Contrasting uptakes of 59Fe into spleen, liver, kidney and some other soft tissues in normal and hypotransferrinaemic mice. Influence of an antibody against the transferrin receptor.
Uptake of iron-59 from blood into various soft tissues of anaesthetized mice was investigated by continuous intravenous infusion of the radiotracer during 2 hr. The 59Fe was given either as ferrous chloride with ascorbate or as 59Fe-transferrin. Infusions were made into adult mice with and without pretreatment with a monoclonal antibody against transferrin receptors, and into hypotransferrinaemic mice and appropriate controls. In normal mice, 59Fe uptake into spleen was much higher than into other tissues and was 94-96% inhibited by the antibody. Inhibitions due to the antibody were less complete in liver and renal cortex, and there was evidence of some non-transferrin-mediated transport during infusion of 59Fe/ascorbate. In the hypotransferrinaemic mice, tissue uptakes of 59Fe during infusion of 59Fe/ascorbate were enormous, being two to three orders of magnitude greater than in the normal controls. The rank order for size of uptake was liver > renal cortex > pancreas > spleen > other tissues. All tissues examined have a considerable potential capacity for uptake of non-transferrin-bound iron, this being greatest in liver and renal cortex.
Bradbury
M W
MW
Biomedical Sciences Division (Physiology), King's College London, U.K.
Raja
K
K
Ueda
F
F
D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D007501:Iron / Q000493:pharmacokinetics*; D007504:Iron Radioisotopes; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D011990:Receptors, Transferrin / Q000276:immunology; D013154:Spleen / Q000378:metabolism*; D014018:Tissue Distribution; D014168:Transferrin / Q000172:deficiency / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007504:Iron Radioisotopes; D011990:Receptors, Transferrin; D014168:Transferrin; D007501:Iron