Erythrocyte behavior in free-flow electrophoresis is independent of erythrocyte age.
Enzyme activities (acetylcholinesterase, fluorescein diacetate hydrolase, superoxide dismutase), membrane protein band 4.1a/4.1b ratio and density of various fractions of rabbit erythrocytes separated did not show differences between various fractions of erythrocytes separated by free-flow electrophoresis. Also, electric field deflection of lightest and densest fractions of erythrocytes did not differ. These results point to the lack of changes in surface charge density during in vivo erythrocyte aging.
Bartosz
G
G
Department of Molecular Biophysics, University of Lódź, Poland.
Guclu
J
J
Soudain
P
P
D000110:Acetylcholinesterase / Q000097:blood; D000818:Animals; D002452:Cell Count; D002469:Cell Separation; D003598:Cytoskeletal Proteins; D004586:Electrophoresis; D004905:Erythrocyte Aging / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D005452:Fluoresceins; D006867:Hydrolases / Q000097:blood; D008565:Membrane Proteins / Q000097:blood; D009479:Neuropeptides; D011817:Rabbits; D013482:Superoxide Dismutase / Q000097:blood
D003598:Cytoskeletal Proteins; D005452:Fluoresceins; D008565:Membrane Proteins; D009479:Neuropeptides; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1; D013482:Superoxide Dismutase; D006867:Hydrolases; D000110:Acetylcholinesterase; C018506:diacetylfluorescein