Radioimmunometric quantification of surface lactoferrin in blood mononuclear cells.
A radioimmunometric method was developed for the quantification of lactoferrin molecules natively bound to blood monocyte and lymphocyte surfaces and the estimation of the surface lactoferrin-binding capacity of these cells after their incubation with exogenous lactoferrin. Values of surface lactoferrin obtained were greatest for monocyte-rich isolates (9,168 +/- 1,713 molecules/cell; n = 19). The values of monocyte surface lactoferrin for males were similar to those of premenopausal females (8,980 +/- 2,378 (n = 8) and 9,427 +/- 2,606 molecules/cell (n = 11), respectively), but males had slightly lower values of monocyte surface lactoferrin binding capacity than did premenopausal females (10,447 +/- 2,478 molecules/cell versus 15,958 +/- 3,731 molecules/cell, respectively; p > 0.05). Expressed as saturation of the monocyte surface lactoferrin binding capacity, values of 97.2% +/- 22.6% for males and 76.6% +/- 14.3% for females were calculated. Intermediate values of surface lactoferrin were found in B-lymphocyte-rich isolates from five patients with B-cell chronic lymphocytic leukemia. In T-lymphocyte-rich preparations, there were low levels of native lactoferrin expression (154 +/- 63 molecules of lactoferrin/cell; 3 isolates). The present technique should permit additional quantitative studies of mononuclear cell surface lactoferrin to determine the role of lactoferrin surface binding and analyses of factors that modulate this binding.
Butler
T W
TW
Department of Medicine, University of Alabama at Birmingham.
Heck
L W
LW
Berkow
R
R
Barton
J C
JC
D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000097:blood*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D017697:Premenopause; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D012737:Sex Factors; D013601:T-Lymphocytes / Q000378:metabolism
D007781:Lactoferrin