Comparative study between molecular hybridization and electron microscopy for the detection of hepatitis E virus.
The study describes a comparison between molecular hybridization using a non-radiolabeled, thymine-thymine (TT) dimerized synthetic oligonucleotide complementary DNA probe and electron microscopy for the detection of hepatitis E virus genome in bile. Spot hybridization with the TT dimerized probe was found to be more sensitive and specific compared to electron microscopy.
Soe
S
S
Department of Medical Research No. 5, Yangon, Myanmar.
Nakane
P K
PK
Myat
P K
PK
Iida
F
F
Kyi
K P
KP
Swe
T
T
D000818:Animals; D001646:Bile / Q000382:microbiology*; D018076:DNA, Complementary; D004279:DNA, Viral; D016679:Genome, Viral; D016751:Hepatitis E / Q000382:microbiology*; D016752:Hepatitis E virus / Q000235:genetics / Q000302:isolation & purification*; D008854:Microscopy, Electron / Q000379:methods*; D008992:Monkey Diseases / Q000382:microbiology*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes; D011740:Pyrimidine Dimers; D012680:Sensitivity and Specificity; D013997:Time Factors
D018076:DNA, Complementary; D004279:DNA, Viral; D015345:Oligonucleotide Probes; D011740:Pyrimidine Dimers