title: Effects of nitric oxide on red blood cells: changes in erythrocyte resistance to hypotonic hemolysis and potassium efflux by experimental maneuvers that decrease nitric oxide.
abstract: To determine the role of nitric oxide (NO)-dependent mechanisms on erythrocyte properties, we exposed red cells to L-arginine competitive analogues, 8Br-cyclic guanosine monophosphate (8Br-cGMP) and neutrophil-eliminating filters. These treatments significantly decreased hypotonic hemolysis, increased potassium efflux and caused a spiculate change in erythrocyte morphology. These effects were related to a decrease of NO caused by the three types of treatments.
authors.affiliation: Instituto de Investigaciones Médicas, Fundación Jiménez Díaz, Universidad Autónoma, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Caramelo
authors.forename: A
authors.initials: A
authors.lastname: Riesco
authors.forename: J
authors.initials: J
authors.lastname: Outeiriño
authors.forename: I
authors.initials: I
authors.lastname: Millás
authors.forename: G
authors.initials: G
authors.lastname: Blum
authors.forename: B
authors.initials: B
authors.lastname: Monzú
authors.forename: V
authors.initials: V
authors.lastname: Casado
authors.forename: L
authors.initials: L
authors.lastname: Sánchez
authors.forename: J R
authors.initials: JR
authors.lastname: Mosquera
authors.forename: S
authors.initials: S
authors.lastname: Casado
mesh_terms: D000328:Adult; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007038:Hypotonic Solutions; D007700:Kinetics; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009504:Neutrophils / Q000502:physiology; D009569:Nitric Oxide / Q000097:blood*; D011188:Potassium / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D019323:omega-N-Methylarginine
chemical_list: D007038:Hypotonic Solutions; D019323:omega-N-Methylarginine; C016276:8-bromocyclic GMP; D009569:Nitric Oxide; D001120:Arginine; D006152:Cyclic GMP; D011188:Potassium; D019331:NG-Nitroarginine Methyl Ester