Evidence for both tyrosine kinase and G-protein-coupled pathways leading to starfish egg activation.
To investigate possible pathways leading to egg activation at fertilization, the ability of exogenously introduced tyrosine kinase and G-protein-coupled receptors to mimic events of fertilization was examined. Oocytes of the starfish Asterina miniata were injected with RNA for a chimeric receptor consisting of the extracellular domain of the beta form of the mouse platelet-derived growth factor (PDGF) receptor and the transmembrane/intracellular domain of the human fibroblast growth factor (FGF) receptor, or with RNA for the rat serotonin 1c receptor. These oocytes were cultured for 1 to 3 days and then matured with 1-methyladenine. In response to PDGF or serotonin, the injected eggs underwent responses like those at fertilization: cortical granule exocytosis, a rise in intracellular free calcium, and DNA synthesis. Some of these artificially activated eggs cleaved, and some of the PDGF-activated eggs were observed to form larvae. A PDGF/FGF receptor with a point mutation which eliminated its ability to interact with phospholipase C-gamma did not cause fertilization-like responses. Thus components of a signaling pathway involving phospholipase C-gamma, characteristic of tyrosine kinase receptors, as well as components of a pathway involving a G-protein and phospholipase C-beta, characteristic of G-protein-coupled receptors, appear to be present in starfish eggs. Either or both could function in egg activation at fertilization.
Shilling
F M
FM
Department of Physiology, University of Connecticut Health Center, Farmington 06032.
Carroll
D J
DJ
Muslin
A J
AJ
Escobedo
J A
JA
Williams
L T
LT
Jaffe
L A
LA
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005260:Female; D005306:Fertilization; D019204:GTP-Binding Proteins / Q000502:physiology*; D007814:Larva / Q000254:growth & development; D008297:Male; D010063:Ovum / Q000502:physiology*; D020794:Receptor Protein-Tyrosine Kinases / Q000502:physiology*; D017468:Receptors, Fibroblast Growth Factor / Q000502:physiology; D017479:Receptors, Platelet-Derived Growth Factor / Q000502:physiology; D011985:Receptors, Serotonin / Q000502:physiology; D013215:Starfish / Q000502:physiology*; D010738:Type C Phospholipases / Q000502:physiology
D017468:Receptors, Fibroblast Growth Factor; D011985:Receptors, Serotonin; D004247:DNA; D020794:Receptor Protein-Tyrosine Kinases; D017479:Receptors, Platelet-Derived Growth Factor; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins; D002118:Calcium