title: Simultaneous determination of felbamate and four metabolites in rat cerebrospinal fluid by high-performance liquid chromatography.
abstract: An isocratic liquid chromatographic method for direct sample injection has been developed for the quantitation of felbamate and four metabolites in rat cerebrospinal fluid. The method uses 0.050- or 0.025-ml aliquots of cerebrospinal fluid diluted with equal volumes of internal standard. Chromatography is performed on a 150 mm x 4.6 mm I.D. Spherisorb ODS2, 3-microns HPLC column eluted with a phosphate buffer-acetonitrile-methanol (820:120:60, v/v/v) mobile phase and ultraviolet absorbance detection at 210 nm. The linear quantitation ranges are: felbamate and the 2-hydroxy metabolite 0.195-200 micrograms/ml, the propionic acid metabolite 0.195-50.0 micrograms/ml, the p-hydroxy metabolite 0.781 to 50.0 micrograms/ml, and the monocarbamate metabolite 0.098-50.0 micrograms/ml.
authors.affiliation: Department of Biochemistry, Wallace Laboratories, Division of Carter-Wallace, Cranbury, NJ 08512.
authors.forename: L A
authors.initials: LA
authors.lastname: Romanyshyn
authors.forename: J K
authors.initials: JK
authors.lastname: Wichmann
authors.forename: N
authors.initials: N
authors.lastname: Kucharczyk
authors.forename: R D
authors.initials: RD
authors.lastname: Sofia
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000134:cerebrospinal fluid* / Q000493:pharmacokinetics; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D000078328:Felbamate; D048448:Phenylcarbamates; D011409:Propylene Glycols / Q000134:cerebrospinal fluid* / Q000493:pharmacokinetics; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000927:Anticonvulsants; D048448:Phenylcarbamates; D011409:Propylene Glycols; D000078328:Felbamate