Analysis of derivatized peptides by capillary electrophoresis.
Thirteen synthetic prothrombin leader peptides differing only in C terminus were analyzed by capillary electrophoresis. These peptides were derivatized using the novel, fluorescent derivatizing agent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. Derivatized peptides were detected using fluorescence and ultraviolet absorption signals, and underivatized peptides were detected using ultraviolet absorbance at 185 nm. Data are presented which compare the analysis of the derivatized and underivatized peptides.
De Antonis
K M
KM
University of Rhode Island, Kingstown 02881.
Brown
P R
PR
Cheng
Y F
YF
Cohen
S A
SA
D000595:Amino Acid Sequence; D000634:Aminoquinolines; D002219:Carbamates; D004586:Electrophoresis / Q000379:methods*; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D011516:Prothrombin / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
C078178:6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; D000634:Aminoquinolines; D002219:Carbamates; D005456:Fluorescent Dyes; D010455:Peptides; D011516:Prothrombin