[Pathogenic action of Erwinia herbicola. I. In vitro investigations].
The effects of antigen and endotoxin of Erwinia herbicola on physiological status of alveolar macrophages were studied in vitro. The studies comprised determination of the cytotoxicity of antigens + and their effects on superoxide anion and interleukin-1 (IL-1) generation by alveolar macrophages. It was found that tested agents were cytotoxic for alveolar macrophages in large concentrations (from 1000 micrograms/ml. In lower concentrations (1-10 micrograms/ml) they stimulated the activity of alveolar macrophages, causing significant increase in superoxide anion(O2) and IL-1 generation. Superoxide anion and IL-1 production may be of importance in pathogenesis of organic dust-induced lung diseases and our results indicate that agents associated with these diseases induced their production.
Milanowski
J
J
Kliniki i Katedry Chorób Płuc i Gruźlicy Akademii Medycznej, Lublinie.
Dutkiewicz
J
J
Fafrowicz
B
B
D000818:Animals; D004885:Erwinia / Q000472:pathogenicity*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis / Q000276:immunology; D016676:Macrophages, Alveolar / Q000276:immunology*; D013481:Superoxides / Q000276:immunology / Q000378:metabolism
D007375:Interleukin-1; D013481:Superoxides
Patogenne działanie pałeczki Erwinia herbicola. I. Badania in vitro.