title: Human immunodeficiency virus type 1 Rev-responsive element RNA binds to host cell-specific proteins.
abstract: RNase protection-gel retention studies show human host cell-specific ribonucleoprotein complexes with human immunodeficiency virus type 1 Rev-responsive element (RRE) RNA. Nuclear proteins from rodent or murine cells appear to lack the ability to form these complexes. Human-mouse somatic cell hybrids retaining a single human chromosome, either 6 or 12, form the RRE-nuclear-protein complexes. One of the complexes requires the entire RRE RNA, while the other needs RRE RNA stem-loops 1 and 2 only. Two major proteins with molecular masses of 120 and 62 kDa specifically bind to RRE RNA. Rodent cells (CHO) either lack or contain small amounts of these RRE-binding proteins.
authors.affiliation: Department of Biochemistry and Molecular Biology, George Washington University Medical Center, Washington, D.C. 20037.
authors.forename: R R
authors.initials: RR
authors.lastname: Shukla
authors.forename: P L
authors.initials: PL
authors.lastname: Kimmel
authors.forename: A
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000818:Animals; D003432:Cross-Linking Reagents; D015752:Genes, env; D015497:HIV-1 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012261:Ribonucleoproteins / Q000378:metabolism / Q000528:radiation effects; D013045:Species Specificity; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins