title: [The bovine zona pellucida: differences in macromolecular composition between oocytes, pre-treated with A-23187 or not, and embryos].
abstract: The SDS-PAGE method was used to determine the composition of isolated bovine zona pellucida (ZP). This egg extracellular coat appears to be characterized by 3 major glycoproteins (ZP1, ZP2, ZP3), with apparent molecular weight (MW) of 80-70 kD, 66-63 kD and 60 kD, respectively, as revealed by 1-dimensional SDS-PAGE. After 2-dimensional SDS-PAGE, the zona pellucida electrophoretic pattern indicates a fourth glycoprotein, thus called ZP4. SDS-PAGE analysis of ZP isolated from oocytes and embryos after transit through the genital tract of A23187 pretreated oocytes allowed the description of modifications in glycoproteinic composition.
authors.affiliation: CHU-HME, laboratoire de Biologie de la reproduction, Nantes, France.
authors.forename: S
authors.initials: S
authors.lastname: Bercegeay
authors.forename: F
authors.initials: F
authors.lastname: Allaire
authors.forename: M
authors.initials: M
authors.lastname: Jean
authors.forename: A
authors.initials: A
authors.lastname: L'Hermite
authors.forename: J F
authors.initials: JF
authors.lastname: Bruyas
authors.forename: N
authors.initials: N
authors.lastname: Renard
authors.forename: D
authors.initials: D
authors.lastname: Tainturier
authors.forename: P
authors.initials: P
authors.lastname: Barrière
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000737:chemistry*; D000001:Calcimycin / Q000494:pharmacology*; D002417:Cattle / Q000196:embryology / Q000378:metabolism*; D004527:Egg Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005326:Fetal Proteins / Q000032:analysis*; D008562:Membrane Glycoproteins / Q000032:analysis*; D008970:Molecular Weight; D009865:Oocytes / Q000737:chemistry* / Q000187:drug effects; D011956:Receptors, Cell Surface; D015044:Zona Pellucida / Q000737:chemistry* / Q000187:drug effects; D000072216:Zona Pellucida Glycoproteins
chemical_list: D004527:Egg Proteins; D005326:Fetal Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; D000001:Calcimycin
vernacular_title: La zone pellucide bovine: différences de composition macromoléculaire entre ovocytes, prétraités ou non à l'A 23187, et embryons.