Microcrystals of the beta 1 isoenzyme of protein kinase C: an electron microscopic study.
Microcrystals of protein kinase C beta 1 have been grown from solutions of poly(ethylene glycol). Image analysis of electron micrographs of the protein crystals, which diffracted to 5.0 nm, revealed p3 symmetry with a unit cell of about 10.3 nm x 10.3 nm. The electron stain-excluding densities showed a three-domain ring structure in projection, giving kidney bean-shaped molecules of about 7.0 nm x 4.5 nm diameter, packed as trimers. The implications of these observations for the function of the enzyme are discussed.
Newman
R H
RH
Laboratory of Protein Structure, Imperial Cancer Research Fund, London, U.K.
Carpenter
E
E
Freemont
P S
PS
Blundell
T L
TL
Parker
P J
PJ
D003461:Crystallography; D007527:Isoenzymes / Q000737:chemistry / Q000648:ultrastructure*; D008854:Microscopy, Electron; D011493:Protein Kinase C / Q000737:chemistry / Q000648:ultrastructure*
D007527:Isoenzymes; D011493:Protein Kinase C