[The effect of alpha-tocopherol over a wide range of concentrations (10(-2)-10(-17) M) on protein kinase C activity. Connection with proliferation and tumor growth].
The effect of the natural antioxidant alpha-tocopherol (alpha-TL) on protein kinase C (PK-C) from rabbit heart has been studied. alpha-TL inhibits the PK-C activity; the concentration dependence curve has two maxima-at (10(-4)-10(-6) M) and at (10(-11)-10(-15) M). There is a "zone of silence" between these two regions. The shift of the first maximum towards higher alpha-TL concentrations (5 x 10(-3) M) and the decrease in the values of the both inhibition maxima (from 80-90% to 40-60%) was observed for the PK-C preactivated by 12-O-tetradecanoyl-phorbol-13-acetate. The putative role of alpha-TL and lipid hydroperoxides in the regulation of proliferation of normal and tumour cells is discussed.
Pal'mina
N P
NP
Mal'tseva
E L
EL
Kurnakova
N V
NV
Burlakova
E B
EB
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects*; D004789:Enzyme Activation; D008054:Lipid Peroxides / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011817:Rabbits; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000494:pharmacology* / Q000502:physiology
D008054:Lipid Peroxides; D014810:Vitamin E; D016718:Arachidonic Acid; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate
Vliianie alpha-tokoferola v shirokom spektre kontsentratsiĭ (10(-2)- 10(-17)M) na aktivnost' proteinkinazy C. Sviaz' s proliferatsieĭ i opukholevym rostom.