Studies on effects of culture conditions and age of donor on hippocampal neurons in vitro.
The hippocampus is a brain structure of pivotal role in many physiological and pathological responses including memory formation, epilepsy and ischemia. Understanding of these processes requires a good knowledge of hippocampal neurons. In vitro culture offers a tool to study these cells in a controlled environment. In order to optimize culture conditions several growth parameters were investigated in hippocampal neuronal cultures derived from rats of different age: 18-day old fetuses, newborns and 5-day old rat pups, maintained in either serum-containing or chemically defined media. Usefulness of particular culture conditions for special purposes is discussed.
Figiel
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Tissue Culture Unit, Nencki Institute of Experimental Biology, Warsaw, Poland.
Poniński
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Lukasiuk
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Kaczmarek
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D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000502:physiology / Q000648:ultrastructure; D002478:Cells, Cultured; D005260:Female; D006624:Hippocampus / Q000166:cytology* / Q000196:embryology; D007150:Immunohistochemistry; D009457:Neuroglia / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar