Reactivity of polyclonal human CD3 antiserum in lymphoid tissues of cattle, sheep, goats, rats and mice.
Polyclonal rabbit antiserum to human T-cell CD3 was used to study its reactivity in lymphoid tissues (lymph nodes, spleen, aggregated lymphoid follicles [Peyer's patches], thymus) of several animal species (cattle, sheep, goats, rats, and mice). Using a peroxidase-antiperoxidase technique on formalin-fixed and paraffin-embedded tissues, immunoreactive cells were detected in T cell-dependent areas of the lymphoid tissues. Reactivity was high in all species tested, but mouse tissues had reduced reactivity, compared with the other species. To obtain a reaction, it was necessary to digest tissues with pronase before application of the immunocytochemical technique. Our results indicate that CD3 antiserum may specifically recognize T-lymphoid cells as it does in human lymphoid tissues and can be used as a marker to study physiologic and pathologic conditions of the lymphoid system of these species.
Ramos-Vara
J A
JA
Departamento de Patología Animal, Facultad de Veterinaria, Universidad Autónoma de Barcelona, Spain.
Miller
M A
MA
López
E
E
Prats
N
N
Brevik
L
L
D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D017252:CD3 Complex / Q000032:analysis* / Q000276:immunology*; D002417:Cattle; D006041:Goats; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology*; D051379:Mice; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
D015703:Antigens, CD; D017252:CD3 Complex; D007106:Immune Sera