Increased salt concentration reversibly destabilizes p53 quaternary structure and sequence-specific DNA binding.
Growth suppression by p53 correlates with sequence-specific DNA binding and is determined by tertiary and quaternary protein structures. Exposure to 300 mM NaCl did not affect p53 tertiary structure, but dissociated high-molecular-mass complexes with concomitant loss of specific DNA binding. Both effects were reversible. We conclude that high salt can reversibly destabilize the quaternary structure of p53 that is most efficient for sequence-specific DNA binding.
Butcher
S
S
Department of Biology, University of York, Heslington, U.K.
Hainaut
P
P
Milner
J
J
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002412:Cations; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D007163:Immunosorbent Techniques; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009994:Osmolar Concentration; D010957:Plasmids; D014176:Protein Biosynthesis; D011487:Protein Conformation; D011817:Rabbits; D012965:Sodium Chloride / Q000494:pharmacology*; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D002412:Cations; D016159:Tumor Suppressor Protein p53; D012965:Sodium Chloride; D004247:DNA