Multiple cis-acting targeting sequences are required for orb mRNA localization during Drosophila oogenesis.
The targeting of positional information to specific regions of the oocyte or early embryo is one of the key processes in establishing anterior-posterior and dorsal-ventral polarity. In many developmental systems, this is accomplished by localization of mRNAs. The germ line-specific Drosophila orb gene plays a critical role in defining both axes of the developing oocyte, and its mRNA is localized in a complex pattern during oogenesis. We have identified a 280-bp sequence from the orb 3' untranslated region capable of reproducing this complex localization pattern. Furthermore, we have found that multiple cis-acting elements appear to be required for proper targeting of orb mRNA.
Lantz
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Department of Biology, Washington University, St. Louis, Missouri 63130.
Schedl
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D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis; D010053:Ovary / Q000502:physiology; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D001616:beta-Galactosidase / Q000096:biosynthesis
D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D001616:beta-Galactosidase