T cell receptor V beta 15 dominates the antiacetylcholine receptor response in Lewis rat T cell lines.
Ten Lewis rat T cell lines responsive to Torpedo californica acetylcholine receptor (AChR) were assayed for TCR V beta usage. All lines were CD4+, OX-22-, and exhibited reactivity to one or more AChR chains. Several different V beta s were expressed by these lines, but V beta 15 was dominant in 5 of 10 lines. Unique CDR3 sequences were observed among the 10 lines, although three of the V beta 15 rearrangements used J beta 1.4. These data suggest that V beta 15+ T cells are selected in the in vitro response to the antigenically complex AChR in the Lewis rat.
Smith
L R
LR
Immune Response Corporation, Carlsbad, CA 92008.
Kono
D H
DH
Asthana
D
D
Balderas
R S
RS
Fujii
Y
Y
Lindstrom
J
J
Theofilopoulos
A N
AN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004195:Disease Models, Animal; D015870:Gene Expression; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D007114:Immunization; D008969:Molecular Sequence Data; D009157:Myasthenia Gravis / Q000209:etiology / Q000235:genetics / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D011950:Receptors, Cholinergic / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014101:Torpedo
D016693:Receptors, Antigen, T-Cell, alpha-beta; D011950:Receptors, Cholinergic; D004247:DNA