Growth and metabolism of cultured bone cells using microcarrier and monolayer techniques.
The traditional in vitro cell culture methods provide bone cells on matrix-coated Petri dishes to grow the cells in a monolayer. This limits the usefulness in situations where there is a need to suspend anchorage dependent cells. To promote the massive production of the cultured cells and to enable the suspension of the cells in a medium, microcarrier cell culture was developed and evaluated. Isolated bone cells from rat calvaria were incubated in a microcarrier culture flask with Cytodex 1. The microcarrier cell morphology was examined by a phase contrast microscope, a scanning electron microscope, and a transmission electron microscope. Cell growth, enzyme markers, and biosynthetic characteristics were examined and compared for microcarrier and monolayer methods. The results indicate that all the characteristics of the bone cells, whether cultured in the Petri dish or microcarrier, were the same. Therefore, the studies of the function and behavior of bone cells still remain useful using the microcarrier system culture.
Tang
J S
JS
Department of Orthopaedic Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Chao
C F
CF
Au
M K
MK
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001846:Bone Development; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003584:Cytological Techniques; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D010011:Osteocytes / Q000378:metabolism* / Q000648:ultrastructure; D011509:Proteoglycans / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D011509:Proteoglycans; D003094:Collagen; D000469:Alkaline Phosphatase