[Establishment and characterization of a CA125-producing human ovarian clear cell carcinoma cell line].
A human ovarian cancer cell line designated "KK" was established from ascites of a patient with ovarian clear cell carcinoma. This cell line was grown for more than 2 years and over 140 passages in medium RPMI1640 containing 10% FCS. Doubling time of this cell line at passage 70 was approximately 4 days and saturation density was 1.1 x 10(5)/cm2. Plating efficiency was approximately 23%. Chromosome analysis revealed aneuploidy with a model number of 67. PAS-positive substances were present in the cytoplasm. CA125 and SLX were detected in both the original tumor and the cultured cells. This cell line is less sensitive to cisplatin than KF cells and IC50 was 0.95 microM.
Sasa
H
H
Department of Obstetrics and Gynecology, National Defense Medical College, Tokorozawa, Japan.
Ishii
K
K
Hirata
J
J
Kikuchi
Y
Y
Nagata
I
I
Kawai
T
T
Senoo
A
A
Sugita
M
M
Sugishita
T
T
Tenjin
Y
Y
D018262:Adenocarcinoma, Clear Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000096:biosynthesis*; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D008938:Mitosis; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*
D015295:Antigens, Tumor-Associated, Carbohydrate; D002945:Cisplatin