Effect of magnesium sulphate infusion on ex vivo platelet aggregation in swine.
The effect of magnesium sulphate infusion on ex vivo platelet aggregation in 11 female Yorkshire swine was observed using platelet-rich plasma and different agonists (ADP 5 mM; ADP 10 mM and collagen 1 mg/ml). Infusion of 1 g MgSO4 over 1 h produced a significant decrease in platelet aggregability. A dose-dependent effect of different ADP concentrations on platelet aggregation was noticed. Platelet inhibition was most consistent when using ADP 5 mM. We estimate this concentration of agonist as optimal in swine. The swine model is a good choice for investigation of in vivo platelet activation, especially with regard to cardiovascular research. We conclude that there is an inhibitory effect of supplemental magnesium on ex vivo platelet aggregation in swine with initial normomagnesaemia.
Herzog
W R
WR
University of Maryland Medical Center, Department of Medicine, Baltimore.
Atar
D
D
Gurbel
P A
PA
Vogel
R A
RA
Schlossberg
M L
ML
Serebruany
V L
VL
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D003094:Collagen / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D007262:Infusions, Intravenous; D008278:Magnesium Sulfate / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013552:Swine
D010975:Platelet Aggregation Inhibitors; D000244:Adenosine Diphosphate; D008278:Magnesium Sulfate; D003094:Collagen