title: The effect of hypercarbia on age-related changes in cerebral glucose transport and glucose-modulated agonal glycolytic rates.
abstract: This study examined the effect of hypercarbia on cerebral agonal glycolytic rates and brain lactate accumulation after complete ischemia induced by cardiac arrest. Before cardiac arrest, the blood plasma glucose concentration in seven newborn (113 d postconception; normal gestation, 115 d) and seven 1-mo-old (144 d postconception) piglets was adjusted to a specific value (range, 1 to 64 mM), and then inspired ventilation gases were changed to 10:50:40 CO2:O2:N2 for 20 min. The agonal glycolytic rate was measured by monitoring the rate of cerebral lactate formation in vivo using proton nuclear magnetic resonance spectroscopy, and postmortem brain lactate concentrations were measured biochemically in tissue extracts obtained 40 to 45 min after cardiac arrest. These data were compared with 21 normocarbic piglets of similar age, nine examined as part of the present study and 12 examined previously (Corbett RJT, Laptook AR, Ruley JI, Garcia D: Pediatr Res 30:579-586, 1991). There was a nonlinear relationship between the final postmortem brain lactate concentration and preischemia blood plasma glucose concentration that was most prominent in newborn piglets and previously had gone unnoticed. When analyzed using a steady-state model for glucose transport, this relationship revealed that normocarbic newborns had a lower preischemia affinity constant for the transport mechanism for glucose (2.8 +/- 1.5 mM) and lower cerebral glucose utilization rate relative to transport rate (0.12 +/- 0.04), compared with 1-mo-olds (4.5 +/- 1.4 mM and 0.30 +/- 0.03, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Ralph Rogers and Mary Nell Magnetic Resonance Center, Department of Radiology, University of Texas Southwestern Medical Center, Dallas 75235-9085.
authors.forename: R J
authors.initials: RJ
authors.lastname: Corbett
authors.forename: A R
authors.initials: AR
authors.lastname: Laptook
authors.forename: R
authors.initials: R
authors.lastname: Sterett
authors.forename: G
authors.initials: G
authors.lastname: Tollefsbol
authors.forename: D
authors.initials: D
authors.lastname: Garcia
mesh_terms: D000140:Acidosis, Lactic / Q000209:etiology / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D001692:Biological Transport; D002545:Brain Ischemia / Q000209:etiology / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006323:Heart Arrest / Q000150:complications; D006935:Hypercapnia / Q000150:complications / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013552:Swine; D013556:Swine, Miniature
chemical_list: D007773:Lactates; D019344:Lactic Acid; D005947:Glucose