title: [Optimization of determining human erythrocyte glutathione reductase activity using a semiautomatic analyzer].
abstract: A relatively simple procedure was developed for estimation of the glutathione reductase activity using half-automatic analyzer. The procedure involved: preparation of hemolysates with minimal rate of erythrocyte destruction; substitution of buffers with high ionic strength for 0.05 M Tris-HCl buffer not containing EDTA. Most effective period of preincubation was found; dependence of the reaction rate on FAD was studied. The procedure developed was approved using two types of half-automatic analyzer and the model FP-901 was shown to be the most suitable for estimation of the glutathione reductase holoenzyme activity.
authors.forename: G Iu
authors.initials: GIu
authors.lastname: Mal'tsev
authors.forename: L A
authors.initials: LA
authors.lastname: Orlova
mesh_terms: D001331:Automation; D002021:Buffers; D004912:Erythrocytes / Q000201:enzymology*; D005980:Glutathione Reductase / Q000097:blood*; D006801:Humans; D013053:Spectrophotometry / Q000295:instrumentation
chemical_list: D002021:Buffers; D005980:Glutathione Reductase
vernacular_title: Optimizatsiia opredeleniia aktivnosti glutationreduktazy éritrotsitov cheloveka na poluavtomaticheskom analizatore.