How to isolate cardiac myocytes.
A complete technique is described for the isolation of myocytes from mammalian hearts using the Langendorff perfusion technique. The use of calcium-free solution containing collagenase and protease, followed by low calcium solution, consistently results in a large number of calcium tolerant myocytes which are well suited for long periods of electrophysiological recording.
Tytgat
J
J
University of Leuven, Laboratory of Physiology, Belgium.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005260:Female; D006168:Guinea Pigs; D006325:Heart Atria / Q000166:cytology*; D006352:Heart Ventricles / Q000166:cytology*; D008297:Male; D011690:Purkinje Fibers / Q000166:cytology*; D011817:Rabbits; D051381:Rats