Protein motif by computer: the perfect Greek key jellyroll designer.
A program that generates amino acid sequences that are compatible with the Greek key protein motif is presented. Using statistical data derived from the structures of molecules from the protein databank, the novel algorithm generates amino acid sequences compatible with an 8-stranded perfect Greek key jellyroll motif. In this motif, all hydrogen bonds present in the theoretical originating beta-hairpin stay in register as the whole 8-stranded domain folds at once in an anticlockwise swirl. Eight residues are generated per strand and 32 residues per sheet making 64 residues in the antiparallel beta-barrel. The seven loops between beta-strands contain an additional 27 residues. All recognized features of beta-sheets and beta-strands, such as alternating hydrophobic, hydrophilic residues with hydrophobics on the narrow-hydrogen-bond-pair, concave side of the theoretical originating beta-ribbon; sheet twist, strand twist, side chain rotation about the strands; the theories of side chain packing between the sheets; an average 30 degrees rotation between beta-sheets; the theoretical anticomplementary patch residues of each sheet; and the anticomplementary, isotropically stressed hyperbolic parabloid shape of each sheet, are taken into account in the program. The sequences of the loops between strands are designed by turn type and strand twist is considered in the design of the motif's single beta-hairpin turn. Secondary structure parameters and between-strand amino acid pair correlations also figure importantly in the novel algorithm.
Henneke
C M
CM
Department of Protein Engineering, Agricultural and Food Research Council Institute of Food Research, Reading Laboratory, UK.
D000465:Algorithms; D000595:Amino Acid Sequence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D012984:Software
D011506:Proteins