Analysis of schizont antigen of Leucocytozoon caulleryi by SDS-PAGE and western blot.
To investigate the molecular weight of the schizont antigen of Leucocytozoon caulleryi, the schizont antigen was analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot. Thirty to 35 protein bands were detected by SDS-PAGE under reduced condition and ranged from 25 to 300 kDa. Most of them reacted with the sera of infected chickens by Western blot. The bands corresponding to polypeptides of 44, 58, 67 and 180 kDa reacted strongly, however, the bands of 30, 50 and 78 kDa did not react.
Isobe
T
T
Poultry Disease Laboratory, National Institute of Animal Health, Gifu, Japan.
Shimizu
S
S
Yoshihara
S
S
Suzuki
K
K
D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry*; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D056890:Eukaryota / Q000737:chemistry* / Q000254:growth & development; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000032:analysis*
D000953:Antigens, Protozoan; D015800:Protozoan Proteins