Establishment of two permanent human bone marrow stromal cell lines with long-term post irradiation feeder capacity.
We describe the establishment of two permanent Simian virus 40-transformed human stromal cell lines, designated L87/4 and L88/5, derived from the bone marrow of a hematologically normal male patient. Both cell lines show a fibroblastoid morphology and do not express hematopoietic cell markers. L87/4 but not L88/5 expresses the macrophage marker CD68. The most remarkable feature of these new stromal cell lines is their ability to persist as growth-arrested adherent feeder cells after ionizing-irradiation at doses up to, and exceeding 20 Gy (L87/4). This renders them particularly useful for studying aspects of feeder dependence of hematopoietic cell development in long-term culture. Both cell lines are able to function as feeder cells, supporting the long-term proliferation of CD34+ human cord blood cells as well as the clonogenic growth of the human Burkitt lymphoma B-cell line BL70.
Thalmeier
K
K
Institut für Experimentelle Hämatologie, GSF-Forschungszentrum für Unwelt und Gesundheit, GmbH, München, Germany.
Meissner
P
P
Reisbach
G
G
Falk
M
M
Brechtel
A
A
Dörmer
P
P
D001854:Bone Marrow Cells; D002460:Cell Line; D005312:Fetal Blood / Q000166:cytology; D006410:Hematopoiesis; D006801:Humans; D008297:Male; D011836:Radiation Tolerance; D017154:Stromal Cells / Q000502:physiology / Q000528:radiation effects