title: Multiple cis-acting targeting sequences are required for orb mRNA localization during Drosophila oogenesis.
abstract: The targeting of positional information to specific regions of the oocyte or early embryo is one of the key processes in establishing anterior-posterior and dorsal-ventral polarity. In many developmental systems, this is accomplished by localization of mRNAs. The germ line-specific Drosophila orb gene plays a critical role in defining both axes of the developing oocyte, and its mRNA is localized in a complex pattern during oogenesis. We have identified a 280-bp sequence from the orb 3' untranslated region capable of reproducing this complex localization pattern. Furthermore, we have found that multiple cis-acting elements appear to be required for proper targeting of orb mRNA.
authors.affiliation: Department of Biology, Washington University, St. Louis, Missouri 63130.
authors.forename: V
authors.initials: V
authors.lastname: Lantz
authors.forename: P
authors.initials: P
authors.lastname: Schedl
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis; D010053:Ovary / Q000502:physiology; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D001616:beta-Galactosidase