Immunohistochemical localization of transforming growth factor-alpha in choroid plexus of the rat and chicken.
Transforming growth factor-alpha (TGF-alpha) is a polypeptide which binds to epidermal growth factor-receptor (EGF-R) to carry out its function. We have observed strong TGF-alpha immunoreactivity in the developing and adult choroid plexus of the rat and chicken and glial cells of adult rats, by using a well characterized monoclonal antibody and the peroxidase method. Preabsortion of the antibody with the peptide gave negative staining. Since previous studies have shown that EGF-R is localized in several brain regions, but not in the choroid plexus, during development and adulthood, it is suggested that TGF-alpha, synthesized in the choroid plexus and transferred to the cerebrospinal fluid, has a role in brain development. TGF-alpha immunoreactivity found in glial cells, but not in neurons of adult rats, suggests that TGF-alpha in adulthood is also synthetized in the glial cell compartment.
Díaz-Ruiz
C
C
Departament de Biologia Cel.lular i Anatomia Patològica, Hospital Princeps d'Espanya, Barcelona, Spain.
Pérez-Tomás
R
R
Domingo
J
J
Ferrer
I
I
D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens; D002831:Choroid Plexus / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D016211:Transforming Growth Factor alpha / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D016211:Transforming Growth Factor alpha