title: Growth factor gene expression in bronchoalveolar lavage cells from patients with lung fibrosis.
abstract: We have studied the gene expression of ten growth factors in bronchoalveolar lavage (BAL) cells from patients with lung fibrosis by using a reverse-transcription-DNA polymerase chain reaction (RT-PCR) technique. IL-1 beta mRNA was detected in almost all of the samples, and TGF beta and IGF-I mRNA were detected in some. The BAL supernatant from the patients was found to have mitogenic activity for lung fibroblasts. Moreover, human recombinant IL-1 beta, TGF beta and IGF-I were found to promote the proliferation of lung fibroblasts. These data suggest that IL-1 beta, TGF beta and IGF-I gene expression in BAL cells might be involved in the development of lung fibrosis.
authors.affiliation: Department of Immunology, Norman Bethune University of Medical Sciences, Changchun.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: G
authors.initials: G
authors.lastname: Yang
mesh_terms: D000328:Adult; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D001999:Bronchoscopy; D015870:Gene Expression; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis*; D017563:Lung Diseases, Interstitial / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*
chemical_list: D007375:Interleukin-1; D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I