Topical application and uptake of vitamin E acetate by the skin and conversion to free vitamin E.
Radioactive tocopherol acetate was diluted with either (1) unlabelled tocopherol acetate or (2) Delios S (Henkel, a medium chain triglyceride prepared from fractionated coconut oil), a cosmetic base. These preparations were applied topically to a 2 cm diameter circle of skin. After 24 hours the percent of label which was still removable by swabbing the skin surface was 1.7% for (1) and 11.5% for (2). The central circles contained 2.86% of the label applied in 259 mg skin for (1) and 24.2% of the label applied in 226 mg skin for Delios S for (2). Surprisingly, combined samples of approximately one third of the side skin contained 0.7% of the label applied in 460 mg for (1) and 13.2% of the applied label in one third of the side skin in 523 mg for (2). The percent conversion to tocopherol in the skin central areas was 4.52% by HPLC and 4.13% by TLC for (1) and 5.97% for (2). In the side skin the percent conversion to tocopherol was 5.0% for (1) and 6.01% for (2).
Trevithick
J R
JR
Department of Biochemistry, Faculty of Medicine, University of Western Ontario, London, Canada.
Mitton
K P
KP
D000287:Administration, Topical; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D008297:Male; D051379:Mice; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D014810:Vitamin E; D024502:alpha-Tocopherol; D024505:Tocopherols