title: DNA flow-cytometric analysis of basal cell carcinomas and its relevance to their morphological differentiation: a retrospective study.
abstract: DNA flow cytometry (FCM) was performed on archived material of 82 basal cell carcinomas (BCC), using Hedley's technique. Sixty-five samples were analysed. Biopsy samples were classified in five groups depending on their morphological differentiation: solid, cystic, adenoid, keratotic and metatypical. Two parameters were studied: proliferative index and DNA index. Statistical analysis revealed that BCC with keratinizing or metatypical differentiation had a greater amount of aneuploidy when compared with BCC without particular differentiation (solid and cystic) or aneuploid differentiation (p < 0.001). The proliferative indices were significantly different in the four groups. DNA FCM of BCC confirms the value of morphological classifications and gives another example of aneuploidy in a tumour with low aggressiveness.
authors.affiliation: Clinique Dermatologique, Centre Hospitalier Régional et Universitaire, Rouen, France.
authors.forename: M
authors.initials: M
authors.lastname: Fortier-Beaulieu
authors.forename: A
authors.initials: A
authors.lastname: Laquerrière
authors.forename: E
authors.initials: E
authors.lastname: Thomine
authors.forename: P
authors.initials: P
authors.lastname: Lauret
authors.forename: J
authors.initials: J
authors.lastname: Hémet
mesh_terms: D000782:Aneuploidy; D002280:Basal Cell Carcinoma / Q000737:chemistry / Q000145:classification / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000737:chemistry / Q000145:classification / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm