title: A quantitative method for analysis of radiolabelled proteoglycans synthesized by cultured human arterial smooth muscle cells.
abstract: 1. Sulphate labelled proteoglycans (PG) synthesized by cultured human arterial smooth muscle cell have been quantified using an improved method based on a combination of specific enzymes and ethanol precipitation. 2. The present method gives quantitative data of PGs and subclasses allowing batchwise analysis of a large number of samples. 3. Approximately 81% +/- 1.7% (mean +/- SD, n = 6) of total PGs synthesized by human arterial smooth muscle cells accumulated in medium. 4. In cell layer and medium chondroitin sulphate proteoglycan constituted 65.0% +/- 0.3% and 75.8% +/- 0.7% (mean +/- SD, n = 3), respectively of sulphated PGs. 5. Heparan sulphate proteoglycan accounted for 26.8% +/- 0.6% in cell layer and 22.6% +/- 0.5% (mean +/- SD, n = 3) in medium of sulphated PGs.
authors.affiliation: Research Laboratory of Biochemical Pathology, Aarhus Kommunehospital, University of Aarhus, Denmark.
authors.forename: V B
authors.initials: VB
authors.lastname: Thøgersen
authors.forename: L
authors.initials: L
authors.lastname: Heickendorff
authors.forename: T
authors.initials: T
authors.lastname: Ledet
mesh_terms: D001011:Aorta; D002478:Cells, Cultured; D011232:Chemical Precipitation; D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange; D000431:Ethanol; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D013462:Sulfur Radioisotopes
chemical_list: D011509:Proteoglycans; D013462:Sulfur Radioisotopes; D000431:Ethanol