Protein synthesis measured in the whole body of chick embryos cultured in vitro.
1. Whole body protein synthesis was measured in chick embryos cultured in vitro. On day 7 of incubation chick embryos were cultured for 60 min in synthetic serum-free medium containing 4-[3H]phenylalanine. Specific radioactivities in free and protein-bound phenylalanine in the whole embryo were measured, starting 2 min after commencement of the culture process. 2. The values for fractional synthesis rate (FSR) estimated in vitro at 20, 30, 45 and 60 min during the embryo culture agreed well, ranging from 35 to 40%/d, suggesting that the method would serve as a useful model for studying the effect of growth promoters in chick embryos. 3. Bovine insulin in the synthetic medium did not affect FSR of protein in chick embryos cultured in vitro.
Kita
K
K
Laboratory of Animal Nutrition, School of Agriculture, Nagoya University, Japan.
Nakagawa
S
S
Pinontoan
R
R
Okumura
J
J
Muramatsu
T
T
D000818:Animals; D002642:Chick Embryo / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D010649:Phenylalanine / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects
D007328:Insulin; D011506:Proteins; D010649:Phenylalanine