Increase of c-jun mRNA upon hypo-osmotic cell swelling of rat hepatoma cells.
c-jun mRNA levels were increased in rat hepatoma cells (H4-II-E-C3) when exposed to hypotonic medium (205 mosmol/l) with a maximal induction observed after 1 h of hypotonic exposure. At this time point an approximate 5-fold increase in c-jun expression could be detected in relation to normotonic control incubations (305 mosmol/l). Hypertonic exposure (405 mosmol/l) had only a slight effect on c-jun expression. In contrast to the increased c-jun mRNA levels under hypotonic conditions, expression of the c-fos proto-oncogene was unaffected by changes in the osmolarity. The hypotonicity-induced increase in c-jun expression was also detectable in the presence of a protein kinase C (PKC) inhibitor. This indicates that PKC is not involved in the signal transduction pathway leading to c-jun expression upon hypotonic cell swelling in these cells.
Finkenzeller
G
G
Medizinische Universitätsklinik Freiburg, Germany.
Newsome
W
W
Lang
F
F
Häussinger
D
D
D000818:Animals; D048429:Cell Size; D004789:Enzyme Activation; D007211:Indoles / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental; D008301:Maleimides / Q000494:pharmacology; D009994:Osmolar Concentration; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate; D014407:Tumor Cells, Cultured
D007211:Indoles; D008301:Maleimides; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D011493:Protein Kinase C; C070515:bisindolylmaleimide I; D013755:Tetradecanoylphorbol Acetate