Induction of a common pathway of apoptosis by staurosporine.
The present observations show that staurosporine can rapidly trigger both the morphological changes and intranucleosomal DNA fragmentation typical of apoptosis. This occurred in a number of cell lines from various origins regardless of the state of differentiation and cell cycle phase, suggesting the presence of a common inducible suicide pathway. The broad apoptotic activity of staurosporine appears to be unique among other protein kinase or phosphatase inhibitors we tested. Results obtained in a cell-free assay suggest that cytoplasmic proteins directly modulated by staurosporine may be involved in a ubiquitous signal for the induction of DNA fragmentation and apoptosis.
Bertrand
R
R
Centre de Recherche Louis-Charles Simard, Institut du Cancer de Montréal, Québec, Canada.
Solary
E
E
O'Connor
P
P
Kohn
K W
KW
Pommier
Y
Y
D000470:Alkaloids / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002474:Cell-Free System; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D047428:Protein Kinase Inhibitors; D019311:Staurosporine
D000470:Alkaloids; D047428:Protein Kinase Inhibitors; D004247:DNA; D010744:Phosphoric Monoester Hydrolases; D019311:Staurosporine