Anticardiolipin antisera from lupus patients with seizures reduce a GABA receptor-mediated chloride current in snail neurons.
The effects of circulating anticardiolipin (ACL) antisera in lupus patients on the LP5 central neuron of snail were studied. Both GABA and glutamate increased a chloride conductance of the LP5 neuron. The ACL antisera decreased the GABA-elicited responses in a concentration dependent manner while it had no effect on glutamate-elicited responses. The ACL antisera affected neither the resting membrane current, nor the membrane conductivity of neuron. Antisera without the activity of anticardiolipin did not decrease the GABA-elicited responses. The seizure incidence of the patients with higher ACL antisera levels is also higher. It is concluded that ACL antisera inhibited the GABA ionophore receptor complex in a snail central neuron.
Liou
H H
HH
Department of Neurology, College of Medicine, National Taiwan University, Taipei, R.O.C.
Wang
C R
CR
Chou
H C
HC
Arvanov
V L
VL
Chen
R C
RC
Chang
Y C
YC
Chuang
C Y
CY
Chen
C Y
CY
Tsai
M C
MC
D000818:Animals; D017153:Antibodies, Anticardiolipin / Q000494:pharmacology*; D018118:Chloride Channels / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000150:complications / Q000276:immunology*; D008564:Membrane Potentials; D009474:Neurons / Q000378:metabolism*; D018079:Receptors, GABA / Q000378:metabolism*; D012640:Seizures / Q000150:complications*; D012908:Snails
D017153:Antibodies, Anticardiolipin; D018118:Chloride Channels; D007106:Immune Sera; D018079:Receptors, GABA