Studies on the immunogenicity of a recombinant ookinete surface antigen Pbs21 from Plasmodium berghei expressed in Escherichia coli.
Plasmodium berghei ookinete surface antigen (Pbs21), was produced as a fusion product with maltose binding protein (MBP) in Escherichia coli and used to induce transmission-blocking immunity in mice. Specificity of induced antibody was confirmed by Western blotting with native ookinete Pbs21, and by the indirect immunofluorescent antibody test on ookinete bloodfilms. Immunized mice were infected with P. berghei and transmission to Anopheles stephensi mosquitoes determined by both the intensity and prevalence of oocyst infections. Compared with a control group immunized with MBP alone the maximum blockade of oocyst intensity was 66% in the mice immunized with recombinant MBP-Pbs21. Over nine experiments blockade averaged only 33%. By comparison with native Pbs21 protein, which usually induces > or = 90% blockade, our data suggests the recombinant protein produced in this bacterial system is a less effective immunogen despite expressing epitopes recognized by known transmission-blocking monoclonal antibodies.
Matsuoka
H
H
Department of Pure and Applied Biology, Imperial College of Science, Technology and Medicine, London, UK.
Paton
M G
MG
Barker
G C
GC
Alejo Blanco
A R
AR
Sinden
R E
RE
D000818:Animals; D000852:Anopheles / Q000469:parasitology; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000097:blood; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D005455:Fluorescent Antibody Technique; D007114:Immunization; D008288:Malaria / Q000517:prevention & control*; D010962:Plasmodium berghei / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000954:Antigens, Surface; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; C084246:ookinete surface antigen Pbs21, Plasmodium