Immunoglobulin prophylaxis against human T cell lymphotropic virus type II in rabbits.
Hyperimmune globulins, I-IgG and II-IgG, prepared from healthy persons seropositive for human T cell lymphotropic virus (HTLV) types I and II, respectively, were tested for their prophylactic effect against HTLV-II infection in rabbits. Three groups (A, B, and C) of 3 female rabbits each were used. Group A rabbits were inoculated intravenously with HTLV-II-carrying rabbit lymphoid cell line RII. Group B and C rabbits were immunized, respectively, with II-IgG and I-IgG and challenged with RII cells 24 h later. All group A and C rabbits seroconverted for HTLV-II after 2 weeks, but all group B rabbits were protected from HTLV-II infection. Gene amplification by polymerase chain reaction revealed the presence of HTLV-II provirus sequences in all group A and C rabbits but in none of the group B rabbits. These findings indicate that passive immunization with II-IgG is effective in preventing HTLV-II infection and that there is no cross-neutralization between HTLV-I and -II.
Morishita
N
N
Department of Medicine, Kochi Medical School, Japan.
Ishii
K
K
Tanaka
Y
Y
Sawada
T
T
Hoshino
H
H
Foung
S K
SK
Miyoshi
I
I
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D005260:Female; D015482:HTLV-II Antibodies / Q000276:immunology / Q000627:therapeutic use*; D015491:HTLV-II Infections / Q000276:immunology / Q000517:prevention & control*; D006801:Humans; D007074:Immunoglobulin G / Q000627:therapeutic use*; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D011817:Rabbits
D015482:HTLV-II Antibodies; D007074:Immunoglobulin G