title: Inactivation of the streptokinase gene prevents Streptococcus equisimilis H46A from acquiring cell-associated plasmin activity in the presence of plasminogen.
abstract: The streptokinase gene of Streptococcus equisimilis H46 was inactivated by plasmid insertion mutagenesis to study the relationship between elaboration of streptokinase and acquisition of cell-associated plasmin activity after incubation of wild-type and mutant cells in media containing plasminogen or plasmin. The results showed that H46A binds both the zymogen and active enzyme, generates surface-associated plasmin activity in the presence of plasminogen when producing streptokinase, and expresses its plasmin(ogen) receptor(s) independently of a functional streptokinase gene. At least part of the plasmin(ogen) binding capacity may be due to the glyceraldehyde-3-phosphate dehydrogenase type of receptor molecule, as judged by the detection of the corresponding gene.
authors.affiliation: Institute for Molecular Biology, Jena University, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Malke
authors.forename: U
authors.initials: U
authors.lastname: Mechold
authors.forename: K
authors.initials: K
authors.lastname: Gase
authors.forename: D
authors.initials: D
authors.lastname: Gerlach
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial; D005341:Fibrinolysin / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010958:Plasminogen / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D055293:Receptors, Urokinase Plasminogen Activator; D015183:Restriction Mapping; D013290:Streptococcal Infections / Q000382:microbiology; D013291:Streptococcus / Q000201:enzymology* / Q000235:genetics; D013300:Streptokinase / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D010958:Plasminogen; D013300:Streptokinase; D005341:Fibrinolysin