A mass spectrometric solution to the address problem of combinatorial libraries.
The molecular weights of femtomole quantities of small peptides attached to polystyrene beads have been determined with imaging time-of-flight secondary ion mass spectrometry. The analysis is made possible by the selective clipping of the bond linking the peptide to a bead with trifluoroacetic acid vapor before the secondary ion mass spectrometry assay. The approach can be applied to large numbers of 30- to 60-micrometer polystyrene beads for the direct characterization of massive combinatorial libraries.
Brummel
C L
CL
Department of Chemistry, Pennsylvania State University, University Park 16802.
Lee
I N
IN
Zhou
Y
Y
Benkovic
S J
SJ
Winograd
N
N
D000595:Amino Acid Sequence; D007091:Image Processing, Computer-Assisted; D013058:Mass Spectrometry / Q000379:methods*; D008863:Microspheres; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry*; D011137:Polystyrenes
D009842:Oligopeptides; D011137:Polystyrenes