Increased interleukin-4 production in response to mast cell mediators and human type I collagen in patients with rheumatoid arthritis.
Mast cell synovial hyperplasia can occur in patients with rheumatoid arthritis. Histamine can accelerate synovitis and heparin can inhibit lymphocyte function. Since interleukin-4 (IL-4) can stimulate murine mast cell and IgE synthesis, we determined whether mast cell mediators and anti-IL-4 might effect lymphocyte proliferation from patients with rheumatoid arthritis. Twenty-four patients with rheumatoid arthritis and nine normal controls were evaluated by history, physical examination, physician and patient-assessed joint and allergic symptoms, and diary scores. An IL-2-driven-T cell (3H) Tdr proliferation assay with monoclonal anti-IL-4 and a sensitive ELISA were performed with isolated peripheral blood mononuclear cells (PBMC) with 10 micrograms/mL of either concanavalin A (Con A), type I human collagen, or heparin and 10(-6) M histamine. Increased lymphocyte proliferation indices with Con A (mean 21.69 +/- 4.9; 6.54 +/- 3.2 normal controls), type I human collagen (mean 2.17 +/- 0.52; 1.1 +/- .17, normal controls), histamine (mean 1.66 +/- .36; 0.62 +/- 0.08, normal controls), heparin (mean 1.61 +/- .38; 0.69 +/- .11, normal controls) occurred in peripheral blood mononuclear cells from all patients with rheumatoid arthritis compared with normal controls (P < .0236 to .0015) which was inhibited in 32% of peripheral blood mononuclear cells by anti-IL-4. Increased IL-4 ELISA levels in cultured supernatants were noted with heparin (P < .25) and collagen (P < .05).(ABSTRACT TRUNCATED AT 250 WORDS)
Frieri
M
M
Department of Pathology, Nassau County Medical Center, State University of New York at Stony Brook.
Agarwal
K
K
Datar
A
A
Trotta
P
P
D000328:Adult; D000368:Aged; D000906:Antibodies / Q000494:pharmacology; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000378:metabolism*; D003094:Collagen / Q000502:physiology*; D005260:Female; D006493:Heparin / Q000502:physiology*; D006632:Histamine / Q000502:physiology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology / Q000378:metabolism; D007073:Immunoglobulin E / Q000097:blood; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology
D000906:Antibodies; D015847:Interleukin-4; D007073:Immunoglobulin E; D006632:Histamine; D006493:Heparin; D003094:Collagen