title: Synthesis of novel tellurium containing analogues of choline and acetylcholine and their quantitation by pyrolysis-gas chromatography-mass spectrometry.
abstract: Methods for the synthesis and quantitation of the novel choline analogues, telluronium choline and acetyltelluronium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with [2H4]telluronium choline and [2H4]acetyltelluronium choline as internal standards. The telluronium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 232 and m/z 190 for acetyltelluronium and telluronium choline, respectively, and at m/z 236 and m/z 194 for the analogous deuterated internal standards. The assay was linear over a range of 20 pmol-20 nmol of compound taken through the assay.
authors.affiliation: Department of Chemistry and Biochemistry, University of South Carolina, Columbia 29208.
authors.forename: S E
authors.initials: SE
authors.lastname: Harris
authors.forename: L A
authors.initials: LA
authors.lastname: Silks
authors.forename: R B
authors.initials: RB
authors.lastname: Dunlap
authors.forename: J D
authors.initials: JD
authors.lastname: Odom
authors.forename: J W
authors.initials: JW
authors.lastname: Kosh
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009942:Organometallic Compounds / Q000032:analysis / Q000138:chemical synthesis*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013691:Tellurium / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D009942:Organometallic Compounds; C086332:acetyltelluronium choline; C086331:d(0)-telluronium choline; D013691:Tellurium