Biological monitoring of occupational exposure to n-hexane by exhaled air analysis and urinalysis.
To compare two methods of biological monitoring for the evaluation of risk of occupational exposure to n-hexane, we analyze the relationship between environmental exposure to this solvent and urinary excretion of 2,5-hexanedione and n-hexane in exhaled air in 69 workers employed in the shoe industry. Environmental exposure to the solvent was monitored with personal diffusive samplers, which were desorbed with carbon sulfide and analyzed by gas chromatography. To measure 2,5-hexanedione, urine was subjected to acid hydrolysis, separation in octadecyl silane columns, elution with 5% aqueous acetonitrile solution and extraction with dichloromethane, followed by gas chromatography. In exhaled air, n-hexane was measured with a sampling system that permitted concentration of aliquots of end-exhaled air (alveolar air) from one or more exhalations in a tube packed with activated charcoal, which was then desorbed with carbon sulfide and analyzed by gas chromatography. Concentrations of n-hexane in breathing zone air were significantly correlated with urinary concentrations of 2,5-hexanedione (r = 0.88) and with exhaled air n-hexane (r = 0.86); in addition, the two biological indicators correlated significantly (r = 0.70). Analyses in both exhaled air and urine were thus considered useful for biological monitoring of the risk of exposure to n-hexane.
Periago
J F
JF
National Institute of Occupational Safety and Hygiene, Murcia, Spain.
Cardona
A
A
Marhuenda
D
D
Roel
J
J
Villanueva
M
M
Marti
J
J
Luna
A
A
D000269:Adhesives; D000293:Adolescent; D000328:Adult; D001944:Breath Tests; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D006586:Hexanes / Q000009:adverse effects / Q000493:pharmacokinetics*; D006588:Hexanones / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D012775:Shoes
D000269:Adhesives; D006586:Hexanes; D006588:Hexanones; C026385:n-hexane; C011269:2,5-hexanedione