Analysis of the gonadal sex of five intersex pigs using Y chromosomal markers.
DNA isolated from gonadal tissue of five intersex pigs was analyzed for the presence of the Y chromosomal markers ZFY and DYZ1. Four cases showed no detectable male-specific DNA-sequences. The fifth case exhibited a weak male-specific band at Southern blot analysis with the human ZFY probe and showed a male-specific PCR-product with DYZ1 primers, indicating an XX/XY gonadal chimera.
Thomsen
P D
PD
Division of Anatomy, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Poulsen
P H
PH
D000818:Animals; D001483:Base Sequence; D002678:Chimera / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D012734:Disorders of Sex Development / Q000235:genetics / Q000662:veterinary*; D005260:Female; D005819:Genetic Markers; D006066:Gonads / Q000648:ultrastructure; D008297:Male; D008969:Molecular Sequence Data; D013552:Swine; D013553:Swine Diseases / Q000235:genetics*; D014998:Y Chromosome
D015342:DNA Probes; D005819:Genetic Markers