Occlusive patch method for skin sensitization in guinea pigs: the Buehler method.
Currently, the European Community is in the process of discussing the details of how to conduct various protocols for safety assessment. Additionally, there is a desire that these protocols be harmonized internationally. This presentation attempts to describe the critical and non-critical parameters of the protocol for detecting potential contact allergens in the guinea pig and also discusses some controversial issues. It is also emphasized that the identification of a test material as a potential sensitizer is only the first step in the risk-assessment exercise.
Buehler
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Hill Top Biolabs, Inc., Cincinnati, OH 45242.
D000823:Animal Identification Systems; D000818:Animals; D003877:Dermatitis, Contact; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D006204:Hair Removal; D006968:Hypersensitivity, Delayed / Q000139:chemically induced*; D008297:Male; D010328:Patch Tests / Q000379:methods* / Q000592:standards; D012149:Restraint, Physical