A novel human homologue of a dead-box RNA helicase family.
Putative cDNA clones for a nuclear antigen that cross-reacts with anti-human aldolase A monoclonal antibody MAb1A2 were isolated from the HeLa lambda gt11 cDNA library and a candidate clone (clone 3) was analyzed. The cDNA has an open reading frame (ORF) of 1,317 bp encoding a novel RNA helicase belonging to the DEAD RNA helicase family. The ORF also contains a nuclear targeting signal and the epitope for MAb1A2. The putative RNA helicase has sequence similarity to Escherichia coli RNA helicase DEAD, mouse translation factor eIF-4A, and human p68 and p54.
Kitajima
Y
Y
Department of Biochemistry, Saga Medical School, Japan.
Yatsuki
H
H
Zhang
R
R
Matsuhashi
S
S
Hori
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D039601:Eukaryotic Initiation Factor-4A; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010448:Peptide Initiation Factors / Q000235:genetics; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000096:biosynthesis* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010448:Peptide Initiation Factors; D039601:Eukaryotic Initiation Factor-4A; D012316:RNA Nucleotidyltransferases; D020365:RNA Helicases; D005634:Fructose-Bisphosphate Aldolase