title: Presence of colonization factor antigen IV (CS5CS6) in O29:H21 enterotoxigenic Escherichia coli isolated from children with diarrhea in Brazil.
abstract: 1. Enterotoxigenic Escherichia coli (ETEC) strains belonging to serotype O29:H21 were analyzed for the presence of colonization factor antigens (CFA). 2. CFA/IV was detected in all twelve strains studied, and immunological analysis demonstrated that it was composed of coli surface antigens 5 (CS5) and 6 (CS6) presenting molecular weights of approximately 21 kDa and approximately 16 kDa, respectively. 3. CS6 antigenic homology was observed between O29:H21 and reference strains E11881C and E17018A. 4. CS6 of the O29:H21 strains was composed of two peptide bands, similar to the reference strain E17018A.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, São Paulo, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: Giraldi
authors.forename: B E
authors.initials: BE
authors.lastname: Guth
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification*; D015153:Blotting, Western; D002648:Child; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000276:immunology*; D036781:Fimbriae Proteins; D006386:Hemagglutination Tests; D006801:Humans; D005779:Immunodiffusion; D008970:Molecular Weight
chemical_list: D001426:Bacterial Proteins; C022924:colonization factor antigens; D036781:Fimbriae Proteins