Fluorescent in situ hybridization for the detection of t(8:14) in Burkitt's lymphoma.
Burkitt's lymphoma cells exhibit the reciprocal balanced chromosomal translocation t(8:14) in 75% of patients. Cytogenetic analysis is time-consuming, requires in vitro culture with mitogens and enables to analyse a relatively small number of cells. We evaluated the role of multicolor fluorescent in situ hybridization (FISH) in the rapid detection of t(8:14) in Burkitt's lymphoma cells. FISH detected the JH/myc translocation in 100% of the cells of five cell lines carrying the classical t(8:14) and in fresh cells obtained from a newly diagnosed Burkitt's lymphoma patient. In contrast, the translocation was not detected in the Bajb cell line that does not carry t(8:14). We conclude that FISH is a rapid and reliable diagnostic tool for the detection of the JH/myc translocation in Burkitt's lymphoma patients.
Lishner
M
M
Department of Medicine, Meir Hospital, Kfar Saba, Israel.
Kenet
G
G
Lalkin
A
A
Yarkoni
S
S
Ben-Bassat
H
H
Fejgin
M
M
Rechavi
G
G
Amiel
A
A
D001707:Biopsy, Needle; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000473:pathology; D002648:Child; D002883:Chromosomes, Human, Pair 14; D002898:Chromosomes, Human, Pair 8; D005069:Evaluation Studies as Topic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured