Identification of vertebrate volatiles stimulating olfactory receptors on tarsus I of the tick Amblyomma variegatum Fabricius (Ixodidae). I. Receptors within the Haller's organ capsule.
Gas chromatography-coupled electrophysiological recordings (GC-EL) from olfactory sensilla within the capsule of Haller's organ of the tick Amblyomma variegatum indicate the presence of a number of stimulants in rabbit and bovine odours, and in steer skin wash. Some of these stimulants were fully identified by gas chromatography-mass spectrometry analysis and by matching electrophysiological activity of synthetic analogues as: 1) hexanal, 2-heptenal, nonanal, furfural, benzaldehyde, and 2-hydroxybenzaldehyde (in all extracts); 2) heptanal, 2-, 3-, and 4-methylbenzaldehyde, and gamma-valerolactone (only in bovine and rabbit odour). Careful examination of the electrophysiological responses permit characterization of 6 receptor types: 1) a benzaldehyde receptor, 2) a 2-hydroxybenzaldehyde receptor, 3) three types of receptors responding differently to aliphatic aldehydes, and 4) a lactone receptor.
Steullet
P
P
Institute of Zoology, University of Neuchâtel, Switzerland.
Guerin
P M
PM
D000328:Adult; D000818:Animals; D002417:Cattle; D004594:Electrophysiology; D005121:Extremities / Q000294:innervation / Q000502:physiology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D009812:Odorants / Q000032:analysis; D011817:Rabbits; D018035:Receptors, Odorant / Q000502:physiology*; D012679:Sense Organs / Q000502:physiology*; D013987:Ticks / Q000502:physiology*; D014714:Vertebrates / Q000502:physiology*
D018035:Receptors, Odorant