Radioimmunoassay of conjugated 1 beta-hydroxycholic acid in dried blood spots for diagnosis of congenital biliary atresia.
Radioimmunological measurement of conjugated 1 beta-hydroxycholic acid in dried blood spots obtained from newborns at 4-5 d after birth has been carried out to investigate a diagnostic utility for congenital biliary atresia. The method allowed the determination of this bile acid, with a detection limit of 50 pg in one disc (3 mm i.d.), and showed good reproducibility with inter- and intra-assay coefficients with variations of 3.5-7.7% and 6.3-8.8%. With the analysis, a relatively high concentration of this substance was observed in the patients, as compared to normal subjects, averaging 1.63 ng/disc in the former and 0.97 ng/disc in the latter.
Ikegawa
S
S
Faculty of Pharmaceutical Sciences, Higashi-Nippon-Gakuen University, Hokkaido, Japan.
Murai
T
T
Matsui
A
A
Tohma
M
M
D001647:Bile Acids and Salts / Q000097:blood*; D001656:Biliary Atresia / Q000097:blood / Q000175:diagnosis*; D002793:Cholic Acids / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007231:Infant, Newborn; D007564:Japan; D011863:Radioimmunoassay
D001647:Bile Acids and Salts; D002793:Cholic Acids; C035924:1,3,7,12-tetrahydroxycholanoic acid