title: Crystal structure of human angiogenin reveals the structural basis for its functional divergence from ribonuclease.
abstract: Angiogenin, a potent inducer of neovascularization, is the only angiogenic molecule known to exhibit ribonucleolytic activity. Its overall structure, as determined at 2.4 A, is similar to that of pancreatic ribonuclease A, but it differs markedly in several distinct areas, particularly the ribonucleolytic active center and the putative receptor binding site, both of which are critically involved in biological function. Most strikingly, the site that is spatially analogous to that for pyrimidine binding in ribonuclease A differs significantly in conformation and is "obstructed" by glutamine-117. Movement of this and adjacent residues may be required for substrate binding to angiogenin and, hence, constitute a key part of its mechanism of action.
authors.affiliation: School of Biology and Biochemistry, University of Bath, Claverton Down, United Kingdom.
authors.forename: K R
authors.initials: KR
authors.lastname: Acharya
authors.forename: R
authors.initials: R
authors.lastname: Shapiro
authors.forename: S C
authors.initials: SC
authors.lastname: Allen
authors.forename: J F
authors.initials: JF
authors.lastname: Riordan
authors.forename: B L
authors.initials: BL
authors.lastname: Vallee
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000648:ultrastructure*; D011994:Recombinant Proteins; D012259:Ribonuclease, Pancreatic; D012260:Ribonucleases / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D011506:Proteins; D011994:Recombinant Proteins; D012260:Ribonucleases; C046366:angiogenin; D012259:Ribonuclease, Pancreatic