The major dog pancreas protein recognized by an antiserum to dog prostate kallikrein is the anionic trypsin.
1. On the basis of its immunoreactivity with a polyclonal antiserum to dog prostate kallikrein in Western blot experiments, a 30 kDa protein was purified from the pancreas of the dog using ion-exchange and gel filtration chromatography. 2. That protein was identified as the anionic trypsin by its NH2-terminal amino acid sequence. 3. The immunoreaction occurred despite an overall amino acid homology which was limited to 39% between the prostate kallikrein and anionic trypsin. 4. Otherwise, the anti-prostatic kallikrein antiserum was rather specific since it did not react with dog cationic trypsin, dog renal kallikrein and human prostate specific antigen.
Chapdelaine
P
P
Laboratoire de Biorégulation hormonale, Le Centre Hospitalier de l'Université Laval, Sainte-Foy, Québec, Canada.
Lazure
C
C
Deperthes
D
D
Tremblay
R R
RR
Dube
J Y
JY
D000595:Amino Acid Sequence; D000818:Animals; D000838:Anions; D002265:Carboxylic Ester Hydrolases / Q000276:immunology; D004285:Dogs; D007106:Immune Sera; D007610:Kallikreins / Q000032:analysis*; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D011467:Prostate / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D014357:Trypsin / Q000032:analysis*
D000838:Anions; D007106:Immune Sera; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D007610:Kallikreins; D014357:Trypsin