Stimulation of gastrointestinal antibody to Shiga toxin by orogastric immunization in mice.
Shiga toxin (ST) is a protein toxin of Shigella dysenteriae type 1, a causative agent of severe diarrhoea and dysentery. In this report we describe the gastrointestinal secretory antibody response of mice following orogastric immunization with ST. Gastrointestinal secretions were sampled by a gastrointestinal lavage technique weekly for 5 weeks after initial immunization. Assay of lavage samples by ELISA showed that mice vaccinated orogastrically with various doses of ST developed gastrointestinal antibody to ST in a dose-dependent manner. Serum anti-ST activity developed by 5 weeks after initial immunization. The ability of ST to act as a mucosal immune adjuvant was investigated by coadministration of ST and keyhole limpet haemocyanin. In contrast to cholera toxin, a potent adjuvant, ST did not demonstrate adjuvant activity. The mouse gastrointestinal lavage model could be useful for further analysis of the cellular basis of ST immunogenicity.
Suckow
M A
MA
Unit for Laboratory Animal Medicine, University of Michigan Medical School, Ann Arbor.
Keren
D F
DF
Brown
J E
JE
Keusch
G T
GT
D000276:Adjuvants, Immunologic; D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology; D003603:Cytotoxins / Q000276:immunology; D004064:Digestive System / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D007109:Immunity; D051379:Mice; D008810:Mice, Inbred C57BL; D022603:Shiga Toxins; D012762:Shigella dysenteriae / Q000276:immunology
D000276:Adjuvants, Immunologic; D001323:Autoantibodies; D001427:Bacterial Toxins; D003603:Cytotoxins; D022603:Shiga Toxins; D002772:Cholera Toxin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin