title: Analysis of hydrolytic products from choline-labeled host cell phospholipids during growth of Rickettsia prowazekii.
abstract: A phospholipase activity has been associated with the interaction of Rickettsia prowazekii with the surface of erythrocytes and competent host cells as well as during the growth of the rickettsiae within their host cells. Both fatty acid and lysophosphatides have been found in the interaction of rickettsiae with the surface of eucaryotic cells; this finding provided strong evidence for the activity of a phospholipase A. However, fatty acids, but not lysophosphatides, were found during the growth of rickettsiae within cells in which the phospholipids had been radiolabeled with oleic acid; this observation left the type of phospholipase activity in doubt. In this study, the water-soluble components of phospholipid hydrolysis by phospholipase A plus lysophospholipase and phospholipase C were determined following the growth of rickettsiae in host cells in which the phospholipids had been radiolabeled with choline. In infected cells relative to mock-infected cells, there was a loss of phosphatidylcholine with a corresponding increase not in lysophosphatidylcholine but in the water-soluble components. There was a large increase in glycerylphosphorylcholine (185%) and a smaller increase in phosphorylcholine (16%). These results indicate that both phospholipase A activity (plus a lysophospholipase activity) and phospholipase C were increased during infection by R. prowazekii and that the former was the predominant activity.
authors.affiliation: Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688.
authors.forename: H H
authors.initials: HH
authors.lastname: Winkler
authors.forename: L
authors.initials: L
authors.lastname: Day
authors.forename: R
authors.initials: R
authors.lastname: Daugherty
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism*; D006868:Hydrolysis; D051379:Mice; D010741:Phospholipases A / Q000502:physiology; D010743:Phospholipids / Q000378:metabolism*; D012283:Rickettsia prowazekii / Q000378:metabolism*; D010738:Type C Phospholipases / Q000502:physiology
chemical_list: D010743:Phospholipids; D010741:Phospholipases A; D010738:Type C Phospholipases; D002794:Choline