Spermidine acetylation in N1 and N8 position in rat brain and in N-ethyl-N-nitrosourea-induced gliomas.
N-ethyl-N-nitrosourea-induced rat gliomas showed a stimulation of cytosolic spermidine N-acetyltransferase activity compared with normal brain, with an increased formation of N1 and N8-acetylspermidine, suggesting the activation of two enzymes acetylating the polyamine in N1 and N8 position, respectively. The enhancement in cytosolic spermidine N1-acetyltransferase was probably responsible for an activation of the polyamine interconversion pathway in gliomas, as indicated by an accumulation of N1-acetylspermidine and a marked increase in putrescine. Spermidine N8-acetyltransferase, although acetylating histones at a high rate, seemed distinct from the nuclear enzyme, as, unlike from the latter, it showed very low affinity for putrescine and was not inhibited by methylglyoxal bis(guanylhydrazone).
Sessa
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Centro di Studio sulla Patologia Cellulare, Consiglio Nazionale delle Ricerche, Milano, Italy.
Broglia
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Terreni
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MR
Perin
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D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D001932:Brain Neoplasms / Q000139:chemically induced / Q000201:enzymology* / Q000378:metabolism; D002384:Catalysis; D003600:Cytosol / Q000201:enzymology; D005038:Ethylnitrosourea; D005260:Female; D005910:Glioma / Q000139:chemically induced / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism*; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D005038:Ethylnitrosourea; D013095:Spermidine; D000099228:Diamine N-Acetyltransferase; C030223:N(8)-acetylspermidine; C017988:N(1)-acetylspermidine