title: Encoded latent membrane protein 1 of Epstein-Barr virus on follicular dendritic cells in residual germinal centres in Hodgkin's disease.
abstract: OBJECTIVE
  To determine if there is an association between Epstein-Barr virus (EBV) infection and Hodgkin's disease.
  
  
  METHODS
  Fifty cases of Hodgkin's disease and 25 reactive lymph nodes were screened for the presence of EBV-RNA (EBER) using in situ hybridisation, and for the expression of EBV encoded latent membrane protein 1 (LMP-1) by immunohistochemistry.
  
  
  RESULTS
  In 42% of the cases of Hodgkin's disease, EBER was detected in the nuclei of the malignant cells, and in LMP-1 expression was found 36%. Both EBER and LMP-1 positivity were seen in 34% of the cases. An additional finding was the presence of LMP-1 on follicular dendritic cells in residual germinal centres in two cases of Hodgkin's disease. EBER was not detected in these germinal centres. In reactive lymph nodes only occasional EBER positive, small, lymphoid cells were found, without LMP-1 expression.
  
  
  CONCLUSIONS
  These results show a strong correlation between the presence of EBER and the LMP-1 expression in the Reed-Sternberg cells. They corroborate a role for EBV in at least some cases of Hodgkin's disease. LMP-1 is probably presented as an immune complex in the germinal centres, as part of an immune response against EBV.
authors.affiliation: Department of Pathology, University Hospital, Utrecht, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: van Gorp
authors.forename: K C
authors.initials: KC
authors.lastname: Jacobse
authors.forename: R
authors.initials: R
authors.lastname: Broekhuizen
authors.forename: J
authors.initials: J
authors.lastname: Alers
authors.forename: J G
authors.initials: JG
authors.lastname: van den Tweel
authors.forename: R A
authors.initials: RA
authors.lastname: de Weger
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D003713:Dendritic Cells / Q000737:chemistry* / Q000276:immunology; D006566:Herpesviridae Infections / Q000150:complications; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D017404:In Situ Hybridization, Fluorescence; D008198:Lymph Nodes / Q000382:microbiology; D012367:RNA, Viral / Q000032:analysis; D016539:Reed-Sternberg Cells / Q000276:immunology / Q000382:microbiology; D014412:Tumor Virus Infections / Q000150:complications; D014759:Viral Envelope Proteins / Q000032:analysis; D014763:Viral Matrix Proteins / Q000032:analysis*
chemical_list: D000956:Antigens, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins