Detection of oncogenic HPV DNA by a consensus polymerase chain reaction method in genital carcinomas in twenty women in Barbados.
Paraffinized tissue from Barbadian women with histologically proven genital carcinoma was subjected to a consensus polymerase chain reaction method. Nineteen patients had cervical and one, vaginal carcinoma. The histological types were 17 squamous cell carcinoma, 2 adenocarcinoma and 1 adenosquamous carcinoma. HPV DNA was detected in 18/20 (90%). HPV DNA type 16 in 13 (65%), type 33 and type 45 in 1 (5%) each and 3 (15%) could not be typed. HPV DNA, type 16, was detected in one (50%) of the two cases of adenocarcinoma and 12/17 (71%) cases of squamous cell carcinoma. DNA HPV, type 33, and type 45 were each detected in 1/17 (6%) cases of squamous cell carcinoma. No HPV DNA, type 18, was detected.
Prussia
P R
PR
Department of Pathology and Surgery, U.W.I., Barbados.
ter Schegget
J
J
Smits
H L
HL
D000230:Adenocarcinoma / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D018196:Carcinoma, Adenosquamous / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D015344:DNA Probes, HPV / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D014625:Vaginal Neoplasms / Q000235:genetics* / Q000382:microbiology / Q000473:pathology
D015344:DNA Probes, HPV; D004279:DNA, Viral