Cloning of the Escherichia coli radC gene: identification of the RadC protein.
1. The DNA sequence of the radC gene suggests an open reading frame of 297-bp. 2. To identify the gene product, radC was subcloned in an expression vector, pKK223-3 and the RadC protein identified by the maxicell method as a polypeptide of approximately 11 kDa.
Felzenszwalb
I
I
Departamento de Bifísica e Biometria, Universidade do Estado do Rio de Janeiro, Brasil.
Boiteux
S
S
Laval
J
J
D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D009152:Mutagenicity Tests; D010957:Plasmids / Q000235:genetics
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C074495:yicR protein, E coli