Modulation of 12-0-tetradecanoylphorbol-13-acetate-induced epidermal ornithine decarboxylase activity by calcium and verapamil in mouse.
Calcium has been suggested to be an intracellular second messenger for ornithine decarboxylase (ODC) induction caused by 12-O-tetradecanoylphorbol-13-acetate (TPA). In the present study, the effects of dietary calcium supplement and calcium and verapamil injections on TPA-induced ODC activity in skin was investigated in CD-1 and SENCAR mouse. Dietary calcium supplement did not influence ODC induction caused by TPA. However, calcium injections enhanced the TPA-induced ODC activity in CD-1 and SENCAR mouse skin. Verapamil injections resulted in a significant decrease in TPA-induced ODC activity in CD-1 and SENCAR mice. These results suggest that calcium and calcium antagonists may play a role in skin tumor promotion caused by TPA.
Dwivedi
C
C
College of Pharmacy, South Dakota State University, Brookings 57007-0099.
Baer
R K
RK
Jarvis
D M
DM
D000818:Animals; D002136:Calcium, Dietary / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D012867:Skin / Q000187:drug effects / Q000201:enzymology*; D013045:Species Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology*
D002136:Calcium, Dietary; D014700:Verapamil; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate