[Normal values for hematological and hemostatic parameters in the rabbit. Determination of new parameters for experimental models of thrombosis and hemostasis].
OBJECTIVE
Assessment of normal haematological values in the rabbit using a semi-automatic analyser. Evaluation of new haemostatic parameters necessary for experimental models in thrombosis and haemostasis.


METHODS
20 New Zealand male rabbits of 2-2.5 kg were anaesthetized and blood was collected from the jugular vein. The following haematological determinations were carried out using a semi-automatic analyser: red blood cells count, haemoglobin, haematocrit, indexes, white blood cells count, platelet count and mean platelet volume. The parameters used to assess the physiological haemostasis in rabbit were: bleeding time, fibrinogen, prothrombin time, activated partial thromboplastin time, thrombin time; and others not yet determined in the rabbit: antithrombin III, thrombin-antithrombin complexes and F1+2 fragments to measure the basal thrombin-formation activity, and fibrin D-dimer and fibrinogen degradation products for fibrinolysis activity.


RESULTS
The haematological results obtained were similar than with other techniques, but with no stress influence. Normal values for haemostatic parameters studied are shown, including the parameters for the determination of physiological thrombin-formation and fibrinolysis activity. F1+2 fragments and fibrinogen degradation products could not be evaluated.


CONCLUSIONS
The haematological results obtained were completely normal. All the haemostatic parameters studied were found useful to determine the physiological activity of coagulation and fibrinolysis in the rabbit, and specially for experimental models of hypercoagulability and hyperfibrinolisys states.
Monreal
L
L
Unidad de Trombosis Experimental, Universidad Autónoma de Barcelona.
Anglés
A M
AM
Ruiz de Gopegui
R
R
Espada
Y
Y
Monasterio
J
J
Roncalés
F J
FJ
Monreal
M
M
D000818:Animals; D001772:Blood Cell Count; D001780:Blood Coagulation Tests; D001798:Blood Proteins / Q000032:analysis; D004909:Erythrocyte Indices; D005342:Fibrinolysis; D006487:Hemostasis; D008297:Male; D011817:Rabbits / Q000097:blood*; D012016:Reference Values; D013312:Stress, Physiological / Q000097:blood; D013927:Thrombosis / Q000097:blood*
D001798:Blood Proteins
Valores normales de los parámetros hematológicos y hemostáticos en el conejo. Determinación de nuevos parámetros para modelos experimentales de trombosis y hemostasia.