Biochemical properties of attachment region binding protein ARBP.
ARBP (attachment region binding protein) is an abundant nuclear protein that specifically binds to matrix/scaffold attachment regions (MARs/SARs). Here we show by gel filtration and gradient sedimentation that ARBP has an elongated shape. The sedimentation coefficient was determined as only 2.1 S. Furthermore, limited proteolysis of ARBP in situ (in isolated nuclei) with several proteases generated limiting resistant peptides from 14.5 to 18 kDa, that retained the ability to bind MARs specifically. This indicates that these peptides encompass the DNA binding domain of ARBP.
von Kries
J P
JP
Institut für Physiologische Chemie, Universitäts-Krankenhaus Eppendorf, Hamburg, Germany.
Rosorius
O
O
Buhrmester
H
H
Strätling
W H
WH
D000818:Animals; D002499:Centrifugation, Density Gradient; D002645:Chickens; D002843:Chromatin / Q000737:chemistry; D002850:Chromatography, Gel; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005260:Female; D015394:Molecular Structure; D009687:Nuclear Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D011487:Protein Conformation
D002843:Chromatin; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments