Cellular distribution of human leucocyte adhesion molecule ICAM-3.
OBJECTIVE
To describe the distribution of the recently cloned human leucocyte adhesion molecule ICAM-3 in normal and neoplastic tissues and cell lines.


METHODS
A panel of four monoclonal antibodies to ICAM-3 were used to stain cell lines and sections of human lymphoid tissues using the alkaline phosphatase-anti-alkaline phosphatase immunocytochemical method (APAAP).


RESULTS
In peripheral blood ICAM-3 was detected on monocytes, granulocytes, and most lymphocytes. In sections of human lymphoid tissue the antigen was also found on most lymphocytes, but many of the proliferating B cells found in the germinal centres of secondary lymphoid follicles were ICAM-3 negative. ICAM-3 was also found on neoplastic white cells (in chronic lymphocytic leukaemia, hairy cell leukaemia, acute and chronic myeloid leukaemia, and multiple myeloma) with the exception of Reed-Sternberg cells in Hodgkin's disease, many of which were negative. ICAM-3 was consistently absent from cells and tissues of non-haemopoietic origin. Endothelium (which expresses ICAM-1) was negative for ICAM-3, with the exception of vessels in some neoplastic lymphoid samples which showed variable staining for ICAM-3.


CONCLUSIONS
These findings suggest that ICAM-3 is essentially restricted to the haemopoietic system and is reciprocal in its expression to ICAM-1, in that it is present on resting cells and its level falls as a result of cell activation.
Cordell
J L
JL
Nuffield Department of Pathology, John Radcliffe Hospital, Oxford.
Pulford
K
K
Turley
H
H
Jones
M
M
Micklem
K
K
Doussis
I A
IA
Tyler
X
X
Mayne
K
K
Gatter
K C
KC
Mason
D Y
DY
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000097:blood; D006098:Granulocytes / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007938:Leukemia / Q000378:metabolism; D008214:Lymphocytes / Q000737:chemistry; D008223:Lymphoma / Q000378:metabolism; D009000:Monocytes / Q000737:chemistry; D009363:Neoplasm Proteins / Q000032:analysis; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; C483616:ICAM3 protein, human; D009363:Neoplasm Proteins