A monoclonal antibody (ER-HR3) against murine macrophages. I. Ontogeny, distribution and enzyme histochemical characterization of ER-HR3-positive cells.
We describe ER-HR3, a monoclonal antibody directed against bone marrow-derived haemopoietic reticulum cells. ER-HR3-positive cells have the electron-microscopic and enzyme-histochemical characteristics of macrophages. Additionally, they are able to phagocytoze. The ER-HR3 antigen is expressed by a majority of blood monocytes and is present on a subpopulation of resident macrophages in multiple organs. ER-HR3-positive cells are abundant in the bone marrow, the splenic red pulp, the mesenteric lymphoid paracortex and the interfollicular areas of the Peyer's patch. Few ER-HR3-positive cells have been observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Moreover, epidermal Langerhans cells express the antigen. No cross-reactivity with other cell types has been found. It is concluded that ER-HR3 has a unique distribution pattern distinct from other macrophage-specific antibodies.
de Jong
J P
JP
Department of Hematology, Erasmus University, Rotterdam, The Netherlands.
Voerman
J S
JS
van der Sluijs-Gelling
A J
AJ
Willemsen
R
R
Ploemacher
R E
RE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000943:Antigens, Differentiation / Q000032:analysis*; D001854:Bone Marrow Cells; D004064:Digestive System / Q000166:cytology; D005314:Embryonic and Fetal Development; D005260:Female; D006412:Hematopoietic Stem Cells / Q000276:immunology; D007668:Kidney / Q000166:cytology; D008221:Lymphoid Tissue / Q000166:cytology; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D010587:Phagocytosis
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D008565:Membrane Proteins