Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013.
New crystals of a blue copper protein, pseudoazurin from denitrifier Achromobacter cycloclastes IAM1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4 degrees C. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) A. The asymmetric unit includes one molecule of pseudoazurin with a Vm value of 2.04 A3/Da. The crystals are so stable against X-ray irradiation that a complete data set up to 1.54 A has been collected using a single native crystal. Solution of the structure was performed by means of the Patterson search techniques, and the current crystallographic R-factor is 17.5% at 3.0 A resolution. Refinement at higher resolution is in progress.
Inoue
T
T
Department of Applied Chemistry, Faculty of Engineering, Osaka University.
Nishio
N
N
Kai
Y
Y
Harada
S
S
Ohshiro
Y
Y
Suzuki
S
S
Kohzuma
T
T
Shidara
S
S
Iwasaki
H
H
D000421:Alcaligenes / Q000737:chemistry*; D001400:Azurin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D018360:Crystallography, X-Ray
C047163:pseudoazurin; D001400:Azurin