Two-dimensional electrophoresis of human salivary proteins from patients with sialoadenopathy.
Unstimulated saliva was fractionated by micro two-dimensional gel electrophoresis, and the proteins visualized by silver staining and immunostaining. The subjects with Sjögren's syndrome exhibited both quantitative and qualitative alterations in the protein composition of the saliva not only from the parotid gland but also from the submandibular/sublingual glands.
Mogi
M
M
Department of Oral Biochemistry, Matsumoto Dental College, Nagano, Japan.
Harada
M
M
Kage
T
T
Chino
T
T
Yoshitake
K
K
D000418:Albumins / Q000032:analysis; D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007781:Lactoferrin / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010309:Parotitis / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012793:Sialadenitis / Q000378:metabolism; D012859:Sjogren's Syndrome / Q000378:metabolism*; D000516:alpha-Amylases / Q000032:analysis
D000418:Albumins; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D012471:Salivary Proteins and Peptides; D000516:alpha-Amylases; D007781:Lactoferrin