[Phospholipase A2 in the biochemical mechanisms of spleen lymphocyte reaction to x-ray irradiation of animals].
Preincubation of rat splenic lymphocytes with nonspecific mitogens and calcium ionophore A23187 led to the activity of phospholipase A2 which hydrolyzed arachidonoyl phospholipids and to increased cellular levels of free arachidonic acid. The effect of some substances was decreased as follows: A23187 > > lipopolysaccharide > phytohemagglutinin > concanavalin A. Dose-dependent activation of phospholipid hydrolysis and arachidonic acid release were observed within 6-12 hrs after X-ray irradiation of animals in doses of 0.5 and 1.0 Gy. The involvement of phospholipase A2 in deterioration of receptor interactions occurring during transmission of an activation signal in splenic lymphocytes in the early postirradiation period is discussed.
Matyshevskaia
O P
OP
Slatvinskaia
E A
EA
Kucherenko
N E
NE
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D006868:Hydrolysis; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D008934:Mitogens / Q000494:pharmacology; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*
D008934:Mitogens; D010743:Phospholipids; D000001:Calcimycin; D010741:Phospholipases A; D054467:Phospholipases A2
Fosfolipaza A2 v biokhimicheskikh mekhanizmakh reaktsii limfotsitov selezenki na rentgenovskoe obluchenie zhivotnykh.