Functional comparison between HTLV-I envelopes originating from TSP/HAM or ATL cell lines.
The human T-cell leukemia type I (HTLV-I) virus is associated with two different diseases, adult T-cell leukemia (ATL) and tropical spastic paraparesis/HTLV-I-associated myelopathy (TSP/HAM). We have compared the viral envelopes originating from TSP/HAM and ATL patients, using the capacity of infected cells to form syncytia with receptor-expressing cells. We show that like the ATL cell lines, the TSP/HAM ones can form syncytia with a large panel of human target cells, including a variety of hematopoietic cell lines, as well as cell lines of neuroectodermal origin. None of the target cell lines tested was able to discriminate between TSP/HAM- and ATL-infected cell lines. When infected cells of TSP/HAM origin are cocultivated with cells of ATL origins, syncytia are never observed. This interference phenomenon suggests that the viruses expressed by the different cell lines utilize the same receptor.
Pique
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CNRS URA 1156, Institut Gustave Roussy, Villejuif, France.
Saal
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F
Périès
J
J
Pham
D
D
Tursz
T
T
Dokhélar
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MC
D015153:Blotting, Western; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D015726:Giant Cells; D006412:Hematopoietic Stem Cells / Q000166:cytology; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D015458:Leukemia, T-Cell / Q000382:microbiology* / Q000473:pathology; D015493:Paraparesis, Tropical Spastic / Q000382:microbiology* / Q000473:pathology; D011991:Receptors, Virus / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology / Q000382:microbiology*; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000502:physiology*
D011991:Receptors, Virus; D014759:Viral Envelope Proteins