title: The nonapeptide leucinostatin A acts as a weak ionophore and as an immunosuppressant on T lymphocytes.
abstract: Earlier studies have disclosed that leucinostatin A, a hydrophobic nonapeptide antibiotic, assumes an alpha-helical secondary structure in nonpolar environments. The present report demonstrates that the peptide acts as a weak ionophore facilitating the transport of mono-and divalent cations through the plasma membrane of T lymphocytes and through artificial membranes. Leucinostatin A does not change the thymidine uptake of both resting mouse thymocytes and peripheral blood lymphocytes but dose-dependently prevents the activation of T lymphocytes by tetradecanoyl-phorbol-acetate and by anti-T cell receptor antibody.
authors.affiliation: Institute of Biochemistry I, Semmelweis University, School of Medicine, Budapest, Hungary.
authors.forename: P
authors.initials: P
authors.lastname: Csermely
authors.forename: L
authors.initials: L
authors.lastname: Radics
authors.forename: C
authors.initials: C
authors.lastname: Rossi
authors.forename: M
authors.initials: M
authors.lastname: Szamel
authors.forename: M
authors.initials: M
authors.lastname: Ricci
authors.forename: K
authors.initials: K
authors.lastname: Mihály
authors.forename: J
authors.initials: J
authors.lastname: Somogyi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism; D002414:Cations, Monovalent / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides; D013601:T-Lymphocytes / Q000187:drug effects*; D015032:Zinc / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D002413:Cations, Divalent; D002414:Cations, Monovalent; D007166:Immunosuppressive Agents; D007476:Ionophores; D010455:Peptides; C008268:leucinostatin A; D015032:Zinc; D002118:Calcium