title: Susceptibility of human-mouse T cell hybrids to HIV-productive infection.
abstract: Interspecies human x mouse cell hybrids were used to investigate the genetic basis of human permissivity to HTLV-IIIB infection. T cell hybrids between the mouse BW 51.47 T lymphoma line and normal, PHA-IL-2 activated, human peripheral mononuclear cells (PBMCs) were generated. These hybrids preferentially segregated human chromosomes, as assessed by phenotype and karyotype analysis. Viral integration occurred only in those hybrids expressing CD4+ at the cell surface. However, infectious progeny production was demonstrated only in two of the three CD4+ hybrids tested. By segregation analysis, we could correlate the absence of human chromosomes 1, 3, and 9 with the lack of infectious viral progeny.
authors.affiliation: Istituto di Immunologia e Malattie Infettive, Università di Verona, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Ramarli
authors.forename: C
authors.initials: C
authors.lastname: Cambiaggi
authors.forename: C
authors.initials: C
authors.lastname: De Giuli Morghen
authors.forename: P
authors.initials: P
authors.lastname: Tripputi
authors.forename: R
authors.initials: R
authors.lastname: Ortolani
authors.forename: M
authors.initials: M
authors.lastname: Bolzanelli
authors.forename: G
authors.initials: G
authors.lastname: Tridente
authors.forename: R S
authors.initials: RS
authors.lastname: Accolla
mesh_terms: D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000378:metabolism; D002877:Chromosomes, Human; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015750:Genes, gag; D015658:HIV Infections / Q000235:genetics* / Q000382:microbiology; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D051379:Mice; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D016662:Virus Integration / Q000235:genetics
chemical_list: D015704:CD4 Antigens; D017931:DNA Primers; D004279:DNA, Viral