title: Characterisation of the external surfaces of Pseudomonas aeruginosa isolated from human blood and respiratory tract.
abstract: The surface structures of the cell envelopes of 16 clinical isolates of Pseudomonas aeruginosa were examined by electronmicroscopy with the new fixation technique of freeze-substitution. Two types of structures were observed among the organisms. In one group of strains, mostly isolated from blood, a dense fibrous layer c. 30 nm thick was found around the outer-membrane surface, whereas no such structure was observed in the other group of isolates, most of which were from sputum. Lipopolysaccharides extracted from the isolates with a dense fibrous layer were found by SDS-PAGE to have long O-polysaccharide chains, whereas strains without such a layer mostly had lipopolysaccharides that lacked high mol. wt. O-polysaccharide chains.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Misumi
authors.forename: A
authors.initials: A
authors.lastname: Umeda
authors.forename: K
authors.initials: K
authors.lastname: Eguchi
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: Y
authors.initials: Y
authors.lastname: Sawae
authors.forename: Y
authors.initials: Y
authors.lastname: Niho
authors.forename: K
authors.initials: K
authors.lastname: Amako
mesh_terms: D016470:Bacteremia / Q000382:microbiology*; D001770:Blood Bactericidal Activity; D004591:Electrophoresis, Polyacrylamide Gel; D005615:Freezing; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000032:analysis / Q000737:chemistry*; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000648:ultrastructure*; D012141:Respiratory Tract Infections / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology
chemical_list: D008070:Lipopolysaccharides