Development of a gene cloning system for Streptomyces hygroscopicus subsp. yingchengensis, a producer of three useful antifungal compounds, by elimination of three barriers to DNA transfer.
Streptomyces hygroscopicus 10-22 could not be transformed with any of the commonly used Streptomyces plasmid vectors and was resistant to plaque formation by the Streptomyces phages phi C31 and R4. Repeated selection resulted in the isolation of derivatives of S. hygroscopicus 10-22 that could be transformed with pIJ101- and pJV1-derived cloning vectors and of restriction-deficient derivatives that could accept DNA propagated in Streptomyces lividans 66. These new strains, which include three that still produce the original antibiotics, can be used as hosts for gene cloning. Insertion of nonreplicating vectors by homologous recombination and transposition of Tn4560 were demonstrated in S. hygroscopicus 10-22.
Qin
Z
Z
Department of Soil Sciences and Agrochemistry, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.
Peng
K
K
Zhou
X
X
Liang
R
R
Zhou
Q
Q
Chen
H
H
Hopwood
D A
DA
Kieser
T
T
Deng
Z
Z
D000935:Antifungal Agents / Q000096:biosynthesis; D001435:Bacteriophages; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial / Q000235:genetics*; D005822:Genetic Vectors; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D013302:Streptomyces / Q000235:genetics*; D014170:Transformation, Genetic
D000935:Antifungal Agents; D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes