Preparation and use of polyphosphate-modified zirconia for purification of nucleic acids and proteins.
Polyphosphates of chain lengths between 3 and 100 were coupled to porous zirconia particles and used for affinity chromatography of nucleic acids and proteins. It is demonstrated that single-stranded RNA and DNA are strongly retained on the polyphosphate-modified zirconia support, while double-stranded DNA is only weakly bound if at all. Protein separation on this packing can be performed by applying both high-performance liquid chromatography and batch procedures. The results show that polyphosphate-modified zirconia is a useful affinity packing for immobilization and purification of biomacromolecules.
Lorenz
B
B
Institut für Physiologische Chemie, Universität, Mainz, Germany.
Marmé
S
S
Müller
W E
WE
Unger
K
K
Schröder
H C
HC
D000469:Alkaline Phosphatase / Q000302:isolation & purification; D002352:Carrier Proteins / Q000032:analysis; D003850:Deoxyribonuclease I / Q000302:isolation & purification; D009696:Nucleic Acids / Q000302:isolation & purification*; D011122:Polyphosphates / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification*; D012260:Ribonucleases / Q000302:isolation & purification; D015040:Zirconium
D002352:Carrier Proteins; D009696:Nucleic Acids; D011122:Polyphosphates; D011506:Proteins; D015040:Zirconium; D012260:Ribonucleases; D003850:Deoxyribonuclease I; D000469:Alkaline Phosphatase; C028541:zirconium oxide