Mineralization of monofluorobenzoate by a diculture under sulfate-reducing conditions.
A mesophilic, dehalogenating, sulfate-reducing diculture was isolated from an anaerobic lake sediment. One strain of the diculture is proposed to be an endospore-forming Desulfotomaculum species, the second strain was a vibrioid, motile and non-sporeforming species which is tentatively assigned to the genus Desulfovibrio. The diculture was able to mineralize 4- and 2-fluorobenzoate both isomers being incompletely oxidized with the release of acetate, which was subsequently used by both sulfate-reducing strains. Other electron donors used for growth included benzoate, 3- and 4-hydroxybenzoate, protocatechuate, catechol, phenol, 2,5-dimethoxyphenol, fatty acids up to C8, malate and pyruvate. The culture obtained from a freshwater habitat grew optimally at NaCl concentrations of 0.3-0.5 g l-1, 33-37 degrees C, and pH 7.4. Our experiments showed that certain fluorinated aromatic hydrocarbons could serve as sole sources of carbon and energy for sulfate-reducing bacteria.
Drzyzga
O
O
Fachbereich Biologie, AG Mikrobiologie, Universität Oldenburg, FRG.
Jannsen
S
S
Blotevogel
K H
KH
D001565:Benzoates / Q000378:metabolism*; D003901:Desulfovibrio / Q000378:metabolism; D005618:Fresh Water; D062385:Hydroxybenzoates / Q000378:metabolism; D010084:Oxidation-Reduction; D010636:Phenols / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D016999:Sulfur-Reducing Bacteria / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D014871:Water Microbiology
D001565:Benzoates; D062385:Hydroxybenzoates; D010636:Phenols; D013431:Sulfates; C022894:2-fluorobenzoic acid; C022893:4-fluorobenzoic acid