title: [Isolation, purification, and comparative study of the properties of adenosine deaminase from five regions of the cattle brain].
abstract: Adenosine deaminase from the white and gray matter of the large hemispheres, cerebellum, medulla oblongata and pituitary anterior lobe has been isolated and purified. The pH optimum, Km, molecular mass, yield and specific activities for all the enzyme preparations have been determined. Gel filtration and electrophoresis data point to the heterogeneity of the enzyme. The lack of effects of SH-reagents suggests the absence of an essential SH-group. Among five bivalent metal ions, only Cu2+ irreversibly inhibited the enzyme activity in all the preparations.
authors.forename: S G
authors.initials: SG
authors.lastname: Sharoian
authors.forename: A A
authors.initials: AA
authors.lastname: Antonian
authors.forename: S S
authors.initials: SS
authors.lastname: Mardanian
mesh_terms: D000243:Adenosine Deaminase / Q000302:isolation & purification* / Q000378:metabolism; D058892:Adenosine Deaminase Inhibitors; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002384:Catalysis; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D008970:Molecular Weight
chemical_list: D058892:Adenosine Deaminase Inhibitors; D008670:Metals; D000243:Adenosine Deaminase
vernacular_title: Vydelenie, ochistka i sravnitel'noe izuchenie svoĭstv adenozindezaminazy iz piati otdelov mozga krupnogo rogatogo skota.