Alteration of the proteoglycan form of macrophage colony-stimulating factor produced by a human stromal line stimulated by tumor necrosis factor-alpha.
Immunoblot analysis of macrophage colony-stimulating factor (M-CSF) in KM 102 cell-conditioned medium showed the presence of two M-CSF molecular types, one being 85-kd M-CSF, the other a proteoglycan form (PG-M-CSF) carrying a chondroitin sulfate chain of variable length. When KM 102 cells were stimulated by TNF-alpha, they produced more M-CSF than that produced in unstimulated condition, in which PG-M-CSF had a shorter chondroitin sulfate chain. Although PG-M-CSF has binding affinity for type V collagen, the PG-M-CSF with the shorter chondroitin sulfate chain shows lower affinity. This spreads in type V collagen-containing agarose gel more easily than does PG-M-CSF with a longer chondroitin sulfate chain.
Ohtsuki
T
T
Department of Medicine, Jichi Medical School, Tochigi, Japan.
Hatake
K
K
Suzu
S
S
Harigaya
K
K
Miura
Y
Y
Motoyoshi
K
K
D001854:Bone Marrow Cells; D002460:Cell Line; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry*; D003094:Collagen / Q000378:metabolism; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000737:chemistry*; D008970:Molecular Weight; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D011508:Chondroitin Sulfate Proteoglycans; D014409:Tumor Necrosis Factor-alpha; D016173:Macrophage Colony-Stimulating Factor; D003094:Collagen