title: Localization of disulfide bridges and free sulfhydryl groups in human eosinophil granule major basic protein.
abstract: Eosinophil granule major basic protein (117 residues) is known to contain free sulfhydryl groups. Here we have located in the amino acid sequence the half-cystine residues present as cysteine, and identified those engaged in disulfide bridges. Of the 9 half-cystine residues, 5 are unpaired cysteines (Cys2, Cys23, Cys42, Cys64, and Cys96), while 4 form disulfides (Cys20-Cys115, and Cys92-Cys107).
authors.affiliation: Department of Molecular Biology, University of Aarhus, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Oxvig
authors.forename: G J
authors.initials: GJ
authors.lastname: Gleich
authors.forename: L
authors.initials: L
authors.lastname: Sottrup-Jensen
mesh_terms: D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000032:analysis; D003553:Cystine / Q000032:analysis; D004220:Disulfides / Q000032:analysis*; D047091:Eosinophil Granule Proteins; D006801:Humans; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D012260:Ribonucleases; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D004220:Disulfides; D047091:Eosinophil Granule Proteins; D013438:Sulfhydryl Compounds; D003553:Cystine; D012260:Ribonucleases; D003545:Cysteine