Cloning and expression of the bovine sodium- and chloride-dependent noradrenaline transporter.
A cDNA encoding a functional bovine, tricyclic antidepressant-sensitive noradrenaline transporter has been identified by screening a lambda gt11 cDNA library of the bovine adrenal medulla using the cDNA of the human noradrenaline transporter [1991, Nature 350, 350-354]. The sequence predicts a protein of 615 amino acids (M(r) 68,900). The bovine transporter shares 93% amino acid identity with the human sequence, but displays two more consensus sites for phosphorylation by protein kinase C. Transient expression of the transporter in COS-7 cells resulted in a sodium- and chloride-dependent uptake of noradrenaline with a pharmacology typical for a neuronal noradrenaline transporter.
Lingen
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Institut für Pharmakologie und Toxikologie, Universität Bonn, Germany.
Brüss
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Bönisch
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009638:Norepinephrine / Q000378:metabolism; D050484:Norepinephrine Plasma Membrane Transport Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D027981:Symporters
D002352:Carrier Proteins; D050484:Norepinephrine Plasma Membrane Transport Proteins; D027981:Symporters; D009638:Norepinephrine