Infusion of EPA and DHA lipid emulsions: effects on heart lipids and tolerance to ischaemia-reperfusion in the isolated rat heart.
The aim of the present study was to examine the effect of acute infusion of lipid emulsions enriched with either docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) on heart lipids, tolerance to infusion and to ischaemia-reperfusion. Isolated rat hearts were subjected to a 10 min lipid infusion period prior to 25 min of total global ischaemia and 30 min of reperfusion. Effects on physiology and metabolism were recorded during infusion and reperfusion. A more than doubled increase of DHA and a 12-fold increase of EPA in terms of relative concentration was demonstrated in the free fatty acid fraction after infusion with the respective triglyceride emulsions, without any profound change in physiology. High levels of DHA were associated with a reduced recovery of left ventricular developed pressure (LVDP) and increased release of lactate dehydrogenase (LDH) during reperfusion, while the hearts infused with the EPA-emulsion showed a recovery comparable to the control group. Heart lipid peroxidation, evaluated by release of thiobarbituric acid reactive substances (TBARS) in effluate, was about 4-fold higher in the DHA-group compared to the EPA-group during start of reperfusion and may in part explain the reduced recovery observed in these hearts. The present study demonstrates enrichment of DHA and EPA in the free fatty acid fraction after a short period of infusion. Protective effects of the emulsions were not found, instead the data indicate harmful effects of DHA during ischaemia-reperfusion. However, the presence of TBARS in this emulsion could have influenced the results.
Schjøtt
J
J
Department of Pharmacology and Toxicology, University Medical Center, University of Trondheim, Norway.
Brekke
O L
OL
Jynge
P
P
Bjerve
K S
KS
Hamazaki
T
T
D000818:Animals; D004281:Docosahexaenoic Acids / Q000032:analysis / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000032:analysis / Q000494:pharmacology*; D005217:Fat Emulsions, Intravenous; D005227:Fatty Acids / Q000032:analysis; D006321:Heart / Q000187:drug effects*; D015227:Lipid Peroxidation; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D005217:Fat Emulsions, Intravenous; D005227:Fatty Acids; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid