Neutrophil activation by monomeric interleukin-8.
Interleukin-8 (IL-8), a pro-inflammatory protein, has been shown by nuclear magnetic resonance (NMR) and x-ray techniques to exist as a homodimer. An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectivity block formation of hydrogen bonds between monomers and thereby prevent dimerization. This analog was shown to be a monomer, as assessed by analytical ultracentrifugation and NMR. Nevertheless, it was equivalent to IL-8 in assays of neutrophil activation, which indicates that the monomer is a functional form of IL-8.
Rajarathnam
K
K
Protein Engineering Network of Centres of Excellence (PENCE), University of Alberta, Edmonton, Canada.
Sykes
B D
BD
Kay
C M
CM
Dewald
B
B
Geiser
T
T
Baggiolini
M
M
Clark-Lewis
I
I
D002118:Calcium / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D006801:Humans; D006860:Hydrogen Bonding; D016209:Interleukin-8 / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D019272:Leukocyte Elastase; D008956:Models, Chemical; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010196:Pancreatic Elastase / Q000378:metabolism; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D018123:Receptors, Interleukin / Q000737:chemistry / Q000378:metabolism; D023062:Receptors, Interleukin-8A
D016209:Interleukin-8; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D002118:Calcium