title: Betel nut (Areca catechu) consumption and the induction of glucose intolerance in adult CD1 mice and in their F1 and F2 offspring.
abstract: Many mutagenic nitroso compounds are also diabetogenic. Betel-nut (Areca catechu) chewing populations have an increased incidence of foregut cancers related to betel-nut nitrosamines which suggests that betel consumption could be diabetogenic. Young adult CD1 mice with a low spontaneous incidence of diabetes were fed betel nut in standard feed for 2-6 days. Single point (90 min) intra-peritoneal glucose tolerance tests were used to follow glucose tolerance up to 6 months of age. Glucose intolerance was defined as over 3 SD above mean control values. Glucose intolerance was found in 3 of 51 male and 4 of 33 female adult mice which were fed the betel diet (p < 0.01). Studies on the progeny of these mice are presented separately for animals studied in Aberdeen (Group 1) and London (Group 2). In matings of Group 1 betel-fed parents glucose intolerance was found in 4 of 25 male and 1 of 22 female F1 offspring, with significant hyperglycaemia in F1 males born to hyperglycaemic but not to normoglycaemic mothers (p < 0.01). In the F2 generation 4 of 23 males and 1 of 16 females and in the F3 generation 1 of 16 males and 0 of 20 females were glucose intolerant. In the Group 2 studies where betel-fed parents were mated to normal controls glucose intolerance was found in 10 of 35 male and 10 of 33 female F1 progeny (p < 0.005), and mean islet areas were increased in offspring of betel-fed parents (p < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Cellular Mechanisms Research Group, London Hospital Medical College, UK.
authors.forename: B J
authors.initials: BJ
authors.lastname: Boucher
authors.forename: S W
authors.initials: SW
authors.lastname: Ewen
authors.forename: J M
authors.initials: JM
authors.lastname: Stowers
mesh_terms: D000818:Animals; D001114:Areca; D003433:Crosses, Genetic; D005260:Female; D018149:Glucose Intolerance / Q000209:etiology* / Q000235:genetics; D005951:Glucose Tolerance Test; D007515:Islets of Langerhans / Q000473:pathology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009602:Nitrosamines / Q000633:toxicity*; D009754:Nuts; D010182:Pancreatic Diseases / Q000209:etiology / Q000473:pathology*; D010946:Plants, Medicinal; D012016:Reference Values; D012737:Sex Factors
chemical_list: D009602:Nitrosamines