title: Enzymatic activities correlate with chimaeric substitutions at the actin-binding face of myosin.
abstract: Myosins are a functionally divergent group of mechanochemical enzymes involved in various motile activities in cells. Despite a high degree of conservation in the amino-acid sequence of the 130K motor domain (head region) of the molecule, there are large differences in the enzymatic and motile activities (Tables 1 and 2) of myosins from diverse species and cell types. However, the degree of conservation is not uniform throughout the head sequence; therefore, one reasonable hypothesis is that the functional differences between myosins derive from the poorly conserved areas. The most prominent divergent region occurs at the 50K/20K junction, a region of the molecule sensitive to proteolytic digestion and a binding site for actin. We have now constructed chimaeras of this region of myosin by substituting the 9-amino-acid Dictyostelium junction region with those from myosins from other species and find that the actin-activated ATPase correlates well with the activity of the myosin from which the junction region was derived. Our results suggest that this region, likely to be part of the myosin head that interacts directly with actin, is important in determining the enzymatic activity of myosin.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, California 94305.
authors.forename: T Q
authors.initials: TQ
authors.lastname: Uyeda
authors.forename: K M
authors.initials: KM
authors.lastname: Ruppel
authors.forename: J A
authors.initials: JA
authors.lastname: Spudich
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002645:Chickens; D017931:DNA Primers; D004023:Dictyostelium; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D017931:DNA Primers; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D009218:Myosins