Induction of expression of adhesion molecules on an eosinophilic cell line (EoL-1) by the supernatant of lymphocytes stimulated with specific allergen from asthmatic patients.
Recently, adhesion molecules have been considered to play an important role in inflammatory processes in bronchial asthma. To extend our understanding of the high-intensity expression of adhesion molecules (CR3, LFA-1 alpha, LFA-1 beta, ICAM-1) on hypodense eosinophils, which was observed in our previous study, we examined whether the supernatant of lymphocytes from mite-allergic asthmatic patients is involved in adhesion molecule expression using an eosinophilic cell line (EoL-1). These characteristics of adhesion molecule expression were induced by the supernatant of lymphocytes obtained from mite-allergic asthmatic patients cultured with specific allergen as well as a combination of recombinant cytokines (IL-3, GM-CSF, IL-5). Thus, we could conclude that some lymphokines produced by specific allergen in asthma might be involved in the high-intensity expression of adhesion molecules on hypodense eosinophils in asthma or allergic disorders.
Chihara
J
J
Fourth Department of Internal Medicine, Kinki University School of Medicine, Osakasayama, Japan.
Kurachi
D
D
Hayashi
N
N
Yamamoto
T
T
Higashimoto
I
I
Kakazu
T
T
Nakajima
S
S
D000485:Allergens / Q000009:adverse effects; D000818:Animals; D001249:Asthma / Q000097:blood* / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002460:Cell Line; D004804:Eosinophils / Q000737:chemistry*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D006801:Humans; D007377:Interleukin-3 / Q000502:physiology; D015848:Interleukin-5 / Q000502:physiology; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D008214:Lymphocytes / Q000276:immunology; D016177:Macrophage-1 Antigen / Q000502:physiology; D008925:Mites / Q000276:immunology
D000485:Allergens; D015815:Cell Adhesion Molecules; D007377:Interleukin-3; D015848:Interleukin-5; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor