[The bovine zona pellucida: differences in macromolecular composition between oocytes, pre-treated with A-23187 or not, and embryos].
The SDS-PAGE method was used to determine the composition of isolated bovine zona pellucida (ZP). This egg extracellular coat appears to be characterized by 3 major glycoproteins (ZP1, ZP2, ZP3), with apparent molecular weight (MW) of 80-70 kD, 66-63 kD and 60 kD, respectively, as revealed by 1-dimensional SDS-PAGE. After 2-dimensional SDS-PAGE, the zona pellucida electrophoretic pattern indicates a fourth glycoprotein, thus called ZP4. SDS-PAGE analysis of ZP isolated from oocytes and embryos after transit through the genital tract of A23187 pretreated oocytes allowed the description of modifications in glycoproteinic composition.
Bercegeay
S
S
CHU-HME, laboratoire de Biologie de la reproduction, Nantes, France.
Allaire
F
F
Jean
M
M
L'Hermite
A
A
Bruyas
J F
JF
Renard
N
N
Tainturier
D
D
Barrière
P
P
D000818:Animals; D001755:Blastocyst / Q000737:chemistry*; D000001:Calcimycin / Q000494:pharmacology*; D002417:Cattle / Q000196:embryology / Q000378:metabolism*; D004527:Egg Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005326:Fetal Proteins / Q000032:analysis*; D008562:Membrane Glycoproteins / Q000032:analysis*; D008970:Molecular Weight; D009865:Oocytes / Q000737:chemistry* / Q000187:drug effects; D011956:Receptors, Cell Surface; D015044:Zona Pellucida / Q000737:chemistry* / Q000187:drug effects; D000072216:Zona Pellucida Glycoproteins
D004527:Egg Proteins; D005326:Fetal Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; D000001:Calcimycin
La zone pellucide bovine: différences de composition macromoléculaire entre ovocytes, prétraités ou non à l'A 23187, et embryons.