title: A cell surface protein that binds avian hepatitis B virus particles.
abstract: We have identified a 180-kDa cellular glycoprotein (gp180) that binds with high affinity to duck hepatitis B virus (DHBV) particles. The protein was detected by coprecipitating labeled duck hepatocyte proteins with virions or recombinant DHBV envelope proteins, using nonneutralizing monoclonal antibodies to the virion envelope. Binding of gp180 requires only the pre-S region of the viral large envelope protein, since recombinant fusion proteins bearing only this region efficiently coprecipitate gp180. The DHBV-gp180 interaction is blocked by two independent neutralizing monoclonal antibodies. The protein is found on both internal and surface membranes of the cell, and the species distribution of gp180 binding activity mirrors the known host range of DHBV infection. Functional gp180 is expressed in a wide variety of tissues in susceptible ducks.
authors.affiliation: Department of Microbiology and Immunology, University of California Medical Center, San Francisco 94143.
authors.forename: K
authors.initials: K
authors.lastname: Kuroki
authors.forename: R
authors.initials: R
authors.lastname: Cheung
authors.forename: P L
authors.initials: PL
authors.lastname: Marion
authors.forename: D
authors.initials: D
authors.lastname: Ganem
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000494:pharmacology; D002268:Carboxypeptidases / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry*; D004372:Ducks; D006023:Glycoproteins; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000378:metabolism*; D004371:Hepatitis Virus, Duck / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins; D009500:Neutralization Tests; D011485:Protein Binding / Q000187:drug effects; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011506:Proteins; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity; D014018:Tissue Distribution
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D006514:Hepatitis B Surface Antigens; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011498:Protein Precursors; D011506:Proteins; D011994:Recombinant Proteins; D002268:Carboxypeptidases; C094315:metallocarboxypeptidase D