Decorin and a large dermatan sulfate proteoglycan in bovine striated muscle.
Proteoglycans were extracted and isolated from adult bovine muscle tissue by dissociative extraction followed by density gradient centrifugation, gel chromatography and ion-exchange chromatography. Two proteoglycans were characterized; one of large molecular size (PG-L) and one of small molecular size (PG-S). The recovery of PG-L and PG-S was 33% and 67%, respectively. By cellulose acetate electrophoresis before and after treatment with chondroitinase AC and ABC both samples were shown to carry predominantly dermatan sulfate chains. The large proteoglycan was recognized with an antibody against a large dermatan sulfate proteoglycan from bovine sclera, whereas the small was recognized by an antibody against decorin from bovine sclera. Chondroitinase ABC treatment of PG-S followed by SDS-PAGE showed a core protein with a molecular weight of 45 kDa, which also reacted with the decorin antibody. Amino-acid analysis of both PG-L and PG-S revealed an amino-acid composition closely similar, although not identical, to the large dermatan sulfate proteoglycan from bovine sclera and decorin, respectively. Immunohistochemical analyses of muscle tissue sections showed that decorin and the large dermatan sulfate proteoglycan are present in the perimysium layers of muscle tissue, although although with a somewhat different pattern of distribution. Decorin was, in addition, found in the endomysium.
Eggen
K H
KH
Norwegian Food Research Institute, As.
Malmstrøm
A
A
Kolset
S O
SO
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D058575:Decorin; D003871:Dermatan Sulfate / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D005629:Frozen Sections; D007150:Immunohistochemistry; D008297:Male; D009132:Muscles / Q000378:metabolism*; D011509:Proteoglycans / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
D000596:Amino Acids; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D003871:Dermatan Sulfate