Incorporation of phospholipid analogues into the plasma membrane affects ATP-induced vesiculation of human erythrocyte ghosts.
The influence of various spin-labelled phospholipid analogues on the ATP-dependent vesiculation of human erythrocyte ghosts at 37 degrees C was investigated by monitoring the acetylcholinesterase activity. After incorporation of analogues into the outer leaflet a decline of endocytic vesiculation was observed. However, suppression was arrested after about 20 min when ghosts have been labelled with a phosphatidylserine analogue known to redistribute rapidly to the inner leaflet. These data suggest that vesiculation of erythrocyte ghosts is affected by differential expansion of the inner and outer leaflet.
Müller
P
P
Humboldt-Universität zu Berlin, Germany.
Pomorski
T
T
Herrmann
A
A
D000110:Acetylcholinesterase / Q000097:blood; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D004705:Endocytosis / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008563:Membrane Lipids / Q000097:blood*; D010743:Phospholipids / Q000097:blood*; D013113:Spin Labels; D013329:Structure-Activity Relationship; D013997:Time Factors
D008563:Membrane Lipids; D010743:Phospholipids; D013113:Spin Labels; D000255:Adenosine Triphosphate; D000110:Acetylcholinesterase