Prostaglandin E (PGE) and interleukin-1 beta (IL-1 beta) levels in gingival crevicular fluid during human orthodontic tooth movement.
The purpose of this study was to examine gingival crevicular fluid (GCF) levels of two potent bone resorbing mediators, prostaglandin E (PGE) and interleukin-1 beta (IL-1 beta), during human orthodontic tooth movement. The study included 10 patients, each having one treatment tooth undergoing orthodontic movement and a contralateral control tooth. The GCF was sampled at control sites and treatment (compression) sites before activation and a 1, 24, 48, and 168 hours. Prevention of plaque-induced inflammation allowed this study to focus on the dynamics of mechanically stimulated PGE and IL-1 beta GCF levels. The PGE and IL-1 beta levels were determined with radioimmunoassay. At 1 and 24 hours, mean GCF IL-1 beta levels were significantly elevated at treatment teeth (8.9 +/- 2.0 and 19.2 +/- 6.0 pg, respectively) compared with control teeth (2.0 +/- 1.1 pg, p = 0.0049, and 2.9 +/- 1.0 pg, p = 0.0209, respectively). The GCF levels of PGE for the treatment teeth were significantly higher at 24 and 48 hours (108.9 +/- 11.9 and 97.9 +/- 7.3 pg) than the control teeth (61.8 +/- 7.2 pg, p = 0.0071, and 70.8 +/- 7.4 pg, p = 0.0021, respectively). The GCF levels of PGE and IL-1 beta remained at baseline levels throughout the study for the control teeth, whereas significant elevations from baseline in GCF IL-1 beta (24 hours) and PGE levels (24 and 48 hours) were observed over time in the treatment teeth (p < or = 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Grieve
W G
WG
Department of Periodontics, University of Iowa, Iowa City.
Johnson
G K
GK
Moore
R N
RN
Reinhardt
R A
RA
DuBois
L M
LM
D000328:Adult; D016301:Alveolar Bone Loss / Q000209:etiology* / Q000378:metabolism*; D000704:Analysis of Variance; D003799:Dental Stress Analysis / Q000379:methods; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D011458:Prostaglandins E / Q000032:analysis / Q000096:biosynthesis*; D011863:Radioimmunoassay; D014087:Tooth Movement Techniques / Q000009:adverse effects*
D007375:Interleukin-1; D011458:Prostaglandins E