BQ-123 inhibits both endothelin 1 and endothelin 3 mediated C6 rat glioma cell proliferation suggesting an atypical endothelin receptor.
The mitogenic action of endothelins (ETs) 1 and 3 was studied on C6 rat glioma cells in serum-free culture conditions. In order to characterize the ET receptor subtype involved in this effect, BQ-123, and ETA receptor selective antagonist was used. Our results confirmed that both ET-1 and ET-3 are mitogenic peptides for C6 cells and demonstrated for the first time that the ETA receptor antagonist BQ-123 inhibits the proliferative effect of both ET-1 and ET-3 in this cellular system, providing evidence of an atypical ET receptor on C6 cells.
Sedo
1st Department of Medicial Chemistry and Biochemistry, 1st Medical Faculty, Charles University, Prague.
Provero
P
P
Revoltella
R P
RP
Di Bartolo
V
V
Beffy
P
P
Mizrahi
J
J
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D065128:Endothelin Receptor Antagonists; D016232:Endothelins / Q000037:antagonists & inhibitors*; D005910:Glioma / Q000737:chemistry / Q000473:pathology*; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000494:pharmacology*; D051381:Rats; D017466:Receptors, Endothelin / Q000032:analysis*
D065128:Endothelin Receptor Antagonists; D016232:Endothelins; D010456:Peptides, Cyclic; D017466:Receptors, Endothelin; C072247:cyclo(Trp-Asp-Pro-Val-Leu)