Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes.
We report here the DNA sequence of a segment (alpha 1006.13: YBLO5) of chromosome II of Saccharomyces cerevisiae, extending over 32.5 kb. The segment contains 26 open reading frames (ORFs) from YBLO501 to YBLO526. YBL0505 corresponds to the SEC17 gene and YBL0521 to the KIP1 gene. YBL0516 contains an intron, YBL0513 shows homology with the RAT protein phosphatase and YBL0526 contains a zinc-finger motif. Disruption of 14 genes by insertion of a URA3 cassette has been performed and these mutants were analysed for their mating and sporulation ability, and for their growth on different carbon sources. YBL0515 and YBL0526 ORFs seem to be involved in the sporulation process.
Scherens
B
B
Institut de Recherches du CERIA/COOVI, Laboratoire de Microbiologie, Université Libre de Bruxelles, Belgium.
el Bakkoury
M
M
Vierendeels
F
F
Dubois
E
E
Messenguy
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins; D005800:Genes, Fungal; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D016335:Zinc Fingers / Q000235:genetics
D004271:DNA, Fungal; D005656:Fungal Proteins; C431747:KIP1 protein, S cerevisiae; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D029701:Saccharomyces cerevisiae Proteins; D010749:Phosphoprotein Phosphatases