Identification of an active gene by using large-scale cDNA sequencing.
A 3'-directed partial cDNA clone that matches exactly a genomic sequence in GenBank was isolated while collecting transcribed sequences from adult lung by a random approach. This is the first report of active gene identification on genomic sequence without the aid of Northern hybridization.
Itoh
K
K
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Matsubara
K
K
Okubo
K
K
D000328:Adult; D000727:Androgen-Binding Protein; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008168:Lung / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D049488:Phosphatidylethanolamine Binding Protein; D049294:Phospholipid Transfer Proteins; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D000727:Androgen-Binding Protein; D002352:Carrier Proteins; D018076:DNA, Complementary; C511482:PEBP1 protein, human; D049488:Phosphatidylethanolamine Binding Protein; D049294:Phospholipid Transfer Proteins; D016760:Proto-Oncogene Proteins c-fos; D004247:DNA