title: Visible-light and X irradiations of Chinese hamster lung cells treated with hematoporphyrin derivative.
abstract: Single cell suspensions of Chinese hamster lung cells were treated with hematoporphyrin derivative (HPD) and were exposed under aerobic conditions to visible light alone, X rays alone or light and X rays concurrently. Cytotoxicity was assayed using the colony formation ability of cells as the end point. The drug toxicity, phototoxicity, radiosensitization and photoradiosensitization of HPD were examined for a drug concentration of 1 microgram/ml and incubation time of 24 h. In these experiments, the X-ray dose was 3 Gy and the energy fluence of visible light was 0.35 kJ/m2, and both irradiations lasted for 10 min. A significant enhancement in cytotoxicity was observed when the HPD-treated cells were irradiated concurrently with light and X rays. However, no significant enhancement was observed when visible-light and X irradiations were performed sequentially with a 15-min waiting time between the two irradiations.
authors.affiliation: Yale University School of Medicine, Department of Therapeutic Radiology, New Haven, Connecticut 06510.
authors.forename: G
authors.initials: G
authors.lastname: Kavarnos
authors.forename: R
authors.initials: R
authors.lastname: Nath
authors.forename: P
authors.initials: P
authors.lastname: Bongiorni
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D017324:Hematoporphyrin Derivative / Q000494:pharmacology*; D008027:Light; D008168:Lung / Q000187:drug effects / Q000528:radiation effects; D010778:Photochemotherapy; D013535:Suspensions; D014965:X-Rays
chemical_list: D013535:Suspensions; D017324:Hematoporphyrin Derivative