the other C psittaci strains and were characterised by a typical fragment doublet of 185/180 base pairs. Such a homogeneity argues for their genetic relatedness and suggests their clonal origin. These data demonstrate the usefulness of MOMP-gene restriction mapping in C psittaci typing.
Sayada
C
C
Laboratoire de Biochimie Génétique, INSERM U120, Hôpital Robert Debré, Paris, France.
Andersen
A
A
Rodriguez
P
P
Eb
F
F
Milon
A
A
Elion
J
J
Denamur
E
E
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001717:Birds; D002415:Cats / Q000382:microbiology*; D002691:Chlamydophila psittaci / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D006168:Guinea Pigs; D006801:Humans; D012756:Sheep
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial