Diagnosis of human immunodeficiency virus (HIV) infection: multicenter evaluation of a newly developed anti-HIV 1 and 2 enzyme immunoassay.
A new anti-human immunodeficiency virus type 1 and 2 (anti-HIV 1 and 2) test is described. It uses recombinant p24 and peptides covering gp32, gp41, and gp120 to identify HIV-1 and HIV-2 infections. This test has been shown to be specific (99.5%) and sensitive (99.8%). In this respect, the assay was equal or superior to anti-HIV 1 and 2 tests run as references. The test was able to discriminate sera from patients with HIV infections from those from uninfected individuals with excellence; it also exerted high intra- and interassay precisions. The "modular" concept of the test allows the use of single components (gp32 or gp41) to separate between HIV-2 and HIV-1 infections, respectively.
Hess
G
G
Medizinische Klinik und Poliklinik, Klinikum der Johannes-Gutenberg-Universität, Mainz, Germany.
Avillez
F
F
Lourenco
M H
MH
D'Agostino
F
F
Cambie
G
G
Piot
P
P
Vercauteren
G
G
Michl
U
U
Melchior
W
W
Bayer
H
H
D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D005260:Female; D015483:HIV Antibodies / Q000097:blood; D015488:HIV Antigens; D015658:HIV Infections / Q000175:diagnosis* / Q000276:immunology; D006679:HIV Seropositivity / Q000175:diagnosis / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D008297:Male; D011247:Pregnancy; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity
D015483:HIV Antibodies; D015488:HIV Antigens; D011994:Recombinant Proteins