Different expression of beta-N-acetylhexosaminidase in mouse tissues.
The expression of genes encoding for the alpha and beta-subunits of the lysosomal enzyme beta-N-acetylhexosaminidase was investigated in different mouse tissues. It was found, using fluorogenic substrates, that the amounts of alpha and beta subunits were not the same in different tissues: alpha-subunit was more abundant in the brain, beta-subunit in epididymis and brain. The different isoenzyme patterns and specific activities in mouse tissues are due to the differences in the amount of hexosaminidase subunits. The mRNA, evaluated by Northern blotting analyses, revealed a greater expression of alpha-subunit in the testis and of beta-subunit in the brain and epididymis. The results indicate, therefore, that gene expression and the amount of subunits are in good relationship for beta-subunit, whereas there is no correlation for alpha-subunit.
Della Fazia
M A
MA
Dipartimento di Biologia Cellulare e Molecolare, Università degli Studi di Perugia, Italy.
Beccari
T
T
Servillo
G
G
Viola-Magni
M P
MP
Orlacchio
A
A
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D004822:Epididymis / Q000201:enzymology; D015870:Gene Expression; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000096:biosynthesis; D013154:Spleen / Q000201:enzymology; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology; D001619:beta-N-Acetylhexosaminidases / Q000096:biosynthesis* / Q000378:metabolism
D046911:Macromolecular Substances; D012333:RNA, Messenger; D001619:beta-N-Acetylhexosaminidases