Use of body fluid of adult female Ascaris suum as an antigen in the enzyme-linked immunosorbent assay (ELISA) for diagnosis of swine ascariosis.
The Enzyme-linked immunosorbent assay (ELISA) was used to detect Ascaris suum antibodies in swine sera with adult body fluid (ABF) as an antigen. The assay was standardized with respect to the antigen concentration and serum and conjugate dilutions. Cross reaction was found between the antigen and the sera from the swine infected with Metastrongylus apri. The ELISA was more sensitive than the complement fixation test. Five protein peaks were obtained from ABF by gel filtration on Sephacryle S-300. Fraction 1 was the most specific. A 105 kDa protein in the fraction reacted with swine IgG in the serum of infected animals in Western blot analysis.
Yoshihara
S
S
Kyushu Branch Laboratory, National Institute of Animal Health, Kagoshima, Japan.
Oya
T
T
Furuya
T
T
Goto
N
N
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth; D001196:Ascariasis / Q000175:diagnosis / Q000662:veterinary*; D017165:Ascaris suum / Q000276:immunology*; D015153:Blotting, Western; D001826:Body Fluids / Q000276:immunology; D002850:Chromatography, Gel; D003168:Complement Fixation Tests; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion; D008297:Male; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera