[Measurement of human thyroid peroxidase autoantibodies by enzyme immunoassay using recombinant human TPO].
An EIA for measuring anti-TPO autoantibodies (rhTPO-EIA) was developed using recombinant human TPO expressed in CHO cells and was compared with MC-HA generally used in laboratory routine work. rhTPO-EIA showed a satisfactory reproducibility in the intra-assay test and did not have an accidental error of lots. Almost equal number of healthy females and males were measured for their IgG binding to TPO to define a normal range of anti-TPO autoantibodies. After setting 20 IU/ml as an upper limit of normal range, sera from patient with thyroid disorders were measured for their anti-TPO autoantibodies. Chronic thyroiditis and Graves' disease were highly positive, while adenoma, thyroid cancer, SLE, and RA were low in their positivity. The positive rate of anti-TPO autoantibodies was compatible to those of previous reports in each disorder. Seventy-two sera from patients with chronic thyroiditis or Graves' disease were measured for their autoantibodies by both rhTPO-EIA and MC-HA and the results were compared between both methods. A correlation coefficient was 0.486. Following absorption with thyroglobulin, sera were measured again and as the results, the correlation coefficient increased to 0.723. Therefore, MC-HA was thought to be influenced in the presence of anti-thyroglobulin autoantibodies. Since rhTPO-EIA is excellent in quality and not affected by anti-thyroglobulin antibodies, it is useful and applicable to clinical diagnosis and observation of thyroid disorders.
Inoue
T
T
Department of Laboratory Medicine, Miyazaki Medical College.
Ishiguro
R
R
Takenouchi
H
H
Umeki
K
K
Matsumoto
K
K
Yagihashi
S
S
Kato
H
H
Kotani
T
T
Ohtaki
S
S
D001323:Autoantibodies / Q000032:analysis*; D005260:Female; D006111:Graves Disease / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D007453:Iodide Peroxidase / Q000276:immunology*; D008297:Male; D011994:Recombinant Proteins; D015203:Reproducibility of Results; D013967:Thyroiditis, Autoimmune / Q000276:immunology
D001323:Autoantibodies; D011994:Recombinant Proteins; D007453:Iodide Peroxidase