Effects of nitric oxide on red blood cells: changes in erythrocyte resistance to hypotonic hemolysis and potassium efflux by experimental maneuvers that decrease nitric oxide.
To determine the role of nitric oxide (NO)-dependent mechanisms on erythrocyte properties, we exposed red cells to L-arginine competitive analogues, 8Br-cyclic guanosine monophosphate (8Br-cGMP) and neutrophil-eliminating filters. These treatments significantly decreased hypotonic hemolysis, increased potassium efflux and caused a spiculate change in erythrocyte morphology. These effects were related to a decrease of NO caused by the three types of treatments.
Caramelo
C
C
Instituto de Investigaciones Médicas, Fundación Jiménez Díaz, Universidad Autónoma, Madrid, Spain.
Riesco
A
A
Outeiriño
J
J
Millás
I
I
Blum
G
G
Monzú
B
B
Casado
V
V
Sánchez
L
L
Mosquera
J R
JR
Casado
S
S
D000328:Adult; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007038:Hypotonic Solutions; D007700:Kinetics; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009504:Neutrophils / Q000502:physiology; D009569:Nitric Oxide / Q000097:blood*; D011188:Potassium / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D019323:omega-N-Methylarginine
D007038:Hypotonic Solutions; D019323:omega-N-Methylarginine; C016276:8-bromocyclic GMP; D009569:Nitric Oxide; D001120:Arginine; D006152:Cyclic GMP; D011188:Potassium; D019331:NG-Nitroarginine Methyl Ester