title: Regional saturation mutagenesis as an approach to identification of substrate specificity determinants in cytochrome P450 BM3.
authors.affiliation: Department of Biochemistry, University of Glasgow, U.K.
authors.forename: A W
authors.initials: AW
authors.lastname: Munro
authors.forename: J R
authors.initials: JR
authors.lastname: Coggins
authors.forename: J G
authors.initials: JG
authors.lastname: Lindsay
mesh_terms: D000595:Amino Acid Sequence; D001410:Bacillus megaterium / Q000201:enzymology*; D001426:Bacterial Proteins; D017124:Conserved Sequence; D003545:Cysteine; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D017931:DNA Primers; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009251:NADPH-Ferrihemoprotein Reductase; D017433:Protein Structure, Secondary; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases; D003545:Cysteine