In vitro studies on the DNA impairments induced by Cr(III) complexes with cellular reductants.
The influence of Cr(III) complexes with ascorbic acid, cysteine and glutathione on DNA has been studied spectrophotometrically and chromatographically. The toxic and genotoxic activities of these complexes were also investigated. It was found that these complexes bind to DNA weaker than hexaaqua Cr(III) complexes. It could be explained through the greater strength of the bi- and tridentate ligands coordinated to chromium in comparison to water molecules. The formation of DNA-DNA intermolecular bonds and DNA interstrand cross-linking has been also observed. These complexes were found to be non-toxic and non-genotoxic in the bacterial test.
Gulanowski
B
B
Department of Basic Medical Sciences, Medical Academy, Wrocław, Poland.
Cieślak-Golonka
M
M
Szyba
K
K
Urban
J
J
D001205:Ascorbic Acid / Q000633:toxicity; D002840:Chromates / Q000633:toxicity; D002845:Chromatography; D002857:Chromium / Q000378:metabolism / Q000633:toxicity*; D003432:Cross-Linking Reagents; D003545:Cysteine / Q000633:toxicity; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics; D005978:Glutathione / Q000633:toxicity; D008024:Ligands; D008826:Microbial Sensitivity Tests; D013056:Spectrophotometry, Ultraviolet
D002840:Chromates; D003432:Cross-Linking Reagents; D008024:Ligands; C066097:chromium(V)-glutathione complex; D002857:Chromium; D004247:DNA; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid