title: Unusual structures of the tandem repetitive DNA sequences located at human centromeres.
abstract: The presence of the highly conserved repetitive DNA sequence d(AATGG)n.d(CCATT)n in human centromeres argues for a special role for this sequence in recognition, most probably through the formation of an unusual structure during mitosis. Quantitative one- and two-dimensional nuclear magnetic resonance (1D/2D NMR) spectroscopic studies reveal that the Watson-Crick duplex d(AATGG)n.d(CCATT)n adopts the usual B-DNA conformation as illustrated by taking d(AATGG)3.d(CCATT)3 as an example, whereas the d(CCATT)n strand is essentially a random coil. In contrast, the d(AATGG)n strand adopts an unusual stem-loop motif for repeat lengths n = 2, 3, 4, and 6. In addition to normal Watson-Crick A.T pairs, the stem-loop structures are stabilized by mismatched A.G and G.G pairs in the stem and G-G-A stacking in the loop. Stem-loop structures of d(AATGG)n are independently verified by gel electrophoresis and nuclease digestion studies and were also previously shown to be as stable as the corresponding Watson-Crick duplex d(AATGG)n.d(CCATT)n [Grady et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 1695-1699]. Therefore, the sequence d(AATGG)n can, indeed, nucleate a stem-loop structure at little free energy cost, and if, during mitosis, it is located on the chromosome surface, it can provide specific recognition sites for kinetochore function.
authors.affiliation: Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
authors.forename: P
authors.initials: P
authors.lastname: Catasti
authors.forename: G
authors.initials: G
authors.lastname: Gupta
authors.forename: A E
authors.initials: AE
authors.lastname: Garcia
authors.forename: R
authors.initials: R
authors.lastname: Ratliff
authors.forename: L
authors.initials: L
authors.lastname: Hong
authors.forename: P
authors.initials: P
authors.lastname: Yau
authors.forename: R K
authors.initials: RK
authors.lastname: Moyzis
authors.forename: E M
authors.initials: EM
authors.lastname: Bradbury
mesh_terms: D001483:Base Sequence; D002503:Centromere / Q000648:ultrastructure*; D006801:Humans; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012091:Repetitive Sequences, Nucleic Acid