Affinity chromatographic purification of tetrodotoxin by use of tetrodotoxin-binding high molecular weight substances in the body fluid of shore crab (Hemigrapsus sanguineus) as ligands.
A purification method for tetrodotoxin (TTX), based on affinity chromatography using the TTX-binding high mol. wt substances in the body fluid of shore crab (Hemigrapsus sanguineus) as ligands, was developed. This method was particularly useful for analysis of TTX in biological samples with low concentrations of TTX. The affinity gel prepared was highly specific for TTX, having no ability to bind 4-epi-TTX and anhydro-TTX as well as saxitoxin.
Shiomi
K
K
Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Yamaguchi
S
S
Shimakura
K
K
Nagashima
Y
Y
Yamamori
K
K
Matsui
T
T
D000645:Ammonium Sulfate; D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D003386:Brachyura / Q000737:chemistry*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D008024:Ligands; D008099:Liver / Q000737:chemistry; D008970:Molecular Weight; D013779:Tetrodotoxin / Q000302:isolation & purification*
D008024:Ligands; D013779:Tetrodotoxin; D000645:Ammonium Sulfate