title: [Molecular biological study of the vaccinia virus. V. Study of the intracellular localization of the late nonstructural protein 36K].
abstract: Comparative structural analysis of the 36K proteins of vaccinia, ectromelia, cowpox and variola viruses revealed that it is conservative among Orthopoxviruses. The possible role of this protein was suggested. A study of the synthesis kinetics of vaccinia and ectromelia virus 36K proteins established that they belong to late proteins. Electron microscopy of infected cells using protein A labelled with colloidal gold showed that the 36K protein is located in the viroplast and not coupled to virions or any cell organelles.
authors.forename: O I
authors.initials: OI
authors.lastname: Riazankina
authors.forename: I O
authors.initials: IO
authors.lastname: Cheshenko
authors.forename: V V
authors.initials: VV
authors.lastname: Gutorov
authors.forename: S N
authors.initials: SN
authors.lastname: Shchelkunov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D005814:Genes, Viral; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D014616:Vaccinia virus / Q000235:genetics* / Q000648:ultrastructure; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D017361:Viral Nonstructural Proteins
vernacular_title: Molekuliarno-biologicheskoe izuchenie genoma virusa ospovaktsiny. V. Issledovanie vnutrikletochnoĭ lokalizatsii konservativnogo pozdnego nestrukturnogo belka 36K.