title: Analysis of derivatized peptides by capillary electrophoresis.
abstract: Thirteen synthetic prothrombin leader peptides differing only in C terminus were analyzed by capillary electrophoresis. These peptides were derivatized using the novel, fluorescent derivatizing agent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. Derivatized peptides were detected using fluorescence and ultraviolet absorption signals, and underivatized peptides were detected using ultraviolet absorbance at 185 nm. Data are presented which compare the analysis of the derivatized and underivatized peptides.
authors.affiliation: University of Rhode Island, Kingstown 02881.
authors.forename: K M
authors.initials: KM
authors.lastname: De Antonis
authors.forename: P R
authors.initials: PR
authors.lastname: Brown
authors.forename: Y F
authors.initials: YF
authors.lastname: Cheng
authors.forename: S A
authors.initials: SA
authors.lastname: Cohen
mesh_terms: D000595:Amino Acid Sequence; D000634:Aminoquinolines; D002219:Carbamates; D004586:Electrophoresis / Q000379:methods*; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D011516:Prothrombin / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: C078178:6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; D000634:Aminoquinolines; D002219:Carbamates; D005456:Fluorescent Dyes; D010455:Peptides; D011516:Prothrombin