Lipid peroxidation in hepatic microsomal membranes isolated from mice in health and in experimental leishmaniasis.
Normal hepatic microsomal membranes when exposed in vitro to different free radicals, cause membrane damage by lipid peroxidation which could be monitored by the analysis of malonaldehyde formation and measurement of membrane microviscosity. Lipid peroxidation in vivo, when examined in hepatic microsomal membranes in experimental Leishmaniasis, reveals a direct relationship between membrane microviscosity and the extent of lipid peroxidation. Scavengers of free radicals and peroxides such as superoxide dismutase (SOD) for O2.-, mannitol for (OH.) and catalase for H2O2 in modest amounts were used for preventing the membrane damage caused by lipid peroxidation.
Bagchi
M
M
Biomembrane Division, Indian Institute of Chemical Biology, Calcutta.
Mukherjee
S
S
Basu
M K
MK
D000818:Animals; D016166:Free Radical Scavengers; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007893:Leishmania donovani; D007898:Leishmaniasis, Visceral / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology; D012016:Reference Values
D016166:Free Radical Scavengers; D016877:Oxidants; D008315:Malondialdehyde