In vitro effects of live and killed Brucella abortus on bovine cytokine and prostaglandin E2 production.
Live and gamma-irradiated-killed Brucella abortus strain 2308 increased interleukin 1 (IL-1), but not interleukin 2 (IL-2), interferon-gamma (IFN-gamma), or prostaglandin E2 (PGE2) production when incubated with normal bovine peripheral blood mononuclear cells (PBMC). Live B. abortus was more effective than killed B. abortus in stimulating IL-1 production by normal PBMC. Both live and killed B. abortus were equally effective in suppressing IL-2 and IFN-gamma production by Concanavalin A-stimulated PBMC. Incubation of PBMC with the cyclo-oxygenase inhibitor, indomethacin, blocked PGE2 synthesis, but did not further enhance IL-1 production or prevent suppressed IL-2 and IFN-gamma production that was induced by live and killed B. abortus. These results suggest that B. abortus-induced suppression of IL-2 and IFN-gamma production did not appear to be mediated by the suppressive prostaglandin, PGE2, or other cyclo-oxygenase metabolites.
Stevens
M G
MG
National Animal Disease Center, United States Department of Agriculture, Agriculture Research Service, Ames, IA 50010.
Olsen
S C
SC
D000818:Animals; D002003:Brucella abortus / Q000276:immunology* / Q000528:radiation effects; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D007213:Indomethacin / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology
D016207:Cytokines; D008070:Lipopolysaccharides; D015232:Dinoprostone; D007213:Indomethacin