Administration of amyloid beta-peptides into the medial septum of rats decreases acetylcholine release from hippocampus in vivo.
The septum of male Wistar rats was injected with synthetic beta-amyloid fragments, beta 12-28, beta 25-35 and beta 1-40, and hippocampal acetylcholine (ACh) release was evaluated by transversal microdialysis. A marked decrease in basal and K(+)-evoked ACh release was found 7 or 21 days after injection of 5 nmol of beta 12-28 and beta 25-35, or 3 nmol of beta 1-40, respectively. These data indicate that septal injection of beta-amyloid peptides causes hypofunction of the septo-hippocampal cholinergic system.
Abe
E
E
Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
Casamenti
F
F
Giovannelli
L
L
Scali
C
C
Pepeu
G
G
D000109:Acetylcholine / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001921:Brain; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003224:Congo Red; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D006651:Histocytochemistry; D007267:Injections; D008297:Male; D017551:Microdialysis; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D003224:Congo Red; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine; D011188:Potassium