Capsaicin-like effect of resiniferatoxin in the rat stomach.
Neurochemical and functional studies were performed to investigate and to compare the effects of resiniferatoxin and capsaicin in the rat stomach. Neonatal administration of resiniferatoxin (0.6-1.6 mumol/kg subcutaneously (s.c.)) produced a marked decrease in gastric calcitonin gene-related peptide-like immunoreactivity in both secretory and non-secretory region of the stomach. Almost complete depletion of the peptide was determined by neonatal administration of capsaicin (164 mumol/kg s.c.). Vasoactive intestinal polypeptide-like immunoreactivity was concomitantly unaffected by resiniferatoxin or capsaicin, thus showing the selectivity of action of the neurotoxins on gastric afferent fibers. Oral administration of an equimolar dose (0.3 nmol/kg) of resiniferatoxin or capsaicin together with 50% ethanol reduced at a similar extent gastric haemorrhagic lesions produced by the mucosal barrier-breaker agent. These findings provide evidence that resiniferatoxin and capsaicin may act on a common neuronal target in the rat stomach and that the acute exciting (protective) effect is of the same magnitude.
Tramontana
M
M
Pharmacology Department, Malesci Pharmaceuticals, Firenze, Italy.
Renzi
D
D
Panerai
C
C
Surrenti
C
C
Nappi
F
F
Abelli
L
L
Evangelista
S
S
D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis*; D002211:Capsaicin / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D004224:Diterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity; D005753:Gastric Mucosa / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D006471:Gastrointestinal Hemorrhage / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control; D007279:Injections, Subcutaneous; D008297:Male; D009475:Neurons, Afferent / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D009498:Neurotoxins / Q000494:pharmacology / Q000633:toxicity; D051381:Rats; D013276:Stomach Ulcer / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control; D014660:Vasoactive Intestinal Peptide / Q000096:biosynthesis
D004224:Diterpenes; D009498:Neurotoxins; D014660:Vasoactive Intestinal Peptide; D000431:Ethanol; C024353:resiniferatoxin; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin