title: An increase of 63 kDa-protein present in the cell membranes of Staphylococcus aureus that bears a plasmid mediating inducible resistance to partial macrolide and streptogramin B antibiotics.
abstract: A plasmid, pEP2104 (23.9 kilobase pairs), from Staphylococcus aureus carries a gene that specifies inducible resistance to 14-membered (erythromycin, EM, and oleandomycin, OL) and 16-membered macrolide (mycinamicin I and II), but not to all of the latter, and to streptogramin type B antibiotics (partial macrolide- and streptogramin-B-antibiotic resistance: PMS-resistance) (L. Jánosi, E. Bán, Acta Microbiol. Acad. Sci. Hung., 29, 187 (1982) and Y. Nakajima et al., J. Pharmacobio-Dyn., 15, 319 (1992)). The induced cells of strain 8325(pEP2104) did not inactivate EM, OL, josamycin, rokitamycin or mikamycin B (MKM-B), and the cell-free extract of the strain did not inactivate EM or MKM-B, either. Ribosomes from the cells whose PMS-resistance was induced by EM were sensitive not only to EM or spiramycin, but also to MKM-B. A 63000-dalton protein increased to a great extent only in the cell membrane fractions of induced 8325(pEP2104), and may be involved in PMS-resistance.
authors.affiliation: Division of Microbiology, Hokkaido Institute of Pharmaceutical Sciences, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsuoka
authors.forename: L
authors.initials: L
authors.lastname: Jánosi
authors.forename: K
authors.initials: K
authors.lastname: Endou
authors.forename: S
authors.initials: S
authors.lastname: Saitoh
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Nakajima
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D018942:Macrolides; D008565:Membrane Proteins / Q000378:metabolism*; D010957:Plasmids; D012270:Ribosomes / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism*; D014769:Virginiamycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D008565:Membrane Proteins; D014769:Virginiamycin