Production of granulocyte-macrophage colony stimulating factor by human T-lymphotropic virus type I-infected human glioma cells.
We investigated the production of granulocyte-macrophage colony stimulating factor (GM-CSF) by human T-lymphotropic virus type I (HTLV-I)-infected human glioma cells (KG-1-C and T98G). When glioma cells were co-cultured with HTLV-I-producing T cell lines (HCT-1 and MT-2), GM-CSF was detected in the culture supernatant. GM-CSF was produced in all the co-cultures even after several passages. In co-cultures of KG-1-C and HCT-1 cells with Millicell, the amount of GM-CSF produced in the supernatant was almost as low as in the culture of HCT-1 alone. Moreover, for co-cultures of KG-1-C and HCT-1 or MT-2 cells, the production of GM-CSF was significantly suppressed in the presence of IgG from patients with HAM. Double-label immunostaining showed that GM-CSF-producing glioma cells always were stained by a monoclonal antibody against HTLV-I p19, indicating that HTLV-I infection of glioma cells caused GM-CSF production. These data suggest that human glial cells infected with HTLV-I gain the ability to produce cytokines.
Nishiura
Y
Y
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Nakamura
T
T
Takino
H
H
Ichinose
K
K
Nagasato
K
K
Ohishi
K
K
Tsujihata
M
M
Nagataki
S
S
D000911:Antibodies, Monoclonal / Q000276:immunology; D001932:Brain Neoplasms / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005910:Glioma / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D015490:HTLV-I Infections / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007150:Immunohistochemistry; D013601:T-Lymphocytes / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D016178:Granulocyte-Macrophage Colony-Stimulating Factor