Effect of freeze-dried poly-L-lactic acid discs mixed with bone morphogenetic protein on the healing of rat skull defects.
Porous poly-L-lactic-acid discs were prepared by a freeze-drying method (FDPLLA). During preparation of the discs, semipurified bone morphogenetic protein (BMP) was mixed with the poly-L-lactic acid (500 micrograms BMP per disc). The discs were implanted in the skull defects of rats and retrieved after 2 or 4 weeks. With the FDPLLA/BMP discs, new bone formation was observed at 2 weeks after implantation. On the other hand, plain FDPLLA discs failed to produce new bone formation even at 4 weeks after implantation. Calcium content of the retrieved FDPLLA/BMP discs was statistically higher than that of plain FDPLLA discs. These results suggest that FDPLLA may be an effective delivery system for BMP and that the FDPLLA/BMP disc may potentially have usefulness as a bone graft substitute.
Miki
T
T
Second Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Harada
K
K
Imai
Y
Y
Enomoto
S
S
D000818:Animals; D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D002118:Calcium / Q000378:metabolism; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D006133:Growth Substances / Q000008:administration & dosage*; D007773:Lactates; D019344:Lactic Acid; D008297:Male; D010012:Osteogenesis; D011091:Polyesters; D011108:Polymers; D019736:Prostheses and Implants; D011506:Proteins / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000601:surgery
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D003692:Delayed-Action Preparations; D006133:Growth Substances; D007773:Lactates; D011091:Polyesters; D011108:Polymers; D011506:Proteins; D019344:Lactic Acid; C033616:poly(lactide); D002118:Calcium