title: A second promoter and enhancer element within the immunoglobulin heavy chain locus.
abstract: The joining of immunoglobulin gene segments during B cell development consists of a tightly regulated series of rearrangement steps. A variety of experiments have suggested that transcription is involved in activating the locus as substrate for the V(D)J recombinase. Here, we have characterized a region located immediately upstream of the most J-proximal D element (DQ52), which contains both promoter and enhancer activities preferentially active in precursors of B cells. Interestingly, this DQ52 regulatory element is inevitably deleted in fully rearranged H chain genes. We propose that it is involved in the early activation and rearrangement events at the IgH locus.
authors.affiliation: Max-Planck-Institut für Immunbiologie, Freiburg, FRG.
authors.forename: A H
authors.initials: AH
authors.lastname: Kottmann
authors.forename: B
authors.initials: B
authors.lastname: Zevnik
authors.forename: M
authors.initials: M
authors.lastname: Welte
authors.forename: P J
authors.initials: PJ
authors.lastname: Nielsen
authors.forename: G
authors.initials: G
authors.lastname: Köhler
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D004742:Enhancer Elements, Genetic; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
chemical_list: D007143:Immunoglobulin Heavy Chains