Analysis of some factors involved in the virulence mechanism of Vibrio cholerae non-01.
A study was carried out to evaluate the potential intestinal toxicity of 188 samples of Vibrio cholerae non-01 isolated from seawater found along the beaches of Rio de Janeiro city. Three different assays were carried out involving: (a) detection of vascular permeability factor (PF) in guinea pigs (together with assessment of two culture media for production of the toxin); (b) intestinal fluid accumulation (FA) in suckling mice; and (c) detection of haemolysin. The results demonstrated that both culture media gave a similar level of performance. In the animal assays, 43% of the samples induced PF in guinea pigs, 28.7% caused intestinal fluid accumulation in suckling mice, and 63.28% contained haemolysin. Only 4.25% of the samples gave positive results in all three tests.
Rodrigues
D P
DP
Fundação Oswaldo Cruz, Departamento de Bacteriologio-IOC, Rio de Janeiro, Brazil.
Ribeiro
R V
RV
Alves
R M
RM
Hofer
E
E
D000818:Animals; D016228:Endothelial Growth Factors / Q000032:analysis; D005260:Female; D006168:Guinea Pigs; D006460:Hemolysin Proteins / Q000032:analysis; D007413:Intestinal Mucosa / Q000378:metabolism; D008222:Lymphokines / Q000032:analysis; D008297:Male; D051379:Mice; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014734:Vibrio cholerae / Q000472:pathogenicity*; D014774:Virulence
D016228:Endothelial Growth Factors; D006460:Hemolysin Proteins; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors