title: Neutrophil activation by monomeric interleukin-8.
abstract: Interleukin-8 (IL-8), a pro-inflammatory protein, has been shown by nuclear magnetic resonance (NMR) and x-ray techniques to exist as a homodimer. An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectivity block formation of hydrogen bonds between monomers and thereby prevent dimerization. This analog was shown to be a monomer, as assessed by analytical ultracentrifugation and NMR. Nevertheless, it was equivalent to IL-8 in assays of neutrophil activation, which indicates that the monomer is a functional form of IL-8.
authors.affiliation: Protein Engineering Network of Centres of Excellence (PENCE), University of Alberta, Edmonton, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Rajarathnam
authors.forename: B D
authors.initials: BD
authors.lastname: Sykes
authors.forename: C M
authors.initials: CM
authors.lastname: Kay
authors.forename: B
authors.initials: B
authors.lastname: Dewald
authors.forename: T
authors.initials: T
authors.lastname: Geiser
authors.forename: M
authors.initials: M
authors.lastname: Baggiolini
authors.forename: I
authors.initials: I
authors.lastname: Clark-Lewis
mesh_terms: D002118:Calcium / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D006801:Humans; D006860:Hydrogen Bonding; D016209:Interleukin-8 / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D019272:Leukocyte Elastase; D008956:Models, Chemical; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010196:Pancreatic Elastase / Q000378:metabolism; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D018123:Receptors, Interleukin / Q000737:chemistry / Q000378:metabolism; D023062:Receptors, Interleukin-8A
chemical_list: D016209:Interleukin-8; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D002118:Calcium