title: Preparation of human rabies vaccine in VERO cell culture using a microcarrier system.
abstract: 1. The rabies virus (Pasteur PV strain) was propagated in VERO cells attached to microcarriers in a 3.7-1 bioreactor. Virus titers of about 10(6) LD50/ml were obtained regularly. 2. Ultrafiltration was efficient for concentrating the virus suspensions, and the sucrose gradient reduced the residual VERO cell DNA to acceptable levels (less than 50 pg/dose). The remaining cell DNA content was evaluated by dot-blot hybridization with a probe prepared with VERO cell DNA. 3. The final virus preparations were inactivated by B-propiolactone treatment, showed a potency higher than 2.5 IU/dose and protected mice experimentally infected intracerebrally with rabies virus (CVS-13.2). 4. This methodology for the production of a rabies vaccine for human use should be of interest to countries where high technology facilities are not available.
authors.affiliation: Instituto Butantan, São Paulo, Brasil.
authors.forename: R Z
authors.initials: RZ
authors.lastname: Mendonça
authors.forename: L M
authors.initials: LM
authors.lastname: Ioshimoto
authors.forename: R M
authors.initials: RM
authors.lastname: Mendonça
authors.forename: M
authors.initials: M
authors.lastname: De-Franco
authors.forename: E J
authors.initials: EJ
authors.lastname: Valentini
authors.forename: W
authors.initials: W
authors.lastname: Beçak
authors.forename: I
authors.initials: I
authors.lastname: Raw
authors.forename: C A
authors.initials: CA
authors.lastname: Pereira
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D004247:DNA / Q000032:analysis; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D009693:Nucleic Acid Hybridization; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000254:growth & development / Q000276:immunology*; D013997:Time Factors; D015164:Vaccines, Inactivated / Q000276:immunology; D014709:Vero Cells
chemical_list: D000914:Antibodies, Viral; D011819:Rabies Vaccines; D015164:Vaccines, Inactivated; D004247:DNA