Incorporation of (R)- and (S)-3',4'-seco-thymidine into oligodeoxynucleotides: hybridization properties and enzymatic stability.
Novel flexible oligodeoxynucleotide analogues containing (R)- and (S)-3',4'-seco-thymidine were synthesized on an automated DNA-synthesizer using the phosphoramidite approach. Oligodeoxynucleotide analogues (17-mers) having one or three modifications in the middle or one or two modifications in the ends were evaluated with respect to hybridization properties and enzymatic stability. 3'-End-modified oligomers were stable towards 3'-exonuclease degradation and displayed acceptable hybridization properties.
Nielsen
P
P
Department of Chemistry, Odense University, Denmark.
Kirpekar
F
F
Wengel
J
J
D001483:Base Sequence; D004795:Enzyme Stability; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D013936:Thymidine / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; D013936:Thymidine