Formation of nascent intercalated disks between grafted fetal cardiomyocytes and host myocardium.
Fetal cardiomyocytes isolated from transgenic mice carrying a fusion gene of the alpha-cardiac myosin heavy chain promoter with a beta-galactosidase reporter were examined for their ability to form stable intracardiac grafts. Embryonic day 15 transgenic cardiomyocytes delivered directly into the myocardium of syngeneic hosts formed stable grafts, as identified by nuclear beta-galactosidase activity. Grafted cardiomyocytes were observed as long as 2 months after implantation, the latest date assayed. Intracardiac graft formation did not induce overtly negative effects on the host myocardium and was not associated with chronic immune rejection. Electron microscopy revealed the presence of nascent intercalated disks connecting the engrafted fetal cardiomyocytes and the host myocardium. These results suggest that intracardiac grafting might provide a useful approach for myocardial repair, provided that the grafted cells can contribute to myocardial function.
Soonpaa
M H
MH
Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis 46202-4800.
Koh
G Y
GY
Klug
M G
MG
Field
L J
LJ
D000818:Animals; D001483:Base Sequence; D002450:Cell Communication; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D017690:Cell Transplantation; D004247:DNA / Q000096:biosynthesis; D017931:DNA Primers; D004562:Electrocardiography; D005318:Fetal Heart / Q000166:cytology*; D016332:Fetal Tissue Transplantation; D017629:Gap Junctions / Q000502:physiology / Q000648:ultrastructure; D005819:Genetic Markers; D006321:Heart / Q000502:physiology; D007365:Intercellular Junctions / Q000502:physiology / Q000648:ultrastructure*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009206:Myocardium / Q000166:cytology* / Q000648:ultrastructure; D001616:beta-Galactosidase / Q000032:analysis
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA; D001616:beta-Galactosidase