title: Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms.
abstract: When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.
authors.affiliation: Department of Microbiology, Montana State University, Bozeman 59717.
authors.forename: E D
authors.initials: ED
authors.lastname: Kopczynski
authors.forename: M M
authors.initials: MM
authors.lastname: Bateson
authors.forename: D M
authors.initials: DM
authors.lastname: Ward
mesh_terms: D001483:Base Sequence; D002678:Chimera / Q000235:genetics*; D002999:Clone Cells; D000458:Cyanobacteria / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal