Rapid detection of engraftment using T cell receptor gene polymorphism after allogeneic bone marrow transplantation in an alloimmunized child with severe aplastic anemia.
A severely alloimmunized boy with aplastic anemia received an HLA-identical BMT from his brother. Despite intensive immunosuppression and large marrow dose, peripheral signs of engraftment occurred only late under G-CSF treatment. With leukocyte counts of < 0.5 x 10(9)/l, chimerism could be proven not only by oligonucleotide fingerprinting but also within 48 h by analysis of polymorphism in the TCR gene family. This rapid and sensitive method to detect engraftment before it became quantitatively evident was important for the clinical management of the patient, obviating the need to search for an alternative marrow donor.
Kremens
B
B
Department of Pediatrics, University of Essen, Germany.
Gomolka
M
M
Ottinger
H
H
Grosse-Wilde
H
H
Schaefer
U W
UW
Epplen
J T
JT
D000741:Anemia, Aplastic / Q000235:genetics / Q000276:immunology* / Q000601:surgery*; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002675:Child, Preschool; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics; D006085:Graft Survival / Q000235:genetics / Q000276:immunology; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D014184:Transplantation, Homologous
D017931:DNA Primers; D004276:DNA, Satellite; D016693:Receptors, Antigen, T-Cell, alpha-beta