Relationship between protein adsorptive capacity and the X-ray diffraction pattern of aluminium hydroxide adjuvants.
Thermal treatment during the preparation of aluminium hydroxide adjuvants affects the primary crystallite size of the adjuvant. The primary crystallite size can be characterized by the line broadening of the (020) reflection of the X-ray diffraction pattern. Studies of protein adsorption using bovine serum albumin as a model protein revealed a direct relationship between the albumin adsorptive capacity and the width at half height (WHH) of the (020) reflection in the X-ray diffraction pattern.
Masood
H
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Department of Industrial and Physical Pharmacy, Purdue University, West Lafayette, IN 47907.
White
J L
JL
Hem
S L
SL
D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000327:Adsorption; D000536:Aluminum Hydroxide / Q000737:chemistry*; D000537:Aluminum Oxide / Q000737:chemistry; D000818:Animals; D000941:Antigens / Q000737:chemistry; D002417:Cattle; D018360:Crystallography, X-Ray; D006358:Hot Temperature; D066298:In Vitro Techniques; D011506:Proteins / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology
D000276:Adjuvants, Immunologic; D000941:Antigens; D011506:Proteins; D012710:Serum Albumin, Bovine; D000536:Aluminum Hydroxide; C069471:aluminum oxide hydroxide; D000537:Aluminum Oxide