title: Macrophage treatment in suckling rat endotoxic shock.
abstract: Gram-negative sepsis/septic shock in the human newborn continues to be a severe medical problem because of significant mortality and morbidity. Since macrophages detoxify endotoxin, a decreased number of macrophages may contribute to the newborn's sensitivity to endotoxin. In this study, peritoneal macrophages were used for the treatment of endotoxic shock in 10-day-old rats, and 24-hr mortality, plasma glucose, and lactate concentrations were monitored. Peritoneal macrophages were harvested from adult or 10-day-old rats. Caseinate-stimulated macrophages from adult and 10-day-old rats significantly decreased the mortality of 10-day-old rat endotoxic shock from 90% to 37.5% and 44.4%, respectively. Resident macrophages from adult and 10-day-old rats also decreased the mortality from 90% to 12.5% and 45.4%, respectively. Peritoneal macrophages from adult rats significantly ameliorated hypoglycemia during endotoxic shock in a dose-dependent manner. Macrophage treatment decreased plasma endotoxin concentration (P < 0.05). Macrophage treatment was important for host defense.
authors.affiliation: Department of Pediatrics, Stritch School of Medicine, Loyola University of Chicago, Maywood, Illinois.
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.forename: R C
authors.initials: RC
authors.lastname: Lichtenberg
authors.forename: M E
authors.initials: ME
authors.lastname: Gottschalk
authors.forename: C L
authors.initials: CL
authors.lastname: Anderson
authors.forename: H L
authors.initials: HL
authors.lastname: Mathews
authors.forename: W P
authors.initials: WP
authors.lastname: Zeller
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D001786:Blood Glucose / Q000032:analysis; D002452:Cell Count; D005260:Female; D007773:Lactates / Q000097:blood; D008070:Lipopolysaccharides / Q000097:blood / Q000633:toxicity; D008264:Macrophages / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000628:therapy*
chemical_list: D001786:Blood Glucose; D007773:Lactates; D008070:Lipopolysaccharides