Matrix-assisted laser desorption/ionization of restriction enzyme-digested DNA.
Matrix-assisted laser desorption/ionization, by a time-of-flight mass spectrometer, has been successfully used for detection of restriction enzyme-digested DNA. However, the oligonucleotide segments detected correspond to the molecular weights of single strands.
Tang
K
K
Oak Ridge National Laboratory, Tennessee 37831.
Allman
S L
SL
Chen
C H
CH
Cháng
L Y
LY
Schell
M
M
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded / Q000032:analysis; D006868:Hydrolysis; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D016133:Polymerase Chain Reaction
D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes