title: Isolation of an R- M+ mutant of Yersinia enterocolitica serotype O:8 and its application in construction of rough mutants utilizing mini-Tn5 derivatives and lipopolysaccharide-specific phage.
abstract: A generally applicable procedure was used to isolate a spontaneous restriction-deficient mutant of Yersinia enterocolitica serotype O:8. Transposition frequency in the mutant strain 8081-res was approximately 6.7 x 10(-6) per recipient, while it was practically zero in the wild-type strain 8081-c. Mobilization frequency into 8081-res was 10(5) times higher than that into the wild-type strain. The mutant had lost the ability to express the YenI restriction endonuclease activity present in serotype O:8 strains. This allowed the construction of a transposon library in 8081-res. Insertion mutants with transposons in the genes of the rfa region were selected from this library.
authors.affiliation: Department of Medical Microbiology, University of Turku, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Skurnik
mesh_terms: D001435:Bacteriophages; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D008070:Lipopolysaccharides; D009154:Mutation / Q000235:genetics*; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D004251:DNA Transposable Elements; D008070:Lipopolysaccharides