A high-performance liquid chromatographic method for the simultaneous determination of venlafaxine and O-desmethylvenlafaxine in biological fluids.
A rapid, accurate, and sensitive high-performance liquid chromatographic (HPLC) method for simultaneous determination of venlafaxine (V) and O-desmethylvenlafaxine (ODV) in plasma and urine has been developed. V and ODV are extracted from plasma using a liquid-liquid extraction procedure, chromatographed on a Supelcosil LC-8DB column, and quantitated by UV detection at 229 nm. Linearity was established over the range 10-500 ng/ml for V and 7.2-720 ng/ml for ODV using 1.0 ml of human, rat, dog, and mouse plasma. For urine, for both analytes, an analytical range 0.1-10.0 micrograms/ml was established. Accuracy of > +/- 10% about the theoretical mean was achieved for all matrices, with intra- and interday coefficients of variation for precision of < 10%. Endogenous components in plasma and/or urine or known metabolites of V do not interfere in the determination of the analytes. For both V and ODV a quantitation limit of 10 ng/ml for plasma was adequate for their estimation over a period of three half-lives, following administration of a pharmacologic dose in man, and the limit of 0.1 microgram/ml, for urine, can monitor excretion of as little as 0.5% of the dose.
Hicks
D R
DR
Drug Metabolism Division, Wyeth-Ayerst Research, Princeton, New Jersey 08540.
Wolaniuk
D
D
Russell
A
A
Cavanaugh
N
N
Kraml
M
M
D000818:Animals; D000928:Antidepressive Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003511:Cyclohexanols / Q000032:analysis* / Q000097:blood / Q000652:urine; D000069468:Desvenlafaxine Succinate; D004285:Dogs; D006207:Half-Life; D006801:Humans; D007202:Indicators and Reagents; D051379:Mice; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D000069470:Venlafaxine Hydrochloride
D000928:Antidepressive Agents; D003511:Cyclohexanols; D007202:Indicators and Reagents; D000069470:Venlafaxine Hydrochloride; D000069468:Desvenlafaxine Succinate