Use of DNA moderately repetitive sequence to type Aspergillus fumigatus isolates from aspergilloma patients.
Polymorphism of forty-seven sequential clinical isolates from 3 patients with an aspergilloma was analyzed. DNA from each isolate was digested with EcoRI and hybridized in Southern blots with a 32P-labeled nonribosomal DNA repetitive sequence. Most isolates from each patient displayed the same hybridization pattern. Southern blot patterns obtained with DNA repetitive sequences can be used to type clinical isolates of Aspergillus fumigatus and have shown that aspergilloma patients are most probably infected by a single strain.
Girardin
H
H
Unité de Mycologie, Institut Pasteur, Paris, France.
Sarfati
J
J
Kobayashi
H
H
Bouchara
J P
JP
Latgé
J P
JP
D001228:Aspergillosis / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics; D015139:Blotting, Southern; D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D008172:Lung Diseases, Fungal / Q000382:microbiology*; D012091:Repetitive Sequences, Nucleic Acid; D012703:Serotyping
D004271:DNA, Fungal