title: Fluorescent in situ hybridization for the detection of t(8:14) in Burkitt's lymphoma.
abstract: Burkitt's lymphoma cells exhibit the reciprocal balanced chromosomal translocation t(8:14) in 75% of patients. Cytogenetic analysis is time-consuming, requires in vitro culture with mitogens and enables to analyse a relatively small number of cells. We evaluated the role of multicolor fluorescent in situ hybridization (FISH) in the rapid detection of t(8:14) in Burkitt's lymphoma cells. FISH detected the JH/myc translocation in 100% of the cells of five cell lines carrying the classical t(8:14) and in fresh cells obtained from a newly diagnosed Burkitt's lymphoma patient. In contrast, the translocation was not detected in the Bajb cell line that does not carry t(8:14). We conclude that FISH is a rapid and reliable diagnostic tool for the detection of the JH/myc translocation in Burkitt's lymphoma patients.
authors.affiliation: Department of Medicine, Meir Hospital, Kfar Saba, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Lishner
authors.forename: G
authors.initials: G
authors.lastname: Kenet
authors.forename: A
authors.initials: A
authors.lastname: Lalkin
authors.forename: S
authors.initials: S
authors.lastname: Yarkoni
authors.forename: H
authors.initials: H
authors.lastname: Ben-Bassat
authors.forename: M
authors.initials: M
authors.lastname: Fejgin
authors.forename: G
authors.initials: G
authors.lastname: Rechavi
authors.forename: A
authors.initials: A
authors.lastname: Amiel
mesh_terms: D001707:Biopsy, Needle; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000473:pathology; D002648:Child; D002883:Chromosomes, Human, Pair 14; D002898:Chromosomes, Human, Pair 8; D005069:Evaluation Studies as Topic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured