title: Purification and characterization of gonadotropin I and II from pituitary glands of tuna (Thunnus obesus)
abstract: The duality of teleost pituitary gonadotropins was established in an advanced marine fish, the tuna (Thunnus obesus). Two different molecular forms of gonadotropins, designated tGTH I and tGTH II, were isolated from an alcoholic extract of pituitary glands following ion-exchange chromatography and reversed-phase HPLC. Both tGTH I and tGTH II stimulated estradiol-17 beta and testosterone production in tuna ovarian follicles in vitro, although responses to tGTH II were significantly greater than those to tGTH I. Each gonadotropin consisted of alpha- and beta-subunits. tGTH I was stable in acidic conditions, whereas tGTH II dissociated into two subunits after acid treatment. Alpha subunits of tGTH I and tGTH II had identical amino acid sequences of 94 amino acid residues. The tGTH I beta and tGTH II beta consisted of 102 and 115 amino acid residues, respectively, and showed 35% sequence identity. tGTH I beta is structurally more similar to salmon GTH I beta than to salmon GTH II beta, whereas tGTH II beta is more similar to salmon GTH II beta. Thus it is evident that the tuna pituitary gland produces two chemically distinct gonadotropins.
authors.affiliation: Central Research Institute, Maruha Corporation, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Okada
authors.forename: I
authors.initials: I
authors.lastname: Kawazoe
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: Y
authors.initials: Y
authors.lastname: Sasamoto
authors.forename: K
authors.initials: K
authors.lastname: Aida
authors.forename: H
authors.initials: H
authors.lastname: Kawauchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002268:Carboxypeptidases / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D006065:Gonadotropins, Pituitary / Q000737:chemistry / Q000302:isolation & purification*; D007700:Kinetics; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010902:Pituitary Gland / Q000737:chemistry*; D017421:Sequence Analysis; D014413:Tuna
chemical_list: D006065:Gonadotropins, Pituitary; C086439:gonadotropin I, tuna; C086440:gonadotropin II, tuna; D002268:Carboxypeptidases; C022633:serine carboxypeptidase