Studies on azole-induced cell death in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae strain XL16-5B exhibited a fungicidal response to treatment with ketoconazole. Cell death became apparent during prolonged treatment over 72 h following an initial period over 24 h where viable cells were found and limited cell division occurred. Sterol analysis showed some differences between XL16-5B and the strain XY729-5a, which had a fungistatic response to ketoconazole. In particular, the level of ergosterol was higher in XL16-5B and remained high during treatment.
Kelly
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Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, Sheffield University, UK.
Kenna
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Arnoldi
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Kelly
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D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004875:Ergosterol / Q000032:analysis; D007654:Ketoconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D013045:Species Specificity; D013261:Sterols / Q000032:analysis
D013261:Sterols; D003577:Cytochrome P-450 Enzyme System; D007654:Ketoconazole; D004875:Ergosterol