APP expression in primary neuronal cell cultures from P6 mice during in vitro differentiation.
Primary neuronal cell cultures from P6 mice were investigated in order to study amyloid protein precursor (APP) gene expression in differentiating neurons. Cerebellar granule cells which strongly express APP 695 allowed the identification of three distinct isoforms of neuronal APP 695. The high-molecular-weight form of APP 695 is sialylated. The expression pattern of neuronal APP 695 changes during in vitro differentiation. Sialylated forms become more abundant upon longer cultivation time. The secreted forms of sialylated, neuronal APP 695 are shown to comigrate with APP isolated from cerebrospinal fluid. We suggest that the different sialylation states of APP 695 may reflect the modulation of cell-cell and cell-substrate interactions during in vitro differentiation and regeneration.
Dichgans
M
M
Center for Molecular Biology, University of Heidelberg, FRG.
Mönning
U
U
König
G
G
Sandbrink
R
R
Masters
C L
CL
Beyreuther
K
K
D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006868:Hydrolysis; D007150:Immunohistochemistry; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012794:Sialic Acids / Q000378:metabolism
D016564:Amyloid beta-Protein Precursor; D012794:Sialic Acids; D008715:Methionine; D009439:Neuraminidase