Zinc hydroxide induced respiratory burst in rat neutrophils.
The effects of zinc hydroxide on the respiratory burst and phagocytosis by rat neutrophils were examined. Zinc hydroxide induced an increase in oxygen consumption and O2- production. Electronmicroscopy showed that neutrophils engulfed zinc hydroxide particles by phagocytosis. Pertussis toxin (0.25, 0.5, 1.0 micrograms/ml) and EGTA (1, 2, 5 mM) inhibited zinc hydroxide-induced O2- production in a dose-dependent manner. The inhibitors of protein kinase C, 1-(5-isoquinolinesulfonyl)-2-methyl-piperazine and N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide inhibited zinc hydroxide-induced O2- production with IC50 values ranging between 10 microM and 25 microM. The inhibitory study using an inhibitor of myosin light chain kinase, 1-(5-iodo-naphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine, showed IC50 values ranging from 5 microM to 10 microM. These findings indicate that zinc hydroxide induces respiratory burst and phagocytosis by rat neutrophils.
Ogino
K
K
Department of Public Health, Yamaguchi University School of Medicine, Ube, Japan.
Izumi
Y
Y
Segawa
H
H
Takeyama
Y
Y
Ishiyama
H
H
Houbara
T
T
Uda
T
T
Yamashita
S
S
D000818:Animals; D006878:Hydroxides / Q000494:pharmacology*; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D009504:Neutrophils / Q000187:drug effects* / Q000473:pathology; D010101:Oxygen Consumption / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D016897:Respiratory Burst / Q000187:drug effects*; D013481:Superoxides / Q000032:analysis; D017967:Zinc Compounds / Q000494:pharmacology*
D006878:Hydroxides; D017967:Zinc Compounds; D013481:Superoxides; C052745:zinc hydroxide