Occurrence and expression of cspA, a cold shock gene, in Antarctic psychrotrophic bacteria.
The homologue of cold shock gene cspA of Escherichia coli was detected in various isolates of Antarctic psychrotrophs representing both Gram-positive and Gram-negative bacteria. The Northern hybridization study indicated that the transcript size of cspA in the psychrotrophic Gram-positive bacterium Arthrobacter protophormiae and Gram-negative Pseudomonas fluorescens was similar to that of E. coli and that the cspA homologues in these two psychrotrophs were expressed constitutively at a low level both at 4 degrees C and 22 degrees C. In P. fluorescens, the expression of cspA mRNA was inducible after shift of temperature from 22 to 4 degrees C and the maximum level of induction occurred after 1 h which correlated with the time-lag required for growth of the culture after temperature shift.
Ray
M K
MK
Centre for Cellular and Molecular Biology, Hyderabad, India.
Sitaramamma
T
T
Ghandhi
S
S
Shivaji
S
S
D000864:Antarctic Regions; D001426:Bacterial Proteins / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D003080:Cold Temperature; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics*; D006094:Gram-Positive Bacteria / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C062260:cold shock protein CS7.4, Bacteria