[Test of a quick differentiation of selected mycobacteria with pyrolytic gaseous chromatography].
The five mycobacterium strains H37Rv series 363, M. bovis (An5) series 250, M. kansasii series 377, M. intracellulare series 364, M. xenopi series 302 and BCG series 180291 were tested using pyrolytic gas chromatography method. After regeneration of lyophylisate, the strains were incubated using L-J medium in 37 degrees C for 2 to 6 weeks--the period that was necessary to obtain profuse growth. The bacterial mass obtained from the culture was collected in test-tubes and pyrolized after autoclave incubation and dry procedure. The CHROM 5 gas chromatograph together with FID detector was used for detecting the products of pyrolysis. The signal was transmitted from the detector to analog-digital converter A/C and registered by IBM PC/XT computer. 12-15 analysis of each strain were performed. Chromatograms of strains pyrolysis products were recorded as files to the hard disc. This way the standard data base was built. The pyrolysis products of tested strains were compared with data base using BAZCHRO program. E. coli JC 7623 was employed as a standard for comparison of chromatograms. The similarity of chromatograms was presented as percentage. The 85-95% of similarity was obtained as a result of comparison of the same Mycobacteria strains.
Zalewska-Schönthaler
N
N
Zakładu Mikrobiologii Instytutu Gruźlicy i Chorób Płuc, Warszawie.
Lakomy
R
R
Bzdega
J
J
D002849:Chromatography, Gas / Q000379:methods*; D009161:Mycobacterium / Q000145:classification*; D013045:Species Specificity
Próba szybkiego róznicowania wybranych gatunków mykobakterii metoda pirolitycznej chromatografii gazowej.