title: Islet cell antigen 512 is a diabetes-specific islet autoantigen related to protein tyrosine phosphatases.
abstract: Islet cell Ag 512 (ICA512) is a recombinant human Ag that was isolated from an islet cDNA expression library by screening with human insulin-dependent diabetes mellitus sera. Specificity of reaction with diabetic sera was demonstrated initially by immunoprecipitation with a small number of diabetic and normal serum samples. To permit quantitative and rapid serum testing, ICA512 was purified and adapted to an ELISA format. In this way, a sensitivity of 48% with newly diagnosed diabetic sera has been measured with a panel of 80 sera. DNA sequencing of ICA512-3, a cDNA that contains a 1644 bp open reading frame, suggests that it codes for a transmembrane protein having a single membrane-spanning segment and a cytoplasmic domain that is closely related to the first intracellular (catalytic) domain of the T cell protein tyrosine phosphatase, CD45. Northern blot analysis of poly(A)+ RNAs from several human tissues indicates that ICA512 mRNA is expressed in brain and pancreas.
authors.affiliation: Institute for Metabolic Disorders, Miles Research Center, West Haven, CT 06516.
authors.forename: D U
authors.initials: DU
authors.lastname: Rabin
authors.forename: S M
authors.initials: SM
authors.lastname: Pleasic
authors.forename: J A
authors.initials: JA
authors.lastname: Shapiro
authors.forename: H
authors.initials: H
authors.lastname: Yoo-Warren
authors.forename: J
authors.initials: J
authors.lastname: Oles
authors.forename: J M
authors.initials: JM
authors.lastname: Hicks
authors.forename: D E
authors.initials: DE
authors.lastname: Goldstein
authors.forename: P M
authors.initials: PM
authors.lastname: Rae
mesh_terms: D000595:Amino Acid Sequence; D001324:Autoantigens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D003920:Diabetes Mellitus / Q000276:immunology*; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology*; D008969:Molecular Sequence Data; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D001324:Autoantigens; D012333:RNA, Messenger; D017027:Protein Tyrosine Phosphatases