Intraperitoneal injection of synthetic bacterial lipopeptides does not cause a rise in circulating inflammatory cytokines.
The production of TNF-alpha, IL-1 and IL-6 was measured in mice after i.p. injections of the synthetic bacterial lipopeptide Pam3Cys-Ala-Gly, a potent macrophage and B cell activator in vitro. Only minor amounts of IL-6 and no TNF-alpha were detectable in the serum of mice injected with 10 or 100 micrograms of Pam3Cys-Ala-Gly. Lipopeptide concentrations up to 1000 micrograms failed to induce IL-1, and TNF-alpha production and serum IL-6 levels were only slightly elevated. In contrast to Pam3Cys-Ala-Gly, i.p. injections of LPS were accompanied by high levels of the inflammatory cytokines TNF-alpha, IL-1 and IL-6 suggesting fundamental differences of the mode of action of these two substances when applied in vivo.
Hauschildt
S
S
Institute of Immunobiology, University of Freiburg, FRG.
Beuscher
H U
HU
Jung
G
G
Bessler
W
W
Ulmer
A
A
D000595:Amino Acid Sequence; D000818:Animals; D007274:Injections, Intraperitoneal; D007375:Interleukin-1 / Q000096:biosynthesis / Q000097:blood*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008074:Lipoproteins / Q000008:administration & dosage / Q000138:chemical synthesis / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000096:biosynthesis
D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008074:Lipoproteins; D014409:Tumor Necrosis Factor-alpha; C061110:N-palmitoyl-5,6-dipalmitoylcysteinyl-alanyl-glycine