The role of central corticoliberin in the ether stress-induced secretion of neurohypophyseal hormones and corticosterone in the rat.
As corticotropin-releasing factor (CRF) and oxytocin (OXT) are released in response to various stressors and a role of CRF in stress-induced OXT secretion has been proposed by previous authors, the present experiments were scheduled to investigate the participation of the brain CRF system in the stress-evoked release of OXT, arginine-8-vasopressin (AVP) and corticosterone. CRF-antiserum (AS) was given into the lateral ventricle of the brain of Wistar male rats, and 24 h later, the injection was repeated 30 min prior to ether stress followed by decapitation in 5 min. Plasma OXT and AVP were measured by radioimmunoassay and corticosterone by fluorimetry. Ether stress increased the levels of corticosterone and OXT, but not that of AVP. CRF-AS alone did not change the secretion of these hormones. CRF-AS pretreatment blocked the corticosterone-releasing action of ether stress, whereas it exerted no influence on the stress-induced OXT secretion into the circulation. There was no effect of a combined application of CRF-AS and stress on the plasma AVP level. These results suggest that the central CRF system is involved in the ether stress-elicited corticosterone response, however CRF is unlikely to be connected with the regulation of OXT secretion under these experimental conditions.
Laczi
F
F
Endocrine Unit of First Department of Medicine, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Iványi
T
T
Sarnyai
Z
Z
Vecsernyés
M
M
Lengyel
G
G
Szabó
G
G
Bíró
E
E
Gardi
J
J
Julesz
J
J
Telegdy
G
G
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D003345:Corticosterone / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D004986:Ether / Q000633:toxicity*; D007106:Immune Sera / Q000494:pharmacology; D007276:Injections, Intraventricular; D008297:Male; D010121:Oxytocin / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000187:drug effects* / Q000378:metabolism; D010913:Pituitary-Adrenal System / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012636:Secretory Rate / Q000187:drug effects; D013312:Stress, Physiological / Q000139:chemically induced / Q000503:physiopathology*
D007106:Immune Sera; D004986:Ether; D001127:Arginine Vasopressin; D010121:Oxytocin; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone