Comparative study on the effects of chlorite oxygen reaction product TCDO (tetrachlorodecaoxygen) and sodium chlorite solution (NaClO2) with equimolar chlorite content on bone marrow and peripheral blood of BDIX rats.
The effects of the chlorite-oxygen reaction product TCDO (tetrachlorodecaoxygen, active ingredient of the systemic application form of WF 10) were investigated on bone marrow and peripheral blood of BDIX rats in comparison to a sodium chlorite solution with a chlorite content identical to that of WF 10. Despite difficulties in determining the chemical differences between TCDO and a sodium chlorite solution, their differing effects on cells, tissue and organism were striking. The following characteristics have been observed: Stimulation of the bone marrow, evidenced by the pronounced increase in mature granulocytes, pronormo- and normoblasts, or increased cell proliferation rate, determined by means of the BrdUrd method, was achieved only with WF 10 (TCDO). Stimulation of the bone marrow led in turn to increased numbers of leucocytes and monocytes in the peripheral blood. In addition, WF 10 induced the production of large granular lymphocytes (LGLs), referred to as natural killer cells (NK-cells). In contrast, NaClO2 solution suppressed bone marrow function, exhibiting a toxic effect when given on a long-term basis. At the same time the number of mature granulocytes as well as pronormo- and normoblasts decreased, while the presence of LGLs was not observed. The results showed that TCDO is a potent stimulator of the bone marrow function and an effective modulator of the entire immune system. The toxic effect of chlorite, derived from the TCDO matrix, is not noticeable, being completely compensated by the favourable effects of TCDO.
Kempf
S R
SR
German Cancer Research Centre, Institute for Toxicology and Chemotherapy, Heidelberg.
Blaszkiewitz
K
K
Reim
M
M
Ivankovic
S
S
D000818:Animals; D001769:Blood / Q000187:drug effects*; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D002713:Chlorine / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010087:Oxides / Q000494:pharmacology*; D051381:Rats; D013154:Spleen / Q000187:drug effects
D002712:Chlorides; D010087:Oxides; D002713:Chlorine; C041955:tetrachlorodecaoxide; C001599:chlorite