title: Design and synthesis of multi-haem proteins.
abstract: A water-soluble, 62-residue, di-alpha-helical peptide has been synthesized which accommodates two bis-histidyl haem groups. The peptide assembles into a four-helix dimer with 2-fold symmetry and four parallel haems that closely resemble native haems in their spectral and electrochemical properties, including haem-haem redox interaction. This protein is an essential intermediate in the synthesis of molecular 'maquettes', a novel class of simplified versions of the metalloproteins involved in redox catalysis and in energy conversion in respiratory and photosynthetic electron transfer.
authors.affiliation: Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104.
authors.forename: D E
authors.initials: DE
authors.lastname: Robertson
authors.forename: R S
authors.initials: RS
authors.lastname: Farid
authors.forename: C C
authors.initials: CC
authors.lastname: Moser
authors.forename: J L
authors.initials: JL
authors.lastname: Urbauer
authors.forename: S E
authors.initials: SE
authors.lastname: Mulholland
authors.forename: R
authors.initials: R
authors.lastname: Pidikiti
authors.forename: J D
authors.initials: JD
authors.lastname: Lear
authors.forename: A J
authors.initials: AJ
authors.lastname: Wand
authors.forename: W F
authors.initials: WF
authors.lastname: DeGrado
authors.forename: P L
authors.initials: PL
authors.lastname: Dutton
mesh_terms: D000595:Amino Acid Sequence; D015195:Drug Design; D004563:Electrochemistry; D014450:Electron Transport Complex III / Q000138:chemical synthesis / Q000737:chemistry; D006420:Hemeproteins / Q000138:chemical synthesis* / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013057:Spectrum Analysis
chemical_list: D006420:Hemeproteins; D014450:Electron Transport Complex III