A serological survey for bovine immunodeficiency-like virus in Ontario dairy cattle and associations between test results, production records and management practices.
A chemiluminescence Western blot analysis (WBA) for detecting antibovine immunodeficiency-like virus (BIV) antibodies, had good repeatability. The test was subsequently applied to a bank of serum samples from 928 adult cows from 265 herds in Ontario; the number of cows sampled within each herd ranged from 1 to 13. The overall prevalence of anti-BIV antibodies among cows was 5.5% with a 95% confidence interval of 4.2% to 7.2%. In contrast, 18.1% of herds had at least one reactor among cows tested, resulting in a herd-prevalence confidence interval of 13.8% to 23.4%. These estimates of prevalence were in the same range as previous reports from the US and Europe. Bovine immunodeficiency-like virus may have a worldwide distribution. Unfortunately, BIV test sensitivity and specificity are difficult to estimate because virus isolation is inefficient. Therefore, the apparent prevalences could not be adjusted for test sensitivity and specificity, to estimate the true prevalence of infection. The serum samples had previously been tested for antibodies to bovine leukemia virus (BLV). There were no significant associations between BIV and BLV test results. Least squares regression was used to investigate potential associations between BIV test results and selected production indices.(ABSTRACT TRUNCATED AT 250 WORDS)
McNab
W B
WB
Animal and Plant Health Directorate, Agriculture Canada, Nepean, Ontario.
Jacobs
R M
RM
Smith
H E
HE
D000822:Animal Husbandry; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015153:Blotting, Western / Q000379:methods; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology*; D005260:Female; D016087:Immunodeficiency Virus, Bovine / Q000302:isolation & purification*; D007774:Lactation; D016180:Lentivirus Infections / Q000453:epidemiology / Q000662:veterinary*; D008163:Luminescent Measurements; D009864:Ontario / Q000453:epidemiology; D015995:Prevalence
D000914:Antibodies, Viral