[Application of the time-resolved immunofluorometric assay in the detection of viruses].
Two systems of time-resolved immunofluorometric assay (TR-IFMA), i.e., LKB system detection of viruses. The viruses detected were mainly adenovirus, influenza virus, parainfluenza virus, respiratory syncytial virus rotavirus, hepatitis B virus, human immunodeficiency virus, rubella virus, etc. Compared with methods of ELISA and RIA, TR-IFMA has the merits of high sensitivity, specificity and consistency with clinical diagnosis. The low detection limit of virus antigen by TR-IFMA is between 10-100 pg. The application of dual-label TR-IFMA also showed good effect. TR-IFMA can also be used in the detection of viral nucleic acids. The target region detected may reach the level of pg.
Zuo
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Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing.
D000256:Adenoviridae / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000097:blood; D004279:DNA, Viral / Q000097:blood; D015200:Fluoroimmunoassay; D015483:HIV Antibodies / Q000097:blood*; D015497:HIV-1 / Q000302:isolation & purification*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans
D000956:Antigens, Viral; D004279:DNA, Viral; D015483:HIV Antibodies