Microplate-based DNA hybridization assays for detection of human retroviral gene sequences.
Nonisotopic, microwell-based DNA hybridization assays for the specific detection of human immunodeficiency virus type 1 (HIV-1) gag, human T-cell lymphotropic virus type I (HTLV-I) pol, and HTLV-II pol DNA sequences were evaluated. The performances of these detection kits (Gene Detective enzyme oligonucleotide assays; Cellular Products, Inc., Buffalo, N.Y.) were assessed by using clinical samples whose infection status were established by amplification by PCR and then liquid hybridization detection by using virus-specific probes. Peripheral blood mononuclear cell lysates from 59 HIV-1-, 35 HTLV-I-, and 19 HTLV-II-infected individuals and from 15 healthy blood donors were used as substrates for PCR amplification. The results of the study demonstrated a clinical sensitivity of 100%. In addition, the enzyme oligonucleotide assays were able to detect 1 to 10 proviral copies subsequent to PCR amplification, indicating an analytical sensitivity comparable to that of liquid hybridization.
Dyster
L M
LM
Cellular Products, Inc., Buffalo, New York 14202.
Abbott
L
L
Bryz-Gornia
V
V
Poiesz
B J
BJ
Papsidero
L D
LD
D002460:Cell Line; D004279:DNA, Viral / Q000097:blood / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005814:Genes, Viral; D015750:Genes, gag; D015751:Genes, pol; D015658:HIV Infections / Q000175:diagnosis / Q000382:microbiology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification; D015490:HTLV-I Infections / Q000175:diagnosis / Q000382:microbiology; D015491:HTLV-II Infections / Q000175:diagnosis / Q000382:microbiology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000302:isolation & purification; D015367:Human T-lymphotropic virus 2 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D012190:Retroviridae / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D004279:DNA, Viral