title: Evidence for phage-mediated gene transfer among Pseudomonas aeruginosa strains on the phylloplane.
abstract: As the use of genetically engineered microorganisms for agricultural tasks becomes more frequent, the ability of bacteria to exchange genetic material in the agricultural setting must be assessed. Transduction (bacterial virus-mediated horizontal gene transfer) is a potentially important mechanism of gene transfer in natural environments. This study investigated the potential of plant leaves to act as surfaces on which transduction can take place among microorganisms. Pseudomonas aeruginosa and its generalized transducing bacteriophage F116 were used as a model system. The application of P. aeruginosa lysogens of F116 to plant leaves resulted in genetic exchange among donor and recipient organisms resident on the same plant. Transduction was also observed when these bacterial strains were inoculated onto adjacent plants and contact was made possible through high-density planting.
authors.affiliation: Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater 74078.
authors.forename: S P
authors.initials: SP
authors.lastname: Kidambi
authors.forename: S
authors.initials: S
authors.lastname: Ripp
authors.forename: R V
authors.initials: RV
authors.lastname: Miller
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D008954:Models, Biological; D010944:Plants / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D014162:Transfection / Q000235:genetics*