The nonapeptide leucinostatin A acts as a weak ionophore and as an immunosuppressant on T lymphocytes.
Earlier studies have disclosed that leucinostatin A, a hydrophobic nonapeptide antibiotic, assumes an alpha-helical secondary structure in nonpolar environments. The present report demonstrates that the peptide acts as a weak ionophore facilitating the transport of mono-and divalent cations through the plasma membrane of T lymphocytes and through artificial membranes. Leucinostatin A does not change the thymidine uptake of both resting mouse thymocytes and peripheral blood lymphocytes but dose-dependently prevents the activation of T lymphocytes by tetradecanoyl-phorbol-acetate and by anti-T cell receptor antibody.
Csermely
P
P
Institute of Biochemistry I, Semmelweis University, School of Medicine, Budapest, Hungary.
Radics
L
L
Rossi
C
C
Szamel
M
M
Ricci
M
M
Mihály
K
K
Somogyi
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism; D002414:Cations, Monovalent / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides; D013601:T-Lymphocytes / Q000187:drug effects*; D015032:Zinc / Q000378:metabolism
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D002413:Cations, Divalent; D002414:Cations, Monovalent; D007166:Immunosuppressive Agents; D007476:Ionophores; D010455:Peptides; C008268:leucinostatin A; D015032:Zinc; D002118:Calcium