Identification of minireovirus as a Norwalk-like virus in pediatric patients with gastroenteritis.
In 1977, 30- to 32-nm virus-like particles, named minireovirus because of their unique morphologic appearance, were detected by electron microscopy in the stools of infants and young children with gastroenteritis. Sequence analysis of approximately 2,800 consecutive bases derived from overlapping PCR clones of a recent minireovirus clinical isolate showed 52% nucleotide sequence identity with the Norwalk virus sequence and, in addition, demonstrated that the genomic organizations of these two viruses were similar. Our data show that minireovirus is a Norwalk-like virus and should now also be included in the Caliciviridae family.
Lew
J F
JF
Laboratory of Infectious Disease, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Petric
M
M
Kapikian
A Z
AZ
Jiang
X
X
Estes
M K
MK
Green
K Y
KY
D000595:Amino Acid Sequence; D001483:Base Sequence; D002139:Caliciviridae / Q000145:classification* / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000382:microbiology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000382:microbiology*; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D009663:Norwalk virus / Q000145:classification / Q000235:genetics; D009864:Ontario / Q000453:epidemiology; D017386:Sequence Homology, Amino Acid