Assembly of LamB and OmpF in deep rough lipopolysaccharide mutants of Escherichia coli K-12.
Assembly of the OmpF and LamB proteins was kinetically retarded in deep rough lipopolysaccharide mutants of Escherichia coli K-12. OmpF assembly was affected at the step of conversion of metastable trimers to stable trimers, whereas LamB assembly was influenced both at the monomer-to-metastable trimer and metastable-to-stable trimer steps. These assembly defects were reversed in the presence of the sfaA1 and sfaB3 suppressor alleles, which were isolated by using ompF assembly mutants.
Laird
M W
MW
Department of Microbiology, Arizona State University, Tempe 85287-2701.
Kloser
A W
AW
Misra
R
R
D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000378:metabolism*; D009154:Mutation / Q000235:genetics / Q000502:physiology; D018272:Porins; D011991:Receptors, Virus / Q000096:biosynthesis / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; D018272:Porins; D011991:Receptors, Virus; C016532:maltoporins