Glucose and leucine uptake by rat dorsal root ganglia is not insulin sensitive.
Dorsal root ganglia from rats were incubated with 3-O-methyl-[14C]glucose, and [3H]leucine in the presence or absence of insulin in order to determine whether insulin influences the uptake of glucose and amino acids by the cells of the ganglion. No effect was detected. A significant proportion (38%) of the uptake of [3H]leucine was shown to be inhibited by ouabain and therefore energy dependent, utilizing Na+K(+)-ATPase. The activity of this enzyme is known to be impaired in dorsal root ganglia in diabetic rats, as is the uptake of amino acids; these phenomena are therefore unlikely to be due to a direct effect of reduced circulating insulin levels. The relevance of these findings to theories as to the causation of diabetic neuropathy is discussed.
Patel
N J
NJ
Department of Neurological Science, Royal Free Hospital School of Medicine, London, UK.
Llewelyn
J G
JG
Wright
D W
DW
Thomas
P K
PK
D019325:3-O-Methylglucose; D000818:Animals; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007930:Leucine / Q000493:pharmacokinetics*; D008297:Male; D008757:Methylglucosides / Q000493:pharmacokinetics; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D007328:Insulin; D008757:Methylglucosides; D019325:3-O-Methylglucose; D010042:Ouabain; D007930:Leucine; D005947:Glucose