title: Inhalation of resuspended road dust, but not ammonium nitrate, decreases the expression of the pulmonary macrophage Fc receptor.
abstract: Pulmonary macrophages (PM) play a key role in the immune defenses of the lung. When stimulated, PM express Fc receptors (FcR) that regulate the immune response. PM were assayed for FcR expression following subchronic inhalation exposure of adult Fischer 344 rats to either 90 micrograms/m3 nitrate (NH4NO3), 300 micrograms/m3 road dust, or clean air, for 4 h/day, 4 days/week, for 8 weeks. PM were lavaged from the lungs and attached to glass coverslips for 18 h. PM FcR were labelled with rat IgG conjugated with cyanine-3. For each exposure, FcR were determined with a Meridian ACAS 570 confocal cytometer by imaging the fluorescence of 50 cells. We found that the IgG binding to FcR (in arbitrary fluorescence units, FU, per cell) for PM from road dust exposed rats was less (835 +/- 39.3 FU/cell) than that for PM from both ammonium nitrate or clean air-exposed rats (1115 +/- 58.0 FU/cell and 1123 +/- 46.6 FU/cell, respectively). While acid incubation conditions in vitro (pH 5.5 for 30 min to simulate the acid environment of ammonium nitrate inhalation) resulted in a 16% decrease in IgG binding (P < 0.05), IgG binding to PM from acid aerosol exposed rats was no different than the IgG bound to PM from clean air-exposed rats. PM exposed to road dust in vivo expressed 25% fewer FcR (P < 0.05). Three-dimensional images of PM failed to show any major alterations in FcR distribution. These preliminary results indicate cellular recognition of antibody-immune complexes may be impaired by subchronic exposure to road dust, which could decrease the immune response of road dust exposed animals.
authors.affiliation: Department of Community and Environmental Medicine, University of California, Irvine 92717-1825.
authors.forename: B
authors.initials: B
authors.lastname: Ziegler
authors.forename: D K
authors.initials: DK
authors.lastname: Bhalla
authors.forename: R E
authors.initials: RE
authors.lastname: Rasmussen
authors.forename: M T
authors.initials: MT
authors.lastname: Kleinman
authors.forename: D B
authors.initials: DB
authors.lastname: Menzel
mesh_terms: D000818:Animals; D004391:Dust / Q000009:adverse effects*; D004781:Environmental Exposure / Q000009:adverse effects*; D007091:Image Processing, Computer-Assisted; D007074:Immunoglobulin G / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009566:Nitrates / Q000009:adverse effects*; D051381:Rats; D011916:Rats, Inbred F344; D011961:Receptors, Fc / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004391:Dust; D007074:Immunoglobulin G; D009566:Nitrates; D011961:Receptors, Fc; C006568:ammonium nitrate