title: Induction of expression of adhesion molecules on an eosinophilic cell line (EoL-1) by the supernatant of lymphocytes stimulated with specific allergen from asthmatic patients.
abstract: Recently, adhesion molecules have been considered to play an important role in inflammatory processes in bronchial asthma. To extend our understanding of the high-intensity expression of adhesion molecules (CR3, LFA-1 alpha, LFA-1 beta, ICAM-1) on hypodense eosinophils, which was observed in our previous study, we examined whether the supernatant of lymphocytes from mite-allergic asthmatic patients is involved in adhesion molecule expression using an eosinophilic cell line (EoL-1). These characteristics of adhesion molecule expression were induced by the supernatant of lymphocytes obtained from mite-allergic asthmatic patients cultured with specific allergen as well as a combination of recombinant cytokines (IL-3, GM-CSF, IL-5). Thus, we could conclude that some lymphokines produced by specific allergen in asthma might be involved in the high-intensity expression of adhesion molecules on hypodense eosinophils in asthma or allergic disorders.
authors.affiliation: Fourth Department of Internal Medicine, Kinki University School of Medicine, Osakasayama, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Chihara
authors.forename: D
authors.initials: D
authors.lastname: Kurachi
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: I
authors.initials: I
authors.lastname: Higashimoto
authors.forename: T
authors.initials: T
authors.lastname: Kakazu
authors.forename: S
authors.initials: S
authors.lastname: Nakajima
mesh_terms: D000485:Allergens / Q000009:adverse effects; D000818:Animals; D001249:Asthma / Q000097:blood* / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002460:Cell Line; D004804:Eosinophils / Q000737:chemistry*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D006801:Humans; D007377:Interleukin-3 / Q000502:physiology; D015848:Interleukin-5 / Q000502:physiology; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D008214:Lymphocytes / Q000276:immunology; D016177:Macrophage-1 Antigen / Q000502:physiology; D008925:Mites / Q000276:immunology
chemical_list: D000485:Allergens; D015815:Cell Adhesion Molecules; D007377:Interleukin-3; D015848:Interleukin-5; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor