Automated assay for GV104326, a novel tribactam antibiotic, in human plasma by high-performance liquid chromatography and solid-phase extraction.
A highly automated, rapid, robust and specific plasma assay for GV104326, a novel tribactam antibiotic, has been developed to monitor human volunteer trials. The method involves automated solid-phase extraction with a strong anion-exchange phase and HPLC on a reversed-phase column with ultraviolet detection. The calibration range for the assay is 0.05-2 micrograms/ml. The assay is linear over this range and is specific with respect to endogenous interference and likely metabolites of GV104326. Both intra- and inter-assay variability were < 8% and intra- and inter-day bias < 10%.
Barnaby
R J
RJ
Research Laboratories, Glaxo S.p.A., Verona, Italy.
Iavarone
L
L
D000837:Anion Exchange Resins; D000900:Anti-Bacterial Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D006801:Humans; D007769:Lactams; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000837:Anion Exchange Resins; D000900:Anti-Bacterial Agents; C086671:GV 104326; D007769:Lactams