title: Is the coiled body involved in nucleolar functions?
abstract: Coiled bodies (CBs) are structural constituents observed in nuclei of most eukaryotic cells. They usually occur in the nucleoplasm as well as in contact with the nucleolar surface. In this work we studied the hepatocyte nuclei of hibernating dormice in order to investigate possible modifications of CBs along the seasonal cycle. CBs were abundant during hibernation and rapidly disappeared upon arousal from hibernation. Moreover, CBs were frequently found to be integrated into the nucleolar body. Immunocytochemical analyses showed that CBs contain nucleoplasmic as well as nucleolar RNA-processing factors, suggesting an "ambiguous" role for this organelle in the nuclear functions.
authors.affiliation: Center of Electron Microscopy, University of Lausanne, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Malatesta
authors.forename: C
authors.initials: C
authors.lastname: Zancanaro
authors.forename: T E
authors.initials: TE
authors.lastname: Martin
authors.forename: E K
authors.initials: EK
authors.lastname: Chan
authors.forename: F
authors.initials: F
authors.lastname: Amalric
authors.forename: R
authors.initials: R
authors.lastname: Lührmann
authors.forename: P
authors.initials: P
authors.lastname: Vogel
authors.forename: S
authors.initials: S
authors.lastname: Fakan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D006605:Hibernation; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D009687:Nuclear Proteins / Q000276:immunology / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism; D012377:Rodentia
chemical_list: D000911:Antibodies, Monoclonal; D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D012322:RNA Precursors; D017411:Ribonucleoproteins, Small Nuclear; C047458:fibrillarin; C068921:p80-coilin