Effects of recombinant human cytokines on mitogen-induced bovine peripheral blood mononuclear cell proliferation.
The effects of interleukin 1 alpha (IL-1 alpha), IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, and insulin growth factor-1 (IGF-1) on proliferation of mitogen-stimulated bovine peripheral blood mononuclear cells (PBMC) were determined. Four concentrations of each cytokine were incubated 48 or 96 h with PBMC alone or with PBMC and three concentrations of three different mitogens. The mitogens included concanavalin A (Con A) phytohemagglutinin (PHA), and Escherichia coli 055:B5 lipopolysaccharide (LPS). Proliferation of unstimulated PBMC was not affected by IL-1 alpha, IL-1 beta, IL-3, IL-5, IL-6, IL-8, and IGF-1 whereas IL-2, IL-4, and IL-7 increased proliferation. Incubation of PBMC with Con A plus IL-1 alpha, IL-2, IL-4, IL-7 or IL-8 increased proliferation when compared to PBMC that were incubated with either Con A or the cytokine alone. Interleukin-1 beta, IL-3, IL-5, IL-6 and IGF-1 did not influence proliferation of Con A-stimulated PBMC. Proliferation of PHA-stimulated PBMC was increased when PBMC were cultured with IL-2, IL-4 or IL-7, but not when cultured with IL-1 alpha, IL-1 beta, IL-3, IL-5, IL-6, IL-8 or IGF-1. Similar results occurred with LPS-stimulated PBMC in that proliferation induced by LPS was enhanced by IL-2 or IL-7, but not by any of the other cytokines.(ABSTRACT TRUNCATED AT 250 WORDS)
Olsen
S C
SC
National Animal Disease Center, USDA, Ames, IA 50010.
Stevens
M G
MG
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007378:Interleukins / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology
D007378:Interleukins; D008070:Lipopolysaccharides; D008934:Mitogens; D010835:Phytohemagglutinins; D011994:Recombinant Proteins; D003208:Concanavalin A; D007334:Insulin-Like Growth Factor I