Three-dimensional crystal structure of recombinant erabutoxin a at 2.0 A resolution.
Recombinant erabutoxin a (Ea(r)) has been crystallized by vapour diffusion in hanging drops. The crystals belong to space group P2(1)2(1)2(1) with cell dimensions a = 55.8 A, b = 53.4 A, c = 40.8 A. Diffraction data have been recorded on a FAST detector up to 2.0 A. The atomic crystal structure of Ea(r) has been determined by initial refinement of the structure of the isotoxin erabutoxin b (Eb) the crystals of which were grown under identical conditions. The R-factor was 23% at 2.0 A resolution. The secondary and tertiary structures of Ea(r) are shown to be identical with that of wild-type Eb, within the experimental error.
Arnoux
B
B
Laboratoire de Biologie Structurale, CNRS, Gif-sur-Yvette, France.
Ménez
R
R
Drevet
P
P
Boulain
J C
JC
Ducruix
A
A
Ménez
A
A
D003460:Crystallization; D018360:Crystallography, X-Ray; D004870:Erabutoxins / Q000737:chemistry*; D009498:Neurotoxins / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry
D009498:Neurotoxins; D011994:Recombinant Proteins; C038908:erabutoxin A; D004870:Erabutoxins