NMR spectroscopic investigation of agarose oligomers produced by an alpha-agarase.
The 13C NMR signals of various even and odd agarose oligosaccharides with either D-galactose or 3,6-anhydro-alpha-L-galactose at the reducing end have been assigned. The chemical shifts in water of the agaro- and the neoagaro-oligosaccharides are compared and the influence of dimethyl sulfoxide on the chemical structure of the agaro-oligosaccharides is reported. The 3,6-anhydro-L-galactose residue at the reducing end of agaro-oligosaccharides is in the hydrated form.
Rochas
C
C
Centre de Recherches sur le Macromolecules Végétales, CNRS-UPR 5301, Grenoble, France.
Potin
P
P
Kloareg
B
B
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002250:Carbon Radioisotopes; D006026:Glycoside Hydrolases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D012685:Sepharose / Q000737:chemistry* / Q000378:metabolism; D013055:Spectrophotometry, Infrared
D002250:Carbon Radioisotopes; D009844:Oligosaccharides; D012685:Sepharose; D006026:Glycoside Hydrolases; C019624:agarase