Differences between the sugar moieties of liver- and bone-type alkaline phosphatases: a re-evaluation.
We re-evaluated the differences between the sugar moieties of liver and bone alkaline phosphatases (ALPs). Sialic acid was added to ALP sugar moieties by alpha 2,3- or 2,6-sialyltransferase treatment of the asialo-form ALP (neuraminidase-treated ALP). Asialo-bone ALP was converted to a liver-like ALP by the 2,6-sialyltransferase treatment. The resulting liver-like ALP was less susceptible to neuraminidase than non-treated bone ALP, but was still labile to heat exposure at 56 degrees C like non-treated bone ALP. However, after the O-linked sugar moiety had been released by additional treatment with O-glycanase the liver-like ALP became more heat stable at 56 degrees C, like non-treated liver ALP. Non-treated liver ALP reacted specifically with anti-liver ALP monoclonal antibody, and non-treated bone ALP reacted with both anti-liver and anti-bone ALP antibodies. The asialo-bone ALP still reacted with anti-bone ALP antibody, whereas the asialo-form liver ALP showed little, if any, reaction with anti-liver and anti-bone ALP antibodies. Neuraminidase and O-glycanase-treated bone ALP reacted less with anti-bone ALP antibody. After O-glycanase treatment, bone ALP molecules deprived of an O-linked sugar moiety had a molecular size and heat stability similar to liver ALP. The difference between liver and bone ALP molecules may be due not only to their manner of sialic acid linkage but also to the attachment of the O-linked sugar moiety.
Miura
M
M
First Department of Biochemistry, Saitama Medical School, Japan.
Sakagishi
Y
Y
Hata
K
K
Komoda
T
T
D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001842:Bone and Bones / Q000201:enzymology*; D002846:Chromatography, Affinity; D004587:Electrophoresis, Agar Gel; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D037102:Lectins; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000378:metabolism; D037121:Plant Lectins; D012794:Sialic Acids / Q000737:chemistry; D012799:Sialyltransferases / Q000378:metabolism; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D037102:Lectins; D037121:Plant Lectins; D012794:Sialic Acids; C043696:jacalin; D012799:Sialyltransferases; D000469:Alkaline Phosphatase; D006596:Hexosaminidases; D009439:Neuraminidase; C060638:glycopeptide alpha-N-acetylgalactosaminidase; D019158:N-Acetylneuraminic Acid; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase